LOCUS       DAJTRH010000001       136266 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTRH010000001 DAJTRH010000000
VERSION     DAJTRH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..1367
                     /gene="cscA"
                     /locus_tag="OO339_000001"
     CDS             <1..1367
                     /gene="cscA"
                     /locus_tag="OO339_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5924798.1"
                     /translation="FYPHFHLAPPAGWMNDPNGLIWFNDRYHAFYQHHPMSEHWGPMH
                     WGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSAVDDNGVLSLIYTGHVWLDGAGND
                     DAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFRDPKVWREADTWWMVVGAKDPGNT
                     GQILLYRGSSLREWAFDRVLAHADAGESYMWECPDFFSLGDQHYLMFSPQGMNAEGYS
                     YRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDFYAPQSFLAKDGRRIVIGWMDMWE
                     SPMPSKREGWAGCMTLARELSESNGKLLQRPVHEAESLRQQHQSVSPRRISNKYVLQE
                     NAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDNQSERLILWRYYPHENLDGYRSIP
                     LPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYPQPEERELSLYASHGVAVLQHGAL
                     WLLG"
     gene            complement(1375..2433)
                     /locus_tag="OO339_000002"
     CDS             complement(1375..2433)
                     /locus_tag="OO339_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5924799.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRTAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2601..2819
                     /locus_tag="OO339_000003"
                     /pseudo
     CDS             2601..2819
                     /locus_tag="OO339_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2837..4165
                     /gene="dsdA"
                     /locus_tag="OO339_000004"
     CDS             2837..4165
                     /gene="dsdA"
                     /locus_tag="OO339_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5924800.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4273..5811)
                     /gene="emrY"
                     /locus_tag="OO339_000005"
     CDS             complement(4273..5811)
                     /gene="emrY"
                     /locus_tag="OO339_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5924801.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5811..6974)
                     /gene="emrK"
                     /locus_tag="OO339_000006"
     CDS             complement(5811..6974)
                     /gene="emrK"
                     /locus_tag="OO339_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5924802.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7390..8004
                     /gene="evgA"
                     /locus_tag="OO339_000007"
     CDS             7390..8004
                     /gene="evgA"
                     /locus_tag="OO339_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5924803.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8009..11602
                     /gene="evgS"
                     /locus_tag="OO339_000008"
     CDS             8009..11602
                     /gene="evgS"
                     /locus_tag="OO339_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5924804.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11658..12803)
                     /gene="yfdE"
                     /locus_tag="OO339_000009"
     CDS             complement(11658..12803)
                     /gene="yfdE"
                     /locus_tag="OO339_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5924805.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12877..13821)
                     /gene="yfdV"
                     /locus_tag="OO339_000010"
     CDS             complement(12877..13821)
                     /gene="yfdV"
                     /locus_tag="OO339_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5924806.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13891..15585)
                     /gene="oxc"
                     /locus_tag="OO339_000011"
     CDS             complement(13891..15585)
                     /gene="oxc"
                     /locus_tag="OO339_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5924807.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15639..16889)
                     /gene="frc"
                     /locus_tag="OO339_000012"
     CDS             complement(15639..16889)
                     /gene="frc"
                     /locus_tag="OO339_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5924808.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17402..18025)
                     /locus_tag="OO339_000013"
     CDS             complement(17402..18025)
                     /locus_tag="OO339_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5924809.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18322..18597
                     /locus_tag="OO339_000014"
     CDS             18322..18597
                     /locus_tag="OO339_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924810.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18674..18916)
                     /locus_tag="OO339_000015"
     CDS             complement(18674..18916)
                     /locus_tag="OO339_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5924811.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19269..20189
                     /gene="lpxP"
                     /locus_tag="OO339_000016"
     CDS             19269..20189
                     /gene="lpxP"
                     /locus_tag="OO339_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5924812.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20544..20615
                     /gene="ypdK"
                     /locus_tag="OO339_000017"
     CDS             20544..20615
                     /gene="ypdK"
                     /locus_tag="OO339_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5924813.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20680..21918)
                     /gene="alaC"
                     /locus_tag="OO339_000018"
     CDS             complement(20680..21918)
                     /gene="alaC"
                     /locus_tag="OO339_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5924814.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22295..23808
                     /locus_tag="OO339_000019"
                     /pseudo
     CDS             22295..23808
                     /locus_tag="OO339_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            23823..24557
                     /gene="ypdB"
                     /locus_tag="OO339_000020"
     CDS             23823..24557
                     /gene="ypdB"
                     /locus_tag="OO339_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5924815.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24570..25427
                     /locus_tag="OO339_000021"
                     /pseudo
     CDS             24570..25427
                     /locus_tag="OO339_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25430..27925)
                     /gene="ptsP"
                     /locus_tag="OO339_000022"
     CDS             complement(25430..27925)
                     /gene="ptsP"
                     /locus_tag="OO339_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5924816.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(27950..28987)
                     /gene="ypdE"
                     /locus_tag="OO339_000023"
     CDS             complement(27950..28987)
                     /gene="ypdE"
                     /locus_tag="OO339_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5924817.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(28987..30070)
                     /gene="ypdF"
                     /locus_tag="OO339_000024"
                     /pseudo
     CDS             complement(28987..30070)
                     /gene="ypdF"
                     /locus_tag="OO339_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30085..31332)
                     /locus_tag="OO339_000025"
     CDS             complement(30085..31332)
                     /locus_tag="OO339_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5924818.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31354..31680)
                     /locus_tag="OO339_000026"
     CDS             complement(31354..31680)
                     /locus_tag="OO339_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5924819.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31899..32864)
                     /gene="glk"
                     /locus_tag="OO339_000027"
     CDS             complement(31899..32864)
                     /gene="glk"
                     /locus_tag="OO339_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5924820.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLRPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33068..34324
                     /locus_tag="OO339_000028"
     CDS             33068..34324
                     /locus_tag="OO339_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5924821.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34439..34765
                     /locus_tag="OO339_000029"
     CDS             34439..34765
                     /locus_tag="OO339_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5924822.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34906..36144)
                     /locus_tag="OO339_000030"
     CDS             complement(34906..36144)
                     /locus_tag="OO339_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5924823.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36480..37682
                     /gene="nupC"
                     /locus_tag="OO339_000031"
     CDS             36480..37682
                     /gene="nupC"
                     /locus_tag="OO339_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5924824.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37732..39844)
                     /locus_tag="OO339_000032"
                     /pseudo
     CDS             complement(37732..39844)
                     /locus_tag="OO339_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40052..40127)
                     /locus_tag="OO339_000033"
     tRNA            complement(40052..40127)
                     /locus_tag="OO339_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40092..40094),aa:Ala,seq:ggc)
     gene            complement(40167..40242)
                     /locus_tag="OO339_000034"
     tRNA            complement(40167..40242)
                     /locus_tag="OO339_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40207..40209),aa:Ala,seq:ggc)
     gene            40463..40822
                     /locus_tag="OO339_000035"
     CDS             40463..40822
                     /locus_tag="OO339_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5924825.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40824..41216
                     /locus_tag="OO339_000036"
     CDS             40824..41216
                     /locus_tag="OO339_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5924826.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41268..42683)
                     /gene="gltX"
                     /locus_tag="OO339_000037"
     CDS             complement(41268..42683)
                     /gene="gltX"
                     /locus_tag="OO339_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5924827.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            42942..43017
                     /locus_tag="OO339_000038"
     tRNA            42942..43017
                     /locus_tag="OO339_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42975..42977,aa:Val,seq:tac)
     gene            43062..43137
                     /locus_tag="OO339_000039"
     tRNA            43062..43137
                     /locus_tag="OO339_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43095..43097,aa:Val,seq:tac)
     gene            43142..43217
                     /locus_tag="OO339_000040"
     tRNA            43142..43217
                     /locus_tag="OO339_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43175..43177,aa:Lys,seq:ttt)
     gene            43248..43373
                     /locus_tag="OO339_000041"
     ncRNA           43248..43373
                     /ncRNA_class="other"
                     /locus_tag="OO339_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43493..43834
                     /locus_tag="OO339_000042"
     CDS             43493..43834
                     /locus_tag="OO339_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5924828.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(43825..44751)
                     /locus_tag="OO339_000043"
     CDS             complement(43825..44751)
                     /locus_tag="OO339_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5924829.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            44841..45839
                     /locus_tag="OO339_000044"
     CDS             44841..45839
                     /locus_tag="OO339_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5924830.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(45836..46054)
                     /locus_tag="OO339_000045"
     CDS             complement(45836..46054)
                     /locus_tag="OO339_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5924831.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46056..48071)
                     /gene="ligA"
                     /locus_tag="OO339_000046"
     CDS             complement(46056..48071)
                     /gene="ligA"
                     /locus_tag="OO339_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5924832.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48142..49128)
                     /gene="zipA"
                     /locus_tag="OO339_000047"
     CDS             complement(48142..49128)
                     /gene="zipA"
                     /locus_tag="OO339_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5924833.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49358..50119
                     /gene="cysZ"
                     /locus_tag="OO339_000048"
     CDS             49358..50119
                     /gene="cysZ"
                     /locus_tag="OO339_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5924834.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50304..51275
                     /gene="cysK"
                     /locus_tag="OO339_000049"
     CDS             50304..51275
                     /gene="cysK"
                     /locus_tag="OO339_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5924835.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51659..51916
                     /gene="ptsH"
                     /locus_tag="OO339_000050"
     CDS             51659..51916
                     /gene="ptsH"
                     /locus_tag="OO339_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5924836.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            51961..53688
                     /gene="ptsI"
                     /locus_tag="OO339_000051"
     CDS             51961..53688
                     /gene="ptsI"
                     /locus_tag="OO339_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5924837.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53729..54238
                     /gene="crr"
                     /locus_tag="OO339_000052"
     CDS             53729..54238
                     /gene="crr"
                     /locus_tag="OO339_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5924838.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54280..55131)
                     /gene="pdxK"
                     /locus_tag="OO339_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54280..55131)
                     /gene="pdxK"
                     /locus_tag="OO339_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5924839.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55236..55610
                     /locus_tag="OO339_000054"
     CDS             55236..55610
                     /locus_tag="OO339_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5924840.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55643..56366
                     /locus_tag="OO339_000055"
                     /pseudo
     CDS             55643..56366
                     /locus_tag="OO339_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56570..57481)
                     /gene="cysM"
                     /locus_tag="OO339_000056"
     CDS             complement(56570..57481)
                     /gene="cysM"
                     /locus_tag="OO339_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5924841.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57615..58712)
                     /gene="cysA"
                     /locus_tag="OO339_000057"
     CDS             complement(57615..58712)
                     /gene="cysA"
                     /locus_tag="OO339_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5924842.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58702..59577)
                     /gene="cysW"
                     /locus_tag="OO339_000058"
     CDS             complement(58702..59577)
                     /gene="cysW"
                     /locus_tag="OO339_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5924843.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59577..60410)
                     /gene="cysT"
                     /locus_tag="OO339_000059"
     CDS             complement(59577..60410)
                     /gene="cysT"
                     /locus_tag="OO339_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5924844.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60410..61426)
                     /gene="cysP"
                     /locus_tag="OO339_000060"
     CDS             complement(60410..61426)
                     /gene="cysP"
                     /locus_tag="OO339_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5924845.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61730..62521)
                     /gene="ucpA"
                     /locus_tag="OO339_000061"
     CDS             complement(61730..62521)
                     /gene="ucpA"
                     /locus_tag="OO339_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5924846.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62708..63508)
                     /gene="murR"
                     /locus_tag="OO339_000062"
     CDS             complement(62708..63508)
                     /gene="murR"
                     /locus_tag="OO339_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5924847.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63672..64568
                     /gene="murQ"
                     /locus_tag="OO339_000063"
     CDS             63672..64568
                     /gene="murQ"
                     /locus_tag="OO339_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5924848.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64572..65996
                     /gene="murP"
                     /locus_tag="OO339_000064"
     CDS             64572..65996
                     /gene="murP"
                     /locus_tag="OO339_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5924849.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66176..67075)
                     /gene="yfeX"
                     /locus_tag="OO339_000065"
     CDS             complement(66176..67075)
                     /gene="yfeX"
                     /locus_tag="OO339_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5924850.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67171..67746)
                     /locus_tag="OO339_000066"
     CDS             complement(67171..67746)
                     /locus_tag="OO339_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5924851.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(67807..68256)
                     /locus_tag="OO339_000067"
     CDS             complement(67807..68256)
                     /locus_tag="OO339_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5924852.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68243..68668)
                     /locus_tag="OO339_000068"
     CDS             complement(68243..68668)
                     /locus_tag="OO339_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5924853.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            68882..69751
                     /gene="amiA"
                     /locus_tag="OO339_000069"
     CDS             68882..69751
                     /gene="amiA"
                     /locus_tag="OO339_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5924854.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69755..70654
                     /gene="hemF"
                     /locus_tag="OO339_000070"
     CDS             69755..70654
                     /gene="hemF"
                     /locus_tag="OO339_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5924855.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70660..71712)
                     /gene="eutR"
                     /locus_tag="OO339_000071"
                     /pseudo
     CDS             complement(70660..71712)
                     /gene="eutR"
                     /locus_tag="OO339_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71758..72258)
                     /gene="eutK"
                     /locus_tag="OO339_000072"
     CDS             complement(71758..72258)
                     /gene="eutK"
                     /locus_tag="OO339_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5924856.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72271..72930)
                     /gene="eutL"
                     /locus_tag="OO339_000073"
     CDS             complement(72271..72930)
                     /gene="eutL"
                     /locus_tag="OO339_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5924857.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74112..74903
                     /locus_tag="OO339_000074"
     CDS             74112..74903
                     /locus_tag="OO339_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924858.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75623..76018
                     /locus_tag="OO339_000075"
     CDS             75623..76018
                     /locus_tag="OO339_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5924859.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76036..76299
                     /locus_tag="OO339_000076"
     CDS             76036..76299
                     /locus_tag="OO339_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924860.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76322..76864
                     /locus_tag="OO339_000077"
     CDS             76322..76864
                     /locus_tag="OO339_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924861.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77003..77290
                     /locus_tag="OO339_000078"
     CDS             77003..77290
                     /locus_tag="OO339_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924862.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77329..77535
                     /locus_tag="OO339_000079"
     CDS             77329..77535
                     /locus_tag="OO339_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924863.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            77847..78332
                     /locus_tag="OO339_000080"
     CDS             77847..78332
                     /locus_tag="OO339_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5924864.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78313..78591
                     /locus_tag="OO339_000081"
     CDS             78313..78591
                     /locus_tag="OO339_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924865.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78584..80044
                     /locus_tag="OO339_000082"
     CDS             78584..80044
                     /locus_tag="OO339_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5924866.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80078..80887
                     /gene="murI"
                     /locus_tag="OO339_000083"
     CDS             80078..80887
                     /gene="murI"
                     /locus_tag="OO339_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5924867.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81006..81700)
                     /locus_tag="OO339_000084"
     CDS             complement(join(81006..81454,81454..81700))
                     /locus_tag="OO339_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5924868.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82219..83592)
                     /gene="eutA"
                     /locus_tag="OO339_000085"
     CDS             complement(82219..83592)
                     /gene="eutA"
                     /locus_tag="OO339_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5924869.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83567..84046)
                     /gene="eutP"
                     /locus_tag="OO339_000086"
     CDS             complement(83567..84046)
                     /gene="eutP"
                     /locus_tag="OO339_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5924870.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84059..84394)
                     /gene="eutS"
                     /locus_tag="OO339_000087"
     CDS             complement(84059..84394)
                     /gene="eutS"
                     /locus_tag="OO339_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5924871.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84687..86966)
                     /gene="maeB"
                     /locus_tag="OO339_000088"
     CDS             complement(84687..86966)
                     /gene="maeB"
                     /locus_tag="OO339_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5924872.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87255..88205
                     /gene="tal"
                     /locus_tag="OO339_000089"
     CDS             87255..88205
                     /gene="tal"
                     /locus_tag="OO339_000089"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5924873.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88225..90228
                     /gene="tkt"
                     /locus_tag="OO339_000090"
     CDS             88225..90228
                     /gene="tkt"
                     /locus_tag="OO339_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5924874.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90331..91374)
                     /locus_tag="OO339_000091"
     CDS             complement(90331..91374)
                     /locus_tag="OO339_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5924875.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91500..92075)
                     /gene="nudK"
                     /locus_tag="OO339_000092"
     CDS             complement(91500..92075)
                     /gene="nudK"
                     /locus_tag="OO339_000092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5924876.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92143..94122)
                     /gene="aegA"
                     /locus_tag="OO339_000093"
     CDS             complement(92143..94122)
                     /gene="aegA"
                     /locus_tag="OO339_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5924877.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94328..96045
                     /gene="narQ"
                     /locus_tag="OO339_000094"
                     /pseudo
     CDS             94328..96045
                     /gene="narQ"
                     /locus_tag="OO339_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96247..99360
                     /gene="acrD"
                     /locus_tag="OO339_000095"
     CDS             96247..99360
                     /gene="acrD"
                     /locus_tag="OO339_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5924878.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99459..99518)
                     /gene="ypfM"
                     /locus_tag="OO339_000096"
     CDS             complement(99459..99518)
                     /gene="ypfM"
                     /locus_tag="OO339_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5924879.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            99899..100255
                     /locus_tag="OO339_000097"
     CDS             99899..100255
                     /locus_tag="OO339_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5924880.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100259..101386
                     /gene="dapE"
                     /locus_tag="OO339_000098"
     CDS             100259..101386
                     /gene="dapE"
                     /locus_tag="OO339_000098"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5924881.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101414..101614
                     /locus_tag="OO339_000099"
     CDS             101414..101614
                     /locus_tag="OO339_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5924882.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101724..102422)
                     /gene="ypfH"
                     /locus_tag="OO339_000100"
     CDS             complement(101724..102422)
                     /gene="ypfH"
                     /locus_tag="OO339_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5924883.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102496..104511)
                     /gene="tmcA"
                     /locus_tag="OO339_000101"
     CDS             complement(102496..104511)
                     /gene="tmcA"
                     /locus_tag="OO339_000101"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5924884.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104526..105389)
                     /locus_tag="OO339_000102"
     CDS             complement(104526..105389)
                     /locus_tag="OO339_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5924885.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105557..106270)
                     /gene="purC"
                     /locus_tag="OO339_000103"
     CDS             complement(105557..106270)
                     /gene="purC"
                     /locus_tag="OO339_000103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5924886.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106483..107517)
                     /gene="bamC"
                     /locus_tag="OO339_000104"
     CDS             complement(106483..107517)
                     /gene="bamC"
                     /locus_tag="OO339_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5924887.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107534..108412)
                     /gene="dapA"
                     /locus_tag="OO339_000105"
     CDS             complement(107534..108412)
                     /gene="dapA"
                     /locus_tag="OO339_000105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5924888.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108558..109130
                     /locus_tag="OO339_000106"
     CDS             108558..109130
                     /locus_tag="OO339_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5924889.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109130..109600
                     /gene="bcp"
                     /locus_tag="OO339_000107"
     CDS             109130..109600
                     /gene="bcp"
                     /locus_tag="OO339_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5924890.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            109853..110470
                     /gene="hyfA"
                     /locus_tag="OO339_000108"
     CDS             109853..110470
                     /gene="hyfA"
                     /locus_tag="OO339_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5924891.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110470..112487
                     /gene="hyfB"
                     /locus_tag="OO339_000109"
                     /pseudo
     CDS             110470..112487
                     /gene="hyfB"
                     /locus_tag="OO339_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112498..113445
                     /gene="hyfC"
                     /locus_tag="OO339_000110"
     CDS             112498..113445
                     /gene="hyfC"
                     /locus_tag="OO339_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5924892.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGARLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113462..114901
                     /gene="hyfD"
                     /locus_tag="OO339_000111"
     CDS             113462..114901
                     /gene="hyfD"
                     /locus_tag="OO339_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5924893.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            114913..115563
                     /gene="hyfE"
                     /locus_tag="OO339_000112"
     CDS             114913..115563
                     /gene="hyfE"
                     /locus_tag="OO339_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5924894.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115568..117160
                     /gene="hyfF"
                     /locus_tag="OO339_000113"
     CDS             115568..117160
                     /gene="hyfF"
                     /locus_tag="OO339_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5924895.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            117150..118864
                     /gene="hyfG"
                     /locus_tag="OO339_000114"
                     /pseudo
     CDS             117150..118864
                     /gene="hyfG"
                     /locus_tag="OO339_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            118874..119419
                     /gene="hyfH"
                     /locus_tag="OO339_000115"
     CDS             118874..119419
                     /gene="hyfH"
                     /locus_tag="OO339_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5924896.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119416..120174
                     /gene="hyfI"
                     /locus_tag="OO339_000116"
     CDS             119416..120174
                     /gene="hyfI"
                     /locus_tag="OO339_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5924897.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            120167..120580
                     /gene="hyfJ"
                     /locus_tag="OO339_000117"
     CDS             120167..120580
                     /gene="hyfJ"
                     /locus_tag="OO339_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5924898.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120610..122439
                     /gene="hyfR"
                     /locus_tag="OO339_000118"
     CDS             120610..122439
                     /gene="hyfR"
                     /locus_tag="OO339_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5924899.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122440..122622
                     /locus_tag="OO339_000119"
     CDS             122440..122622
                     /locus_tag="OO339_000119"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5924900.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122644..123516
                     /locus_tag="OO339_000120"
                     /pseudo
     CDS             122644..123516
                     /locus_tag="OO339_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123610..124671)
                     /locus_tag="OO339_000121"
     CDS             complement(123610..124671)
                     /locus_tag="OO339_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5924901.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            124884..126347
                     /gene="bepA"
                     /locus_tag="OO339_000122"
     CDS             124884..126347
                     /gene="bepA"
                     /locus_tag="OO339_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5924902.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            126368..126727
                     /gene="arsC"
                     /locus_tag="OO339_000123"
     CDS             126368..126727
                     /gene="arsC"
                     /locus_tag="OO339_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5924903.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(126860..127606)
                     /gene="hda"
                     /locus_tag="OO339_000124"
     CDS             complement(126860..127606)
                     /gene="hda"
                     /locus_tag="OO339_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5924904.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127656..128945)
                     /gene="uraA"
                     /locus_tag="OO339_000125"
     CDS             complement(127656..128945)
                     /gene="uraA"
                     /locus_tag="OO339_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5924905.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(129031..129657)
                     /gene="upp"
                     /locus_tag="OO339_000126"
     CDS             complement(129031..129657)
                     /gene="upp"
                     /locus_tag="OO339_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5924906.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            129981..131018
                     /gene="purM"
                     /locus_tag="OO339_000127"
     CDS             129981..131018
                     /gene="purM"
                     /locus_tag="OO339_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5924907.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            131018..131656
                     /gene="purN"
                     /locus_tag="OO339_000128"
     CDS             131018..131656
                     /gene="purN"
                     /locus_tag="OO339_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5924908.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            131828..133894
                     /gene="ppk1"
                     /locus_tag="OO339_000129"
     CDS             131828..133894
                     /gene="ppk1"
                     /locus_tag="OO339_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5924909.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            133899..135440
                     /gene="ppx"
                     /locus_tag="OO339_000130"
     CDS             133899..135440
                     /gene="ppx"
                     /locus_tag="OO339_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5924910.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135479..>136266)
                     /gene="pdeF"
                     /locus_tag="OO339_000131"
     CDS             complement(135479..>136266)
                     /gene="pdeF"
                     /locus_tag="OO339_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5924911.1"
                     /translation="QRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLR
                     MKGENDELIGPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPT
                     SVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAID
                     DFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEY
                     VENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        33988 a        35075 c        33504 g        33699 t
ORIGIN      
        1 ctttctatcc ccattttcac ctcgcgcctc ctgccggatg gatgaacgat ccaaacggcc
       61 tgatctggtt taacgatcgt tatcacgcgt tttatcaaca tcacccgatg agcgaacact
      121 ggggaccaat gcactgggga catgccacca gtgacgatat gatccactgg cagcatgagc
      181 ctattgcgct agcgctagga gacgataatg acaaagacgg gtgtttttca ggtagtgctg
      241 tcgatgacaa tggtgtcctc tcacttatct acaccggaca cgtctggctc gatggtgcag
      301 gtaatgacga tgcaattcgc gaagtacaat gtctggctac cagtcgggat ggtattcatt
      361 tcgagaaaca gggtgtgatc ctcactccac cagaaggaat catgcacttc cgcgatccta
      421 aagtgtggcg tgaagccgac acatggtgga tggtagtcgg ggcgaaagat ccaggcaaca
      481 cggggcagat cctgctttat cgcggcagtt cattgcgtga atgggccttc gatcgcgtac
      541 tggcccacgc tgatgcgggt gaaagctata tgtgggaatg tccggacttt ttcagccttg
      601 gcgatcagca ttatctgatg ttttccccgc agggaatgaa tgccgaggga tacagttacc
      661 gaaatcgctt tcaaagtggc gtaatacccg gaatgtggtc gccaggacga ctttttgcac
      721 aatccgggca ttttactgaa cttgataacg ggcatgactt ttatgcacca caaagctttt
      781 tagcgaagga tggtcggcgt attgttatcg gctggatgga tatgtgggaa tcgccaatgc
      841 cctcaaaacg tgaaggatgg gcaggctgca tgacgctggc gcgcgagcta tcagagagca
      901 atggcaaact tctacaacgc ccggttcacg aagctgagtc gttacgccag cagcatcaat
      961 ctgtctctcc ccgcagaatc agcaataaat atgttttgca ggaaaacgcg caagcagttg
     1021 agattcagtt gcagtgggcg ctgaagaaca gtgatgccga acattacgga ttacagctcg
     1081 gcactggaat gcggctgtat attgataacc aatctgagcg acttattttg tggcggtatt
     1141 acccacacga gaatttagac ggctaccgta gtattcccct cccgcagcgt gacacgctcg
     1201 ccctaaggat atttatcgat acatcatccg tggaagtatt tattaacgac ggggaagcgg
     1261 tgatgagtag tcgaatctat ccgcagccag aagaacggga actgtcgctt tatgcctccc
     1321 acggagtggc tgtgctgcaa catggagcac tctggctact gggttaacat aatatcaggt
     1381 ggaacaacgg atcaacagcg ggcaagggat ccgcgtcact cttccccctt cacgaccttc
     1441 aataatatgc aatgcagctt cccgcccgat aatgtcatgt ggaagctgaa ttgtggtcag
     1501 cggcggtaaa aacagatgcc cgacgccaac cagattatca aagcccatta cagcgacatc
     1561 ctgcgggata cgtaccccct tcgccaaaag aacctgataa gccacaaagg ctgtgcgatc
     1621 gttaccacat atcagaacat caaaatctgg tttgcccgat ttgaagtgag cattgagtaa
     1681 acttgcgaga tcggtgtagt gatcatcacc tgttgccatg tgaaattgtt tcacctcagc
     1741 cagatctcgt ccagcatcac gccaagcctg ctcaaatccc tgccgacgat accctgttgc
     1801 caacgcactt tccggtagcc agaagcataa tggttgacga tagcccgccg cgagcaaatg
     1861 ctgtgttgat tcatattgtg cagtgtaatc atcagggata taactgggta acgctgggtc
     1921 atccgccaca cagttcgcca atacaatatt ttcaccatac agagactcag gcagcgtgat
     1981 atgtcgcagc cccattgtag tatagataat gccatccgga cggtgggcaa gcagctgacg
     2041 tgccgcgcgg gcagcgtcat cttcagaaaa aatattgatt aaaaaactat tccagccgaa
     2101 ctcgctggcg gtttgctcaa tggcaagcag aatatcaaca gagaaaggag tggtagccgt
     2161 gtcctgcgcc agcacggcga gagtcgacgg cttacgtcct tgagcgcgca tcttacgggc
     2221 ggaaagatca ggaacataat tcagggtctg gattgcctgc aatacgcggt cacgcgttgc
     2281 aggacgcaca gattctgcat tatgcatcac ccgggagact gtcatcatcg acactcccgc
     2341 caggcgtgcg acatccttta atgaagccat acccaagccg tttgccgtaa aacgggcact
     2401 gtagcagaaa cagacgtcac tggcgagatc caacgcccta tcacctgaca cagcaataca
     2461 ataaaaaata acaataattc ccggacaatt gtccccaatt ccgcctctgt tctcgccaac
     2521 gagtaaccat ataggcaagt aagtaaaaca tgccaactgg catcgccaac ctggagaaga
     2581 aaatataaat atggatcatt gtgctgccac tgtatcccga catcagcccg gaaattattg
     2641 cgattgctat cggttcaggt gcaattggct gcacgatcgt tacggactcg cttttctggc
     2701 tggtgaagca atattgcggc gctacgctca atgaaacatt taaatactat acgacagcga
     2761 catttatcgc ttcagtcgtc gctctggcgg gcacattcct gctgtcattt atcatctaag
     2821 cgcaaagaga cgtactatgg aaaacgctaa aatgaactcg ctcatcgccc agtatccgtt
     2881 ggtaaaggat ctggttgctc ttaaagaaac cacctggttt aatcctagca cgacctcatt
     2941 ggctgaaggt ttaccttatg ttggcctgac cgaacaggat gttcaggacg cccatgcgcg
     3001 cttatcccgt tttgcaccct atctggcaaa agcatttgct gaaacagctg ccactggggg
     3061 gattattgaa tcagaactgg ttgccattcc ggctatgcaa aaacggctgg aaaaggaata
     3121 tcaacaaccg atcagcgggc aactcctact gaaaaaagat agccatttgc ccatttccgg
     3181 ctctataaaa gcacgcggcg ggatttatga agtcctggca catgcagaaa aactggctct
     3241 ggaagcgggg ttgctgacgc ttgatgatga ctacagcaaa ctgctttctc cggagtttaa
     3301 acagttcttt agccaataca gcattgctgt gggctcaacc ggaaatctgg ggttatcaat
     3361 cggcattatg agcgcccgca ttggctttaa ggtgacagtt catatgtctg ctgatgcccg
     3421 ggcatggaaa aaagcgaaac tgcgcagcca tggcgttacg gtcgtggaat atgagcaaga
     3481 ttatggtgtt gccgtcgagg aaggacgtaa agcagcgcag tctgacccga actgtttctt
     3541 tattgatgac gaaaattccc gcacgttgtt ccttgggtat tccgttgctg gccagcgtct
     3601 taaagcgcaa tttgcccagc aaggccgtat cgtcgatgct gataaccctc tgtttgtcta
     3661 tctgccgtgt ggtgttggcg gtggtcctgg tggcgtcgca ttcgggctta aactggcgtt
     3721 tggcgatcat gttcactgct tttttgccga accaacgcac tccccttgta tgttgttagg
     3781 cgtccataca ggattacacg atcagatttc tgttcaggat attggtatcg acaaccttac
     3841 cgcagcggat ggccttgcag ttggtcgcgc atcatgcttt gtcgggcggg caatggagcg
     3901 tctgctggat ggcttctata cccttagcga tcaaaccatg tatgacatgc ttggctggct
     3961 ggcgcaggaa gaaggtattc gtcttgaacc ttcagcactg gcgggtatgg ccggacctca
     4021 gcgcgtgtgt gcatcagtaa gttaccaaca gatgcacggt ttcagcgcag aacaattgcg
     4081 taatgccact catctggtgt gggcgacggg aggtggaatg gtgccggaag aagagatgaa
     4141 tcaatatctg gcaaaaggcc gttaataacg tttcaacgca acatcgcaat cctttccctg
     4201 ggtgagcgat gctgccgatg gcgcagactt aagatccccg gtcttacccg ctataacccc
     4261 ctccttttat catcacccaa cgcctttcgc tgtaaacggc ggtttcgcaa accaaaccaa
     4321 aaccgtaagt aagataaaag caatagccgc catacggaaa atctcatttg cagaaataga
     4381 aagtgactgc tgggttattt cattattaat ttcattaaga actcctgaaa gcgaaccata
     4441 gtatttatcc ataatttgtg acgatgaatt aaacacgggg ttaaattgat cgatggttgc
     4501 tgtcaactga ctatggtgta acgattcgcg tcgtccccac agcgtcattg tcaacgacgt
     4561 accaactgat cctgacaagg tacgaaaaaa attactcata ctcgaggcat tggcaaattt
     4621 attatctggc aagcctgaaa acgaaatcgt tgttaagggt aaaaagaaac aggcaacggc
     4681 gaatccctgg aaaaactgtg gcaaaatgat gcctgtaaaa tcaatcgttg gcataaatgt
     4741 cacagaacgc cagtaatagc aaaccgcata catcaaaaaa ctgaatgtca ctaacacccg
     4801 catgtctatt ttattgccat aacgtcctat caaaggtgaa atcaatagtg gcatgatgcc
     4861 gatgggcgca taagcaagcc cggcccatat cgcattatac cccatcgttt tctggagtaa
     4921 ctgcggcata aggacgatcg ctccagagta aaataaatac gcgcaggtga tactcacaat
     4981 accaatggtg aaattacggg acttaaacaa actgagatca agaatcgggt tctctgaggt
     5041 cgattcccaa atgactaaag agatcagaga aataactgat actactgtta atattattat
     5101 tgtactcgag ttgaaccaat ccagatcgcg ccctttatca agcataattt gcaagccacc
     5161 aacaccgagc actaacaggg tcagtcttgg tagattcatt tttaccggtg aagtttcagt
     5221 ttctcttcct ttaagtaagg ttaagcataa tgtcaggacg ataatcccca tagggacatt
     5281 gattaaaaat atccaacccc agctaaagtt atcgcaaata tagccgccca atatcggccc
     5341 acatatcgga gcgataatca cggtcattga ccataatgcc agagcaaatg ttcttttttc
     5401 tggtggataa ttccttaata ataaactctg tgacagtgga attaacggcc ccgccattaa
     5461 tccctgaacg actctaaaaa atatcagcac atcaagattg gtcgataaac tacacattaa
     5521 ggaagacagc gaaaaaaaag tgactgaaag taaaaataac cttaattcgc ctattctttg
     5581 tgccagcctg ccggtaacag ggatcgcaat ggcatttgct acgccaaacg aggtgataac
     5641 ccaggtgcct tcgtctgttg atgctcccag aaagccagat attgtcggta ttgcgacgtt
     5701 agaaatagtg gaatccaaca tttgcataaa cgtcgctaat gacaatgcaa tagtaacgca
     5761 ccataacgtc ccaccggtta atggtgccgg agttgattta gtgattgcca ttaaagttgt
     5821 ccattatgcg aaataatgtt gctaatttct ttttctatcg gactggtatc gataactaaa
     5881 gccttactgg tataagcagg catggaggtc acggttgaag ccagatcagg catctcagca
     5941 atgtcttcgt ccttcgtatc aatagttgca gtcatcgata aaccaatgcg caaggggtgt
     6001 tccatgagtt cttttggatc aagagaaact tcaaccggta cacgctgaac gattttgatc
     6061 cagttccctg tcgcattttg tgcaggtaat aaggagaacg cattgccggt tcccatattg
     6121 atccctgtca cccgaccatg aaacacaaca ttttcaccat aaagatcgct gataatattg
     6181 actgattgac caatccgtac atccgtgagt tgtgtttctt taaagttggc attaacccac
     6241 atttgacgtg ccggtacgac agccattaac gattgtccgg aactcactgt ttcgccgacc
     6301 tgaacacttc gctgggcaat atagccagta accggactcc ttatatccgt acgtttaagc
     6361 accaaccagg cttctttggt tgcatccgcc gcttcaacta cttgtggctg acggtttaat
     6421 ggtgtgttca ttactaaagc tttattcgct ttataagcct ggatagcggc attcagtgcc
     6481 gctttgctac ttattaacgt atctttggta tgctccagcg tttcttttga aataaccccc
     6541 tgcttcgcta aaggcacacg acggttataa tcttctaaag attgttgata ctgaatacgt
     6601 gctgaagcga cttcggcact gtattgttta tcctgtaagt acagtttatt cgtttgccga
     6661 acaatatttg ccagattatt tttagcttta ttgagtgcga tagtggcatc agttttatcc
     6721 agtgaaacta aaatgtcacc ctgtcgaacg tagttcgtat ctttatgatt aactacagtg
     6781 acactacctg agacttgtgc agaaattgga tctgcattcc ccgtgacata ggcgtcatct
     6841 gtactaatca tgtcttctaa ttccattgac caataggcat aggcacctga aaacgcaata
     6901 aataaaacta tcaccaataa agaaaagtat ttttttctgt tagactgttt tttatttgaa
     6961 ttaatctgtt ccactattat ctctcatttc gcatagatga aatttatgaa ttgaatgaaa
     7021 agagtattca ttcttcatat caaacacagt gcatgtgttg cacattatcc caggaacgtt
     7081 ggaggcctaa tttaaatgag gtgcaaaaaa aacaacagag gactaaaccg tggcttttgc
     7141 aatacaattc ttacgcctgt aggattagtg agaagactta tagtgctaac ttgaaactat
     7201 aaatcatcgg tataatccct gattttattg ttgacatttc atttatgccg actatttata
     7261 tggtatactt gtcgaattat cttaaaggaa actcagattt tcttattttt attgagaaaa
     7321 tgagatgacg ccttatgtct gtattactac agggagaagg gagatgcttc attgcaaagg
     7381 gaataatcta tgaacgcaat aattattgat gaccatcctc ttgctatcgc agcaattcgt
     7441 aatttattga tcaaaaacga tattgaaatc ttagcggagt taactgaagg cggaagcgcc
     7501 gttcagcggg tggaaacact taagcctgat atcgtcatca ttgatgtcga tatccccgga
     7561 gttaacggta tccaggtgtt agaaacgctg aggaagcgcc aatatagcgg aattattatt
     7621 atcgtctccg ctaaaaatga ccatttttac gggaaacatt gtgctgatgc tggcgctaat
     7681 ggtttcgtga gtaaaaaaga aggcatgaac aatatcattg cggctattga agctgcaaaa
     7741 aatggctact gctatttccc cttctctctc aaccggtttg ttggaagttt aacgtccgac
     7801 cagcaaaaac tcgactcctt atcgaaacaa gaaattagtg tcatgcggta tattcttgat
     7861 ggcaaggata ataatgacat tgcggaaaaa atgttcatca gcaacaaaac tgtcagcacg
     7921 tataaaagtc gcctgatgga aaaattagaa tgtaaatcac tgatggatct ttacacattc
     7981 gcgcaacgta acaaaatcgg ctaatcacat gaagttttta ccctattttt ttcttctctg
     8041 ttgtggtctt tggtcgacca taagtttcgc agacgaagat tacatcgaat atcgtggcat
     8101 cagtagtaac aaccgtgtca cacttgatcc agtacgtctg agcaacaagg aattacgttg
     8161 gttagcgagc aaaaaaaatc ttgtgattgc agtacacaag tcccaaacgg ctaccttgtt
     8221 gcataccgat tcgcagcaac aggttcgtgg tattaatgct gattatttaa atcttttaaa
     8281 aagagcgtta aatatcaaat taacactccg ggaatacgca gatcatcaaa aagcaatgga
     8341 cgcgcttgca gaaggtgaag tcgatatagt gttatcacat ttagttactt cgccgcctct
     8401 taataatgac attgctgcaa ccaaaccatt gataattacc tttccggcgc tggtaaccac
     8461 ccttcacgac tcaatgcgac cgcttacctc accaaaacca gtaaatattg ctcgggtagc
     8521 aaattacccc ccagacgagg taattcatca atcatttcca aaagcaacaa ttatctcttt
     8581 tacaaattta tatcaggcat tagcatccgt ctcagctggg cacaatgatt actttattgg
     8641 tagtaacatc attaccagca gtatgatttc ccgctatttc actcactcct taaatgtagt
     8701 gaaatattat aactcgccgc gtcaatataa ttttttcttg accagaaaag aatctgtcat
     8761 tcttaatgaa gtactcaata gatttgttga tgctttaaca aatgaagttc gctatgaagt
     8821 atcacaaaat tggcttgata caggaaacct ggcctttctg aacaaaccat tagaactcac
     8881 tgaacatgaa aaacagtgga ttaagcagca tcccaattta aaggtgctgg aaaatcctta
     8941 ctcgcccccc tattctatga cggatgaaaa tggctcggtt cggggcgtta tgggggacat
     9001 tcttaatatt attaccttgc aaacaggttt aaatttttct ccgatcaccg tttcacacaa
     9061 tatccatgct ggaacacaac ttagccccgg aggatgggat ataatacctg gcgctattta
     9121 tagtgaagat cgagaaaata atgttttatt tgctgaagcc ttcataacaa cgccttacgt
     9181 ttttgtcatg caaaaagcgc ctgacagtga acaaacatta aaaaaaggaa tgaaagttgc
     9241 cattccatat tattatgagc tgcattcgca attaaaagag atgtatccgg aggttgaatg
     9301 gatacaggtc gataatgcca gcgctgcatt tcacaagatt aaggaaggtg aacttgatgc
     9361 tctggtcgcg acacagctaa attcgcgtta catgatcgat cattactatc ctaatgaact
     9421 ttatcatttt cttattcctg gcgttccgaa tgcatcgctt tcgttcgctt ttcctcgcgg
     9481 agaaccggaa cttaaggata ttattaataa agcactgaat gcaattcccc caagcgaagt
     9541 tctgcgcctg acggaaaaat ggattaaaat gcccaatgtg accattgaca catgggacct
     9601 atatagcgag caattttata ttgttacgac attatccgtt ttattagttg gcagtagcct
     9661 tttatgggga ttctacctgt tacgctcagt tcgtcgtcgt aaagtcattc agggtgattt
     9721 agaaaaccaa atatcattcc gaaaagcact ctcggattcc ttaccgaatc caacttatgt
     9781 tgtaaactgg caaggtaatg tcattagtca taatagtgct tttgaacatt atttcactgc
     9841 ggattactac aaaaatgcaa tgttaccatt agaaaacagt gattcaccct ttaaagatgt
     9901 tttttctaat gcgcatgaag tcacagcaga aacgaaagaa aatcgaacaa tatacacaca
     9961 ggtatttgaa attgataatg gcatcgagaa aagatgcatt aatcactggc atacattatg
    10021 caatcttcct gcaagtgaca atgcagtata tatttgtggt tggcaagata ttactgaaac
    10081 gcgtgatcta attaatgcac tcgaggtaga aaaaaataaa gcgataaagg ctaccgtagc
    10141 aaaaagtcag tttctggcaa cgatgagtca cgaaataaga acaccaataa gctctattat
    10201 gggcttcctg gaacttctgt cgggttctgg tcttagcaag gagcaacggg tggaggcgat
    10261 ttcacttgcc tacgccaccg gacaatcact cctcggctta attggtgaaa tccttgatgt
    10321 cgacaaaatt gaatcgggta actatcaact tcaaccacaa tgggtcgata tccctacttt
    10381 agtccagaac acttgtcact ctttcggtgc gattgctgca agcaaatcga tcgcattaag
    10441 ttgcagcagt acatttcctg atcattacct ggttaagatc gaccctcagg cgtttaagca
    10501 ggtcttatca aatttactga gtaatgctct caaatttacc accgaggggg cagtaaaaat
    10561 tacgacctcc ctgggtcaca ttgatgacaa ccacgctgtt atcaaaatga cgattatgga
    10621 ttctggaagt ggattatcgc aggaagaaca acaacaactg tttaagcgct acagccaaac
    10681 aagtgcaggt cgtcagcaaa caggttctgg tttaggctta atgatctgca aagaattaat
    10741 taaaaatatg cagggcgatt tatcattaga aagtcatcca ggcataggaa caacatttac
    10801 gatcacaatc ccggtagaaa ttagccagca agtggcgact gtcgaggcaa aagcagaaca
    10861 acccatcaca ctacctgaaa agttgagcat attaatcgcg gatgatcatc cgaccaacag
    10921 gctattactc aaacgccagc taaatctatt aggatatgat gttgatgaag ccactgatgg
    10981 tgtgcaagcg ctacacaaag tcagtatgca acattatgat ctgcttatta ctgacgttaa
    11041 tatgccgaat atggatggtt ttgagttgac tcgcaaactc cgtgagcaaa attcttcctt
    11101 acccatctgg gggcttacag ccaacgcaca ggctaacgaa cgtgaaaaag ggttaagttg
    11161 cggcatgaac ttatgtttgt tcaaaccgtt gaccctggat gtactgaaaa cacatttaag
    11221 tcagttacac caggttgcgc atattgcacc tcagtatcgc caccttgata tcgaagccct
    11281 gaaaaataat acggcgaatg atctacaact gatgcaggag attctcatga ctttccagca
    11341 tgaaacgcat aaagatctac ccgctgcgtt tcatgcacta gaagctggcg ataacagaac
    11401 tttccatcag tgtattcatc gcatccacgg tgcggctaac atcctgaatt tgcaaaagtt
    11461 gattaatatt agccatcagt tagaaataac acctgcttca gatgacagta agcctgaaat
    11521 tcttcagctg ctgaactctg ttaaagaaca cattgcagag ctggaccagg agattgctgt
    11581 tttctgtcag aaaaatgact aaattgcggc tcccacaatg ttctaatgtg ggagctattt
    11641 accagcacat cttacattta tgatgagaac tcctggcgaa tttgttcccc atgctggtcg
    11701 agcgttgccg ctcccggcat aacatgcggg tccgcgcagc cgctgatttt tatcggatta
    11761 cccggcatca ttattcctcc ggcttcaatc aacatatttc tcgcctgagt ttgtggcaaa
    11821 ttaatggcct cagccacact taataacggc gcgacgggca caccaacttc atgtattttg
    11881 gctaaccaaa cttcagctgc ctgcgttttt aacgttcgct caatatattg tttaagaata
    11941 gcctggtttt gtacgcgtaa aatattgctg ctaaatcggg gatcattaac cagttccgta
    12001 agctccagtg cctggcataa cgcagaaaaa agcttgtcat taccacaaca aatcgtaatc
    12061 ggcttatcct gagtatcgaa aacatcaaaa ggtgccatgt agggatggcg atttcccagg
    12121 cgttgtggtg acttccctgt cgcaatatat gccatcagac catgctccag aaaactcagc
    12181 gtggcatcaa acatcgctat atcgacatgc gcccctctct ggctcttttc gcggccataa
    12241 agtgcactca ctattccgct gaataaatag acaccgccgc atagatccgc aagagaggta
    12301 ccaacacgca ctggcggagc atcagggtac cctgtttcca tcattatccc actcattgcc
    12361 tgaatgatgg tatcgtaggc aggagcatct tttagcggac cggtatgtcc gaaacctgac
    12421 gatgaagcat atatgaggcg cgggttgatt tcttgtaacc tttcccatga aaaccccagt
    12481 ttttccattg tacctaggcg aaaattctca gctaatacat cagcttgttt gagcatattt
    12541 ataaatatac ttttatcgtg atcattcttt aaatcaagaa ccacactctc tttgccatga
    12601 ttaataaaac tgtaatagag tgactgtcca tccacatagg gaccaaatgt gcgggtatca
    12661 tcaccatgac ctggtggctc aactttaatt acccttgcgc ccatattata aagaagttga
    12721 gttccgaaag gtccattaag gacatgtgtc atatcgataa ctaataagcc ttcaaacggc
    12781 cctttgcttt cattatttgt catttgctta tgccttgaaa aaagccgaag gtgaaaagac
    12841 acttcggcat ttatgtaaaa tcaataacat gattaattat gaaaccagtc gactgacata
    12901 aatccacaag ggagccgtga cgacaaagcc cagtacgctc acagccaatg acgctgtacc
    12961 agtacgggtg tagacattaa accggctggc aataatgatt ccggagaatg ccggcggtaa
    13021 tgcgcctgcc agtaccatca tctgcaaatg ttcgctgttc aaatgacatg ccataccaac
    13081 gagaagcagc gccagtggca tcagaatcaa cttcaggaag gtgttataag caatttcggc
    13141 actgaactcg aatttatgtg cagccagagt caaccccgca gcgaataccg ctacccctga
    13201 gttagccttc gcaatcagat taaaggttgg gtcccatgct gccggaattt ttaccccaac
    13261 cagcaccagg atcgttgcca gaacaggtgc ccatactact ggctcctttg ccgcagaaat
    13321 taatgcgctc agattactat tcttcttacc atccgctcct gaggaaggat tcagcaaata
    13381 cagaccaata ggaatagtaa ttgcgttaac aataatagaa ataattgcta ccactaaacc
    13441 tgttgatacg gaatcaccat aaataggatc gagaactgca aaccccagga atccaatggt
    13501 aggtgaaccc gcaattaatg cacatacagc tgcttctgca tgggtacgtt taaaaaattt
    13561 gtagcaacca aaccaggaga aaaagaaaca tccgacaata accacaagtg atacaagggt
    13621 cagacgagtg tccgcaaaaa tcatttccct gtttgcccgg gtaatagaaa caaatagagc
    13681 cgcaggaaga gcgtagttta ataccagttt attaaatgcg cgggcttgat cttctgaaaa
    13741 agtttctcgt ctgccgctaa aataacccaa cagcatgatt acgataatag gcaataaatc
    13801 gccaataaaa aatgttagca taatagtatt tccgataagg gtcccctcac catcgcataa
    13861 tgagttggca agaggagacg atgtcagtat ttaattacca gcgacttgtt ttgggttaag
    13921 tttggtaata tggccacttt cagttcctgc tgcagggtcg ataaccacat taataatggt
    13981 cggtttgcgc gactgaatac cggtggttaa agcatgacga agttcatctg tcgtggtgac
    14041 gttatagcca acgccacgaa acgcatccat taatttgtca taccttgcat ggtgcaacag
    14101 atccgttggt gatggtgcac cagcgccact gagatcaaca ccgtctcctc tgtagatgcc
    14161 gccattatta aaaataacga tcgtcaccgg caggttatat cgacaaatcg tttcaatttc
    14221 catcccactg aaaccaaaag cactatcacc ttcaatggcg acaaccggag aaccagaagt
    14281 cacgctagca ccgatggcat agcccatacc gatgcccatg acaccccagg taccacaatc
    14341 cagacgacga cgtggtttat acatatcaat aatatttcgt gcattatcca gggtatttgc
    14401 accttcatta actaaataaa tatcctggtt ctcgcgcaat acgtcgcgca cagcacttaa
    14461 tgcattaaag taatttaatg gttgagtatc tgtacttaat ttttcatgca ttttttgtgc
    14521 attttgctgc ttgtggatat ttaaaatatc gcgccatacc agtggagtcg taaatgtgtt
    14581 ttgtttcagt tctgccagca taccttgcat actggatgca atatcaccaa cgactggcac
    14641 agcaatgggg cggttgctgt caatttcctg cggttcaata tccagttgaa taaactgtgt
    14701 atctgccgcc catccttttt taccgtgtgc caataaccaa ttcagtcgtg caccaacaag
    14761 catgacaacg tcagcatttg ccagggcaaa cgaacgcgca gctgccgcag aaagtggatg
    14821 cgtatcttca aggatccctt tcgccataga cattggcagg aatggaatct gagcactttc
    14881 aataaattca cgaagctgtt catcagcttg tgaatacgcc gcgcctttgc caaggataat
    14941 taatggccgt tcagcttttg ctaaaagcga aattgcgcta gtgactgact tcgggcatgg
    15001 caataatgct ggcgacggat tttcaacttt aacaatcgtg gttaacgctt cgtctttttc
    15061 catcgtcgcg gccaggacat ttgctggcaa atcaagataa actccgccag ggcgacccga
    15121 tacagagacc cggatagcgc gtgccaatgc aatgccaaga tcctgcggct gattaacgcg
    15181 aaatgctgct ttggcatacg gttttgccgc attcatttgg tccagctctt cataatcacc
    15241 ttgctgtagg tcgacgatcg cgcggtcgct ggagccgcta atcatgataa tcggaaaacc
    15301 atttaccgtt gcgttggcca atgcggtcaa accattgagg aatcctggcg cagaaactgt
    15361 caggcagatc cccggttttt gggtaagaaa accgcttgcc gcagcggcat agcctgccga
    15421 ctgctcatga cgaaaaccaa tataacgaat gccttccgcc tgggcatggc gtgccatatc
    15481 cgtcacagga atacctacaa caccataaat agtgtcaata ttattctgtt ttaatgcttc
    15541 aacgatgata tgcataccat ctgtcatttg aagttgatct gacatgatta ataacctcga
    15601 aatttttata aattgggagc cagaagcccc cgttaatatc agatggcgtg gttttgcttc
    15661 attgcagcaa tttcatcgtc gctataaccc agctcctgca atacagcagc ggtatgttca
    15721 cctaatagcg gcgcagcttt aatatccggc gtaaaggcag agaatttcat tggacaacca
    15781 acggtcagat attttccacg caacggttgt tccacttcaa caacactgcc actttggcgc
    15841 aaagagggat caagtgaaat ttctttcata cttaaaaccg gtgcacaagg aatatcaaac
    15901 tgagtcaaat aggccaccgc ttcatgttta tcaatagtga cagtgtattt ttcgatttca
    15961 gcaaaaatat cgaaaatatg tggctgtcgg gcatgggctg tactgtatgc cggatcggta
    16021 atccattctg gtttgccgat ggctttacag gtgttttccc agttttgctc ctgaatagtg
    16081 aaataaatat aggcgttagg atcggtttcc cagcctttac atttcaggat ccagccaggc
    16141 tgaccgccac cacctgcatt accaccgcgg ggaactgcat caccaaatgt accattcgga
    16201 tactgcgggt attcttccag ataacccaat ttatcgagac gctgctggtc acgtaatttc
    16261 acgcggcaaa ggttcaatac ggcatcctgc attgacatgg tgactcgttg cccacgcccc
    16321 gttttttcgc gatgcagcaa agcagcaagt aaaccgatca gcaaatgcat tccggtgttg
    16381 ctgtcaccca acgctgcagc gcttaccagc ggcggaccat cccaaaaacc cgtagtggat
    16441 gccgcgccac ccgctgcctg agcaacgttt tcataggctt ttacattcac ataaggcgaa
    16501 cactcatcaa accctttgat cgaaccaaaa atcagacgtg gattgatttc ttgaatatgc
    16561 tcccaggtga agcccatgtg atcaatggcc cctggatgaa agttctcgac taagatatca
    16621 gcttcgcgga tcagcttttc cattacctct ttgccttccg ctgttttggt atttaactca
    16681 atagaacgtt tgttactgtt aagcatggtg aagtaaagcg catcgatatc aggaatatct
    16741 cgcagttggt gacgcgttac gtcaccaacg ccgggacgtt caattttaat aacgtcagcg
    16801 ccaaaccagg ccagcatttg agtacaagat gggccagatt gcacaccggt gaaatcgaga
    16861 actttaattc cttgaagtgg agttgacata gcaataccta ttagttaatt aaatatgtgt
    16921 taatttagcg tttatttaag gcataacgcc ctctataaat aatctgcaaa atatcttcgt
    16981 agcggcactc gatattttta aattcctgta tcaagtactg aaaattattt tatcgccact
    17041 tccaattcct ttctgcatgg taagtagtga ggacgaaaag aagaatatga taaatattaa
    17101 ttcgtggtaa tcagagagag tcgcgacatt tgtgatgtta ttcttacaac tgtaagaagc
    17161 gccggtgatt tacaagtaag agtatgatta cgctttttaa agcgtaccat aaagcataac
    17221 ataataaatg atttgtgatg acacgggatt attgcggttt actttgagga aaataaaatg
    17281 cgtgtttata gaaccgcgag cgccaaataa ttttatgacc acaacctgtc gtattgtggt
    17341 cataaaaaat tgtcggctgg agggagttaa atcctttacc tcaatatact gcctgtcagc
    17401 attagttcac aaacagtgct tcgctatcga cgatgattcc atcttccgca cctttaagcg
    17461 ccaggtttgc ctcgtagtat tgctttttat caagcagttt ctgcgcatca gcaagtgcat
    17521 tacgagtctg tttaagcggc atcagatatt ggttttccat tacgccaaca ccggccaggc
    17581 gaagctcttc cattgcgccc tttttatccc ctttcgccat tttttcattg gcaattttta
    17641 tcgcggcttc tttttcgggt gttgctacat aactctcaga aattccgaca gatgcattaa
    17701 tgacaatata ttggtcatca ttgagattgg tctttttacc tggcttagcg aactttgccc
    17761 attcggtact gtcatctgac agcaaagcgg aagcttcatt ggtcagttct tttgcctttt
    17821 ccggatcgcc atgaaacaga gccaggcgcg ctacctgcac gtcacgcatc gcatataaac
    17881 cttgttcaga gatacgctct gctgctgccg ttttctgggt ttgctgagtt tgctgttgtg
    17941 ccgctaccgg tgcattatca gcagcccata cagttgaaga tgccagaatt gctgtgacca
    18001 tcgtagccat aattaaacgt ttcatactta cctcattctt taacagtttt agaaaacgcc
    18061 gccatcatat tgggtgagca tgaaatgaga aatcagagag caacggtcat tttgaaaggg
    18121 ttatcttaca attgtagtta ttatcgcaaa gcggcattta gtcttacagt tgtaggaata
    18181 tgcctcctgt cgcagagaac tattcttata taccacaaag agatgtcatt tattatattg
    18241 atgtaaatat tgattaatta tatgtaaata cagccataac atcgtatggt tctcagtgaa
    18301 ccattttcag gataactttc tatgaaagta aacttaatac ttttcagctt atttttattg
    18361 gtctctatta tggcatgcaa tgtttttgca ttttccattt ctggtggtgt aagtgagagg
    18421 agctataaag agactgaaaa aacatcagcg atgacgacca cacactctac aaaacttcag
    18481 ccatcacagg cgattttgtt taagatgaga gaagatgtgc caccattaaa cctcacagaa
    18541 gaaatcacgc ctacttatcc gacaaaggcg aattatctta ttcatcctgt gcgatagcgt
    18601 ctgtcacatg gcaggtgaac aatcatgtct gatgctgatg tgaacacctg cccaaatgag
    18661 ccatcctgac ccactatcgc ctgccatacc ccaccataaa gcgaatcatc aagccacaag
    18721 ccggggcaag aaacataaat atccccgtca ccagttcatt accgccagtt aaccacatgg
    18781 tgaagtaata aatgagtgca gcgaggatca ccatgccgaa gaaagaagag accgccgata
    18841 ccacgttcag tacctgaccg atgtagccgc ttacgcagac aatacacagg gcgagaaaca
    18901 tccataaata aatcatagct caacctcctg ccgcgctaaa aaacctgtaa cttcagtcac
    18961 tttaatagta agacaatcct taacctctgg caatttttgc taagcatcaa tgaaaacagc
    19021 acgttaaatg caacgctatt gcgtgcggaa aattttttac tttgcacgat taataatcag
    19081 tagctgaaag cagtcagcca gtgataaagt agagccaccg cattcatgct cctcgtggtt
    19141 atgtcctgac tagtctttac actctttaca ggaaccattg tcgtacatga tggcccaacc
    19201 aattgagtgt accgctgtcg acatggaatc ttctgcgata caacaattcg tatctacaga
    19261 aggtaactat gtttccacaa tgcaaatttt cccgcgagtt tctacatcct cgctactggc
    19321 tcacatggtt tgggcttggt gtactctggc tttgggtaca gcttccttat cctgttctct
    19381 gctttctcgg cacgcgtatt ggcgcaatgg cgcgaccatt cctgaaacgt cgtgaatcta
    19441 tcgcccgtaa aaacctggaa ctttgtttcc cgcagcattc tgcggaagaa cgcgagaaga
    19501 tgattgccga aaactttcgt tcactcggca tggcgctggt agaaaccggc atggcatggt
    19561 tctggcccga cagtcgcgta cgtaaatggt ttgatgttga agggttggat aaccttaaac
    19621 gcgcacaaat gcaaaatcgc ggcgtaatgg ttgtcggcgt ccattttatg tcgctggaac
    19681 tgggcggccg cgtgatggga ctgtgccaac caatgatggc tacctatcgt ccacataata
    19741 atcagctgat ggaatgggtg cagacccgtg ggcgcatgcg ctctaacaaa gcgatgatcg
    19801 gcagaaataa tctgcgcggc attgtcggtg cactgaagaa aggtgaagcg gtatggtttg
    19861 ctcccgatca ggattatggt cgtaaaggca gctccttcgc gccgttcttt gcggtggaaa
    19921 atgtcgccac aaccaatggc acctatgttc tctcccgtct ctctggcgca gccatgttga
    19981 ccgtaacgat ggtaagaaaa gcggattaca gcggatatcg tttgttcatc accccagaga
    20041 tggaaggcta cccgacagat gaaaatcaag ccgctgctta tatgaacaag attatcgaga
    20101 aagagatcat gcgcgcaccg gagcagtacc tctggatcca ccgtcgcttt aaaacgcgcc
    20161 cggtgggaga atcgtcgttg tacatttaac aaactgctct tactctttcc ctcctcaaat
    20221 cggccataat aacctcatga tttttatagc ttttttctta agttttttcc ttcgctgaat
    20281 atctaattat taccgtcaga aattaaactg tcgccgatat gagacacttg taagttacta
    20341 aaagtaaata agaaaatgcc tcttgtctcg tttcagtttt aggcgtacat tagcgccgtc
    20401 tcgagcagtt tagctcagaa ttctggggtg atgcccacgg taaaatacag ggcttttaag
    20461 gtcgctttgc ccggtgtcaa ctcactgtat caggtgtaat gaagtcattc aggcgtaaca
    20521 gtaattacgc ggagagatgt aaagtgaaat atttctttat gggcatttct tttatggtca
    20581 tcgtttgggc cggtactttt gccctgatga tctaaaagca aactgtgcaa tgaaaaacag
    20641 gagccatcag gctcctgttt gcatttctga cgacggtgtt tattccgcgt tttcgtgaat
    20701 atgtttgctg ctgacgggta gtaaaccgtc ggcacggaac atcgctttaa tccctctgat
    20761 ggcctgacga atacggtcgt ggttttcaat cagtgcaaag cgaacatgcg tatcaccata
    20821 gtcgccaaag ccaatccccg gcgagacaca aaccttcgct tcgttaagga gcttcttggc
    20881 aaattccagc gatcccatgg ccgcatatgg ttccgggatt ttcgcccaga catacatcga
    20941 agccttcggc atttcgacca tccagcccgc ttcatgcagc cctttaacca atacatcacg
    21001 acggcgtttg tactgttcgg caatgtcgcg cacgcactgt tgatcgccct cgagtgccgc
    21061 aatcgccgcc acctgcaacg gcgtaaaagt gccgtaatcg tgatagcttt tgattcgcgc
    21121 cagggcactg accagcgttt tgttgccgac cataaagccg atacgccagc ccgccatgtt
    21181 gtagcttttc gacagcgtaa agaactcgac cgccacatcg cgtgcacccg gcacctgcat
    21241 aattgacggc gctttccagc catcgtagac gatatcggca taggccaggt catggaccac
    21301 cagcacatcg tagcatttcg ccagcgccac aaccttttcg aagaactcca gttccacgca
    21361 ttgcgcggtt gggttcgacg ggaagccgag gatcatcatc ttcggtttcg gataactttc
    21421 acgaatggcg cgctccagtt cgttgaagaa atcgacacct tccaccagcg gcactgagcg
    21481 tacctgcgcc ccggcaatca ccgcgccata aatatggatc gggtaacttg gattcggcac
    21541 cagcaccgtg tcaccatgat ccagcgtcgc cagcatcaga tgcgccaggc cctctttcga
    21601 accaatagtg acgatggctt ctgattccgg gtcgatttca acgtcgtagc gatcctgata
    21661 ccagcgggaa atggcgcgac gtaaccgcgg aatgccgcgt gaagtggagt aaccatgcgt
    21721 gtccgggcgc tgggccacag tacataattt ttcgacgata tgcggcggag tcgcaccgtc
    21781 cgggttaccc atgctgaaat cgataatatc ttcgccgcgc cgacgcgcag ccattttcag
    21841 ttcagcggtg atgttaaaaa cgtagggcgg gagacgatca atgcgcgtaa agcgacgttc
    21901 agggcgagtg tcagccataa tttcctcaga ttaacgttag cgcccggacc gtccgagcga
    21961 cgctgccaca atggtggctg ttttgaaaat agcctgatta atttctgtct gtccagcgtt
    22021 ttagaacatt tttatcaatg gatttaaatg aggaactcca cttcaaaaac aggaaatttt
    22081 tctacgctaa caagaaaatc accaccaatt ttattaacat tataatttca ataaatttac
    22141 cagccagaag tgcattatca agagatacat tgctaataca ccatttatct tttaatcccc
    22201 ttttaaattt atggtttttc ctcaaaaacg gcctgctggt catcagcctt caggttacct
    22261 atcatagagg tttaatcctt attcagagtc acccgtgcac gaaatattca acatgctgct
    22321 ggcggtcttc gatcgggcag cgttaatgct tatctgcctg ttctttctca tccgtatccg
    22381 cctgtttcgc gaactgttgc acaagtcggc gcactcccca aaagaattgc tcgccgttac
    22441 cgccattttc tcgctgttcg ccctgttcag cacctggtcc ggcgttcccg tagtcagcac
    22501 ctggtccggc gttcccgtag aaggctcgct ggtgaacgta cgtattatcg cggtgatgtc
    22561 cggcgggatt ctgtttggcc cgtgggtagg catcattacc ggcgtgattg cgggtattca
    22621 ccggtattta attgatatcg gcggcgtgac ggcgatcccc tgctttatca ccagcattct
    22681 ggcgggttgt atatcaggct ggataaacct gaaaatcccc aaagcacagc gctggcgctg
    22741 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    22801 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    22861 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    22921 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    22981 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    23041 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    23101 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    23161 cagctacgtg aaatggcaaa taaggcagag attcgcgccc tgcaaagcaa aattaatccc
    23221 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    23281 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    23341 gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag
    23401 caggcccgct ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc
    23461 tgcattccca gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag
    23521 ccttgcaaag gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc
    23581 attgcggtgc gagataccgg gcacggcatc gttccaaagg tgattgagcg ggtcgaagcg
    23641 aatgaaatgc cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg
    23701 tatggcgagg ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt
    23761 cctaaccaac gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata
    23821 ttgtgaaagt catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa
    23881 ttaaagagca cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga
    23941 agtttttgca gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg
    24001 atggcgtgtt gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca
    24061 tcaccgcgtg gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc
    24121 tcaaaccgta tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc
    24181 aacaacagca gaccagcagt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24241 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24301 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24361 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24421 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24481 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    24541 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    24601 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    24661 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    24721 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    24781 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    24841 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    24901 gccgcaccac gtcgttgttg catcctgctc gccagaccca gcttcccagc ctgcaacgcg
    24961 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25021 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25081 cgccgggtaa ttcacaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttt
    25141 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25201 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25261 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25321 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25381 agtttagtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25441 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25501 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    25561 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    25621 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    25681 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    25741 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    25801 acaggaactg gatcgcctgc tctttattgc taaaatcctg atcaacaaag atattctcca
    25861 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    25921 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    25981 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26041 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26101 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26161 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26221 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26281 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26341 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26401 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26461 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    26521 ccgcgcgata gccgaggaac gggttctctt cctggggaat attcagataa ggaatgcttt
    26581 tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca
    26641 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
    26701 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
    26761 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
    26821 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
    26881 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
    26941 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
    27001 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
    27061 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
    27121 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
    27181 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
    27241 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
    27301 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
    27361 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
    27421 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
    27481 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
    27541 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
    27601 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
    27661 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
    27721 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
    27781 agcttttagc atcagccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
    27841 actggctgca ctgttcttta agttcccacg ccggacgagc atgtagaccg ttaggcaaag
    27901 gacagagaaa ttgaatcgtt aacataaccg ctccgggtta atcaggagat catctgaaat
    27961 ccgtcagttg aacaaccgtc tcacgcgtaa gacgttgaat aagggcagat aaaagttgct
    28021 ccatctgcaa aatgtcgcgg caatcggcaa tcgatgccgc gcaatgtccg tggcgggtgg
    28081 caggccccat taccaatgtg ggtacgccag taccggttaa gtgcaccgct ccaccgtccg
    28141 tgccgccgtt actgaacata tccgcctgca acggaacgcc aatttctgcc gccacggttt
    28201 caatccacgc ggtaagtttt ggcggcgcaa tcagcgattt atcgcttaac accagcatcg
    28261 gcccgttacc aatctgacga tggttagccg cgccataatc aaagtttttc gcccagcagg
    28321 cggtatcaag cacaatggcg acgtccggcg acaccgcgcg ggtagcagtt tgcccgccgc
    28381 gtaatcccac ctcttcgctg gaacttgcca ccagccacac ttccgcaggc agttcggcgt
    28441 cgtgcagctc acgtagtaac gtcaccagca gatagcaact gaggcgatca tcaaaggctt
    28501 tgcccatcac ccgctggtga gggaggacct gaaaagtggt atcgaaggtg acgcgatcgc
    28561 ctggacgaat tcccgcctgc atcacttcgt cataggtgcg cgcgccaatg tccacgcgca
    28621 tggcgctaac gtcattcccc tgccggtcgc cgtcaagcag gcctggaatt ttgcactctt
    28681 cacgggtggt gatgcgcacc ggctgcagct ggcgggcagc catgcgcacg ttgccaaccg
    28741 gcagcacgtc aatagcgcct tcgcgggaga tgctgcgcac cataaatccc acttcgtcca
    28801 tatgggcgca gatcatcacc ttcggacctg tcgattcatt gaggcggatc agcaccgatc
    28861 ccagaccatc aaatcgcact tctttttgca ggcgagccgc ttcttccagc aggatctgcc
    28921 gcacttcctg ttccgaggag gcgatcgcat ctgcctcgct caacgctttt aatagcgata
    28981 aatccattat gcctctcccg tgagcaacac tgttttcggc atggcgtaga gcacttctgc
    29041 gccttgcggg gtaaccagca caacatcttc gatgcgcacg cccccttgcc ctagcaaata
    29101 aatccccggc tccacggtca gcaacatgcc tggctgtagc gtcgtggtgt cccgcggtga
    29161 aaaacgcgga tcttcatgga cttcaatgcc gatagcgtga ccagtgttat gaccgaaata
    29221 gtcgccaaat ccagcctcgg taatgacccg gcgcgcggcg tcgtcaatct gctggcagcg
    29281 cacgccgggg cgaattgcag agattgccgc gagctgtgcc tgcaggacaa tctgatagac
    29341 gtcaaacagc gggtgagatt cggcgctcac cccttcgcca ttcaccagca aggtgcgcgt
    29401 catatcagca gtagccctga tacagcgcac caaaatcgag agtgacaaac tcgcccgctg
    29461 caacaatctt gtcgctggct ttgccgtgcg gcagcgcccc gcgccagccg ctggcgacaa
    29521 tggtgtcaaa agaggctttt tctgcgccct gctggcgcat aaaccactcc agttcagcgg
    29581 ctatctcgcg ctcgctcatc cccgcctgaa taaagcggcg aatatgctcc gctccgcgat
    29641 cggcaatccc acaggcgagg cggattatct ccacctcctc tggcgttttg atttgccgca
    29701 gcacatccgg cgtggcgcta accagtttcg cattgagttc agacttccag cggtgcgcgg
    29761 tttcccaact cacctgctgg ccttcaaaac cgagcgtttg caactgctca tcggcaatga
    29821 tttgattgac gatagtggta agcgtgttcg ttgcgtcaag caaatgcagc tggtagcctt
    29881 gcgcacgggc ttccacctca acgaaatagc gcgaatctac cagaatgtgc gcactttcac
    29941 ggctaataac cacatagcct gatccggtgg agatccccag atgcggctgt ttgttctgcc
    30001 gtgaggagag aagcaccgca tccagttgtt gcaccttaag ccagttgcgc agcgaagcga
    30061 gtaatgtcat ctggattttc cttcttacag gctatcgatt aacaatttgc ctttgcggaa
    30121 catcatcaga cgcaggaaca ccaccatcag cgcagtaatg attgccccca gcgcgatccc
    30181 cgccatataa acgcccaagt tagtgaccag cggccatgcc cagatagcgg attccgggaa
    30241 ccattgcact gcgcccagcc agacagctgc ggttgagccg acaatcgcgc cgaccatata
    30301 tgacggaata gcggtgatgg ggctttccag cgcaaacgga atcgcaccct cactaatccc
    30361 cataaaggcc aggaacatcg cggtcttacc ctgcggataa agttgagcgt tgaacaggcg
    30421 tttgccggtt aaacgacggt caataatggt cgccagaccc agaccaatcg gcggaataac
    30481 gatagcgata gaacgagcgg tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac
    30541 gaaacctgcg gctttgttga tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc
    30601 cattgcgtac atcagcgcgc ccttctcacc tgcggcagtc agcacagtac ggataccgcc
    30661 gttgatccag ccaccaaagg gagtgatgac gtagtacatc gccagcatga caaaaatggc
    30721 ggaaagaatc ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg gtaactggat
    30781 tttctggttc atccatttca caaggtaacc ggcaacgata gaaataatca gcgcaccaat
    30841 gaaggtggac ggcaccggcg aagaggtcgc ccattgcata gtgctgggat cgaagttcag
    30901 cagttgcgtc ggctgggtag acatcaaccc gccgataaaa ccagccggga acgccagttt
    30961 gccaccgata gagttcgcca caaatgcagc aaacatcgga atggcaaaac caaacaacac
    31021 gccgccgaag gactgcgaca gccaggcaaa tttaagtaag gagaggtcga aaccggagaa
    31081 tttcccgcta ttaagagcat ccatgatgcc gatctcagca ggaattttaa gccagctata
    31141 agcaatcagc tggctgaaag cgaggatcac accgcccata attaaggtcg gcaccatgcg
    31201 tgaaatccct gacatcacat gctgcgggag ttcaccccag aagctgcttt tagaggcctg
    31261 cggattctta actgccgctg ccgcaccgga tgcgccaggc acaacggttg cactgcgttt
    31321 tttaatggcc ataacaatta tccctgtcga atattactgt tgttcagagg caatcatctc
    31381 ttcgatttct ttgatgatgc ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata
    31441 aacgtcgcgt gattcgaaac gttcgttatc ttccggggta actgccacgg aatggatgat
    31501 gatggtcgct tcggcgatat cctgcgccgt caggcgattc tggatgccgt ccgcgccctg
    31561 ggtttcaatt ttcacttcat aaccggcttc taccgccgct tcttccagca cctgagcggc
    31621 cataaaagtg tgagccaggc ccattgggca ggcacataag gcaatcagtt tcttactcat
    31681 cgttaattcc tcgttcagtg atagttgact agtgaaatta tgaacgtgca aaacaaatcg
    31741 ccgttaccag acaaataatg catttactgg agaattaatc ccgctgtacg gaagtgtgac
    31801 gcaggtcggc cttgtgaggg gacacaaaga aaaattgaat taactgtatg atttaaaaga
    31861 ttatcgggag agttacctcc cgatataaca ggaaggattt acagaatgtg acctaaggtc
    31921 tggcgtaaat gtgcaccgga accgagaaga cccggatagt catggacgat gagatacacc
    31981 ggaatatcat ggacatattc tttaaagcgc cctttatctt caaatgcggc acggaaaccg
    32041 gaggctttga agaactcaag gaagcgcggc acgataccgc ccgcaataaa cacgccgcca
    32101 aatgtcccga gattgagcgc cagattgccg ccaaaacggc ccataatgac gcaaaacagc
    32161 gacaatgcgc ggcggcaatc ggtgcagctg tcagccagcg cgcgttcggt aatatctttt
    32221 ggcctgagat tttctggcag gcggttgtca gctttcacaa ttgcgcgata caaattcacc
    32281 agcccagggc cagaaagcac gcgctccgcc gaaacatgac caatttccgc acgcaatatt
    32341 tcgaggataa tggcctcttc ttcactattc ggcgcaaaat caacgtgacc gccttcgccc
    32401 ggcaagctta cccaacgctt atcgacatgg accagatgcg caaccccaag ccccgttccg
    32461 gcaccgtaaa ccgcaatagg cttaccttcg accggttctg cgccaccaaa ctgaatcaga
    32521 tgctcttttt tcagcatcgg gatcgccatc gatacagcgg taaaatcgtt aataatttcc
    32581 agatggctaa aaccgagatt ctttttcatt tcggcaattg agaacgccca ggtatggttg
    32641 gtcattgcca cccagtcacc ggtaattggg caagcgatgg caatacagcc gtctttcacc
    32701 tcgaccttat gttcttcaag ataaacgcga atgaccgctt cgaggctggg gtaatcaagc
    32761 cctgaatagg tcttagcctg cgagatttca ccactggcaa tatcacacag agcaagacgt
    32821 gcgttggtgc cgcccacatc accgactaat gcatactttg tcattcttca actgctccgc
    32881 taaagtcaaa ataattcttt ctcacactgt aaatacctgg gggcataaca acaacgccgg
    32941 aaaggcaggc tccctgtaaa tatcgatctg ggtcacacaa ttactttatc gtttcagcac
    33001 caattgcagc gatgcctttt tgcaagctgg gcaaactaag tatctgaccc cgcataagga
    33061 atagaacatg ctccatccgc gagccagaac catgttgtta ttatcgctcc ccgccgtggc
    33121 aattgggatt gcgtccagtc ttattctgat tgtggtgatg aaaatcgcct cggtattaca
    33181 gaatttgctc tggcaacgac tgccgggaac tctggggata gcccaggatt cacccctctg
    33241 gatcatcggt gtattaacgc taacgggtat tgcggtgggg ttggttatcc gtttcagcca
    33301 gggtcatgcc ggaccagacc ccgcctgtga accgctgatc ggcgcaccgg ttccgccctc
    33361 tgcgctacct agacttatcg tagcattaat tctcggtctt gctggcggcg tcagcctggg
    33421 gccggaacat ccgatcatga ccgtcaatat cgcccttgcc gtcgccattg gcgctcgtct
    33481 gttaccgcgc gtcaaccgaa tggagtggac tattttagcc tctgccggaa ccatcggcgc
    33541 gctgtttggt acacctgttg cggcggcgtt gatattttcg caaaccttaa atggcagtag
    33601 tgaagttccg ctatgggatc gtctttttgc gccgttaatg gcggcagcag ctggtgcact
    33661 tactaccgga ttatttttcc atccccattt ttcactgccc attgcgcatt acggacagat
    33721 ggaaatgacc gatattctca gcggtgcaat tgtcgcggcg attgccatcg cagcagggat
    33781 ggtcgccgta tggtgcttac cacggttgca cgcgatgatg aatcaaatga aaaatccggt
    33841 gctcgtgctg ggtattggcg gatttattct cggaattctg ggggttattg gtggaccagt
    33901 ttcgctgttt aaagggctgg atgagatgca gcagatggtg gcaaatcagg ctttcagcac
    33961 cagcgattac tttttgctgg cggtaattaa acttgccgcc ctggtcgttg ctgccgccag
    34021 tggctttcgc ggtgggcgaa tcttcccggc agtgtttgtc ggcgtggcat tagggttgat
    34081 gctgcatgag cacgttcccg ccgttccagc ggcaataacc gtttcctgcg ctattctcgg
    34141 catcgtgctg gtggtaacac gcgatggctg gttaagtctt tttatggcgg cagtcgttgt
    34201 acccaatacc acattgctac cgctgctctg tatcgtcatg cttccggcat ggctgttatt
    34261 agcaggtaag ccgatgatga tggtcaatcg tccgaagcaa cagccacccc acgataatgt
    34321 ttagcaaaaa atgctctcct ttatgattta agagttatgg cgatgatacg gggctttacc
    34381 tccccgtaat attgcgttaa caggccgctg acggcttcga tcgtgaagga gaataacaat
    34441 gttcaggtca ctgtttctgg cggccgccct gatggcattt accccgcttg cagcaaatgc
    34501 aggtgaaatc accctactgc catcgatcaa attacaaatt ggcgatcgcg atcattacgg
    34561 taattactgg gacggtggtc actggcgaga tcgtgattac tggcatcgca attatgaatg
    34621 gcgcaaaaac cgctggtggc gtcatgataa tggctatcat cgtggctggg ataagcgtaa
    34681 agcatatgag cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc gcggaaaagg
    34741 ccgcggacat gggcaccgcc attaaacgcg tcctccaatg gagcacaatg cctgatgcga
    34801 cgctagcgcg tcttatcagg cctacaaacc ggctctttaa cgcgtagggc ggataaggcg
    34861 tttacgccgc atccgccaat agtgccagat gcgacgctca ttcaactaca atcccagcgc
    34921 cgtccccacc aacaaccaga tattcagcgc cacgaccagc actacaatca cccagcctgt
    34981 ctgttttacg cgtttgctgt tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat
    35041 cagcagtgga accagcgcca gggcgatacc aaaacttaac agcacctgac tcataaccag
    35101 aatccgtgtc ggatctaatc ccatcagaat gacaataaat gacggcaaca tggtgactgt
    35161 acgacgcacc cacagcggga tatggaagcg aatgaatccc tgcatcacca cctgccccgc
    35221 cagtgtcccc accaccgttg aggacagtcc ggcagcaacc agacttaacc caaagaccgt
    35281 tgccgcagca tggcttaaca gcggttgcag cgtcagataa gcctcatcaa gatcggcaac
    35341 accagtatga ccagaaaagt ggaacgccgc cgcagctgta gccatcatca ccagattgac
    35401 aaaaccggca atcgtcatgg cgatagccac atcccatttg gtggcggaat aacgttgttg
    35461 acgcgaaccg ccatgtaaat gctgagtgag cgaagagtgc aaataaatca catgcggcat
    35521 aatcgtcgcc cctaacacgc ctgctgccag gaagaccgcc tccgaagtag gtaaactcgg
    35581 gatcaccatt cctttaccca gctgcgccag gttaggctgg gagaaaatca actcgacaat
    35641 gtaagccgcg gcaacaaaca acagtaaccc gccaatcact ttctccagcg gtttttgccc
    35701 gcgacgttgc agcattaaaa tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa
    35761 caacgaaaca ccaagaatga gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc
    35821 ggttgccatc gcaataattt ctgcctgaac ccaatagaac cacactacgg gacgcggata
    35881 gtgatcacga atctgctccg ccagattttt accggtggca atccctagtt tggcagagag
    35941 gatctgaatc agcatcgcca tcaggttggc ccaaacgaca acccacagta gctgatagcc
    36001 gaagctagca cccgcctgaa tattggtcgc aaagttaccg ggatcgatat aaccaatcgc
    36061 cgcaatgaac gcaggtccca ttaatgcgag cctcatctta cgcgccgccc gtccgctgct
    36121 actctcaacg cgatagttcg tcatcttgtg cctctaaaac atagcctttg ctatgtttca
    36181 tgctatgcta aacgagaatg attatcaaat tcatttaaat ggattgtggt gatttctctg
    36241 atagaccaag attatgatta cgaaaagatt gatgacttga atgttatggt tatgtttaat
    36301 gttagcacat ttacataact ttcagcttcc atacacaaca tagcagaaat gtatgacaga
    36361 tcactatttt tgaagcctgt cacaggacgt cattatagtg tgtgtcagat ctcgttttcc
    36421 ttaaccatgt tacatagaat gtgcacggaa atttaacctg cctcatattt ggagcaaata
    36481 tggaccgcgt ccttcatttt gtactggcac ttgccgttgt tgtgattctc gcactgctgg
    36541 taagcagcga ccgcaaaaaa attcgtatcc gttatgttat tcaactgctt gttatcgaag
    36601 tgttactggc gtggttcttc ctgaactccg acgttggttt aggcttcgtg aaaggcttct
    36661 ccgaaatgtt cgaaaaactg ctcggatttg ccaacgaagg gactaacttc gtctttggta
    36721 gcatgaatga tcaaggcctg gcattcttct tcctgaaagt gctgtgccca atcgtcttta
    36781 tctctgcact gatcggtatt ctccagcaca ttcgcgtgtt gccggtgatt atccgcgcaa
    36841 ttggtttcct gctctccaaa gtcaacggta tgggcaaact ggaatccttt aacgccgtca
    36901 gctccctgat tctgggtcag tcagaaaact ttattgccta taaagatatc ctcggcaaaa
    36961 tctcccgtaa tcgtatgtac accatggctg ccacggcaat gtccaccgtg tcgatgtcca
    37021 tcgttggtgc atacatgacc atgctggaac cgaaatacgt cgttgctgcg ctggtactga
    37081 acatgttcag cacctttatc gtgctgtcgc tgatcaatcc ttaccgtgtt gatgccagtg
    37141 aagaaaacat ccagatgtcc aacctgcacg aaggtcagag cttcttcgaa atgctgggtg
    37201 aatacattct ggcaggtttc aaagttgcca ttatcgttgc cgcgatgctg atcggcttta
    37261 tcgccctgat cgccgcgctg aacgcactgt ttgccaccgt tactggctgg tttggctaca
    37321 gcatctcctt ccagggcatc ctgggctaca tcttctatcc gattgcatgg gtgatgggtg
    37381 ttccttccag tgaagcactg caagtgggca gtatcatggc gaccaaactg gtttccaacg
    37441 agttcgttgc gatgatggat ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag
    37501 gcatcatctc tgtgttccta gtttccttcg ctaacttctc ttcaatcggg attatcgcag
    37561 gtgcagttaa aggcctgaat gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac
    37621 tggtttacgg ctctaccctg gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt
    37681 aaaaccatac ataaaaaagc cggggatcat tccccggctt ttttacgcct gttaatcagc
    37741 taatggctgc gggcgaccaa tcaaataccc ttgcagatat tgcaccccga gcttatgcaa
    37801 tagcgcctgc tgctgttgcg tctcgacaaa ctccgcgacc acactcaatg acttcgcttt
    37861 cgccagatcg gtaattgatc tcacaatcat cgcatccagc gtattcgtga caatatcttt
    37921 cacaaagacg ccatcaattt tgatgatatc agcctgcaaa cgctttaacc gttcgtagtt
    37981 ggcatatccg gtgccaaagt catcaatcgc aatccggaaa ccaaacttat gcagctgctc
    38041 gatgttgtac atgctgcttt ctgcgttaga aaacgcctgc tcctcggtga tctcaagaat
    38101 gaccgcctgc ggggagatgt gataacgctt aaacagacga ataatccgcc cggcaatatt
    38161 cttttgcagc agcgtgagcg gcattaaatt gactgaaaag cgcggtcctt ttttgtcgca
    38221 agggtgtgtt gccagccact tcaacaagga ttccagcact tgcaaatcaa aacgcgcgct
    38281 aaggttaaac tgagcaataa ggggcagaaa cttatccggg gtcataatgc cgccgtcata
    38341 tttcagtcgc gcgaggatct catcataacc ttcaccctct ttgttgcgaa ttggctgggc
    38401 gtagagcagc aaatcaccct gatctaacgc cgtgcgaatg gtattcagca atagcacctg
    38461 tttagtggtc tgcccggaaa ccatctcctc tctgctatcc agcgccagca catgatgatg
    38521 tgcgcaggat tgctccgcca gccagcttaa ctgccccaac aagggttgca gggtttcctg
    38581 atttccatca aaacgccccc aggcagcacc atagcccata tccagcccgg tattgttcca
    38641 gtgaatttgc cgactattca ggatgttaac catatgctgg agtcgccctt ccgtttccgg
    38701 cccgctcagc actaacagca gttcactacc cggcaattga tacaactttt cgttttcctg
    38761 catcaacggc agcagcgtac ggcaaattga gcggatacag tgaacgcgca tcattaagcc
    38821 gtaatgacga ctcataaact caagattatc aatgcgcagg cagcaaaaac tcttgcccgc
    38881 ctcttgctcc ggcgcttgtt ccaacgcacg aaagttgggt aggagcgtta acggatctgt
    38941 tagcgcctgc aaatgccagc ggcgattaag ccattcactg cgatgataaa tgcgcaccat
    39001 atagagcagg caaacgctaa aggaaatcag caccgcgaga ataaatgcca gcgaatattc
    39061 ggtttccacc ccttgcaaaa agttctgatt gtaattcaga aggcaaagcg ttgaaacagc
    39121 ccaggtgaga tttaaaaacg gatagcgaag attaccgacc ccgagggtaa agatgatgaa
    39181 gaaaacgggt accaggtaac cggcaataaa gtcgttttca taaggtgtgc acaacagaag
    39241 tagcagcacg cttaacgctg ccagccagct taaggtaaat gcgagtgaga tagtagaaaa
    39301 gcatgttgta aatcagcact gcggtgaaaa ggcttagtaa atcaacgacc gtgaaaatgg
    39361 catccgcatc gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa
    39421 gatacatgct gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc
    39481 agacatatcg gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg
    39541 cgcaggcaca caacacgaca aacgtctggc tgccgagcat gaccgcagtc tgtaaaaaag
    39601 agagatgaaa gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc
    39661 gccagccaaa gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga
    39721 tataactgga atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca
    39781 cggtgagcgt aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa
    39841 acatgagtac actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc
    39901 gcatcgcgaa gcaaacaggg gccagcctgt tgccgatttt tcatccaatg atgctttcac
    39961 caggcggcgc taacccggac gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa
    40021 aacccccgct ttgcagcgag ggttggaaat ttggtggagc taagcgggat cgaaccgctg
    40081 acctcttgca tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac
    40141 gtaccaaatt tgctgggtgc aaaatttggt ggagctaagc gggatcgaac cgctgacctc
    40201 ttgcatgcca tgcaagcgct ctcccagctg agctatagcc ccgtcacgta aagcttgtcg
    40261 agttgacggg cggcatcata tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg
    40321 tagtttcaat cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag
    40381 tagttattca tgtcacggtt tcctgtaaag tggtgttata aaatgaacta ctaatagacc
    40441 cacatacatt cagggaattg ttatgttcaa ggagaggatg acaccagatg aacttgccag
    40501 actgaccggc tatagccgcc agaccattaa taaatgggta cgcaaggaag gctggacaac
    40561 gtcaccaaaa cctggcgtcc agggtggcaa agcccgactg gttcacgtca atgaacaggt
    40621 tcgtgaatat attcgcaatg ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc
    40681 tggtgatgcg ccgcttgaag tattgctggt gacactggcg aaagagatga cgccagttga
    40741 acaaaaacag tttacatcct tgcttctgcg ggaagggatt atcggattgt tacaacgctt
    40801 agggattcgc gatagcaaat aatatgaaaa gattacgcaa taaaatgacc accgaagaac
    40861 tggctgaatg cctcggtgtg gctaaacaaa ctgttaaccg ttggaccaga gaaaaaggct
    40921 ggaaaacgga aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata
    40981 cgcaagtttg cgagtttatt cagaacacgc cagccttcca taacacacca atgttgatgg
    41041 aagctgaaga gagaattgct gaatacgctc ctggagcacg cgccccggcg tatcgacaaa
    41101 tcatcaacgc gatagataat atgacagaca ttgagcagga aaaagtcgcg caatttttgt
    41161 cacgtgaagg aattcgcaat ttcctcgccc gtctcgatat tgacgaatca gcataaataa
    41221 aaaacggcag gatattatct cctgccgttt atctttttac acgctaatta ctgctgattt
    41281 tcgcgttcag caataaaatc cagcgctttg ttgatacgct cgatactgcg ggtcttacca
    41341 attgcgtgaa cggtaacatc cagtgctgga gactgccccg caccggttac ggcgacacgc
    41401 agcggcatac caactttacc catacccact tccagctcat ccgccgtcgc ctgaatagcg
    41461 tgatgaacgt tttcagcggt ccagtcagta atcgcggcca gtttgtcacg aaccacttcc
    41521 agcggctgac gcgctaccgg acgcagatgt tttttcgccg catcggcatc gaactcagca
    41581 aaatcttcgt agaaataacg gcagctctgt gccatctctt tcagcgtctt gcagcgttcg
    41641 cccagcagtt tcaccagatc agccagctgc gggccgttac gggtatcgat attttcctgc
    41701 tcaatgtgcc actgtaagtg agtagcaaca tactccggcg gcagcgcgtt aatgtagtga
    41761 tggttcagcc acagcagctt gtcggtgttg aacgcactgg cagatttgct gacggcattc
    41821 aaagtgaagt atttgatcat ctcttcacga gtgaagattt cctgatcgcc gtgggaccag
    41881 cccagacgca ccagatagtt cagcagtgct tctggcaaat aaccgtcatc acgatactgc
    41941 attacgctga ctgccccgtg acgtttggac agttttttac cgtcatcgcc attgatcata
    42001 gaaacgtgcg cgtaaaccgg caccggcgct ttcagggcct taagaatgtt gatctggcgc
    42061 ggcgtgttgt tgatatggtc ttcgccacgg ataacgtggg tgatttccat atcccagtca
    42121 tcgacaacca cacagaagtt ataggttggg gaaccatcgg tacggcggat aataagatcg
    42181 tccagttcct ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa aacaacagaa
    42241 ccttcctgcg ggttagcaaa acgtacaaca cacggttcat catcagcatg atgctcatgg
    42301 ctgtggcggc agcgaccgtc ataacgcggc ttctcacctt tcgccatttg ctcttcgcgc
    42361 agcgcttcca ggcgctcttt agagcaatag catttataag cagtgccctc ttccagcatc
    42421 tgatcgatca ccgcgttgta gcgatcaaaa cgtttggtct ggtagtacgg accttcatcc
    42481 cactccaggc tcagccagtt catgccatcc ataatggctt cgatagcttc cggcgtggaa
    42541 cgctcaagat cggtgtcttc aatacgcagc acgaactcac cgccgtggtt acgtgcaaaa
    42601 agccaggagt aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt tgggcttggc
    42661 gcgaagcgag ttttgatttt catgaaatgg ccttacgttt agaaagatgc cgacaaccgg
    42721 caaatcctgg aaaaattgag tgggcgatat tctatcactc ccgcctgatt cctcaatgta
    42781 gttcgggttt ctaccttacg cattattgtt ttcgttgaca aattgcgcaa cacggtctgt
    42841 tttgcgatca tcttgcttaa atttacgacg aacgaacaat ttctttagaa aatgcgttga
    42901 ctcattttga actctcccta taatgcgact ccacacagcg ggggtgatta gctcagctgg
    42961 gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccaact
    43021 actttatgta gtctccgccg tgtagcaaga aattgagaag tgggtgatta gctcagctgg
    43081 gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccactt
    43141 cgggtcgtta gctcagttgg tagagcagtt gacttttaat caattggtcg caggttcgaa
    43201 tcctgcacga cccaccaatg taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat
    43261 actcaaagat tcgaacctgc agcaggtttg agttgagcgc agcgaaacaa cggagccgct
    43321 cgcggcgacg gcccgaaggg cgagcgaagc gagtcatcct gcacgaccca ccaatgcaaa
    43381 aaagcgccct aaaggcgctt ttttgctatc tgcgatttgt aagattgatt tttcttctca
    43441 agttccttgt tcttctaccg atattacctc gtacagtgac gaggactgaa caatggccac
    43501 tatctcttca acttcaattc cttccataca aacacagagt agtaacaggg catcacaagg
    43561 tagcgatgtt gcttcacaaa tcgcgcggat aagtcagcaa atcatcaaac tcactcaaca
    43621 aattaaagag attgtagaca ctagcggcag tgctgaagac aagcaaaaac aggccgaatt
    43681 gattcaacag caaatcaccc tactggaaac acaactggcg cagttgcaga aacagcaggc
    43741 agaaaaggcg caggagaaag agcagcgttt gtctctgaat gtatcccttc taaatcctgt
    43801 tgaaaaaacg actcatatcg atatctatat ctgatacaac ggatcccctt cccgaccagc
    43861 cattagcgca ttgccctggt ctcgtaccac gtcccataac gcttcagcgg cggtggaaag
    43921 tgaacggttc ttcctgcgca ccagcatcaa ctggcgttca accaccggcg taatgcgctt
    43981 aaccaccagt gggctacctt caggcaatgg tagcgccagt gcaggaagaa tactgatgcc
    44041 gatgcctgcg gctaccatcg ggaacagcgt cgccggatgt ccaatctcct gcacaatatt
    44101 cgcctgaata ccattgcgcg ccagcgctgc gtcaatcagc ggtcggctgc ctgacgcgta
    44161 atcctgcaac accaatttcg ctccctgtaa tgcctgccag ggtacataat cttccacggc
    44221 caaagcgcta tcgcgatggc aaagcagaaa gaaaggctcg gaaagaatcg cttcacattg
    44281 cagatcgccc actgggcctg gatcgatgac gatgccaaag tcgacatctc cctggcgaat
    44341 actttccatc acccactgct gcggacgatc atgcaggaca aactgaatat ctggatagcg
    44401 gcgatggctt tcagcaatgc attgcggaat aagatgcgcg gaaatggtct ggctggcagc
    44461 gacccgaact tttccgctca gttgttgccc catacgcccg gtatcgcgca acgtgctgtt
    44521 aagttcatcc agcagtcgct caagacgcaa agccaactgc tgccctgcat ctgtaagcac
    44581 cacttcacgc gtggttctgt ccagcaggcg aacaccagta tgattttcca gctccttcac
    44641 actgtgactt actgccgact ggctcaagcc gatacgctct cctgcacgac taaaactttt
    44701 ctcctgcgct actgtgacga aaacctttaa ttgttttaaa gaataattca tctgttttct
    44761 tcatgaatgg atgcaataaa tcaattttat ttctcaaact ggaagaagca caatagaacc
    44821 atcgatcatc tggagtcttt atgaaacttt ttcgtatcct cgatcctttc accttaaccc
    44881 tgatcacggt ggtgttgctg gcctctttct ttccggccag aggcgatttc gtccccttct
    44941 ttgaaaatct gaccaccgca gctattgccc tgctgttctt tatgcacggc gcgaagttgt
    45001 cgcgtgaggc gattattgct ggcggtggtc actggcgact gcatttgtgg gtaatgtgca
    45061 gcaccttcgt gctgtttccg attctgggtg tactgtttgc ctggtggaaa ccggtaaatg
    45121 tcgacccgat gctctactcc ggttttctct acttgtgcat tctcccggct accgtgcagt
    45181 ctgcaatcgc cttcacgtca atggcgggcg gtaacgtcgc ggcggcggtt tgttctgcgt
    45241 cagcatccag cctgctgggg attttccttt caccattgct ggttggtctg gtgatgaatg
    45301 ttcacggtgc aaggggcagc cttgagcagg tcggtaaaat tatgctgcaa ctgctgctgc
    45361 cgtttgtgtt ggggcatctt tcccggccgt ggattggtga ctgggtgtcg cgcaataaaa
    45421 aatggattgc gaaaactgac cagacgtcca ttctgttggt ggtttataca gcgttcagcg
    45481 aagccgtcgt taatggtatc tggcataaag ttggctgggg atcattgctg tttatcgtgg
    45541 tggtcagctg cgttcttctg gctatcgtga ttgtagttaa cgtctttatg gcacgccgac
    45601 tgagcttcaa taaggcagat gaaattacta tcgtcttttg tggttcgaaa aagagtctgg
    45661 caaatggcat cccgatggca aacattctgt tccccacatc ggtgatcggt atgatggtgc
    45721 tgcccctgat gattttccat cagatccaat tgatggtctg tgcggtgctg gcgcgtcgat
    45781 acaaacgcca gaccgaacag ttacaggcgc agcaggaaag cagcgccgat aaagcttaaa
    45841 gcggacgctt caggggctgg accagttgcg tcagcccctc ggttttaatc agcagcgtga
    45901 tttgcattag ctcaccgagc ttccctgccg ggaactcatc tttgcgggca aaccacagca
    45961 gatactcctc cggcaggtca attaagcgac gccctttgta tttgccaaac ggcattatcg
    46021 tattggctat ttcaatcagc tgctcttttt ccatctcagc tacccagcaa acgcagcatt
    46081 tccgcttcgt cgatgacttc aatgcccagt tcctgcgcct tcgccagttt agatcctgca
    46141 gcttcacccg ctatcaccag atcggttttc ttcgacacgc tgcccgcgac tttcgcgccc
    46201 agttcgacca gtcgagcttt agcgtcatca cgcgacatct ggcttaagct gcccgtaagc
    46261 accacggttt taccagcaaa cgggctgtca atctcttccg cgttgataac gatcggcgca
    46321 ggccagtgaa caccttccgc caacagctcg ctgatgacat tgcggttgct ttcttcggca
    46381 aagaagttgt gaacgtggga tgcaacgaca atgccaacat caggcacctt ttgcagctct
    46441 tcaatcgaag cggcttccag cgcttccagc gtgccgaaat atgccgccag acctgctgcg
    46501 gtggcctcgc cgacttcacg gatgccaagt gcatagagga agcgagcaaa ggtggtttct
    46561 ttcgcttttt ccagcgcgtt aaccacgttt tgtgccaatt ttggccccat acgctccagt
    46621 ccggtcagtt tgcctgcggt gagtttgaac agatctgccg gagtatggac atattctttt
    46681 tcaaccagct gatcgatgat tttgtcgccc attccgtcaa catccatcgc acggcgggaa
    46741 acaaagtgtt tcagcgactc tttacgctgc gcaccgcaaa tcaggccacc ggtacagcgg
    46801 gcaaccgctt caccttccac acgctcaacg tcagaaccac ataccggaca atgcgtcggg
    46861 aatacaacct cacgggtatc ttccgggcgt tcagaaagca cgacgttaac cacctgcggg
    46921 atcacgtcgc cagcgcggcg aatcaccact ttatcgccaa tgcgtaaacc aagacgttcg
    46981 atttcatccg cattgtgtaa ggttgcgtta ctcaccagca cgccagcaac atggacaggt
    47041 tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa cctgaaactc gacgtcacgc
    47101 acaaaggtca tctgctcctg cgccgggaat ttaaacgcaa ccgcccagcg cggggcacgc
    47161 gcgacaaagc caagctgctc ctgctgttcc agtgagttga ccttaatcac cacgccgtcg
    47221 atatcaaagc ccagcgtcgg gcggtcttct tccactttgt gatagaacgc cagcacttct
    47281 tccgccgatt cacaaagcgt tacccgatcg ctgaccggca ccccccactt tttaaattgc
    47341 agtaaacggc caagatgagt atccggcagc tcgccacctt ccagaacacc aacgccatag
    47401 cagaaaaaag tgagcggtcg cttcgctgta atacgcggat caagctgacg cagtgaacca
    47461 gctgccgcat tacgtgggtt agcaaaaact ttcccgcccg tgcgtcgcgc atcttcgtta
    47521 atcttttcga accccgcctg cggcaggaac acttcaccac gcacttccag acgcgccggg
    47581 atattctctc cgtgcagctt cagcggaatg gcgcgaatag tacgcacatt agacgtgata
    47641 tcttccccgg tggtgccatc gccacgggtc gcggcactga ctaaaacgcc attttcatac
    47701 agaatactga cggcaaggcc atccagcttc agctcacagc accaggtgac tttctcgttg
    47761 tttttcagac ggtcctgcac acgtttgttg aaagcaagaa agctttcttc atcaaaaacg
    47821 ttatccagtg acagcattgg tacttcatgg cgtatctggc tgaaagctgc cagcggcgca
    47881 gcacctacac gttgggtagg cgaatcaggc gtaatcagtt ctggatgttt ggtttccagc
    47941 tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg gaatttccgg cgcatccatc
    48001 acatgataaa gatattcatg atggcaaagc gtcgttcgca gttctgtcag ttgttgttcg
    48061 attgattcca tatcgcacca tcaatgctaa aaacccccga caagcggggg ttcgaagagg
    48121 agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg acttcgcgga tgatgtcctg
    48181 gtactcgcgc aatttctgcg gagtcatcat acggcgctga tcgtcaagca cgacaccgcc
    48241 cacttcatcg gcaatatgct gcgcagattg cagcatcagc ttgaagttct gcagctcgtc
    48301 accgtaagac ggtacctgca taaagatagt gacacccgga gtagtgaaat ccttcatttc
    48361 aggatcaaag gttcccggtt tcaccatatt cgccaggctg aataacgccg ggccgctgcc
    48421 atccgggcta agatgacgat ggtaaatatt catatcgcca aaaatgaagc ccgcttgttg
    48481 aatgctgtta agaagcagtt caccgtttag ctcgctaccg tgatgcgccg cgacgttcat
    48541 gataatcacc gcttctttgc gcttcggttt atccataact ggagccggtt ccgctacagg
    48601 ctcaggctgt ggtgccgcta cgggttctgc aggctggaaa gcctgttgtg ccggttgcgg
    48661 tgctgaatgc acaggctgcg gcgctggcgc gacctgtggc gaaactggct gctgcaacgg
    48721 ctgttcaggc tgcggctgat aggcaggctg ctgcaccggc tgcgctggac gcggagcatg
    48781 ttgcggcggt acctgcgctt caggcggctg ctggaccggt tgacgcggct gcgcagacgc
    48841 ataaggcggt aggtactggt gttgcggcga cggacgagca gcctcatgct cctgagcgtt
    48901 agccggggca tgattcacgc ggtgaacacg aacctcacca acgccctcat catcttcgac
    48961 atcctcgtca taagaatcgt cgtcacgttt tgacttcatt cgttttaatg gccgatcgcg
    49021 gaacatagaa gatcgttctt tacggctggt ccagaaacca tgtaccagta aagcgattat
    49081 ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc tgcatcatta tattctctgt
    49141 tgttctaaca ccttgccacc acggcaaaca tttactcact aagagtattt gccgattacc
    49201 tcaagtgcaa gtgcactatt aactttcaca gcacaaagat agatgaaatc gtgctttttg
    49261 ctgttttttc gaacatatcc taactgtcca ttgcgcaatt acccggtaaa atacgcagaa
    49321 ttttcctggg attggtcaaa aggagctcat cctgattatg gtttcatcat tcacatctgc
    49381 cccacgcagc ggtttttact attttgcgca aggctggaag ctcgtctcgc aacctgggat
    49441 tcggcgtttc gttattttac cgctgctggt caatattttg ttgatggggg gcgcattctg
    49501 gtggctcttt acacagctcg atgtctggat cccgactttc atgagttacg ttccggactg
    49561 gctacaatgg ctgagttatc tgttgtggcc tctggctgtc atctctgtgc tgttagtgtt
    49621 tggctatttc ttctccacga ttgctaactg gattgccgct ccgtttaacg gtttattggc
    49681 tgaacaactg gaagcacgat tgactggcgc tacaccgcca gataccggga ttttcggtat
    49741 catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgtct ggtatctgcc
    49801 gcgcgcaatt gtattactaa ttctttactt catccctggt attgggcaaa ccgtcgcgcc
    49861 ggtactgtgg ttcctgttta gcgcctggat gttagccatc cagtattgcg attacccctt
    49921 cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac gcaaaatcac
    49981 caatatgcag tttggtgctt taaccagcct gtttacgatg atcccgctgc ttaatctgtt
    50041 catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct atcgcgataa
    50101 acacgcgatg tggcggtaac aatctaccgg ttattttgta aaccgtttgt gtgaaacagg
    50161 ggtggcttat gccgcccctt attccatctt gcatgtcatt atttcccttc tgtatataga
    50221 tatgctaaat ccttacttcc gcatattctc tgagcgggta tgctacctgt tgtatcccaa
    50281 tttcatacag ttaaggacag gccatgagta agatttttga agataactcg ctgactatcg
    50341 gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg acgcattctg gcgaaggtgg
    50401 aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg tgccaacatg atttgggatg
    50461 ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt tgaaccgacc agcggtaata
    50521 ccgggattgc actggcctat gtagctgccg ctcgcggtta caaactcacc ctgaccatgc
    50581 cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca aacctggtgc
    50641 tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa attgtcgcca
    50701 gcaatccaga gaaatacctg ctgctgcaac aattcagcaa tccggcaaac cctgaaattc
    50761 acgaaaaaac caccggtccg gagatctggg aagataccga cggtcaggtt gatgtattta
    50821 ttgctggcgt tgggactggc ggtacgctga ctggcgtcag ccgctacatt aaaggcacca
    50881 aaggcaagac cgatcttatc tctgtcgccg ttgagccaac cgattctcca gttatcgccc
    50941 aggtgctggc aggtgaagag attaaacctg gcccgcataa aattcagggt attggcgctg
    51001 gttttatccc ggctaacctc gatctcaagc tggtcgataa agtcattggc atcaccaatg
    51061 aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga aggtattctt gcaggtatct
    51121 cttctggagc agctgttgcc gcagcgttga aactacaaga agatgaaagc tttaccaaca
    51181 agaatattgt ggttattcta ccatcatcgg gtgagcgtta tttaagcacc gcattgtttg
    51241 ccgatctctt cactgagaaa gaattgcaac agtaatgcca gcttgttaaa aatgcgtaaa
    51301 aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc tcctggcatt
    51361 gattcagcct gtcggaactg gtatttaacc agactaatta ttttgatgcg cgaaattaat
    51421 cgttacagga aaagccaaag ctgaatcgat tttatgattt ggttcaattc ttcctttagc
    51481 ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac acccgccagc aatggactgt
    51541 attgcgctct tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca ggctaaagtc
    51601 gaaccgccag gctagacttt agttccacaa cactaaacct ataagttggg gaaatacaat
    51661 gttccagcaa gaagttacca ttaccgctcc gaacggtctg cacacccgcc ctgctgccca
    51721 gtttgtaaaa gaagctaagg gcttcacttc tgaaattact gtgacttcca acggcaaaag
    51781 cgccagcgcg aaaagcctgt ttaaactgca gactctgggc ctgactcaag gtaccgttgt
    51841 gactatctcc gcagaaggcg aagacgagca gaaagcggtt gaacatctgg ttaaactgat
    51901 ggcggaactc gagtaatttc ccgggttctt ttaaaaatca gtcacaagta aggtagggtt
    51961 atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct gcttctgaaa
    52021 gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg accaggttga tcaggaagtt
    52081 gaacgttttc tgagcggtcg tgccaaggca tcagcccagc tggaaacgat caaaacgaaa
    52141 gctggtgaaa cgttcggtga agaaaaagaa gccatctttg aagggcatat tatgctgctc
    52201 gaagatgagg agctggagca ggaaatcata gccctgatta aagataaaca catgacagct
    52261 gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga gctggatgat
    52321 gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct gctgcgcaac
    52381 atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat tctggttgcc
    52441 gctgacctga cgccgtccga aaccgcacag ctgaacctga agaaggtgct gggtttcatc
    52501 accgacgcgg gtggccgtac ttcccacacc tctatcatgg cgcgttctct ggaactgcct
    52561 gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa atgacgacta tctgattctg
    52621 gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg aagttattga taaaatgcgc
    52681 gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa agatctgcca
    52741 gctattacgc tggacggtca ccaggtagaa gtatgcgcta acattggtac ggttcgtgac
    52801 gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac tgagttcctg
    52861 ttcatggacc gcgacgcgct gcccactgaa gaagaacagt ttgctgctta caaagcagtg
    52921 gctgaagcgt gtggctcgca ggcggttatc gttcgtacca tggacatcgg cggcgacaaa
    52981 gagctgccat acatgaactt cccgaaagaa gagaacccgt tcctcggctg gcgcgctatc
    53041 cgtatcgcga tggatcgtaa agagatcctg cgcgatcagc tccgcgctat cctgcgtgcc
    53101 tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga agaagtgcgt
    53161 gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg taaagcgttt
    53221 gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac aattgcacgt
    53281 catttagcca aagaagttga tttctttagt atcggcacca atgatttaac gcagtacact
    53341 ctggcagttg accgtggtaa tgatatgatt tcacaccttt accagccaat gtcaccgtcc
    53401 gtgctgaact tgatcaagca agttattgat gcttctcatg ctgaaggcaa atggactggc
    53461 atgtgtggtg agcttgctgg cgatgaacgt gctacacttc tgttgctggg gatgggtctg
    53521 gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat ccgtaacacg
    53581 aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac aacggacgag
    53641 ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc cacgagatgc
    53701 ggcccaattt actgcttagg agaagatcat gggtttgttc gataaactga aatctctggt
    53761 ttccgacgac aaaaaggata ccggaactat tgagatcatt gctccgctct ctggcgagat
    53821 cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg gtgatggtat
    53881 tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta gacggtacca ttggtaaaat
    53941 ctttgaaacc aatcacgcgt tctctatcga atctgatagc ggcgttgaac tgttcgtcca
    54001 cttcggtatc gacaccgttg aactgaaagg cgaaggcttc aagcgtattg ctgaagaagg
    54061 tcagcgcgtg aaagttggcg atactgtcat tgaatttgat ctgccgctgc tggaagagaa
    54121 agccaagtct accctgactc cggttgttat ctccaacatg gacgaaatca aagaactgat
    54181 caaactgtcc ggtagcgtaa ccgtgggtga aaccccggtt atccgcatca agaagtaatt
    54241 cttgccgcag tgaaaaatgg cgcccatcgg cgccattttt tatgcttccg ccagcggcgg
    54301 caaaatcaat tcatcgctct catgctgctg ggtgtagcgc atcacttcca gtacgcgcaa
    54361 ccccgcacgg tgcaccgcat cgtttaacgc cttccctttc agcaaaccac tgataagctg
    54421 agcacaaaac aggtcgccag tccctttcag gtcggttttt acccgtgaat gggaaatgac
    54481 attcacgctg tcggcactga ccaccaccac ctgcatctcc tgattttctt cattaccgga
    54541 ggcgctggta atcaccaccc attttaatgt gtctgaaagc agactttttg cggcagcaat
    54601 ggcactgtcg agatcgcggc aatttttacc ggtcaggatt tccagctcaa agatattggg
    54661 ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac gcctcgggaa ggtcaggttt
    54721 gacataaatt ccgctatcaa tatcgccaat caccggatcg accatgatca ataggtcagg
    54781 atggtctttg cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg atgccgttcc
    54841 catatagccc gtggttacag cacgaagttg gcgcagcgca tcacgctcct gaagcgcacg
    54901 caaatagccg ctaaaccatt cgtccggaat cgcaccaccg tagaaagtgt cataatgcgg
    54961 cgtattgctc agcaataccg tcggcacggc aaagacattc aagccgttct gttttatagc
    55021 aggcaccgca atgctgttgc ctacgcttcc gtacactacc tgcgactgca cggcgacgat
    55081 atccgcctgc agcgccctac tcttatcgtt aaacaacaac aaactactca tttaattttt
    55141 tctccttgcc gatgatcctc atcgtattcc aaccgaaact ttacctgatt ctggcagtca
    55201 aatcggctat tacaaaacaa agataaggta attcagtgaa gaaaatcatt tgtctggcca
    55261 ttacgctgct aatgacactc cccgcttacg cgaagttaac tgttcatgaa gaagcccgca
    55321 tcaacgccat gctggaggga ttagcacaga aaaaggattt gatatttgtg cgcaacggtg
    55381 atgaacatac ctgcgatgaa gcggtttccc acctgcgtct gaagctcggc aatacccgta
    55441 accgcattga cactgccgag cagtttattg ataaggtagc ttcgtcgtca tcgattaccg
    55501 ggaagccgta tatcgtgaag atccccggta agagcgatga gaacgcacag ccttttttac
    55561 atgcgttaat tgcgcagacg gataaaacgg tgcctgcgga aggaaattaa tccgctttgg
    55621 taaggcattt acaggaggta acatgaaaaa atgctttatt tatcacgatg aaaaatcgaa
    55681 taaattttgg tggatagatt acgaagggga tagtttagct gtcaactatg gcaaggtagg
    55741 tagtattggt aaattccata caaaagaatt cgataatgaa gaacagtgtc tgaaagaagc
    55801 cagtaaattg attgccgcaa aaatgaaaaa aggatatcaa gaaactccag actttaactt
    55861 catggatctc tactattttg atgatgaaga gattgggtta catcttaaaa cgtcacaccc
    55921 aaacttccag tgccatttta ctgatccact ttatatgtgt tgctgggatg aagaatctcc
    55981 ttttggtagc gatgaaggtg ctgatgctct aaacgaactt gaaaatagcc tccgttctgg
    56041 actgtgctga tttccctcaa atgttaattg aaactatgtg gggtatgaaa tacatcgcta
    56101 tggacagtat tcttgaagag gatgtgcgtg cgcaattact agccgatgaa atgagctcta
    56161 tccagagcaa tatgattacc tacgcgactg cattcggtca gatcaaagtc atgggtaaaa
    56221 tctcccataa acttaaaaag atgggactca atgcactagc gcgtcatcag cttaccgcaa
    56281 aaattcttca atggggtgac ggtcaggact caccaatact tcaaaaaatg attgatgacc
    56341 ttactgcttt tccacacgaa aattaaaaac tgcatttgtc ggcagcaaca actgccgaca
    56401 aatcagatta ttgacaaagt gcactttgtt tatgccggat gcggcgtaaa cgcctgatcc
    56461 ggcataaaaa tattgctaat tcaatatatt gcagaaacat tgtaggcctg ataagcggag
    56521 cgcatcaggc agttttgcgt ttgtcatcag tctccaatgc tattaatcct taaatccctg
    56581 ccccctgact aaaatgctct tccccaaaca ctccggtgga gaggtagcga tcgccacgat
    56641 cgcagatgat cgctaccacc accgcaccag ggttagcttt tgccacccgc agtgctccag
    56701 caaccgcgcc gcctgagctg acgccacaga atattccttc ccgcaccgcc agttcgcgca
    56761 tggtgttttc cgcatcgcgc tgatgaatat ccagcacctc atccaccagc gcagcgttga
    56821 aaatccccgg cagatattcc gcaggccagc ggcgaatgcc cggaatactg ctgccttctt
    56881 ccggttgcag gccaacaatg gtcaccggtt tggattgttc gcgcataaag catgaaacgc
    56941 cggtgatagt gccggtcgtc cccatgctgg agacaaaatg agtgatgcgc ccgccggttt
    57001 gctgccagat ttccggcccg gtggtggtgt aatgcgcata agggttatcg ggattattga
    57061 actgatcgag cagctttcct tcgccacgat tcgccatctc cagcgccaga tcgcgcgcac
    57121 cttccatgcc ctgctctttg gtgacaagaa tcagttccgc accataagca cgcatcgccg
    57181 cacggcgttc ctggctcatg ttgtcgggca tcagcaattt catgcgatag cctttcagcg
    57241 cggcaatcat tgccagcgca atgccggtgt taccactggt ggcttcgatt aagacatcac
    57301 ccggtttaat ttccccgcgc ttttccgcct cgacgatcat cgaaagtgcc gcacgatctt
    57361 tcaccgaacc tgccgggtta ttgccttcca gttttaacca cacttcactg ccgttatccg
    57421 gccccattcg ctgcaacttc accagaggcg tattgcctat tgtttgttct aatgtactca
    57481 caatctctat ccatacgtgg tgttgcctga tgcgacgctt gcgcgtctta tcaggcctac
    57541 aggttacaaa ccttgccatt aaaaagcccg gatcgcggga agcgcctccg ggcgtttaac
    57601 attcactcaa cctatcaggc gctttgtgcg agagcaagtt cctcatcgcg ggtttcaata
    57661 cgctcgtcgc cgttatacag ccgcgcatgt tgcagaccaa cgaataaacg ctcgccatgc
    57721 tgcggggcat cgtcgccatg catcacgacc gtcagcggtt cgttgtacca ccccagcggc
    57781 tgcaccacta attgggtgta gtgacctttc gggctggctt ccagtacctg taccggcagc
    57841 ggcgaatcga gactggtacg gcggctgata tccacttccc acgggcgcag gaagagatcc
    57901 accggcccct gatacgcagg tgtgtagccc agcggccagc gatgcgcgcc aacatggaac
    57961 tgcccgccgc gaatggttcc ctgcaggcgg ttcacttcgc ccataaattc gagtacaaaa
    58021 cgggtcgctg gttcgcgcca tacctgattc ggcgcatcag cctgttcaat attcccctga
    58081 ctcatcacca ctacacgatc ggctacttcg gtcgcctctt cctggtcgtg ggtcacaaac
    58141 acgctggtga atttcagctc ttcatgcagt tgacgcagcc agcgacgcag ctctttacgc
    58201 acctgcgcat ccagcgcgcc gaacggttca tcaagcagca gaatttgcgg ttctacagcc
    58261 agcgcgcgcg ccagcgccac acgctgtttc tggccgccgg aaagctgcgc cggataacga
    58321 tccgccagat gcgcaagctg gaccatttcc agcaattttg tcactttcgc tttgatggct
    58381 gcggcattcg ggcgctcgcg acgcggcagc accgtcaggc caaaagtgat attgtcgaat
    58441 accgtcatat ggcggaacag cgcgtaatgc tggaacacga aaccgacttt acgatcgcgc
    58501 gcgtgcaggc gactcacgtc ggtgccgtgg aagcgaatat gcccgctggt ttgatgctcc
    58561 agcccggcga taatccgcag cagcgtggtt ttcccggaac cggacggccc cagcaacgcg
    58621 accatctgac cagaaggaat atccagtgag atatcgttca gcacctgggt gcgaccaaaa
    58681 gacttcttga tattggcaat ctcaatgctc atgatgctcc tcctgctgcg cgcgtttttc
    58741 ctgattctcc aggcgccact gcaacatact ctttaaaaac agggtgataa tcgccatcaa
    58801 cgtcaacagc gcggcggcgg taaaggagcc gacggtgttg tagtcctgct ccaacaattc
    58861 aatctgtaac ggcagcgaaa gtgtttcgcc gcgaatcgag ccggaaacca ccgacactgc
    58921 accaaactcg ccaatcgcgc gggcgttggt cagcaccacg ccgtaaagca gcgcccagcg
    58981 aatattcggc aatgtgacgc gacggaacat ctgccagccg gacgcgccaa gcaaaatcgc
    59041 cgcttcgtct tcctggctgc cctggcttaa catcaccggc accagctcgc gcaccacaaa
    59101 cggacacgtc acaaagatgg tgaccagcac cattccgggc caggagaaca taatttgcag
    59161 attatgttca tccagccagc cgccgagcgg gccgttggag ccgtagaaca gcagatacac
    59221 cagaccggca accaccggcg atacagcaaa cggaatgtcc agtagcgtca gcagtaactg
    59281 gcgtcccggg aagttaaagc gcgtcaccag ccaggccagc agaatgccga acaccaggtt
    59341 taccggtacg gcaatcagcg cgatcatcac cgtcagccag atggcgtgca gcatgtccgg
    59401 atcggccaga ttctgtaaaa ccggcatcag ccccttgctg aatgcctgca cgaagatgta
    59461 aatcatcggc accagcagga tgaacgccga aaccagcatc ccgatgccaa tcagaaacca
    59521 tttgccccag ttaatcgggc gcgcgtcata acgcttcaat tgggtaactt ccgccattaa
    59581 tgacctacca cacgccgacc aaagcgactt tgcagagtgt taattgagaa cagcagcagc
    59641 agagatgccg cgaggatcac cgaagcaatc gcgctcgctg ccgggtaatc aaactcctgt
    59701 aagcgcacaa aaatcatcag cgacgtcact tccgtcttcc acgcgatatt tccggcgata
    59761 aaaatcaccg cgccaaattc accaagacta cgggtaaacg acagcgccac gcccgccacc
    59821 agcgccggag aaagctccgg cagcaccact ttgcagaaac tctgccagcg cgttgcacca
    59881 agcgtttccg ccgcttcttc atattccggg cctaactctt ccagcaccgg ctgcacggta
    59941 cgcaccacaa acggaatgct ggtaaaggcc atcgccaccg caataccaag ccaggtatag
    60001 gtgactttga tatcaaactt cgccagccat tcaccgtaaa aaccgtttac ggaaaagagc
    60061 gaggccagcg ttaaaccggc gactgccgtt ggcagcgcaa agggtaaatc catcagcgca
    60121 tcaagcagcg tgcggcccgg aaagcgataa cgtgttaaaa tccacgccat cagcagaccg
    60181 aaaacgccgt taaaaatcga tgccacaaac gccgacagca gcgtcacttt gtaggccgcg
    60241 accacctgcg ggttggtgat cacctcccag tactgcgccc agctcatctc agaaagctgc
    60301 atcaccagcg cagagagcgg cagcagcaat atcaggcaca caaacagcag actggtgcca
    60361 aggcttaaag taaagcccgg cagcacgcgt cttgaagaaa ctgcaaacat tacttacgcc
    60421 ccgccgctaa cagcttgtct aactcgccgc cgctggtgaa gtgggttttc atcacttccg
    60481 gccatgagcc aaatttgtct tccacgcgga acagttcggt ctgcgggaat ttatctttca
    60541 gtttgtccat gacttccggg ttattcacac ggtagtaata gtcggtgatg atggtctgcg
    60601 cctgtgggct gtagagccag ttcaggtagg cttttgcggc tttttccgta ccgttggcct
    60661 gcacattttt atcgacccac gccaccggga attccgccag aatattggtt ttcgggatca
    60721 ccacttcaaa gccctgtgct tcatactgtt tacggatgtt gttcacttcc gattcaaagc
    60781 taatcagcac atcgcccaag ccacgctcgg caaaagtggt ggttgcgcca cgaccgccgg
    60841 tatcgaacac ttcaacgttt ttcaggaact gggtcataaa ctgttcggtt ttggctttat
    60901 cgccaccgtc agctttatcc gctgcgcccc atgccgccag ataggtataa cgcgcgttac
    60961 ccgacgtttt cgggttcggg aaaatcagct tcacgtcgga gcgcaccagg tcgttccagt
    61021 cgtggatatt cttcgggttg cccttacgca ccaggaagcc catggtggag tagaacggcg
    61081 agctattatt cggcaggcgc gactgccagt cagccgggat cagcttgcct ttgtcgtgca
    61141 ggatttgtac gtcggtcacc tggttataag tgacaacgtc tgcttttaag ccctgcaaaa
    61201 tcgccagcgc ctgttttgat gacccggcat gagattgttt tatcgtcagt ttgtcgccgc
    61261 cgttatcttt tgcccattgc tgttcaaacg gcggattcag ggcggcaaac agctcgcggg
    61321 agacgtcata agaactgttc agcagttccg ttgcctgtac atggcccgcc agcagcagag
    61381 aagcgaccag cgcgagtgag ttctttttca gtaagttaac ggccattgcg cacccttata
    61441 aatttaatga ctttctaata gccatcatat ttataacgga cgtgaaagga gtaacggttt
    61501 tatataccgt ttggtgattt ggaagttaaa aagggaataa gaacttggtt caacaggcgt
    61561 aattgttgca ggcagtttgg acacggacag cgcggagaaa ccggagcgta cacatagtac
    61621 gtgaggattt cgagcactgc ccggggccaa aatgacaaat gaaatagcct ggtgaactta
    61681 gttcaagacg aaatcctccc cacacatgca gggaggaaac agaggtgaat cagataccga
    61741 cgctaaccgt ctccggcagt gtgctgccgc catcaatcac attctgtgta ccggttaaat
    61801 agctggattc atccgatgcg aggaaggccg ccagttcgcc gacttccagc ggatcggcga
    61861 ggcgacacag cgggattgct tttgccattt cagtcagcac cgattctgga tcttccgggt
    61921 tcgactggcg ggcaatgctt tccgccattg gcgtgcggac gtatcccggg cagatggcgt
    61981 taacgcgaat accggattgc gcgtactcca ccgccagcga tttagtcagg ccaacaatcg
    62041 ccgctttcgt taaggcatac gccgtttcgc caggatcggc caccatatca ccagtgactg
    62101 aagacatcat cacaatgcga ccatctttgc gcgcaatcat ctccggcagc accgccttcg
    62161 tgacgttcca tacgccttta atattgatat caatatggaa atcgcgatct tcatcgctca
    62221 tatcgaggaa actgcccaga cgacaaacgc ctgcgttatt caccaggata tcaatgcgcc
    62281 cttctttttc cttcgcgcgt ttgatagctg cggctaccga cgccgggtca cgcacatcgg
    62341 cgacaaccgc cgtacagcga tgaccacgac cacacagttc gtccgctagc ttttcgatct
    62401 caggggagat atccagcaag attaggttcg cgccatgacg tgcaaaagtt ctggcaattc
    62461 cttcgccaat tccctgcaat gcgcccgtaa tcagtgctgt cttgcccgtg agtttaccca
    62521 ttttaatgct ctccttgtta atacagagcg ttacactcca ttccctctga aaatacagga
    62581 taaacaaaat attgctgcgg atcagctcac taattcagga aaatctcaat ccagtcgctg
    62641 ctttatgcat catttcaggc gttgtgttag ctcactgctg cgctgaatca ttttcaatga
    62701 cgccacatca ttgatgttgc acaagaccaa caaacagtaa atcggtaaca gaattttgcg
    62761 ccgtgcgggt agacatcgac gagctacgcc attcggtttc accggaaacg gtatcgagcg
    62821 tgaaatgcgc cagccgccgc agcggagagt cagccagaga agtaatggca atcaccgttg
    62881 ccccctgctt tcgtgccgct tctgcgcaca agacaatctc ttttttactg ccgctgtaag
    62941 agatagcgat ttgtacatcg ccttttttca acgcctggga aacggtggct tgtacgtgcg
    63001 tgtcggcttc gcaggccacg cgatagccaa ttttcatcag tttgaacgac agatcgcgcc
    63061 ccaccagcgc cgatccgccc aggcccgtta tctggataaa cggtgcttta ctgatgacct
    63121 caatgatttt ttgcagccgc gcgtagtcga acaacgcaca ggtttgctcc agcgccagct
    63181 ctttttcgcg attcagtttg cgggcaatca cttccagcga atcatcactg gtaatcgaac
    63241 tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct ggcactgtat tcgccaatta
    63301 acgccatacg caattcagta aatccttgcg cgccgagctt ctgggcaaat ttcacaatgc
    63361 tcgactggct gataccgagc tgtttcgcca tctgccgtga agagacggat ttcagctcgc
    63421 tcactctggc ccgcagaaag tcagcaattt tttgctcgtt cccggtaaat tcactttccg
    63481 cattgcgtat tttcgtcagg tacaacatct tccactctcc tgggctggca gcaaaaattc
    63541 cgcttaagtt cttatgacgc tcttcacact ctgcgagtgt agactcaatg attcctttaa
    63601 gtctgtcaat tcgggatata tgattccata catctgcaat tatttgatcg taaatagtaa
    63661 ggtcaccacc gatgcaacta gaaaagatga ttactgaagg ctcgaacacc gcctcggctg
    63721 aaattgaccg cgtatcgacg ctggaaatgt gccggattat caacgatgaa gataaaaccg
    63781 taccgcctgc cgttgatcgc gtcctgccgg atatcgccgc ggcgatcgat gttatccacg
    63841 cccaggtcag cggcggcggt cgtctgattt atctcggtgc gggaacatcc ggtcgtctgg
    63901 ggattctgga tgccagcgaa tgtccgccca cctacggcgt gaaaccgggt ctggtggttg
    63961 gtttgattgc tggaggcgaa tatgccattc agcacgcggt ggaaggagct gaagatagcc
    64021 gggaaggtgg tgttaacgat ctgaaaaata ttaatttaac ggcacaggat gtggtggttg
    64081 gcattgctgc cagcggtcgc acgccgtatg tgattgccgg actggaatac gcacgccagc
    64141 tcggctgccg cacagtggga atttcctgta atccggggag cgccgtttca accaccgctg
    64201 agtttgccat tacaccgatt gtaggtgccg aagttgttac cggttcttcg cggatgaaag
    64261 caggtacagc gcagaaactg gtgctcaata tgctttccac cgggctgatg attaaatccg
    64321 gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac caacgaaaaa ctgcatgtgc
    64381 gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc cgaacaagcg gaagcggcgt
    64441 taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat ggtgctgaaa aatctcgatg
    64501 ccgcagaagc taaaaaacgc ctggatcaac acggcggctt tattcgtcag gttttagaca
    64561 aggaataacc catggccaaa gagatcagca gtgaacttct gaacaccatt cttacccgtg
    64621 tcggcggacc gggaaatatc gccagttgtg gtaactgtat gacgcgcctg cgtctgggtg
    64681 tacatgacag ttcactggtt gatcccaata tcaaaacgct ggaaggcgtg aagggcgtca
    64741 ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg taaagcgcat cgtgccgcaa
    64801 aagcgatgag cgagctgctg ggcgatgcac cggtacagga tgccgcagaa atcgccgccc
    64861 agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt gcaacaattt ctcgccaaat
    64921 tcgccaccat cttcacgccg ctgatccccg gttttattgc cgccggtctg ctgctgggga
    64981 tagcgacgtt aattgccacg gtgatgcacg ttccggcaga tgctcagggc acgctacccg
    65041 acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt caccttccta gtgattctgg
    65101 tgggctataa cgccgcccag gcatttggcg gcacgggcgt caatggcgca attattgccg
    65161 cactcttttt gctcggttat aaccccgctg caaccaccgg ttactacgcc ggttttcacg
    65221 atttctttgg tctgcccatc gatccgcgcg gcaatattat cggcgtgctg attgccgcgt
    65281 gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat gccggacgat ctcgacatgc
    65341 tgctgacctc gttaatcacc ctgctgatca ccgctacgct tgcgtacctg atcattatgc
    65401 cgctgggtgg ctggttattc gaaggtatgt catggctgtt tatgcacctg aacagtaatc
    65461 cgttcggttg tgcggtttta gccgggctgt tcctgatcgc cgtggtattt ggcgtgcatc
    65521 agggctttat tcctgtttac ctcgcgttaa tggacagcca gggattcaac agcttatttc
    65581 ccatcctttc aatggcaggc gcgggccagg tgggcgcggc gctggcactc tactggcggg
    65641 cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc gattattccc ggcctgctgg
    65701 gcgttggtga accgctgatt tacggtgtca ccctgccccg catgaagccg tttgttaccg
    65761 cctgtttagg cggcgcggcg ggcggtttgt ttatcggatt gatagcctgg tggggtctgc
    65821 cgatgggctt aaacagcgcc tttgggccgt ctggtctggt ggcgctgccg ctgatgactt
    65881 ccgcacaagg catccttccg gcaatggcgg tttatgctgg cgggattctg gtggcatggg
    65941 tttgcgggtt tattttcacc acgctctttg gctgccgtaa cgtcaatctg gactgatatt
    66001 atgaaacgga caatgctcta tctttctctg ctggctgtta gttgtagcgt cagcgccgca
    66061 aaataccctg ttctgacaga aggtagttga tcaagtatat gcggcgttaa agcagtaata
    66121 aaaaggccag tcagtaatgt tactgcctgg cctttaatca atgaatcaga aacgcttaca
    66181 gcgccatcaa cttgtccagc gacggtgcga aataatagcc gccggttacc ggtttggtga
    66241 aacgcaacat cgcatcacgt ttgccatcgg tatcgccaaa catgctcagc agttgctgct
    66301 caatgttatg cagacgcgcg cagtaggcgc agaagtacag accgtgagtg ccactggcgg
    66361 tgccgtacgg caggctctgg cgaacaatct tcagcccttt gccatcttct ttcagatcaa
    66421 cgcgggtgag gtgagaggtt tccggacgtt cgtcgccgtc aatctcttcg ttggcctctt
    66481 tggtgcgccc gatcatcatc tcctgatcgt gaacgctcat ccggttgagc tgcttcaggt
    66541 tgtgctccca acgctgcaca aacacatagc tgccgccgga atccacgccg tctttgataa
    66601 ccgccacttc gcggcgcgtc tcttcacccg ccgggttttc tgtaccgtca acaaagccgc
    66661 tcaggtcacg ctcttccacc caacggaagc cgtggatctc ttctttcact tcaatgcagt
    66721 caccaaaggc ttccatcgcc gcctgagcga cagagaagtt tacgtcgtga cgcagagaaa
    66781 gaatgtggat caacacatcg aactgggtcg tcggcgcaag gcctttaccg tagcccggaa
    66841 aatctttcag ctcttctgcc ccaacgccgc cgctcagagc gcgccaggtg ttgttaccaa
    66901 aggcaaccac cgcaccaaga tgcgcgtccg ggaatttcgc ttcaaaagtt gccagtttgt
    66961 cggcaaatgt tttactggcc gcacgcaggg cgtcaacttc ccctttcacg ttggcttcga
    67021 tccaaatcgc cgcgcggcaa tgttctggca aaatgccact ctgaacctga gacattgttc
    67081 ctcctgaaaa taagaatgcc acgcaatccg tggcattgtt gagcgctatt ttacgtgttt
    67141 tttcgcgacg gcggtttgtt cagacgcaaa ttaacgccgc cagataattt tactgacttt
    67201 ccagtttttc agggtatcgt ccgaaggcat taacccttca ggaccacgcc attccccgct
    67261 gaactggtag ctaatatgtt gactgccttc ggctttacat tcgacggcac gattatcatc
    67321 accatcagct ttttggcaat tgccaaatgc tttgctgtaa aggtcgctaa acggtgtacc
    67381 gattttaaca ccggtgtcag caggaatatc gctatccagc acgtcaatgc gactgatcgt
    67441 gccttgatcg ccgttaatca ccatcgccac gttgtcgcct ttcatcactt caaaaaagcg
    67501 caccacgttg ccgttcgtgg ttttcattcc gctgcgcagg cgataatcgc catcaagcgc
    67561 atcagcaatg gcttgttctt gcagtggtgt ggacgccgtt aattcaccca cgccctgctc
    67621 gctcactttg gtggacgatc caaaccagtt ccacgggtta gcggcagacc agttaactga
    67681 cgacatcgtg gaacagccgg tcagcatcaa cggcatagcg cataacatta aacgcagcga
    67741 tttcatgtca cttcctttgg ttattcaata acgttgcttg gagtgcaaat tcaccaaaaa
    67801 gtgccgttat tctttttctt cattaaaaca agcgcgtaaa cgtcgattgg tcagcagcca
    67861 gattaacgcc acaatatccg ccactaccag cgccagaccg ataccgctaa cggattcacc
    67921 gttcagccac agccacggtt gccagcaaag taaaaccacc tgcgccaaca gcaacagaaa
    67981 atagagcaca tgccaggtgc gaggaaacga agcccgccga ccgctcaaca aaaacgccag
    68041 caccgccgga ataccgggaa tcagccccag ccagaaatta tcgtgatcgg gataaaacag
    68101 atttagcagc gcagtaccct gctcacgcga cgcaccggca atgacaaaca gcacccaggt
    68161 tcgcgcctga agcaatagca caagccagaa gagcaagggt aaacgcaggc gtccgtgcgc
    68221 atcataatgg acaggatgga actcagtact cttcatcttc aatcaaacgc ttacccagac
    68281 tcagcacgtc ggcgtgttca tatcccaggc gttcatacat tccgagcacc atgtcgttat
    68341 cttccggcac gttgatctga attttcgggc agccgcgagc aatcagcttt ttctccagcc
    68401 ggttaagcaa cgcattggca attccacgcc cacgaaactc tggatgcacg ccaagataat
    68461 aagcagaccc gcgatgcccg tcataaccgc ccatcaccgt tccgaccact tcaccgttta
    68521 cctcagcgac caaaaacaaa ctgacgtcat ggttcatctt acgctcgatg tccatttccg
    68581 gatcgttcca cggacgcagc aagtcgcaac gctcccaaag ggtgatgacc tcttcgaaat
    68641 cttcctggcg aaatacgcgt atctccatgg tattcgttac ctttttgcgg gttaaaaggc
    68701 tgattatggc gtgaacggtc gaattagcca atatctgacg aaaatcggtt gaaaaagtgg
    68761 cataatgggg agttgtcaac tattgaaatg aaaagtaaaa caattctcaa cagcaaaccg
    68821 tcgtaacgga ttacgcgata cgatataaca tctggaactt tattattaca actcaggccg
    68881 tatgagcact tttaaaccac taaaaacact cacttcgcgc cgccaggtgc tgaaagccgg
    68941 tttagctgcc ctgacgttgt caggaatgtc gcaagccatt gccaaagacg aacctttaaa
    69001 aaccagcaac ggacacagca agccgaaagc caaaaaatct ggcggcaaac gtgtcgttgt
    69061 tctcgatcca ggccacggcg gaattgatac cggagcgatc ggacgcaacg gttcgaaaga
    69121 aaaacatgtg gtgctggcga ttgctaaaaa cgtccgttcc attttgcgta atcatgggat
    69181 tgatgcgcgt ttaacgcgtt ctggcgatac gtttatccca ctttacgatc gcgttgaaat
    69241 cgcccataaa catggcgcag atctgtttat gtcaattcat gccgatggct ttaccaaccc
    69301 gaaagctgcc ggtgcttcgg tatttgccct ctctaaccgt ggggcaagta gcgcaatggc
    69361 gaaatacctg tctgaacgcg aaaaccgcgc cgatgaagtt gccggtaaaa aggcgactga
    69421 caaggatcac ctattgcagc aagtactgtt tgatctggtg caaaccgata ctattaaaaa
    69481 cagcctgacg cttggctcgc atattctgaa aaagattaag ccggtgcata aactgcacag
    69541 ccgcaacacc gaacaagccg cgtttgtggt gttgaaatcg ccgtcggttc cttcggtgct
    69601 ggtggaaacc tcgtttatca ccaacccgga agaagagcgg ctgttaggca cggcggcgtt
    69661 tcgtcagaaa atcgccacag cgattgctga aggcgtgatc agttatttcc actggttcga
    69721 caaccagaaa gcacattcga aaaagcgata agttatgaaa cccgacgcac accaggttaa
    69781 acagtttctg ctcaaccttc aggatacgat ttgtcagcag ctgaccgccg tcgatggcgc
    69841 agaatttgtc gaagatagtt ggcagcgcga agctggcggc ggcgggcgca gtcgggtgtt
    69901 gcgtaatggt ggtgttttcg aacaggcggg cgtcaacttt tctcacgtcc acggtgaggc
    69961 gatgcctgct tccgccaccg ctcatcgccc ggaacttgcc gggcgcagtt tcgaggcgat
    70021 gggcgtttca ctggtagtgc atccgcataa cccgtatgtt cccaccagcc acgcgaatgt
    70081 gcgttttttt attgccgaaa aaccgggtgc agagcccgtt tggtggtttg gcggcggctt
    70141 cgatttaacc cctttctatg gttttgaaga agacgccatt cactggcacc gcaccgcccg
    70201 tgacctgtgc cagccatttg gtgaagacgt ttatccccgt tacaaaaagt ggtgcgacga
    70261 ttacttctac ctcaaacatc gcaacgaaca gcgcggcatt ggcgggctgt tctttgatga
    70321 tctgaacacg ccagatttcg accactgttt tgcctttatg caggcggtag gcaaaggcta
    70381 caccgacgct tatttaccaa ttgtagagcg acgtaaagcg atggcctacg gcgagcgcga
    70441 gcgcaatttt cagctctacc gtcgcggtcg ttatgtcgag ttcaatctgg tctgggatcg
    70501 cggcacgctg tttggcctgc aaactggcgg gcgcaccgag tcgatcctga tgtcaatgcc
    70561 gccactggta cgctgggagt atgattatca gccaaaagat ggcagcccag aagcggcgtt
    70621 aagtgagttt attaaggtca gggattgggt gtaactccct cacccccatt cccgcatccg
    70681 ctgatgcaac gtcaacgacg gcttctcggc aaacagctgc tggtaatccg tggcaaattg
    70741 ccccagatgc cagaatcccc actgcatggc ggcgtctttt accgttgtgc tttgcgacca
    70801 cgggcttatc agttcgcggc gtacgacgtt caggcgaatg cgtttcagcc acgcgtttgg
    70861 gccaatgcct aaaatagcgt gaaacgcgtt ttgtagcgtg cggcgactga catgcagttg
    70921 attacacaaa tccagcaccg tcaccggttc ggacatgttt tccagcacat attcacgggc
    70981 gcgggaaagc aaccgacggt aactctgatg actgatgctt tccgccgtca ccattggttg
    71041 cgcttcttcc agcatcgccc ccatcgccat tagcaaatta tcccccagca cttttcgcac
    71101 tgcaggctga tggagatttt ccgggttctc gcaaaacgtc gccagcgcct gttggacaaa
    71161 gccccacagc gcggctttat gctgctcttt cacttccagc gccgactggc tacgcagcat
    71221 atgtaatacc cgatccggat tgtgcaaaaa gttagcctga caggtgatga catcttcaga
    71281 cagcactacg cccaggatcg tataatcgtc cggcgtactt aattcaaact cggttccacc
    71341 ggggcgcgtc gctatttctg cgcttcccag acattgcgaa ccgataaatc cctgctcacc
    71401 gcgcgtcgcc ggaatgccaa accagaacga gttcggccag accaggcacg actgacgcag
    71461 cgccagaccg gtgtattcac gaaaaacctg aatatcatcg agtagaattt ccgtaaattc
    71521 accatgaaac ttgcccggat gcagctgatc gtaaatctgc tgctaggcgg taatcgtcaa
    71581 agcatgttca tagacatccg ttgtctgtcg ttgatgaaca ttatccactt cgaccttcgg
    71641 cgtgagcttc aggttttcgg gtaagggttc atgataaaga tggtgcaaat tggctgtacg
    71701 ggtctttttc atgatgttaa tgccgggtgt tgtaggacac ccggcacctc cgacaggtta
    71761 atggggcttg agacgataac gactactgcg tttacgtaac gtcccggcag aaaagagctg
    71821 ttcgagcgca tttctggctt tttccagcgg ccagccaaag tgggcggcca cttctcctgc
    71881 cgtcattccc tgacatactg atgttaacag cgccaacaac tcgtcagcag attccgcgac
    71941 gataactggc gcgtcgggtt cctttacggg ttgcttcggc tggcggttaa agccagtaac
    72001 cagccactgg gtgtcatcgt ctggccgacc gatctcctta cggctgatga cacgcccggt
    72061 acgcatcgcg gcagcacaac ctgcgtccag cgccgcacga cacgccgcca gatcgccttc
    72121 caccaccagc gttaagcgac cagggtcaag cacttcgtga ctgagcagac gaacgttagc
    72181 tgctttgagc atggcatccg ctgcatctat cgcggcgacc atgccgtcca cttccagcaa
    72241 tcccagtgca ttgatcatcg gcaacctccg ttacgcacgc tggattggat tacgcgcgat
    72301 ttccagcact gcatcggtaa aggcgttaca ggctgcttta cacgcggcct ggctaccggt
    72361 taaaaatgct gccgagtagt tggtttcaga cggtggcggg acgtaggtca ccagctgtac
    72421 gtcggcagat ttcaacgcgg catcaatgcc gtaggtcgct tccagcggcg gtgccaccag
    72481 atacgccatc ggatcgccaa gcgtgatccc ggcggttgat gaaagataag aaccggtacg
    72541 cgaaaccaca tgcgccagga aggcggtgtt ttctgcgtca ttggcccact ggaaagctgc
    72601 gccattttca atatgtgcaa ccatcgcatc cagaccggca cgcacttccg ccgggtttgg
    72661 cccaccaagc ataatcagca cttcaccggc agtcggtgac gggccgtgtg ccgctcccgc
    72721 atacagcgag cgaccatata ccacttcgac catcgcttgt ttggtcgctt cgtcagccgc
    72781 aatgtaggtt acgtcgtcag aatcggcaga aatcagcccg aggctacgaa tatgcggcgg
    72841 caattttagt tcacgcgcaa attcagcgtt aacggaggca atcacccgca tggcggttac
    72901 cgacggtcga atcaaatcta aagctggcat gatgcctcct tatcgggtca tgttgatgcc
    72961 ggatgctttc tgctccagca tacgtttcgc caaatccaca atgacggcac agcagcacga
    73021 aagagaagca aaagtcagct acctggtaca ctaggcagcg ggtcagcaaa ggggggcaaa
    73081 tgcccctttt ttcatgtcag ataatcagat cattacctca ataaactaat aatcacatcg
    73141 cggttacaac cagtttgata gtgatagctc aaacccagac gtggcgctgg ctgcaagcaa
    73201 aataagccgc aaaaatgcga ccccatgttt actagccgca aaaatgcggt cctatttgcc
    73261 agaacctttt attattcaat tggttagtgc aagaaaaagc gtgattactg ttcaagaagt
    73321 atccagaaag gtcatgattt tgacgctgaa aaaaaatagg tgggcatttt tgcccactca
    73381 ctagccgcaa atttgcccac tcactagccg catttttgcg gcccaaagta ataaatacag
    73441 agaaataata aatacagatc agcgccaaga tttttcgtct tttcggcgga atcatgagac
    73501 tagtgcttac gtcctgataa cctccagagc agaccttaac ttctgtgcag caggactacg
    73561 ctcattgccg ctcggctagg tcaaatcctc acgataaagc tgttcggatt gtgaccataa
    73621 tgttaaagga ctctttaaat cattccttga cgaattaact agctggctta agcggaatag
    73681 catgcaggat aaaaacaaaa ccagcttagc agtggggaga gcttcgcttc gctcgtctcc
    73741 tggggccaat gctcaatgaa acgacgtaag gcacggagcg ctcaccatag gtagacacat
    73801 aaacatctga gagcgcacct tctatcgcgc tacgcgctca ataaaaacca acgacaccgc
    73861 cacactgcaa gaccaatggt ttttatcgcg tatacagacc agacaggaaa cagcagcaaa
    73921 gcagcgtata gccaggtgca gacagcacac tcacacgacg cacagcaggc ttaagtgatc
    73981 actcaggaaa ctttatcccc gccgccgatc accgcgaact atcatgaccg cgccaaattc
    74041 gaaaggaatg gcccatagaa cgcgatgaac acacataagc gctacactta cttaacgtcg
    74101 ccacagagcg attgagagcg cttaaaatcg atgcgatttc attaaatttt tctcactcgc
    74161 ctgaatggtg gcgatcagga ctgatcgcaa cattgccccg cctcgatatt gagcgcataa
    74221 tagatacata cattaagcgg aaaagagata agtttattgt gaaaatcagg gggttacata
    74281 tcatgcgcat atcagaacaa caacggcaga tcctcgacta tctggcaaaa aacgaatcaa
    74341 tcaccgcacg acaagccgca gatctcgttt atggtggcaa tgttacccgc gtccaggttg
    74401 acgccgcccg ccgctcactc atgacgatgg ttagtaatgg gctaatcagg aaacaaggcc
    74461 gcgctttcgt agctgaaaga catatcaatc acgcttatag ccaggacgtt gaagaattgc
    74521 gccgccagat cctttgggta ttcagcaaac agggtcagga gtatttcctt tctgaacacc
    74581 caaatttcgc cgccaaagag ttaacagtaa gcggattgtc gtgcgcactg gattatcttg
    74641 tcggtgaagg ttgcgttact tacaagcagc tatggcacgc cctgaatacg cttgtagaag
    74701 atggtttact cattgccacg cgtgagaaca tcgaatactg gataatgccg cgttcaggtc
    74761 gccgcaatca tgcacggtat atttcaggca acagatacag aacgccggac tttaatccga
    74821 cacaggaaga tcgggaccgt gccgaaatga tgtgcaggga acaggagaaa agaattatgg
    74881 ataagttatt ttcgatgcag tgaacacaag cgggcgcaat gcccgttttt attgccttat
    74941 tccacgcctg acgaatcaat ttgatcacac ctgagaaaat gcatagccag aagttaaggc
    75001 cgttatattg cgattgggag cgttttaaac ggtatcaatt taattaattc tgatgtggtg
    75061 atttgcaggc aaaaaaaacc cgccggagaa gcgggaaata gagacatatc gttgtagtta
    75121 ttaacagatc actttgaggt cgagggcggc taaccctcgc taatattatc ccgctattac
    75181 cttgcgggtg caaataatct tttattgctt tgatgattta ataaatagca ctttttgcta
    75241 acaaaaaccg ctgtcaatat atggccaaaa atgtcaacag aaaaaataag gtttataata
    75301 ttaagtatca catgggtaat gggttcaggg aatcggaatc caccgcctca cggaaattca
    75361 aaatttgctt cttctttcct ccgtgggcgg cttctttttc gtatcgaggt atttatgaaa
    75421 gcaataacat tagaacagtt cgcagcaatg gcgaatgtat cgaccagaac gctgaggcgc
    75481 tggctaaaaa aaccatcaac cggagtccct ttgccattta ccccacgcgg tactcgccaa
    75541 ttattctggg agcacgaatg caaagaatgg ttgtacagaa cagaacgata ataatcacgc
    75601 atcacatttt gaggtaaaca tcatgaccaa tatcgaaaga ttagaacgct gtgccgatat
    75661 tatgcgacgc agatggattt atgaccctga agcgggttta ttgtttagcc gtgaaacaaa
    75721 agacgttatc aaaggcagca tgaccaataa agggcatttg gttgtaacgg tacatgaagg
    75781 taaattcaaa gtgacactca cctatcagaa agcctgctat gtatacacat acggcgctta
    75841 tgacgagaca ttatatgaag tgcaccacgt taatttgaac aaacaggata accgcatcaa
    75901 gaatattcgc ctgatgccga aagaaaagca ccgccggata cataaaaacg tccgcaaact
    75961 tattcggctg ggccacacac aatggcttca accgcaatta attgaacaat cactataaac
    76021 acaggagtaa ataccatgca ttcattagac agttattttc agcgcacgac agcccctaaa
    76081 tcagccgccc aggaacgccg tgaggaattc catgagaaag tgatgcgttc tgctgattat
    76141 attgcggaca agttcgtgga aacggttcgt ccacttgttg atgaagtagc tgataaactt
    76201 cagtctgaaa tgccggaaga tatggaaggc actgcaaagc ggcgactcat ttgcgaatta
    76261 agtcgtcgtt ttggcgtgtc aataagcgct tttaaataat cgaaaaaagg ggggaaagat
    76321 aatggacgca tttgaggtac tggcaaataa aggcgctgaa ctggtagcac agcgtgataa
    76381 aaccgccaac gaaggggaac gcgcactgct aaataaacag attaaagcga tccgtatggc
    76441 ccagtttaaa ttaatcagta atgaagttat cgaaacaatt aaaccgcaga tcccacaaat
    76501 cgttgctgac gctattaaag ccgccggact ggagaaacgc attgccgggc taaaaacgaa
    76561 cgcagagaaa atggagatgt acaaacagag cggtgctccg aatcccgatg agtacttctc
    76621 accggatgtt gagtttatgg cgcaggttga ggaacgtctg ggtagctgtc tcaccgagga
    76681 acagcgtcgc tattttgatg gagtggatag ttcggcaggt attgacctga actcgtattt
    76741 cggtcgtgaa attgagcatt ttgatgccgc cccgtctatg ccggagccag aaccagaaat
    76801 ggccaccgat cctgaagctg agcaacggaa acgcgtattc aatgcgatag ggattcggta
    76861 ctgatcggat aaaagaaagc cgggaattac cccggctgac ttaacaatgt gaccaatgaa
    76921 caaatcggac aagacaaaca ggataagcag tctcgaaaca atgttcggga actatattag
    76981 cacaagtccg gagaaaatca tcatgttggc actacgcatc atgcagggca tcgctaagac
    77041 cctcgcagaa catgtcttag acctgaaaca ttcgccactg agcaaacagg ccatgaagcg
    77101 ccaaacgctt cgtctgtggg ctgaatattc gcttggaact atcaataaaa tcatcgacat
    77161 gaaatcgggg ccgagcaacc agagcgcgga ggaaatggag ttcatcaggc gtttaattct
    77221 cattcgcaga gacattcata gccagttaca cagcgtggga atcgatatta atgatgggac
    77281 gggcgattaa ttttcggaca agtactatgc gcgcgcgagg agctgaacat gaagcaagat
    77341 attgcagaca ggctggagat actcgaaggc cagcgggcag aagcgaaaca actacgtaag
    77401 caggccagaa gagcgcacag gaacaacgaa gcagagttac taaccaaata catcagtttc
    77461 accaattact gcatctacga gtgttacaag gaagatgcag aggactggct ggattcattg
    77521 ccagaacagt actgagcaga ataacaccac gtcatgttgt tctgaggtga gcatatcacc
    77581 tctgttcagg tggccaaatt tagtgtgcca cttcagcatc accgttgaaa tcacaataac
    77641 gatttttttg taaatatgct ctcacatatg taggaataga ccaggttggc attgaaacac
    77701 atctaccatg accataataa agtaattttt tgatatgttt atggttttat attaattaaa
    77761 atatatctaa atttgatagt tttaaaattg caaaatccag attgtgtttg taatgtttaa
    77821 tctgattgta ttgagtggcg tttattatga aaaataaatc aggtaattct tgttatagag
    77881 aagcagtcga tattcatagc tcacttgagc tttcatttat attgcataaa tatcatttgc
    77941 gtgtagatga gaagaaatct cgaaatgtat tcagcgccat acgtgatgag ctcatccttg
    78001 atggggattc acgacaaaac ttagctactt tctgtcaaac atggcttgat gatgaaatcc
    78061 gtgagttgct taatttagct attgataaaa acatgattta taaagatgaa tatccacaaa
    78121 tggcggaacc tgaaggtcgc tgtgtgcgta tgctttccga ttcgtggagt ttccccgatt
    78181 cgcgacacac cttaggttgc tcaaccattg gttcatctga agctgctatg ctcggccgct
    78241 tggcgttaaa atggcagtgg tgtaaaaaac gcgaagtaca aggtaaatca accgaacctg
    78301 atttgcgatc ctgtgcaaat atgctggcat aaattctgcc gttactgtga tgtgaaattg
    78361 tgtgaaattc cacttgatga tcatcacctg ataatgaatg ctaaagaggt tttgaagcat
    78421 gtcgatgaaa acactattgg tgtcgttacg actttaggta ttacttattt ttgtaaagca
    78481 gattgcgtta gtgtttttaa gataagtgtt aaagttaata ttagttttta tatttacatc
    78541 gtaattatat gtttctattt taaaaatgtt cgagagtata aaaatgtcta atgctaatat
    78601 cgcacccaaa agtaagaaaa tgacagtggg gacactcgct atcatgaata taactgctgt
    78661 agtaagtcta cgatttatac cttctgaagc agaatacggc ttaggtgcca ttttttacta
    78721 tacctttgct gcaattatgt ttttagtacc tgttgccttt gttgcggctg agctagctac
    78781 aacatatcca caaaaagggg gggcatttcg atggatagga gaagcatttg gggctcgctg
    78841 gggttttgta ggaatgctta tgacctggct acaagtcatc ccctactttc caacagtact
    78901 gacatttggt gcagtctcgg ttgcatttat tgatgttgat atgggggtgg cagaatcaat
    78961 tgccagtaat aaatggtata ttttattttt cgtactattt gtttactggg gagctatttg
    79021 tgttgcatta cgtggcgttg gtattttctc gcaggtatct aagtggtgcg gtattatagg
    79081 gacaattatt ccagctatta tccttgttat tcttggtttc tcctatctgt ggttcagcgg
    79141 taaaccagcg gtgatcccat taggttgggg tgatcttttc cctaatatag ctaacttcca
    79201 gaatattgtt cttgccgcca gcatatttct tgcttatgca gggatggaaa tgaatgcggt
    79261 ccatgttaac gatttagata atccaactaa aaaataccca attgcaatta ccatcgcttc
    79321 tataggtact attgcaatat tcctgctcag tactttaggg gtcgccttta ttattcctac
    79381 agataaaatt agtctaactc aaagtttgtt ggttgcctac gatatgctgt tcgaatgggc
    79441 tggagtgcca tggcttggct ctgtgatggc atttatgcta gcaattggcg tactgggtgg
    79501 ggtggttact tggattgcag ggcctaatac aggtgtatta gcgatcgcga aagctggtta
    79561 tttacctaag tttttccaaa aaaccaatcg tcatggaatg gggcaccatc taatgtttgt
    79621 acaaggtata attgtctcag ttctttctgt tacttttgtg ataatgcctt cagttcaagc
    79681 agcttttcag attttatcac agttaacagt tatgctctat ttagttatgt atatgctcat
    79741 gttcgctagt gctatctatc tacgacattc tcaacctgat acaaaacgcc catatcgagc
    79801 ccctgcttta tttttctggg caacacttgg attcttaggg tcttttctgg cttttgtatt
    79861 aagttttatt ccgcccgagc aaatcccaac tggtagtcca accagctatg ttctttattt
    79921 gttagtatta gttgccctat ttttattaat tccaatatta atttacgcct tccgaaaatc
    79981 tgactggaaa gatccaaact ctgatttcga accattttct tgggaaaaaa gaactagtaa
    80041 ttaaattttc cataagctac aaaagatgag gttaaatatg tttagtgctg aatctccaat
    80101 tggtgtaata gactcaggtg tcggtggatt gacatcggta aaggaaataa ttaatttgct
    80161 tcctggtgaa gatattatct attgtggaga taatatgaat gcaccttatg gcaaccgttc
    80221 tgccgatgat atcatcagtt tgactaaaaa aatgctgact tttttacagt caaggaatgt
    80281 caaactagtt gctgtcgcat gtaatactat atcctcaact ttggaatccg aagaatacag
    80341 cggatatgct aagagctttc cgtttcctat cttatccatc attgaacctg cagtagaaga
    80401 tgtgattcgc cagcaatata aaaatgttgg tattatcgct accgagttta ctattaaaac
    80461 ggggtgtcac aaagagctaa taaaaaaatt gaattcaaca ataaatgtat ttggagaacc
    80521 aagcaaaaat cttgcaatgt taattgagga aggtaatctt aatgctcctg caatcttgaa
    80581 tgacatcaaa aaacatgtta accatttaat ttccctccat ccagtaaatg aaattattct
    80641 cggctgtact cactatccaa ttgttcagaa tttttttgaa gcagttgcac cggatataaa
    80701 atttattaat cctgcacatg atcaggctat ctctattaaa aatcacttgg gacagctaaa
    80761 tttattaaac tcctcaaata taggaacttt gcaaataaat actagtggaa gtatggagat
    80821 ctacaaaacg gtactagggg aattgagcat tacgaaacct catacattca gtatcaggca
    80881 gttttaaatt tatccggtac accgtaaaat cccgtccttc tgaactgcac cccaaaagtt
    80941 ggacaccaac tgagtaaggt gcattttatg gacaaaccca gttacatact gggtagtgca
    81001 tccaattagt agaacatgtg tttttcgata aacgctccga tcactttttc gtggatctca
    81061 actgagcgtg agaagcagat tgttttacga cccaaccgct taatgcgggt gcgtagcgtc
    81121 agattattac gctcaatgcg ttgggtgaat attttgccgg tcagatgctt attcttcggc
    81181 acctcccggc catagctgcc ccagtcatcg ctggtcagca tgccgatgtt gaagggtgta
    81241 agcagtgcca gtagctcccg gcacgtttga tcggttcggg gaccaaaagt gtaggccagt
    81301 acaccgcctg ttttggtgtt gtacgcgtac ccgagccaat gttgccgggc tttactgcca
    81361 acgtagctcc attgctcatc aagctcgcag ataaacgcca catcagcatg ggcaacaggc
    81421 gaagaagtta ttcgctttgg cgcgagtttt ttaaagtccg gatgacggtg ttactaccaa
    81481 ttttcagtgt cctggcggta tcgcgaaccc cggcaccatt aaaggccatt tcagttatca
    81541 gctctttcat acccggctta cgtgcttgat aagtataagt gaactgaaac acacggtggc
    81601 agtcacggca gcgaagtctg tcacggcctt tagggttctg accatggcgg taaacctgag
    81661 ctgactgaca acggggacaa tgaatattaa cgctggtcat gagataacct caaaagcccg
    81721 tattatacat cagattcaac taattagagg catcacccat ccagcattat aaaacatact
    81781 aacaataact aaattataat aatcacaact tacaatcaaa taataaatgc ataagattaa
    81841 caatgatttt ttcgggttat atttatctaa aaaagcagaa cattatgtaa cttattattt
    81901 tctaagcgaa aatttattgt gcggtcagac taacaaactt gcgtaccacc cgaagaacat
    81961 gcgaagattt aagtattctt tcttttttgg tttttatttt tcattttctc ttttcttctc
    82021 tttccggttc tgttctggtt ttggtttttt cctttttatt tttattcttt cttcagggtt
    82081 atgctaaaac accaaaacat ttttcaccac aaaagcatcc aatcatcttg ccagaccaat
    82141 caccttaacc ggatacactg ccggatataa tcccactaac acccagacac caagcaccat
    82201 acctgataac taccgtattc aggaaggaaa tgcgagtgat ttcaccgtca ccggcacaac
    82261 cgatccgcca aaaagaggcg taccaatgtc gatatagtcc cccgcgcgga caatcacttc
    82321 gtcaatgact gccaacggga gttgttgtag ctgtgggcgc aacaacatgc ccagagcttt
    82381 accaaagtcc tgcccggcca ccaccagcag gggatgcgga ttcggaaaac gcgcgacgaa
    82441 atcgaccagc gcgttgatga ccgtcagaac cgcggcgtaa cgcacaggca gcgaggcggg
    82501 aagcgccagc acgtacacgt cagttttggg atcaagatcc agctgaagca gcgcctgttg
    82561 ccaggcactc accagatccg tttcatcaat cgggatcgcc accggcaaat tgcgcagcgg
    82621 cagttgtacg ccctccagcc agattgtgct gccagagagc gaaagggtat gtgcacccgc
    82681 gccaatcacc gtggagcgta cggtttgcgc cggaaactgc acattcatct cacgcaggcg
    82741 cggatggtca tgcagcgccg ttgccagaag tgggccaata tcggcaaaac agaacgggtt
    82801 ggcgggctgg tggcgataac attcgcccac gcctccagaa agcgtaatga tttcgggcgt
    82861 aacacctgcg ggcagcaaac cggtttgcat caatgcctgc gcgagcggcg aaagcgttcc
    82921 gtcaatcact tcgacaatca gcgccgccat ccgccgggta acctgcacca gctgcgcgcc
    82981 ggtcagcgaa cgggcgtcag tgcctgcacc gaagcactca tccacaatca tctgccccgg
    83041 tttatgagcg taaaccacgc gcccctggct gtcggtttcc agcaggcgac caccgacgtt
    83101 gagacaggca gtgccgctga tttttccggc atcgaacagg gcgtagttcg cggtgccacc
    83161 gccgatatcg atattcagta cccgacacag ccgttgttca gaaagggttt gcgccccggc
    83221 tccgtgaccg gcgatcacgg attcgaggtg cggcccggcg ctggcgacga caaaatcgcc
    83281 cagcgactgt ttttccttca tcactttttc catcagctgt gcgaccaggc tttcggtaaa
    83341 ctggccttcc ggcagctggg caatgatggt ttcgcggatg cgctggcttt cggttttgtc
    83401 ggctggcacg ctggcgggca cggacgccgt taccgggatc gattcatcac attcggtgat
    83461 ggtaaaaccc agtagatccg ccacttcacg ggcttccggg gtaataatgc tggcgcgcag
    83521 aaccacggac attgcctgtt cgccgcgcgc gtgtgcttca cgaatatcat tcgctgtgat
    83581 gagttttttc acctgcttcc tcctgtttgg tgagagatgc cagataatcc accagttgct
    83641 gcacgctttg cgggtcgtgg ctgtttagct cgaaaatcgg ctcttcgaaa cccgtctcaa
    83701 gcaataactt tcgcgttgcg gcgacgtcag catctggcat gtccgtttta ctgatgacgg
    83761 cgatttgccg tttgctgacg ccaatatcca gcaacccggc aggcaaccga ctttccgggt
    83821 catttgcgcc atggacatag atcagcatgt ctacatcctg tagcgtggta attaaggcgt
    83881 gataccagcg gggatggcta aaatattcac ccggcgtatc aatatcgccc ttatcattaa
    83941 attccacggc cagtgttttt ctggcgaggg tataattccc ctgcaacgca ttaaataacg
    84001 ttgtttttcc tgccccgacg ctacccacaa acgcaattcg tttcatgaat gcctcgtatt
    84061 aacttttggt catttcacaa agcgtataat ttaataagcg acctaaaccg ctgacggttt
    84121 gtgataacgc ttcttctact gcgcccaccg agccataaat caccaatgca ccgctgaagc
    84181 ggtcgagaaa accgatatgc acatcagcgg ctttcagcgc taaatcaccg gcgatcatcg
    84241 ccgtttcgcc gggagttagc gtcataatgc caattgcgcc cgcatccgga acgccgattt
    84301 tcttcgccag ttcctcaccg ggatgcgcga tgagatgcgc cagtgtgacc tgtttgccgg
    84361 gcacaaattc ctgaatgatg cgttctttat ccatgagtcg ccgcctgtga aaaactttcg
    84421 tgtatcgtga caatttttcg tcagagtaaa taacaaaatt cggcaagcgc gtttaaaagg
    84481 tgaggtagat caataagaat ggagagatat ttactgttat gaataaactt tcatatgcga
    84541 cgcatgaata taaataaaat aatatcgaga tattttatat actgattttt ttattattcg
    84601 caaactcagt aataagcttt gctaatatat ttcaggaaaa ccgcttaccc tgaatattca
    84661 gggtaagcgt gagagttaag aatgaattac agcggttggg tttgcgcttc aaccacggcc
    84721 agcgccacca tattgacgat acgacgcacc gatgcgatcg gcgttaacac gtgaaccggt
    84781 ttcgccacgc ccatcagcac cggaccgaca gtcacacctt ccgagctgga aacgcgcagt
    84841 aagttgtaac taatgcgggc agcttccatg ttcggcatca ccagaatatt ggcagaacct
    84901 ttcaaagggc tgtccggcat acggtcgttg cgaatcgctt ccaccagcgc tgcatcgccg
    84961 tgcatttcac catcaatcat cagatctggc gcacgatccc tgaccagttc cagcgcctga
    85021 cgcattttgc tcgacgacgg gcagtcagaa gaaccaaagt tggagtgcga caacagagca
    85081 acgcgcggct caataccaaa acgacggaca gtttctgccg ccatcaaggt gatctccgcc
    85141 agctcttctg cgtctggttc gtcattgacg taggtatcgg caataaaggt gttaccactc
    85201 ggcagcagca gcgcattcat tgcacctgcg gtgtgaacgc catcgcgata accaaagaca
    85261 tttttcacca cactaaaatg ttcatgataa tcgcccaccg taccgcagat cattgcatcg
    85321 gcttccccac gctgaaccat gatcgcgccg atcactgtcg ggttactgat cagcgcacgc
    85381 tgcgcctgtt cctgagtgac accgcgacgc ttcatgatct ggaagtattc ggtccagtac
    85441 tctttaaagc gcggatcgga ttcgttattg acgatttcaa aatcaacgcc cgctttgatc
    85501 tgcaagccca gtttctgaat gcgcatttcg atcacgttcg gacgaccgat aaggatcggt
    85561 ttcgccagtc ccagcgttac cagttcctga gtcgcatgca gaacgcgcgc ctcttcccct
    85621 tccggcagaa ctacgcgctt cggcgctttg cgagcctggg agaaaatcgg cttcataaac
    85681 aggttggttt tgtaaacgaa ctcagtcagc ttgtcgatgt agacgtcgaa atcagcaatc
    85741 ggacgcgtcg ccacgcccga ctccatcgcc gcttttgcga ccgcaggagc gatcttaacg
    85801 atcaagcgcg gatcaaacgg ttttggaatg atgtattccg gaccaaagct cagatcctga
    85861 acgccatacg ctgaagccac cacttcactc tgttctgcat gggccagttc tgcaatcgcg
    85921 cgtaccgccg ccagtttcat ctcttcgttg atggcggttg cgccaacgtc cagcgcgcca
    85981 cggaagatga acgggaagca aagaacgttg ttcacctggt tcgggtagtc ggaacggccg
    86041 gtacagatga tggcatccgg acgcacttct ttcgccagcg gcggcagaat ttccggttcc
    86101 gggttcgcca gcgccaggat cattggtgcc cgagccattt tcttcaccat ttcctgggtc
    86161 agcactttcg ggccggaaca gcccaggaaa atatccgcac cttcaatcac atcatcgagg
    86221 gtacgtttgc cgtcatccac caccgcatat gcggctttgg tttccgccat atttggctca
    86281 cggccctgat agataacgcc ttttgaatcg caaaccacga tgttatgttt ttgcagcccc
    86341 agcgctacca gcaggttcat acaggcgatt gctgcggcac ccgcgccgga aaccaccatc
    86401 cgcacgtcgg agatgttttt ctccaccacg cgcaagccgt tgaggatggc ggcagtgctg
    86461 atgattgccg tgccgtgctg atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt
    86521 ttctgttcaa tatagaaaca ttctggcgct ttaatgtctt cgaggttgat gccgccgaag
    86581 gtcggttcga gcgcggcgac gacttcaata aatttgtccg ggtcgagttc atcaacttca
    86641 atgtcaaata catcaatccc agcgaatttc ttaaatagaa cgcccttgcc ttccatcacc
    86701 ggtttgcctg ccagcgcgcc aatgttgcct aaccccagca ccgccgtacc gttagagatc
    86761 accgcaacca ggttaccgcg cgcggtatat ttgtaggctt ttagcgggtc tttttcgatt
    86821 tcaagacaag gtgcggcaac gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc
    86881 agaggcttgg ttggagaaac ctggattttc cctggaactg gaaattcatg gaaatcaagt
    86941 gcactttgtt ttaactggtc atccatttgg ttgttccttt cacgtaacgt tcacaaataa
    87001 agtgtgtggg caacagcccc tgcccacaac gtggcgcaca ttattaccct gccggagtct
    87061 acagactttg agcaagtcca aactctcacc attaatataa tgttttggta ataatcctat
    87121 aacactgatg ttacctgctt aatccagcaa taccatgcct gtctgctatg cttttttgat
    87181 gcgtttagcg aaatttctca gaagtgtgaa ttaacgcact catctaacac tttacttttc
    87241 aaggagtatt tcctatgaac gagttagacg gcatcaaaca gttcaccact gtcgtggcag
    87301 acagctgcga tattgagtcc attcgccatt atcatcccca ggatgccacc actaatcctt
    87361 cgctgttact caaggctgcc ggattatcac aatatgagca tttaatagac gatgctatcg
    87421 cctggggtaa aaaaaatggc aagacccagg aacaacaggt ggtcgcagcg tgtgacaaac
    87481 tggcggtcaa tttcggtgct gaaattctca aaatcgtacc cggttgcgtg tcaacagaag
    87541 ttgatgcacg cctctctttt gataaagaaa agagtattga gaaggcgcgc catctggtgg
    87601 acttgtatca gcaacaaggc gttgagaaat cacgcatttt gatcaaactg gcttcgacct
    87661 gggaaggaat tcgcgcggcc gaagagctgg aaaaagaagg tattaactgc aacctgacgc
    87721 tgctgttttc ttttacccag gcgcgggcct gtgcggaagc aggcgttttt ctgatttcgc
    87781 cgtttgtagg gcgtatttat gactggtatc aggcacgcaa gccgatggac ccgtatgtgg
    87841 tggaagaaga tccgggcgtt aaatcggtgc gcaatatcta cgactactat aagcaacacc
    87901 actatgaaac cattgtgatg ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa
    87961 ccggctgcga tcgactgact atcgcaccga atttgctgaa ggagctgcag gaaaaagttt
    88021 ctccagtggt acgtaaatta atcccgcctt ctcagacgtt cccacgccca gcacccatga
    88081 gcgaagcgga gttccgttgg gagcacaatc aggatgcgat ggcggtagaa aaactgtctg
    88141 aaggcattcg tctgttcgct gttgatcaac gcaaactgga agatcttctt gccgccaaac
    88201 tataaaccag ccacggagtg ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca
    88261 ctcagtatgg atgctgtaca aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg
    88321 gctgatattg ccgaagtgct gtggaacgat tttcttaaac ataatcctac cgacccaacc
    88381 tggtatgatc gcgaccgctt tattctttcc aacggtcacg cgtcgatgct gctctacagt
    88441 ttgctacatc tgaccggtta cgacctgccg ctggaagaac tgaagaactt ccgtcagttg
    88501 cattcgaaaa ccccaggcca cccggagatt ggctatacgc caggcgttga aaccaccacc
    88561 ggcccgcttg gacaaggttt ggcgaacgcc gtcgggctgg cgatagcaga gcgtacactg
    88621 gcggcgcagt ttaaccagcc agaccatgag atcgtcgatc acttcaccta tgtgtttatg
    88681 ggcgacggct gcctgatgga aggtatttcc cacgaagtct gttcgctggc aggcacgctg
    88741 ggactaggca agctgattgg tttttacgat cacaacggta tttccatcga cggtgaaaca
    88801 gaaggctggt ttaccgacga tacggcaaaa cgttttgaag cctatcactg gcatgtgatc
    88861 catgaaatcg acggtcacga tccgcaggcg gtgaaggaag cgatccttga agcgcaaagc
    88921 gtgaaagata agccgtcgct gattatctgc cgtacggtga ttggctttgg ttcgccgaat
    88981 aaagcaggta aggaagaggc gcacggcgca ccactggggg aagaagaagt ggcgctggca
    89041 cggcaaaaac tgggctggca ccatccgcca tttgagatcc ctaaagagat ttatcacgcc
    89101 tgggatgccc gtgaaaaagg cgaaaaagcg cagcagagct ggaatgagaa gtttgccgcc
    89161 tataaaaagg ctcatccgca actggcagaa gagtttcccc gacggatgag cggtggttta
    89221 ccgaaggact gggagaaaac gactcagaaa tatatcaatg agttacaggc aaatccggcg
    89281 aaaatcgcta cccgtaaggc ttcgcaaaat acgcttaacg cttacgggcc gatgctgcct
    89341 gagttgctcg gcggttcggc ggatctggct cccagcaacc tgaccatctg gaaaggttct
    89401 gtttcgctga aggaagatcc ggcgggcaac tacattcact acggggtgcg tgaatttggc
    89461 atgaccgcta tcgccaacgg catcgcgcac cacggcggct ttgtgccgta taccgcgacg
    89521 ttcctgatgt ttgttgaata cgcccgtaac gccgcgcgga tggcggcact gatgaaagcg
    89581 cggcagatta tggtttatac ccacgactca attggcctgg gcgaagatgg tccgacgcac
    89641 caggctgttg agcaactggc cagcctgcgc ttaacgccaa atttcagcac ctggcgaccg
    89701 tgcgatcaga tggaagcggc ggtgggctgg aagctggcgg ttgagcgcca caacggaccg
    89761 acggcactga tcctctcaag gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt
    89821 aaagagattg ctcgtggcgg ttatgtgctg aaagacagcg gcggtaagcc agatattatt
    89881 ctgattgcca ccggttcaga gatggaaatt accctgcaag cggcagagaa attagcagga
    89941 gaaggtcgca atgtacgcgt agtttccctg ccctcgaccg atattttcga cgcccaggat
    90001 gaggaatatc gggagtcggt gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca
    90061 ggtattgccg attactggta caagtatgtt ggtctgaaag gggcaattgt cgggatgacg
    90121 ggttacgggg aatctgctcc ggcggataag ctgttcccgt tctttggctt taccgccgag
    90181 aatattgtgg caaaagcgca taaggtgctg ggagtgaaag gtgcctgatg gtgatcgccg
    90241 gatgctgtat gttcccctca ccctaaccct ctccccagaa gggcgagggg acggtccgtg
    90301 cgctttttag ggccgcatct gcgattttgt ttaacgggtg atccacaacg tgggcctggc
    90361 gtctggccca tgccagttat cgcaggtagg ttctgcggcg taacgcacca ggcgaaaacg
    90421 ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg cttaatccgc gccctttcgc
    90481 taaggtcacc agttcacgcg atttctcatc aaatgttgcg ttcatcagtt ccagttcatt
    90541 cgtctcctgg ccgttgttga acggcaaacg caaccgaacc gggcgcgagg ctagcggctt
    90601 tttacgcgac acaatccatg ccaaatcaat ggtgttatag gcccctgcct cacagctaat
    90661 catcatcagt gctttatcat cggtcagcgc agtgacattc acctcacgac gtaacggatc
    90721 aagcgagcag cgcagaccat tcatccgcca gtttccataa tccagcaaat cgttacgttc
    90781 ttcgagtgag agtggcgtcg gcgttgggtt aaccaccgcg acctctttca gcgcaggcgc
    90841 gggcggtacg ctgagaggcg gttcgtcccc tttcttgatc cacgcggttt cactgccaac
    90901 gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt aagccgctca gagaaatggt
    90961 ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga attatctgca aaaacgcggt
    91021 gatggttgcc gtatcatcgg ttactaataa ccatggtgaa atccgccact tgtcaccact
    91081 taatgccaac ggctcaccat ctaatagcag ccgtggcgct atctcccctt cagacgcatc
    91141 cggcgacttc aacccaccgc gctcaatacg taaaacggca tcggtatgcg ccccagcgct
    91201 gcggctcagg gtcatcacca gtccattatg atcgcccgta ttgcgcgcca cacaaaaatt
    91261 ttgattatta caggtgacct gccagtcgga aaacgcccgt tgcgctggtg ccgcccacac
    91321 caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa atgcgatagc gcatggacgg
    91381 tacgacccca gaagttcaaa caaacagcac aagtcgtatc ttcgttgcgc aggtagcgcg
    91441 gctcaatcgg atttgtcgga ttgagccaat aataaacaac gaaatattat tgctattttt
    91501 cagtccatta aatgtgacat ttgcaaatag ttaagcaata acaccgtctt accgtcacgt
    91561 atctcgccgg ttttgatcat ctccagcgcc tggctgaacg gcagctcgag cacttcaata
    91621 tcttcatctt caacaccgcc accggcgtta gcgcgctgat tgtcactgta ttcggcgata
    91681 aaaaagtgga ttagctcagt cacaccgcca ggcgacatat acagttcaaa taatttgcgc
    91741 acttcgccca cttcatagcc cgtctcttca atcgcttctt tacgaatgca cacttccggt
    91801 tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct gcccgctttc attgccatta
    91861 acccaggtag cgacacggaa ctgacgaatc agaaccacgc tctttttctt cgcgttgtac
    91921 aggaggatcg tcgcaccatt accgcgatcg taaacttcac gtttatggcg gataacttcg
    91981 ccatctttgc gcgtgagatc gtaagtaatg ttgtgcaggg tgaaatagtt atcggagaga
    92041 attttgtctt taatgagggt gatttgttgc gtcataccga ctccacagcg cgaaatgaac
    92101 aattatatta cgctgtgaag tcgggtttgt ctgcgcaggc tatcagtgag atttgactga
    92161 ttttaccccc agccagtcaa taatcccctg tgccgcatga cgtccttctg ccattgcggt
    92221 aaccaccaga tccgcaccac gcacggcgtc gccaccggcg aagattttcg gattggtggt
    92281 ctggtaacgg tactggcttt ccacatccgc gatgatgcgg ccccatttgt ctaccgttac
    92341 accgtgcgac tccagccacg gcatcccgtg tggattgaag ccaaacgcca taatcaccgc
    92401 atcggctggc atgacaaatt cactgccctc aatcggtaca ggtcgccgac gcccctgggc
    92461 atccggctcg ccaagacgcg tgcgcaggaa acgaattccg caaacgtgac cttgctcatt
    92521 cagctcaagc gccaccggct ggacgttaaa ttcgaagttg gccccctctt cgcgggcgtt
    92581 cttcacctct ttcttcgagc ctggcatgtt agcttcatca cgacgataag cgcaggtgac
    92641 gttactcgcg ccgtggcgca gcgcagtacg cacacagtcc atcgcggtgt cgccgccgcc
    92701 cagtaccacg acgttaagtc cggcggtatt gataaacggc tcttccggta gctcttcgag
    92761 ccccatcacc tgtttagtgt tggcaatgag gaacggcagc gcgtcataaa cgcccggcgc
    92821 atcttcattg ggtaaacccg ctttcatgga acggtaagtg cctacgccaa cgaagaccgc
    92881 gtcgtattgt tccaaaagca aatccaaaga gacatcttta cccacttcac aattgagttc
    92941 gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt gccagcaggg atttatccag
    93001 tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt tctggatggc gatcgtacac
    93061 cgtcaccgcc acgccattgc gggtcagaac atccgcacag gccagccctg ccggacctgc
    93121 cccgataatc gccacccgct tgtccacttt ggtgacatga cttaagtcag gacgccaacc
    93181 tttcgccaac gcctgatctg aaatatagcg ttcaatgttg ccgatagtta ccgcgccgtg
    93241 ctcatcgcga atagtacagg caccttcaca caaacggtct tgcggacaaa cgcgtccggt
    93301 aatttccggc agggtgttgg tctggtgaga aagctcgacg gcggcgtcga tgtttccggc
    93361 tttcaccagt tcaatccact gcggaatatg gttatgcagc gggcaggtcc attcacaaac
    93421 gctgtgctcg ccgaacttaa ggcagcgcga ggcttcccgt tgtgcctggt cggcgcgaaa
    93481 tggcagataa atttcatcaa aaccggtttt gcgcgcttca atcgccagtt tatccggctc
    93541 gccacgcgcg ggcgttgcct gcatttgttc gactttactc attaccggca tttcttgtgc
    93601 agcggtactg gcatgccacg gttgatgctc ctgacgtgcg gtgcgcaagc ggcgggattt
    93661 cgccatgccg gagagtgcga cgtcagtgac cagttgcagc gcgtccgccg ggcaattctc
    93721 gacacaggca ggaccgtttt cgcgccctgc acaaaggtcg catttatgcg ccgtggcttt
    93781 tacttttcct gccgcgacgg gtgtcaggac gatttgcatc gtaccaaacg gacaggccac
    93841 cacgcaggat ttacagccaa tacacttttg ctgattgacc tgaatgctgt catcaacgtg
    93901 gctgattgcg ccattagggc agctacgggc gcagggcgca tcttcacaat ggtgacaggt
    93961 cactgcacta cgttgctgct gatgtttgat aaccgtaatt cggggatgaa aatggtgttg
    94021 gctcaggaca tgttgctcat cattgtgagc catgacacag gcgatttcac aagcatgaca
    94081 acccagacat tgctgactgt tggccataat aaaacgattc ataacgacct tcttttttgg
    94141 ttgtaaaaac cttattcttt atatgagtgt tgttattacc cgacttacag gggaaccggc
    94201 aatgttcatg tgcccagaat aagtaactat ttcgctataa actgtggcag atcaaataat
    94261 cccatcactg actaaattgc gtttcagcga actggaacat taatgatttt ttgtggagaa
    94321 gacgcgtgtg attgttaaac gacccgtctc ggccagtctg gcccgggcct ttttttacat
    94381 tgtgctgctg tcgattcttt ccacgggtat cgctctgcta actctggcga gcagtttgcg
    94441 cgacgctgag gctatcaata ttgccggatc gctgcgtatg cagagttacc gcctgggcta
    94501 cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc cagttgtttc agcaggcact
    94561 gcattcaccg gtattaacca acctcaacgt ctggtatgtg ccagaagcag taaaaactcg
    94621 ctatgcgcat ctgaatgcca actggctgga gatgaataat cggctcagca agggcgattt
    94681 gccgtggtat caggccaata ttaataatta tgttaatcag atagacctgt tcgtgctggc
    94741 tttacagcac tacgctgaac gcaaaatgct gctggtggtg gcgatttccc tggctggcgg
    94801 tatcggtatt ttcacgctgg tcttttttac tttgcgccgc atacgccatc aggtggttgc
    94861 cccgctgaat cagctggtta ccgccagtca gcgtattgaa cacgggcagt tcgactcgcc
    94921 gccgctggat accagtctgc cgaatgagct tggtctgctt gcaaaaacct ttaaccaaat
    94981 gtcgagcgag ctgcataaat tgtacctttc gctggaggcg tcagtagaag aaaagacccg
    95041 cgatctccac gagaccaagc gtcgtctgga ggtgttgtat cagtgcagtg ttcccaggcg
    95101 ctgaacacca gccagattga tgtgcattgt ttccgccata ttttgcagat tgttcgcgac
    95161 aatgaagcag ctgaatatct ggagctgaac gttggggata actggcggat tagcgaaggg
    95221 aagccaaacc ctgagctgcc aatgcagatt ttaccggtga caatgcaaga gacggtttac
    95281 ggcgaactgc actggcaaaa tagtcacgtt tcatcatcag aaccgctgct taacagtgtt
    95341 tcatcgatgc tgggacgcgg tttgtacttt aatcaggcgc agaagcattt tcagcagtta
    95401 ttgttgatgg aagaacgtgc gaccatcgcc cgcgaattgc acgactcgct ggctcaggtg
    95461 ctttcttact tacgtatcca gttgacgtta ctgaagcgtt cgataccgga agataacgcc
    95521 accgcacaaa gtatcatggc cgatttttcc caggcgttga atgatgctta tcggcagtta
    95581 cgcgagctgt tgactacttt ccgcctgacg ctgcagcagg cggatctccc ctccgcgttg
    95641 agggaaatgc tggatacgtt acaaaatcaa accagcgcta aactgaccct cgactgccgt
    95701 ctgccaaccc tggcactgga tgcgcaaatg caggtgcatt tgttgcaaat tattcgcgaa
    95761 gcggtgctga atgcgatgaa gcacgccaac gccagcgaaa tcgccgtcag ttgcgtcacc
    95821 gcgccggacg gcaatcacac ggtttatatc cgtgataacg ggattggtat cggtgaaccg
    95881 aaagaacccg aaggtcatta tggactgaat atcatgcgcg aacgcgcgga acggctgggc
    95941 gggacgctga ctttttcaca accttccggc ggcggcacgt tagtgagtat tagctttcgc
    96001 tccgcagagg gtgaggggaa ggggaacacg ccggctgagg tgtaaataat taccctcaca
    96061 ccggccctct ccctttaaag gcgagaggga aaaacgtgcc catattgacc tgtaagcgag
    96121 tgtgtcagca atctcatgat aatttacatt aactcctttt tttctccacg attggctcgt
    96181 accttgccgc tacagtgaag caagtcaagc ctacaacaat acgcagaaac acgaggtcct
    96241 cttttaatgg cgaatttctt tattgatcgc cccatttttg cctgggtgct gacaatcctg
    96301 ttgtgtctga caggtaccct ggcgattttt tcattgcccg ttgaacaata ccccgatctc
    96361 gcgccaccga atgtgcgagt gaccgctaac tatcccggcg catcggccca gacgctggaa
    96421 aacaccgtga cccaggttat cgagcaaaat atgaccggcc tcgataatct gatgtatatg
    96481 tcatctcaga gcagtggcac cggtcaggca tctgtcactt taagttttaa agcaggcacc
    96541 gatccggacg aagccgtgca gcaagtacaa aaccagctgc aatcagccat gcgaaagtta
    96601 ccgcaggcgg tgcaaaatca gggcgtgacg gtgcgtaaaa ccggcgatac caacattctg
    96661 accattgcct tcgtctctac cgatggctcg atggataaac aggatattgc tgattatgtt
    96721 gccagtaata ttcaggaccc gttaagccgc gtgaatggcg tcggggatat cgatgcctat
    96781 ggttcgcaat attccatgcg tatctggctg gacccggcga aactcaacag tttccagatg
    96841 acggctaaag atgtcactga tgccattgag tcacagaacg cgcagattgc ggttgggcaa
    96901 cttggtggta caccttccgt cgataagcag gcgctcaacg ccaccattaa cgcccagtca
    96961 ctgctgcaaa caccagaaca gttccgcgat atcaccttgc gggtgaatca ggacggctca
    97021 gaggtaaggc tgggcgatgt cgccaccgtc gaaatggggg cggagaaata cgattatctt
    97081 agccgcttca atggtaagcc agcctccggg ttgggggtaa aactggcctc cagcgcaaac
    97141 gaaatggcga cagcggagct ggtgctcaat cgtctcgacg agctggcgca gtatttcccg
    97201 cacggactgg aatacaaggt ggcgtatgaa accacctcgt ttgttaaagc ctccattgaa
    97261 gacgtggtga aaacgctgct ggaagctatc gctctggttt tcctcgttat gtatctgttc
    97321 ctgcaaaact tccgcgccac gctgataccc actatcgccg tgccggtggt gttgatggga
    97381 accttctccg tactttacgc cttcggttac agcgtcaaca ccttaaccat gttcgcgatg
    97441 gtgctggcga tcggtctgct ggtggatgac gccatcgtgg tggtggaaaa cgtcgaacgt
    97501 attatgagtg aggaaggact cactcctcgc gaagccacac gtaaatcgat ggggcagatc
    97561 cagggggcac tggtcgggat tgcgatggtg ctttcggcgg tatttgtacc aatggccttc
    97621 ttcggcggca ccaccggtgc catctatcgc cagttctcta ttaccattgt tgcggcgatg
    97681 gtgctgtcag tactggtagc gatgctcctc actccggctc tgtgtgccac gctacttaag
    97741 ccactgaaaa aaggtgagca tcacgggcaa aaaggttttt ttgcctggtt taaccagatg
    97801 tttaaccgca acgccgaacg ctacgaaaaa ggggtggcga aaattctcca ccgtagcctg
    97861 cgctggattg tgatttatgt cctgctgctt ggcggcatgg tgttcctgtt cctgcgtttg
    97921 ccgacgtcgt tcttaccgct ggaagaccgg ggcatgttta ctacctcggt acaattgccc
    97981 agcggttcaa cccaacaaca gaccctgaaa gtcgttgagc aaatcgagaa atattacttc
    98041 acccatgaaa aagacaacat catgtcggtg tttgccaccg ttggttctgg ccctgggggt
    98101 aacgggcaaa acgtggcgag aatgtttatc cgcctgaaag actggagcga acgcgacagt
    98161 aagaccggca cctcgtttgc cattatcgag cgtgcaacga aggcgtttaa ccaaattaaa
    98221 gaagctcgcg ttatcgccag cagcccacca gcaattagcg gtcttggtag ttctgcaggt
    98281 tttgatatgg agttgcagga ccacgctgga gcgggtcacg atgcgctgat ggcagcacgt
    98341 aatcagttgc tggcgctggc ggcggaaaac ccggagctaa cccgtgtgcg ccataacggc
    98401 ctcgatgaca gtccgcagtt gcagattgat atcgaccagc gtaaagctca ggcgctgggc
    98461 gttgatatcg acgatattaa cgacacactg caaaccgcct ggggttcgag ctatgtgaat
    98521 gactttatgg atcgcggtcg cgtgaagaaa gtctatgtgc aggcagctgc gccgtatcgc
    98581 atgctgccag atgacatcaa tctctggtat gtccgaaata aagatggcgg catggtgccc
    98641 ttctctgctt tcgcgacctc acgctgggaa acaggctcgc cgcgtctgga acgctataac
    98701 ggttattctg cggttgagat tgttggggaa gccgcaccgg gggtcagtac cggtacggcg
    98761 atggatatta tggaatcgtt agtgaagcag ctgccaaacg gctttggtct ggagtggacg
    98821 gcgatgtcgt atcaggagcg gctttccggc gcgcaggctc cggcgctgta cgccatttcc
    98881 ttgctggtgg tattcctgtg tctggcggca ttgtatgaaa gctggtcggt accgttctcg
    98941 gtaatgctgg tcgtgccgct gggggtaatc ggcgcgctgc tggcaacctg gatgcgcggg
    99001 ctggaaaacg acgtttactt ccaggtgggc ctgttaacgg tcattggttt atcggcgaaa
    99061 aacgccatcc tgatcgtcga gtttgctaac gagatgaacc aaaaaggcca tgacctgttt
    99121 gaagcgacgc tccacgcctg ccgtcagcgt ttacgcccga ttctgatgac ctcgctggca
    99181 tttatcttcg gcgtattgcc aatggcaacc agcacgggtg ccggttccgg tggtcagcat
    99241 gcggtgggta ctggcgtaat gggcgggatg atttcagcta ctattctggc tatttacttc
    99301 gtgccgctgt tctttgtgct ggtgcgccgc cgcttcccgc tgaagccgcg cccggaataa
    99361 gcaataaaaa aggcgacatg ccaatgtgtc gcctttttca actttccgat taagaacctg
    99421 ctcagcgggt tcttgctgtt tatactttgt ctcaggaatt acttacgaag cataacttcg
    99481 ataaagtctt tccagttccc cagttcacgt tcaatcataa caacctctct tataattatg
    99541 ggtattctac ggaaacaata taccgtggtg aagctaattt actcgattgc tgcgatgact
    99601 acctccgggg gacaaacctt atgtaaatac tatggtcctg cggtgataat ttgtatgtga
    99661 tacacagcaa catttcgaga tattcatacg gcatctaata ctgatttaat tctggttaaa
    99721 atacagacag ataacaagat gaatattctt aatgtttacg ttaaaaatgt ttaatattat
    99781 ttaatagttg ttaatttgaa tacttcgata atgttatatt ttctgataat catttgcagg
    99841 caaaatgttt tcacccttaa atgagtattt attctcataa atcgaaaaag gattcattat
    99901 ggttacactt tacggcatca aaaattgtga caccattaaa aaggctcgcc gttggctgga
    99961 agccaataac atcgactatc gttttcatga ttaccgcgtc gatgggctgg acagcgaatt
   100021 attgaacggt tttatcaacg aattaggctg ggaagcgtta ctcaacaccc gtggtacaac
   100081 ctggcgtaaa ctggacgaaa ctacccgcaa taaaatcacc gatgcggcct ctgcggcggc
   100141 attaatgact gaaatgcctg caattatcaa acgtccattg ctctgcgcgc ctggtaagcc
   100201 tatgctgctg ggtttcagtg attccagtta tcagcaattt ttccatgagg tgtagtctat
   100261 gtcgtgcccg gttattgagc tgacacaaca gcttattcgc cgcccttccc tgagtcctga
   100321 tgacgcagga tgtcaggctt tgttgattga acgtttgcag gcgatcggtt ttaccgttga
   100381 acgcatggat tttgccgata cgcagaattt ttgggcatgg cgtgggcaag gtgaaacgtt
   100441 agcctttgcc gggcataccg acgtggtgcc gcctggcgac gccgatcgtt ggatcaatcc
   100501 gccgtttgaa cccaccattc gtgacggcat gttattcggg cgcggtgcgg cagatatgaa
   100561 aggctcgctg gcggcgatgg tggtagcagc agaacgtttt gtcgcacaac atcccaacca
   100621 tacggggcga ctggcatttc tgatcacttc tgatgaagaa gccagtgccc acaacggtac
   100681 ggtaaaagtc gtcgaggcgt taatggcacg taatgagcgt ctcgattact gcctggtcgg
   100741 cgaaccgtcg agtatcgaag tggtaggtga tgtggtgaaa aatggtcgtc gcggatcgtt
   100801 aacctgcaac ctcaccattc atggcgttca ggggcatgtt gcctacccac atctggctga
   100861 caatccggta catcgcgcag cacctttcct taatgaatta gtggctattg agtgggatca
   100921 gggcaatgaa ttcttcccgg cgaccagtat gcagattgcc aatattcagg cgggaacggg
   100981 cagtaacaat gttattccgg gtgaactgtt tgtgcagttt aacttccgct tcagcaccga
   101041 actgactgat gagatgatca aagcgcaggt acttgccctg cttgaaaaac atcaactgcg
   101101 ctatacggtg gattggtggc tttccgggca gccatttttg accgcgcgcg gtaaactggt
   101161 ggatgcggtc gttaacgcgg ttgagcacta taatgaaatt aaaccgcagc tactgaccac
   101221 aggcggaacg tccgacgggc gctttattgc tcgcatgggg gcgcaggtgg tggaactcgg
   101281 gccggtcaat gccactattc ataaaattaa tgaatgtgtg aacgctgccg acctgcagct
   101341 acttgcccgt atgtatcaac gtatcatgga acagctcgtc gcgtgatgag tggttctgca
   101401 agaggaaata agcatggact ggctggctaa atattggtgg attctggtga ttgtcttttt
   101461 ggtaggcgtc ctgctgaacg tgattaaaga tctcaagcgc gtcgaccata agaaatttct
   101521 cgccaacaag ccggagcttc ccccgcatcg tgatttcaac gataagtggg acgatgacga
   101581 cgactggccg aaaaaggatc aaccgaagaa gtaagctgag tgctcaaccg taggccggat
   101641 aaggcgttaa cgccgcatcc ggcaatggtg aacgatccct gatgcgacgc tcacgcgtct
   101701 tatcaggcca ggtaatctgt cttttacatc atttcaatca catcatcatc gccaggctta
   101761 ccgccactta gcgcttggtc gaaataatgt ttcggaatgg tataacgcaa gtgatccagg
   101821 gcaaattgca tactgcgatt gtcgatcgca tggcctaaat cctccacgat atccagcgtc
   101881 acgtcaccac cggcactgat taacgcttcc tgcgccgcaa ctgcatgcgc cagatcgata
   101941 accggatctt caccaccatg aatcaagtga attgtcgtgg ctgtagacgc ggtctccggc
   102001 aggctcgcat agcgtccgtt aaaggcaatg acgcgcgaag caaggcccgg ttcggcttta
   102061 atgctctcca gcgccataat cgcgccttgt gagaaaccaa ttagcgccgt ggcgttagcc
   102121 cctactccgc tctgtttctg ccagtagcgt accgtttcaa taaacgtcgg catgatggca
   102181 tccacacgcg cctggcgatt atcttccgta ataccctgca ctgaaaacca ctgacgcccc
   102241 gccggattac cgctcggctc cgcgccaccg acactcacca ccagcgcatc aggaaacagc
   102301 ggtgcaaacc agctgcctat ttcccccatt gccaccgggt tatccccgac accgtggaaa
   102361 agcagtaaca actgttgtgc aggtttatcc gggctttgaa caacaaaatg gtcatgtttc
   102421 atggcgaact ccttaactga tgtcattaat tttacgccgc tgactcacaa tgaccatggg
   102481 agaaaattga aggagtcagt gaaaaaattg ccattgcgtt atgcgatcgc gcagacgctc
   102541 agtgcgaacc tcatttagtg cgaacagcgc ctgcgcggcc tcttcccgct ggcgaaccag
   102601 taacatcttg cggcctgaaa gtttaagtgt ggtacataac tgcgcgtcac tggcgttttt
   102661 ctgtaaacgc ccacgcagcg ccggtaatgc cagttcactg gtttgtagca gacgcaataa
   102721 gcaacctaac gatgttaata gcggacgatg agcgaaagca aaaccggcca gttcaagcca
   102781 gtcgtcgtca gaaaggatgg catcgtttag tggatcaaca ggaagcattt cgccattcca
   102841 ctgcgcgaga gcttgcgcat cgcgacgtaa acggtaatgc tcacgttcag ccagctgttt
   102901 acccgcatca ctcatcggta acagcgccat cgccgtatag caaccgctgc tggcttcccg
   102961 atgattaccc atccgcacca gcacaaaacc gcagcgttgc cagaaacgcc ataactcccc
   103021 agtgtaacca aaactcaccg aaagatagtc gaggtcatgc gtatattgca aagctccagc
   103081 aataagttgt cgccctgtgc cttcccgctg acgagccgga tgaactgcta tccggctaac
   103141 ccgccttccg cgcaatgtcg ccgccagtgg attgctgccg tgcgccgcca gcgactgggc
   103201 caccagatta ccccgcgggc gacgaaaacc tgcccatacc gcctgactga gttcttgaga
   103261 taatccgccc tcatccacca gccacaacgc cccggcaatc tcgttttcgc cagccgcctg
   103321 taaaaaatgt tgccctggtg catccatcat ccggcgtaaa tccagcggcg aggtccggta
   103381 gtgcgcacca gacaataact gataaacctt taacggcgtt tctggctcgc ttcgccataa
   103441 cgtttgttca aatgcggaaa tgacgatatt gccttgtggt gtatgggtga agttttcatc
   103501 gttaaaaacc agtgcctcac taaccatttt ttccagcggg catccctgtg cccagcggat
   103561 cggctgttgc agttcaaaac ggtgtaaatg cggaaagcga gcgcaaaatt tcagcaaaaa
   103621 accacgtccg gtaccttcgt agccctgcac cgtagtggtt aacaacgttc gaggaaaacg
   103681 cgataccagt tgatgcaaca acggcgcagg tatggctgcg gcttcgtcga ccaccagcca
   103741 gtcggcttgc tcatcgctgg ctaacaaggc atccggtgca ataaagcgaa acttctcgcc
   103801 cgcaaattgt gccagtacat ccgttgccgc ttttgcgggc gcggtgacaa tcgcactgcc
   103861 cgcaatacga gaaatgagtt gccctgccaa cgccgactta ccgcgcccac gcgcagccgt
   103921 taccgctgcc acgcccggcg gcatggtcag tagctgctgt aagagttgct gttgttctgg
   103981 ttgtggtgcg ccggtcgcgg ggtgccagtc agtacgggga gtaaaatgcg ccaacgagaa
   104041 cggctggttt tgccgccaga ggatagcgtc gttatccgcc gtaagtacgc gtttgagatg
   104101 ctggacaaaa tgcggcgtcg caatagggtc agggcaatca ctccagcgca gcgagtcggc
   104161 atcaggttgg ttttcccact cttcccatac agggagtaac aaaaccagcc agcttcccgc
   104221 tttcaacgtt ccgctaagtg ctgcaaaggc agcggcatca aagccgtggc gggcgtcgaa
   104281 taccgcatgc cggaactcgc gcccaagtaa agtttgtagc gccgagggtg aacagtggtt
   104341 ttcagcatct agctgcggcg aaatccacag ccagtcgcca ggtaaggcat cacgcaactt
   104401 aagagcatga tcaaaacacc aaccctcttc cccgctcaac accagcaagc ggcagatccc
   104461 ttcacgtttc atttgcgctg ttaatgtgta aagcgcagtc agttcagcca tccctgcccc
   104521 ggaaattaaa tgcttttacc aaaagtattg cattgtgccg gatcgccgct gtcgaaacca
   104581 cgtttaaacc agctgtagcg ttgctgagaa gtgccatgtg tgaaactgtc tggtactact
   104641 cgcccctgac tttgctgttg taaacggtca tcgccgatgg cctgcgccgc attcagcgcc
   104701 tcttccagat cgccagtttc cagaacgcct tgctgctgca tactatgccc ccagacaccg
   104761 gcaaaacagt cggcctggag ttccatacgc acagataagc ggtttacttc cgcctgcgtc
   104821 gcgttttgtt gcagttgacg aactttcggc tcgatgccta acagtttctg cacatgatga
   104881 ccgacttcat gggcgataac gtacccctgg gcaaaatcgc catccgcgcc aagtttgtct
   104941 ttcatgtcat catagaagga gagatcgata taaactgtgc catccgccgg gcaatagaac
   105001 ggccccataa tggactggcc cgccccgcag ccggtgcgcg tcattccacg gtacatgacc
   105061 agcttcggtt gctgataggt cttacccatc ttttcgaact gttgtcccca ggtgtcttcc
   105121 gtggttgcct gaatcaccga ggtgaatttt gcggcttcgt cttcattagg gctaattgac
   105181 cgcgttgatt gttgttgaga aaccggctgc ccggtcatca acccggttaa atcaacgcca
   105241 tagtagcctg caaccagcac gactatcagt aaaatcagcc cgcctttacc gctcggcaga
   105301 cggaaaccgg gaccgcccat tgacggaccg ccagagctgt tgcgcctgtc ttcaacattg
   105361 tcactttcac gtcgcccttg ccaacgcata gatacctcaa caatatattc attatagaaa
   105421 tgatcgtagg tggttcagag gaagattacc acaggaaagc gggatgaaat ggcggaggaa
   105481 tgagcgttca atgcatatat tccaagagcg gttgtcttgc cgtgccagct gcacggcaag
   105541 atgatgaaca gaaaaatcag tccagctgta cacccaggcg gcgggcgacg gcttcatagg
   105601 cttcgatcag gccaccgagg ctctggcgga aacggtcttt gtccattttc tccagtgttt
   105661 ctttgtccca caggcggctg ccgtccgggg agaactcatc gcccagtacc acttcacctt
   105721 tgtacagacc aaactccagc ttgaagtcga ccagaatcag accagcatca tcgaacagtt
   105781 ttttcagcac gtcgttcgct ttgtaggtca gctctttcat acgcgccagg ttctctttgc
   105841 tcacccagcc aaaggtttcg cagtaagatt cgttgaccat cggatcgtgc atggcatcgt
   105901 ttttcaggaa cagatcgaac agcggcgggt tcagctcaat accttcttcg attccaagac
   105961 gtttcactag agagccagca gcacggttac gcacgacaca ctcaaccggc accatatcca
   106021 gctttttcac cagacattcg gtatcggaga gcagacgctc catttgagtc gggatacccg
   106081 cttcagccag tttgctcata atgaagtagt tgaacttgtt gttcaccata cctttgcgat
   106141 caaactgctc aatgcgcgcg ccatcccctg ctgacgtatc attgcggaat tcgagcacca
   106201 acaggtccgg gttttccgtg ctgtatacgg ttttcgcttt accacgatac aactcagctt
   106261 gcttttgcat ctttatcact cctgggtgtg aattaacgtt ttaaaatctt ttgctgtctg
   106321 gtgtgccgga tgttttgtcg gatgcggcgt gaacgcctta tccgaactac gtccgacaaa
   106381 caaatttcgt gcgaattacc gctaaaatcg cgttttcctg ccgacgcaca cgtttgcgta
   106441 tcatatcaga aaaaagggcc ggatgattcc agccctgtat ttttacttgc taaacgcagc
   106501 ctggaagaca gctaccagcg cgtcgttctg actctgagtc agagtatgac ctttcggatc
   106561 gatgaactgt aggctgctgc ggttatctaa atcgccaacc tgcagtttat agtcaccgga
   106621 tgccaggcct ggatcgctcg cgcccagttc ctgccagtcg ctgtcagaca gcggcttata
   106681 agttacggcc atgttgccct gcgaacgggt gctgtcggtc actttcatgc ccactttttc
   106741 cagcgccgct ggcagacgtt gccaaaccac attgaacggc ccgcgtacga ccagcattgg
   106801 taaaccggtg tcatcagctg cactttgtac gtccatagtg gtggaggcac gattttgcgc
   106861 agcgttcgcg gcgtcagtgg cagatttatc cagaccggcg gaaataacgt tcatcatctc
   106921 cgtgctgtaa cgctgcatgg aagccgcgtc tgcaaccggt ttgcccgcct gttccaggtt
   106981 cagcagttta accgtaaccg cctgctgata accctgcggc ttaacagaga tttgataacg
   107041 accacgatac tgctcgtctt cgtccagacg gttccattgt acccaatcgg tggtcagtgt
   107101 ctgaccagca tcatcacgtt gggtgatggt gtagtttttc gcctgcagca cgctaaccac
   107161 ctgcggccac agagtattgc cacgaccatt ttccaccagc aatgaagcgg tatcgcccgt
   107221 gaactgggta cgcgcgccag aaaccagtgc caacggctgg gctggtggac gaatgtccag
   107281 cgccttaccg acagcaccac taccgttggt caccgggatt gcataatcac cggaggtcac
   107341 cggcaaaatc attccagccg gggcatgaag ctccgcaagc ggtgccgctt ccaggtaggc
   107401 ttcatcacca ctgacctgac gcttatagcg tgagtcagaa ctacaggcag cgagtaataa
   107461 aacaagcgaa acacccgcaa cctttgccag gcgcgacttt tgaacagagt aagccatcaa
   107521 atctccctaa actttacagc aaaccggcat gcttaagcgc cgctctgacc gtctcacgac
   107581 cactgtcggt gattggtgtc attggcaggc gcagcgtatc ggtcgccaca agacccagtt
   107641 ccttacatgc ccatttcacc gggattggat tgggttcgac aaatagtttg ttgtgtaatg
   107701 gcatcagacg ctgattaata acgcgtgcct cggcaaaatg cccttctgct gccagtttgc
   107761 acatctgggc catatcacgc gctgcgacgt tagccgtaac ggaaataact ccatgaccgc
   107821 ccaattgcat gaagtccagc gcgctcgcat catcgccgct cagcagaaca aaatcatctg
   107881 aaaccagctc tttgatctgg tttacacgcg ttaagttccc tgttgcctct ttgattccga
   107941 taatattttt tactttcgcc agacggccca ccgtttccgg gagcagatcg cagccagtac
   108001 gggacggcac attatacaga atttgcggca ggtcagtatg ctcagcgatg gctttgaaat
   108061 gctgatacaa accttcttgc gacggacgat tgtagtaagg ggttaccgtc aggcagccga
   108121 cgataccact gtcattgaag cgctgcgtca ggctaatggc ttccgcagta gcgttagcgc
   108181 cggtcccggc aattaccgga atgcgcccat cagccagatc cagcgtcatc atcaccacat
   108241 cagcatgttc gtcatgattt aaggtagcgg actcgccagt ggtgccaaca gaaacgatcg
   108301 ccgaagtacc gctggcgaca tgataatcaa tcagtttttt caagctagcc cgacagacat
   108361 tacctttttc atccatcgga gtaacaatcg cgacaatact tcccgtgaac atgggccatc
   108421 ctctgtgcaa acaagtgtct caatggtacg tttggtatgg cattaaaagc aagcagacag
   108481 aaccgttctg attgttgtat gcatgatttt tttatgcttt ccttaagaac aactcacccc
   108541 ttaaaggaat aaccagtttg acactgtcat cgcaacatta tctggtgatc actgcgttgg
   108601 gtgccgatcg ccctggaatt gtgaacacca tcacccgtca tgtcagtagt tgcggctgta
   108661 atattgaaga cagtcgcctg gcgatgctgg gagaagagtt cacgtttatc atgctgcttt
   108721 ccggttcatg gaatgccatc actctgattg aatcaacgtt accgttgaaa ggtgccgaac
   108781 tggatctttt aatcgtgatg aagcgcacga cggcgcgtcc gcgtccgcca atgccagcat
   108841 ctgtctgggt tcaggtcgat gttgcagact ccccgcattt aattgaacgc ttcacagcac
   108901 ttttcgacgc gcatcatatg aacattgcgg agctggtgtc gcgcacacaa cctgctgaaa
   108961 atgaacgggc tgcgcagttg catattcaga taaccgccca cagccccgca tctgcggacg
   109021 cagcaaatat tgagcaagcg ttcaaagccc tatgtacaga actcaatgca caaggcagta
   109081 ttaacgtcgt caattattcc caacatgatg aacaggatgg agttaagtaa tgaatccact
   109141 gaaagccggt gatatcgcac cgaaatttag cttgccggat caagacggag aacaagttaa
   109201 tttgaccgac ttccagggac agcgtgttct ggtttatttc tacccgaaag ccatgacccc
   109261 cggctgtacc gtacaggcct gcggcttacg cgataacatg gatgagttga aaaaagcggg
   109321 cgttgatgtg ctgggtatca gcaccgataa acccgaaaaa ctctcccgtt ttgcggaaaa
   109381 agagctgctt aactttacgc tcctgtctga tgaggaccac caggtgtgcg aacaattcgg
   109441 cgtctggggt gaaaagtcct tcatgggcaa aacctacgat ggtattcatc gcatcagctt
   109501 cctgattgac gctgatggca aaatcgaaca tgtctttgac gatttcaaaa ccagcaatca
   109561 ccacgacgtt gtgctgaact ggctgaaaga acacgcctga ttactttgct ccattccgtg
   109621 ctggctgcgc ttgcggccag catacctcac ttctcgtgat caagatcaca ttctcgcttt
   109681 cccctgcgac acgggtgtcg aatccatttt ttgctgaacg ttaatgacca tcatttttgt
   109741 accgttcaga atccagttaa tacataactt attgaataga ttgagttaat cagaatggca
   109801 tcctttatac aatatgaaat gcaatgtttc atatcatttt caaggagccg acatgaaccg
   109861 ctttgtggtg gccgaaccac tgtggtgtac aggatgtaat acctgtctcg ctgcctgttc
   109921 ggacgtgcat aaaacgcaag gtttacagca acacccgcgc ctggccctgg cgaagacgtc
   109981 aacaatcact gcccctgtcg tgtgtcatca ctgtgaggaa gccccttgcc tgcaggtctg
   110041 cccggtcaat gccatctctc agagggatga tgcgatccaa ctcaacgaaa gcctctgtat
   110101 tggctgcaag ctttgcgccg tggtctgccc atttggcgca atcagcgctt caggaagccg
   110161 tccggtgaat gcccatgcgc aatatgtttt tcaggctgaa ggctcactca aagacggcga
   110221 agaaaacgtg ccgccccaac atgcgttgct gcgctgggaa ccgggtgtcc agaccgtcgc
   110281 ggtgaaatgc gacctgtgtg atttcttgcc agaaggtccg gcctgcgttc gcgcttgccc
   110341 gaatcaggcg ttacggctga tcaccgacga tagcctgcaa cgtcagatga aagaaaaaca
   110401 gcgccttgcc gcaagctggt ttgccaatgg cggggagtct cccctttccc tcactcagga
   110461 gcaacactaa tggatgccct gcaattatta acctggtcgc tgatcctcta tctgtttgcc
   110521 agtctggctt cgctgttttt actcggtctg gacagactgg ctattaagct ttccggcatc
   110581 acatcgctgg tgggcggcgt gattggcatc atcagcggaa ttacgcaatt acatgctggt
   110641 gtaactttag tcgcccattt tgccacccct tttgactttg ccgatttaac cctgcgaatg
   110701 gatagcctct cggcatttat ggtgctagtt atctccttgc tggtggtggt ttgttcgctc
   110761 tattcattga cttatatgcg cgaatacgag ggcaaaggcg cggcggcgat gggcttcttt
   110821 atgaatcttt tcatcgcatc gatggttgcc ctgctggtga tggacaacgc tttttggttc
   110881 atcgtgctgt ttgaaatgat gtcgctgtct tcctggtttc tggtcattgc caggcaggat
   110941 aaaacgtcga tcaacgctgg catgctctac ttttttatcg cccacgccgg atcggtgctg
   111001 attatgatcg ccttcttgct gatggggcgc gaaagcggca gcctcgattt tgccagtttc
   111061 gcacgctttc actttctccg gggctggcgt cggcggtgtt tctgctggcc tttttcggtt
   111121 ttggcgcgaa agccgggatg atgccgttgc acagctggtt gccgcgcgct caccctgccg
   111181 caccatcgca cgcttcggcg ttgatgtctg gcgtaatggt caaaatcggt attttcggca
   111241 tcctgaaagt agcgatggat ctgctggcgc aaacgggttt gcctctgtgg tggggcattc
   111301 tggtgatggc gatcggcgca atctccgcgc tcctgggcgt gctatatgcg ctggcggaac
   111361 aggatatcaa acggctgctg gcctggagta ccgtcgaaaa cgtcggcatt attttgctgg
   111421 cagtcggtgt ggcgatggac ggtctgtcac tgcacgaccc gctgctcacc gtggttggac
   111481 tgctcggcgc actgtttcat ctgctcaacc atgcgctgtt caaagggctg ctatttctcg
   111541 gcgcggacgc gattatttcg cgtttgcata cccacgacat ggaaaaaatg ggggcactgg
   111601 cgaaacggat gccgtggaca gccgcagcat gcctgattgg ttgcctcgcg atatcagcca
   111661 ttcctccgct gaatggtttt atcagcgaat ggtacacctg gcagtcgctg ttctcactaa
   111721 gtcgtgtgga agccgtagcg ctacaacttg cgggtcctat tgctatggta atgctggcag
   111781 tcactggtgg gctggcagta atgtgcttcg tcaaaatgta cggtattact ttctgtggtg
   111841 cgccgcgcag tacacacgct gaagaggcac aggaagtgcc aaatacgatg atcgtcgcca
   111901 tgctactgct cgcggcactc tgcgtattaa ttgcgcttag tgccagttgg ctggcaccga
   111961 agataatgca tattgcccat gcgtttacca atacccctcc cgtcactgtc gccagcggaa
   112021 tagcacttgt acccggcacg tttcatacac gggtcactcc ttcattactg ttgctgttac
   112081 tactggcgat gcctttgctg ccaggccttt actggctgtg gtgtcgttcg cgccgcgcag
   112141 cgtttcgtcg cacaggagat gcctgggcat gcggctacgg ctgggaaaac gcgatggctc
   112201 cgtcaggcaa tggcgtgatg cagccgctgc gtgtggtctt ctctgcgtta tttcgtctac
   112261 gacaacagct cgaccctacg ctgaggctaa ataaaggtct tgcgcacgtc accgccaggg
   112321 ctcagagcac agaacccttc tgggatgagc gggtgatccg ccccatcgtg agcgccaccc
   112381 aacggctggt caaagaaata cagcatctgc aaagcggcga ctttcgtctc tattgcctgt
   112441 atgtggtcgc cgcactggtt gtgctgctaa tcgctattgc cgtctaagga aatcaccatg
   112501 agacaaactc tttgcgacgg atatctggtc atttttgcgt tagcacaggc cgtgattctg
   112561 ctgatgctaa ccccactttt tacgggtatt tcccggcaga tacgcgcgcg tatgcactcc
   112621 cgccgcgggc cggggatctg gcaggattat cgcgatatcc acaaactgtt taaacgccag
   112681 gaagttgcgc cgacatcttc aggtctgatg ttccgcctga tgccgtgggt attaatcagc
   112741 agcatgctgg tgctggcgat ggccttacca ctatttatta ccgtttcccc ttttgcgggc
   112801 ggcggcgatc tgatcaccct tatctatctt cttgccctgt ttcgtttttt ctttgctctt
   112861 tccgggctgg ataccggaag tccgtttgcg ggagtcggtg ccagtcgcga gttgacgctc
   112921 ggcattctgg tcgaaccaat gcttattctc tcactgctgg tattggcgct gatagcaggt
   112981 tccacgcata tcgagatgat cagcaatacg ctggctatgg gctggaactc gccgctaacc
   113041 accgtactgg cgttactggc ctgtggtttt gcctgcttca ttgagatggg aaaaattccc
   113101 tttgatgttg ctgaagcaga acaggaatta caggaaggcc cgctgaccga atattccggt
   113161 gccaggctgg cgctagcgaa atgggggctg gggctgaaac aggtcgtgat ggcagcactg
   113221 tttgtggccc tgtttctgcc ctttgggcgc gcgcaagaac tttctctcgc ctgcctgctg
   113281 acttcacttg tcgttacgct gctcaaggtt ttgctgattt ttgtactggc ctcaatcgca
   113341 gaaaacacgc tggcacgcgg gcgtttttta ctcattcacc atgtgacctg gcttggcttc
   113401 agccttgctg cgcttgcatg ggtcttttgg ttaaccggtc tgtaaggagc actgacggaa
   113461 tatggaaaat cttgctctga cgacgttaat gctgcctttt atcggcgcac tggtcgtttc
   113521 gttttcgcca caacgtcggg ccgccgaatg gggggttttg ttcgccgcgc tgaccacgct
   113581 gtgcatgttg tcgctgatct ccgcgtttta tcaggccgat aaagttgccg tcacgttgac
   113641 gttggtcaac gtgggggatg tggcattatt tggcctggtc attgatcgcg tgagtacgct
   113701 gattctgttt gtggtggtgt ttctcggttt gctggtcacg atctactcca cgggttatct
   113761 gacggataaa aatcgcgaac acccgcataa cggcacgaat cgttattacg catttttgct
   113821 ggtgtttatc ggcgcgatgg cgggactggt actctcctcg acgctgctcg gtcagttgtt
   113881 gttttttgaa attacgggcg gctgctcctg ggcgttgatc agttattacc agagcgataa
   113941 agcgcagcgt tcagcactaa aagcgttgct tatcacccat atcggttcgt tggggttgta
   114001 tcttgccgcc gccacgctgt ttttgcagac cggaacgttt gtgcttagcg cgatgagcga
   114061 gttacatggc gacgcacgtt atctggttta tggcggcatc ctgtttgccg cgtgggggaa
   114121 atcggcccag ctaccgatgc aagcgtggct accggacgca atggaagcgc caacaccgat
   114181 cagcgcctat ctccacgccg catcgatggt gaaagtgggc gtttacattt ttgcccgcgc
   114241 cattatcgac ggcggcaata tcccgcatgt gattggcggc gttggcatgg tcatgacact
   114301 ggtcaccatt ctttatggct ttctgatgta tttgccacag caggatatga agcggttgct
   114361 ggcctggtcg accatcactc aacttggctg gatgttcttc ggcttgtcgc tctccatctt
   114421 cggctcgcgg ctggcgctgg agggtagcat cgcctacatc gtcaaccacg cgttcgctaa
   114481 aagcctgttt ttccttgtag caggtgcgct gagttacagc tgcggcacgc gcttgttgcc
   114541 gcgtctgcgt ggcgtattgc acaccctgcc gttgccaggc gtgggtttct gcgtggcagc
   114601 gctggcaatt accggtgtgc cgccgttcaa cggcttcttc agtaaattcc cgctgtttgc
   114661 cgccggtttt gcgttgtcag tggagtactg gatcctgctg cccgccatga ttctgctgat
   114721 gattgaatcg gtcgccagtt tcgcctggtt tattcgctgg tttggtcgcg tcgtgccggg
   114781 caaaccgagc gaggccgtcg ccgatgccgc accgctgcca gggtcaatgc gcctggtgtt
   114841 gattgtactg attgtgatgt cgctgatttc cagcgtaatc gccgcgacct ggttgcagta
   114901 aggagatgat gaatgaccgg ttctatgatc gtaaataatc tggcgggact gatgatgctg
   114961 acatcgctgt ttgtgattag cgtcaaaagc tatcgcctgt catgcggatt ttacgcctgc
   115021 cagtcgctgg tgctggtgtc tattttcgcc actctctcgt gcctgttcgc cacagagcaa
   115081 ctgctgatct ggtccgccag cgcctttatc accaaagtgc tgctggtacc gttaatcatg
   115141 acttacgctg cacgaaatat tccccagaac atcccggaaa aagcgttatt cggtccggca
   115201 atgatggcac tgctcgcggc gttaattgtc ctgctttgcg catttgtcgt tcagcccgtg
   115261 aagctaccga tggctaccgg gctgaaaccg gcgctggcgg tagcgttagg tcattttctg
   115321 cttggcctgc tgtgcattgt cagccaacgc aatatcctgc ggcaaatttt tggttactgc
   115381 ctgatggaaa acggctccca tctggtgctg gcgcttcttg cctggcgagc accggaactg
   115441 gtggaaatag gtatcgctac cgacgccatc ttcgccgtca ttgtgatggt gttactggca
   115501 agaaaaatat ggcgtaccca cggcacgctg gacgtgaaca acttgaccgc gctgaaggga
   115561 taatgagatg agttattctg tgatgttcgc tttactcctg ctcacgccgc tgcttttttc
   115621 gctgctctgt tttgcctgcc ggaaacgggg gctctctgcg acttgtacgg tgactgtatt
   115681 acatagctta gggatcacac tgctgctgat tctagcactc tgggtggtcc aaactgccgc
   115741 tgatgcagga gaaatattcg ctgcgggact gtggcttcat attgatggtc tgggcggttt
   115801 gttcctcgcc attcttggtg tgattggctt tctcaccggt gtttactcga ttggctacat
   115861 gcgtcatgaa gtggcacacg gcgagctttc acccgttaca ctgtgcgatt actacggttt
   115921 cttccatctg tttttgttca ccatgctgct ggttgttacc agcaataacc tgattgtgat
   115981 gtgggcggcg atcgaagcca ccaccttaag ctcggcgttt ctggtaggca tttacggtca
   116041 gcgttcatcg ctggaatcag catggaagta catcattatt tgtactgttg gtgtcgcttt
   116101 tggtctgttc ggtaccgtgc tggtatacgc caacgccgcc agcgttatgc cgcaggcaga
   116161 aatggcgata ttctggagcg aggttcttaa gcaatcgtcc ttgcttgacc caacattaat
   116221 gctgttggcc tttgtgtttg tgctaattgg ctttggtacc aaaaccgggc tatttcccat
   116281 gcacgcctgg ctgccggatg ctcacagtga agcgccgagt ccggtcagcg ccctgctctc
   116341 cgccgtattg ctgaactgcg cgctgttggt gctgattcgc tattacatca ttatttgcca
   116401 ggccatcggc agcgatttcc ccaaccggtt gttgctcatc ttcggcatgt tgtcggttgc
   116461 cgtggcggca tttttcattc tggtacagcg ggacattaag cgtctgctgg cgtactccag
   116521 cgtggagaac atggggctgg tcgcggtggc gctaggcatt ggcgggccgc tgggaatttt
   116581 tgccgcgctg ctgcacacct taaaccacag tctggcaaaa acgctgctgt tctgcggttc
   116641 cggcaatgta ctgctcaagt acggcacgcg cgatctcaac gtcgtctgtg ggatgctcaa
   116701 aatcatgcca tttaccgccg tgctgtttgg cggcggtgcg ctggcgctgg cgctggcgct
   116761 ggcagggatg ccgcccttca acatttttct tagcgaattt atgaccgtta ccgcaggact
   116821 ggcacgtaat cacctgctga ttatcgtcct gctgttattg ctgttaacgc tggtgctggc
   116881 gggcctggta cggatggctg cgcgggtgtt aatggcgaaa ccgccgcagg ccgttaaccg
   116941 gggtgatctc ggctggttga ccacctcgcc aatggtgatt ctgctggtca tgatgctggc
   117001 gatgggaacg catattccac aacctgtcat caggatcctg gcgggcgctt ccactatagt
   117061 cctctcaggg acgcacgacc tgcctgcaca acgtagcacc tggcatgatt ttttgccttc
   117121 aggcaccgca tctgtttcgg agaaacacag tgaacgttaa ttcatcgtca aatcgtggcg
   117181 aagcgattct cgccgccctg aaaacgcagt tccccggcgc ggtgctggat gaagagcgac
   117241 aaacgcctga acaggtcacc attacggtga aaatcaatct gctgcctgac gttgtacatt
   117301 atctttatta tcaacatgat ggctggcttc cagtcctgtt tggcaacgac gagcggacac
   117361 ttaacggtca ttacgcggtt tattatgccc tttcaatgga aggggccgaa aaatgctgga
   117421 tcgtggtgaa ggcactggtc gatgccgaca gtcgggagtt tccgtcagtc acaccgcgcg
   117481 tccctgccgc ggtctggggc gagcgagaaa ttcgcgatat gtacgggctg attccggttg
   117541 gcctgccgga tcagcgtcgc ctggtgttgc ccgatgactg gccggaagat atgcatccgc
   117601 tgcgcaaaga tgcgatggat tatcgactgc gccctgaacc gacgacagat tccgaaacgt
   117661 atgcgtttat caatgagggc aacagcgatg cgcgggtgat ccctgtcggc ccgctgcata
   117721 tcacctccta tgaaccgggt cacttccgct tgtttgtgga tggcgagcaa attgtcgatg
   117781 ctgattaccg cctgttttat gtccatcgcg gcatggagaa actggcagaa acgcggatgg
   117841 gctacaacga agtgaccttc ttatcggacc gcgtgtgtgg gatttgcggt tttgcccaca
   117901 gtgtggccta taccaactcg gttgaaaatg cactggggat tgaggtgccg caacgagcac
   117961 atactattcg ctcgattctg ctggaagtcg agcggctaca cagtcatttg ctcaaccttg
   118021 gcctctcctg ccattttgtt ggttttgata ccggctttat gcaatttttc cgcgtgcggg
   118081 aaaagtcgat gacgatggcg gaattgctga ccgggtcgcg taaaacctac ggtctgaatc
   118141 tgattggtgg tgttcgccgc gatattctca aagagcaacg tctgcaaacg ctgaaactgg
   118201 tgcgcgagat gcgcgccgac gtgtcggagc tggtagagat gctgcttgct acgccgaata
   118261 tggaacaacg cactcagggc attggcattc tcgaccgaca aatcgcccgt gattatagcc
   118321 ctgtagggcc gctgatccgc gcagtggttt tgcccgtgat ttgcgctttg atcaccccta
   118381 cgccgactac ggcaatattc caaaaacact gtttaccttt accggcggcg atgttttctc
   118441 ccgcgtgatg gtccgtgtca aagagacgtt tgattcgctg gcaatgctgg aatttgccct
   118501 cgacaacatg ccggataccc cactgctgac cgaaggcttt agctataaac ctcacgcatt
   118561 cgcgctgggc tttgttgaag cgccacgcgg tgaagacgtg cactggagca tgctcggtga
   118621 taaccaaaaa ttgttccgct ggcgctgtcg tgccgccacc tacgccaact ggccggtgtt
   118681 gcgttacatg ctgcgcggca ataccgtttc tgacgcaccg ctgattatcg gtagccttga
   118741 tccctgctac tcctgtaccg accgtgtgac gctggtagat gtgcgcaagc gccagtcaaa
   118801 aaccgtgctg tataaagaga tcgaacgcta cggcattgat cgtaaccgtt cgccgctgaa
   118861 gtaaggacag aagatgctga agttactgaa aactattatg cgcgccggaa ccgcgacggt
   118921 gaaatatccc ttcgcgccac tggaggtcag cccaggcttt cgcgggaaac cggacctgat
   118981 gcccagccaa tgtattgcct gcggtgcctg cgcctgtgct tgtccggcaa atgcgctgac
   119041 tatccagacc gacgaccagc aaaattcgcg cacctggcag ctctatctgg ggcgttgtat
   119101 ttactgcgga cgttgtgaag aagtgtgccc gaccagagcc atccagctta ccaataactt
   119161 tgaactgacc gtcacccata aagccgatct ctatacccac gcgacgttcc atctacaacg
   119221 ttgcagccgt tgcgaacgcc cgtttgcccc gcaaaaaacc gtcgcactgg ctgctgaatt
   119281 gttggcacag caacaaaatg ctccacaaaa ccgcgaaatg ttatgggcgc aagcgagcgt
   119341 ctgcccggag tgcaaacaac gcgcaacgct gatcaacgac gatacagatg taccgctggt
   119401 ggctaaggag cagctatgag tccagtgctt acgcaatatg tcagccagcc catcacgctg
   119461 gacgagcaaa cgcaaaagat gaagcggcat ttgctacagg atatccgtcg ctcggcttac
   119521 gtttatcgca tcgattgcgg cggctgcaac gcctgtgaaa tcgaaatttt tgctgccatc
   119581 acccccgtat tcgacgcaga acgttttggc attaaggttg tttcatcacc gcgtcacgcc
   119641 gatattttgt tatttactgg cgcagtcacc cgggcgatgc gtatgcctgc acttcgggcg
   119701 tatgagtctg cccccgatca taaaatctgt gtttcctacg gcgcgtgcgg tgtcggcggc
   119761 ggtattttcc acgatctcta cagcgtctgg ggcggcagcg acaccattgt ccccattgat
   119821 gtttggatcc ccggctgccc gccaacaccg gccgccacca ttcacggttt cgccgtggcg
   119881 ctcggtttgc tgcaacagaa gattcacgcg gtggattatc gcgatcccac cggggtgact
   119941 atgcaaccgt tgtggccgca gatcccgcca tcacagcgta tcgccattga gcgagaagcg
   120001 cggcggctgg cgggctatcg tcaggggcga gaaatttgcg atcggctcct gcgccattta
   120061 agcgacgatc ctacaggaaa tcgggttaac acctggttgc gcgatgccga cgatccacgt
   120121 ctcaatagta tcgttcagca actctttcgc gtactccggg ggttacatga ctgaagagtg
   120181 cggggaaatt gttttctgga cgctgcgaaa aaagtttgtc gccagtagcg acgagatgcc
   120241 ggaacacagc tctcaggtaa tgtattactc gctggctatc ggtcatcacg ttggcgtgat
   120301 tgattgtctg aatgtcgcct tccgctgccc actgacggaa tacgaagatt ggcttgcact
   120361 ggtcgaagag gagcaagccc gacgtaagat gctgggggtg atgacttttg gtgagattgt
   120421 tattgacgcc agccacaccg ccctgttgac ccgggcattc gcgccactgg cagatgacgc
   120481 gacgtctgtg tggcaagcgc gtagcattca attcattcat ctgttggatg aaattgtgct
   120541 ggaaccggct atctatctga tggccagaaa aattgcgtga gaaggatttc tcattaataa
   120601 ggactgttga tggctatgtc agacgaagcg atgtttgccc cgccgcaagg aataacaatt
   120661 gaagcggtaa acggaatgct cgcggagcgg ttagcgcaga aacacggcaa ggcgtcttta
   120721 ttacgcgcct tcatcccgct gccgccgccg ttcagcccgg tacaacttat tgaactgcat
   120781 gttctcaaaa gcaacttcta ttaccgctac catgatgatg gcagcgatgt gacggcaaca
   120841 acagagtatc agggcgagat ggtcgattat tcgcgttacg ccgtccttct cggcagtagt
   120901 ggaatggcgg agctacgctt tattcgtacc cacggcagcc gttttactcc ccaggattgc
   120961 acactgttta actggctggc acgcattatc accccggttc tgcaatcatg gctcaatgat
   121021 gaagcacagc aggtggcgct gcgtttgctg gagaaagatc gcgatcatca tcgggtactg
   121081 gtggatatca ctaatgcagt gctgtcacat cttgatctcg acgatctgat cgctgacgtc
   121141 gctcgtgaga tccatcattt tttcggtctg gcttcagtca gtatggtact gggcgatcat
   121201 cgaaagaacg agaagttcag cctgtggtgc agcgatcttt ctgcctcaca ttgtgcgtgt
   121261 ctgccacaca atatgcctgg cgacagtgta ttgctgacac aaacgctaca aacccgacaa
   121321 ccgaccttga cgcaccgtgc agatgatctg tttctctggc aacgcgaccc gttattactc
   121381 ttacttgcat ctaacggctg cgaatctgcg ctccttatac cgcttacctt tggcaaccat
   121441 acaccgggtg cattgttgct ggcgcatacc tcttccactc tctttagtga ggaaaactgc
   121501 cagctactac aacacatagc cgatcgcatc gctattgccg ttggcaatgc cgatgcctgc
   121561 cgtagaatga ccgatttgca ggaaagtttg cagcaagaaa accaccagct tagcgagcag
   121621 ctcctttcga atctgggcgt cggtgacatt atctatcaaa gccaggcaat ggaagacctg
   121681 ctccagcagg tagatattgt ggcgaagagc gacagtacgg tgttgatttg cggtgaaacc
   121741 ggaaccggca aagaggtgat cgccagagcg atccatcaac ttagcccgcg acacgacaag
   121801 ccgctggtca aaatcaactg cgctgccatc cccgccagtc ttctggaaag tgagttattc
   121861 ggtcatgaca aaggggcgtt tactggtgcg attaataccc atcgtggtcg ttttgaaatt
   121921 gccgatggcg gcacgttgtt tctcgatgaa attggcgatc tgccgttaga acttcagcct
   121981 aaactgctgc gcgtattgca ggaacgggag attgagcgtc tcggcgggag tagaacgatc
   122041 ccggtaaatg tcagagtcat tgccgccacc aaccgtgatt tgtggcaaat ggttgaagat
   122101 cgccagtttc gcagcgatct cttttatcgc ctgaatgtct tcccactgga attgcctccg
   122161 ctgcgcgacc gtccggaaga tatccctctt ttagcaaagc atttcacgca aaaaatggcg
   122221 cgccatatga atcgttcaat tgacgccatc ccgaccgagg cgctacgcca gttgatgtcg
   122281 tgggactggc cgggcaacgt gcgcgagctg gaaaacgtga ttgagcgagc ggtactgctg
   122341 actcgcggta acagtctgaa tttacatctt aatgtccgac aaagccgttt actgccgacg
   122401 ctaaatgaag attcagcgct tcgcagttca atggcgtagt tgctgcaccc gacgacgcca
   122461 gagaatgacg aagaagaacg tcagcgcatt gttcaggtat tgcgagaaac caatggcatt
   122521 gttgccgggc cccgtggcgc ggcgacacga ttagggatga agcgcaccac gctgctgtca
   122581 cgaatgcagc gtctgggaat ctcggttcgc gaggtgttgt aatctgcttt tgcaggagta
   122641 tgcatgagaa acaaactctc tttcgacttg cagttgagcg ccagaaaagc ggcaatcgct
   122701 gaacggattg ccgcccataa aattgcccgc agtaaagtgt cggtcttttt aatggcgatg
   122761 tccgctggcg tgtttatggc gatgtccgct ggcgtgttta tggcgatcgg atttactttt
   122821 tacctttccg ttatcgccga tgccccgtct tcataggcat taacccatct ggtgggcggc
   122881 ctttgcttta cacttggctt tattttgctg gcggtttgcg gcaccagcct gttcacctcg
   122941 tcggtaatga cggtaatggc aaaaagtcgg ggcgttatta gttggcgaac ttggctgatt
   123001 aacgcacttc tggtggcctg cggtaatctg gcaggtattg cctgtttcag tttgttaatc
   123061 tggttttccg ggctggtgat gagtgaaaac gcgatgtggg gagtcgcgat tttacactgc
   123121 gccgagggca aaatgcatca tacatttact gaatctgtca gcctcggcat tatgtgcaat
   123181 ctgatggttt gcctggcgct gtggatgagt tattgcggac gttcgttatg cgacaaaatc
   123241 gtcgccatga ttttgtccat caccctgttt gtcgccagtg gctttgagca ctgtatcgcc
   123301 aatttgtttg tgattccgtt cgccattgcc attcgccatt tcgcccctac ctccttctgg
   123361 caactggcgc acagtagcgc agacaatttt ccggcactga cggtcagcca ttttattacc
   123421 gccaatctgc tcccggtgat gctgggtaat attatcggcg gtgcagtgct ggtgagtatt
   123481 tgttatcggg ctatttattt acgtcaggaa tcctgaaaaa tcagcccggc gaaacagttc
   123541 gtcgggctat ttatttacgt caggaatcct gaaaaatcag cccggcgaaa cagttcgtcg
   123601 ggctgaagat tattcttgcg cgatttgccc atcgggccag gcgtggatca cggctttgat
   123661 cagcgtcgcc aatggaatgg cgaagaatac gccccagaat ccccacaaac caccgaagat
   123721 caccaccgat aaaataatca ccagcggatg caggttaacc gcttcggaga acaacaccgg
   123781 tactagcagg ttgccgtcca gcgcctgaat aatcagatac accgcgaagc agctccagaa
   123841 ttcagtacct gcgccaaact ggaatagcgc cacgccaacc accggaatgg tcaccacaaa
   123901 tgcgccaatg tacggaataa gaaccgagaa gccgaccagc actgccagca gcagcgaata
   123961 gttcagcccg aagagcaaga accccagcca ggtggcgatc cccaccacga tcatctccag
   124021 cactttgccg cggatatagt tggtgatttg ttgattcatc tccttccaca cctgtcctgc
   124081 cagcccacgg ttacgcggca gcacccggcg aacggcgttc agcatctgct ctttgtcttt
   124141 cagcaggaag aagaccatca atggcaccag caccagatag acggctatgg tcagcaaacc
   124201 gaccagcgag gcgagggaaa ttttcaccac cgaatcgccc atggtcagca tccgactgcg
   124261 catattttcg gccattgcat caataatgcc cgcgtccatt aacaccggat agcggcgcgg
   124321 caacgtggcg gcaaagtcag aaagcttatt gagcatcccc ggcatatcgc ggattaagta
   124381 gatgccctgt tgccaggcga taggcagtac cacgaacgcc atcagtagca atataccgac
   124441 aaaaaccacc aatacaatcg acgtcgccca gcggcgggag cagccaatag attgcaggcg
   124501 cacggttggc cattccagca aataagccag cacaatagcc accagcaacg gagcaagcag
   124561 gccactaaag aaaaagataa tgccaaatcc ggcaactaaa ataaccagca aggcaatcgc
   124621 ttccgggtcg ctaaaacggc ggcgatacca ttgcatcaac atttcgagca taaagacctt
   124681 ccctgaacct caagagcggg attgcgatcc gcaattgtat cgaaatgtca caaaaaagac
   124741 ttcgcttttt atgacggatt caggaaactg aaaagtcatt tgagtgggct aatcttcgcc
   124801 gttacactca aaggcggcga ggtgggaacg atatttcaca gtatcggtca aatgactatc
   124861 ttcagaaata caggatagag gttatgttca ggcagttgaa aaaaaacctg gttgcaaccc
   124921 tcattgctgc tatgaccatt ggtcaggtag ccccggcgtt tgccgacagc gcagacacct
   124981 tgccggatat gggaacctcc gcaggaagca cgctttccat tggtcaggaa atgcagatgg
   125041 gcgactatta tgtccgccag ctacgcggca gcgcgccgtt aattaatgac ccgctgttaa
   125101 cgcaatatat taattcgctg gggatgcgtc tggtttcgca tgccaattcg gttaagacac
   125161 cgtttcattt ttttctgatc aacaacgacg aaattaacgc ctttgctttc tttggcggca
   125221 acgtggtgct gcactctgcc ctgttccgtt attccgataa cgaaagtcaa ctggcttcag
   125281 ttatggcgca cgaaatctcc cacgtcaccc aacgtcacct ggcgcgagcg atggaagatc
   125341 aacaacgcag cgcgccgctg acctgggtcg gcgcgttagg ttctatttta ctggcgatgg
   125401 ccagtccgca ggcggggatg gcggcgctga ccggtacact ggcgggaacg cgccagggga
   125461 tgatcagttt cacccagcaa aatgaacagg aagcggaccg cattggtatt caggtgctgc
   125521 aacgctcggg attcgatcca caggcgatgc cgaccttcct ggaaaaattg ctcgatcagg
   125581 cgcgttactc ctcgcgcccg ccggaaattt tattgactca cccgttgccg gaaagtcgtc
   125641 tggcagatgc ccgcaaccgt gctaatcaga tgcgcccgat ggtggtgcag tcgtcggaag
   125701 atttctatct ggcgaaagcg cgcacactgg ggatgtataa ttccggacgt aaccagctca
   125761 ccagtgattt gctggatgaa tgggcgaaag gaaacgttcg tcagcaacga gcggcgcaat
   125821 atggtcgtgc tttacaggcg atggaagcca ataaatacga cgaggcgcgt aaaacgctgc
   125881 aaccgttact ggcggcagaa cctggcaacg catggtatct cgatctggct accgatatcg
   125941 atcttgggca aaacaaagcc aatgaggcaa tcaatcgcct gaaaaatgcc cgcgatttac
   126001 gcaccaatcc ggtgttgcag ctcaacctgg cgaacgctta tctgcaaggc ggtcaaccac
   126061 aagaagcggc caatattctg aatcgctaca cctttaataa taaagatgac agcaacggct
   126121 gggatttgct ggcacaggcg gaagccgcgc taaataaccg cgatcaggag ctggctgcgc
   126181 gagcagaagg ttatgcgctc gccggacgac tcgatcaggc catttcgctg ttgagtagcg
   126241 ccagttcgca ggtgaaatta ggcagcctgc aacaagcgcg ttacgatgcg cgcatcgacc
   126301 agttgcgcca gctgcagaaa cgctttaagc cttataccaa gatgtaattc gcattaaagg
   126361 aagaacaatg accaaacagg taaaaatcta ccataaccca cgctgttcaa agagccggga
   126421 aacgctgaac ctgctgaaag aaaacggcgt ggagccagaa gtggtgctct accttgagac
   126481 acccgccgat gcggcaacgc tgcgcgattt gctgaaaatg ctggggatga atagcgcccg
   126541 tgaattgatg cgccagaaag aggatcttta taaagaacta aacctggctg acagttcgct
   126601 tagcgaagag gcgctgattc aggcaatggt ggataatccg aagctgatgg agcgcccgat
   126661 tatggtcgcg aatggcaaag cgcggattgg tcgtccaccg gagcaggtac tggaaatcgt
   126721 gggttaagat gtgctccatc gctagttgaa gcacattgcc ggatgcgacg cttgccacgt
   126781 cttatccggc ctacgaatac tatggattaa cttcgcgtag gtcaggcgtt cgcgccgcat
   126841 ccgacaataa acaccttatc tacaacttca gaatttcttt cacaaacgga atggtcagct
   126901 tacgttgcgc ggtaatcgac gcacgatcca actgatccaa cgtcataaat agcgtgcgca
   126961 tttctctgtc gagccgcttc agcaagaaac gccccacatc ttccggcagt tcaaaaccac
   127021 gcaaacgcgc gcgtaactgt agcgcctgca acttatcttc atcagaaagt ggctgcaatt
   127081 tgtagatctg cccccagtcg agacgcgacg cgagatccgg taatcccaga ttcaactgcc
   127141 gcggtggacg atcgccggtg atcaacaacc gtgttttgcc cgattccaga attcgattgt
   127201 agagatcgaa aatcgccatc tcccacaact catcgcctgc aatacactca atgttgtcga
   127261 tacagaccag cgacaaatgc tccataccgt cgagcacttc cggaacaaac caggtgcgtt
   127321 tatccagcgg gacatagccc accgcatcgc cacgctgcga caattccgcg caagccgcgt
   127381 gcagcagatg gctgcgcccc gcgccttcgc gtgcccagag atagatgtaa ccgctatgtt
   127441 cctgacgcag cacgttttgc agcgcggcca gtaaagagga gttatccccc ggccagaaac
   127501 ttgcaaaggt ttcgtcgtca ggaagataaa gtggcaaaga gagctgtgcc ggtgtgttca
   127561 gagatacctc aaccaggatt tcacaaaatc gcgagaagtt taccacagaa tcccatgatg
   127621 tttgaaccgg gcagcaacac tgcccggtcg gtacattatt tgtctgttat atccgcgtct
   127681 tctgcgtcca gcaccacttc ttcagggcgc agcacactga tcagtttgaa aatcaaactt
   127741 aagccgatac cgacgatggt cgccagcgcc atacctttta actccgccgc gccgatgttt
   127801 accttcgcgc cactgacgcc gatgatcaaa atcacggaag tcaggatcag gttctgtgct
   127861 ttgttgtaat ccactttcga ttcgatcaaa acacgaatac cggaagcacc aatgacacca
   127921 taaagcagca gcgaaacgcc gcccatcacc ggcaatggga tcatctggat agcggcagcc
   127981 agtttaccga cgcaggaaag caggatagcg aaaatcgccg ccccgccgat aacccaggta
   128041 ctgtaaacac gggtgatcgc catcacgcca atgttttctc cgtaagtggt atttggcgta
   128101 gagccaaaga agccggagat cacggtcgac aggccgttag caaacatcga acggtgcaga
   128161 cctggatcgc gcagcagatc ttttttgacg atattagccg ttactaccag gtgccctacg
   128221 tgttcggcaa taaccactaa cgccgctggc agaatagtca gaatggcaaa ccactcgaag
   128281 cgcggcgtat agagggttgg cagtgcaaac cagtgagcat taataatcgg cgtggtatcg
   128341 acaattccca ttgcgaaaga gagcgcgtac cccaccagca cgccaattaa aatcgggata
   128401 attgccagga aaccacgaaa cagcacggaa cctaaaaccg tgaccgccag ggtggtaata
   128461 gagatgatga tggttttgga gtttggcgtt tgcccttcag ccgggagtaa acccgccata
   128521 ccggcagcta cgcccgccag ctccagaccg atgacggcaa cgattgcgcc cattgccgca
   128581 ggtggaaaca gcacgtccaa ccagccggtc cccgctttct tcacgataaa agaaaccagg
   128641 cagaacagca cgccgcacat aataaagccg cccagcgcga cttcataccc taacggcaac
   128701 agtaacaata ccggtgaaat aaaggcaaag ctggaaccaa gataagccgg aattttccct
   128761 ttacagatga agagatacag cagcgttcca ataccgttaa ataacagtac agtcgccggg
   128821 ttaatatgaa ataagacggg caccaggacg gttgcaccaa acatggcgaa caaatgttgc
   128881 aaactaagcg ggattgtctg taaaagtggc ggtctttcac tcaccccgat agcacggcgc
   128941 gtcatagtat tatcctctgt attatgtgtt ataggcgctt tactcaaaaa aaagccgact
   129001 cttaaagtcg gctttaatta tttttattct ttatttcgta ccaaagattt tgtcaccggc
   129061 atcgccgagg cccggaataa tgtatccgtg ctcgttcagt ccctggtcaa tcgatgcagt
   129121 atacagttcg acgtccgggt gcgctttttc cagcgcagcg ataccttctg gcgcagcgac
   129181 cagcaccaga actttgatgc tgctgcagcc cgcttttttc agcaggtcga tggtcgcgat
   129241 aacggaacca ccggttgcca gcattgggtc aacgatcagc gccatacgct catcgatgtt
   129301 agaaaccagt ttctggaagt acggtaccgg ctccagcgtt tcttcattac ggtacatacc
   129361 gacaacgctg atgcgcgcgc tcggaacgtt ttccagcaca ccgtccatca taccaagacc
   129421 cgcacgcaga attggcacaa cggtaatttt cttacctttg atctggtcga tttctaccgg
   129481 gccgttccag ccttcgatag ttactttttc cgtttcgagg tcggcggtcg cttcgtaagt
   129541 cagcaggcta cccacttcgg aagcgagttc gcgaaagcgc ttggtgctga tatcttgctc
   129601 acgcatcagt cccagcttgt gtttgacgag tgggtgtttg acttccacga tcttcatact
   129661 ctttctcctt tgaggggcag ccacaaaaaa atcgacggat tatacctcct ttcttcaggg
   129721 cggcaatatt attttcgttg actttagtca aaatgataac agtttgagat aaagttattt
   129781 tatattcaaa tggttatgaa agaagattat tccatccgaa aactaacctt tgccctggca
   129841 caagtcttct ttcgccgcgc gcccggggaa aagacgtgca aaaaggttgt gtaaaacagt
   129901 ctcgcaaacg tttgctttcc ctgttagaat tgcgccgaat tttatttttc taccgcaagt
   129961 aacgcgtggg gacccaagca gtgaccgata aaacctctct tagctacaaa gatgccggtg
   130021 ttgatattga cgcgggtaat gctctggttg gaagaatcaa aggcgtagtg aagaaaacgc
   130081 gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc gctgtgtgca ttgccgcaaa
   130141 aatatcgtga acccgtgctg gtttccggca ctgacggcgt aggtaccaag ctgcgtctgg
   130201 caatggactt aaaacgtcac gacaccattg gtattgatct ggtcgccatg tgcgttaatg
   130261 acctggtggt gcaaggtgcg gaaccgctgt ttttcctcga ctattacgca accggaaaac
   130321 tggatgttga taccgcttca gcggtgatca gcggcatcgc ggaaggttgt ctgcaatcgg
   130381 gctgttctct ggtgggtggc gaaacggcag aaatgccggg gatgtatcac ggtgaagatt
   130441 acgatgtcgc gggtttctgc gtgggcgtgg tagaaaaatc agaaatcatc gacggctcta
   130501 aagtcagcga cggcgatgtg ctgattgcac tcggttccag cggtccgcac tcgaacggct
   130561 attcgctggt gcgcaaaatt cttgaagtca gcggttgtga tccgcaaacc accgaacttg
   130621 atggtaagcc attagccgat catctgctgg caccgacccg catttacgtg aagtcagtgc
   130681 tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca cctgaccggc ggcggcttct
   130741 gggaaaacat tccgcgcgta ttgccagata atacccaggc agtgattgat gaatcttcct
   130801 ggcagtggcc ggaagtgttc aactggctgc aaacggcagg taacgttgag cgccatgaaa
   130861 tgtatcgcac cttcaactgc ggcgtcggga tgattatcgc cctgcctgct ccggaagtgg
   130921 acaaagccct cgccctgcta aatgccaacg gtgaaaacgc gtggaaaatc ggtatcatca
   130981 aagcctctga ttccgaacaa cgcgtggtta tcgaataatg aatattgtgg tgcttatttc
   131041 cggcaacgga agtaatttac aggcaattat tgacgcctgt aaaaccaaca aaattaaagg
   131101 caccgtacgg gcagttttca gcaataaggc cgacgcgttc ggccttgaac gcgcccgcca
   131161 ggcgggtatt gcaacgcata cgctcatcgc cagcgcgttt gacagtcgtg aagcctatga
   131221 ccgggagttg attcatgaaa tcgacatgta cgcacccgat gtggtcgtgc tggctggttt
   131281 tatgcgcatt ctcagcccgg cgtttgtctc ccactatgcc gggcgtttgc tgaacattca
   131341 cccttctctg ctgccgaaat atcccggatt acacacccat cgtcaggcgc tggaaaatgg
   131401 cgatgaagag cacggtacat cggtgcattt cgtcaccgat gaactggacg gtggcccggt
   131461 tattttacag gcgaaagtcc cggtatttgc tggtgatacg gaagatgaca tcaccgcccg
   131521 cgtgcaaacc caggaacacg ctatttatcc actggtgatt agctggtttg ccgatggtcg
   131581 tctgaaaatg cacgaaaacg ccgcgtggct ggatggtcaa cgtctgccgc cgcagggcta
   131641 cgctgccgac gagtaatgcc cccgtagtta aagcgccagc tctgccgctg gcgtttttca
   131701 attcacctgt aaatcgcaag ctccagcagt ttttttcccc cttttctggc atagttggac
   131761 atctgccaat attgctcgcc ataatatcca ggcagtgtcc cgtgaataaa acggagtaaa
   131821 agtggtaatg ggtcaggaaa agctatacat cgaaaaagag ctcagttggt tatcgttcaa
   131881 tgaacgcgtg cttcaggaag cggcggacaa atctaacccg ctgattgaaa ggatgcgttt
   131941 cctggggatc tattccaata accttgatga gttctataaa gtccgcttcg ctgaactgaa
   132001 gcgacgcatc attattagcg aagaacaagg ctccaacgct cattcccgcc atttactggg
   132061 caaaattcag tcccgggtgc tgaaagccga tcaggaattc gacggcctct acaacgagct
   132121 attgctggag atggcgcgca accagatctt cctgattaat gaacgccagc tctccgtcaa
   132181 tcaacaaaac tggctgcgtc attattttaa gcagtatctg cgtcagcaca ttacgccgat
   132241 tttaatcaat cctgacactg acttagtgca gttcctgaaa gatgattaca cctatctggc
   132301 ggtggaaatt atccgtagcg atagcatccg ttacgcgctg ctggagatcc catcagataa
   132361 agtgccgcgc tttgtgaatt taccgccaga agcgccgcgt cgacgcaagc cgatgattct
   132421 tctggataac attctgcgtt actgccttga tgatattttc aaaggcttct ttgattatga
   132481 cgcgctgaat gcctattcaa tgaagatgac ccgcgatgcc gaatacgatt tagtgcatga
   132541 gatggaagcc agcctgatgg agttgatgtc ttccagtctc aagcagcgtt taactgctga
   132601 gccggtgcgt tttgtttatc agcgcgatat gcccaatgcg ctggttgaag tgttacgcga
   132661 aaaactgact atttcccgct acgactccat cgtccccggc ggtcgttatc ataattttaa
   132721 agactttatt aatttcccca atgtcggcaa agccaatctg gtgaacaaac cactgccgcg
   132781 tttacgccat atttggtttg ataaagccca gttccgcaat ggttttgatg ccattcgcga
   132841 acgcgatgtg ttgctctatt atccttatca cacctttgag catgtgctgg aactgctgcg
   132901 tcaggcttcg ttcgacccga gcgtactggc gattaaaatt aacatttacc gcgtggcgaa
   132961 agattcacgc atcatcgact cgatgatcca cgccgcacat aacggtaaga aagtcactgt
   133021 ggtggttgag ttacaggcgc gtttcgacga agaagccaac attcactggg cgaagcgcct
   133081 gaccgaagca ggcgtgcacg ttatcttctc tgcgccgggg ctgaaaattc acgccaaact
   133141 gttcctgatt tcacgtaaag aaaacggtga agtggtccgt tacgcacaca tcgggaccgg
   133201 gaactttaac gaaaaaaccg cgcgtcttta tactgactat tcgttgctga ccgcagatgc
   133261 gcgtatcacc aacgaagtac ggcgggtatt taactttatt gaaaacccat accgcccggt
   133321 gacatttgat tatttaatgg tgtcaccgca aaactctcgc cgtctgttat atgaaatggt
   133381 agaccgcgaa atcgccaacg cgcagcaagg gctgcccagt ggtatcaccc tgaagctaaa
   133441 taaccttgtc gataaaggcc tggttgatcg tctgtatgcg gcctccagct ccggcgtacc
   133501 ggttaatctg ctggttcgcg gaatgtgttc gctgatcccc aatctggaag gcattagcga
   133561 caacattcgt gccatcagta ttgttgaccg ttaccttgaa catgaccggg tttatatttt
   133621 tgaaaatggc ggcgataaaa aggtctacct ttcttccgcc gactggatga cgcgcaatat
   133681 tgattatcgt attgaagtgg cgacaccgct gctcgatccg cgcctgaagc agcgggtgct
   133741 ggacatcatc gacatattgt tcagcgatac ggtcaaagca cgttatatcg ataaagaact
   133801 cagtaatcgc tacgttcccc gcggcaatcg ccgcaaagta cgggcgcagt tggcgattta
   133861 cgactacatc aaatcactcg aacaacctga ataaccctat gccaatacac gataaatccc
   133921 ctcgtccgca ggagtttgct gcggtcgatc ttggttcaaa cagttttcac atggtcatag
   133981 cccgtgtggt agatggtgcc atgcagatta ttggccgcct gaaacagcgg gtgcatctgg
   134041 cggacggcct ggggccagat aatatgttga gtgaagaggc aatgacgcgc ggtttgaact
   134101 gtctgtcgct gtttgccgaa cggctacaag ggttttctcc tgccagcgtc tgtatagtcg
   134161 gcacccatac gctgcgtcag gcgctgaacg ccactgactt tctgaaacgc gcggaaaagg
   134221 tcattcccta cccgattgaa attatttccg gtaatgaaga agcccgtctg atttttatgg
   134281 gcgtggaaca tacccaaccg gaaaaaggtc gcaaactggt tattgatatt ggcggcggat
   134341 ctacggaact ggtgattggt gaaaatttcg aacctattct cgttgaaagc cgccggatgg
   134401 gttgtgtcag ctttgcccag ctttattttc ctggcggggt catcaataaa gagaattttc
   134461 agcgcgctcg catggcggca gcacaaaaac tggaaacttt aacctggcaa ttccgtattc
   134521 agggctggaa cgttgcaatg ggcgcttccg gtaccataaa agccgcccat gaagtgttaa
   134581 tggaaatggg cgagaaagac gggataatta ccccggaacg tctggaaaaa ctggtaaaag
   134641 aaattttacg tcaccgtaat ttcgcatcgc tgagtttacc gggtctttcc gaagagcgga
   134701 aaacagtctt cgttcctgga ctggcgattt tatgtggtgt gtttgatgct ttagccatcc
   134761 gtgaactgcg cctttctgac ggggcgttac gcgaaggcgt actgtatgaa atggaaggac
   134821 gtttccgtca tcaggatgtg cgtagtcgca ccgccagcag cctcgccaac cagtatcaca
   134881 tagacagcga acaggcccga cgagtgctgg ataccactat gcaaatgtac gaacagtggc
   134941 gggaacagca accgaagctg gcgcatccgc aactggaggc gctactgcga tgggccgcca
   135001 tgctgcatga ggtcgggttg aatatcaacc acagcggttt gcatcgccac tccgcttata
   135061 ttctgcaaaa cagtgacttg ccgggtttta atcaggaaca gcagctgatg atggcgacac
   135121 tggtgcgcta tcaccgtaaa gcgattaagc tcgacgatct gccgcgcttt accttgttta
   135181 agaagaaaca gttcctgcca ctgatacagc tattgcgcct tggcgtatta ctaaacaatc
   135241 aacgtcaggc aaccaccaca ccgccaacat tgacactgat tactgatgac agtcactgga
   135301 cactgcgttt cccgcatgac tggtttagtc agaatgcgct ggtactgctt gatctggaaa
   135361 aggagcaaga atactgggaa ggcgtggctg gctggcggtt gaaaattgaa gaagaaagta
   135421 ccccagaaat cgccgcttaa tgctttgcgg gccgacgaga aatgtcggcc cgcattattc
   135481 aggcactttc gcgaatgggt tcgatttcat tcagcgtatc aattaacggt tgcggcttac
   135541 caataagata accctgcata taatcgatcc ccaaagagag caccgcctcg cggatctctt
   135601 cgttttcaac gtactctgcc actacccgca ttttcttcat tcgcgccaga tggcaaatcg
   135661 acgccacaat ctgataatcc agactatttg acacaatatt gcggataaag ctgccgtcaa
   135721 ttttaagcag atcggcattc acatttttaa gccgtgcata gctggcgtag ccggtgccga
   135781 aatcatcaat cgcaatctgg cagcctaatt cctgaagatg ctgcaaggtg atttgcgcct
   135841 gcttaacatt ggtcagagca ttactttcgg tgacttcaaa aataagttgc cacgcttcaa
   135901 tctgatattt agccagcaac tgactgactt caacaggaaa acgagcctga cataccgagg
   135961 ttggagacag attaatagca aaacggtgag cgggcatctt cgctctgttt tcagccataa
   136021 attgcagcgt atgctcaatg acccacatgt cgatactcga cgataaacca aattcgtgcg
   136081 cgaccggcaa gaagctaccg gggccgatca gttcatcatt ctcacctttc atgcgcagaa
   136141 gaatttcatg gtaaacatca ccacgcatac cggtaatcgg ctgggccatc aggaaaaaat
   136201 ggttgtgttc cagcgcctgc tgtagtcgat tcatcatcgc gactttatct ttcaattcgc
   136261 gttgca
//
LOCUS       DAJTRH010000002        95195 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTRH010000002 DAJTRH010000000
VERSION     DAJTRH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(89..1339)
                     /gene="icd"
                     /locus_tag="OO339_000132"
     CDS             complement(89..1339)
                     /gene="icd"
                     /locus_tag="OO339_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5924912.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1511..2164
                     /gene="rluE"
                     /locus_tag="OO339_000133"
     CDS             1511..2164
                     /gene="rluE"
                     /locus_tag="OO339_000133"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5924913.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2174..2635
                     /gene="nudJ"
                     /locus_tag="OO339_000134"
     CDS             2174..2635
                     /gene="nudJ"
                     /locus_tag="OO339_000134"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5924914.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2689..3795
                     /gene="mnmA"
                     /locus_tag="OO339_000135"
     CDS             2689..3795
                     /gene="mnmA"
                     /locus_tag="OO339_000135"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5924915.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3831..4472
                     /gene="hflD"
                     /locus_tag="OO339_000136"
     CDS             3831..4472
                     /gene="hflD"
                     /locus_tag="OO339_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5924916.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4476..5846
                     /gene="purB"
                     /locus_tag="OO339_000137"
     CDS             4476..5846
                     /gene="purB"
                     /locus_tag="OO339_000137"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5924917.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6015..6686
                     /gene="phoP"
                     /locus_tag="OO339_000138"
     CDS             6015..6686
                     /gene="phoP"
                     /locus_tag="OO339_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5924918.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6686..8146
                     /gene="phoQ"
                     /locus_tag="OO339_000139"
     CDS             6686..8146
                     /gene="phoQ"
                     /locus_tag="OO339_000139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5924919.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8943..9224)
                     /locus_tag="OO339_000140"
     CDS             complement(8943..9224)
                     /locus_tag="OO339_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924920.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9238..10899)
                     /locus_tag="OO339_000141"
     CDS             complement(9238..10899)
                     /locus_tag="OO339_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5924921.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10883..11209)
                     /locus_tag="OO339_000142"
     CDS             complement(10883..11209)
                     /locus_tag="OO339_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5924922.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11265..11447)
                     /locus_tag="OO339_000143"
     CDS             complement(11265..11447)
                     /locus_tag="OO339_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924923.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11431..11871)
                     /locus_tag="OO339_000144"
     CDS             complement(11431..11871)
                     /locus_tag="OO339_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5924924.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAGGGFREEEERAANRNNWMYDADE"
     gene            complement(11871..12167)
                     /locus_tag="OO339_000145"
     CDS             complement(11871..12167)
                     /locus_tag="OO339_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5924925.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12164..12502)
                     /locus_tag="OO339_000146"
     CDS             complement(12164..12502)
                     /locus_tag="OO339_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5924926.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12499..13710)
                     /locus_tag="OO339_000147"
     CDS             complement(12499..13710)
                     /locus_tag="OO339_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5924927.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13712..14284)
                     /locus_tag="OO339_000148"
     CDS             complement(13712..14284)
                     /locus_tag="OO339_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5924928.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14324..15481)
                     /locus_tag="OO339_000149"
                     /pseudo
     CDS             complement(14324..15481)
                     /locus_tag="OO339_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15782..16006)
                     /locus_tag="OO339_000150"
     CDS             complement(15782..16006)
                     /locus_tag="OO339_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924929.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16148..16309)
                     /locus_tag="OO339_000151"
     CDS             complement(16148..16309)
                     /locus_tag="OO339_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5924930.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16319..16624)
                     /locus_tag="OO339_000152"
     CDS             complement(16319..16624)
                     /locus_tag="OO339_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924931.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16621..16899)
                     /locus_tag="OO339_000153"
     CDS             complement(16621..16899)
                     /locus_tag="OO339_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924932.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17188..18936)
                     /locus_tag="OO339_000154"
     CDS             complement(17188..18936)
                     /locus_tag="OO339_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5924933.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18933..19232)
                     /locus_tag="OO339_000155"
     CDS             complement(18933..19232)
                     /locus_tag="OO339_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5924934.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19238..19471)
                     /locus_tag="OO339_000156"
     CDS             complement(19238..19471)
                     /locus_tag="OO339_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924935.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19473..19693)
                     /locus_tag="OO339_000157"
                     /pseudo
     CDS             complement(19473..19693)
                     /locus_tag="OO339_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19686..19907)
                     /locus_tag="OO339_000158"
     CDS             complement(19686..19907)
                     /locus_tag="OO339_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924936.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19897..20097)
                     /locus_tag="OO339_000159"
     CDS             complement(19897..20097)
                     /locus_tag="OO339_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5924937.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20227..20406
                     /locus_tag="OO339_000160"
     CDS             20227..20406
                     /locus_tag="OO339_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924938.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20531..20860
                     /locus_tag="OO339_000161"
     CDS             20531..20860
                     /locus_tag="OO339_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5924939.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20910..21098)
                     /locus_tag="OO339_000162"
     CDS             complement(20910..21098)
                     /locus_tag="OO339_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5924940.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21473..22702)
                     /locus_tag="OO339_000163"
     CDS             complement(21473..22702)
                     /locus_tag="OO339_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5924941.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22951..24072
                     /gene="roxA"
                     /locus_tag="OO339_000164"
                     /gene_synonym="ycfD"
     CDS             22951..24072
                     /gene="roxA"
                     /locus_tag="OO339_000164"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5924942.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24121..25347)
                     /gene="pepT"
                     /locus_tag="OO339_000165"
     CDS             complement(24121..25347)
                     /gene="pepT"
                     /locus_tag="OO339_000165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5924943.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25597..26733
                     /gene="potA"
                     /locus_tag="OO339_000166"
     CDS             25597..26733
                     /gene="potA"
                     /locus_tag="OO339_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5924944.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26717..27574
                     /gene="potB"
                     /locus_tag="OO339_000167"
     CDS             26717..27574
                     /gene="potB"
                     /locus_tag="OO339_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5924945.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27571..28365
                     /gene="potC"
                     /locus_tag="OO339_000168"
     CDS             27571..28365
                     /gene="potC"
                     /locus_tag="OO339_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5924946.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28362..29408
                     /gene="potD"
                     /locus_tag="OO339_000169"
     CDS             28362..29408
                     /gene="potD"
                     /locus_tag="OO339_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5924947.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29564..30385)
                     /gene="cobB"
                     /locus_tag="OO339_000170"
     CDS             complement(29564..30385)
                     /gene="cobB"
                     /locus_tag="OO339_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5924948.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30401..31312)
                     /gene="nagK"
                     /locus_tag="OO339_000171"
     CDS             complement(30401..31312)
                     /gene="nagK"
                     /locus_tag="OO339_000171"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5924949.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31341..32585)
                     /gene="lolE"
                     /locus_tag="OO339_000172"
     CDS             complement(31341..32585)
                     /gene="lolE"
                     /locus_tag="OO339_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5924950.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32585..33286)
                     /gene="lolD"
                     /locus_tag="OO339_000173"
     CDS             complement(32585..33286)
                     /gene="lolD"
                     /locus_tag="OO339_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5924951.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33279..34478)
                     /gene="lolC"
                     /locus_tag="OO339_000174"
     CDS             complement(33279..34478)
                     /gene="lolC"
                     /locus_tag="OO339_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5924952.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34740..35813
                     /locus_tag="OO339_000175"
     CDS             34740..35813
                     /locus_tag="OO339_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5924953.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35941..39387
                     /gene="mfd"
                     /locus_tag="OO339_000176"
     CDS             35941..39387
                     /gene="mfd"
                     /locus_tag="OO339_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5924954.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39530..40492
                     /gene="ldtC"
                     /locus_tag="OO339_000177"
     CDS             39530..40492
                     /gene="ldtC"
                     /locus_tag="OO339_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5924955.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40575..40787)
                     /locus_tag="OO339_000178"
     CDS             complement(40575..40787)
                     /locus_tag="OO339_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5924956.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41072..41704
                     /gene="comR"
                     /locus_tag="OO339_000179"
     CDS             41072..41704
                     /gene="comR"
                     /locus_tag="OO339_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5924957.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41766..42305)
                     /locus_tag="OO339_000180"
     CDS             complement(41766..42305)
                     /locus_tag="OO339_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5924958.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42532..43836)
                     /gene="ndh"
                     /locus_tag="OO339_000181"
     CDS             complement(42532..43836)
                     /gene="ndh"
                     /locus_tag="OO339_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5924959.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44244..44786)
                     /locus_tag="OO339_000182"
     CDS             complement(44244..44786)
                     /locus_tag="OO339_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5924960.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44809..45834)
                     /gene="nagZ"
                     /locus_tag="OO339_000183"
     CDS             complement(44809..45834)
                     /gene="nagZ"
                     /locus_tag="OO339_000183"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5924961.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45845..46669)
                     /gene="thiK"
                     /locus_tag="OO339_000184"
     CDS             complement(45845..46669)
                     /gene="thiK"
                     /locus_tag="OO339_000184"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5924962.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46650..47291)
                     /gene="lpoB"
                     /locus_tag="OO339_000185"
     CDS             complement(46650..47291)
                     /gene="lpoB"
                     /locus_tag="OO339_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5924963.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47306..47683)
                     /locus_tag="OO339_000186"
     CDS             complement(47306..47683)
                     /locus_tag="OO339_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5924964.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47686..48045)
                     /gene="hinT"
                     /locus_tag="OO339_000187"
     CDS             complement(47686..48045)
                     /gene="hinT"
                     /locus_tag="OO339_000187"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5924965.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48378..50563
                     /gene="fhuE"
                     /locus_tag="OO339_000188"
                     /pseudo
     CDS             48378..50563
                     /gene="fhuE"
                     /locus_tag="OO339_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50623..52056)
                     /gene="ptsG"
                     /locus_tag="OO339_000189"
     CDS             complement(50623..52056)
                     /gene="ptsG"
                     /locus_tag="OO339_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5924966.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52351..53148)
                     /locus_tag="OO339_000190"
     CDS             complement(52351..53148)
                     /locus_tag="OO339_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5924967.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53159..54163)
                     /gene="holB"
                     /locus_tag="OO339_000191"
     CDS             complement(53159..54163)
                     /gene="holB"
                     /locus_tag="OO339_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5924968.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54160..54801)
                     /locus_tag="OO339_000192"
     CDS             complement(54160..54801)
                     /locus_tag="OO339_000192"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5924969.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54791..55813)
                     /gene="yceG"
                     /locus_tag="OO339_000193"
     CDS             complement(54791..55813)
                     /gene="yceG"
                     /locus_tag="OO339_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5924970.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55816..56625)
                     /gene="pabC"
                     /locus_tag="OO339_000194"
     CDS             complement(55816..56625)
                     /gene="pabC"
                     /locus_tag="OO339_000194"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5924971.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56745..57986)
                     /gene="fabF"
                     /locus_tag="OO339_000195"
     CDS             complement(56745..57986)
                     /gene="fabF"
                     /locus_tag="OO339_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5924972.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58073..58309)
                     /gene="acpP"
                     /locus_tag="OO339_000196"
     CDS             complement(58073..58309)
                     /gene="acpP"
                     /locus_tag="OO339_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5924973.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58520..59254)
                     /gene="fabG"
                     /locus_tag="OO339_000197"
     CDS             complement(58520..59254)
                     /gene="fabG"
                     /locus_tag="OO339_000197"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5924974.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59267..60196)
                     /gene="fabD"
                     /locus_tag="OO339_000198"
     CDS             complement(59267..60196)
                     /gene="fabD"
                     /locus_tag="OO339_000198"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5924975.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60212..61165)
                     /gene="fabH"
                     /locus_tag="OO339_000199"
     CDS             complement(60212..61165)
                     /gene="fabH"
                     /locus_tag="OO339_000199"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5924976.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61233..62303)
                     /gene="plsX"
                     /locus_tag="OO339_000200"
     CDS             complement(61233..62303)
                     /gene="plsX"
                     /locus_tag="OO339_000200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5924977.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62384..62557)
                     /gene="rpmF"
                     /locus_tag="OO339_000201"
     CDS             complement(62384..62557)
                     /gene="rpmF"
                     /locus_tag="OO339_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5924978.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62609..63130)
                     /gene="yceD"
                     /locus_tag="OO339_000202"
     CDS             complement(62609..63130)
                     /gene="yceD"
                     /locus_tag="OO339_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5924979.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63329..63913
                     /gene="yceF"
                     /locus_tag="OO339_000203"
     CDS             63329..63913
                     /gene="yceF"
                     /locus_tag="OO339_000203"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5924980.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64025..64984)
                     /gene="rluC"
                     /locus_tag="OO339_000204"
     CDS             complement(64025..64984)
                     /gene="rluC"
                     /locus_tag="OO339_000204"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5924981.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65557..68748
                     /gene="rne"
                     /locus_tag="OO339_000205"
     CDS             65557..68748
                     /gene="rne"
                     /locus_tag="OO339_000205"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5924982.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68945..69887)
                     /gene="flgL"
                     /locus_tag="OO339_000206"
                     /pseudo
     CDS             complement(68945..69887)
                     /gene="flgL"
                     /locus_tag="OO339_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69899..71541)
                     /gene="flgK"
                     /locus_tag="OO339_000207"
                     /pseudo
     CDS             complement(69899..71541)
                     /gene="flgK"
                     /locus_tag="OO339_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71607..72548)
                     /gene="flgJ"
                     /locus_tag="OO339_000208"
     CDS             complement(71607..72548)
                     /gene="flgJ"
                     /locus_tag="OO339_000208"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5924983.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72548..73641)
                     /gene="flgI"
                     /locus_tag="OO339_000209"
                     /pseudo
     CDS             complement(72548..73641)
                     /gene="flgI"
                     /locus_tag="OO339_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
     gene            complement(73653..74351)
                     /gene="flgH"
                     /locus_tag="OO339_000210"
     CDS             complement(73653..74351)
                     /gene="flgH"
                     /locus_tag="OO339_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5924984.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74404..75186)
                     /gene="flgG"
                     /locus_tag="OO339_000211"
     CDS             complement(74404..75186)
                     /gene="flgG"
                     /locus_tag="OO339_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5924985.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75324..76075)
                     /gene="flgF"
                     /locus_tag="OO339_000212"
                     /pseudo
     CDS             complement(75324..76075)
                     /gene="flgF"
                     /locus_tag="OO339_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76095..77234)
                     /locus_tag="OO339_000213"
     CDS             complement(76095..77234)
                     /locus_tag="OO339_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5924986.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(77259..77954)
                     /gene="flgD"
                     /locus_tag="OO339_000214"
     CDS             complement(77259..77954)
                     /gene="flgD"
                     /locus_tag="OO339_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5924987.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77966..78369)
                     /gene="flgC"
                     /locus_tag="OO339_000215"
                     /pseudo
     CDS             complement(77966..78369)
                     /gene="flgC"
                     /locus_tag="OO339_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78373..78789)
                     /gene="flgB"
                     /locus_tag="OO339_000216"
     CDS             complement(78373..78789)
                     /gene="flgB"
                     /locus_tag="OO339_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5924988.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78944..79603
                     /gene="flgA"
                     /locus_tag="OO339_000217"
     CDS             78944..79603
                     /gene="flgA"
                     /locus_tag="OO339_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5924989.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79679..79972
                     /gene="flgM"
                     /locus_tag="OO339_000218"
     CDS             79679..79972
                     /gene="flgM"
                     /locus_tag="OO339_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5924990.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79977..80393
                     /gene="flgN"
                     /locus_tag="OO339_000219"
     CDS             79977..80393
                     /gene="flgN"
                     /locus_tag="OO339_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5924991.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80433..81968)
                     /gene="murJ"
                     /locus_tag="OO339_000220"
     CDS             complement(80433..81968)
                     /gene="murJ"
                     /locus_tag="OO339_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5924992.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82168..83001)
                     /locus_tag="OO339_000221"
     CDS             complement(82168..83001)
                     /locus_tag="OO339_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5924993.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83003..83650)
                     /locus_tag="OO339_000222"
     CDS             complement(83003..83650)
                     /locus_tag="OO339_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5924994.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83661..84245)
                     /gene="rimJ"
                     /locus_tag="OO339_000223"
     CDS             complement(83661..84245)
                     /gene="rimJ"
                     /locus_tag="OO339_000223"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5924995.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84481..85689
                     /gene="mdtH"
                     /locus_tag="OO339_000224"
     CDS             84481..85689
                     /gene="mdtH"
                     /locus_tag="OO339_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5924996.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85753..86400
                     /gene="grxB"
                     /locus_tag="OO339_000225"
     CDS             85753..86400
                     /gene="grxB"
                     /locus_tag="OO339_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5924997.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86534..87094
                     /locus_tag="OO339_000226"
     CDS             86534..87094
                     /locus_tag="OO339_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5924998.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87200..88246
                     /gene="pyrC"
                     /locus_tag="OO339_000227"
     CDS             87200..88246
                     /gene="pyrC"
                     /locus_tag="OO339_000227"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5924999.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88320..88565
                     /gene="dinI"
                     /locus_tag="OO339_000228"
     CDS             88320..88565
                     /gene="dinI"
                     /locus_tag="OO339_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5925000.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88855..89109
                     /gene="bssS"
                     /locus_tag="OO339_000229"
     CDS             88855..89109
                     /gene="bssS"
                     /locus_tag="OO339_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5925001.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89224..90342
                     /gene="solA"
                     /locus_tag="OO339_000230"
     CDS             89224..90342
                     /gene="solA"
                     /locus_tag="OO339_000230"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5925002.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90390..90503
                     /locus_tag="OO339_000231"
     CDS             90390..90503
                     /locus_tag="OO339_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5925003.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90764..91330
                     /locus_tag="OO339_000232"
     CDS             90764..91330
                     /locus_tag="OO339_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5925004.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91334..91909
                     /locus_tag="OO339_000233"
     CDS             91334..91909
                     /locus_tag="OO339_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5925005.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91951..93003)
                     /locus_tag="OO339_000234"
     CDS             complement(91951..93003)
                     /locus_tag="OO339_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5925006.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93228..94148
                     /gene="lpxL"
                     /locus_tag="OO339_000235"
     CDS             93228..94148
                     /gene="lpxL"
                     /locus_tag="OO339_000235"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5925007.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94320..94517
                     /locus_tag="OO339_000236"
     CDS             94320..94517
                     /locus_tag="OO339_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5925008.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94541..>95195
                     /locus_tag="OO339_000237"
     CDS             94541..>95195
                     /locus_tag="OO339_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5925009.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     "
BASE COUNT        22892 a        25243 c        23488 g        23572 t
ORIGIN      
        1 atatgctcga tatgctatta ataatatcca cataaataaa acaacgggcg tgttatacgc
       61 ccgtttaaat atttaacaca tgtagtgatt acatattctt gatgatcgca tcaccaaact
      121 ctgaacattt cagcagttta gcgccttcca tcagacgctc gaagtcatag gttacggtct
      181 tggcgttgat cgcgccttcc atacctttaa caatcaggtc tgcggcttca gtccagccca
      241 tatggcgtaa catcatctca gcggagagaa taatagagcc agggtttact ttgtcctgac
      301 cggcgtattt cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg tcaccgatgt
      361 ttgctccagg ggcgataccg ataccgccaa cctgcgctgc cagagcgtca gaaatgtagt
      421 caccgttcag gttcatacag gcgataacat catattcagc cggacgcagc aggatctgtt
      481 gcaggaatgc atcagcaatc acgtctttaa tgacgatctc tttgccggta ttcgggtttt
      541 taactttcag ccacgggccg ccgtcgatca gttcaccgcc aaattcttca cgcgccagct
      601 ggtagcccca gtctttaaac gcgccttcgg tgaacttcat gatgttgcct ttgtgcacca
      661 gggtcacaga gtcacgatcg ttagcaattg cgtattcgat cgctgcacga accagacgtt
      721 tggtgccttc ttcagaacac ggcttaatac cgataccgca atgttccggg aagcgaattt
      781 tcttcacgcc catctcttca cgcaggaatt taatcacttt ctcggcgtcg gcagagtcag
      841 ctttccattc gatacccgca taaatgtctt ccgagttttc acggaagata accatatcgg
      901 tcagttcagg gtgtttaacc gggcttggag tgccctgata gtaacgtacc ggacgcaggc
      961 agatgtagag atccagttcc tgacgcaggg caacgttgag agaacgaata ccgccaccaa
     1021 ccggagtggt cagcgggcct ttaatggcaa cgcgatattc acgaatcaga tcaagggttt
     1081 cagcaggcag ccagacatcc tgaccataaa cctgtgtgga tttttcaccg gtgtaaattt
     1141 ccatccagga gattttacgc tcgcctttat aggctttctc gactgcagcg tcgaccactt
     1201 tcagcatggc tggggttaca tctacaccga ttccatcacc ttcaatgtaa gggataatcg
     1261 gattttcagg aacgttgagt ttgccgtttt gcagggtgat cttcttgcct tgtgccggaa
     1321 caactacttt actttccatt cacctctcct tcgagcgcta ctggtttgct cgtctgccac
     1381 cacgttgcat atgcgtttgc gtcctgcgat acggatgctt tagagcaatt ttttgttaat
     1441 gatttgtaat tgacttgtca atactaccct attgtttgcc gtcatgaaag atgcgcgtta
     1501 ttaggctata atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa cttcttttag
     1561 aaatcaccag gttaagcgat tcagctcgca acgttctacc aggcgtaaac atgaaaacca
     1621 gcccacgcgt gtgatcctct tcaataaacc ctacgatgtt cttccgcagt tcaccgatga
     1681 aaccggacgc aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg
     1741 ccttgaccgc gatagcgaag ggttgctggt gctgaccaat aacggcgcgt tgcaggcgcg
     1801 tttaacccag ccgggtaaac gcaccggaaa aatctattat gtgcaggtgg aaggtattcc
     1861 cacacaagac gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct
     1921 gcccgcaggc gcggagctgg ttgacgaacc cgcgtggtta tggccgcgga atccaccaat
     1981 tcgtgaacgc aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa
     2041 tcgccaggtg cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgcta
     2101 tgcgatggga gattactctt tggataatct tgccaatggc gaatggcgag aagtgacaga
     2161 ttaaggatgc aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc acgcagaagg
     2221 caaattttta gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg
     2281 gcatctggaa gccgatgaaa ccttagtgga agccgccgcc cgtgaactgt gggaagaaac
     2341 cggcatcagc gcgcagccgc aacactttat tcgtatgcat cagtggattg cgccagataa
     2401 aacgccgttt ttgcgtttcc tctttgccat tgagcttgag caaatatgcc cgacccagcc
     2461 tcatgacagc gatatcgact gctgccgttg ggtcagcgcc gaagaaattt tacaggcgtc
     2521 aaatcttcgt tcgccgctgg tggcggaaag tattcgttgt tatcaaagcg ggcaacgtta
     2581 tccgctggag atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc
     2641 tgcgtgatag aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc
     2701 gcaaaaaaag taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg
     2761 ttgcaacaac agggatatca ggtcgaaggc ctgtttatga agaactggga agaagacgac
     2821 ggtgaggaat attgcacagc agcagcggat ctggctgatg cccaggcggt ctgcgacaag
     2881 ctcggcattg aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa
     2941 ctgttccttg ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaag
     3001 gagatcaaat ttaaagcctt cctcgaattt gccgccgaag atttaggtgc cgattatatc
     3061 gctaccggtc attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt
     3121 ctggacagca ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg
     3181 caaagcctgt tcccggtagg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat
     3241 cttggtctgg tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt
     3301 aaattccgcg agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc
     3361 gatggcgatg aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa
     3421 ggtctgggta tcggtggcac caaagaaggt accgaagaac cgtggtatgt ggtggacaaa
     3481 gacgtcgaaa acaacattct ggttgtcgct cagggccatg aacacccgcg gctgatgtct
     3541 gtcgggttga ttgcccagca gctgcactgg gtcgatcgcg aaccattcac cggcactatg
     3601 cgttgcacgg taaaaacccg ctatcgccag accgacattc cgtgcaccgt caaggcgctg
     3661 gacgacgatc gcattaaagt gattttcgat gagccggttg ccgccgtgac gccgggccag
     3721 tctgccgtct tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg
     3781 ccgctgccgg tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga
     3841 attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac
     3901 aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta
     3961 ttgatatgaa ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg
     4021 tcgggctgga aaccctgctc ggcgtgctca atgccagcag ttgccagggc ttaaacgccg
     4081 aattaacccg ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag
     4141 gcgcgctcga cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg
     4201 atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgatcagcc
     4261 cgcttggccc gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc
     4321 aggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg
     4381 tcggcggcgg acgtctacaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac
     4441 aaattcttgc tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg
     4501 tttcccctgt cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg
     4561 aatatggttt gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg
     4621 cgcacgcagc gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg
     4681 caattgtcgc caatttcagc gaagaagatg ccgcacgcat caaaaccatc gagcgtacca
     4741 ctaaccacga cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg
     4801 aactgcacgc ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc
     4861 tctcccacgc attaatgctg aaaaccgcgc gtgatgaagt gatcctgccg tactggcgtc
     4921 aactgattga tggcattaaa gatctcgccg ttcagtaccg cgatatcccg ctgctgtctc
     4981 gtacccacgg tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct
     5041 accgtatgga gcgccagcac cgccagctta accaggtgga gatcctcggc aaaatcaacg
     5101 gcgcggtcgg taactataac gcccacatcg ccgcttaccc ggaagttgac tggcatcagt
     5161 tcagcgaaga gttcgtcacc tcgctgggta ttcaatggaa cccgtacact acccagattg
     5221 aaccgcacga ctacattgcc gaactgtttg attgcgttgc gcgcttcaac accattctga
     5281 tcgactttga ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca
     5341 ttgctggtga gattggttct tccaccatgc cgcataaagt taacccgatc gacttcgaaa
     5401 actccgaagg gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc
     5461 cggtttcccg ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg
     5521 gtatcggtta tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag
     5581 tgaaccgtga ccatctgctg gatgagctgg atcacaactg ggaagtgctg gcagaaccaa
     5641 tccagacagt tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctta
     5701 ctcgcggtaa gcgcgttgac gccgaaggca tgaagcagtt tatcgatggt ctggcgttgc
     5761 cagaagaaga gaaagcccgc ctgaaagcga tgacgccggc taactatatt ggtcgagcta
     5821 tcacgatggt tgatgagctg aaataagcct caaatcagtg ccggatggcg atgctgtccg
     5881 gcctgcttat taagattatc cactttttct tttttcactt tacctcccct ccccgctggt
     5941 ttatttaatg tttaccccca taaacacata atcgcgttac actattttaa taattaagac
     6001 agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta cgtcaccacc
     6061 ttaaagttca gattcaggat gctggtcatc aggtcgatga cgcagaagat gccaaagaag
     6121 ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc ggattgcctg
     6181 acgaggacgg tctgtcactg attcgccgct ggcgtagcaa cgatgtttca ctgccgattc
     6241 tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt gccggtgctg
     6301 atgattacgt gactaaaccg tttcatattg aagaggtgat ggcgcgaatg caggcattaa
     6361 tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct ccccccgttt caggttgatc
     6421 tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg ttcgaataca
     6481 ccattatgga aacgttgata cgcaataatg gcaaagtggt cagcaaagat tcgttaatgc
     6541 tacaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta ctgatgggac
     6601 gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt cattaccacc gttcgcggcc
     6661 agggctacct gttcgaattg cgctgatgaa aaaattactg cgtctttttt tcccgctctc
     6721 gctgcgggta cgttttctgt tggcaacggc agcggtagta ctggtgcttt cgcttgccta
     6781 cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt ttcggctgtt
     6841 acgtggcgag agcaatctgt tctataccct tgcgcagtgg gaaaacaata agttgcatgt
     6901 cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt atgatgagaa
     6961 cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaaaatga tccagcctga
     7021 ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata ccagcctctt
     7081 gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag atgatgacga
     7141 cgcggagatg acccactcgg tggcggtaaa cgtctacccg gcaacatcgc ggatgccaaa
     7201 attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct atatggtctg
     7261 gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc tgctgtgggt
     7321 cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc gcgaactgga
     7381 agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca gtctggtacg
     7441 aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc gtacgacgct
     7501 caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta cgctgcgttc
     7561 tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg agcaaatcag
     7621 ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg gcgggacatt
     7681 gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct cagcgctgaa
     7741 caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag agatcagctt
     7801 tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata atgcctgtaa
     7861 atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc tctatattgt
     7921 ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt tcgaccgtgg
     7981 tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag cccgcgaaat
     8041 caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg gtgcgcggat
     8101 ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat gtccttttac
     8161 cactacatca agtcattgat tcgatcaaac aattttcatt gaaaaagtgc caaaaatcgc
     8221 aaattgacta caccattaac tacaccgttc ggtgcactgt atgaaacaac gtggaacaaa
     8281 tagacacaag aaatatacag gcgggtaatc tttccagggg gaagcgcacc aattcatgag
     8341 gggcgttaat gtcgatatgg ggatccccat aacggggcta ccggattttt tttccggtta
     8401 acctttaatc aggctggtgg gttttacata cccttgatca ccgtaatgat gatccagatg
     8461 tgcacctttt ccaccaatgg cggaaaagct ccaaccgtaa cgatgatcgc tatggttgcc
     8521 tcgatggtct gcgtggtcat tatgaccgtt cagccagtga ccttaatcag aatttttcgc
     8581 taaaaaatga cgttggccac gtcatccgga aagcacaaaa tcacgtcgat ttttaatgaa
     8641 cggttaaacc gtctttttgt cacggtgtgt cactggttgt cactctccgt cacgattgag
     8701 gtggtggtca ttctgcccga ccgcgcatct ttgcgctatc gggaatatca aacagttacc
     8761 tcgccgactt cctcaaattg aggtttccga aaatatcaga gcgggttagc gattaagctg
     8821 acgagtaatt aaactgcgaa aatttcgtag tttctgaata tattccggcc tgctggttaa
     8881 cagtcctcag atgtgaggga tgtagccagc gcaattttgc gctttgcttt aatctcagtg
     8941 agttaccgcc gtaaccgctc cggcttcttc cagtggtacg tgattttctc cttctcccga
     9001 tacagttcaa cgcggcgatt gtaggccagc atttccagaa cgcggatccg tatgtcgcgc
     9061 atatccgcat cattaagctg gataccatca cggcgcatca cctcagcaac aacacgcgca
     9121 taattttcgg ctgtcacgct gtccggctgc gtggtctgtt cgtcagcctg ctggctgatt
     9181 ccagagacgc ggcggattaa tcgcagtatt tcggcttctg tcatgctgct gacctcatta
     9241 caccccgcta aattcttcca gtttctggcg gtggctgtcg cttatatcaa aagcaaaatc
     9301 ctcatgctct gcctggaatg taccaaacgc catcagcgcc gctacgctcg ggtctatctt
     9361 gttcggtgat tttttcttgt tcggcttgat attggcgttc gcgtcactct gcatcacgac
     9421 gttactcatc gaccaggaca acaccggatc gccacgatgc acaatcaccc tgcggttaac
     9481 aaaaacttcg aacgatttcg ccgccggact gaatctgagg taggtttgcg ggaacggctc
     9541 cacctcaaaa ccagccccct gtaattgcgt cctgagatgc gtggcgttcc acgtatcaaa
     9601 gcccaccagc ctgatattaa atttctccgc atcctgcatg atgtcatctc tgatccggtc
     9661 gtaatcaatg cagtcacctg gcgttgtgcg tatccagccc gctttagccc actggcgata
     9721 gacagcgcgg tttttattgg cggggttctg tagctggaac tccggcagat aatgacggga
     9781 aatcagcata atggttttac cgaccggaaa ggcatagcat acgctggaaa tatcgctggt
     9841 tgatgataag tccagccccg cgtaacactc ctgaccgtgt aaatcttcct ccgtgaacgt
     9901 tccggcacac tcagcccatg caccattacc catccacggg gtagtcccct gacaccagat
     9961 attaaatcgc tttgtcatca tctccaccca ctgcgacgga ataccccgcg ctttctggat
    10021 ggttgaggcc agtttttcac gatccacgga aacatcaata ttagggttcg cctttatcca
    10081 catcgccgga tcatcaacct cgttttcgtc gtccagttcg taaatcagta caaaaattga
    10141 atcgttaacc tcttcgccgt ccaggatctg gcagcaataa tcatagtgct gtttacaggc
    10201 tgaaacgacg ttactgcccg atgtggtaat ggcaaataac agcccttcgg gacgtgcgcc
    10261 catccccagc tcaagcgcgg aataaacgcc gttatcgggg tgtaggtggt attcgtccac
    10321 gatggaaaga ctggggtttg tcccctcgat ggttgccgct tttgctgcca gcggctttaa
    10381 caggctgttg cttttcgggt gtatcacctt atgcgcctga atattcaccc gcctgcgtaa
    10441 cggtcgggat aaaaggcaca tctgacgcgc attatcaaac acgatccgcg cctggtcacg
    10501 gctcaccgct gcggtgtaga tatcctgctg accgttctcc ataatcagaa accagttagc
    10561 cagaatcgcg gcggtcgtgg atttcgcatt tttgcgcggc acttcgataa aggcgctcgt
    10621 gtatttgcgc cgtccggtgg ccttaacctt aaagccgagg atgcacgcaa aggcgaactg
    10681 ctgccacggc tccagctcaa tgggtctgcc acgcatcggt ccttttacgt gcgggcacac
    10741 cctggaaaag gcaataaacc gctccacaac ctcacgatcg aacgtgtaaa gggggctttt
    10801 aaggtccgaa aagtaccgtt taacagcctg ttttaaccgt ttacaggccg gaattttgcc
    10861 cgtttttacg tcttctgcgt acttattcca ggcggtcaag ctcgtcctct tcttctgttt
    10921 ccggtggatt tttacggcgg cttatcgggt caaaaccgag caaggaggcc atttttatca
    10981 tcactctttc agcgtcggat tttgcgctta atgcggggtt tctgctctcg ccgccctgac
    11041 tgttaacaat gctgaacccg cgcgccgcaa ggtctgcaac ggctttccgg taaatggagt
    11101 agttgacgca atacagttcc agattgctcc agtcggcggg ggtcaggtct ccccgttccg
    11161 caagctgccg cgatttttcc cgccactgct tcacggcgat atcatccagg taggcggggt
    11221 cgggtttgtc ctgaaaacgc cccccacccc gtcatacagc ctcatcagcg attgcggaaa
    11281 cattccataa cctcgcggtc acggtcggtt aatcgcttcg ctgtgatgcg ttctgcgcgt
    11341 cctgacgctt tatctttatg ccctgtttcc tgtgtcttcc atgcgtcacg ctgccttata
    11401 agtccacgga taaggcggtt ttgttcccgc tcattcatca gcgtcataca tccagttatt
    11461 gcggttagca gcccgttctt cctcttcacg aaatccacct ccggcacgct tgctttttgt
    11521 tgccggatca agccatttcg ttttctggtt atgacacgcc tggcacaatg gttgatggtt
    11581 ccattcgggc cagaagagaa catcatcacc gccattaatc gggataatgt gatccaccac
    11641 cacggcgggc gtatatatcc ccttctcaag gcatcgcacg cataacgggt ttttactcag
    11701 atacatggcg cggtatttgt cccactgtct ggaatatcca cgcgcgcggc ggtgtcctcg
    11761 tctggcatcc tctgcacgcc atgccgcccg cctgtgctcc tcacacttgc cggatttaac
    11821 gcgcctgtta cagcctggtt ctgtgcatcg tcttaatggt tgccacggca tcagtacccc
    11881 cccacatcac ggtaagccgt ccagagtgcg cctatcgtca tgggtacgcg tgtttttgcg
    11941 ttatccgcga caatctggcg attttcatac aggtgagcga taaacatcat gcagccaatc
    12001 tttatggctg gcgtgaacgc cagcccgtca tcaaagcgcc tgcctatgtg tatctggcac
    12061 gcctcaagcg atgcggcaat gtatccgctg attaactggt cttcatcgtc gccatcgatg
    12121 cggcaatgga gtttcacttc ttccagggtg ataagttctt ctgtcatttt tccgcgccct
    12181 cacgacaaag aatttcaagg cgtgtcctgg cggcatccgg cagcggctgc ccgatgatat
    12241 tcagtacacg ccccgccagc ggccccgtac tgacctttat ccggctggtg gcgttgatgt
    12301 ccttccggta ccgtatccag atccttacgg taccaatcgc cagctctgcg cccgatgaaa
    12361 tggcctcctt gctgctgacc atgttcacgc ttgcccagag tgtgtgaccg tcctcccacg
    12421 tttcgagtat ttcgccggtt atatccctgg tctgtttcag ggtctgaatc gttatcctgt
    12481 cacgcagtcg ccctatgttc attccggttt ttctccctcg ctgattttta cttcctgttt
    12541 ccatgcctgg ctgaactcat caccaccatc acgcggtgac agcccttcac gttcgcgggc
    12601 ttcgttcgga cacataacgt cggattttat gccgcgctca taggtggcaa agcgttcgcc
    12661 aggtgtggcc cgtaacaggt ccgcgctgtc aaactccacc tgataccgga taccaggtac
    12721 aggcgaggcc accagcaagg cgttttttat ctgctgctca aagttcgcca gccacgggcg
    12781 catcgtcatg gtgagaaatg cgcggcttgc ctcgctgaag ttgctgtagg tgctgttgct
    12841 gtattcctgc aaaaaaatcg gcgagacgtt gaacattcgg gcgatgtctt caatggtgaa
    12901 gcgacgggag gccagccatt cggcatcctg attactcatg cccagctgct gatagctcat
    12961 ccccccttca aggatgggcg tttttccggc gtttctggcc cctttgtagc gttccagtgc
    13021 ggctaatgcc tgtttgccct tcacgccgtc cagccattcg cctgacgtga taacccctgc
    13081 cgccatcatg ccatcacgca tcacgctcgc gccgtggcgt tgttgggcca gcccaagccc
    13141 cagcgattcg cggcagatgg ttacaggtga gcgccccata aaaccgtcat ccgtggagta
    13201 acgaaggtgg agaacctccc acggtaaata gttgcggatg tttccggtgt aggcgtcagt
    13261 gatgcagtag cgccagttat gatcacctgt ctgctccacg ttcaccgact gcggcgggta
    13321 aggatgtaaa gccgccggaa aaccatcacg cccccactga ataaccgcat aagcattacc
    13381 acttaacagg cagtagcgga tcatcattcg cttaaactgg tagggtgtct gccacgcgtt
    13441 cgggcgctcg ttgaggatgt gatcgaccgg atgagaatca agccattcgc gggcctcctt
    13501 ccccttctca ttgcgtacca ggtacaggta acacggcatg gtagccaccg cgtcagagat
    13561 gaccgtgacg gcgttcatga cggcggggag tgattccgct gtcccagatg atacgtactc
    13621 acccgccccc gtgttcgatg tgccggccag cgccataaat tcatcaagcg tcatgctgcg
    13681 ctgctctttt tttcgtctga aaggccacat atcacacccc cgctaaatcc gcccaccagc
    13741 ggcgattatc cgcacgcggc attttttcgg ggtgctgctc atacagggaa cggcgggcca
    13801 gctccacgcc ggaatcgggg taagccggta ccgatgtaac ggtaatttcg tacagttccg
    13861 ccaccagcac ggtgcgcacg catggatctg ttgtggtatc ccatacatcc ttacgggagc
    13921 gaaagccaaa gctcatcccc gagatgtccc cacgcttaac cagttcgata acgtcgcgcc
    13981 ctgtactggt atccggtggg gtcagttcaa agcgtaaccc tgtttcgtcc tcttccagtt
    14041 tcagcgtgcc ggaacgggtg cgccccagta acatgctgtg gtcatgctca tacaggccgc
    14101 gaacgtcatt acccgccgca agccattcag taaacgcccc ccgctggaat ttttcgtaaa
    14161 actcacccca taacagttct gaaagcttat cccagcgaac aacgtagccc gtcagcgtac
    14221 cggctccgct ggtggtgatt tccgatgacc ggatttccat actcttcata attttttcac
    14281 ccataaaaca ctaaaggggc tttttagccc cttctgtatg ctgttaatcg tcgtcttccg
    14341 gcagtgccag aatcttgatc gcgttcgaat ccaccacgcc accgcctaaa tatttttgcg
    14401 tgaaaatttt gatgaatcct ggttctgtca ggttgtccgg tctggtgcga acacctgttt
    14461 cgtgatcaac aatgtagtaa ccgcgtttga agtcacccag ggcaataacg ttatcaggca
    14521 taaactccag atattcgacc ggaaggccca gcaacgtatc aggatcaccc gcctgtaaac
    14581 ggtcgcgcca gatataatca ccgttcgcgt tcttcacctt ctgaagtttt gccgccgtcg
    14641 tggagttaac cactcagacc gcgtttttgc ggtatttttt acgtaatgca aatttcaggt
    14701 cgatcagcgg gtccgcagat gtccacgcca gagattcgga aggtttaatt acctgcaacg
    14761 taccaaaatc acgctctttg tcgttcttct ctgcacgggg tacggataaa aaaccttttg
    14821 cttttttgtc accgtcgccc acaaccagat cgctttcttc ggtttccgtg aaggtgtcgc
    14881 caatctcacc cgtcagccat gaaaggatgt ccacatcgga aaaatccacg atttcctgtg
    14941 tggtgcgcgg gtacgcatag accggataca gcttaatgct cacctcgtta atctgcgggg
    15001 tgctggtctg ttcgcgtgcc ttaccctctt caccgtggtt aacggtcgca ccgccagcgg
    15061 aaacaagctg cttaaactcg ttgctgctga ttttcttcac ggtacagatg cgacgcatgg
    15121 tggattcatc cgccagcatt cgcatgattt cggtgttcag ttcagggata acggtataac
    15181 caccatcagc gggaacgcct gtacttaatg cgcgggtttc accggtcaga atgtagttac
    15241 gtagttctgc ggggtcagtg gtctggctgc ttttacctgg cttgctgcgc tcttcgtctg
    15301 caatggcttc aaggcgggaa atgtctttat cgagggattc agctttagcg cgtaattcgt
    15361 caaattttgc gccctcagca tcgttaagac tgcggttttc tttttcagcg ttctccagca
    15421 tgtcgcgcat ctgattttta atggcggttt tctgctggcg taattcgatt attctctgca
    15481 taaaaaaagt cctggggtta agtaaggaac tccaggacgc ggcaaacact caaccgtttt
    15541 tcataaggaa atcagtaatc gcaccgatcg ttttcccgcc tggtaatgaa tattggcgag
    15601 cacattaaca ggcgggaaag tggccccagc gtcctggcac cacgggcgag aataatcatg
    15661 attcagttcg ggtaaaatat gccgatccgc tcagtgaaca acgtggaaca accacgaaca
    15721 aataatttac aaaaaataac aaaaagccgg attgctccgg ctgtatgtgc tgtatgggtg
    15781 ctcagtctct gatccctttc agaaattcta tcaatgcatc tgtctgttca ggatttactg
    15841 ccagcatttc accggatagc gcgcaacgaa caaaaccatg atgatccttt tcaatcagcg
    15901 cccccgtttc cagaaatgcg cggtaatcag tgatattcat cgtctccatg ttgtcagcat
    15961 ggtatttatc acgctgttta agtatctcat caaatttcat cggcattgcg ttttcctctg
    16021 ttgtgtctgt ttgtttcaga tagtaactat gccggaccgt gacgaaaagc cagtagtgct
    16081 ccataccgtt tcaaatgacg caaaaaaagc cggattgctc cggctgcgtg gtgtggttct
    16141 gatattccta ctctgctaat ttgctcagcc ccatgtctgc gtaagtgcga tttactgcat
    16201 ttctcaggtc tgcgtagttc tccgttggcg gctccggtgt gcccttgctg ctctgtttta
    16261 ccagcgggtc gcccaccagc tgcccgtaag ctgcaatttg tgctgtcata ttgccacctc
    16321 agtgaagcga tccggcattg ttctttttca tctcttccat cattttttca gtgagcatta
    16381 cgcactgaaa ccccatgttt ttgatgtcta cgggtttcat gcctttgtca gtggatatca
    16441 caggcagaat atcctgatac acatcgcggt cagggtaact aagcacacaa agctcccatg
    16501 atgccacggg tgaggatcct tcgtctgttt caacccacca gcctggtgta gctgcgctta
    16561 tttgcagaat cgtgatccgt ccgttgtgct tgtaaaattt ttcaccaaat tttggttcgc
    16621 tcatgattcc acctctccgg ttttaacgtt gattgttgtt acctgttccg cttcggcaat
    16681 ctcccgctct gtcagcgtgg caaaatttgc cgccgtcgtg gtcatgaatg cgcttatcag
    16741 ttcgggatgt gctttcgcgt atccttcccc cgcgtggcgg tctatcgttc tgattgccac
    16801 ctttaaagcg tgctctgtca tgtctaacgc gcgatatttc ggttctgttc tgtctctgcg
    16861 ttttttgaat gatttattaa atttccctga agtgtgcata tttattttta ccccctcgtt
    16921 taaaaagttt tgagttgtgc ctccccttgt ctaccttatc taccttagtg gccctcatgc
    16981 cagtaatggc gctgctttca gcggggtaga gtgcttttat ccactatcta ccccgtgtct
    17041 acctccctgt ctgattcagg taaaatcagg tagagagggt agatagtggg tagacagtaa
    17101 aaaaaggcta tctacctaac ttaatgcact gaattaaatg tatttttctt tactcaggta
    17161 gacagggtag acagcaatta caaaaaatta taaaaacgcg tcgcactcgt ctgttgttat
    17221 tgcgttagtc tgcgttactc ccttaacttt ccgcgtaata tattcatgtc cgtaaacttt
    17281 cgcggctggc ttcatagcct tgccaaagtc atttacgttt agcggtttgc tcctgcctgc
    17341 gtacgccata aacgccagat agacgcggta aaggctgttt ctggtcgtgt acttcactga
    17401 atcgccaccg ccgcccatca tcaggccgcg tgcttcctcc agaaaattca ggaactggca
    17461 aaactcaata accggatccg tctgttgctt tattgccaga gcttcatcac cgtcacgctg
    17521 ttccagtagt aaagcccgtg ctttctcaga gtcggtaaag ttcgccagca atcggcggat
    17581 aatgacaggg atttcagccg caatcttttc cggtagctcc ctgtcttttt cggcctcact
    17641 aacgatattg tcgaaacgga aaatcacgcg acgacgtgcc acacctccgg cccgttcggt
    17701 gaatatcatc gggttattgt tggtcgccag caccaccgcc ctgattacag ccgtgaaacg
    17761 cttttcgtat ttcgggttaa tttccacggg gtcaccgccc gtgattttct tgatgcccgt
    17821 tccttcgcct gtatatttcg gctggtcagc caggacgata agacgactcc cgacaacctg
    17881 cgcacgtcca ccagcatcat caagcgatgt catttcagcg cttaccgtgt tctgtttccc
    17941 tgccagaagg ctggctatgt gtgtgaatgt acttttaccg ctcccgccgt ctccggtggc
    18001 ctcaataaac atctgccagt cgtaccggtt cgccataatc atgtacagcg cggcacatat
    18061 acgcatcatc ttgcgctggt cttttccggc tgcgtgctca agccatttat gaaagtttag
    18121 cgcgttatcg cggatgttct cccctggtgc tggtggcgtg tactcaatgc cgttgtgcgt
    18181 ggtgatccag ttctccggcg tgtgcgggga aaattccccc gttttcaggt caagcgcacc
    18241 attggcgaac ggcagcaaat cgccggacgg ctcgcccatt ggttcggcaa taacttttaa
    18301 cgcttccacg gcgttattga ttacgcgctt gctgaaagtg gccctgtgct ctgaatagat
    18361 caccaccatt tcgcggctaa gttccattgt gctgaccgga caccataccc cgccgcgcca
    18421 tacgtgaacg atttcacttt cctgatgcac acaaacgcca tcaaagcgct cggcaagcag
    18481 ctgcgcgcgc tcactgtctg ccatttgtgc cagttgcgta tttttctgct ccggcatggt
    18541 aagccctgcg gcaatatttt cacgctcagc attcagatag cgccgccagt tttcacactt
    18601 ctggacgtgc attccttcgg ggtaaaaatt tgcgtcctga atatctgccg ccgccagctt
    18661 ctgaccaatc tttttggtct ccaccaaatc cagttctccg gcctggtaca gccttacgcg
    18721 tttttttcca tccggaacaa ttttcagtgc atcaagttcg gcaagctgat ttggcccaag
    18781 ccacacaggc ggcacattat cgccggatgc ggggccatcc tgctcctgcc attgtttcgc
    18841 gtgcgaccag gcatcactac ccgcaaagat aattacctct gtgtctttgt gttttattcc
    18901 gcgtggctgt ttttttacgt tcggtgccag tttcattttt tacccctgaa tacgttaagc
    18961 atctttttta tttcctgaat attggcgcgt gctttctccc tgctggttgg tttactgcgg
    19021 ggcgctgcct gtaccagcgt gaaatcacgc cggaattgat aaacaggcat cacgcaatca
    19081 tattcgtaac cttcacggcg gtaggtgatg cgccgttctg ccacgccttt aatcgttacc
    19141 atgccgccgt actggtcgcg gtaaatatca ccgcgcgtaa atttagggtg agtgttgcca
    19201 ctggcagtta agccagaata tttaagtttc attattttta ttctccggtg tgttgttctt
    19261 tatatctgtc gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg
    19321 ttattaatgc cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt
    19381 caagccatat gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc
    19441 ggtctattgt ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa
    19501 ataacctctg tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg
    19561 atttatattc tctcgtggaa agtaagaaaa aagccgctct aataagttct ggcattgacg
    19621 aaagcgcatc agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac
    19681 gatcatcacg cataaccccc cccattttca caatcggcaa caagaatatt tttagcgtca
    19741 ttcagtgacc gcgttgcgat gtatcggata cattccaggg cgaactgtgt ccggtctttc
    19801 tgttcttctc cctgcgcaag ctctgccgtg cattcaatat caataagcgc gtgcatcagc
    19861 gtagtgagtg cggcggctgc tgcgttcggt gtggttttat tgtgcatagg tttccccctg
    19921 acgaatacgg gcggcgaata cagcaacaca accggacggg caacggctac gcgcttcgcg
    19981 ttccgtccaa gcggttacgt ggatgatttg ggattctccg gcactcagtg ccagaaaacg
    20041 ccacacaaag gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg caaccataac
    20101 ggctcctagt ttacgttgtt ggttagaagc cccgttactg ctcctacagt gcggggcttt
    20161 gctttttgtg ttgattgtgt gtaatgtgtc actacacata aatacattac ctgaggtgta
    20221 attaacgtgt caacacacaa aaacgaaagg cgtggtaatc ctccgttcca gttccgttta
    20281 gatcctgact tacgggaaaa gatggaaaag gcacaacaac aggacggcga tgattctctt
    20341 gctgcctgga taaagcgaat aatccgcaaa gaattacagc aacgcggcat agaacccaaa
    20401 ggataaaaac tatcagcgcc gtggtgtgag gtactacggc gcattgcttt acaggcgcac
    20461 acaatgacca acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag acgacgcaga
    20521 aagaaagagc atgaatcaga acgattcgcg ccgtgttctt ttgcccttga gaagtacctt
    20581 aaagaatact catcaaagga gagaacttcg cagttatggc aacgcactga accagactga
    20641 ttgcattgcc caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac
    20701 acacaaacgg cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt
    20761 tttatgcctg aaaaacccca attttgtggt tttccagttt caccagggcg aacgaatccc
    20821 cgcccacgtt ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga
    20881 tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag
    20941 gccagaacct cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg
    21001 aattgtccgg ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg
    21061 cattcttttt cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta
    21121 aagagttatt tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga
    21181 gttggcaaat gttgaggccg tatggtttac agaaagagga ataatcagga taaaatcctt
    21241 taaattcatg ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta
    21301 aagagtgatt cgctagtgta taaaaaatca gtaacacatt aaaaatcagc tacttataaa
    21361 tctgtacact ttttcacctc ttgttcgtag ttgttccgca ttgttgctca ttgttgcacg
    21421 ttgtatctgt tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc
    21481 ttctggctac tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct
    21541 ccgtccacat atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc
    21601 gctgcattcc tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta
    21661 aatcgttcag catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat
    21721 ttagcctcct gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc
    21781 ccagcaataa gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg
    21841 cttccggtat gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc
    21901 agagcatttc ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc
    21961 atccgaacac aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt
    22021 ccagtgcctg caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg
    22081 gttttttccc aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc
    22141 gcctccggca gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag
    22201 gcgttcgctt tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca
    22261 gcggcatagc accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat
    22321 aatccacgcg gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg
    22381 ttaccggctt taacgcttcc tgtacggtgt aattaagctc atgacgtgga tttttcccct
    22441 ctgccagcca tgcgcgacac tgggcggctt tttccctggc tgatttcagg ctcagatcag
    22501 gataatttcc cagcttaatg cgttccggtg gtgcccccct tcccgttccg gccctgtaag
    22561 tgaaatacca ggttaaaagg ccactggttg aatgcctgac gctcaggttt ccaccgtcat
    22621 taagaaaggc tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca
    22681 gtttgttaag tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta
    22741 actacaccga tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca
    22801 caaatctgtt tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa
    22861 acactaattg ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg
    22921 gttgcagata ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc
    22981 gattttcttg aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat
    23041 tttattgacc cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac
    23101 agtcgactgg tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc
    23161 tacgatcatc tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat
    23221 gagccgaccg ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat
    23281 ctgatgattt ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac
    23341 gtgtttatca ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg
    23401 aaacagcact gcccacatcc ggatctgtta caggtcgatc cgttcgaagc catcatcgat
    23461 gaagagctgg agcctggtga tattctttat attccgccag gattcccgca tgaaggctac
    23521 gcgctggaaa atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta
    23581 attagcggat ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat
    23641 ccggatgttc cacctcgtgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg
    23701 cgtgagatga tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag
    23761 tttatatccc agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca
    23821 gatgaaatct acgatgcgct gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc
    23881 gtattgcgca ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt
    23941 ccggcactgg atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg
    24001 ctggaagatc cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc
    24061 ttcgaagggt aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc
    24121 ttacttccgt tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat
    24181 accttccaga atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt
    24241 cgggcaaggt aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt
    24301 cagttccggt tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg
    24361 atgctcaacc actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta
    24421 gcaatcagga tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg
    24481 cgcttcaaac tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac
    24541 ggtgcctttc atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct
    24601 ttcatccgcc ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac
    24661 tcctttcgcc gtgcccggat gaacattgtt accgacaatt tttatattga ccgatgcggc
    24721 gttgaagttt tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg
    24781 ggcatcgaag gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt
    24841 aaaggcgacg cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt
    24901 catgatttct gcaatacctg ctttgtcatc agcacctaac aaggttttac catcggtggt
    24961 aatcaacgtc tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc
    25021 atcgccgata cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac
    25081 atttttgccg ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat
    25141 atcgccaggg acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac
    25201 attgataagc cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc
    25261 ttccgtgctg ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt
    25321 caaaaatcgc tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt
    25381 aagaagcaag aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat
    25441 ttatcagcat taccttaatg aatataaagc taagccagta attcacaaca aagattggcg
    25501 attcaagcgg tttgccgtag aattaccgcc ctcattagga gtcaccaagg tggttaacca
    25561 caaaccccgc atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat
    25621 aaacaaccga gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat
    25681 ggtaaagagg tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg
    25741 cttggccctt ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact
    25801 gttgattccg gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac
    25861 cgctatgtga acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa
    25921 aatgtggcct ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg
    25981 atggaagccc tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc
    26041 tctggtggtc aacagcaacg cgtcgccatt gctcgagcgg tggttaacaa gcctcgtctg
    26101 ttgttgctgg atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac
    26161 gagctgaaag cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag
    26221 gaagaagcac tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa
    26281 gacggcacgc cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt
    26341 ggcgaaatta atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc
    26401 gccaacgttg aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa
    26461 aaactgcatg ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac
    26521 cacgctgaag ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag
    26581 tcggttgttg aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac
    26641 gatcctgact ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg
    26701 gaggtcgtac tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat
    26761 tgtcggttgg cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt
    26821 tttgacccgc gacgacgcca gtttcgtcaa aatggtcttt acactggata actacacgcg
    26881 tctgctcgat ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc
    26941 caccctcgcc tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca
    27001 caaggtgcgt ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat
    27061 tcgtatctac gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct
    27121 ctggctgggc gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat
    27181 cggtctggtt tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa
    27241 actggataag ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt
    27301 tatccgcatc attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat
    27361 gctgccagcg atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct
    27421 gatcggtaac gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc
    27481 caccagcatt acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc
    27541 tcgtttgctg aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt
    27601 atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt
    27661 aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg
    27721 atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg
    27781 gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt
    27841 cgcggtaagc cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg
    27901 atggcaattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg
    27961 ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc
    28021 ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt
    28081 accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg
    28141 ttaagcttta ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt
    28201 tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt
    28261 aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt
    28321 attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc
    28381 acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca
    28441 acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca
    28501 ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg
    28561 ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg
    28621 tcgataaaat gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact
    28681 tcagcaatct cgatccagac atgctcaaca agccttttga cccgaataac gactactcca
    28741 ttccgtatat ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat
    28801 ctgtcaccag ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg
    28861 acgatgcccg tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca
    28921 ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg
    28981 cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca
    29041 tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt
    29101 ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa
    29161 acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg
    29221 ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag
    29281 aagtggcaaa cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc
    29341 agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag
    29401 gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta gcaaggatgt
    29461 ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct
    29521 tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca
    29581 acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt
    29641 cattaccaac ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg
    29701 cttcgtgtac aaacccagcc gccggataga catgcccgga agtaccaatg gcaataaaga
    29761 catccgccat cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc
    29821 aaacgacgtg cgggcgcaag ggtgccggga actggcaaca atggcattta tcttctggcg
    29881 taacgtctcc ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt
    29941 ccccatgcat atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct
    30001 gcgttaccag caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg
    30061 cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt
    30121 gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt
    30181 cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg
    30241 cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga
    30301 aaaaaatacg ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta
    30361 accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga
    30421 aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgtggaacac
    30481 gagctacagg taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga
    30541 aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc
    30601 ccagagaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc
    30661 cttgatcata aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat
    30721 acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc
    30781 cgcagcggcg taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca
    30841 tatggccaaa ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca
    30901 gcccgccgcc aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt
    30961 catcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc
    31021 gatcaagacg tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag
    31081 cggcatacag cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac
    31141 ctttacagcc aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg
    31201 cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat
    31261 caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc
    31321 cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg
    31381 gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg
    31441 tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata aatatcgctg
    31501 gagaggaact gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt
    31561 gaaacaacta cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca
    31621 taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg
    31681 atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc
    31741 acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga
    31801 tacatatagc cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca
    31861 cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct
    31921 gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa
    31981 ctgtgatcga gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt
    32041 ggctgcatca gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc
    32101 tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct
    32161 ttaaaattgc gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc
    32221 tgttgtgggt taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga
    32281 ccggtgaaat tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc
    32341 agtgcttcct gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc
    32401 accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg
    32461 atcaataccg ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc
    32521 atgccgccgc gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc
    32581 gccattactc cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca
    32641 gttggcggct catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc
    32701 cctgcaagcg attcaactcc ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga
    32761 ggttaccggt aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa
    32821 tcgctacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct
    32881 ccagccccac cgcttttaac atctccagtg cacagctgtt gatttcagcg ggctttttct
    32941 tgccaatcag cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt
    33001 gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca
    33061 gtttgctcat cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc
    33121 ctaacaggtg cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt
    33181 caccttcgcc cacgctgaaa ctgacattga gcagcacatc ggtttgcaca ctgccttcct
    33241 gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca
    33301 gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc
    33361 atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg
    33421 ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg
    33481 ccaagcgccg ctccgaggat tgcaccgata atcccggcgc tagccccttg caccataaag
    33541 accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc
    33601 ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc
    33661 aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct
    33721 ttacggtcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag
    33781 tcaactctca gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa
    33841 cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg
    33901 ttggcggcga aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc
    33961 gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac
    34021 tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt
    34081 ttcacattga ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc
    34141 accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg
    34201 acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag
    34261 ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg
    34321 cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg
    34381 ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccactgcacg cccacgcatg
    34441 taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag
    34501 caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg
    34561 tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccata ttttatcatc
    34621 aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac
    34681 ctcgggtcat aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga
    34741 tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga
    34801 tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg
    34861 aagtcctgag caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg
    34921 tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa
    34981 attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc
    35041 agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg
    35101 cctccaatgc cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca
    35161 gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta
    35221 gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg
    35281 ttcccacgcc gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg
    35341 gcgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc
    35401 tgctgatggc ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc
    35461 tgctgttgtc gctggtatcg attgtggtga tcatgaagct gttttatcag tacgagcaac
    35521 gattcggtat gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct
    35581 acaccaccca tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg
    35641 cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt
    35701 tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg
    35761 atctgttgtt ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca
    35821 ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg
    35881 cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt
    35941 atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc
    36001 gagttaaccg gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt
    36061 ccggtggtac tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc
    36121 cagttcaccg atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt
    36181 ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg
    36241 atgcagcgtg gcatactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac
    36301 agttttctcc acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca
    36361 ttacgaaccc aactggacag cgccggttat cgccatgttg accaggtgat ggagcacggc
    36421 gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat
    36481 cgtcttgatt tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag
    36541 cgcacgctgg aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat
    36601 aaagcggcaa ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat
    36661 ccggaacata tttaccagca agtgagtaaa ggcacattac ctgctgggat cgagtactgg
    36721 cagccattgt tcttcagcga accactgcca ccgctgttca gttatttccc tgccaatacc
    36781 ttgctcgtga atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg
    36841 gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc
    36901 tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa
    36961 actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac
    37021 ctggccgttc aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact
    37081 ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa
    37141 ctgctcgcac gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac
    37201 cgtgggcgtt atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggcgcgtaat
    37261 ctggcgctga tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat
    37321 tctcgccgca ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt
    37381 cagccggtgg tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa
    37441 gctggcggca ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat
    37501 gttccggtgt cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc
    37561 ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa
    37621 gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc
    37681 ttcgcgttta aacacgatcg tgagcagtat cagttattct gcgacagctt cccgtttgaa
    37741 accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg tcagccgctg
    37801 gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc
    37861 gccgctttcc tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt
    37921 cttgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc
    37981 gaaatgatct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg
    38041 gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt
    38101 aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag
    38161 cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca
    38221 cgtacgctga atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc
    38281 gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag
    38341 gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa
    38401 aacattcaga aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc
    38461 ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa
    38521 cgtttcaacg tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc
    38581 aacaccatta tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc
    38641 ggtcgcgtcg gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa
    38701 gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg
    38761 gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc
    38821 gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa
    38881 aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag
    38941 caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg
    39001 aacacgcgtc tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa
    39061 gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg
    39121 ctggatattg cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt
    39181 aatgagaaag gcggggtgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg
    39241 attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa
    39301 tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt
    39361 gaactggaag agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg
    39421 caacatttac aaattctttg cactttcctg cactatccga cactgtcacc atccataatt
    39481 caggcttatc tgtttattac aataacctta tatttattat gtatttttgg tgatgatcaa
    39541 aacgcgtttt tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct
    39601 accggcaaaa gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa
    39661 caaatttcat gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa
    39721 cgtcggcttt ctcgctctgt tacaagctaa ccccggcgtt gatccttacg taccgcgcgc
    39781 gggcagcgtg ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat
    39841 tgtgatcaac attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac
    39901 cgtgtatcca ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac
    39961 caccgtttca gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg
    40021 ttataaagca cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat
    40081 gggccatcat gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa
    40141 cgccgatttc ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga
    40201 tatcaaaaca ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc
    40261 gataaaagtc tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc
    40321 agagaagatt gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc
    40381 atttaaagat gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc
    40441 cgggatgccg gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga
    40501 taaaaaaaat ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta
    40561 atgagggtta atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt
    40621 acagaagtaa tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct
    40681 tccagcgatc ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct
    40741 tctggcgttg actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc
    40801 gccgcagcga tgaggttttt acgtttttca taatagtggc cttatgcaga tgaatgacgt
    40861 cgcaagaaat tacttaacga tcgataacta aatgatagat gtgatctgga tcacatacaa
    40921 gatatttttt ataacaatca ttaattaatt taaaaatctt taaatttcag tcaaatagaa
    40981 agcaattatt tttatgggat ctggactggt gaacggaggt gattattttt ataataacct
    41041 ttcattcaca gtcaccaggt acaacggcat catggcaact gactcaacac aatgtgtaaa
    41101 aaaaagccgt ggccgcccaa aagtgttcga cagggatgcc gcgcttgata aggccatgaa
    41161 attgttctgg caacacggtt atgaagcgac ttctcttgcg gacctcgtcg aagcaaccgg
    41221 agccaaagcg cccacgctat acgcggaatt taccaacaaa gaggggttat ttcgtgccgt
    41281 tctcgaccgc tatatcgatc gttttgccgc taagcatgaa gcacagctgt tttgtgaaga
    41341 gaaaagcgtg gagtctgcgc tggctgacta ttttgctgcc atcgccaact gctttaccag
    41401 caaagacacc ccggcaggct gcttcatgat caacaactgc accaccctct ccccagattc
    41461 aggagatata gccaatacgt tgaaatcacg ccatgcgatg caagagcgca ctttgcagca
    41521 gtttttatgt caacgacaag cgcgcgggga aatcccgacc cactgtgacg tgacacatct
    41581 ggcagaattc cttaattgta ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc
    41641 gctggaaaaa ctgatgcaga ttgccggaac gactttgcgt ttatggcccg aactggtgaa
    41701 ataagtcgca aaaaaggccc ctcagcctga gcgaatgagg ggccaaattg cagagtacag
    41761 ccttgttata ctttgttatt caaaatcagt tgcccgttac tatctagcgg gatctgcgta
    41821 cccggatcgc ggtccatgcg gattttgccc tgctgatcgc caatcttata ggtcacatca
    41881 taaccgagca ttttttctga cttgtcatac accgttttac aacgctgttg cgtagtcgtg
    41941 taagtatcgc tttcctggag agagccctgg atctggttac cggcatatcc accacccagc
    42001 gcccccacaa cagtggcgac atctttaccg cgaccaccac caaactgatg ccctatcacg
    42061 ccaccagcaa cagcgccgag caccgacccg gtaatgcgat tttcatcctg caccggtcga
    42121 cgatgggtca ctgtgacgtt gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt
    42181 ggggttgcag aaacaacctg agcgtattgc gggccccgtt caaacacgtt cagactggcc
    42241 actgccgcta cgcccaacgc agctgcgaca ccaatcccga tacccgccaa cattgattta
    42301 ttcacgggaa cacctccttc tttgcagtta tgccaatcat gcaagcgaag gagaaaagca
    42361 gctatcagac agaagatgaa aagcgcggga atgactggaa atacttaagg attcggagga
    42421 gtctgaagga atgcctgatg cgacgcttaa cgcgtcttat caggcctacc gctcgtacca
    42481 tttgtccagg gcggataagg cgtttatacc acatccgcca gtgtacgtcg attaatgcaa
    42541 cttcaaacgc ggacggataa cgcggttaat actccccacc agcatcatta atccggtttt
    42601 aaagtaacca tgcagcgcaa tctgatgcat tcggtatagc gagatatata caaagcgcgc
    42661 aattcgtcct tcaatcatca ttgagccgcg cgtcaggtta cccatcaggc taccaacggt
    42721 ggagaagttc gacagcgata ccagcgaacc atgatcttta tactgataat ttttcagcgg
    42781 cttaccgttc atctgcgcca gaatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc
    42841 ctgagcacgc ggcggaacaa agcccccttc cggacgcggg catgacgcgc agtcgccaat
    42901 agcgtaaatg tctggatcgc gagtggtttg cagcgtcggt tccaccacca gctggttgat
    42961 acggttagtt tcaagaccac cgatatcttt caggaagtct ggcgctttga tcccggctgc
    43021 ccataccatc agatcagcct caatatattc gccatcttta gtgtgcaggc cgccttcatc
    43081 agcactggtg accatggttt gcgtcagcac gcgaacgcca agtttcgtta gctcgttgtg
    43141 ggccgcagca gagatacgtg gcggtaatgc aggcaaaata cgttctcccg cttctaccag
    43201 cgttacgttc agggcttcgt tggtcaggcc tttgtaaccg tagctgtgca gttgcttgat
    43261 cgcgttgtgc aattcagcgg agagttctac acccgtcgcg ccgccgccga caatcgcaat
    43321 gttcactttg ccatttgcgc ccaggttggc agagtatttc aggaacagat tcagcatctc
    43381 ctggtggaag cgacgcgcct ggtgcgggtt atcgaggaaa atgcagttct ctttgacgcc
    43441 tggcgtattg aaatcgttag aggtgctacc cagcgccatg accagggtgt cataggcgat
    43501 tttacgttcc ggaaccaaca gttcaccttt ctcgtcgcgt agttctgcaa tagtgattgt
    43561 tttcgcttca cgatcaatat caatgacgga acccaactgg aactggaaac catgattgcg
    43621 cgcatgggcc agatagctca acgcatcgac gccttcatca agcgagccag tcgccacttc
    43681 gtgcagcagt ggtttccaca ggtggctgtg gttacgatcg accagcgtaa ttttggcttt
    43741 tttcttacgt cccagcttat gccccagctg tgttgccatt tccagcccac cagcaccgcc
    43801 gccgacaatc acaatctttt tcaatggcgt agtcaacgtg ccccccttaa atttattaac
    43861 caattgttaa ttaatggtta ttaacatagc tcttaattaa caacaggtta cagcaatgaa
    43921 aatgttcttc gctattgaga ataacatgaa tggtgcattg gtcataccaa aattgatgtt
    43981 aatcaagttt tgttgcttaa aagataggca actttaccgg gggagatcaa aaaatagagt
    44041 ttattgttta ttatttgagc aaattcggaa gggtaatata gggtgaagcg tatatcgagg
    44101 gcgtttggtc gtcctgataa gatgcgcagc atcgcatcag gcattgatgc tcgactgtcg
    44161 gatgcggcgt ggacgcctta tccgacttac agaattcgga gcgcatcagg taccggcaag
    44221 gttacctgat gcgacgaggg catttacccg agggttttga acgctttaat gcgctgtaaa
    44281 tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa
    44341 tgatgcaact cttcagatgt ccgctggctg ttaagtgctt catcattacg cgacaaaata
    44401 accagacaac gatcgcgatt cttctcgcgg aagttggtca cacacttagt ggcaatatcg
    44461 gcatactctt ccgggcgatc aatcttgcct tccatgttct cataagggaa caaattaggg
    44521 ttgaagatca cctggcggat atcgcaaaga aaaccaatcc gttccgccca gtatccgccc
    44581 aggccaacgc cgcaaattag cggacgctcg tcaacgttca gttgcaacat tttgtccact
    44641 tctttaagca gatgctgcat atcatgtttc ggatgccgcg tactgtagct tatcaagcgt
    44701 acatccgggt caataaactg caattgtaag actttctcgt ggttacccgg actgttagag
    44761 tcaaaaccgt gtaaatagat aatcatcgct tcctcacata agccagggtt agtgacctac
    44821 tttctcttcc tgccagcgtt catgtaactg attcagacgg gtgctgatcg ctttccagcg
    44881 agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg
    44941 ttctgccttg atcggtgaca gattatctaa cacgctgacg gcccctttac gattattgca
    45001 gaccaggatc atatcgcaac ccgcatccag tgatgcctgc ccgcgttcgg cataactgcc
    45061 cataatcgcg gcaccttcca tcgataaatc gtcagagaaa atcacgccgt caaaccccag
    45121 ttcctgacgc aaaacggttt tcagccagta gggagaaccg ctcgccggac gcggatcaac
    45181 atcactgtag atcacatgcg caggcataat ggcgtcgagt ttattttcgc ggattaagga
    45241 actgaagacc gacatatctt tagcgcgaat ctccgcttgt ggacgtggat cgcacggtgt
    45301 ttctttgtgt gagtctgccg ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt
    45361 cattccggct tcatgcatac catcaataaa ccggctggca attgccaggg ctttttgtgg
    45421 atcggcatga taagaacgct cgccaatcgc cgcgctgata tgcccgacat ccagcactgg
    45481 cgcaaagctg atatcaatat ccatagcgat catttcgctg gccatcagcc aacccgcctc
    45541 ttgcgccagt ttgccgccct cttccattcc caacagcgca gcaaaggatt gtgctgccgg
    45601 taagcgggta aaaccttcgc gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac
    45661 caccagatga ttgcgcgatg ctgcgcggat ctggcgcacc agttcacgta actgggcagg
    45721 atcatgatag ttacgcgtaa agagaatcag ccctcccacc agcggatgcg ccagtatttc
    45781 acgctcttcc gcgtccagtt cgtaaccttc gacatccaac attactggac ccacactgct
    45841 ctccttattg ttttattaat agctgccgcc aggtgtcatc ggccagcctg ataaattgtt
    45901 gatcgccggt ttgtcgccag cggtactcaa accaccctcc tttgagcatc agcagccagg
    45961 gaaaccatcg cctgacctga cgccataatt gcgccggata aatcttcgcg cgagtggcat
    46021 agtcattgac caattgccgg tgctgttcag tattttccac ccacaccgcc gccagttcca
    46081 gcgcgatatc accatctcca gcatactccc agtcgatgag ttttaaccct gatgcgctat
    46141 gcactaaatt tccggcgtgg acatccatat gcaatggact taagcgtaaa ggtcgtggtt
    46201 cccgcgcttt gcgcagacgt tttaacattc gcagccaacc cacagtccgc cgcgccggat
    46261 cgctttgctg ccagtacagt tccagtaagg gcaacagcgt tattcgccag ccaaaacgtg
    46321 gttgttgatg tagataatac agcaagcctg ccagttcgtt ggtatccggc aaatacgttt
    46381 ttaccgcacc gggcagatag tcgactacca tccagtcacg gagatataaa tgcggcttcg
    46441 gtgcaatgct tgcgggtagt tgtgataaag cccgatactg gcgcaagaac gcggactgcg
    46501 gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt tcaatgagaa
    46561 aactaccgcc actaagccca ctattaaacg tcgtgacggg atgaaactgc gggaaaaagc
    46621 gcgacagcaa ttcgtcgcgc gtgatgggat tattgctgcg aaacggcacc tttacctgac
    46681 cagataattt cgcccgtctg caccagcatc agctgcattt gtagggtcgg agcgttaacg
    46741 ttgccagagg cgcaggagta cagcacgtaa tgagcgccga cattgcgggc aatgcctatg
    46801 gctttactac gggtgcctaa actgtcctgc ggcgacaaac ctaactgttg cttagccatc
    46861 gacagttgct gggcggaaac cagggtaaat ttcccgttat tagccagcgc atttcgcagc
    46921 gtttcggtcg cttctgcggc attcagcgaa ccgttagtac ggttgttaac gctatcaacc
    46981 agcaggacgc tacccgcagt caccccgtca gccccaagca tcttactgac catcggctgc
    47041 attgcgccat tccagtcata atggcgaata tgcggcgcag gcggtgccgt tcgatcttcg
    47101 tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg agggcactgt ggggacaggc
    47161 tgttgtggct cggctggttg ttccggcgct ggtttcactt cttctaccgg tgcaggttca
    47221 cgttgcccca cacacccggc gagaaacatc gccagcgcgg taatcaaggc gtagcgactc
    47281 atttttgtca tcaagattca cccctttaca aatatagata aagtctgact ttgtgcgccc
    47341 ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg tgcgggaatg gtaacgctgc
    47401 gtggcctttc cagaggatgc atctccagcc ctctggcgtc ataccagtaa aaacgataat
    47461 gaacggtaac gggttcttgc ctttcgttat aaatcgttga ggatgctgaa ggttgaatat
    47521 cagacgtcga aaggacgggc ttttctgcac tgatgcccgc agccaataaa gatgactcca
    47581 tcaccagcga ttgctcatca ttcaccggaa tttccggatg tgaacgacag cccaccagca
    47641 gtaacaacac cagagacacc agcccaaagc atccttttct catcgttaca gacctttatg
    47701 cgccagcatt ggtcccagcg gacggccacc caacaagtgc atatggatgt ggtaaacctc
    47761 ttgtccgcca tggcggttgg tattcatgat cagacgatag ccatcttcgg caataccttc
    47821 ttgctcagca atttttgccg ctacggtgat catgcgtccc agcgcctgct catgctcagc
    47881 tgacacgtcg ttcacagtcg gaatgagaat attcggaatg atcagaatat gcgttggcgc
    47941 ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc tggtagacga tatcggaggg
    48001 gatctcacga cgaataattt tgctgaatat agtttcttct gccacgacat tttccttttt
    48061 caaatagcca tgcgttgcgc catgctacgc cgcgccacac tgcgagtata gagtatggtc
    48121 gagttaaacc tgctctttca atcttaactc acgatttctt aagcaaaaaa tcgttttgct
    48181 gccgtatata tcgccattat tcccatttct gcctgcgaat gcgctgaaaa gacattctct
    48241 gtttggttta cacgacaaat atgaatgcgt atatttctca tttgcatttg cagcaaaatt
    48301 attcgcacac taaatataac cgctcatcac cctcctccgg catgagcctg acgacaacat
    48361 aaaccaagag atttcaaatg ctttcaacac aatttaacag ggataatcaa tatcaagtca
    48421 tcaccaaacc gtcactactt gccggttgca tagcactggc actattacct tctgccgctt
    48481 ttgctgcacc agccactgaa gaaacggtga ttgttgaggg ttcagccaca gctccagatg
    48541 atggcgaaaa tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc
    48601 aacatgatat tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt
    48661 tacaaacgct gggcgaagtg atggaaaaca cgctggggat cagcaaaagt caggcggatt
    48721 ccgatcgtgc tctttattat tcccgcggat tccagatcga taactatatg gttgatggta
    48781 tccccaccta ttttgaatcg cgctggaatc tgggcgacgc actttctgat atggcactgt
    48841 ttgaacgcgt agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat
    48901 ctgcggcaat taatatggtt cgaaaacacg cgacctgtcg tgaatttaaa ggcgatgtct
    48961 cggcggaata cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca
    49021 ccgaagacgg taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc
    49081 tggaccgcta caatagtgaa aagaccttct tctcgggcat tgtcgatgct gatttaggcg
    49141 atcttacgac gctttcagcc ggttacgaat atcagcgcat tgatgttaat agcccaacct
    49201 ggggcggttt accgcgctgg aatactgatg gcagcagcaa cagttacgat cgcgcacgca
    49261 gtaccgcacc tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga
    49321 agcagcggtt tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat
    49381 ttgacagcaa aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg
    49441 ggccatacag taattatgga cctggctttg atcatgtcgg cggcaccggt tggaacagtg
    49501 gcaaacgtaa agttgatgcg ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc
    49561 gtcagcacaa tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tcagttcatg
    49621 ggccaacatc ttcccggatg aaattggcag tttctacaac tttaatggca atttcccaca
    49681 agccgactgg tcatcacaga gcctggcgca ggacgatacc acacatatga aatcgttata
    49741 tgctgccact cgtgtcaccc ttgccgatcc gctgcatctg atcctcggcg cacgttatac
    49801 caactggcgg gttgatacgc tgacttacag catggagaaa aaccacacca cgccttacgc
    49861 tggtctggtg tttgacatca atgacaactg gtcgacctac gccagctata cctctatttt
    49921 ccagccgcaa aatgatcgtg acagttcagg caaatatctg gctccaatca ccggtaacaa
    49981 ctacgagctg ggtctgaaat cggactggat gaatagccgt ctgaccacca cgttagccat
    50041 cttccgtatt gagcaggata atgtcgctca gtccaccggt acacctatcc ccggcagcaa
    50101 cggcgaaacc gcctataaag cggtggatgg gacagtcagt aaaggggtgg aatttgaact
    50161 caacggcgca attaccgaca actggcagct gacatttggc gcaacgcgct atcttgcaga
    50221 ggataacgaa ggaaacgccg ttaatcctaa tctgccacgc accacggtta aaatgttcac
    50281 cagctatcgg ttgcctgtca tgccagagtt gacagtcggc ggtggtgtta actggcaaaa
    50341 tcgcgtgtat accgacaccg tgacaccgta tggcaccttc cgcgccgagc aaggtagcta
    50401 cgcgctggtg gatctcttca cccgctacca ggtgacgaaa aacttctcgt tacaggggaa
    50461 cgtcaataac ctgttcgaca aaacctacga taccaacatg gaaggttcta tcgtctacgg
    50521 cgcaccgcgt aatttcagca ttaccggcac gtatcaattc tgaataacac ctgtaaaaaa
    50581 ggcagccatc tggctgcctt agtctcccca acgtcttacg gattagtggt tacggatgta
    50641 ctcatccatc tcggttttca ggttatcgga tttagtaccg aaaatagcct gaacaccaga
    50701 accagcaacc actacgcccg ctgcgcccag tttcttcagg ccagcctgat ccactttaga
    50761 cacatcagca acgctgacgc gcagacgggt aatacatgcg tcgaggttag taatgttttc
    50821 tttaccacca aatgcagcaa ccagagccgg tgccatttcg ctggtacctg ttgcttttgc
    50881 atcttcagtc gcgtcttcac gacccggcgt tttcagatcc agtgctttaa tcagcacgcg
    50941 gaagatggtg tagtaaacaa tcgcataacc gataccgacg atcggtaaca gccacagttt
    51001 gctgctgtta ccagacagaa cgatgaagtc gatcagaccg tgcgagaacg acgtaccgtc
    51061 acgcatcccc agaagaatac agattgggaa tgccaggcct gccagaatcg cgtggatgat
    51121 gtacaggatc ggcgcaacga acatgaagaa gaactcgatc ggctcggtga taccggtcag
    51181 gaacgaggtc agcgccgcgg agatcataat accgcccact ttcgcgcggt tttctggttt
    51241 agcagagtgc cagatagcaa ttgcggcagc tggcagaccg tacattttga acaggaagcc
    51301 accagacagt ttacccgcag tcgggtcacc cgccatataa cgcggaatgt cgccgtggaa
    51361 aacctgacct gctgcgttgg tgtattcacc aatctgcatc tggaaaggta cgttccagat
    51421 gtggtgcaga ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc caaacgcaac
    51481 taccgggttc tggtaagcag cccactgaga gaaggtctgg attgcagaac caatcggcgg
    51541 ccaaatgaag gacagcacaa cgccagtaaa gatggcagcc aggccagaaa tgatcggcac
    51601 aaagcgttta ccggcaaaga agccaagata ctcaggcagc ttaatacggt agaaacggtt
    51661 aaacatgtac gctgcaatcg caccggagat aatccctccg agtacgccag tatccgccaa
    51721 gtgtttagag gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa ccacggccat
    51781 ggttttaacc atgatgccat aggcaacaac tgcggccagc gcggatacgc catcgttatt
    51841 ggtaaagccg agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa agacggaacc
    51901 gcctgcttct gccataacat gcgatacaac ggcgggcagc cagctgaaat tcgcggaacc
    51961 gacgcccagc agaatacctg cgataggtag tacggatacc ggcagcatca gcgatttacc
    52021 gaccttttgc aggttagcaa atgcattctt aaacataatt gagagtgctc ctgagtatgg
    52081 gtgctttttt tacgttctca cgcgtggcaa ggggggagag cctcgccgtg tacagggcat
    52141 ctaagcgccc tttatttatt acacagagta aaataattca gtgccaattt gtttgacggc
    52201 tatcacgttt caacttcaga tcattggaga agttgcacat tttgacaaaa catatgtcac
    52261 aatatttcaa acgcggttat tcaccgccca attttcggcg gtgaacttta ctcgttttag
    52321 ccattaatta cgagctttaa aaaaactcat tcaacggatg gattgaaggc gggaagcgtc
    52381 gatgtgaaac agacgggcga agttatcggt ggttacctgc gccagttctt caacggcaac
    52441 acctttcaac acagccatgt attctgcaac gtcacgaacc atcgcaggtt gattctcttt
    52501 tcctcgatgc ggtaccggcg caaggtaagg tgagtcagtt tccaccagta atcgatccag
    52561 ggggacataa cgcgcagcat cgcgcagttg ctccgcatta cggaaggtca caatgccgga
    52621 aaaggagatg taaaatccga gatccagtaa tttacccgcc gtttctctgt cctctgtaaa
    52681 acagtgtagt acgccaccgc aatccgtcac tttttcttcg cgcagaattg ccagcgtatc
    52741 ggcacgggcg tcacgggtat ggacgataac cggcttgttc agttcacgac caatctggat
    52801 atgatggatg aaggactctt gctgacgtac tttagtttcc ggtgtgtaat aataatccag
    52861 cccggtttca cccagcgcta caacaccctc ttctgccgcc agacggcgta aatcttctac
    52921 atcgtaggga tcattctggt tgagtggatg cacgccacaa gaaaatacga cgttgtcacg
    52981 ttcgcctacc agatcccgca tatgtaaata acccggtaat gttgtggcga ctgccagaca
    53041 aaatttcaca tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct tatgcaaaga
    53101 ttcataatcc agaccatcga gatggcagtg tgagtcgact aaaaacatga tgtctctctt
    53161 aaagatgagg aaccggtaac acaacgcccg gttgcaggta atgctcaata cgcagtaaaa
    53221 gatcggtgat gagaagctcg cggttgatgc ctgtgacaga cattaactgt tcacgaatgt
    53281 ggcaaacatc ccccagtata gcctgcaggc acgagggaga aagatggttt gccagttcgg
    53341 cgaccaggcc cggcacatca acattggtca cctgcgcagc gccatgatgg cgttttagcg
    53401 catccatcag caacgttgcc agccagtgta aacgcgccgg agcttgttca tgattaaggg
    53461 ccgccagcag cgaataccag tcgcccgatg gcacgctata tgccaacgcc tgacacaatg
    53521 tttcacgagc ctgccagtta tctccctgaa acaacgccag tgccgcgcca ggcgaaccgg
    53581 cgcttaagcg caatgcggca agtaatgcat cctgtgacat tgtcacttcg cgtgaaagcc
    53641 aagtcacggc gtactgttct ggaggcggcg caaggtaatg taaccgacaa cgactacgta
    53701 atgttgccag taaacgttca ggctcgcggg tagccaggaa aaaccaggtt tctgctggtg
    53761 gctcttcaag cgttttcagc aatgcgttag ccgcggcgtc ggtcagtaag gcagcatcgg
    53821 ttacccagac aactttcgca ccacctaggc gtgcgtgctc attcagcttt tcggtgacct
    53881 cacgtaccgc atcaatgccc agcgtatttt tccctttttc gggagccagg gtgtagtaat
    53941 cgggatgcgt gccagcctgc atcaactgac atccacgaca gtgaccgcaa cttttgtggc
    54001 cctgcggttg ttggcagagt aaataacggc tcagggcgta gattaaagca tcatcgccca
    54061 tgcccggtaa cgcctgaatg agtagcgcat ggtgacctct tccggcctga tagctggcta
    54121 ccagtttttc gaaatcaggt cgtaaccatg gataccatct catgcgtcca actccttcac
    54181 ccagtgggtc acggtagtgc ggattgcatc catcacggcc tccagcggct gggtggcatc
    54241 aatggtatga atacttttat cttgtgcagc cagttccaga tagcgggcgc gggtgcgatt
    54301 aaagaaatcg aaagactctt gctcaatacg atccagctcg ccgcgcgcac gcgcgcgttt
    54361 taagccaact tccggggtaa catcgagata gagcgttaag tctgggcgaa aatccccgag
    54421 aacagcatca cgcagtgttg ccagcatatg ttggtcaata ccacgtcctc cgccctgata
    54481 cgcctgagtg gaaagatcgt ggcgatcgcc aatcacccag gtgccgttag ccagcgcagg
    54541 tttgatgacc gtttctacca gttgaacgcg cgcggcataa aacatcagaa cttcggcttt
    54601 atcggtaatg acttcatcgc ctaccgattt gatatccagc accaggcttc ttaacttttc
    54661 ggcaagttgc gtaccgccag gttcccgagt gaaaaccatg tcgcggatac ccagttgctc
    54721 gagcgtctca accaccacat tacgcgcggt agttttgcct gcgccttcca gcccctcaat
    54781 gacgatatac ttactgcgca tttttttcct taagcacttt cagataatcc tgcacagact
    54841 tgttatgact ggcaagattg gtattaaacg tgtgaccacc tttaccatcg gccacaaaat
    54901 agagatacgg cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc cccggcgtag
    54961 ctatcgcacc cggcggcaaa ccggtaatgg tataggtgtt atacgctgtc ggcgtttcca
    55021 ggtctgcacg agaaagtttg ccattataac gctctcccat cccgtaaatc acggttgggt
    55081 cggtctgcag gcgcatacca atgcgtaaac ggttgataaa taccgaggca acctgatcgc
    55141 gttcactggc aacggcggtt tctttttcga taattgatgc catcgtcacc agctgatttt
    55201 tatctttata aggcagaccg tccgcacgcc cttcccaggc gctatcgacc gctttcacca
    55261 ttttcttgtg aactcgcttg agtaacgcga catcggtggt attggcggta tacatcaagg
    55321 tgtctggcca gaaccaacct tcaatccact ccgggttttc cagttcaagt gcctgcgcta
    55381 cggtggcgta cttatcgtca ctcagcgtgt gcttgatata cggagcctca cgcaattgct
    55441 tgaggtagtc gctcagacgc atcccttcta ccagtcgcag cgggaactgt gcttctttac
    55501 cgctttccaa caatttcagc atctcgcgca cggtcatctg cggtgtaaag cggtaagtcc
    55561 cggctttaaa gtgagaaaga tccggttcga tacgcagcag ccattgaaaa acccgtgggc
    55621 gattgatgat cttatcagca taaagctgtt caccgagcgc cagacgtccg gtccctggct
    55681 tcagggtaaa tatcgtctct tctttgataa gcaatttgct gtcggcaaga tggcgaacct
    55741 tccagacgcc cacaccagcg gcgataccca gtaccaccag caataacaag ataatcaata
    55801 acactttttt catgactaat tcgggcgctc acaaagtggg gctaaatatt catataacgt
    55861 tgctgacgaa aaggtgacat cgccacaggc acgtacgggc atcactggca ttaacgcatt
    55921 acaaataacc atctcatctg cctgcaacgc ctcctccagc gaggcttgca cttcgacaag
    55981 ctgataagag gattgtgcca gtaaacggat acagaattgt cgcataatgc cgttaacacc
    56041 tgcctgatcc agtcgcggcg tataaactac attgcccttc cgccagaaca aattagccgc
    56101 acagcattcc gtaacccacc cttcgctgtc aaggaccagc gcctcatcag cgtttgtctg
    56161 ctcaagatga gagcgaatca atacttgctc aagccgatta agatgtttaa tacctgcaag
    56221 atgaggattg cgccccagcc gcaccgggct tagcgccaac gtcatccctt cgttacgcaa
    56281 acggtcgtaa tgtgcaggat aagccgtaac ggagagaatc cgcgttgctg gtccgctgtt
    56341 caatgtgctg taccctcgcc cgccactacc gcgactgatc acgactttca gcacaccatt
    56401 ttgctgttct gctgccagcg ttttcatctc ttgttcaagc tgaggccaga agtcacagga
    56461 aatcatcaac cgctgacaag catcctgtag tcgcaggata tgcgccgata acaaactgac
    56521 tttaccgtcg ataactctgg cggtggtaaa acaaccatca ccaaactgcg ttgcccgatc
    56581 gcttactgcc agcgattcct gcttataacc gttaattaag aacataccgg ctccttatca
    56641 gagaattgtt agtgtggcag catgttcact acggaacaag tcggaataaa agctaagaaa
    56701 aaaggcccgc aagcggacct tttatatggg tggaaaatga caacttagat ctttttaaag
    56761 atcaaagaac cattagtgcc accgaagccg aaggagttac acagcgtgta ttccattccg
    56821 ctgacctgac gtgcttcatg cggtacgaaa tccagatcgc aaccttcatc cgggttatcc
    56881 aggttgatgg ttggcggaac agcctgatcg cgcagcgcca ggatggagta gatagattct
    56941 actgcacccg ccgcacctaa caggtgaccg gtcatagatt tcgtggagct taccaacaca
    57001 cggcttgcag cttcaccgaa gatggttttc accgcctgcg cttcagcttt atcgccagcc
    57061 ggcgtagaag taccgtgcgc gttaacgtag ccaatctgac tcgcttcaat gcctgcatca
    57121 cgcagagcat ttgccattgc cagagctgcg cctgcgccat tttctggcgg tgacgtcata
    57181 tgataagcat cgctgctcat accaaagccg acgagttcag cgtaaatttt cgcaccgcgt
    57241 tttttcgcgt gttcgtactc ttcaagtacc agcataccgg caccatcgcc cagtacgaag
    57301 ccatcacgct ctttatccca cgggcggctc gccgcttgcg ggttatcatt gcgggtagat
    57361 aatgcacgtg ccgcgccaaa accaccaacg cccagcggcg tactggcttt ctctgcgcca
    57421 cctgcaacca tcacgtcagc atcgccatac gcgataatac gcgcagcatg gccaatgttg
    57481 tgcacgccgg aagtacaggc ggtcgcgata gagatgcttg ggccacgcag gccatacatg
    57541 atagtcagat gacctgccac catgttcaca atcgttgacg gaacgaagaa tgggctgatc
    57601 ttacgtggac caccgttcat cagagatgtg tggttttctt cgatcagtcc gaggccgcca
    57661 atcccggagc caattgcggc accaatgcgg gttgcgttct cttccgttat ttcaaggcca
    57721 gaatcctgca tggcctgaac gccagcgaca attccatatt gaatgaaggc atccatcttg
    57781 cgctgttctt tgcgcgagat aatgtcctca cagttaaaat cctttactaa gccagcaaat
    57841 ttcgttgcat aggcgctagt atcgaaatgg tcgattaggc tgatgccact ctgaccggca
    57901 agcagagctt tccaggtaga ctctacggta ttgccgacag gagacaacat gcccagtccg
    57961 gtcacaacta cacgacgctt agacacgttt gtcctccaag gagggaaaaa tgattctagt
    58021 gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc acttacgcct
    58081 ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca gcttcttcgt
    58141 ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg gtgtcaagag
    58201 aatccgcgcc caggtcttca acgaaagaag cattgttggt aacttcttcc tgcttaacgc
    58261 ccagctgttc gccgataatt ttcttaacgc gttcttcgat agtgctcata ctcttaaatt
    58321 tcctatcaaa actcgctttc gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc
    58381 aactaaatcc cggcaggtct taccacgatt ttacgttatt ttgaggcctt accccccaat
    58441 aacgcaaatc attttgcact aattattact cataaccacg cagactgcgc gcaacatgag
    58501 cattttgtgc aaatcgcggt cagaccatgt acatcccgcc gttcacatgc aaagtttcac
    58561 ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg
    58621 cgccgccgag gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca
    58681 gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac aacgtttaca gtaataccgc
    58741 gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat caagcccgct ttcgccgcag
    58801 cgtagttggc ctgaccgcca tttcccatgg taccaaccac agaaccgata gtgataatac
    58861 gaccatgacg ctttttcatc atagcgcgca ttaccgcttt tgacagacgg aaaacagatg
    58921 aaaggttagt ttcgataata tcgttccact cttcatcttt cattcgcatt aacaggttat
    58981 cacgagtgat accggcatta ttgaccagga tatccacttc accaaattct gcgcgaattt
    59041 tttccagaac agattcgata gatgccgggt cggtcacatt caacatcaga cctttgccgt
    59101 tggcacctaa ataatcactg atcgcctgag cgccattttc actggtcgca gtgccaataa
    59161 ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg gccaattccg cggcttgcac
    59221 cggttaccag tgcgattttt ccttcaaaat tcatgatttt cctcttttaa agctcgagcg
    59281 ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt
    59341 tcgtcaggcc agtaagcacc ttgcccgggc cgacttcata gagatgttct acgccttgcg
    59401 ctgccatgta ctcaacagac ttcgtccact gaaccgggtt atacaactga cgtaccagtg
    59461 cgtcacggat ggcatcacca ttggtttcgc atttcacatc aacgttattc acaacagaaa
    59521 ctgttggtgc gttaaaggtg attttcgcta attctactgc cagtttgtcg gctgctggtt
    59581 tcatcagcgc acagtgagac ggtacgctca ctggtaacgg cagcgcgcgt tttgcgccag
    59641 cagctttaca ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca ataaccacct
    59701 gtcccggaga gttaaagttt accggagaaa cgacctgacc ttctgcagct tcttcacacg
    59761 ctttcgcaat aggcgcatca tccagaccga tgattgccgc catagcgccc gtgccttccg
    59821 gtacggcttc ttgcatgaac ttaccgcgca tctcaaccag acgcaccgca tcagcgaaat
    59881 caatcacgcc agcgcaaacc agcgcggagt attcccccag gctgtgaccg gccatcattg
    59941 ccggtgcttt accgccctgc tgctgccata cgcgatacag cgcaacagat gcagtcaaca
    60001 gcgctggctg ggtttgccag gttttattca gttcttcagc tgacccctgc tgggtcagcg
    60061 cccacaggtc gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg acaattggat
    60121 agctcgccgc catatcagcc agcattccaa cggtttgaga accctgtcca gggaacacaa
    60181 atgcaaattg cgtcatgttt taatccttat cctagaaacg aaccagcgca gagccccaag
    60241 tgaatccacc gccaaaggct tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc
    60301 gtacagcttc atccagcgcg cacgggacag aggccgcaga ggtattaccg tggcgatcca
    60361 gcgtcaccac gacattgtcc atcgacatac cgagtttttt cgccgttgca ctgataatac
    60421 gcaggttagc ctgatgcgga accagccagt ccagttgaga acggtcaaga ttattcgccg
    60481 ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag acttcgttgc
    60541 ccgccatcgt cagatgaatt gaattctctg gattcacgcg gtcggcgttt ggcagcgtca
    60601 gcaattcacc ataactaccg tcggcatgca gatgggtgga aatgattccc ggctcttcag
    60661 aggcagccag caccgcagcg cccgcgccat cgccaaaaat aataatagtc ccacgatctg
    60721 ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac ttcaccgccc
    60781 cagatttcac gtattgatcg gctacgctta atgcataggt gaaacctgca caggctgctg
    60841 caacgtcaaa tgccgggcaa cctttaatgt ccaacatgct ttgaatctga caagctgcgc
    60901 tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat caggccaatc tggtctttct
    60961 caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt
    61021 cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc cactcgtcag
    61081 aggtgtccac cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc
    61141 cagtaccaat aatcttcgta tacatgtacg ctcagtcact tttcggttat ataccgtcac
    61201 ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca gagttccgct tttgccaccg
    61261 tccagcagct caaaaccagc tgggtataca gattccaggc gagcggcaat tcgctgagga
    61321 acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg
    61381 gctgcaccat gactttttat caccgtgccg cgcaatccta acagacaggc gccgttatac
    61441 tggtcggggt tgaggtgact gaatcgcctc gtcaggctct tttgtagcca acgctttaat
    61501 aacagtagcc accacgaccg ttttttccct tcaccctgag atttcagcag agaaaggaac
    61561 atcctgacaa caccttccat cgtctttaac gtgacatttc ctgtaaagcc gtcacaaacc
    61621 agcacatctg tcttgccagt taacaactca ttggcttcaa gatagccgat ataattgata
    61681 gaagggattg ttttaagcac cgctgaggca tcccgaatac tgtcaagacc ctttacttct
    61741 tcttcaccaa tattgagcaa cgccacgcga ggattgggaa tttccaccac ctcttcagcc
    61801 agaactgagc ccataatggc aaattgcacc aacattgtgc tgtcacaatc gacgttggcc
    61861 cctaaatcga ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt caccagcgcc
    61921 ggacgctcaa tcccctccag gggcttgagt aataattttg ccagccccat cagtgccccg
    61981 gtattaccgg cactgacaca ggcttgcgct cgaccttctt tcaccagctc cagggccata
    62041 cgcattgaac tcccacggct ggcgcggata gcttgcgaag gccgggcatc actggcgata
    62101 actgactgcg caggaataat ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt
    62161 aatggcgtga tggcgtcggg attgccgact aaaagaagag tgagttgcga attagaattc
    62221 agtgcctgca atgctgcagg cactgtcacg gaagggccaa aatcccctcc catgacatct
    62281 aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt tccccagttg
    62341 cctggggaaa atcctcacta agcttcatca cgcagacgcg tgattactta gcgatgacct
    62401 tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt tcaccagaag
    62461 ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac
    62521 gtttggaacg ggttggttta ttctgttgta cggccatgga ccttactcct caattacggt
    62581 ttatccccga tccaacgggg agcaccaatt acttacgctt taagctggct aatacggcaa
    62641 atgggtttgg cttttgcact tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca
    62701 cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt
    62761 caaccattgc aagcagatcg atttcaccga attcgttaac ctcaatcggt tcatacgctt
    62821 ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact aaaacaatac gttgtgtaga
    62881 cctgatgagt aaacggcttc ccgcaacgct gacactcgag cgttaccgtc accttcgcat
    62941 cgccgtttaa cactgcgaga cgttggttat cgatagcgaa cgacatggag cattccacat
    63001 cactgtccac actgactacg gattcggcga cgcgctcaac ctgatcaggg gtatagatac
    63061 cctggtaatc aaggcgtttt tgagccgtac gaaccggatc gagagtcagg ggtaatttta
    63121 ccttttgcat agggcgcgca tattaacttt gtaacgtcat agagtcaaag aaaaaggcag
    63181 cctgtggttg ccttttgcca ataattcgca cacattgcgg gttactgctt tattttcgtt
    63241 cagtgctgat aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa tggctatcat
    63301 caatacgcta tatctgatgg aaaaaaatat gcctaaactt attttagcct ccacctcgcc
    63361 atggcgtcgc gctcttctgg aaaaactgca aatctctttt gaatgtgcag cacctgaggt
    63421 cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac tggcacaaga
    63481 aaaatcgcaa tctctggcgt cacgttatcc ggatcattta attattggtt cagaccaggt
    63541 atgtgttctt gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg cccgtctgca
    63601 attacgcaaa gccagcggca atatcgtcac cttctatact ggactggcgt tgtttaattc
    63661 ggcgaatggg catctacaaa cagaagtgga gccttttgac gtccatttcc gtcacctaag
    63721 cgaggcggag attgataatt acgtgcgtaa ggagcatccc ctgcactgcg cgggtagctt
    63781 taagagtgaa ggatttggca ttacgctgtt tgagcgttta gagggacgtg accctaacac
    63841 gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag ggaaaaaccc
    63901 gctgatggga tagttgtttg cgccggatgc ggttacgtcg catccggcaa tcggtgcagg
    63961 cggcctgatg cggcgtaaac gccttatcag gccaaccgcc cattatcagg cttatatgct
    64021 tgtattagcg cgcgttacgc agcttttgca aacaacgctt caaaccttca tccatcggcg
    64081 cttcgatacg catcacctca ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca
    64141 ggaacagacg atttaatccc gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac
    64201 cgtagcgatc gtcaaaggca atcggatgac ccgcatactg tgtatgcaca cggatctgat
    64261 gagtacgtcc ggttaccggg ctacaacgca ccagggtggc aaatgcatag cgttcttcca
    64321 ctttaaagcg tgtttccgac ggtttgcctt cctgactgac acgcacgata cgttcgccgc
    64381 tttgcagaat atttttcaat aacggcgctt gaacgctctt cacatgcgac tgccactgac
    64441 cgcgcaccag cgccaggtaa tctttttgca ttcctttttc acgtaattgc tcatgcagag
    64501 aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc tgaagtgtcc cggtcgagac
    64561 gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc
    64621 cgaagcttaa accactgccg ccatgtaccg ccgtaccgga aggtttattc agcaccagga
    64681 tatgatcatc ttcatataag atgacgtccg ccagcgccgc caccttttgc agatgtggcg
    64741 aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg
    64801 cttcgagttt atattcaggc ttaatacgtt ttttgttcac ccgcacttca cctttacgca
    64861 aaatacggta aatcatactt tttggtacgc ctttcaattg ggtacgcaaa aagttatcga
    64921 tacgttgccc cgcttcgtca gcggtgatag caacaatttt tacggatgga gtctctgttt
    64981 tcatggtggg cgattctaat tagccaacag gattcgcgcc actcattttt ctatgcttat
    65041 ttttagtttt gcaccttatt acttcactac gtgatcactt tattgatggt tattaaacca
    65101 atcaccagca agaagtgaaa aaactgtgag taagcgggtg ataaatggta aaagtcatct
    65161 tgctataaca aggcttgcag tggaataatg aggccgtttc cgtgtccatc cttgttaaaa
    65221 caagaaattt tacggaataa cccattttgc ccgaccgatc atccacgcag caatggcgta
    65281 agacgtattg atctttcagg cagttagcgg gctgcgggtt gcagtcctta ccggtagatg
    65341 gaaatatttc tggagagtaa tacccagtct gtttctttga taattgcgct gtttttccgc
    65401 atgaaaaacg ggcaaccgac actctgcgcc tctttgagct gacgataacc gtgaggttgg
    65461 cgacgcgact agacacgagg ccatcggttc acacccggaa aggcgttact ttgcccgcag
    65521 cttagtcgtc aatgtaagaa taatgagtaa gttacgatga aaagaatgtt aatcaacgca
    65581 actcagcagg aagagttgcg cgttgccctt gtagatgggc agcgtctgta tgacctggat
    65641 atcgaaagtc cagggcacga gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc
    65701 attgaaccga gtctggaagc tgcttttgtt gattacggcg ctgaacgtca cggtttcctc
    65761 ccactaaaag aaattgcccg cgaatatttc cctgctaact acagtgctca tggtcgtccc
    65821 aacattaaag atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga taaagaagag
    65881 cgcggcaaca aaggcgcggc attaaccacc tttatcagtc tggcgggtag ctatctggtt
    65941 ctgatgccaa ataacccgcg cgcaggtggc atttctcgcc gtatcgaagg cgatgaccgt
    66001 accgaattaa aagaagcact ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg
    66061 cgcaccgctg gcgtaggcaa atctgctgag gcgctgcaat gggatttaag cttccgcctg
    66121 aaacactggg aagccatcaa aaaagccgct gaaagccgcc cggctccgtt cctgattcat
    66181 caggagagca acgtaatcgt tcgcgccttc cgcgattatt tacgtcagga tatcggcgaa
    66241 atccttatcg ataacccgaa agtcctcgaa ctggcacgtc agcatatcgc tgcattaggt
    66301 cgcccggatt tcagcagcaa aatcaaactg tacaccggcg agatcccgct gttcagccac
    66361 taccagatcg agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct gccatccggt
    66421 ggttccattg ttatcgacag caccgaagcg ttaacagcca tcgacatcaa ctccgcacgc
    66481 gcgacccgcg gcggcgatat cgaagaaact gcgtttaaca ccaacctcga agccgccgat
    66541 gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat cgacttcatc
    66601 gacatgacgc cagtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt
    66661 caggaccgtg cgcgtattca aatcagccat atttctcgct ttggcctgct ggaaatgtcc
    66721 cgccagcgcc tgagcccatc actgggtgaa tccagccatc acgtctgccc gcgctgctcc
    66781 ggtaccggta ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg tttgatcgaa
    66841 gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt gccaatcgct
    66901 tcttatctgc tgaatgaaaa acgttctgcg gtgaatgcga ttgaaacgcg tcaggacggt
    66961 gttcgctgcg tgattgtgcc aaacgatcag atggaaaccc cgcactacca cgtgttgcgc
    67021 gtgcgcaaag gggaagaaac gccgacctta agctacatgc tgccgaagct gcatgaagaa
    67081 gcgatggcgc tgccgtctga agaagagttc gctgaacgta agcgtcctga acaacctgca
    67141 ctggcaacct ttgccatgcc ggatgtaccg ccagcgccaa ccccagctga acctgccgcg
    67201 actgtcgtag ccccagcacc gaaagctgca acggcaacac cagcagctcc tgcacaacct
    67261 gggctgttaa gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg tgaagaagcc
    67321 aaaccgaccg agcaaccaac accgaaagca gaagcgaaac cggaacgtca acaggatcgt
    67381 cgcaaacctc gtcagagcaa ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa
    67441 cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag
    67501 caaactgccg agacgcgtga gagccgtcag caggctgagg taacggaaaa agcacgtacc
    67561 accgacgagc agcaagtgcc gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt
    67621 caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa
    67681 caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa
    67741 gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg caccggtggt tgaagaaact
    67801 gccgctgccg aaccaattgt tcaggaagct ccagctccac gcacagaact ggtgaaagtc
    67861 ccgctgccag tcgtagcgca aactgcacca gaacagcaag aagagaacaa tgctgataac
    67921 cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt
    67981 ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc aatgccgttg
    68041 accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag tctggatccg ctatccaatt
    68101 gtacgtccgc aagatgtaca agttgaagag cagcgcgaac aggaagaagt acaagtgcag
    68161 ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac ctgttgttag cgcgccagtt
    68221 gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg ttgccgaacc gcaaccggaa
    68281 gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg taactgaaca gccgcaggtg
    68341 attaccgagt ctgatgttgc cgtagcccag gaagtcgcag aacacgcaga accgatggtt
    68401 gaaccgcagg aagagacggc agatgcagat attgaagaag ttgccgaaac tgctgaggtt
    68461 gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc tgaagtcgca
    68521 gcagaagttg aaacggtggc cgcggttgaa ccagagatca ccgttgagca taaccacgct
    68581 accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc gcgtcacagt
    68641 gactggcagc gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg tcatacggca
    68701 acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata attagctcaa
    68761 agtaatcaag ccctggtaac tgccagggct tttttatttc atgtttgaat caacatatta
    68821 aaattcatca aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa aaaatgcccg
    68881 ccccggcgat cccagagcgg gcagacaaaa catacccagt ttcatgatat gttttaaagc
    68941 gagcttattt gttgagctgg aagagcgaca atccctgcat atcggtaaat gctttatacg
    69001 atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat agttgcattc cagtccacat
    69061 caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct aaggaatcca
    69121 gcgactccag tttgttcagc tgcgtgccta attccgcgcg aacggtaagg acattattca
    69181 gcgagttttt cagtccacgg ttggttttat ccaatgccgc agcggcgatt tctttatccg
    69241 cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc gctatccagc atggcaaaaa
    69301 gattggtttc agaagcgcta ccgtctggtt ccgctaccgc gttactggta atactgtcga
    69361 aaattttgtc gcccgtatgt cctatcacca tcgaacgcga agcatcgacc tgttgcttaa
    69421 tactttctgt tccgccaaca taatcaccgt tagcctcact aaacggcgca gtctctgttt
    69481 tatatgtagc gcccattacc gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc
    69541 ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg
    69601 tagacaattt tttcctgagc attctggata gcagtggtga cctggctaag tacactctct
    69661 tccagtgaca ctttttgagt ggcgaaagta cgtgccagcg tgtactggct gttttgcgcc
    69721 tgtgcctggg agagaacaac agcttgtgat gcagcaatgg gatcgtctga agggttaacg
    69781 actcgcttac ccgtcgacat ctgctcgccg tacttcatcc attctgcctg agaattggtg
    69841 atgccgcgca tgttttgctg gtacatcatc tgggtactga aacgcatttt atctcccctt
    69901 agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg
    69961 ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg
    70021 aaatcgactg ctgctgattg gaaagctgcg tcaccacatt accttgcgtg gtgctactgg
    70081 ttttcaacgt cgcggtttta ttaccgatat cactcactaa cgaggcataa gcgtcgttaa
    70141 aggatttcgc accgcccacc gttttactgt tgctttgcag atccagcagg gcctgaccgt
    70201 tgcggttgtc gctatcaccc gcatcttctt cgctcgccat cgcgattttc gcttcgtcgg
    70261 tgattaatac atccatgttg acgatggcgt cacttactgg tttcagcgtg aagctgtcgt
    70321 taacggcagg cgttcctgta aacgtcaact ccagaccatc aaatgccact ttaccgttgg
    70381 catctggtgt cgccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc cactgattat
    70441 tatcgaacga gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt accgtggcac
    70501 cgatcgcaac gtcacctttg tttttcgtgt tttgcagaac cgcgggctta ccgatagtaa
    70561 agaaatcttc accagcatcg ccgttagcat caaatccggc tttgtgttgg ctgttgaaag
    70621 cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc agatcctgag
    70681 aacggaatgt cagaatgccg cccagcgacc cggtattcag taatttctcc gggatctcaa
    70741 tattgcctgc cgtcccatca acgtaagcga cagtcgtacg agaagggtcg gcgctggaag
    70801 gaactgccgc cagttgccgc gccgtacttc cctgaaccag tgagtaacca ttggcatcgt
    70861 gatgttataa gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc
    70921 gctcaccagt tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc
    70981 tgtcaggcgc gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg
    71041 atcaacgctg gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc
    71101 ggtggtttta aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgccgc
    71161 cgggtcttcc gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt
    71221 tgccagcgaa gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg
    71281 ggcggtcagg ccgctacttt gcgtctgcgc agcacgtaac tggttggtaa taaacgtatc
    71341 atactcacgc tgcacaccag aaacgtagac gccattgcca acccagccgc cagcgcccaa
    71401 cgtgctattg gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct
    71461 ggagatatta ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc
    71521 gttattgatc aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg
    71581 gcagcgaccc gccggacttg agttattcag aacagattat caatgttcat gctgtaggtt
    71641 ttgctcacct tgtcgcttat cgatttcatc tgctggatca tgttggtgag tttacgggca
    71701 tagtgaggat cggtagcata gcccgcgtcc tgtaggacct gcgccccctg ttccgcactc
    71761 gcggcggtcg tcacggcggc gtagcgcggg ttacgtgtta acagcccaac gtaatccgac
    71821 aatgcttcca gatacgagct gtagacccga aacttcgctt ttactttctt cgcttcgccg
    71881 ttttcatatt cagtcgtggt gatttcagtg acttgccctt tccagttgcc agaggctttg
    71941 acaccaaaca ggttatagct tggctcgccg ttttcgcggc ggatttgccg ttgcccccaa
    72001 ccagattcaa gcgcagcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt
    72061 gccagttggg cgggcagcga gagttgcgcg aggaatgctc tactgtcacc cggcagcgaa
    72121 tcatcgtagt tacgtggtac ggccttttgc accagctgcg aaagcgtctg gttttgataa
    72181 cgcaccacgg tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat
    72241 ggttgttctg gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc
    72301 gtcatctgtt gggcaatttg ctggtcatac atactggtat acagacgagt gtgctcgctg
    72361 ctgaacaggc catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac
    72421 atcccttcca cctgacgggc caccggacgg atatttgccg ccggatcttc gctcgctttc
    72481 gcctttagtt cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg
    72541 ctgatcatca gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca
    72601 aaatagacat cagatccatc ggcgtagcgc ccagcgcatt gagcgcacgc accacgttat
    72661 tgaggctggc gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct
    72721 gcgtttgtgg agtaacaaca gtctgtccac caccaaacgg tgtatctggc tgacattggc
    72781 ctgacgatta actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac
    72841 ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg
    72901 ggtgacatta acctgcatat tctggatatc ggcaaggaaa cggacctggg aactgttgcc
    72961 acttggtacg cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg
    73021 cacgcggttg atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg
    73081 caaattaagg gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc
    73141 attggtgatc cgtccaccgt tcagttggtt aacctgaaca ctgctaccgc ctgcggaggc
    73201 tcctgcgccg ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc
    73261 cttaagcgga gtcatcaaca acgtacctcc acgcaagctt ttggcattac ccatggaaga
    73321 aaccaccaca tcgatggttt gcccctgacg tccaaacgga ggaagtgacg ctgtcaccat
    73381 taccgcagcg acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg
    73441 tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccagt
    73501 gccatccagc cccactacca agccatagcc aatcagtgag ttttgcctta ccccctgaac
    73561 actggtgaga tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa
    73621 tgcagagaga aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa
    73681 cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaata
    73741 cgcgcatccg ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta
    73801 accacgcccg agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc
    73861 accacatgca ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta
    73921 aaggtattgc tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg
    73981 acatcggcac gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca
    74041 aaattagttt taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc
    74101 tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc
    74161 ggttgatagc catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc
    74221 gtcggaccgg gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc
    74281 caggcgcagc cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg
    74341 tttttttgca tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttat
    74401 gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt
    74461 gatttcgtaa gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt
    74521 agacgtttca acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc
    74581 gttcggtgca ccggaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagtcc
    74641 ggtatcattc ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc
    74701 ttgttgggtt acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg
    74761 aatggtgatc gctggctgca cctgaaaacc accagccgtc accagctgcc cgttctgatc
    74821 cacctggaaa gagccgtcac ggatataggc tgatgagcca tctggcaaca ttacctggaa
    74881 aaagccctgc cctttaatcg cgacatcttt gctgttattg gtctgcgaca gattgccctg
    74941 gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg ccgatttgta atccggaggg
    75001 taaggtggtt tgttcggaag actgtgcccc cggctggcga atggtttgat aaagcagatc
    75061 ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg acgtttgcca ggttgttggc
    75121 aatgacgtcc atattggttt gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact
    75181 gatcatataa tgtcctgtag tggattgctg acaagcagag cagaagcacg cgacagtgtg
    75241 aatttcccct caccctaacc ctctccccaa aggggcgagg gaatagtccg cgcgctttta
    75301 ttgcaatgtc atcaatcgtt caattaactc atcgacagca gttggttggc acggcctgcg
    75361 ttatcatcga cgctgctgat caccttcatc tgcatttcaa aacgccgcgc gctggcaatc
    75421 atgtcgctca ttgccgcaac ggcattgacg ttactgcctt ccagaacccc cgacatcaca
    75481 cgcaaggttg gatctgcctg cagtaccggc cctcgcgtgg cctgggtttc tgcgcttaaa
    75541 cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg tggctttcac cagtttcaga
    75601 cgccctactg gcgcaactgt atttgccgga tcgcccggat tgagcgccga gattgtgcca
    75661 tcggcagcaa tcgtgatttc cgccccttcc ggcacagcaa ttggccccgc ctcgcctatc
    75721 accggatgcc cctgaattgt cagttgcccg gtgggatcaa cctgaatgct gccattacgc
    75781 gtataccctt cgctgccgtc agcggtctgc acggccagcc atcctgctgc aacgcaacgt
    75841 ccagcgggcg agaggtgtaa tccatcttgc cgggcgtcat gtctgcaccc ggtgttgacg
    75901 ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac cggcactgcg cgcaaggcgt
    75961 ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag attactggcg gttaccgcct
    76021 gttgattcag cgtctggctg gctgctccca tcgcagtata aattgcgtga tccattgagc
    76081 tatcccgtca gcgattagcg taagttaacc cgcgtgttga ggatctggtc ctgggttttg
    76141 atggtctggg cgttagactt atagttacgc tgggcaacga tcatattgac cagttcttta
    76201 ctgagatcga cgttggacgc ttccagcgca ccgttggtca gagtgccaaa gtttcccgtc
    76261 ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac gttgtcgcct
    76321 tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc cagcagttgg
    76381 gtttgttcgt tggaattgtt gccgacaacc gtaccgtcat cattgatttg ataactcacc
    76441 agatcgcccg gtttgtagcc attctgggtg gttgccacaa tgttgttagc gccggttgcg
    76501 atattattcg ccatcgcacc atccactaat gtgccattag cattaaattc cagtgttgtc
    76561 gctgtcttcg caatgctgtt tggatcactg ctatcctggg tgtagacctg ccagttatta
    76621 tccccggtct tcacaaaata gacgctcatg tcatgagcat taccctgact gtcgaaaaca
    76681 gtcaccgaac cttttttgtt atagctatcc gcattgctgg cgctgaatgg cgtaacagta
    76741 ggaagcggat cactggaatt caggttgatc tgcatcgacg ccgtggtggt agttttcgct
    76801 gccatcaggg tattcgggat cgaaatattg gtcggattcg ccccttgctg aatagtcggc
    76861 ggcgtaccgg ttaccgggta acccgtcagc tgtaaacctt gcgtattcaa caggttacgg
    76921 ttttcatcca gcttaaattg tccgttacgg ctgtagaaca ccgaaccgtt gctgtctacc
    76981 agacggaaaa aaccgttctg gctgatagca acgtccagac ctcgcccggt gttggtggtc
    77041 gtgccatcgg taaagtcctg agtgataccg gcaactttta cccccagtcc cactttcgaa
    77101 ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa agccgtaggt ggcggagttg
    77161 gcgatattgt tgccaataac atcgaggttg gtggcggcag cgtttaatcc gctaaccgct
    77221 tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg
    77281 tcgagggtgg tggtgccgta agtgccgaga tccagcgtat taccgctgtt gccgcggatc
    77341 acaccctgca ccagagcaaa ctgcagcggc tgggcaacca gttgtgtacc accgttactg
    77401 gcgctaatcg ctacattgta agaaccgttc ggcgcagtgc tgccatcagt caacgaaccg
    77461 tcccaggtga aactgtgaac tccggcggtc agttcaccaa tatcaatggt gcgcacaacc
    77521 gcgccatttt tatcggtgat ggtggccgta actttgtctg ccgcctgttg cagctcaaca
    77581 ccaaacggcg tggtcgtggt cacagcccct tcttcactgc cggttcccgc aagaacagtt
    77641 gtgccgggga tcatcacgcc gtgaccgatc aggttactgg cctgtaacga ctggctgtta
    77701 tcaatctgtc cggaaataga tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg
    77761 ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca ttggattggt cgggtcctgg
    77821 tttttcagct gcgccaccag caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg
    77881 cccgtgagcg aactactgct ggtggtactg acgccggtat ttgtcggatc ggtggtggtt
    77941 accgcaatgg acatagcttt ctcctttatt gaccgagcgt aagggttttc agcatcatgc
    78001 ttttcaccgt gttgagcact tcaacattgg cctgatagct gcgtgacgcc gacatggtgt
    78061 taaccatctc cccgacgaca tcaacgttcg gcatttttat gtagcccttt gcatctgcca
    78121 gcggattacc tggttcataa accagtttgt ccggggcctg actttcttaa catcggcaac
    78181 ctttacgccg cctgtcgcag cacttggtgc agcgttaacc tggaatacca cctgttttgc
    78241 ccgatatggc tgtccatcag gaccggtcac gctatcagca tttgccagat tactggccgc
    78301 cacgttcagg cgctgggact gggcagttaa cgccgacccg gcgatatcaa aaatattcag
    78361 cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga tttgcccgct
    78421 caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg tgcgttcgcg
    78481 atccatatcg acggtattac cgtcaagcga aggctggtcc ggaatacggt attgcagttc
    78541 tgcggtagga ggcgtcagcg cctgcgccgg gatgtgctgc gttgaggtca tcgtcagtgc
    78601 aaccacactg gttgcatccc gtccacgttg catgactttt ttaagttcac tggcaaaatc
    78661 gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg ccagcacttc
    78721 ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg cgtcgagctt
    78781 atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat gcctttacgc
    78841 gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg atggtcattg
    78901 cggacaggta caattcacgt tgtagaaatg gctgggggcg aaaatgctga caataaaacg
    78961 tagcgtggcg atcatcgcga tactgttcag tccgttaagt acggcgagca atctcacatc
    79021 gcaattgcac aactttttta gcgcccaact cgcgggggta agtgatgagg ttcgtgtttc
    79081 tattcgtaca gcgcccaatc tactaccgcc atgcgagcag ccattgcttt cgatgagcaa
    79141 taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg acaaacgata
    79201 tttacaggtt aatgtgcagg ccacaggaaa ttatgtggtt gccgcgatgc ctattgtgcg
    79261 gggaggaaag ctggaagctg gcaatgtcaa actgaaacgt ggacgcctgg ataccctgcc
    79321 accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc gcgatctatc
    79381 acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa aagcgggaca
    79441 acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc aacgcagaag gtcaggcgct
    79501 gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg gtatcggaac aggtagtcag
    79561 cggcgttgtt gatgcagatg ggaatattct tataaacctg taagctgtta aagattaccc
    79621 gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg aataaaccat
    79681 gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc gttcaaccgc gcgaaaccac
    79741 cgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc gcctccacca gcaccagtgt
    79801 gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata tcaatcttga
    79861 acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg acaccggcaa
    79921 aattgccgat gcgctgatca acgaagcgca gcaagacttg cagagtaact gatcgtatga
    79981 cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc aaaacggtaa
    80041 tggatcaaga gcagcaacat ctctctatgg ggcagatcaa cggcagccag ttgcaatgga
    80101 ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag ttacgcaaga
    80161 aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa attactgtga
    80221 aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa ggacagattg
    80281 agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg ctatatgggg
    80341 cgaacggtca gacctcaaca ccccatcgcg gcagtaaaaa gatttcgatc tgaatattat
    80401 ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc aaattcttta
    80461 actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc cagcacgacc
    80521 gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg catgatatgt
    80581 aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg caacagaaac
    80641 gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca gtaaagcagc
    80701 gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccggcatg tttcaacgga
    80761 ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc aattttcact
    80821 ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca acactttcac tacaatcagg
    80881 ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc atcaaacgcg
    80941 gtaaatttac cgtactggaa cagcgaaacg gttaacggac ctgaaagaat gcccaacgca
    81001 accgcactcg gcagtgccaa caggaaacaa agacgcaacc cccagtccat caaacggttg
    81061 tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa aatggtgcca
    81121 agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta atacatccaa
    81181 gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa ggagatctgg
    81241 ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat tgctccagca
    81301 tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg cggtagctga
    81361 tacaccagct gcagaatgcc gccgaccgta acagcccacg ccagcgccag caccggtggg
    81421 ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag cagtgttggc
    81481 gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac cagcgacgcc
    81541 agggagatca gcaagatata gggaaaggta atctttagta gctggctggt cagggcaaat
    81601 ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg tgcggcgagc
    81661 atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac ataagagaca
    81721 aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat cggtacaaat
    81781 gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag tttaaaagcg
    81841 acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat tgcgtctcgt
    81901 gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag cgattttaat
    81961 aaattcatcg gtgttctaat ccggacccac gggcaaaacg cctgcaaggc aggcgctgaa
    82021 agaggcgaat agtctaccta gattatggtg aattgctacc gccagatgtt acagggttta
    82081 ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca ctgcctattc
    82141 gccggcggtt tgcggaactg tctgatttta cacgcattca atgaagtgcc gcgcacagcc
    82201 gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat tcactacacc
    82261 ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg caccgtcagt
    82321 cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacaag tggtgatctg
    82381 caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt cgttagtcag
    82441 tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg tatccaccac
    82501 atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacgc tattgctacg
    82561 atgtttatcc attcttagcg aggctgcggt ggcgagttgc gtttttaact caccgtagag
    82621 tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg ccgccagttc
    82681 caccagcctt tcagcatcgc gcagattttc tgccagcggt ttatcgacac agacatgtac
    82741 ccccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg agtgcacaaa
    82801 aaccgcatca caactggcgg caaggctgga taacgaatcg gcataaggaa tgcgccagct
    82861 gtcacaaatt ggcagggctt tggcgcgcgt aggcgaccag gctccttgta acgtccagtc
    82921 agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta atcccactac
    82981 gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa tcaagacgtt
    83041 gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggtctgtaaa tcaccgtcaa
    83101 ccatgttcga catagccgtt accgccggct gatcttcaac ctcaccactg aaaagatgca
    83161 tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa ggaccatctt
    83221 cgcgattcgc cagttgttcc agcgtcgact ccacttccgc catatcgctg aattcataca
    83281 ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc accacgcaat aacaacgtgg
    83341 tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc aaattctgaa ttacaaaaac
    83401 gttgctcata tttggtgacc cgattaccaa aaccgctcac tgtgcgtaga taatgacgtt
    83461 tgaccagatt atccagctgt tcctgcactt cggattcact cagattcatg accggttcac
    83521 ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcggg tattgctccg
    83581 gcgtggtcac ctgcttttcc agcaagcaac caattacgcg agcttccagg gcagtaagtt
    83641 gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt taatgccgtc
    83701 agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata gccttctttt
    83761 tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat ataattagcc
    83821 ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt cagggcttca
    83881 aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata gcaggcatga
    83941 aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa taatctcttt ttcgtccggg
    84001 tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat catccctagc
    84061 ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc ccagggcttg
    84121 aggaaatggc gattctctgc gtaataatcc gccagacgcc aggcatcacg atcatgcacc
    84181 agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact gcgatagcca
    84241 aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta ttactatacc
    84301 tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt cacatatttg
    84361 ataaaaactc tctatcaaag cgacgttttg ataaaaaaat actgtccccg aagtgatggg
    84421 aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc gggaggggaa
    84481 atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat cgataatatg
    84541 ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt cgttgatcaa
    84601 atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt tattcagcaa
    84661 ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc gatgattgtt
    84721 accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca cgaaccgtgg
    84781 ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga tccgccgcgt
    84841 tcggcgctgg tggtgaaact aatccgtcca caacaacgtg gtcgtttttt ctcgctgttg
    84901 atgatgcagg acagtgccag tgcggtcatt ggcgcattgt tggggagctg gctgttgcaa
    84961 tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctatg tgcggcgttc
    85021 aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt tcgcgaaggc
    85081 atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct ggcgggttac
    85141 tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga cgtggctggc
    85201 gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt aacgttgctc
    85261 taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt gatggctggg
    85321 ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggcct acaacaactt
    85381 ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc gcgtgaaacc
    85441 ttaagtgctt cgctggcgga cgcaagagct cgcggcagct atatggggtt tagccgtctg
    85501 ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt tgacctgggc
    85561 aaatcggcgc accagccaga gcttccgtgg atgatgctgg gaattattgg catcttcact
    85621 ttccttgcgc tgggttggca gtttagtcag aaacgcgccg cgcgtcgttt gctggaacgc
    85681 gacgcctgat ttgatactcc cccctgactc ctccatactg aaagtgaact gacagccaaa
    85741 tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct caaagcccgc
    85801 atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa cgacgacgca
    85861 gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa agatgacagc
    85921 cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga cggcaaaccg
    85981 ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt caatggctac
    86041 gtcaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt ttctactccc
    86101 gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt tgccgacctg
    86161 ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc gctggacaaa
    86221 ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat tcagctattt
    86281 ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg cgttgctaat
    86341 taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat ggcgatttaa
    86401 tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt tttttctgct
    86461 ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg cagatttcat
    86521 gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca gtggcctgct
    86581 cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta accagtcgct
    86641 tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg acgcccatat
    86701 tgttctgaca aacttgacca gccaaattgg tcgcgaagag ccgaataagg ttaccctaac
    86761 cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga ccatgaaact
    86821 gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat
    86881 ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct
    86941 tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt acgtcctgcg
    87001 cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa
    87061 gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc gcgcccttta
    87121 tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg tccggcaaaa acatcccttc
    87181 agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc gccgcccaga
    87241 cgactggcac cttcacctcc gcgatggcga catgttaaaa actgtcgtgc cgtataccag
    87301 cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga ccacagttga
    87361 ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc acgatttcac
    87421 cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg agcgcggatt
    87481 taatgaagcc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca ctaactccag
    87541 ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg
    87601 tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt ttgatcgtga
    87661 agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg cgctgaaagt
    87721 cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg
    87781 gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata tgctggttgg
    87841 tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc accaacaggc
    87901 attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg attctgcgcc
    87961 acatgcacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca acgccccaac
    88021 cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc actttgaagc
    88081 attctgttct gtaaacggcc cgcagttcta tggcttgccg gtcaacgaca cattcatcga
    88141 actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg acaccctggt
    88201 gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat cgtagcttcc
    88261 tgttgtcatt aggttatttt acctgtataa ataaccagta tattcaacag ggggctatta
    88321 tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct attgacgccc
    88381 tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa ggccacgtat
    88441 cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa gaagataaac
    88501 agcgcattag cgaaattctc caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg
    88561 aataatatgc agtgattttt gtttttgccg gatcgccccg gcttttttat tttacttacg
    88621 aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg ctaaaaaaat
    88681 atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt tatctgttcc
    88741 cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg cttcagataa
    88801 aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc catgatggaa
    88861 aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag caccgttgat
    88921 agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc cgagcaggaa
    88981 gggactgaag ttggtcagac gctctggtta accactgatg ttgccagaca atttatttcg
    89041 atattagaag caggaatcgc caaaattgaa tccggtgatt tcccggtaaa cgagtatcgg
    89101 cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc tttaccattt
    89161 ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag cagagagaaa
    89221 aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc cgccgggtat
    89281 tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc accgcatcaa
    89341 cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga aggcgaaaag
    89401 tatgtcccgt tggtcctccg cgcgcaaacg ctgtgggatg aactttcccg ccacaacgaa
    89461 gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga ctccgcattt
    89521 ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa gctcgatgcg
    89581 caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat cggcttattt
    89641 gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat ccaactggcg
    89701 aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg tcatgacgat
    89761 gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc gattgtctgc
    89821 gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt acgtaaagta
    89881 tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc ggcgtttacc
    89941 ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga cgcgttgaag
    90001 attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt tccgtttgcg
    90061 gaagtggcca gtgatgggtc agaagccttc ccgttcttgc gcaatgtatt gccgggtatc
    90121 ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga agattttatt
    90181 atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag tgggcacggt
    90241 tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga ccaaaaaagc
    90301 gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt acggcctcca
    90361 gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat ctcatcaata
    90421 atattcgcga gcatctgatg ctttatcttt ttctctgggg attgttggca attatggatc
    90481 tgatctatgt gttttatttt tagttttccg cactgacata aactgacaaa tatttaatct
    90541 ccctttcatt tagcgtgaaa aattgaatga gattattcct gcttctacag cactcaccag
    90601 acatctctga tgctgaaccc actaaactta tgaagattct ttcattatta attcaaaatt
    90661 attgaaataa aaataaatat cattcaattt gttatttttg tgttgcaatt cattctccga
    90721 cacgccattt tctttctctc gcaaattcgt attggatgca cttatgtcat tcacaaatac
    90781 ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa ttattgtcta
    90841 cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg gctggtatca
    90901 caaagcaccc gaactgcata aaagcatcgg tattttgtta atgatggggc tggttattcg
    90961 cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt cgcccatgac
    91021 tcgtcttgcc gcaaaagctg gacatctcgc attgtgccta ttgctgttcg ccatcggcat
    91081 cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg gctggtttga
    91141 cgtccccgcg acccttgccg acgctggcgc acaggcagac tttgccggtg ccctgcattt
    91201 ttggctcgcg tggagcgtcg tagtactgtc tgttatgcac ggatttatgg ccctgaagca
    91261 tcatttcatc gataaagacg acactctgaa gcgcatgctg ggaaagtcgt catctgacta
    91321 tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc
    91381 tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc acgcctttgt
    91441 taacttccgc atacagcatc ttggctatag ctggttatat ggcaccttta aagatttcga
    91501 cggtactttt acctttgatg aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa
    91561 caccaccagc gtcgatacta accacgccga acgcgataaa catcttcgca gtgcggattt
    91621 tctcaatacc acaaaatatc cacaggcaac attcacctcc accagcgtga agaaagacgg
    91681 tgacgaactg gatattaccg gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct
    91741 ggaagcgaaa ttaattggtc agggcgacga cccatggggc ggtaaacgtg caggctttga
    91801 ggccgaaggc aaaattaagc tcaaagactt caatatcaag acagatttag gtccagcttc
    91861 tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag agtgaaatgt
    91921 tgccggatgg cacgcatccg gcaatgatat ttattctgtt ggatcaggaa tacccagtgt
    91981 tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac gtcctgcccg
    92041 acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg cagcaaatct cactacaaca
    92101 acctttgtat ttttccgcgc atactggaca ctgaataaac agcaggtggc agccatcatt
    92161 tttacagttg gtatggctgt cgcacggcgc gccgcactgg tggcaatgcg cgataatctc
    92221 atccgatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac
    92281 cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccgccct cgatatgcca
    92341 gactttgttg aatccgttat gtttcatcca ggcactggcc ttttcacaac gaatgccgcc
    92401 ggtgcagtac atgacgattt ttttatcttt atgtgcctgc atcatctcga ctgcttttgg
    92461 cagctgctca cggaaggtat ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc
    92521 atactcatag tggttacgca tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt
    92581 cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag gatcgtcaat
    92641 accgtcggca acaatgcgat cgcgtacctt catgcgcagt acccagaagg atttcccgtc
    92701 atcatccaac gcgatattca ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg
    92761 cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg
    92821 cgccagatac actcgcccaa aaacattcag cgcggtaaac agctgatata aagcgtcacg
    92881 ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc
    92941 gctctcagcc aacattttgg cttttaacgc gtcgttggaa atgcggttgt gtaacactgg
    93001 catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat atagcaatta
    93061 tcgataatta acatccacac attttacgct acatttgcgc attaaaaatt atttgttatt
    93121 tacaagcgcg gcaatttcgc ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat
    93181 gcgacaatac atacaattgc ccgtataggt tgaaaaacag gattgatatg acgaatctac
    93241 ccaagttctc caccgcactg cttcatccgc gttattggtt aacctggttg ggtattggcg
    93301 tactttggtt ggtcgtgcaa ttgccctacc cgattatcta ccgcctcggt tgtggattag
    93361 gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac
    93421 tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat
    93481 ccgttggcat gggcctgatg gaaaccggta tggcgtggtt ctggccggac cgccgaatcg
    93541 cccgctggac ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg
    93601 gcatcctgtt agttggcatc cattttctga cactggagct gggcgcgcgg cagtttggta
    93661 tgcaggaacc ggggattggc gtttaccgcc cgaacgataa tccactgatt gactggctac
    93721 aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca
    93781 tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc accagatcat gattacggcc
    93841 cgcgctcaag cgttttcgtc ccgttatttg ccgttgagca ggctgcgacc acgaccggaa
    93901 cctggatgct ggcacggatg tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc
    93961 cagatggcaa agggtatcaa ttgattatgc tgccaccaga gtgttctccg ccactggatg
    94021 atgccgaaac taccgccgcg tggatgaaca aagtggtcga aaaatgcatc atgatggcac
    94081 cagagcagta tatgtggtta caccgtcgct ttaaaacacg cccggaaggc gttccttcac
    94141 gctattaaat ctcccatgcc ggatgcttca gaatggcatc cggcattacc acagcaaatc
    94201 cccctgattt agcgataaaa gctctctgga ttgcgccccc tggaagtcgg gcgcataatt
    94261 agtgtgttta tcttttcttc ttatgttcac cgcgcctggc gcaccaacag cggattgcta
    94321 tgtcaccctg tgaaaatgac acccctataa actggaaacg aaacctgatc gtcgcctggc
    94381 taggctgttt tcttaccggg gccgccttca gtctggtaat gcccttctta cccctctacg
    94441 ttgagcagct tggtgttacc ggccactccg ccctgaatat gtggtccggt attgtcttca
    94501 gtataatggg ttcctaaaca catcatttta ggtattgaat atgagacggg caatcagcta
    94561 catcagattt tcaagtgaac gacaactgaa aggggactct gtacgccgac aatctaagct
    94621 ggttactgac tggcttgata agaaccctga gttttacttg gattcatcct tgagctttaa
    94681 agatttgggg aaaagtgctt ttagtggtaa gcacctgaaa ggtggtctag gtgacttttt
    94741 aaccgctatc gagaaagggc ttgttaaagc tggtgatacg ctactaatcg agagtttaga
    94801 ccgtctgtca cgtcaggata ttgatattgc ttctgagcta ttacgccgta tcttaagggc
    94861 tggtgtagat gtagtcacac tgagcgatgg agaacattac acacgagaaa gtttaaaaga
    94921 cccgttagcc ctgattaaaa gcattctgat aatgcagcgg gcgcatgagg aaagtttgag
    94981 aaagtctgag cgtgtccaag ctgcatggaa ccgtaaaaaa gaactcatct ccgaaggtat
    95041 caaggtatct aggcgttgtc ctgcatggtt aaggctgaat gatgaccgtc gaacattcac
    95101 aatcattcct gataaagtgg aagttgttaa acgagcattt gacctgagat tacagggttt
    95161 aagcttttgg gctatcacca gaactttaaa cgatg
//
LOCUS       DAJTRH010000003        75662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTRH010000003 DAJTRH010000000
VERSION     DAJTRH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(436..642)
                     /locus_tag="OO339_000238"
     CDS             complement(436..642)
                     /locus_tag="OO339_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5925010.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            955..1044
                     /gene="kdpF"
                     /locus_tag="OO339_000239"
     CDS             955..1044
                     /gene="kdpF"
                     /locus_tag="OO339_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5925011.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1044..2717
                     /gene="kdpA"
                     /locus_tag="OO339_000240"
     CDS             1044..2717
                     /gene="kdpA"
                     /locus_tag="OO339_000240"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5925012.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2740..4814
                     /gene="kdpB"
                     /locus_tag="OO339_000241"
                     /pseudo
     CDS             2740..4814
                     /gene="kdpB"
                     /locus_tag="OO339_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4823..5395
                     /gene="kdpC"
                     /locus_tag="OO339_000242"
     CDS             4823..5395
                     /gene="kdpC"
                     /locus_tag="OO339_000242"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5925013.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5388..8068
                     /gene="kdpD"
                     /locus_tag="OO339_000243"
                     /pseudo
     CDS             5388..8068
                     /gene="kdpD"
                     /locus_tag="OO339_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8065..8750
                     /gene="kdpE"
                     /locus_tag="OO339_000244"
                     /pseudo
     CDS             8065..8750
                     /gene="kdpE"
                     /locus_tag="OO339_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            9025..9129
                     /locus_tag="OO339_000245"
     CDS             9025..9129
                     /locus_tag="OO339_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5925014.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            9440..11637
                     /gene="speF"
                     /locus_tag="OO339_000246"
                     /pseudo
     CDS             9440..11637
                     /gene="speF"
                     /locus_tag="OO339_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11634..12953
                     /gene="potE"
                     /locus_tag="OO339_000247"
     CDS             11634..12953
                     /gene="potE"
                     /locus_tag="OO339_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5925015.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(13009..14649)
                     /gene="pgm"
                     /locus_tag="OO339_000248"
     CDS             complement(13009..14649)
                     /gene="pgm"
                     /locus_tag="OO339_000248"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5925016.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14675..15220)
                     /gene="seqA"
                     /locus_tag="OO339_000249"
     CDS             complement(14675..15220)
                     /gene="seqA"
                     /locus_tag="OO339_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5925017.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15405..16169
                     /gene="ybfF"
                     /locus_tag="OO339_000250"
     CDS             15405..16169
                     /gene="ybfF"
                     /locus_tag="OO339_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5925018.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16309..16602
                     /gene="ybfE"
                     /locus_tag="OO339_000251"
     CDS             16309..16602
                     /gene="ybfE"
                     /locus_tag="OO339_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5925019.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16742..17272
                     /gene="fldA"
                     /locus_tag="OO339_000252"
     CDS             16742..17272
                     /gene="fldA"
                     /locus_tag="OO339_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5925020.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17482..17568
                     /gene="uof"
                     /locus_tag="OO339_000253"
     CDS             17482..17568
                     /gene="uof"
                     /locus_tag="OO339_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5925021.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17561..18007
                     /gene="fur"
                     /locus_tag="OO339_000254"
     CDS             17561..18007
                     /gene="fur"
                     /locus_tag="OO339_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5925022.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18091..18417)
                     /locus_tag="OO339_000255"
     CDS             complement(18091..18417)
                     /locus_tag="OO339_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925023.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18454..19860)
                     /gene="chiP"
                     /locus_tag="OO339_000256"
     CDS             complement(18454..19860)
                     /gene="chiP"
                     /locus_tag="OO339_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5925024.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20439..22103)
                     /gene="glnS"
                     /locus_tag="OO339_000257"
     CDS             complement(20439..22103)
                     /gene="glnS"
                     /locus_tag="OO339_000257"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5925025.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22306..24252)
                     /gene="nagE"
                     /locus_tag="OO339_000258"
     CDS             complement(22306..24252)
                     /gene="nagE"
                     /locus_tag="OO339_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5925026.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24585..25385
                     /gene="nagB"
                     /locus_tag="OO339_000259"
     CDS             24585..25385
                     /gene="nagB"
                     /locus_tag="OO339_000259"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5925027.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25445..26593
                     /gene="nagA"
                     /locus_tag="OO339_000260"
     CDS             25445..26593
                     /gene="nagA"
                     /locus_tag="OO339_000260"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5925028.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26602..27822
                     /gene="nagC"
                     /locus_tag="OO339_000261"
                     /gene_synonym="nagR"
     CDS             26602..27822
                     /gene="nagC"
                     /locus_tag="OO339_000261"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5925029.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27870..28622
                     /gene="nagD"
                     /locus_tag="OO339_000262"
                     /pseudo
     CDS             27870..28622
                     /gene="nagD"
                     /locus_tag="OO339_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            29019..30683
                     /gene="asnB"
                     /locus_tag="OO339_000263"
     CDS             29019..30683
                     /gene="asnB"
                     /locus_tag="OO339_000263"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5925030.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31062..31138
                     /locus_tag="OO339_000264"
     tRNA            31062..31138
                     /locus_tag="OO339_000264"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31096..31098,aa:Met,seq:cat)
     gene            31147..31231
                     /locus_tag="OO339_000265"
     tRNA            31147..31231
                     /locus_tag="OO339_000265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31181..31183,aa:Leu,seq:tag)
     gene            31255..31329
                     /locus_tag="OO339_000266"
     tRNA            31255..31329
                     /locus_tag="OO339_000266"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31287..31289,aa:Gln,seq:ttg)
     gene            31364..31438
                     /locus_tag="OO339_000267"
     tRNA            31364..31438
                     /locus_tag="OO339_000267"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31396..31398,aa:Gln,seq:ttg)
     gene            31454..31530
                     /locus_tag="OO339_000268"
     tRNA            31454..31530
                     /locus_tag="OO339_000268"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31488..31490,aa:Met,seq:cat)
     gene            31579..31653
                     /locus_tag="OO339_000269"
     tRNA            31579..31653
                     /locus_tag="OO339_000269"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31611..31613,aa:Gln,seq:ctg)
     gene            31691..31765
                     /locus_tag="OO339_000270"
     tRNA            31691..31765
                     /locus_tag="OO339_000270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31723..31725,aa:Gln,seq:ctg)
     gene            complement(31919..33094)
                     /gene="ubiF"
                     /locus_tag="OO339_000271"
     CDS             complement(31919..33094)
                     /gene="ubiF"
                     /locus_tag="OO339_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5925031.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33240..34664
                     /gene="miaB"
                     /locus_tag="OO339_000272"
     CDS             33240..34664
                     /gene="miaB"
                     /locus_tag="OO339_000272"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5925032.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34817..35857
                     /locus_tag="OO339_000273"
     CDS             34817..35857
                     /locus_tag="OO339_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5925033.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35854..36321
                     /gene="ybeY"
                     /locus_tag="OO339_000274"
     CDS             35854..36321
                     /gene="ybeY"
                     /locus_tag="OO339_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5925034.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36411..37289
                     /gene="corC"
                     /locus_tag="OO339_000275"
     CDS             36411..37289
                     /gene="corC"
                     /locus_tag="OO339_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5925035.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37314..38852
                     /gene="lnt"
                     /locus_tag="OO339_000276"
     CDS             37314..38852
                     /gene="lnt"
                     /locus_tag="OO339_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5925036.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39249..40157
                     /gene="gltI"
                     /locus_tag="OO339_000277"
     CDS             39249..40157
                     /gene="gltI"
                     /locus_tag="OO339_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5925037.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40327..41067
                     /gene="gltJ"
                     /locus_tag="OO339_000278"
     CDS             40327..41067
                     /gene="gltJ"
                     /locus_tag="OO339_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5925038.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41067..41741
                     /gene="gltK"
                     /locus_tag="OO339_000279"
     CDS             41067..41741
                     /gene="gltK"
                     /locus_tag="OO339_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5925039.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41741..42466
                     /gene="gltL"
                     /locus_tag="OO339_000280"
     CDS             41741..42466
                     /gene="gltL"
                     /locus_tag="OO339_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5925040.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42584..43519
                     /gene="rihA"
                     /locus_tag="OO339_000281"
     CDS             42584..43519
                     /gene="rihA"
                     /locus_tag="OO339_000281"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5925041.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43603..45273
                     /gene="hscC"
                     /locus_tag="OO339_000282"
                     /pseudo
     CDS             43603..45273
                     /gene="hscC"
                     /locus_tag="OO339_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45333..46784)
                     /gene="djlC"
                     /locus_tag="OO339_000283"
     CDS             complement(45333..46784)
                     /gene="djlC"
                     /locus_tag="OO339_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5925042.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46781..47488)
                     /locus_tag="OO339_000284"
     CDS             complement(46781..47488)
                     /locus_tag="OO339_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5925043.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47590..48144
                     /locus_tag="OO339_000285"
     CDS             47590..48144
                     /locus_tag="OO339_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5925044.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48154..49581)
                     /locus_tag="OO339_000286"
     CDS             complement(48154..49581)
                     /locus_tag="OO339_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5925045.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49578..50285)
                     /locus_tag="OO339_000287"
     CDS             complement(49578..50285)
                     /locus_tag="OO339_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5925046.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50449..51426
                     /locus_tag="OO339_000288"
     CDS             50449..51426
                     /locus_tag="OO339_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5925047.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51496..51978)
                     /locus_tag="OO339_000289"
     CDS             complement(51496..51978)
                     /locus_tag="OO339_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5925048.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52213..54795
                     /gene="leuS"
                     /locus_tag="OO339_000290"
     CDS             52213..54795
                     /gene="leuS"
                     /locus_tag="OO339_000290"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5925049.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54810..55391
                     /gene="lptE"
                     /locus_tag="OO339_000291"
     CDS             54810..55391
                     /gene="lptE"
                     /locus_tag="OO339_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5925050.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55391..56422
                     /gene="holA"
                     /locus_tag="OO339_000292"
     CDS             55391..56422
                     /gene="holA"
                     /locus_tag="OO339_000292"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5925051.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56424..57065
                     /gene="nadD"
                     /locus_tag="OO339_000293"
     CDS             56424..57065
                     /gene="nadD"
                     /locus_tag="OO339_000293"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5925052.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57089..57700
                     /locus_tag="OO339_000294"
     CDS             57089..57700
                     /locus_tag="OO339_000294"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5925053.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57960..58277
                     /gene="rsfS"
                     /locus_tag="OO339_000295"
                     /gene_synonym="rsfA"
     CDS             57960..58277
                     /gene="rsfS"
                     /locus_tag="OO339_000295"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5925054.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58281..58748
                     /gene="rlmH"
                     /locus_tag="OO339_000296"
     CDS             58281..58748
                     /gene="rlmH"
                     /locus_tag="OO339_000296"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5925055.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58779..60680
                     /gene="mrdA"
                     /locus_tag="OO339_000297"
     CDS             58779..60680
                     /gene="mrdA"
                     /locus_tag="OO339_000297"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5925056.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60683..61795
                     /gene="mrdB"
                     /locus_tag="OO339_000298"
                     /gene_synonym="rodA"
     CDS             60683..61795
                     /gene="mrdB"
                     /locus_tag="OO339_000298"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5925057.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61806..62894
                     /gene="rlpA"
                     /locus_tag="OO339_000299"
     CDS             61806..62894
                     /gene="rlpA"
                     /locus_tag="OO339_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5925058.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63033..64244
                     /gene="dacA"
                     /locus_tag="OO339_000300"
     CDS             63033..64244
                     /gene="dacA"
                     /locus_tag="OO339_000300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5925059.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64355..64618
                     /locus_tag="OO339_000301"
     CDS             64355..64618
                     /locus_tag="OO339_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5925060.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64719..65360
                     /gene="lipB"
                     /locus_tag="OO339_000302"
     CDS             64719..65360
                     /gene="lipB"
                     /locus_tag="OO339_000302"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5925061.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65619..66572
                     /locus_tag="OO339_000303"
     CDS             65619..66572
                     /locus_tag="OO339_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5925062.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66781..67746
                     /gene="lipA"
                     /locus_tag="OO339_000304"
     CDS             66781..67746
                     /gene="lipA"
                     /locus_tag="OO339_000304"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5925063.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67847..68050)
                     /gene="tatE"
                     /locus_tag="OO339_000305"
     CDS             complement(67847..68050)
                     /gene="tatE"
                     /locus_tag="OO339_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5925064.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68179..68967)
                     /locus_tag="OO339_000306"
     CDS             complement(68179..68967)
                     /locus_tag="OO339_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5925065.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69060..69443
                     /gene="crcB"
                     /locus_tag="OO339_000307"
     CDS             69060..69443
                     /gene="crcB"
                     /locus_tag="OO339_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5925066.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69497..69706)
                     /gene="cspE"
                     /locus_tag="OO339_000308"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69497..69706)
                     /gene="cspE"
                     /locus_tag="OO339_000308"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5925067.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69881..70441)
                     /gene="pagP"
                     /locus_tag="OO339_000309"
     CDS             complement(69881..70441)
                     /gene="pagP"
                     /locus_tag="OO339_000309"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5925068.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71030..72415
                     /gene="dcuC"
                     /locus_tag="OO339_000310"
     CDS             71030..72415
                     /gene="dcuC"
                     /locus_tag="OO339_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5925069.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72456..72797)
                     /locus_tag="OO339_000311"
                     /pseudo
     CDS             complement(72456..72797)
                     /locus_tag="OO339_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73135..73644)
                     /locus_tag="OO339_000312"
     CDS             complement(73135..73644)
                     /locus_tag="OO339_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5925070.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73807..74052
                     /locus_tag="OO339_000313"
     CDS             73807..74052
                     /locus_tag="OO339_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5925071.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            74027..75658
                     /locus_tag="OO339_000314"
                     /pseudo
     CDS             74027..75658
                     /locus_tag="OO339_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18622 a        19002 c        19216 g        18822 t
ORIGIN      
        1 acataacatc ttattggatt agatatgtta acaattaatt attcttaata tatcaataaa
       61 gtatatatta ctctctttac tatctgtttt tattgcggta gcgatactaa taacaaaatt
      121 cggaatttat ttagatgctg gaattagaaa aagggatgac ggctttctca ccttaccctg
      181 aagtttcacg aaagtgtgta aggaagtatg caatctaact ggagatttct gatgatctag
      241 tcgcttagag cgacaatgaa ggtgaccgca tcctgggtag ccctatgaaa ggatactatt
      301 gaaacgtgat gctaaaacag tgatgaaatg aataatacga tggaaacgtc ttccatgtta
      361 attatcatgg aagacgttga tgttgaacac ctaaaccagc cccctgatac gaggctggct
      421 taagcgcgca ggcaatcagt aaaaatcacc ggttgccttt tcggcctgat ccatccacac
      481 cggtttatcg ctggttttcg accagacgcg gtgcaggtag ctgtaaaagc gggcgcggtc
      541 tttccagaaa agcatgaaag gcagcgccag aacgcccgct gcaaccgcat atgtacggcg
      601 taaaaagata agccatgcag gatattctct gtagagttcc atacttttct cccccttttg
      661 tatctacccg gtgaatagca ccggaaaaat gaatttgttt atctgatgaa aatagtaccg
      721 ccttttgtgt aattttacta ctcatccgac cacttatttt tgcttattga tggtttattt
      781 acattcacac tgtaattaaa ttacacaaaa gttaaattaa tactaaccat cagttaaatc
      841 atggcctttg ccatttttat acttttttta caccccgtcc gcagattttt gcgaaatctt
      901 tgcagccaga attctactct tccggtatca cttttaggcc actggaggtg cactgtgagt
      961 gcaggcgtga tagccggcgt gttgctggtg tttttattac tgggctattt ggtttatgcc
     1021 ctgatcaatg cggaggcgtt ctgatggctg cgcaagggtt cttactgatt gccacgtttt
     1081 tactggtatt aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg
     1141 acattcctct tccaggtaca gcgggcgttg aacgtatact ctttcgttta cctggcgtct
     1201 ctgaccatga gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg
     1261 ggctggcggt gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc
     1321 agttgccggg gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata
     1381 ccaactggca atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcaggct
     1441 taacggtaca aaactttctt tctgccgcca gcgggattgc ggtgattttt gccttcatcc
     1501 gtgcgtttac tcgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca
     1561 tcacgttatg ggtgctagtc cctgtggcgt tgttgatcgc actgtttttt attcaacaag
     1621 gtgcgcagca aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc
     1681 tgttacccat gggaccagta gcttctcagg aagcgatcaa gatgctcggt actaacggcg
     1741 gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact
     1801 tcgtgcagat gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag
     1861 tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca
     1921 tctgcgtagg cgtggttatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg
     1981 gcgcagacag cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta
     2041 gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg atgcatgatt
     2101 cgtttaccgc tctcggcggc atggtgccga tgtggctaat gcaaattggt gaagtggtgt
     2161 tcggcggtgt cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta
     2221 ttgccgggct gatgattggt cgtacaccgg aatatctggg taaaaaaata gacgtacgcg
     2281 agatgaaact gactgcactg gcgattctgg ttactccaac actggtactg atgggcgcgg
     2341 cgttggcgat gatgaccgac gccggacgta gcgccatgct taaccctagc ccgcatggtt
     2401 ttagcgaagt gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc gcctttgccg
     2461 gattaagcgc caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc
     2521 gcttcggggt gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc
     2581 aacccgccag ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg
     2641 gcaccgtgtt gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg
     2701 cggaatatct ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg
     2761 ctattcgaac caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg
     2821 caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc
     2881 tgtattagca tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca
     2941 attagcggct ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa
     3001 ggccgcagta aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgcccgc
     3061 aagctgcgtg agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt
     3121 aaaggtgaca tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt
     3181 gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt
     3241 gaatccggcg gcgattttgc ctccgttacc ggcggcacgc gtattctttc tgactggctg
     3301 gtgattgagt gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg
     3361 gaaggcgcac agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct
     3421 ctgaccatcg tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt
     3481 aatgccgtga gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt
     3541 ggtggcctgt tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg
     3601 attgccacca gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat
     3661 aaaaccggca ccatcacact cggtaaccgt caggcgtcgg agtttatccc cgcgcagggc
     3721 gtggatgaaa aaacgctggc tgacgccgca caactggctt cgctggctga cgccgcacaa
     3781 ctggcttcgc tggctgatga aacgccggaa ggtcgcagta ttgtgatcct cgccaagcaa
     3841 cgttttaacc tgcgcgagcg cgatgtgcag tcgctccatg ccacctttgt accgtttact
     3901 gcgcaaagcc ggatgagcgg gatcaacatc gacaaccgca tgatccgtaa aggttctgtc
     3961 gatgccattc gccgccatat tgaggctaac ggcggtcact tccctgccga tgttgatcaa
     4021 aaagtcgatc aggttgcgcg tcaaggagcc acgctgctgg tggtggtgga aggttctcgt
     4081 gtgctgggcg ttattgcgct gaaagatatc gtcaaaggcg gtattaaaga gcgcttcgcc
     4141 cagctgcgca aatgggcatt aaaacggtga tgattaccgg cgataaccgt ctgactgccg
     4201 ccgcgatagc tgcggaagcg ggtgtcgatg attttctcgc cgaagcgaca ccggaggcca
     4261 agctggcatt gatccgtcag tatcaggcgg aaggtcgttt ggtagcgatg accggcgaca
     4321 gcaccaacga tgctacggcg ctggcgcagg cagatgtcgc ggtggcgatg aactccggca
     4381 cccaggcggc taaagaggcg ggcaatatgg tggatctcga ctctaacccg accaagttga
     4441 tcgaggtggt gcacattggc aaacagatgc tgatgacccg tggctcgctg accaccttca
     4501 gcattgccaa cgatgtggcg aaatacttcg ccattattcc ggcggcgttc gcggcaacat
     4561 atccgcagtt aaatgcgctg aacatcatgc gcctgcattc gcccgactcc gccattctca
     4621 gtgcggtcat tttcaacgcc ttgattatcg tctttttgat tcctctggcg ttaaaaggcg
     4681 tgagttataa accgcttacc gcttctgcca tgttgcgccg taacttatgg atttacggtc
     4741 tgggtgggct gctggtgccg tttattggta tcaaagtcat tgatttactg ctaaccgttt
     4801 gtagtctggt gtgaggttta ccatgagtgg attacgtccg gcattatcga catttctctt
     4861 tctgttattg attactggcg gcgtttaccc gctgctgacc accgcactgg ggcaatggtg
     4921 gtatccctgg caggccaacg gttcgttgat tcgtgaaggt gatacggtgc gcggttcggc
     4981 attaatcggg caaaatttta ccggcaacgg ctattttcat ggtcgcccgt cggcaacagc
     5041 agaaatgccc tataatccac aggcttctgg cgggagcaat ctggcagtca gtaacccgga
     5101 gctggataaa cagatagccg cacgcgttgc cgcattacgg gccgctaacc cggacgccag
     5161 cacgaacgtt ccggttgaac tggtgacggc gtcggcaagc ggactggaca ataacatcac
     5221 cccgcaagcg gcggcctggc agatcccacg catcgcaaaa gcgcgtaatc tcagcgttga
     5281 acagctcacg cagctgatcg caaaatacag ccaacaaccg ctggtgaaat atatcggcca
     5341 gccggtcgtc aacattgtcg aactcaatct ggcactggat aagcttgatg aataacgaac
     5401 ccttacgtcc cgaccccgac cgtctgctgg aacaaactgc cgcgccgcat cgggggaagc
     5461 tgaaagtttt cttcagtgcc tgtgcaggcg tcgggaagac ctgggcgatg ctggcagaag
     5521 cccagcgact gcgggcgcaa gggctggata ttgtggttgg tgtggtagaa acccatgggc
     5581 ggaaagatac cgccgccatg cttgaagggc tggctgttct gcctctgaaa cgccaggcgt
     5641 accgtgggcg gcatatcagc gagtttgatc tcgatgccgc cctcgcccgc cgtccggcgc
     5701 tgatcttaat ggacgaactg gcgcacagta atgcgccagg ttctcgtcat cctaaacgct
     5761 ggcaggatat cgaagaactg ctggaagctg gcattgatgt tttcacaacc gtcaacgttc
     5821 agcatctgga aagtctgaat gatgtggtca gcggcgtcac cggaattcag gtacgggaaa
     5881 ccgtgcccga tccttttttc gatgccgccg acgacgtggt gctggtggac ttgcccccgg
     5941 acgatctgcg ccagcggctg aaagaaggca aagtctatat tgccgggcag gcggagcgcg
     6001 ccattgaaca ttttttccgc aaaggcaatc tgatcgccct gcgcgaactg gcactgcgcc
     6061 gtactgccga tcgcgttgat gagcaaatgc gcgcctggcg ggggcatcct ggcgaagaga
     6121 aagtctggca cacgcgcgac gcgatccttt tatgcatcgg ccataacacc ggcagcgaaa
     6181 actggtccgc acggcggcgc ggctgacgtc acggctgggg agcgtctggc acgcggtgta
     6241 tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa cgtcgggcaa ttctcagcgc
     6301 cttacgtctg gcgcaggagc tgggcgcgga gacggcaaca ctttctgatc cagcggaaga
     6361 gaaagcggta gtgcgttatg cccgtgaaca taatctcggc aagattattc tcggtcgccc
     6421 agcctcgcgc cgctggtggc gtcgggaaac atttgctgac cgactggcgc gcatcgcccc
     6481 cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc gcccgcacga ttaacaacgc
     6541 gccggatagc cgctcattta aagacaagtg gcgggtacag attcagggat gcgtggttgc
     6601 cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag tggctgatgg cgtttgatgc
     6661 cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg gcgctatttt atggacgctg
     6721 gccttcggtg gttgccaccg tcattaatgt ggtgagtttc gatctctttt ttatcgcccc
     6781 acgcggcacg ctcgccgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac
     6841 cgtcgggctg gtgatcggga accttactgc tggtgtgcgt tatcaggcgc gggtagcccg
     6901 ttaccgcgag caacgcacac ggcacttata tgaaatgtcg aaagctctgg cggtgggccg
     6961 cagtccgcag gacatcgccg ccaccagcga acaatttatt gcctccacgt ttcatgcccg
     7021 cagtcaggtg ttgttgcccg atgacaacgg taaattgcag ccgttaacac atccgcaagg
     7081 aattacgcca tgggacgatg ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg
     7141 cgcgggcacc gacacgttac ccggtgtacc gtaccagatt ttgccgctaa aaagcggcga
     7201 gaaaacctac gggctggtgg tggtggagcc ggggaatctg cgccagttga tgatcccgga
     7261 acagcagcgc ctgctggaga cttttacgct gttagtcgcc aatgcccttg agcggctgac
     7321 gctaaccgcc agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc
     7381 cctgctggca gcgctttcgc atgatttacg cacgccgctt acggtgctgt ttggtcaggc
     7441 agaaatctta acgctcgatc tggcaagcga aggatcaccc cacgcccgcc aggccagcga
     7501 gattcgtcag catgtgctga acactacccg actggtgaat aatctactgg atatggcgcg
     7561 aattcagtcc ggcggcttta atttgaagaa agagtggtta acgctggaag aagtagtcgg
     7621 cagcgcgctg caaatgccgg aaccgggttt atcgtcgccc atcaatcttt ctctgccaga
     7681 accgctgacc ttaatccacg ttgacgggcc actctttgaa cgggtgctga ttaatctgct
     7741 ggagaacgcg gtgaaatatg cgggtgcgca ggccgaaatt ggtatcgatg cccacgttga
     7801 gggcgaaaat ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga
     7861 gcagacgata tttgataagt ttgctcgcgg gaataaagag tcggcggtgc cgggtgtcgg
     7921 gcttgggttg gcaatttgtc gggcgattgt ggatgtacac ggcggcacca ttaccgcgtt
     7981 caaccgaccg gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ccgctcctga
     8041 acttgaagac tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct
     8101 attcgtcgct ttctgcgcac ggcgctggag ggcgacggga tgcgcgtctt tgaggccgaa
     8161 actctgcaac gcggcttgct ggaagcggca acccgtaagc cagatttgat tattctcgat
     8221 ctcggcctgc ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcccg
     8281 gtgccggtga ttgtgctttc cgcacgcagc gaagagagcg acaagatcgc cgcgctggat
     8341 gccggagcgg atgattatct gagtaagccg tttggtattg gcgaattgca ggcccgtctg
     8401 cgcgtcgctt tacgccgcca ctccgccact ccgccaccgc cgcgcccgat ccgctggtga
     8461 aattttccgg tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg
     8521 tgcatctcac accaattgag ttccgcctgc tggcggtgct gctcaacaat gccggaaaag
     8581 tactcaccca gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc
     8641 actatttgcg tatttatatg ggacatctgc gacaaaaact ggaacaggat ccggcccggc
     8701 ctcgccactt cattactgaa accggtattg ggtatcggtt tatgctttga attgttattc
     8761 aataaattcg cttaaatagt gattaattta ttattaaacc tgcgccgaat attattattt
     8821 ggcaggttta atttcttaac gcaaatataa aacacgaaat tacaacattt aagtaacaaa
     8881 acagcatcta atatggcgat attcataatt aatgatttat tatctggggt atgatcgttt
     8941 tttgttgatc ttcttcacag attatagcca tttcatggat agaataactc taccttcaac
     9001 tgacacagca agcggtaaag gtaaatggaa aataataacc gcttaatgcc tcatataagg
     9061 cggacaaccc atatcatgaa gtttacccat cgtaatagtt ttgactttca tttctttaat
     9121 gcccgctagt cttctgacta aagggcaccc caacgtacag gtctccctga ctttaagcat
     9181 tacaggttaa tacctgtatt cctcggtgct catatactgc taaccctttt aaactctaaa
     9241 taattcgagt cgcagcactt gcaacttgag gtatgacgag tatagccagt taccgggctg
     9301 gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt atttatcagc ggtggagctt
     9361 tgcttttttt ccgacgtgat cgatttctcc tttgagaaat tgaggacctg ctattacctg
     9421 aaataaagag atgaataaaa tgtcagaatt aaaaattgcc gttagccgtt cttgcccgga
     9481 ttgtttttcc actcatcgtg catgcgtgaa tattgacgaa agtaattata ttgacgttgc
     9541 cgccattatt ttatcagtca atgatgttga acgtggaaaa ctcgataaaa tagacgctac
     9601 tggctatggc attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac cagcagaata
     9661 tctgccacgt atttctggtg tcttcgagca ttgcgaatca cggaaagagt tttatggacg
     9721 tcagttagaa accgctgcta gccattatga aacgcaactg cgcccgccct tcttccgcgc
     9781 actggtcgat tatgtcaatc agggcaatag cgcttttgac tgcccgggtc atcagggagg
     9841 tgagtttttc cgtcgtcatc cggcagggaa tcagttcgtg gagtacttcg gagaaacgct
     9901 gttccgttcc gacttatgca acgcagacgt agcaatgggc gatctgctga ttcacgaagg
     9961 cgcgccatgc attgcacagc aacatgcggc aaaagtgttt aatgccgata aaacatactt
    10021 cgttctaaat ggcacttcat cttctaacaa agtggtttta aacgccctgc taacaccggg
    10081 tgatctggtg ctgtttgatc gcaataacca caaatctaac caccacggag cgttgctaca
    10141 ggctggtgca acaccggttt atctggaaac ggcacgtaac ccgtatggct ttatcggtgg
    10201 cattgatgcg cactgttttg aagaaagtta cctgcgtgag ctgatcgcgg aagtcgcacc
    10261 gcagcgggca aaagaggctc gtcctttccg cctcgctgtg attcagttag gcacctacga
    10321 cggtacgatt tataacgccc gccaggtggt ggataaaatt ggtcatctgt gtgactacat
    10381 tctgtttgac tccgcatggg ttggctatga gcaatttatc ccgatgatgg ctgactgttc
    10441 gccgctgttg ctggatctta atgagaacga tccgggtatt ctagttacgc aatccgtaca
    10501 taaacagcag gctggttttt ctcaaacttc acaaatccat aaaaaagaca gccacatcaa
    10561 agggcaacag cgttatgtac cgcacaaacg catgaacaac gcctttatga tgcacgcctc
    10621 caccagcccg ttctatccgc tgtttgccgc actggatatc aacgccaaaa tgcatgaagg
    10681 tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat ggcattaatg cccgcaaact
    10741 gatcctcgat aactgtcagc atattcgtcc gttcgtacct gaactggtgg atggtaaacc
    10801 ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc agtttgtacc
    10861 aggggaacac tggcattctt ttgaaggcta tgcagagaat caatactttg tcgatccttg
    10921 caaactgttg ctgacaaccc caggtattga tgcacgtaac ggcgaatatg aagcgttcgg
    10981 tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc cggaaaaatg
    11041 cgatcttaac gccatcctct tcctgctgac tccggcagaa gatatggcca aacttcagca
    11101 acttgtgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa
    11161 gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac cctgcgtcag
    11221 ttgtgccagg aaatgcatga tttgtatgcc cgccacaacg tgaaacaact gcaaaaagag
    11281 atgttccgca aagcgcactt cccacacgtc agcatgaatc cgcaagaagc caactacgcc
    11341 tatttacgcg gtgaagtgga gctggttcgt ctaccggatg ccgaaggtcg tatcgctgcc
    11401 gaaggggcgc ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga aatctggggt
    11461 ggtgctgttt tgcgttactt cagcgctctg gaagaaggga tcaacctgct gccaggtttt
    11521 gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc
    11581 tatgtcatca agcctcgtga tgcgcaaagc accctgttga aaggggaaaa attatgagtc
    11641 aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat actgacgatg gtcaacatga
    11701 tgggctccgg tatcatcatg ctgccgacaa agcttgccga agttgggaca atttcaatta
    11761 tctcctggct ggtgacagcc gtaggctcaa tggcactggc atgggcattc gcaaaatgcg
    11821 gtatgttcag ccgtaaatca ggtggtatgg gcggttatgc cgagtatgct ttcggtaaat
    11881 ccggtaactt tattgcgaac tatacctacg gcgtctcact gctgattgct aacgtcgcga
    11941 ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg tcgccagtgc
    12001 agattggtct tgcgaccatc ggggtgctgt ggatttgtac cgtggctaac tttggtggtg
    12061 cgcgcattac cgggcaaatc agtagcatta ccgtgtgggg gggcattatt ccggtggtag
    12121 gcctgtgcat tatcggctgg ttctggttta gcccgacgct ttacgttgat tcctggaatc
    12181 cgcatcatgc accgttcttc agtgcagtag gttcttccat cgctatgacg ctgtgggctt
    12241 ttcttggtct ggagtctgcg tgtgcgaata ctgatgtagt ggaaaacccg gaacgtaatg
    12301 tgccaatcgc ggtactcggc ggtacgttag gtgcggcggt gatttatatc gtctccacca
    12361 acgtgattgc agggattgtg cctaatatgg agctggcaaa ttcaacggca ccatttggcc
    12421 tggccttcgc acagatgttt acaccagaag tgggtaaagt cattatggcg ctgatggtga
    12481 tgtcctgctg cggttcgctg cttggctggc agttcaccat tgcccaggtg tttaaatctt
    12541 catctgatga aggctacttc cctaaaattt tctcccgtgt aaccaaagtg gatgcaccgg
    12601 tgcagggaat gttgaccatt gtgattatcc agagcgggtt ggcgctgatg acgattagcc
    12661 cgtcgctaaa cagtcagttc aacgtgctgg ttaacctggc cgtggtgacc aatatcattc
    12721 cgtatattct gtcaatggcg gcattagtca ttattcagaa ggtcgctaat gtgcccccct
    12781 caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc gatgtatagc ttttatgcgc
    12841 tgtactcatc cggggaagaa gccatgctgt acggttccat cgtgaccttc cttggttgga
    12901 cactgtatgg tctggtatca ccacgctttg aactgaaaaa taaacacggt taaaaaagca
    12961 aagtaaaaaa gggcgatctt gcgaccgctc tttttttatt aaatgtgttt acgcgttttt
    13021 cagaacttcg ctaacaatct caaccgcttc tttctcaatc tgcttgcgat gttcttcacc
    13081 gaggaagctt tcgcagtaga tcttatatgc gtcttccgtg cctgacggac gcgcggcgaa
    13141 ccagccgttg tcagtcatca ctttcagacc gccaatagaa gcaccgttgc ccggagcagc
    13201 agtcaggcgc gcggtgatcg ggtcacctgc cagggtgctg gcgctcacca tttccggaga
    13261 cagcttagac agcgccgctt tttgtgcgga agtcgcagct gcctgcaaac ggttgtagct
    13321 cggcgcacca aagcgttttg ccagttcgtt gtagtgttcc tgcgggttct taccggtgac
    13381 agcggtgatt tccgccgcca gcagacacat aatgatgccg tctttgtcgg tggaccacgg
    13441 cgtgccgtcg aaacgcagga aggaagcccc tgcgctctct tcgccgccaa agccgaagct
    13501 gccgtcgaac agaccatcaa caaaccattt gaaacctacc ggtacttcta ccagcttacg
    13561 gcccaagtca ttgaccacac ggtcgatcat cgcagaagaa accagcgttt taccgacagc
    13621 aacatctttg ccccactgcg gacgatgctg gaacaggtag ttgatcgcca ccgccaggta
    13681 gtggttcgga ttcatcaaac ctgccggagt gacgataccg tgacggtcat aatccgggtc
    13741 gttagcaaac gccagatcga acttatcacg cagtgccagc agacccgcca tcgcacactc
    13801 ggaggagcag tccatacgga tcgcaccgtc tttatcgagg tgcataaagc ggaaggtttg
    13861 atcgacctga tcgttaacga tggtcaggtt gaggttgtaa tactcgccaa tgcgcttcca
    13921 gtattcgata ccggaaccgc ccaacggatc aacgcccagc gtcaggcccg ctttctgaat
    13981 cgccgccata tcaacgatat cggccagccc ttccacgaac ggctgcacca gatcctgctc
    14041 tttcacatag ccggatgcca tcgcttcgtc gagggagata cgcttcacgc ctttcaggcc
    14101 atcggccatc agtgcgttgg ccttgtcttc caccactttg gtgacgttag tatcagccgg
    14161 gccaccattt ggcggattgt acttgatacc accatcttcc ggcgggttat gggacggtgt
    14221 aatcacgata ccgtctgcca gcgggccacc ttttttattg tgaaccagga tggcattgga
    14281 aacggcaggc gtcggagtga agccattgtt ttcctgcaca atgacatcaa cgccgttcgc
    14341 tgccagcact tccagtacgg aaatgaacgc aggctcggac agggcgtgag tatctttacc
    14401 cacatagcaa gggccagtga tgccgttttt cgcacgttct tcagcaattg cctgagcgat
    14461 cgccagaatg tgcggctcgt taaagctgtg gcgcgctgca ctgccacggt gaccggaagt
    14521 accgaatttc accgcgtgct ccgcattccc tgcttctggt ttcagtacat aatattgcgc
    14581 cgtcagttgg gcgacgttaa tcaaatcact ctgttgtgca ggttggcctg cacgattgtg
    14641 gattgccatt gctttgtcct ttgtctgcaa cgttttagat agttccgcaa accttctcaa
    14701 tcaattccgc cgggaattgc atcgactgca tgatgtgttc gatcatgctg catttacggc
    14761 cggtgttggt gttggtgatc acccaatacg gcgtgcctgg cacatgtttc ggcttggtct
    14821 gattaccatt tttcagcagc gtttgttcat ctgccgcaaa gtacacgcgt gtacgaccgt
    14881 gcaacgattc cgttgcttcg gcaaacgcct gggcgtcaag agaatatagt gtagacaaca
    14941 gcagcataaa gcgattgacc gctcgctttt gctctgcgta ttcgtccgaa agcagaagtt
    15001 cacgcattgc gcgaaccttg tctttaatcg ttttgaccgg cttcgcttcg acgatagcag
    15061 gtgacgcaac gcgaacctct ttcgtcaccg gagcagcagg ctgtgatgcg gcggaaaatt
    15121 tcaacatacg ccgtaaaatg tcggatgcgc tctcgccgat atgcttagtg tggctggcaa
    15181 tatagctgta gagttcatca tcaacttcaa tcgttttcat cttaatccag tgcagtgtct
    15241 tagctgaata caagtcgcca agagtatatg aataaatccc ggcagcggat agcgtcaagc
    15301 ctggcttgca gaaaaactcg gaataaacct tatttgttgc agccctgtgg cacaatggtc
    15361 aacataatac cctaacccga caacgcgaaa aaaagaactt tgccatgaaa ttaaatatcc
    15421 gcgcgcaaac tgcacaaaac cagcacaata attctcccat cgttcttgtc catggtctgt
    15481 ttggtagcct cgacaacctt ggcgtactgg ctcgcgatct ggtaaacgat cacaatatca
    15541 tccaggttga tatgcgtaac cacggtcttt caccgagaga gccggtaatg aattacccgg
    15601 cgatggcgca ggatcttgtt gataccctgg atgcactgca gatcgacaaa gcaacattta
    15661 tcggtcactc catgggcggt aaagcggtaa tggcacttac tgcactggcc cccgatcgca
    15721 tcgataaact ggtggcgatc gatatcgcac cggtcgacta tcacgtacgc cgtcatgatg
    15781 agattttcgc agctatcaac gcggtcagtg aatcggacgc acaaactcgc cagcaagcag
    15841 cagcaataat gcgccagcat cttaatgaag aaggggtgat tcagtttctg ttgaaatctt
    15901 ttgttgacgg ggagtggcgc tttaacgtgc cggtattgtg ggatcagtat ccgcatattg
    15961 tgggttggga gaaaatcccg gcatgggatc accctgccct gtttatccct ggcggcaatt
    16021 ctccgtatgt tagcgagcag taccgtgatg atttactggc acaatttcca caggcacggg
    16081 cgcatgtgat tgcaggcgcg ggtcactggg tccatgctga aaaaccggat gcggtattac
    16141 gcgctatccg tcgctatctc aatgattaac gcaactgatt aaaaacccag cgtccgcgtc
    16201 gctttccgcg cgtgggcgtt ggcgtgactc atcgcgctga tgtattatgg cgcgctatcc
    16261 atccgggcag aggcctggct gattgtttcc cccgaagtca ccaagatcat ggccaaagaa
    16321 caaacggacc gtacgacatt agatctgttc gcgcacgagc gtcgaccggg acgaccgaaa
    16381 actaatccgc tttcgcgcga tgaacagctg cgtattaata aacgcaacca gctaaaacgc
    16441 gacaaagtac gtggccttaa gcgtgtcgaa ctgaagctga acgcggaagc tgtcgaggcg
    16501 ctgaacgagc tggcggaatc gcgtaatatg agccgtagcg aactgatcga agagatgtta
    16561 atgcagcaac tggcggcgct gcgtagtcag ggcatcgttt aaatttccac tttcatgtag
    16621 cacagtgtgc agtcctgctc gtttgctgat tccgcaagac tgcctgttct gctatgattg
    16681 cctttatccg tgggcaattt tccaccccca tttcaataag tttcaagagg ttatttcact
    16741 catggctatc actggcatct ttttcggcag cgacaccggt aataccgaaa atatcgcaaa
    16801 aatgattcaa aaacagcttg gtaaagacgt tgccgatgtc catgacattg caaaaagcag
    16861 caaagaagat ctggaagctt acgacattct gctgctgggc atcccaacct ggtattacgg
    16921 cgaagcgcag tgtgactggg atgacttctt cccgactctc gaagagattg atttcaatgg
    16981 taaactggtt gcgctgtttg gttgtggtga ccaggaagat tacgccgaat atttctgcga
    17041 cgcattgggc accatccgcg acatcattga accgcgcggt gcaaccatcg ttggtcactg
    17101 gccaactgcg ggctatcatt tcgaagcatc aaaaggtctg gcagatgacg accactttgt
    17161 cggtctggct atcgacgaag accgtcagcc ggaactgacc gctgaacgtg tagaaaaatg
    17221 ggttaaacag atttctgaag agttgcatct cgacgaaatt cttaatgcct gatgtgatgc
    17281 ggcgtagact catgtctacg ccgtattaat agataatgcc aatcaaaata attgctacaa
    17341 atttgtaact tttgctgttg tacctataca atgtcccagt gttcaagtgg ccttgccgtt
    17401 gtaaatgtaa gctgtgccac gtttttatta acaatatttg ccagggactt gtggttttca
    17461 tttaggcgtg gcaattctat aatgatacgc attatctcaa gagcaaattc tgtcacttct
    17521 tctaatgaag tgaaccgctt agtaacagga cagattccgc atgactgata acaataccgc
    17581 cctaaagaaa gctggcctga aagtaacgct tcctcgttta aaaatcctgg aagttcttca
    17641 ggagccggac aaccatcacg tcagtgcgga agatttatac aaacgtctga tcgatatggg
    17701 tgaagaaatt ggtctggcta cggtatatcg cgtactgaac cagtttgacg acgctggtat
    17761 cgtcacccgc cacaattttg aaggcggtaa atccgtattt gaactgacac agcaacatca
    17821 ccacgatcac ctgatttgcc tcgactgcgg caaggttatc gaatttagtg atgattccat
    17881 cgaagcgcgt cagcgtgaaa ttgccgcaaa acatggcatt cgcctgacta accacagtct
    17941 ctatctttac ggtcactgtg ccgaaggcga ttgccgcgaa gatgagcacg cgcacgaagg
    18001 caaataagcc agcctgaacg agaaaagcca acctgcgggt tggctttttt atacaaggga
    18061 aataagaaaa gcgagagtta cagctctcac ttatttgtta ttactgcgta tttcctgcca
    18121 gaccttatca caatcggctt tcaccgcctg atcatttccg gtttgcgttg cacgcagaca
    18181 ctgctgataa tccagtacgc gtacactttc ctggtcggca aactgctgat gttgtttctc
    18241 tttcttcagc acgtttaaca cgctctggca ggcttcaatt ttttctggcg aaccttgcgc
    18301 ggtgttgata caggcgctgt atgcctcttt cagacggctg tcttcctgtg gtgcggttga
    18361 ttgcgcacaa gctaccagcc ccgatgccat catggcgatg agaatcagtt ttttcattga
    18421 atgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg gtgcgattac
    18481 cataaatttc acgtcacgct catcctggaa gatgttaccg taaccgccgc cccagcttgg
    18541 gatgtcggag tggttgtcgt attcggtgaa gtgcagtttg aacatcgtgc ctttggcgcg
    18601 accgtcctga atggtgtaga ccgcatccag gctgtaggca gactcttcaa tagtccggtt
    18661 tttgtcgtag tacgcatccg gattgctctg ccaggtcgca ggtttagcat cccatgcgta
    18721 aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt catacatcgc
    18781 actgaagaag accgcttttt cgccgttagc gttgaagtcg gaacggttat cccaccagat
    18841 atccaggcgg ccgttggagg aagcgtaggt tggggtcata cgttgcagga agtatccctg
    18901 ctgactgtca gccttaatcc aggtgccttc gaggcgcaaa tccactacgt cagccgcccg
    18961 gtaaccaaag gtcaacgctt gcagccaggc ggtgccgtca taaagatcgt tgacgctgcg
    19021 atcgtcaact ttatcgcggg taccatagaa ctggtagctg gtggttaacg ggctaccggc
    19081 gatatcaaat ttgtagctgg ctttggcaaa atattgatcg atataccctt ccgcctgacc
    19141 aaatgccgct tccagtacga agttattttt gaagtcgtat ttcgcaccga tggagtgcag
    19201 ataatcaact ttggtggttt tatcgttctg ataaaactca tccatttcca gatgccacgg
    19261 cgctttgtat tcgttggtcc acatgtagga gaaactcaag gcaccagcat cgccgtaatc
    19321 aaaattcgcc ccggcttccg ccccctgata agtacccggc ataaagctcc agtgcggcgc
    19381 taacagcgtt tgaccggttg gctgaatgta acctgccctc gcccaaaccg gaccgtattt
    19441 aaatttggcc gcagctttat acaggcttat accgcttttg tcgccggacc agtcttcgtc
    19501 ataggcttta ttactttttg aaaacgcgat ttcgttcggg tgggagctgt cgccgttttc
    19561 cgccatttca atcgccgtaa acgcagcaat atcaaggccg aacatatcag cagcataacc
    19621 ggactgaaaa tcgaggttgg cattccaggt ggagtgagaa aggttggttt tgtatttgtc
    19681 gccgtcggta acatctttac ggtcgcgttc acgctgccag taatagatac cgccggttaa
    19741 ggttgaatcg tcgatgaatc cttcggcgcg agcctccggc attgccataa agcccgacat
    19801 tgctgtaaca ccggcaatag ccagcgccag cgtactacgt ttgccactaa acgtacgcat
    19861 gggttaatcc tctttgacgt ataaattgct gcaccaaagg tgaacagcga aaaaaaataa
    19921 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    19981 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    20041 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    20101 aacgtgaaat tccagcaaga tgaaagcggt tacattttat ttaattatgt tttgtgattt
    20161 taaatttatt ttatggataa attgcgaagc ggaattaatt cgcgaagaaa agcagaaaaa
    20221 acgccgcaga gcgcggcgtt tgggattgtt tgctatttct tgccggatgc ggcgtgaacg
    20281 ccttatccgg cctacaaaag cacgcaaatt caatatattg cagagatcat gtaggcctga
    20341 taagcgaagc gcatcaggca atttagcgtt tgaaatttat tgtctattta ttgccggatg
    20401 cgacgtaaat gcctcacccg gcttggtaga acttaaaatt actcgcctac tttcgcccag
    20461 gtatcacgca gcccaacggt gcggttaaat accggttttt cggccgtgga atggcggctg
    20521 tcgaggcaga agtaaccttc acgctcaaac tggaatgctt tacccgcaac cgcatctttc
    20581 agcgacggtt cagcaaagcc ctgtttgatc accagcgatt ccgggttaat caccgacagg
    20641 aaatcatccg cagcacctgg gttaggcacg ctgaacagac gatcatacaa acggatttca
    20701 accggcagcg catgtgccgc gctcacccag tgaataacac ctttgacttt acgaccatct
    20761 gccggatctt tgcttaaggt gtcggcgtca taagtacaga agatggtggt gatattacct
    20821 tcggcatctt tctccacgcg ttcagcctta atgacgtaag cattacgcag acgcacttct
    20881 ttacccagca ccagacgttt gtactgcttg ttagcttctt cgcggaaatc ggcgcgatcg
    20941 atccaaattt caccgctaaa cggcacctga cggctcccca tttccggttt gttcggatgg
    21001 ttcggcatgg tgaccatttc gccttcgccc tgatagtttt cgataaccag tttcaccgga
    21061 tcgataaccg ccattgcgcg cggcgcattt tcgttgagat cttcacggat gcaggattcc
    21121 agcgacgcca tctcaatggt gttgtcctgc ttggtcacgc cgatgcgttt gcagaactca
    21181 cgaatagaag ccgcagtgta accacgacga cgcagaccgg aaatggtcgg catacgcggg
    21241 tcatcccagc cttcaacgtg cttgtcggtc accagcaggt tcagcttacg cttggacatc
    21301 acagtgtatt ccagattcag gcgcgagaat tcgtactggc gcggatgaac aggaatggtg
    21361 atgttatcca gtacccagtc gtacagacga cggttgtcct ggaactcaag cgtacacaga
    21421 gagtgcgtaa taccttccag cgcatcgctg atgcaatggg tgaagtcgta catcgggtag
    21481 atgcaccact tgttgccagt ctggtggtgt tcagcaaatt taatgcggta cagcaccgga
    21541 tcgcgcatta cgataaatgg cgacgccatg tcgattttcg cacgcaggca ggctttacct
    21601 tcttcaaaac caccggcacg cattttttcg aacagcgcca agttctcttc aacgctgcgg
    21661 tcgcggtacg ggctgttttt acccggctgc gtcagggtac cgcggtattc gcggatctgt
    21721 tccggtgtca gttcatcaac gtacgccagg cctttattga tcagttcgat cgcataggcg
    21781 tggagctgat caaaataatc ggaggagtaa cggacgttac cagaccagtg aaaacctaac
    21841 cactctacgt cgtttttgat cgaatcgaca tactcgatat cttcttttac cgggttagtg
    21901 tcgtcgaaac gcaggttgca ctggccttta tagtcctggg cgatcccgaa gttcaggcag
    21961 atagatttcg catggccaat atgcagatag ccattcggct ccggcgggaa acgggtgtgt
    22021 actgtgatgt gcttaccact ggccagatct tcatcgatga tctgacggat aaagttagtc
    22081 gggcgggctt ctgcctcact catcgtggat tcctcaaagc gtaaacaacg tataacggcg
    22141 tatgatctta taagcgggac aggctgacaa ctgttagttt tttaaaattg ataatctaac
    22201 aaggattatg gggatgattg atgaaaaaaa aagcggcaga gaagatctcc accgcttgtc
    22261 attgttggat gcgacgctgc cgtgtcgcat caggcataaa gcagattact ttttgatttc
    22321 atacagcggt gtttgacccg ccacaacatg gccctgagct ttaatgagca agccactgaa
    22381 atcgtcgata ttgctgcaaa ccaccgggct aatcatcgag cgggcgttag cgttcaggta
    22441 atccagatcc atttccagaa tcggttgccc tgcgcttacc tgcgcaccct cttccaccag
    22501 acgtttaaag cctttacctt ccagcgctac ggtgtcgata cccatatgga cgacgatctc
    22561 cgcgcctttt tcggtttcca ggcagaacgc gtggttggtg ttgaagattt tcacgattgt
    22621 ccctgcggct ggtgatacga cgattttatc tgtcggtttc accgccacac cgtcacccac
    22681 cgctttgctg gcgaatgctt cgtcaggaac ctgatccagt gccacgacat caccggtaat
    22741 cggcgatacc agctccgcga tagataccgc gtttggtaca gcctgcggtt ttgctacagg
    22801 cgcggcagtt gccggagttg cttcagctga cgcagcggct accggaccac gggcaacgac
    22861 tttcttcatc gcatcgccga tggattctgc tttcgcgcca acaatcacct gaatagtctg
    22921 tttgttcagt ttcactacac cagaagcacc cagacgttta cacatcgtat cgttgacgcg
    22981 ggcagagtca gccacggtca ggcgcagacg ggtaatacag gcatcaatcg ctttcaggtt
    23041 gtcagtaccg ccaaccgcag caatatagtt ggttgccagt tgagtcagac cttcttcagt
    23101 attgctgttg gcttcttcag taacgatctc gtcttcttta tcttcacgac ccggcgtttt
    23161 caggttgaac atgcggataa ccaaactgaa caccacgaag tagatagcga agaagataac
    23221 gcccatcacc agcagcatcc agacgttctg gctggcggca ggcaggttat acatcaacgc
    23281 gtagtcgata gcacccgcag agaaagagaa gccggcatgg atacccagca gcgttgccac
    23341 aaacaggctg ataccggtca gcagtgcgtg caggaggtac agcagcggcg caaggaacat
    23401 gaacaggaat tccagcggct cagtcacacc ggtcaggaac gcagtaacag caacagaaag
    23461 cagcatcccg ccaaccatcg gacgacgctc tttcggtgct gcgaagtaca tcgccagcgc
    23521 cgcacccggc agaccgaaca tcatgatcgg gaagaagccg gacatgaaca tccccgcggt
    23581 gccgtcacca gcgtagaaac ggttgatgtc gccgtggaaa accgtacccg ccgcgttggt
    23641 gaattcacca atctggaacc aggcgatggt gttcagcacc tgatgcagac cggttgggat
    23701 cagcagacgg ttgatgaaac caaagatacc ggaacccagc gcgcccgcag aaacgatcca
    23761 ctcgccgcct gcatggatag cgtgctgtac cggcggccag acgtaaccaa aaatggccgc
    23821 cagcaccaga cagaagaagc cggtggcgat cggcacaaag cgtttgccgc cgaagaagct
    23881 caggaagtcc ggcagtttaa tatcggacca acggttatag gctgcgccac caaccagacc
    23941 ggtaatgata cccgccagta cacccatgtt aatttctggg ttgatggtca ccatcgcttt
    24001 ggttaacaca aagtaaccta ccgcacccgc cagcgccgcc gcacctgcgc tgtctttcga
    24061 ccagctggat gccacaccga tggcgaagat taatgcgagg ttatcaaaaa ttgcaccgcc
    24121 cgcctgggca ataaacgcaa cgttaagtaa atctggctga ccgaatcgca gcaacagcgc
    24181 cgccaccggc agcaccgcga tagggagctg taacgcccta ccgagtcgct ggaaaaaacc
    24241 taaaatattc atcttattcc ccctacgaga accctatttg gctcgtttaa agccgtattt
    24301 ttattttgct gcaaatttta ctgccgatgt tctgtaatca gattgttaga tcatctgcta
    24361 cagagtgtgt gaaaatttaa ttcgcatcgc aaattaaacg cgtgtctttt gtgagttttg
    24421 tcaccaaata tcgttatcat cactcccttt tactggctaa accagaaaac ttattttatc
    24481 attcaaaaaa tcaagtcgga ttgacgcctg tctgcgcaaa tccaggttac gcttaaagat
    24541 gtctaatccg ccaacggctt acattttact tattgaggtg aataatgaga ctgatccccc
    24601 tgactaccgc tgaacaggtc ggcaaatggg ctgctcgcca tatcgtcaat cgtatcaatg
    24661 cgttcaaacc aactgccgat cgtccgtttg tactgggcct gccgactggc ggcacgccga
    24721 tgaccaccta taaagcgtta gtcgaaatgc ataaagcagg ccaggtcagc tttaagcacg
    24781 ttgtcacctt caacatggac gaatatgtcg gtctgccgaa agagcatccg gaaagctact
    24841 acagctttat gcaccgtaat ttcttcgatc acgttgatat tccagcagaa aacatcaacc
    24901 ttctcaacgg caatgccccg gatatcgatg ccgagtgccg ccagtatgaa gaaaaaatcc
    24961 gttcttacgg aaaaattcat ctgtttatgg gcggtgtagg taacgacggt catattgcat
    25021 ttaacgaacc ggcgtcttct ctggcttctc gtactcgtat caaaaccctg actcatgaca
    25081 ctcgcgtcgc gaactctcgt ttctttgata acgatgtcaa tcaggtgcca aaatatgccc
    25141 tgactgtcgg tgttggtaca ctgctggatg ccgaagaagt gatgattatg gtgctgggta
    25201 gccagaaagc actggcgctg caggccgccg ttgaaggttg cgtgaaccat atgtggacca
    25261 tcagctgtct gcaactgcat ccgaaagcga tcatggtgtg cgatgaacct tccaccatgg
    25321 agctgaaagt taagacttta agatatttca atgaattaga agcagaaaat atcaaaggtc
    25381 tgtaattgtt atccctgccc tggttccttg ctcagggcaa tattttttaa aatcgggggg
    25441 cagaatgtat gcattaaccc agggccggat ctttaccggc cacgaatttc ttgatgacca
    25501 cgcggttgtt atcgctgatg gcctgattaa aagcgtctgt ccggtagcgg aactgccgcc
    25561 agagatcgaa caacgttcac tgaacggggc cattctctcc cccggtttta tcgatgtgca
    25621 gttaaacggc tgcggcggcg tacagtttaa cgacaccgct gaagcggtca gcgtggaaac
    25681 gctggaaatc atgcagaaag ccaatgagaa atcaggctgt actaactatc tgccgacgct
    25741 tatcaccacc agcgatgagc tgatgaaaca gggcgtgcgc gttatgcgtg agtacctggc
    25801 aaaacatccg aatcaggcgt taggtctgca tctggaaggt ccgtggctga atctggtaaa
    25861 aaaaggcacc cataatccga attttgtgcg taagcctgat gccgcgctgg tcgatttcct
    25921 gtgtgaaaac gccgacgtca ttaccaaagt gaccctggca ccggaaatgg ttcctgcgga
    25981 agtcatcagc aaactggcaa atgccgggat tgtggtttct gccggtcact ccaacgcgac
    26041 gttgaaagaa gcaaaagccg gtttccgcgc ggggattacc tttgccaccc atctgtacaa
    26101 cgcgatgccg tatattaccg gtcgtgaacc gggcctggcg ggcgcgatcc tcgacgaagc
    26161 tgacatttat tgcggtatta tcgctgatgg cctgcatgtt gattacgcca acattcgtaa
    26221 cgctaaacgc cttaaaggcg acaaactgtg tctggttacc gacgccaccg cgccagcagg
    26281 tgccaacatt gaacagttca tttttgcggg taaaacaata tactaccgta acggactttg
    26341 tgtggatgag aacggtacgt taagcggttc atccttaacc atgattgaag gcgtgcgtaa
    26401 tctggtcgaa cattgcggta tcgcactgga tgaagtgctg cgtatggcga cgctctatcc
    26461 ggcgcgtgcg attgacgttg aaaaacgtct cggcacgctc gccgcaggta aagtagccaa
    26521 cctgaccgca ttcacacctg attttaaaat caccaagacc atcgttaacg gtaacgaggt
    26581 cgtaactcaa taagagaaag tatgacacca ggcggacaag ctcagatagg taatgttgat
    26641 ctcgtaaaac agcttaacag cgcggcggtt tatcgcctga ttgaccagta cgggccaatc
    26701 tcgcggattc agattgccga gcaaagccag cttgcccccg ccagcgtaac caaaattacg
    26761 cgtcagctta tcgaacgcgg gctgatcaaa gaagttgatc agcaggcctc caccgggggc
    26821 cgccgcgcta tctccatcgt caccgaaacc cgcaatttcc acgcaatcgg cgtacggctt
    26881 ggtcgtcatg acgccaccat cactctgttt gatctcagca gcaaagtgct ggcagaagaa
    26941 cattacccgc tgccggaacg tacccagcag acgctggaac atgccctgct gaatgccatt
    27001 gctcagttta ttgatagcta ccagcgcaag ctgcgcgagc taatcgccat ttcggtgatc
    27061 ctgccagggc ttgttgaccc ggacagcggc aaaattcatt acatgccgca tattcaggta
    27121 gaaaactggg ggctggtaga agcactggaa gaacgtttta aagtgacctg tttcgttggt
    27181 cacgatatcc gtagtctggc gctggcggag cactacttcg gtgcaagtca ggattgcgaa
    27241 gactccattc tggtgcgtgt ccatcgcgga accggggccg ggattatctc taacgggcgc
    27301 atttttattg gccgcaacgg taacgtcggt gaaattggcc atattcaggt cgaaccgctg
    27361 ggtgaacgct gccactgcgg caactttggc tgcctggaaa ctatcgccgc caacgctgcc
    27421 attgaacaac gggtgttgaa tctgttaaag cagggctacc agagccgtgt gccactggac
    27481 gactgcacca tcaaaactat ctgcaaagcc gcgaacaaag gcgatagcct ggcctcggaa
    27541 gtgattgagt atgtcggtcg tcatctgggt aaaaccatcg ccattgctat caacttattt
    27601 aatccgcaaa aaattgttat tgctggtgaa atcaccgaag ccgataaagt gctgctccct
    27661 gctattgaaa gctgcattaa tacccaagcg ctgaaggcgt ttcgtactaa tctgccggtg
    27721 gtacgttctg agctggatca ccgctcggca atcggcgctt ttgcgctggt aaaacgcgcc
    27781 atgctcaacg gtattttgct ccagcatttg ctggaaaatt aatgtgcttt tatagtggcg
    27841 cttattgttg tcaatattct gggtagtcca tgaccattaa aaatgtaatt tgcgatatcg
    27901 acggcgtgct gatgcacgat aacgtcgccg taccgggtgc agcggaattt ttgcacggga
    27961 ttatggataa aggcctgccg ctggtgttgc tgaccaacta tccttcgcag actgggcaag
    28021 atctggcgaa ccgctttgcc actgccggtg tcgatgtgcc ggatagcgtg ttttatacct
    28081 ctgcgatggc gaccgccgat ttcctgcgtc gctaggaagg caagaaagcg tatgtggtgg
    28141 gcgaaggcgc actgattcat gaactgtaca aagccggttt cactattacc gatgtgaacc
    28201 ctgatttcgt gattgttggc gaaacacgtt cctacaactg ggacatgatt cataaagcag
    28261 cctatttcgt cgctaacggt gcacgcttta tcgccaccaa cccggacacc cacgggcgcg
    28321 gtttttatcc cgcttgtggc gcgttgtgtg cagggattga gaaaatctcc gggcgcaaac
    28381 cgttctatgt tggtaagccc agcccgtgga tcatccgcgc ggcattaaac aaaatgcagg
    28441 cgcattcgga agaaacagtg attgtcggcg ataacctgcg taccgatatt ctggcgggct
    28501 tccaggccgg tctggagacg attctggtgc tttctggtgt ttcatcgctc gacgatatcg
    28561 acagtatgcc gttccgccca agctggattt acccgtcagt cgctgaaatc gacgttatct
    28621 gaataccacc cacgtctgcg gacgtggttg gctctgatga caaacgccaa actgcctgat
    28681 gcgctacgct tatcaggcct acgcagctcc tgcaatatat tgaattttca tgcttttgta
    28741 ggccggataa ggcgttcacg ccgcatccgg catgaacaaa tcgcacttta tcagcaattt
    28801 ggcccccgta aaatataacc attcaataaa acccgctaaa aattatcgat tcatcaataa
    28861 atacatcctc acaccagacc ctttttcacc aatcgacaat caccattacg ttttttattt
    28921 ttgcactgcc aaatagcttg cgcacagtgc tgctttgcgg cattttttta aacaagcaaa
    28981 cacaacaagc aacaaatacc aggttaacgg agaaggttat gtgttcaatt tttggcgtat
    29041 tcgatatcaa aacagacgca gttgagctgc gtaagaaagc actcgagctg tcacgcctga
    29101 tgcgtcatcg tggcccggac tggtccggta tttatgccag cgataacgcc attctcgctc
    29161 acgaacgtct gtcaattgtt gacgttaacg cgggggcgca acctctctac aaccaacaaa
    29221 aaaaccacgt actggcggta aacggtgaaa tctacaacca ccaggcattg cgcgctgaat
    29281 atggcgatcg ttatcagttc cagacagggt ctgactgtga agtgatcctc gcgctgtatc
    29341 aggaaaaagg gccggaattt ctcgacgact tgcagggcat gtttgccttt gccctgtacg
    29401 acagcgaaaa agatgcctac ctgattggtc gcgaccatct ggggatcatc ccactgtata
    29461 tggggtatga cgaacacggt cagctgtatg tggcctcaga aatgaaatcg ctggtgccag
    29521 tttgccgcac gattaaagag ttcccggcgg ggagctattt gtggagtcag gacggcgaaa
    29581 tccgttctta ttatcatcgc gactggttcg actttgatgc agtgaaagat aacgtgaccg
    29641 acaaaaacga gctgcgtcag gcactggaag attccgttaa aagccatctg atgtctgatg
    29701 tgccttacgg tgtgctactt tctggtggtc tggattcctc aattatttcc gctatcacca
    29761 agaaatacgc agcccgtcgc gtggaagatc aggaacgctc tgaagcctgg tggccgcagt
    29821 tacactcctt tgctgtaggt ctgccgggtt caccggatct taaagcagcc caggaagtgg
    29881 caaaccatct gggcacggtg catcacgaaa ttcacttcac tgtacaggaa ggtctggatg
    29941 ccatccgcga cgtgatttac cacatcgaaa cttatgatgt gaccactatt cgcgcttcaa
    30001 caccgatgta tttaatgtcg cgtaagatca aggcgatggg cattaaaatg gtgctgtccg
    30061 gtgaaggttc tgatgaagtg tttggcggtt atctttactt ccataaagcg cccaacgtta
    30121 aagaactgca tgaagagacg gtgcgtaaac tgctggcctt gcatatgtat gactgcgcgc
    30181 gcgccaacaa agcgatgtca gcctggggcg tggaagcacg cgttccgttc ctcgacaaaa
    30241 aattccttga tgtggcgatg cgcattaacc cgcaggataa aatgtgcggt aacggcaaaa
    30301 tggaaaaaca catcctgcgt gaatgttttg agtcatacct gcccgcaagc gtggcctggc
    30361 ggcagaaaga gcagttctcc gatggcgtcg gttacagttg gatcgacacc ctgaaagaag
    30421 tggcggcgca gcaggtttct gatcagcaac tggaaactgc ccgcttccgc ttcccgtaca
    30481 acacgccgac ctcaaaagaa gcgtatctgt accgcgagat ctttgaagaa ctgttcccac
    30541 ttccgagtgc cgctgagtgc gtgccgggcg gtccttccgt cgcgtgttct tcagctaaag
    30601 cgatcgaatg ggatgaagcg ttcaaaaaaa tggacgatcc gtctggtcgt gcggttggcg
    30661 ttcaccagtc ggcgtataag taagaaatgc ggtactgccc cggagaaatc cggggctgtt
    30721 ttagttattg cgctgcttgt tgattctgat atgcagtaag gaaatgttcc gggtttgcca
    30781 catcactgtt tgacaactca tcactgtagg ccagtatttc atagattaat ttcccaccac
    30841 tattgttgaa ttccgaattt catcctgtac gacaaaagaa tggctgtagg gtggggaaaa
    30901 acacaaagta aacaataatt gacgaatata gcgccacgct gttcgcaacc taaccaaaca
    30961 gtcactttcg agcaattttc cttgaaaaag aggttgacgc tgcaaggctc tatacgcata
    31021 atgcgccccg caacgccgat aaggtatcgc gaaaaaaaga tggctacgta gctcagttgg
    31081 ttagagcaca tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccatc
    31141 ttttttgcgg gagtggcgaa attggtagac gcaccagatt taggttctgg cgccgcaagg
    31201 tgtgcgagtt caagtctcgc ctcccgcacc attcaccaga aagcgttgta cggatggggt
    31261 atcgccaagc ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta
    31321 ccccagccat cttcttcgag taagcggttc accgcccggt tattggggta tcgccaagcg
    31381 gtaaggcacc ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc
    31441 gaagaaataa tctggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    31501 tcacaggttc gaatcccgtc gtagccacca aattctgaat gtgtcgaata cgttcggcaa
    31561 attcaaaaac caatttgttg gggtatcgcc aagcggtaag gcaccggatt ctgattccgg
    31621 cattccgagg ttcgaatcct cgtaccccag ccaatttatt caagacgctt acatcgtaag
    31681 tgcacccagt tggggtatcg ccaagcggta aggcaccgga ttctgattcc ggcattccga
    31741 ggttcgaatc ctcgtacccc agccacatta aaaaagctcg cttcggcgag ctttttgctt
    31801 ttctgcgttc attcaatgtc gaatgcgatg ttgacacgtc ttatccttca atgtcggatg
    31861 cgacgctgcc gcgtcttatc cgacctacgg ctggcacgca tccggcaatg ttgtaaggct
    31921 acaaccctaa cgcatatttc agcgcctgac gtttcaacac gccagcacgc tctgccgcca
    31981 ttaacccgag attacgcaca aaacgcagcg gcggcagatt attactgaat ccggcataaa
    32041 acagatccat accactttgc ataatgaagt tatccgccat gcgccgcatc tggtaacgct
    32101 tgaggacagg ataactggcc cacgcttcac cgtagctgcg ggcattcacc agaacatcaa
    32161 tcagggcatc gacatcacga taaccaagat tcaccccctg ccccgccagc ggatggatgg
    32221 tatgcgcggc atcgcccacc agcgcaagcc ccggctgcac atactgcaac gcatgtcggc
    32281 gcgtcagcgg aaacgcacca gcggcaagcg gtgtaacgta acccagacgc gacgggaaat
    32341 gcttcgcgat ttccgcctgg agttgcgcca tattcatatt ctgcaactgg cgaatacgcg
    32401 tcggagagtc ataccacacc agcgatgccc agttatcaaa caacggcaga aacgcacgcg
    32461 gtccgtccgg ggtaaattgc tgccaggtgc tgtcgccggg atcgttctca cactgtacgc
    32521 taatcaacat gcacgactgc gcatactgcc aggcatgaac gccaattccc gccatctgcc
    32581 gcacctgaga atttgcgccg tcggcaccaa tcaccagttt cgcgcggatc gtttcaccgc
    32641 ccttcagttc cagctcctga agatcattat ctcgatgtaa tgcaatcagc gaggttggca
    32701 cacgtaacgt tactttcgga tgcgcttcca gcgcctgcca caacgcctgt tgcaggactg
    32761 tgttttccac catgtagcca agcaacggca actttaattc ggctgcgtca aataccacat
    32821 gtgccgtttc ccactcccag gtttccagtc tgcggtaagg atggcaacgc atagcctgta
    32881 ctgcatccca gacccctaac cctttaagca atgataccga agccgcgctg atcgccgaga
    32941 tccgcacgtc cggttggctg tcagcgacaa acggtgcagg ttctgcgtgc tcgatcaccg
    33001 ttaccgaaaa tccgtgctgt gccagcccca gcgccagtgc gccgccgacc attcctccgc
    33061 cgacaatggc aatttccgtt ggttgatttg tcatggtcaa tcatcctgtt agcaatatgc
    33121 tttaagttta caggattttt acccccaagg gctgataacg ctcatactgg tcacaacggc
    33181 agcaaagcat tacactatgc gccctgcatt cctggctact atttcgcaag agcaagtcga
    33241 tgaccaaaaa actccatatt aaaacctggg gctgtcagat gaacgagtac gattcatcga
    33301 agatggccga tctgctggat gccacccacg gctatcaact gaccgacgtg gcggaagaag
    33361 cggatgtgct actgctgaac acctgctcaa tccgcgagaa ggctcaggaa aaagtcttcc
    33421 atcagttggg tcgctggaaa ctgttaaaag agaagaatcc agacctgatt atcggcgtcg
    33481 gtggctgcgt ggcatcgcaa gaaggcgagc acattcgcca gcgcgcccac tatgtcgata
    33541 ttatttttgg gccgcaaacg ctgcaccgtc tgccggagat gatcaactcc gtgcgcggcg
    33601 accgcagccc ggttgtagat atcagcttcc cggaaatcga gaagtttgac cgtctgccgg
    33661 aaccgcgcgc cgaagggccg accgcgtttg tctccatcat ggaaggctgc aataaatatt
    33721 gcacctactg cgtggtgcct tacacccgtg gtgaagaggt aagccgtccg tctgacgata
    33781 ttctgtttga gattgcccag cttgcggctc agggcgtgcg tgaagtcaac ctgctcggtc
    33841 agaatgtgaa tgcctggcgt ggtgagaact acgacggcac caccggatcg tttgccgatc
    33901 tgttgcgtct ggttgctgcg atcgacggga tcgatcgtat tcgctttacc accagccatc
    33961 cgatcgaatt caccgacgat atcatcgaag tgtaccgcga cacgcctgag ctggtgagct
    34021 tcctgcatct gccggtacag agcggttccg atcgcattct gaacctgatg ggacgtaccc
    34081 atacggcgct ggagtacaaa gcgatcatcc gtaaactgcg tgcggcgcgt ccggatattc
    34141 agatcagttc tgacttcatt gttggcttcc ctggcgaaac caccgaagac ttcgagaaaa
    34201 cgatgaagct gattgccgac gtcaatttcg acatgagcta cagctttatc ttctctgcac
    34261 gtccaggaac gcctgccgcc gatatggttg atgatgttcc ggaagaagag aagaagcagc
    34321 gtctgtatat tctgcaagaa cgtatcaatc agcaagcgat ggcatggagc cgtcggatgc
    34381 tcggcaccac ccagcgtatt ctggtagaag ggacttcgcg taagagcatc atggagcttt
    34441 ccgggcgtac cgaaaataac cgcgtggtca acttcgaggg cacaccggat atgatcggta
    34501 aattcgtcga tgtagaaatt accgacgtct acccgaactc tctgcgcggt aaagtggtgc
    34561 gaactgaaga tgaaatgggt ctgcgcatgg cagaaacacc ggagtcggtg attgcccgca
    34621 cccgcaaaga aaacgacctt ggcgtgggtt attatcagcc gtaattctca ggcctgccgt
    34681 tccggtgggc cttgtttttt ctctctttat ccccatcttt atcgcaatgc ttgcgccttt
    34741 ttcctgtggc gtgaataatt tccttatagg ccggtgaatg ttcagcttcc ggcggcatac
    34801 agataagagg aacggtttga acatagacac tcgcgaaatc accctggagc cagcagacaa
    34861 tgcgcgtctg ttgagcctgt gcggcccgtt tgatgacaac atcaagcagc tcgaacgccg
    34921 tctcggcatc gagatcaatc gccgcgataa ccactttaaa ctgaccggcc gtccgatttg
    34981 cgtcaccgct gcggcagaca ttctgcgtag cctgtatgtc gatactgccc cgatgcgcgg
    35041 tcagattcag gatatcgaac cggaacagat ccaccttgcg attaaagaag cgcgggtact
    35101 ggagcaaagc gcggagagcg tgccggagta cggcaaagcg gtcaatatca aaactaaacg
    35161 cggcgtaatt aagccgcgca cgccaaacca ggcgcagtac atcgccaata ttctcgacca
    35221 tgacattacc ttcggcgttg gcccggcggg tacgggtaaa acctacctgg cagtggctgc
    35281 ggcagttgat gccctggagc gtcaggaaat tcgccgtatt ctgctgactc gtccggcggt
    35341 cgaagccggt gagaaactgg gcttcctgcc tggcgattta agccagaaag tagacccgta
    35401 tctgcgccca ctgtacgacg cgctgtttga aatgctgggc tttgagaaag tcgagaaact
    35461 gattgagcgc aacgttattg aagtcgcgcc gctggcctat atgcgtggtc gtacgctgaa
    35521 cgacgcgttt atcattctcg atgagagcca gaacactacc atcgaacaga tgaagatgtt
    35581 cctgacccgt atcggtttta actcaaaagc ggttatcacc ggcgacgtca cacagatcga
    35641 cctgccgcgt aatactaaat caggcttacg tcatgccatt gaagtgctgg ccgatgtcga
    35701 agagatcagc tttaacttct tccacagcga agacgtggtt cgtcacccgg tggtggcgcg
    35761 tatcgttaac gcttatgaag cctgggaaga agcagaacaa aaacgtaaag cggcgctggc
    35821 agcagaacgc aagcgcgaag aacaggaaca aaaatgagtc aggtgatcct cgatttacaa
    35881 ctggcatgtg aagataattc cgggttaccg gaagagagcc agtttcaaac atggctgaat
    35941 gcggtgatcc cgcagtttca ggaagagtcg gaagtgacta ttcgcgtggt cgataccgcc
    36001 gaaagccaca gtctgaatct gacctatcgc ggtaaggata agccgaccaa cgtgctctcc
    36061 ttcccgtttg aagtgccgcc tggcatggag atgtcgctac tgggcgatct ggttatctgc
    36121 cgtcaggtgg ttgagaagga agctcaggag caaggcaaac cactggaggc gcactgggcg
    36181 catatggtgg tgcacggcag tctgcatttg ttaggttacg atcacattga agatgacgaa
    36241 gcagaagaaa tggaagccct cgaaacagag attatgcttg ctctgggcta tgaggatccg
    36301 tacattgccg agaaagaata atttgccagg catttgactg gcagacgtcc ccgccgttgg
    36361 tgattaacga cggcgttttg attaactaac atgagaaccc atacgacgcc atgagcgacg
    36421 acaattcaca cagtagtgac acgataagca acaagaaggg atttttctcc ctgttactca
    36481 gccaactttt ccacggtgaa ccgaaaaacc gtgacgaact gctggcgctg atccgtgatt
    36541 ccgggcagaa cgaccttatc gacgaagata cgcgcgatat gctcgaaggg gtgatggaca
    36601 tcgcagacca acgcgttcgc gacatcatga tcccccgctc ccagatgatt accctgaaac
    36661 gcaaccagac gctggacgaa tgccttgatg tcatcatcga gtccgcccac tcacgtttcc
    36721 cggtgattag cgaagacaaa gatcacattg aagggattct gatggcgaaa gacttgctgc
    36781 cgtttatgcg cagcgatgct gaagccttca gcatggacaa agtgttacgt caggcggttg
    36841 tcgttcctga aagtaagcgc gtagaccgga tgctgaaaga gtttcgctct cagcgttacc
    36901 acatggcgat cgttattgac gaattcggtg gggtttccgg tctggtgacc attgaagaca
    36961 tcctggaact gattgttggt gagattgaag acgagtatga cgaagaagat gatatcgact
    37021 tccgtcagct gagtcgtcat acctggaccg tgcgcgcact ggcttccatt gaagacttca
    37081 acgaagcgtt cggcacccac tttagcgatg aagaagtcga cactatcggt ggtctggtga
    37141 tgcaggcgtt tgggcatctt ccggcgcgtg gcgaaactat cgacatcgac ggttaccagt
    37201 tcaaagtggc gatggccgat agtcggcgta ttattcaggt tcatgtcaaa atcccggatg
    37261 actcacccca gccaaagctg gatgaataaa accgaaactg gatagataac tacatggatt
    37321 ttgcctcatt aattgaacgc cagcgcattc gcctgctgct ggcgttatta ttcggtgcct
    37381 gcggaacgct ggccttctct ccttacgacg tctggcctgc ggcgattatt tcgctgatgg
    37441 ggcttcaggc gttgaccttt aaccgtcgcc cactccagtc tgccgctatt ggcttttgct
    37501 ggggatttgg cctctttggc agcggtatta actgggtcta tgtcagcatc gcgacctttg
    37561 gcgggatgcc tggcccggtt aacatcttcc tggtggtgct gctggcggcg tatttgtcgc
    37621 tgtataccgg actgtttgct ggcgtgctgt cgcgtctgtg gccgaaaacc acctggctgc
    37681 gcgtggcgat tgccacccct gctctctggc aagtgaccga gtttctgcgc ggttgggtgc
    37741 tgactggctt cccgtggtta cagttcggct atagccagat tgatggcccg ttaaaagggc
    37801 tggcaccgct aatgggcgtg gaagccatta acttcctgct gatgatggtt agtggcctgc
    37861 tggcactggc gttggtcaaa cgcaactggc gtccgctggt ggtggccgtc gtgctgtttg
    37921 ccctaccctt cccgctgcgt tacatccaat ggtttacccc gcaaccggag aaaaccattc
    37981 aggtttcgat ggttcagggc gatattccgc aatcgctgaa atgggacgaa ggccagcttc
    38041 ttaatacgct aaagatttac tacaacgcaa cggcaccgct gatgggcaaa tcatcgttga
    38101 ttatctggcc ggagtcggcg ataaccgatc tggaaattaa tcagcaaccg ttcctcaaag
    38161 cactggacgg tgagttgcgt gataaaggta gctcgctggt aaccgggatt gtcgacgcgc
    38221 gtctcaataa gcagaaccgc tacgatacct acaacaccat catcacgctg ggtaaaggtg
    38281 caccgtacag ctacgaatca gccgatcgct ataacaaaaa ccatctggtg ccgtttggcg
    38341 agtttgtccc gctggagtcg attctgcgtc cgttagcacc gttctttgat ctgccgatgt
    38401 cgtcgttcag ccgtgggcca tatatccagc cgccgctgtc ggcaaatggt attgagctta
    38461 ctgcggctat ttgctacgag attattctcg ccgagcaagt gcgcgataac ttccgcccgg
    38521 ataccgacta cctgctgact atctccaacg atgcgtggtt tggtaaatct attggtccat
    38581 ggcaacactt ccagatggcg cgaatgcgtg cgctggagct ggcgcgccca ctgttgcgca
    38641 gcaccaacaa cggcattacg gcggtgattg gcccgcaggg tgagattcag gcgatgatcc
    38701 cgcagttcgc ccgcgaggtg ttaaccacta acgtgacgcc gaccaccgga ctcacaccat
    38761 acgcacgtac cggcaactgg ccgctgtggg tgctgacggc actgtttggt tttgctgctg
    38821 tgttgatgag tctgcgtcag cgacgtaaat aatcccttct tatcgtgccg gaatacatcc
    38881 catatttcgg cacgctcacc ggatgcggca tcagtgcatt atccggataa caatattctc
    38941 ctatccaact ttggcacatc tattgctttg ttatacaagg caaagcttga accagcatca
    39001 gtacaacccg gttgcaccac agcaatccac cggtagcacc aaaagagtgc attttaagaa
    39061 ttttgcttca gaatggtgcg gtgcacctcg caaaaacaaa caataaaaca tcaatatctt
    39121 tacatttagc caaactaaat gttacctaac aggcacaaca ctgcacaata aagttgcaga
    39181 cgataacaac acaaacactc acaacgggta tccatgcgtt cttaacgcag aagataaagg
    39241 tgttggatat gcaattacgt aaacctgcca cagcaatcct cgccctggcg ctttccgcag
    39301 gactggcaca ggcagatgac gccgccccgg cagcaggcag tacgctggac aaaatcgcca
    39361 aaaacggtgt gattgtcgtc ggtcaccgtg aatcttcagt acctttctct tattacgaca
    39421 atcagcaaaa agtggtgggt tactcgcagg attactccaa cgccattgtt gaagcagtga
    39481 aaaagaaact caacaaaccg gacttgcagg taaaactgat tccgattacc tcacaaaatc
    39541 gtattccact gctgcaaaac ggcactttcg attttgaatg tggttctacc accaacaacg
    39601 tcgaacgcca aaaacaggcg gctttctctg acactatttt cgtggtcggt acgcgcctgt
    39661 tgaccaaaaa gggcggcgat atcaaagatt ttgccgacct gaaaggcaaa gccgtagtcg
    39721 tcacttccgg cactacctct gaagttttgc tcaacaaact gaatgaagag caaaaaatga
    39781 atatgcgcat catcagcgcc aaagatcacg gtgactcttt ccgcaccctg gaaagcggtc
    39841 gtgccgttgc ctttatgatg gatgacgctc tgctggccgg tgaacgtgcg aaagcgaaga
    39901 aaccagacaa ctgggatatc gtcggcaagc cgcagtctca ggaagcctac ggttgcatgt
    39961 tgcgtaaaga cgatccgcag ttcaaaaagc tgatggatga cactatcgct caggtgcaga
    40021 cctccggtga agcggaaaaa tggtttgata aatggttcaa aaatccaatt ccgccgaaaa
    40081 acctgaacat gaatttcgaa ctgtcagacg aaatgaaagc actgttcaaa gaaccgaatg
    40141 acaaggcact gaactaatta caagaaccag gggcggaaat tccagccctc tcgattgtta
    40201 cgtagcacgg acagactata cgcctgatgg tcgttcccca tcgggcctga aaaccgcaat
    40261 acgctgggta acaatcttcg agggtagcag ttaacgctgc taccctcttt tttctggagt
    40321 agatttatgt ctatagactg gaactggggt atttttttac aacaagcccc gttcggcaat
    40381 accacctatc ttggttgggt ctggagtggt tttcaggtga cgatcgcttt gtcgatctgc
    40441 gcctggatta tcgctttcct cgtcggttca tttttcggca ttttacgtac cgttccaaac
    40501 cgttttctct ccggtctggg tacgttgtat gtcgaactgt tccgcaacgt gccgttgatt
    40561 gtgcaattct ttacctggta tctggtgatc ccggagctgc tgccggagaa aatcggcatg
    40621 tggtttaagg ccgagctgga tcccaatatt cagtttttcc tttcatccat gctctgcctg
    40681 gggctgttta ccgccgcccg tgtttgcgaa caggtacgcg ccgctattca gtcgctaccg
    40741 cgtgggcaga agaatgccgc gctggcgatg ggcctgacgc taccgcagac ttatcgatac
    40801 gtactgctgc ctaatgctta tcgcgttatc gtcccgccga tgacttccga gatgatgaac
    40861 ctggtgaaaa actccgccat cgcctcgacc atcggtctgg tggatatggc ggcgcaggcg
    40921 ggtaaattgc tcgactactc ggctcacgcc tgggagtcgt ttaccgccat cacgcttgcg
    40981 tatgtgttga ttaacgcttt tatcatgctg gtgatgacgc tggttgaacg taaagttcgc
    41041 ctgcccggca acatgggggg caaataatgt acgagtttga ctggagttcc attgttcctt
    41101 ccctgccata tctgctcgac gggctggtga tcaccctgaa aatcaccgtc acggcagtcg
    41161 tgattggtat tttgtggggt accatgctgg cggtgatgcg cttgtccagc ttcgcgcctg
    41221 tttcgtggtt tgccaaagcc tacgttaacg tattccgctc gattccttta gtcatggttt
    41281 tgctgtggtt ttacttgatt gtgccgggat ttctgcaaaa tgtgctggga ttatcgccaa
    41341 aaaatgatat tcgtttaatt tcggcgatgg tggcgttttc gatgtttgaa gcggcctact
    41401 attcagagat tatccgtgcc ggtattcaaa gtatttcccg tggtcagtcg agcgccgcgc
    41461 tggcgttggg gatgactcac tggcagtcga tgaaactgat tattctgccg caggctttcc
    41521 gcgcgatggt gccgctgctg ctcactcagg gcatcgtact gttccaggat acctcgctgg
    41581 tgtatgtgtt atctctggcc gatttcttcc gtaccgcctc aaccattggt gagcgtgatg
    41641 gtacgcaggt cgagatgatc ctgtttgccg gatttgttta tttcgttatt agccttagcg
    41701 cgtcgttgtt ggtcagctac ttgaaaagaa ggacagcata atgattaccc tgaaaaatgt
    41761 ttcaaaatgg tatggtcact ttcaggtgct gaccgactgc tcaaccgaag tgaaaaaagg
    41821 cgaagtggtg gtggtttgcg gcccgtctgg ttccggcaaa tcaacgctga ttaaaaccgt
    41881 caacggcctc gaaccagtgc agcaaggtga aatcaccgtc gatggtatcg tggttaacga
    41941 caagaaaacc gatctggcaa agctgcgctc ccgcgtcggg atggtattcc agcatttcga
    42001 gctgttccct catctgtcga ttatcgaaaa cctgaccctg gcgcaggtga aagtgcttaa
    42061 acgcgataaa gcgcccgctc gtgaaaaagc attgaaattg ctggagcgcg tgggactttc
    42121 cgcacatgcc aataaatttc cggcgcagct ttccggcggt cagcagcagc gcgtggcaat
    42181 cgctcgcgca ttgtgtatgg ctcctattgc gatgctgttc gacgaaccga catcggcgct
    42241 ggatccggag atgatcaacg aagtgctgga cgtgatggtg gaactggcga acgaaggaat
    42301 gaccatgatg gtggtgaccc acgaaatggg ctttgcccgc aaagtggcga atcgggtgat
    42361 ctttatggac gagggtaaaa ttgtcgaaga ctcgccgaaa gacgctttct tcgatgatcc
    42421 gaaatcggac cgcgcaaaag acttcctcgc gaaaatcctg cattaatcac tctggcgcac
    42481 atacccaatt gcgcgccatc tcacgcttta tcgtttcgcc cctcgtatct gttacatcct
    42541 ggcgttaccc ttgtcgcaaa gaagcacaca acaaggagca acaatggcac tgccaattct
    42601 gttagattgc gacccaggtc atgatgacgc tatcgcaata gttctcgcac tcgcctcacc
    42661 agagcttgat gtcaaagcaa ttacgtcttc cgccggaaac cagacaccag aaaaaacctt
    42721 acgcaatgtt ctgcgtatgc tgaccttgct taatcgcacc gatattccgg tggcaggcgg
    42781 cgcggtaaaa ccgttaatgc gtgagttgat tatcgcggac aatgtgcacg gcgaaagcgg
    42841 tctcgacggc ccggcattac cggaaccgac gttcgcaccg caaaactgta cggcggtgga
    42901 gctgatggcg aaaacgctgc gcgaaagtgc ggaacctgtc acgattgttt ctaccggacc
    42961 gcaaactaac gttgccttgc tgctcaatag ccatccggaa ctgcatagca aaattgcccg
    43021 tatcgtgatt atgggcggcg caatggggct tggtaactgg acgcctgcgg ctgaatttaa
    43081 tatttacgtt gacccggaag cggcagaaat tgtcttccag tcagggatcc cggtggtgat
    43141 ggccggtctg gatgttactc ataaagcgca aatccacgtt gaagacaccg agcgtttccg
    43201 cgcgattggc aaccctgttt caaccattgt tgccgaactg ctggatttct tcctcgaata
    43261 tcataaagac gaaaaatggg gctttgtcgg cgcaccgctg catgacccat gcaccatcgc
    43321 ctggctgttg aaacccgagt tgtttaccac tgttgagcgc tgggttggcg tggaaacaca
    43381 ggggaaatat acccagggta tgacggttgt tgattattat tatctgactg gcaataaacc
    43441 gaatgccact gtaattgtcg atgttgatcg tcagggcttt gttgatttac tggctgatcg
    43501 tctgaaattt tacgcttaac actcgtcatt gattaagatc accacgcgtt acctgggtgg
    43561 tcttaatcta tttatctctt tttctttcaa gatataaatg caatggataa tgcagaactc
    43621 gccataggta tcgatctcgg cactaccaat agtttaattg ccgtctggaa agacggtgcc
    43681 gcgcaattaa ttccaaataa gttcggtgaa tatttaacac catccataat tagcatggat
    43741 gaaaataatc atattttagt cggaaaaccg gctgtatcac ggcgtacttc gcatccggat
    43801 aaaacggcag cgttatttaa acgtgcaatg ggcagtaata ccaactggcg gttaggcagc
    43861 gacacattta acgcgccaga actgtcctct ttggtattac gctcattaaa agaagatgcc
    43921 gaagaatttc tgcaacgtcc gattaaagat gtggtgatct ccgttctggc ttatttcagc
    43981 gatgaacaac gcaagcatac ccgtttagca gcggacttag ccgggttaaa tgcggtacgc
    44041 ttaattaatg aacccacagc agctgcgatg gcgtatggcc tgcataccca acaaaatacc
    44101 cgttcgctgg tgtttgatct cggtggcggc acgtttgacg ttacggtgct tgagtacgcc
    44161 acgccggtga ttgaagttca cgcctccgct ggcgacaact tcctcggtgg cgaagatttt
    44221 acccatatgt tggtcgatga cgttttaaag cgcgcggatg tcacaaggac cacgcttaac
    44281 gagagtgaac tggcagcctt gtacgcctgt gtggaagcgg caaaatgtag caatcaatcg
    44341 ccaatgcaca ttcgctggta gtaccaggat gaaacgcggg aatgcgaatt ttacgagaac
    44401 gaactggaag atttgtggtt gccgctgctc aatcgcttgc gagtgccgat tgaacaggcg
    44461 ttgcgcgatg cgcgtctgaa gccgagtcaa atcgacagtc tggtgctggt tggcggcgcg
    44521 tcacaaatgc cgctggtgca gcgaatcgcc gtgcgtctgt ttggcaaatt accgtatcaa
    44581 agttacgatc cgagcaccat tgtcgcgctg ggcgcagcaa tccaggccgc ctgccgctta
    44641 cgcagtgaag atattgaaga agtgatcctc actgatattt gcccttactc gttgggcgtt
    44701 gaggttaacc gccagggcgt ttccggcatt ttctcgccga ttattgaacg aaacaccact
    44761 gtgcccgtgt cgcgtgtaga aacttattca accatgcacc cggaacagga ttcaattacg
    44821 gttaacgtct atcagggaga aaaccacaaa gttaaaaaca acattctggt ggaatccttc
    44881 gatgtgccgt tgaagaaaac cggggcttat cagtcgattg atattcgctt tagttatgat
    44941 atcaacgggt tgcttgaagt tgacgtgctt ctggaagacg gcagcgttaa gtccagagtg
    45001 attaaccaca gcccggtaac attgagcgcg cagcagattg aagagagtcg gacgcggtta
    45061 tccgcattga aaatttatcc gcgcgatatg ctcatcaatc gcacctttaa agccaaactg
    45121 gaagagttgt gggcgcgggc gctgggtgac gagcgagaag agattggccg ggtgatcacc
    45181 gattttgatg cggcgctgca gtcaaacgat atggcccgcg tcgatgaggt caggcggcgg
    45241 gcgagcgttt atttagccat tgagacctca taatcacggc tgccggatgc ggcgtaaatg
    45301 ctgcatccgg catcggcgca aaaaacacat cgtcaactaa acaacatcgc gattttgccg
    45361 agagcataac ctataaccag caccgttaaa ctgattttga taatgctggt aatgctctgt
    45421 ggtttttcac cctcatcatt tttttcttca tggttttcag gtgttggctc atcgacatct
    45481 tcatcctccg gctcgagttt tactttcgtc aacagcatcc gggtgataat ggcctctaat
    45541 ggatgagcat ctccgccttc cagccttgta ttaagacggg ccagcatctc ttgtttcgga
    45601 aacgcttcaa gtactacctg ttccgctacc cgatacaaat ccagtaacgg tgaatcttct
    45661 tctgtctcgc taccgactct cgatttccac cacgcccatg ctttgcgttt gaagaataac
    45721 gactcaataa acgattgacc taacggcgat aattttccgc tatgtaaaat ttccgcccat
    45781 aacagccgcg tccgggcgct ctcaccagga acatatttca gcggttgacc ttgcgcatct
    45841 accatgctcc gcgcttcgat aaccatcact aataacgcga aataatccgg gcaatgacgc
    45901 ttgctccact gcaacaacaa attatcagct tgtgtagatg ggtaactttc ccagtacgcg
    45961 ccgagatcag caagcaggct ttccccctcg ccgcagtaca aacgttgtgc acactggtaa
    46021 taccgggcat cttcgctttc tggttcttcc gcttgccact gtttggctac aggaatgagt
    46081 tccaccatac ctgtttgcac cgagctgtac cagcgcaaca gtttgcgatg taactttgca
    46141 tcatcaggaa tgaaccacgg tccttccatc gccaaaaacg cagtgacata gtcaggatgg
    46201 taacggcagc aacgttccag cgcgaagtaa tattcaacag tctggttctg taacgcgacg
    46261 ggatattcca gaaggctaag gaaatcgaaa caatcaccag cctgaatggc ctcaagaaaa
    46321 gcctcccttt cttcctcatc cattccttcg ctgtcattaa acgaatgcca gttcaaacgc
    46381 tctgccaaca aagaagcaca atttaatgac agatatcgcg cttctatagc gattgcacac
    46441 agcggccagc gtaattgatc gacatcctcc atgttccagg tatttaattg ctgaataaat
    46501 ttctgccagg cggagggctg aaaacgatca ctttctgaat ccagtaatgt cctgaatgca
    46561 cgtagtattt catgttcagc ggtggcatct ttttcttcat catctgcttc ctcgacagga
    46621 tttaccgcca gtcgcagcgc ctcttcatag gcctggcgta atgctttaaa cccctgcgga
    46681 tcggtttcgg gatgacacaa cggtaagcga gcaagataag cctggcggat aatgtctatt
    46741 tgcgtcgtgc tttcaatttc gaggatttgc caacatgttt tcataggtcg ctcacatcct
    46801 taagcgttac cgggcattca ggctggacag ttaatggctg ccagggaagc gtggcataga
    46861 aacggtcgct tcccgctgcc agctcttcga acatccgact cccggcgcta tcgtatttgt
    46921 acagggttag cgcctcgcgt agcgaagcgt tgtcttcgca ctgagaagat tgccagtaga
    46981 gacgccctag tgaataggca gaaaaatagt gcatccagct gtgatagtaa tggtgagctc
    47041 gtgcatagac gcgcgattgc agccataaac tctcttcttc cgtcagccag ttgttgagca
    47101 caccggcccg tgacagaaat cccattcgaa cataatccca ggctttgatt cccccttctc
    47161 cacagcacat agcggtttgg gcaacgaatt gggtataaat caaccctctc tccgagccac
    47221 cagcaattaa tgctttccac tcttcagggg aagagcgttg ccatgtcaga tataagccag
    47281 ctaaatcatt ggcatgacca tcatcagtca tctgataaat agtctgaatt aaatcatcac
    47341 gattatttat tccccaggat gatgagagat cgaattcttt tatcccggta tacatttttg
    47401 gcgagtcatg cgcgggtttg caactctgat taagtatttc catgggcgca gaaagcgcaa
    47461 aaagaagata ttgttcttcc ttattcattt tttaataatc ccttaagtgc attaactgac
    47521 tactttttta ttattgatga gtataaactt gacgctttca aaataaaaag aaaatcgaag
    47581 cattcacaca tgaataaaaa attaatgtat atattcgcaa tttttatagt tgcagcaatt
    47641 acctgtatta gccaacccaa gaaaacgatg ttgcgtgata aagccatggt gaattatgcc
    47701 tttgattatt taagatcacc aggcagtcta ccgttaacca cggcagccac ggagctttcc
    47761 gcgattcatg gtcactcaac atcgcaatat cgccttggcg aattttatct tcatggtagc
    47821 gacggtaaac cactggatta tacacaggcg agatactggt atgagcaatc agcagaacag
    47881 gaaaatccgc gcgcacaaag caaactgggg tggatctacc tgaaaggcct gggagtcaaa
    47941 cccgataccc gtaaagcaat tctctggtat aaggaagcag ctgaacaagg gtatgctcat
    48001 gctcaatata ctttgggttt gatctacaga aatggcactg gtattaatgt taaccattat
    48061 gaatctcaaa aatggttaaa actggccgcc aaacaacatt acaaaaatgc ggaaagatta
    48121 cttgccggac ttcccgcaca ttaacgtcga ctgtcacccg aagagatgga gtattttccc
    48181 tatcaaaccg gcaaagataa aaatgtagaa aataattttg atgacgccca gacagcccag
    48241 cccgccatca tcaacgggca ttggctgcgg ttgcggctcc ggctcgacgg tttcctgacc
    48301 ggtaaaattc gccgcctgaa ttaataatcg acgaataatt aatgcttcca gtggacaacg
    48361 atcttctgca tcaaggcggc tcacaatcgg cattaatgat tcttctgtcg gttggctggc
    48421 ctgcactaat tgtttggcaa ctaaatataa tggccataag ggatgttctg tatcacgcgt
    48481 gagtgagatc cgcggcttac gtcctttgtg caaaaccatc tcgaccagtg aacgccccag
    48541 cgcagagagt ttttcatcgc tgagtatttc gtataaatga aaacgcgtac gggcgctgtc
    48601 gccggggaca taaagtagcg gttttccctg atcgttaacc agatcacgcg cttcaatcat
    48661 catcaccact aatgggtaat aatagacccg atgctgacgg caccattgca acattaaagc
    48721 atccgcctgg gtggagggat actcgcgcca gtagtaacaa agttctgcca gcaggctttc
    48781 gccttctcca caatagacgc gttgtgcgta ttcataataa cgcgcatctt cattatcagg
    48841 ttcagcttcc cgccacgacc gggcgtaatc caggagttcg gggattccca ggcggcttgc
    48901 gctataccag cggagtaaat ttctgtgcag ttttgcatca tcggggataa tcactgcgcg
    48961 atgttgcgct aaatacaatg tcagccattc tggatgcgat gaccaaatcc gagcgaacat
    49021 ttggtaaaaa tcgatggtgt cattttgcac ggcaactggc agatgcagaa tggtctggaa
    49081 gttaaaaaca ttccccttcg caatggcata taaaaaggat tccagttctt cttcgtctat
    49141 ttccccgacg ttttcctcct gcaaccaccg caatctttct gctaataaca ccacgcactc
    49201 gaaggataaa tgggcagtgt tcatggcgat tgtgcacagc gaccagcgta attcatcaat
    49261 ctcttccatc gagcaataat ttaattgctg aatgaatcgc tgccaggcgg agggcagaaa
    49321 acgttcgcta tcagaggcaa gtaacgcgcg aaaggcgagc agaatttcat gttctgctac
    49381 ctcatattct tctggttgcc aaacagattt agcaggcgac tgcgcaatcc gtagcgcctc
    49441 ctcatacgcc tgacgaagtt gtttaaaacc ctgcggatcg gtttctggat gaaaggacgg
    49501 taacagcgcc agataagcgc ggcggataat atcgacgtca gtcgtttcct ctatatcgag
    49561 gatcttccag caatttttca taagtcgctc atatccttaa gcgtttccgg gcatgtgggg
    49621 tagtcagcca gtggttgcca ggacaaggta gggtaatacg catcatcact ggcaaataat
    49681 tgataaaaca tatcattgcc ggaatcgtcg tattctttac gcgctaagaa ttcgcgtaat
    49741 aaaggcagat gatcatcttc gggagactgc caatattgcc gaccaaaggt ataaccggca
    49801 aagtattgcc gccagttact ataataacgt agagcccgta gatgaatacg cgactggatc
    49861 catagacttt cttcttcact caaccagtta ttaagtacgc ccattcgact taaaaaaccc
    49921 atccggacat aatcccatgc tttaattcct ccctcaccac agcattcggc tgtgctggcg
    49981 acaaattgcg cataagcctg accttgctca tttaattcag caagatagtc acgccattca
    50041 catggagaat aacgaaacca gcggtgataa aaccctgcca accgagcggc atgaccgttc
    50101 tcgatcattc ggtgaattat gcgcagcagg tcatcacggt catcaattcc ccatgaggaa
    50161 gaaaggtcaa aaaagcatgt acctaaatac attttggggg atgaatgcga aggtaaacag
    50221 cattcattgc gtatttccat cggagtggaa agtgcaaaca atattttttg cgaccccatg
    50281 tccatataat ttatccttaa acattcattt aatgaatgta aactacattt cgaggctcag
    50341 ttatttatac ttttctgacg caacataata cgcataaaat aaggcttacg caaaaatgga
    50401 acacataaaa atataatata ttttatctac aaccataagg aaatgataat gatttttacg
    50461 tcaagttgct gcgataattt atcaatagat gagattatcg aacgtgctga aaaaggcgat
    50521 tgcgaggctc aatatattgt tgggttttat tataatcgcg atagcgcaat tgactctccg
    50581 gacgacgaaa aggcctttta ctggctgaag ctggccgctg agcaaggtca ttgtgaagca
    50641 cagtattcct tagggcaaaa gtataccgag gataaaagcc gtcataaaga taatgagcaa
    50701 gccatcttct ggctgaaaaa agctgcccta caaggccata ctttcgcttc caacgccctt
    50761 ggctggatac tggatcgtgg agaagacccc aactataaag aagcggttac ctggtatcag
    50821 atagccgcgg agagcggaat gtcttatgcg caaaataatc ttggatggat gtacagaaat
    50881 ggcaacggag tcgcacaaga ctatacgctg gcatttttct ggtacaaaca agctgcatta
    50941 caaggccata gttacgcaca agacaatctg gccgatcttt ataaagacgg agaaggcgtt
    51001 gctcaaaaca agacactcgc cgcattctgg tatttgaaaa gcgcacagca gggtaatcgg
    51061 cacgcccagt ttcaaattgc atgggattat aacgctggcg aaggggtgga ccaggactat
    51121 aagcaagcga tgtactggta tctgaaggct gccgctcagg aaagcgtcgg cgcttacgtc
    51181 aacatcggtt atatgtataa acacggacaa ggcgttgaga aagattatca ggctgccttt
    51241 gaatggttta cgaaagccgc tgaatgcaat gacgccactg cctggtataa cctggccatt
    51301 atgtatcact acggagaagg aagacctgtc gatctccgac aggctctcga cctgtatcgt
    51361 aaagttcagt catccggaac cagagatgtc agtcaggaaa ttcgtgagac tgaagcttta
    51421 ctgtagaaat aaaaacaagg gccggggaat ttcattctgc ccatattctc tttattgctg
    51481 tgcgaaatca gactattacg gctcaaacgg gcgtctctgg aactggtcat aaccacattt
    51541 cgggcatagc gtcagcactt ccggtgtgta gatcgggaga tggaagtgac atttctcgca
    51601 gaccagattt cccagcccga ccacttctcc gctgtgataa accccatgat gattgaggtc
    51661 ctggaaaatt tcgcgccatt caagctgcgt tttatcggtg atgtctgcca gctcctgcca
    51721 caagctttct ttaatcaccc gcataaagac gctgtcagat tcttctttca ggctctcttc
    51781 atagctcatg gcgaactctt ccaggtcacg tctgacagct cgcgtcagct catcgatctc
    51841 ggttcgcgtt aactcccctg tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc
    51901 gatatcacgt tcgccattgc gcaggcgttc gttcagtgac gcgaccagtt cacggtaata
    51961 ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta actgactata ataatagacg
    52021 ttctttgttc cttgctttgc taatacgccc acaaactctg taaattaatg tacgaataat
    52081 ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt tgacgcggac gtttgggcta
    52141 tgctatgcgg atctgaaaaa ccacctcaac gctacatttg tagccgtatt gaaaacagga
    52201 ccactggctg ccatgcaaga gcaataccgc ccggaagaga tagaatccaa agtacagctt
    52261 cattgggatg agaagcgcac atttgaagta accgaagacg agagcaaaga gaagtattac
    52321 tgcctgtcta tgcttcccta tccttctggt cgactacaca tgggccacgt acgtaactac
    52381 accatcggtg acgtgatcgc ccgttaccag cgcatgctgg gcaaaaacgt tctgcagccg
    52441 atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgct
    52501 ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa accagctcaa aatgctgggc
    52561 tttggttatg actggagccg cgagctggca acctgtacgc cggaatacta ccgttgggaa
    52621 cagaaattct tcaccgagct gtataaaaaa ggcctggtat ataaaaagac ttctgcggtc
    52681 aactggtgcc cgaacgacca gaccgtactg gcgaacgaac aagttatcga cggctgctgc
    52741 tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc agtggtttat caaaatcact
    52801 gcttacgctg acgagctgct caacgatctg gataaactgg atcactggcc agacaccgtt
    52861 aaaaccatgc agcgtaactg gatcggtcgt tccgaaggcg tggagatcac cttcaacgtt
    52921 aacgactatg acaacacgct gaccgtttac actacccgcc cggacacctt tatgggttgt
    52981 acctacctgg cggtagctgc gggtcatccg ctggcgcaga aagcggcgga aaataatcct
    53041 gaactggcgg cctttattga cgaatgccgt aacaccaaag ttgccgaagc tgaaatggcg
    53101 acgatggaga aaaaaggcgt cgatactggc tttaaagctg ttcacccatt aacgggcgaa
    53161 gaaattcccg tttgggcagc aaacttcgta ttgatggagt acggcacggg cgcagttatg
    53221 gcggttccgg gtcacgacca gcgcgactac gagtttgcct ctaaatacgg cctgaacatc
    53281 aaaccggtta tcctggcggc tgacggctct gagccagatc tttctcagca agccctgact
    53341 gaaaaaggcg tgctgttcaa ctctggcgag ttcaacggtc ttgaccatga agcggccttc
    53401 aacgccatcg ccgataaact gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc
    53461 ctgcgcgact ggggtgtttc tcgccagcgt tactggggcg cgccgattcc gatggtgacg
    53521 ctggaagacg gtaccgtaat gccaaccccg gacgaccagc tgccggtgat cctgccggaa
    53581 gatgtggtga tggacggcat taccagcccg attaaagcag atccggagtg ggcaaaaact
    53641 accgttaacg gtatgccagc gctgcgtgaa accgacactt tcgacacctt tatggagtcc
    53701 tcctggtact atgcgcgcta cacttgccca gagtacaaag aaggtatgct ggattccgaa
    53761 gcggctaact actggctgcc ggtggatatc tacattggtg gtatcgaaca cgccattatg
    53821 cacctgctct acttccgctt cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct
    53881 gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc tggcagatgc cttctactat
    53941 gttggcgaaa acggcgaacg taactgggtt tccccggttg atgctatcgt tgaacgtgac
    54001 gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc atgaactggt ttataccggc
    54061 atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc cgcaggtgat ggttgaacgt
    54121 tacggcgcgg acaccgttcg tctgtttatg atgtttgctt ctccggctga tatgactctc
    54181 gaatggcagg aatccggtgt ggaaggggct aaccgcttcc tgaaacgtgt ctggaaactg
    54241 gtgtacgagc acacagcaaa aggtgatgtt gcggcactga acgttgatgc gctgactgaa
    54301 gatcagaaag cgctgcgtcg cgatgtgcat aaaactatcg ctaaagtgac cgatgatatc
    54361 ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta tggagctgat gaacaaactg
    54421 gcgaaagcac cgaccgatgg cgagcaggat cgcgctctga tgcaggaagc actgcaggcc
    54481 gttgtccgta tgctcaaccc gttcaccccg cacatctgct tcacgctgtg gcaggaactc
    54541 aaaggcgaag gcgatatcga caacgcgccg tggccggttg ctgacgaaaa agcgatggtg
    54601 gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag tccgtgccaa aatcaccgtc
    54661 ccggtggacg caacggaaga acaggttcgc gaacgtgctg gccaggaaca tctggtagca
    54721 aaatatcttg atggcgttac tgtacgtaaa gtgatttacg taccaggtaa actcctcaat
    54781 ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct ggcaacactg ttgttatctc
    54841 tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg tgacaccacg caggttcctt
    54901 ccactatgaa ggtcatgatc ctggactcag gcgatccgaa cgggccatta agccgtgcgg
    54961 tgcgtaacca gttacgtctg aatggtgtcg agttgcttga taaagaaacc acgcgtaagg
    55021 acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa agataccgca tcggtattcc
    55081 gtaacggtca aacagcagag tatcagatga tcatgacggt gaatgcgacc gtgttgatcc
    55141 ccggccgtga tatctacccg attagcgcca aagtcttccg ttcattcttc gataacccgc
    55201 aaatggcgtt agcgaaagat aacgaacaag acatgatcgt aaaagagatg tacgaccgtg
    55261 ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc tgcggatatt cgttccgacg
    55321 aagaacagac gtcgaccaca acggatactc cggcaacgcc tgcacgcgtc tccaccatgc
    55381 tgggtaactg atgattcggt tgtacccgga acaactccgc gcgcagctca atgaagggct
    55441 gcgcgcggcg tatcttttac ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc
    55501 tgttcgccag gtagctgcgg cacaaggatt cgaagaacac cacacttttt ccattgatcc
    55561 caacactgac tggaatgcga tcttttcgtt atgccaggct atgagtctgt ttgccagtcg
    55621 acaaacgcta ttgctgttgt taccagaaaa cggaccgaat gcggcgatca atgaacaact
    55681 tctcacactc accggacttc tgcatgacga cctgctattg atcgtccgcg gtaataaatt
    55741 aagcaaagcg caagaaaatg ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt
    55801 gacctgtcag acaccggagc aggctcagct tccccgctgg gttgctgtgc gcgcaaaaca
    55861 gctcaactta gaactggatg acgcggcgaa tcaggtgctc tgctactgtt atgaaggtaa
    55921 cctgctggcg ctggctcagg cactggagcg tttatcgctg ctctggccag acggcaaact
    55981 gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg catttcaccc cttttcattg
    56041 ggttgatgct ttgttgatgg gaaaaagtaa gcgcgcgttg catattcttc agcaactgcg
    56101 tctggaaggc agcgagccgg ttattttgtt gcgcacatta caacgtgaac tgttgttact
    56161 ggttaacctg aaacgccagt ctgcccatac gccactgcgt gcgttgtttg ataagcatcg
    56221 ggtatggcag aaccgccggg gcatgatggg cgaggcgtta aatcgcttaa gtcagccgca
    56281 gttacgtcag gccgtgcaac tcctgacacg aacggaactc accctcaaac aagattacgg
    56341 tcagtcagtg tgggcagagc tggaagggtt atctcttctg ttgtgccata aacccctggc
    56401 ggacgtattt atcgacggtt gatatgaaat ctttacaggc tctgtttggc ggcacctttg
    56461 atccggtgca ctatggtcat ctaaaacccg tggaaacgct ggcgaatttg attggtctga
    56521 cgcgggtcac aatcatccct aataatgttc ctccgcatcg tccccagccg gaagcgaaca
    56581 gcgtgcagcg taaacacatg cttgaactgg cgattgccga caagccatta tttactcttg
    56641 atgaacgcga gctaaagcgc aatgccccct cttacactgc gcaaacactg aaagagtggc
    56701 ggcaggaaca aggaccggac gtgccgctgg cgtttattat tggtcaggat tcattgctga
    56761 cctttccgac ctggtacgaa tacgaaacga tactcgacaa tgcacatttg atcgtctgtc
    56821 ggcgtccagg ttacccactt gaaatggcgc aaccgcaata ccagcaatgg ctggaagatc
    56881 atttgacaca taacccggaa gatcttcacc ttcagcctgc cggtaaaatt tatctggctg
    56941 aaacgccgtg gtttaacatc tcggcgacca tcatccgcga acgtttgcaa aacggtgaat
    57001 cgtgtgagga tttattgccg gaaccggtat tgacttacat taaccaacaa ggcttgtatc
    57061 gctgatacct gtcgttacgg agggcatgat gcgactgtgg ttaattcgtc atggcgaaac
    57121 acaagcgaat gtcgatggtc tgtacagcgg tcatgcgccc acacccctga ccgcacgcgg
    57181 tattgagcaa gcgcaaaatc tgcatacact gctagatgat gtttcctttg acttggtttt
    57241 atgcagtgaa ctggaacggg cacagcatac cgcgcgactg gttctcagtg accgccagct
    57301 ccccgttcat atcatccctg aactcaacga aatgtttttt ggcgactggg agatgcgaca
    57361 tcatcgcgac ctcatgcaag aagatgccga aaactatagc gcgtggtgca atgactggca
    57421 gcatgctatc cccacgaacg gtgaaggatt tcaggcattt tcgcaacgtg tggaacgctt
    57481 tatcgcaagg cttagtgaat atcagcacta tcagaatatt ttaatcgtca gccatcaggg
    57541 cgtactgagt ctgttaattg ctcgtttaat tggcatgcct gccgagtcta tgtggcattt
    57601 tcgcgttgac caggggtgtt ggagcgccat tgatatcaac caaaaattcg cgacgctacg
    57661 cgtcctcaat agccgtgcca tcggggtcga gaacgcatga cttttctgtt tttttacggg
    57721 taagccgcaa cgaccattga cagccccggg caggctgata ttctccgcag ccagactttt
    57781 tccgccagac acgactttgt agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc
    57841 ggggtgggat gatagcccac tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc
    57901 tctggttcag gtatactgac agaccatttt tatctatttg attcacccag ggggaaaact
    57961 tgcagggtaa agcactccag gattttgtta tcgacaaaat tgatgacctc aaaggtcagg
    58021 acatcatcgc cttagacgtt caaggcaaat ccagcatcac cgactgcatg atcatctgta
    58081 cgggtacgtc cagccgtcat gttatgtcca ttgctgacca cgttgtgcag gagtctcgcg
    58141 cagcgggcct gttaccgctc ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg
    58201 atttgggcga tgtgattgtc catgtcatgc aggaagagag ccgtcgcctg tatgaactgg
    58261 aaaaactctg gagttaatgc gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg
    58321 actgggtaca aaccggtttt accgagtacc tgcgtcgttt tccgaaagat atgcccttcg
    58381 agctgattga aattccggcc ggcaaacgcg gcaagaatgc ggacatcaag cgcatactcg
    58441 acaaagaggg tgagcagatg ttggcggccg caggcaaaaa ccgcattgtc accctcgata
    58501 ttccaggcaa gccctgggat acgccgcagt tagccgctga gctggaacgc tggaagctgg
    58561 atggtcgcga cgtaagtcta ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag
    58621 cggcggctga gcagagctgg tcgctgtcgg cgcttaccct cccccatccg ctggttcgcg
    58681 tgctggtcgc agagagtctg taccgggcgt ggagcatcac caccaaccat ccttatcacc
    58741 gtgagtgata agggagcttt gagtagaaaa cgcagcggat gaaactacag aactcttttc
    58801 gcgactatac ggcagagtcc gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga
    58861 ttttgctgct gaccggcgtg cttatcgcca acctgtataa tctgcaaatt gttcgcttta
    58921 ccgactacca gacccgctct aatgaaaacc gcattaagct ggtgcctatc gcgcccagcc
    58981 gcggcattat ctacgaccgt aacggtatcc ctctggccct caaccgcact atctaccaga
    59041 tagaaatgat gccggagaaa gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg
    59101 tggtagatct gaccgatgac gatattgctg cattccgaaa agagcgcgca cgttcacacc
    59161 gtttcacctc tattccggtg aaaactaacc taaccgaagt acaagtagct cgctttgccg
    59221 tcaatcagta ccgttttccg ggtgtcgaag ttaaaggcta taaacgtcgt tactatcctt
    59281 acggttcggc gttgacccac gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg
    59341 aacgcctgaa taatgacggc aaactggcca actatgcggc aacgcatgat attggtaagc
    59401 tgggcattga gcgttactat gaagacgtgc tgcacggtca gaccggttat gaagaggttg
    59461 aagttaacaa ccgtgggcgt gttatccgcc agttaaaaga agtaccaccg caagccggac
    59521 acgatattta cctgacgctg gatctcaaac tccagcaata tattgaaacg ctgctggcgg
    59581 gtagccgcgc agctgtggtt gttaccgaac cgcgtacagg tggggtgctg gcgctggttt
    59641 ccacgcctag ttatgaccca aacctgtttg ttgacggtat ctccagcaaa gattattccg
    59701 ccttgctgaa cgacccgaat acaccgctgg tgaaccgcgc cacacagggg gtttatcctc
    59761 ccgcgtctac agttaaaccc tatgtggcgg tttcggcatt aagcgccggg gtgatcacgc
    59821 gcaatacgac gctgtttgac ccaggctggt ggcaactacc agggtcggaa aaacgttatc
    59881 gtgactggaa aaaatggggc cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg
    59941 cggatacctt cttctatcag gtggcctacg atatggggat cgatcgcctc tccgaatgga
    60001 tgggtaaatt cggttatggt cattacaccg gtatcgacct ggcggaagaa cgttccggca
    60061 acatgcctac ccgcgaatgg aaacagaaac gctttaaaaa accgtggtat cagggtgaca
    60121 ccattccggt tggtatcggc cagggttact ggacagcgac cccaatccag atgagtaagg
    60181 cactgatgat cctgattaat gacggtatcg tgaaggttcc gcatttgctg atgagcaccg
    60241 ccgaagacgg caaacaggtg ccatgggtac aaccgcatga accgcccgtc ggcgatattc
    60301 attccggtta ctgggagctg gcgaaagacg gtatgtacgg tgttgctaac cgccctaatg
    60361 gtacggcgaa taaatacttt gccagcgcac cgtacaaaat tgcggcgaaa tctggtaccg
    60421 ctcaggtctt cggtctgaaa gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt
    60481 tacgtgacca caaactgatg accgcctttg cgccatacaa caatccgcaa gtggctgtcg
    60541 ccatgattct ggagaacggc ggcgcgggtc cggcggttgg tacgctgatg cgccagatcc
    60601 tcgaccacat tatgctgggt gataacaaca ccgatctgcc tgcggaaaat ccagcggttg
    60661 ccgcagcgga ggaccattaa tcatgacgga taatccgaat aaaaaaacat tctgggataa
    60721 agtccatctc gatcccacaa tgctgctgat tttactggca ttgctggttt acagcgccct
    60781 ggttatctgg agcgccagcg gtcaggatat tggcatgatg gagcgtaaaa tcggccaaat
    60841 cgcgatgggt ctggtcatca tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg
    60901 ctgggccccc tatctctata tcatctgtat tattttgctg gtggcggtag atgctttcgg
    60961 agccatctct aaaggcgctc aacgctggct ggacctcggt attgttcgtt ttcagccgtc
    61021 ggaaattgcc aaaatagccg taccactgat ggttgcgcgc tttatcaacc gcgacgtttg
    61081 cccgccatcg ttgaagaata ctggcatcgc gctggtgctg atatttatgc ccacgctgct
    61141 ggtggctgca cagcctgacc tgggaacatc aatcctcgtt gcgctttccg gtctgtttgt
    61201 actgttcctc tctggcctta gctggcgtct gattggcgtc gcagtagtgc tggtagcggc
    61261 gttcattccg attctgtggt tcttcctgat gcatgattac cagcgccagc gcgtaatgat
    61321 gctcctggac ccggaatcag acccactcgg cgcgggctat cacattattc agtctaaaat
    61381 tgctattggc tccggcggat tacgcggcaa aggctggctg cacggcactc agtcacagct
    61441 tgaatttctc cccgaacgcc atactgactt tatcttcgcg gtactggcgg aagagctggg
    61501 attagtgggc attctgattc tgctcgctct ctacattctg ctgatcatgc gcgggctgtg
    61561 gatagccgcc agagcgcaaa ccacctttgg tcgcgtcatg gctggtggct taatgctgat
    61621 attattcgtt tatgtcttcg taaatattgg tatggtaagc ggtattctgc cggttgtagg
    61681 ggttccgctc ccactggtca gttatggagg atcggcgcta attgtgctga tggctgggtt
    61741 cgggattgta atgtcaatcc acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt
    61801 gcgcaatgcg taagcagtgg ctcgggatct gcatcgcggc aggaatgctc gcggcatgta
    61861 caagcgatga tggtcagcaa cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc
    61921 ccatagttga aattagcggg gcggacccgc gtttcgaacc actgaacgcg acggcaaatc
    61981 aggattacca gcgcgacggt aaaagctaca aaatcgtgca ggatccgtct cgatttagcc
    62041 aggcgggact ggcggcaatc tatgatgccg aaccaggcag taacctgacg gcctctggcg
    62101 aagctttcga tccgacaaag ctgacggcgg cccatccaac gcttccgatc cccagctacg
    62161 ccagaatcac taacctggct aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc
    62221 cttacggcaa cgaccgcgtt atttcgcttt ctcgcgcggc agctgaccgt cttaacacgt
    62281 caaacaacac caaagttcgt atcgatccga ttattgtcgc ccaggatggt tcgctttctg
    62341 gtcctggtat ggcttgtacc acagtcgcca aacagactta cgccctgcct gcacctcccg
    62401 atttaagcgg tggcgcggga acaagttcag tgtctggccc gcagggtgac attcttccgg
    62461 tcagtaattc gacgctaaaa agcgaagatc cgaccggcgc gccggtaacc agtagcggtt
    62521 tcctcggcgc tccaacgacc ttagcgcctg gtgtactgga aggcagcgaa ccgacgcctg
    62581 cgccacagcc cgttgttaca gcttcgtcga caacgcctgc aacctcgcct gcaatggtga
    62641 caccgcaagc cgcctcgcaa agcgccagcg gcaactttat ggtgcaagtc ggggccgtaa
    62701 gcgatcaggc tcgtgcgcaa cagtaccaac agcaactggg acagaagttc ggcgtccccg
    62761 gtcgcgtaac tcaaaatggc gcggtctggc ggatccagct tggcccattc gccagcaaag
    62821 ccgaagccag taccttgcag caacgtttgc aaaccgaagc ccaattacag tcatttatta
    62881 ctaccgcgca gtagcgtaaa gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac
    62941 aaagtcacgc ggaaagtcag atgcctgccg gtagtgcatt tgctatagta gggcactttt
    63001 ttaattccat cacggatgtc gtagttcaga ccatgaatac cattttttcc gctcgtatca
    63061 tgaagcgcct ggcgctcacc acggctcttt gcacagcctt tatctctgct gcacatgccg
    63121 atgacctgaa tatcaaaact atgatcccgg gtgtaccgca gatcgatgcg gagtcctaca
    63181 tcctgattga ctataactcc ggcaaagtgc tcgccgaaca gaacgcagat gtccgccgcg
    63241 atcctgccag cctgaccaaa atgatgacca gttacgttat cggccaggca atgaaagccg
    63301 gtaaatttaa agaaactgat ttagtcacta tcggcaacga cgcatgggcc accggtaacc
    63361 cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt ccggtttctc
    63421 agctgatccg cggtattaac ctgcaatcgg gtaacgatgc ttgtgtcgcc atggccgatt
    63481 ttgccgctgg tagccaggac gcttttgttg gcttgatgaa cagctacgtt aacgcactgg
    63541 gcctgaaaaa tacccacttc cagacggtac atggtctgga tgctgatggt cagtacagct
    63601 ccgcgcgaga tatggcgctg atcggccagg cattgatccg tgacgtaccg aatgaatact
    63661 cgatctataa agaaaaagaa tttacgttta acggtattcg ccagctgaac cgtaacggcc
    63721 tgttatggga taacagcctg aatgtcgacg gcatcaaaac cggacacact gacaaagcag
    63781 gttacaatct tgttgcttct gcgactgaag gccagatgcg cttgatttct gcggtgatgg
    63841 gtggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa actgctaacc tggggcttcc
    63901 gtttcttcga aaccgttaac ccactgaaag taggtaaaga gttcgcctct gaaccggttt
    63961 ggtttagtga ttctgatcgc gcttcgttag gggttgataa agacgtgtac ctgaccattc
    64021 cgcgtggtcg catgaaagat ctgaaagcca gctatgtgct gaacagcagt gaattgcatg
    64081 cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt ccagcttgat ggcaaaacga
    64141 tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga aggtaacttc ttcggcaaaa
    64201 tcattgatta cattaaatta atgttccatc actggtttgg ttaaaaattg aacacttgaa
    64261 agtgtaattt ccgtccccat atactaagca tcagtaaaaa aactcccgcc ttctggcggg
    64321 agttgctatt taattacgtt acgccggagc tgacatgaaa accaaactta acgaactgct
    64381 tgaattccct actcctttta cttacaaagt tatggggcag gcgttacctg agctggttga
    64441 tcaggtggtt gaagtggtac agcgccatgc gccaggtgac tacaccccaa cggtaaaacc
    64501 aagcagcaaa ggcaactacc actcggtatc tatcactatc aacgccactc atatcgagca
    64561 ggttgaaaca ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg ttctgtaact
    64621 cgcttctccg ttacccggct cctggtcggg taactcccct ctcctcgctg tgatatactt
    64681 ttcccccact tttactcatt ctccacggag atgccgtttt gtatcaggat aaaattcttg
    64741 tccgccagct cggtcttcag ccttacgagc caatctcccg ggctatgcat gaattcaccg
    64801 atacccgcga taatagtacc cttgatgaaa tctggctggt cgagcactat ccggtattca
    64861 cccaaggtca ggcaggaaaa gcggagcaca ttttaatgcc gggtgatatt ccggtgatcc
    64921 agagcgatcg cggtgggcag gtgacttatc acgggccggg acaacaggtg atgtatgtgt
    64981 tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg cttgagcaaa
    65041 cagtggtgaa taccctggct gaactgggta tagaagcgca tcctcgggct gacgcgcctg
    65101 gtgtctatgt cggggaaaag aaaatttgct cactgggttt acgaattcga cgcggttgtt
    65161 cattccacgg tctggcatta aacgtcaata tggatctttc accattttta cgtattaatc
    65221 cttgtgggta tgccggaatg gaaatggcta aaatatcaca atggaaaccc gaagcgacga
    65281 ctaataatat tgctccacgt ttactggaaa atattttagc gctactaaac aatccggact
    65341 tcgaatatat taccgcttaa ttccatacat caatggccca atttacactg ggtcattact
    65401 cataatacct gctaatgctt tacatctcct tcgcgtttgt atattttctt tatcacttgc
    65461 agaaatgcgg tgccacgcct tgctccgtaa ctcacctcat tgattaacct attgaaatag
    65521 ttgattatgg tgagcaaaag cgttccgttc caattagaca taagattatt gtaatttttt
    65581 aaatattatt tttccatgaa taaaattgga gtaagtgcgt ggatagtaat aatcaaattg
    65641 aaccctgttt aagcagaaag tcgtcagaag gtaaaccaca aatatttaca accttacgaa
    65701 atattgatct taaccttctg actatttttg aagctgtata tgtacataaa gggatcgtta
    65761 atgcagcgaa agtgcttaat ctgaccccct cggcaatcag tcagtctatt cagaaactgc
    65821 gcgttatatt ccctgaccca ttgtttattc gcaaaggcca gggtgtcact cctaccgcat
    65881 ttgcgatgca tctacatgag tatatcagtc agggccttga gtccattctt ggcgcgctgg
    65941 atatcgaagg aagctatgat aagcaacgaa cgataactat tgcgaccact ccctcggtcg
    66001 gagccctggt ccttcctgtc atctatcggg cgattaaaac tcactatccg cagcttttac
    66061 tgcgcaaccc gcccatcagc gacgcggaaa accaactcag tcagtttcaa accgatctca
    66121 tcatcgataa catgttttgc accaatcgta cggtgcaaca tcatgttctg ttcaccgaca
    66181 atatggtgtt aatttgccgc aagggaaatc cactactctc tttagaagat gacagagaga
    66241 ctatcgacaa cgctgcgcat gtacttctgt taccggaagg gcaaaatttc agcggtctgc
    66301 ggcagagagt tcaagagatg tttccggacc ggcaaatcaa tttcaccagc tacaacattt
    66361 tgacaatcgc tgcactggtt gccaacagtg acatgttagc gattattccc agccgttttt
    66421 ataacctgtt tagtcgttgc tggccgctgg aaaaactgcc ttttccgtcc ttaaatgagg
    66481 agcaaataga tttctctatc cactacaaca aattcatcct gcgtgatccc atcctgcacg
    66541 gagtaataga tgttatccgt aatgcatttt gaaaaagtat tgttacagcc agatcacagc
    66601 gcagaacgat aaaatggcac aatccaacaa caattttaca ttttcgcgac cgctttggct
    66661 gctttcaggt ccgtttcaat gatatactgc cagtcgttaa ttcaaaaata gttgataatt
    66721 acaacaatct attgaattga aacgctttcc ttcgtaattc gcaactggaa cacgcacgct
    66781 atgagtaaac ccattgtgat ggaacgcggt gttaaatacc gcgatgccga taagatggcc
    66841 cttatcccgg ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg
    66901 atgaaaatca agcttccagc ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc
    66961 aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc
    67021 aaccacggca cagcaacgtt tatgatcctc ggcgctattt gtacccgccg ttgtccgttc
    67081 tgtgatgttg cccacggtcg cccggtagct cctgatgcca atgaaccagt gaaactggcg
    67141 cagaccattg ccgatatggc gctgcgttat gtggttatca cctccgttga ccgtgatgac
    67201 ctgcgcgatg gcggtgccca acactttgcg gattgtatta ctgccattcg ggaaaaaagc
    67261 ccgcaaatca aaattgaaac tctggtgccg gatttccgcg gtcgaatgga tcgtgctctg
    67321 gatattctga ctgcaacgcc cccagatgtg ttcaaccata acctggaaaa cgtaccgcgt
    67381 atttaccggc aggtacggcc tggcgcagat tacaactggt cgctgaagct gctggaacgc
    67441 tttaaagaag cgcatccgga aatcccgacc aagtctggtc tgatggtggg tctgggtgaa
    67501 accaatgaag aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta
    67561 acgctggggc aatatttgca gccaagccgc catcacctgc cggttcaacg ttacgttagc
    67621 ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga tgggctttac ccatgctgca
    67681 tgcggtccgt ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg aatggaagtt
    67741 aagtaatcct gtaataatta cttacatctg tctgataaaa aacccgcttt ttgaagcggg
    67801 ttttttgtat cgaacaagat attgagggag cgtcctgctc gccacgtcac tctttatgag
    67861 agagcttttc agcctgaaga tcaacgtctg cgcctttttt cgccgcagca tcgtcatcat
    67921 tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc tccgcccagc gtacgtaact
    67981 tcttagtccc aaacagcaga acgaccagcg ccgcaactac cagcagtttg gtaatactaa
    68041 tctcacccat agataccttc ttgacatata accggcaaca gaacgcgcct taaaaaccag
    68101 agaatattaa cggtcattcg acgcgcgcac acaatagcaa tctgtaacaa ggtgaatcaa
    68161 gcgccgagta aaaaaacatc ataataattg cggcggcgca aagcgacggt tgtttaagat
    68221 gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc acttccgcca taatgagtgc
    68281 aggcatttct gacgctgccg caatggtgac gccaaaggga tctataatcc ggctttgccc
    68341 gatatttttg ttcccgcact cccccgccgc caccatataa caggtggtat ccagcgcacg
    68401 agcggcaagc aacgttgacc agtgatgctc tttgagcggc ccacgaaccc aggcggcagg
    68461 aagtaccagg atttcagctc cctgtaaagc ctgtgccagc gccagctctg gaaagcgtaa
    68521 gtcataacag gtcatcagac cgaccttcat cccttccacc tccagtaacg gagctatttc
    68581 attaccagca tcaacatggc gtgattcctg aatggcaaat gcatcataga gatgcagttt
    68641 ggcataacgg gcgacgatgt ttcctgcctg aagtgccacc agcatattcc atgcgcgccc
    68701 cggcgttgaa ggaagatgaa tcgtcagaat tgtcgtcatc atgttacgtt tactttctcg
    68761 ccgtaaacgt ccgaggaatt cgccttccag cagctgtgct gatttaaccg atagatctgc
    68821 atcatgatca tcgcgcgcca gcaatgcttc cggcaggaca aacagcgata cgtcgttttc
    68881 cgccgcctgc gccatcaacg aggcacaaat ctcagcgttc ttttcccaca cagatgtaac
    68941 agcaaactgt cctgcagcaa ccagcattgc cacctcctgt cagagtcgta aaattcgcca
    69001 acgacgatta ttcggcgtta cacttatcac tcatacaaat caaatagcag gattttgcag
    69061 tgttacaact tcttttagca gtttttattg gcggtggtac gggaagcgta gcgagatggc
    69121 tgttaagtat gcgatttaac ccgctgcatc aggcgattcc gttggggacg ctggcagcaa
    69181 acctgattgg ggcattcatc ataggaatgg gattcgcctg gttcagcagg atgacgaaca
    69241 ttgatccagt gtggaaagta ttaatcacca ccggattttg tggcggtcta acaaccttct
    69301 caacattttc ggcagaagtg gtgtttttgt tacaagaggg ccgctttggc tgggcattac
    69361 tgaacgtttt cgtcaacctt ctggggtctt ttgccatgac cgcactggca ttctggctgt
    69421 tttcggcctc aaccgtacac taaagaccaa aaaaaacccg ctgattaagc gggttttgaa
    69481 ttcttgctga cgtatcttac agagcgatta cgtttgcagc agaagggcct ttggcaccgt
    69541 tagtgatttc gaactctacg cgctgacctt cagcaagagt tttaaaacca ttagtctgga
    69601 ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc cggagtaatg aaaccgaatc
    69661 ctttggactc attaaaccac ttaacgttac ctttaatctt agacatcaaa attaccttta
    69721 catgaaaaat agacacaaat gctgtgtcgg ttatcagtac accaattgtg gtacgctttg
    69781 tccagtgcaa ctttactaaa aagtgataaa agtcgcgtta tttttgatgt tgcttatttt
    69841 agttattgat tttaaagaag ttactaaaac ttcatttgtc tcaaaactga aagcgcatcc
    69901 aggcaaagta cacattgcca ttgttgtagg tacccggaat gtaggtcatc tgaaaagtca
    69961 ctgggccata acccacggag gccaatggca gtagaaccgg gagagggatg taattccagt
    70021 tatcgcgtgc cgttacgcca gcggtgaatc ccagacctaa atgaaaattt tcatccgcca
    70081 gcggtcgcca ggtactttcc catccgtatc cggcaatcgg ttcccatttg ttccacgagt
    70141 ccttaaatgc catggcatac aggccatgcc agtttccttt ttcatcccaa cgcgacaggc
    70201 caaaaccgcc accccacggt cgctcgttat agcgatcggt tttttctttg tcgtaagcga
    70261 aacgtgcatg ccaggtgatg gcaggaatat ataaatcata atgttcaggc tgttgccagg
    70321 tttgtgcaat attttctcta aacgttgtca tcctctcatc tgcgttagca aaaactttac
    70381 caacgctgat taactgaata aaaacaaagg aaaagatagc gacatattta ctcacgttca
    70441 tttgtgacca taaaacattt atcaaaaatc tactactagc atagcaaagc tacaattaac
    70501 ataaccttaa tagacccaac ataaagaata atctgaatag ctgctctatt tatttccgct
    70561 tgttcaagtt aaatatttcc gcgcatcgat taaagatgat gcgtcgtttt ctgcgatggg
    70621 aatagtcaaa aaagaaaaac caagtctttt ttgatgacaa aatgcaatca aggaaaaatt
    70681 attttatttt tttaacgaga tacaacaatc atcttaacga agtatataat atatatcgtc
    70741 aacccatgct catattagat tattttttga gcgcacagcc gcagctaaca ccattgcaat
    70801 taacaaattt gcatcaatcc accatcaatt tacacacatt attatgtgat aattgccaac
    70861 cgctaaatat gcgttttgtt atctatgtat aaaaacagca acttcaatgt cttaatggca
    70921 gtttttcttg attttaatca gcattcatcg ccaatttatt gggcatattt tttccttaag
    70981 ctttaggaag tttttattat ttactttggg cctggagaca ggaaaaatta tgctgacatt
    71041 cattgaactc cttattgggg ttgtggttat tgtgggtgtg gctcgctaca tcattaaagg
    71101 gtattccgcc actggtgtgt tatttgtcgg tggcctgtta ttgctgatta tcagtgccat
    71161 tatggggcac aaagtgttac cgtccagcca ggcttcaaca ggctacagcg ccacggatat
    71221 cgttgaatac gttaaaatat tactaatgag ccgcggcggc gacctcggca tgatgattat
    71281 gatgctgtgt ggctttgccg cttacatgac ccatatcggc gcgaatgata tggtggtcaa
    71341 gctggcgtca aaaccattgc agtatattaa ctccccttac ctgctgatga ttgccgccta
    71401 ttttgttgct tgtctgatgt cactggccgt ctcttccgca accggtctgg gggttttgct
    71461 gatggcaacc ctgtttccgg tgatggtaaa cgttggtatc agtcgtggcg cagcagctgc
    71521 catttgtgcc tccccggcgg cgattattct cgcaccgact tcaggggatg tggtgctggc
    71581 ggcgcaggct tccgaaatgt cgctgattga cttcgccttc aaaacgacgc tgcctatctc
    71641 aattgctgca attatcggca tggcgatcgc ccacttcttc tggcaacgtt atctggataa
    71701 aaaagagcac atctctcatg aaatgttaga tgtcagtgaa atcaccacca ctgcccccgc
    71761 gttttatgcc attttgccgt tcacgccgat catcggtgta ctgatttttg acggtaaatg
    71821 gggtccgcaa ttacacatca tcactattct ggtgatttgt atgctgattg cctccattct
    71881 ggagttcctc cgcagcttta atacccagaa agttttctct ggtctggaag tggcttatcg
    71941 cgggatggca gatgcgtttg ctaacgtggt gatgctgctg gttgccgctg gggtattcgc
    72001 tcaggggctt agcaccatcg gctttattca aagtctgatt tctatcgcta cctcgtttgg
    72061 ttcggcgagt atcatcctga tgctggtatt ggtgattctg accatgctgg cggcagtcac
    72121 gaccggttca ggcaatgcgc cgttttatgc gtttgttgag atgatcccga aactggcgca
    72181 ctcttccggc attaacccgg cgtatttgac tattccgatg ctgcaggcgt caaacctggg
    72241 tcgtacccta tcacccgttt ctggcgtagt cgttgcggtt gccgggatgg cgaaaatctc
    72301 accatttgaa gtcgtaaaac gcacctcggt gccggtgctt gttgggctag tgattgttat
    72361 cgttgctaca gagctgatgg tgccaggaac ggcagcagcg gtcacaggca agtaaatagt
    72421 aatgccggag gcaagtttcc tccggcttta tcatgtcacc cactgtggta tatgcgttgt
    72481 ggtctgccaa ctttgccgtg aacaatttca gcaataatca gatggcggct ggcgcaatat
    72541 tcaagataac gcctggcagt ggtgcggctg atggtcagtg cctgcgccac cgtttctgcc
    72601 gtatgttgca caccaggctc tttaaacagt tttcgcaccg cgtttagcgt caaggggtca
    72661 atgccggtcg gcagttcgtc tttgggttca ccgcgagcat aggcattaaa catctcatca
    72721 atttgcttct ggctggcgct atcaatactt tccagcatat gtttacgctg gcggaaacgg
    72781 gttagcgttt gccccagtgt attgattcac ttgaagtacg aaaaaaccgg gagaacattg
    72841 gattattcgg gatctgatgg gattagattt ggtggggctt gcaagcctgt agtacaaatt
    72901 ttaggcgtta accaatgaaa cgcgaaagat agtaaaaaat tgcttttgtt tcattgaaaa
    72961 aacgaaaaac aaaaacacta taaatcattt caatcatagc ttcaaaaaac gttcaaaacc
    73021 gataacaacc aggctgtcat caaatgactc atatcacaaa tcagctcatg ccgtttaggt
    73081 atgttacatg tgtgattatg tgaggtgaag tatgttttag ctggctcatg gttgttatac
    73141 ggcttttttc gcatcttgtg gttccaacaa aggagctaca ggctcttcct gtttatgaac
    73201 accatacatt gacaaaatct cttggcgagt ttctggaggt aatttatgca gcgccaaagc
    73261 tgtttgtagt gtttggcgat caatacccag cgttgccaat agggtgtcaa cggagatgga
    73321 ggggcgttct tgcattaatc cggaaagccc atatttagcc atgtttagct ctatctctcg
    73381 gacttcattt tctcctagtt tattgattaa cagggagaac attttcaatg tatcagcatt
    73441 catttcttcg atagcaattt tttgcatagc cggtattgcc gggatatatt caaccccagg
    73501 cccgcgacgc ccttcttgtg aacgcttcgg ccatccttct ttctttgctc gtttatgcac
    73561 cccctgtatt gtcccaggca ttccatccag ttcagtaatt tgtttgggtg tcatccactg
    73621 gtttatctca tttgcgcttt tcataaacta agtttaccag atttatctat ataaaaatca
    73681 atggattaca aaataaacca cattaactga tataaaccgg tttggtttat ttggtaatgt
    73741 ataaaacaga taatcaagac gaattatttg ttttggtaac cctttatcat acgcaatatg
    73801 gttggtgtgc gaaagatgag taacgattgg catcgggccg acgtgattgc ggccctcaaa
    73861 aaaacaggaa cccctctggc aaaattgtcc agacgttcag gttattcaac cgggacgctt
    73921 gcaaatgcat tagtcaggcc atggcctaaa ggtgaacgca ttattgctga cgccttaaac
    73981 atgaaacctg aggaaatttg gcctagtaga tatctaaggg atcacaatga gagagtggat
    74041 atcagcaatt gaatgcatgg gtttatctgg tttacctggt tcattaatgg gggttcacaa
    74101 aagagctcag agagaaggat ggctcaagag aagaagagaa ggaattagag gggttgctta
    74161 cgaataccat atatcttctc ttcctaacat tgctcgagct gaagttttgc tccaacaagg
    74221 aaaatttaaa accagtcagg gatgttttga aatcgcccgc cccacgctgg aagctcatga
    74281 ttatgatcgt gaggcactat ggagcaaatg ggataaagcc agcgattccc agcgcagcct
    74341 ggctgaaaaa tggttgccct caattcaggc cacagacgaa atgctgaacc aggggatttc
    74401 aacgaaaacg gcttttgcga ccgttgcagg gcattaccag gtcagcgcat ccactttgcg
    74461 ggacaagtat taccaggtac agaagtttgc gaagcctgac tgggcggctg cacttgttga
    74521 tggacgtgga gcatcccgtc gcaatgttca caaaagtgaa tttgacgagg atgcctggca
    74581 gtttctgatt gcagattatc tgcgaccgga aaaacccgcc ttccgcaaat gttatgagcg
    74641 tctggaactg gcagcccgcg agcatggctg gagtattccc tcccgtgcca cggcctttcg
    74701 ccggattcag caactggacg aggcaatggt tgttgcctgt cgtgaaggtg aacatgcact
    74761 gatgcatctg ataccggcac agcagcgaac tgtggaacac ctggacgcca tgcagtggat
    74821 caacggcgac ggttatctgc ataacgtctt tgtacgctgg tttaacggtg atgtgatccg
    74881 tccgaaaaca tggttctggc aggatgtgaa aacccgaaaa attctgggct ggcgctgcga
    74941 tgtgagcgag aacattgatt caattcgcct ctcgttcatg gatgttgtga ctcgctacag
    75001 tatcccggag gattttcaca tcaccattga taacacccgt ggtgctgcga ataaatggct
    75061 gacgggaggc gcgcccaatc gctaccgctt taaggtaaaa gaggacgatc caaaaggact
    75121 gtttttactg atgggtgcga aaatgcactg gacaagcgtt gttgccggta aaggctgggg
    75181 ccaggcaaaa cctgttgaac gtgctttcgg tgttggtggg cttgaggaat acgttgataa
    75241 gcatccggca ctggctggcg catatacggg gccaaatccg caggcaaaac ctgataacta
    75301 tggcgaccac gctgttgatg cagagctgtt tctgaaaacc tttgccgaag gtgtggcgat
    75361 gttcaatgcc agaacaggcc gtgaaacaga aatgtgcggg ggcaaactct cgtttgatga
    75421 tgttttcgag cgtgaatacg ccagaacgat tgtgcgtaag ccaaccgaag aacaaaaacg
    75481 gatgctgtta ctgcctgccg aggcggtgaa cgtttcacgc aaaggcgagt ttacgcttaa
    75541 agttggcggc tcccttaaag gcgcgaaaaa cgtttattac aacatggcat taatgaatgc
    75601 cggagtgaaa aaagttgtgg tcagatttga tccacagcag ttacacagca cggtttattg
    75661 ag
//
LOCUS       DAJTRH010000004        72933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTRH010000004 DAJTRH010000000
VERSION     DAJTRH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO339_000315"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO339_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5925072.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO339_000316"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO339_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5925073.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO339_000317"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO339_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5925074.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO339_000318"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO339_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5925075.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO339_000319"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO339_000319"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5925076.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO339_000320"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO339_000320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5925077.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO339_000321"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO339_000321"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5925078.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO339_000322"
     CDS             complement(4598..5320)
                     /locus_tag="OO339_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5925079.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO339_000323"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO339_000323"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5925080.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO339_000324"
     CDS             6520..6738
                     /locus_tag="OO339_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5925081.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO339_000325"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO339_000325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5925082.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO339_000326"
     CDS             complement(7472..7672)
                     /locus_tag="OO339_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5925083.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO339_000327"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO339_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO339_000328"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO339_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5925084.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO339_000329"
     CDS             10101..12014
                     /locus_tag="OO339_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5925085.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO339_000330"
     CDS             12014..13036
                     /locus_tag="OO339_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5925086.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO339_000331"
     CDS             13030..13248
                     /locus_tag="OO339_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5925087.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO339_000332"
     CDS             13302..14171
                     /locus_tag="OO339_000332"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5925088.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO339_000333"
     CDS             complement(14226..14630)
                     /locus_tag="OO339_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5925089.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO339_000334"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO339_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5925090.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO339_000335"
     CDS             15615..17705
                     /locus_tag="OO339_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5925091.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO339_000336"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO339_000336"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5925092.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO339_000337"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO339_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5925093.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO339_000338"
     CDS             complement(19673..20275)
                     /locus_tag="OO339_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5925094.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO339_000339"
     CDS             complement(20265..20432)
                     /locus_tag="OO339_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5925095.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO339_000340"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO339_000340"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5925096.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO339_000341"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO339_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5925097.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO339_000342"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO339_000342"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5925098.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO339_000343"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO339_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5925099.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO339_000344"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO339_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5925100.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO339_000345"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO339_000345"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5925101.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO339_000346"
     CDS             28269..28436
                     /locus_tag="OO339_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5925102.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO339_000347"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO339_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO339_000348"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO339_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5925103.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO339_000349"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO339_000349"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5925104.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO339_000350"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO339_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5925105.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO339_000351"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO339_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5925106.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO339_000352"
     CDS             complement(33764..34849)
                     /locus_tag="OO339_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5925107.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO339_000353"
     CDS             complement(34861..36165)
                     /locus_tag="OO339_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5925108.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO339_000354"
     CDS             complement(36177..36530)
                     /locus_tag="OO339_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5925109.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO339_000355"
     CDS             complement(36541..37419)
                     /locus_tag="OO339_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5925110.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO339_000356"
     CDS             complement(37416..38642)
                     /locus_tag="OO339_000356"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5925111.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDNCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO339_000357"
     CDS             complement(38643..39806)
                     /locus_tag="OO339_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5925112.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO339_000358"
     CDS             complement(39890..40252)
                     /locus_tag="OO339_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5925113.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO339_000359"
     CDS             complement(40269..41174)
                     /locus_tag="OO339_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925114.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO339_000360"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO339_000360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5925115.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO339_000361"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO339_000361"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5925116.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO339_000362"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO339_000362"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5925117.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO339_000363"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO339_000363"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5925118.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO339_000364"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO339_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5925119.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO339_000365"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO339_000365"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5925120.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO339_000366"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO339_000366"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5925121.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO339_000367"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO339_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5925122.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO339_000368"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO339_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO339_000369"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO339_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5925123.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO339_000370"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO339_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5925124.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO339_000371"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO339_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5925125.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="OO339_000372"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="OO339_000372"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5925126.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO339_000373"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO339_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5925127.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="OO339_000374"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="OO339_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5925128.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="OO339_000375"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="OO339_000375"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5925129.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="OO339_000376"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="OO339_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5925130.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="OO339_000377"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="OO339_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5925131.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61355)
                     /locus_tag="OO339_000378"
     CDS             complement(59604..61355)
                     /locus_tag="OO339_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5925132.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61734..63356
                     /gene="pckA"
                     /locus_tag="OO339_000379"
     CDS             61734..63356
                     /gene="pckA"
                     /locus_tag="OO339_000379"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5925133.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO339_000380"
     CDS             complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO339_000380"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5925134.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO339_000381"
     CDS             complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO339_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5925135.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65728..66204
                     /gene="greB"
                     /locus_tag="OO339_000382"
     CDS             65728..66204
                     /gene="greB"
                     /locus_tag="OO339_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5925136.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66301..68622
                     /locus_tag="OO339_000383"
     CDS             66301..68622
                     /locus_tag="OO339_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5925137.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69060..69287
                     /gene="feoA"
                     /locus_tag="OO339_000384"
     CDS             69060..69287
                     /gene="feoA"
                     /locus_tag="OO339_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5925138.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69304..71625
                     /gene="feoB"
                     /locus_tag="OO339_000385"
     CDS             69304..71625
                     /gene="feoB"
                     /locus_tag="OO339_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5925139.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71625..71861
                     /gene="feoC"
                     /locus_tag="OO339_000386"
     CDS             71625..71861
                     /gene="feoC"
                     /locus_tag="OO339_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5925140.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72064..>72933
                     /gene="rpnA"
                     /locus_tag="OO339_000387"
     CDS             72064..>72933
                     /gene="rpnA"
                     /locus_tag="OO339_000387"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5925141.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAA"
BASE COUNT        17577 a        19761 c        18447 g        17148 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgctccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa ttatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccctgaagta atccggtagc aatcatcccc
    61321 cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa tcaaaaggtg
    61381 ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa tcaaaaaaag
    61441 ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt caggaatgcg
    61501 attccactca caatattccc gccatataaa ccaagattta accttttgag aacattttcc
    61561 acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa tcacggagtt
    61621 ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg cggttaacac
    61681 ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt gaaatgcgcg
    61741 ttaacaatgg tttgcccccg caagaactcg aggcttatgg tatcagtgac gtacatgata
    61801 tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg agcctgacag
    61861 gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc gggatcttca
    61921 ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc gatactttct
    61981 ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg gaaacctggc
    62041 agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc gttgtcgacg
    62101 ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc gaagtggcct
    62161 ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag ctggcaggtt
    62221 tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag tggaaagaac
    62281 agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg cagctgattg
    62341 gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg aactacctgc
    62401 tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag aaaggcgatg
    62461 ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc gacccgaaac
    62521 gccgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt aatttcgaag
    62581 gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa atctacaacg
    62641 ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc actatcgact
    62701 ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccaatctat cacatcgata
    62761 acatcgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc ctgactgctg
    62821 atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc cagtatcact
    62881 tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc gaaccgacgc
    62941 caaccttctc cgcttgcttc ggcgcggcgt tcctgtcgct gcacccgact cagtacgcag
    63001 aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt aacactggct
    63061 ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc gacgccatcc
    63121 tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac ctggcgatcc
    63181 caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc tacgcttctc
    63241 cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac aacttcgata
    63301 aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa ctgtaatgat
    63361 ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag gcaccttcgc
    63421 ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg cactggcagc
    63481 caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat cccgttatgg
    63541 ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat ggtgcgcgca
    63601 ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga
    63661 ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc
    63721 cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg tttgatcgac
    63781 agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca
    63841 tagccacttt cggcagcaat cacctcaccg agcactgcat taagatccgc catttccatc
    63901 ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct
    63961 tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat catctcagtc
    64021 gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc catcagcagc
    64081 gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc acgggtaacg
    64141 gaacgcacct ccgaagcgcc atattcacgc agcggcggcg gaataatccc tttaccaacc
    64201 tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat aaacagccac
    64261 gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg agagaaatcg
    64321 ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag ccaggttttc
    64381 agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg gccgcccagt
    64441 tgctgtgcca tctgatggct taagaattca tagtgttgcg cccaacgcag acctgcctct
    64501 tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa
    64561 gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt catcaacata
    64621 cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc
    64681 agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat gagcaataac
    64741 gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gagccgtccg
    64801 gtacaaagac gtagcccagg ccccaaacgg tctgaatgta acgcggatgc gctggatctt
    64861 cttctaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggaat
    64921 attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca cgcggatggc
    64981 tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc
    65041 ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt
    65101 cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca cgaatacggg
    65161 ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc
    65221 ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc
    65281 tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac attaaatcca
    65341 gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga
    65401 cgcttcgaac ctggaagcct tgttcggtga gataacgttc cagcagcgca cgcaggcgca
    65461 tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt
    65521 cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg
    65581 gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt
    65641 tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt
    65701 atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat
    65761 gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa
    65821 aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat
    65881 aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg cctggaaaat
    65941 ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tcttttttgg cgcgtgggtg
    66001 gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt
    66061 tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt gctgaaaaaa
    66121 gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgaactg gtatgttaat
    66181 gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt
    66241 ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc
    66301 atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag
    66361 gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat
    66421 cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg
    66481 agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa
    66541 ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa aaccgaactc
    66601 gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa
    66661 gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac gccagaagtc
    66721 gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc cgcgctggat
    66781 ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccacgctgct ggcgaaagtg
    66841 cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg taaagaagag
    66901 gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac ggtgccttct
    66961 caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct ttcgctgaat
    67021 gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat catcatggat
    67081 caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaaag cgtagtgagc
    67141 tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg taccgtgcgc
    67201 gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg
    67261 gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta
    67321 aaagtggcgg tggtcgatgc cactggcaaa ctggtggcga ccgataccat ttacccgcac
    67381 accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa acataacgtt
    67441 gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt ctatctcgac
    67501 gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga agctggcgcg
    67561 tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg
    67621 cgtggtgcgg tatctatcgc ccgccgtttg caggatccgc tggcggagct ggtgaaaatc
    67681 gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcaactggcc
    67741 cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga tctcaacacc
    67801 gcttctgttc cgctattaac ccgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc
    67861 gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct gaaagtcagc
    67921 cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc tgcgcattaa ccacggtgat
    67981 aacccgctgg acgcctctac cgttcatccg gaagcctatc cggtggtgga acgcattctg
    68041 gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact gcgtaacctg
    68101 aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga catcatcaaa
    68161 gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca gtttgccgat
    68221 ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc agtgaccaac
    68281 gtcaccaact ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct ggttcacatc
    68341 tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt
    68401 gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct gactatgcgt
    68461 ctggacgagc agcctggcga aaccaactct cgtcgcggcg gcggtaatga acgcccgcaa
    68521 aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg taatagcgcg
    68581 atgatggatg cgctggcggc agcgatgggt aaaaaacgtt aaacgcccgg actggcccct
    68641 aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgctttgc ctgacataaa
    68701 agtgccggag aatatctccg gcacttttat tccacagcca aactcacaat atactccgtt
    68761 aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag tccgggtaac
    68821 tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa cattgagtca
    68881 gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga aaccattctc
    68941 attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat ctggtctcat
    69001 gtcgcggtca aacgccccat gaggtagtta tccagttaat gagaaacaag taggcaccta
    69061 tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa atcagcccgg
    69121 catatcgcca aaaactgctt tctctaggca tgttacctgg ctcctctttt gatgtggtgc
    69181 gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc ctggtattac
    69241 gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac ggtgataaca
    69301 acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa gacaacctta
    69361 tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt taccgtcgaa
    69421 cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga cctgcccggc
    69481 acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat cgcctgtcac
    69541 tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc taaccttgag
    69601 cgtaacctgt acctgacgct gcaactgctg gaactcggca ttccctgcat cgtggcgctg
    69661 aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc tctatcggca
    69721 cgtcttggct gcccggtgat cccgctggtt tcaacccgtg gtcgcggtat tgaagcgctc
    69781 aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca ttacgcacag
    69841 ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat cccgctgaaa
    69901 caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg cgcctacgcc
    69961 ggtgaagcgt cgcagcatct ggatgccacc ctcgcccgtc tgcgtaatga aatggatgat
    70021 ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg tgatgtggta
    70081 agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa aatcgtgctc
    70141 aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt cctgctggct
    70201 atcaacatcg gcggggcgtt acagccgctg tttaacgtcg gctccgtggc gctatttgtg
    70261 catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac tatcttcctc
    70321 gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca gatcggcatg
    70381 atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc agcgtttgtg
    70441 atggaccgcc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc gctgatcgtc
    70501 ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc accgcgtgaa
    70561 cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct ggctatcttc
    70621 gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt ctcgctgtat
    70681 atgctcggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac catcatgcgc
    70741 ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca cgttaaaagc
    70801 ctgattatcc agacctggca gcgtctgaag ggcttcgttc tgcgtgctgg taaagtgatt
    70861 atcatcgtca gcattttcct gagcgctttc aacagcttct cgttgagcgg gaaaatcgtc
    70921 gataacatca acgactcggc gctggcttcc gtcagccggg tgataacccc tgtcttcaag
    70981 ccgattggcg tacatgaaga taactggcag gcaacggttg gcctgtttac gggcgcaatg
    71041 gcaaaagaag tggtggtggg tacgctcaac accctctaca ccgcagaaaa tattcaggac
    71101 gaagagttca atccggcgga attcaacctc ggtgaagagc tgttcagtgc ggtagatgaa
    71161 acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc cattgaagct
    71221 agcaaaggca acggtgaaat gggtaccggg gcgatgggcg tgatggatca gaaattcggt
    71281 agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc atgtatctcg
    71341 gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc catcctgtgg
    71401 gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag ctacagtcag
    71461 cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat cgtagctatc
    71521 ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac ccgcaagtcg
    71581 gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct tcacttattc
    71641 aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata agccagacat
    71701 tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc atgggcaaag
    71761 ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa agctgcccgg
    71821 aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc atcgcctgtt
    71881 ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt ccataaaaat
    71941 gcaattcttg cagcgcaacg taagttcctc tgaaagcgtc tcgcagggat gaaaactcgc
    72001 taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag gtgaaggaac
    72061 gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact ctttttacgc
    72121 caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat tcacgcgctc
    72181 tgtgatatga aaaccctcaa gctggagtcg aggagcttta ttgatgacga tctgcgtgaa
    72241 agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata catctattgt
    72301 ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat gcgttacgct
    72361 attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat ggtggtgcca
    72421 ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg gctggattgt
    72481 ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct gattgatgtc
    72541 accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct ggagttaatt
    72601 caaaaacata ttcgtcaacg cgatctgatg gggctggtag agcaaatggc ctgcttatta
    72661 agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat actgcaaact
    72721 ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc accgaaacac
    72781 aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca atccaaagcc
    72841 ctgcatatag ccaaaataat gcttgaatcc ggagtccctc ttgcagacat catgcgattt
    72901 accgggctgt cagaagaaga gttggctgcg gcg
//
LOCUS       DAJTRH010000005        71489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTRH010000005 DAJTRH010000000
VERSION     DAJTRH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(764..2350)
                     /locus_tag="OO339_000388"
     CDS             complement(764..2350)
                     /locus_tag="OO339_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5925142.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2705..4876)
                     /gene="glcB"
                     /locus_tag="OO339_000389"
     CDS             complement(2705..4876)
                     /gene="glcB"
                     /locus_tag="OO339_000389"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5925143.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4898..5302)
                     /locus_tag="OO339_000390"
     CDS             complement(4898..5302)
                     /locus_tag="OO339_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5925144.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5307..6526)
                     /gene="glcF"
                     /locus_tag="OO339_000391"
                     /pseudo
     CDS             complement(5307..6526)
                     /gene="glcF"
                     /locus_tag="OO339_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6537..7589)
                     /gene="glcE"
                     /locus_tag="OO339_000392"
     CDS             complement(6537..7589)
                     /gene="glcE"
                     /locus_tag="OO339_000392"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5925145.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7589..9088)
                     /gene="glcD"
                     /locus_tag="OO339_000393"
                     /pseudo
     CDS             complement(7589..9088)
                     /gene="glcD"
                     /locus_tag="OO339_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9339..10109
                     /gene="glcC"
                     /locus_tag="OO339_000394"
     CDS             9339..10109
                     /gene="glcC"
                     /locus_tag="OO339_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5925146.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10233..11374)
                     /locus_tag="OO339_000395"
                     /pseudo
     CDS             complement(10233..11374)
                     /locus_tag="OO339_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11736..13466
                     /locus_tag="OO339_000396"
     CDS             11736..13466
                     /locus_tag="OO339_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5925147.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13463..14377
                     /locus_tag="OO339_000397"
     CDS             13463..14377
                     /locus_tag="OO339_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5925148.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14410..14658
                     /locus_tag="OO339_000398"
     CDS             14410..14658
                     /locus_tag="OO339_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5925149.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14658..15829
                     /locus_tag="OO339_000399"
                     /pseudo
     CDS             14658..15829
                     /locus_tag="OO339_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15865..16944)
                     /gene="lptG"
                     /locus_tag="OO339_000400"
     CDS             complement(15865..16944)
                     /gene="lptG"
                     /locus_tag="OO339_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5925150.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16941..18011)
                     /gene="lptF"
                     /locus_tag="OO339_000401"
     CDS             complement(16941..18011)
                     /gene="lptF"
                     /locus_tag="OO339_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5925151.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18042..18602)
                     /locus_tag="OO339_000402"
     CDS             complement(18042..18602)
                     /locus_tag="OO339_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5925152.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18614..19438)
                     /locus_tag="OO339_000403"
     CDS             complement(18614..19438)
                     /locus_tag="OO339_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925153.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19438..20787)
                     /locus_tag="OO339_000404"
     CDS             complement(19438..20787)
                     /locus_tag="OO339_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5925154.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20833..21591)
                     /locus_tag="OO339_000405"
     CDS             complement(20833..21591)
                     /locus_tag="OO339_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925155.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21623..22336)
                     /locus_tag="OO339_000406"
     CDS             complement(21623..22336)
                     /locus_tag="OO339_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5925156.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22510..23202
                     /locus_tag="OO339_000407"
     CDS             22510..23202
                     /locus_tag="OO339_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5925157.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23251..24750)
                     /gene="pitB"
                     /locus_tag="OO339_000408"
     CDS             complement(23251..24750)
                     /gene="pitB"
                     /locus_tag="OO339_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5925158.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25042..26901)
                     /gene="gss"
                     /locus_tag="OO339_000409"
     CDS             complement(25042..26901)
                     /gene="gss"
                     /locus_tag="OO339_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5925159.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27106..27972
                     /gene="yghU"
                     /locus_tag="OO339_000410"
     CDS             27106..27972
                     /gene="yghU"
                     /locus_tag="OO339_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5925160.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28095..28343)
                     /gene="hybG"
                     /locus_tag="OO339_000411"
     CDS             complement(28095..28343)
                     /gene="hybG"
                     /locus_tag="OO339_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5925161.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28477..28818)
                     /gene="hypA"
                     /locus_tag="OO339_000412"
     CDS             complement(28477..28818)
                     /gene="hypA"
                     /locus_tag="OO339_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5925162.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28811..29299)
                     /gene="hybE"
                     /locus_tag="OO339_000413"
     CDS             complement(28811..29299)
                     /gene="hybE"
                     /locus_tag="OO339_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5925163.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29292..29786)
                     /locus_tag="OO339_000414"
     CDS             complement(29292..29786)
                     /locus_tag="OO339_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5925164.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29786..31489)
                     /gene="hybC"
                     /locus_tag="OO339_000415"
     CDS             complement(29786..31489)
                     /gene="hybC"
                     /locus_tag="OO339_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5925165.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31486..32664)
                     /gene="hybB"
                     /locus_tag="OO339_000416"
     CDS             complement(31486..32664)
                     /gene="hybB"
                     /locus_tag="OO339_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5925166.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32654..33640)
                     /gene="hybA"
                     /locus_tag="OO339_000417"
     CDS             complement(32654..33640)
                     /gene="hybA"
                     /locus_tag="OO339_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5925167.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33643..34761)
                     /gene="hybO"
                     /locus_tag="OO339_000418"
     CDS             complement(33643..34761)
                     /gene="hybO"
                     /locus_tag="OO339_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5925168.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34950..35237)
                     /locus_tag="OO339_000419"
     CDS             complement(34950..35237)
                     /locus_tag="OO339_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5925169.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35356..36243)
                     /locus_tag="OO339_000420"
     CDS             complement(35356..36243)
                     /locus_tag="OO339_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5925170.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36400..37440
                     /gene="gpr"
                     /locus_tag="OO339_000421"
     CDS             36400..37440
                     /gene="gpr"
                     /locus_tag="OO339_000421"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5925171.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37480..37974)
                     /locus_tag="OO339_000422"
     CDS             complement(37480..37974)
                     /locus_tag="OO339_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5925172.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38165..39049
                     /gene="yghA"
                     /locus_tag="OO339_000423"
     CDS             38165..39049
                     /gene="yghA"
                     /locus_tag="OO339_000423"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5925173.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39321..39746)
                     /gene="exbD"
                     /locus_tag="OO339_000424"
     CDS             complement(39321..39746)
                     /gene="exbD"
                     /locus_tag="OO339_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5925174.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39753..40487)
                     /gene="exbB"
                     /locus_tag="OO339_000425"
     CDS             complement(39753..40487)
                     /gene="exbB"
                     /locus_tag="OO339_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5925175.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40739..41926
                     /gene="metC"
                     /locus_tag="OO339_000426"
     CDS             40739..41926
                     /gene="metC"
                     /locus_tag="OO339_000426"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5925176.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42066..42725
                     /locus_tag="OO339_000427"
     CDS             42066..42725
                     /locus_tag="OO339_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5925177.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42765..43664)
                     /gene="yqhC"
                     /locus_tag="OO339_000428"
     CDS             complement(42765..43664)
                     /gene="yqhC"
                     /locus_tag="OO339_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5925178.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43858..45021
                     /gene="yqhD"
                     /locus_tag="OO339_000429"
     CDS             43858..45021
                     /gene="yqhD"
                     /locus_tag="OO339_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5925179.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45126..45953
                     /gene="dkgA"
                     /locus_tag="OO339_000430"
     CDS             45126..45953
                     /gene="dkgA"
                     /locus_tag="OO339_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5925180.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46153..47079
                     /locus_tag="OO339_000431"
     CDS             46153..47079
                     /locus_tag="OO339_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5925181.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47130..47387
                     /gene="yqhH"
                     /locus_tag="OO339_000432"
     CDS             47130..47387
                     /gene="yqhH"
                     /locus_tag="OO339_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5925182.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47430..49649)
                     /locus_tag="OO339_000433"
     CDS             complement(47430..49649)
                     /locus_tag="OO339_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5925183.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49760..51172)
                     /gene="ftsP"
                     /locus_tag="OO339_000434"
     CDS             complement(49760..51172)
                     /gene="ftsP"
                     /locus_tag="OO339_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5925184.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51247..51984)
                     /gene="plsC"
                     /locus_tag="OO339_000435"
                     /gene_synonym="parF"
     CDS             complement(51247..51984)
                     /gene="plsC"
                     /locus_tag="OO339_000435"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5925185.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52218..54476)
                     /gene="parC"
                     /locus_tag="OO339_000436"
     CDS             complement(52218..54476)
                     /gene="parC"
                     /locus_tag="OO339_000436"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5925186.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55022..55504)
                     /locus_tag="OO339_000437"
     CDS             complement(55022..55504)
                     /locus_tag="OO339_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5925187.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55557..55949)
                     /gene="ygiW"
                     /locus_tag="OO339_000438"
     CDS             complement(55557..55949)
                     /gene="ygiW"
                     /locus_tag="OO339_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5925188.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56101..56760
                     /gene="qseB"
                     /locus_tag="OO339_000439"
     CDS             56101..56760
                     /gene="qseB"
                     /locus_tag="OO339_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5925189.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56757..58031
                     /gene="qseC"
                     /locus_tag="OO339_000440"
     CDS             56757..58031
                     /gene="qseC"
                     /locus_tag="OO339_000440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5925190.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58077..58409)
                     /locus_tag="OO339_000441"
     CDS             complement(58077..58409)
                     /locus_tag="OO339_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5925191.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58728..59309
                     /gene="mdaB"
                     /locus_tag="OO339_000442"
     CDS             58728..59309
                     /gene="mdaB"
                     /locus_tag="OO339_000442"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5925192.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59340..59654
                     /locus_tag="OO339_000443"
     CDS             59340..59654
                     /locus_tag="OO339_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5925193.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59702..61594)
                     /gene="parE"
                     /locus_tag="OO339_000444"
     CDS             complement(59702..61594)
                     /gene="parE"
                     /locus_tag="OO339_000444"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5925194.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61623..62204)
                     /gene="yqiA"
                     /locus_tag="OO339_000445"
     CDS             complement(61623..62204)
                     /gene="yqiA"
                     /locus_tag="OO339_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5925195.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62204..63031)
                     /gene="cpdA"
                     /locus_tag="OO339_000446"
     CDS             complement(62204..63031)
                     /gene="cpdA"
                     /locus_tag="OO339_000446"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5925196.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63056..63478)
                     /locus_tag="OO339_000447"
     CDS             complement(63056..63478)
                     /locus_tag="OO339_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5925197.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63479..64108)
                     /gene="nudF"
                     /locus_tag="OO339_000448"
     CDS             complement(63479..64108)
                     /gene="nudF"
                     /locus_tag="OO339_000448"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5925198.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64313..65794
                     /gene="tolC"
                     /locus_tag="OO339_000449"
     CDS             64313..65794
                     /gene="tolC"
                     /locus_tag="OO339_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5925199.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65942..66613
                     /locus_tag="OO339_000450"
     CDS             65942..66613
                     /locus_tag="OO339_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5925200.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66619..67779
                     /locus_tag="OO339_000451"
     CDS             66619..67779
                     /locus_tag="OO339_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5925201.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDTGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67817..68605)
                     /gene="ygiD"
                     /locus_tag="OO339_000452"
     CDS             complement(67817..68605)
                     /gene="ygiD"
                     /locus_tag="OO339_000452"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5925202.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68748..69521
                     /gene="zupT"
                     /locus_tag="OO339_000453"
     CDS             68748..69521
                     /gene="zupT"
                     /locus_tag="OO339_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5925203.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69579..69749)
                     /locus_tag="OO339_000454"
     CDS             complement(69579..69749)
                     /locus_tag="OO339_000454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925204.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREEAAEKVE"
     gene            complement(70010..70663)
                     /gene="ribB"
                     /locus_tag="OO339_000455"
     CDS             complement(70010..70663)
                     /gene="ribB"
                     /locus_tag="OO339_000455"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5925205.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70767..70915)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71037..71327
                     /gene="ubiK"
                     /locus_tag="OO339_000456"
     CDS             71037..71327
                     /gene="ubiK"
                     /locus_tag="OO339_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5925206.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPEPETLPPTL"
BASE COUNT        17838 a        19054 c        17653 g        16944 t
ORIGIN      
        1 ttttaatgat tttttcattt attcacacct aataaaagta ttcaaattca gatgattttt
       61 ctgctcaaca gacacaaaca ggcaataata ctctccaaag cgaacaatat ttggcattac
      121 gatttaatgc ttcataatca ccaaattatt taaccataaa ttttcttatt tctaacaaaa
      181 tgattggtgt gttgttggag ttaaaataca tacataatat taacatctgc caacatttca
      241 ctttccgcaa gacggatgat ttcgaatcct catccacgtt accctatccc aaatatcttt
      301 attaataggc agtaacgaac actgttatag caagattaac ttattaacct aacggcatct
      361 tttatgtaac aagtggagat aaattgctta aaattgatcc tcgacaaatc ttaaaagttc
      421 tcattattct gcaaaatcag ctttgcgaat aatcttatgt ttttgggagt tttgggaatt
      481 ttgatctact cattgttcta gtgaaaaata taaataagag aaaaaacgcc attcgtcatg
      541 aaagcaacag atgaagcaat gtaataacat actaaaattg atctcatgaa agtgggtact
      601 ttataaccag cacagaagcc aagttgtata aatattaaac aattcgcatc gtaaaacgga
      661 gactacgtat aaagaagcca atcgtaaaaa cggttccctg gcgagataaa cgacagacaa
      721 atggaaagcc gggtgatatt taccttcatc cggctttatt gatttacgag actaacatcc
      781 cggtaaacac atacgcctgc agcagggtga taacgctggc aaaaatcaga ctgtgcttca
      841 cggtgtagcg gaacagttca gattctcggc ccaccatgcc cgtcgcggcg caggccacgg
      901 cgatagattg cggggagatc atcttgccag ttacgccgcc gctggtattt gctgccacca
      961 gcagagtgtc agagacgttg atttgctgcg ccgtggtcga ttgcagtgaa ccaaacaggg
     1021 cgtttgaaga agtgtccgac ccggtaagga atacgcccag ccagccgaga aacggtgaga
     1081 aaaacgggaa catcacgcct gtacctgcca gtaccagcgc cagcgtggtg gacatgccgg
     1141 aatagttggt gacgaaggcg aatgccagca ccatgccaat cgacaatatc ggccacttca
     1201 gactaattag catttcggca aagacgccaa tccctttctt gatgcccacg ccgaggatga
     1261 agatagagat aatcgccgca ataaaaatag cggtgccgcc agccgagaga gggtcgaatt
     1321 taaacaccgc atccattggc gttggttggg cgacaatggg tgccgctttc aacacttgtt
     1381 gatgcaaatg agggatctgg aaattaatca ccagtgaata aaacgcgccg cccggagcaa
     1441 ataacgcttt aaacggcttc atggtccaga tggtgaccag caccgttaag attaaaaacg
     1501 gtgaccacgc acgaatgatt tgccccagac tatattctga aggcacggga ccgccagaag
     1561 atggcttatt taccaccatc gcacctgcgg attgccccat gctgattgcc gtttcggtat
     1621 ttttcggccg ccagacttta aggaataaag cgagtgagac gatactcacc agcgccgaag
     1681 taatatccgg cagttccgga ccaatatagt tagaggtaaa gaactgagtg acagcgaagc
     1741 ttcccccagc aaccagcgcc gctggccacg tctctttcac ccctttccag ccgtccatca
     1801 ttgctaccag ccagaacggc acaagaaccg acaggaacgg taactgacgt cccgccattg
     1861 cgccaatgtg gaacggatcg attcccgtta cctgaccggc gaccagaatc ggcacgccca
     1921 acgcaccaaa cgccaccgtc gcaccaaagc cagcagctcc ttccagcaac gcaccaaagg
     1981 agaaaccaat cagtaacacc tgcaaacgct gatcgtcggt gatggagata accgagctgc
     2041 ggataatatc gaactgcccg ctggcaacgg ttaatttata caggaacacc gccgcgacaa
     2101 taatccacgc aattggccat aatccataaa taaagccata gcccgcagca gcaaatgcca
     2161 tatcaatcgg cattttaaag gcgaatatcg caatcaggat agataatata agggttattg
     2221 ctccagcaac atgtcctttc agacgtaata ccgcgagtgc aacgaagaag aatattatcg
     2281 ggatcagggc gaccagagcg gatagcccca gtcctcccat cggcatatac atttgggtcc
     2341 aggtaaccat attgttcagt ctcttattat ctttatatgc ttgatatact taaggttgta
     2401 ataagcaaaa gaggactgaa ctgtaaaata taggcgttat actttacagc aacagtacgc
     2461 cgctaacgca attgctacct ctggcataac aagtatatcg ggtaagggtt tctgttccgc
     2521 acacgcaggc gcagagtatc gttaagatgt ccatattgtt gttttaggcc cgctagtaat
     2581 gcgctacggg tatttaatat tgttaaaccc tgataatcgc tccggttatt tccgggataa
     2641 atgtactacc gcagttacta tcatagcccc gacaataaaa cttgccgggg cttttttgac
     2701 gctattaatg actttctttt tcgcgtaaac gccaggcgtg taataacggt tcggtataac
     2761 cgtttggctg tttcacgccg aggaagatta aatcgctggc agctttaaaa gcacacgagt
     2821 tagcgaaatt ccccgccatc ggacgataag ccggatcgcc agcattttgc tgatcaacca
     2881 ctttcgccat attctccagc gacgcctgca cctgttcttt ggtcagaata ccgtgacgta
     2941 accagttggc gatatgctgg ctggagatac gcagcgttgc gcggtcttcc atcagcgcca
     3001 cgttgtgaat atccggcact tttgaacaac caatcccctg ctccacccaa cgcaccacgt
     3061 accccagaat cccctgcacg ttgttatcca gctcttgttg gatctcttgc gccgaccagt
     3121 tagcgttttc agccaccgga atagtcagca gatcgtccag cagcggttca aattcagcac
     3181 tgaactcggt ctgggcaatg ttggcttgca cgctctgtac gttggtatgg tggtagtgca
     3241 gcgcatggag cgtagcagcg gttggtgacg gaacccaggc tgtgtttgcc ccggcacgca
     3301 gttggtcgcc cttctggctg tacatgtctg ccatcaggtc cggcattgcc cacatgcctt
     3361 taccgatttg cgctttaccg cgcagcccac agaacagacc ggaaagcacg ttattacgtt
     3421 cgtaggcttt gatccacggc gtcgatttca tctggttttt acgcagcatc gggccagctt
     3481 ccatcaccga atgcatttca tcgccggtac ggtcgaggaa accggtattg atgaacgcca
     3541 cgcggttgcg cgcctgagcg atacagctac gcaagttcag cgaggtccgg cgttcttcat
     3601 ccataatgcc cattttcagg gtattcggtg ccataccgag cattgtctca atgcgggtaa
     3661 acagtttgtt ggcgaacgcc acttcctgcg gaccgtgcat tttcggtttc acaatataga
     3721 cgctgccagt gcgcgagttt ttctgcactt ttaaatcata gagggcaatc gcgccagtca
     3781 tgacgccatc aagaatacct tccgggattt cattgccttc gctgtcccaa atcacaggaa
     3841 tggtcatcaa atgacccacg ttgcggataa acagcaacga gcgtccgtgc agagaaattt
     3901 cagagccatc ggcggcggtg tactgacgat cgtcattcag tttacgtacg atttgccgac
     3961 cgtttttctc cattttctct tgcagagtcc cctgcatcag gcccagcaga ttgcggtaca
     4021 gcaggatttt atcttccgca tcaaccgccg cgaccgaatc ttcgcagtcg agaatggtac
     4081 tgatagcagc ttcgacgata acatcgttga tgtgcgccgg atcgtctttg ccaatccgcc
     4141 cgttggcatc aatttgcagt tcaatatgca ggccgttatt tttcagcaaa atgcaggtcg
     4201 gcgcagcggc atcgccacgg taaccgacaa actgtgctgg agtacgtaac gtggtttctt
     4261 taccattttt caactggatg cgtaattgtt tatcgaccac cttaaacgcc accacatcct
     4321 gatagctgcc gttttccagc ggtagtgatt catcgaggaa acgtcgaacc caggcgataa
     4381 cctgctcacc gcgctgcgga tcgtagccac tgaccatcgc cccttcctgc gggatgatgt
     4441 cgctgccgta taacgcatcg tacagtgagc cccagcgagc gttcgccgcg ttcagcgcgt
     4501 agcgggcgtt cattgccgga accaccagct gtggccccgc ctgactggtg atttcgctgt
     4561 caatgcccgt ggtttccacc gtcacgcgct ccggttgcgg caccaggtag cccagttcac
     4621 gaaggaaaga tttataggcc gctttatctt ttaccggccc cggattgctg cgatgccact
     4681 catcaagcgc cgcctgaatg cgatcgcgtt ctgccagcaa ctgacgattt tctggtgcca
     4741 gatcgtgaac gatctcatca acattgtgcc agaatgcagc agcgtccagt tccacgcccg
     4801 gcaaaacttc tccatccaca aaacgtttaa aattggcgtc aatgcgtaaa cggccctggg
     4861 ttatggtttg actcattgtt tctctcctcg ttttcgctta tttcgccaac accgctgctg
     4921 ccactttcgc gacctgcgca tcctgtgctc cggttaaacc agaaacgccc acggcaccaa
     4981 taatttgccc atcaacaaca accggtacgc cgccttccag cgacgtcagt aacggcgcag
     5041 tcacgaacgc ggtacgtccg ttgttcacca tctcttcata gcccttagtt tcacgacgcc
     5101 ccagcgcggc ggtacgcgct ttctcctggg agatgtaagc cgcaatcggc gcgcaatcgt
     5161 ccatgcgact taacgccagc agatgaccgc cgtcatcggc aacagcaatg gaaacagacc
     5221 agttattttt ctgcgcctct tcctgacctg cggcaataat tgcactcgcc atttgctggc
     5281 taagaatgac tttagttttc atttttttat tccttttcaa gggcttgttc tacaatttca
     5341 atccagtgac gcacagaggt acgaccggcg ctcgccagat gcgtctggca accaatgttg
     5401 gcggtgacga tcatttccgg tttgccgctt tccagcgcat tcattttgtt atcccgcagc
     5461 tggcgtgcca gatcgggatg cgttaacgca tatgttcccg ctgaaccgca gcacagatgg
     5521 ctgtcgggaa cgtccgttaa ggtaaatcca agacgaagta acactttttc cacttcgccg
     5581 ttcagctttt gcgcatgttg tagggtacac gggcagtgga aggccagttt tttatcgccg
     5641 cgaattgcca gtttttccag cggttcctcg cgcagaagtt cgactaaatc gaccgccagt
     5701 tcactgacct gacgcgcttt atcggcatat aacgcatcgt ttttcagcat ctgcccatac
     5761 tctttgacga acgcgccgca accgctggcg gtttgcaaaa ttgcctcggc acctgcttca
     5821 atcgcgggcc accaggcatc aatattattg cgcgcccgtg ccaacccttt ctcctgcgca
     5881 ttaagatgat agtccaccgc gccacaacag cctgcttcgt tagctgacat gacgctgatc
     5941 cccagacgat ccagcactcg cgcagttgcc gcgttggtgt tgggcgaaag cgtaggctgg
     6001 gcgcagcctt ccagcattaa aacccgacgc ttatggcgca gcggcggacg cggtttagct
     6061 ttcaccgttt cagcaggcag ttttgctctg acctgttccg gtaaaaacgg tcgcagcacc
     6121 agccctacct gcgtcagcgc acggaagacc gccggacgcg gcactacctg gcgcaatcct
     6181 tcgcgcagca ttcgctccgg cagtgggcgt ttcactttct gctcgacaat atcacgcccg
     6241 atatccagca aattgtgata gcgcacacca gaaggacagg tggtttcaca attgcggcaa
     6301 gtgaggcagc gatcgagatg ctcctgtgtt ttaagcgtga cttcgttgcc ttccagcacc
     6361 tgtttaatca gatagatgcg cccgcgcggc ccgtccagtt catcgcccag aagctgatag
     6421 gttgggcagg ttgcggtaca aaatccgcag tgaacacagg cgcacaggat gctgtcggct
     6481 tccagcgcgc gttctgccgc atctcttcgg ttaatcgggt ttgcatagcc tgctcctcaa
     6541 agttccgcgt acatgcgacc ggggttaaac acgccgcaag ggtcgagctg ctgtttaagc
     6601 tgctggtgat agcggaataa aggagccgat agcggggcaa agccaccatc tccggcacta
     6661 aagcgggtcg catgaccgcc agcgttgcgg gcgatgcgat ggatttgatt gtcatcggct
     6721 gtcgatttca gccagcgtaa cgccccgccc cagtcgatca gttgctcgcc gggtaaatcc
     6781 atcatcggcg catcgctggg taatgaaatg cgccataagg tacctggtaa cgagaagaac
     6841 ggcagttgtt gttcacgcaa ttgctgccag aactgaccca caacctcttc gccacccagc
     6901 agttcacgcg ctgcttttac cgatccttcg ccgccctcaa ggcggatcca caacgcattg
     6961 tcgaagtaac ataagccact aatgggtaat ggctggagtt gccactcggc gatttcactc
     7021 atggcttctt gcaggctgat ttcccgacgc aggctcagag aggcacgcgg tcgcggtaac
     7081 actttcattg agatttcagt gagcacgcca agacaaccgt agcttccggc cattaaccgt
     7141 gagagatcgt atccggcaac gtttttcatc acttcgccac caaaacgcag atgttttcca
     7201 gtgccggtaa tgatgcgcgt gccgaggaca aaatcgcgga ccgaaccgct ccacgggcga
     7261 cgcggccccg ccagcccgca ggcgaccatc ccgccccagg tggcttcttc accataatgc
     7321 ggcggctcgc agggaagcat ttgcccggcg ctctccagcg ccgcttcaat cgccaccagc
     7381 ggcgttccgg cacgagcggt tatcaccagc tcggtcgggt cgtaattaac aatgccgcga
     7441 tgacaacgca catccagcgt ttgcccggtg actgggcgac ctaaaaaggc tttgctattg
     7501 ctgccctgaa tcaccagcgg cgttttatcg ctaattgcct gattcacctg ctccagcagc
     7561 gcctgactgt aatcacactc gcgtagcatc aaaagcgctc cagttcagga aaaggtaaat
     7621 gaccgtgatg cacatgcatg gcaccaaatt cagcacagcg gtgtagcgtg ggaatgtttt
     7681 tcccagggtt cagcaaacca tcggggtcaa acgccgcctt gaccgcatgg aaggtcgtga
     7741 tttcatcgct gttgaactgg gcgcacattt gattgatttt ttctcgcccg atgccatgtt
     7801 ccccactgat gctgccgcca acttcaacgc agagttcgag gatcttcccg cccagctctt
     7861 ccgcgcgggc aaattcacca ggttcgttgg catcgaaaag gattaacggg tgcatgttgc
     7921 catctccggc atgaaagacg ttggcaacac gtaaatcata ttgctgcgat aaacgggcaa
     7981 tgccttccag tacgccaggc agggcgcgac gcgggatggt gccatccatg cagtagtaat
     8041 ccggggagat acgtcctacc gccgggaacg catttttgcg accggcccag aaacgtacgc
     8101 gctctgcttc gtcctgtgcc aaacggacgt cagtcgcgcc cgctttcaac aagatgtagt
     8161 taacccgctc gcagtcttcc tgtacgtcag actccacgcc gtccagttcg cataacaaaa
     8221 tcgcttcggc gtcgacggga taaccggcat gaataaaatc ttccgccgcg cggatcgaca
     8281 ggttatccat catctccagc ccgccgggga taatgccatt ggcgatgatg tcaccaaccg
     8341 caagtccggc tttttctacc gagtcaaagc tggctaacag aacccgcgcc acgggcggct
     8401 tcggcagcag ttttaccgtc acttcggtgg tcacgccgag cataccttcc gatccggtga
     8461 acagcgccag caggtcaaaa ccaggtgaat ccagcgcgtc cgatcccagc gtcagcgcct
     8521 cgccgtccag cgtttgcact tcaattttca gcaggttatg tacggtcaga ccatatttca
     8581 ggcagtggac gccaccggca ttttccgcca cattgccgcc aattgaacag gcgatttgtg
     8641 aggaaggatc cggcgcgtaa tagagattat gcggcgcaac ggcctaggag atcgccaggt
     8701 tacatacgcc cggctgcacg cgcgcgcggc gaccaacggg gttaatgtcg aggctctctt
     8761 taaagcgcgc catcaccaac aacacacctt tttccagcgg cagcgcgcca ccagaaagcc
     8821 cggtgcctgc accacgggtc accaccggta cacgcaggcg atggcagaca gccagaatcg
     8881 ctgtcacctg ttccatttgc ttaggcagaa caaccagtaa tggacgcgtg cgatacgcgc
     8941 tcaacccgtc acactcgtaa ggaatgatct cctcatcggt atgcaggatc tcaagtccag
     9001 ggacatgctc acgcagtgcc atcagtaccg atgtgcggtc gacatcgggt aaagcgccat
     9061 caagacgctc ttcgtacaag atgctcatga gtaggcttcg ctttgttgtg ttgtgtggca
     9121 gctgattttt gcgcgctgct tctgtgaaca gttattaagc gggcttttcg ttttcgtcta
     9181 tctctttagc taccggtcag accatttttt ttccagctct gtgaccttgt cttggttaac
     9241 ttaatgttaa attgatgtaa cataatcact tgcgtgatgt gcgtgttttg cgagttaaga
     9301 acagaaaaat tggtcctacc tgtgcacgag gtccgggaat gaaagatgaa cgtcgcccta
     9361 tttgcgaagt ggttgcagag agtatcgaac ggttaattat cgacggcgta ctgaaggtcg
     9421 gtcagccgct tccctcggaa cgtcgactgt gtgaaaagct cggcttctca cgctccgcac
     9481 tgcgtgaagg gctgaccgtg ctgcgcgggc gcgggattat tgaaacggcg cagggtcgcg
     9541 attctcatgt cgcacggctt aatcgggagc aggacaccag cccgctgatc catctgttca
     9601 gtacgcagcc gcgaacgctg tacgatctgc tcgacgttcg cgcattactg gagggcgaat
     9661 cggcaaggct ggcggcaacg ctgggaacgc aggctgattt tgttgtgata acccgctgtt
     9721 atgaaaaaat gctcgccgcc agtgagaacc acaaagagat ttcgctgatc gaacatgcgc
     9781 agttggatca cgctttccat ctcgccattt gtcaggcttc tcacaatcag gtgctggtgt
     9841 ttacgctgca atcattgacc gatctgatgt ttaattcagt gtttgccagc gtaaataatc
     9901 tctaccatcg accacagcaa aaaaagcaga tcgatcgcca gcatgcgcgg atctacaacg
     9961 cggtgttgca gcggctgccg cacgtcgctc agcgcgcagc gcgcgatcat gtacggaccg
    10021 tgaaaaagaa tctccacgat atcgagctgg aaggccacca tttgattcgc tcggcggtgc
    10081 cgctggagat gaacaaggtg ggaatgtgag cagagtttta ccctatcccc aaggcgaggg
    10141 gaccgcccct gcacttaaca ggatctggtg ttaaatctgt tcatctatac cctctcccga
    10201 atgaccgccc ctgcgctaaa caggatctgg tattaaattt gcttttcata caatcggtaa
    10261 cgcttgtacg gctccgcccc aatgcgttcc agcatgttat tcatgcctgt attggtttcg
    10321 aggatccatg acatctccag cgcatcgatc ttccggcggg caaacggatc gcgtaaggct
    10381 tcaatcaata acagcgcaat caccgggccg atgcggctga actgatactc gtcgcgcacg
    10441 cccatcagcg gtactcgcgc agttcgcaca ccgctgactt tcaaacgcca cagcaatttt
    10501 gcccagccga agggaaagag cgatccgttc agatcggcaa tcgcctcgtt gatgttaggc
    10561 aagccacaat aaacgcgcag ggtgcagaat caatctcagc gatgtagatc atatcgtccg
    10621 gcaccagata tttaagttgg tcgcccatgg tcgcgaattc atgttcggta aacggcacaa
    10681 atccccagtt gtgctgccag ccagagttga aaatctcacg caggatctgc atctcttcgg
    10741 caaaccgctg acgattgatg cagcgaatgg tcaccttttt gcgcacctga tccatcagtt
    10801 tttttagcgc cggagagaaa gtgagatcgg ttcgctgcat ccaccacgcc agtaaatcaa
    10861 tgcctttgtg ataacccagt tgttcaatat gcgcggcata ccacggtttg ccgtgtggca
    10921 tcatcgcaca gggtggtgtg tcaaaacctt caatcagtaa tccgctttcc tgattgatat
    10981 tcaggctgaa aggcccgctg atcttacttg caccttgtga cttcaaccac gcttccgctg
    11041 cgccaaacaa cgcggcaaaa tcctgcggat catcaatggc gtcaatcatg ccgaaatgac
    11101 cggtatcttc accgtaacgc tcacggtgca aggtatctat ttgcgcggta atacgcccaa
    11161 ctatctgccc cgcttttttt gccacccacg cctgccagat gatatggtcc gtccccggat
    11221 ttttcgcaga caaatgctcg ttgcgttcaa tgaataaagg gggtatccag tttggatcgt
    11281 cgggataaag tgatgacgga aaagcgataa atgccttaag gtcattttta ttaattactt
    11341 tttcaatttt aatcatcagc agatcgcatt ccatatctct gtggataaca aataattaat
    11401 ggtgaaaaac aggttgcaac agcaacgagc ggcatcttaa taatcaactc aatgaataca
    11461 aacgacgaat aaatagtatt aattaatgat tatgataagg tcagaggcta cacagaaacg
    11521 cctcaatgcc ttttgtcaat ttaaggcgac cggacaaggc ttcttcttaa tatattcata
    11581 aattaaatat cattcataat aaaaacaaaa aacttaaact cattatttaa atcaatgttc
    11641 attttcacac cgatgaaaat actatctcat ttatctttta tgcctatata atccaccgca
    11701 ttattgtcat tttatttttg atatccaatt ttttcgtggt gtatatgtct aataaaatct
    11761 ttacgcattc cctacctatg cgctatgccg attttccaac gctggttgat gctttggact
    11821 acgccgctct gagtagagcc ggaatgaatt tttatgacag acgttgccaa cttgaagatc
    11881 aactggaata tcagacatta aaaacgcgtg ccgaagctgg tgcgaagcgg ttgttatcgc
    11941 tgaacctgaa aaaaggcgaa cgcgtggcgc tgattgccga aacgagtagc gggtttgtag
    12001 aggctttttt tgcctgtcag tatgctggct tagtcgccgt cccgttggcg attccaatgg
    12061 gcgtcggtca gcgggattcc tggagcgcca aactccaggg tttactggca agttgccagc
    12121 ccgcagccat tatcactggt gatgagtggt tgccactggt caatgccgcg acgcataaca
    12181 accccgaatt acatgtttta agccacgctt ggtttaaggc attaccggaa gccgatgttg
    12241 cgctccagcg tccagttccg aacgatatcg cctacctcca gtacacctcc ggcagcaccc
    12301 gttttccccg tggcgtcatt atcacccatc gcgaagtgat ggctaatcta cgtgctataa
    12361 gccacgatgg cattaaatta cgccctagcg accgctgcgt ctcctggcag cctttctacc
    12421 atgatatggg actggtcggc tttctcctgc cccccgtcgc cacgcaactt tcagtagatt
    12481 atttgcgcac tcaggatttt gccatgcgtc ctttgcaatg gcttaaattg atcagtaaaa
    12541 atcgcggcac cgtttccgtt gcgccgccgt ttggctatga attgtgccaa cgccgcgtga
    12601 atgaaaaaga tctcgctgaa ctggatcttt cctgctggcg cgtcgctggt attggtgctg
    12661 agccgatctc cgcagaacaa ctccatcaat tcgctgaatg tttccgtcag gttaactttg
    12721 acaataaaac tttcatgccg tgctacggac tggcagaaaa tgcgctggct gtcagcttct
    12781 ctgatgaagc ctccggggtt gtggttaacg aagtggatcg cgacatcctc gaatatcagg
    12841 gtaaagccgt cgcgccgggt gcagagacac gcgccgtatc gactttcgtc aactgcggca
    12901 aagcgttgcc ggaacatggt attgaaatcc gcaatgaagc aggtatgccg gtcgcggaac
    12961 gtgtggtagg ccatatttgc atctccggtc ccagtctgat gagcggttac tttggcgacc
    13021 aggtttcgca agacgagatt gccgcgacgg gctggttaga caccggcgac ctcggttatc
    13081 tgctggacgg ttatctgtat gtcaccggac gcattaaaga tctgattatt attcgtggcc
    13141 gtaatatctg gccgcaggat attgaatata tagcggaaca ggaaccggaa attcattctg
    13201 gcgatgcgat tgcttttgtt accgcccagg aaaaaatcat tttgcagatc cagtgtcgga
    13261 tcagcgacga agaacgtcgc gggcagctta tccacgcgct ggcggcacgg atccaaagcg
    13321 aatttggcgt gaccgcggct atcgatctgt tgccgcccca cagtattccc cgaacgtcct
    13381 ccggcaagcc tgcccgtgcg gaagcgaaaa aacgttatca gaaggcttat gctgccagtc
    13441 ttaatgtgca ggaatccctg gcataaatca aactgtcgcg gtgacgggcg ctaccgggtt
    13501 tatcggtaaa tatattatcg ataacctgct cgcccgcggc tttcacgttc gcgcattgac
    13561 gcgtactgcc cgcgctcacg tcaatgataa tctcacctgg gtgcgcggtt cgctggaaga
    13621 tactcattca cttagcgagc tggttgccgg agccagcgta gtggtccatt gcgccgggca
    13681 agtgcgcggg cacaaagaag agattttcac ccgctgtaac gttgacggca gcctgcgcct
    13741 gatgcaagca gcaaaagaga gcggcttttg ccaacgtttt ctgtttatct cttcgctggc
    13801 ggcgcgccat cccgagctct cctggtatgc aaaatccaaa tacgtcgccg agcaacgact
    13861 ggcagcaatg gcggacgaaa ttacgctggg cgtttttcgc ccgacagccg tgtatggtcc
    13921 cggcgataaa gagttaaaac cgctgtttga ctggatgcta cgcggcctgc tgccacgact
    13981 tggtgcgcca gatacacaac tctcttttct tcacgtcacc gattttgcgc aagcagtggg
    14041 tcagtggtta agcgccgaaa ctgtacagac gcaaacctat gaattatgcg atggcgtcgc
    14101 cggtggctat gactggcaac gcgtacgaca gcttgccgcc aaagcccgtt gtggttccgt
    14161 gcgaatggtt gacattcctc tgccggtact cacaggcctt gcggatatca gtaccgcgtt
    14221 gagtcgcctg gcaggtaaag aacctatgct gacccgctcg aaaattcgtg aattaaccca
    14281 cgccgactgg tcggcaagta ataaccgtat ttctgaagat atcaattggt ttcccgggat
    14341 tagcctggaa cacgcattac gcaacgggct attttgattg tcttgtttta aaggtgtgtt
    14401 aacgtttcta tggtaaatcg tgaaatagta atggattaca tcctttcctg tttacaggat
    14461 ttggtcgaaa acggtgtgga aattaagccc gacagcgatc tcgtcaacga tcttggcctg
    14521 gagtcgatca aagtgatgga tctcttaatg atgctggaag acagattcga tatttccatt
    14581 cctattaata ttctgctgga tgtcaaaacc cctgcgcaat taatggaaac tctacttcca
    14641 tggctggaga ataaataatg gggctatacg ataaatatgc gcgccttgct ggcgagcgtc
    14701 tgcaatttag tgacaacggg ttaacaccgt ttggcacctg tattgatgaa gtgtattccg
    14761 ccaccgaagg ccggatcggc aataaaaaag tgattctggc cgggaccaat aattatctcg
    14821 gtttaacctt taatcatgat gccatcagcg aaggtcaggc cgcgttagcg gcccaaggca
    14881 ccggcaccac cggttctcgt atggcaaacg gcagttatgc gccgcacctg gcgttagaga
    14941 aagagattgc cgagtttttc aatcgcccta ccgctatcgt ttttcaaccg gttataccgc
    15001 taatttaggc gtcatcagcg cacttgccga ccataacgcc gtggtgttac tggatgccga
    15061 cagccacgcc agtatttatg acgtctgttc tctcggtggc gcggaaatta ttcgtttccg
    15121 ccataacgat gctaaagatc tggaacggcg catggttcgc ctcggtgaac gggcgaaaga
    15181 ggcgattatc atcgttgagg gcatctacag catgctcggt gacgttgcgc cgctggcgga
    15241 aattgttgat atcaaacgtc gtctcggtgg ttatttgatt gtcgatgaag cgcattcgtt
    15301 tggcgtgtta ggcgcaacgg ggcgcggact ggctgaagct gtcggcgttg aagatgatgt
    15361 ggatattatc gttggtactt tcagtaaaag tctggcctcg attggcggtt ttgctgtcgt
    15421 ttcggaagcg atggaagtgc tgcgctacgg cagccgcccg tatattttta ccgcctctcc
    15481 ttctccgtcc tgcattgcga ccgtgcgctc gtcgttaaga actattgcca cacaaccaga
    15541 gctgcggcaa aagctgatgg ataacgccaa ccatctgtat gacggtctgc aaaaactggg
    15601 ttatgagtta agttcacata ttagcccggt agtgccagta ataatcggtt cgaaagagga
    15661 aggtttacgc atctggcgcg agcttatctc gccgggcgtg tatgtgaatc ttatcctgcc
    15721 gcctgccgcg cctgcaggca tcacgctgct gcgctgtagc gtaaatgctg cccacagtcg
    15781 tgagcaaatc gatgccatca ttcaggcatt tgctactctg aagcagtaat gtgagcccgc
    15841 cgtggtgttt gcgccacggc gggtttaatg ttgtctgtca taccaataca ccaggctcaa
    15901 cgccaccaac acaaacgcta ccggtaaacc gagcgcggta acaggtgcgc ttaacgcaaa
    15961 tagcaatccc agattaacca tgatttgata gctgatccag gtgagtaagc ccacgatgac
    16021 gcctacagcg agacggctac ccatcccagg cgaacgcggg gcactaaagg taaacggcac
    16081 agccagcaaa atcatcgcca gggtcaggat gggttgcccc agtttttccc acagtgcaag
    16141 gtggtattcg ctgctgggtt gcccggtttc ctgcaaataa tggatgtaat gattaagctg
    16201 acggacagaa aaagtattcc ccggcattga taactcttca agattcatac tggtgaaggc
    16261 cgattgccag gcaagattat ctaatgtttc cagcgtttct ttgccattca gccatttttt
    16321 atgattcaca ccatgcagga cccacgtttt gtcgtcttca atggtggcac tgcgtgcata
    16381 aatgtaggat tcaagcgaga gatcgtcgcg ataatgaaat atctccacgc ccaccggctg
    16441 gccctgctcg ttcagcgatt tcaccgtcac aaattcatta ccacgcctgg cccacagcat
    16501 attgccggta atgtcatcgt cctcccccaa cgcggtggcg gtcgatttga tttgcaacgc
    16561 ctgttgctgc aatggcgacg ccacccactc atctatcgcg cctaacgaaa cagtccacaa
    16621 tatcccggca accagcgcca ccagtgcaat acggaagata gaaaaccccg tgctgcgaat
    16681 ggcggtaagc tcactgtttt tcgataactg ccccagtcca acaataccac ccaataacgc
    16741 gataaacggc gaaagttcga ccagtgtgcg cggtaaggtc attagcacca ccagcaccgc
    16801 ctgagtccag cgataaccgc ccgggctgac accatccagt tcgttaatca ggttgaaggt
    16861 ggtaaaaagc ggcagcaata gccctgcagc ggcggcaaaa ccgagaaaga gatgacggat
    16921 taaatagcga ctgaaaacat tcatcgggaa aatttctgca gcaaagagaa gtcacgtgcg
    16981 accagtatca gcaggcccat tagcatgagt ccgggcacta accacaaacc aggaatgagg
    17041 ggaatcgcgc cgttagccac cagtgtacgg cagacgtcgc cgccataaaa aatggcaata
    17101 aacagcaacg tcagtggcaa taacgttgaa aatcgccctt gccgcggttt tacccgactt
    17161 aatgaaatgg ccagcagcgc catcaacaat gcggtcaggc cacggctttg tcgccattgc
    17221 agttcggcat gatcggcagg aaaggcggag cgcgccagct ccgtgaccga tttcgctttg
    17281 cgtttgacgt tcgggctttg atccagcgga ttcagatgta attgcagatt acggtagatc
    17341 tgttcgttgt cgtcacgccc ctgatgatcg agaagatagg cagtgccgtt gtgcaacata
    17401 acggtcggtt tctcgggtga tgggtcaacc acatcaaccg aaccggcgcg gaaaatacgg
    17461 gttcgattgg cagtagaagt ataaatcagc gcgtcagtca ggcgattatt atcctgatca
    17521 accgtctgcg aaaggatcat tcgtccgtta tcgttagtgt taaatttctt tgcccgcaac
    17581 tggcgaacat ccagctccga ctgcgactgc tgctccagtt gataaatctg cgcataagcc
    17641 cacggacgac catacatcga cagaagggtg acaaaaatac ttagcggtat cgccagataa
    17701 agaacggctt tgtacaagcg tcccggactg ccccccgccg cagagatagc ggtaatttcc
    17761 gagtcggtat acatctgccc tagcgtcacg ccaactgaca catacagacc aacaggtaac
    17821 aacatctcca gtgcaatcag tactttgtag aaaacgatat ccagcacaac atcaagagct
    17881 aatgtcccat ttgccgcttc ggtcagataa cgctgtgcgg agtagctggc gaaaataaag
    17941 atcagaaaac cgacgataat cagcaccaga cggcgcgtac cacgcatgat gtagtgttca
    18001 accagcttca tctcactaac gccctcaatc ctttgagttt tttattcatt gatcagtttc
    18061 tgcgccaacg caatgacatg atctacctct tccggcgtca ggctgccgtc ctttgcccac
    18121 tgaatttgcc cggctttatt cagcaccaga atggttgaac tctgctccgg caagcgccag
    18181 gcaacgcgtc cctgtccatt gccgtcaata acgaattgcg cccacggata gcggcgttta
    18241 tttttttcga ttttgccaac aacaaaatag ccagtaccaa agatggcatc atcggtgttc
    18301 acaatggtgg tgggctgaaa acgatcctgc ggaaagtttg ccgcttcaac cgctttaatt
    18361 aacagtgagt tctttttctt cgccgactta cgcccggcga tatactgaat aatgcggact
    18421 tttcccgcta attgtgtgct gttccacttt tggtagtgaa tttcgtcgtc gctattcaga
    18481 agcagctcgc cgccttcctg gatataaaca ggggtaaccg ttttgccagt gacgaaattg
    18541 tgtgcctgca cacccggggc taagagcatg ataatcaacg caataatcaa acgagatgac
    18601 atcattgctc cggctatagc ggaaaacgcg aaatcagcag ctctaagcgc tgacaaatat
    18661 cgctatccag cgccaacggt gcataatacg actggcggtt ttccatatca tccgccacca
    18721 tgtcgtcgtg tttatctccc atcaggccat taatggcggc gaggaaacgg gaaatctgta
    18781 acgcctcttt attttcagga tcaagcgatc ttaaggtgtc gtaataagaa acctgctggc
    18841 tgatgatttt tcgcacatca tgaaattccc tgccggttag tcgcggtttg gcaataatct
    18901 ctttcagggt atgcatttgc ttttgccgat aggcaataaa actgcttacg ctttctaact
    18961 ggctatggcg taatgaatag ttcaccaacg accagaccgg agagcttaaa taaatccgta
    19021 acaggttgcc gtaatacgac acaatatttt tcttaccgtt acgaaagccg tcctgaaaac
    19081 gccccagaaa aacggtcgtt ttgccgaata aaaatccggc ctgatgcttc tttaaataca
    19141 gccgttgcgc aaaacgcagg tgcttgtagc gcgctcgcat gtatttaaat tgtttgcgct
    19201 catcagcagt cagctcatcg cctttggctt gtttgcgaac taaacgcagt aattcctgac
    19261 gatcaacgcc attgagccag aattgcaggc ataacgcgta attatcatga atgatttcgg
    19321 tggtacaact tagtgcaata gtcggcggca aactaacctg tgcatcaacg acatcatctt
    19381 cttcaatcgc aacaaataga cgctcgctcg cctcaagcgg gaaatatttt ctcatcatca
    19441 ggatttctct ccggctgcgt tataaagcaa ggaatggcgc tgaaaaaatg cctttaaggt
    19501 tggaattaac gagagcgcca cgtccagaca ttgcccaccg aagtaagcaa acgctgctcc
    19561 cagcacacca aacagatgac agaacatgaa cagcaatacg atgtagccga ttgaggcgat
    19621 ggtttgcgcc acgagaaaag cgcgctgttt tcccgccatc aatagcaatg actcctgcgg
    19681 gaagcccagc atcgaaatca caattgcgcc cagcatcacc tgaatcaggt catacgcttc
    19741 gagatactta acgccaaaca ccagcgaaat gagcggcttg ccgacgatca acaccgcgag
    19801 cgccaccaga ataccgattc cgcccgccag taaaccggac ttcacaccta acaaccacgg
    19861 tctggtggtg cgcggatcta aacgcatcac ctctgggtaa aaacttttac ccagcaaacc
    19921 cgctggcgtt ccggcggcgt cgaagaatgt catggcgatt ttaaataacc cggcggcagc
    19981 gggtcctaac acgatcccca ccaacactgt gctgcacgag ttacgcgccg accagatgga
    20041 gtgggcaatg ttggttgacc agacaaaact ccacgcgcct ttaatgtgtc gggcagactc
    20101 aaacagattc aatttgaagg cgttatggat atttctacgg cgtaattcgc gcgcggcaaa
    20161 ccaccagtac atggtgccgc caaccagatt cgacacgtac caggcaataa caaaacccac
    20221 aaaaccaaag tcaaaatacc aggctgcgac gctccccgct gcgcgcagaa aaggtttcgt
    20281 cgcctgctgt acagcaatca aatcaaagcg atctaccgca cgcagaatgc cggtcggcgt
    20341 ggaggaagcc attgaaggaa tgagcgtgca atacagcgcc gccagccaaa agctttggtc
    20401 atccagacct aatgaatggg aaaggaatgg cagtaaggca atgccgccga caatcgccac
    20461 cgcgccgctg acgatatcca gcgagaagga aaatgagacg acattgcgaa attgctgcgg
    20521 attattgttg gtcaaggctg gcgttccgta ctgaaccacc agctgccatg tctgaaactt
    20581 aataaaatcg ctgatcgact tggcgtacga ttgcacaatc accagtacac caaacatggc
    20641 gggcgtcatc cctttaccgg cacacgagag cgccaacagc cccagcaagg cgctctcgac
    20701 attactggag cctaaccagg cgctattgcg aataatggtg cgaaacgcgc catctgcaaa
    20761 ccaatgtttg atgttaaaac ccgccaattc agcctgacct tatcgttgat agtaaaaaat
    20821 atcccgccag ccttaagtta aacttcggcg gtcagaaacg atggcaacca gagaaaccgc
    20881 cttctgtacc tgttccagca cttcgctgta gggcgctctg gaatcaatct caagaatttt
    20941 tgtgccgtta tagccaatcg tcgacatgac gccgattttg tcctgcagct cggcatactc
    21001 atggtcaggc ttgcgggaga tggcagtatc aatatcaatc cccaaacgaa tgattaattc
    21061 aggacggtat tgcgccattt tttggtataa actccgttcg cgctgcgcca gaaacatgct
    21121 gattttcccg gtcgcacgtt cgacgccgat ccccggtccg tcataataaa agcccgaaat
    21181 ttcagcctgc gggaagcgat cgctgaccac cagaacgcca ctttgcgcca gtcgctgaac
    21241 cttgcgtaga ttcgccattc ttcgcagcga gaagcagtac ataatcaccg ccgcccatag
    21301 cgccggagat ttggttttca tgctttgggt tttcgaggat ttggccgcca atcgccgttc
    21361 cagccagacg ccaaccaacg gcaatcgttt gattttgtcg ccgtcttcgc cggagagcag
    21421 ccccagatag cggcgctcgg tttgccagtg ttgttgcagc gatttcacca ggtcggtggt
    21481 cagcgtggat ttaccggtgc catcacaccc gaccaccgca atcagccccg gaatgtagtt
    21541 gggctgcggc gcggttgtac tattaacagt ttgtgtttgt agtgcatcca ttcgttaaat
    21601 catcccctat ccattttggt gatcaggaaa gcgcggcgtg aattgcgcgt agtgacgctt
    21661 gcataatttc atcggctggt tgccgaccat ccagttcaag gatcttggcg ccattgaatg
    21721 tcagttgcgg cgtaacggcg attttttcct gtaacgctgc cagttggtgg tcaggtttac
    21781 gcgcaaacgc ggtttgttca tcaatgccaa gacgaatcaa caatacgggt aaataagacg
    21841 ccatccattg gtacagcttc aactcgcgct gccttaacat ttttatccaa ccgttaccgc
    21901 ccgtggtttt tgccaattgc gggccatcaa agcgaaaacc cggcacttca acctgcgggt
    21961 agcggtcggt gatgagcaga tagccttgct ggcttttaca caacattttg cgaaacttgt
    22021 acgcccgcca gcaggaaagc agatagataa ccagcgcagt aatattgcca ggcggagtta
    22081 agggcttttc gtgtacatgt gccgctttac ttcgcagata acacccacaa ggtgcgccaa
    22141 taacagggag ctgtgaaatc cattcgccaa ttcgcccgga cgattgcccg agataaatgt
    22201 gttctgttgg cattcttgct gccagttcat ttaccaggct tgccgtgagg gtcgatttac
    22261 ctgaaccatc acatcctaca atggcaataa cacgcactgg cgtggaatta attattgaca
    22321 tattacgttg attcacgaaa aacccggcag taaataatgt attgaatatt acgtggtccg
    22381 tgctcagaat atccgttcaa ccttattgtg acaatgacta attgtgacat tgacctgaat
    22441 tacgctttca taaaaacata ttaaccaaat aaatattttt aatggatatt taaattaaag
    22501 gatatattca tgcagtcaat aacacctcca ttaattgccg ttattggtag cgatgcttca
    22561 ggcaagtcaa cggtgtgtga acatcttatt accgttgtcg aaaaatatgg tgctgccgaa
    22621 agagttcatt taggaaaaca ggccggaaat gtcggtcgtg cagtgacaaa attacctttg
    22681 atgggaaaat ccttacataa aacaattgaa cgaaatcagg tgaaaacagc aaaaaaattg
    22741 cctggaccag ttccggcgct ggtaattaca gcgtttgtcg cccgtcgctt actgcgcttt
    22801 cgtcatatgc ttgcctgtcg tcgtcgcggg ttaattgttc taaccgaccg ttatcctcag
    22861 gaccaaattc ctggcgctta cgatggtacg gtgttcccac ctaacgttga aggtggtcgt
    22921 tttgtctcat ggctggcaag ccaggaacgt aaagcgtttc actggatggc gagccataag
    22981 cctgatctgg tcatcaaact caatgttgac cttgaagttg cctgtgcacg taaacccgac
    23041 cataaacggg aatcgctggc gaggaagatt gccataacgc cacagttaac ctttggtggt
    23101 gcacaactgg ttgatatcga tgccaatcag ccactggaac aggtgttggt tgatgcagaa
    23161 aaagcgatta cggattttat gaccgcgcgt ggttatcact agtcaaaaat ggaaatgccc
    23221 gatcgccagg accgggcatt ttcaggaagg ttaaatcaac tgcaatgcta tccagtacag
    23281 cccaccagaa agaaaaattg ccgccggtaa agtaaacacc cacgccatca ggatgctggt
    23341 taccgtttta cgctgtaacc caccgccgtc caccaccatc gtccctgcta ccgcagacga
    23401 gaggacgtgt gttgtggaga caggcatccc aatataactg gcaaggccga tagacactgc
    23461 cgccgtcatt tgtgccgcca tgccttgcgc atacgtcatg ccgcgcttac caatcttctc
    23521 accgatggtc atcgctacac gacgccagcc aatcatggtg ccaatgccga gcgccagtgc
    23581 taccgccatg atgatccaca ccggagcgta ctcaatggtg cttaacatat cgctgcgcag
    23641 ttttttcagc aggttctggt cttctttact gacgcctggc agtttcgcca gcttcgcgga
    23701 ggtatcggag atgcacagca taatgcggcg cagctggctg cgctgactca cgcttaacgg
    23761 ctcgtaactt tccatattgc ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt
    23821 atttgccgga tgacagtgaa actctgttac ttgcgtgcca tcagtcgatg ctgcaggcaa
    23881 tggaggttcc atcgcaatca acttctgcgg cagttcagga tgctgttgca ggtagtgttc
    23941 gaagttggta acggcatcgc gggtacgggt aatttcatag ccggacgcat tcatattgac
    24001 gacgaagcca gcaggggcaa tccccaccag taccagcatt accaggccga tccctttctg
    24061 cccgtcgttc gcgccgtgcg aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg
    24121 cgtccagaat ggcggtttac gtttgccctt tttctttttg cgatcttccg gaatgcggtg
    24181 aatacggtca cgctttttcg tcccactcca gaaacggcgc agcaggaaaa tcagaccacc
    24241 cgcaatgacc aggccgacga taggggaaac aatcagcgag gagaaaattt tggttacttc
    24301 acgcaggttt aacgcatcca tcaccgatga gccggttaac agcgcgttgg ttaaaccgat
    24361 gccgataatc gcaccaatca aggtgtgcga actggaggcc ggtaaaccaa agaaccacgt
    24421 tcccaggttc cagataatcg ccgccagcag catggaaaag accatcgcca aaccgtgagt
    24481 tgaccccata ttcagcaaca aatcgattgg taacatatgg acaatggcat aggcaacgct
    24541 aagtccgccc aataacacgc caaaaaagtt aaaaaatgcc gccatcacca cagcaagttg
    24601 tggctgcatg gcacgagtat aaataacggt tgccaccgca ttcgccgtgt catgaaaacc
    24661 attgattgct tcgtagaaca acacaaatgc cagagcaagt aataacaaaa gccccgtata
    24721 tatatcaagg ccaacaaata aatttagcat atattagatt acgccatttt gaatttacga
    24781 acggacgcat tatcagtgac tttaacggca tgggcaaagt gaaatatcat tttttaacga
    24841 taaaaagagt gttatttctt gtgtatcaga caataagcat tcattcaaaa cctttagaaa
    24901 taatcaaaac acaggtattc cactggttaa tatattcaca tatgaaatga ataaatattg
    24961 gaatatataa atattgaata ttttgattaa tccccgtact aattattctt cataatcagt
    25021 acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa tatcactctc
    25081 ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt tgccgccaac
    25141 ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc acagttgctg
    25201 atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct cttcatgatg
    25261 gctgaccagg tcgatattgc taccacagcg accggcgatc ggtttcactg cgtaacctgt
    25321 tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat ggtgcgggaa
    25381 cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc acagcggctc
    25441 aaagaccagc acttccgggc gcagcaatac gtcgataaga cgcacttcgt tttgcggatg
    25501 accggtacgg attggcaccg cagcaaactc acggtcgcta acttcacgaa tctgatcaaa
    25561 cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt ccccatcaat
    25621 cagttgcccg gcagcatccc agcccagttc atccagcccg cgcaagatac gcgtttcaaa
    25681 gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct cgatatcttt
    25741 gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac cagccagttc
    25801 gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct ccgcccaacg
    25861 ttcgaggatc aagcccgctt cggtatgaca ggaggcggag tcggcgttgt attcgtaaac
    25921 cttcaggcca cgctcatcca tgcagaaatc catacgacca gtgatcatat ggtgacggcg
    25981 acgctgccag gagagacgca aacgtggcca gaggattttc ggaatgtcga acagcgccag
    26041 caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcagat gcagctcgtt
    26101 ggtggcttta attagctcct gctcggcact ctcggtaatg gtgtagtaat gatacggatc
    26161 ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgtagcggat ctttttcatc
    26221 cagccattta ccgtcaaact ggcctttgtt ttccagacgc gctccactga ttttcagcag
    26281 ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct ggatcatcca
    26341 gcccagaatg gtggtgtcat caaaagtgtc tttaagggta tagcagccgt tttcgaccac
    26401 catctccagc tcgcgcgtcc actgttgccc ttgcggcaac ggggaatgaa tcacgttctg
    26461 ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat ggccagtgtc
    26521 tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg cacgcggcga
    26581 gccgttagga aatgcctgca atggcaggat gttgtcatta accacttctc gcaggaagcg
    26641 cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt aattcagaaa
    26701 gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg cccatatatt cgtcgtcgat
    26761 atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac tgtaatctga
    26821 agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg gggcatcctg
    26881 gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc agttggcgta
    26941 attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca gactaatcat
    27001 acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg
    27061 acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac agacaatact
    27121 tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt cgccaatatc
    27181 aatcgcccgt tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa acatccattg
    27241 caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct tgaggagcta
    27301 ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat tggcgatggc
    27361 gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc ggcgctgcgc
    27421 gatcatacgc ataatccgcc gatccgcgtg tttgaatctg gttcgatcct gctttatctg
    27481 gcggagaaat ttggctactt cctgccgcag gatctggcaa aacggactga aacgttgaac
    27541 tggctgttct ggttacaggg tgccgcgccg ttcctcggcg gtggttttgg tcacttttac
    27601 cattacgcgc cggtgaaaat tgagtacgcc atcaaccgct ttaccatgga agccaagcgc
    27661 ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg cgatgagtac
    27721 accattgcgg atatggcgat ttggccgtgg tttggcaacg tggtgctcgg cggcgtgtat
    27781 gatgccgcag agtttctgga tgcgggaagt tataaacatg tgcaacgctg ggcgaaagaa
    27841 gtgggcgaac gtccggcagt gaaacgtggg cgtattgtta accgcaccaa cgggccgctg
    27901 aacgagcagt tgcatgagcg ccatgacgcc agtgattttg agacaaatac agaagataag
    27961 cgtcaggggt aagggttggt gttcgtcgca gcaagccatc caggccggat aaggcgttca
    28021 cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct tatcaggcct
    28081 acaaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat
    28141 gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg taccagcacc
    28201 cactggccca gcagatcggc agggttacct tcgcaaatca gggcgatatt cacgtcgcgc
    28261 ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac agccagcacc
    28321 tggcctggaa cgccaataca cattgttaac tccggttatt tcgattgccg gacaaacgcg
    28381 aaaaagaacc tttagcgggt gttccctctc accctaaccc tctccccgga ggggcgaggg
    28441 gaccgtccgt gcgcttttta ggcgttgtcc tcagtcttat tcaacttcaa tacttttgac
    28501 gatcagcgaa tcgccggtat cgacacgcaa ccgctcgccg tgacagagcg gacactgcgc
    28561 atcgtgctga tgaatctcca ccacctggct gcaatcccag caccaggcct gggcgggttt
    28621 atagacgata tgtaaatcgc acccttgcgc caccgttccg tggcagacaa tttcaaaact
    28681 aaaacggacg gcgctctcct caacgcagga gagcgcgcca atttccagcc acacggcggt
    28741 gacgcgctta acatcgtgct gctccgcctg ccgttggata atttcaacgg cgctctggca
    28801 aagcgacaac tcatgcattt tcgcccctcc tgcggccaaa cagcagggcg cgacgccctg
    28861 cgtgtggtac atccggattg gtgactggca acgaaaggat cattcgtgca cagtcatccg
    28921 tcaggcgttg gccttcttca atcgacatgc tgtgcgaaag cggcgacatc agcgaacagg
    28981 agaggtattg cgaaacaccg tccagttcgc caacggtaaa ggtcatggtg ccatacggca
    29041 gttgcagacc aattttttca ctcactttgc gcagcggcca gagttgatcc gggccgggga
    29101 agataactgc actcagcatc cacggggtga tcacacaccc cgtccactga ccttcgaaca
    29161 gcgtaaaatc agaaacatac accggcattg aaggatgcag aaaagagaga tcgtgcatcg
    29221 aacgccgggc aatttcttca aacgctgcct gtacttgcgc cttcggggag gtctggaaac
    29281 ctgctatctc ttcagtcatg aatcgcctcc cgtgggatgg cttccacgcc tgattcgcgc
    29341 agcgcagcca gaacctgctc aagcgcaggt tcaatcattg cttcaaccgt cggcgttaag
    29401 ccgatgtgtg gctccagcga ttccgggatc acgccgacca gggtcagctt tttcggaaac
    29461 tcaccggtga agcgcagggc cgacaatacg tcggccaggc caagctgatg cggagagatt
    29521 ttgttggtaa acaacgccgg aacttcttca tcccgcagga tcatcatcgt tcccggagtg
    29581 ttctttttcg acacaatggc atccgcaata atcagatgat cgcgatttgc catgtcgcca
    29641 agcagctcca ttcccgccgt gccgccatcg aggatctcaa cataatccgg cagaatgtat
    29701 cgttgctcta acgcttcgac aatccgcacg ccgatggctt catcggtcag caaaatattg
    29761 ccgaccccta agactaaaat acgcattaca gaaccttcac tgaaaccact tcgttgccgt
    29821 cggcatccac tacgtgtacc gcacaggcca tgcacgggtc gaaggagtga atggtacgca
    29881 ccacttccag cggtttattc ggatcggcaa ccggtgtacc caccagcgac tgctcgtaag
    29941 gaccgacgtc atcattgaag ttacgcggac cagagttcca ggttgatgga acaaccgcct
    30001 ggtagttgct gatgataccg tctttaataa ccatccagtg agagagcata ccgcgcggcg
    30061 cttcgaggaa gccaacacct ttgaactcac ccgttgccgg aatgttcggc ttcacaaagg
    30121 tggtgtgatc gcctttgccg atattggtga tcagtgcact gtattggttt tgcaggatat
    30181 cctgcaattc acagcagtga acggtacgac caataatacg gcccagcgtg gagtgcagct
    30241 gtgccacttc cagcgtgttg ccagtcagtt tctgataaat cgcaacgatt tcattcagtt
    30301 tgttctgggt agattcgcga cctgccgcca gtttcaccag catattagcc agcggaccca
    30361 cttctaccgt tttgccgtag aaagtcggtg atttcaccca ggaatatttc ccgtcgtcag
    30421 accaaccatc ataagccgga atggtggtac cttcccacgg cgcctgtggc gcttcgtctt
    30481 tataccagga gtgcttcgcg ctttcctgaa tgcctttgat caggtattca tcggaatgag
    30541 aagtgatcgg acgatacgag gacagatccg cattctcaat gtagcctccc gggaacagga
    30601 agctgccgtt tttgctgtcg gtcgggaatt ccggcacgct caggtagttc accgcacctt
    30661 taccgcgcgt cagccattcc gggtagaacg cggcgataac cgcggtatca accttataaa
    30721 cctgctcaac aaagtcgctc agtttgtcga tgaaagactt gatgtacatc aggcgctcaa
    30781 ggttcaacac gcccaacccg tcgaggttga ttgggttcgc gacaccaccc acagccaggt
    30841 tctgaatgtg cggcgtttta ccgcccagca gcgccacgac gcggttagcg tcacgctggc
    30901 actccagcgc ctgcaggtag tgcgctaccg caatcaggtt cacttccggc ggcagtttca
    30961 tcgccgggtg accccagtag ccattggcga aaatacccaa ctgaccgctg gcaaccagat
    31021 ctttgatctt gttctgaact ttagtgaact cttccggact gttcagatgc caggtcgaaa
    31081 cgcctttcag catttcggag gctttggttg ggtcagcttg cagtgcagaa gtgatgtcca
    31141 cccagtccag cgccgaaagc tgatagaaat gaacgatatg gtcatgcgtg gtgtgcgcag
    31201 ccagaatgat gttacggatg tattgcgcgt taaccggaac gtcgatattc agcgcacttt
    31261 ctgccgcacg aacggaagac agcgcgtgag tggtagtaca tacgccacag atacgttgca
    31321 caatcatcca tgcatcgcgc ggatcgcggt ttttcacgat ctcttccatg ccgcgccaca
    31381 tggtaccgga agcccatgct ttcgaaacga cgccattttc gatttcgcaa tcgatgcgta
    31441 aatgcccctc aatacgggtt accggatcaa tagtaattct ctggctcatg ctttgctcgc
    31501 ctcatgacga ttatgatcgt tttgttttaa aggaggaagt atcggcagta gacgaatgag
    31561 tacgatgtaa gcgcaaatct caatagccac aaaaccaata gaaatcaaca gttcttccca
    31621 ggtcgggaag taggcgtaac cgccgcccgg gttgaatgcc accaacgaat aggtcagacg
    31681 ccaggttgca caacctaaca gtgcgctcag tgctgacagg aacagcatac gggaatcatt
    31741 acgcagcttc gccacacgca gaacgaccag cgggaagagc atcagcagga cttcaatcca
    31801 gaacatcacg gagtagaagt caccggcaaa cgctaacgac agcttgtcgc gatagataag
    31861 ctcgccaaag cgcagcccga tgaaaatcgc cagcaacaca ctgatggtgt tggtcagctt
    31921 gacgaacagg ctcttttcat ccggaccgtt gccacgcaga cccgcctgca ccagtgaacc
    31981 ttcaaagatg acaatcgaga agcccatgat gaacgccgtc agcagcgaga acagcggcaa
    32041 catttcatag ctctgccaga gcggatgcac cttgtagccc gccgagatca tcagcgaccc
    32101 cattgaagac tggtgcatgg tcggcagcag cgcaccgagc gcgatgatga agaacatcac
    32161 cttgttcaga cgttgtagcg acaccttcca gcccagacgt tcaaacagtg ccggagcaaa
    32221 ctccagagcc atcacgccga tgtagatggt catacagacc gccgtctcga acagtaccga
    32281 gttcacgttg aagtgacccg gaatgtagaa gtacggcagg ttccagtagc gacccacgtc
    32341 gatagtgatc gacaagccac ccagtgagta accaaacaga ctcgccaaca gcgccggacg
    32401 caccagcgga tggtattgcc cacggttaaa gacgtatacc gcccacgcca gcgcccagcc
    32461 gccacaggca aagccggtgc caatcagcag gtcaaacgcg atccacacgc cccacgggaa
    32521 gccgccgttc aggtcagaga cagagcccag accgaacacc agacgcttca caatcaggag
    32581 catacagatg acgattaacg gtccaaaaat catgaccggt ttactgatga ttttgccgcc
    32641 cagcggttgt ggatcatgac tcatgatcgt ctcctccgtc gtgatggtcg tttttggtgt
    32701 tgcgacgaac cagcacggtt aagcccgcca gcacagccag tggtagcatc atgcctttat
    32761 acagggtgtg ttgaatattt tcggaacgcg caccggtaga aagatcgtcc agtttcggca
    32821 ggtcgagatt ttcataaggc acacccgtca gtaccagaac ctgagtaccg ccgccctctt
    32881 tctcgccgta cagatgcgga taatatttcg gcaccgtatg caggtaagtg tcgccagatt
    32941 tcagcgtctg acgcggatag tggtattcgc tgccaggctt cagcgccaga cgttttttcg
    33001 cctccgccat cagctcttca cgcgtaccga aaatcaccgc gcccgccggg cacacttcta
    33061 cgcagccagg cagaccgcct ttatcgagac gttccacacc tttctggttg cacagctcgc
    33121 acttatgcag cgcaccaaac gggttgttgt agtcgtactt cggcacgttg tacggacagg
    33181 cgaccatgca gtaacggcaa ccggtgcaca cgtctttgtc gtaatggaca atgccggttt
    33241 tcggatcttt tttcagtgca gagaccgggc acacagagac acagttcgga tcgacgcagt
    33301 gcatacactg tttcttaatg tacgcgtagc cgttctcctc ctggtctttg ttgacccctg
    33361 tgccgctggt ccacacctga atgatgttat tggtatacgg cgacagtttg tcgttgttcg
    33421 accaggtctg ttccccttgc gggttacgtt cagggaagtt gatatcctga cacttggtga
    33481 cgcaagcctg gcagcctacg cacaaggtcg agtcgtacaa catccccagc gatcccggaa
    33541 ttggcgggcg gttttcagca gccgcatgac tgacagacgg cagcgcgccc gtcagcaatg
    33601 ccccgcagga ggctgcttta ataaaattac gtctgttcac ggttattctc cccgtgagtc
    33661 agcgttatct ttcttttgct gacgacccag ttcacgcacc gccatcacgc tgacaccggc
    33721 aaccagccca accacaccgc cgagcaaacc aatagcgcct gcagagacgt tgccgccctc
    33781 tttagcgtta acatccggtt tctgtgaacg cggagtctga ttttcgacgt tggcaagctg
    33841 atggatgcct ttatggaagc cgataccttt ttcgttacag ccatagcacg ggtgaccaat
    33901 cgccaccggc cacacaccgc caacatcgca gaattgcagc gttgagcagt tgccgtaagt
    33961 ttctggccct ttacagccga ggtggtacaa gcaccagcct tcgcggtggc cttcatcacc
    34021 gaactctttg gcaaaacgac cagcatcgaa gtgcgggcga cgttcgcagt gttcgtgaat
    34081 cagacggcca taggcgaagg tcggacggtt tttgtcatcc agtttcggcg gtttgccgta
    34141 agtgatgatg tgcgcaacgg tcgcgaggaa gttgtgcggg ttcggcgggc agcccggaat
    34201 attgataacg gttttgcctg gcagaacttc ttgcaagctg actgcgccag ttgggttaac
    34261 tccagctgcg gcaacaccgc cccacgcgga gcaggaacca atagcgataa tggctgctga
    34321 gccttccgcc gctttgcgga tgtgatccac aatcggctca ccggcaacca tgcaataaat
    34381 accgttatct tttaatggga tggaaccatc caccactaac acatactgcc ctttgtactt
    34441 ctcgagagcg ttatgtttgt tctcttcgac ctgatgaccg aaggcggcgg aaagcacttc
    34501 gtgatattcc agagagatag tctccagcac gaggttttct accgttggat gcgttgcacg
    34561 aagcagagat tccgtacaac cggtgcactc ctgcgcgcca atccagataa ctggcggacg
    34621 ctgcgggtta gtaaccgatt cggccatctc tgcagcggct ttgctactta accccatggt
    34681 ggcggctaat gctgcacaaa gcttcatgaa atcacgacgg ttaatgccgt gagaatggat
    34741 gagggtgtta tctccagtca tttatagtta ttccgttgcg agaacctggc atatattttg
    34801 cctcaatcgc aaaatcaata atgcgatcga tgcgccattt accacacatt tattatggtt
    34861 atcgatatca tgatactgcg gcggggcgct aaaacgaagg gaagaagcat tagtgtaatt
    34921 aattaaagca agataatacg tatgtttgat caattttcga ggcgataacc ggccataaaa
    34981 aagcgcaacg tagaacagga attattctca cgaaagaaat ccattaccat ctctttatcc
    35041 agtccatagc ggcgcgtcag aatacccgct tcccaggcgc taagctggcg atctccggtc
    35101 ttttcgtaca tcctgtgtga gtagcctaat acaaaaccgc gtttataatc ggcacagaat
    35161 tttgtcacat taaccgcact gtcggcatgc gttgctttta atcccgccat taagccttta
    35221 ccaaaatggt tattcatcgt tcacctcatt cattatatat cgccatatat aacgatattt
    35281 taggcaaaga acgacttttg cgcacgctat tttttcatac ttgtttaaga cgtaatcccg
    35341 taagagataa acctatcagg agagatattt atcgaaccat ttcagtgtcc tttgccaggc
    35401 aagatcggcg gcagatttgt cataacgggg cgtggaatca ttatggaatc cgtgattaac
    35461 ccccggatag atataagcct cataaacctt attattgtct ttcaacgccg cctcgtaagg
    35521 aggccagccc tcgttgattc gggtgtccag ttccgcgaag tggagtagta aaggcgcttc
    35581 aatcttcgcc acatcggcag tgggtgcctg acgaccataa aacggcaccg cgcaggccag
    35641 ttccggatac gcgacagccg ccgcgttcga tacgccaccg ccatagcaaa atccggtaat
    35701 acccacttta cctgtcgctt gcggatagcg ttgcataaac tcaattgcgg caaagaaatc
    35761 attcatcagt ttggttggat caacctgctg ttgcagctca cgacctttat catcatttcc
    35821 cggataacct ccaacggaac ttaatccgtc aggtgccagg gcgatatacc ccgccttcgc
    35881 cactcgccgt gccacatctt cgatatacgg attcagtcca cgattctcat gcaccacccc
    35941 cacggctggc gttttgccgc tcatttttgc gggtttcacc agataacccc gtacctcgcc
    36001 gtgaccattt ggcgaaggat acgtgatgta ctcagcaaca atctccgggt cggtaaactc
    36061 tacctgagtc gccagcgcat aatttggctt gagcaaatca aacaacgcta atgccgtcat
    36121 cccgcccacc gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg
    36181 aacgtaatag tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg
    36241 catcgttgca ttcctcaact ggtgtttttt taagcaaagc ataagcgcgt gtttttgccc
    36301 agtttttcgt cactctgtga gccagactac gggatacgcg ctggcgaatc gctaaactag
    36361 aaacattgtt tcgaaattga acggtggaaa ggagaggtca tggtctggtt agcgaatccc
    36421 gaacgttacg ggcagatgca gtaccgctat tgcggaaaaa gtggtttacg cctgcccgca
    36481 ttatcgctcg gtttatggca caatttcggt cacgttaacg cgctggaatc acagcgtgca
    36541 atcctgcgta aagcgtttga tttaggcatt acgcactttg atttagccaa caattacggg
    36601 ccgcctccag gaagcgcaga agagaacttt ggtcgcctgc tgcgcgagga ttttgctgct
    36661 tatcgcgatg aactgattat ctctaccaag gctggctacg atatgtggcc cggcccgtac
    36721 ggctctggcg gttcacgtaa atacctgctc gccagcctcg accaaagcct gaagcgtatg
    36781 gggcttgagt atgtcgatat cttttactct catcgcgtcg atgaaaatac gccgatggaa
    36841 gaaaccgcct ctgcgctggc tcatgcggta caaagcggta aggcgctgta tgtcgggatc
    36901 tcctcttact cgccagagcg gacgcaaaaa atggtcgagt tgctgcacga gtggaaaatt
    36961 ccgcttttaa ttcatcaacc ttcgtacaat ttactgaacc gctgggtgga taaaagcggc
    37021 ctgctggata ccctgcaaaa taacggcgtg ggctgtattg cctttactcc tctggctcag
    37081 ggattgctga ccggaaaata tctcaacggc attccgcaag attcacggat gcatcgtgaa
    37141 gggaataaag ttcgtggtct gacgccgaaa atgcttaccg aagccaacct caacagcctg
    37201 cgcttattga atgaaatggc acagcagcgt ggacaatcaa tggcgcaaat ggcgttaagc
    37261 tggttgctga aagatgatcg cgtgacgtcg gtattgattg gtgccagccg cgcggagcaa
    37321 ctagaggaga acgtgcaggc gctgaataat ctgacattta gcaccgagga gctggcgcag
    37381 attgatcagc atatcgccga tggcgagctg aatctgtggc aggcgtcgtc cgataaatga
    37441 cctgttaata acgggcgcgc gaaccgcgcc cgaatcagat cagtgattat gacgagtcag
    37501 tcggtcaaga tagcccatca caaatgcaga aagcacaaac gtcagatgga taatgacgta
    37561 ccacatcagt ttgttatccg ggacattttt cgcatccata aagacgcgca gtaagtgaat
    37621 ggaagaaatt gccacaatcg acgctgctac tttgtttttc agcgacgttg cgtccatttt
    37681 ccccagccac ttcagcttct ctttgttctc ggagatatcc agctgtgaga cgaaattctc
    37741 ataaccggaa aacatcacca tcaccagcaa accgccaacc agcgtcatat ccaccagcga
    37801 cagcaacacg aggatcaaat ctgattccgc catcgagaag atattcggca gtacgtgaat
    37861 aatctcctgg aagaacttca gcgccagggc aactaacgcc agcgaaaggc caaagtacac
    37921 gggggcaagc agccagcgag aagcatacat tgcattttca agaaaacgtt ccatacattc
    37981 ctgtcgtgta gttaaaacgg gcaacagtat atcgcaattg agtaaacagc acgcaacagc
    38041 aaatcaacat atacgttaaa cctttgtcgc ataattctga ggtttaggga tcttcctggt
    38101 tcccttatca acgcatcgag agcgtctacc cttaaaggac gttgaacctg aagggagaaa
    38161 aacgatgtct catttaaaag acccgaccac gcagtattac actggtgaat atcccaaaca
    38221 gaaacaaccg acgccaggca tccaggcgaa gatgacaccg ataccggatt gcggcgagaa
    38281 aacctatgtt ggtagcggtc gcctgaaaga tcgtaaagca ctggtgacag ggggcgattc
    38341 cggaataggt cgcgctgccg ccatcgctta cgcgcgtgaa ggggctgacg tggcgatcag
    38401 ttatcttccc gtggaagaag aagacgctca ggatgtgaaa aagatcattg aagaatgcgg
    38461 acgcaaagcc gttctgctgc caggcgattt aagcgatgag aaattcgccc gttcgctggt
    38521 tcacgaagcg cacaaggcgt taggcgggct ggatattatg gcgctggtcg ccgggaaaca
    38581 ggttgccatt ccggatattg cagacctcac cagcgaacag tttcaaaaga cctttgccat
    38641 taacgttttc gcgctgttct ggctaaccca ggaagcgatc cccctgctac cgaaaggtgc
    38701 aagtgtcatc accacttcgt caatccaggc ataccagcca agtccgcatt tactggacta
    38761 tgcggctacg aaggcggcga ttctgaacta cagccgtggc ctggcaaaac aggtcgcgga
    38821 gaaaggtatt cgggtgaata ttgtcgcgcc aggccctatc tggacggcac tgcaaatttc
    38881 cggcggacaa acgcaggata agatcccgca gtttggtcag aaaacgccga tgaagcgtgc
    38941 ggggcaaccg gcggaactgg cccctgtata tgtttatctg gcaagtcagg agtcgagcta
    39001 cgtcaccgca gaagtgcacg gcgtgtgcgg cggcgagcat ttaggttaaa aaaatgcccg
    39061 gttgtgaaaa gcaaccgggc atcattgtga attcactctt acctgatgtg gcgaaaacgc
    39121 cttatccgcc tacaaacgca tgaagattca acatgttgca atagattttg taggcctgat
    39181 aagcgtagcg catcaggcat tgagcaccta atgccggatg cgtcgtgaac gccttatccg
    39241 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    39301 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    39361 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    39421 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    39481 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    39541 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    39601 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    39661 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    39721 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    39781 cgaaccggat gcgcagcggc gctggcttcc agatctaggt cacggctttg cagcaacaat
    39841 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    39901 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    39961 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    40021 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    40081 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    40141 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    40201 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    40261 gcggcgatat cgttggcctg gtttaaggaa cgtgcttcag ccagcagttg ctgctcgcgc
    40321 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaggtg
    40381 actacagagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtactga
    40441 tacatacccc agacggaaag gtccgtctgc attaaattat tacccactgt gtatctccag
    40501 gacgcaagtc acaaaatctg cgcataataa tatcaaaacg acgtcgaatt gatagtcgtt
    40561 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    40621 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    40681 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgacat
    40741 ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    40801 tggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtatttgaga gtatggaagc
    40861 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    40921 gttaacccat ttttccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgc
    40981 gctatttccc tgcggggcgg cagcggttgc taattcgatt cttgcttttg tcgaacaggg
    41041 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    41101 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    41161 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccgggtt ccatcactat
    41221 ggaagtccac gacgttccgg cgattgttgc cgccgtgcgc agtgtggtgc cggatgccat
    41281 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat
    41341 cgatgtttct attcaggccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    41401 cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaatgcct atctgatggg
    41461 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    41521 gcgtttgcgt caacatcatg aaagcagtct gaaagtggcc gaatggctgg cagaacatcc
    41581 gcaagttgcg cgagttaacc accctgccct gcctggcagt aaaggacacg aattctggaa
    41641 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcaatga
    41701 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    41761 cgggtatgaa tcgttgatcc tggcaaatca accagaacat atcgccgcca ttcgcccaca
    41821 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    41881 cgatctgatt gccgatctgg acgccggttt tgcacggatt gtataacatt gccacttttg
    41941 gacaattttg cagacatttt attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    42001 gtcccggacg attcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    42061 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    42121 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    42181 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    42241 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    42301 caagtctggg ctgctggcta agttatattc aggggagctg gttagggaat accaaaacgg
    42361 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    42421 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    42481 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    42541 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    42601 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    42661 tgttaaccgc tggcttgtta ggcacgctgt ttgtggtgat taaaaaaaaa tactgtaacg
    42721 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    42781 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    42841 ctaaattgcg atgcgctttc atagccgacg cgcatcgctg ctgcgctggc cttcatgccg
    42901 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    42961 gaggtgctgg tgacagactt aaaattatgg tggaacgccg atacgctcat gttggcttct
    43021 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaattcg tttcagcacg
    43081 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    43141 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    43201 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    43261 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    43321 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa ccctgccagc
    43381 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    43441 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    43501 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    43561 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    43621 ttaactttat ccgccagcaa gcgacaaatc tcttcacgtt tcatatcgcg taatttctta
    43681 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    43741 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    43801 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    43861 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    43921 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggtgg cggcagcgtg
    43981 aaaaaaaccg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    44041 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    44101 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    44161 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    44221 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    44281 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    44341 gcgttccatt ctgcacatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    44401 accctgccgc cgcgtcaggt ggctaacggc gtagtggacg cctttgtaca caccgtggaa
    44461 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    44521 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    44581 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcac tggcgtaccg
    44641 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    44701 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    44761 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    44821 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    44881 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    44941 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    45001 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    45061 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    45121 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    45181 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    45241 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    45301 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    45361 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    45421 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    45481 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    45541 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    45601 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcttgg aacgcgacac
    45661 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    45721 agaaagtcat tcgcgatctg gcagataaat acggcaaaac cccggcgcag attgttatcc
    45781 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    45841 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc aaaattgcaa
    45901 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacgtgcaa
    45961 attctcccgg tggcggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    46021 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    46081 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    46141 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    46201 cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat
    46261 aaatcagatg ccagtacgcc ttatttcggt gaaaccggag agcgggcgat aacttcagcg
    46321 cgtattcaac aggcgcttac cctgaacgac aatcttacac taccgggcaa tattggctgg
    46381 ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgtt agaaagcgta
    46441 gcgataaccc aaactgacgt cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa
    46501 gatgataaag aaaagaccac acagacactg aaaatggtgg cggaaaatgg tcgttgggtc
    46561 attgatgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa
    46621 aaaacgctgg ccgctttagc ttcgttgcag aaagaacagc cagaatcctt tgttgctgaa
    46681 ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac
    46741 cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa
    46801 gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg
    46861 ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt
    46921 catgttcgtt ttacgctgac caatggcaac aatgaagagc aagagctggt tttacagcgg
    46981 cgcgaaggta agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag
    47041 cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag
    47101 ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg
    47161 ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga
    47221 gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatatg aaagcactac
    47281 gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgcac
    47341 aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc
    47401 ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc
    47461 ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc
    47521 gtctgacgct gagtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc
    47581 tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg
    47641 ctgccaatca gatgcttttt gcccatcgct tccagcgcct ggcggattaa cggccagttt
    47701 gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt
    47761 acgaagacgc cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac
    47821 atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa
    47881 cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc
    47941 gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg
    48001 aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc
    48061 ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac
    48121 tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgataagg
    48181 atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg
    48241 ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt
    48301 tcagcgcgtg gcgatttgca gcgcaacata tacatgttgg cagttggccc accgagatcg
    48361 gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc
    48421 gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa
    48481 cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga
    48541 atacgggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg
    48601 tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg
    48661 tgtttttgca tcaatgcgcg ggcgcagcct gggttggttt cgtggtgcag aatgcgcgaa
    48721 gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag caatacgtag
    48781 gttttttccc acggtttctg gcgcggtggc tgcacgatta cagctttcgc ttcctgcttt
    48841 ttcggcgcaa caggtttgtt atccgcacac ggcaaatctt cgccatacgg atgcgggatt
    48901 gggtcgattt tccctagggt atcaagacgg gtggaatcca caccgctcca gccaggcagc
    48961 gcctctttta cgataattgc ggtattacgc acatcgcgga tttcactaat cggctcgccc
    49021 atcgccagac gatgcgctac ttccaccagc ggacgttcac cgttaccaaa catcagcatg
    49081 tcggctttcg aatctaccag aacggaacgg cgcacggtat cggaccagta atcataatgc
    49141 gcggtacggc gcaggctagc ctcaataccg ccgaggatca ccggcacatc tttccacgcc
    49201 tctttacaac gctgggtata aaccagcgtg gcgcgatccg gtcgcttacc cgcgacatta
    49261 tctggcgtgt aggcatcatc atgacgtaaa cggcgatcgg cggtatagcg gttgatcatc
    49321 gaatccatgt tgccagcagt aacaccaaaa aacagattcg gtttacccag acgcataaag
    49381 tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgcgcttcc
    49441 agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta
    49501 accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc
    49561 aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga
    49621 tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca
    49681 aaggccgggg attataagcc ggaacgaaag agaaatcgaa aggtattcca tactcgccct
    49741 cctcgggcga gtatgaagat tacggtaccg gattgaccaa cagttgccca atcgagccac
    49801 ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct
    49861 gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc
    49921 tgtcttccgg gaacggcatc gcgccattca cgttacggat ctggaacatt acgccttcaa
    49981 tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg
    50041 cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca
    50101 gactgatatc gcgactgcga attggcgaac cagccatgat ttcagttggc agcaagcgca
    50161 tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaagg
    50221 tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg
    50281 cttcaccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc
    50341 gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat
    50401 cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt
    50461 tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt
    50521 gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt
    50581 ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacac
    50641 cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg
    50701 caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag
    50761 tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc
    50821 gtgccggacc gcccatcagc gggcctggta cctgtagccc ggccaccgtc attgagacat
    50881 tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag
    50941 tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg
    51001 accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg
    51061 gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg
    51121 cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc
    51181 catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt
    51241 gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat
    51301 tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata
    51361 ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt
    51421 aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc
    51481 aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt
    51541 tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc
    51601 aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca
    51661 gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg
    51721 cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata
    51781 gatagcattg ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa
    51841 cagtggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg
    51901 gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat
    51961 aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac
    52021 gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta
    52081 atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa
    52141 tacgctgccg gatgacgact taacgtttca tccggcgttc cttgctagcg ggaggaaaca
    52201 gcgccctccc cggcatatta ctcttcgcta tcaccgctgc tggcacggcg aggagagtcg
    52261 atctcaacac gatcgatacg ctgcaaaccg cgcatcaacg taccgcggcg tccacgttcg
    52321 ccagtgactt tctgtagctc ttccggacgc agtttaattt tgcgtttccc aacatgaatg
    52381 gtcagcgtgc tttgcggcgg cagaacgtac agttgcgcca gaccatcctc gccacgcgcg
    52441 gcttctgccg atggaatgtt gataatcttg ttgcctttgc ccttcgacag ctgcggcaga
    52501 tcgcttaccg ggaacatcaa catacggcct gcctgagtga ttgccagcag catatcggaa
    52561 gcatcttcaa tcaccaccgg cggcataaca tgggcatttt ccggtaaggt gatcaaagcc
    52621 ttacccgcac ggttacgcgc taccagatcg ttaaaggtgc agacgaaacc gtaacccgca
    52681 tcggaagcca tcagcagttt ctgatcgtcg ctttccatca gcatatggtc aacggtcgcc
    52741 ccaggcggca acgttaattt gccggtgagt ggttcgccct gaccacgcgc cgacggcagc
    52801 gtaatcgggt cgatggcata gctacgaccg gtggaatcaa caaacactac cggttggtta
    52861 ctcttacctt tcaccgccgc tttgaagcta tcacccgctt tataattcag gcccggcgcg
    52921 tcgatatcat ggcctttagc gctgcgtacc cagcccatct gcgacagcac aatggtgaca
    52981 ggttcagacg gcagcatgtc gtgctcgctc atcgctttcg cttcttcgcg ttcctgcaac
    53041 ggcgaacgac gatcgtcacc gtaggcttgc gcgtctgcct gcagttcttt cttcagcagg
    53101 ttattcattt tacgctcgga agccaaaatg ccctgcaact ggtcgcgctc tttttccagc
    53161 tcactctgct caccgcgaat cttcatctct tccagtttgg caagatgacg cagtttcagt
    53221 tcgaggatcg cttccgcctg ggtttccgta aggccaaacc gcgacatcag cgccggtttc
    53281 ggttcatctt cgttacgaat gatctcaatc acttcgtcga tgttgagaaa cgccaccagc
    53341 aaaccttcca ggatatgcag gcgcttgagg actttctcca gacgatagtt cagtcggcgg
    53401 cgcacggtat cgcgacggaa caccagccat tcggagagga tttccagcag gtttttcacc
    53461 gccggacgac catccagacc gatcatattg aggttaatgc gatagctctt ttccagatcg
    53521 gtggtagcga agaggtggtt catcacctga tccatatcca cgcggttgga acgcggcaca
    53581 atcaccagac gggtcgggtt ctcgtggtca gattcatcgc gcagatcgtc aaccatcggc
    53641 agctttttgt tgcgcatttg cgcagcaatt tgctccagta cgcgcgcacc tgaaacctga
    53701 tgcggtaatg cgctgataac caccgcgcca tcttctttct tccacaccgc gcgcatacgc
    53761 actgaaccac gtccgttctc gtagatttta cgaatctcgg cgcgcgaagt gataatttcc
    53821 gcttcagtcg gataatccgg cccctgcacg atatccagca gctgatcgag cgtagttttc
    53881 ggctggtcga ttaatgcgat tgccgcctga gctacttcac gcaggttatg cggtggaata
    53941 tcggtcgcca tgccgacggc aataccggtg gtgccgttaa gcaaaatgtt tggcagacgg
    54001 gcaggtagca ttttcggctc ctgcaaagtg ccgtcgaagt ttggcaccca gtcagccgtc
    54061 ccctgcccca gctcgctcaa tagcagctcg gaatatttcg acaaccggga ttcggtgtaa
    54121 cgcattgccg cgaacgattt cggatcgtcc ggcgcgcccc agttcccctg accatcaacc
    54181 agcggataac ggtaagagaa cggctgcgcc atcaggacca tcgcttcata acaggcgcta
    54241 tcgccgtgcg gatggtattt acccagtacg tcaccgacgg tacgggccga ttttttaaat
    54301 ttggcgctgg cattcaggcc cagttcagac atcgcataca caatgcggcg ctgaacaggt
    54361 ttcagaccat caccaataaa cggcaacgca cggtccatga tcacgtacat ggagtagttt
    54421 aagtaggcgt tttccgtaaa ttcatgtagc gcaaggcgct ctgccatatc gctcattaat
    54481 tctgattcct caacttattc gccagccttc aaaggggcaa tattgccgga gatactacat
    54541 tatctgccgc gtcgagtcac aggagatcag cgtgaatagc gccgccggac gtgctccgac
    54601 ggcgataaag gcatcaatgt gccttgatat acaactcttt gctgtagact tgatcggcat
    54661 agagggcagc acaaggttgc gcccatgcca caccactcgg catgggggtc cgctccgggc
    54721 gattcgagaa gttgaggtta tgcgagtcca gggaagccta aagcatcaaa gtagtcgact
    54781 gtaagtatac ggttcagccc agtttagctg tgtctccacc agtgccccgc gcctcctgcc
    54841 ataagtgcgg cttgctcatg gatatccccg agacggctta cggcgcaccg gataataaat
    54901 ttcgcgtata catggtattc cttaccggag aaattataag cccaacaaca atacgcctgt
    54961 gacattgttc gctcaaactt atcgcgagtg atttggctca cactccggta aagattcccc
    55021 atcacgccaa cggcacataa acatccgttt ccagtcgctg ctctgtcacc ccttcggcaa
    55081 gattggtgta gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt gcaggcagcc
    55141 agtggcgaat aatgccccat atcgtgtgtg aaatatcgtc cagctcgcca acgtggcggg
    55201 ccacggcata acgtccaccg gtaagttcac cattgctcac accataacga ttatcgggaa
    55261 tcggttcgct aacgctgccg cagatatcaa agcgaaacgc ttccggtgcg gtggtggcag
    55321 gggcgtccca ggcgacgcca aaagtctggc tttggttaac aggagaaagc cccgtctctt
    55381 tacgccacat gataaatttc gccacactgt agttgagcaa ctcagggcta cagcggtgcg
    55441 gcaacatcgc cacagggatt gatgggaaat cgataatgtt tacatccagt gtcaggtttg
    55501 tcattttgcc tccctgcgaa attattcccg ggaacgcgtc ccgggagcgg taacaattac
    55561 ggatttactt tgcggatctg tttgacgtca atttcaacag aattccagtc tttatcgact
    55621 tcaccctgaa tctcaaccgt atctttcggc gtcaccgtca caccgttcca gcgtttgtga
    55681 tcgatatcaa cattgatagt accgctggca tctttaaaca cgtagagatc gtcagagatg
    55741 cgttcaacga tattgccgcg cagggttacc caggtgtcgt cacgcaggga ttttgcgctt
    55801 tctacagtcg ttacgctgcc gttcggcccc tgaaatcctc cggcctgact ttgcgttacc
    55861 gatgggccag aaaaaccgcc ctgctctgct gccatcaccg gtgcactgca cagggccatt
    55921 actgcgatta ctgctgcgaa ttttttcatg tttattactc cctttaatgt ctgtttccga
    55981 gcatttaaca agatagtcct taacaacttc ttaagggaaa aaaataaaat ttaatgctgt
    56041 acagagcgcg ttacaacacg gtttactggc agcaaatacg gttatcgcag ggatgaaaaa
    56101 atgcgaattt tactgataga agatgacatg ctgattggtg acggcatcaa aactggcctt
    56161 agtaaaatgg gttttagtgt cgactggttt acacaaggtc gtcagggaaa agaggcgctt
    56221 tatagcgcac cttatgatgc ggtgatcctg gatttaacct taccaggcat ggatggtcgc
    56281 gatattttgc gcgaatggcg agagaaaggt cagcgtgagc cggtactgaa cctgaccgcg
    56341 cgtgatgcgt tagcggaacg tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt
    56401 aaaccttttg cgttgataga agtcgccgcc aggctggagg ctctgatgcg ccgaaccaac
    56461 ggccaggcca gcaacgagct gcgccacggt aacgtcatgc tcgaccccgg caaacgtatc
    56521 gccacgctgg ctggcgaacc cttaacactg aaaccaaaag aatttgccct gctggaatta
    56581 ctgatgcgta acgctggtcg ggtactgccg cgcaaactga ttgaagagaa actgtatacc
    56641 tgggacgaag aggtcaccag taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa
    56701 ctcggtagtg attttattcg taccgtgcat ggtattggct acacattagg tgagaaatga
    56761 aatttaccca acgtcttagt ctgcgcgtca ggctgacgct aatcttttta attctggcct
    56821 cggtgacctg gctgctttcc agctttgtcg cctggaaaca aacaacggat aacgtcgatg
    56881 aattgttcga cacccaactg atgctgtttg ccaaacggtt aagtacgctc gatctcaacg
    56941 aaatcaacgc ggcggatcgc atggcacaga cgccaaataa attaaaacac ggtcatgttg
    57001 atgacgatgc gctgaccttt gccatcttta cccacgacgg cagaatggtc cttaatgatg
    57061 gcgataacgg agaagatatt ccctatagct atcaacggga aggttttgct gacgggcaac
    57121 tggtcggtga agacgatcct tggcgttttg tctggatgac ctcacctgat ggcaaatatc
    57181 gcatcgttgt tggccaggaa tgggaatacc gtgaagacat ggcgctggcg attgttgccg
    57241 ggcaattgat cccgtggctg gtcgcactgc cgattatgtt aatcatcatg atggtactac
    57301 tgggtcgtga actcgcgccg ctgaacaaac tggcgctggc actacgtatg cgtgaccctg
    57361 actcggaaaa accactaaac gcgactggcg tacccagcga agtgcggcca ctggttgagt
    57421 cgctaaatca actgttcgcc cgcacacatg cgatgatggt tcgagaacga cgctttacct
    57481 ccgatgcagc tcacgaacta cgtagcccgt taacagcgct gaaagtgcaa accgaagttg
    57541 cgcagctctc tgacgatgat ccgcaggcgc ggaaaaaagc actgctccaa ttacattccg
    57601 ggatcgatcg cgctactcgt ctggttgatc aactgctcac gctatcgcgg ctggactcac
    57661 tggataacct tcaggacgtc gcggagatcc cgcttgaaga tctcctacaa tcgtcggtga
    57721 tggatattta ccacacggcg cagcaggcga aaattgacgt gcgactgaca ctcaatgccc
    57781 acggcatcaa acgcaccgga caaccgctat tgctaagttt gttggtgcga aatttgctgg
    57841 ataacggccc cggtgtgaca ccagaggcac tggcgcgaat tggcgaacgc ttctatcgcc
    57901 cacccggaca aaccgctacc ggtagtggac ttgggctatc gattgtccag cgaatcgcca
    57961 agctgcatga catgaatgtt gagtttggga atgcggaaca aggtggattt gaggcgaagg
    58021 taagctggta acttctctgt ctatgagagc cgttaaaacg actctcatag attttactaa
    58081 tagcaaaata taaaccgtcc ccaaaaaagc caccaaccac aaaccagaca aataacgagc
    58141 gagaatacat atagccaaaa ctcgaacatt tcccgcataa agagttttct taagataaga
    58201 ataataagtg gcgtaagaag aaaaaatgct gcaatcattg cttgcaatat acgatcttcg
    58261 cctgaaacgg aaggtatact gcaaatattg cctcccgact caagcatcca gtcatactta
    58321 ttgaaagcaa aaaaagaatc tattaaccaa taagtaagca ttatatatgg caatgcttca
    58381 aatattgttt taattttctg ctttaacata tatcagtacg ctcataaaaa tccatttata
    58441 attatcaaca tgacacgttg atattttcat cttttactta ttcaaagcca tattttcaat
    58501 ccccaaaaaa accgaaccaa acgtgtaaat tattccggtg cgagtaagaa ttatgaggaa
    58561 tggctatcag tattgtcatt ttcagaaaat atttatcctg catcggtgag tcagagctag
    58621 atcagacttt tgctaaattc gcaaaagact ttgcacattt tgctaatttc acagtaccgc
    58681 tctgtgacgt actatagtcg acaaacgtct caccttgaga ttaaaaaatg agcaacatcc
    58741 tgattatcaa cggcgcgaaa aaattcgccc actccaatgg tcaactgaac gacaccctga
    58801 ccgaagtcgc ggatggcaca ctgcgcgacc tcgggcatga tgtccgcatc gttcgcgccg
    58861 acagcgacta caatgtcaaa gcggaagtac aaaactttct ctgggctgat gtggtgatct
    58921 ggcagatgcc aggctggtgg atgggcgcgc cgtggacagt gaaaaaatat attgatgatg
    58981 tattcaccga aggtcacggg actctgtatg ccagcgatgg tcgtacccgc aaagatccgt
    59041 cgaaaaaata cggttccggc agcctggtac aggacaaaaa atatatgctt tctctaacct
    59101 ggaacgcacc aatggaagcc ttcaccgaaa aagatcagtt cttccacggc gttggcgttg
    59161 acggtgtgta tctgccgttc cataaagcaa accaattcct cggtatggaa ccgctgccga
    59221 catttatcgc taatgacgtg ataaaaatgc ctgatgttcc ccgctatact gaagaatatc
    59281 gcaagcatct tgtggaaatt tttggttaac tagagctcag gctttagaag gagttaacca
    59341 tgcttaccgt aatcgcagaa atccgtactc gtcctggtca acatcaccgt caggcggtat
    59401 tggatcagtt tgctaaaatc gttccaaccg tactgaaaga agaaggttgc cacggctatg
    59461 cgccaatggt ggattgcgct gctggcgtga gtttccagtc tatggcaccg gattctatcg
    59521 tgatgattga gcagtgggaa agcatcgcgc accttgaagc gcatctgcaa accccgcaca
    59581 tgaaggcgta cagcgaagcc gtaaaaggcg acgtgctgga gatgaatatc cgtattctgc
    59641 agccagggat ttaatcctgc cttgtttgcc cggccatcct gaccgggcaa tgttctttcc
    59701 tttaaacctc aatctccgcc atgtcgcctt tctcttgcaa ccagttgcgg cgatcttccg
    59761 agcgtttctt cgccagcagc atatccatca tcgcgtcagt acgctgatcg tcttcatcat
    59821 cgatagtcaa ctgcaccaga cggcgagtgt tcggatcaag cgtggtttca cgcaattgca
    59881 tcgggttcat ttcccccaga cctttaaaac gctggacgtt cggcttgcct ttcttgcgtt
    59941 ttaattgctc aagtacgccc tctttctctt cttccgtcag cgcgtaataa acctctttcc
    60001 cgagatcaat acggtagagc ggtggcagtg cgacgtaaac gtgaccgtgt ttcaccaacg
    60061 cgcggaagtg ttttacgaac aaagcgcaga gcagcgtggc aatgtgcaga ccatcagagt
    60121 ccgcatccgc gaggatacag attttgccat aacgaagctg gctcagatcg tcgctgtcag
    60181 gatcgatacc gatcgctacc gaaatatcat gcacttcctg cgaggccagc acttcgtcgg
    60241 aagaaacttc ccaggtgtta aggatcttac ctttcagcgg catgatcgcc tgatattcac
    60301 gatcgcgcgc ctgcttggca gatccgcctg cggagtcacc ttccacgagg aacagttcgg
    60361 tacggttaag gccctgcgcg gtacaatcag ccagtttgcc aggcagcgcc gggccgctgg
    60421 tcagcttttt acgcaccact tttttagccg cacgcatacg acgctgggcg ctggaaatcg
    60481 ccatctctgc cagcaactcc gccgcctgaa cgttctggtt cagccacagg atgaaggcat
    60541 ctttcaccac gcccgaaacg aatgccgcac actgacgcga agagagacgc tctttggtct
    60601 gcccggcaaa ctgcggatcc tgcattttaa ccgacagaac ataggcgcag cgatcccaga
    60661 tatcttccgc cgacagcttc acgccgcgcg gcagaatgtt gcggtattca cagaactcac
    60721 gcatcgcgtc caacaggccc tgacgcagac cattaacatg ggtaccgccc tgcatcgttg
    60781 ggataaggtt gacgtagctt tcggtcagca gttcaccgcc ttccggcagc cacagtagcg
    60841 cccagtccac agcttcagta tcgccagcga aattaccgat aaacggtttt tctggcagcg
    60901 tcggtaaacc gtttaccgct tccgccaggt aatcattcag accgtcctga tagcaccagc
    60961 gctgttcggt gttgttgatc tcatctttaa aggtaatctc aacgccgggg cacagtactg
    61021 ctttggcctt cagcacatgc gtcaggcgtg aaacagaaaa acgcgggctg tcaaagaagg
    61081 tttcatccgg ccagaagtgc acgctggtac cggtattgcg tttaccgcaa gtgccgacaa
    61141 cctgtaaatc ctgcaccttt tcgccatttt caaaggcgat gttataaacc tgaccatcgc
    61201 ggcgcacgtt aacttctacg cgcttcgaca aggcgttaac taccgaaatc cccacgccat
    61261 gcaggccgcc agagaactgg taatttttgt tagagaattt accgcctgca tgcagacggc
    61321 aaagaatcag ttcaaccgcc ggtactcctt cttccgggtg aatatccacc ggcatcccgc
    61381 gcccatcgtc aatcacttct aacgactggt cagcatgtaa gattacatcc acgcgttttg
    61441 cgtgacccgc cagtgcttca tcgacactgt tatcaataac ttcttgcccc aaatggttag
    61501 ggcgagtggt atcggtatac atccccggac ggcggcgaac cggctcaagc ccggtgagta
    61561 cctcaatggc atcagcgtta taagtttgcg tcatggttta agttagcaat tcgagttgat
    61621 cgtcagagat ggtgcagacc aagaaaatcg acgatcgggt tgaaataatc ttcgaagccc
    61681 gtgaatgcgt ggttgccgcc ttctatgaca gtctggcggc aggaagcata gtacgccacc
    61741 gcctggcggt aatccagcac ttcatctccc gtctgttgca gcagccagat caaatccggc
    61801 gcttccagcg ggtcaatctg catgactttg agatcgtaaa tatggcgtga ctctagcaca
    61861 tattgctgcc cggtgtaggg gttctcgttc tgaccgagat agtccgtcag caattcaaac
    61921 gggcgtaccg ccgggtttac caccactgcg ggcagcataa aacattgcga caaccaggtg
    61981 gcgtaatatc cccccagtga cgaaccgaca atacccagcg aatcgccgcc atgttccagg
    62041 acaatggatt ccagcagttc tgccgcgtcg gaaggatacg gcggcaactg cggaatgatc
    62101 atctcaacgt cagggtgatg ttccgccagc cagtttttta acaagctcgc ttttgcagag
    62161 cgcggcgagc tgttgaaacc gtgtaaataa agaagcgtag acatcagtag ccttctgaag
    62221 cggtatcagg ttggaaacgt gtgtccgcca ggcgatgcac ctcggtggtc agcgtgccat
    62281 cagcatgtaa ctcgagagta cgccagccgg gcgcgatagt atccagcgta aagttggaac
    62341 agtgcggctt aaactgcaca caggtcgacg gcgttgccag caggcggcga ccgttccagt
    62401 cgagatccag ctcctgatga atatgaccgc acagcaagta tttgacgtgc ggaaacttcg
    62461 ccagcacggt atccagttcg cccgcgttac gcagactgtg ttgatcgagc caactacaac
    62521 ccgcaggtag cggatgatga tgcagcagca gcaacgtatg gcgttctggc gcatcggcca
    62581 gtttacgttc cagccactca agctgaaact cgctcagctc accgtgcggc acgccaaaca
    62641 cctggctatc cagcaacagg atttgccatt gctcaccaat aaacacccgc ttcgccgggg
    62701 agatacccgc atcctgtaac gcgctgtaca tcgcgggctg gaaatcgtgg ttgcccggca
    62761 gccagacgca gggcgcacga aaacttgcga tgccttcagc gaaatgctga taggccgcag
    62821 aggattgatc ctgcgctaaa tcacctgtcg cgacaatcag gtcgaattcg tgctggtgtg
    62881 gacgaatcgc ctccagcacc gcctggtagc tttcccaggt gtttacccct aacagggctt
    62941 cgtgcttttg tgcaaacagg tgagtatcgg taatttgtaa aatccttcct ctggcctcac
    63001 cagccagagg aagggttaac aggctttcca aatggtgtcc ttaggtttca cgacgctagt
    63061 aaaccggaat cgccatcgct ccatgtgcta aacagtatcg caaccagtcc gctaaaaact
    63121 ggttaatttg atgcttttcg tcgcgttgat gcaacttttt attaggataa tcataccgcg
    63181 ctttgaagcg aaaaatctgc tggcttgaac acacttcagc caccatcgcg tcatgataca
    63241 gacgcaccgt cattgacgga aggctccagt aactgatcgc gggcgcagtc tgttctattg
    63301 tcaccagggt agtgtatcgg gtcgattcca caatcgtcag ccgatattgt gcgtttgcca
    63361 cctgatagct tacagtttcg ccgggtgcgt cattgcgcgg taacaaacgg cgcaattgtg
    63421 aaaagttcat ctcgcacagg cgcatcattt caggaaagtc aggtgtgtaa cgcttcattt
    63481 atgcccactc attttttaac gcttgatgat gcagctgcag ccattgcaaa gcgatgaccg
    63541 acgctgcgtt gtcgattttc ccctcttcta cccactggta tgcctgttcc cggcttacca
    63601 catgaacgcg aatatcttcg ttttcatcag ccagaccatg aataccgctt gcggtcgtgg
    63661 cgtccacttc gcccaccata attgacgaac gctcactggt gccccccggg cttgccagga
    63721 aacttaacac cggtttggtc cgtttgacta tcagtcccgc ctcttcaatc gcttcgcgac
    63781 gggcaacatc ttccacactt tcaccctctt caatcatccc ggcaaccatc tccagtagcc
    63841 agggggtttc gctggtgtcg tacgcggcaa tccgaatctg ctcaatcagc acaacttcat
    63901 cacgcactgg gtcaaagggt agcaagactg cggcgtgacc gcgctcaaaa atttcccgcc
    63961 gtacctcatg actcatttgc ccgttgaata gacgatgacg aaatctataa agatctaatg
    64021 aaaaaaagcc gcgataaagt gtttctcgtg caataatttc tacatcgttt ttgccaaatg
    64081 taacgggcag gttgtctggc ttaagcattg ttaatgtcct agcactaata gtgaattaaa
    64141 tgtgaatttc agcgacgttt gactgccgtt tgagcagtca tatgttaaat tgaggcacat
    64201 taacgcccta tggcacgtaa cgccaacctt ttgcggtagc ggcttctgct agaatccgca
    64261 ataattttac agtttgatcg cgctaaatac tgcttcacca caaggaatgc aaatgaagaa
    64321 attgctcccc attcttatcg gcctgagcct ttctgggttc agttcgttga gccaggccga
    64381 gaacctgatg caagtttatc agcaagcacg ccttagtaac ccggaattgc gtaagtctgc
    64441 cgccgatcgt gatgctgcct ttgaaaaaat taatgaagcg cgcagtccat tactgccaca
    64501 gctaggttta ggtgcagatt acacctatag caacggctac cgcgacgcga acggcatcaa
    64561 ctcgaacgcg accagtgcgt ccctgcagtt aactcaatcc atttttgata tgtcgaaatg
    64621 gcgtgcgtta acgctgcagg aaaaagcagc agggattcag gacgtcacat atcagaccga
    64681 tcagcaaacc ttgatcctca acaccgcgac cgcttatttc aacgtgttga atgctattga
    64741 cgttctttcc tatacacagg cacaaaaaga agcgatctac cgtcaattag atcaaaccac
    64801 ccaacgtttt aacgtgggcc tggtagcgat caccgacgtg cagaacgccc gcgcgcagta
    64861 cgataccgtg ctggcgaacg aagtgaccgc acgtaataac cttgataacg cggtagagca
    64921 gctgcgccag atcaccggta actactatcc ggaactggcg gcgctgaatg tcgaaaactt
    64981 taaaaccgac aaaccacagc cggttaacgc gctgctgaaa gaagccgaaa aacgcaacct
    65041 gtcgctgtta caggcacgct tgagccagga cctggagcgc gagcaaattc gccaggcgca
    65101 ggatggtcac ttaccgactc tggatttaac ggcttctacc gggatttctg acacctctta
    65161 tagcggttcg aaaacccgtg gtgccgctgg tacccagtat gacgatagca atatgggcca
    65221 gaacaaagtt ggcctgagct tctcgctgcc gatttatcag ggcggaatgg ttaactcgca
    65281 ggtgaaacag gcacagtaca actttgttgg tgccagtgag caactggaaa gcgcacatcg
    65341 tagcgtcgtg caaaccgtac gttcctcctt caacaacatt aatgcttcta tcagtagtat
    65401 taacgcctac aaacaagccg tagtttccgc gcaaagctca ttagacgcga tggaagcggg
    65461 ctactcggtc ggtacgcgta ccattgttga tgtgttggat gcgaccacca cgctgtacaa
    65521 cgccaagcaa gagctggcga atgcgcgtta taactacctg attaatcagc tgaatattaa
    65581 gtcagccctg ggtacgttga acgagcagga tttgctggca ctgaacaatg cgctgagcaa
    65641 accggtttcc actaatccgg aaaacgttgc cccgcaaacg ccggaacaga atgctattgc
    65701 tgatggttat gcgcctgata gcccggcacc cgtcgttcag caaacatccg cacgcactac
    65761 caccagtaac ggtcataacc ctttccgtaa ctgatgacga cgacggggct tcggccccgt
    65821 ctgaacgtaa ggcaacgtaa agatacgggt tatctgccgc attcttcccc cttctcgctt
    65881 caatttcgac cagccatcct ctattctgat gggtatttac cactggtccc ggaagacaaa
    65941 aatgaaacgg acaaaatcca tacgccacgc atcgttccgc aaaaactgga gcgcacgcca
    66001 tctgacacca gtcgctctcg cggttgccac tgtttttatg ctggctggct gtgaaaagag
    66061 tgatgaaaca gtgtctctct atcaaaatgc tgacgactgt tcagctgcaa acccaggcaa
    66121 aagcgccgaa tgtaccaccg cgtacaacaa tgcgctgaaa gaagccgaac gtactgcgcc
    66181 gaaatacgcc acccgtgaag actgtgttgc tgaatttggt gaaggtcagt gtcagcaagc
    66241 accagcccag gctggcatgg caccagaaaa ccaggcgcag gctcagcaat ccagcgggag
    66301 tttctggatg ccgctgatgg ccggttacat gatggggcgt ctgatgggcg gcggcgcggg
    66361 atttgcacag cagccgctgt tctcctcgaa aaacccggcc agtccggctt acggtaaata
    66421 taccgacgcg acgggtaaaa actatggcgc agcccagcca ggccgcacca tgaccgtacc
    66481 gaagacggca atggcaccaa aaccggcgac caccactacc gttacccgtg gcggttttgg
    66541 tgaatctgtt gccaaacaaa gcactatgca gcgtagcgca accggcacct cttctcgttc
    66601 aatgggtggc tgataccgat ggaaagagtc agtattaccg agcgcccgga ctggcgtgag
    66661 aaagcccacg aatacggttt caattttcac accatgtacg gcgagccgta ctggtgtgaa
    66721 gatgcttact acaagttgac cctcgcccag gttgaaaagc tggaagaagt caccgccgaa
    66781 ctgcaccaga tgtgcctgaa agtggtggaa aaagtgatcg ccagcgatga gctgatgacc
    66841 aaattccgca taccaaaaca cacctggagt tttgtgcgtc aatcatggct gactcaccag
    66901 ccatcgcttt attcgcgtct tgatctggcg tgggatggca ctggcgaacc taaacttctg
    66961 gaaaataacg ccgatacgcc aacgtcacta tacgaggcgg cgttctttca gtggatctgg
    67021 ctggaagatc agcttaacgc cggtaacttg ccggagggca gcgaccagtt taacagtctg
    67081 caagaaaaac tgatcgatcg cttcgttgag ctgcgtgaac agtatggctt ccagttgctg
    67141 catctcacct gctgtcgcga cacggtggaa gatcgcggaa ccattcagta tttgcaggac
    67201 tgcgcaaccg aagctgaaat cgccaccgag ttcctctaca tcgacgagat aggtttaggc
    67261 gaaaaaggcc agttcacgga tttacaggat caggtgattt ctaacctgtt caagctttat
    67321 ccgtgggaat ttatgctgcg cgaaatgttc tccaccaagc tggaagatac tggcgtacgc
    67381 tggctggaac cggcgtggaa gagcattatc tccaacaagg cacttctacc gctactgtgg
    67441 gagatgttcc cgaatcaccc gaacctgctg cccgcttatt ttgcggaaga tgatcatccg
    67501 caaatggaaa aatatgtggt taaaccgatc ttctcccgtg aaggcgcaaa cgtgtcgatc
    67561 attgagaacg gcaaaaccat tgaagcagcg gaaggtccgt atggcgaaga agggatgatt
    67621 gttcagcaat tccacccgtt accgaaattc ggcgacagct atatgctgat tggtagctgg
    67681 ctggtgaacg atcaacctgc cggaattggc attcgtgaag accgtgcgtt gatcacccag
    67741 gatatgtctc ggttttatcc acatattttt gttgaataag ccacgatacc ggatggcact
    67801 cgccatccgg taattgttag cctatctgca ccgacagcat acttaaactg cccatttcta
    67861 taccatcgac cggaatggta attggctcct gcccatccca cacacctaac acatacaaca
    67921 acggcagata gtgctctggc gttgggttcg ataacgcgcc accttcatgg tcgagataat
    67981 tcaccagagg atgctgctct accggccctt gccacgtcag attcgctttc acatactcat
    68041 taaacgacgt cgcccacgga tacggtgaac tatcaccgtg ccacttcact gtgcgcaggt
    68101 tatgcaccac gttaccgctg gcgaccaaca ttattccttc atcacgcagc gccgcgagtt
    68161 tgcgccccat ttcgaaatgc caggcggcag gtttgctact gtcgatactc aactgcacca
    68221 tagggatatc ggcgtcagga tacatcttaa tcagcacgcc ccacgagccg tggtcaaagc
    68281 cccaggcttc tttatccagc gtcaccggga tcggcgctaa cagctcaacc agacgctgtg
    68341 ccagcgcagg cgaacccgga gcaggataat gcgtatcgta cagcgcctgc gggaagccac
    68401 caaagtcatg aatcgtgggc ggcgtctcca tcgcggtcac tcctgttcca cgggtaaacc
    68461 agtgagccga aaccaccaca atcgcttgcg ggcgtggcaa tgtcatcccc aacttctgcc
    68521 agctgcgggt atacaaatta tcttccagca cgttcatcgg actaccgtga cctaaaaaca
    68581 atgctggcat acgtgttgaa gacatgatga tatccttaac taaaggtgtc attttgatat
    68641 cctcacaata cgcttgttcg gcggagtaag aacccggata acaatgatga tgatcatcag
    68701 ttattttgac gatctgcctg aaggtgaaga tttataagga gttgtcgatg tcagtacctc
    68761 tcattctgac catactggcg ggggcagcca cgtttattgg cgcgtttctc ggcgttctcg
    68821 gacaaaaacc ctcgaaccgc ttactggcgt tttcgctagg ttttgcggcg gggatcatgt
    68881 tgctcatctc attaatggaa atgcttcctg ccgcactagc agctgaagga atgtcgccgg
    68941 tgttgggtta tggaatgttt atcttcggtc tgcttggcta ctttggcctg gaccgcatgt
    69001 tgccgcatgc gcatccgcag gatttaatgc aaaaatcggt gcagctgttg ccaaaatcga
    69061 tcaagcgcac agccattctg ctcactctcg gcatcagtct gcataacttc ccggaaggga
    69121 ttgccacctt tgtcacggcg agcagcaacc tggagctggg atttggcatc gcactggccg
    69181 tcgcgttgca caatatccct gaaggtctgg cagtggcagg cccggtttat gcggcaacgg
    69241 ggtctaaacg caccgcgatt ctgtgggcgg ggatttctgg actagcagaa attcttggtg
    69301 gtgtgctggc gtggttaatc ctcggtagca tgatttcccc ggtggtcatg gcggcaatca
    69361 tggcggcggt tgcaggaatt atggtggcgc tctcggttga tgaattaatg ccgctcgcca
    69421 aagagattga ccctaataat aaccccagct atggcgtact atgtggaatg tcagtgatgg
    69481 gattcagttt agtgctgcta caaacggcgg gaattggtta aaaacaacat cagaagttgt
    69541 ccagcacatg gacaacttct gccagcttca cgaaagcctt attctacctt ttcggctgcc
    69601 tcttctctcg tactgtttaa gtatttgttc cgcgcttcaa gcagtgcgtc tctgtcctgg
    69661 cgaaacgccc gacaataacg tttcaaatgc aggaaataac ccaccacaac cagagcaatc
    69721 aatacaatcc agtaccaggc gataaacatc cttttaccct taatatttaa caatactaaa
    69781 catttagcgc ataaatttca catatccttt ttcggatata ttcatatggt cgcataataa
    69841 cggaacaatt atatgatttt gatcataatg aaaaagaatg cctgatatta atgaaatttt
    69901 ctcatagaaa gagaagcgga attaacatct tgcatagcag ggcaaaataa aaaccgaagc
    69961 cagctacaac ttcggtttct tgattaaggc agtaattaag cagcggtttt cagctggctt
    70021 tacgctcatg tgcctgacgg tatgccacca ggtcttcaat agtcacgagc gccatattgt
    70081 gtttattggc aaactcaatg cactctggtg cacgcgccat cgtgccatcg tcattagtca
    70141 gctcacacag cacaccagcc ggtttaaagc ctgccagcgt catcaaatca atagtcgctt
    70201 cagtatgacc gccacgcgtc agtacaccac ctgcctgcgc gcgaagtggg aaaacgtggc
    70261 ccgggcgatt cagatcagaa ggttttgcgc catcggcaat cgctgcgcga acggtcgtaa
    70321 tacgatcagc ggcagaaaca ccggtagtca caccttcagc cgcttcaatg gtcacggtaa
    70381 aaccggtgcc ataggcgctg gtgttatttt ctaccatcat tggcagatca agttgtttac
    70441 ggcgatcttc agtgatgcac aggcaaacaa taccgctacc gtggcgaatg gtcagcgcca
    70501 tctgctcaac ggtcatggtt tctgccggga agatcatatc gccttcgttt tcacggtctt
    70561 catcatcaag caccattaca ccgcgtcctt cacgcagcgc agccagtgca ttttcaacac
    70621 gttcgaaagg cgtaccaaaa gaggaaagta gcgtctgatt catggtaaaa aaacctcact
    70681 aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag ccaaaataac
    70741 gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc taaccgtcgg
    70801 ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc tcgcgggctt
    70861 tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa taagcggatt
    70921 cactataacg ctaatgatta gcggcagcaa cgcatagctt cacataattc tggtttatga
    70981 cttaccctta tcgcactaca atggtactca acacctatca gtacagggaa actacaatga
    71041 ttgacccgaa aaaaattgag caaatcgctc gccaggttca cgaatcaatg cctaaaggaa
    71101 tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa gctcagctga
    71161 cgcgcctcga ccttgtaagc cgtgaagagt tcgacgtcca aacgcaagtc ctgttacgga
    71221 ctcgtgagaa actggcgctg cttgaacaaa gaatcagcga actggaaaat cgcagtactg
    71281 agatcaaaaa gcagccagaa ccagagactc tccccccaac gctgtaaaca tttgtcttta
    71341 atgcactgta aattatgaat taatcctcta atttacagtg ttttctatta cattatcgct
    71401 taatcccatt cagagtatca tcacaaaaat gaatgattgc aattgaaaat ataattgtta
    71461 tgtattcttt aagttttact taatcgata
//
LOCUS       DAJTRH010000006        70077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTRH010000006 DAJTRH010000000
VERSION     DAJTRH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..795
                     /locus_tag="OO339_000457"
     CDS             <1..795
                     /locus_tag="OO339_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5925207.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="OO339_000458"
     CDS             806..1897
                     /locus_tag="OO339_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5925208.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO339_000459"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO339_000459"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5925209.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="OO339_000460"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="OO339_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5925210.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="OO339_000461"
     CDS             4859..5254
                     /locus_tag="OO339_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5925211.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="OO339_000462"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="OO339_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5925212.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="OO339_000463"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="OO339_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5925213.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="OO339_000464"
     CDS             complement(6563..7603)
                     /locus_tag="OO339_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5925214.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="OO339_000465"
     CDS             complement(7676..8311)
                     /locus_tag="OO339_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5925215.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="OO339_000466"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="OO339_000466"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5925216.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="OO339_000467"
     CDS             complement(8937..9380)
                     /locus_tag="OO339_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5925217.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO339_000468"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO339_000468"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5925218.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="OO339_000469"
     CDS             complement(9720..10223)
                     /locus_tag="OO339_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5925219.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="OO339_000470"
     CDS             complement(10217..10741)
                     /locus_tag="OO339_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5925220.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="OO339_000471"
     CDS             10950..11945
                     /locus_tag="OO339_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5925221.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="OO339_000472"
     CDS             11954..12832
                     /locus_tag="OO339_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5925222.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="OO339_000473"
     CDS             12913..13920
                     /locus_tag="OO339_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5925223.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO339_000474"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO339_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5925224.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO339_000475"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO339_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5925225.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO339_000476"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO339_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5925226.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO339_000477"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO339_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5925227.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO339_000478"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO339_000478"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5925228.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO339_000479"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO339_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5925229.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO339_000480"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO339_000480"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5925230.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO339_000481"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO339_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5925231.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OO339_000482"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OO339_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5925232.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OO339_000483"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OO339_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5925233.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OO339_000484"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OO339_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5925234.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="OO339_000485"
     tRNA            complement(27654..27730)
                     /locus_tag="OO339_000485"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="OO339_000486"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="OO339_000486"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5925235.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="OO339_000487"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="OO339_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="OO339_000488"
     tRNA            complement(31514..31600)
                     /locus_tag="OO339_000488"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OO339_000489"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OO339_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5925236.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OO339_000490"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OO339_000490"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5925237.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OO339_000491"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OO339_000491"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5925238.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OO339_000492"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OO339_000492"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5925239.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OO339_000493"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OO339_000493"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5925240.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="OO339_000494"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="OO339_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5925241.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OO339_000495"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OO339_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5925242.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="OO339_000496"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="OO339_000496"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5925243.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OO339_000497"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OO339_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5925244.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="OO339_000498"
     CDS             complement(41212..42177)
                     /locus_tag="OO339_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5925245.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OO339_000499"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OO339_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5925246.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OO339_000500"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OO339_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5925247.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="OO339_000501"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="OO339_000501"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5925248.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="OO339_000502"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="OO339_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5925249.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OO339_000503"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OO339_000503"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5925250.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OO339_000504"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OO339_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5925251.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OO339_000505"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OO339_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5925252.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OO339_000506"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OO339_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5925253.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OO339_000507"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OO339_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5925254.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OO339_000508"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OO339_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5925255.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OO339_000509"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OO339_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5925256.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="OO339_000510"
     CDS             49715..50680
                     /locus_tag="OO339_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5925257.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="OO339_000511"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="OO339_000511"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5925258.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="OO339_000512"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="OO339_000512"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5925259.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="OO339_000513"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="OO339_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5925260.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="OO339_000514"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="OO339_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5925261.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="OO339_000515"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="OO339_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5925262.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="OO339_000516"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="OO339_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5925263.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="OO339_000517"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="OO339_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5925264.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="OO339_000518"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="OO339_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5925265.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="OO339_000519"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="OO339_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5925266.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="OO339_000520"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="OO339_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5925267.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="OO339_000521"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="OO339_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5925268.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="OO339_000522"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="OO339_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="OO339_000523"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="OO339_000523"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5925269.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="OO339_000524"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="OO339_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5925270.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="OO339_000525"
     CDS             complement(62547..63476)
                     /locus_tag="OO339_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5925271.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="OO339_000526"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="OO339_000526"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5925272.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="OO339_000527"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="OO339_000527"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5925273.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
BASE COUNT        17138 a        19034 c        17411 g        16494 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtc
//
LOCUS       DAJTRH010000007        67520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTRH010000007 DAJTRH010000000
VERSION     DAJTRH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..228
                     /locus_tag="OO339_000528"
     CDS             <1..228
                     /locus_tag="OO339_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5925274.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="OO339_000529"
                     /pseudo
     CDS             623..1262
                     /locus_tag="OO339_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="OO339_000530"
     CDS             1365..1688
                     /locus_tag="OO339_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5925275.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO339_000531"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO339_000531"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5925276.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO339_000532"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO339_000532"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5925277.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO339_000533"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO339_000533"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5925278.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO339_000534"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO339_000534"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5925279.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO339_000535"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO339_000535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5925280.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO339_000536"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO339_000536"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5925281.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO339_000537"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO339_000537"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5925282.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO339_000538"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO339_000538"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5925283.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO339_000539"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO339_000539"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5925284.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO339_000540"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO339_000540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5925285.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO339_000541"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO339_000541"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5925286.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO339_000542"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO339_000542"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5925287.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO339_000543"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO339_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5925288.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO339_000544"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO339_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5925289.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /gene="alaA"
                     /locus_tag="OO339_000545"
     CDS             19267..20484
                     /gene="alaA"
                     /locus_tag="OO339_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5925290.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="OO339_000546"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="OO339_000546"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5925291.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="OO339_000547"
     CDS             complement(21226..23058)
                     /locus_tag="OO339_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5925292.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO339_000548"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO339_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5925293.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="OO339_000549"
     CDS             complement(23806..24300)
                     /locus_tag="OO339_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5925294.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="OO339_000550"
     CDS             complement(24383..24838)
                     /locus_tag="OO339_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5925295.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="OO339_000551"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="OO339_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5925296.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="OO339_000552"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="OO339_000552"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5925297.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="OO339_000553"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="OO339_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5925298.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO339_000554"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO339_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5925299.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO339_000555"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO339_000555"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5925300.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO339_000556"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO339_000556"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5925301.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="OO339_000557"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="OO339_000557"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5925302.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="OO339_000558"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="OO339_000558"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5925303.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="OO339_000559"
     CDS             33414..34307
                     /locus_tag="OO339_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5925304.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO339_000560"
     CDS             complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO339_000560"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5925305.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO339_000561"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO339_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5925306.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="OO339_000562"
     CDS             complement(36223..36939)
                     /locus_tag="OO339_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5925307.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO339_000563"
     CDS             complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO339_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5925308.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO339_000564"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO339_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5925309.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO339_000565"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO339_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5925310.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO339_000566"
     CDS             complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO339_000566"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5925311.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO339_000567"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO339_000567"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5925312.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO339_000568"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO339_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5925313.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO339_000569"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO339_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5925314.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO339_000570"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO339_000570"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5925315.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO339_000571"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO339_000571"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5925316.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="OO339_000572"
     CDS             complement(45788..46447)
                     /locus_tag="OO339_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5925317.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO339_000573"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO339_000573"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5925318.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="OO339_000574"
     CDS             complement(47342..48355)
                     /locus_tag="OO339_000574"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5925319.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO339_000575"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO339_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5925320.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /gene="flk"
                     /locus_tag="OO339_000576"
     CDS             49656..50651
                     /gene="flk"
                     /locus_tag="OO339_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5925321.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="OO339_000577"
     CDS             complement(50648..51826)
                     /locus_tag="OO339_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5925322.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO339_000578"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO339_000578"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5925323.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="OO339_000579"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="OO339_000579"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5925324.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="OO339_000580"
     CDS             complement(55608..55886)
                     /locus_tag="OO339_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5925325.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO339_000581"
     CDS             complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO339_000581"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5925326.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="OO339_000582"
     CDS             complement(56468..57277)
                     /locus_tag="OO339_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5925327.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO339_000583"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO339_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5925328.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO339_000584"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO339_000584"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5925329.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO339_000585"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO339_000585"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5925330.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="OO339_000586"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="OO339_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5925331.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="OO339_000587"
     CDS             complement(60978..61868)
                     /locus_tag="OO339_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5925332.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="OO339_000588"
     CDS             complement(61855..62379)
                     /locus_tag="OO339_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5925333.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="OO339_000589"
     CDS             complement(62376..62846)
                     /locus_tag="OO339_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5925334.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..63391)
                     /locus_tag="OO339_000590"
     CDS             complement(62843..63391)
                     /locus_tag="OO339_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5925335.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63396..64118)
                     /locus_tag="OO339_000591"
                     /pseudo
     CDS             complement(63396..64118)
                     /locus_tag="OO339_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64138..64860)
                     /locus_tag="OO339_000592"
     CDS             complement(64138..64860)
                     /locus_tag="OO339_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5925336.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO339_000593"
                     /pseudo
     CDS             complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO339_000593"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66023..66778)
                     /locus_tag="OO339_000594"
     CDS             complement(66023..66778)
                     /locus_tag="OO339_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5925337.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66860..67423)
                     /locus_tag="OO339_000595"
     CDS             complement(66860..67423)
                     /locus_tag="OO339_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5925338.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16871 a        18260 c        16753 g        15636 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaatgac
      601 gccttttatt gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgtttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaagaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagg ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata caaccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
    63061 cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt
    63121 tacaaacaag cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga
    63181 atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa
    63241 gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac
    63301 aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc
    63361 cggcctcagc ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg
    63421 aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc
    63481 atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct
    63541 gccggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg
    63601 ttattgacgg taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt
    63661 ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt
    63721 gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag
    63781 aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc
    63841 tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg
    63901 atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg
    63961 ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga
    64021 tctggcgtta cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc
    64081 gtagcaccca atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta
    64141 ttggttagct gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg
    64201 aatgacaggc tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg
    64261 cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg
    64321 cgcaacacca ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac
    64381 attgccgtca tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc
    64441 aaacggagga tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat
    64501 gacggcaaat ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg
    64561 ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata
    64621 gttattgacg tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt
    64681 accttctacc ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat
    64741 attctgggta cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga
    64801 caagcccgca gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag
    64861 cgaaccatca tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt
    64921 caactgatag tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt
    64981 cccactgcca tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc
    65041 gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag
    65101 atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt
    65161 gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc
    65221 gcatcgcgga agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt
    65281 accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag
    65341 aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc
    65401 acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg
    65461 gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca
    65521 ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg
    65581 ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc
    65641 cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat
    65701 ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga
    65761 atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga
    65821 acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt
    65881 gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca
    65941 gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc
    66001 tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc
    66061 cactcccatt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    66121 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg
    66181 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    66241 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    66301 ccctgtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    66361 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66421 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66481 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66541 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66601 caacatatcc ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca
    66661 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66721 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66781 acgtttctat ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt
    66841 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat
    66901 tacccagatc tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct
    66961 tcgctttgcc tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    67021 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    67081 cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    67141 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    67201 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    67261 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    67321 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg aacacgccca
    67381 tcaccattgc cgcagcaata gctgttttaa caaacttact cattatttgt ccttaaacta
    67441 taaataatta aaaatataaa cattgcaata cattgattca gtcaatagcc aatgtttata
    67501 cccagccatt gaatacggca
//
LOCUS       DAJTRH010000008        66645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTRH010000008 DAJTRH010000000
VERSION     DAJTRH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO339_000596"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO339_000596"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5925339.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO339_000597"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO339_000597"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5925340.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO339_000598"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO339_000598"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5925341.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO339_000599"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO339_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5925342.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO339_000600"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO339_000600"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5925343.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO339_000601"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO339_000601"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5925344.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="OO339_000602"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="OO339_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5925345.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO339_000603"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO339_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5925346.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO339_000604"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO339_000604"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5925347.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO339_000605"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO339_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5925348.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO339_000606"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO339_000606"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5925349.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="OO339_000607"
     CDS             complement(15666..16703)
                     /locus_tag="OO339_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5925350.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="OO339_000608"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="OO339_000608"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5925351.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OO339_000609"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OO339_000609"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5925352.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="OO339_000610"
     CDS             19445..19885
                     /locus_tag="OO339_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5925353.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OO339_000611"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OO339_000611"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5925354.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21602)
                     /locus_tag="OO339_000612"
                     /pseudo
     CDS             complement(20612..21602)
                     /locus_tag="OO339_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OO339_000613"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OO339_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5925355.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OO339_000614"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OO339_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5925356.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OO339_000615"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OO339_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5925357.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OO339_000616"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OO339_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5925358.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OO339_000617"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OO339_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5925359.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OO339_000618"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OO339_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5925360.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OO339_000619"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OO339_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5925361.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OO339_000620"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OO339_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5925362.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="OO339_000621"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="OO339_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5925363.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OO339_000622"
     CDS             complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OO339_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5925364.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OO339_000623"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OO339_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5925365.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OO339_000624"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OO339_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5925366.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OO339_000625"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OO339_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5925367.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OO339_000626"
     CDS             complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OO339_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5925368.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36827..37423
                     /gene="rsmD"
                     /locus_tag="OO339_000627"
     CDS             36827..37423
                     /gene="rsmD"
                     /locus_tag="OO339_000627"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5925369.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37413..37682
                     /locus_tag="OO339_000628"
     CDS             37413..37682
                     /locus_tag="OO339_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5925370.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37685..38044)
                     /locus_tag="OO339_000629"
     CDS             complement(37685..38044)
                     /locus_tag="OO339_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5925371.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38185..38811
                     /locus_tag="OO339_000630"
     CDS             38185..38811
                     /locus_tag="OO339_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5925372.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38885..41083
                     /gene="zntA"
                     /locus_tag="OO339_000631"
     CDS             38885..41083
                     /gene="zntA"
                     /locus_tag="OO339_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5925373.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="OO339_000632"
     CDS             complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="OO339_000632"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5925374.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41651..42316
                     /locus_tag="OO339_000633"
     CDS             41651..42316
                     /locus_tag="OO339_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5925375.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42389..42946
                     /gene="dcrB"
                     /locus_tag="OO339_000634"
     CDS             42389..42946
                     /gene="dcrB"
                     /locus_tag="OO339_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5925376.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42950..44167)
                     /locus_tag="OO339_000635"
     CDS             complement(42950..44167)
                     /locus_tag="OO339_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5925377.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44301..45350
                     /locus_tag="OO339_000636"
     CDS             44301..45350
                     /locus_tag="OO339_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5925378.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45405..45992
                     /gene="acpT"
                     /locus_tag="OO339_000637"
     CDS             45405..45992
                     /gene="acpT"
                     /locus_tag="OO339_000637"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5925379.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46103..47677
                     /gene="nikA"
                     /locus_tag="OO339_000638"
     CDS             46103..47677
                     /gene="nikA"
                     /locus_tag="OO339_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5925380.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47677..48621
                     /gene="nikB"
                     /locus_tag="OO339_000639"
     CDS             47677..48621
                     /gene="nikB"
                     /locus_tag="OO339_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5925381.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48618..49451
                     /gene="nikC"
                     /locus_tag="OO339_000640"
     CDS             48618..49451
                     /gene="nikC"
                     /locus_tag="OO339_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5925382.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49451..50215
                     /gene="nikD"
                     /locus_tag="OO339_000641"
     CDS             49451..50215
                     /gene="nikD"
                     /locus_tag="OO339_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5925383.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50212..51018
                     /gene="nikE"
                     /locus_tag="OO339_000642"
     CDS             50212..51018
                     /gene="nikE"
                     /locus_tag="OO339_000642"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5925384.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51024..51425
                     /gene="nikR"
                     /locus_tag="OO339_000643"
     CDS             51024..51425
                     /gene="nikR"
                     /locus_tag="OO339_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5925385.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51545..51904)
                     /locus_tag="OO339_000644"
     CDS             complement(51545..51904)
                     /locus_tag="OO339_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5925386.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51901..52176)
                     /locus_tag="OO339_000645"
     CDS             complement(51901..52176)
                     /locus_tag="OO339_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5925387.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52235..53359)
                     /locus_tag="OO339_000646"
     CDS             complement(52235..53359)
                     /locus_tag="OO339_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5925388.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="OO339_000647"
     CDS             complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="OO339_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5925389.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56091..57158)
                     /locus_tag="OO339_000648"
     CDS             complement(56091..57158)
                     /locus_tag="OO339_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5925390.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57524..59146)
                     /locus_tag="OO339_000649"
     CDS             complement(57524..59146)
                     /locus_tag="OO339_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5925391.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59408..60997)
                     /locus_tag="OO339_000650"
     CDS             complement(59408..60997)
                     /locus_tag="OO339_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5925392.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61382..62413
                     /locus_tag="OO339_000651"
                     /pseudo
     CDS             61382..62413
                     /locus_tag="OO339_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62500..62976)
                     /locus_tag="OO339_000652"
     CDS             complement(62500..62976)
                     /locus_tag="OO339_000652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925393.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62969..63733)
                     /locus_tag="OO339_000653"
     CDS             complement(62969..63733)
                     /locus_tag="OO339_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5925394.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63979..64740
                     /locus_tag="OO339_000654"
                     /pseudo
     CDS             63979..64740
                     /locus_tag="OO339_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64847..65347)
                     /locus_tag="OO339_000655"
     CDS             complement(64847..65347)
                     /locus_tag="OO339_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925395.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65498..>66645)
                     /locus_tag="OO339_000656"
     CDS             complement(65498..>66645)
                     /locus_tag="OO339_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5925396.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16192 a        17890 c        16346 g        16217 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcac atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctctag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagctgcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcagcgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctacttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg ccccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaaca gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcagcac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag cgtgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg gaagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggccagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaaaatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcgg gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gcccttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgcgc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agcttgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcagattaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaacagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttctgccgc ttcgacaatc tctacggttt ccgcttcagc ctgccactct
    36301 tctgacgaaa cctcttcggc gttgacgtct tccggcaacg gcagctcttc acgttcgata
    36361 gccactggct ccggcgtttc ttctacgacc ggttccggct gtgcaacaac ttccgcttca
    36421 ggctgcgctt tttcactttc aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa
    36481 gtttccgcct cggcttcagt atgcgcctgc ggctgctctt caacggcttg ttcagaagcc
    36541 ttcgcaggct cttgcgcctg aacgatttct tctacaaccg gttgttcatt ctgaacttct
    36601 gtctcttttt ccggggtctg ctctttttga ccaaagccca gccaggaaaa aaagccacgt
    36661 cttttttctt tcgccatttg cgactacact cctcgctgtt gcttcatggc acagcgttaa
    36721 cgctatgtac atagcagcta aaaaaatgat gaaatagtct atcacttaac ttaattcaca
    36781 tcaccgcctg caagtatgtg ttatctggcg gattgagcaa tttatcatga aaaaaccgaa
    36841 tcattccggc agcggccaaa tccgcattat tggcgggcag tggcgaggcc gtaaactccc
    36901 ggttcctgat agcccaggtc tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa
    36961 ctggctggct ccggtcattg ttgacgccca atgtctggat tgcttcgccg ggagcggcgc
    37021 gctggggctg gaagcgttat cgcgctacgc tgcgggggca acgttgattg agatggatcg
    37081 cgtggtttct cagcagttaa ttaagaatct ggcgacacta aaagcaggca atgcacgcgt
    37141 ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc acaccgcata atatcgtgtt
    37201 tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg ataaatttac tggaagataa
    37261 cggctggctg gctgacgaag ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc
    37321 cactgttccg gcaaactggt cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg
    37381 gctgtatcaa cacgaagcac aaggagaaag tgatgctgat taatattggt cgtttgttaa
    37441 tgctctgcgt ttggggattt ttaatcctca atctggtgca tcccttcccg cgcccgctaa
    37501 atattttcgt taacgtggcg ctgattttta ccgtgctgat gcatggtatg cagctggcgc
    37561 tattgaaatc cactttaccg aaagatggcc cgcagatgac caccgccgaa aaggtgcgga
    37621 ttttcctttt cggcgtgttt gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat
    37681 aaccttattg ttcgccaaca aagctaacaa agcgcgttcc tttatagctc agcgtacctt
    37741 tatcgcccac tgtcagggcg tggtactgct gggcgtcgag gcgaaacgtc tgctccattc
    37801 ctccgctttg cggtttgaag cttgcctcat agcgcatact ggtgcctgcc ggagtcactt
    37861 cctgctggcg cgaacggcga tcgttaatcg gtttttcccg cttgttgctc accaccacca
    37921 gcttttgctg gagcggagcc atatcattat cagctttttc ccgtcgctgt tgcataaaac
    37981 gaaacgatgc ggcgacgaca attaagccaa tgataacaat aaagaaaaga ggtggtttac
    38041 tcatctttat ccctcatcgg aaaatgcgga attaagcata ccctgccagt tatggtgttg
    38101 tcatccgtcc accctcgcca ctaaactgga agcaagaccg taggcattct gcttacgaaa
    38161 aaataacgaa ttcaaggaac taagatgctt tggtcgttta tcgctgtctg tctttccgca
    38221 tggctatctg tggatgcatc gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg
    38281 ttaacccttc ttctcctgct gttactggcc tggcaagcac cgatgttcga cgccattagc
    38341 tatctggtgc tggcgggact gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca
    38401 cgtcaacgtc tgatgtacgc catcggcgcg tttttcctct cgcacctgct gtacaccatc
    38461 tatttcgcca gtcagatgac gctctctttc ttctggcctc tgccactggt gttgctggtt
    38521 ctgggtgcgc tgctactggc gattatctgg acgcggctgg aagagtaccg ttggcctatc
    38581 tgcacgttta tcggcatgac gctggtgatg gtgtggctgg caggtgaact gtggttcttc
    38641 cgtccgaccg ctccggcgct ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac
    38701 tttgtctggc tggggagcca ctatcgccga cgcttccgtg cggataacgc gattgctgcg
    38761 gcctgctact ttgccggtca cttcctcatc gtccgctcac tgtatctctg ataaaacttg
    38821 actctggagt cgactccaga gtgtatcctt cggttaatga gaaaaaactt aaccggagga
    38881 tgccatgtcg acacctgaca atcacggcaa gaaagcccct caatttgctg cgttcaaacc
    38941 gctaaccacg gtacagaacg ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc
    39001 aacgctctct gaaaacgtct ccggcacccg ctatagctgg aaagtcagcg gcatggactg
    39061 cgccgcctgt gcgcgcaagg tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt
    39121 gcaggtgttg ttcgccaccg aaaaactggt ggttgatgcc gacaatgaca tccgtgccca
    39181 ggttgaatct gcggtgcaaa aagcgggcta ttccctgcgc gatgagcagg ccgccgacga
    39241 gccacaagca tcgcgcctga aagagaatct gccgctgatt acgcttatcg tgatgatggc
    39301 aatcagctgg ggtctggagc agttcaatca tccgttcggg caactggcgt ttatcgcgac
    39361 cacgctagtt gggctgtacc cgattgctcg tcaggcatta cggctgatca aatccggcag
    39421 ctacttcgcc attgaaacct taatgagcgt agccgctatt ggtgcactgt ttattggcgc
    39481 aacggctgaa gctgcgatgg tgttgctgct gtttttgatt ggtgaacgac tggaaggctg
    39541 ggccgccagc cgcgcgcgtc agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc
    39601 cacgcgcctg cgtaacggtg agcgggaaga ggtggcgatt aacagcctgc ggcctggcga
    39661 tgtgattgaa gtcgccgcag gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt
    39721 tgccagtttt gatgaaagcg ccctaaccgg agaatccatt ccggtggagc gcgcgacggg
    39781 cgataaagtt cctgcaggag ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc
    39841 agaaccgggt gccagcgcca ttgaccggat tctgaaactg atcgaagaag ccgaagagcg
    39901 ccgcgccccc attgagcggt ttatcgaccg tttcagccgt atttatacgc cagcgattat
    39961 ggccgtcgcc ctgctggtaa cgctagtgcc gccgctgctg tttgccgcca gctggcagga
    40021 gtggatttat aaaggtctga cgctgctgct gattggttgc ccgtgtgcgt tagttatctc
    40081 aacgcctgcg gcgataacct ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa
    40141 aggcggtgcg gcgctggaac aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg
    40201 tacgctgacc gtcggtaaac cgcgcgttac cgcgattcat ccggcaacgg gtattagtga
    40261 atctgaactg ctgacactgg cggcggcggt cgagcaaggc gcgacgcatc cactggcgca
    40321 agccatcgta cgcgaagcac aggttgctga actcgccatt cccaccgccg aatcacagcg
    40381 ggcactggtc gggtctggca ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc
    40441 tgccgggaaa catcccgctg atgcatttgc tggtttgatt aatgaactgg aaagcgccgg
    40501 gcaaacggtt gtgctggtag tacgtaatga tgacgtgctg ggtatcattg cattgcagga
    40561 taccctgcgc gccgacgcta caactgccat cagtgaactg aacgcgctgg gggttaaagg
    40621 agtgatcctc accggcgata atccacgcgc agcggcggca attgccgggg agctggggct
    40681 ggagtttaaa gcgggcctat tgccggaaga taaagtcaaa gcggtgacca agctgaatca
    40741 acatgcgccc ctggcgatgg tcggtgatgg tattaacgac gcgccagcga tgaaagctgc
    40801 cgccatcggg attgctatgg gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc
    40861 attaacccat aaccacctgc gcggcctggt gcaaatgatt gaactggcac gcgccactca
    40921 cgccaatatc cgccagaaca tcaccattgc gctgggactg aaagggatct tcctcgtcac
    40981 cacgctttta gggatgaccg ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt
    41041 gctggtgacg gcgaatgcgt taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac
    41101 attgagcgcg atcggtcccc tcgcccctct ggggagaggg ttagggggtg aggaaaaggc
    41161 ggcatcgaag ccaatcagcc cctatcaacc gcctttacga atcaaataac gataaggcag
    41221 tccatccgtc tctttcgcaa ccagttcgtg ttccataaag gtacaaaacc caggaatatc
    41281 gcgggtagtg gccggatcgt cggcgataat cagcaacgtt tcgccaggct gcatattgcg
    41341 cacggttttg cgcaccatca tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt
    41401 gtggtcaggg ctggaaaaga gatcggtcat tttcttctca tcacttaaaa aaacggcgct
    41461 agtttacgcc ctgtgagtcc gtaagcaaac caggttaacg attgcgtgaa aattaaccat
    41521 tgcattgtca acgtaaagca gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt
    41581 atgttaaggt aacagacgtg tcgtacgtat tgggttccct caccccaatg gttaatcaaa
    41641 aaggtacaat atgaacgttt tctcgcaaac tcaacgctat aaggcgttgt tctggttatc
    41701 gttatttcat cttctggtga tcacctccag taactatctg gttcagcttc ccgtctccat
    41761 ttttggtttc cataccacct ggggcgcgtt tagctttccg tttatttttc ttgctaccga
    41821 cctgaccgtg cgtatttttg gcgcaccgct ggcccgacgc attatcttcg cggtaatgat
    41881 ccctgcgtta ttaatctcct acgtcatctc gtcgctattc tatatgggtt cctggcaggg
    41941 attcggcgca ctcgcccact tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat
    42001 ggcctacgcg ctggggcaaa tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg
    42061 ccgctggtgg ctggcaccga cagcgtccac actgttcggt aacgtcagcg acacgctggc
    42121 ctttttcttc attgccttct ggcgtagccc ggatgccttt atggctgaac actggatgga
    42181 aatcgcgctg gtcgattact gtttcaaagt gttaatcagt atcgttttct tcctgccaat
    42241 gtatggcgta ttactcaata tgctgttgaa aagactggca gataaatccg aaatcaacgc
    42301 tttgcaggcg agttaaaggt tcgttatcag agttgtgata agatggatga atgagccgtt
    42361 atggccgttt atcgaaagga agaagtcaat gcgcaatctg gttaaatatg tcggaattgg
    42421 cctgctggtt atggggcttg cggcctgtga tgataaagac actaacgcta cggcgcaggg
    42481 ttcggttgcg gaaagtaacg ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag
    42541 tttctcgctg ccagcggata tgaccgacca gagcggtaag ctgggaacgc aggccaataa
    42601 catgcatgtc tggtccgacg ccaccgggca gaaagcagtc atcgtcatca tgggcgatga
    42661 tccgaaagaa gatctggcgg tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga
    42721 tccgcagctg caagtggtaa ccaataaagc cattgagctg aaaggtcaca aaatgcagca
    42781 gttagacagt attatctccg cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa
    42841 cgtgggtaat caactgctga ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc
    42901 gcaaaccacc gcagaaaaca tcattaatac gctggttatt cagtaagttt taagatgatg
    42961 aggcggcctc agggacgtgt tccggaggcc gtttttttaa tcgccacgtc agtaataacg
    43021 cgattgcgac cagtcccgcc gccgccagat aaatcaccgg tacgcccgcc cagctcatca
    43081 ccagcccagc cagtggtcca gtcacgccaa gcgataaatc cataaatacg gtgtaagttg
    43141 ccagcgccgc cccctgattt tgctgcggaa ccgcttttac cgccactaca cccaatgccg
    43201 ggaacaccag cgaaaacccg gcccccgcca gtaagacgcc gattttcgcc atccacggca
    43261 tagtcgccac gccaaccagt agcaggccga ttatctcaac gctaaagcaa atcatcgcta
    43321 cgtttaagcc accgatacgg ttaatgccgt tagggaataa caaacgcgta ccgacaaacg
    43381 cacagctaaa cagcgtcagc gcgaaagccg caccgtccca acctttagcg tcataaaaca
    43441 gcgtgataaa ggtggcgatg acgccaaatc cggcggaagc cagtgccagc gccataccgt
    43501 acagccagac gcgcccaagc accgcgcgaa acggcagcgg tttgccttta ctggctttta
    43561 ccgtcggacg cgggatcgcc aacaaaatgg ccaccagcgc cacgcccata atgattaacg
    43621 ctaacgcctg caagccgccc cagtgataaa acacgacgcc taacggcgca cccatcgcca
    43681 tcgccccgta agtgacaatg ccgttccacg aaatcacccg cccgatatgc agcgagccaa
    43741 ccactccaac gccccatagg gtcgatcccg ttccggcaaa actttgccca atcccaagga
    43801 tgacgcgccc caggcaaagt aataacaggc tgatgacagg cagactggcg gttaatcctg
    43861 ccgtcagata ccccagaccg ctcaaaaagc agccgcataa accgaagacg acaatctttt
    43921 tgggtcccag caaatcggcg taacgtccgg catgagggcg gctcagcaag gtggcgaaat
    43981 attgcaggct gataaccaat cctgcccaga aggcgctaaa gcccatcaca tcatggacat
    44041 agcccggtaa tacagcgagc ggcaacccga tggtgaggta gctggcgaag ttaaacatga
    44101 caatggagac aatgcgcaaa ttcaggcgca atccgtttag cgcgggttcg gctacgggtt
    44161 cgggcatgag gatcaccaca tttttacaac agtgtttcat ttttaccatg tgctgacgtg
    44221 aaaatcagca gtaagaatca gaatattgct ggcgtgactc ccgcgctaca cttaacacaa
    44281 aaagtcacaa ggaagcccca atggaaaccc ctcaacccga taaaacgggc atgcacattc
    44341 tgctcaagtt ggcctcgctg gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca
    44401 ttgtgcagct gttactggcg ctgttttttg ccatcgtcct caacccgctc gttacctggt
    44461 ttattcgtcg gggagtacaa cgccccgttg ccattacgat tgtagtggtg gtgatgctga
    44521 tcgcactaac cgcgctggtc ggcgtactgg cggcatcgtt taacgaattt atctctatgc
    44581 tgccgaagtt taataaggag ctgacgcgca aactttttaa attgcaggag atgttgcctt
    44641 ttcttaattt gcatatgtcg ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg
    44701 ttaccttcac cacagcgcta atgaccgggc tttccggggc aatggcgagc gtgcttttgc
    44761 tggtgatgac cgtagttttt atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt
    44821 ttgcgctgaa taacccacag attcacatcg caggattaca ccgcgcactt aaaggcgttt
    44881 cgcattatct ggcgttaaag acgctactca gtttatggac aggtgtcatc gtctggctgg
    44941 ggctggcgct aatgggcgta cagtttgcgc tgatgtgggc agtactggcg tttttgctca
    45001 actacgtgcc caatatcggc gcggtaattt ccgccgtacc gccaatgatt caggtgctgc
    45061 tgtttaatgg tgtttacgaa tgtattctgg tcggcgcatt gtttttagtg gtccatatgg
    45121 tcatcggcaa tattttagaa ccacggatga tgggccatcg cctggggatg tccaccatgg
    45181 tggtatttct ttcattgtta atctggggat ggctgctcgg cccggtaggg atgctacttt
    45241 cggtgccatt aaccagcgtg tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac
    45301 tggcgatttt actggggccg ggcagaccga aaagtcggtt accgggatga ggcgacaagt
    45361 gatacgatac gcactttcat tttccattaa acgttggccc tgatatgtat cggatagttc
    45421 tggggaaagt ttcgacctta agcgcagctc cactgccacc gggtttacgc gagcaagcac
    45481 cgcaaggtcc acgacgcgaa cgctggctgg cggggcgtac attgctttcg cacacgcttt
    45541 ccccgctacc ggagatcatc tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc
    45601 cgctatggtt caacttaagc catagcggtg acgatatcgc tctgctgttg agcgatgaag
    45661 gcgaagtcgg ctgcgatatc gaagtgattc gcccgcgcgt caactggcgc tggctggcga
    45721 acgccgtgtt cagcctcggg gaacacgctg aaatggacgc cgtgcatcct gatcagcaac
    45781 tggaaatgtt ctggcgcatc tggacgcgca aagaagccat cgttaaacag cgtggcggca
    45841 gcgcctggca aatcgtcagc gtagatagca cctatcactc ctcgctgtca gtcagccatt
    45901 gtcagctaga aaatttaagc cttgcgatct gcacccccac cccctttacg ctcaccgccg
    45961 acagtgtgca atggatcgat tcagttaact gatccgccca cccgactgcc catctattga
    46021 tccagaacag gtaatcagta tgacgaatac ttaaaatcgt catacttatt tccgccatct
    46081 attttaatcc attggggtta ccatgctctc cacactccgc cgcactctat ttgcgctgct
    46141 ggcttgtgcg tcttttatcg tccatgccgc tgcaccagat gaaatcacca ccgcctggcc
    46201 ggtgaatgtc gggccactaa acccgcacct ttacacgcct aaccagatgt tcgcccagag
    46261 catggtttat gaaccattgg tgaaatatca ggcagacggt tcggtgatcc cgtggctggc
    46321 aaaaagctgg actcattcag aagatggtaa aacctggacc ttcaccctgc gtgatgacgt
    46381 gaaattctcc aacggtgaac cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt
    46441 gctcgataac cgtcaacgtc acgcctggct ggagctggca aaccagattg ttgatgttaa
    46501 agcactcaat aaaacagagc tgcaaattac cctgaaaagc gcctactatc ctttcctgca
    46561 agaactggcc ctgccccgtc ctttccgttt tatcgctccc tcgcagttta aaaaccatga
    46621 aaccatgaac ggaattaaag cgccgattgg caccggaccg tggattttgc aggaatcgaa
    46681 actaaatcag tacgatgtct tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat
    46741 taaaaagatc acctttaacg tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac
    46801 tggcgatatc gacctgctgt acggaaacga agggttatta ccgctcgata ccttcgcccg
    46861 ctttagccag aatccggctt accacaccca actgtcacag ccgatcgaaa ccgtgatgct
    46921 ggcgctcaat accgccaaag cccccaccaa cgagctggca gtacgtgaag ctcttaatta
    46981 cgcggtaaac aaaaaatcgc tgattgataa cgcgttatat ggcacccagc aggtcgccga
    47041 caccctgttt gccccttctg tgccttacgc taatctcggc ctgaaaccgc gccagtacga
    47101 tccgcagaaa gcgaaagagt tgctggaaaa agccggttgg acgctgcctg cgggcaaaga
    47161 catccgcgag aaaaatggtc agccgctgcg cattgaactg tcgttcatcg gcaccgatgc
    47221 gttaagcaaa tcgatggcgg aaatcattca ggctgatatg cgccagattg gcgcagatgt
    47281 ctcgctgatt ggcgaagaag agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg
    47341 catgattttc caccgcacct ggggcgcgcc atacgatcca cacgccttcc tgagttcaat
    47401 gcgcgtaccg tcgcacgctg acttccaggc acagcaagga ttagccgaca agccgctgat
    47461 tgataaagag atcggcgaag tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta
    47521 tcgcgacatt ctgacccgtc tgcatgacga ggcggtttat ctgcctatca gctacatctc
    47581 gatgatggtg gtatcaaaac cggagctggg taatatcccc tacgcgccga tcgccaccga
    47641 aattccgttc gaacagatta aaccggtgaa accttaatgt tgcgttacgt attacgccgc
    47701 tttctgctgc tgatcccgat ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc
    47761 ctcggtaccg gcgacccggc gctcgattat ctgcgtttat ctaacctgcc gccgacgccg
    47821 gagatgctgg cctctacccg caccatgctc gggctggatc agccgctgta cgtccagtac
    47881 ggtacctggt tgtggaaggc gctgcatctt gactttggta tctcattcgc cagccaacgc
    47941 ccggtactgg acgatatgct gaacttcctg cccgccacgc tggaacttgc aggtgcggcg
    48001 ctggtattaa ttctgctcac ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat
    48061 cgtttgccgg atttcgccgt gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc
    48121 tggctggcat ttttgctagt gatggcgttt tcggtgtatc tgcaatggct acccgcgatg
    48181 ggctacggca gctggcagca catcatttta cctgcggttt ccatcgcctt tatgtcgctg
    48241 gcgattaacg cgcgactgct gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc
    48301 acctgggcgc ggctacgcgg cctgaatgac aaacagaccg aacgccgcca tattctgcgc
    48361 aatgcctcgc tgccgatgat caccgccgtg gggatgcaca ttggcgaatt gattggcggg
    48421 acgatgatta tcgaaaacat ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg
    48481 atttttaacc gtgactatcc ggtgattcag tgctttacgc tgatgatggt ggtggttttt
    48541 gtggtctgta atttgattgt cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt
    48601 catgaaggag cgcacgcgtg aactttttcc tctcttcccg ctggtcggta cgcctggcgc
    48661 tgatcattat cgccctgctg gcgctgattg cgctcaccag ccagtggtgg cagccgtacg
    48721 atccacaggc aattgatttg ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg
    48781 gcaccgatca cttaggtcgc gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc
    48841 tcggttcggt aatggcctgc ctgctgctgg tgctgacatt agggctggtt attggcggca
    48901 gcgccgggtt gattggcggg cacgttgatc aggccaccat gcgcgtcgcc gatatgttta
    48961 tgaccttccc gacctcgatt ctgtcgttct ttatggttgg cgtgctcggc accgggctga
    49021 ccaacgtaat tatcgccatc gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca
    49081 gcttggtgat ttcactacgc caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg
    49141 gccatgtgcg ggtgtttgtc gatcatctgg caggcgcggt gatcccttcg ctgctggtgc
    49201 tggcaacgct ggatatcggc catatgatgc tgcacgtcgc ggggatgtct ttccttggcc
    49261 tcggtgtgac cgcgccgacc gccgaatggg gcgtgatgat taacgacgcg cgccagtata
    49321 tctggaccca gccgctgcaa atgttctggc cggggctggc gctgtttatc agcgtgatgg
    49381 cctttaacct ggtgggtgac gcactgcgcg atcatctgga tcctcatctg gtgacggagc
    49441 acgcacacta atgccgcaac agattgaact acgtaatatc acgctacagg ccgcgcagcc
    49501 gctggtacac ggtgtatcgt taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg
    49561 tagcggcagc gggaaatcat taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt
    49621 tcgccagacg gcgggggaaa ttttagccga tggcaaaccg gtttcgcctt acgccctgcg
    49681 cggcatcaaa attgccacca tcatgcagaa cccgcgcagc gcctttaatc cactgcacac
    49741 catgcacacc cacgcgcggg aaacctgcct ggcgttaggg aaacccgccg atgacgctac
    49801 gcttaccgct gccatagaag cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta
    49861 cccgttcgag atgagcggcg gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg
    49921 tgaatcaccg tttatcatcg ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc
    49981 gcgcatcctc gatctgctgg aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt
    50041 cacccatgat atgggcgttg tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg
    50101 taaaattgtc gaacagggcg atgtagaaac gctgtttaac gcccccaaac atacggtgac
    50161 gcgcagcctg gtttccgctc atctcgccct ctacggtatg gagctggcat catgacctta
    50221 cttaacgtct gcggcctttc ccatcactat gcccacggtg gatttagcgg aaaacatcaa
    50281 catcaggcgg tgctgaataa cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg
    50341 ctgggacgca gcggctgcgg aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct
    50401 ccctcacaag ggaatattag ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa
    50461 cgtaaagcct tccgccgtga tattcagatg gtgtttcagg actccatcag tgccgtgaat
    50521 ccgcgcaaaa ccgtgcgcga gatcctgcgt gaaccgatgc gccacctgct atcactgaaa
    50581 aaatccgaac aactggcgcg cgccagcgaa atgctgaagg cggtcgatct cgatgacagc
    50641 gttctcgaca aacgcccacc gcagttaagc ggcggccagc tccagctcgt ctgcctggca
    50701 cgcgcgctgg cggtcgaacc gaaattgctg attctggatg aagccgtttc taaccttgat
    50761 ctcgtgttac aggcgggcgt cattcgcctg ctgaaaaagc tacaacaaca gtttggcact
    50821 gcctgcctgt tcatcactca tgacttacgc ctggtggaac gcttttgcca gcgagtgatg
    50881 gtgatggaca acggacaaat cgccgaaacc caggcggtgg gagataaatt aaccttttcc
    50941 tctgacgccg gacgtgtgct acaaaacgcg gtattacccg cattccccgt gcgccgtcgc
    51001 acctcagaaa aggtttaacg ccaatgcaac gagtcaccat cacgcttgat gacgatttac
    51061 tggagacgct ggacagcctg agccagcgtc gtggttataa caaccgttcc gaagctatcc
    51121 gcgatattct gcgtagtgcc ctggcgcagg aagctaccca gcagcacggc acacaaggtt
    51181 tcgcggtgct gtcgtatgtg tatgaacacg aaaaacgcga cttagccagc cgtattgttt
    51241 ccacccagca tcatcaccac gacctctccg tcgccacgct acatgtgcac atcaaccacg
    51301 acgactgtct ggaaatcgcc gtgttgaaag gtgacatggg tgacgtgcag cattttgccg
    51361 atgacgttat cgcccagcgc ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag
    51421 attgagttat ttctaaaaca aggaaagaaa agatattaac tgaacttaat ttataaaatt
    51481 aagttcagtt gcatagatag atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta
    51541 aaatttataa atgattcaaa cgctcattca gtgtttcaat aatataggta ttaacagaga
    51601 cttgttgctg cgcagccgca ttattaaggc gttcactcag cgattctgga tagcgtaacg
    51661 taaaggtctt aatcttttct gtacgcgcat acggctcaat acccgcagct ttacaatcct
    51721 caagatactc tcgcagggaa agctctcctt ctttttgcag cccctgaata ctgtcagaga
    51781 caaaatcaca ataacctgac aagcccagga atttgccacg aaatgcattc agttctggca
    51841 cgtaggtaat aacagctggt tgcccggcaa tctccataga gtttggcgtc ttcagtttga
    51901 tcatggtttt acccctatac ttagtaacca gtcacacacg ctttctacag cgcctttatc
    51961 ggtatctggc gacggatgag gccgatggaa acacgcaacg ctcatattta gtatgaactt
    52021 gcaacgcgaa cctcttcctt ccttaatttc tccgcctaat gctttaacca gtgactctat
    52081 atcagaccac ttgatccctt gaggaacagg agttttaaat atttgatcca atgtattttt
    52141 ttgtttcttc cgtaaagcct gtaccttggt tctcatggca tgtccttatg acttcacttt
    52201 atgacatcac agtatgaagt caagtttccc ttccttacgc cattgtccca atcgtcttcc
    52261 tgaatcgcag cagtgcgatg gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact
    52321 gcggccagac gatttcgaat ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa
    52381 agtgtgtcgt cggcatggtc agcataatgt cctgcaccat ctgcggcata ctttcgcgcg
    52441 gcgtggatcc accggaaagc atttgcagcg gcagcagcac cagaatcacc agcagcccca
    52501 gttgcggcat tgaacgcgct atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca
    52561 gactgagcgc cacgcccagc ataaacagcg ggatcgagcc ttcaatcggt acgcccagta
    52621 cacctttcac catcagcacc agcgataatc ccgataccac cagcaccacc agccccatcg
    52681 accagatctt cgccatcatg atctcaaacg gcgttatcgg catcaccagt aaatgttcca
    52741 ccgtgccgtg ttcacgctcg cggatcagcg ccgatccggt caatacaatc gccagcatgg
    52801 taatgttgtt gatgatcgcc atcaccccgc caaaccacgc gggatcaagg ttcgggttaa
    52861 agcgcatccg ggtttccagc gataccaacg gttcgctgtt atcacggtag cgcgcgacaa
    52921 agctgttcac ttcaccgttg ataatattct ggatataccc attgccggta aatgcctggc
    52981 tcatgcgcgt ggcatcgacg ttcacctgaa tatccggctg gcgtccggcg aggacatcac
    53041 gctgaaaatt aggcggaata tttatcgcga aggtatagcg tccggcgtcc agtccggcat
    53101 ccatctcatc ggcggtgatc atctccggtg gcaaaaacca cggacgatag aagctgttaa
    53161 cgatccggtt cgataactgc gattgatcca tatcggcaat ggcgatcggc gcgaggttca
    53221 acgatcctgg cgtaacggtc gctgacgaat acaccgacac cgtaaacgag aagacaatca
    53281 gcgtcagcat cgctttatca ccgagcagac tgcgcaactc tttgataccc agattaaaaa
    53341 tattgcgtaa atggcgcatc atccctcctg ttttttcagc agcaggatgc ttaagcccat
    53401 caccagcggg atggctatca gcaacgggat aaaaagttgc cacaaatcag tcagatccag
    53461 cgctttcgag aacgttccgc gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc
    53521 gatccaacgt ccaggccctt ccagcgaagc taccggatcg atcatcccgg aaaactgtgt
    53581 cgccgggatc aatgtgataa tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa
    53641 ggtggagatc agcagcccca ttccagtggc aatgatgata tacagcagcg ccgccaaggt
    53701 gagcgtcagg aaactgcctt tatgcggtac gccaaacaca aacaccgaca ggccgcagag
    53761 caggaaaaag ttcagcatcc ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc
    53821 gctacgggtg gtgggggtca cgtaaaggtt gataatcgac cctagctctt tttcccgcac
    53881 cacgctaagg gcgcttagca ttgacgggat catcatcagc agaagcggga tcaccgccgg
    53941 aacaatcgct ggcaggcttt ttacgtccgg gttatagcga taacgcgtct caatgttcat
    54001 cagcccgctt tggctggcgg gtgtcgattg tcggctcgcc acatcctgaa gccaactctg
    54061 gtgcattgcc tgcacgtaac cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat
    54121 ccagacgccg agttccacag gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat
    54181 ctcaatggcc accgtgatat cgcccgcacg catccgacga tcaagctcgt cataactggt
    54241 gagcggcggc tgttcgataa agtaacggga accggagagg ttgagtgtcc acgcctggct
    54301 actgacggtc tggtcgcggt cgagcaccgc aaagcgcagg ttttccacat ccatactgat
    54361 gccgtaaccc attatcagca tcaggatcac cgttcccatc agcgccagcg tcgaacgtac
    54421 tggatcgcgt cgcagttcca gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct
    54481 aaatccctga cgcggcgcgt gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt
    54541 gctctgccct gccgcttcct gcaaataggc gataaatgcc tcttccagac tggcggctcc
    54601 gcgtttctca accagttcct gcggtgtacc gctggcaagc acttttccgg cgtgcatcag
    54661 tgagatgcgg tcgcaacgtt ccgcttcgtt cataaagtgg gtggagatga agatagtcac
    54721 tttgtcctgg cgcgagagat cgaccatcaa ctgccagaac atatccctcg ccaccggatc
    54781 gacaccagaa gtaggctcat cgaggattaa catctccggg cgatgaatca ccgccaccgc
    54841 cagcgaaagc cgctggcgaa tgccgagcgg caatgactcc ggcagaacat cttcaacgtc
    54901 gttgagctta aaacgctcgc tcatttcagc cactcgcgcg ggaatttccg cttccgggat
    54961 gtgaaacaaa cgggcatgta actccaggtt ttgccgcacg gtgagttcgt tatagagcga
    55021 aaacgcctgc gacatatagc ccacacgacg gcgggtatcg atatcttttg gatcaaccgg
    55081 ttgcccaaat agccacgcct caccttcgct ggcgggcagc agtccggtaa gcattttcat
    55141 ggtggtggat ttaccgcagc cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat
    55201 gcggaaattg acgtgatcaa cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc
    55261 ttcgatggca atctctgcgt tttcaggttg atacggtggg atcactaccg cctgatgcgc
    55321 ctggcgttgc gcttgcggta acagattaat aaatgcttct tccagcgtgg cgctttgcgt
    55381 ttgctgccgc agctcttcgg cgctgccggt tgccagcact tctccggcat tcatcgctac
    55441 cagccagtcg aagcgttcgg cctcttccat ataggcggtg gcgaccagca cgctcatatt
    55501 gctctgccgc tggcgaatac tgtcgatcag atcccagaac tgggcgcggg agagcgggtc
    55561 aacccccgtt gttggctcat caaggatcaa cagttccggg tcgtggatta acgcgcagca
    55621 cagcccaagt ttttgcttca tcccgccgga gagtttacct gccggacgat cgcgaaacgg
    55681 tgctaacccg gtgctggtca gcagctcatt aattcgcgct tcccgctccg ctttatcgtg
    55741 accgaacagg cgagcgaaaa aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt
    55801 tttgcccagc ccctgcggca tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg
    55861 gtcgtgcata tcgccaccca gcaccaccac attgccctgc tcaatgacgc gggcaccgga
    55921 aatcagtgac aataaactcg acttcccgac gccatccgga ccaatcagcc cgaccatgca
    55981 acgggcctga atatcgagag tgatattgtt cagcgcaacg gtttttccat aatgctggct
    56041 cacgcccgcc agttgcgcga caggcgggac gggaaccagt tccagatgcg tcattgcggc
    56101 aacctcacca cgaggtcgtc aggccacgga agttcttcat tcacccgcac ccacgctacg
    56161 cccggtaaac cggttttgac atattccaga tgctgctgga gtaattccgg tgggatacgc
    56221 gctttgacgc ggaacatcag tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg
    56281 aactgggcga cactggcgac aaaactgatg gttgcaggaa tacgcagatc tggcgcggca
    56341 tcgaggatca gccgggcttc accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg
    56401 aagaaggtca tatagacgtc gctgagatcg accatattca gcacccgacc gcctgccgcc
    56461 agcacttccc ctggctcggc aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt
    56521 tcactgtcat cgatatctgc ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga
    56581 gtttgcgcct gaatgatatt ggtgcgtgct gcttctatag ccgctttaga agccgaaacc
    56641 tgggctttcg ctgattccag cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc
    56701 agctgttgcg cagaaatagc ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg
    56761 gcaacagagt ccagttcggc ctggcgttga ttgaccagcg actgtgcggc acgagtttca
    56821 ctttgtcgtt gctccagcaa agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt
    56881 tgcgcgatgg cttccagtcg ctgttcctgc aacacgcgag tatccatctt cgccagcact
    56941 tcgccttcgc gaacaaactg gccttctttc accagaatgg tgtcgatacg cccggcaatt
    57001 ttgctggcga tatccacttc cgtcgcttca atgcgcccat tgctgacagc aaagccttcc
    57061 ggcacacccg ccgggcgcaa cagccaccac gccacgacgg cagccaccgc cagtgccccg
    57121 acaacccacc acgccagatg gcgcttactc ttatccataa tcgacccgcc ataatccctg
    57181 taagcaaacg acgcgtgtta caactgccgc cgaacgacgt tcagcggatt cttccatccg
    57241 ggataagaag gaaacgacaa atacgaccag cgcgcagagc attgagcgcc aacggggaag
    57301 gcttagtgaa aacgggtagt aatgttgtca tcatcagcat tcctggccgt aaatgaaaca
    57361 cgcatactca taagtgtgat ctataaaact ggctgacaaa acggtatcca tcctgttgga
    57421 tcgcatttca cgcctgttca gttgcaggaa cagagcattc ctttttacgt tttatcaaca
    57481 gtctcaacca gatagtaaca ttaatattca tgttgtctat ggttcagagc tgtaacattg
    57541 tgcgccttct aatcaaaggc ataagtccat ttctgttttt ttcatgctgg taagacttcc
    57601 agggagtggt tatctctggc cctgcaccag ggttaatttg gcaattatgc atgctgaaca
    57661 ttcttccagc atgtttactg tcacgggcgt taagacctaa aactgtcccc gtttcaccta
    57721 tgcgttctct cccaccattt ccgttatgtg caacaacttg atttatacct aacatttgtg
    57781 aacatgctaa tagtgcttca agtttaggtc tgtaatctgt cgaaacagaa aaataacgat
    57841 taaaggatcg acaagagata gcccggctag ttttaaactt tttgccatta attatagcat
    57901 gatgcttttt attaataatt tccgctaatt cgaccggatc gagagcattg gtcatttcat
    57961 caatagagac agttatcggt cctataatgt aacgttttcg ctgatcatca aaagttatcc
    58021 catgatgatt tgccattatt ttgtatttgg ggtcatagaa acatacagga taatctttta
    58081 tcccgtcgta ggtatcctca tcaagtggtt tcatgcgcgt gaagttttgc atatagttac
    58141 cattgcaatt cgtttcatga tttccagcta atacatgaac attttcattg atactcatta
    58201 tgcgttttaa caaatcgatg ataaattgat cgccacagat attactaaat cgatcgccgg
    58261 tttggtcgcc aagaaacgta catggcgtga ctgcgtcttt attaataacc aaataaggct
    58321 gaagactttc taaaacatta tctcttctgt tcagtatact gattattgta ttaacattag
    58381 tgtcaagttg ctcattaaag ttctccctta gcaatacgtt gagaacttca tcttctatat
    58441 taagcaactg gctaaggaaa ttttctcctt cagccatgat tcccaaatgt cctgttgcaa
    58501 aaagcgcgta caaaactgca ccgactgagc catcggtatc gccaaaatag gtcggcctgg
    58561 tacttgtggg atcaacaggt gaacaattgg agaaataggt tctgtcagcg aactggggtt
    58621 ttataattgt ttcgaattta atatcaaata cattgtattg cgatatttcc cagttccaga
    58681 tgggtttgta cgactcagta actgcaacat cattagcatt atcatttact gtcatattat
    58741 taagtaaata aacaatgctg caccaattaa tatcggtatt atcaagaggg caaccgtaat
    58801 ttttactatt aattgtcaca ataaactgat aacagttatt cctacgtaga atatgagcgc
    58861 gcatgcccga tagcatacta tatttttctg aatgattcgt ttgtatggta aatgaaattt
    58921 tgtcatcttg aatagattgt tggaaacgtt ctatttgcca atcatcaatt ttttccacaa
    58981 cttcaccgag cattgtcctg gcaatacttg atatatcatc acctatacca tttgcaatat
    59041 acccaaacag ttctttagtg aagttggtaa catgctcttg caccatatca tttgttgcga
    59101 ttgtcgttgt actgccatta gtgcctgcca cagttccaat tttcatcttt tacctcatat
    59161 tattcaccgc gaggaataca tagttaacat tttatcttat gacatacacc tcatattcga
    59221 tggggaaata attatatttt gcacaaatca ttcagcctta aagcataata tgaaatttct
    59281 tatctcatag cagagaaagt ccctaaatat tgtagaggcg tcatcagtac atacctctac
    59341 aatcgattaa gaaaacctat gaaatactgg caaaattaaa aagagatacc acagcactca
    59401 tgaagtttta ttgaggttga aaacaactaa gccctgggac acctttttta tcaccacgtt
    59461 cgttaggtgc tatcttccaa gaatttagcc ccatggtata attatcaatt ttttgatgct
    59521 ggtctacccc taatccataa tggccaaatt tctgtttagt ttttttgagc gtctgtccac
    59581 atttgaagat atcttcaggt tttggtctga aagcagtagt gttattaaaa taattttcat
    59641 atacaggtgt gggtgctata gcattgcttc taattagatg gagaagatga tcatccatgt
    59701 gttgtttatg ttttttatta catatatttg ctaattcctc aggatcaatt gggtctttca
    59761 tctgattaaa tggaacctgc aaagcaccga gacaataaca ttgattttct tcatttctta
    59821 ttattccgtg atgactggtt aaaactttag tttctgagtt ataattacaa acatcgagcg
    59881 ttttaattaa gttatacgta tcaccggcag agagtttata attttctagt cgtgcagtat
    59941 agttaccatt aaaattgatt tcatgatttc cagccagaac gatgacattt ttattaatac
    60001 ggctatcttt attaccctcc atatttcgca tagaatttag caaagtaagg atatatttat
    60061 caccaaaaat agtactaaat ctatcgcctg tatgatcccc aaggaagata caaggggtta
    60121 tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatcatg tctctcgtat
    60181 ttaataaatt gtagagcaat ttattatttt tatgtatgta tgcagatgaa gccatgtctt
    60241 cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct atatgccctg
    60301 aaacgagcaa tgaataaagt acagcgccta ctcttccatc tgtatcgcca aaataagccg
    60361 tgttggcatt ttgtgggtta atcacaccac attgtttata aaagtctatt ttagaaaaca
    60421 cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag acatcccaac
    60481 gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttcaggt atgatagaaa
    60541 gcccattaac tttataaatt atgtctgatt tatctccgga gaaattaata aaataataac
    60601 catccttaca ctcaatctta actgtaatgg gagacgataa ttttatcaca tggttattgt
    60661 ctggtataat tgtaaagata aacgtgttag tagtattatc ggcaaaacta ttatattctt
    60721 gaatatctat agatggctca ctattgtaaa accatatttt ctccatctga agactatgtt
    60781 catttaccct ccagtgacca tgttgcagga aatagatgat atgcatgcca aaatcgttat
    60841 ctatttgcga atcattatcc agcttatcaa tttcaactat ctttgaacga tgtgttttat
    60901 caaccttgct atggtgttga tgattattaa ctttatctga atgcttatct ttatggtttg
    60961 aagggatgta cttaaatata ttgtcaattg ctttcataaa taacccgcga tatttttgac
    61021 ctgttagtaa aaaataaatg atacatgtct gaaagacata tttacgacga tataaaacaa
    61081 taatacttat atgttcgctg attttaagca ccgagactat ccggtctttt actaaataat
    61141 ggaaaacaat ccatccctgg aagacaattg taaaaaaaaa caacagaata agaattaatc
    61201 ttaggataaa tatttattta tcatggcctt ttgaccgcta aaaaatggca ctcggcacag
    61261 taaaccccgc tatagttatg ctatgcattt gtctttaatg atatctcgtc gttatattaa
    61321 caggctgaaa ttatcattaa tgcacatttc aatattagca gggatacctg aggagtttaa
    61381 agtgaacata tatatcgggt agcttttcaa attaatccct ttgattatag gcttaatttg
    61441 catcgcgtta ggtggctttg tgctggaaag ttcagggcaa cgtcacgggt catgtgctga
    61501 tttctctggc ggccatatgc ctggcattat tcactaccgc atttattatc atttcgcaac
    61561 tcacgcgcgg cgttaatacg ttttacaata cattgttccc cattattggc tatgcggggt
    61621 caattatcac catgatatgg ggttgggcac tgttagcagg taatgatgtg atggcagacg
    61681 agtttgttgc cggccatgtt atttttggcg ttggtatgat tgccgcatgt gtatcgacgg
    61741 tggcagcgtc gtccggtcac tttctgctca ttcccaaaaa tgcagcgggg agcaagagcg
    61801 acggaacacc ggtacaggct tattcttcat taatcggtaa ttgcctcatt gccgttcccg
    61861 ttttactcac cctgctcggt ttcatttggt ctattacgct gttacatagt gccgacataa
    61921 ctccgcatta tgtcgccttg ggttaaccgc aatctgtgcc tgtctaattg gcctagtggc
    61981 cacaatcgtc catcaaacac gtaatacgtt ctcatccaaa gagcactggc tgtggtgtta
    62041 ttgggttatt tttctcggct cagtcacggt gctgcagggg atatacgtct tagtcagttc
    62101 cgatgcaagc gcccgactgg ctcccggcat tattctcatt tgcctcggaa tgatctgtta
    62161 cagcatattc tcaaaagtct ggctactggc actggtctgg agacgtacct gttcgttagc
    62221 caacagaata ccgatgatac cggtatttac ctgtctgttt tgcttgtttt tgtcatcatt
    62281 cctcgcggaa atcgcacaaa ctgagctgga ctactttatt ccatctcggg ttctggttgg
    62341 attaggcgca gtatgtttta cattgttctc tattgtttca atattagagg ccggttctgc
    62401 gaaaaaaaat taaaatttac ataatcccag cgttattcat atcttaacgc tggtttttta
    62461 cgaacttagt gaaataatgt agaatataaa attataattt tatattggag cacatgtgtt
    62521 aatgatcaac cccgggatac taaaaatata gacattatta aacttgctta gcctcgcatt
    62581 gcctttacct gttaaagatg catcgagaaa attgagtctg aagttctcat cctttatctg
    62641 attttccagt cccgaaactt tggccttagc attacctgag atccgtccgt tactatctat
    62701 tttgtaggta aaaaaatacg aatgggaaaa tttcctcggt gtatcttcat cagttaagcc
    62761 aacaatcgta aaactaccgc taccatcttt ttttaaaatg actgaaaggt tagcagagta
    62821 gtataaagaa tgatctgttt tagcatttct tgttaacgtc gacgtacatt caagagacgt
    62881 tgtgctacca tcacgagcct tccagagagc aaaaaaacac acctgtagca atgtgaaaac
    62941 aagtattaat ataaaataca ttttcttatt agtcatataa tagcacctct gaacaaattg
    63001 cataagtttc gtcattagag gattttatac atcttgataa ataccgctgc ccttccccaa
    63061 gcaaataatg catactattt acatcatata taaaatttgc acctggcaca cagggtaaat
    63121 gctttttcga aatttcagcg gcctctattt gagatttagc gatgccaatc tcgttaagat
    63181 tctctgaaag atataaatca caggtgtcaa tcttacctga tttaaccaat ggataatccg
    63241 ttatactgta ggtatcttta aaagccacgg caaacatttc aataataaca attactacca
    63301 caagcgaaat caaaatgcga aaaagaggaa tactcctttt gcgctctact ttttcaacaa
    63361 catcttcttg taaatgagaa tcatcataag attgtttact tattgttatg ttttcactga
    63421 tgtaaaagcc tactttgggt atcgtaagaa tcacattttc ttcaacacca atatttttaa
    63481 aacttttgcg taacagggat atatattggt tcaatgtgtt gtttgacaga tcaaatgaaa
    63541 atctctccaa aacgctatca atgattgagt tcctgtcaac cgctccacca ttactttcca
    63601 gtaacactgc caaaatacac cccgccgtag ctggaaaatt tatctcatcc tgggtattta
    63661 cattcgagag cgagcatttg ttcggatcaa aaataatcgc gtcattgata atgaaaaaac
    63721 atctccgctc catgcaggca acaacacaaa ataagatttt gtcattttag cctattcctg
    63781 gtggaactga cacaagaaac taaaatacaa atataaccct gaaaaagtca tataaaactt
    63841 atatccacag aataaaaaat caaacatgtc agttttatca atataaagta tcgcaaggcg
    63901 aaaagtgatg caagaatact cctaagtaaa tgaaagcgca ccacatgaaa ttatcaacac
    63961 atgtaatttc atcattaggt gctatcgaaa ataactcagg agctacttaa gatgaataat
    64021 gtcttactca tgagacccga tgactcagaa tttatgatac ctgatgactg gtcagggcta
    64081 aacgcaaaag gcattacata cgaacaagat aatagagtga cagagtcttt tctgcctttt
    64141 aaatcttttt attgttcacc ggtgtgcacg acagttgaac tcatctcata aaataattgt
    64201 tttgctatgg gtatccgcta ccttctggaa aagaaaagag acttaaggct aaatcacttt
    64261 aaagccttct ctcataaatg cgagacaaaa cctgacgtgt taattatgac cgggtgcacc
    64321 gtcgctgata cttatagtat attctcacgc attgttgaag aaaatataat gaaaacgatg
    64381 acacctatcg tcatcattac tgggcatcac tcagaagatc tttgttctct ggcatcgaaa
    64441 ctgggaggag ttaaaataat atcaccgaca gaaaagattg caaaaataga aaacgctatc
    64501 aatgatgctt ttaacagcaa gcctaaaaaa ataagatttt taacagatat actctctcct
    64561 caacaaattg agaccatgaa aatggttgcg cagggtagat ccgttaatga gatttcagcc
    64621 gcaatgaata ttagcatcaa gaccgtgtat gcacataaag ttcgtgctat caataagctg
    64681 ggaattaaga aaaaagttta tgaagcgctt ttttataaag cgctaattca gttcaactga
    64741 tgtaaaaatc accctcgctt aataaataat atattctatg tcttttttta taagttaaaa
    64801 aaggcgggat atcaaatccc gccatgaaat taactaatca tctgcattat tctgcgtaga
    64861 tataagcctg ggtacggatg gtgtaagagt ctggctcaac aacctgttct ttcatatggg
    64921 aaacgacgcg ataggcacca cctgttccag tatttttcag catcatataa cgcttaccgc
    64981 caatctcttc ggaggcagct gaaggaggat cctgctcaag cgttgtcagg gaaactttca
    65041 gtttatggtc cgggttattg tcaccagaaa ttactgcatc cgctgaagta ctgctctccg
    65101 cataaatatc atccgggaaa gttacagcat agtcgtggct ctgcccatca gatgcgccaa
    65161 caatacctgt tgccaaaggc gtccatgcag ttaacgtacc gctatatacc cctttcactg
    65221 gttgaatggt attattaaca acgataggct tggtcaaagt aagattagct gacgcttcag
    65281 taccctggct tactaaagtt cccgcctgag aagcaaaagc tgtccccgcc agcatgattg
    65341 ctaacaatgt tttcttcatt atattttctc catgattttc atttacactg caaaatgcag
    65401 tttctttctt tgcatttacg tgctatcgcg aaaatgcttc caccaaataa tggaaacatc
    65461 ttcacaaaaa taaattatga gtttcttaat ttagaaacta taaacatgtc gccgctatct
    65521 ggtataaccc tgatgcactt ttcttcgcag gaagttggta ttttgcgata caggattgat
    65581 ctttagaata accccatttc acattaaact cgccttccgc actcaagcca gaaacataag
    65641 caataccatc ttcacctaca atgccggcca tattttcagt gtctttgcta taactaatca
    65701 cagcgccgaa ggggatgggt ttaccctctt tctgttttaa ggttactaat cctttatccc
    65761 cgctcttcac tgccaatgtc gcaggcacaa cggctccagc tgtcggtaca accaattgac
    65821 tagtttgtgg taagtcgtaa ttcttaccta tcgttttttc atccagaata acatcgtctc
    65881 ttcggtatgg ctgcactggc agtattgcat acccacgaaa atcagtactc agtcccggcc
    65941 agccattaac gttaaccccc gttgctccgg gtgcctctat aagggctaat gtcccatcca
    66001 cattttgacc aaatgtaagt ccatcccttg taataagtat cccaccgctt aaatttgcac
    66061 ctagctgttt catgttttcg ctggagctgt aatggctact aaactgacca tatgatccat
    66121 ttttgccaac ttgcacaatg ttcatcgtgc tcttcggttg tgactgatga gcgttagtta
    66181 tatttgtcgc tacatcccac catacaccac cactattctg acctgataat gaaacctgat
    66241 agttattacc ctgctctccc gaacgactca tctgtgaaga cgcataaatt tgattttcgt
    66301 cacccagcga taatggcact gaaataccca cgcttaattt ccgatctact ttccatttat
    66361 gcgatgtcag caagtcgttt tttgcccagt taatggagaa tgttatgcct ctccataccg
    66421 tactataact caggctggta gaccgcgtgt ttcctgtgcc ccgggaagaa ttatacctcg
    66481 tggtattgaa gttcaaattc ccgatattac caaggggctg ggataaggat atcgtagagc
    66541 catcgtgact attcttatct ctttttctgt tccgttcatc ctgtgcactt actgcttcag
    66601 tcagtgtggc ataacctgat gcataacggt tcgtggttat agaca
//
LOCUS       DAJTRH010000009        65685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTRH010000009 DAJTRH010000000
VERSION     DAJTRH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(125..604)
                     /gene="folK"
                     /locus_tag="OO339_000657"
     CDS             complement(125..604)
                     /gene="folK"
                     /locus_tag="OO339_000657"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5925397.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(601..2019)
                     /gene="pcnB"
                     /locus_tag="OO339_000658"
     CDS             complement(601..2019)
                     /gene="pcnB"
                     /locus_tag="OO339_000658"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5925398.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2058..2984)
                     /gene="gluQRS"
                     /locus_tag="OO339_000659"
     CDS             complement(2058..2984)
                     /gene="gluQRS"
                     /locus_tag="OO339_000659"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5925399.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3021..3476)
                     /gene="dksA"
                     /locus_tag="OO339_000660"
     CDS             complement(3021..3476)
                     /gene="dksA"
                     /locus_tag="OO339_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5925400.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3654..4358)
                     /gene="sfsA"
                     /locus_tag="OO339_000661"
     CDS             complement(3654..4358)
                     /gene="sfsA"
                     /locus_tag="OO339_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5925401.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4373..4903)
                     /gene="thpR"
                     /locus_tag="OO339_000662"
     CDS             complement(4373..4903)
                     /gene="thpR"
                     /locus_tag="OO339_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5925402.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4977..7406
                     /gene="hrpB"
                     /locus_tag="OO339_000663"
     CDS             4977..7406
                     /gene="hrpB"
                     /locus_tag="OO339_000663"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5925403.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7602..10136
                     /gene="mrcB"
                     /locus_tag="OO339_000664"
     CDS             7602..10136
                     /gene="mrcB"
                     /locus_tag="OO339_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5925404.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10356..12599
                     /gene="fhuA"
                     /locus_tag="OO339_000665"
     CDS             10356..12599
                     /gene="fhuA"
                     /locus_tag="OO339_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5925405.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12650..13447
                     /gene="fhuC"
                     /locus_tag="OO339_000666"
     CDS             12650..13447
                     /gene="fhuC"
                     /locus_tag="OO339_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5925406.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13447..14337
                     /gene="fhuD"
                     /locus_tag="OO339_000667"
     CDS             13447..14337
                     /gene="fhuD"
                     /locus_tag="OO339_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5925407.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14334..16316
                     /gene="fhuB"
                     /locus_tag="OO339_000668"
     CDS             14334..16316
                     /gene="fhuB"
                     /locus_tag="OO339_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5925408.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16351..17631)
                     /gene="hemL"
                     /locus_tag="OO339_000669"
     CDS             complement(16351..17631)
                     /gene="hemL"
                     /locus_tag="OO339_000669"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5925409.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17856..19277
                     /gene="clcA"
                     /locus_tag="OO339_000670"
     CDS             17856..19277
                     /gene="clcA"
                     /locus_tag="OO339_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5925410.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19301..19366
                     /locus_tag="OO339_000671"
     CDS             19301..19366
                     /locus_tag="OO339_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925411.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19359..19703
                     /gene="erpA"
                     /locus_tag="OO339_000672"
     CDS             19359..19703
                     /gene="erpA"
                     /locus_tag="OO339_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5925412.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19750..20373)
                     /locus_tag="OO339_000673"
     CDS             complement(19750..20373)
                     /locus_tag="OO339_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5925413.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20411..21211)
                     /gene="btuF"
                     /locus_tag="OO339_000674"
     CDS             complement(20411..21211)
                     /gene="btuF"
                     /locus_tag="OO339_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5925414.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21204..21902)
                     /gene="mtnN"
                     /locus_tag="OO339_000675"
     CDS             complement(21204..21902)
                     /gene="mtnN"
                     /locus_tag="OO339_000675"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5925415.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21986..23503
                     /gene="dgt"
                     /locus_tag="OO339_000676"
     CDS             21986..23503
                     /gene="dgt"
                     /locus_tag="OO339_000676"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5925416.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23633..25057
                     /gene="degP"
                     /locus_tag="OO339_000677"
     CDS             23633..25057
                     /gene="degP"
                     /locus_tag="OO339_000677"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5925417.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25212..26368
                     /gene="cdaR"
                     /locus_tag="OO339_000678"
                     /pseudo
     CDS             25212..26368
                     /gene="cdaR"
                     /locus_tag="OO339_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26457..26843)
                     /locus_tag="OO339_000679"
     CDS             complement(26457..26843)
                     /locus_tag="OO339_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5925418.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27157..27981)
                     /gene="dapD"
                     /locus_tag="OO339_000680"
     CDS             complement(27157..27981)
                     /gene="dapD"
                     /locus_tag="OO339_000680"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5925419.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28012..30684)
                     /gene="glnD"
                     /locus_tag="OO339_000681"
     CDS             complement(28012..30684)
                     /gene="glnD"
                     /locus_tag="OO339_000681"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5925420.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30746..31540)
                     /gene="map"
                     /locus_tag="OO339_000682"
     CDS             complement(30746..31540)
                     /gene="map"
                     /locus_tag="OO339_000682"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5925421.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31908..32633
                     /gene="rpsB"
                     /locus_tag="OO339_000683"
     CDS             31908..32633
                     /gene="rpsB"
                     /locus_tag="OO339_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5925422.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32891..33742
                     /gene="tsf"
                     /locus_tag="OO339_000684"
     CDS             32891..33742
                     /gene="tsf"
                     /locus_tag="OO339_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5925423.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33889..34614
                     /gene="pyrH"
                     /locus_tag="OO339_000685"
     CDS             33889..34614
                     /gene="pyrH"
                     /locus_tag="OO339_000685"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5925424.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34906..35463
                     /gene="frr"
                     /locus_tag="OO339_000686"
     CDS             34906..35463
                     /gene="frr"
                     /locus_tag="OO339_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5925425.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35555..36751
                     /gene="ispC"
                     /locus_tag="OO339_000687"
     CDS             35555..36751
                     /gene="ispC"
                     /locus_tag="OO339_000687"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5925426.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36940..37698
                     /gene="ispU"
                     /locus_tag="OO339_000688"
                     /gene_synonym="uppS"
     CDS             36940..37698
                     /gene="ispU"
                     /locus_tag="OO339_000688"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5925427.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37711..38568
                     /gene="cdsA"
                     /locus_tag="OO339_000689"
     CDS             37711..38568
                     /gene="cdsA"
                     /locus_tag="OO339_000689"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5925428.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38580..39932
                     /gene="rseP"
                     /locus_tag="OO339_000690"
     CDS             38580..39932
                     /gene="rseP"
                     /locus_tag="OO339_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5925429.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39962..42394
                     /gene="bamA"
                     /locus_tag="OO339_000691"
     CDS             39962..42394
                     /gene="bamA"
                     /locus_tag="OO339_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5925430.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42516..43001
                     /gene="skp"
                     /locus_tag="OO339_000692"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42516..43001
                     /gene="skp"
                     /locus_tag="OO339_000692"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5925431.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43005..44030
                     /gene="lpxD"
                     /locus_tag="OO339_000693"
     CDS             43005..44030
                     /gene="lpxD"
                     /locus_tag="OO339_000693"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5925432.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44135..44590
                     /gene="fabZ"
                     /locus_tag="OO339_000694"
     CDS             44135..44590
                     /gene="fabZ"
                     /locus_tag="OO339_000694"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5925433.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44594..45382
                     /gene="lpxA"
                     /locus_tag="OO339_000695"
     CDS             44594..45382
                     /gene="lpxA"
                     /locus_tag="OO339_000695"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5925434.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45382..46530
                     /gene="lpxB"
                     /locus_tag="OO339_000696"
     CDS             45382..46530
                     /gene="lpxB"
                     /locus_tag="OO339_000696"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5925435.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46527..47123
                     /gene="rnhB"
                     /locus_tag="OO339_000697"
     CDS             46527..47123
                     /gene="rnhB"
                     /locus_tag="OO339_000697"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5925436.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47160..50642
                     /gene="dnaE"
                     /locus_tag="OO339_000698"
     CDS             47160..50642
                     /gene="dnaE"
                     /locus_tag="OO339_000698"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5925437.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50655..51614
                     /gene="accA"
                     /locus_tag="OO339_000699"
     CDS             50655..51614
                     /gene="accA"
                     /locus_tag="OO339_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5925438.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51713..53854
                     /gene="ldcC"
                     /locus_tag="OO339_000700"
     CDS             51713..53854
                     /gene="ldcC"
                     /locus_tag="OO339_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5925439.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53911..54300
                     /locus_tag="OO339_000701"
     CDS             53911..54300
                     /locus_tag="OO339_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5925440.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54365..55663
                     /gene="tilS"
                     /locus_tag="OO339_000702"
     CDS             54365..55663
                     /gene="tilS"
                     /locus_tag="OO339_000702"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5925441.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPCADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55712..55972)
                     /gene="rof"
                     /locus_tag="OO339_000703"
     CDS             complement(55712..55972)
                     /gene="rof"
                     /locus_tag="OO339_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5925442.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55959..56159)
                     /locus_tag="OO339_000704"
     CDS             complement(55959..56159)
                     /locus_tag="OO339_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5925443.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56325..56870
                     /locus_tag="OO339_000705"
     CDS             56325..56870
                     /locus_tag="OO339_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5925444.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56867..57289
                     /gene="arfB"
                     /locus_tag="OO339_000706"
     CDS             56867..57289
                     /gene="arfB"
                     /locus_tag="OO339_000706"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5925445.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57303..58013
                     /gene="nlpE"
                     /locus_tag="OO339_000707"
                     /gene_synonym="cutF"
     CDS             57303..58013
                     /gene="nlpE"
                     /locus_tag="OO339_000707"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5925446.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58168..58992)
                     /locus_tag="OO339_000708"
     CDS             complement(58168..58992)
                     /locus_tag="OO339_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5925447.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59045..60763)
                     /gene="proS"
                     /locus_tag="OO339_000709"
     CDS             complement(59045..60763)
                     /gene="proS"
                     /locus_tag="OO339_000709"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5925448.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60874..61581)
                     /gene="tsaA"
                     /locus_tag="OO339_000710"
     CDS             complement(60874..61581)
                     /gene="tsaA"
                     /locus_tag="OO339_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5925449.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61578..61982)
                     /gene="rcsF"
                     /locus_tag="OO339_000711"
     CDS             complement(61578..61982)
                     /gene="rcsF"
                     /locus_tag="OO339_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5925450.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62100..62915)
                     /gene="metQ"
                     /locus_tag="OO339_000712"
     CDS             complement(62100..62915)
                     /gene="metQ"
                     /locus_tag="OO339_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5925451.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62955..63608)
                     /gene="metI"
                     /locus_tag="OO339_000713"
     CDS             complement(62955..63608)
                     /gene="metI"
                     /locus_tag="OO339_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5925452.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63601..64632)
                     /gene="metN"
                     /locus_tag="OO339_000714"
     CDS             complement(63601..64632)
                     /gene="metN"
                     /locus_tag="OO339_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5925453.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64820..65395
                     /gene="gmhB"
                     /locus_tag="OO339_000715"
     CDS             64820..65395
                     /gene="gmhB"
                     /locus_tag="OO339_000715"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5925454.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15532 a        16276 c        18095 g        15782 t
ORIGIN      
        1 gattgatatt tatctgcggg aatttatatt atgtttctgc aaaaatgttt aaactaaaat
       61 ttttattaag gaaagagagg atgtatgatt ttcagaaaat atttatattc gtaatataaa
      121 taaattacca tttgcttaat ttgtcaaatg ctcttgtatg taagacttca cgcaacgtct
      181 ccccatcagg aaacgccagc tccggtgcaa tctcaaacag cggccacagc ataaatccac
      241 gattcttcat atcgtagtgc ggaacggtca ggcgttcggt atttatcact tcattaccaa
      301 acagcatgat atcgagatcc agcgtgcgtg gtccccagcg ttcagcttta cgaacacgac
      361 cctgttgcag ttctatgcgc tgtgtatggt tgagtaactc ttccggggcg ggcgaagttt
      421 caagcgccac ggcggcgttg aggtaatcgg gttgatcttg cggccccagc ggtggggtgc
      481 ggtaaaacga agaaacggca agaatgcggc tttcagggat atcgcctaat gctttcaggg
      541 cagcattgac ctgctccagc ggagaggcca gattgctgcc tatggcaata tacgccactg
      601 tcatgtggta ccctcacgac gcggtgcgcg tttgcgtgga cgacgagtac gacgacgcgg
      661 tgacggttct tcatccagct cgttaagcat ccctttttga tctggtggtg cggaaacctg
      721 gaactcaccc caccatttca ccagacgctg cagttcagca ttacgttcaa cttcagctcg
      781 caaggccaac aggtcataag ccgcacggaa cttaggatgc tccagcagtt tccatgcgcg
      841 tttaccctga cgacgggaca tacgcaactg caactgccag atatcgcggg ttaacgtcgt
      901 cagacgtttc gggattgcca gtgaacggca ggcttcgtcc agcacgtcgt tcatcgccag
      961 cgcgaaagcg tcgtgatagg tcaggccgct ttcctgggcg atcttctgtg ccgtctccag
     1021 cagtgggtac cagaacatgg cggcaaacaa gaacgccggg ttcacgcgca tatcgttatg
     1081 gatacgcgta tcggtattct tcagcacctg ttcaatgatc cgctccatcg ggctgtcgcc
     1141 attttccgtg aagtagcggg taatggtcgg gaacagcggc tggaacagat gatattcaca
     1201 caacagctta taggtttcgt aaccgtagcc cgcttgtagc agtttaagcg attcttcaaa
     1261 caggcgtgcc ggtgggatat cgttcagcag ggtagcgagg cgagggatcg gttctgcggt
     1321 ttccgggctg atgcgcatac ccaatttggc ggcaaaacgt accgcgcgca gcatacgtac
     1381 cggatcttca cggtagcgcg tttccgggtt accaatcaga cggataacgc cgtccttcag
     1441 atccttcata ccaccaacgt aatcacggac ggtaaaatcc gctacgctgt aatacaggct
     1501 gttgatagtg aaatcgcggc gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg
     1561 cagcaacatg ccgttttgcc cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg
     1621 gtgtccacgg aaggtcgcaa cttcgataat ctccgggcca aacattacat gagccagacg
     1681 gaaacggcga cccaccaggc ggcagttacg gaacagtttg cgcacctgct caggcgtggc
     1741 gttagtggtt acgtcaaaat ctttcggctt tttgccaagt aacaggtcgc gcacgccgcc
     1801 gccaaccagc caggcttcgt atcccgcttt gttgagcctg tacattacct tcagggcatt
     1861 ttcactgata tctttgcggg aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg
     1921 acgggcgact gcctgttcag cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt
     1981 agcgactcgg gtaaaaatag tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa
     2041 tagcggctaa tcatagctca gcatgacgca tttgagaatg ttgaatttac aattgccgac
     2101 tcgggcacgg cggtcagcat ccagtttttg acggctgact gaaggatttg ctcgacgctg
     2161 aaatcctgcc agtgtgcttc tgcctgctgc cccagaaatt gaagtgccgc gattagtacc
     2221 gggcgtggat cgcctttcgg caacgcaggc gcatgattct gcttggaaag tttagcgcct
     2281 tgtggattaa gcgccagcgg cagatgaatg taatctggca ctttccagcc aaaaagctgg
     2341 tacagcggga tttgccttac tgttggttca atcagatcag ccccacgcac tatttctgta
     2401 acgccctgga aatgatcatc aaccacaaca gccaggttgt aggcgaacaa cccatcacgg
     2461 cgatgaatga taaaatcttc ccgtgccagt ttttcgtcgg cgtgaataat gcctcgcagc
     2521 aggtcagtaa attgcgtgac cggatgctgc tggcggatac gcactgcggc gttgtctggt
     2581 ccatgatgca acacccggca atgaccgtcg taaataccgc caatgctttg aatacgcgca
     2641 cgcgtacagg tgcagtaata acttagtcct tgttcatgta accaggcgag tgcttcacga
     2701 taggcgtcgt gacgttgcga ttgccagaga acatcgccgt cccagtgcag accgtaatgt
     2761 tccagctggc gcaggatagt ttctgcggca ccgggaactt cacgaggcgg gtcgatatct
     2821 tctatgcgta ccagccagcg accttgccgg gcgcgagcct gcaaatagct gccgagcgcg
     2881 gcgatcagag agccaaaatg aagctcgccg gaaggagagg gggcgaagcg gccaatatac
     2941 tgtgtgtctg tcatctcttt gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa
     3001 acgtgatgga acggctgtaa ttagccagcc atctgttttt cgcgaatttc agccagcgtt
     3061 ttgcagtcga tgcacagatc ggctgtcggg cgcgcttcca gacggcgaat accaatttca
     3121 acaccgcagg attcgcagta gccgaaatct tcgtcttcca cttttttcag cgtcttctcg
     3181 atctttttga tcagcttacg ctcgcgatcg cggttacgca gttcgaggct gaactcttct
     3241 tcctgggctg cacggtctac cgggtccggg aagttggctg cttcatcctg catatgtgta
     3301 acggtgcgat cgacttcatc cctgagttga ttacgccatg cttccagaat acgacggaag
     3361 tgcgccagct gggcttcatt catatactct tcgcccggct tctcctgata tggttccacc
     3421 ccagcgatgg cgagaatact cagggacgat gttttacggt tttgcccttc ttgcatgttg
     3481 cttctcctta acacgcacta tcgatcccca tgttcggggg aaaaatgagg ccgctataaa
     3541 tagcagatgc ttttccggat agcaattatc taaacgtaac acttgacaac tgtgtgagga
     3601 aaagcgtatt tgcgcacgcg accagaatgt aaattaacca gttacttact ttactacaat
     3661 gtaaccggca gtgatttttt aagagccatg ccttcagcag aaatttccgc tttgtaagcc
     3721 agaatttcta cccccctctg ttgagcttct gacaatagtt gcgcgtattt ctcatcgatg
     3781 tggcgcgcgg gtgaaaaccg tgtaatggct gaatgcagca cggcgaaaaa aataaccgca
     3841 cgctggcctt cagccgctac gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg
     3901 accgcatcgg gaaaatatcc ctgttcgttc tccgctaacg taaccgattt cacttcaata
     3961 tagcagtctg gacgcgaatc cgcctgcaac ataaagtcaa tacggctgcg ttcggagccg
     4021 tattttactt cgcttttcag cgagctatag cctgacagtt ctgaaattga ttcattaagg
     4081 atagcctctt tcgtcaacct gttagcccaa agcgtattga cgcaaataaa tgcgccgctc
     4141 tggctttgag ttaattccca ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa
     4201 taccagacgg tatcgccagg cgttgcacaa ccggtcatcg cacccgtatt cgggcagtgt
     4261 agcgttaatt cgcgaccatc gggtgtgatc acatcggcta aaaaacgttt gtaacgctga
     4321 attagcgtcg cgcgctgtag agggggagaa aattccattg cgacaatcct tgttattgcg
     4381 ttagcgccca gcgttttagc ggcgtgtagc gtgtgcgtcc acgggcaaac gaggaggcgt
     4441 aaagggtgaa ctccgtcacc gcataagacc agttaaaacc cggcggcggg aatgtcaccg
     4501 cctcgctggc gtcgcgcaat aaggtaatat gtggatgaaa cggacgattg ctttgaaaac
     4561 aaccgctgcg ggcagcctgt gaacggagca tattcgccag ctggattaag ccgcgtggag
     4621 gctgacgcat ccctaaccac accacacgcg aacgcagcca ttgtccggcg tcatcaagcg
     4681 tgagtgtgaa accaggttga cgaatccgtc cggctaaaag agaaagcgcc ttctctttct
     4741 ctgcgctcac ttcgcctaaa aatgccagag tcaggtgcaa attatcggcg gcgaccggac
     4801 gtcccgcctc aggtgggaag tgtgtggcgc gccaatggat aatctgttcg cggatttctg
     4861 caggtaagtc gatagcaaag aacagacgtt gcggttcaga catgtgaggc actcggttat
     4921 gaattaccgc gatgctacaa tgtggcgcga agaatgttaa ccctctggag cgttttgtgt
     4981 cgtcgttgcc cgttgctgcc gtcttacctg aattacttac cgctctcgat tgtgcgccgc
     5041 aggtattatt aagtgcgccg accggggccg ggaaatcaac ctggctgccg ctgcaactgc
     5101 tggcgcatcc cggcattaac gggaaaatta tcctgctgga gccgcgtcgt ctggcggcgc
     5161 gtaacgtcgc gcaacggctg gcggagctgc ttaacgaaaa gccaggcgat accgttggct
     5221 accggatgcg tgcgcaaaac tgcgtcgggc tgaatacccg cctggaagtg gttaccgaag
     5281 gcgtgctgac gcggatgatc cagcgtgacc cggaactgag cggtgttgga ttggtgatcc
     5341 ttgatgagtt tcatgagcgc agtttgcagg cggatttggc gttggcgctg ttactcgatg
     5401 tgcaacaagg tctgcgtgat gaccttaaac tgctgattat gtcggctacg ctggacaatg
     5461 accgcttgca gcaaatgctg ccggaagcgc ccgtcgtcat ctcagaaggg cgctcgtttc
     5521 cggttgaacg ccgttattta ccgctgcccg cgcatcagcg ttttgacgaa gccgttgcgg
     5581 tagccaccgc cgaaatggtg cgtcaggaaa gtggatcatt actgttattt ttacctggcg
     5641 tcggagaaat tcagcgcgtg caggaacaac tggcttcgcg catcggcagt gatgtggtgc
     5701 tctgcccgct gtatggcgtg ttgtcactga acgatcagcg aaaagcgatc ctcccggcac
     5761 cgcaagggat gcgcaaagtg gtgctggcga ccaatattgc tgaaaccagt ttaaccatcg
     5821 aaggtattcg tctggtggtg gattgtgccc aggagcgtgt ggcgcgcttt gatccgcgca
     5881 cggggcttac gcggttgatt actcaacgcg ttagtcaggc atccatgacg cagcgtgccg
     5941 ggcgcgccgg gcgtctggag ccgggtatca gcctgcattt aatcgccaaa gaacaagcag
     6001 aacgcgccgc ggcgcaaagt gagccgaaga tcttacaaag cgatctttcc ggtttgctga
     6061 tggaattact gcaatgggga tgcagcgatc cggcgcagat gagctggctg gatcaaccgc
     6121 caacggtgaa tctactggcc gcgaaacgcc tgttacaaat gctgggggcg ctggagggtg
     6181 aacggcttag tgcgcaaggg caaaaaatgg cagcgctggg taacgatccg cgtttagcgg
     6241 caatgctggt taacgcaaag agcgatgacg aagctgctac cgcggcaaaa attgccgcca
     6301 ttctcgaaga gccgccacgg atgggcaata gtgacctggg cgtggcgttt tcgcgcaatc
     6361 aaccagcctg gcagcaacgt agtcagcaac tgttaaaacg cttaaacgta cgcggcggtg
     6421 aggcagacag ttcgcttatc gcgccgctac ttgccggggc gtttgccgat cgcattgctc
     6481 atcgccgtgg gcaagatgga cgctatcaac tggcaaacag catgggagcg atgctcgatg
     6541 ccgacgacgc gctaagccgc cacgaatggt tgatcgcacc gttattattg cagggcagcg
     6601 cctcgccgga tgcgcggatt ttactggcgc taccggtcga tattgatgaa ttagtacaac
     6661 gctgtccgca gctggtgcag caatctgaca ccgtggaatg ggatgacgcg caaggtacgc
     6721 tgaaagcctg gcgtcggcta caaatcggtc agttgacggt gaaagtgcag ccgctggcga
     6781 aaccgtcaga agacgagttg catcaggcga tgcttaatgg catccgtgat aaaggtttaa
     6841 gcgtgctcaa ctggacggcg gaagcggaac agctacgctt gcgtttgtta tgcgccgcaa
     6901 agtggttgcc ggaatatgac tggccagcgg ttgatgatga aagtttgttg gcgacgctgg
     6961 aaacgtggct gctgccacat atgaccggcg tacattcact acgcggcctg aaatcactcg
     7021 acatttatca ggcactacgc ggattacttg attggggaat gcagcaacgt ctggatagtg
     7081 aattgcctgc gcattacact gtgccgacgg gaagccggat cgccattcgt tatcatgaag
     7141 ataacccgcc cgcgctggcg gtgagaatgc aggagatgtt tggtgaggcc actaatccga
     7201 cgatcgccca ggggcgcgtg ccgctggtgc tgaagttgct ttcccctgcc caaaggccgc
     7261 tgcaaatcac gcgtgatttg ggggccttct ggaaaggggc gtatcgtgag gtgcaaaagg
     7321 agatgaaagg gcgttatccc aaacacgtct ggccggacga cccggcaaac acggcaccga
     7381 cgcgacggac gaaaaagtat tcgtaaggtt ggttttctcc ctctccctgt gggagagggc
     7441 cggggtgagg gcatcagcgc gcacgttcac cctcacccta accctctccc tcaagggaga
     7501 ggggaccgat cgagcgcaaa ttttgagaga tatcttcttc tgtcttgtaa cagaagaaca
     7561 gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg aatgaccgcg
     7621 agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc
     7681 gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa gaaccgatgc
     7741 cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat
     7801 ggctactgct aaaactggct atcgtttttg ccgtgctgat cgccatttac ggcgtttatc
     7861 tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgccc gcggcagttt
     7921 atggccgaat ggtcaacctt gaaccagata tgaccatcag caagaacgaa atggtgaagc
     7981 tgctggaggc aacacagtat cgtcaggtgt cgaaaatgac ccgtcctggc gaatttaccg
     8041 tgcaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac agtaaagaag
     8101 ggcaggtgcg tgcgcgtctg acctttgatg gtgatcatct ggcgaccatc gtcaatatgg
     8161 agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc atgatctcct
     8221 cgccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg tttccctgat ttgctggtgg
     8281 atactttgct ggcgacagaa gaccgtcatt tttacgagca tgatggaatc agtctctact
     8341 ccattgggcg tgcggtgctg gcaaacctga ccgcaggtcg cacggtgcag ggtgcgagta
     8401 cgctgacgca acagctggtg aaaaacctgt tcctctccag cgagcgttct tactggcgta
     8461 aagcgaacga agcttacatg gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc
     8521 ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct
     8581 tcccgctggc aagcttgtat tactttggtc gcccggtaga agagctaagc ctcgaccagc
     8641 aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta caacccgtgg cgtaacccga
     8701 aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa cagattattg
     8761 atcaagaact ctatgacatg ttgagtgccc gtccgctggg ggttcagccg cgtggtgggg
     8821 tgatctctcc tcagccagcc tttatgcagc tggtgcgtca ggagttgcag gcaaaactgg
     8881 gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac tcggtggccc
     8941 aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa cagcgtaagt
     9001 tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa gttcgtgcga
     9061 tggtcggagg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt
     9121 cgattggttc ccttgcaaaa ccagcgactt atctgacggc tttaagccag ccgaaaatct
     9181 atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct gcgtcagccg aatggccagg
     9241 tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag cggcagagtg atgctggtgg
     9301 atgcgttgac acgttcgatg aacgtgccga cggtaaatct ggggatggcg ctggggctgc
     9361 ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa agatcagttg catccggttc
     9421 cggcaatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag gcattccaga
     9481 ccatcgccag cggtggtaac cgtgcaccgc tttctgcact gcgttcggta atcgcggaag
     9541 atggtaaggt gctgtatcag agcttcccgc aggcagaacg cgctgttccg gcgcaggcgg
     9601 cgtatctgac actatggacc atgcagcagg tggtacaacg tggtacgggt cgtcagcttg
     9661 gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac aataacgtag
     9721 atacctggtt tgcaggcatt gacggcagca cggtgaccat cacctgggta ggccgtgata
     9781 acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat cagcgttatc
     9841 tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt gcagatatgg
     9901 gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg ccggtctgga
     9961 ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg tcaggcaatc
    10021 cgtttgatca gtcttctcag ccgcagcaac agccgcaaca gcaacctgct cagcaagagc
    10081 agaaagacag cgacggtgta gccggttgga tcaaggatat gtttggtagt aattaacatc
    10141 taagcgtgaa ataccggatg gcgagttgcc atccggtaaa ataacatccc atctaagata
    10201 ttaacccttt cttttcatct ggttgtttat taacccttca ggaacgctca gattgcgtac
    10261 cgcttgcgaa cccgccagcg tttcgaatat tatcttatct ttataataat cattctcgtt
    10321 tacgttatta ttcactttac atcagagata taccaatggc gcgttccaaa actgctcagc
    10381 caaaacactc actgcgtaaa atcgcagttg tagtagccac agcggttagc ggcatgtctg
    10441 tttatgcaca ggcagcggtt gaaccgaaag aagacactat caccgttacc gctgcacctg
    10501 cgccgcaaga aagcgcatgg gggcctgctg caactattgc ggcgcggcag tcagctaccg
    10561 gcactaaaac cgatacgccg attcaaaaag tgccacagtc tatttctgtt gtgaccgccg
    10621 aagagatggc gctgcatcag ccgaagtcgg taaaagaagc gcttagctac acgccgggtg
    10681 tctctgttgg tacgcgtggc gcatccaaca cctatgacca cctgatcatt cgcggttttg
    10741 cggcagaagg ccaaagccaa aataactatc tgaatggcct gaagttgcag ggcaacttct
    10801 ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc tgaaattatg cgtggcccgg
    10861 tttccgtgct ttacggtaaa agcagtcctg gcggtctgtt gaatatggtc agcaagcgtc
    10921 cgaccaccga accgctgaaa gaagttcagt ttaaagccgg tactgacagc ctgttccaga
    10981 ctggttttga ctttagcgat gcgttggatg atgacggtgt ttactcttat cgcctgaccg
    11041 gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga agagcagcgt tatgctattg
    11101 caccggcgtt cacctggcgt ccggatgata aaaccaattt caccttcctt tcttacttcc
    11161 agaacgagcc ggaaaccggt tattacggct ggttgccgaa agagggaacc gttgagccgc
    11221 tgccgaacgg taagcgtctg ccgacagact ttaatgaagg ggcgaagaac aacacctatt
    11281 ctcgtaatga gaagatggtc ggctacagct tcgatcacga atttaacgac acctttactg
    11341 tgcgtcagaa cctgcgcttt gctgaaaaca aaacctcgca aaacagcgtt tatggttacg
    11401 gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg tgcggcatta gcgccagcgg
    11461 ataaaggcca ttatctggca cgtaaatacg tcgttgatga tgagaagctg caaaacttct
    11521 ccgttgatac ccagttgcag agcaagtttg ccactggcga tatcgaccac accctgctga
    11581 ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc ctggtttggt tacgacgact
    11641 ctgtgccact gttcaatctg tacaatccgg tgaatcccga tttcgacttc aatgccaaag
    11701 atccggcaaa ctccggccct taccgcattc tgaataaaca gaaacaaacg ggcgtttatg
    11761 ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct aggcggtcgt tatgactggg
    11821 cagatcaaga atctcttaac cgcgttgctg ggacgaccga taaacgtgat gacaaacagt
    11881 ttacctggcg tggtggtgtt aactacctgt ttgataatgg tgtaacacct tacttcagct
    11941 atagcgaatc gtttgaacct tcttcgcaag ttgggaagga tggtaatatt ttcgcaccgt
    12001 ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc ggaagatcgt ccgattgtag
    12061 ttactggtgc cgtgtataat ctcactaaaa ccaacaacct gatggcggac cctgagggtt
    12121 ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg cgtagaaatc gaagcgaaag
    12181 cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac ttacaccgat gcggaataca
    12241 ccaccgatac tacctataaa ggcaatacgc ctgcacaggt gccaaaacac atggcttcgc
    12301 tgtgggctga ctataccttc tttgacggtc cgctttcagg tctgacgctg ggcaccggtg
    12361 gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg ggaagttata
    12421 cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg ggctccaacg
    12481 tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt cgccagctgc tttaacactt
    12541 atggctgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttt cgtttctaat
    12601 ttctcttttg gggcacggat ttccgtgccc atttcacaag ttggctgtta tgcaggaata
    12661 cacgaatcat tccgatacca cttttgcact gcgtaatatc tcctttcgtg tgcccgggcg
    12721 cacgcttttg catccgctgt cgttaacctt tcctgccggg aaagtgaccg gtctgattgg
    12781 tcacaacggt tctggtaaat ccactctgct caaaatgctt ggtcgtcatc agcctccgtc
    12841 agaaggggag attcttcttg atgcccagcc gctggaaagc tggagcagca aagcgtttgc
    12901 ccgcaaagtg gcttatttgc cgcagcagct tcctccggca gaagggatga ccgtgcgtga
    12961 actggtggcg attggtcgtt acccgtggca tggcgcgctg gggcgctttg gtgcggcaga
    13021 tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta aaaccgctgg cgcatcggct
    13081 ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg atcgccatgc tggtggcgca
    13141 ggatagccgt tgtctgttgc tcgacgaacc gacttcagcg ctggatatcg cccaccaggt
    13201 tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt ggcctgacgg tcattgccgt
    13261 gttgcacgat atcaatatgg cggcacgcta ctgtgattat ctggtcgccc tgcgcagtgg
    13321 tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc ggcgaaaccc tcgaaatgat
    13381 ttatggcatc ccgatgggta ttttgccgca tccggcgggt gctgcacctg tgagttttgt
    13441 ttattgatga gcggcttacc tcttatttcg cgccgtcgac tgttaacggc gatggcgctt
    13501 tctccgttgt tatggcagat gaataccgcc cacgcggcgg ctattgaccc caatcgtatt
    13561 gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt gccttacggc
    13621 gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac caccattgcc ggactcagtg
    13681 attgacgtcg gtttgcgtac agaacctaac cttgaactgc tgaccgaaat gaaaccgtcg
    13741 tttatggtct ggtcggcagg atatggccct tcatcagaaa tgctggcacg tattgcgccg
    13801 ggtcgcggat ttaacttcag tgacggcaaa catccgttgg cgatggcgcg taaatcgctg
    13861 acggaaatgg cagatttact taacctgcaa agcgcagcgg aaacgcattt agcgcaatac
    13921 gaagacttta tccgcagcat gaaaccccgc tttgtgaagc gtggtgcacg cccgttactg
    13981 ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg gtccaaacag cttgttccag
    14041 gaaattctcg atgagtacgg catcccaaat gcctggcaag gggaaaccaa cttctggggc
    14101 agtaccgccg tcagtatcga tcgtctggca gcgtataaag acgttgatgt gctctgtttt
    14161 gatcacgaca acagcaaaga catggatgcg ctaatggcaa ccccgctgtg gcaggccatg
    14221 ccgtttgtcc gtacagggcg ctttcagcgc gtacccgcag tctggtttta tggtgcaacg
    14281 ctttcggcaa tgcactttgt gcgtgttctg gataacgcca tcggaggtaa agcgtgagta
    14341 aacgaattgc gcttttcccg gcgttattgc tggcgctgtt agtgattgtc gctacggcgc
    14401 tcacctggat gaacttctca caggcgctgc cgcgcagtca gtgggcgcag gccgcctggt
    14461 cgccggatat tgacgtcatc gagcagatga tttttcacta cagcttgttg ccgcgtctgg
    14521 cgatttcgct gctggtgggc gcgggcctgg ggctggtggg cgtgctgttt cagcaagtac
    14581 tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc aacaggcgcg caactgggga
    14641 ttaccgtcac cacgctctgg gcgatcccta gcgcgatggc gagccagttt gctgcgctgg
    14701 caggggcttg cgttgttggc ttaattgtct ttggcgtcgc gtgggggaaa cggctgtcgc
    14761 cggtaacgct gattttcgcg gggttggtag tgagccttta ttgcggcgca atcaatcagt
    14821 tactggttat cttccatcat gaccagttgc aaagcatgtt tctgtggagc actggaacgc
    14881 tgacgcaaac cgactggggc ggtgttgagc gtttatggcc gcagttgctg ggcggtgtga
    14941 tgctgacgtt gctgctactt cgtccgttaa ccctgatggg gcttgatgat ggcgtggcgc
    15001 gcaatctcgg gctggccttg tcgcttgcgc gtctggcggc gctgtcgctg gcgattgtca
    15061 tcagtgcgct gctggtgaac gctgtgggga ttatcggctt tatcgggttg ttcgcgccgc
    15121 tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg actgatgctg gcgtcgttga
    15181 ttggtgcgct gatcctctgg ctttccgatc aaatcatcct ctggctgact cgcgtgtgga
    15241 tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc gccgctgcta ctgtggctgc
    15301 tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt taacgatcgt gtcgcgactg
    15361 aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt gctgttgttg atggctgtgg
    15421 tggtggcgct gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc ggggcgttgc
    15481 tcgaggattt aatgccctgg cgctggccgc gaattatggc ggcgctgttt gcgggcgtca
    15541 tgctggcggt agcgggctgt attattcagc gactgaccgg caacccgatg gcaagcccgg
    15601 aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt gttaatgctg tttctggtac
    15661 cgggtaatgc ctttggctgg ttgttacccg caggcagtct cggggcggcg gtgacgctgt
    15721 tgatcattat gatcgccgcc ggacgcggtg gattttcccc acaccgcatg ttactggcgg
    15781 ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat gttgcaggca agtggtgacc
    15841 cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg accgatgctc
    15901 aggtctggcg caccggaatt gtgatggtga ttttgctggc gattactccg ctgtgccgcc
    15961 gctggctgac cattttaccg ctgggtggtg ataccgcgcg ggcggtaggg atggcgctga
    16021 cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc gcgacgatga
    16081 ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg atgatgggct
    16141 ttcgacggac gatgccacac atcgtaattt cggcgctggt gggtggttta ctgctggtgt
    16201 tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccggcg gggctgctgt
    16261 cgacctttat cggtgcgcca tattttatct atttgctgag aaagcagagc cgttaataga
    16321 atgcctcgcc cggtgggcga ggcaaaatta tcacaacttc gcgaacaccc gacgtgcagc
    16381 atcgatggtg ttattgatat cttccatgct gtgcgccaca gacataaagc ctgcttcaaa
    16441 cgctgacggt gccaggtaaa caccttcatc cagcatcata tggaagaaac gcttaaaacg
    16501 ctcgacgtca caggccatca catcctgata gcacgtcacg gactcggcgt cggtaaagaa
    16561 gataccgaac ataccaccga cgtggttaac gaccagcgga attcccgctt cttctgccgc
    16621 ttccagcaga ccttctgcca gacgtgttgt cagctcatcc agcgtttcgt gaatgcccgg
    16681 ctgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaatcgggt taccggaaag
    16741 cgtacccgcc tgatagaccg gacctgtcgg ggccagcgca tccattacat cacgacgacc
    16801 accgaatgcg cctaccggca ttccaccgcc gatgattttt ccgaggcagg ttaaatctgg
    16861 cactacggcg aaataatcct gtgcgccagc tagcgctacg cggaaaccgg tcatcacttc
    16921 atcgatgatc agcaacgcgc caaattcgtc gcacagcgca cgcagacctg gcaggaactc
    16981 tggcagcggc ggaacacagt tcatattgcc tgccaccggc tcgacgataa tacaggcaat
    17041 ctcttgcggg tattgctcaa acgcggcgcg tacagaggcc agatcgttat aagtacaggt
    17101 taaggtatgt ttggcgaaat ctgccggaac gcccggcgag tttggctggc ctaacgtgag
    17161 tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac aaccttcaaa
    17221 tttaatgatt ttgtcgcgac cggtaaaacc acgggccagg cggatggcgc tcatcgtcgc
    17281 ctcggtgccg gagttcacca tgcgcaccat atccatggtc gggaccagtt cggtcaccag
    17341 ttgcgccatt ttcacttcca tttcggtcgg tgcaccaaag cttaaaccac gctcggcggc
    17401 ttcaatcacg gcattgcgga tcgccggatg gttatggccc agcaccatcg gcccccagga
    17461 accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc cgtccgcttt
    17521 ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg agttcacacc
    17581 gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca tggagggttc
    17641 ctgattcgta gaaaaagtga atggctgcta ttccatgtta ttcataacaa gttaaatact
    17701 cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta taaaagtttt
    17761 gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag taaaatgccg
    17821 tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact ccctctttag
    17881 aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt cttgagcgcg
    17941 ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt gtcgggctgg
    18001 ctgcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg ggggcgctgg
    18061 tacatactgc tgataattat ccgctactgt taaccgtcgc ttttctctgt tcggcggtgc
    18121 tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc cgaagcaggg ggatcgggga
    18181 tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt tcgctggtgg cgtgtattgc
    18241 cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggggcta gggcgcgaag
    18301 gaccaaccgt gcagatcggc ggcaatatca gtcgcatggt gcttgatatt ttccgcctga
    18361 aaggtgatga agctcgccat acgctgctgg caaccggtgc tgctgcgggg ctggctgcgg
    18421 cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt ccgcagtttc
    18481 gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg accattatgt
    18541 accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct gatgcgccgc
    18601 ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc ggccctattt
    18661 ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta
    18721 ccaaatgggt actgatgggg ggggcgattg gcggtctgtg tggattgctg gggtttgtgg
    18781 caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg gggaatttca
    18841 gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta ctctgcttct
    18901 cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact gtgctgggaa
    18961 ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag gcggggacgt
    19021 ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg ttaacgggga
    19081 tcattctggt tctggagatg accgataact accagctcat tttgccaatg attattaccg
    19141 gtcttggcgc aacactatta gcgcaattta ccggcgggaa acctctatac tcggcgattc
    19201 ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag gccgcatcag
    19261 ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta ttattgggtt
    19321 agaatttgcc cgattgccga tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc
    19381 cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct gacgaagata
    19441 acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc ttccagtatg
    19501 gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg
    19561 gcctggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt gactacaccg
    19621 aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt
    19681 gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta tccggcacat
    19741 cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg
    19801 aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg ctgtttccag
    19861 tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa tacaggcagt
    19921 tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg accagtacat cacgaatgat
    19981 cccgccgcca acgccagtaa tgacgcccat acaaaccgcg attaacggac cggcttccgc
    20041 attaaaggct ttattcacgc taatgccgac aaacaccgcc agaccaacgg cgtccagcac
    20101 cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg tcagcatgct
    20161 ggtgaccatg gcaacgacca gatcggtggg atctttcacc caaaataccg ggccgtgatc
    20221 cagcgccatg tcgcgaattg ttccgtcgcc tactgcggta accacgccca gtaccagaac
    20281 accaaaaggg tccatacgca attttccggc taacaaaacg ccgtagatgg caaataccgc
    20341 tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga gcacctgaaa
    20401 aggtcaggca ctaatctacc tgtgaaagcg cattacagag ctgttgtgct gcgaggataa
    20461 tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga attttgagct
    20521 gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta atgacaatag
    20581 cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga acccggctgt
    20641 ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc gactcttttc
    20701 cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta ggtttatcag
    20761 catattgcgc tttcaattgc acgtactgat ccagcaggga ttgcgcggct tgttcggcct
    20821 tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt tgttcaatgc
    20881 ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca acctgccgct
    20941 cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca atgcgttcca
    21001 gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga ggatagtcgg
    21061 aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca gtgttggcgg
    21121 gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcgaga aaagacaggg
    21181 cgaccagcgc cctgaacagt gatttagcca tgtgccagtt tctgcaccag tgactcaacc
    21241 atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag atgagactgt
    21301 tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa attgtggcag
    21361 acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa gttgtggcgg
    21421 attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat caggccacgt
    21481 acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca
    21541 gctttaaagc ctgccggaca gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca
    21601 tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat cgcccacttt cagcgttggt
    21661 gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg ttccaacagc
    21721 agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag cgcaacctcg
    21781 gttccattca gttggccggt atagatttcg caaccgccga gactgatagt ttgacggttt
    21841 tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat gccgattttc
    21901 atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc ttacggggaa
    21961 gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa aaataaactg
    22021 gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc tgcggatctt
    22081 cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac
    22141 ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc actcgatgga
    22201 agtccagcag gtgggacgct acatcgccaa agaaatttta agccgtctga aagagcttaa
    22261 attgctggaa gcatacggcc tggatgaact gaccggcccc tttgaaagca ttgttgagat
    22321 gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg aagcagcgat
    22381 aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc ctctgactga
    22441 cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc ttaacgagct
    22501 gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag gcattcgtct
    22561 ggtgcataca ttgatgcgga tgaatctcac ttgggcacag gttggcggta ttttaaaata
    22621 tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt taatgaaaaa
    22681 gccgggttat tatctttctg aagaagccta tattgcccgc ttgcgtaaag aacttaattt
    22741 ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg acatctccta
    22801 ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg agcagcttta
    22861 tcatcatttg cacgaagcgt ggggccagca tgagaaaggt tcgctctttt cgctggtggt
    22921 tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg aagatcagtt
    22981 ttttatgtat ttacgggtaa acaccctaaa taaactggta ccctacgcgg cacaacgatt
    23041 tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat tggaagatgc
    23101 cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac atgtgtttag
    23161 ccatccggat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg gattattaga
    23221 gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg tagaaaaaga
    23281 acgggtgaaa cgtttcccta ttgagtcgcg cttattccac aaactctcga cgcgccatcg
    23341 gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt ttccgctatg
    23401 ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga ccgacctcta
    23461 tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccagtct tttgtaaaga
    23521 cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcggg ctataaaacg
    23581 aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat acatgaaaaa
    23641 aaacacatta gcactgagtg cactggctct gagtttaggt ttggcgttat ctccgctctc
    23701 tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa gccttgcacc
    23761 gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta gcacaaccgt
    23821 taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt ctccgttctg
    23881 ccaggaaggt tctccgttcc agagctctcc gttctgccag gggggccagg gcggtaatgg
    23941 cggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg atgccgataa
    24001 aggctatgtc gtcaccaaca accacgttgt tgataacgcg acggtgatta aagtccagct
    24061 gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct ctgatatcgc
    24121 gctgatccaa atccagaacc cgaaaaaact gaccgcaatt aagatggcgg attctgatgc
    24181 actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggtctgg gcgagacggt
    24241 aacttccggg attgtctctg cgctggggcg tagcggcctg aatgctgaaa actacgaaaa
    24301 cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc tggttaacct
    24361 gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg gcaacatcgg
    24421 tatcggtttt gctatcccga gtaacatggt gaaaaacctg acctcgcaga tggtggaata
    24481 cggccaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt ccgaactggc
    24541 gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc tgcctaattc
    24601 ctccgctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga acggtaagcc
    24661 gatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg gcagcaaact
    24721 gaccctgggc ttactgcgcg acggtaagca ggttaacgtg aacctggaac tgcagcagag
    24781 cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggcg ctgagatgag
    24841 caacaaaggc aaagatcagg gcgtggtagt gaacaacgtg aagacgggca ctccggctgc
    24901 gcagatcggc ctgaagaaag gtgatgtgat tattggtgcg aaccagcagg cagtgaaaaa
    24961 catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac tgaacattca
    25021 gcgcggcgac agcaccatct acctgttaat gcagtaatct ccctcaaccc cttcctgaat
    25081 acgggaaggg gttctcctta caatctgtga acttcgccac aactccatac atcttcatca
    25141 tcctttgggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa gccgtgcata
    25201 acggaggact tatggctggc tggcatcttg ataccaaaat ggcgcaggat atcgtggcac
    25261 gtaccatgcg catcatcgat accaatatca acgtaatgga tgcccgcggg cgaattatcg
    25321 gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg gtactttcac
    25381 agggacgagt cgtcgatatc gatgacgcgg tagcacgtca tctgcacggt gtgcggcagg
    25441 ggattaatct accgttacgg ctggaaggtg aaattgtcgg cgtaattggc ctgacaggtg
    25501 aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa atgatgctgg
    25561 aacagtcgcg gttgatgcac ttgttggcgc aggatagccg tttgcgggaa gaactggtga
    25621 tgaacctgat tcaggcagag gagaatactc ccgcacttct gaatgggcgc aacggctggg
    25681 gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg gtcagcttgg
    25741 cgtggacagc gcaatggcgg agttacaaca actgcaaaac gcgctgacta cgcccgagcg
    25801 taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac cggcgttgaa
    25861 ctcttttggg cgctgggatg cagaagatca tcgtaagcga gttgaacaac tgattacccg
    25921 catgaaagag tacggccagc tgcgttttcg cgtttcactg ggcaactatt ttaccggtcc
    25981 tggcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg gtaaacagcg
    26041 gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt tactcgacag
    26101 tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc tgaaagcgat
    26161 ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg tttcgccaca atgtgcaacc
    26221 gctggcaacg tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc ggcttaatcg
    26281 tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc tgctgtatgt
    26341 ggcgttacaa ctggatgaag agcggtagat tatgcgttaa ggttgaggcg gcgctggctc
    26401 attcccctca gcccgaaggg gagaggggaa tgcccgagtt aaccgtcaat tcgggattat
    26461 ttacgcgtta gtttttccag atcggcttca atctcgctga tcttattggt tacgactgac
    26521 tccaggtgac gtaagtcgtc gaggatctta cgcttaagat caacttcgct gcggtcacgc
    26581 tgacagattt gatcaagctc atcaatgata taccgtagat tcgggctgat ttcctggact
    26641 tctttataac cctgacccac accatcagcg acgaccgttt tacgctgacg cggatattta
    26701 aacttaacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat tttcagaata
    26761 tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt ggtaataccc
    26821 agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa ttgtgcttat
    26881 tttagcattt ggcttggccc gcccggctcg actgtgatcg cagaaagcct ttttcttcca
    26941 gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtggcg atattccatg
    27001 ggatggatta gcacatttat cttcgttttg cgaattacat gacagtccga aacaagatga
    27061 aaaagtattt aaaaaagggg gaggaagaat gagtaggacg aattattctg agccagaaag
    27121 catacgggca gcatcatgct gcccgtatat gtactcttaa tcgatggtac gcagcagttc
    27181 gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa ccgcacagta
    27241 gaggctgtat ttgccatctt ttgacggcag attacctgaa acaaccacag accccgccgg
    27301 aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc tctgaccaat
    27361 gtatacgccc atagagatga cggaaccttc ttcgacaatc accccttcaa ccacttcaga
    27421 gcgtgcgcca atgaagcagt tatcttcaat aatggtcggg ttagcctgca gcggctccag
    27481 cacgccgccg atgcccacgc caccggaaag gtggacgttt ttaccaatct gcgcacaaga
    27541 accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc cgatgttaac
    27601 gtaagacggc atcagcacgg tgttacgggc aataaacgca ccctgacgta ccgccgctgg
    27661 tggcacaacg cggaagcctt ctttctggaa acgtgcttcg tcgtagtcgg cgaatttcat
    27721 cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat cattaatacg
    27781 gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac cgtcaatttt
    27841 ttccgctaca cgcagtgcgc cggaatccag cagggcgatc acctgattta ccgcttcgcg
    27901 ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg cgttcaaaag cggtttcaat
    27961 aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac atcacccttt
    28021 atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca actcgttatt
    28081 aagcgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct cgccaatggt
    28141 tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca aaaattttcc cgactcgcgc
    28201 cagcagtcca ggttggtcga gggcgatcag ttcgaggaac gatttgcggt cggtatgggt
    28261 cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt aatttggcgg gttggcgacg
    28321 gggctgcggt ggctgccagc tactttgcgt cagcacctgc tccagaccaa accgaataac
    28381 ctcatggcga tctgcggaca gcgggctgcc atcgggttcc agcacgataa aggtatccat
    28441 cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat tgcggcggtc
    28501 taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga taaaaatctc
    28561 ggtgcctcca cgcgtagcct gcgggctaag caataccagc ggtttgctta aatcatgctg
    28621 taataaatgg cgggcatgcc aggccagttg atttgggcta tggcggacaa aatagttagc
    28681 acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca tgcgcagtag
    28741 tgccagtgcc tggagttggt gatggcgaac ccgttcgcgc atatccggcg tgttttgcat
    28801 tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac tttgcttcca
    28861 gctattccac agtgtttcgt tggtggcgca aatgtcagcc actgtcaggc ataccagata
    28921 gcgcagacga ttttccgttt gaacttcttc ggcaaactgc ttgatgactt cagggtcctg
    28981 aatatcgcgg cgttgtgctg tcaccgacat cagcaagtgc tggcgaacca gccaggcaac
    29041 cagctgtgtt tcgcgtgagt tcagcccgtg gagttctgca aaatgcacta catcctgagc
    29101 accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca gcgcggcgat
    29161 gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg gatggcgctg
    29221 gcgcgtttct tcactggcaa aactctccag tttcagcatc acgcggatag tatgttcatc
    29281 caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatgcg accattgcgg
    29341 catatacgcg ccgagcacgc tatggcgatg cattggcaat agtccgcgcc gcaccgctcc
    29401 ggggtgacgc agaatgctca aaaacagttt tcgtgcttgc ggaatattac acagcggttg
    29461 ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg gtggagtaaa tgccggtgat
    29521 cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct ggcgcataaa
    29581 tagtgtttca tcacgcaggt cgattagcgt accgcgtagc tgaaactcat cgtcgagtgg
    29641 acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt gcagcagcat
    29701 ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca tccgctcgac
    29761 cggttcgttg ccttcaccac tgtaattcag acgctgagcg acgctaagtt ggcgatcgaa
    29821 taacagacga ttatcgtaac ggctgacgac cagatgcagg gcaaagcgaa tacgccacag
    29881 tatatgcaga cattcgttta attccgcccg ctccgctgag gttaagaagc caaacccgac
    29941 catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca gagtgtgaat
    30001 atcgcgcaag ccgccagggc tacttttgat gtctggttca aggttgtagc tggtgccatg
    30061 gtaacgctga tggcgctggt tctgttcttc aactttcgct gcgtagaact tgtcggaagg
    30121 ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aagagcgcaa catcgccaat
    30181 taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc cttccaacat
    30241 gcactcttca agcgtgcgca cactatgacc gacttccagc tttacatccc agagcagcgt
    30301 taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc ttaaaatcag
    30361 taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga cggcaaccaa
    30421 tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca ggagctggtc
    30481 tatgaactcg gtacgtgcct caatcaactg ttctgcagag atcccattgt caaaggcatc
    30541 acccagccaa cgctggaaag tatcgatatg ggcttttatc ccacagacgg ttaattcatc
    30601 acggggccag gcgcatggat tttgcggttg accgggcagg gtggagagag cggtgtttgc
    30661 gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta tcgcattaaa
    30721 aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc gggatggtgt
    30781 catccttgcg tagcgtcaaa atttcgcagc cgttatcagt caccacaata gtatgctcat
    30841 attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc atggtgcgga
    30901 tctctttttt acccgcgttg accattggct caatggtgaa cgtcatcccc ggtttcagca
    30961 cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg aagccgcgac
    31021 caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg acaaatttct
    31081 gaatcgccgc accgatttcg cgcagattaa tgcctggttt taccatgcgt agcgccaggt
    31141 acaggctttc ttgcgtgatg cggcacaggc gttcgcccat gatggtcggc ttaccgacga
    31201 taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca tcaatgttaa
    31261 cgatatcgcc atctttcagc agcttagcat cgtccgggat accgtggcac accacttcat
    31321 taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca gaaaccgcgt
    31381 gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg ctgacgcccg
    31441 gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca gcgacgcgca
    31501 ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc catcagcgtc
    31561 ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg gcaaattgag
    31621 aatcattctg aatttcgcca aacgtgccac tgaaggtttt ctataataga aaattcgacg
    31681 tctgatgctg tacacagcgc caacaattat tggtgtccac gacgtatttg tggtataaag
    31741 cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac aatctcactt
    31801 tgtgtaacaa cacacacgta tcggcacata ttccggggtg ccctttgggg tcggtaatat
    31861 gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg gcaactgttt
    31921 ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt tactggaacc
    31981 cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc aaccttgaga
    32041 aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgtt tctcgcaaag
    32101 gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa gacgctgctc
    32161 tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg actaactgga
    32221 aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct caggacggta
    32281 ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg gagaaactgg
    32341 aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg tttgtaatcg
    32401 atgctgacca cgaacacatt gctatcaaag aagcaaacaa cttgggtatt ccggtatttg
    32461 ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg ggtaacgacg
    32521 acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta cgtgaaggcc
    32581 gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag taataaggct
    32641 tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag aattcccggg
    32701 agcttacatc agtaagtgac cgggatgagc gagcgaagac aacgcatctg cggcgcgaaa
    32761 tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg ggcctgcata
    32821 tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc acatctccga
    32881 ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg agcgtactgg
    32941 cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca tcgagctggc
    33001 aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag gcaacgttgc
    33061 tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc tggaagttaa
    33121 ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag acaaagttct
    33181 ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac agttcgaaga
    33241 agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc gcgttgctgc
    33301 gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg ttctggttgc
    33361 tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg ctgcaagcaa
    33421 gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag aataccaggt
    33481 acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga aaatggttga
    33541 aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt tcgttatgga
    33601 accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga ctggcttcat
    33661 ccgtttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag cagaagttgc
    33721 tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg cttctttttg
    33781 tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg acgtacaatg
    33841 tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat ggctaccaat
    33901 gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct gcagggcact
    33961 gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat caaagaactg
    34021 gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt ccgtggcgct
    34081 ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat gctggcgacc
    34141 gtaatgaacg gcttggcaat gcgtgatgca ctgcaccgcg cctatgtgaa cgctcgtctg
    34201 atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga agctatcagc
    34261 ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc gttctttacc
    34321 accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt gctgaaagca
    34381 accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc aaccatgtac
    34441 gagcaactga cttacagcga agtgctggaa aaagagctga aagtcatgga cctggcggcc
    34501 ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaatatgaa caaaccgggt
    34561 gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga ataattcccg
    34621 tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgtaag tctggttata
    34681 aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg agcgtacaaa
    34741 agtacgtgag gatggcgagc actgctcggg gccaaaatgg caaataaaat agcctaataa
    34801 tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact tccactgtgt
    34861 gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat tagcgatatc
    34921 agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac ccaaatcagc
    34981 aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt ggaatattac
    35041 ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc ccgtactctg
    35101 aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat tatggcctcc
    35161 gatctcggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct gccgccgctg
    35221 acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga acaagcgcgt
    35281 gttgcagtac gtaatgtgcg tcgtgacgcg aacgacaaag tgaaagcact gttgaaagat
    35341 aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa actgactgat
    35401 gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact gatgcagttc
    35461 tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc tggcggcgtt
    35521 ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca ttctgggctc
    35581 gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg aacacttccg
    35641 cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt gcctggaatt
    35701 ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta aaacgatgct
    35761 acagcaacag gggagccgca ccgaagtctt aagtgggcaa caagccgctt gcgatatggc
    35821 agcgcttgag gaggttgatc aggtgatggc agccattgtt ggtgctgctg ggctgttacc
    35881 tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag aatcactggt
    35941 tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat tgttaccggt
    36001 cgatagcgaa cataacgcta tttttcagag tttaccgcaa cctatccagc ataatctggg
    36061 atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt ctggtggccc
    36121 tttccgtgag acgccattgc gcgatttggc aacaatgacg cccgatcaag cttgccgtca
    36181 tccgaactgg tcgatgggac gtaaaatttc tgtcgattcg gctaccatga tgaacaaagg
    36241 tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga tggaagtgct
    36301 gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca gtgttctggc
    36361 gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat ggccgaatcg
    36421 cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga catttgccgc
    36481 accggattat gagcgttatc catgcctgaa actggcgatg gaggcgttcg aacaaggcca
    36541 ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt ttcttgcgca
    36601 acaaatccgc tttacggata tcgctgcgtt gaatttatcc gtactggaaa aaatggatat
    36661 gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcga gtgaagtcgc
    36721 cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag agtcgcgcta
    36781 tttgttagcg tagggcttca gtgatatagt ctgcgccatc tgatcgtaag tagttggctt
    36841 tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct tcagtattcc
    36901 tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc tactcaacca
    36961 cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat ggacggcaat
    37021 ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc cggggcaaaa
    37081 tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaggcgtt aacgctgtat
    37141 gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt aatggaactg
    37201 tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt gcgtctgcgt
    37261 attattggcg ataccagtcg ttttaactcg cgtttgcaag aacgtattcg taaatctgaa
    37321 gcgctaacag ccggaaatac cggtctgacg ctgaatattg cggcgaacta cggtggacgt
    37381 tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg aaacctgcaa
    37441 ccagatcaga tagatgaaga gatgttaaac cagcatgtct gtatgcatga actggcccct
    37501 gtagatttag taattaggac tgggggagag catcgcatta gtaacttttt gctttggcaa
    37561 attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga tgaacaagac
    37621 tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg caccgagccc
    37681 ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat atctgctttt
    37741 gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg gttcgccatt
    37801 gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag cggttttacc
    37861 actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc gctgatgctt
    37921 tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat ctcactttgg
    37981 gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc aggttccgca
    38041 gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac cattgttccc
    38101 ttcttctggg gcatgctggc gttacgggcc tggcactatg acgagaatca ttacagtggc
    38161 gcaatatggc tgctctatgt catgatcctg gtatggggcg ctgactccgg cgcatatatg
    38221 tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg taaaacctgg
    38281 caaggcttta taggtggact cgctactgca gcggtaatct catggggtta tggcatgtgg
    38341 gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc agcgttagcc
    38401 tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat taaggacagc
    38461 ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct gacggctgcg
    38521 gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg gaaggtaata
    38581 tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta cttatcaccg
    38641 tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt gagcgtttct
    38701 caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc gaatatgtta
    38761 tcgccctgat cccgttgggc ggttatgtca aaatgctgga tgagcgcgca gaaccggtcg
    38821 ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga gcggcgatta
    38881 ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg ctggttttta
    38941 ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat agcagccaat tcgatagctg
    39001 cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc gaaacgcctg
    39061 attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc accaccatta
    39121 cagtagcgcc atttgacagc gaccaacggc gggatgtaaa gctcgattta cgtcactggg
    39181 cgtttgagcc tgataaagaa gatccggtat cttcgctggg gattcgtcct cgtgggccgc
    39241 aaattgaacc tgtactggaa aatgtgcagc caaactcggc ggcaagcaag gcaggtttgc
    39301 aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg gtgacctttg
    39361 tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa aggcagggga
    39421 gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa gcgattggtt
    39481 ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt gtacgccagt
    39541 atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg atgaagctga
    39601 cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac ctcagtgggc
    39661 cgatctctat cgccaagggg gctgggatga cagcggaact cggggtagtt tattacctgc
    39721 cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg ttgcccgtac
    39781 ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga ccggtatccg
    39841 agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg ttaatggggc
    39901 ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac gcataataac
    39961 gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca ccgtatacgg
    40021 tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg tcgccgttgg
    40081 tgcggccctc ctcagtatgc cggtgcgtac aggcgacacg gttaatgatg aagatatcag
    40141 taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg tccttcgtga
    40201 tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca ttactttctc
    40261 cggtaacaaa tcggtgaaag atgacatgct gaagcaaaac ctcgaggctt ctggtgtgcg
    40321 tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc tggaagactt
    40381 ctactacagc gtcggtaaat atagcgccag cgttaaagct gtcgtgaccc cgctgccgcg
    40441 caaccgtgtt gacctaaaac tggtgttcca ggaaggtgtg tcagctgaaa tccagcaaat
    40501 taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt tccaactgcg
    40561 tgacgaagtg ccgtggtgga atgtggtagg cgatcgtaaa taccagaaac agaaactggc
    40621 gggcgatctt gaaaccctgc gcagctacta tctggaccgc ggttatgccc gtttcaacat
    40681 cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca cggtgaacat
    40741 caccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc ttgccgggca
    40801 ctccgctgaa attgagcaac tgactaagat cgagccgggc gagctgtata acggcactaa
    40861 agtgaccaaa atggaagatg acattaaaaa gcttctcggt cgctatggtt atgcctatcc
    40921 gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat tacgcgtgaa
    40981 cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta acgatacctc
    41041 gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggt tggggagcga
    41101 tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa ctgtcgatac
    41161 cgatacccaa cgtgttccgg gtagcccgga ccaggttgat gtagtctaca aggtgaaaga
    41221 acgtaacact ggtagcttca acttcgggat tggttacggt actgaaagtg gcgtgagctt
    41281 ccaggctggc gtgcaacagg ataactggtt aggtacaggt tatgctgttg gtatcaacgg
    41341 gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt acttcaccgt
    41401 agatggcgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag atgacgccga
    41461 cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct tcccgattaa
    41521 cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt ccaacatgca
    41581 gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga gcacctctga
    41641 tcaggataac agcttcaaaa cggacgactt cacgttcaac tatggttgga cctataacaa
    41701 gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg gtaaagtgac
    41761 cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga cttatgtgcc
    41821 gatcgatgac gatcacaaat gggttgttct gggtcgtacc cgctggggtt atggtgatgg
    41881 tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt ccagcaccgt
    41941 gcgtggcttc cagtccaata ccattggtcc gaaagcagtt tacttcccgc atcaggccag
    42001 taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga aagacctgtg
    42061 taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg agttcatcac
    42121 cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcat tcttctggga
    42181 tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat atccggacta
    42241 tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga tgtccccatt
    42301 ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag acaaggcaga
    42361 acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg aatgtagtgg
    42421 tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca aagaactgca
    42481 ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta ttagctgcag
    42541 gtcttggttt agcactggca acttctgctc aggcggctga caaaattgca atcgtcaata
    42601 tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg ctggaaaatg
    42661 agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag gctaaaatga
    42721 aaaaactgca gtccatgaaa gcgggcagcg atcgcaccaa actggaaaaa gacgtgatgg
    42781 ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc gcacgtcgtt
    42841 ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa tccgttgcca
    42901 acagccagga tatcgatctg gttgttgatg caaacgccgt tgcttacaac agcagcgatg
    42961 taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct tcaattcgac
    43021 tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat atcgtcatca
    43081 ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg gttaacccaa
    43141 aataccgtga gcatttaggc ttgtgccagg cgtccgcggt tgtcatgacc caggacgatc
    43201 ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact tacgcgcgca
    43261 tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc agtgcggtga
    43321 tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg gtgattgagt
    43381 ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta ggtaaaaaca
    43441 gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat gagatccaga
    43501 tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc tttggttatg
    43561 ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt attggcgatc
    43621 gcgtggagat cggtgcctgc acaaccatcg atcgcggcgc gctggatgac actgttattg
    43681 gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg attggcgaca
    43741 atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt cgttactgca
    43801 tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa gtgacggtta
    43861 cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc tcaggcattc
    43921 cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac attgatgaca
    43981 tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa cgttccatct
    44041 tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc cgtgttatta
    44101 ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc atactctgca
    44161 gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg tggatcgcgt
    44221 gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg tcaatgagcc
    44281 attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga ttctggaagc
    44341 aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg aaccgggtga
    44401 gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg tgcctggcga
    44461 tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc gttttaaagg
    44521 ggttgctctg gtcgatggta aagtagtttg cgaagcaacg atgatgtgtg ctcgtagccg
    44581 ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca ttgtggaaga
    44641 gggcgcgtca attggcgcga acgcacacat tggtcctttt tgtatcgttg gaccccatgt
    44701 cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc atactaaaat
    44761 tggccgcgat aatgagattt atcagtttgc ctccatcggc gaagttaacc aggatttgaa
    44821 atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt aaccgcattc gcgaaagcgt
    44881 caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca gcgacaactt
    44941 actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct gtattctcgc
    45001 caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca tcggcggcat
    45061 gaccgcagtc catcagttct gcatcattgg tgcgcatgtg atggttggcg gctgctccgg
    45121 tgtggcgcag gacgtccctc cttatgttat tgcgcagggt aaccacgcaa cgccgttcgg
    45181 tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta ccgctatccg
    45241 caatgcgtat aagctaattt atcgtagcgg taaaacgctc gatgaagtga aaccggaaat
    45301 tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct ttgcacgctc
    45361 aacgcgcggt ctgattcgtt aatgactgac cagcgtccat taacgattgc cctggtcgcc
    45421 ggagaaacct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa agaacgtgtg
    45481 cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg ctgcgaagcc
    45541 tggtacgaaa tggaagaact ggcggtgatg ggcattgttg aagtgctcgg tcgtctgcgt
    45601 cgcttactgc atattcgtgc cgatctgaca aagcgttttg gcgaactgaa gccagatgtt
    45661 tttgttggta ttgatgcgcc cgacttcaat attactcttg aaggtaacct caaaaagcag
    45721 ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg cgtggcgaca gaaacgcgtt
    45781 ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga aaaagcgttt
    45841 tatgacaaat acaatgtacc gtgccgcttt atcggtcata ccatggctga tgccatgccg
    45901 ttagatccag ataaaaatgg tgcccgtgat gtgctgggga tcccttacga tgcccactgt
    45961 ctggcattgt tgccgggcag ccgtggtgca gaagtcgaaa tgcttagtgc cgatttcttg
    46021 aaaactgccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt gccgctggtg
    46081 aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagtcgcgcc agacctgtcg
    46141 gttcatttgc tggatggaat gggccgtgag gcgatggtcg ccagcgatgc ggcactactg
    46201 gcgtcgggga cggcagccct ggagtgtatg ctggcgaaat gcccgatggt ggtgggatat
    46261 cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga ttatgtctcg
    46321 ctgccaaatc tgctggcggg cagagagtta gtcaaagagt tattgcagga agagtgtgag
    46381 ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga acgggaaaac cagccacgcg
    46441 atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga tgagcaggcg
    46501 gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac acgcagctgg
    46561 ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc accgctgcgg
    46621 tgatccttga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag ctgagcgaaa
    46681 aacgccgtct ggcgctctat gaagagatca aagagaaagc gttgagctgg agtctgggcc
    46741 gcgcggaacc ccacgaaatc gacgagctga acattcttca tgcgaccatg ctggcgatgc
    46801 agcgtgccgt cgctgggctg catattgcgc cggaatatgt gttgattgat ggtaaccgct
    46861 gcccgaaatt accgatgcct gcgatggctg tggtgaaagg ggatagccgc gtaccagaaa
    46921 tcagtgccgc gtctatcctg gcgaaagtga cgcgtgacgc cgaaatggcg gcgctggata
    46981 ttgttttccc gcaatatggt tttgcccaac ataaagggta tccaaccgct tttcatctgg
    47041 aaaaactggc tgaatatggc gcgactgaac atcatcgtcg cagctttggg cctgtcaaac
    47101 gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg aatctgaaga
    47161 tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg atcgatggct
    47221 tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca gcactggcga
    47281 tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga catggcgcag
    47341 ggattaagcc tatcgtcggg gcagatttta atgtccagtg cgacctgctg ggtgatgagt
    47401 taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg gcgttgctga
    47461 tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat cgcgactggc
    47521 ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg gcgcatgggc gacgtcggac
    47581 gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt tatgaagaac
    47641 acttcccgga tcgctatttt ctcgagctga tccgcaccgg caggccggat gaagaaagct
    47701 atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg gcgactaacg
    47761 atgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc gcgatccacg
    47821 acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag caatatatgc
    47881 gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc agaagccctt gccaacaccg
    47941 ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg tgaatacttc ctgccgcagt
    48001 tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa gaaggcctgg
    48061 aagagcgtct ggcattttta ttccctgacg aggaagaacg tcttaaacgc cgcccggaat
    48121 atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggcttc ccgggctact
    48181 tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg gtagggccag
    48241 gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc gacctcgatc
    48301 cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc tccatgcctg
    48361 acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgaacac gtggcggaca
    48421 tgtacggtcg tgatgcggta tcgcagatta tcaccttcgg tacgatggcg gcgaaagcgg
    48481 tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgac cgtatctcga
    48541 aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc gagccgcagc
    48601 tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg gcgcgcaaac
    48661 tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc gcgccgacca
    48721 aaattaccga ttttgcgccg ctttattgcg atgaagaggg caaacatccg gtcacccagt
    48781 ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt ggtttgcgta
    48841 cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg aagaatggcg
    48901 agccaccgct ggatatcgcc gcgatcccgc tggatgacaa gaaaagcttc gacatgctgc
    48961 aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag gacctgatca
    49021 agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg ttccgccctg
    49081 gtccgttgca gtcagggatg gtggataact ttatcgaccg taaacatggt cgtgaagaga
    49141 tctcctatcc ggacgtgcag tggcagcatg aaagcctgaa atcggtactg gagccaacct
    49201 acggcattat cttgtatcag gaacaggtca tgcagattgc ccaggtgctt tctggttata
    49261 ccctcggtgg cgcggatatg ctgcgccgtg cgatgggtaa gaaaaagccg gaagagatgg
    49321 ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac gccgaactgg
    49381 cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta cggatttaac aaatcgcact
    49441 ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgcac tatccggcgg
    49501 agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg gtgggcctgg
    49561 tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac tccggtcttt
    49621 accatttcca cgtcaacgac gatggcgaaa tcgtgtatgg tattggcgcc atcaaagggg
    49681 taggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc tacttccgcg
    49741 aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt aaaccggcgg gtgctggaaa
    49801 aactgatcat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggcg ctgatgaact
    49861 cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct atcggtcagg
    49921 ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc tacgccagct
    49981 gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta ggcctgtacc
    50041 tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc ggaggcgtaa
    50101 ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacggctgcg gggctcgttg
    50161 ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc acgctggatg
    50221 accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa taccagcaat
    50281 tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat gacttcagcg
    50341 gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg gaaaaatatg
    50401 ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt ttaaaccgac
    50461 tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc tactatcaga
    50521 gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct ccgagcgatc
    50581 gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg gagtttgact
    50641 aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt gcagagctgg
    50701 aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg gatattaaca
    50761 tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt aaaatcttcg
    50821 ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccgcagcgt ccttataccc
    50881 tggattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac cgtgcgtatg
    50941 cagacgataa agctatcgtc ggtggtatcg cccgtctcga tggtcgtccg gtgatgatca
    51001 ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt ggtatgccag
    51061 cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc tttaagatgc
    51121 ctatcatcac ctttatcgac accccggggg cttatcctgg cgtgggcgca gaagagcgtg
    51181 gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgccttggc gtaccggtag
    51241 tttgtacggt tatcggtgaa ggtggttctg gcggtgcgct ggcgattggc gtgggcgata
    51301 aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt tgtgcgtcca
    51361 ttctgtggaa gagcgccgac aaagcgccgc tggcggctga agcgatgggt atcatcgctc
    51421 cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc ggaaccgctg ggcggcgctc
    51481 accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg gatctggccg
    51541 atctcgatgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc ctgatgagct
    51601 acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc ccttttttat
    51661 cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac acatgaacat
    51721 cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca aagaactgga
    51781 gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg ttgatttgct
    51841 gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg atgagtacag
    51901 tctcgattta tgcagcgata tcaatcaact taatgaatat ctcccgcttt atgccttcat
    51961 caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc tctggttttt
    52021 tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt acaccaacga
    52081 atatcttgat aacattacgc cgccgttcac gaaagccttg tttacctacg tcaaagagcg
    52141 gaagtacacc ttttgtacgc cggggcatat gggcggcacc gcatatcaaa aaagcccggt
    52201 tggctgtctg ttttatgatt ttttcggcgg gaataccctt aaggctgatg tctctatttc
    52261 ggtcaccgag cttggttcgt tgcttgacca caccgggcca cacctggaag cggaagagta
    52321 catcgcgcgg acttttggcg cggaacagag ttatatcgtt accaacggaa tatcgacgtc
    52381 gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga tcgaccgcaa
    52441 ctgccataaa tcactggcgc atctgttgat gatgaacgat gtagtgccag tctggctgaa
    52501 accgacgcgt aatgcgttgg ggattctggg ggggatcccg cgccgtgaat ttactcgcga
    52561 cagcatcgaa gagaaagtcg ctgccactac gcaagcacaa tggccggttc atgcggtgat
    52621 caccaactcc acctatgatg gtttgctcta caacaccgac tggatcaaac agacgctgga
    52681 tgtcccgtcg atccacttcg attccgcctg ggtgccgtat actcattttc atccaatcta
    52741 ccagggtaaa agtggtatga gcggcgagcg tgttgcggga aaagttttct tcgaaacgca
    52801 atcgacccac aaaatgctgg cggcgttatc gcaggcttca ctgatccaca ttaaaggcga
    52861 gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct cgcccagtta
    52921 tcccattgtt gcttcggtta agacggcggc ggcgatgctg cgtggtaatc cgggcaaacg
    52981 gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc agcggctgcg
    53041 ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg atgaagccga
    53101 atgcaggccc gttgcgcctg gcgaacaatg gcacggcttt aacgatgcgg atgccgatca
    53161 tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg aacagggcaa
    53221 tatgagtgag gaggggatcc cggcggcgct ggtagcaaaa ttcctcgacg aacgtgggat
    53281 cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg gcatcgataa
    53341 aaccaaagca atgggattat tgcgtgggtt gacggaattt aagcgctctt acgatctcaa
    53401 cctgcggatc aaaaatatgc tgcccgatct ctatgctgaa gatcccgatt tctaccgcaa
    53461 tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac acgatctttc
    53521 cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc cacatcaggc
    53581 atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac tggtcggtag
    53641 agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga tgccaggaga
    53701 aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt gttccgtagg
    53761 gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg aagacggcgt
    53821 ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt ccgggcgagt
    53881 aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt tcaccatatt
    53941 gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat tcttggtttc
    54001 acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga tttggcgctt
    54061 aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg ccccagccga
    54121 ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga tgcggcagtg
    54181 gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc atacacgcaa
    54241 aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta tgagcagtaa
    54301 ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt aaaatcgcac
    54361 catcatgaca ctcacgctca atagacaact tctcacctca cgccagattc tggtggcctt
    54421 tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc ggacggaaaa
    54481 tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca atgccgatgc
    54541 ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg tggtcgaacg
    54601 cgtacaactt gcgcaagagg ggctggggat tgaggcccag gcgcggcagg cacgttatca
    54661 ggcatttgcc cgtaccctat tgcccggcga agtgctggtc accgcgcaac atctcgacga
    54721 tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc ggccctgccg ggctttcggc
    54781 tatggcggaa gtttcggagt ttgctggaac acggcttatc cgcccgttgc ttgcccgcac
    54841 gcggggggaa ctggagcagt gggcattggc gcatggttta cgctggattg aagacgaaag
    54901 taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc cgttattgca
    54961 gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc gccacacttt gtgctgaaca
    55021 agagagtctg ttggatgaac tgctggcgga tgatttagca cactgccaaa cgtcgcaggg
    55081 gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgtcgcgcgg cgattattcg
    55141 ccgctggctg gcagggcaga atgcaccgat gccttcccgc gacgcgctgg tgcgcatctg
    55201 gcaggaagtg gcgctggcgc gggaagatgc ttccccctgt ttacgtttgg gcgcctttga
    55261 aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc aaagcgaaac
    55321 cattgtgctg tggcagacgt ggctacaacc gctggaatta ccggcggggc tgggaaccgt
    55381 acagcttact gcgggaggcg atattcgccc tccgtgtgca gacgaagcgg tcagcgtgcg
    55441 tttcaaagcg ccaggattgc tgcatattgt cgggcgcaac ggcggacgta agctgaagaa
    55501 aatctggcaa gagttaggcg tgccgccgtg gctacgtgac accacgccac tgctgtttta
    55561 tggagaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg tggctgaagg
    55621 tgagaatggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag ccggataaga
    55681 cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc gcttaccacc accgtaccga
    55741 tttcagggtg gctaaagctg gtaattttat ccagacgcag ctcgcgggtt tcgccagcgg
    55801 cctcgacgac cagatactcc acatttttgc gggagactaa atcactggct tttgcctgca
    55861 atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag gcgagctcaa
    55921 gattatcgta atcatcacaa ttaattggtt gatacgtatc attcattgac ataatcgctc
    55981 accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga aagggcgtcg
    56041 tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg aacgtatcct
    56101 aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata tttttccaca
    56161 tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta cgaagataaa
    56221 ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc cgcaactgca
    56281 cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg cttaaagcga
    56341 caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt ctcgatgcct
    56401 ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg cgcttgctgg
    56461 cgtggctgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt gccgatgatg
    56521 agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt gagctggggc
    56581 tgccggatga gcgacgaatt aagaaagcct gcacccaggc cgcagaagtg gcgctgttta
    56641 cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa tgtgtgcagt
    56701 ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta agcgcctttg
    56761 ccgatcgtac catgacgctg caggcaacga ttcaggatgg tgttatctgg ttatcggatg
    56821 ataagaataa tctggaagtt aacttaaccg cctggcaaca accttcatga ttgtgatttc
    56881 ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg cgcagggcgc
    56941 tggcgggcag catgttaata agacctcaac ggctattcat ctgcgttttg acattcgggc
    57001 gtccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc atttgatcag
    57061 cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac tgaaccgcga
    57121 agccgctctg gcccggctgg tggcagtgat taaagattta acaaaagaac aaaaagcccg
    57181 acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga aagcacaaaa
    57241 atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt cgggaataaa aagaaggaat
    57301 ggatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt actctgatgg
    57361 gatgtaataa tcgggccgaa gtcgatacgc tttctccggc gcaggctgcc gaactgaaac
    57421 cgatgccgca aagttggcgc ggcgtgctac cgtgtgccga ttgcgaagga atcgaaatct
    57481 ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc ggtgctcgtg
    57541 aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac aagctggtat
    57601 taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg ctggagatgc
    57661 tcgatcgtga aggcaatcca attgaatcgc agttcaacta tacgctggaa ccggcacaat
    57721 ccagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg gctgatgcgg
    57781 cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac gcagagctgg
    57841 agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg tcagtagaag
    57901 gtcactttac gcttgaggct aatccggata ctggtgcgcc aaccaaagta ttagcgcccg
    57961 atacggcggg taaattttac ccgaataagg attgcagtag tttggggcag taaacctgct
    58021 taaggcagaa acaaacgcaa aattgcctga tgcgctgcgc ttatcaggcc tacagcatat
    58081 aatgcaacat tttgaattag cacgattttg taggccggat aaggcgttta cgccgcatcc
    58141 ggcattttgt gcacgaggct ggctttatca ctgagtcagc ccaacaaagc gacccgcctt
    58201 gatataaatc ccaggcttca ggtgcccaac atactgtaac tgcgtttctg gcttaaacgc
    58261 agagacatca ccaatacgca tatgcatcag atcggcaggg ctaatccagc ccgattgcgc
    58321 cagtgtaagt ggatgcccgg atttggcaaa tgcatcagtg acaaattccg aacaaaacca
    58381 cgactttttg tctccttcgc acacactgct taactgcgct ttcgccaggt cgctgacgca
    58441 ctgttggcga aaatcctcgg agaacggatt cagtgagcac atctggcgag tcaccataaa
    58501 gggaataaat tcgacaatgc cgcgatagtt ataaccgcta tctttgattt tattggcaaa
    58561 cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga cgaaaagctt
    58621 atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc ctgtcgcttc
    58681 tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag tgctgaagac
    58741 gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc cgggtttgag
    58801 atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat gctggaattt
    58861 gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg gctggctgat
    58921 atcaacggta caagccgaaa gtaaaagaaa gctggggaga agcaggcggc agtacgcctt
    58981 tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag gaccctttct
    59041 ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc ttaattaact
    59101 gtttctcgcc gttgcgacga tatttatatt cgatatcgtc gttgtcgagg ttacggtcgc
    59161 ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc acgcccggac
    59221 gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg ctgtacagtt
    59281 tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga atcgccacct
    59341 ggaacggcgc gatagcgtca ggccatacga tgccgcgttc gtcgtagttc tgctcaatcg
    59401 ccgcagctac cacacgcgtt accccgatac cgtagcaacc catcgtcagg atttggttac
    59461 ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta cccagctgga
    59521 agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctggcca tccgggcttg
    59581 gatcgccagc caccacgtta cggatatctg caacttccgg ggtagcgaca tcgcgatccc
    59641 agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa tcactcatcg
    59701 ccgcaacggt acggtcaatc accaccggaa tcggcatgtt taccggaccc agtgaacccg
    59761 gaccggcttt aaccacagca cgaatttctt cttcggtcgc gaaagtcagc gggcttgcaa
    59821 cctgcggcag tttttctgct ttaacttcgt tcagctcgtg atcaccgcgc accagcagcg
    59881 caaccagcgg gaagctactg ccttcaaccg ctttaaccag cagagtctta accgttttct
    59941 caatcggcag attgaactgt tcaaccagtt ccgcgatggt tttcgcgttc ggcgtatcaa
    60001 ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt cggcgcgata gcttctgcca
    60061 gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct tcaccgctct
    60121 gcgccagcac ctggaattcg tgagaggcgc tgccgccgat agaaccggtg tcggcctgta
    60181 cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca tacattgcat
    60241 cgtaggtttc ctgcagggat tcctgagaag tatggaaaga gtaagcatct ttcatcagga
    60301 attcgcggga acgcatgacg ccgaaacgcg gacgcacttc gtcgcggaac ttggtctgga
    60361 tctgatagaa gttcagcggc agctgtttgt aagagctaag ctcgttacga atcaggtcag
    60421 tgataacttc ttcatgagtt gggccgagta cgaacggacg ctcgccacgg tcaacaaaac
    60481 gcagcagttc cggaccgtac tgttcccaac gaccactctc ttgccacaaa tcggctggct
    60541 gaaccaccgg catcgacacc tcgatcgcac cggcgttgtt catctcttca cgcacgatgt
    60601 tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtatataac ccggaggcca
    60661 gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg gcgtcggcag
    60721 gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta cggttccagt
    60781 tggaaggtag aacaggctca aggcgagcct gggacaaaaa aagtgattta gtttaccagt
    60841 gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg caaagacttc
    60901 aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga cggcataggt
    60961 tttgcccgtt tcctcacctt tacgataggc cgggcgcggg tcctgcgcca gtacttcgcg
    61021 gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct tttcgacctc
    61081 tgcggtaaaa ctcaccgcca tctctgcagc tggcgcactt tgcgcatagc tggcactggc
    61141 atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg gcgtaccatc
    61201 gaccagatcc agactgccga gcttcagaat cacgctgcct ttatggcaaa caacctcttt
    61261 cagctctacc agcgacatgc caattgggtt agggcggaaa gtagagcgtg ttgcgaaaac
    61321 ccccattctg gcgttaccgc cgaggcgcgg tggacgcaca gtcggacgcc agccgccttc
    61381 catcgtttga tgaaagacga aaaggatcca taaatggctg aatgcttcca ggccgcgaac
    61441 ggcgtcggcc tggttgtagg aagctatgag atgcagttcg ccgttggcgc tttttaccag
    61501 acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa cgcctatttg
    61561 ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat acatacagcc
    61621 tgacgatagc agcccggcgt accgctggtg acttcgcagc tatgcagtaa tacagcattg
    61681 gctttcattt tagaggcatt gatttgcatc tgcttacgtg cggttggaat gctcggcgga
    61741 gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc gcggaacggt
    61801 ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg cggcgctttc
    61861 ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc gacaggggat
    61921 ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg taaagcacgc
    61981 atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct tgtaaaaatg
    62041 acaagacggg caagcgcccg tcctgaatga tattacaaat tgtggaaaca gcctaaaaat
    62101 taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta aacttcatca
    62161 gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc ttcacgcgtt
    62221 acgatcaggt ttacgtatgg ggagtcttta tcttcgacaa agataccgtc tttcgccgga
    62281 gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat ttgcgcgtcg
    62341 tccagagaac gcggcagttg cggtgcttcc agttcaacaa ttttcagatt ttttgggttc
    62401 tcaacaacat caagaacggt cggcagcagg ccaacgccat ctttcagttt gatcaagccc
    62461 actttttgca gcagcagcag tgaacgacca agattagttg gatcgtttgg cacggcaacc
    62521 tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc agcaatcgga
    62581 taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag ttgctgatca
    62641 aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa tgcttcgttt
    62701 ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt gtctttcgca
    62761 actttctgcg cgacttctgc tacctgctgt tcggcaccaa caatcacgcc gactttaatg
    62821 tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc aatcagggct
    62881 cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt caattattta
    62941 tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc tgcaaactga
    63001 attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt cgcgttgtag
    63061 ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac tgcaccaccc
    63121 atcgcggaat aaccgaccag ggtaatcagg gtgatagttg ccgcattcac cagacccggc
    63181 aacgcttccg gtaacagcac tttacagacg atctgcattg gcgtggcacc cattgcgcgg
    63241 gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat acgggcaata
    63301 aacggtgctg caccaacggt taacggaaca attgctgcct gcaaaccaat cgatgtaccg
    63361 acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg gatggaacgg
    63421 aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat aatttgcccc
    63481 ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaaccc aaaaaatccg
    63541 gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca catcatcggc
    63601 tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg caatggcggc
    63661 ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaatt taacgccacc
    63721 ggcgtagtcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac gcgcggtttc
    63781 agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcagca tcggcacgca
    63841 gtcagtaaat ggctccgttt gcagacgttc ctggtaatct tccgggatat ccagatgcag
    63901 ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca cttcacttac
    63961 cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac aaatacgctt
    64021 cacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc ggttaatgtc
    64081 tttcagcagt tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg tggcttcatc
    64141 acacagcaat actttgggat tgctggctaa tgcacgggca atcgccacac gttgtttctg
    64201 cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa ccaatgacag
    64261 caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct ccagcggcag
    64321 agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga aaatcatacc
    64381 aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcaatt cctggccatc
    64441 gaccagcacg ctaccctcgg ttgggcgctc cagcaagttt acacaacgta taagcgtact
    64501 cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca catgcaggct
    64561 gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg tgatattcga
    64621 aagttttatc attgattatt tattatcgtc attaagttag tcgtagcatc tcgaatgcct
    64681 gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct aaatcaatca
    64741 ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg tcccatgcct
    64801 tattaaggag ctataaaagg tggcgaagag cgtacccgca atttttcttg accgtgatgg
    64861 caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat ttatcgacgg
    64921 tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg tagtaaccaa
    64981 ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc tgaccgagtg
    65041 gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt attgcccgca
    65101 tcatccgcag gggagtgttg aagagtttcg ccaggtctgc gattgccgca aaccacatcc
    65161 ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt cttatatggt
    65221 gggcgataaa ttagaagata tgcaggcagc ggttgcggcg aacgtgggaa caaaagtgct
    65281 ggtgcgtacg ggtaaaccta ttacacctga agcagaaaac gcggcagatt gggtgttaaa
    65341 tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcgc aatgattaaa
    65401 agatgaacgg ttgaaataaa aatgcatttt tccgcttgtc ttcctgagcc gactccctat
    65461 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    65521 aagagaaaaa atcctgaaat tcagggttga ctctgaaata ggaaagtgtg atatacgcca
    65581 cctcgcgaca gtgagctgaa agctgcgtcg caactgctct ttaacaattt atcagacaat
    65641 ctgtgtgggc actcgaagat acggattctt aacgtcgcaa gacga
//
LOCUS       DAJTRH010000010        65558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTRH010000010 DAJTRH010000000
VERSION     DAJTRH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO339_000716"
     CDS             complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO339_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5925455.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1152..1238)
                     /locus_tag="OO339_000717"
     CDS             complement(1152..1238)
                     /locus_tag="OO339_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5925456.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO339_000718"
     CDS             complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO339_000718"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5925457.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO339_000719"
     CDS             complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO339_000719"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5925458.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3587..3631
                     /locus_tag="OO339_000720"
     CDS             3587..3631
                     /locus_tag="OO339_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925459.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO339_000721"
     CDS             complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO339_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5925460.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="OO339_000722"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="OO339_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5925461.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="OO339_000723"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="OO339_000723"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5925462.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="OO339_000724"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="OO339_000724"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5925463.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="OO339_000725"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="OO339_000725"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5925464.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="OO339_000726"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="OO339_000726"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5925465.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..11476)
                     /gene="setA"
                     /locus_tag="OO339_000727"
     CDS             complement(10298..11476)
                     /gene="setA"
                     /locus_tag="OO339_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5925466.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="OO339_000728"
     CDS             complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="OO339_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5925467.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11798..13453
                     /gene="sgrR"
                     /locus_tag="OO339_000729"
     CDS             11798..13453
                     /gene="sgrR"
                     /locus_tag="OO339_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5925468.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13487..13586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13617..14600
                     /gene="thiB"
                     /locus_tag="OO339_000730"
     CDS             13617..14600
                     /gene="thiB"
                     /locus_tag="OO339_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5925469.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14576..16186
                     /gene="thiP"
                     /locus_tag="OO339_000731"
     CDS             14576..16186
                     /gene="thiP"
                     /locus_tag="OO339_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5925470.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16170..16868
                     /gene="thiQ"
                     /locus_tag="OO339_000732"
     CDS             16170..16868
                     /gene="thiQ"
                     /locus_tag="OO339_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5925471.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16982..17746)
                     /locus_tag="OO339_000733"
     CDS             complement(16982..17746)
                     /locus_tag="OO339_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5925472.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17832..18710)
                     /gene="araC"
                     /locus_tag="OO339_000734"
     CDS             complement(17832..18710)
                     /gene="araC"
                     /locus_tag="OO339_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5925473.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19049..20749
                     /gene="araB"
                     /locus_tag="OO339_000735"
     CDS             19049..20749
                     /gene="araB"
                     /locus_tag="OO339_000735"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5925474.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20760..22262
                     /gene="araA"
                     /locus_tag="OO339_000736"
     CDS             20760..22262
                     /gene="araA"
                     /locus_tag="OO339_000736"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5925475.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22462..23157
                     /gene="araD"
                     /locus_tag="OO339_000737"
     CDS             22462..23157
                     /gene="araD"
                     /locus_tag="OO339_000737"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5925476.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23232..25583
                     /gene="polB"
                     /locus_tag="OO339_000738"
     CDS             23232..25583
                     /gene="polB"
                     /locus_tag="OO339_000738"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5925477.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25748..28654
                     /gene="rapA"
                     /locus_tag="OO339_000739"
     CDS             25748..28654
                     /gene="rapA"
                     /locus_tag="OO339_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5925478.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28666..29325
                     /gene="rluA"
                     /locus_tag="OO339_000740"
     CDS             28666..29325
                     /gene="rluA"
                     /locus_tag="OO339_000740"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5925479.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="OO339_000741"
     CDS             complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="OO339_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5925480.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30512..32866
                     /gene="lptD"
                     /locus_tag="OO339_000742"
     CDS             30512..32866
                     /gene="lptD"
                     /locus_tag="OO339_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5925481.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32919..34205
                     /gene="surA"
                     /locus_tag="OO339_000743"
     CDS             32919..34205
                     /gene="surA"
                     /locus_tag="OO339_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5925482.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34205..35194
                     /gene="pdxA"
                     /locus_tag="OO339_000744"
     CDS             34205..35194
                     /gene="pdxA"
                     /locus_tag="OO339_000744"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5925483.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35191..36012
                     /gene="rsmA"
                     /locus_tag="OO339_000745"
     CDS             35191..36012
                     /gene="rsmA"
                     /locus_tag="OO339_000745"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5925484.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36015..36392
                     /gene="apaG"
                     /locus_tag="OO339_000746"
     CDS             36015..36392
                     /gene="apaG"
                     /locus_tag="OO339_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5925485.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36399..37241
                     /gene="apaH"
                     /locus_tag="OO339_000747"
     CDS             36399..37241
                     /gene="apaH"
                     /locus_tag="OO339_000747"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5925486.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37319..37798)
                     /gene="folA"
                     /locus_tag="OO339_000748"
     CDS             complement(37319..37798)
                     /gene="folA"
                     /locus_tag="OO339_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5925487.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="OO339_000749"
     CDS             complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="OO339_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5925488.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="OO339_000750"
     CDS             complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="OO339_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5925489.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40422..41816)
                     /locus_tag="OO339_000751"
     CDS             complement(40422..41816)
                     /locus_tag="OO339_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5925490.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="OO339_000752"
     CDS             complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="OO339_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5925491.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42159..43445)
                     /locus_tag="OO339_000753"
     CDS             complement(42159..43445)
                     /locus_tag="OO339_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5925492.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43496..44437)
                     /locus_tag="OO339_000754"
                     /pseudo
     CDS             complement(43496..44437)
                     /locus_tag="OO339_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44452..45222)
                     /locus_tag="OO339_000755"
     CDS             complement(44452..45222)
                     /locus_tag="OO339_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5925493.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45578..47092
                     /gene="caiT"
                     /locus_tag="OO339_000756"
     CDS             45578..47092
                     /gene="caiT"
                     /locus_tag="OO339_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5925494.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47123..48265
                     /gene="caiA"
                     /locus_tag="OO339_000757"
     CDS             47123..48265
                     /gene="caiA"
                     /locus_tag="OO339_000757"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5925495.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48394..49611
                     /gene="caiB"
                     /locus_tag="OO339_000758"
     CDS             48394..49611
                     /gene="caiB"
                     /locus_tag="OO339_000758"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5925496.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49685..51238
                     /gene="caiC"
                     /locus_tag="OO339_000759"
     CDS             49685..51238
                     /gene="caiC"
                     /locus_tag="OO339_000759"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5925497.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51347..52132
                     /gene="caiD"
                     /locus_tag="OO339_000760"
     CDS             51347..52132
                     /gene="caiD"
                     /locus_tag="OO339_000760"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5925498.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52138..52728
                     /gene="caiE"
                     /locus_tag="OO339_000761"
     CDS             52138..52728
                     /gene="caiE"
                     /locus_tag="OO339_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5925499.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52814..53209)
                     /gene="caiF"
                     /locus_tag="OO339_000762"
     CDS             complement(52814..53209)
                     /gene="caiF"
                     /locus_tag="OO339_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5925500.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53471..56692)
                     /gene="carB"
                     /locus_tag="OO339_000763"
     CDS             complement(53471..56692)
                     /gene="carB"
                     /locus_tag="OO339_000763"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5925501.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56710..57858)
                     /gene="carA"
                     /locus_tag="OO339_000764"
     CDS             complement(56710..57858)
                     /gene="carA"
                     /locus_tag="OO339_000764"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5925502.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58314..59135)
                     /gene="dapB"
                     /locus_tag="OO339_000765"
     CDS             complement(58314..59135)
                     /gene="dapB"
                     /locus_tag="OO339_000765"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5925503.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRTALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59302..60216)
                     /gene="rihC"
                     /locus_tag="OO339_000766"
     CDS             complement(59302..60216)
                     /gene="rihC"
                     /locus_tag="OO339_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5925504.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60282..61232)
                     /gene="ispH"
                     /locus_tag="OO339_000767"
                     /gene_synonym="lytB"
     CDS             complement(60282..61232)
                     /gene="ispH"
                     /locus_tag="OO339_000767"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5925505.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61234..61683)
                     /gene="fkpB"
                     /locus_tag="OO339_000768"
     CDS             complement(61234..61683)
                     /gene="fkpB"
                     /locus_tag="OO339_000768"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5925506.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61770..62264)
                     /gene="lspA"
                     /locus_tag="OO339_000769"
     CDS             complement(61770..62264)
                     /gene="lspA"
                     /locus_tag="OO339_000769"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5925507.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62264..65080)
                     /gene="ileS"
                     /locus_tag="OO339_000770"
     CDS             complement(62264..65080)
                     /gene="ileS"
                     /locus_tag="OO339_000770"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5925508.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65123..>65558)
                     /gene="ribF"
                     /locus_tag="OO339_000771"
     CDS             complement(65123..>65558)
                     /gene="ribF"
                     /locus_tag="OO339_000771"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR5925509.1"
                     /translation="RQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVP
                     LRRQVSPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRH
                     IQVVLRKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
BASE COUNT        15828 a        17876 c        16937 g        14917 t
ORIGIN      
        1 gatgtccagt cccgtactct acgcgccaga gggaaattca cctggcgcgt atttttgttc
       61 gcggattagc tacggctgag cacgccgcgg cgatagagat tacgtttaat gcgcgttaaa
      121 ccaggttttg gcttacgcgg ttcgtccagg cttgccagga caatctccag cacacgctct
      181 gcgacatcgc ggtgacgttg agccactgcc agcaccggac actgtaagaa gtcgagcagt
      241 tcgttatcgc caaaggtggc aattgccagg tcagaaggca gtttgccgtc gcgacgcagc
      301 gtgacatcca tcactccttg caacaacgca aacgacgttg tgaacagcgc ctgcggcatc
      361 ggatgcgttt ccagccattt ttcgaataac tgggcagccg cctcccgctc atagctgttg
      421 gcatacagga aatgcacttc gcgcggatca tctttccaag cagtacggaa accttgttca
      481 cgcaggaagc tgacagaaag ctccggcagc gcgccaagat aaagcaccgt ctcggcggga
      541 aacttacgta actcttccgc cagcatttcg gcatcatcct gatcggcacc aaccacgctg
      601 gtgaaatgtt cacgatcgag ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc
      661 cagcgttgat aaaaaggatg ctcaggtggc aacgacgtcg aaacaataat ggcatcaacc
      721 tgacgctgta aaaggtgctc aatgcagcgc atttcgttgt ctggctgatc ttctgagcag
      781 gcaatcagca gttgataacc ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg
      841 gtatagctgg tgttctccag atcggggatc acaagaccaa tagaacgtgt gcgtccagca
      901 cgaagcccag ctgccacggc gttcgggtgg taattgtgct cacgcaccac agccatgact
      961 ttttcaacgg ttttgtcgct cacacggtat tgcttcgctt tgccgttaat aacatagctt
     1021 gcagtggtcc gcgacactcc cgccagccga gcgatttcat ccagtttcac aattgcccct
     1081 tgcgtaaaat gtaaaaacca tagccattgt actggtatgg gttaaattca ttaacatcta
     1141 agcgcagaaa attaactgcg gcaactgctt ttattccagg ttcccactga tttcgcaaaa
     1201 aaagcccaac gtgacatgtt gggctgcaaa ttgcgcactg agatcattct caacgcatta
     1261 ttttatcgcc gcgcgaaagt ccgaccacac cagagcgagc aacttccaca attttcgcca
     1321 catcgcgaat cgatgctaaa aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg
     1381 tataaagcga gggggtgaca tcgataattt gcccacggaa tatttccgta ttacgtttca
     1441 cttcgtcacg cccgtaaccg ctggcctgaa ttttcaccag catgatttcc cgctcaacat
     1501 gcgcgccctg ccccaactca ctcacgcgca agacatcgac cagcttgtgt aattgctttt
     1561 cgatctgctc aagtactttt tcatcgccca cggtctggat ggtcatacgc gataatgtcg
     1621 gatcgtcggt tggcgcaacg gtcaggcttt caatgttgta gcctcgctgg gaaaaaaggc
     1681 caatcacgcg ggataacgcg cctgattcat tttcgagtaa gactgataat atccggcgca
     1741 taatcaggtt ctctccgttt tgcttaacca catttcatcc attccgcccc cgcgaatctg
     1801 catcgggtag acgtgctcac tgccatcgac ggtaacatca acaaacacca ggcgattatt
     1861 gcgcacctgt tccagcgcct cgctaagttt gctttccagc tcatgcggat gagagatctg
     1921 gatcccgacg tgaccatagg cttccgccag acggacgaaa tcgggtagtg attgcatata
     1981 agattgtgaa tgacggccgg aatagatcat gtcctgccac tgtttcacca tccccagata
     2041 gcggttattg agattcacca ccagtacggg caactcgtat tgcaacgcgg tagacagttc
     2101 ctggatgttc atctgaatac tgccgtcgcc agtgacgcaa accacggttt cttctggcaa
     2161 cgccattttg acgcccagtg ccgcaggtaa accaaaaccc atcgtgccga gaccgccgga
     2221 attgatccag cgacgcggtt tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg
     2281 cccgacatcg gacgtcacgt aagcgtcccc attagtcaac cgccaaagag tctcgatcac
     2341 cgcctgcggt ttaatctttt cactgtgagt gtcatatttc aggcactgaa gagcgcgcca
     2401 ctgttcaatc tgctgccacc agtcgcggat ctcatccagc ggttgatggg cggattcttg
     2461 cgacaggagt tcaagcattt gttcgaggac ctggcgagca tcccccacaa tcgggatatc
     2521 cgcagtcacg gttttagaaa tggaagtagg atcaatatcg atatgcagaa cagtggcatt
     2581 tgggcagtac tttgccagat tgttcgtcgt tcggtcatca aatcgtaccc cgacggcgaa
     2641 aatcacatcc gcgttatgca tcgtcatatt ggcttcgtag gtaccgtgca ttcccagcat
     2701 gcccagtgcc tgacgatgcg ttgccggaaa cgcccccatt cccatcaatg aggaaacaac
     2761 gggcagattc aacgcctcca ccgtttcttt caactgctga tggcagcccg ccatgattgc
     2821 cccaccgcct acgtagacaa ccggtttttt tgccgctacc agcgtttgca gagcacgctt
     2881 aatttgccct ttatgtccgg tagtagtggg attgtaagaa cgcatactga ccgactcagg
     2941 gcagacatag ggtaatttgt tcgccggatt aagaatatct ttcggtaaat caacgaccac
     3001 tggtcctggg cgaccacttg ccgccagcca gaaagccttt ttcagcacct gcggaatgtc
     3061 ttccgtttgc ttaaccagaa aactgtgttt aaccaccggt cgcgaaatcc ccaccatgtc
     3121 gcactcctga aaggcatcgt aacctatcaa cgaggtcgct acctgcccgg aaaggacaac
     3181 taatggaatg gaatccatat aagcggtggc gatgccggta atcgcattgg tcgcccttgg
     3241 acccgacgtt accagcacga cgccgacttc cccggtcgca cgcgccaggc catcggccat
     3301 atgcaccgcc gcctgctcat gacgaactaa tacatgatca ataccaccca cggtatgcaa
     3361 tgcatcgtaa atatcaagga ctgcgcctcc gggataaccg aatacttgtt taacgccctg
     3421 atcgataagc gatcggacga ccatctcggc tccagacaac atctccatgg cctgcctcac
     3481 tgtttgacgg aaaaaatgtg taaaacacat taaccgtccg atcttgctta agcaattggc
     3541 agccagagac ataaacgcag ggtaataaga ataaacagga ggaaaggtga aaagaaacga
     3601 taaaatcctc cattacgaag gattaaattg ataataaatc ggctgaatcc cacaacttac
     3661 agcaatttct ctattttttt aattaatcct aaaatagaga gtccacacat tcagaataaa
     3721 attctacatt caataaaaaa tgcagaataa accagacatt catgtctggc ctattctgca
     3781 ttcagttagc gtttgcaaat tgagactaat tgttcttcca tccactgatg gcctttatcg
     3841 cgcccggcag cttcatgcca agagagataa caggttctgc tgttttgttt taacggcagc
     3901 ggtaatacct gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata
     3961 gcgaccaaat gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac
     4021 gcgatactgg cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga
     4081 tcgagcgaaa ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccttta
     4141 attgttggat gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg
     4201 gtaaattcag gacgatggaa gtcttcataa ctaatcacaa actccgtttc ctgataacgc
     4261 agctgatgtt cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca
     4321 atctgctcaa tgtgattata aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca
     4381 caaagatgaa atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt
     4441 tgtactagtt gcaatgcctg gcgaactgaa ccaaaaagtt gaaatgcgcg agcagtcggt
     4501 tgaataccac ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca
     4561 acagcgttac tgaccgcagg ttgcgacata cccagaacat gagcggcacg ggtaatgttt
     4621 tgctcctgca tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt
     4681 ggcttgctta actccgccgt ctctgaatga tctgtttgta cctctggcat acttaactcc
     4741 actgtcacac ttaactccct ttcccttatt ggaatgcaga gaaataatcc tgaagaatat
     4801 gatttatcat gcatataaaa taagaaaaag caaaatgagt aaaattcgga aaacataaag
     4861 atacgggttc tgataaaaac agaatccatt gccgtggaat gagtcattta ccacagcata
     4921 ataatccata atgttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattaat ttatatattt aatgcattaa
     5041 ttcttaacat taattgttca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tagacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccaag
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcac caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaaactcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc cagtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccgctctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttctg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact ctcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgctatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
    10501 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
    10561 ccatacggct atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa
    10621 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
    10681 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
    10741 aatcgctact gatccacaat ggcatatcaa taatgtacat agtgttgcag gtccacatta
    10801 acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata
    10861 aagcattttc cgacggcagt tctacccgcg ccacagacag aagcataaat gcaatcaata
    10921 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca
    10981 acgccagcat aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca
    11041 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag
    11101 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
    11161 gcgtcagata atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc
    11221 aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc
    11281 ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg
    11341 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
    11401 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg
    11461 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
    11521 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
    11581 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
    11641 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
    11701 ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
    11761 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
    11821 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
    11881 actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca
    11941 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac
    12001 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
    12061 tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt
    12121 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
    12181 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
    12241 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
    12301 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
    12361 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
    12421 caatacgctg ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct
    12481 ggatatccat ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc
    12541 gatgatcctg ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac
    12601 cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga
    12661 cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc
    12721 cgacgagcca gccggtggtc tgatgctaaa aggtccacag ggcgagaaaa aagagattga
    12781 aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc
    12841 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
    12901 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
    12961 ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt
    13021 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
    13081 tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat
    13141 cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt
    13201 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
    13261 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
    13321 cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat
    13381 gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
    13441 accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
    13501 gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
    13561 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
    13621 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
    13681 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
    13741 aagcttttga agccaactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
    13801 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
    13861 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
    13921 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
    13981 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga
    14041 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca
    14101 cgccggggct gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag
    14161 cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg
    14221 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
    14281 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
    14341 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
    14401 tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt
    14461 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca
    14521 cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
    14581 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
    14641 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
    14701 ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
    14761 ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta
    14821 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
    14881 cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc
    14941 atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat
    15001 tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact
    15061 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    15121 ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct
    15181 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca
    15241 ggcaaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg
    15301 cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca
    15361 gcgactaagt aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga
    15421 cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt
    15481 gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct
    15541 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    15601 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    15661 gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    15721 ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca
    15781 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    15841 agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct
    15901 gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact
    15961 ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt
    16021 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    16081 tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    16141 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    16201 atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc
    16261 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc
    16321 cagccagcgg ttcgctgact atcgatggtg ttgatcacac cacgacgccg ccgtcacgcc
    16381 gtccggtgtc gatgctgttt caggagaaca acctgttcag ccatctgacg gtcgcgcaga
    16441 acatcgggct ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc
    16501 acgcgattgc ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt
    16561 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    16621 tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc
    16681 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    16741 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc
    16801 agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta
    16861 cgggttagta tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg
    16921 ttttgtaggc ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc
    16981 cctaaacccc aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa
    17041 ttaacaccac cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac
    17101 ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc
    17161 agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact
    17221 cgccgctctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga
    17281 agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg
    17341 gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac
    17401 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg
    17461 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    17521 agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga
    17581 aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta
    17641 gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg
    17701 aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    17761 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    17821 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
    17881 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
    17941 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    18001 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
    18061 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
    18121 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga
    18181 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    18241 tcaagcagat tcatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    18301 atttgcccaa acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc
    18361 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
    18421 taaacccact ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct
    18481 cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc
    18541 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    18601 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    18661 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    18721 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    18781 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    18841 taacaaagca ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    18901 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    18961 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    19021 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    19081 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    19141 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    19201 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    19261 gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac
    19321 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    19381 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    19441 tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta
    19501 ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaggaca gcgccgtggc
    19561 gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac
    19621 cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca
    19681 cgaaagctgg ggcggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa
    19741 tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac
    19801 cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg
    19861 cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa
    19921 agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg
    19981 ggcagttaaa ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat ttatcggtct
    20041 ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg
    20101 gccgctggaa cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca
    20161 gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct
    20221 gccggtggtg ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa
    20281 aggggtgatt accgatctta acctcgctac cgacactccg ctgctgttcg gcggtttgat
    20341 tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc
    20401 cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc
    20461 ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct
    20521 cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca
    20581 gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg
    20641 ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct
    20701 tccaacttcc gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa
    20761 tggcgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc
    20821 cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg
    20881 aagcgaaact gccctacaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca
    20941 ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc
    21001 acaccttctc cccggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc
    21061 tgcaattcca cacccagttc aacgcggcgc tgccgtggga cagtatcgat atggacttta
    21121 tgaacctgaa ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc
    21181 gtcagcaaca tgccgtcgtt accggtcact ggcaggataa acaagcccat gagcgtatcg
    21241 gctcctggat gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgat
    21301 ttggcgataa catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca
    21361 agttcggttt ctccgtcaat acctgggcgg ttggctatct ggtgcaggtg gtgaactcca
    21421 tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc
    21481 ctgccacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc
    21541 tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag
    21601 atttgcacgg tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt
    21661 acggctttgc gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga
    21721 tgtcaaccgg tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga
    21781 aaggtaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg attgccgtag
    21841 aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc
    21901 gactgatctt caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg
    21961 atcgttaccg tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga
    22021 aactgccggt ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact gcttccgaag
    22081 cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg
    22141 atatgcgcca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc
    22201 gcctgccagc gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct
    22261 aagtagccac atcaggtatg taacgcctga tgcgacgctg acgcgtctta tctggcctac
    22321 acgctgcgat tttgtaggtc ggataagcaa agcgcatccg gcattcaacg cctgatgcga
    22381 cgctggcgcg tcttatcagg cctacgcgct gcgattttgt agaccggata agcgaagcgc
    22441 atccggcacg aaggagtcaa catgttaaaa gatctcaaac gccaggtatt agaagccaac
    22501 ctggcgctgc caaaacacaa cctggtcacg ctcacatggg gcaacgtcag cgccgttgat
    22561 cgcgagcgcg gcgtctttgt gatcaaacct tccggcgtcg attacagcgt catgaccgct
    22621 gacgatatgg tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc
    22681 tcctccgaca cgccaactca ccggctgctc tatcaggcat tcccctccat tggcggcatt
    22741 gtgcatacgc actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca
    22801 accggcacca cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc
    22861 gacgcagaaa tcaacggcga atatgagtgg gaaaccggta acgtcatcgt agaaaccttt
    22921 gaaaaacagg gtatcgatgc agcgcaaatg cccggcgttc tggtccattc ccacggcccg
    22981 tttgcatggg gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc
    23041 gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg
    23101 ctgctggata aacactatct gcgtaagcat ggcgcgaagg catattacgg gcagtaatga
    23161 ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg gttttttgat
    23221 ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc
    23281 aagggacaga agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg
    23341 caccgcaaga gtccgtggcg tttattcacg ccgatcaggt tccccgcgct cagcatattt
    23401 tgcagggtga acaaggcttt cgtctgacac cgctggcgtt aaaggatttt caccgccagc
    23461 cggtgtatgg cctttactgt cgcgcccatc gccaattgat gaattacgaa aagcgtctgc
    23521 gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg
    23581 agcggtttat cacctcaccg gtgtgggtcg agggtgatat gcacaatggc actatcgtta
    23641 atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctatagata
    23701 ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggctgc gggcagcgca
    23761 tcgtttatat gctggggccg gagaatggcg acgcctcctc gcttgatttc gaactggaat
    23821 acgtcgccag ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca aaccacgatc
    23881 ccgatgtgat catcggctgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg
    23941 ccgagcgtta ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg
    24001 agcacggctt taaaaacggc gtcttttttg cccaggctaa aggtcggcta attatcgacg
    24061 gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg
    24121 ctcaggagct attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa
    24181 ttgaccgccg tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg
    24241 agctggtgac gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcaa
    24301 cggtgaacgg cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct
    24361 attttccgcg aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc
    24421 acgccagccc tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg
    24481 tgctggacta taaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg
    24541 ggctggttga aggcatggcg caacctgatc cagagcacag taccgaaggt tttctcgatg
    24601 cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc
    24661 gcgataaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga
    24721 atgcctttta tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat
    24781 cgtcgatcac catgcgtggt catcagatca tgcggcagac caaagcattg attgaagcgc
    24841 agggctacga cattatctac ggagataccg actcgacttt tgtctggcta aaaggtgcac
    24901 attcggaaga agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt
    24961 gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaactc
    25021 atttctgccg ttttcttatg ccaaccattc gcggagctga taccggcagt aaaaagcgct
    25081 acgccgggct gattcaggag ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg
    25141 tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct
    25201 tccgcaacga gccatatcag gagtacgtcc gccaaaccat cgacaaactg atggcgggtg
    25261 aactggatgc gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc
    25321 gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg
    25381 gtcgcccctt gcaatatcag aatcgcggca ccattaagta cgtatggacc accaacggcc
    25441 cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc
    25501 tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct acacttatga
    25561 ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg
    25621 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    25681 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    25741 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    25801 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    25861 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    25921 tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    25981 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    26041 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    26101 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    26161 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    26221 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    26281 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    26341 cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    26401 aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc
    26461 tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt
    26521 tgcccgtcgt agcaaacagc gcttggaaca tctctgtgaa gccgaatggg acctgctggt
    26581 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    26641 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    26701 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    26761 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    26821 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    26881 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    26941 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    27001 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    27061 gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    27121 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    27181 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    27241 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    27301 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    27361 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    27421 ggtactgctg tgctcagaaa tcggttctga aggacgtaac tttcagttcg ccagtcacat
    27481 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    27541 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    27601 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    27661 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga
    27721 tcaaaccgaa ggctttgacg atctgatcaa aagctgccgc gagcaacatg aagcgctgaa
    27781 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    27841 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    27901 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    27961 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    28021 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    28081 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag
    28141 cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    28201 gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    28261 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    28321 tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    28381 cgccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga tcgagaaatc
    28441 tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct
    28501 gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    28561 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga
    28621 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    28681 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg
    28741 gtcaacaagc cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc
    28801 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    28861 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    28921 cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc
    28981 cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    29041 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    29101 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt
    29161 gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    29221 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    29281 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    29341 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    29401 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    29461 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    29521 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    29581 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    29641 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    29701 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    29761 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    29821 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    29881 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    29941 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    30001 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    30061 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    30121 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    30181 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    30241 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    30301 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    30361 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    30421 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    30481 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    30541 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    30601 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    30661 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    30721 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    30781 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    30841 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    30901 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    30961 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    31021 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    31081 ccgcgaagaa caagttgctg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    31141 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    31201 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    31261 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    31321 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    31381 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    31441 ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt tcaacgtcga
    31501 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    31561 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    31621 caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc
    31681 agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat
    31741 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    31801 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    31861 agcgaagttt ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    31921 cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa
    31981 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    32041 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    32101 gtctctgctg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    32161 ccgtattgcc tccgctaacc aagtgacgac cggtgtcaca tctcgcatat atgatgatgc
    32221 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    32281 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    32341 cgacacctac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    32401 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    32461 tctggtacag ctgaattacc attacgccag cccggaatat attcaggcta cgctgcctaa
    32521 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    32581 caggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    32641 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    32701 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    32761 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    32821 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    32881 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    32941 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    33001 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    33061 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    33121 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    33181 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    33241 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacactt
    33301 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    33361 gtcgcatcac catcctgcca caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    33421 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    33481 cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    33541 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    33601 gcggccagat ggggtggggc cgtattcagg agttgccggg gatcttcgcc caggcattaa
    33661 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    33721 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    33781 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgc gtgaaactgg
    33841 agcaaattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    33901 tctcgcagga tccaggttct gctaaccagg gcggtgatct cggctgggct acaccagata
    33961 ttttcgatcc agccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    34021 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    34081 aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    34141 aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca
    34201 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    34261 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    34321 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    34381 ttcccccaac tcccctgcac aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc
    34441 gctacgtgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    34501 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    34561 ccccgtgcat aaaggcgtca ttaacgacgc aagcattccg tttaccggtc ataccgagtt
    34621 tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    34681 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    34741 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    34801 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    34861 tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa
    34921 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    34981 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    35041 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    35101 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    35161 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    35221 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    35281 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    35341 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    35401 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    35461 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    35521 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    35581 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    35641 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    35701 atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    35761 gtcgtgcgcc tggtccctca tgcaacgatg cctcacccgg ttaaagatat tcgcgtgttg
    35821 agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    35881 aacctgttta gcgtcgaggt gttaaccgga atggggatcg acccggcgat gcgagcggaa
    35941 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    36001 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    36061 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    36121 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    36181 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    36241 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    36301 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    36361 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    36421 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccccg
    36481 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg
    36541 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    36601 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    36661 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc
    36721 tgcaaatcga cgaagagaaa aagctggtga tggcccacgc agggatcacg ccgcagtggg
    36781 atctgcagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct
    36841 atcccttctt tcttgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    36901 gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt ttttgcttcc
    36961 cgaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct gccccactga
    37021 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    37081 gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat accggctgct
    37141 gctggggtgg gtcattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    37201 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatacaggaa
    37261 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaactt
    37321 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga
    37381 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    37441 ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttggcagg aactgctcat
    37501 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcaatggctt
    37561 catccaccga tttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    37621 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    37681 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    37741 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    37801 agatttcccg ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg
    37861 tgcgaggatg atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg
    37921 gactcgccgc attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc
    37981 actctttatt aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta
    38041 ccttcttctg ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg
    38101 gtgatgatct cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg
    38161 tcattctcaa ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg
    38221 gcacgttctc gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt
    38281 ttcagcgcac cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga
    38341 atgtagtgat cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc
    38401 accatctccg tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc
    38461 gctttcgccg ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc ataaaacact
    38521 ttcataccaa atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt
    38581 ttcaccccgc tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg
    38641 ataatcacgc gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa
    38701 gattgctcaa ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc
    38761 accgccaggg tcagcgattt cgcccactcc ggctccagca cattcgccat ctgcgccgca
    38821 ccaaatacca caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc
    38881 tgtttatttg gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg
    38941 aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct
    39001 atcgacatgc caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata
    39061 tcgctttcca acgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg
    39121 cccatcgcca tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat
    39181 aacgccacgg cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga
    39241 cgcgtaacat agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc
    39301 gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc
    39361 gccaccagcg ggattgccgc gatatcctgg aacagcagca ctgcaaaggc actgcggccc
    39421 atttgcgtca ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgttgatgag
    39481 agcgccagcg tcataccgat caattccgcg acctgccagc gcagcccaag taacatacag
    39541 aacagcccca gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc
    39601 gcacgcagct tccacagcct ttgtggatcg agttcgaggc cgataataaa cagcatcagc
    39661 accaccccaa tctcggcaaa gtgcagaata gattcggcat cggtcaccag tcgcagcccc
    39721 cacgggccaa taatgcagcc ggcgatcagg tagccaagta ccgatcccag accaagacgt
    39781 accgcaatgg gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta
    39841 tgactatcca tgatgggcct cctgccattc cagcagacgt tgcttatagt ggcgcgcctg
    39901 cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca
    39961 gttcagcccg cagtagattg ccgtcgcctg tagcggctgc gacagcacat caaagcccgg
    40021 atgcgcacca atttcaaaat ggctttcccc gccgccggtc gtcaccgccc acagcaaatg
    40081 tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca aaactttatc
    40141 gatccaaagt ttaagaagcg gaggaatgct gtaccactgc atcgggtgct gccagacgat
    40201 cagatcggcg cgagagagcg cctcctgctc ggcggcaata tcgatattga agtcaggata
    40261 gagttgataa agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt caagcatccg
    40321 tttattcgca tgggaatgat gcggatacgg atgcgcataa atgatgagga tcatagttat
    40381 tagcccgcgt gtttacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt
    40441 atagtcaata atattgattc agaaaagaga gaaatctgaa caaataatcc cattctctgc
    40501 ccgcggatcg tcatattgct ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac
    40561 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    40621 ataccgtaat tattgataaa tatcggcagt gcccaggtcg aaacaatggt gccaatacga
    40681 cttaaggaca taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagttcatta
    40741 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    40801 atcactacca gccagatccc catatccggg attagcccca aaaccgccaa cgccagcgtc
    40861 atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttag ccacagcatc
    40921 ggcggaatac agccgagcat aaagaacagg ctaatcacca cattccctag tgccgcgttt
    40981 ttgccaaccc ccaatcccaa cgaaccaacg atttgcgggc caaaggtgta aatggcgaac
    41041 attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg
    41101 cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt
    41161 tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac
    41221 cagcggggag attctggcag ttcgaatcga ccaatcaaaa tcaacaaaca agggatcgcc
    41281 gcgctaccca gcatccagcg ccaaccacct tccacatcat aaagccagta gccgaccaga
    41341 tcggcgcagg tcgcgccgac ataccacatc gcggcaataa agctgatgga aaacgcccgc
    41401 tgacgggtac tggagaactc ggtgatcatc gaagtggcga tgggataatc tgcaccgatg
    41461 acaatgccga tgagtacccg catcaccaac agttcgacgg gggatgaaac acacatcgtc
    41521 gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact
    41581 ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg
    41641 cccagcaagc caatccagtc agcgtccagt ttcagcgccg gagtcagttg ctccagcgcc
    41701 acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa
    41761 atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt
    41821 tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc
    41881 gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc
    41941 cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact
    42001 gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcac
    42061 ctgtttatca gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt
    42121 attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc
    42181 atgccatcct tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg
    42241 cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc
    42301 agttccgggt tgccgctaaa catgcgtggg ttatcaagga acgccggtag tttctggtac
    42361 atgcgcatat cgcgcagcgg gccactctcc agatgctgac gatattccgc gagtttttgc
    42421 ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg
    42481 gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca
    42541 tcaccggcaa tcaatacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc
    42601 ggcactacgt gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgcaacggcc
    42661 ggatgctgtt tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc atgcagatga
    42721 tgcagaccac aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg
    42781 cccatcaggc catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc
    42841 aactgaaaac ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc
    42901 gccacatccg tcggtttaac gcgttttgcc atccccaact tttcggcaag gatggagttc
    42961 accccatcag caaggagcac cgttttcgct tcaatcacat caccatcggc ttctacaccg
    43021 acgactttgc catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg
    43081 cccgcttctt cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa
    43141 gaacgctggg atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt
    43201 tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggaatcggcg
    43261 aaacctggaa taatgtgttc cagactgtgg gcatagagac gcccgccagt aacgttcttg
    43321 gcacctgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc
    43381 agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg
    43441 gacataagag ctccctgtca gcggcagagc cgtaatgcag ccctgccaga gtggatcaac
    43501 gcgctaaagc tgcggtcagt gccggaagga tcttcacggc atcgccaaca atgccgtaat
    43561 ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgacg
    43621 cgttagcgcc aaccatgtgc tggatctacc cggagatccc caccgccagg tacagttcag
    43681 gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt
    43741 ctgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca
    43801 gcgcaatgtt ctctttgctg ccaatgccgc gaccgacgct gaccaccaga cgggctttgt
    43861 cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg
    43921 gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat
    43981 cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa
    44041 tcgccagacc gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt acgctgacgg
    44101 tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt
    44161 tgccgcgacg ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg cgaatagtgt
    44221 cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc
    44281 agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg
    44341 cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaggggg
    44401 tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac
    44461 tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat
    44521 gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg cgtccacggc
    44581 gttaaaacca atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat
    44641 ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg
    44701 cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc
    44761 tgccgtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc
    44821 caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcag
    44881 atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc
    44941 ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc
    45001 tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggctg tcacctgcgc
    45061 ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttgagat cgtattggct
    45121 tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc
    45181 ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat
    45241 gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga
    45301 aataaaactt taatcaccaa tattgaaagt cagatgctat ttttttaccc acaaaagaat
    45361 ataacaaact gattaataac caaaattaac gaaaaacgcc ctctgttcac tcgaaacaaa
    45421 aatgtgatac caatcacaga atacagctta ttgaataccc attatgagtt agccattaac
    45481 gcgtccacga tgttaataat aattatatta aatgttaaca aaaataaaac aaacgggaac
    45541 gcaaaataaa tattcgtttt cacagtggaa ttaactcatg aagaatgaaa agagaaaaac
    45601 gggaatagaa ccgaaggttt tctttccgcc attaataatc gtcggcatac tttgttggct
    45661 tacagtcaga gatctggatg cggcgaatgt cgttattaat gctgtattca gttacgtcac
    45721 caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg gttggttctg
    45781 gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccgccag aatttagcac
    45841 cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac tgttctgggg
    45901 atcgattgag atctactact acatctccac cccgccgttt ggcttagaac cgaactcgac
    45961 aggagcgaaa gagttggggc tggcttacag cttgttccac tggggacctc tgccgtgggc
    46021 cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca aaatggaagt
    46081 gattcgcccc agctcgacac tggtgccgct ggcaggtgaa aaacacgcca aagggttgtt
    46141 cggtaccatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg gtaccagtct
    46201 gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca ttccgcatac
    46261 cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca tttgcgtcgc
    46321 ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc tgagcttcct
    46381 gatgctgggt tgggtgttca tcgtcagcgg tgccagcttc atcatgaact acttcaccga
    46441 ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg ttctataccg atcccatcgc
    46501 taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg tgatttacgc
    46561 catccagatg agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc gtgaactgtg
    46621 cttcggcatg gtgatggggc tgacagcatc cacctggatc ctgtggaccg tgctcggtag
    46681 taacactctg ctgttgatgg ataaaaacat catcaacatt ccaaatctga tcgaacagta
    46741 cggtgtggcg cgcgccatca tcgaaacctg ggccgctctg ccactcagca ccgccaccat
    46801 gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg cctgctctta
    46861 taccctggcg atgtctactt gccgcgaagt acgcgatggt gaagaaccac cgctgctggt
    46921 gcgtatcggt tggtcaattc tggttggcat tatcggtatt gttctgctgg cgctcggcgg
    46981 cctgaaaccg attcaaaccg ccattatcgc cggaggatgc ccgctgttct tcgtcaacat
    47041 tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt aattaacccc
    47101 caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg aactgtttgt
    47161 cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg ccgagtgcga
    47221 ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg gtatcgacag
    47281 tctgctgatc cctgaagagc acggtggtct ggacgcgggg tttgttactc tcgccgccgt
    47341 gtggatggag ctgggacgtc tgggggcacc aacctatgta ctgtaccagc tgccgggcgg
    47401 gttcaatacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta tggctttccg
    47461 cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg gctccgacgt
    47521 gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta atggtagtaa
    47581 gtgttttatt accagtagcg cctacacccc gtacatcgtg gtgatggcgc gcgacggggc
    47641 ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac cgggcatcaa
    47701 agtgaccaaa cttgagaagc tcggtctgcg tatggatagc tgctgtgaaa tcacctttga
    47761 cgatgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggct ttaaccgcgt
    47821 caaagaagag ttcgaccatg aacgtttcct ggtagccctg accaactacg gtacggcgat
    47881 gtgcgccttt gaagatgctg cgcgctacgc caaccagcgc gtgcagtttg gcgaggctat
    47941 tggtcgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat taaactccat
    48001 gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca cctctggcga
    48061 tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg atagcgcaat
    48121 gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct tctggcgtga
    48181 cctgcgtgta gaccgcgttt ccggaggatc tgacgaaatg cagatcctga cgctgggtcg
    48241 tgcggtgctg aagcaatacc gctaagtccg tgcgctgcct gatgcgacgc tgacgcgtct
    48301 tatcaggcct acgggagcac gaatgtaagc cggataaggc gtttacgccg catccggcag
    48361 tcatgcgcta cataaatttt tcaggagcta ataatggatc atctacccat gccgaaattc
    48421 ggaccgctgg ccggattgcg cgttgtcttc tccggtatcg aaatcgccgg accgtttgcc
    48481 ggacaaatgt tcgcagaatg gggcgcggaa gttatttgga tcgagaacgt cgcctgggcc
    48541 gacaccattc gcgttcaacc gaactacccg cagctctccc gccgcaattt gcacgcgctg
    48601 tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc tgaaattaat ggaaaccacc
    48661 gatatcttca tcgaagccag taaaggtccg gcctttgccc gtcgtggcat taccgatgaa
    48721 gtactgtggc agcacaaccc gaaactggtt atcgctcacc tgtccggttt tggtcagtac
    48781 ggcaccgagg agtacaccaa tcttccggcc tataacacca tcgcccaggc cttcagtggt
    48841 tacctgattc agaacggtga tgttgaccag ccaatgcctg ccttcccgta taccgccgat
    48901 tacttttctg gcctgaccgc caccacggcg gcgctggcag cactgcataa agtgcgtgaa
    48961 accggtaaag gcgaaagtat cgacatcgcc atgtatgaag tgatgctgcg tatgggccag
    49021 tacttcatga tggattactt caacggcggc gaaatgtgcc cgcgcatgag caaaggtaaa
    49081 gatccctact acgccggttg cggtctgtat aaatgtgccg acggctacat cgtgatggaa
    49141 ctggtgggca ttacccaaat tgaagagtgc tttaaagata ttggcctcgc acatctactc
    49201 ggtacgccgg aaatcccgga aggcactcag cttatccacc gtatcgaatg cccttacggc
    49261 ccactggtag aagaaaaact cgatgcctgg ctggcggcac ataccatcgc agaagttaaa
    49321 gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac tgaccgtacc ggaactggaa
    49381 agcaatccac agtatgttgc ccgtgaatca atcactcagt ggcaaacgat ggatggtcgc
    49441 acctgcaaag ggccgaacat catgccgaaa ttcaaaaata accccggaca aatctggcgc
    49501 ggaatgccct cacatggcat ggacacggct gccattttga aaaatatcgg ctacagcgaa
    49561 aacgacattc aggagttggt cagcaaaggt ctggccaaag ttgaggacta aacgcgtggc
    49621 gtcagtcggc ttgcctgact agcgcccgcc caataacaaa cgaacaaaaa tggatagagg
    49681 tgcaatggat atcattggcg gacaacatct acgtcaaatg tgggacgatc tggcggacgt
    49741 ttacggtcat aaaacggcgc tgatttgtga atccagcggc ggagtcgtta accggtatag
    49801 ttatcttgag ttaaatcagg agattaaccg cacggcaaac ctgttttata cgctggggat
    49861 tcgcaaaggc aacaaggttg cactacatct cgacaactgc ccggaattta tcttttgctg
    49921 gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc tgttacgcga
    49981 ggaaagcgag tggatcttgc aaaatagcca ggcgtgcctg ctggtgacca gtgcgcaatt
    50041 ttatcctatg tatcaacaga ttcagcagga agatgccact caattgcggc acatttgcct
    50101 gacagatgtg gcacttcccg ctgatgatgg agtgagttca tttactcaac tgaaaaatca
    50161 acaacctgcc accttgtgct atgcaccgcc gctatcgact gacgatacgg cggaaattct
    50221 cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg attacccatt acaacctgcg
    50281 cttcgctgga tattactccg cctggcagtg tgcactgcgt gacgatgacg tctacatgac
    50341 ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg gcgatggcgg cgttttctgc
    50401 cggggccacc tttgtgctgg tcgagaaata cagcgcccgc gccttctggg gacaggcgca
    50461 gaagtaccgc gccaccatta ccgaatgtat tccgatgatg atccgtacgt tgatggtgca
    50521 gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa gtgatgtttt atctcaactt
    50581 gtcggagcag gaaaaagacg cattttgtga acgcttcggc gttcgcttgc tgacgtctta
    50641 tgggatgacg gaaaccattg tgggcattat cggcgatcgc cctagcgata aacgacgctg
    50701 gccgtcgatt ggtcgggcgg ggttttgcta cgaagcggag atccgcgacg atcacaatcg
    50761 cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa ggcgtaccag ggaaaaccat
    50821 cttcaaagag tattttctca acccgaaagc cactgcgaaa gtgctggaag ccgacggctg
    50881 gctacatacc ggcgataccg gataccgcga cgaagaaggc tttttttatt tcgtcgatcg
    50941 ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc tggaaaatat
    51001 catcgccacc catccgaaaa ttcaggacat cgtggttgtg ggtattaaag attcgattcg
    51061 cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt gaaacattga gcgaagagga
    51121 attttttcgc ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt atctggagat
    51181 cagaaaagat ctgccacgta attgctcggg gaaaataatt agaaagaatc tgaaataaat
    51241 gaaacgtcag ggaactaccc tgccagctaa taatcacgcc ctaaaacaat atgcattttt
    51301 tgcagggatg ttgtcgtccc taaaaaagca aaaatggaga aaaggaatga gtgaatcatt
    51361 acatctgacc cgcaatggat caattctgga aattaccctt gatcgcccaa aagcgaatgc
    51421 tattgatgca aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc gtgacgatcc
    51481 gcaattacgt gtcgccatta ttaccggtgc cggagagaaa ttcttttccg cgggctggga
    51541 tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac tttggcccgg gtggttttgc
    51601 gggattaacc gaaattttca atctcgacaa accagttatc gcagctgtga acggctacgc
    51661 ctttggcggc ggtttcgaac tggcgctggc ggcagatttt attgtttgtg ccgataacgc
    51721 cagcttcgcc ctgccggaag ccaaactggg catcgttcct gacagcggcg gtgtgctgcg
    51781 tctgccgaag atcctgccgc ctaccatcgt caatgaaatg gtgatgaccg gcagacgaat
    51841 gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc gtggttagcc aggcggaact
    51901 gatggataac gcccgcgaac tggctcagca gctggttaac agcgccccgc tggcgattgc
    51961 ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg gtagaagaag cgtatcgcta
    52021 tattcgcagc ggcgtgttga aacactatcc atcggttctg cattcggaag atgccattga
    52081 agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt aacaaccgtg
    52141 agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt tgtccatccc
    52201 agtgccgtct tgattggcga tgtgattgtg ggagccggtg tctacatcgg cccactcgcc
    52261 tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg gagccaatat tcaggatggc
    52321 tgcattatgc atggctactg cgacactgac accatcgttg gggaaaacgg ccatatcggg
    52381 catggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg gatgaacagc
    52441 gtaattatgg atggcgcggt cattggcgaa gagagcattg ttgccgcaat gagctttgtc
    52501 aaagcaggct ttagtggcga gaaacgccag ttgttgatgg gtacgccagc ccgcgctgta
    52561 cgcagtgtta gtgacgacga gttacactgg aaacggttga ataccaaaga gtatcaggat
    52621 cttgtcgggc gctgtcatgc atcgttacat gaaacgcagc cactgaggca aatggaggaa
    52681 aatcgccccc gtttgcaggg gacgacggat gtgacgccta aacggtgatc ttgccggatg
    52741 cgacctggtt gctggataag atgctacaac atcgtatctg gcaagcagga cgcccggtat
    52801 caacgagata aaattaacga cgcatactct ttgacaacat catctgccac ttctgctcgc
    52861 ggttaagttc cgtgagcgga acggcaggaa tacgcggtgt ctttcttacg ccgactaatt
    52921 tttcccgact gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg accactttaa
    52981 ccagtcgctg acactggcat ccccgccctt ccagcttatt agggatcatc ttaacttcgc
    53041 agcttatttc tgtgacttcc gacagaatat aagtcagggt attaaccgcc ttgctgtgtt
    53101 caatatcgaa atgaatagag atctctcttg ctatcaccca ccgattttca gccatcatcc
    53161 attcggcgat taacaagtag agtggttttt caacatatcc ttcacacata cgaactccag
    53221 tttcacaaat taaaagtcta gctatgactc gttttgaagc aattaaacaa ggccatatta
    53281 aaattgtgga tatatcgata gtatgcaact tcaccgtcga taagtgtgag ctaaatcctg
    53341 cttatgtcat caaaaatatc gattccccaa aagatttact taatgggcaa aaaaaaacgg
    53401 tcctcatcag agaaccgtat tagttattcg atcaaattag cggatgaaaa atatccgcca
    53461 cgacacgcta ttatttgatc tgtgcgtgca tttcctgcac cgaaattact ttttcagtcg
    53521 catcggcatt cagcgccatc gcggtagcga aaccaccgtt cagggtggtg tcataatgca
    53581 ctttatattg cagcgcactg cgacggatca cgcgggagtc ttcaatcgca cgacggcctg
    53641 aggtggtgtt gatgatgtag gtatattcgc cattcttgat acggtcctga atgtgcggac
    53701 ggccttcatg caccttgttt accagacgcg gattgatacc cgcttcgccc agcacaatcg
    53761 ccgtgccgtg ggtcgcatcc agctcgaagc cctgtttcag cagttttgcc gccaggtcca
    53821 ccacgcgttc tttatcgcct tcgcgcacgg aaagcagcgc acgaccgtgt ttcttcatgg
    53881 tggagttgct gcccagctgc gctttggcga acgcttcagc gaaggtgcgg cccacgccca
    53941 tgacttcccc ggtagagcgc atttctggcc ctaacagcgg gtcaacgcct gggaatttat
    54001 tgaacggcag caccacttct ttcaccgagt agtacggcgg aataacttct ttggttacgc
    54061 cctgctcagc cagcgatttg ccagccatca cacgcgccgc cacttttgcc agcggtacgc
    54121 cggtggcttt ggagacgaac ggaacagtac gcgccgcacg cgggtttact tcaatcaggt
    54181 aaacttcgtt gtttttcacc gcaaactgca cgttcatcag accgcgcacc tgcaattcga
    54241 aggccagttt ctgcacctgc tggcgcatca catcctgaat ttcctgactt aaggtgtacg
    54301 ctggcagcga acatgcggag tcaccggagt gcacacccgc ctgctcgatg tgctccatga
    54361 tgccgccaat cagcaccatt tcgccgtcgc agatggcatc cacgtcaact tctactgcgt
    54421 catcaaggaa atgatccagc aacactggcg catcgttaga cacgctaacc gccgtctgga
    54481 agtagcgacg caggtcagct tcgtcataga cgatttccat cgcccgaccg ccgagaacgt
    54541 aagacggacg caccaccagc gggtagccaa tctcttttgc cttctcaact gccatttcaa
    54601 tagcagtaac ggtggcgttc gccggttgtt tcagtttcag acggtcaacc gcatgctgga
    54661 agcgttcacg gtcttccgca cggtcgatag catccgggct ggtgccgata accggtacgc
    54721 cagcggcttc cagtgcacgc gccagtttca gcggggtctg accgccgtac tgaacgataa
    54781 cgcctttcgg cttctcaata cgcacgattt ccagcacatc ttccagagtt accggctcga
    54841 agtagaggcg atcggaagtg tcgtagtcgg tagagacggt ttccgggtta cagttaacca
    54901 taatggtttc gtaaccgtct tcacgcagcg ccagcgaggc gtgtacgcag cagtagtcga
    54961 attcgatacc ctgaccgata cggtttggac cgccgccaag caccatgatt ttttcacggt
    55021 cggtagacgg attcgcttcg cactcttctt cataagtgga gtacatgtaa gcggtgtcgg
    55081 tggcaaactc tgccgcacag gtatccacgc gcttgtagac cgggtgcagg tcatattggt
    55141 cacgcagctt acggatttcc gcttcgcgta cgcccgccag ttttgccaag cgcgcatcgg
    55201 caaagccttt gcgtttcagc tggcgcagga attcagcgtt caggccagtg atgcccactt
    55261 ccgccacttt ctcttccaga cgcaccagtt cttcaatctg taccaggaac cagcggtcaa
    55321 tgttagtcag gttgaagacg ccgtccacag acaggcccgc gcggaaagca tcggcgatgt
    55381 accagatacg ctctgcgcct gcgtctttca gttcgcgacg gattttggtt aacgcttccg
    55441 ggtcatccag gctcactttc gggtcgaagc cagttgcgcc cacttccagg ccgcgcagcg
    55501 ctttttgcag ggattcctgc tgcgtgcgac caatcgccat cacttcgcca accgatttca
    55561 tctgagtggt cagacggtcg ttagcaccgg cgaatttttc gaagttgaag cgaggaattt
    55621 tagtaaccac atagtcgatg gacggctcga aggaggccgg agtacgtccg ccagtgatgt
    55681 cgttcatcag ttcgtctagg gtgtaaccca ccgccagttt cgccgccact ttagcaatcg
    55741 ggaaaccggt cgctttcgac gccagcgccg aagaacggga cacgcgtggg ttcatttcga
    55801 taacaatcag gcgaccgttt ttcgggttca ccgcaaactg gacattggaa ccaccggttt
    55861 caacgccgat ttcacgcagc accgccatcg aggcgttacg catgatttga tattctttgt
    55921 cggtcagcgt ttgggctggc gcgacagtga tggagtcacc ggtgtggatg cccatcgcat
    55981 caaagttttc gatagagcag acgatgatgc agttgtcgtt tttatcacgc accacttcca
    56041 tctcgtactc tttccagccg atcagcgact catcaatcag caactctttg gtcggagaga
    56101 gatccagacc gcgggcgcaa atttcttcaa actcttcacg gttataagcg ataccgccgc
    56161 cgctaccgcc catggtaaag gatgggcgaa taatgcacgg gaagcccacg tcagcggcaa
    56221 cagccagcgc ttcttccatc gtgtgtgcga taccggaacg cgcggtttcc agaccaattt
    56281 tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt atcaatcgca tcggcagtgg
    56341 caccaatcat ggtgacaccg aactcttcca acacgccctg acgttccagc tccagcgcgc
    56401 agttcagcgc cgtctgaccg cccatggttg gcagcaccgc gtccgggcgc tctttttcaa
    56461 taatcttgcg taccacttcc cagtgaatcg gctcgatgta ggtcgcatcg gccatttccg
    56521 ggtcggtcat gatggtcgcc gggttggagt tcaccagaat gacgcggtaa ccctcttcgc
    56581 gcagggcttt acatgcttgc gcgccagagt agtcaaactc acacgcctga ccgataacaa
    56641 tcgggcccgc acccagaatc aggatacttt ttatatctgt acgttttggc atggctcttt
    56701 tactcctgat tacttagcgg ttttacggta ctgctcaatt aacttgataa agtggtcgaa
    56761 caacggcgcg gcgtcgtgtg gaccagggct ggcttcaggg tgcccctgga agctgaacgc
    56821 cggcttatcg atgcgatgaa tgccctgtaa cgtaccgtcg aacagggatt tatgcgtgac
    56881 acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa ccgtggttct gggcggtgat
    56941 catcaccacg tttttctcca catctttaac cggatggttg ccgccgtggt gaccaaattt
    57001 cattttgaca gtcttcgcac cgctcgccag cgccagcagc tgatgaccga gacagatgcc
    57061 gaataccgga atatcggttt cgaggaactt ctggatggcg gtaatggcgt aatcgcacgg
    57121 ggccgggtcg ccaggaccgt tggagaggaa gatgccgtct ggattcattt tcagcacatc
    57181 ttccgcagaa gtttgcgccg gaacgatggt caggcgacag cctctatcca ccagcatccg
    57241 caggatgttg cgcttggcac caaaatcata agccacgacg tggaacggca gctcgtcttc
    57301 ttttttcgct tctggcaggc cgccggtcaa cgtccagctc ccttgtgtcc agctataggc
    57361 ttctgcggtg gtcacttctt ttgccagatc catgccgttc agacctggga acgcgcgggc
    57421 tttttctaac gccagcgccg catccgggtt atcgcctgcg ataatgcagc cattctgtgc
    57481 gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg atatcggcaa tcgccacgat
    57541 gttatggcgc ttcaggtaag aagagaggtc ttcggtatta cggaagttgc tggcaatcag
    57601 cggcaggtcg cgaatcacca gaccttgtgc atgtacctga gaagattctt catcggcgtc
    57661 attagtgccg acattgccaa tatggggata agtaagagta acgatttgac gagaatagga
    57721 aggatcagtg aggatttctt gataaccggt cattgaagta ttgaaaacga cttccccaac
    57781 cgccgaacct gttgccccta tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa
    57841 tagcgctgac ttaatcaaaa caccctccag agaatactca ctcactttat ttgcatatta
    57901 attcacagtg atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg
    57961 cggcattctg gagatatggg cgctaaaagt caacttaatg gcaggatttt ttatattatt
    58021 ttgtggctca tgcatgtttt acaggcatta agcgacaaaa acatcatcta acctgttctg
    58081 gaaaacgctt gcgcagcaag aagattgcat gaaataaaaa cgagcaggaa aaagtggacc
    58141 aaatggtcaa aatttacatt aaaacaacaa aaatagaaga cgaaacatgt aatttttggc
    58201 agttatagaa aatttaaaag cataacacca aaaataaaag ggctaaaaat taaagccctt
    58261 tataattaat aaattaaatg tgttactttt gcatcataat aaacattttg tggttacaaa
    58321 ttattgagat caagcacatc tcgcatatca aaaagaccac tttccttacc actcaaccac
    58381 aaagccgttc ttaccgcacc gttagcaaat gtcatgcggc tggacgcctt atgggtgatc
    58441 tccagacgct cgccaatatc ggcaaacatc gcggtatgtt cgccaacgat gtcacctgca
    58501 cgcacggtag caaaaccaat ggtacctggc acacgttcac cggtatggcc ttcacgactg
    58561 tagaccgcgc aatctttcag atctttatca agggcgtggg cgatcgcctc tcccattgcc
    58621 agtgcggtgc ctgacggcgc atcaacttta tgtctatgat gtgcttcaat aatttcgata
    58681 tcggtgtagt cacccatcac tttggctgct ttctccagca gcttaagcat gacgttaacg
    58741 ccaacgctaa aattggcggc aaagacaatc gcaatatcgg cggcggcgtc acgaattgcc
    58801 tgtttaccgg cttcgtcaaa ccccgtagtg ccgatcacca tccctttgcc atgctgccga
    58861 caaaaagcga gatggttcag cgtaccttcc ggacgggtaa aatcgataaa cacatcaaaa
    58921 tcatctttta tcgcatcaag actgctttgc acggtaacgc ctgttttccc tgcaccagcc
    58981 agctcaccgg cgtcgctgcc cagtaaagaa gatccttcgc gctccagcgc cgcgcccagc
    59041 tgcacgcctt ctaatgccag tgccgcctga atcaactggc ggcccatacg ccccccggct
    59101 cccgcgatgg caacgcggat gtttgcatca tgcatagcta ttctcttttg ttaatttgca
    59161 tagaccgttt tcagagtaac cagcgcatcg ctacgccgcc agccaaaaac gctgataatt
    59221 agaaattaat gataaacagg gagggatatc agtgaaaggc atgagtcgat gaatgactgc
    59281 atgccgataa catgtgacag gttacggcac cagagccagc acctcagcca cccactgttg
    59341 gaagcctttt acattcagat ccaatgccac ctgtacattg gctggtttgc ccaggcaacc
    59401 gtcgatatca accaccgtcg tgcctgaggt aaattcgcct tgagtttcta ccgcgacaaa
    59461 acacggcttg agggtgaata agtccgggcg caccagccag gcgatggcgc agagatcgtg
    59521 cattcgcaga ccgctttgca tactgccgct acggtagtgg ctaaacaggg cgtggagcat
    59581 ttttccggtg cgattcaact ccggtaaagt agcgagatag tcaggggtta atattgcctg
    59641 attggtgaca tccagaccgc acatgacaat ttcgatgccg ctacggaaga cacaggcagc
    59701 cgcttctgga tcggcagcga tattaaactc ggcatttggt gtacagttgc cgcgaccagc
    59761 agaaccgccc atgatcacca gacgacgaat atacggttta cattccgggc attgtaaaag
    59821 taacagcgca atattggtta acgggccgat ggccaccagg gtaacgggct ctggtgcacg
    59881 catcagggca tcccgaatcg ccagaaacgc cggtatcccg agtggctttc ggttgtgctc
    59941 aacaaagtcg tagccagcca ttcccgattc gccgtgcaca gatgctgcat cacgcggtgc
    60001 gcgtaccagc ggcacagcgg ctccctgggc gagcggaatc tccgcattcc agaaatgcag
    60061 cagttgcagg gcattgcggg tagttttctc aaccgagaca ttacccgcga cggtggtcat
    60121 cagttgcagg tcgagttcgg gggcaaaaat cgcggcggca atggcgacgg catcgtcaat
    60181 gccggggtca gtatcgagga agataggtaa acgcatgttt tcttcataaa aaatgccggt
    60241 aacaagaccg gcattttcgc ataacttagg ctgctaatga cttaatcgac ttcacgaata
    60301 tcgacacgca gctctttcgg cacttcgaaa acaatatttt cttcacggcc ttccagcgga
    60361 atggcttcac caccacccaa ctgctgcaaa cgtgccacca cattctgcac cagaatatcc
    60421 ggagccgatg cgcccgcagt cacgccgacg catttaacct ctttcaccca ctcttcctgg
    60481 atatctttcg catcgtcaat caaaaacgcg tgtttgccca tacgctgggc cagctccgcc
    60541 agacggttgg agttggagga gtttttcgaa ccgaccacca acacaacttc cgcctgttct
    60601 gccagggcgc gtaccgcttc ctgacggtta gtcgtggcgt agcagatgtc atctttgcgc
    60661 ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga tcacatcaga cgtgtcatcc
    60721 accgacagcg tggtttgggt cataaaggag agcttctctt cgtttttgac cgtcagtttc
    60781 cacacatcgt ctggcgattc gactagatac attccccctt cggggttgct gtactgcccc
    60841 atcgtccctt ccacttccgg gtgcccggcg tggccgatga gaatagattc ttcgccacgg
    60901 cgactggcgc gggcaacttc catatgcact ttggtcacca gcggacaggt ggcatcaaac
    60961 accgtcaaat cgcggctttt cgcttcgtta cgaaccgcct gagaaacacc gtgtgcggag
    61021 aaaatcagga tcgcgccgtc cggcacttcg ctaatctgct caataaagat agccccacgc
    61081 tcgcgcaagc tatcgaccac gtagcggtta tgcaccactt cgtgacggac atatatcggt
    61141 gcgccgtaaa tggccagcgc gttttcaaca atgctgatag cgcggtctac cccggcacaa
    61201 aaaccacgcg ggttggccaa caggatctgc atgttacgcc tccagtgccg gatcgatttc
    61261 cagcacttca atatcaaaat gaacggtctg cccggccagc ggatggttga aatcaacggt
    61321 aatggagtcg ccgttaattt cgcggatcac accaggcatc tcactgccat ccattgcggt
    61381 aaaaagcatg attgcgccaa tttctggctc gcctgcatcc ataaattcac ggcgggagaa
    61441 gtactgaatc aggtccggtg acggcacgcc aaacgccgca tctggctcca gcgagaaggt
    61501 ggttttgtcg cccactttca gccccagcag gtgttgctcc agcccttcag aaagagaagc
    61561 atcgcccagg cggaacagcg ccggtttacc gttgttgcga gtagactcgg cggtggtgcc
    61621 atcgtcgagt tttagcgtga agtgcaccag gacggcgcta ttgctctgta cagattcaga
    61681 catgcaggtt tgctcttatt tttgaggatt tttgccggat gcggcctacg atttcgtagc
    61741 aatctgtagg ccgcatccgg caggatttat tattgttttt tcgctttgga aggtaaaaac
    61801 ccttccagca cgatcagtgc cgcaccgaca caaatggcag tatcggcaag gttgaaggtg
    61861 gcgaagtgcc agtcgccgac gtagaagtcg atcatatcga caacgaagcc gtgccacagg
    61921 cggtcgaaca ggttgcccag cgcgccgcca ataatcagcg cgtaagcgat attgtttagc
    61981 ttctgcgtgg ccttcgagcg atacatcatc actgccagga gcacgctaat accaatcgca
    62041 ataccggcaa agaaccaacg ctgccagccg ccgctatcgg caaggaaact aaacgccgcg
    62101 ccatagttac gcgcataatg cagattaagc gacgggaaca gcgggaccgt atcccccaga
    62161 gcaaagttct ggaggatcag gtatttgctg cccagatcga taatcagcac gactaccacc
    62221 agccacagcc agcgtagccc tgttgaacag atcgattgac tcatcaggca aacttacgtt
    62281 tttcaccgtc accggcgacg ttgctgacac agcggccgca gatttctgcg tgttccgcca
    62341 ccttgccgac atcctgggtg tagtgccagc agcgtgggca cttctcacct tcggctttac
    62401 tcaacgcgac tttcagccct ttgagtactt cgctctgctg agcatcagca ggtgcgtcgt
    62461 tatagtctgc aacggtagcg ccggaggtca acaggacaaa tcgtaattca tcgcccagcg
    62521 cggtcagttt cgccgccagt tccggttctg catacaaggt tactgccgct tccagcgagc
    62581 cacccacttt cttgtcggca cgcgcttgct caatgacttt gttcacttcg ccacgcactt
    62641 tcaacagctc gtcccagaac gcatcgttca ttgcttcact gtctgccaga ccaaacaggc
    62701 cttcgtacca ctcaccggtg aagacgtatt tttcacgttc gcccggcaga tagccccaca
    62761 cttcatcagc ggtgaaggag aggattggtg ccatccagcg caccagcgct tcagcgatgt
    62821 gatacagcgc agtctggcag ctacgacgcg ccacgctatc cgctttggcg gtgtactgac
    62881 ggtctttgat gatgtcgagg tagaaggaac ccatctcaac ggagcagaag cgcatcagac
    62941 gctgtaccac ttcgtggaaa tcgtatgctt cgtacgcctt gaggatgtct tcctgtgccg
    63001 ctttcgcaca acctacggcc cagcgatcca gtaccaccat ctcttccggt ttcaccatat
    63061 cttttgctgg atcaaaacca ttcaggtttg ccagcaggaa gcgcgcggtg ttacggatac
    63121 gacgatagct atcggcagca cgtttcagga tctcgtcaga aacggccatt tcaccggtgt
    63181 agtcggttga tgccacccac agacgcagaa tatccgcgcc cagtttgttc atcacatcct
    63241 gcggcgaaac ggtgttgccg atggatttag acatcttgcg accctgacca tccacggtaa
    63301 agccgtgggt cagtacctga cgatacggcg ctttgccttt catcgcggtg gagatcatta
    63361 gggaagacat gaaccaacca cggtgctggt cagaaccttc cagatacatg tccgctgcgt
    63421 gaccggcaaa ttccggacgc acgtcaacaa cagaagagtg ggtagatccg gagtcaaacc
    63481 atacgtcgag agtatccggc actttcacat actggtcagc ttcgtcgccg aggatctctt
    63541 tcgcatcgag atcccaccac gcctggatgc cgtcaacttc aacgcgttta gccacttctt
    63601 ccatcagttc gagggtacgc ggatgcagct cttccgtgtc tttgtgcacg aacagtgaca
    63661 tcggtacgcc ccaggtgcgc tgacgggaga tacaccagtc aggacggtta gcaaccatcg
    63721 actcgatacg cgcctggccc cagtccggga gccactgcac gcctttgatc tctttcagtg
    63781 actgcgcacg cagacctttc tgatccatgc tgacgaacca ctgcggtgtc gcgcggaaga
    63841 tgatcggcgt tttgtgacgc cagcagcacg gatagctgtg ctgcattttc tcaacgtgca
    63901 gcagcgcgcc tttttcctgc agcagcgcaa cgacgatgtc gttcgctttg aagacgttca
    63961 cgccatccag cgtcggataa gtgcccggca gataagtgcc gtccgggcca accgggttag
    64021 cggtttccag gccgtatttc tgaccgatca catagtcgtc cgggccgtgg ccaggcgcgg
    64081 tgtgaacggc accggtaccg gcatccaggg taacgtgatc gccgaggatt gccggaacgt
    64141 cgaagcccat aaacggatgg gtaaagcgca gcagctcaag ctctgcacct tttaccgtgc
    64201 cgagaatggt gtaatcggtc acgccgatac gctgcattac gctttcaacc agatctttcg
    64261 ccagaatcac ggcctgaccg tcgatctgca ccagcgcata gtcgaaatca ggtgcaatag
    64321 agattgcgcg gttcgcaggc agagtccacg gcgtagtggt ccagatcacc agcgagattg
    64381 ggccgttaac gttgcttacg ccaaatttcg ctttcagcgc atcctgatcg accgcctgga
    64441 aagcgacgtc aatggacgga gaagttttgt cgtaatactc aacttccgct tctgccagtg
    64501 cagaacggca gtctacgcac cagtgcaccg gcttcgcgcc tttgtgcagg tgaccattgc
    64561 cgatgatttt gcccagcgcg cggatgatgt tggcttcagt tttgaagtcc atagtcagat
    64621 acgggtgcga ccagtcgccc agcacgccca gacggataaa gtctttgcgt tgaccgtcaa
    64681 cctgggtcgc cgcgtattcg cggcacttgg cgcggaactc ggcggcggtg aatttctcac
    64741 ccggcttacc gtattcttgc tctactttca gctcgatcgg cagaccgtgg cagtcccagc
    64801 caggcacata cggcgagtca taaccggaaa gccctttgga cttcacgata atgtctttca
    64861 gaatcttgtt aaccgagtga ccaatatgaa tgctgccatt cgcataagga gggccatcat
    64921 gcagaatgaa ggtttttttg ccttttttag ccgcacggat gatgccgtac agatcatcat
    64981 cagtccaacg cgccagcatt cccggttcgc gcttggcgag atcgccacgc atcgggaacc
    65041 ctgtttccgg caaattcagg gttgatttat agtcactcat cagattctcg gttccgtgtt
    65101 tcggtttgat tacataacag gcttaagccg gttttgttag cccaaaaaat tcgcgggcgg
    65161 ttaattcatc acgcgcaatc tgcgctttca gttcgtccag cgacgcaaat cgctgctcat
    65221 tgcgtatttt tttacgcagc actacttgta tatggcgacc gtaaaggtcc attgcaacat
    65281 ctaacaaatg cacttccagc tgctggcgaa taccggcaac cgttgggcgt gttccgatgt
    65341 ttgccacgcc gggtaacggc ttttcaccga ggcccagcac ttctaccgca taaacccctt
    65401 tcaccgggga aacctgacgg cgtagcggta cattcgccgt cgggaaacct atagtgcgcc
    65461 ctaattcatc accgtggact acacgcccgg agatggcaaa cgggtgcccc agtaaacttt
    65521 ctgccagagc cagattgtca tcagccagcg cctgacgc
//
LOCUS       DAJTRH010000011        63465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTRH010000011 DAJTRH010000000
VERSION     DAJTRH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            82..900
                     /gene="ybhA"
                     /locus_tag="OO339_000772"
     CDS             82..900
                     /gene="ybhA"
                     /locus_tag="OO339_000772"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5925510.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(901..1959)
                     /gene="modC"
                     /locus_tag="OO339_000773"
     CDS             complement(901..1959)
                     /gene="modC"
                     /locus_tag="OO339_000773"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5925511.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1962..2651)
                     /gene="modB"
                     /locus_tag="OO339_000774"
     CDS             complement(1962..2651)
                     /gene="modB"
                     /locus_tag="OO339_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5925512.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2651..3424)
                     /gene="modA"
                     /locus_tag="OO339_000775"
     CDS             complement(2651..3424)
                     /gene="modA"
                     /locus_tag="OO339_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5925513.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3591..3740)
                     /gene="acrZ"
                     /locus_tag="OO339_000776"
     CDS             complement(3591..3740)
                     /gene="acrZ"
                     /locus_tag="OO339_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5925514.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3869..4657
                     /gene="modE"
                     /locus_tag="OO339_000777"
     CDS             3869..4657
                     /gene="modE"
                     /locus_tag="OO339_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5925515.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4725..6197
                     /gene="modF"
                     /locus_tag="OO339_000778"
     CDS             4725..6197
                     /gene="modF"
                     /locus_tag="OO339_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5925516.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6458..7474
                     /gene="galE"
                     /locus_tag="OO339_000779"
     CDS             6458..7474
                     /gene="galE"
                     /locus_tag="OO339_000779"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5925517.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7484..8530
                     /gene="galT"
                     /locus_tag="OO339_000780"
     CDS             7484..8530
                     /gene="galT"
                     /locus_tag="OO339_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5925518.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8534..9682
                     /gene="galK"
                     /locus_tag="OO339_000781"
     CDS             8534..9682
                     /gene="galK"
                     /locus_tag="OO339_000781"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5925519.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9676..10716
                     /gene="galM"
                     /locus_tag="OO339_000782"
     CDS             9676..10716
                     /gene="galM"
                     /locus_tag="OO339_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5925520.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10919..11671
                     /gene="gpmA"
                     /locus_tag="OO339_000783"
     CDS             10919..11671
                     /gene="gpmA"
                     /locus_tag="OO339_000783"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5925521.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11838..12890)
                     /gene="aroG"
                     /locus_tag="OO339_000784"
     CDS             complement(11838..12890)
                     /gene="aroG"
                     /locus_tag="OO339_000784"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5925522.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13206..13586
                     /locus_tag="OO339_000785"
     CDS             13206..13586
                     /locus_tag="OO339_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5925523.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13700..14641
                     /gene="zitB"
                     /locus_tag="OO339_000786"
     CDS             13700..14641
                     /gene="zitB"
                     /locus_tag="OO339_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5925524.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(14638..15357)
                     /gene="pnuC"
                     /locus_tag="OO339_000787"
     CDS             complement(14638..15357)
                     /gene="pnuC"
                     /locus_tag="OO339_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5925525.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15395..16438)
                     /gene="nadA"
                     /locus_tag="OO339_000788"
     CDS             complement(15395..16438)
                     /gene="nadA"
                     /locus_tag="OO339_000788"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5925526.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(16687..16762)
                     /locus_tag="OO339_000789"
     tRNA            complement(16687..16762)
                     /locus_tag="OO339_000789"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16727..16729),aa:Lys,seq:ttt)
     gene            complement(16766..16841)
                     /locus_tag="OO339_000790"
     tRNA            complement(16766..16841)
                     /locus_tag="OO339_000790"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16806..16808),aa:Val,seq:tac)
     gene            complement(16893..16968)
                     /locus_tag="OO339_000791"
     tRNA            complement(16893..16968)
                     /locus_tag="OO339_000791"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16933..16935),aa:Lys,seq:ttt)
     gene            complement(16971..17046)
                     /locus_tag="OO339_000792"
     tRNA            complement(16971..17046)
                     /locus_tag="OO339_000792"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17011..17013),aa:Val,seq:tac)
     gene            complement(17182..17257)
                     /locus_tag="OO339_000793"
     tRNA            complement(17182..17257)
                     /locus_tag="OO339_000793"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17222..17224),aa:Lys,seq:ttt)
     gene            complement(17422..18213)
                     /gene="cpoB"
                     /locus_tag="OO339_000794"
     CDS             complement(17422..18213)
                     /gene="cpoB"
                     /locus_tag="OO339_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5925527.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18223..18744)
                     /gene="pal"
                     /locus_tag="OO339_000795"
     CDS             complement(18223..18744)
                     /gene="pal"
                     /locus_tag="OO339_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5925528.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(18779..20071)
                     /gene="tolB"
                     /locus_tag="OO339_000796"
     CDS             complement(18779..20071)
                     /gene="tolB"
                     /locus_tag="OO339_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5925529.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20204..21445)
                     /gene="tolA"
                     /locus_tag="OO339_000797"
     CDS             complement(20204..21445)
                     /gene="tolA"
                     /locus_tag="OO339_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5925530.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(21510..21938)
                     /gene="tolR"
                     /locus_tag="OO339_000798"
     CDS             complement(21510..21938)
                     /gene="tolR"
                     /locus_tag="OO339_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5925531.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(21942..22634)
                     /gene="tolQ"
                     /locus_tag="OO339_000799"
     CDS             complement(21942..22634)
                     /gene="tolQ"
                     /locus_tag="OO339_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5925532.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(22631..23035)
                     /gene="ybgC"
                     /locus_tag="OO339_000800"
     CDS             complement(22631..23035)
                     /gene="ybgC"
                     /locus_tag="OO339_000800"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5925533.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23185..23478)
                     /gene="ybgE"
                     /locus_tag="OO339_000801"
     CDS             complement(23185..23478)
                     /gene="ybgE"
                     /locus_tag="OO339_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5925534.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(23478..23591)
                     /gene="cydX"
                     /locus_tag="OO339_000802"
     CDS             complement(23478..23591)
                     /gene="cydX"
                     /locus_tag="OO339_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5925535.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(23606..24745)
                     /gene="cydB"
                     /locus_tag="OO339_000803"
     CDS             complement(23606..24745)
                     /gene="cydB"
                     /locus_tag="OO339_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5925536.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(24761..26329)
                     /gene="cydA"
                     /locus_tag="OO339_000804"
     CDS             complement(24761..26329)
                     /gene="cydA"
                     /locus_tag="OO339_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5925537.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27178..29811)
                     /gene="mngB"
                     /locus_tag="OO339_000805"
     CDS             complement(27178..29811)
                     /gene="mngB"
                     /locus_tag="OO339_000805"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5925538.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(29829..31745)
                     /gene="mngA"
                     /locus_tag="OO339_000806"
     CDS             complement(29829..31745)
                     /gene="mngA"
                     /locus_tag="OO339_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5925539.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            31915..32637
                     /gene="mngR"
                     /locus_tag="OO339_000807"
     CDS             31915..32637
                     /gene="mngR"
                     /locus_tag="OO339_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5925540.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(32740..33609)
                     /gene="sucD"
                     /locus_tag="OO339_000808"
     CDS             complement(32740..33609)
                     /gene="sucD"
                     /locus_tag="OO339_000808"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5925541.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(33609..34775)
                     /gene="sucC"
                     /locus_tag="OO339_000809"
     CDS             complement(33609..34775)
                     /gene="sucC"
                     /locus_tag="OO339_000809"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5925542.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(34869..36086)
                     /gene="odhB"
                     /locus_tag="OO339_000810"
     CDS             complement(34869..36086)
                     /gene="odhB"
                     /locus_tag="OO339_000810"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5925543.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36101..38902)
                     /gene="sucA"
                     /locus_tag="OO339_000811"
     CDS             complement(36101..38902)
                     /gene="sucA"
                     /locus_tag="OO339_000811"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5925544.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39203..39919)
                     /gene="sdhB"
                     /locus_tag="OO339_000812"
     CDS             complement(39203..39919)
                     /gene="sdhB"
                     /locus_tag="OO339_000812"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5925545.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(39935..41701)
                     /gene="sdhA"
                     /locus_tag="OO339_000813"
     CDS             complement(39935..41701)
                     /gene="sdhA"
                     /locus_tag="OO339_000813"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5925546.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(41701..42048)
                     /gene="sdhD"
                     /locus_tag="OO339_000814"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(41701..42048)
                     /gene="sdhD"
                     /locus_tag="OO339_000814"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5925547.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42042..42446)
                     /gene="sdhC"
                     /locus_tag="OO339_000815"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42042..42446)
                     /gene="sdhC"
                     /locus_tag="OO339_000815"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5925548.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            42827..42934
                     /locus_tag="OO339_000816"
     CDS             42827..42934
                     /locus_tag="OO339_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925549.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43140..44423
                     /gene="gltA"
                     /locus_tag="OO339_000817"
     CDS             43140..44423
                     /gene="gltA"
                     /locus_tag="OO339_000817"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5925550.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            44814..45380
                     /locus_tag="OO339_000818"
     CDS             44814..45380
                     /locus_tag="OO339_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5925551.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            45431..47890
                     /locus_tag="OO339_000819"
     CDS             45431..47890
                     /locus_tag="OO339_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5925552.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            47905..48636
                     /locus_tag="OO339_000820"
     CDS             47905..48636
                     /locus_tag="OO339_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5925553.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            48633..49709
                     /locus_tag="OO339_000821"
                     /pseudo
     CDS             48633..49709
                     /locus_tag="OO339_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            49861..50907
                     /locus_tag="OO339_000822"
     CDS             49861..50907
                     /locus_tag="OO339_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5925554.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(50904..51695)
                     /gene="nei"
                     /locus_tag="OO339_000823"
     CDS             complement(50904..51695)
                     /gene="nei"
                     /locus_tag="OO339_000823"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5925555.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(51731..52465)
                     /gene="pxpA"
                     /locus_tag="OO339_000824"
     CDS             complement(51731..52465)
                     /gene="pxpA"
                     /locus_tag="OO339_000824"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5925556.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(52455..53387)
                     /gene="pxpC"
                     /locus_tag="OO339_000825"
                     /pseudo
     CDS             complement(52455..53387)
                     /gene="pxpC"
                     /locus_tag="OO339_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(53381..54037)
                     /gene="pxpB"
                     /locus_tag="OO339_000826"
     CDS             complement(53381..54037)
                     /gene="pxpB"
                     /locus_tag="OO339_000826"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5925557.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54060..54803)
                     /gene="ybgI"
                     /locus_tag="OO339_000827"
     CDS             complement(54060..54803)
                     /gene="ybgI"
                     /locus_tag="OO339_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5925558.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55074..56555
                     /gene="dtpD"
                     /locus_tag="OO339_000828"
     CDS             55074..56555
                     /gene="dtpD"
                     /locus_tag="OO339_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5925559.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(56705..58123)
                     /gene="phrB"
                     /locus_tag="OO339_000829"
     CDS             complement(56705..58123)
                     /gene="phrB"
                     /locus_tag="OO339_000829"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5925560.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58120..58629)
                     /locus_tag="OO339_000830"
     CDS             complement(58120..58629)
                     /locus_tag="OO339_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5925561.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            58758..59045
                     /locus_tag="OO339_000831"
     CDS             58758..59045
                     /locus_tag="OO339_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5925562.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59100..59480)
                     /locus_tag="OO339_000832"
     CDS             complement(59100..59480)
                     /locus_tag="OO339_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925563.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(59483..62869)
                     /locus_tag="OO339_000833"
     CDS             complement(59483..62869)
                     /locus_tag="OO339_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5925564.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(62888..63091)
                     /locus_tag="OO339_000834"
                     /pseudo
     CDS             complement(62888..63091)
                     /locus_tag="OO339_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15455 a        17132 c        15447 g        15431 t
ORIGIN      
        1 aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac gaaaattctt
       61 aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt agacggcacc
      121 ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc ccgcgctcgc
      181 gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat tcatcctttt
      241 tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta tttgtatgat
      301 tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc cctgcaactc
      361 attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga tgcaatggtc
      421 tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac cctgccgccg
      481 gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca acaagttaac
      541 gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca ttttggtaag
      601 catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca ggttgatatt
      661 gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc gcaaggttgg
      721 tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat gctggaagcc
      781 gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg cgccaacatt
      841 gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca cctgatttaa
      901 tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag gtttgatcgc
      961 cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc cgacttccag
     1021 ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc gcaatacgtt
     1081 acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag cctgaatgcg
     1141 gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat gctgatcgcc
     1201 cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca ctttcagaat
     1261 gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc acacttcctc
     1321 cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt ctgccagatg
     1381 gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt cccgtgtcag
     1441 ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg ccagcggttc
     1501 atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca cgcgctgttt
     1561 ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc ctaaaagcgc
     1621 caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca gattgccacg
     1681 cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac gacgcttttc
     1741 cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga ggacaatccg
     1801 ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt ttccggcacc
     1861 ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa tggtcaggca
     1921 atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc cgcccgttca
     1981 cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag cgcaatagaa
     2041 ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag ggtatacatg
     2101 gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat ggttgcacca
     2161 aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc cggtaaggtc
     2221 agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt tcttgcggcc
     2281 tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac catcagcgga
     2341 aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa ggtaatacca
     2401 aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac taataataag
     2461 taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc gagcagagct
     2521 ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa cggcaggcta
     2581 aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca ttctggatcg
     2641 gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct gcctgcggtc
     2701 ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc acaacagcaa
     2761 ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact tttacccctt
     2821 tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta cgttcgacca
     2881 gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc gtatcccatg
     2941 cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt tccggatcgc
     3001 caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg tcgatggtga
     3061 aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg agcagtgtct
     3121 gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat ttctgatcgg
     3181 cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga gtagatgacg
     3241 aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta gcaatgtcct
     3301 gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca tctgccagtg
     3361 cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac catttacgag
     3421 ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact aactcggaat
     3481 tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc ggcaggaaaa
     3541 gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa tcaatgattt
     3601 tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc accaagaccg
     3661 taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc gaataccaga
     3721 ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat tctaaccgct
     3781 aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc gttttccgtc
     3841 acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca cccttaagct
     3901 ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca ttgcgctttc
     3961 cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg cctgggatgc
     4021 cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa caggcggtaa
     4081 aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc tctatgactt
     4141 actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg ccctgccgct
     4201 gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc gtaaccagtg
     4261 gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg atgtcttact
     4321 ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg cacgtctggg
     4381 gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag gcattactca
     4441 ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta gtcatattga
     4501 gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa cactgtgcgc
     4561 cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca cggcctactt
     4621 taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat ttgttatgaa
     4681 acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca tcgttgcaaa
     4741 ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct cagctaacgt
     4801 taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag tcggcgctgg
     4861 cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc cagttttccc
     4921 acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa tggcaacgga
     4981 ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg gctgagatta
     5041 ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag ttcggtatta
     5101 ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga aaaaccctgc
     5161 tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg ttcgatggcc
     5221 tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat cagtccggta
     5281 ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc cagtttgctg
     5341 gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg ctccagcaag
     5401 cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg ccggagccgg
     5461 atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg ctgaacaatg
     5521 gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag gtgaatccag
     5581 gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta ttaagcctga
     5641 ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc cgacgtcgcg
     5701 gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc agtagtttgc
     5761 atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc tattttgatt
     5821 cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag cagtggctgg
     5881 atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt tcctggggac
     5941 agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg cttattctcg
     6001 atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt tttgttgatg
     6061 tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct gaagatgcgc
     6121 ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat cgctatgtgc
     6181 tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta agcgcactct
     6241 ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga taaaaagcgc
     6301 aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa cggctaaatt
     6361 cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct ttgttatgct
     6421 atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg ttaccggtgg
     6481 tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc atgatgtcat
     6541 cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg agcgtttagg
     6601 cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga tgaccgagat
     6661 cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag ccgtcgccga
     6721 atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc tgcgcctgat
     6781 tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg ccaccgttta
     6841 tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac cgcaaagccc
     6901 ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa aagcccagcc
     6961 ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc cgtcgggcga
     7021 tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg cccaagttgc
     7081 tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg aagatggtac
     7141 tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg tggcgatgga
     7201 aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg taggcagcag
     7261 cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta actatcattt
     7321 tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca aagccgaccg
     7381 tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca cctggcactg
     7441 gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc aatttaatcc
     7501 cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc tggtttcacc
     7561 gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac aggtgttacc
     7621 tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa
     7681 ccccgattac accgggactt acgttttcac taatgacttt gcggccttga tgtctgacac
     7741 gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc gcggtaccag
     7801 ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga gcgttgcggc
     7861 attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa aaacgtaccc
     7921 gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc cgcatccgca
     7981 cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag accgcctgca
     8041 aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc agcgcgagct
     8101 ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg tgccttactg
     8161 ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc ggatcaccga
     8221 tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga ccagtcgtta
     8281 tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg caccatttaa
     8341 tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc tgctgcgctc
     8401 cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc agcgagacct
     8461 gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc
     8521 cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg
     8581 ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac
     8641 cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg tgatcagctg
     8701 tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa atcagctcga
     8761 cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg cgaactacgt
     8821 tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg tggacatggt
     8881 gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac tggaagtcgc
     8941 ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac aaatcgcgct
     9001 taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca tggatcagct
     9061 aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct cactggggac
     9121 taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta acttcaaacg
     9181 taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg gtgcgcgttt
     9241 cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg ttgcacatga
     9301 gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg cccgcaccgt
     9361 tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt tgatggccga
     9421 gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg acactctggt
     9481 agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt
     9541 tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac agcaagctgt
     9601 cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt gtaaaccatc
     9661 acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg tcagccgtac
     9721 cgactgttaa ctttgcgtaa caacgcaggg atggtagtca ctctgatgga ctggggtgcg
     9781 actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc gctgctcggc
     9841 tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc tattggtcgt
     9901 tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt gacgctttcg
     9961 ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa acgtcgctgg
    10021 cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat
    10081 cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga cgataaccgt
    10141 atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat gactaatcac
    10201 gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt gcagattctg
    10261 gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt gaaatctgtc
    10321 gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt tcttgccgac
    10381 gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc caaaggcgat
    10441 ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct gaaggtctac
    10501 accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac accgtcgcgg
    10561 ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt tctgccggac
    10621 agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg cgaagagtat
    10681 tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt catgagggct
    10741 ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact caatattcac
    10801 aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt gctgttgctt
    10861 cgtcacggca atataatgag aattattatc attaaaagat gatttgagga gtaagcatat
    10921 ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca aagaaaaccg
    10981 tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag caaaagcagc
    11041 aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt ctgtgctgaa
    11101 acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat ggctgcccgt
    11161 tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc tgaacaaagc
    11221 ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg gttttgcagt
    11281 gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc cgcgttacgc
    11341 gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca ttgaccgcgt
    11401 gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc gcgtaatcat
    11461 cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca tgagcgaaga
    11521 agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt tcgacgagaa
    11581 tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg cgaaagcagc
    11641 agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa tgagaaagcc
    11701 gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac attgcctgat
    11761 gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac tctgacggcg
    11821 catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag ttgacgtaac
    11881 agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc gtaggccagc
    11941 ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac catcacgcca
    12001 ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac atccatctgc
    12061 tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg agctggcaga
    12121 cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta gttaggctct
    12181 ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac aatcgccgaa
    12241 tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt aatggcatcg
    12301 atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca agaaagacca
    12361 gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc gccccagctc
    12421 atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc cgctggcaga
    12481 ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc gttgatctgg
    12541 aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt ggtacgcggc
    12601 ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc acgcagcgcc
    12661 agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca cgggccaatc
    12721 accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt tcgggcatgg
    12781 gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc gacaggagga
    12841 agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat gtctgttcca
    12901 gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc cgtcagaatg
    12961 tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga aaaacgccag
    13021 attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt tgtttgttaa
    13081 ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac caatgcctta
    13141 tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca taacaggagc
    13201 atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta agccttgcca
    13261 gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag gcaaacgccg
    13321 cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca aataacgtcg
    13381 acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat ggttcttcaa
    13441 tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc aaagatggtc
    13501 gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat gatgtcgata
    13561 ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg agagcggagt
    13621 ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt aagtgagaat
    13681 aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac acctgccaga
    13741 agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt ttatgctggt
    13801 agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg cgggtcatat
    13861 gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt cacgtcgtcc
    13921 tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg cgtttgtgaa
    13981 tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag aacgcttccg
    14041 cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg ggttgctggc
    14101 aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc tcaacgtacg
    14161 agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta ttattgccgc
    14221 gttgattatt atctggactg gctggacacc tgctgacccc attctctcga tactggtgtc
    14281 gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg aattacttga
    14341 aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc gggaaatccc
    14401 ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc cggtgatgac
    14461 gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc agatccaaca
    14521 ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat atcaaccttg
    14581 tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc atcaccatta
    14641 atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc ggctgccgtt
    14701 gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc cctgaagtgc
    14761 aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt ccacatactt
    14821 acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat cccagaacgg
    14881 gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt gcatgatcat
    14941 gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca tcagaccaat
    15001 cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc agcgaatttt
    15061 caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt aaatattcgc
    15121 ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa agaaaataat
    15181 gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct ccagactggc
    15241 aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg agagatcata
    15301 accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa aatccatctt
    15361 tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta gccgcaaaat
    15421 ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg acatgaacct
    15481 cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca ttcatggcca
    15541 tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt ggtgcttcca
    15601 gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga tcggttgcca
    15661 caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg gtggaaccga
    15721 ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt atggcagcat
    15781 ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca tgcacaatgc
    15841 aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa cgccccagat
    15901 gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca ataagttcga
    15961 cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca gaagtgttgg
    16021 cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat tcttcaacag
    16081 ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt gttttttccg
    16141 gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac aaagtagaag
    16201 cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag ccaccggttt
    16261 cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc accgcattac
    16321 gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca atgcttaacg
    16381 gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt acgctcatct
    16441 taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat tcgctgttat
    16501 cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa agtcactcga
    16561 attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac gatgtcatca
    16621 ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta gggcgctttt
    16681 ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct
    16741 ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc gccgaccccc
    16801 tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg ctggatacta
    16861 caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat tcgaacctgc
    16921 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg tggtgggtga
    16981 tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa
    17041 tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg tgcaggatga
    17101 ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt cacgttttac
    17161 taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg accaattgat
    17221 taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc tttcggtttg
    17281 tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag cgcaacaaaa
    17341 atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga aataatgcga
    17401 cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct gtttagcgcc
    17461 atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt ttgcggtgtc
    17521 acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct ttggtgactt
    17581 cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt tacccttgtt
    17641 gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat cagggtaatt
    17701 tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat cctgcaccag
    17761 cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg cgccagcatt
    17821 cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt gatcgctgct
    17881 ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca ggatctgctt
    17941 ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac cacgcaggga
    18001 atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa gctggctgtg
    18061 agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg agctgacact
    18121 actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca gtaacgacag
    18181 actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa ccagtaccgc
    18241 acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt tttctttacc
    18301 gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt acatcttaac
    18361 ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac gttcgtccgc
    18421 gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg catgtgcatc
    18481 cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt aaacgatgtt
    18541 gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca tgttgccgtt
    18601 gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc cgtcattgct
    18661 ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa tcatcagccc
    18721 tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt aattattatc
    18781 acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga agacgcgctt
    18841 tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga gaagagctgt
    18901 agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac gtggacgaca
    18961 gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc ccaccattgg
    19021 agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg ttctgcgaac
    19081 cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa acctgcggac
    19141 gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag gtcggttcgg
    19201 tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa tccattacgt
    19261 acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg tctggcgaga
    19321 atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg ccatttgcca
    19381 gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc agtttagaac
    19441 cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg acaaactggt
    19501 tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg ttggtctgaa
    19561 caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt tcaaacactt
    19621 catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg tacgagttct
    19681 gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca acattgtaag
    19741 aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc agtgcggacc
    19801 atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga gcgcgatcta
    19861 acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca atatcttcag
    19921 gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga ccggaatcta
    19981 caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac agtatgagaa
    20041 aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga ccgatggccc
    20101 acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga accccgtggc
    20161 aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt ttgaagtcca
    20221 atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg atcttcgcaa
    20281 gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca ggtttgatat
    20341 ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg cctgcatagg
    20401 acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca tagttattga
    20461 tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc gaagcattgt
    20521 tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc tcaccgaaaa
    20581 tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct gcttttgccg
    20641 ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca gctgctgcct
    20701 tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct gctgccgctt
    20761 tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc ttggcttttt
    20821 cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct gccgcttccg
    20881 ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg gctgcggctt
    20941 tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc gctgcttctt
    21001 ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc ttctgcttta
    21061 actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc gctaaccgct
    21121 ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc agttcttcag
    21181 cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt gattcctggc
    21241 tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca gcgtcgatgg
    21301 acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa ctccagatca
    21361 gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc ttgagcttgt
    21421 cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc tgttacccgc
    21481 tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa accaaccgat
    21541 ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta aggcacatct
    21601 tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct ggacacttcc
    21661 gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac cacggtgtac
    21721 tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct caccgcctgt
    21781 gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg cgctgtcgcc
    21841 ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt gatttcggac
    21901 ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt gttgctctcg
    21961 ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa gttgtcgtaa
    22021 ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac cgccggaatt
    22081 gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg cgcaaccatt
    22141 tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat cccccagacc
    22201 gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa cggaatgtgc
    22261 gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga cgccccttcc
    22321 actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc tttgaacccg
    22381 ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact ctcttgatag
    22441 aggcgagaga gttcgattcc agaccagaat ttatcttcaa acgcttcggc ttcacgcgcc
    22501 gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat tgaaaaacca
    22561 atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa atcaaggata
    22621 ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca cgaggcttca
    22681 ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa gtattctcgg
    22741 cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt atttcagtct
    22801 gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc attttacgta
    22861 ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga tgacgcagca
    22921 tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc acaccaccgg
    22981 catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta ttcactttac
    23041 atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg cgcgcgattg
    23101 aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta gagcggccca
    23161 taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga caatgagaac
    23221 aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg gacgaaagcc
    23281 cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat gccagatttc
    23341 cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc ctgctaacag
    23401 aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg catacaaggt
    23461 cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc gacgtgttca
    23521 agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag aatccatgcg
    23581 aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt tcaatatctt
    23641 ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc agaatgatcg
    23701 gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg gaagttgcat
    23761 cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac gggaacattg
    23821 cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca aacgcccacg
    23881 cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca cccagtgccg
    23941 gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca gcttcacgaa
    24001 ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat ttcacgacat
    24061 aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc agtgtcacca
    24121 gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg gtacgcattt
    24181 gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct gccagcaggc
    24241 cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga tattcatcaa
    24301 cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc agcggcggaa
    24361 cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt tcttcaatct
    24421 tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg aggatcatcg
    24481 ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca aagagtgcgc
    24541 cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg gagttaatca
    24601 taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc acccccatgt
    24661 cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc caccagataa
    24721 aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc ggctgagtag
    24781 tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg ccgaggcgtg
    24841 caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg ccgcaaatca
    24901 gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca gctgtcggca
    24961 gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag cccgcttcta
    25021 cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat tttttctcgc
    25081 caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc agcaggaagc
    25141 cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg atggagtctt
    25201 tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta tagcgtttca
    25261 gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga accgtctggt
    25321 cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg ttacgaatgc
    25381 gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc acggaacgcg
    25441 ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc tcctcttcct
    25501 gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc cactcggctt
    25561 caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat tcatcaccca
    25621 gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca aaggagcgtt
    25681 tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg aggatgaaca
    25741 tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga gcaaccgggt
    25801 taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag ttgaaatcgg
    25861 acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg gacaggtttg
    25921 aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga cgatcccaac
    25981 cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga ccttcgattg
    26041 ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag ttagtcccga
    26101 actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata ccaaacaact
    26161 tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg tagaccgttt
    26221 ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg aagtggtaca
    26281 tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc atgactcctt
    26341 gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac gcatgcccca
    26401 atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa acggcgtaaa
    26461 gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata ttacgccgca
    26521 aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga cggtgatcaa
    26581 tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat ctctttttga
    26641 tgcccatttc caagacttat acattgataa atatcaattt ctacaaatta tgttaatcat
    26701 gtttcaacaa ggtaacttta gtgatattta tgtttaaaat gtgtgaatta taagactgaa
    26761 attagtttcg gttggcaatt aaagataaaa agagacgaag caaaaacagc acggttgacc
    26821 ttaattcttt ttttggtcac tggctaatac gacgcagagt ttcaggatta ttaataaagg
    26881 cataaatctc cctcccgccg atattaacat ttaattaact tataattata ttataattaa
    26941 ctcacccgtg gttattattt tgttaaaaat aaagccacat atacgtagct tgagagtgaa
    27001 gataaacaca aaattacctg atgcgctacg cttgccagtg tctacatcat ctctgcaata
    27061 tattgaattt gcctgctttt gtagaccgaa taaagcgttc atgccgtatc cggcatgaac
    27121 aaagcgccct ttgtcaacaa tctggccgcg catatgtggc ttcggtttga ttgctattca
    27181 ggcaagccgg taactgaacg tccgtgactg gccgggtaaa aaaggccccg atagatttga
    27241 accttgattt tcctcggtgg taatgtgttc atccatcatc gtttctgagc aagaaatcac
    27301 ctcgcgactg aaagcaacag tcgcatcaca ggttgctgat tcagccggat taaacagccg
    27361 taaaatcact tcttgtcggt cttcagcttt cttaagtgcg cttatcaggc atcccactgg
    27421 gggcattttc aacaaactat aactttccgg cacgttgaat ccggctttgt tgagcttcat
    27481 cgcatcccat gggattttgt tgtaacactg tactggagtc agccatgctc gcgcctgctg
    27541 agctacacca gcggcggttg gcgtaccggt ataactcaat aaacttaggc gacaagaaag
    27601 cagaccacgt agttgtgagt ccgggactgg cattttaatt cccgaaggcc gcctaggcct
    27661 taaaagcaga tcttctttcc ccagtaagcc cacgccgcgc agcaacgtaa tggcaaaggt
    27721 tttcttctct tcaccgatga cttcaaattc acgtagccct tcgctaaaga cagccatgcc
    27781 gttacgccct tcctgtaagg ctgcatagtt gagcatattc cataccggaa ccggcgcttc
    27841 tttccagctt tcttgctgcc agttgttcat tgcactgtcg ttcacggggc gcgttagcga
    27901 accaaactgg gtatctgcca gaacactatc ggtgttaaaa gatgtaggga tcaggacgcg
    27961 aaggcgatga tcgtcagcct ggttatcaag gttgatatcc acatcaatgc gcctgttgtt
    28021 atgactaaga gtgacaacca actcgacatc tactctgcca gtggattgtc ttgcgctgcg
    28081 ttgttgcaga ttacgcggca ccgccatttc atagcggata acagccctgc tctgccaggc
    28141 ctcatgaata atctcgcatt gcggtttcgc attcgccgaa gtaattaccc actcttcttt
    28201 tgcgggtgaa tagtcatatt catcaccatc atcagagctt tcttcaattt gcaacacccg
    28261 gtcatagcgc acaccgctat ctttatctac cagttgtagc gttccatcct cattgagcgc
    28321 aatttgccag aaagcatttt ccagtatccc ttcagcgtca ctttttgccg caatcacgtt
    28381 gccaggctga ttcgcttcga tataaagcgt gcgatagccc atagaaggga caatctggtt
    28441 gatctgtata tcaaactcca taaagggatc gtaattaccg taatgaacta tttgccgatc
    28501 gattaggcct ggatcgatct cacgggcatg gcgaataaaa tacggtacag gctgaccgcg
    28561 atcgtctcgt agattaaact ggctggcgcg cagccgcaca gtggtgttga tcacttcttc
    28621 ccgcggccag ggcatcaggt taaacaggac gagtttgtcg gcgtcgctct gcggcatgtt
    28681 gtcggcgatt ttacgcatat agaaacgcag cagattatcc gccatgtctt cagccagttc
    28741 gaagcgggcg acgatttcgc gatgaacttt gtcacgacag cagcagccga tgctgtcgtg
    28801 ggcatgattt tttaagatct ctttccacat tttctccagc aagccgtggt ggtatccaaa
    28861 acccaacgtc ctggccagcg ttgccagcgg ttccagcaga ttaacaatct tattttcaat
    28921 acgtgcgtgg gcaactttga tatccatgcg cgtggaaccg atggtgcgat gcacgcgcat
    28981 atatttgcca tcaataaatt cccctttcag ggttgccaga ttatctcgct gcgcttcgat
    29041 cttctcaaat acctcttcaa agcggctcat cacaaacttt cgttgaggat aaatttcgcg
    29101 cagtttctcc atcacttcga agatattttg ctgcaatggc atctgatcat gcccattcgg
    29161 cagcaaaatc tctttggtta cagacgcttt ttccagcacg tcaaaataac tgtcgaggcg
    29221 tttgcgtaat ccgttttcgt ccgcaggtaa gtacttaccg atggcataac ccaacggcat
    29281 cacctgcgcc gtcacttcgc taccgtcact gctttgccac aaaaactcgg ttttatccgt
    29341 accgtggcgc tcagaacatc cacgccagaa catggtgcag gtaatgccaa atccattgta
    29401 gatatgcggc agttgcccgg acatgccaaa ggaatcgggt aaataaccta ttttcatcgg
    29461 ctcgccaaac gcgaggcagt cacgcattcc gtacatcaga ttacggacga tggattccgc
    29521 agaaacaatc gtggtatcgg tctgggtata ccaggggccg ataatcaact tgccggcttc
    29581 taccagtttc ttcaagcggt ctttgttttc cggtttcacc gcgaaataat cttcgaggat
    29641 cgccgtttgc ccgtcgagta cgtaatattt gtattcgttg tcctgttcca gtcggcacag
    29701 gatctcttcc atattattga ccagcagaat gcgtgactct tcggtggtga aataccactc
    29761 ccgatcccag tgcatatgcg gggtgatgtg aacgcgagat accgctttca tcgtcgtttc
    29821 ctttttgttt atggcattac gccatcagtc agatagttgc catgcttaac cgcgtgacgc
    29881 cgccagatca ggagaattgc agtcgagatt gcagccccca ccaatgccgc accaaaccag
    29941 ccaattgccg ccataacacc gcccgcgcca ttatcatgaa gtaaaaagag cgagaaaatg
    30001 ccagcaccgg gtgtcgaaag gccgatattc atcgcaccga caatagcgcc cgttaccata
    30061 gagcccagca caaacgaacc aataacccgc agcggatctt caatcgccat cgggatcgcc
    30121 ccttcggtaa tacctgccag ccctaacagc caggtggatt tcccggtctc aatttcaaac
    30181 tctttaaaca ggcgcggtgc gagcatcgtg gaagcggtta cggtaaatgc cgaaaccatt
    30241 ttgacggagg cgaaaatggc atacgggccg taaacgccgt tcgccattgc gcccaggcag
    30301 aatgcataag cggctttatt cactggccct ccaaggtcaa aggaacacat aaaaccgaga
    30361 atggcaccca gcaacagcgc gttacttcct gacagaccgt tcagccaggc ggtaagcgag
    30421 ttattgatcc aggcgacagg ttcccccacc acaaacagca tcagactgcc cgctcccaat
    30481 gtaccgagca ccgggtagag ataaaaagtc aggaagccat tgaatttact gctaagacgc
    30541 aagtgatttt tcatccagcg tatcaagtaa ccggctatca atccgccaac gaccgcgccg
    30601 agaaacccgg agccgatcat gttggcggca agtccagccg caaagcctgg cgctaacgcc
    30661 ggtttatctg ccagcgaata ggcggtatag gctgccagca ccggtaccat caaaattccg
    30721 agcagcccgc cgcccagttt gcggtacatc cacagccagg agttttcttc attaaacaga
    30781 tcttgtagcc cgaagatttg cgacagtaat accgcgaccg ccagcaccgt cccccccgcg
    30841 acaatcaacg gtacggcaaa agagatcccg ctcaacagtg cctgtttcag ctccgttttg
    30901 acgcttttca ccggttgcgt atcttgctgt aaggtacgcg tctcatcgcc acgctcgagg
    30961 gtaagcgatt gttggatcaa cgcttctgca tggcgaatcg gctcggcaac aggcacagaa
    31021 agcgcaggaa tgccgttaaa acgctcactc tccttgatgg cgacttcagc cgcaaaaata
    31081 caggcagttg cactattgag ttgatccgcc gttaaacgcc cttcaatacc gttagcacct
    31141 tgcttttcaa cgtagacatt cacgccgagt ttgcgtccgg ctttttccag atactccgca
    31201 gccatgtagg tgtgagcaat acccgccggg caggccgtta cgcaaacgat agttggcgcg
    31261 ttggaaaaag aggcagaagg ttgcgtgcct cccttgtcat ccagcgccga gagcaattca
    31321 tcaggcgtcg tcgccgactg tatacgcgcc cgaatctcat catccgccag gcgcgtggtc
    31381 agcgctgtca gcagttgcat gtgcgtagta cccgcttcat tgaggggaat cgccagcaac
    31441 accactaaat caaccgcttc cgggccatcc acgccttccc actgaagcgg ctcgctgagt
    31501 gtggcgaccg caaacgccgc ttcttttacc gcagcagttt tgccatgcgg cacagccaac
    31561 ccttcactta aggcggtcgg gccaaggctt tcacggcgat acacttcttt cagaaattgc
    31621 tcagtactgg aaattttccc cagagcagca agacgttgag tcaacgcgtg aatggcctct
    31681 tcacggctgg taaagcgcgc attcagacac aacgcatcgc ggtgggtcag agtcgtcagg
    31741 ttcatcatga tcctcacttg atcgttttta tagtcgcgcc agtgtgcccg acaaaacaat
    31801 accattgcgt aatcaatttc acttgattga aattatttgc attttatttg tattgtttac
    31861 aggatgagtt tatcgggcat aatggctaaa ttcgtattaa tgagatgcta aataatggga
    31921 cacaaaccct tataccgaca gattgctgat cgcattcgtg agcaaattgc gcgtggcgag
    31981 cttaaacctg gcgatgcgtt acctaccgaa tcggcattac aaacagagtt tggcgtcagc
    32041 cgggttacgg tgcgtcaggc tttgcgccag ttagtcgagc aacagatcct cgaaagcatt
    32101 cagggcagcg ggacttacgt caaagaagag cgggtcaatt acgatatttt tcagttaacc
    32161 agttttgatg aaaaactgtc ggaccgtcac gtcgatacac acagtgaagt tctgatattt
    32221 gaagtgattc cggctgacga ttttcttcag caacagctac aaatcaccgt gcaggatcgt
    32281 gtttggcatg tgaagcgcgt gcgctatcgc aagcaaaagc caatggcgct ggaagaaacc
    32341 tggatgccgc ttgcgttgtt cccggatctc acctggcagg tcatggaaaa ttcgaaatat
    32401 cactttattg aagaagtgaa gaagatggtt atcgatcgta gcgaacagga aatcattcct
    32461 ctgatgccaa ccgaagagat gagccgtctg cttaatatca gccagacaaa gccgattctt
    32521 gaaaaagtat cgcgtggata tttagttgat ggtcgggtgt ttgaatatag ccgcaacgct
    32581 tttaataccg atgactataa atttaccctg atagcgcagc gaaaatcatc gcgataagca
    32641 caaaaaggcc atcatacgat ggccaaccat gtcgaaaccg acagcccttc gccattcctg
    32701 tagagggaag cggcgagggc tatttcttat tacagatatt tatttcagaa cagttttcag
    32761 tgcttcaccg atatccgcca ggctgcgaac ggttttcacg cctgcggctt ccagagcagc
    32821 gaatttctca tccgcagtcc ctttcccacc ggcaatgatc gcacccgcgt ggcccatacg
    32881 tttgcctttc ggcgcagtca caccagcgat gtaacccaca actggcttgg taacatgctc
    32941 tttgatgtac gctgccgctt cttcttcagc gctaccgccg atctcaccga tcatcacgat
    33001 cgcttcggtc tgcggatctt tttcgaacat ttcgagaatg tcgataaagt tagagccagg
    33061 gatcgggtca ccgccgatac cgacacaggt cgactgaccg aaaccgtaat ccgtggtctg
    33121 tttaaccgct tcataggtca gtgtaccgga acgggaaacg atacccactt tacccggttt
    33181 gtgaatgtga cccggctgga taccaatttt gcattcaccc ggggtgataa cgcctgggca
    33241 gttcgggccg atcatacgaa cgcctgcttc gtccagtttc actttcacgg tcagcatatc
    33301 cagcgtcggg atgccttcag tgatggtgat aatcagtttg atgcctgcat cgatagcttc
    33361 cagaatggag tctttgcaga acggtgctgg tacgtaaata acagacgcgg aagcgccagt
    33421 agcagcaaca gcttcacgca cggtgttgaa caccggcagg ccgaggtggg tagtgccgcc
    33481 tttacctgga gttacgccac caaccatttt agtgccgtat gcaatggcct gttctgagtg
    33541 gaaagtcccc tggctaccgg taaagccctg gcagataacc ttggtgtttt tatcaattaa
    33601 aatggacatt atttcccctc cactgcggca acaacctgct gagctgcatc cgtcagacct
    33661 tttgctgcaa taatattcag gccgctgtca gccagtttct tcgcgccgag ttcggcgttg
    33721 ttaccttcca gacgtaccac gaccggtacg ttaacaccca cttccgctac cgcgccgatg
    33781 ataccgtcag cgatcaggtc gcaacgaacg ataccgccga agatgttaac cagaacggct
    33841 ttcactttgt cgtcagagag gatgattttg aacgcttcgg ttacacgttc tttggttgcg
    33901 ccgccgccaa cgtccaggaa gttagccggt tcgccgccgt gcagtttaac tatgtccatc
    33961 gtacccatcg ccagacctgc gccgttaacc atacaaccga tgttaccgtc cagcgcaacg
    34021 tagttcagtt cccactgtgc agcctgtgct tcacgcggat cttcctgcga ctggtcacgc
    34081 atttcgcgca gatcaggctg gcggaacagg gcgttgccat cagcgcccag tttgccgtcg
    34141 aggcaaatca gatcgccctg tttggtgatg accagcgggt tgatttcgat cagcgccagg
    34201 tcgcgctcca ggaaaatggt cgccaggccc atgaagattt tggtgaactg ctgaaccagt
    34261 ttaccttcca gacccagttt gaacgccagc tcgcgtccct gatacggcat cgggccagtc
    34321 agcggatcaa gcgcaacttt atggatcagg tgcggagttt cttccgccac tttttcgatt
    34381 tccacgccgc cttcggtgga ggccataaag accacgcgac gggaactacg gtctacaacg
    34441 gcaccgagat acagctcttt agcgatatcg gtcgccgctt caaccagaat ctggttaacc
    34501 ggttggccat tggcatctgt ttgatacgtt accagacgct tgcccagcca gttttctgca
    34561 aaagcacgga tgtcttcttt gctgtttaca actttcacac cgcccgcttt accgcggcca
    34621 ccagcgtgaa cctgacattt cactacccac ggaccggcac cgatttttga agcggcttct
    34681 tctgcttcgc gcggagtagt acaggcataa cccaccggtg ctggtaagcc atagcgggca
    34741 aacagttgtt ttgcctgata ttcatgtaag ttcatgtgtt ctgtccatcc ttcagtaatc
    34801 gttatctttt aaaccgtaga ccgacaccct atccacgtcg gtctacagtg caggtgaagc
    34861 ttaaactact acacgtccag cagcagacgc gtcggctctt ccagtaactc tttgatcgtt
    34921 accaggaagc ccacggattc gcgaccatcg atcagacggt gatcgtagga cagcgccagg
    34981 tacatcatcg gcaggatctc aacctgacca ttcaccgcca tcggacgatc tttgatagcg
    35041 tgcataccca gaattgcgct ctgcggcggg ttgatgatcg gcgtagacat cagggaaccg
    35101 aacacaccac cgttggtgat ggtgaagtta ccaccggtca gatcttcaac cgtcagcttg
    35161 ccatcacggc ctttgactgc cagctctttg attttcttct cgatgtctgc catgccgagg
    35221 gtatcgacat cacgcagaac cggcgtcacc aggccgcgcg gcgtagaaac cgccatgctg
    35281 acgtcgaaat agttgtggta aaccacgtca tcgccgtcga tagacgcgtt cacttccggg
    35341 taacgtttca gggcttcaac caccgctttc acgtagaagg acataaagcc cagacggatg
    35401 ccgtggcgtt tttcaaacgc ttcaccgtac tgcttacgca gatccataat cggcttcatg
    35461 ttgacttcgt tgaacgtggt cagcatggcg gtggagtttt tcgcttccag cagacgctct
    35521 gccacacgct tacgcaggcg agtcatcggg acacgttttt cactacgtgc agccagagcc
    35581 ggttgcgccg ccggagcagc cgctgccgga gcagactctt tcgccggggc tttcgccaga
    35641 tgtttttcca catcttcacg agtcagacga ccacccacac cggtgccttt aatggcgctg
    35701 gcgtcgagat tgtgttcagc cagcaggcga cggatcgccg ggcttaacgc atcgttgttt
    35761 tgctcttcca gagacgcctg ctggcgttgc gccggagtgg acgctttctc ttcagatttg
    35821 gcgctggttt ctttaccggc gctgttgcct tcacgcaggc gaccaaggat ctgacgagac
    35881 gttaccgttg taccttcatc ttccagaacc gcatccagaa tgccgtctgc tgatgccggt
    35941 acttccagta ccactttgtc agtttcgatt tctaccagca cttcatcacg tacgactgcg
    36001 tcgccgggtt ttttatgcca ggttgcgacg gtggcatcgg ctacggattc aggcaggtca
    36061 gggaccagaa tatctacgct actcattgtg tatcctttat ttattcgacg ttcagcgcgt
    36121 cattaaccag atcttgttgc tgtttctggt gaacggacat ataccctacc gccggagagg
    36181 cggaggccgg gcggcctgca taacgcagag aagccccaaa cggaatcact tcacggaaat
    36241 gatgctggct gcagtaccat gcgccctggt tgagcggctc ttcctggcac cagacaaaat
    36301 ccttgacgtg agcaaactgc tgcaacactt cctgcatcgc tttatgcggg aacgggtaga
    36361 gttgctcgat acgcacaatg gcgacatcgt gttgattgtt cttacgacgc tgttccagca
    36421 ggtcgtaata aaccttacca gaacacatca ctacgcgctt cacgcccttc ggatcaagct
    36481 cgtcgatttc accgatggct ggcaggaagg tgccgttcgc cagttcttcg aggctggaaa
    36541 ccgccagcgg atgacgcagc agggatttcg gcgacatcac gaccagcgga cgacgcatcc
    36601 cgcgcagcgc ctgacgacgc agcatgtggt aaacctgtgc cggggtagac ggtacgcaaa
    36661 cctgcatgtt ttgctcagca caaagttgca gataacgttc cagacgcgcg gaggagtgct
    36721 ccggcccctg cccttcgtaa ccgtgcggca gcaacatcac cagaccacac atccggcccc
    36781 atttctgctc gccagaggag atgaactggt cgataaccac ctgcgcaccg ttggcgaagt
    36841 caccgaactg cgcttcccag atggtcaggg tgcgtggttc tgcggtggca taaccatatt
    36901 caaacgccag cacggcttct tctgacagta cggagtccca gacacggaac gcgccctgac
    36961 cgttatggat gtgttgcagc ggcgtgtaag tggaaccgtt agactggttg tggatcaccg
    37021 cgtggcggtg gaagaaggta ccgcgaccgg agtcttcacc cgacaggcga accggaatgc
    37081 cttcatcaac cagcgtggcg taagcgaggt tttccgcacc gccccagtcg aacagtttct
    37141 caccggctgc catcgcctgg cgatcgccat aaatcttggc aacgcgagac tgcatttcaa
    37201 ctgcttctgg taccgtactg atacgtttag ccagttcctg caggcgcttc atctcaactt
    37261 tgttcgggta ctcttcgtcc cattcgtggt tgaggtacgg cgaccaggtg aaagagtgca
    37321 tgttcatcgg acgccactct gccactacgc aatcgccagc atccagcgca tcgcggtaca
    37381 ggttaaccat ctcggtggca tcttccagcg tcgccacttt ttcctgctcc agcttgtcag
    37441 cgtagatttt gcgcggtgtc ggatgttttt tgattttctg atacatcagt ggctgggttg
    37501 cgctcggctc gtcggcttcg ttgtggccgt gacggcggta gcataccagg tcgatgaaga
    37561 catcacgttt aaaggtgtta cggaaatcga gcgccagacg ggtcacaaag gcaacggctt
    37621 ccggatcatc cgcgttaacg tggaaaatcg gtgcctgaac catcttaccg atatcagtac
    37681 agtacggtgt agaacgggca tccagcgggt tagaggtggt gaaaccaacc tggttgttga
    37741 taacgatacg taccgtaccg ccaacttcat aaccacgcgc tttcgacatg ttcagagttt
    37801 cctgaaccac gccctgcccg gtcactgcgg cgtcaccgtg gatggtgatt ggcagcactt
    37861 tgttgctgtt cggctcatca agtctgtcca gacgggcacg aacagagccg ataacgaccg
    37921 ggcttacaat ctcaaggtga gacgggttaa acgccagcgc caggtgcacc aggccgccat
    37981 cggtctggaa gtcagacgag aagcccatgt ggtatttcac gtcgcctgtg ccgaggtgtt
    38041 ctttatgttt accggcgaac tcgtcgaaca agtcttgcgg ttttttaccc agcacgttca
    38101 ccagcacgtt cagacgacca cggtgcgcca tcccgagaac cacttcgcgg gtgccgctgt
    38161 tgccagcgtg gcggatcatc tctttaagca tcgggattaa cgcgtcaccg ccttccagcg
    38221 agaagcgttt tgcgccaggg aattttgcgc cgaggtaacg ctcaaggcct tcagcggcgg
    38281 tcagttcgct taagaagcgt tttttctctt cgctattgaa agtcgcgcga ccagactcga
    38341 tacgctgttg gatccagcgt ttttcttcgg tgctggtgat gtgcatatac tcggcaccaa
    38401 tcgggccgca gtaggtttgc ttgagggcgg aaataagctc gccgagtttc atggtttctt
    38461 tgccgctggc aaatgaaccg acgttgaagg tttcctggaa gtctgcttcg gtcagatcgt
    38521 ggaaagacgg atccagatcg gccactttat cttgctgcca cagtcccagc ggatctagat
    38581 tcgcatgctg gtgaccacgg aagcggtatg cgttaatgag ctgcaggact ttaacctgct
    38641 tcacattggt gtcagggtcg gagatcgttg aagagtaacg tgaagcgtct ttcgccaggc
    38701 ggcggaaata ttcacgcgtt tgagagtgga attgatccgg tttgactccc gtaccaggta
    38761 actgctggaa cgtcgaacgc cagttagcgt caaccgagtc aggatcggtt aagaagtctt
    38821 catagagctg ttctatccag ctctggtttg cgccagagag gtaagaagag tccaaccagg
    38881 ctttcaaagc gctgttctgc atcgtgatcc cttaagcatc ttttttatgc ttacttcgcc
    38941 gtggatacta ccacgcacag tgatgtgcgt gccggggttc acttgcgagc tcttgtctac
    39001 gtagtggctc gcgaaggaac ctttaaaaac tgtcttagtg tcaggggcag tttttaaaga
    39061 tttcctgcat ttctttgtta cgcctgatgc gctgcgctta tcaggcctgc gggtaatgac
    39121 ttgtaggccg gataaggcgt tcacgccgca tccggcactg gttgcctgat gcaacgcttg
    39181 cgcgtcttat caggcctaca gtttacgcat tacgttgcaa caacatcgac ttgatatggc
    39241 cgatggcgcg cgtcgggttc agccccttcg gacatacact gacgcagttc atgatgctgt
    39301 gacagcggaa tacgctgaat gcatcgctca aaccgtcgag gcggctgtca gtctcggtat
    39361 cacggctatc gatcaggaaa cgatatgccg ctaacaagcc tgccgggccg ataaacttat
    39421 cgggattcca ccagaaagac ggacaagagg ttgaacaaca tgcgcagaga atacattcat
    39481 acagcccgtc gagtttttcg cgctgctctg gcatctgtaa atgctcgcga gctggcggat
    39541 tttgtccatt attcaacagg taaggcttaa ttttctcata ttgcgcatag aattgtccca
    39601 tgtctaccac caaatcgcgg atcaccggta aacctggcag cgggcgaatc acaatcttct
    39661 tgcccggctg gttgagtgcc gaaatcgggg taatacaggc cagaccattc ttgccgttca
    39721 tgttcagacc gtcggaaccg cacacacctt cacggcagga gcggcggaac gacaggctgg
    39781 gatctttctc tttcagctgg ataagcgcat ccagcagcat catgtcgcga ccttcttccg
    39841 cttccagggt gtaatcctgc atacgcggag catcatcaac atccgggtta tagcgataaa
    39901 ttgaaaactc gagtctcatt ttcctgtctc cgcattagta agtacgaatc ttcggcggga
    39961 atgccgggcg cagtttcggt tccatgttga cgcttcggcg cgtcatggat tccgactcag
    40021 gcagatacag ggagtggcac agccagtttt catcatcacg atccgggaag tcgaagcggc
    40081 tatgtgcgcc acggctttcg gtacggaagt tggcagaaac agccgttgca tacgccgttt
    40141 ccatcaggtt atccagttcc aggcactcaa cgcgctgggt gttgaactcg ctggaagtgt
    40201 catccagacg ggcatttttc agacgctcgc ggatcacttt caactgctca agccctttcg
    40261 ccatcgcatc accttcacgg aagaccgaga agttatgctg catacattct tgcagcgctt
    40321 tacggatcgc caccggatct tcaccgttac gattattgtt ccaccggttc aggcgatcca
    40381 gagacgcttc aacatcagac tcgctggcat cgtgcagtgc gccctgctcg gcgatagact
    40441 cttgcagatg cagacctgcc gcgcgaccaa agaccaccag gtccagcagc gagttgccgc
    40501 ccagacggtt agcgccgtgt accgatacac aagcgatttc accaacggca aacagtcccg
    40561 gaacaaccac atcttcgcct ttctcattca cagtcagcgc ctgaccggta actttggtcg
    40621 gaataccgcc catcatgtag tgacaggttg ggataaccgg aatcggctct ttcaccggat
    40681 cgacgtgagc gaaggtacgg gaaagctcca ggatacccgg cagacgggat tcgagaactt
    40741 ctttacccag gtgatcgagt ttcagtttcg cgtgtggccc ccacggacca tcacagccgc
    40801 gaccttcacg gatttcaatc atgatggaac gcgccaccac atcacggccc gccaggtctt
    40861 tggcgttcgg cgcataacgc tccataaagc gttcgccatg tttgttcagc agataaccgc
    40921 cttcaccacg gcaaccttcg gtgaccagta cgcccgcacc ggcaatgccg gtcgggtgga
    40981 attgccacat ttccatatcc tgcaccggta cgcctgcgcg gatagccatg ccgacaccgt
    41041 cgccagtgtt aatgtgggcg ttagtggtgg actgataaat acgccctgct ccgccagtcg
    41101 ccagcacggt agcgcgggct ttgaaataaa ccacttcacc ggtttcgatg cacagtgcgg
    41161 tacaacccac caccgcgcca tcctggtttt tcaccagatc cagcgcatac cactcggaga
    41221 aaatggtggt gtggtttttc aggttctgct gataaagcgt gtgcaacagt gcgtgaccgg
    41281 tacggtcagc agccgccgca gtgcgtgccg cctgctcgcc gccgaagttt ttcgactgac
    41341 cgccaaacgg acgttgatag atacgaccat catcgagacg cgagaacggc aggcccatgt
    41401 gttcgagttc cagaatcgct tccggcccgg ttttacacat atattcaatc gcgtcctggt
    41461 caccgatata gtccgaccct tttacggtgt cgtacatatg ccattcccag ttatcttcat
    41521 gggtattacc cagcgcaacg gtaatgccgc cttgcgcaga aacggtatgg gaacgggtcg
    41581 ggaagacttt agagagcagg gcacaggtct ggccgctctg ggaaatttgc agcgccgcgc
    41641 gcatacctgc gccaccggca ccaatcacaa ctgcatcaaa ttctctgact ggcaatttca
    41701 tcacacaccc cacaccacaa cgaatccata aatcacgtaa accaccagtg caacgacaat
    41761 caccagttgc agcatcaggc gcaaggccag cggtttaacg tagtcggtca acacctgcca
    41821 catgccgatc caggcatgaa tcaagataga aaacagcgcc agcagggtga acactttggt
    41881 gaacgcagag gcgaagaaac cgatccagac ttcatatgtc agttcgccac tggtggcgaa
    41941 aaaaccgacc atataaatga tgtagagcgt caggacgata gcggtagcgc gaacgaggat
    42001 gaaatcatgt acgccattgc gtcctaatgc ggaggcgttg cttaccatac gaggactcct
    42061 gcgagaagtg aaagcacgac agtaataaca aaggagattt tggcggagcg tttacccgct
    42121 tcgaatgttt cttccagata gccaaaatcc atcatcatgt ggcgaatacc tacgacgacg
    42181 tgatacgcca gagcggtaag gatgccccac atgataaatt tgacgaagaa gctgcccata
    42241 atcgcggaag cttgctcgaa accttcaggg gaagagaggc tggtacccag aagccactgc
    42301 aggatgccca ctgcaacaaa ggtgatcaca ccggaaacgc gatggagaat ggacgctatc
    42361 gccgtgatgg ggaaccggat ggtctgtagg tccagattaa caggtctttg ttttttcaca
    42421 tttcttatca tgaataacgc ccacatgctg ttcttattat tccctgggga ctacgggcac
    42481 agaggttaac tttctgttac ctggagacgt cgggatttcc ttcctccggt ctgcttgcgg
    42541 gtcagacagc gtcctttcca taactgcgcg tcatgcaaaa cactgcttcc agatgcgaaa
    42601 acgacacgtt acaacgctgg gtggctcggg attgcagggt gttccggaga cctggcggca
    42661 gtataggctg ttcacaaaat cattacaatt aacctacata tagtttgtcg ggttttatcc
    42721 tgaacagtga tccaggtcac gataacaaca tttatttaat ttttaatcat ctaatttgac
    42781 aatcattcaa caaagttgtt acaaacatta ccaggaaaag catataatgc gtaaaagtta
    42841 tgaagtcggt atttcaccta agattaactt atgtaacagt gtggaagtat tgaccaattc
    42901 attcgggaca gttattagtg gtagacaagt ttaataattc ggattgctaa gtacttgatt
    42961 cgccatttat tcgtcatcaa tggattcttt acctgcaagc gcccagagct ctgtacccag
    43021 gttttcccct ctttcacaga gcggcgagcc aaataacaaa cgggtaaagc caggttgatg
    43081 tgcgaaggca aatttaagtt ccggcagtct tacgcaataa ggcgctaagg agaccttaaa
    43141 tggctgatac aaaagcaaaa ctcaacctca acggggacac agctgttgaa ctggatgtgc
    43201 tgaaaggcac gctgggtcaa gatgttattg atatccgtac tctcggttca aaaggtgtgt
    43261 tcacctttga cccaggcttc acttcaaccg catcctgcga atctaaaatt acttttattg
    43321 atggtgatga aggtattttg ctgcaccgcg gtttcccgat cgatcagctg gcgaccgatt
    43381 ctaactacct agaagtttgt tacatcctgc tgaatggtga aaaaccgact caggaacagt
    43441 atgacgaatt taaaactacg gtgacccgtc ataccatgat ccacgagcag attacccgtc
    43501 tgttccacgc tttccgtcgc gactcacatc caatggcagt catgtgtggt attaccggcg
    43561 cgctggcggc gttctatcac gactcgctgg atgttaacaa tcctcgtcat cgtgaaattg
    43621 ccgcgttccg cctgctgtcg aaaatgccga ccatggccgc gatgtgttac aagtattcca
    43681 ttggtcagcc atttgtttat ccgcgcaacg atctctccta tgccggtaac ttcctgaata
    43741 tgatgttctc cacgccgtgc gaaccgtatg aagttaatcc gattctggaa cgtgctatgg
    43801 accgtattct gatcctgcac gctgaccatg aacagaacgc ctctacctcc accgtgcgta
    43861 ccgctggctc ttcgggggcg aacccgtttg cctgtatcgc agcaggtatt gcttcactgt
    43921 ggggacctgc gcacggtggt gctaacgaag cggcgctgaa aatgctggaa gaaattagct
    43981 ccgttaaaca cattccggaa tttgttcgtc gtgcgaaaga taaaaatgat tctttccgcc
    44041 tgatgggctt cggtcaccgc gtgtacaaaa attacgaccc gcgcgccacc gtaatgcgtg
    44101 aaacctgcca tgaagttctg aaagagctgg gcaccaaaga tgacctgctg gaagtggcta
    44161 tggagctgga aaacatcgcg ctgaacgacc cgtactttat cgagaagaaa ctgtacccga
    44221 acgtcgattt ctactctggt atcatcctga aagcgatggg tattccgtct tccatgttca
    44281 ccgtcatttt cgcaatggca cgtaccgttg gctggatcgc ccactggagc gaaatgcaca
    44341 gttacggtat gaagattgcc cgtccgcgtc agctgtatac aggatatgaa aaacgcgact
    44401 ttaaaagcga tatcaagcgt taatggttga ttgctaagtt gtaaatattt taacccgccg
    44461 ttcaaatggc gggttaattt ttatatgcct aaacacaaaa aattgtaaaa ataaaatcca
    44521 ctatcggaca ttcatatatt ctaaaaaaaa atacatcggt ataaattata aacaaataga
    44581 tgatttttaa tcatctatct tcctctcagt tacatgaata aaccaccaaa tatagcgcac
    44641 ccctataatt cccaatactt tattcatcaa acatttaatt cataccatca attaattact
    44701 tttacattta ataaaattaa taatattttt aagaaaaact aaaaacattc gacaatcaaa
    44761 taagatattc ttgcaccgat atactttttt cttttctgtt aaaggactca gttatgttta
    44821 aaggacaaaa aacattggcc gcactggccg tatctctgct gttcacagca cctgtttatg
    44881 cggctgatga aggatccggt gaaattcact ttaaaggtga agttattgaa gcaccgtgtg
    44941 aaatacatca ggatgatatt gataaagagg ttgaactcgg tcaggtgacc accagccaca
    45001 ttaatcagtc acatcacagc gatgccgttg ctgtcgacct gctcttagtc aactgtgatc
    45061 tggaaaactc cagcaacggt tccggtggca agatttctaa agttgcagtc acctttgata
    45121 gctcagcgaa aaccaccggc gcagatccga ttctcaacaa caccagcacg ggtgaagcca
    45181 ccggcgtcgg cgtacgttta atgaataaag atcaaagcaa tatcgtttta ggcaccgcta
    45241 ctccagatat cgacctggct ccgacctcca gcgaacaaac gctgaatttc tttgcctgga
    45301 tggaacaaat tgatcaggct acacctgtaa cgccaggcgc agttacggcg aatgcaacct
    45361 acgtgctgga ttataaataa gtattacttt ggcggcgcta tcgccgcctt tcataaaata
    45421 tttatcaggt atggacaccg tgaatattta tcgactctct tttgtatcct gcctggtcgt
    45481 ggcgatgcct tgcgcattgg cggtcgaatt caatctgaat gttctcgata aatcaatgcg
    45541 cgaccgcatt gatatttcat tattaaagga aaaaggagtc attgctcccg gtgagtattt
    45601 tgttagcgtt gcggtaaata ataaccaaat cagtaacgag caaaagatta actggcacaa
    45661 aaatgacgat aaaaccattc cgtgcatcaa tgatttactg gtcgataaat ttggcttaaa
    45721 acctgaagtc cgtcagtcgt taccattgat aaatcagtgc gtcgatttta gctcccgacc
    45781 tgaaatgctc ttcaatttcg atcaagccaa tcagcaactg aatattagta ttccgcaagc
    45841 ctggctggcg tggcactcag aaaactggac cccaccctcc acatggaaag aaggtgtcgc
    45901 cggtgtcctg atggattaca acttgtttgc cagcagctac cgcccacagg atggcagcag
    45961 cagcactaat cttaacgcct atggaacagc cggaatcaac acgggggcat ggcgcttacg
    46021 cagtgattat cagttgaatc atactgatag cgatgataac catgaacagt caggcgaaat
    46081 atcacgtacc tatctttttc gcccgttacc gcaattaggt tcaaaattaa ccctcggcga
    46141 aacggatttt agtcccaata ttttcgacgg tttttcttat accggcgcgg cactggcaag
    46201 tgacgatcga atgttgccat gggaactacg cggctacgcc ccacaaatta gcggtattgc
    46261 acagaccaat gccacggtga cgatcagtca atcaggccgc gtcatttacc agaaaaaagt
    46321 cccaccaggc ccatttatca ttgacgacct taatcagtct gttcagggca cactggatgt
    46381 caaagtgacg gaagaagatg gtcgggtgaa caatttccag gtttcagcag catcgacgcc
    46441 cttcctgact cgtcagggac aggttcgcta taaactggcc gcgggtcagc cacggccctc
    46501 catgtcacat caaactgaaa atgaaacctt ttttagcaat gaagtttcct gggggatgct
    46561 gtcaaacacc tcgctgtaca gcggcctgct gctttctggt gatgactacc attctgccgc
    46621 aatgggtatt gggcaaaata tgctgtggct tggtgcgctg tcgtttgatg tcacgtgggc
    46681 cagtagccat tttgatactc agcaggacga gcggggctta agctaccgtt ttaattacag
    46741 caaacaagtg gatgccacta acagcacgat ttccctcgcc gcttatcgtt tctccgatcg
    46801 tcattttcac agctacgcca actatctgga tcacaaatac aacgacagcg atgcgcagga
    46861 cgaaaaacag acgatcagct tatctgtggg ccaaccgatt accccactaa acctcaatct
    46921 ttacgccaac ctgctacatc aaacctggtg gaatgcagac gcctccacga ccgccaacat
    46981 cacggcaggt tttaatgttg atattggtga ctggagagat atctctattt ctacgtcatt
    47041 caatacaacc cattacgaag ataaagatcg cgacaaccag atttacctgt cgatttcgct
    47101 ccccttcggt aacggtggtc gggttggcta tgacatgcaa aacagtagcc acagcaccac
    47161 acaccgcatg tcgtggaacg atacgctgga tgaacgtaat agctggggca tgtctgccgg
    47221 actgcaatcc gaccgtcctg acaatggagc ccaggtgagc ggtaactatc agcacctgag
    47281 ttcagcgggt gagtgggata tttctggtac ctatgccgcc aatgattaca gttccgtcag
    47341 cagcagctgg agcggttctt tcaccgcaac ccaatatggt gcagcgtttc atcgccgcag
    47401 ctccaccaat gaaccacgcc tgatggtcag caccgatggc gtggcagata ttccggttca
    47461 gggcaatctc gactacacca accattttgg cattgcggtg gtgccgttga tttccagtta
    47521 ccagccttcc accgtggcgg tgaacatgaa tgacttaccc gacggcgtaa cagttgcaga
    47581 aaacgttatc aaagaaacgt ggattgaagg cgcgataggt tacaaatcac tggcttcccg
    47641 ttccggtaaa gacgttaacg tcatcattcg caacgccagc ggtcagtttc ctcccctcgg
    47701 agcggatatc cgccaggatg acagcggcat tagcgtgggg atggttggcg aggaaggaca
    47761 tgcctggtta agcggagtcg ctgaaaatca aaagtttacc gtggtctggg gtgatagcca
    47821 gcattgctcg ctccatcttc ctgaacatat ggaagacacc gcaaatcgcc tgattttacc
    47881 ttgtcattaa ttaaggaaat gaaaatgaca tttatgaaag gactacccct tttgttgttg
    47941 gttgccagtc tgtgcagcca cgctgcacta caacccgatc gcactcgtat tgtgtttaac
    48001 gctaatgata aagctaccag tctgcgggtg gataatcgta gcgataaact cccctacctc
    48061 gcttattcct ggctcgaaaa tgaaaaaggg gagaaaagcg atgatcttct ggtcgccctg
    48121 ccgccgatac aacgtctgga accgaaagca acaacacagg tacggatagt aaagcaagcc
    48181 tcgaccacaa aattaccggg cgatcgcgaa acgctatttt tctacaatat gcgtgaaatt
    48241 cctcccgccc cggaaaaaaa cagcgaccat gccgttctgc aagtcgccat tcagagtcgt
    48301 atcaaggtgt tctggcgacc ggctgcatta cgcaagaaag caggagaaaa ggtcgaactg
    48361 cagttacagg tcagccagca gggcaaccaa ctgacactga aaaatcctac cgcgtattat
    48421 ctgaccattg cctacctggg acgaaatgaa aaaggcgttc tccccggctt caaaaccgtg
    48481 atggttgcgc cttttagtac ggtcaacaca aacaccggaa attatagcgg cagccagttt
    48541 tatctcggtt atatggatga ttacggcgcg ttacgcatga cgacgctcaa ctgcagcgga
    48601 caatgccatt tacaagccgt ggaggcgaag aaatgagtgc tggcaaggga gtgttgctcg
    48661 tcatctgtct gttatttctg tcgttgaagt ctgcgcttgc cctgaactgc tactttggtt
    48721 catccggtgg ctcggtagaa aaatcagaag cgattcaacc ttttgctgta ccaggcaatg
    48781 ccaaactcgg cgataagatc tgggaatctg acgatattaa aataagatct gggaatctga
    48841 cgatattaaa attcccgtct attgtgacaa caataccaac ggcaactttg aaagcgaaca
    48901 tgtctacgta tgggtaaacc cctacccggg ggtgcaggat cgttattatc aactcggagt
    48961 aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg acactaacca
    49021 gtgtatcgac agtaagaaca ttgacattta tacccctgat gggatggcag aacaaaattt
    49081 gctcgggtga tcccgctaat attcacatgt cgcgaacctt tctcgctcgc atgcggttat
    49141 acgtcaaaat acgagaaatg ccgccgcatg attatcaaag tacgcttagc gactatatcg
    49201 tcgtgcaatt tgacggtgcc ggtagcgtta acgaagaccc tactgcccaa aacctgaaat
    49261 atcatattac tggtctggaa aacattcgcg tgctggattg cagcgtcaat ttttccattt
    49321 caccggaaac acagatgatt gattttggta aatttaattt gctggatatt cgtcgccaca
    49381 caatgtcgaa aacattcagt attaagacga caaaatcaca aaatgaccaa tgcaccgacg
    49441 gatttaaagt cagttcctcc ttttataccg aagaaacgtt ggttgaagaa gataaagcat
    49501 tgttgattgg taatggtctg aagctacgtt tgctggatga aaacgcctcg ccttatacct
    49561 tcaataaata tgctgaatac gccgatttca ccagcgacat gttggtctac gaaaaaacct
    49621 atacagctga actttcgtcc attgcaggta cccccatcga ggctgggccc ttcgatacag
    49681 tggtgctttt taaaattaac tataactgat ttgccatgat gccctttgca gccggaaagg
    49741 gcatctcttt cagaaaattg ctgattttcc ccttttcctt gattgctgtt attttttcct
    49801 agcccggctt tctttatgct gtatcgatac accaaagaga aaataatgag ggagcgaagg
    49861 atgccagttt tgcagtgggg aatgttatgt gtgctatcac ttctcctttc tattggtttc
    49921 ctcgcggtac atctcccggc ggcgctatta cttgggccga tgatcgccgg gatcatcttc
    49981 agtatgcgcg gaattactct gcaacttccc cgctccgctt ttctcgccgc gcaggccatt
    50041 cttggctgca tgattgcgca aaacctcact ggttcgattc tcaccacgct ggccgccaac
    50101 tggccgatcg tgcttgctat tttactggtg acgctgcttt ccagcgccat cgtgggttgg
    50161 ttattggtgc gctatagctc actgcctgga aataccggtg cctggggttc ttctccaggc
    50221 ggcgcagcgg caatggtcgc tatggcgcaa gattacggcg cagatattcg cctggtggcg
    50281 tttatgcaat atctgcgagt gctgttcgtc gcgggggccg ctgttctggt cacccgcatg
    50341 atgctgggtg ataacgctga agcggttaac cagcaaatcg tctggttccc gccagtgagc
    50401 attaatctcc tgcttacgat tttgctcgca gtcgttgccg gtacggcggg atgcatgctg
    50461 cgtcttcctt ccggcacgat gctcatcccg atgctggcgg gcgcagtgct ccagtctggt
    50521 cagctcatca ccatcgaact accggaatgg ctgctggcga tggcgtatat ggcaattggc
    50581 tggcggattg gtcttggttt cgataaacaa atcttactgc gggcactacg cccgctaccg
    50641 caaatcctgc tgtcgatttt tgctctgctg gctatttgtg caggtatggc gtggggactg
    50701 acccggttta tgcatattga ttttatgact gcctacctcg ccaccagtcc cggcgggctt
    50761 gataccgtgg cggtcatcgc cgcagggagc aatgccgata tggcgcttat tatggcgatg
    50821 caaaccctgc gcctgttcag tattttgctg acggggccag ccattgcgcg gtttatttca
    50881 acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg gacaccagta aaacgggcgc
    50941 gaggacaacg tggttttctc aatgatgccg ccacagcgtt cgcacagttc gccatcacga
    51001 tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat tctcatccac ctgcccccgc
    51061 gtagcgtaag aaaaccgagg aatctccagt aacgcgtgtg cgagtgcatc cagttgcgcc
    51121 gcattgagat ctttcgcttt atgatttcca gtcaacccaa cctgccagag gatctccacc
    51181 cgcaaataat tgccaagccc agccagaaac gcctgatcga gcagtaatcc tgcaaactga
    51241 cggttacgaa agcgcggcga caataaccgt tcctttacca cctccggcgt cagattcgga
    51301 tccagcacat cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg ttccggcctc
    51361 aacatctcaa tatccgaggc gctataaagc agaatggttt tgtcagccgt ttgcagtttt
    51421 acccgcaata ctcgcgtggt ctgcggctct tcgccggtat caaccacgcg ccagacgccg
    51481 taaagctgat tatggctgta gagcgttaaa ccgttgggaa aatgagttaa caacgcctta
    51541 ccacgcgttt ccacatgggt aacgtgttga ccgataagtt gtgattgata agtttttaac
    51601 tgcgggaagg caaaccagac atcagttagt ggtttgcctt tgatcgccgc ctccaggtta
    51661 tccgctgcac ggcggatctc cgggccttca ggcatggtga tatccttttt tcattcttta
    51721 ttaagcgggg ttatgctgcg acaacaatcc cctttttggc aaatgtagct cgtaatcggc
    51781 gggcgaacgc cagagcgtgc tcgccgtcgc catgcaggca gaccgtttgc gccgcgaccg
    51841 ttgcccattc gccggtgata cttttgactc tgccgtgttg caccatctcc agcgtttgcg
    51901 ccaacgcctg ttcttcgttt tcaatcaacg cgcctggctg acttcgcggc accagcgagc
    51961 cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg ccatattgct
    52021 tgcctgcacg aatcagctcg cttcctgcca gcccaacgag aaccaatgcc ggatcgcaag
    52081 cgtataccgc tctggcgatg gcgtctgcca gttgtgcttc tttcgccgcc tggttgtaca
    52141 acatgccgtg cggtttgaca tgacgcatta cgccgccttg cgcacgggca atcgttgcca
    52201 gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc cggcggcagc tgcatggcgc
    52261 tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc gccaatcgcg acaccatttt
    52321 ttattgcttc acgcacgcaa gcctgcatgg tttgcgcatc gcctgcatga aatccacaag
    52381 caatattggc agaggaaacc agcgttaata gctctgcgtc gctggcgcag ccttcgccca
    52441 gatcggcgtt caggtcaatt ttcattgtgc agccgccacg ctaattgttc aaaataacgt
    52501 tgctgatctt gccgcgcttt cagtgcctct tccagtgaac actggacaaa atgaatcggc
    52561 tgaccgagcg gaatttgcgc cagatggtac atatcagcct caatgataca ggcaatacgt
    52621 ggataaccac cggtggtctg tgcgtcgttc atcaacacaa tcggctgccc gttatgcggc
    52681 acctgcacca cgcccggtaa caaaccgtga gataacagtt cgcgatcggt gatgcgtttt
    52741 aaaatttgcc cctgtaaacg atagcccatt cggttacttt gcgagctaag ctgccagggc
    52801 gaacgccaga atgcatcctg cgaggcgcga tcgaactcat gatattccgg ccccggcaag
    52861 gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct gcgcttccat aaaatcacgc
    52921 ttcgatttgc caatcgggag tcagtcacca tccttcagta aacggccttc cagcccgcca
    52981 atccccactt tgagatcggt gctgcatgac cccattaccg gcggaacatc aataccaccc
    53041 gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt taagcgttaa acgctggcct
    53101 gctttcatcg gcaatcgcca gccggtccag acggcgttat catccaaccg cgcttcgcaa
    53161 cctgcacccg tcagggcaaa ccacccatca gtttcgaact caactgttaa ctgaccgagc
    53221 gtgatctcca gcgcgggggc attggcgtca ttacccacca gcaggttagc aatgcgcagc
    53281 gcaggcatat ccagtgcgcc acagtggctg atacccgact ggcaaaaacc gtgacgaccg
    53341 ccatcctgca cagtggtgta catgcccgca cgaataatct tcagcatatt ccctccttct
    53401 gcggtacaaa tcgcacgctg tctcccggac gtaataagat gggttcgtca cgcgccggat
    53461 caaacaggct gagtgaggta tgaccaatca actgccagcc acccggcgtt gccagcggat
    53521 aaacaccagt ctgcggcccg ccgatcccga cagaacctgc cggaacgagc aagcgcggtt
    53581 cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc gagatacggg aagcccggtt
    53641 gaaaacctaa aaaccagacc acgtattcca cggaggagtg caattcaaca acctgttttt
    53701 cgctcaaccc gcaatacgcc gcgaccaccg ccaaatccgg tccgcctgca ccaccgtaaa
    53761 ccaccggaat ttcaataaag cgagactcag gttccagggc ctcgctctcc tcccaccagc
    53821 gttgcaaacg ctctatggca tccagcgcca gcgactcagg attacgcaaa atcaccgtga
    53881 tattgttcat gccgggaatg gcttcaacca cattcggcat atccaccaga cgctgcgcca
    53941 gtcgccagat ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc agcactaccg
    54001 ccgtttcacc tatcagataa caacgcgctc gttgcacttt cgatccctct gattattcgt
    54061 tatgcaggat taggaatatc aataaaggtc acatcaagat cggtattttc attcagccac
    54121 tcgctcaatg cgcgaatacc accgcgttca gtggcatggt gacctgcagc ataaaaatgc
    54181 aatccctgct cgcgggctga atgaatggtc tgttcagaaa cttcgccagt aataaaagca
    54241 tccacgccaa aacgcgcggc gctatcgata aaactttgcc cgccgcccgt gcaccaggcg
    54301 acgcgctgga ccacctcagg tccggtatcg ccacaccata acggcttacg tcccagacgc
    54361 gcttcaatcc aggaggctag ttccattccc ggcacaggca tggtcagctc tccccacggc
    54421 accaacggct caatttcgcc catgaccgtg atccccagta acgccgccag ctgtgcgtta
    54481 ttgcccagct caggatgtgc gtcaagcggc aaatgccagc catacaggtt gatatcattc
    54541 gccagcaacg tttttaaacg gttacgcttc attccgcgaa tgaccggaga ctctcctttc
    54601 cagaagtagc catgatgcac aatgaccgca tccgcgccca gacgcactgc ctcatcgagc
    54661 aaagcctggc tggcggtgac accggtaaca attttttgca ccgtctcttt gccttccacc
    54721 tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt tcagtttttc gttaatcagt
    54781 tgttccagtt cggtgttttt catcttcgcg ccttccgtat tctgatagct acaccgttaa
    54841 catagcgtta catcgcctgt tcgtcgatag ccaatctttt gacatattaa gaaacaaacc
    54901 cgacgccaaa agacgcaaat tgtttatatc atcacgcttc agctaatgta tatttatgca
    54961 atcagacagc atattgcata aataatcaga tctgtatcaa agttttaact ttaaggaaac
    55021 acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa ttgtggaatt attatgaata
    55081 aacacgcatc acaaccgcgc gctatttact atgtcgttgc gctgcaaatc tgggaatatt
    55141 ttagctttta cggcatgcgt gccctgctga ttctctatct caccaatcaa ctaaaataca
    55201 acgatactca cgcctacgag ttatttagcg cctactgttc gctggtgtat gtcacgccaa
    55261 tcctcggtgg ctttttggcg gataaagttc tcggcaatcg catggcggtg atgctggggg
    55321 cgttgttgat ggcgatcggt catgtggtgc tgggtgccag tgagatccat ccgtcattcc
    55381 tctatctgtc cctggcgatt atcgtctgcg gctatggcct gtttaaatct aacgtaagct
    55441 gtctgctcgg cgagctgtat gagccaaccg atccgcgtcg tgatggcggt ttctcgctga
    55501 tgtatgcggc gggtaacgtg gggtctatta tcgcacctat cgcctgtggt tacgcccagg
    55561 aagagtacag ctgggcgatg ggctttggcc tggcggcggt tggaatgatc gcgggtctgg
    55621 tcattttctt atgtggcaat cgtcatttca ctcatacccg cggcgttaac aaaaaagtac
    55681 tgcgtgcgac aaactttctc ctgccgaact ggggatggct gctggttctg ctggtggcaa
    55741 cgcctgcact gattaccgtg ctgttctgga aagagtggtc ggtatacgcc ttaattgtcg
    55801 cgactattat tggcctgggt gtactggcaa aaatttatcg caaagcagaa aaccagaaac
    55861 agcggaagga gctggggctg attgtgacgc tcaccttctt cagtatgttg ttctgggcct
    55921 tcgcacaaca gggcggtagc tcgattagcc tttatatcga ccgcttcgtt aaccgcgata
    55981 tgtttggtta taccgttccg accgcgatgt tccagtcgat taatgccttc gcagttatgc
    56041 tgtgcggtgt gttcctggcg tgggtggtta aagaaagcgt cgcaggtaat cgtaccgtgc
    56101 gcatctgggg aaaatttgct cttggccttg gcctgatgag cgccggattc tgcattctga
    56161 ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc tctgccactg atggtgttag
    56221 gcctcgcggt gatgggcttt gcggaactgt ttatcgaccc ggttgccatg tcgcaaatta
    56281 cgcgcattga aattccgggc gtgaccggcg tattaaccgg catctacatg ctgctttctg
    56341 gcgcgatcgc gaactatctg gcgggcgtga ttgccgatca gacatcgcag gcttcgtttg
    56401 atgcttccgg ggcgatcaac tactccatca atgcatatat tgaagtattt gatcaaatta
    56461 cctggggcgc actggcgtgt gtaggactgg tactgatgat ttggctgtat caggcgctga
    56521 aattcagaaa ccgcgcgctg gcgctggagt cataataggc cagcaccgat gcctgatgcg
    56581 acgcttgacg cgtcttatca ggcctacgta tttcctgcaa tttattgaat ttgcacaaat
    56641 ttgtaggctg gattaggcgt tcacgccgca tccggcatct gggctcgatt gcctgacgcg
    56701 tctgttattt ccccttccgc gccgcctcat acgctgccaa cgtttgtact ctcgcctctt
    56761 tgtgctcgac tatcggttgc ggataatcca gcttcacacc tgctttctgc gcccacttcc
    56821 acggctcatg caccgctttc cctggtacat cgcgcagctc cggtaaccat cgacgaataa
    56881 actcgccctc acggtcaaat ttctcgccct gagttatcgg attgaaaata cgaaaatacg
    56941 gcgcagcatc agtacccgta gaagccgccc actgccagcc accgttatta gctgccaaat
    57001 caccatcaat cagctgcgac atgaaatatc gctcgccttc gcgccagtcg atcaacaaat
    57061 ctttcaccag aaaactggct gtaatcatcc gtagccggtt atgcatccag ccagtgctgt
    57121 taagctgacg catagcggca tcaataatcg ggtatcccgt tttgcctttc tgccaggcct
    57181 gtaaatgtgc gggattgctt tgccactgca cacgatccgt ccaggcaata aatggacgat
    57241 gtttacacaa cgaggggtaa tacgtcatca gatgacggta gaactcgcgc cagatcagct
    57301 cactaagcca gacactaccg gccccaccgt ccagcgcctg cggctgttcc gccaacaagc
    57361 gatgcaaaca ctggcgaggc gataacccgc ccgttgccaa gctggcggac aaacggctgg
    57421 tgccttccac tgccggaaaa tctcgttgtt gctcatactc tccagcaccg ttctcgcaaa
    57481 actggcgtaa ttgcgcaatc gccgcttttt cttccaccgg gaaatgcgca gtatcgaaag
    57541 actgacgagg ataattcagc gtaatggatg gcgcgggctt tatcgatccg ctactacgaa
    57601 cttttggtgc agcgacgcac tccggcatcc cttcccgcag ccgtttcagc caggcattct
    57661 taaaaggcgt aaagactttg tacatctcgt gattgccggt catcaccgcc cccggcggca
    57721 ggatcacgct gtcatcaaat ccttcacaca ccacgttacg cagcgctctt tcaacgtcca
    57781 catcccgcgc tcgctcattc acctcatact gatagttata aaaaagatga gtaacgctgt
    57841 tttccgcgca cacctgttta actatttcga cactggcgac aaagtcatcc acttcacgga
    57901 acaataaagg aatacctttt tccgccagcg cgatttgtag cccattcagt tgagcattga
    57961 taagttcagc ctgacgcggc gacatgttat gcgtctccca cttgcgtggt gtagcgatat
    58021 acagcgccag cacgcgtgca gacgaattgc ggcaggcggc agccagtgcg agattatcgt
    58081 gcagacgtaa atcctggcga aaccaggcca gatgggtagt cataaaactc ctgaatggcg
    58141 caagttaatc acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg
    58201 ataaagcgcc atatagtgtt tgatacgcat cagcggcgca agaagtggtt gctcgccacg
    58261 gcgataactg tcgataagcg cagccagctg ctggcgttct gtggaatcaa tatgcgggcg
    58321 aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg cgggatgaga
    58381 aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa tcatccagat tttgccactg
    58441 atgaatatca gccacaaacg ggccaagttt gcggtactcg ggctgagaat gggccagaaa
    58501 gacgagttta tagcggctgt gataatcgag aagcgccccg cgcgttaagc catgttcttt
    58561 cagtcgatgt aactcatgaa gggcgaagat acggattagg gtgctgtcat caaatcgttg
    58621 tagattcatt actgctcctg aacggtatac aggagtaagt gtaggcgttg gtaaatgatg
    58681 tttgtgaagt taatccgcta gcagggcaag attacgaaag cgcgctcccc gcaagcaact
    58741 aatcaaggta gttaatcatg gctgaacacg atattacgcc agaagtcact atttccaaaa
    58801 cacagcgccg ctataaagtc ggctatgtca gagcccgcca ccaggaccgc aaaaccggta
    58861 tagcccggta ttattcacaa catcccagtc tgcaccttaa gggcgactgg ctgaaagagg
    58921 ccggatttga taccggacgg ggtgtcacgg tgaagatttc ggaggggggc ctgaccatca
    58981 tcgctgacag tgacgaggtg caggaactgc gggatgggat gtttagtgtg ctgaacgaaa
    59041 gttaaatatt aaaaaagctg ggaagattta aaaccttcct agctttttat tctgtcttat
    59101 tataatggat actcatcatc ttccgatgaa tttataaatt gctccaagtt tacatgaaga
    59161 gtacttaacc tagtttttaa ctcattaagt gaagcgataa tttcatattt tccaacataa
    59221 tcatatgatg gatcgtattc attatctatg tcagaagcta aaaatgatga tattcgtagt
    59281 ttaaatttag cttcatcttt attacattca ttggcataag gtgaatgccc tatcatagca
    59341 tattcatcta atctaaaagg ataagatggt tttaattcat cgatcaatga aatttcaatc
    59401 catgttctat atccaggcat taatggacta atttctttga gcaaaacctt tccacttaat
    59461 aatgtattga gattgttcat tattatgtct cccacgggaa aaatatatgt ctaacacttc
    59521 caccatgttg atgaacttgt aaatgtggta aatcaccatc aggatttcct ggcccatgac
    59581 ctgacactct atatgaaggt tttgttacag aaggatcttt tatatcatcc caatgatatg
    59641 aacctgcttt tgttttattt tcagtaagat attccattaa gttcttagtg ctggggcctg
    59701 actcacctgt catattttta taattatgac ctaaatatct atccataaac aattgttcag
    59761 cgtcacttct agacctaaca gtaactttgg tttcactact atctaataaa cgattcgctt
    59821 tttggttact aacagcacat ggagttaatc caagtggatc agcatatttt agaggattag
    59881 gtgcataact gtagagattt aaaccgcctc tcaaccctat cgggtcagtt accatgaacc
    59941 gcccgacatc cggatcatag tacctgtgca gattgtagtg cagccccgtt tccctgtcga
    60001 ggtactgtcc ctggtagcgc aggttctgct ccaccccgcc gtggtccttc tcctgtatcg
    60061 tgttacccca gacctgatac cccgtctccc acacgatttt cccgtcgctg tccgtcatct
    60121 cttccggcgc gccgttcaca tccgtgtgaa aatacagtat ccggttcggg ccctctttgc
    60181 ccgccttatc cacccgcgcc agcggttcat aactcccctg gtccctgtag atgtacaggc
    60241 tgcctgttcc ctccggcctg ctctcctgcg ccagccgcat tccgtcccat aaaaattttg
    60301 tccggttgta cggcttaccg tcagggctga tgtgctgttt ttccgtccgc ctccccagtg
    60361 catcgtacac ataccggtat gtcccccgcg ctgtcgtcac ttccaccatc cggtgttccg
    60421 catcataacg gtaactctgc gttccgctcc ggccctcctt ttccaccgca cggccaaatt
    60481 cgtcataacg gtagtgcaca ccacggtaag acatcagccg gttgaacggc accacgctgt
    60541 tttctgccgt ggccctttcc cccgcgcgcc tccccagcag gtttcccgcc gcgtccacct
    60601 ccgtttccgg ctggtgccac accgggtgcc actgcgcctg ctccacccgc cccagcgggt
    60661 cgtgcgtctc cgtgatatgc ccctgcaggt catacacata gcgccatttg ccgccctgag
    60721 ggtcggtcat ccccagcaac agtcgttctt cgtcgctcca gcggaatgtc gtcacctgtc
    60781 cggcttcgtc ctggtacgcc gtcacctgat tctgcgcatc ccactggtga taacgtgtgc
    60841 gccccagtcc gtccgtcacg cacagcctgc gctgcgccag gtcccacgcc agccggtact
    60901 gttcaccgct gtccgtgtgg tgccccgtca cccgccatgc cgcgtcatcc ggaccttcat
    60961 accagcccca ctggtaataa tacccttcgc cgcccggctg gcggtgcagc gtcatgcagt
    61021 ggttttcgtc gtaagcaaac tcacgcagca ggtgccccgt gctgtcgtac acgccgttca
    61081 ggtggccggc ctcgtcatag ctgtaacgac gcagcagtaa aggtgcagtc tcagggaaat
    61141 gctgataaat ctgtgtcacc cgccggggat gcgccgccag ttcgtaatac agacggatgc
    61201 acggacgctg ctgttcgccg ctgatttcca caatgcggcc ccggtcgtcg tagtacagat
    61261 tcagacagtt gctgttacgg tcgcccagca ttttcagccg ctggcgctgc gggtggtgcg
    61321 ggtcagcctc aaacagaaaa cactgcccgt catcgctgct gataagccag tgcccctgct
    61381 caccacgcct taccgccagc ccttcaccgg ggctgtaaaa cccgcccccc ggcagtacgg
    61441 cttccatatc caagcgtcgc cccatgcccg acacaaaggt catgcagttt tcgtctgcgc
    61501 tacccggtgt gcacacgagg ctgacttcat acattacact ccagccctga ccaaacattc
    61561 cttccgtacg ttcgtcacga ctgctgtaca cgcgctgcca gtccagcgga aagtggcccg
    61621 gcagactgaa atcgaagtca tcgtcatcgg ccaggtattt ggccccggtg gcggcattga
    61681 ccggatgcgg aagggcactg gtggccttct gcgtcagcat gctcatcccg acactggcag
    61741 caaaacaggc gattttagaa agcattttgc ccgggcgaag aaacatcagc gccacggttg
    61801 ccaccagggt tatctgcgac ctgccgcttc tgatatccct gaccaccagt aacggaccgc
    61861 caatgcgcac atccggtgac acaaacgccc cgttccccgg ctcattcacc accctggcct
    61921 cgcaggtaca gcgtgccccg ctgcgtgtcg ccggctggct gttgatacgc acatcccgcg
    61981 cgccttccgc gatatattcc gctgcgggcg gctcgtcatc gcctttgaac agattactga
    62041 tgctgccaat aaccgcattc actccctgaa aggcggtttc cagcataccg cccggattca
    62101 gcaggttaag ggccgttccc agggggtcat ccagaaaggc ggcttttttt tgtgcgagaa
    62161 actgcgtcag tgaggaagga tgtttatggc agtcgatttt atcgtcaggg gtcggggtgg
    62221 atcccggggc cgcactggcc acagtgggct gccatagttc cgacacaaaa tgtgtcacgc
    62281 catccaccat ttcagaacgc ccttcttccg tactgagctg gtcgtgcaga gcccgggaca
    62341 tcagcacagg ccctgtcacc gggttaaccg ccagggctat ggcccccgcc atttccagat
    62401 attccccggc cacatcccgg aacgttctgg catcctcctg acgctgcgct tcttcctgtt
    62461 ctgccttcgc ctgcgcttcc tgcgcctcta tgtcctgccg cgatgccgca gtcgccgctg
    62521 cccgcgcggc aggtttgctg ttgatccatg tgtcaccaga gccggtgagg atgtaacctt
    62581 caatctgagg cgggaaaagc atatttgcga gggctgaaca ggcttcgctg accttttctg
    62641 tcacgccggt gtacaccatc gccacacccg ccacaaaacc ggtcagcacg acggccgtgc
    62701 accccatcgt gatccccact gacgtcgcca gtaacgaggt tgtgccgaca atgagggcat
    62761 caataacgac atttgcggcc acctccagca ccgcccccag cacatctgcc ataaacgacg
    62821 tatgcagcag ggcatcaccc tgtcttgccg cccagaatgc ctcttccatg tcccgttccc
    62881 tgtatgatta attcgtcctg acttcctgcc gcatcctgaa gcgcagattc tttttaattt
    62941 cagcccagcg cgtcatatct gccggggtca gcggtgtggt tctggcgtag gtgaaggcca
    63001 tcatgacccg ctcacccggc aatacaaatg ccacctgaca ctggtacacc tgctcgttac
    63061 cccggagaaa ctggctctgt atttccaaac cgcctcgttg agataatatt ccgtcataat
    63121 gtaaatagtc cgttttaata aaggaattat ttattgacag gttctgtttt tttaatcatg
    63181 aatgtaatac ccatcaccgc tttacagcac actttatctt tccagtcata atcactaata
    63241 atattaagca attagcaata tttacaacga ccagagcgga tacatagtta tgtatttaac
    63301 aatcccaata tattcactat gcaaataaaa ccactagtaa cagacacgct accgcctcca
    63361 tataaaaatg gacaccttat aaaattgtat cttgcataga aaaagctgaa atatcggatg
    63421 cgttttaatt atcaactata aatctataag ctttttatta acaac
//
LOCUS       DAJTRH010000012        60414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTRH010000012 DAJTRH010000000
VERSION     DAJTRH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..316
                     /locus_tag="OO339_000835"
     CDS             <1..316
                     /locus_tag="OO339_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5925565.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="OO339_000836"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="OO339_000836"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5925566.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="OO339_000837"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="OO339_000837"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5925567.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO339_000838"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO339_000838"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5925568.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="OO339_000839"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="OO339_000839"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5925569.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="OO339_000840"
     CDS             complement(3720..4949)
                     /locus_tag="OO339_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5925570.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="OO339_000841"
     CDS             complement(5013..5453)
                     /locus_tag="OO339_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5925571.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="OO339_000842"
     CDS             complement(5558..6328)
                     /locus_tag="OO339_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5925572.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="OO339_000843"
     CDS             complement(6325..7251)
                     /locus_tag="OO339_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5925573.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="OO339_000844"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="OO339_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5925574.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO339_000845"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO339_000845"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5925575.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="OO339_000846"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="OO339_000846"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5925576.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO339_000847"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO339_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5925577.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="OO339_000848"
     CDS             12958..13305
                     /locus_tag="OO339_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5925578.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="OO339_000849"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="OO339_000849"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5925579.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="OO339_000850"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="OO339_000850"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5925580.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="OO339_000851"
     CDS             complement(15125..15487)
                     /locus_tag="OO339_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5925581.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO339_000852"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO339_000852"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5925582.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="OO339_000853"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="OO339_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO339_000854"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO339_000854"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5925583.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO339_000855"
     CDS             complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO339_000855"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5925584.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO339_000856"
     CDS             complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO339_000856"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5925585.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO339_000857"
     CDS             complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO339_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5925586.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28311..29684
                     /gene="aroP"
                     /locus_tag="OO339_000858"
     CDS             28311..29684
                     /gene="aroP"
                     /locus_tag="OO339_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5925587.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OO339_000859"
     CDS             complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OO339_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5925588.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OO339_000860"
     CDS             complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OO339_000860"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5925589.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31217..32110
                     /gene="nadC"
                     /locus_tag="OO339_000861"
     CDS             31217..32110
                     /gene="nadC"
                     /locus_tag="OO339_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5925590.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32313..32753
                     /gene="ppdD"
                     /locus_tag="OO339_000862"
     CDS             32313..32753
                     /gene="ppdD"
                     /locus_tag="OO339_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5925591.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32763..33910
                     /gene="tadA"
                     /locus_tag="OO339_000863"
                     /pseudo
     CDS             32763..33910
                     /gene="tadA"
                     /locus_tag="OO339_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33900..35102
                     /gene="hofC"
                     /locus_tag="OO339_000864"
     CDS             33900..35102
                     /gene="hofC"
                     /locus_tag="OO339_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5925592.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO339_000865"
     CDS             complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO339_000865"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5925593.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36342..36380)
                     /locus_tag="OO339_000866"
     CDS             complement(36342..36380)
                     /locus_tag="OO339_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925594.1"
                     /translation="MIIKTLKMSARK"
     gene            36405..37025
                     /gene="coaE"
                     /locus_tag="OO339_000867"
     CDS             36405..37025
                     /gene="coaE"
                     /locus_tag="OO339_000867"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5925595.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37025..37768
                     /gene="zapD"
                     /locus_tag="OO339_000868"
     CDS             37025..37768
                     /gene="zapD"
                     /locus_tag="OO339_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5925596.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37778..37975
                     /gene="yacG"
                     /locus_tag="OO339_000869"
     CDS             37778..37975
                     /gene="yacG"
                     /locus_tag="OO339_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5925597.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO339_000870"
     CDS             complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO339_000870"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5925598.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO339_000871"
     CDS             complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO339_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5925599.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO339_000872"
     CDS             complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO339_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5925600.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO339_000873"
     CDS             complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO339_000873"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5925601.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO339_000874"
     CDS             complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO339_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5925602.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO339_000875"
     CDS             complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO339_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5925603.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO339_000876"
     CDS             complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO339_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5925604.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO339_000877"
     CDS             complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO339_000877"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5925605.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO339_000878"
     CDS             complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO339_000878"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5925606.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO339_000879"
     CDS             complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO339_000879"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5925607.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO339_000880"
     CDS             complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO339_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5925608.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OO339_000881"
     CDS             complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OO339_000881"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5925609.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OO339_000882"
     CDS             complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OO339_000882"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5925610.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OO339_000883"
     CDS             complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OO339_000883"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5925611.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OO339_000884"
     CDS             complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OO339_000884"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5925612.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OO339_000885"
     CDS             complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OO339_000885"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5925613.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OO339_000886"
     CDS             complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OO339_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5925614.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OO339_000887"
     CDS             complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OO339_000887"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5925615.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OO339_000888"
     CDS             complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OO339_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5925616.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14470 a        16732 c        14847 g        14365 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgtccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcaca agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcaccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc
    23341 ataatcagcg aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg
    23401 cctgcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac
    23461 tgttcagcgg caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca
    23521 ccgccgatat ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc
    23581 ggagtcgctt cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag
    23641 accataatca gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg
    23701 gtacctgcaa acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc
    23761 gactgttcag cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg
    23821 tcgctgccga tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc
    23881 ggagctgctt ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg
    23941 gaatcgaaaa tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc
    24001 tctttaacga tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg
    24061 gtgatcagcg actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt
    24121 tcaacttcat cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct
    24181 cttacgccag acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag
    24241 caaccacttt cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga
    24301 cataagaagc atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga
    24361 agccatcagt acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag
    24421 cgaacagttt catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga
    24481 tatacggaac gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac
    24541 catcacgcgc cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat
    24601 ctttcgccag gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca
    24661 gctgaacttt acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac
    24721 cttcctcagc accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt
    24781 agtagtaaac gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga
    24841 cagcaacttc gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact
    24901 gaatgtggct gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg
    24961 aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc
    25021 cgatacgctg gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt
    25081 tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc
    25141 cttcctgcag aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct
    25201 gcggggtgta ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca
    25261 taccgaaagt acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt
    25321 tcttcagcat cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct
    25381 gctcttccag cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt
    25441 tcggctgacg gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat
    25501 gctcttcaga accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca
    25561 ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct
    25621 ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag
    25681 caaggattac tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt
    25741 tcttcggatc gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa
    25801 ccagtgctgc ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt
    25861 tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac
    25921 cgctggtatc tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt
    25981 tccagccagc accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga
    26041 ccgggccgtc aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt
    26101 cacgggtagc gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga
    26161 ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca
    26221 ggaatttagc ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact
    26281 gccagaattc cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt
    26341 cctgacggaa gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt
    26401 aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc
    26461 gtgcacggaa gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg
    26521 ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt
    26581 tccagcggat agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt
    26641 gttcttcaac ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta
    26701 caccgccttt gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac
    26761 cttcttcacg gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca
    26821 cgtcatttgg gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt
    26881 tctggaacct gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta
    26941 gttaccgcgc ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa
    27001 aatcactaat tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca
    27061 cggcttctgt cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc
    27121 gcttcttccg gcttaccgac cataatcgct tcaaatatgc gggtgcggtg actactcacc
    27181 agcggcagca tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc
    27241 aacatcggct ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg
    27301 acggcaatct gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc
    27361 gccagctcta tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc
    27421 agcgcggcgt aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac
    27481 tgtgattcag gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg
    27541 ctgctctgga caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga
    27601 cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt
    27661 tccggtggga gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc
    27721 tgctcaatca catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt
    27781 cttaagccac ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta
    27841 acaaagtatt caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt
    27901 ttaacaactt gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac
    27961 gtaggaaagg gattttttaa cgattattaa actataaaaa tccgaattga accgattcac
    28021 ttaccaattt tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga
    28081 gaggggttga ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg
    28141 cataaagcgg gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt
    28201 gtaacaattc aaacctcctt gatgtaaaca aatcaataca acaaacggaa ttgcaaactt
    28261 acacacgcat cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag
    28321 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
    28381 cgctgggtgg cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg
    28441 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
    28501 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
    28561 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
    28621 ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg
    28681 aaatcccaac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
    28741 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg
    28801 tagtagcgat gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg
    28861 cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg
    28921 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
    28981 cagcagaagc tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc
    29041 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
    29101 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
    29161 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
    29221 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc
    29281 tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta
    29341 cagcattgtg cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg
    29401 cactggtggt atccgcactg gtgatcaact gggcgatgat cagtctggcg catatgaagt
    29461 tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc
    29521 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
    29581 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
    29641 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
    29701 cccgtacagg gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca
    29761 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
    29821 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc
    29881 tgatattgcg aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca
    29941 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
    30001 tgaagcactg catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc
    30061 cagtattgcc atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc
    30121 cccacaatca gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
    30181 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
    30241 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
    30301 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
    30361 cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
    30421 gtcacaccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
    30481 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
    30541 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
    30601 gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
    30661 tatcacaatg gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg
    30721 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa
    30781 gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg
    30841 catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
    30901 aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat
    30961 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
    31021 taatattgtg caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg
    31081 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
    31141 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc
    31201 tatctggagt tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct
    31261 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
    31321 aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg
    31381 ctctcatgcc acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga
    31441 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
    31501 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
    31561 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
    31621 acgccactat gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac
    31681 cttacctggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca
    31741 ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg tctccggctc
    31801 agtgcgccag gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga
    31861 agtagagaat ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct
    31921 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
    31981 actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt
    32041 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
    32101 ttttcgctaa ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
    32161 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
    32221 tcggaatctg ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa
    32281 agtagcacca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
    32341 tcgaactgat ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt
    32401 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
    32461 gtaccgccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
    32521 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
    32581 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag
    32641 gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca
    32701 gcgcattgca gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
    32761 caatgaatat tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg
    32821 ccagcgaaga agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg
    32881 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
    32941 aaggtcacgc cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc
    33001 ccaaagcaga gttgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
    33061 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
    33121 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc
    33181 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
    33241 tggcaggaaa caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
    33301 tggtattaag gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc
    33361 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
    33421 taactggccc tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa
    33481 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc
    33541 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    33601 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    33661 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    33721 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    33781 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    33841 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    33901 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    33961 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    34021 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    34081 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    34141 tggctctgct ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg
    34201 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    34261 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    34321 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    34381 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    34441 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    34501 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    34561 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    34621 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    34681 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    34741 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    34801 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    34861 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    34921 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    34981 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    35041 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    35101 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    35161 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    35221 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    35281 ctggcctcgc agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc
    35341 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    35401 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    35461 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    35521 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    35581 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    35641 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    35701 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    35761 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    35821 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    35881 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    35941 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    36001 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    36061 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    36121 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    36181 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    36241 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    36301 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    36361 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    36421 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    36481 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    36541 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    36601 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    36661 acctgctgca tccgctgatt caacaagaga cgcaacacca gatccagcaa gcaacttccc
    36721 cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc
    36781 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    36841 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    36901 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    36961 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    37021 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    37081 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    37141 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    37201 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    37261 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt
    37321 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    37381 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    37441 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    37501 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    37561 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    37621 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    37681 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    37741 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    37801 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    37861 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    37921 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    37981 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    38041 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    38101 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    38161 cgggttgccc ttctttaccc cacggccccc cttcccaact ttcgaccagc cagaaccaca
    38221 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    38281 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    38341 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    38401 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    38461 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    38521 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    38581 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    38641 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    38701 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    38761 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    38821 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    38881 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    38941 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    39001 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    39061 ccactcggta attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    39121 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    39181 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    39241 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    39301 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    39361 cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga
    39421 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    39481 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    39541 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    39601 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    39661 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    39721 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    39781 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    39841 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    39901 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    39961 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    40021 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    40081 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    40141 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    40201 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    40261 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    40321 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    40381 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    40441 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    40501 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    40561 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    40621 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    40681 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    40741 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    40801 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    40861 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    40921 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    40981 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    41041 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    41101 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    41161 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    41221 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    41281 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    41341 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    41401 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    41461 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    41521 aagaaagatg acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt
    41581 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    41641 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    41701 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    41761 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    41821 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    41881 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    41941 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    42001 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    42061 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    42121 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    42181 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    42241 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    42301 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    42361 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    42421 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    42481 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    42541 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    42601 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    42661 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    42721 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    42781 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    42841 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    42901 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    42961 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    43021 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    43081 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    43141 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    43201 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    43261 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    43321 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    43381 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    43441 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    43501 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    43561 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    43621 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    43681 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    43741 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    43801 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    43861 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    43921 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    43981 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    44041 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    44101 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    44161 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    44221 tgcctgtcgc ctgagtccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    44281 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    44341 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    44401 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    44461 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    44521 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    44581 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    44641 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    44701 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    44761 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    44821 cagcataagg aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca
    44881 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    44941 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    45001 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    45061 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    45121 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    45181 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    45241 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    45301 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    45361 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    45421 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    45481 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    45541 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    45601 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    45661 ctgtaaaacc ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc
    45721 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    45781 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    45841 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    45901 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    45961 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    46021 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    46081 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    46141 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    46201 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    46261 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    46321 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    46381 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    46441 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    46501 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    46561 cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat
    46621 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    46681 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    46741 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    46801 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    46861 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    46921 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    46981 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    47041 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    47101 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    47161 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    47221 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    47281 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    47341 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    47401 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    47461 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    47521 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    47581 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    47641 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    47701 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    47761 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    47821 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    47881 gcattcaggg tgacgcacat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    47941 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    48001 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    48061 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    48121 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    48181 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    48241 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    48301 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    48361 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    48421 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    48481 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    48541 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    48601 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    48661 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    48721 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    48781 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    48841 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    48901 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt
    48961 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    49021 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    49081 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    49141 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    49201 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    49261 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    49321 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    49381 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    49441 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    49501 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    49561 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt
    49621 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    49681 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    49741 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    49801 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    49861 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    49921 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    49981 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    50041 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    50101 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    50161 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    50221 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    50281 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    50341 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    50401 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    50461 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    50521 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    50581 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    50641 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    50701 tgccccttta acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag
    50761 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    50821 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    50881 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    50941 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    51001 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca ggatactgaa
    51061 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    51121 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    51181 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    51241 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    51301 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg
    51361 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    51421 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    51481 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    51541 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccacc
    51601 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    51661 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    51721 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    51781 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    51841 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    51901 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    51961 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    52021 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    52081 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    52141 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    52201 ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg
    52261 tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata
    52321 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    52381 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    52441 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    52501 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    52561 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    52621 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    52681 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    52741 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    52801 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    52861 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    52921 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    52981 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    53041 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    53101 agaaatattt ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat
    53161 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    53221 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    53281 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    53341 cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga
    53401 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    53461 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    53521 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    53581 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    53641 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    53701 gcggtcaata ccagcagctt ttgccgcctc acccacctgt acatggcagg cttcgctttc
    53761 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    53821 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    53881 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    53941 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    54001 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    54061 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    54121 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    54181 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    54241 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    54301 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat
    54361 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    54421 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    54481 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    54541 cgtcagagca accacgagcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    54601 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    54661 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg
    54721 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    54781 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    54841 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    54901 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    54961 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    55021 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    55081 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    55141 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    55201 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    55261 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    55321 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    55381 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    55441 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    55501 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    55561 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    55621 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    55681 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    55741 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    55801 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    55861 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    55921 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    55981 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    56041 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    56101 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    56161 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    56221 acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat ctctcgcagt
    56281 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    56341 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    56401 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    56461 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    56521 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    56581 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    56641 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    56701 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    56761 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    56821 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    56881 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    56941 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgtta acgccagctt
    57001 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    57061 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    57121 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    57181 cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg tgccgctcag
    57241 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    57301 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcgttgt tcagttcgcg
    57361 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    57421 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    57481 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    57541 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    57601 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    57661 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    57721 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    57781 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    57841 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    57901 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    57961 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    58021 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    58081 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    58141 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    58201 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    58261 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    58321 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    58381 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    58441 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    58501 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    58561 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    58621 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    58681 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    58741 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    58801 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    58861 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    58921 gcaatgcgtt tggcaaaatg ctcttcacca taggttttca atacccaggc gatatcggct
    58981 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    59041 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    59101 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    59161 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    59221 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    59281 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    59341 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    59401 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    59461 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    59521 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    59581 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    59641 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcaacagac gcgataattt
    59701 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    59761 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg
    59821 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    59881 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    59941 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    60001 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    60061 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    60121 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    60181 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    60241 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    60301 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    60361 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt gatg
//
LOCUS       DAJTRH010000013        59861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTRH010000013 DAJTRH010000000
VERSION     DAJTRH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            523..1812
                     /gene="sdaC"
                     /locus_tag="OO339_000889"
     CDS             523..1812
                     /gene="sdaC"
                     /locus_tag="OO339_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5925617.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1870..3237
                     /gene="sdaB"
                     /locus_tag="OO339_000890"
     CDS             1870..3237
                     /gene="sdaB"
                     /locus_tag="OO339_000890"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5925618.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3348..4103
                     /gene="xni"
                     /locus_tag="OO339_000891"
     CDS             3348..4103
                     /gene="xni"
                     /locus_tag="OO339_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5925619.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4156..5304)
                     /gene="fucO"
                     /locus_tag="OO339_000892"
     CDS             complement(4156..5304)
                     /gene="fucO"
                     /locus_tag="OO339_000892"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5925620.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5332..5979)
                     /gene="fucA"
                     /locus_tag="OO339_000893"
     CDS             complement(5332..5979)
                     /gene="fucA"
                     /locus_tag="OO339_000893"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5925621.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6526..7842
                     /gene="fucP"
                     /locus_tag="OO339_000894"
     CDS             6526..7842
                     /gene="fucP"
                     /locus_tag="OO339_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5925622.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7875..9650
                     /gene="fucI"
                     /locus_tag="OO339_000895"
     CDS             7875..9650
                     /gene="fucI"
                     /locus_tag="OO339_000895"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5925623.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9759..11178
                     /gene="fucK"
                     /locus_tag="OO339_000896"
                     /pseudo
     CDS             9759..11178
                     /gene="fucK"
                     /locus_tag="OO339_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11180..11602
                     /gene="fucU"
                     /locus_tag="OO339_000897"
     CDS             11180..11602
                     /gene="fucU"
                     /locus_tag="OO339_000897"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5925624.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11660..12391
                     /gene="fucR"
                     /locus_tag="OO339_000898"
     CDS             11660..12391
                     /gene="fucR"
                     /locus_tag="OO339_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5925625.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12435..13535)
                     /gene="rlmM"
                     /locus_tag="OO339_000899"
     CDS             complement(12435..13535)
                     /gene="rlmM"
                     /locus_tag="OO339_000899"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5925626.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13528..13923)
                     /locus_tag="OO339_000900"
     CDS             complement(13528..13923)
                     /locus_tag="OO339_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5925627.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13942..14859)
                     /gene="gcvA"
                     /locus_tag="OO339_000901"
     CDS             complement(13942..14859)
                     /gene="gcvA"
                     /locus_tag="OO339_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5925628.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15210..15437)
                     /locus_tag="OO339_000902"
     CDS             complement(15210..15437)
                     /locus_tag="OO339_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5925629.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15629..16834
                     /gene="csdA"
                     /locus_tag="OO339_000903"
     CDS             15629..16834
                     /gene="csdA"
                     /locus_tag="OO339_000903"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5925630.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16834..17277
                     /gene="csdE"
                     /locus_tag="OO339_000904"
     CDS             16834..17277
                     /gene="csdE"
                     /locus_tag="OO339_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5925631.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17328..18134)
                     /gene="tcdA"
                     /locus_tag="OO339_000905"
     CDS             complement(17328..18134)
                     /gene="tcdA"
                     /locus_tag="OO339_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5925632.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18372..19469)
                     /gene="mltA"
                     /locus_tag="OO339_000906"
     CDS             complement(18372..19469)
                     /gene="mltA"
                     /locus_tag="OO339_000906"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5925633.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19678..19754
                     /locus_tag="OO339_000907"
     tRNA            19678..19754
                     /locus_tag="OO339_000907"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19712..19714,aa:Met,seq:cat)
     gene            19788..19864
                     /locus_tag="OO339_000908"
     tRNA            19788..19864
                     /locus_tag="OO339_000908"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19822..19824,aa:Met,seq:cat)
     gene            complement(19939..21192)
                     /gene="amiC"
                     /locus_tag="OO339_000909"
     CDS             complement(19939..21192)
                     /gene="amiC"
                     /locus_tag="OO339_000909"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5925634.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21424..22755
                     /gene="argA"
                     /locus_tag="OO339_000910"
     CDS             21424..22755
                     /gene="argA"
                     /locus_tag="OO339_000910"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5925635.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22817..24643)
                     /gene="recD"
                     /locus_tag="OO339_000911"
     CDS             complement(22817..24643)
                     /gene="recD"
                     /locus_tag="OO339_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5925636.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24643..28185)
                     /gene="recB"
                     /locus_tag="OO339_000912"
     CDS             complement(24643..28185)
                     /gene="recB"
                     /locus_tag="OO339_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5925637.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28178..31066)
                     /gene="ptrA"
                     /locus_tag="OO339_000913"
     CDS             complement(28178..31066)
                     /gene="ptrA"
                     /locus_tag="OO339_000913"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5925638.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31242..34610)
                     /gene="recC"
                     /locus_tag="OO339_000914"
     CDS             complement(31242..34610)
                     /gene="recC"
                     /locus_tag="OO339_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5925639.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34623..34982)
                     /locus_tag="OO339_000915"
     CDS             complement(34623..34982)
                     /locus_tag="OO339_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5925640.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVLMVMIVTALSG
                     IQRTSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVS
                     PGGREGEMTRLHCPNCQ"
     gene            complement(34967..35374)
                     /locus_tag="OO339_000916"
     CDS             complement(34967..35374)
                     /locus_tag="OO339_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5925641.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35371..35934)
                     /locus_tag="OO339_000917"
     CDS             complement(35371..35934)
                     /locus_tag="OO339_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5925642.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35925..36395)
                     /locus_tag="OO339_000918"
                     /pseudo
     CDS             complement(35925..36395)
                     /locus_tag="OO339_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36579..37373)
                     /gene="thyA"
                     /locus_tag="OO339_000919"
     CDS             complement(36579..37373)
                     /gene="thyA"
                     /locus_tag="OO339_000919"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5925643.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37380..38255)
                     /gene="lgt"
                     /locus_tag="OO339_000920"
     CDS             complement(37380..38255)
                     /gene="lgt"
                     /locus_tag="OO339_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5925644.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38406..40652)
                     /gene="ptsP"
                     /locus_tag="OO339_000921"
     CDS             complement(38406..40652)
                     /gene="ptsP"
                     /locus_tag="OO339_000921"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5925645.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40665..41195)
                     /gene="rppH"
                     /locus_tag="OO339_000922"
     CDS             complement(40665..41195)
                     /gene="rppH"
                     /locus_tag="OO339_000922"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5925646.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41880..42569
                     /gene="mutH"
                     /locus_tag="OO339_000923"
     CDS             41880..42569
                     /gene="mutH"
                     /locus_tag="OO339_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5925647.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42638..43351
                     /locus_tag="OO339_000924"
     CDS             42638..43351
                     /locus_tag="OO339_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5925648.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43489..43707
                     /gene="ygdR"
                     /locus_tag="OO339_000925"
     CDS             43489..43707
                     /gene="ygdR"
                     /locus_tag="OO339_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5925649.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43815..44855
                     /locus_tag="OO339_000926"
     CDS             43815..44855
                     /locus_tag="OO339_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5925650.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44888..46078)
                     /gene="lplT"
                     /locus_tag="OO339_000927"
     CDS             complement(44888..46078)
                     /gene="lplT"
                     /locus_tag="OO339_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5925651.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46071..48230)
                     /gene="aas"
                     /locus_tag="OO339_000928"
     CDS             complement(46071..48230)
                     /gene="aas"
                     /locus_tag="OO339_000928"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5925652.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48816..49847
                     /gene="galR"
                     /locus_tag="OO339_000929"
     CDS             48816..49847
                     /gene="galR"
                     /locus_tag="OO339_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5925653.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49854..51116)
                     /gene="lysA"
                     /locus_tag="OO339_000930"
     CDS             complement(49854..51116)
                     /gene="lysA"
                     /locus_tag="OO339_000930"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5925654.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51238..52173
                     /gene="lysR"
                     /locus_tag="OO339_000931"
     CDS             51238..52173
                     /gene="lysR"
                     /locus_tag="OO339_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5925655.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52160..52852)
                     /gene="ygeA"
                     /locus_tag="OO339_000932"
     CDS             complement(52160..52852)
                     /gene="ygeA"
                     /locus_tag="OO339_000932"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5925656.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52981..54399)
                     /gene="araE"
                     /locus_tag="OO339_000933"
     CDS             complement(52981..54399)
                     /gene="araE"
                     /locus_tag="OO339_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5925657.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54714..55475)
                     /gene="kduD"
                     /locus_tag="OO339_000934"
     CDS             complement(54714..55475)
                     /gene="kduD"
                     /locus_tag="OO339_000934"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5925658.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55505..56341)
                     /gene="kduI"
                     /locus_tag="OO339_000935"
     CDS             complement(55505..56341)
                     /gene="kduI"
                     /locus_tag="OO339_000935"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5925659.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56628..57809)
                     /locus_tag="OO339_000936"
     CDS             complement(56628..57809)
                     /locus_tag="OO339_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5925660.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58064..59293
                     /locus_tag="OO339_000937"
                     /pseudo
     CDS             58064..59293
                     /locus_tag="OO339_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14784 a        16063 c        14573 g        14441 t
ORIGIN      
        1 ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa tgtttcttac
       61 caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg ttgcaacaca
      121 aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat tatcgccatg
      181 attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc cccggaagaa
      241 gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt ttgatttaca
      301 gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg atcaagatca
      361 ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg cggtaaatga
      421 ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc cgggctgaac
      481 tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac gactcaaacc
      541 agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac catgtggatg
      601 ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat caacgccggt
      661 gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat gacgtttttt
      721 gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga agacatcacc
      781 gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct gctctacttc
      841 ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac cgttgaaagc
      901 ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc gctgatcctg
      961 atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa agcgatgagt
     1021 attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct gatcccgcag
     1081 tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac cggaaacggt
     1141 ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa ccactctccg
     1201 atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc agaacagaaa
     1261 tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat gttcttcgtc
     1321 ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga gcagaacatc
     1381 tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg gatggctccg
     1441 attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg cgcacgtgaa
     1501 ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat cgaaatcaac
     1561 aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt tgccaccctg
     1621 aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc gatgatcctg
     1681 ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta cagcggtcac
     1741 atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat cttctactct
     1801 ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt ttaacgcgat
     1861 gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc ttccagttct
     1921 cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat tgcccgtaac
     1981 ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc tctgactggt
     2041 aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct gccggatacc
     2101 gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg tcgcctgatg
     2161 ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa cttccatgca
     2221 gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg cgataaagtc
     2281 gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga agagcatttt
     2341 ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc agccgatctg
     2401 caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa aaacgagctg
     2461 gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga agtcatgcgt
     2521 ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact gcgcgttcca
     2581 cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac cactgacccg
     2641 atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga gaacgctgct
     2701 ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc ggctgtgctg
     2761 gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg ttacctgctg
     2821 gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg tgccgaagtg
     2881 ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct ggcagaactg
     2941 ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga gcacaacctc
     3001 ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga gcgtaacgcc
     3061 attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac cagcgaaccg
     3121 cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga catgaacgcc
     3181 aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg cgattaatct
     3241 ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc atagcctgtt
     3301 ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg gctgttcatt
     3361 tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag ggttcgccct
     3421 gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag ccaacccacg
     3481 cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag cgtttaccag
     3541 attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg cctgcattac
     3601 gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac gaagccgatg
     3661 acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca acgattgttt
     3721 cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt gattacttcc
     3781 agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa ccgcagcagt
     3841 tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt gttgcgggaa
     3901 tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa gggatatatg
     3961 agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat aaagagatgg
     4021 cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac ggcaatttac
     4081 agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt atccggcatt
     4141 atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct caagcgttgc
     4201 ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca gtgccgaaat
     4261 gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat cacggttgag
     4321 ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac cttccacttt
     4381 cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag cgttatagcg
     4441 catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat aaaacgcgcc
     4501 cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc ccatacccgc
     4561 aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa ccgatcctcg
     4621 cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta gtgcccacgc
     4681 gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg tcgcggcttt
     4741 cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct gcgggatatc
     4801 atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca cgtagttaat
     4861 ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta cactgggttt
     4921 attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt tgttgctgat
     4981 aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga tcaggtaatc
     5041 cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg ttgggttggg
     5101 cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat cggtcacttt
     5161 cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct tctgataacc
     5221 acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc atgccgtttc
     5281 gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa attactcttc
     5341 aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt catcgctcag
     5401 cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca gcacttcaac
     5461 ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa gcccatgatg
     5521 ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag aaagttcgcg
     5581 tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg ccgcaatcat
     5641 gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat gaacggcatg
     5701 attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat ggaaacgcca
     5761 ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa agacaatatg
     5821 cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc catcctgata
     5881 acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca tttccaggca
     5941 agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct ctctgattca
     6001 aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa ttacccacca
     6061 tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac ctagctcaac
     6121 taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat gaccgctctt
     6181 aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg cccgttttcg
     6241 gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt tctgaaacgg
     6301 gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc cagctaataa
     6361 gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca tgagttcatt
     6421 tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc acttttgaaa
     6481 acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg aaacacatca
     6541 atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag aagttacatt
     6601 attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa taaccttaac
     6661 gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca ggctggcctg
     6721 atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg gatattgatg
     6781 aaaaaactca gttataaagc agggattatt accggattat ttttatatgc cttgggcgct
     6841 gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt tggcctattt
     6901 attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt tacggtatta
     6961 gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa ctcgtttggc
     7021 gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc acatcaatcg
     7081 caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca cagcctggta
     7141 ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt cgccctgctg
     7201 atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc caaacaagga
     7261 tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg ggcggtatta
     7321 gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat tcgctacgct
     7381 gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac cggaaccatg
     7441 gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt cgcaccacac
     7501 aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc agccttcgct
     7561 ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc gattcagtac
     7621 ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata cggttcgtcc
     7681 ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg ttttgtcagt
     7741 gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt cgcggtcatc
     7801 tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt tccgaataaa
     7861 gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc ccggttattg
     7921 acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat atggcgaaag
     7981 ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc gtcgagtgtg
     8041 tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa gaaaaattca
     8101 gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat ggcagtgaaa
     8161 ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc actgaacgcc
     8221 ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc atcccggcat
     8281 tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct gccgatgttg
     8341 aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg aaaggtaaaa
     8401 gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt gatcacaact
     8461 tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa ctgcgtcgcc
     8521 gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc tgggctgata
     8581 aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat gccgagcaaa
     8641 gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac atgatgcaag
     8701 gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac aacgccatcg
     8761 ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt gacaccgccg
     8821 aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt gtcgtggcga
     8881 ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc accggcaccg
     8941 ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag cgtgtaacgg
     9001 ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac tcaggttctg
     9061 ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg atgaagccgc
     9121 actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa tggtgcccgg
     9181 cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc gaaggcggcg
     9241 tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta ctgcaaatcg
     9301 cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac aaacgcacca
     9361 actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg ccgtttacgg
     9421 atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc atcggccacg
     9481 ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt atgcacaacg
     9541 ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg gatattgaag
     9601 gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa tacattacac
     9661 cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag tggttagccg
     9721 gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa gttatcctgg
     9781 tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag ggaaaaattg
     9841 ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac acctggcacc
     9901 agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca aatcaatagt
     9961 gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt ggatggcgct
    10021 ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg tccgcgaaca
    10081 gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca ggctatttct
    10141 ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa agaaaatcat
    10201 ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat taaccaccgt
    10261 ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat gctggatatc
    10321 cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg ccgactcttc
    10381 cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc cgcagcaatg
    10441 ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca gttcgccctt
    10501 tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg ggaaatttta
    10561 atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg ttccacctgc
    10621 gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc atccggcgtg
    10681 ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat gttgattgaa
    10741 gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga tttattgtcg
    10801 tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca tttctatcgc
    10861 gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct ggaaaaaatc
    10921 gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa cacattgtgg
    10981 aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga tgccgaaacg
    11041 accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa cagcccggaa
    11101 gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac tgaacctgaa
    11161 tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc ccgaactatt
    11221 gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg ctcactttcc
    11281 cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga gcgacttgct
    11341 ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg tgatgatggc
    11401 ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta atgcgctttc
    11461 actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt atgaacgggc
    11521 gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga atattctttt
    11581 aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg ggcggttatc
    11641 gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg acctgctgct
    11701 gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca gtaaagaaac
    11761 cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca atcatggacg
    11821 cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca tcaggctgaa
    11881 aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga ttgaagaagg
    11941 gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc agttgcctga
    12001 catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg gtaaacgcga
    12061 acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct gttacgtcaa
    12121 tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt tttcttgtga
    12181 agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg attacaaatc
    12241 gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta aatttaatcg
    12301 ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt ctgatgagcg
    12361 ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg gagacgacgc
    12421 cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca gatgcggcgg
    12481 acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat ctgagcattt
    12541 atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac ttcttcgtag
    12601 cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc attaaccagc
    12661 cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca taccatccag
    12721 gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca cgtcacctgc
    12781 ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac ccacatgttg
    12841 cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac cgcccacatc
    12901 ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa tgcctcttcc
    12961 agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg aatgcccata
    13021 tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc tggtgcaata
    13081 aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac cccagcatcg
    13141 cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa ctctttgctt
    13201 tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac accctgtaac
    13261 atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag ttcccccacc
    13321 acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat taacttatcg
    13381 ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt cacgcgggca
    13441 aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca ctctttttca
    13501 aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc gctttaaacg
    13561 gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc caaccggtgt
    13621 gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc cgctgaacag
    13681 caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga tgcgacgctg
    13741 cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt cgagaccggt
    13801 ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg caccaaaagc
    13861 gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa aacggctggt
    13921 catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt cttgttcagc
    13981 agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt ctgcctggct
    14041 gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa acgggcaaac
    14101 aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg ccactccctg
    14161 cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc cttgctgaac
    14221 gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc gtgacgcatc
    14281 atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt cgcctgtcag
    14341 cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca cccgtagccc
    14401 cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca gcttatcttc
    14461 ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat taaagctgga
    14521 aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg tcaacgcccc
    14581 cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa atatctcttt
    14641 gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat tacggcggcg
    14701 gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta ctgcggcctg
    14761 ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg cggcatcaaa
    14821 aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac tttgatgtta
    14881 aatgaattta acaattagat cacactgtgt aacctattag tttttttaat ctgagccatt
    14941 ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact tcctgagccg
    15001 gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg gttgtgatgt
    15061 tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag ctaccttttt
    15121 tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc gcctaattgc
    15181 ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat ctctttcacg
    15241 tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat cataccgata
    15301 tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt ggtgtgcatc
    15361 acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc agaaataatt
    15421 gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa tagtattgat
    15481 taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc ggcttcgcca
    15541 ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa ttttgcgtca
    15601 cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc agtttcgcgc
    15661 ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga ccgcgcttaa
    15721 acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg gaaacgtcca
    15781 tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg cacgagagaa
    15841 agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc gcggcaccac
    15901 tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac cgggcgatga
    15961 gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga tggtcgccca
    16021 acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc cggatgtcga
    16081 tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc agatgtcgaa
    16141 cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt cagccgggat
    16201 ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg ttcagcaact
    16261 ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg gtatcggcgt
    16321 gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg gcggcaaaat
    16381 gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga aactggaagc
    16441 aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat ggctggcaga
    16501 ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg cagaagaagc
    16561 gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc tgctggcctt
    16621 tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt acggtattgc
    16681 cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg taaccggcac
    16741 actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc tggtgaatgc
    16801 cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt cgccggacat
    16861 ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc gttgtcgcaa
    16921 tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc tttgccagac
    16981 gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg gctgggatat
    17041 accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg cattgtgcgc
    17101 ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga attacaggca
    17161 cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag cgcctcacgc
    17221 agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca ggtttaagca
    17281 cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta accctgacgc
    17341 gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc aaaggtggcg
    17401 gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg cccttctgcc
    17461 gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc ttcagtagaa
    17521 aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa atcgcttttc
    17581 aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag atcggtaacc
    17641 tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag cgggatttta
    17701 ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat ggcatcaatc
    17761 acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac gaaatcatcc
    17821 accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat gacttccgct
    17881 tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt ggtgacgcac
    17941 acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag cgcttccgcc
    18001 gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc aaacagttgc
    18061 aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca tgcatcacta
    18121 attaccacag acatagcacc tcagaattaa aaagggcgaa gttatccctc accctaaacg
    18181 cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat attttggatt
    18241 tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac aacgcgcact
    18301 tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa aacggcatac
    18361 agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt cagcacccaa
    18421 acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc gatcccttga
    18481 tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac catcagacgc
    18541 aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc tgccagcaag
    18601 gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc aaccagcggc
    18661 accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac gaaagacggg
    18721 ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca atgacgaatc
    18781 gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac cttaccaatg
    18841 ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg actgccatca
    18901 ccaaagtcga tatacccact accctgcaca tccataatga agttatccat cagagagttg
    18961 ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc acgagacggc
    19021 agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc ctggcgggta
    19081 tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc atagttgtcg
    19141 gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt atcaccgcct
    19201 gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata cagacgcggt
    19261 gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat cggcgcgcca
    19321 acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc tttatattgc
    19381 tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac cgtgcccata
    19441 agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa aaataaggcg
    19501 caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct gacgcaaatt
    19561 ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta aatccgtgat
    19621 cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat ccacggacgc
    19681 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    19741 ggcccccgca accaatcaaa tttgatgaag taaaagcagt acggtgacgc ggggtggagc
    19801 agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc ggcccccgca
    19861 accaattatt gaacacccta acgggtgttt ttttgtttct gatctcccat aaaaaaagcg
    19921 ccattcagcg cctttttatc atccccttct cgccagcgtt gccccatcgg caaaatacgc
    19981 tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg ttttcagttt
    20041 acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg gaatatctgg
    20101 tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct tgttgatttt
    20161 tccgagctta ttcagtaccg ctttaccaaa cttaaggctg tcagcaatag tcagcgactg
    20221 caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca cgccaccaat
    20281 caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg ttgcgccttt
    20341 ggttgagagc gcaaacaccg aggaaccgct cggctgacga ctggtaaagg cgtcggcatg
    20401 gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca cttgcaacgg
    20461 aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt cctcgatcag
    20521 agagcgcaaa cggcgagcta tttgcaatac cacatctttt tcgcgcgttt tgtatttccc
    20581 caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa tcggacgatc
    20641 gcgccctgct ttacccggtt gtggaccact ttgcgctggc ggcacctgct tttcgaggtc
    20701 gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct gtgcattggc
    20761 cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa gggcaaacag
    20821 ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt acggtttgcg ggtcaaattg
    20881 cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg ccatcccctt
    20941 gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttcag ggttactcaa
    21001 cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg tgtagctgga
    21061 cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga cctgacttac
    21121 gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga ttgcagtgtt
    21181 ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa gtaaacgact
    21241 aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa cggtaaacat
    21301 tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa tttacacttg
    21361 caccctgcga aaaaacagaa taaaaataca ataatttcaa ataatcatgc aaaggggtgt
    21421 gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc ggttccctat
    21481 atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc cattgagcat
    21541 gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg catccgtctg
    21601 gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca tcaccacgaa
    21661 ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact ggtgaagcag
    21721 gctgcgggaa cattgcaact ggatattact gctcgcctgt caatgagtct caataacacg
    21781 ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt ttattattgc ccagccgctg
    21841 ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat tgatgaagac
    21901 gcgctccatc gtcaactgga aagcggtgca atagtgctaa tggggccggt cgctgtttca
    21961 gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact agccatcaaa
    22021 ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgttactaa tgacgacggt
    22081 gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc ccaggaagag
    22141 aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa agcctgccgc
    22201 agcggcgtgc gccgctgtca tttaatcagt tatcaggaag atggcgcgct gttgcaagag
    22261 ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga gcagattcgt
    22321 cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct ggagcagcaa
    22381 ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa attcaccatt
    22441 attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc ggaagagaag
    22501 attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc acggggcgag
    22561 gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa attgtttgtg
    22621 ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt ggatattgat
    22681 ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa agtgttgatg
    22741 gcggatttag ggtaagcgat gaaaatcacc ggatgcggca ggcgtaacac tcgtgcgtcg
    22801 catccggcaa ttacgtttat tcccgcgaac taaacaacgc cgccagacca ctgcgccgct
    22861 cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc gacagacgac
    22921 gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactaccggc gtgcgttggc
    22981 tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt accgtcatcg
    23041 cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata ttgccgtccg
    23101 gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg atatcgccat
    23161 taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcggcct tcgtaccaac
    23221 gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgttca attcgctcat
    23281 tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg tactcattga
    23341 acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga taacgtccgt
    23401 aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt aaaagtcgtt
    23461 tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta tcaccacggt
    23521 tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag cttttttgca
    23581 gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc ggaacgtgag
    23641 tccccgtcag gcggcttagc tgcctggcac gctcggcggt aaagcccgcg ttggcataag
    23701 cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca cgatcgccga
    23761 gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag tctcgacatc ataggcagat
    23821 cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta ccggcatgat
    23881 gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg ctggcatctt
    23941 ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc ttgccgagag
    24001 attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata cggcagcgtt
    24061 cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc gttttaccgg
    24121 tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc gctgcaactt
    24181 tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc gccagtagag
    24241 cttcatcaac ctcaatggta tgattcacct cattgaagaa gcgcgctacc gtccgctcgt
    24301 tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc gtgggttcat
    24361 ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt agctcaccga
    24421 tttcactgac acaggtcgcc aacagcggat tcgacgcctc gttattttcc agtcgtgaaa
    24481 gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc gcgagggtga
    24541 cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc gggcgtagct
    24601 gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct cctccagggt
    24661 cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc gggttgtgta
    24721 aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga taacgccgcc
    24781 aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc agataacgat gcagcgccag
    24841 ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg ccatagcctg
    24901 ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga gcaggtaata
    24961 acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc gtacctgcat
    25021 gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta gtgcatcaag
    25081 ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca cctgtttatc
    25141 gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct ggaggacagc
    25201 cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga gttccagttt
    25261 ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa acaaactgtg
    25321 caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg tcggttcttc
    25381 aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat cctgagcgat
    25441 accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat cgccgggcaa
    25501 tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct gccagggctg
    25561 gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg cttcaataca
    25621 ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc cgagcgcact
    25681 ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg gtgcaacgcc
    25741 gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca gatcttccgc
    25801 cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcattaagat ccaaaactgc
    25861 ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat tggtaataaa
    25921 tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga caatctgcac
    25981 cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt ctggctcgag
    26041 gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg ttccggcttc
    26101 ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc ctgccgttgc
    26161 cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg gcataacgcc
    26221 acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat cccacgcatg
    26281 ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg acgttgccag
    26341 cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca accaaagcat
    26401 ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa cggaagggat
    26461 ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc gcaccagcac
    26521 gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca acgcttcgcc
    26581 ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg ccatggtact
    26641 ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca tcgcaggctg
    26701 cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc ccggctgatt tcactggaat
    26761 aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg ctgaaaagct tgttcacact
    26821 gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg catgaacttc
    26881 actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata tggcctgctt
    26941 cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac ggcgaaaaat
    27001 tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg ggaatcgcgt
    27061 acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat cgagccgact
    27121 taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta ctgtttcgcg
    27181 gatctcagcc aatgcgcggg tgatcaacag atcgcggatc gacaatggtt ctgcaagcag
    27241 ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggcct tcgtgcgatc
    27301 ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac tgtttgtctc
    27361 ttcttctgcc caggcgctga tcttttcgat ccatttagcc tgattgctac ggttaaactt
    27421 gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg cgtcgcgcca
    27481 ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag ccagcgtttc
    27541 atcatcgggc ggcggtgctt tgataaccgg cggttcgcct tgcagataac gattaatatc
    27601 gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta tttcacgcgg
    27661 cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta gcagagactc
    27721 atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca ggttgagcat
    27781 gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca tccatctgcc gttcggctaa
    27841 caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc gttcgtacag
    27901 tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat tgctacggat
    27961 acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca gttcttcaac
    28021 gatcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc gcaaatagag
    28081 cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc gctcgccctg
    28141 taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt tcactcatca
    28201 ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct tcaggatgca
    28261 catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc atgccttgcg
    28321 gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc agttttatct
    28381 gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa tctttactta
    28441 acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta attaccgcct
    28501 gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct gcggttggga
    28561 aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt tgcaaaagga
    28621 agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca tagcccaatt
    28681 gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc aacagagagc
    28741 tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct gccagtgcgg
    28801 agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg accactacat
    28861 ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca tcgcgtgcca
    28921 gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt cgagcccctg
    28981 attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc aaaattttac
    29041 gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc ataatcgcct
    29101 gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag gacttcgcct
    29161 gctcaagctg ctcttccgta gcggtatagc taaagtaccc ctcgagcaat gcctggaaca
    29221 gctgcggcag acgctgggtg taaccattag cattaatcat aaggccgttg ttagcgttgg
    29281 tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc gccagccctg
    29341 cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg gctttcggat
    29401 tacgcaaaat caggctgaca tcagctttgg gctcgctggc aaaataacgg cttggcgcat
    29461 acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcgtat ttcttctctg
    29521 acttaatcag cgagaaatca tcaggaatat aagggttaag ctctggcaaa gagagcgcaa
    29581 tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta tcgacctgat
    29641 acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg atataccaga
    29701 tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta gcatcgtacc
    29761 gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc atggtatctg
    29821 ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag tcgatatcca
    29881 gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt aacagattga
    29941 gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta tcagttaaag
    30001 acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag ttggcgctaa
    30061 tgccctcaac taatccctgc ttttgcagcc agtcagaaag tgtacctggg ctgcgattgc
    30121 caatcagata ggtaatcaat tcatcggttt tactacggaa cttcgctgag ttgttatcga
    30181 tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg gacgtaatga atgataatgc
    30241 ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc tctttgttcg
    30301 gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt ttattactgt
    30361 aaataaccgc cttcatcaaa ttggcggaat agtacttctc gtggaaatct ttcagcgcct
    30421 gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca gaaaactttg
    30481 aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc atcccgtcac
    30541 gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg gcgtatttct
    30601 tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc taccgcaccg ggcaaggcgt
    30661 cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca ttgtgactac
    30721 cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt ttcgacccca
    30781 tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga tcttccagcg
    30841 acccaacggg cactaccagc gccgagagcg atttaactgc ctgcggatca gaaaccagca
    30901 agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct ttatcacttt
    30961 tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaat ggtgcccaga
    31021 gggcaacaaa caacaataat gctttgaacc aggtgctgcg gggcattcac ggacctcata
    31081 atcaacttta ttttctggac agattcaaca ggttaacgca aatcatctgc cagaatttaa
    31141 tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac tggtgcgcat
    31201 cataaagtaa gcagatagat tgcgcaattt ttatacaaca ctcatgactg attaaagcga
    31261 aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt ctctggtgtt
    31321 aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac catcatgttg
    31381 ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc gtcatccagc
    31441 atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact ttcaggtaac
    31501 accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga gaggtaatgc
    31561 aaagcctgtt cggctgcaag cggcggaaaa cgccactcac cgtctttgcg tagaaaaagg
    31621 cgactttcgc cattaccacc gctggcacag tagacaaggt gttccagcca aagttgcatt
    31681 ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg ctgcacctgc
    31741 ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat actctgcccc
    31801 ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca ctgtgtttcc
    31861 cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa gcggcggaac
    31921 aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt gatttgataa
    31981 cggctaagtc cttccagaat aaatggctcg gtgtctggga tttcgctgtc ttcagtatgg
    32041 aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca gaatcgttgt
    32101 agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac aaattcagaa
    32161 tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct ttgtcgttcg
    32221 cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt aagatgcgct
    32281 tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag ataatgactt
    32341 tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc actgttatcc
    32401 tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat taacgcttcc
    32461 aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg tttctggctc
    32521 atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt cattcccagc
    32581 aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat gttaaccggt
    32641 ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag ttcatcacgc
    32701 aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc gataatcgcc
    32761 tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg caggaagaag
    32821 gcgttgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc ctgtgccagc
    32881 ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac cagttctgca
    32941 attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg cgcgctctcc
    33001 atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg tccggtggcg
    33061 gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc ggattcgttg
    33121 acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc cgccagcacc
    33181 ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg cagtgataac
    33241 aggctgataa acgcttccag taccggatgt gactgccgcg cacgacggtc ggaaatggcg
    33301 taaggcaggt aacgatccgc aggtgcgcta ccaaacactg cctgaataaa cggactgtag
    33361 ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg ggcttcctcc
    33421 agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac gctgcgggct atggcaaacg
    33481 tggaatgtga tactgctatc cagtggatca agcgggcgtt tgttatcgct acgggaaaac
    33541 tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt ccagaatgtc agactgaatg
    33601 ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt ccagctcctg gctgctctcc
    33661 aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc cccatgaggc cagcagcgag
    33721 ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc gctatcgcga
    33781 aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgagtcag cagtttcgcc
    33841 agataagcag gatctttaat atcgccccag taataacggc aggggttggt aaacaggaga
    33901 tggatttcaa tatgtttacc cagcgcctgg agcgcctgga gataaacagg cggtaacgcg
    33961 gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt cgcggactcc
    34021 agcgtttcga taaagcgctg atagagattc gcgcggtgcc agcgcggttg tccgagttca
    34081 tgggtatatt ccaccagcgc cttccacaac ggggcttgcc aggcctgtgc ttctcccagc
    34141 ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata gaccagatac
    34201 tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt gtcgctatcg
    34261 tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccagcaattg cggcagcaga
    34321 gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg gatttccggt
    34381 aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc aatgtttgcc
    34441 gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc ggtactttgc
    34501 accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac aataaactcc
    34561 atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat agcggctcct
    34621 gactactgac aattcgggca atgcaggcgg gtcatctcgc cttctctgcc cccgggtgaa
    34681 actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg gttgacctgc
    34741 cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg ccgccagagc
    34801 ttttggtact ggtttctgct ggcgagactg ttcattgatg ttcgctggat acccgataac
    34861 gcagtgacaa tcatcaccat cagcaccatc agcaccatca gcaccatcag caccatcagc
    34921 accatcgcaa ccattacctc cggcaggcta aagccttgtt gattcttcag ggaagctgac
    34981 ataatgcccc ctctttcagc ggacaaaaat cgctccagcc gcgtggcgaa aaaacaattt
    35041 ttccatcaat gacttcgcct gttcgccaca acgaaacgcc ttcataaccg gcaatcaata
    35101 aggcttcatt atctgccagt aaacgcaaac aaacctgggc atcggttcca gcgtactgcg
    35161 agcactgaac tgctggctgc gtctgccagg agtgcatttt tccccacgcc agcgccgact
    35221 gaacgatggc ctggcggcgc aatgactgac tttccatgct cacgcgagag gcaaaactgc
    35281 gatcctgctg actcattcct tgcaatagca agctacccaa aaccagcagc atcaggacca
    35341 gagccagtga cgaaacacct ttttcgcggt tcacaggttg aatcctgtca cgctatagct
    35401 ggcatccacc acggtttgcg gttcagactt actggcagca cgcatattaa ccgtcaacac
    35461 cggcgagaag ccgctgacat cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc
    35521 cggattagtg actttatccc agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc
    35581 cagcacatgc tccttcagac gaaatccaat ctggtcggac tcttttaccg gttcgcgatc
    35641 ccagataccg ttactgttcg catcccactg cacaatgata cagtcaccct gtccgacaat
    35701 ttccagccct tcgccggtac agctgccatg acaataaccc gccctctgga gatgcttcgc
    35761 gacggtaaat acccgcagcc agatttcatc ttccagtgcc agcttacgag tgctcgttaa
    35821 actttcacgc tgtaacgcag gcagaaagcg tgcagccccc agcaacaata cgctactgat
    35881 cgccatagca atcaacactt ccagcagaga aaaaccttgc tcttttacag gcatccttct
    35941 gtttctcctt gctgataaag ccggagtctt ccccacggcg aaaccaccag ccaccactcg
    36001 cccgttgagt ttttgaagcg aatatgcacg gcccatgcgg tattgcgcag gccaaagaaa
    36061 gcaagcgaag gtgtcaggtc gctcatttcg acttcgggcc agcgtggcac aaagaccaat
    36121 ggtgaactgc catgacaggt attggcccca gcagcggaac tcacaaggca ccataacgtc
    36181 ccctccctga taacgctgat actgtggtcg cggttatgcc agttggcatc ttcacgtaaa
    36241 tagagcaaat agtcccgcgc ctagctggcg gtttgccaaa gccgttgcga ctgctgccag
    36301 tattgccagc catagagtcc acttgcgctt agcatgacca aaatcagcat cgcgaccagc
    36361 gtttcaatca gcgtataacc acgttgtgtt ttcatgccgg cagtatggag cgaggagaaa
    36421 aaaagacgag ggccagtttc tatttcttcg gcgcatcttc cggactattt acgccgttgc
    36481 aggacgttgc aaaatttcgg gaaggcgtct cgaagaattt aacggagggt aaaaaaaccg
    36541 acgcacactg gcgtcggctc tggcaggatg tttcgtaatt agatagccac cggcgcttta
    36601 acgcccggat gtggatcgta gccttcaatc tcaaagtctt cgaaacggta gtcgaagatg
    36661 gattcgggtt tacgtttgat aatcaacttc ggcagcggac gcggttcgcg gcttaattgc
    36721 agatgagttt gatccatatg gttgctgtac aggtgcgtat cgccaccggt ccagacaaaa
    36781 tcacccactt ccagatcgca ctgctgcgcc atcatatgca ccagtaacgc gtagctggca
    36841 atgttgaacg gcaggccgag gaagacgtca caggagcgct gataaagctg gcaagagagt
    36901 ttgccgtctg ccacatagaa ctggaagaat gcatggcacg gtgccagcgc cattttatcc
    36961 agttcgccta cgttccacgc tgaaacaata atgcggcgcg aatccgggtc gtttttcagc
    37021 tggttcagta ccgtagtaat ctggtcaata tgacgaccat ctggcgttgg ccaggcgcgc
    37081 cactgtttac catacactgg cccgaggtcg ccattttcat cggcccattc gtcccagatg
    37141 gtgacattgt tttcgtgtag ataagcaatg ttagtatcgc cctgcaggaa ccacagcagt
    37201 tcgtggatga tggaacgcag gtggcaacgt ttggttgtca ccagcgggaa tccatcctgc
    37261 aggttaaaac gcatctgatg accaaaaatg gaaagcgttc cggttccggt acggtcgttt
    37321 ttctgtgtgc cttcgtcgag cactttttgc atcagttcta aatactgttt catggttcct
    37381 caggaaacgt gttgctgtgg gctgcgacga tatgcccaga ccatcatgat cacacccgcg
    37441 acaatcatcg ggatggaaag aatttgcccc atgctgatgt actgcaccca ggcaccggta
    37501 aactgcgcgt cgggctggcg gaaaaactca acaatgatgc gaaacgcgcc gtagccaatc
    37561 aggaacaagc ctgagacagc tcccattggg cgcggtttac gaatatacag gttgaggata
    37621 ataaacagca ccacaccttc cagcagcagt tcgtaaagct gtgatgggtg gcgcggcagc
    37681 acaccgtaag tgtcgaaaat ggattgccac tgcgggttgg tttgcagcag caaaatatct
    37741 tctgtacggg agcctgggaa cagcatggca aacgggaagt tcgggtcaac gcggccccac
    37801 aattcaccgt taataaagtt gcccagacgc ccggcaccaa gaccaaacgg aatgagtggt
    37861 gcgataaaat cagagacctg gaagaaggaa cgtttagtac ggcgggcgaa gataatcatc
    37921 accacgataa cgccaatcag gccgccgtgg aaagacatgc cgccgtccca gacacggaac
    37981 agatacagcg gatcggccat aaactgcggg aaattgtaga acagaacata accaatacgt
    38041 cccccgagga agacgccgag gaagcccgca tagagtaagt tttcaacttc atttttggtc
    38101 cagccactgc ccggacgatt cgcccgacgt gttgccagcc acattgcaaa aatgaacccc
    38161 accagataca tcaggccgta ccagtgaagc gccacgggtc ctattgagaa aatgaccgga
    38221 tcaaactccg gaaaatgcag atagctactg gtcatctgtc accacaagtt cttgttattt
    38281 cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt tcaggcgctc caaaggtgcg
    38341 aataatagca caaggggacc tggctggttg ccggataccg ttaaaagata tgtatatgat
    38401 ccgcgctata accctccgcg aatcagcccg cccatgccgc gacgctccat aaaggctgca
    38461 acctgatgac gaacttcggt cgccagttgc gcttccagac tacgctgcgc aagattttct
    38521 gcttcggcaa aatcaatgcg ccgcagcagg tattttaccc gcgctacaga acgtccgttc
    38581 atagacagat ggcgataccc aagcccaatg aggattgcca cgcacatggg atcgcccgcc
    38641 atttcaccgc acaaacgaag atcgatacca tgtatttccg cttcccgggc gatcatcgcc
    38701 agagctcgta acattgcagg atgaagactg tcgtaaatgt tcgccacccg ggtattgttg
    38761 cgatcaacgg ccagaatgta ttgagtcaga tcgttggtgc caacagagat gaaatcgacc
    38821 ctctttgcca gatgcggcag cataaatacc attgacggca cttccagcat gatgccgata
    38881 cgtggtttgg gaatttcgta accgatcatc tcctcgactt cacgtccggc acgttcaatc
    38941 aggcggcgtg cttcgtcaac ttcatcgagg ctggtgacca tcggcaacag aatattcagg
    39001 ttgcccgtag cggcattagc acgcagcatc gcccgcacct ggatcaagaa gatctccggc
    39061 tgatcgagcg taatgcgaat cccacgccaa cccaggcatg gattctcttc gctgatcggc
    39121 atgtaaggca gctgcttatc tgctccgaca tccagcgtac gcaaggtgac gggtttatca
    39181 ttgaacattt gcagcatccc ctgatactgc gccacctgtt cttcttccga cgggaaacca
    39241 ctttgcagca tgaatgggat ttcagtgcga taaagaccga tgccatcaat acagctgccc
    39301 agtttttctt catgttccgg gcttaaacca gcattgagca tgacttttat acgctcaccg
    39361 ctttttaact gcgcgggtaa attgacgtca tcttccgcca gacggctaag ctcaatctct
    39421 tcactaatta gccgctgata ttcttgcagc agtaccggct ccggatcgac cagcaattca
    39481 ccgcgatagc catcaacgat cagcgtccta cgatgcagca ccgaaggctg aatatccgcg
    39541 cccatcacgg tagggatccc cagcgcacgt accatgatcg cagcatggga gttggctgcg
    39601 ccatctcgca cgacaacacc gactaagcga tcctggggca gctcagcaag agttgtcgct
    39661 gacagttcat ctgccaccag aatgaaacgt tccggccagg cgttcggccc ttgattagcg
    39721 tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta aatcaccagc ccgctctttg
    39781 agatagttat cgcttagcgc ggcaaactgt tcggcaaatt tttcaatgac cgtttttacc
    39841 gcccactctg ccaccgagcc tttatcaacc tcggcaaaca attcgcgacg cagccgggtg
    39901 tccgaaagca ggtgcgagta aagatcgaaa atagccgccg tttctttttg tgctccggcg
    39961 gcaaagcgtt tgctgtagcg gcgaaactcg tttgccgctt cttccagcgc cccggtcagt
    40021 cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat acacctgttc cattaaaggt
    40081 aacgtggcat cctgccagcc ttcggcaatc gccacaccag gtgctgccgg taatgcgcgg
    40141 attcgcgtct ggcgatattg cccaaacagg gcagtcaact gcgactgaga aagaatagct
    40201 gccatctggg tggcaagcgt caccaggaag gattcttcac tttcgtcata ctggcgcaac
    40261 tctcgttgct gtaccaccag tacaccaagc aactggcgac gttgaataat tggtacgcct
    40321 aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt atttgaagct ggggtgcttt
    40381 tgcgcatctg caaggtttat cggttccgcc agcctgccaa ccaggccgac gatcccttca
    40441 tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca gcccccgggt cgccatcagg
    40501 tagtaacaac gtcgatcatg atcggccagg tagaccgaac agacctcggt atccatcgca
    40561 agacagatgt cggtaaccag aatatttaac gcctcattca ggcgtggtgc gctggctacc
    40621 ttttcgacta tttcgcgcag gcgagtgagc ataattggcg tgacttaacc tcttttacgt
    40681 cgataagcag atgcgttttg tggttttggc gtattttcct gcagtgacat caccacactc
    40741 gcgaactctt tcattaccct acggtagaca tcacgtttaa atgacaccac ctgtctgacc
    40801 ggataccagt aacttaccca tcgccagccg tcaaactctg gtgtactgct ggtttgcata
    40861 ttgatttctg catcgccgct taccagctgc aagagaaacc atttttgttt ttggccgata
    40921 caaaccggct tcgtgtccca acgcaccaaa cgtttcggta atttgtagcg caaccagtta
    40981 cgcgttgaag caaggattcg aacgtctttg cggcttaatc ctacttcttc aaacaattca
    41041 cggtacatcg cctgctctgc ggattctccg gggttgattc cgccttgcgg aaattgccag
    41101 gagtgctgac caaatcgccg ggcccacatt acctgcccct ggcgattaca aatcacgata
    41161 cctacgtttg ggcggtagcc atcgtcatca atcaccggac tacctcaaaa taaagcttta
    41221 tatacgaatg attgtttcat actccaggaa gacggtaaac cactctctgc agggcattac
    41281 acactaataa caattgaata actcacagtt atgtgcagag ttataaacag aggaaggggt
    41341 ggatagccgt ttttattcac cttttctgtg gatagagttg tgaagaacta cggaattact
    41401 acgggaaaac ccggagcatt ctgaataagc ccgatataga cattttaaaa tattcattat
    41461 tagtcaatgc attacatgta atttgagtaa tgaaaaagag taattcgtga cccaggtcac
    41521 acctctcatt tacgggttgg cgaaagatca accaatgccg tgtttatcca cagaatgtgc
    41581 cactaagtta agcactgaac cactaaaaac tggagtttcg tcgcacgtca aggctgtaaa
    41641 tgaaaacagt agtggaggtt tttcacagtt atcccagctt tctgtggata acatggtgta
    41701 agatcctgtt tattttcagt gaccagattt ggaaaaccta ttgcagtgtt gcgcaactcg
    41761 attaccggca gcctaaaaag caatataaat cagagaattg aacaacacat gtggaaaaag
    41821 ttacactgcg aatattcggc acataattgc tgtttgtttt ttaaccaagg tatcatgaca
    41881 tgtcccaacc tcgcccactg ctctctcctc ccgaaactga agaacagttg ttagcgcaag
    41941 cacagcaact ttctggttat acattgggag aactggcggc acttgacggg ctggttacgc
    42001 cagagaattt aaaacgcgat aagggctgga ttggcgtgtt actggagatc tggctaggtg
    42061 ccagcgcagg gagtaaacct gagcaagatt ttgctgctct gggcgtggaa cttaaaacta
    42121 tccctgtgga tagtcttggt cgtccgctgg aaacaacatt cgtttgtgtt gcccggttaa
    42181 cgggtaatag cggggtgacc tgggaaacca gccatgtgcg ccacaagctc aaacgcgtac
    42241 tgtggatacc ggttgaaggc gagcgcagca tcccgctggc gcagcgtcgc gtaggatcac
    42301 cgttgctgtg gagcccgaat gaagaggaag accgacagct acgcgaagac tgggaagaat
    42361 taatggatat gattgttctc ggtcaggttg agcggatcac cgctcgtcac ggggaatatt
    42421 tacagataag accgaaagca gcgaatgcga aagcgcttac cgaagccatt ggtgcccggg
    42481 gcgaacggat tctgacgctg ccacgcggct tttatttgaa gaagaatttc accagtgcac
    42541 tactggcccg tcattttctg atccagtagc catcgctttg acctgccgct ttccgggcat
    42601 ataattaccg cttcattttt ttggcagggc tttttagatg ttatttgcat ggataaccga
    42661 tcctaacgct tggcttgcgc tcggtacgct gacgctgctg gagatcgttc ttgggatcga
    42721 caatattatt ttcctttctc tggtggtggc aaagcttccc acagcacaac gtgctcatgc
    42781 gcgccgtctg gggttggcgg gagccatggt tatgcgtctg gcgctgctgg catcaatctc
    42841 ttgggttacg cgcctgacaa atccgctttt tacaatattc agtcaggaaa tttctgcccg
    42901 tgatttgatt ctgcttctgg gtggcttgtt ccttatctgg aaagccagca aggaaatcca
    42961 cgaatccatt gaaggtgaag aagaagggct gaaaacacgc gtttcatcat tcctcggcgc
    43021 tatcgtgcag attatgctgc tggatattat cttcagcctc gactcggtga ttaccgctgt
    43081 gggtctgtca gatcacctgt ttattatgat ggcagccgtg gtgattgccg taggcgtgat
    43141 gatgttcgcc gcgcgctcga ttggtgattt tgtcgaacgc catccttcgg taaaaatgct
    43201 ggcgctctct ttcctgattc tggtgggctt taccctgatt ctggaaagtt tcgacatcca
    43261 cgtaccgaaa ggttacatct acttcgcgat gttcttctct attgcggttg aaagcctcaa
    43321 cttgattcgc aacaaaaaga atccgctctg atactccgta cgctctcctg cgggagggcg
    43381 tactcactcc ttttaccctc acagtttaag attttcctgc tttcaagata tataacgtcg
    43441 gtttataaac agactattat cataggtagg ccaaatgagg ataaccggat gaaaaaatgg
    43501 gcagtaataa tttctgcagt cggactggcg tttgctgttt ccgggtgttc cagtgattac
    43561 gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg gaaaacctga aattgatgat
    43621 gataccgggc tggtgagtta tcacgatcag caaggtaacg cgatgcaaat taaccgtgat
    43681 gatgtttcgc aaattattga acgttaacaa ataaggtcag catccggctg gccttaagat
    43741 ttttctcttt cccttttccc ttccctctgc catttttata ttccttatgt cgtgattata
    43801 aaaaggaaac ggctatgcaa tatcaccgta taccccacag ttcgctggaa gtcagcacgc
    43861 tggggcttgg cacgatgacg tttggtgaac agaacagcga agccgacgcc cacgcacaac
    43921 tcgactatgc cgtcgctcag ggcattaacc ttatcgacgt tgccgaaatg tacccagtac
    43981 ctccgcgccc cgaaacgcaa gggttaaccg aaacctacgt cggcaactgg ctggcgaaac
    44041 atggcagccg cgaaaagtta attatcgcct ccaaagtgag cggaccgtcg cgcaataatg
    44101 acaagggcat ccgcccggat caggcgctgg atcggaagaa tatccgcgaa gcgctgcatg
    44161 acagcctcaa gcgcctacag actgattacc tcgatcttta tcaggtgcac tggccgcagc
    44221 gcccgaccaa ctgcttcggc aaactcggtt atagctggac ggattctgcg cctgcggttt
    44281 cgctgctgga tacgctggac gcactggcag agtaccaacg cgcgggaaaa attcgttata
    44341 tcggcgtgtc gaacgaaact gcatttggcg taatgcgcta cctgcatctg gcggacaaac
    44401 acgatctgcc gcgtattgtc accattcaga acccttacag tctgttaaac cgcagttttg
    44461 aagtaggtct ggcagaagtc agccagtatg aaggggtcga actgctggcc tattcgtgcc
    44521 tgggtttcgg cacgctgacc gggaaatatc tcaatggtgc aaaacccgct ggcgcacgta
    44581 atacgctctt tagtcggttc acccgctata gcggtgagca aacgcaaaaa gccgtcgcgg
    44641 cgtatgttga tatcgccaga cgtcatggcc ttgatcctgc tcagatggcg ctcgcgtttg
    44701 tacgccgtca accgtttgtt gccagcactc tgctgggcgc aaccacgatg gatcagctga
    44761 aaactaacat cgaaagtttg catctggagt taagcgaaga cgtattagct gaaattgaag
    44821 cggtacatca ggtttatact tatcctgcac cataaagatt aacgccggtt aaaaccggcg
    44881 ttaaatatta atgacggcgc tgccagatcc acagcgccgt tattgccagc gcaaatagcg
    44941 caccgaagcc aatgccaatg ggcacgaccg ggatgcctac cattaccgcc agcgagtaaa
    45001 tgcccagcat caacaacatg gcgctgtttt cgccaaggtt ttgtactgca atcgcattcc
    45061 ccgccccgac gcttttttta ccccgctcct gtagcaacgc attgagcgga acgacaaaaa
    45121 agccccccag cacgccaatc agcatcaaca aggcataggc tggcagcagc tcgtgttgca
    45181 gggagaaaat cggtaccacc acgccaatca aaatcccggc tggcatacag cgtgacacgg
    45241 tttccagcgt aaccagcttc gctgccgcac ctgcgccaac cacgatacca atcgctacca
    45301 tcgcgttgag ataggtcggc gtagcgttat cggtaatgcc cagcgccacc ggtacccaca
    45361 gcaccaacag gaaacgcagc gtgacacccg ctccccagaa taaactggtg cccaccagcg
    45421 aaaaacgcgt ttcaccattg caccatagcg aggtgcaggc attcaggaaa ctgcgggtca
    45481 tgttgatgag attccaggac tgccccggac gcgccgccag tttgggaatg tagatattgg
    45541 caacgaccgc accaccgtag gccagtgcgc atgcggccag agcgaccagg acatgccagt
    45601 cagccagcac accaccggct acggaaccga gtaaaatcgc cgctatggca gaagcttcca
    45661 ttaaaccgtt agcttttact aacttactac ccgtggttaa ttcgccgaga ataccgtatt
    45721 tcgccggtga ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga
    45781 taccaaagca gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc
    45841 ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgccaaaaga atgtaagcac
    45901 ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta
    45961 acgccagagt ggcaaacagt agcgcgttat cgccaaacgc agagagaaac tgcgccacga
    46021 taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg
    46081 tgttgttccg cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca
    46141 agtaatggca tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg
    46201 tgctcgcggg catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc
    46261 accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc
    46321 gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg
    46381 gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata
    46441 tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact
    46501 tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt
    46561 tgcaggcgtc cgccctcttc gataccaggg accgacaaca ggcgtgcatc cattcctggt
    46621 agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc
    46681 gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc caaatttatc ctgccaaagc
    46741 tgtttggtac tttcttgtag tttttctgcg cctgccacca catagcgtag acgatagaag
    46801 tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtacc gaacaacacg
    46861 gtgcaactgc ggtcatacac caagtccggc acaatgcggt aatgcagcgg gcttggataa
    46921 aggaacactt ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag
    46981 tgaaacagcg gtaatgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta
    47041 atctgctcga cgttcgccag aatgctttta tggctatgga cgacgccttt tggatggcct
    47101 tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg gctgctgttt tacctgcgcc
    47161 aggcgcggca tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct
    47221 tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt
    47281 ttgcctttat cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata
    47341 gcactggtca gcccttttac cccggcagtg tagttcatca ttgcgggaat gcggcgacgc
    47401 gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg
    47461 cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc
    47521 aatttgcgat aggagtctgg ggtaaagttg acgtcttcga cacatttctt cccggctccg
    47581 aagcggtaca ttgcactcag taaagattcg tacagagttt cacgcgggcg caccgccata
    47641 cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca
    47701 cgcggcgcat ccggcatcgc cacctgcgtt ggaggcaaaa tatgcagagt aatttgcggg
    47761 aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca
    47821 atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag
    47881 attttcatca gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac cactggtcgg
    47941 ccctgttcca ccagacgtac cagatgttta atagccatag gttgcgtcgg gtcgagagga
    48001 acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa
    48061 acggcaaaca ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag
    48121 acgtgattag gcgtaattag tacgcgctcg cccttcagtg cctgggtgtc acccgtaacg
    48181 cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc
    48241 tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg
    48301 acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg
    48361 gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgggatctt gattgtggtc
    48421 tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt
    48481 gtgtaaattg tcggttactg ttacagattg atgaccggca aaaaaaaacc tgcgcatctg
    48541 cgcaggctgg tgtaattcat gtgctcaaca cgaagttgac ttcacctatc aatacctctg
    48601 ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt
    48661 cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct
    48721 cacattccca cgatgaaaac acgccacccc ttgaaccaac gggcgttttc cgtaacactg
    48781 aaacaatgta agcgtttacc cactaaggta ttttcatggc gaccataaag gatgtagccc
    48841 gactggcagg cgtttcagtc gccaccgttt cccgcgtcat taataattca cccaaagcca
    48901 gcgaagcttc ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg
    48961 ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt cggtctgatc gttggtgatg
    49021 tttccgatcc gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg
    49081 gtaatttctt attgattggc aacggttacc acaacgaaca aaaagagcgt caggctattg
    49141 agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccagatg
    49201 ccgatttagc ctcattaatg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc
    49261 ctggctttga aaaccgttgt attgctcttg acgatcgtta cggtgcctgg ctggcaacgc
    49321 gtcatttaat tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt
    49381 ctgacgccga agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattccgg
    49441 ccaatgatcg gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga
    49501 ccgagctttt gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg
    49561 cggcgggcgc gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt
    49621 taattggctt tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc
    49681 gttacccaat cgtaacgatg gcgacccagg ctgccgaact ggctttggca ctggcggata
    49741 atcgccctct cccggaaatt actaatgtat ttagtccgac gctggtacgt cgtcattcag
    49801 tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc
    49861 aattccagcg ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca
    49921 ttatcaaaca gaacttctgg taacagcgga cggctattgt agttggatga cattgatgcg
    49981 ccatatgccc ctgtatcatg cagtaccaga taatcacctg ccttcacttc cggcaaggcg
    50041 cgggtttcaa catttccccc ttcctgctgg gtaaagacat cgcccgattc acataacggc
    50101 ccggcgacga cggtttccac cgttggcgcg tgttccagag aacgaccatc agctgccagg
    50161 gcactgatat ggtggtaact accgtacatt gccgggcgca tcagatcgtt gaacccggca
    50221 tcaaccagca caaagtggcg gctacccatt tgtttgacgc tccgcacctg agtaattaat
    50281 acgccagact gcgctaccag gaagcgaccc ggttcaattt ccagtttcac agggtggccc
    50341 aaatggcgag cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca
    50401 accgcctctt caccctgttg ataaggaata gaaagcccac cgcccgcaga aatagcctgt
    50461 aaatcctgac cgaattcgat aacctgacgc accatagcac cacacacctg ctccagatgg
    50521 gcataatcaa cgccagaacc aatgtgcatg tgaatgccga ctagctgcag atgatgacgt
    50581 tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg
    50641 ccaccggtat tggttttttg gctatgtccg tgaccaaacc ccggattaac gcgcagccat
    50701 acccgatgcc ctggcgaaac ctggcccagt tggtcgagcg tatcaacaga acccgcattc
    50761 accggaattt gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta
    50821 aaaacaatat catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt
    50881 tcgcctaacg agacggaatc cactttcacg ccctgctcac gcattaagcg caaaatatga
    50941 atattggaac aggctttctg tgcaaagcgc accacttcaa actgtttcag cgctgcaatc
    51001 tgccgacgaa taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa
    51061 cgcagcagat tttcggcggt gagatcggta tcggtgctga acagtgaatg tggcataaca
    51121 aactccagat gagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg
    51181 atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg
    51241 gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg
    51301 actgaggcag cacacctgct acacacctca cagccaaccg tcagccgcga actggcgcgc
    51361 tttgagaagg tgatcgggct gaaattgttt gagcgcgtac gtggacgatt acatcctacc
    51421 gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgcatt
    51481 gtcagcgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg
    51541 ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggc acgttatccc
    51601 gatgtcagct taaatatcgt gccccaggaa tcaccgctac ttgaagagtg gctctcggcc
    51661 cagcgtcatg atttaggact cactgagaca ctccatacgc ctacgggaac agaacgtacc
    51721 gaattactct ctttagatga agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa
    51781 aaggtattaa cgccggatga ttttcagggt gagaattaca tcagcctttc ccgtactgac
    51841 agctatcgcc agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc
    51901 gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac gggcgggggt aggtatttcg
    51961 gtggttaacc cgctcaccgc actagattat gcggcaagcg gtttagtggt gcggcggttc
    52021 agtattgcgg ttccgttcac cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg
    52081 ctggttcagg cgtttagtgg gcatttacaa gcggggttac cgaaactggt cacttctctt
    52141 gacgctattt tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg
    52201 ccgcggtatc aaacacaggc aaaacactgc gctcttctgg caccagtaaa ccaatttctg
    52261 tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat
    52321 aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat
    52381 taattttcgc ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca
    52441 gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc gccacacgag
    52501 tcattccggc cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag
    52561 tgcaacgtga ctcaatggca tccgccactt tatgcatcgt attggtacat aacacaatac
    52621 cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat
    52681 cccattcccc gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca
    52741 tttgcgcaga gtgaagccca ccaagccgct gtttaatgcc ttcatttatc agacgatagt
    52801 aaggaatggt ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct
    52861 cctgatgtgt gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca
    52921 ggtgcccaat ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa
    52981 tcagacgccg atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag
    53041 cgtgacattt ttggtttccg gaatgagcca gaaagtaata cccacaaacg caatgttcag
    53101 cgcagtgtag agccagaacg taccggcagc gccaatgcta tcaagcagtg tcaggaaggc
    53161 cgcgccgata atcatattcg acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc
    53221 gcggcatttc agcggctgaa tttcagagca caggatccac accactggcg cggcgctcat
    53281 cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca agccactgga
    53341 agccgtaccg ttatcaaact gcatcaggca atagcccagc accagagtgc ctaacgccat
    53401 cacgctgaaa ccaattttca gagccggttt acgacctgct ttatctaccg taaagaccgc
    53461 aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc
    53521 tgtggtcgta aagcccgcca ttttgaagat acgcggcgcg tagtacatga tgatgttcat
    53581 accggtaaac tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt
    53641 acggttgatc ttaaacagtg cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc
    53701 gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca cttcttccgc
    53761 ctcaatatga cgcccctttt ccgccagcca gcgcgggcta tttggcagga agaccaccag
    53821 aataatcagc agaactgctg gtaaagcaag aacccccaac attgcgcgcc agttaccgct
    53881 ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg
    53941 gtacatactg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg
    54001 agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc acacgagcgg cgattaacat
    54061 ctctacgctg gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc
    54121 catcaggctg tatttacgcc ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat
    54181 tgctgcgccg agcatcatgc tactaaccac ccattcctgc aaacgactgg tcagcacaaa
    54241 gtgatcggta atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa
    54301 tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat cacgcaaaga
    54361 acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt
    54421 aagacattgt gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga aacagcataa
    54481 atatggatta aattgctgcg acatgccgtt atgtgatgga tattccaatt ttaaaattaa
    54541 gttgaattat tgagattatt attaacctcc taattttaaa gcagataaaa ttcataaagt
    54601 tcattaattg ataattgata tggattattt cataaccatg atatggatta tgatgatcta
    54661 gagatataaa aaaccctgcc atgcggcagg gtcataaaag taagaggaat gaattaacgc
    54721 gccagccaac cgccatccac agcaatggta taaccattta cataatctga agcgctggag
    54781 gcaagaaaca ctaccggccc catcaggtca ctcggcagtc cccaacggcc agctggaatg
    54841 cgatcgagaa tttccgcgct acgttgttca tctgcccgca gttgttgggt attgttggtc
    54901 gccatgtaac ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcgtttgcc
    54961 atcaatcgcg tcacacccat cacgccgctt tttgatgcgg tataagaagg cacacggatc
    55021 ccgccctgga aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg
    55081 ataaagtgtt tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg
    55141 acatcgtccc agtctttttc gctgaactcg agagcgtctt cgcggcgaat caatccggcg
    55201 ttattcacca ggatatcaat atgaccaaat tcagctaccg cgcgatccag cagtccagga
    55261 atgccatcaa tctttcgcag atcggcggtc aggcttaaaa aacgacgccc cagcgccgtg
    55321 acctgctcga tggtttcagt cggttcaacg atgttaatgc caacaatgtc acagcccgct
    55381 tgcgccagcc ccaacgccat cccctggccc agcccagtat cacaaccagt gacgaccgca
    55441 actttacctt ggagagaaaa tgcacttaaa atcataacaa caccttattc gtttatgccc
    55501 acaactagcg caaatcttta acgcccacat ggtccatatc attaaagacc tggttttcac
    55561 cgaccatgcc ccagataaag gtataagctt tggttccgac gccggaatgg atcgaccagc
    55621 tcggggagat caccgcctgc tcgttatgca tcacaatgtg acgcgtttct tgcggctgcc
    55681 ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa atagaaataa acttccatcc
    55741 ggcgctcgtg ggtgtgacac ggcatggtgt tccacaagtt acctggagcc agctccgtca
    55801 gccccatact caattgacag gtttccagta catccgggac aaaatatttg ttaatcgtgc
    55861 gacggttact ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc ggtgtgactt
    55921 ttttggtggg atacgtcgta tgcgcgggtg cgcaattgta ataaaacttc gccggagtgg
    55981 cggtatcaat actggcaaag acaacttctt ttgcaccttt accaacatac agggcgtcgc
    56041 ggtgaccgat ttcatagcat tgaccatcga cagtaatcgt accggcaccg ccaatattga
    56101 taacacctaa ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta ccaacttccc
    56161 cgccaacgga aaccgtttta gttatcggca taatgccgcc aacaataatt cggtcaatgt
    56221 ggctgtaaac catggtgtac tcatcggcga caaatacctt ttcaaccaaa aattcattgc
    56281 gcagcccttg ggtatccagc gtttttgcgt gcgcaccgtg gatgctctgt cttacgtcca
    56341 catcaacctc cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc
    56401 gttttctcta taaagggatg atatgcccat gggctagggt tttcaattaa aaataaaaca
    56461 atgttttatt tttaatttaa ggattaaaaa aagtgcagtg tcgatcacga atatgtcatg
    56521 agaatggaaa aatgagggag cccagaaaat gtatgtcgac ttgccagcca tcattattca
    56581 acagattaat gacggcagat atttatcggt aaggaggaaa tgcagagcta ctcgtcacgt
    56641 tcaatggtca atgccacgcc ctggcccccg ccgatacaaa gcgttgccag ccctttgcgg
    56701 gcattacgtt tcaccatttc atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct
    56761 atcgggtgac cgagtgcgat cgcgccacca ttgacattga cccgacgctc atcccactca
    56821 agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatcg
    56881 acatcagcca actgccagcc tacacgctcc aggcaacggc gggtcgcata caccggcgca
    56941 attcccatca atgccggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg
    57001 ggtaaattca acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg
    57061 tttatggatg atgcattccc cgcggtcaca gaaccgagac tatcaaatga aggatttaaa
    57121 cgggctaaac cttctgcact ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac
    57181 gtctgaccgt tactttggat cattaccggg acgatctcat ctttaaatcg tccggcgtca
    57241 atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg
    57301 atgccatatt cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca
    57361 tcccacaacc catcatgcac cagactgtca accaactggc tattgccaag ctgttcaccg
    57421 gtgcggctat cagtcagaac atgtggtgcg cgactcatgt tttcctggcc acctgcgatg
    57481 acaatatcag cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagaccg
    57541 gaaccgcaaa cgtcattaat cgtgattgca gaaacgctgt taggaagacc acctttaata
    57601 gccgattgcc ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact
    57661 tcatccaccg catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta
    57721 ccaagttcca cggcggaatg acccgctaac gcaccacgaa agcagccgat aggtgtccgt
    57781 aacgccccga caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag
    57841 taaatgattg ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag
    57901 gaaatatgcc tgagcagtag tcagagtcat aactggcacg taaggtttgc aaccactaac
    57961 ccaccaatag atgggtagat agggcgttaa tctcccatac ttaacttggt ttatggtaaa
    58021 ttgccctcca ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc
    58081 taaagaagaa actctgtgaa gtttcgcgct ctatggcaca gccgttgatg caggcacgct
    58141 attccttcct attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt
    58201 cgcctggcct ttaacatatt ggccacataa agccttatgc cagttcatcc tctcatcaaa
    58261 aacatcagca ggtgaaggga taacgggcgc ggtaacacac tactatggca agaagattgg
    58321 taatctgatt accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt
    58381 ggcaattacc aactcactta cggaacagct ggcaaagcat atggttattg atcttcgcat
    58441 ccgtatgttg gtgagtctgg gtgttgtatt aattctgaat ctcatttttc tgatgggacg
    58501 tcatgccact attcgggtaa tgggattttt ggtattccca ttgattgcct atttcttatt
    58561 tctttccatt taccttgtcg gtagttggca acctgatcta ttaacaaccc aggtagagtt
    58621 caatcagaat acccttcacc agatatggat atcgattccc gtgatggttt tcgcctttag
    58681 ccatacgccc attatttcta cgtttgccat agacagacgt gaaaaatatg gcgaacacgc
    58741 tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca taagtgtact
    58801 gttctttgtc tttagctgcc tgctttctat tccatcttcg tatattgaag cggctaaaga
    58861 agaaggggtc accattttat cggcgctttc tatgctgccg aacgccccag catggttgtc
    58921 aatttccggg attattgtcg cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt
    58981 tggcgttatt gaaggtgcca cagaggtcgt caaaacaaca ctacagcagg ttggtgtaaa
    59041 gaaaagtcgt gcatttaacc acgcactatc aattatgttg gtatcgctga ttaccttcat
    59101 tgtttgttgc attaacccga acgcgatttc gatgatttac gcgatcagcg gcccgctcat
    59161 tgccatgata cttttcatca tgcctacgct gtcaacgtat ctcatcccgg cgcttaaacc
    59221 ctggcgttcc atcggaaatc tgattacgct gatcgtgggt atcctgtgcg tatcggtaat
    59281 gttctttagc taatatccgt tgtggatggt tcaaaattgc tacaattttg aaccattcca
    59341 tttttcataa tattcatttg ttattattcc gccctatcac ttctctcgtc atatcctaga
    59401 attatcagtt agttaaacac acaaagagat ctgaacatct taaataagac tatttaagat
    59461 gcataactta gattcgcaag atatcttctg gtcattcaaa aacaatttgc gatatagata
    59521 ttctcatacc atgagatagt tatctggacc acgttcgacc atgcaagatt aaatcagtga
    59581 aacataatca tatttaataa cgagaaatgc atttttaaat atcttaatca gaattatagc
    59641 aaatacagat taatcgcaat ttttcccagc aaaattacaa aatcaaggtg tattgacatc
    59701 atcaatcacc aatgaaatta tgcaattata tacggatagg gaggttctta acatggggct
    59761 ttgtagtcgt tataaaagtc ttacatgcaa tagttgctca atgcattgcc aaataatgcc
    59821 agaagagtca ccgcgtttgc agtactgcgc aaactcgtgt t
//
LOCUS       DAJTRH010000014        58031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTRH010000014 DAJTRH010000000
VERSION     DAJTRH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(24..278)
                     /locus_tag="OO339_000938"
     CDS             complement(24..278)
                     /locus_tag="OO339_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5925661.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO339_000939"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO339_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5925662.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO339_000940"
     CDS             1228..2694
                     /locus_tag="OO339_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5925663.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO339_000941"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO339_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5925664.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO339_000942"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO339_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5925665.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO339_000943"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO339_000943"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5925666.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO339_000944"
     CDS             5709..7265
                     /locus_tag="OO339_000944"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5925667.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO339_000945"
     CDS             7347..9161
                     /locus_tag="OO339_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5925668.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO339_000946"
     CDS             9162..10256
                     /locus_tag="OO339_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5925669.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO339_000947"
     CDS             10256..11281
                     /locus_tag="OO339_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5925670.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO339_000948"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO339_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5925671.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO339_000949"
     CDS             complement(12876..13220)
                     /locus_tag="OO339_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925672.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO339_000950"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO339_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5925673.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO339_000951"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO339_000951"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5925674.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO339_000952"
     CDS             15616..17376
                     /locus_tag="OO339_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5925675.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO339_000953"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO339_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5925676.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO339_000954"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO339_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5925677.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO339_000955"
     CDS             19113..19340
                     /locus_tag="OO339_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5925678.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO339_000956"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO339_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5925679.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO339_000957"
     tRNA            21195..21271
                     /locus_tag="OO339_000957"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO339_000958"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO339_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5925680.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO339_000959"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO339_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5925681.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO339_000960"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO339_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5925682.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO339_000961"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO339_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5925683.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO339_000962"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO339_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5925684.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO339_000963"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO339_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5925685.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO339_000964"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO339_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5925686.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO339_000965"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO339_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5925687.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO339_000966"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO339_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5925688.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO339_000967"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO339_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5925689.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO339_000968"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO339_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5925690.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO339_000969"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO339_000969"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5925691.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO339_000970"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO339_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5925692.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO339_000971"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO339_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5925693.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO339_000972"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO339_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5925694.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO339_000973"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO339_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5925695.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO339_000974"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO339_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5925696.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="OO339_000975"
     CDS             37391..37555
                     /locus_tag="OO339_000975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925697.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OO339_000976"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OO339_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5925698.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="OO339_000977"
     CDS             complement(38300..39331)
                     /locus_tag="OO339_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5925699.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="OO339_000978"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="OO339_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO339_000979"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO339_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5925700.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO339_000980"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO339_000980"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5925701.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO339_000981"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO339_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5925702.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO339_000982"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO339_000982"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5925703.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO339_000983"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO339_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5925704.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="OO339_000984"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="OO339_000984"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5925705.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="OO339_000985"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="OO339_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5925706.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO339_000986"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO339_000986"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5925707.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="OO339_000987"
     CDS             complement(53338..54114)
                     /locus_tag="OO339_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5925708.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="OO339_000988"
     CDS             complement(54119..55768)
                     /locus_tag="OO339_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5925709.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEEELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRP
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="OO339_000989"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="OO339_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56487..>58031)
                     /locus_tag="OO339_000990"
     CDS             complement(56487..>58031)
                     /locus_tag="OO339_000990"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5925710.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14196 a        15655 c        14258 g        13922 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgccatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc atgatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aataatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga tgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 ggcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct cctcctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta a
//
LOCUS       DAJTRH010000015        57975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTRH010000015 DAJTRH010000000
VERSION     DAJTRH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..1100)
                     /locus_tag="OO339_000991"
     CDS             complement(<1..1100)
                     /locus_tag="OO339_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5925711.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO339_000992"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO339_000992"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5925712.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO339_000993"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO339_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5925713.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO339_000994"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO339_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5925714.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO339_000995"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO339_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5925715.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO339_000996"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO339_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5925716.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="OO339_000997"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="OO339_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5925717.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO339_000998"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO339_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5925718.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO339_000999"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO339_000999"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5925719.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO339_001000"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO339_001000"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5925720.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKHLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO339_001001"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO339_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5925721.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO339_001002"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO339_001002"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5925722.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="OO339_001003"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="OO339_001003"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5925723.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="OO339_001004"
     CDS             11850..12014
                     /locus_tag="OO339_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925724.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="OO339_001005"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="OO339_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5925725.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="OO339_001006"
     CDS             13523..13768
                     /locus_tag="OO339_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5925726.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="OO339_001007"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="OO339_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5925727.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="OO339_001008"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="OO339_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5925728.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="OO339_001009"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="OO339_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5925729.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="OO339_001010"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="OO339_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5925730.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="OO339_001011"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="OO339_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5925731.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="OO339_001012"
     CDS             19431..19784
                     /locus_tag="OO339_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5925732.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="OO339_001013"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="OO339_001013"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5925733.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="OO339_001014"
     CDS             21084..22211
                     /locus_tag="OO339_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5925734.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="OO339_001015"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="OO339_001015"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5925735.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24394)
                     /locus_tag="OO339_001016"
                     /pseudo
     CDS             complement(24291..24394)
                     /locus_tag="OO339_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..26422
                     /gene="pdeR"
                     /locus_tag="OO339_001017"
                     /pseudo
     CDS             24448..26422
                     /gene="pdeR"
                     /locus_tag="OO339_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26570..26743
                     /locus_tag="OO339_001018"
     CDS             26570..26743
                     /locus_tag="OO339_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925736.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26833..27582
                     /locus_tag="OO339_001019"
     CDS             26833..27582
                     /locus_tag="OO339_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5925737.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27851..28069
                     /gene="osmB"
                     /locus_tag="OO339_001020"
     CDS             27851..28069
                     /gene="osmB"
                     /locus_tag="OO339_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5925738.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="OO339_001021"
     CDS             complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="OO339_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5925739.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="OO339_001022"
     CDS             complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="OO339_001022"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5925740.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="OO339_001023"
     CDS             complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="OO339_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5925741.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="OO339_001024"
     CDS             complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="OO339_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5925742.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="OO339_001025"
     CDS             complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="OO339_001025"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5925743.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32018..32608
                     /gene="ribA"
                     /locus_tag="OO339_001026"
     CDS             32018..32608
                     /gene="ribA"
                     /locus_tag="OO339_001026"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5925744.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="OO339_001027"
     CDS             complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="OO339_001027"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5925745.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35511..35606)
                     /locus_tag="OO339_001028"
     CDS             complement(35511..35606)
                     /locus_tag="OO339_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925746.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35720..35887)
                     /locus_tag="OO339_001029"
     CDS             complement(35720..35887)
                     /locus_tag="OO339_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925747.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35890..36054)
                     /locus_tag="OO339_001030"
     CDS             complement(35890..36054)
                     /locus_tag="OO339_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5925748.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="OO339_001031"
     CDS             complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="OO339_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5925749.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37533..40130)
                     /gene="topA"
                     /locus_tag="OO339_001032"
     CDS             complement(37533..40130)
                     /gene="topA"
                     /locus_tag="OO339_001032"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5925750.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40510..40761
                     /locus_tag="OO339_001033"
     CDS             40510..40761
                     /locus_tag="OO339_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5925751.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="OO339_001034"
     CDS             complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="OO339_001034"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5925752.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42066..42824
                     /locus_tag="OO339_001035"
     CDS             42066..42824
                     /locus_tag="OO339_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5925753.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42821..43411
                     /gene="cobO"
                     /locus_tag="OO339_001036"
     CDS             42821..43411
                     /gene="cobO"
                     /locus_tag="OO339_001036"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5925754.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="OO339_001037"
     CDS             complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="OO339_001037"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5925755.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44424..45044)
                     /locus_tag="OO339_001038"
     CDS             complement(44424..45044)
                     /locus_tag="OO339_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5925756.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="OO339_001039"
     CDS             complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="OO339_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5925757.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46060..46104
                     /gene="trpL"
                     /locus_tag="OO339_001040"
     CDS             46060..46104
                     /gene="trpL"
                     /locus_tag="OO339_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5925758.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46081..46174
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46196..47758
                     /gene="trpE"
                     /locus_tag="OO339_001041"
     CDS             46196..47758
                     /gene="trpE"
                     /locus_tag="OO339_001041"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5925759.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47758..49353
                     /gene="trpD"
                     /locus_tag="OO339_001042"
     CDS             47758..49353
                     /gene="trpD"
                     /locus_tag="OO339_001042"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5925760.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49354..50715
                     /gene="trpCF"
                     /locus_tag="OO339_001043"
     CDS             49354..50715
                     /gene="trpCF"
                     /locus_tag="OO339_001043"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5925761.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50727..51920
                     /gene="trpB"
                     /locus_tag="OO339_001044"
     CDS             50727..51920
                     /gene="trpB"
                     /locus_tag="OO339_001044"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5925762.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51920..52726
                     /gene="trpA"
                     /locus_tag="OO339_001045"
     CDS             51920..52726
                     /gene="trpA"
                     /locus_tag="OO339_001045"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5925763.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53106..53285
                     /locus_tag="OO339_001046"
     CDS             53106..53285
                     /locus_tag="OO339_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5925764.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53371..53871
                     /locus_tag="OO339_001047"
     CDS             53371..53871
                     /locus_tag="OO339_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5925765.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53917..54423
                     /locus_tag="OO339_001048"
     CDS             53917..54423
                     /locus_tag="OO339_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5925766.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OO339_001049"
     CDS             complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OO339_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5925767.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55361..55450
                     /locus_tag="OO339_001050"
                     /pseudo
     CDS             55361..55450
                     /locus_tag="OO339_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55478..56221
                     /locus_tag="OO339_001051"
     CDS             55478..56221
                     /locus_tag="OO339_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5925768.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56251..56790
                     /locus_tag="OO339_001052"
     CDS             56251..56790
                     /locus_tag="OO339_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5925769.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56895..57293
                     /gene="yciA"
                     /locus_tag="OO339_001053"
     CDS             56895..57293
                     /gene="yciA"
                     /locus_tag="OO339_001053"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5925770.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57333..>57975)
                     /gene="tonB"
                     /locus_tag="OO339_001054"
     CDS             complement(57333..>57975)
                     /gene="tonB"
                     /locus_tag="OO339_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5925771.1"
                     /translation="LLYTSVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVE
                     PEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESRPASPFENT
                     APARPTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALRIEGQVKVKFDVTPDG
                     RVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNILFKINGTTEIQ"
BASE COUNT        13889 a        15011 c        14508 g        14567 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag gtgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct ggcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggt aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtgtcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    26821 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    26881 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    26941 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    27001 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    27061 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
    27121 agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac tatcatcacg
    27181 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    27241 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    27301 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    27361 accggtcgcg atatgatgcg caccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    27421 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    27481 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    27541 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    27601 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga taattaagac
    27661 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
    27721 taattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    27781 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    27841 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    27901 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    27961 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    28021 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    28081 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    28141 taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    28201 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    28261 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    28321 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg taatcaggca
    28381 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccgctc catcgccttt
    28441 agggcgtact ggggccgctt tgggctcatc aatacgcccg gtttccgttg agtagaccag
    28501 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagt
    28561 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    28621 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc actcccctgc
    28681 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    28741 gcttcctgag cagaacacac cacaccatca aggtcacatt tttgcgtcag tgctgccaga
    28801 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctggcttcc
    28861 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    28921 gcctcacgcg ctgcggtcat catacgcgcc ccaccggagg catgaacatt caccatccac
    28981 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat atcgtggaat
    29041 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    29101 aacaatgtaa acatctcttt accgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    29161 acaaaggaca gcgcgtcatc acaattatga taatcaaggg caacaaccac aggagaattc
    29221 gtaacggcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    29281 gcaccgggag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgaa aataaaaaat
    29341 taacagttgc gatttttccc ctagcaggct ttttctgctc cgtcgttgcc ggagcacaat
    29401 gggacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    29461 tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca atgccagtag
    29521 agggtgtatg cggtaaaacc acatttctgg cagctataac gaggcttact acgcaccttc
    29581 tcgccaacca tgtcacgcag caccatcaga ctctctttgg cgcgcccttc ttccgcttca
    29641 tttaagtggt aatccattag cttatggaat acacgcatgg tcggatgacg ctgaagctgg
    29701 cgtgtaatat agacctgtgc agcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    29761 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    29821 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    29881 agttctctgt cctgggatat gacgcgttgc aaactttcga cggctttggc gtattctcct
    29941 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    30001 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    30061 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    30121 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    30181 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    30241 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    30301 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    30361 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    30421 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    30481 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    30541 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    30601 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    30661 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    30721 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    30781 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    30841 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    30901 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    30961 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    31021 gcgaaatggc atccacgcgc attgttgatg atttaaggcc taaattcgga aaatcaacca
    31081 gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt tcttccgcag gtggtgttaa
    31141 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    31201 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    31261 tcccgttgcc cagaccaaca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    31321 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    31381 ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct cttcagccaa
    31441 ctgttctttt actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    31501 aatatgatgt gttttttcca accagataac aaaaggtcgt ggttcctgta ctttgtcttt
    31561 gatccaggat ttaacgcctt gtccaacaag gattgccgcc gccagaatgg caaataatac
    31621 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaaca aaatcaaatg
    31681 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    31741 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    31801 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    31861 ttgataagtc ccacaatcat aactgaaaat gccagttcca agaaaaattg acagatttgt
    31921 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    31981 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    32041 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    32101 cggacacgat catgttgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    32161 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    32221 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    32281 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    32341 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    32401 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    32461 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    32521 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    32581 gaaaatgggc catttgctga acaaataacc ctcttgcatc gtgtaattca tttgcttgcc
    32641 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    32701 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    32761 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccag
    32821 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    32881 tacgcgtcac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    32941 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggtcctt
    33001 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    33061 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    33121 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    33181 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    33241 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    33301 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    33361 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    33421 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    33481 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    33541 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    33601 aaaccggctc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    33661 ttcccgccag cgcataggca accaccagcg gtggcgaggc cagccagtta gttttaacca
    33721 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    33781 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    33841 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    33901 cttttgccag ataatcagaa acgactttcg aacccggtgc caacgacgct ttgacccacg
    33961 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    34021 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    34081 gtaactgata ctgatgtccg ttcataacat aatcgactgg caggcgatct ttatgcgtgg
    34141 cattcacttt cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    34201 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    34261 acatactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    34321 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    34381 ttactgcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    34441 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    34501 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    34561 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    34621 aaaccggctg gcctaacatg gcggcttctg cttcgatccc acccacgccc caccccagca
    34681 cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag
    34741 caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat tcgaggttaa
    34801 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    34861 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    34921 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    34981 ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc gcttcgcgca
    35041 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    35101 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc agtgcgtgga
    35161 tatcctcttc ggtaactgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    35221 tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt
    35281 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    35341 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    35401 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    35461 gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga tcagtgagta
    35521 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc catccaggac
    35581 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ttttgtgttg
    35641 agataactca agctgttatt gctgcggtgt attaaaagcc ctttaattca ttaaaaatgg
    35701 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    35761 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    35821 tcgtttctgg ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac
    35881 cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc
    35941 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcac ggcggggttc
    36001 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    36061 gaatgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt tagtcaaaaa
    36121 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    36181 tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
    36241 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
    36301 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    36361 cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg cgacagccgc
    36421 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    36481 gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
    36541 agcatcaacg cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    36601 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    36661 aacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    36721 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    36781 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
    36841 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcggcatt
    36901 gcctttagag acggcatcag caatttgtgt cggcgggccc tggtgcatat gcaaagaaac
    36961 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
    37021 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac cggcaaccga
    37081 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgtcctgc
    37141 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    37201 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
    37261 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    37321 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
    37381 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    37441 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    37501 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
    37561 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    37621 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    37681 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
    37741 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaacacaccg
    37801 gcagcaccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
    37861 ggcaccggat cttctttcgg tggtgccact tcaccgttac gtaaaatctt acgtgtgttt
    37921 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    37981 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    38041 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    38101 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    38161 cgcagcgcgt tggtttcagc atcttcgcct tccagcacgt tcagcacttc gttttccggc
    38221 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    38281 aggaataccc cggtactcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    38341 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    38401 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    38461 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtaaagtc gtagttcatt
    38521 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag
    38581 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    38641 taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    38701 aaacgggctg gcggcttggt aaagtgctgg gctggtgtta gttcaacgag cgtcagagca
    38761 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    38821 acttttgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    38881 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    38941 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    39001 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    39061 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    39121 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    39181 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    39241 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    39301 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    39361 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcattctg atgagtcacc
    39421 tgcaactcca acgcttcacc agatggcgta gtcgtgctgg catcgacttc ccagaactct
    39481 tccggtacga acgctttaat ttcacgctca cgctctacca ccaggcggac cgccaccgac
    39541 tgcacacgac cggcagacag accacgagcg atctttttcc acagcagcgg cgaaaccata
    39601 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    39661 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    39721 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    39781 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    39841 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    39901 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    39961 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    40021 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cacaacccag atacttgttg
    40081 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    40141 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    40201 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    40261 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggatttttg
    40321 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    40381 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    40441 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    40501 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    40561 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    40621 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    40681 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    40741 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
    40801 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
    40861 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
    40921 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    40981 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    41041 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    41101 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
    41161 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    41221 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    41281 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    41341 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    41401 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    41461 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    41521 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
    41581 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    41641 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    41701 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    41761 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt agccaaaaac
    41821 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    41881 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    41941 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    42001 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    42061 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    42121 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    42181 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    42241 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
    42301 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
    42361 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
    42421 acgtcatgca gatcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    42481 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    42541 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    42601 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    42661 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    42721 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    42781 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    42841 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    42901 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    42961 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    43021 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    43081 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
    43141 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    43201 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    43261 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    43321 tcttgaactg gcggatacgg taagtgagct gcgccccatc agacatgcgt ttgatgccgg
    43381 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
    43441 catactttga ttaaccgtta ttacgcccgc cagaacgacg actgccgctc acctgactgt
    43501 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
    43561 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
    43621 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
    43681 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
    43741 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
    43801 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
    43861 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
    43921 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
    43981 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
    44041 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
    44101 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
    44161 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
    44221 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
    44281 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
    44341 tcgccttcac aggcgtcgaa caggagaatc aatggcgccg tatagcgcca ttttaaggcc
    44401 gcgtagtata cagagttacg cacttataag aaaggcttca catcacctac gccttcacgc
    44461 accacgaccg gcgtatcatc ggtaagatca ataaccgttg ttggtttctg cccgagatag
    44521 ccgccgtgaa taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg
    44581 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
    44641 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
    44701 ttctcctgca acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg
    44761 cccggcgtgt tgtttttcat caaacgaaac gcaacgttat ccacgaatga ataggtcgag
    44821 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
    44881 cgacaaatac gctccatggc gtttttatct tcaattttac agccgagcgc atagccggaa
    44941 tcagttggat agacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga
    45001 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
    45061 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
    45121 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
    45181 aatgatgctg acgcgcaagt gtggccagct gggtgcgttc attgggcgat tgctggcact
    45241 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
    45301 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
    45361 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
    45421 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactccacaa
    45481 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    45541 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    45601 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    45661 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    45721 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    45781 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    45841 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    45901 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    45961 atcataacgg gtctggcaaa tattctgaaa tgagctgttg acaattaatc atcgaaccag
    46021 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    46081 aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag
    46141 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    46201 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    46261 tgcgcttttt caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga
    46321 tatcgacagc aaagatgatt taaaaagcct gctgctgata gacagtgcgc tgcgcattac
    46381 agcattaggt gacaccgtca caattcaggc actttccggc aatggcgaag ccctgctggc
    46441 actactggat aacgccctgc ctgcgggtgt ggaaagtgaa caatcaccaa actgccgtgt
    46501 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc
    46561 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    46621 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    46681 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    46741 ggtgattgac catcagaaaa aaagcacccg tattcaggcc agcctgtttg ctccgaatga
    46801 agaagaaaaa caacgtctca ctgctcgcct gaacgaacta cgtcagcaac tgaccgaagc
    46861 cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga
    46921 tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt
    46981 ccaggtggtg ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    47041 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    47101 cctatttggc gcgtcgccgg aaagctcgct caagtatgat gccaccagcc gccagattga
    47161 gatctacccg attgccggaa cacgcccacg cggtcgtcgc gccgatggtt cactggacag
    47221 agatctcgac agccgtattg aactggaaat gcgtactgac cataaagagc tgtctgaaca
    47281 tctgatgctg gttgatctcg cccgtaatga tctggcacgc atttgcaccc ctggcagccg
    47341 ctacatcgcc gatctcacca aagttgaccg ttattcctat gtgatgcacc tcgtctctcg
    47401 cgtagtcggc gaactgcgtc acgatcttga cgccctgcac gcttatcgcg cctgtatgaa
    47461 tatggggacg ttaagcggtg cgccgaaagt acgcgctatg cagttaattg ccgaggcgga
    47521 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    47581 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    47641 gggtgctggt gtagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaacaa
    47701 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    47761 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    47821 cgcagcaatg gtcataacgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    47881 gaacgcctgg cgacgatgag caatccggta ctgatgcttt ctcctggccc cggtgtgccg
    47941 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    48001 ggcatttgcc tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg
    48061 ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc
    48121 ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg
    48181 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    48241 gatcgcgttt gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc
    48301 ctgctggaac aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa
    48361 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg
    48421 ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc
    48481 atgaaaattc gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa
    48541 aacgcagcgc cgttcccgcg cccggattat ctgtttgctg atatcgtcgg tactggcggt
    48601 gacggcagca acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg
    48661 ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta aatccggctc gtcggatctg
    48721 ctggcggcat tcggtattaa tcttgatatg aacgccaata aatcgcgcca ggcgctggac
    48781 gagttaggcg tatgtttcct ctttgcgccg aaatatcaca ccggattccg ccacgcgatg
    48841 ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac
    48901 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    48961 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg
    49021 gatgaagttt cattacacgc gccgacaatc gttgccgagc tgcatgacgg caaaattaag
    49081 agctatcaat tgaccgctga agattttggc ctgactccct accaccagga gcaactggca
    49141 ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    49201 gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat
    49261 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    49321 gacagagtta ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    49381 gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt
    49441 cagaatgagg ttcagccgag cacacgacat ttttatgatg cgctacaggg tgcacgcacg
    49501 gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    49561 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    49621 gatgagaaat attttcaggg gagctttgat ttcctcctca tcgtcagcca aatcgccccg
    49681 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    49741 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    49801 cttgcagccg tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag
    49861 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    49921 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    49981 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    50041 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    50101 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    50161 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgttgc gacatcaccg
    50221 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    50281 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    50341 gcggcagtgc aactgcatgg caatgaagat cagctgtata tcgatacgct gcgtgaagct
    50401 ctgccagcac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    50461 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    50521 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    50581 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    50641 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    50701 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    50761 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    50821 ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt caacgacctg ctgaaaaact
    50881 atgccgggcg tccaaccgca ctgaccaaat gccagaacat tacagccggg acgaacacca
    50941 cgctgtatct caagcgtgaa gatttgctgc acggcggcgc acataaaacc aaccaggtgc
    51001 tggggcaggc gttgctggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccgggg
    51061 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    51121 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    51181 tgggtgcgga agtgatcccg gtacatagcg gttccgccac gctgaaagat gcctgtaatg
    51241 aggcgctacg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcgg
    51301 ctggcccgca tccttatccg accattgtcc gtgagtttca gcggatgatt ggcgaagaaa
    51361 ccaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    51421 gcggcggttc gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc
    51481 tgattggtgt ggagcctggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    51541 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    51601 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggtccac
    51661 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    51721 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat catcccggcg ctggaatcct
    51781 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa cccggataaa gagcagctac
    51841 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    51901 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    51961 caaagaaggc gcattcgttc ctttcgtcac gctcggtgat ccgggcattg agcagtcatt
    52021 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    52081 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    52141 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    52201 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    52261 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    52321 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    52381 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    52441 atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    52501 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    52561 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    52621 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    52681 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    52741 gttccgctgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    52801 atatttatct ttttaccgtt tcgcttaccc cggtcgaacg tcaacttacg tcatttttcc
    52861 gcccaacagt aatataatca aacaaattaa tcccgcaaca taacaccagt aaaatcaata
    52921 attttctcta agtcacatat tcctcaggta attgttaata tatccagaat gttcctcaaa
    52981 atatatttcc ctctatcttc tcgttgcgct taatttgact aatcctcatt agtgactaat
    53041 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    53101 ataacatggc cgaacatcgt ggtagttcag gaaatttcgc cgaagaccgt gagaaggcat
    53161 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    53221 gcgcatctga agcgggtaaa aaaggcggtc aacaaagcgg tggtaataaa tcaggcaaat
    53281 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    53341 tggctcgcag tgatttcact ggagaaaaat atgaatatga agaccattga agatgtattt
    53401 attcaactgc tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    53461 ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    53521 actcatggac agattgaacg aattgatcaa gttgtggaat cagaatcaaa tctgaaaatt
    53581 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    53641 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    53701 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    53761 aaagcagcga agcttctgaa agaaaccctg gaagaagaaa aggccaccga catcaaactg
    53821 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaat
    53881 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    53941 gactggttac gtgacgccca cgcaatggaa aagcaagccg aatctatgct tgaatccatg
    54001 gccagccgta tagataatta tcctgaacta cgcgctcgta ttgaacaaca tcttagtgaa
    54061 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    54121 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    54181 ccttctgatg aaatagttaa aggctctatt ggcggatatg tcttcgagca atttgaaatc
    54241 acctgttaca cctcactatt agcagcagca aaaaatgccg gtgatacagt ctcaattccg
    54301 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    54361 ccgcaaacaa ctgagaaatt tttaattcgt tctgaaactg atggcgtaga agcgaagaaa
    54421 taataccctt tataccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    54481 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatcg
    54541 tgttgctgtg caccgcctag cttataattg gcggtggtat cgatatccat gtaccacact
    54601 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    54661 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    54721 ccattatcaa agaaggtggt gtagttaata ccggccccaa cgtaaggacg gaatttgctg
    54781 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatggtg aacggttgca
    54841 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    54901 acaccaatgt tgtcggtcgc catataagta aacgtcaagc ccagttgagt gttattggtc
    54961 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
    55021 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagaaaga
    55081 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    55141 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    55201 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
    55261 ctggttaatg tgatttaaat caatttttaa atattttctc tacgcaaata ttaactcgat
    55321 cgccacttgc cgcgctttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    55381 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    55441 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    55501 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    55561 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
    55621 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
    55681 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
    55741 agaattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    55801 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgcccaa
    55861 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    55921 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgc tggtacagga
    55981 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg
    56041 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    56101 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    56161 cctgatttct gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
    56221 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    56281 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc agcgctgatc
    56341 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
    56401 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    56461 gatgagttta ttaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
    56521 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgagtaa agaactcacg
    56581 ctgccgcaac cggtatggtc gaagctaaat ctggcctggg ctgttttctt tatcctttgc
    56641 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    56701 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    56761 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    56821 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    56881 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    56941 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    57001 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
    57061 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    57121 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
    57181 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    57241 agtatgtagc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
    57301 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
    57361 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    57421 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    57481 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
    57541 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    57601 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ccgtacttga tgtggggcgc
    57661 gcgggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    57721 tttggctgct cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
    57781 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    57841 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    57901 gcaaccatcg tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc
    57961 gaggtataga gcagc
//
LOCUS       DAJTRH010000016        54925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTRH010000016 DAJTRH010000000
VERSION     DAJTRH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..991)
                     /gene="argH"
                     /locus_tag="OO339_001055"
     CDS             complement(<1..991)
                     /gene="argH"
                     /locus_tag="OO339_001055"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5925772.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFD"
     gene            complement(1052..1828)
                     /gene="argB"
                     /locus_tag="OO339_001056"
     CDS             complement(1052..1828)
                     /gene="argB"
                     /locus_tag="OO339_001056"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5925773.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(1836..2840)
                     /gene="argC"
                     /locus_tag="OO339_001057"
     CDS             complement(1836..2840)
                     /gene="argC"
                     /locus_tag="OO339_001057"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5925774.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            2994..4145
                     /gene="argE"
                     /locus_tag="OO339_001058"
     CDS             2994..4145
                     /gene="argE"
                     /locus_tag="OO339_001058"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5925775.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4497..7148
                     /gene="ppc"
                     /locus_tag="OO339_001059"
     CDS             4497..7148
                     /gene="ppc"
                     /locus_tag="OO339_001059"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5925776.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7331..9064
                     /locus_tag="OO339_001060"
     CDS             7331..9064
                     /locus_tag="OO339_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5925777.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9213..10064
                     /locus_tag="OO339_001061"
     CDS             9213..10064
                     /locus_tag="OO339_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5925778.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10051..10392)
                     /locus_tag="OO339_001062"
     CDS             complement(10051..10392)
                     /locus_tag="OO339_001062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5925779.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10394..11272)
                     /locus_tag="OO339_001063"
     CDS             complement(10394..11272)
                     /locus_tag="OO339_001063"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5925780.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11238..13535)
                     /locus_tag="OO339_001064"
     CDS             complement(11238..13535)
                     /locus_tag="OO339_001064"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5925781.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(13586..13906)
                     /locus_tag="OO339_001065"
     CDS             complement(13586..13906)
                     /locus_tag="OO339_001065"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5925782.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(13921..15000)
                     /locus_tag="OO339_001066"
     CDS             complement(13921..15000)
                     /locus_tag="OO339_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5925783.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15309..16412
                     /locus_tag="OO339_001067"
     CDS             15309..16412
                     /locus_tag="OO339_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5925784.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16413..17810
                     /gene="ptsP"
                     /locus_tag="OO339_001068"
     CDS             16413..17810
                     /gene="ptsP"
                     /locus_tag="OO339_001068"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5925785.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            17822..18484
                     /gene="fsa"
                     /locus_tag="OO339_001069"
                     /pseudo
     CDS             17822..18484
                     /gene="fsa"
                     /locus_tag="OO339_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18495..19596
                     /gene="gldA"
                     /locus_tag="OO339_001070"
                     /pseudo
     CDS             18495..19596
                     /gene="gldA"
                     /locus_tag="OO339_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            19871..20478
                     /locus_tag="OO339_001071"
                     /pseudo
     CDS             19871..20478
                     /locus_tag="OO339_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(20505..21410)
                     /gene="yijE"
                     /locus_tag="OO339_001072"
     CDS             complement(20505..21410)
                     /gene="yijE"
                     /locus_tag="OO339_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5925786.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21504..23684)
                     /gene="katG"
                     /locus_tag="OO339_001073"
     CDS             complement(21504..23684)
                     /gene="katG"
                     /locus_tag="OO339_001073"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5925787.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24013..24903)
                     /gene="metF"
                     /locus_tag="OO339_001074"
     CDS             complement(24013..24903)
                     /gene="metF"
                     /locus_tag="OO339_001074"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5925788.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25252..27684)
                     /gene="metL"
                     /locus_tag="OO339_001075"
     CDS             complement(25252..27684)
                     /gene="metL"
                     /locus_tag="OO339_001075"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5925789.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(27687..28847)
                     /gene="metB"
                     /locus_tag="OO339_001076"
     CDS             complement(27687..28847)
                     /gene="metB"
                     /locus_tag="OO339_001076"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5925790.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29124..29441
                     /gene="metJ"
                     /locus_tag="OO339_001077"
     CDS             29124..29441
                     /gene="metJ"
                     /locus_tag="OO339_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5925791.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            29625..30233
                     /locus_tag="OO339_001078"
     CDS             29625..30233
                     /locus_tag="OO339_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5925792.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30294..30506)
                     /gene="rpmE"
                     /locus_tag="OO339_001079"
     CDS             complement(30294..30506)
                     /gene="rpmE"
                     /locus_tag="OO339_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5925793.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            30709..32907
                     /gene="priA"
                     /locus_tag="OO339_001080"
     CDS             30709..32907
                     /gene="priA"
                     /locus_tag="OO339_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5925794.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33063..34088
                     /gene="cytR"
                     /locus_tag="OO339_001081"
     CDS             33063..34088
                     /gene="cytR"
                     /locus_tag="OO339_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5925795.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34180..35139
                     /gene="ftsN"
                     /locus_tag="OO339_001082"
     CDS             34180..35139
                     /gene="ftsN"
                     /locus_tag="OO339_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5925796.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35232..35762
                     /gene="hslV"
                     /locus_tag="OO339_001083"
     CDS             35232..35762
                     /gene="hslV"
                     /locus_tag="OO339_001083"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5925797.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            35772..37103
                     /gene="hslU"
                     /locus_tag="OO339_001084"
     CDS             35772..37103
                     /gene="hslU"
                     /locus_tag="OO339_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5925798.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37170..38096
                     /gene="menA"
                     /locus_tag="OO339_001085"
     CDS             37170..38096
                     /gene="menA"
                     /locus_tag="OO339_001085"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5925799.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38189..38674
                     /gene="rraA"
                     /locus_tag="OO339_001086"
     CDS             38189..38674
                     /gene="rraA"
                     /locus_tag="OO339_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5925800.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(38759..39004)
                     /gene="zapB"
                     /locus_tag="OO339_001087"
     CDS             complement(38759..39004)
                     /gene="zapB"
                     /locus_tag="OO339_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5925801.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39430..40275
                     /gene="glpF"
                     /locus_tag="OO339_001088"
                     /pseudo
     CDS             39430..40275
                     /gene="glpF"
                     /locus_tag="OO339_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40315..41805
                     /gene="glpK"
                     /locus_tag="OO339_001089"
     CDS             40315..41805
                     /gene="glpK"
                     /locus_tag="OO339_001089"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5925802.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42035..43045
                     /gene="glpX"
                     /locus_tag="OO339_001090"
     CDS             42035..43045
                     /gene="glpX"
                     /locus_tag="OO339_001090"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5925803.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43142..43888
                     /gene="fpr"
                     /locus_tag="OO339_001091"
     CDS             43142..43888
                     /gene="fpr"
                     /locus_tag="OO339_001091"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5925804.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(43893..44321)
                     /gene="uspD"
                     /locus_tag="OO339_001092"
     CDS             complement(43893..44321)
                     /gene="uspD"
                     /locus_tag="OO339_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5925805.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44348..44647)
                     /locus_tag="OO339_001093"
     CDS             complement(44348..44647)
                     /locus_tag="OO339_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5925806.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(44859..45299)
                     /locus_tag="OO339_001094"
                     /pseudo
     CDS             complement(44859..45299)
                     /locus_tag="OO339_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45400..45999
                     /locus_tag="OO339_001095"
     CDS             45400..45999
                     /locus_tag="OO339_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5925807.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46107..46874
                     /gene="tpiA"
                     /locus_tag="OO339_001096"
     CDS             46107..46874
                     /gene="tpiA"
                     /locus_tag="OO339_001096"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5925808.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(46929..47684)
                     /gene="cdh"
                     /locus_tag="OO339_001097"
     CDS             complement(46929..47684)
                     /gene="cdh"
                     /locus_tag="OO339_001097"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5925809.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(47791..48780)
                     /gene="sbp"
                     /locus_tag="OO339_001098"
     CDS             complement(47791..48780)
                     /gene="sbp"
                     /locus_tag="OO339_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5925810.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49100..50062)
                     /gene="pfkA"
                     /locus_tag="OO339_001099"
     CDS             complement(49100..50062)
                     /gene="pfkA"
                     /locus_tag="OO339_001099"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5925811.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50243..51145)
                     /gene="fieF"
                     /locus_tag="OO339_001100"
     CDS             complement(50243..51145)
                     /gene="fieF"
                     /locus_tag="OO339_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5925812.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51293..51793)
                     /gene="cpxP"
                     /locus_tag="OO339_001101"
     CDS             complement(51293..51793)
                     /gene="cpxP"
                     /locus_tag="OO339_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5925813.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            51943..52641
                     /gene="cpxR"
                     /locus_tag="OO339_001102"
     CDS             51943..52641
                     /gene="cpxR"
                     /locus_tag="OO339_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5925814.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            52638..54011
                     /gene="cpxA"
                     /locus_tag="OO339_001103"
     CDS             52638..54011
                     /gene="cpxA"
                     /locus_tag="OO339_001103"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5925815.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54117..54791)
                     /gene="yiiM"
                     /locus_tag="OO339_001104"
     CDS             complement(54117..54791)
                     /gene="yiiM"
                     /locus_tag="OO339_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5925816.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13384 a        14861 c        14045 g        12635 t
ORIGIN      
        1 cgtcgaacag accttctttg tcttcctgca tatctttgtt gtaagccagc ggcaaacctt
       61 tcagcgtcat catcatgccg gttaacgcac cctgcacccg gccgcattta ccgcgaatca
      121 gctccagcgc atccgggttt ttcttctgcg gcattaatga tgaaccggaa gtcacgcggt
      181 ccgaaagctc cacaaacccc gcttcgccgg tgttaaagaa aatcagatct tcagcaaaac
      241 gcgacagatg caccatgccg atagcggcag cagaaagcag ttccaacaca tggtcacggt
      301 cagaaacgct gtccagactg ttacgggtcg ccgaagcaaa gcccagccag cctgctaact
      361 gttcacggtc gatttcatag gccgttcccg ccagcgcgcc acagcctaat gggctgacat
      421 ccagacgctt aagcgcatcc tgcaaacggc tttcatcacg cgccagcatc tcaacatagg
      481 ccaggcacca gtgcgcgaac gtcaccggct gggcgcgttg caggtgagtg taacctggca
      541 ttaccgcgtc ctgattgttt tgtgcggttt ccaccagcgc cgattgcagc tgccggttag
      601 ccgtcagtaa ctcgctaacg gtatctttgc accacagttt caggtcagtc gctacctgat
      661 cattacggct acgcccggta tgcagctttt tgcctaactg gcccactttg tcgatcagtt
      721 tgccttccac ccagctatgg atatcttcgg cgtcgctttc aaggatttgt tgtggcctgg
      781 cgcgaacatc ttccagcaac acgttcagcg cctcttccag ttgcgcctgc tcttctgcgg
      841 ttaacacgcc gaccgttacc aaggctttgg accaggccac agagccaaca atatcctgct
      901 ccgccagacg gtaatcaaag cgcagtgagt cgttgaattg tttgaaccgt tgatctgctg
      961 cctgggtaaa acgcccgccc caaagtgcca taactctgtt tccttatttt gaaattcaat
     1021 gccggaaagc tgcgcctccg gctaacaaaa cttaagctaa aatccgcgta cccatcggca
     1081 taccgttaaa cagtgccgga agctgctccg cataacgcca agaagcgata tctaccggac
     1141 ggcccagcgt gcgggccgca tccagcgccg cgttcacttt cactatcatg ccgtcagtaa
     1201 taatgccctg ctcaatcagt tgttctgctt tcgcggcggt catttcggca atgcgttgcc
     1261 ctttgccgtc gagaatgccg ctgacgtcgg agagcaaaat cagatccgcg cccagcgttg
     1321 ccgccagcgc cgttgccgcc tggtcggcat tgacgttcat cagttgccct tcgtctgtta
     1381 cgccaatgga gctgaccacc ggcagataac cgttctccag caaggagttg ataagcttag
     1441 gcgaacctgg ctgcgccagt ccaacatgac ctaactcttc atcaagctgg gtcactttga
     1501 cgctgtcgcc gtcaccgaga aacaaaccta cggccgcaat ctgatgtttc ttcgcccatg
     1561 ccaacagggt tttatttgcc gttcccgcca gtgctccggt gataatgtct atctgatcag
     1621 caggcgtcac ccgcaggccg tttttctttt tcaccggcag attcagccct ttcatcagct
     1681 catccaccac gcaaccgccg ccgtgcacaa tcaccagcgg acgctgatgt gactcacgat
     1741 aattcaccaa tgcgctaaac agacgttcca gcgcctcttc actatccagc agtacgccgc
     1801 ccagtttgat aattaatgga ttcatcattg cacccttaaa taagagactg cgtttccgca
     1861 tagccgaaac gaatattggc gcactgtacc gcttgtgccg ccgcgccttt cagtaagttg
     1921 tcttcggtcg ccacaataat cagatgctcg ccctgaacgg caaacccgat atcgcaaaat
     1981 ggcagcccaa cgacattttt cagcgccgga acgcctttgt catacagccg caccagcggt
     2041 ttatgggcat acgcctgctg taacgcttgc gcgacttgcg cctgggtcac acccgatttc
     2101 aggcggcagg taatggtttc gagaatgccg cgcgggaaat tgcccagatg tggggtgaag
     2161 ataacgtcag caccgaggtg tgtggcgatc tctggttgat ggcgatgagt aaagacgcca
     2221 tacggttgca ggctaacttc acaaaaacta tttgaaatcg ctgccttacg tcctgcaccg
     2281 ctcacgccgc tggtggcgtt gatcaccggc cactgattga ggtcaagaag atcggcatca
     2341 atcaacggtt tcagcgccag ctgtgccgcc gtcggataac agcctggcac cgcaatcaaa
     2401 ttcgcttctt ttaatttatt accgcaccac tccgccagac cgtaggctgc ctgttccagc
     2461 agttccgggt attgatgggt aaagccgtaa tatttttcat agaaggtggc gtcgttaaca
     2521 cgaaacgcgc cggaaaggtc gaacaccacg cagcccgctt caagaaactg cggcgctaaa
     2581 tcgtggctaa cttcatgggc ggtggcgaga aacactacgt ccacccctgg gctaaactcg
     2641 ctgatatccg acatcggctg caacggcagc tcaacgatgc cttttagctg cggatgcaaa
     2701 tcggagatta actttcccgc atcattgctt tgcgctgaaa cagtcaaagc ggttatgttc
     2761 atatgcggat ggcgatttac ataggtcact agctctgcgc cagcgtagcc gctggcaccc
     2821 acaatcagcg tattcaacat cggggctatt caccttctta tgtctggttg ccaggttaaa
     2881 cgtaaaacat tcaccttacg gctggtgggt tttattacgc tcaacgttag tgtattttta
     2941 ttcataaata ctgcatgaat attgatacta tcatgaccag aggtgtgtca acaatgaaaa
     3001 acaaattacc gccatttatc gagatttacc gcgctctgat tgccacacct tcaataagcg
     3061 ctacggaaga ggcactcgat caaagcaatg cagatttaat cactctgctg gcggactggt
     3121 ttaaagattt gggcttcaat gtggaagtgc agcctgttcc aggaactcgc aacaaattta
     3181 atatgctggc cagttacgga cagggggctg gcggcttgtt gctggcgggg cataccgata
     3241 cggtgccatt tgatgacggt cgctggacgc gcgatccgtt tacactgacg gagcatgacg
     3301 gcaagcttta cggcttaggc accgccgaca tgaaaggctt ttttgcgttt atccttgatg
     3361 cgctacgcga tgtcgacgtc acgaaactga aaaaaccgct ctacattctg gcgactgctg
     3421 atgaagaaac cagtatggcc ggagcgcgtt attttgccga aactaccgcc ctgcgcccgg
     3481 attgcgccat cattggcgaa ccgacgtcac tacaaccggt acgcgcacat aaaggtcata
     3541 tctctaacgc catccgtatt cagggccagt cggggcactc cagcgatcca gcacgcggaa
     3601 ttaacgctat cgaactaatg cacgacgcca tcgggcatat tttgcaattg cgcgataacc
     3661 tgaaagaacg ttatcactac gaagcattta ccgtgccata ccctacgctc aacctcgggc
     3721 atattcacgg tggcgacgct tctaaccgta tttgcgcttg ctgtgagttg catatggata
     3781 ttcgtccgca gcctggcatg acactcaatg aacttaatgg tttgctcaac gatgcattgg
     3841 ctccggtgag cgaacgctgg ccgggtcgtc tgacggtcga cgagctgcat ccgccgatcc
     3901 ctggctatga atgcccaccg aatcatcaac tggttgaagt ggttgagaaa ttgctcggag
     3961 caaaaaccga agtggtgaat tactgtaccg aagcgccgtt tattcaaacg ttatgcccga
     4021 cgctggtgct cggtccgggt tccatcaatc aagctcatca gcctgatgaa tatctggaga
     4081 cgcggtttat taaacccacc cgtgagttga ttatccaggt aattcaccat ttttgctggc
     4141 attaaaacgt aggccggata aggcgctcgc gccgcatccg gcaccgttgc caaactccgg
     4201 tgccgcaata atgtcggatg cgatgcttgc gcatcttatc cgacctacac ctttggtgtt
     4261 atgtatggcg attttttaac atttccataa gttacgctta tttaaagcgt cgtgaattta
     4321 atggcgtaaa ttcctgctat ttattcgttt gctgaagcga tttcgcagca tttgacgtca
     4381 ccgcttttac gtggctttat aaaagacgac gaaaagcaaa gcccgagcat attcgcgcca
     4441 atgcgacgtg aaggatacag ggctatcaaa cgataagatg gggtgtctgg ggtaatatga
     4501 acgaacaata ttccgcattg cgtagtaatg tcagtatgct cggcaaagtg ctgggagaaa
     4561 ccatcaagga tgcgttggga gaacacattc ttgaacgcgt agaaactatc cgtaagttgt
     4621 cgaaatcttc acgcgctggc aatgatgcta accgccagga gttgctcacc accttacaaa
     4681 atttgtcgaa cgacgagctg ctgcccgttg cgcgtgcgtt tagtcagttc ctgaacctgg
     4741 ccaacaccgc cgagcaatac cacagcattt cgccgaaagg cgaagctgcc agcaacccgg
     4801 aagtgatcgc ccgcaccctg cgtaaactga aaaaccagcc ggaactgagc gaagacacca
     4861 tcaaaaaagc agtggaatcg ctgtcgctgg aactggtcct cacggctcac ccaaccgaaa
     4921 ttacccgtcg tacactgatc cacaaaatgg tggaagtgaa cgcctgttta aaacagctcg
     4981 ataacaaaga tatcgctgac tacgaacgca accagctgat gcgtcgcctg cgccagttga
     5041 tcgcccagtc atggcatacc gatgaaatcc gtaagctgcg tccaagcccg gtagatgaag
     5101 ccaaatgggg ctttgccgta gtagaaaaca gcctgtggca aggcgtaccg aattacctgc
     5161 gcgaactgaa cgaacaactg gaagagaatc tcggctacaa actgcccgtc gaatttgttc
     5221 cggtacgttt tacctcgtgg atgggcggtg accgcgacgg caacccgaac gtcactgccg
     5281 atatcacccg ccacgtcctg ctgctcagcc gctggaaagc caccgatctg ttcctgaaag
     5341 atattcaggt tctggtttct gaactgtcga tggttgaagc gacccctgaa ctgctggcgc
     5401 tggttggcga agaaggtgcc gcagaaccgt atcgctatct gatgaaaaac ctgcgttctc
     5461 gcctgatggc gacacaggca tggctggaag cgcgcctgaa aggcgaagaa ctgccaaaac
     5521 cagaagggct gctgacacaa aacgaagaac tgtgggaacc gctctacgct tgctaccagt
     5581 cacttcaggc gtgtggcatg ggtattatcg ccaacggcga tctgctcgac accctgcgcc
     5641 gcgtgaaatg tttcggcgta ccgctggtcc gtattgatat ccgtcaggag agcacccgcc
     5701 ataccgaagc gctgggcgag ctgacccgct acctcggtat cggcgactac gaaagctggt
     5761 cagaggccga caaacaggcg ttcctgatcc gcgaactgaa ctccaaacgt ccgcttctgc
     5821 cgcgcaactg gcaaccaagc gccgaaacgc gcgaagtgct cgatacctgc caggtgattg
     5881 ccgaagcgcc gcaaggctcc attgccgcct acgtgatctc gatggcgaaa acgccgtccg
     5941 acgtactggc tgtccacctg ctgctgaaag aagcgggtat cgggtttgcg atgccggttg
     6001 ctccgctgtt tgaaaccctc gatgacctga acaacgccaa cgatgtcatg acccagctgc
     6061 tcaatatcga ctggtatcgc ggcctgattc agggcaaaca gatggtgatg attggctatt
     6121 ccgactcagc aaaagatgcg ggcgtgatgg cagcttcctg ggcgcaatat caggcacagg
     6181 atgcattaat caaaacctgc gaaaaagcgg gtattgagct gacgttgttc cacggtcgcg
     6241 gcggttccat tggtcgcggc ggcgcacctg cccatgcggc gctgctgtca caaccgccag
     6301 gaagcctgaa aggtggcctg cgcgtgactg aacagggcga gatgatccgc tttaaatatg
     6361 gtctgccaga aatcaccgtc agcagcctgt cgctttacac cggggcgatt ctggaagcca
     6421 acctgctgcc accacctgag ccgaaagaga gctggcgtcg cattatggat gaactgtcag
     6481 tcatctcctg cgatctctac cgtggctacg tacgtgaaaa caaagatttt gtgccttact
     6541 tccgctccgc tacgccggaa caagaattgg gcaaactgcc gttgggttca cgtccggcga
     6601 aacgtcgccc aaccggcggc gtcgagtcgc tgcgcgccat accgtggatt tttgcctgga
     6661 cgcaaaaccg cctgatgctc cccgcctggc tgggtgcagg tacggcgctg caaaaagtgg
     6721 tcgaagatgg caaacagagc gagctggaag ccatgtgccg cgattggcca ttcttctcga
     6781 cgcgtctcgg catgctggag atggtcttcg ccaaagcaga cctgtggttg gcggaatact
     6841 atgatcaacg tctggtagac aaagcactgt ggccgttagg taaagagtta cgcaacctgc
     6901 aagaagaaga catcaaagtg gtgctggcga ttgccaacga ttcccatctg atggccgatc
     6961 tgccgtggat tgcagagtct attcagctac ggaatattta caccgacccg ctgaacgtat
     7021 tgcaggccga gttgctgcac cgctcccgcc aggcagaaaa agaaggccag gaaccggatc
     7081 ctcgcgtcga acaagcgtta atggtcacta ttgccgggat tgcggcaggt atgcgtaata
     7141 ccggctaatc ttcctcttct gcaaaccctc gtgcttttgc gcgagggttt tctgaaatac
     7201 ttctgttcta acaccctcgt tttcaatata tttctgtctg cattttattc aaattctgaa
     7261 tataccttca gatatcctta aggaattgtc gttatattcg gcgatatttt tttcaagaca
     7321 ggttcttact atgcattcca cagaagtcca ggctaaaccc ctttctagct ggaaagccct
     7381 gggttgggca ctgctctact tttggttttt ctctactctg ctacaggcca ttatttacat
     7441 cagtggttat agtggcacca acggcattcg cgactcgctg ttattcagct cgctgtggtt
     7501 gatcccggta ttcctctttc cgaagcggat taaaattatt gccgcagtga tcggcgtggt
     7561 gctatggacg gcctctctgg cggcgctgtg ctactacgtc atctacgggc aggagttctc
     7621 gcagagcgtt ctgtttgtga tgttcgaaac caacaccaac gaagccagcg agtatttaag
     7681 ccagtatttc agcctgaaaa ttgtgcttat cgcgctggcc tatacggcgg tggcagttct
     7741 gctgtggaca cgcctgcgcc cggtctatat tccaaagccg tggcgttatg ttgtctcttt
     7801 tgccctgctt tatggcttga ttctgcatcc gatcgccatg aatacgttta tcaaaaacaa
     7861 gccgtttgag aaaacgttgg ataacctagc ctcgcgtatg gagcctgccg caccgtggca
     7921 attcctgacc ggctattatc agtatcgtca gcaactaaac tcgctaacaa agttactgaa
     7981 tgaaaataat gccttgccgc cactggctaa tttcaaagat gaatcgggta acgaaccgcg
     8041 cactttagtg ctggtgattg gcgagtcgac ccagcgcgga cgcatgagtc tgtacggtta
     8101 tccgcgtgaa accacgccgg agctggatgc gctgcataaa accgatccga atctgaccgt
     8161 gtttaataac gtagttacgt ctcgtccgta caccattgaa atcctgcaac aggcgctgac
     8221 ctttgccaat gaaaagaacc cggatctgta tctgacgcag ccgtcgctga tgaacatgat
     8281 gaaacaggcg ggttataaaa ccttctggat caccaaccag cagacgatga ccgcccgcaa
     8341 taccatgctg acggtatttt cgcgccagac cgacaagcag tactacatga accagcaacg
     8401 tacgcagagt gcgcgtgaat acgacaccaa cgtgctgaag ccgttccagg atgtgctgaa
     8461 tgaccctgcg ccgaagaaac tgatcatcgt tcatctgctg ggtacgcata tcaaatacaa
     8521 ataccgctac ccggaaaatc agggcaagtt tgatggcaat accgatcatg ttccgccagg
     8581 attaagcgca gaagagctgg aatcatataa cgattatgac aacgctaacc tgtataacga
     8641 tcatgtggtt gccagcctga ttaaagactt taaagcggca gacccgaacg gattcctggt
     8701 ttacttctct gaccacggtg aagaggttta cgacacgccg ccgcacaaaa ctcaggggcg
     8761 taacgaagac aacccgacgc gccacatgta caccattccg ttcctgctgt ggacgtcgga
     8821 aaaatggcaa gcgactcatc cccgtgattt ctcacaggat gtcgatcgta aatacagcct
     8881 ggcggaactg atccacacct ggtcagattt ggcgggctta tcttacgacg gttacgatcc
     8941 aacccgttca gtggtgaatc cgcagttcaa agaaactacc cgctggattg gtaacccgta
     9001 caagaaaaac gcgctgatcg attacgacac tctgccgtat ggcgatcagg tgggtaatca
     9061 gtagatgacc tttgtatcgc gtctggcgct atttgccgga tgcgatactg ccgcaatgtt
     9121 tacatcgaac tcccctcagg ccacactttt caggtggcct tttttaataa tgccagttac
     9181 tgttttcccg tctacatacc taagggaata acatgtatca cgacgtcagc tatctgctct
     9241 cccgcctgat caacggcccg ctgtccctgc gccagattta ctttgccagc agtaacggcc
     9301 cggtgcccga tcttgcgtat caggtcgatt ttccacggct ggaaattgtg ctggaaggtg
     9361 agtttgttga taccggcgct ggagcaacgt tagttcccgg cgatgtgctg tacgttgccg
     9421 ctggtggctg gaatttccca caatggaaaa cacccgctac cacctttagc gtgctgtttg
     9481 gcaaacagca actcggcttc agcgtcgtgc aatgggatgg caaacaatat caaaatctgg
     9541 cgaagcaaca cgtcgcccga cgcggcccac gcataggttc ttttctgcta caaacgctca
     9601 atgaaatgca aatgcagccg caggagcagc aaacggcaag gcttatcgta gccagcctgc
     9661 ttagccactg ccgcgatttg ctcggcagcc aaatccagac cgcctcacgc agccaggcac
     9721 tatttgaagc tattcgcgat tatatcgacg aacgctatgc ctccgcgctt acccgcgaat
     9781 ctgtcgcaca ggcattctat atttcaccaa actatctctc gcacctgttt caaaaaacgg
     9841 gggccattgg ttttaacgaa tacctgaatc acacgcgact ggagcacgct aagacgttat
     9901 tgaaaggcta tgacctgaag gtaaaagaag tggcgcacgc ctgcggcttt gtcgacagca
     9961 actacttctg ccgtctgttt cgtaaaaaca ccgaacactc gccgtcggaa tatcgccggc
    10021 agtaccacag ccaactgaca gaaaaaccga ctactccaga ataagatggg tttgctgcgg
    10081 cgcagaaagc actttgcgca ccgcgctcat tacccgctgc ggctcacgca ggaatgcgta
    10141 gatgctgcat tgcacatagc ggcaatgttc aaatcgctcg gcaccggcaa gctcgatatc
    10201 cgtaatcaac agagcaacat ccgcccgacg gatatcctcg tccgctaaac gattctccgt
    10261 tcccagcgcg ccctgagttt caatgctgac gccccacttc tctaactggc acaacttttc
    10321 cagccgttcc gccgccatat aagtatgcgc cacgccgctt acgcaggcgg ttactgccac
    10381 caggtatgcc attttaacct cccacggtaa cctgaaatcc ggcccgttct gccatttcgc
    10441 gcatcgttgt cacatcggct gacgacggcg caggcacctc tttcatcgac catgttttcc
    10501 ccagcaggcg gtatttcggt tcgccgtact gatgaaacgg taacagatgg atctgcctga
    10561 ttttcagcgg gatcagtaca tccagcgcct gctgcatatt ctcccggctg agcgtgaaac
    10621 cagggatcaa cggtaaacgc gggatcacgt tgacaccctc actcaccagc aaacgcagat
    10681 tctccagcac gcgtggcagg ttcatcttca ccacatcccg cgcctgagtc gcgtccataa
    10741 tttttaaatc gaacagcact tcatcgcaca atttcgccag cggtaacagc ttgcttgctg
    10801 gcgcgtctcc ggcagtttca atggcgcatg agacacccca cagccgcagt cgctgtaaaa
    10861 aacgggtagc aaactccgcc tgcattaaca cttcgccgcc agaaagcgtg acgccgccgc
    10921 cggacgtgcg aaagaaaatg tcatctttca tcacttcccg ttccagagcg tcaaggctga
    10981 tatcgcgacc aatccgttca aacgccccgg aggggcattc atccgcatca cgcaaacatt
    11041 tcgcacagtg cagacatttc gcctctctgc gtaccgtctg gattttgccg gagatcgact
    11101 ccggattagc acaccacggg caaagatgcg gacagccttt aaaaaagacc accgtacgaa
    11161 tgccctcgcc gtcattcagt gaataacgct gaatgttgaa aatgcgcgcc acatcatcgc
    11221 ggcgcgtttc cacaacgtta cagctgatgc gctgtccggc ggatgatgtc atcctggatc
    11281 tccttcgaca gttcgacaaa gaaggcgctg tatccggcaa cgcgcaccac cagcccggca
    11341 taatcttgtg ggcgctgttg cgcttcccgc aacgtgtcgg cgttcaccac gttaaactga
    11401 atatgctgca acttaagctg ggtaaacacc cgtaagaagt cggccagttt gcgtaatccc
    11461 gcttcacctt ccagggtcgc cggagtgaat ttcacgttca gcaacgtacc gttagacagc
    11521 agcgtattat cgagcttact gactgacttc agtaccgccg ttggcccttg tgcgtcctga
    11581 cccagcatag gtgacaagcc gccgtctgcc agttgttccc cggcaaaacg accgtctggc
    11641 gtcgcgccaa ccaccgatcc caacggaacg tgagcagaaa cggtatacga tcccggcgtg
    11701 aagtaaccgc cgcgcgggtt ctggtatttt tccacttctt tgcagtagtg gcgcaacagt
    11761 tcggcgctaa tgttatccac ctcgtcgata tcgttaccgt atttctcaaa gcggttaatt
    11821 aagcgagcgc ggactttttc gccttctggc gttgcaaagt tggcttttaa taccgacagc
    11881 aattcgtcaa aacttaaacg ctgttgctca aaaaccatcc ctttcaacgc atggagagaa
    11941 tcgctcaggt tggcgatacc aattccctgt acgccggaga agttataacg cgcgccgcca
    12001 tcggtaatat cgcgaccttt ttccagacaa tcgctgataa aagacgagag cagcggtaca
    12061 ggtgcccagt cgcgatggcc gatatcacaa atattgctgc cctcaaccat cagggtgatg
    12121 tagtggctga tcttggcgcg gatctgctcc agtaaacctt cataagtcag cgcagcattg
    12181 ccttcatttt catgcaggca gatttccatc actttcagca gattaaacat cgcgatgtca
    12241 tgcaagccgt aggttctgcc gggaatagat aattccacac agcccactac ggaatagtcg
    12301 cgcgcatctt ccagcgaaac gccacggttg aggaacgctg gcaccaccac ttcatcgtta
    12361 aagatttgcg gaataccggt gccgaggcga atggtttcgg cggttttcat caggaacggc
    12421 gtgtcgatca aggcgttagt gcgcacgccg aggttcggtt gcggtaattg cacgctttga
    12481 taggcgtcaa ggcaaaggaa cgaaagcacg ttcaccgcgc tacgtccgtt ctcggttaac
    12541 ccgccgagca gtgcggtata gccggtcggg aaaccggcga aataacgcgc gctactggtg
    12601 gagcgcaaca gcacgatgtc gttgcatttc acccataaag attcgagcag ttctttcagg
    12661 aacgccggat cttcgccctg ggttaaagat gcctgataga acggcaacat atactggtcg
    12721 aagcgcccta aagacagcga actggcgttg gattcgtatt gcagaatgat gttcatgtac
    12781 cagaataact ggcacgcctg ccagaacgtc tgcggcttat gttgcgcgtt atggcgggag
    12841 atctccgcaa tagtcagcag ctcttcgcga cgctgggcat ctgtgcagtt tgccgccatc
    12901 gtttccgcca gttcggcgta acgcaaaatg tgtttctgcg aggcttccag cagtaacaac
    12961 gcggcctgat aaaagtgatt ctccggctgt tgctgacaat gttgctgcat ctgtgccacc
    13021 agctcaccca gaccgtgatt caacaggcgt gggtaatcaa taataatgtg cccctggcct
    13081 ttatccgtct ggttgatgct gaaaatctgc gtgttggtcg cggcttttac ttcatctgtc
    13141 atctgcccgt tgatgaaatc tttcatcgaa cgtttttccc agtacgggaa caactcttca
    13201 cggtagatac gtttatcttc ttcgctgata gcaaagcggt cctgcggacg cgtcgggaat
    13261 tgatccagct ctttcagcag ccagtaaggg tccatttccg gcgacataat cccggcgcgc
    13321 ggttttacgg tgcggttacc ggcaatcagt tcttcatcac gaatcgaaat ttcaacatgt
    13381 tcaaggatat acgctgttgc tttcgcccgg cgcaatatca ccggttcgcc ttcggtttgc
    13441 cgatggctgg cggtataaag cagcgcccgc tccagcgaga tttcacgggt attggcaaac
    13501 agtgcagttt tgaggcgaga gatacgattc gtcatgagga actcctgtgg gaaagcgaac
    13561 ccgcccttgc gagcgggaga agaacttaag cggtttgcgc caggtgagct tcaattttgc
    13621 tcataatggc gtcagcacgt tttactgcat cgctgatgtt gacgcgcacg atggtcttgc
    13681 cagcaaaacg ctcttcaaac ttgatgccga tatctttggt cagaatcacc atatcagcgc
    13741 tcgccacgtc ttccgcagtc agttcatttt ccagaccaat tgacccctgg gtttctactt
    13801 tcacttccca gcctttcgct ttcgcggcac tttccagcgc ctctgctgcc atataggtat
    13861 gggcaacacc tgaaggacat gcggttactg caataatttt cgtcatggct cgttcctcaa
    13921 ttaattaatt tcaaaatcca aatccaggtc gtcttctttt tcatcagtcg aagacccatt
    13981 tttacgcgcc agacttttca gcacgttcac acaaacagcc gtcaccactg ctccgacagc
    14041 cactgctgcg atataaccca acttaccttc aaccaccggc agcacaatca gaccacccca
    14101 gcctgcatag cactgcgcac cgaccagcgc cgcagttact gcaccacaaa ctgaaccgac
    14161 cataatggaa ggaatgacac gcagcggatc ggcagcggcg aaaggaatcg ccccttcagt
    14221 aacgccaaca cagcccatca ccagtgccgc tttgccggtt tcgcgctctt ctgcggagaa
    14281 atttttacga ccaatcagcg tcgccagccc catccccagc ggcgggatac aaatacccac
    14341 ggcagcaatg gccaccacgg tataaacacc ctgagcaacg caaatcagca tgaaggcata
    14401 ggccactttg ttaaccggac cgcccatatc gaacgccagc atcagcccca tgatcaccgc
    14461 cagcataaca atgctgccct gctgcatccc ctgaagccac tgagtcaggc tgttggtcaa
    14521 cgcccctaca ggctcgccca agccccacat catgatgcct gcggtaatca gtgtgccgat
    14581 gataggaata atgaagatgg gcatcaccga gcgcagaact ttatgcaccg gaattttctt
    14641 caggtaatgc accacgatgc cgccgataat cccggcgatc agcgcaccaa agaagcccgc
    14701 accaaagctg ttaccaaccc aggcaccgat agcgcacgga gccagcgcag aacgctctgc
    14761 aatggagtaa ccaatgtaag cggcgaggaa aggcaccatc agcgtcaaac ctgcaacgcc
    14821 gatatcaaac agttttttca aattcggatc ggctacggca tccggcactg cgcctttgcc
    14881 atacaacatg acggaaaccg ccagcaaaat accgcccgat accacgaagg gaatcatgtg
    14941 tgaaacgccc gtcattaaat gctgacgggt atttttcagg atctgcacca actcattcat
    15001 aagctgctcc cgggcttgca aaagcccatg tccatatttt tgatggagga aacaataggc
    15061 aatcgcaggc ggagcaaaca gtggataaaa aggccattta ctggactttt gtaatatcag
    15121 tacaaaaatg cgatccgcct cataacttgc gataatccgg aagcatgccg caaaacggcg
    15181 tcttgtgaga cgcgtcgcaa attgacggtt acattacatt tgtccagctt ttggcagatt
    15241 tgtcacatgg caggccgtta ccgtgcgctc ttatcctgta aacagaaaac cgtaccggga
    15301 gaaaggcaat ggccctgatt gtggaattta tttgtgagct acctaacggc gtacatgcgc
    15361 gtccggcaag ccacgttgaa acgctgtgta atactttttc atctcaaatt gagtggcata
    15421 acctgcgtac tgaccgtaaa ggcaacgcca aaagcgctct ggcgctgatt ggcaccgata
    15481 cgctggtggg tgataactgc cagctactga tttccggggc cgacgaacag gaagggcacc
    15541 agcgtttaag ccaatggctg cgcgatgaat tcccccactg cgatgcgccg ctggcggaag
    15601 ttaaatctga cgaactggaa ccactgccgg tttcactgac caatctgaat ccgcaaatta
    15661 tccgcgcccg caccgtgtgc agcggtagtg caggcggcat tctgacgccg atctcttctt
    15721 tagatctcaa tgcgctgagt aatcttcccg cagccaaaga cgttgacgcc gagcaatccg
    15781 cactggaaaa cggcctgacg ctggtactga aaaacattga gtttcgtctg ctggatagcg
    15841 acggtgctac cagcgcgatt ctggaagcgc accgatccct ggctggcgat acttccctgc
    15901 acgaacattt actggcaggc gtcagcgccg gattaagctg cgccgaagcg attgtcgcca
    15961 gtgcgaatca cttttgcgaa gagtttgccc gttccagcag cagatacctg caagaacgtg
    16021 ccctggacgt acgcgacgtc tgcttccagt tactccagca aatctacggt gagcaacgct
    16081 tcccggcacc gggcaaactg acgcagtccg ccatttgtat ggctgatgaa ctgaccccca
    16141 gccagttcct cgaactggat aaaaatcacc tcaaaggatt gttactgaaa agcggcggca
    16201 ccacctcaca tacggtgatc cttgcccgtt cgttcaacat tccgacgctg gttggtgtgg
    16261 atattgatgc cctcactccg tggcagcatc aaacgattta tatcgacggc aacgccgggg
    16321 cgattgtggt tgagccagga gaagccgtag ctcgttatta tcagcaagaa gcccgcgtac
    16381 aggacgccct gcgtgagcaa cagcgtgtct agctgaccca acaagcccgt accgctgacg
    16441 gtatccgcat tgaaattgcc gctaacatcg ctcactccgt ggaagcgcag gccgcattcg
    16501 gcaatggtgc ggaaggcgtt ggtttgttcc gcactgaaat gctctatatg gatcgcacca
    16561 gtgcaccggg cgaaagcgag ctgtacaaca ttttttgtca ggcgctggag tctgccaacg
    16621 gacgcagcat tattgtgcgc actatggaca ttggcggcga caaacccgtt gattatctaa
    16681 acattcccgc agaggcaaac ccgttcctcg gttatcgcgc cgtgcgtatt tatgaagaat
    16741 acgcgtcgtt atttaccaca caactacgct cgatcctgcg cgcctccgct cacggcagcc
    16801 tgaaaatcat gatcccgatg atctcctcaa tggaagagat cttatgggtg aaagaaaaac
    16861 tggcagaagc caaacagcaa ctacgtaacg aacacattcc gtttgatgag aagatccagc
    16921 tcggcattat gctggaagtg ccgtcggtga tgttcatcat cgatcaatgc tgcgaagaga
    16981 ttgatttctt tagtattggt agtaatgacc tgacgcaata tctgctggca gtggatcgcg
    17041 ataacgctaa ggttactcgt cactacaaca gcctgaatcc ggcattcttg cgggcgctcg
    17101 attacgacgt gcaggcggtg catcgccagg gtaaatggat tggtctgtgc ggtgagctgg
    17161 gagcgaaagg ttccgtgctg ccgttgctgg tcggcttagg gctggatgaa ctcagcatga
    17221 gcgcaccatc aattccggcg gcgaaagcgc ggatggcgca acttgatagc cgtgagtgcc
    17281 gcaagttgct caaccaggca atggcctgcc gtacttcgct ggaagtggaa cacctgctgg
    17341 cgcaattccg catgacccaa caggacgcac cgctggtcac cgccgagtgc atcacgctgg
    17401 aaagtgactg gcgcagcaaa gaagaagtgc tcaaaggcat gaccgataac ctgctgctgg
    17461 cgggccgctg ccgctatccg cgtaaactgg aagccgactt atgggcgcgc gaggccgttt
    17521 tctctaccgg tctgggcttt agttttgcca ttccacacag caaatcagaa cacattgagc
    17581 aatccaccat cagcgtggcg cgtctgcaag cgccggtgcg ctggggcgat gatgaagcgc
    17641 aattcatcat tatgttaacc ctgaacaaac acgctgcggg cgatcagcac atgcgcattt
    17701 tctcgcgcct cgctcgccgc atcatgcacg aagaattccg taacgcgctg gttaacgccg
    17761 cctctgccga cgctatcgcc agcctgctgc aacatgaact ggaactgtaa aaggaaacat
    17821 catggaactg tatctggaca ccgctaacgt cgcagaagtc gaacgtctgg cacgcatatt
    17881 cccgattgcc ggggtgacaa ctaacccgag cattatcgct gccagcaagg agtccatctg
    17941 ggaagtgctg ccgcgtctgc aaaaagcgat tggtgatgag ggcattctgt ttgctcagac
    18001 catgagccgc gacgcgcagg ggatggtgga agaagcgaag cgcctgcgcg acgctattcc
    18061 gggtattgtg gtgaaaatcc cggtgacttc cgaaggtctg gcagcaatta aaatactgaa
    18121 aaaatagggt attactacac ttggcaccgc tgtatatagc gccgcagaag ggttattagc
    18181 cgcactggca ggggcaaaat acgttgctcc gtatgttaac cgcgtagatg cccagggcgg
    18241 ggacggcatt cgtacggttc aggagctgca aacgctgtta gaaatgcacg cgccagaaag
    18301 catggtgctg gcagccagct ttaaaacgcc gcgtcaggcg ctggactgtt tactggcagg
    18361 atgtgaatcc atcaccctgc ccttagatgt agcgcaacaa atgctcaaca cccctgcggt
    18421 agagtcagct atagagaagt tcgaacacga ctggaatgcc gcatttggca ctactcatct
    18481 ctaaaggagc aattatggac cgcattattc aatcaccggg taaatacatc cagggcgctg
    18541 atgtgattaa tcgtctgggc gaatacctga agccgctggc agaacgctgg ttagtggtgg
    18601 gtgacaaatt tgttttagtt tttgctcaat ccactgtcga gaaaagcttt aaagatgctg
    18661 gactggtagt agaaattacg ccgtttggcg gtgaatgttc gcaaaatgag atcgaccgtc
    18721 tgcgtggcat cgcggagact gcgcagtgtg gcgcaattct cggtatcggt ggcggaaaaa
    18781 ccctcgatac tgccaaagca ctggcacatt tcatgggtgt tccggtagcg atcgcaccga
    18841 ctatcgcctc gaccgatgca ccgtgcagcg cattgtctgt tatctacacc gatgagggtg
    18901 agtttgaccg ctatctgctg ttgccaaata acccgaatat ggtcattgtc gacaccaaaa
    18961 tcgtcgcggg cgcacctgca cgtctgttag cagcgggtat cggcgatgcg ctggcaacct
    19021 ggtttgaagc gcgtgcttgc tctcgtagcg gcgcgaccac catggcgggc ggcaagtgca
    19081 cccaggcggc gctggcactg gctgaactgt gctacaacac cctgctggaa gaaggcgaaa
    19141 aagcgatgct tgccgccgaa cagcatgtag taaccccggc gctggagcgc gtgattgaag
    19201 cgaacaccta tttgagcggc gttggttttg aaagcggcgg ctggcggcgg ctcacgcggt
    19261 gcataacggc ctgaccgcta tcccggacgc gcatcactat tatcacggtg aaaagtggca
    19321 tttggtacgc tgacgcagct ggtactggaa aacgcgccgg tggaggaaat cgaaaccgta
    19381 gctgcactta gccatgcggt aggtttgcca ataactctcg ctcaactgga tattaaagaa
    19441 gatgtcccgg cgaaaatgcg cattgttgca aaagcatctt gtgcagaagg tgaaaccatc
    19501 cacaacatgc ctggcggcgc gacgccagat caggtttacg ccgcactgct ggtagctgac
    19561 cagtacggtc agcgtttcct gcaagagtgg gaataaccta ctccaaactc ccggcttgtc
    19621 cgggagtttg aacgcaaaat tgcctgatgc gctacgctta tcaggcctac gcaatctctg
    19681 caatatattg aatttgcgtg cttttgtagg ccggataagg cgttcactcc gcatccggca
    19741 ctctgtgcca gtaatctgaa actcccacgt tgttcaggag ttttttatcc gcttctggac
    19801 attttctcca cagaacaatc gttagcccgg aaggtcgaat ctgtgactct tattttcacg
    19861 gacgaaccag atgaaagctt cactggcgct gctcagcctg ctaacggcat ttaccagcca
    19921 ctccttaaaa tctcccgccg ttccaccaac cgtggtacaa atccaggcca ataccaacct
    19981 tgccatcgca gacggtgcca gacagcaaat tggcagcacg ctattttacg atcgcagctt
    20041 acctatcctg gcggtgatgt tccgcaagaa cgcggtgtat gttccgatat ggtgatccgc
    20101 gcattgcgca gccagaaggt cgatttgcag aaactggttc acgaagatat ggcgaagaat
    20161 tttgctgagt acccgcaaaa atggaagtta aagcgcccgg acagcaacat cgatcaccgt
    20221 cgggtgccta atctggaaac ctggtttagc cgccacaaca aaacacgccc catcagtaaa
    20281 aatgccggtg actatcaagc gggcgatatt gtctcctggc gattagataa cgggctggcg
    20341 catattgggg tggcgtcaga tggcttcgcc cgcgacggta cgccgctggt gatacataac
    20401 attggcgcag gcgcgcagga agaagatgtg ctgtttagct ggcggatggt ggggcattat
    20461 cgttattttg ttaaataaga gatcgcccca catgaagaaa aaattcagat gctttttaca
    20521 ctgacagctt cttttttctt acggctcacc agcgccagtg ccagcacaat cagcacaata
    20581 ccgctacctt caacggcccc cggattctca ccgagcagcc accaggaaaa gagtacgcca
    20641 caaacgggaa cggccagtgt gcttaagctg gcaatactgg caggcaagtt tttcagcaca
    20701 aacagccata agctccacgc tagcgccgtc gccagaatcg cactgtaggc cagcgcccag
    20761 aacacggtgg gctgccagtc aatttcacgt tgcggcacca gtaaagcgac cacactcatc
    20821 accagcgccg catacagcat ctgccaggat gttaacgata ataaatccac gcgcggatga
    20881 cgggcgtata ggcgtttagc aacaatcgcg ctcgcccccc aactaacacc ggagaggatt
    20941 gccagcatgg cacttttcat cgaagagaaa tcgagttgcc acggctgcaa caccaaaaat
    21001 aagccgaaag cggcaatcag aatcgcgaaa tattgcccac gtcgcaggcg ttcaccgaga
    21061 aacaacgcgg cgaaaatcac cacccagaac ggcatggtat agctcaggat cgccaccttc
    21121 cccgcacctc cgctgaccaa cgcccactgc gccagaccaa ccatcccgca ggtttgtaac
    21181 agggcaatgg ctaaggtgta tttaaacggc gtcgggcgca ttccgcgacc acgtaataaa
    21241 aggacgatga ataaaacgag agcgccgaaa atgcagcgta aggcggtaaa gtcgaaggca
    21301 ccgatgtaac tggtgacttg cttcatgaaa atccagctat aactccagat aagtgtgagc
    21361 acaaccaagc cactgattgc cagtgggttg ctctttcctg cggcagacat gagagatcca
    21421 gtgtgtagtt ttcctgtgga cactatacgc cggtaaagaa acttcagcga ctgcctgaaa
    21481 gacagtcgct gaagaaggtc agattacagc aggtcgaaac ggtcgaggtt catcactttc
    21541 acccatgccg ctacgaagtc tttaacaaac ttctcgtggg catcgctgct ggcgtaaact
    21601 tccgctactg cacgcaggac agagttagaa ccaaatacca gatccgcacg gctggctgtg
    21661 tatttcactt cgccggtttc gcggtcacgt ccttcgaaca gctctttcga ttcgtcagtc
    21721 gctttccact cgtaacgcat atccagcaag ttcacgaaga agtcattgct caatacgcca
    21781 acgcggtcag tgaagacgcc gtttttgctg ccatcgaagt tggcacccag tacacgcatc
    21841 ccgcccacca gcgcagtcat ttccggcgcg gtcagcgtca gttgctgtgc tttgtcgatc
    21901 agcagtgact cggtggtgga aacgtccaga cgagcgcgat agttacggaa accgtcagca
    21961 attggctcca gcagctcaaa catctcaatg tcagtctgat cctgacgcgc atcaacgcga
    22021 cccggcgcaa acggtacatg aatgctcaaa cctgcggcgc ttgcggcttt ctcaacacca
    22081 accacaccag ccagcacgat gatatccgcc agcgaggctt taccagactc tttctggatt
    22141 ttctccagaa caggcagagc acgaacggct gcggcgttca catcccagtc gcgctgcggc
    22201 attaatgcca gacgcgcacc gttggcacca ccgcgtttgt cgccaccacg gaaggtagaa
    22261 gcagatgccc aggccaccga taccagctca ctaacagaca gaccagaatc cgcaatcgcg
    22321 aatttcagat cgataatgtc ctgctcggtc gggttgtaga tcggctgcgg cagcggatct
    22381 tgccagatca gatcttcttt cggcacttcc ggcccgatgt agcgagattt cggccccata
    22441 tccctgtgcg tcagtttgaa ccaggcacgg gcaaaggctt cgttgaacgc ctgcggatcg
    22501 ttgaggaaac gacgagagat cttctcgaac tcaggatcaa aacgcagcgt caggtcggtc
    22561 accagcattg tcggtttacg tttcttcgac ggatcgaacg gatccgggat aatttccggt
    22621 gcgtctaccg cttcgaactg gattgctcca gccggactgc gggtctgtac ccactcatac
    22681 ttgaacagat tctcgaagaa atagttgctc cactggatcg gcgtctgggt ccagactact
    22741 tccagaccag aggtaatggc atctgcgcca acgccgctgc cgtaagtgct cgcccagcct
    22801 aaaccttgtt cttcaatcgg tgcagcttct ggatcaggac ctacgtttga tgtcggaccg
    22861 gcaccgtggg ttttacccag cgtatgacca cccgcaatca gcgccacggt ttcttcgtcg
    22921 ttcatgccca tgttgccgaa ggtcgcgcgg atagctgctg ccgcagaaag cggttcgccg
    22981 ctgtgatccg ggccttccgg gttaacgtaa atcaggccca tctcggttgc acccagcggt
    23041 gctttcgcca gcgcttccgg atgacggtga gtcagccagg ctttttcatc accccagtta
    23101 acatccagat ccggttccca gacgtcttca cgaccggcac caaaaccgaa ggtacggaag
    23161 ccggagtttt ccagcgccac gttacctgcg aggataaaca ggtcggccca ggagattttc
    23221 tgaccatatt tctgtttgat tggccacaac aggcgacgcg ctttatcgag gcttacgtta
    23281 tccggccagg agttcagcgg tgcaaaacgt tgctgaccac gacccgcgcc gccgcgtcca
    23341 tcgattgaac ggtaagtacc tgcgccgtgc caagccatac gaataaacag accggcgtag
    23401 ctgccccagt cggctggcca ccacggctga gattctgtca gcagggcttt cagatctttt
    23461 ttcaggccgt agtaatctaa tttgctgaat tctttgcggt agtcaaagtc ctcacccagc
    23521 gggttagaac gattagaatg ttggtttaac aggtcaacac ggagttgatt tggccaccag
    23581 tcgcgagtgg ttgtgcccgc ccccgcgctc tggtcgtgac cgccctgatg gaacgggcat
    23641 ttgccagtgg ctgtggtgtt atggatatcg tctgacgtgc tcatcaatgt gctcccctct
    23701 acagtgttac cgttacgata cacagcgtta gagagaagtt ataattgaat taatccacgg
    23761 atgcgatagt tgagatctta catattgttg gttaaagaga tgtagatcaa attgatcgta
    23821 attaagcttt tttgattatg tccgaattcg gacaaatgct ttataaaaaa gggttttagc
    23881 ttcatatcct tcaagtaatg agggagaaaa caaggctgac aaccttattt tcccgccctc
    23941 atttcgaggc agcattttgt gctctgttta aaatttgtga tcactgtgtg attttcacaa
    24001 aagccacact atttataaac caggtcgaac acccagcgta tggcaaatcg cgtaactcat
    24061 ttcagcgcgg ttaagcgtat agaagtggaa atctttcact ccttcacggc ttaaaatctt
    24121 caccatatcc atggcaatat tcgcgcccac cagtttgcgg gtttcggcat cgtcatccag
    24181 cccgtcaaac atttgcgcca tccacgccgg aatacgcacg ttggtcatgt cggcaaattt
    24241 cttcgcctgt ttaaagttag ataccggcaa aatgcccggg atgatttcca catcaatgcc
    24301 cgccgataca cagcggtcac gaaaacgcag gtagctttcg acatcgaaga agaactgagt
    24361 aatcgcgcgg ttggctccgg catccacttt gcgtttcaga ttaagcaaat ccgcctgagc
    24421 gctttttgct tccgggtgaa cttccggata cgccgccacg gatatatcga aatctgccac
    24481 ttcttttaac agcgtcacca ggtcagaagc atacatttct ggcttaccac ttcccggcgg
    24541 cagatcgcca cgcagtgcca cgatatgacg aataccgtta ttccagtagt cgcgtgcaat
    24601 ggtgcgcagc tcgtcgggcg tcgcatcaat gcaagtaaga tgcggtgccg cttccagacc
    24661 agtgcgatct ttaatgcctt taataatgct gtgcgtacgg tcgcgctcgc cggagttcgc
    24721 gccataggtc accgatacaa acttcggttt caggctgcta aggcgatcga tggagttcca
    24781 cagggtctgc tccatttcac tggtacgcgg cgggaaaaac tcgaacgaaa cgttaatctg
    24841 cccctggact tctgccaggc tctgattcag ggcatcccgc tggctggcgt gaaaaaagct
    24901 cataccttac ctcatcaatc gcttgtagtt atatgttgtg ttgtgcacat ctatccattt
    24961 agacgtccag atgtaaagat gaaggaaaag ccgaagggcg tcaaccgaaa aatgaccaat
    25021 gatggtgagg aaaaatcagg gaagatgaaa aaacttcagg atggtaagaa aaagaaaatc
    25081 cgatatgcaa agcaaaccgg actgcaaata taaactaacc atgtacttat tgcttcttgc
    25141 cggattcgtc gtaaacgctt catccggcct aattctatga taattcaata tatttcatta
    25201 gaaaccatag acccgaaaac atgagtaccg ggcattatta aatttctgaa attacaacaa
    25261 ctgtgccagc cggttgatat ccgactgaat cgccccggcg gtgacgtcgc gcccagcgcc
    25321 aggcccacgg atcaccagcg gattatcgcg gtaccagcgg ctttcgatgg caaagacgtt
    25381 atcgcatggc agcagtgatg ccagcggata gtcttcacgc accgcctcca cgcccacacg
    25441 cgctttgccg ttggcatcga aacgcgccac gtagcgcagc accagcccca tttcgcgggc
    25501 cgcttccagc cgttgcacca tctgctcgtt aagttcatcg ccattttcaa agaaatggtc
    25561 gatgctgccg ccttcgcaat gagcaggcac cagcgattcc acacgtacct ggtccggttc
    25621 aatgttgtaa cccgcttcac gcgccagaat caccagcttg cgcattacgt ctttgccgga
    25681 gaggtcgtca cgcggatcag gctcggttaa gccctgctgc cacgcctgat ccaccagctc
    25741 ggtaaacggc acgctaccgt cgaattgcag gaacagccag gagagcgtgc cggagaagat
    25801 cccactgatc gacaaaatgg tatcgccgct gtcgatcaga tcgcgcacgg tatggttgat
    25861 cggcaagccc gcaccaacgg tggcgttgta tagccagtga cgcccggttt tttcgaaggc
    25921 gtcgtggatc tggcgatatt tattgctgtc gcttgctccc gccagtttgt tggcactgat
    25981 gacgtggaaa ccgtggctgg cgaaatcgag atactgatca gcaagctgct ggctggcggt
    26041 aacgtccaac accactaaat catcatacgg atgggcgcgc atccacagga aaagcgactc
    26101 ttcatcctgc tcgaccgctt catcgttgaa gaaggctaac gcgcggctgg cgtccagccc
    26161 gtcataactc aacagactgc ggcggctgtc caccacgcct gccagcacaa attcaaagcc
    26221 ggtacgcgcc gaaagcgtgc tctgctcgcg ggcgaacagt tccagccagc gagagccgat
    26281 attgcctttg ccgaacaata ccaggccgat gcgtttttct gcgcggaaga cggactgatg
    26341 cagcccctga atcaggcttt cggtcgggcc ggtgcgcagt actgccacca gactgatgcc
    26401 gtcatcggac tgccacgtaa actcgaccgg ctggcctttc agttgctgcc agaagcggtg
    26461 gcaatgcagc gggttacggg tgacacccgc cccgaccatc gccaccagcg ccagcccctg
    26521 acgcaggcgc agttctccag gtaatcccgc ttcgtcgagg attttcagcg cactgtcggc
    26581 cacttctgag gtgtagcaaa attgcaacaa ctggcgatcg ttatgcacgc caaccgccag
    26641 tggacgcacc tgggcacgtt tcaggatctg gtcgatctct ttatgcgcca gtttgaaatc
    26701 ctgactggcg ggcacctgaa actcaatcaa acagacatca tcgtggctgg tgacaatacg
    26761 cgcaccagta ccggaggcca gcacgcgttc aatgcgcgtg gaaccttgat ccggcgtgta
    26821 gctacagcgc agctgcaggt cgatttcgct gccagaaact ggctgtaaag tacgggcgtg
    26881 aagaacggga gccgccagac gcgccagttc gctggcctca tccagacgca gcaacggcag
    26941 caggcaggca tctttcactt tacgcgggtc ggcactgtat accccggcga cgtcgctcca
    27001 gatggttaca cgagaaacac ccgccagcgc accgatttgt gtcgcggaat agtcggaacc
    27061 gttacgcccc agcaacaccg tttcaccggc gttgttacgg ctgataaacc cggtcaccac
    27121 cagacgtttg ctcggatgtt gtaccagtag ctgttgcagc aacgggtaag aaagcccttc
    27181 atcaacctgc ggttgcgcag cgcgttcagc gcgtaaaaac tcacgggcat caagccaggc
    27241 cgctggcagc ccttgttgat taagtaccgc agacatcagg cgcgccgacc acacttcacc
    27301 gtggcccacc acttccgcat acaccgcatc gttaatacca ctgtcgagca acgccgccag
    27361 gcgctcaaga tcgctgacaa aagcgctaat gaggctatcg gcttcttcag cgggtagcag
    27421 accgctaatc agatcgcact gatagcgacg cagcgtttgt tgaacctgat gcgcagagag
    27481 acgatcggtc tggcttagtt tcaaccagtt aatcaactgg ttagtggtgc taccggcggc
    27541 ggaaaccacc atcatatcgt caggctgaga gaactccgcc ataatgcccg cgacacgcaa
    27601 ataacacttc acatcagcca gactactgcc accaaattta tgcagctgac gacctttcgc
    27661 ccctgcctgc gcaatcacac tcatttttac cccttgtttg cagcccggaa gccattttcc
    27721 aggtcggcaa ttaaatcttc gccatcttca ataccggtgg agatacgcag cagcgtctcg
    27781 gagatcccgg cggcagcacg cgcttctggt gccatgcctg catgtgtcat ggttgcggcg
    27841 tgagagatta aactttccac tccccctaat gattccgcca gcgtaaacaa cgacagcccg
    27901 cccaggaaac gacacagcgt ctgctcatcg ccatccagtt caaaactcaa cattgcgcca
    27961 aagccttttt gctgacgcgc ggcaatttca tgcccctgat tttccggcag cgacgggtga
    28021 tacagttttt tcaccaacgg ctgggtttgc agatatttca caatcgcctg cgcgttgcgc
    28081 tgcgccagct ccatacgcgg caccagcgtt cgcaacccgc gtagcagcag atagctgtca
    28141 aacgcgccgc ccgtcacgcc aatattgttt gcccaccagg caagttcagt gacaacgtcc
    28201 gggtctttag caatcaccac gccagccacc acgtcagagt gaccgttcag atatttcgta
    28261 catgaatgca acaccagatc ggcacccaat gccagcggat tttgtaatgc cgggcttaag
    28321 aaggtgttat ccaccacgct caccgccccg acttcccttg ccagatggca gattttcgca
    28381 atatccacga cgcgtaacaa tggattactt gggctttcta ccagtaccag tttgggtttt
    28441 tctgccagcg ctgcccgtaa tgcctgttca tcgccttgat caacaaacaa cacgcgatag
    28501 caaccgcgtt tcgccagact gtcgaacagg cgatagctac cgccgtagca gtcgtgcggc
    28561 gcaaccagca gatcgccagg tttcaaaaag acggtcgtta ccaggtgaat cgcggacatg
    28621 ccggtattag taagtactgc gccagcaccg ccttccagtt ctgccagcgc acgctgaacc
    28681 acatcgcgcg ttgggttgcc gcgacgcgag taatcatgcg cgcgcggttc attaaatccg
    28741 gtaaagttat aggtgctgga aagatggatg ggagggacaa cgcaaccata ctgttcgtcg
    28801 tcatttaacc cgctacgcac tgcgatggtg gcctgtttac gcgtcatgtg atgaagtttc
    28861 ctgggctttg tcggtgaaat gtcaggcacc agagtaaaca ttgtgttaat ggacgtcaat
    28921 acatctggac atctaaactt ctttgcgtat agattgagca aatcccaaat agccgttaaa
    28981 attatatgca ttatcacgcc gacaggtgca ttacacgatg tcacggtaac gcctgtacgg
    29041 taaactatgc gggtttacgg tcagtaccca cataaattgt ggggtctggt ctcaatttat
    29101 tgacgaagag gattaagtat ctcatggctg aatggagcgg cgaatatatc agcccatacg
    29161 ctgagcacgg caagaagagt gaacaagtca aaaagattac ggtttccatt cctcttaagg
    29221 tgttaaaaat cctcaccgat gaacgcacgc gtcgtcaggt gaacaacctg cgtcacgcta
    29281 ccaacagcga gctgctgtgc gaagcgtttc tgcatgcctt taccgggcaa cctttgccgg
    29341 atgatgccga tctgcgtaaa gagcgcagcg acgaaatccc ggaagcggca aaagagatca
    29401 tgcgtgagat ggggattaac ccggagacgt gggaatacta aagcggaaaa gagcggcgcg
    29461 gagtggaatc gcctgatgca ctacgcttat caggcctacg tcatattgca atttattgaa
    29521 tttgcacgaa cttgtaggcc ggataaggcg ttcacgccgc atccggcata aacaacgagc
    29581 acgttgtctg cgacccaccg ctttttatac atggacgttt aactatgaaa aacaggctgc
    29641 tgatcctcag cctgctggtt tctgtacctg cctttgcctg gcagccgcaa actggcgaca
    29701 tcatctttca aatctctcgc tcatcgcaaa gtaaagcgat ccaactggca acccattccg
    29761 attacagcca caccggtatg ctggtgatgc gtaacaaaaa gccctacgtt tttgaagcgg
    29821 tcggcccggt gaaatacacc ccgctcaagc agtggattgc ccatggtgaa aagggcaact
    29881 acgttgttcg ccgcgttgaa ggcggactga gtgttgaaca acagcaaaaa ctgacgcaaa
    29941 cggcaaaacg ttatctcggt aaaccgtatg atttcagctt ctcatggagc gacgatcgcc
    30001 agtactgttc ggaagtggtg tggaaggttt accagaacgc gctgggaatg cgcgtgggcg
    30061 aacagcagaa gttgaaagag tttgatctca gcaacccgct ggtccaggcg aagctcaaag
    30121 aacgctacgg taaaaatatt ccgcttgagg aaacggtagt ttcaccacaa gccgttttcg
    30181 acgcgccaca actcaccacg gttgccaaag agtggccgct gttttcgtgg tgatacgacc
    30241 aaaattcagg cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg gtcttatttg
    30301 ctgcccggga tgttgaaacg cttgttgaag cggtcaacac ggccaccggt agcaacatca
    30361 cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt caggtcatga
    30421 ccaacggtgg agcggatttt tattacgtta ccgcaagagc agctagcagt aatttcttcg
    30481 tatttcgggt gaatatcttt tttcatggga aaacctcggt ttaaggccgc gtcgctcttc
    30541 cagccctaac gccagacacc acgcgatgtt aaaagtatag cttcaatacg atcatttcgt
    30601 acgaaggcgc gaaatcatac agaaattaac cagcgtatgc aaactgatcc gcactcttct
    30661 acggcaatgt gtatactaac ccaccgaatt tcaagtcagg atgatgctat gcccgttgcc
    30721 cacgttgcct tgcccgttcc gcttcctcgt acctttgact atctgctgcc agaaggcatg
    30781 acggttaaag ctgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga gcgcatcggg
    30841 attgtggtat cagttagcga tgccagcgaa ctgccgctca ctgagctaaa agcggtagtc
    30901 gaagtgctgg atggtgagcc ggtgtttact cactccgtct ggcgattgct gctatgggcg
    30961 gcagattact atcatcatcc gattggcgat gtgctgtttc atgccttgcc gattttacta
    31021 cgccaggggc ggcctgcggc gaacgcgccg atgtggtact ggtttgccac tgaacaaggt
    31081 caggcggtgg atctgaacag cctgaaacgc tcacccaagc aacaacaggc gctggcagcg
    31141 ttacggcaag gcaaaatctg gcgcgaccag gttgccacgc tcgaatttaa tgatgccgcg
    31201 ttgcaggcgc tgcgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc agagtttagc
    31261 gactggcgaa cgaactatgc cgtttctggt gagcggttgc gattgaatac cgaacaggcc
    31321 accgccgttg gcgcaattca tagcgcggca gatacttttt ctgcctggct gctggcgggc
    31381 gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt gctcgctcag
    31441 ggcaaacagg cgctggtgat ggtgccggaa atcggcctga caccgcaaac tatcgcccgt
    31501 tttcgtgaac ggtttaatgc gcccgtggaa gttctgcatt ccggtctgaa cgacagcgag
    31561 cgtctttcgg cgtggctgaa agcgaaaaat ggcgaggcgg cgattgtgat cggcacccgc
    31621 tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg tcattgacga agagcacgac
    31681 agttcctaca agcagcagga aggctggcgc tatcatgccc gcgacctggc ggtgtatcgt
    31741 gcgcatagcg agcaaatccc gattattctt ggctccgcaa cgcccgcgct ggaaacgttg
    31801 tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga cgcgccgggc ggggaatgcg
    31861 cgtccggcaa ttcaacacgt gctggattta aaaggtcaga aggtgcaggc aggtctggct
    31921 ccggcgttaa tcacccgtat gcgccagcat ttacaggcca acaatcaggt tatcctcttt
    31981 cttaaccgcc gtggctttgc gcctgcgcta ctgtgccacg actgcggctg gattgccgaa
    32041 tgcccacgtt gcgatcacta ctacacgctg catcaggcgc aacagcattt gcgctgtcac
    32101 cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt cctgcggttc cacgcacctg
    32161 gtacccgtgg ggctgggcac cgaacagctt gaacagacgc ttgcgccgct gttccctgat
    32221 gtgcccattt ctcgtatcga ccgcgatacc accagccgca aaggggcgct ggaacagcaa
    32281 ctggcggaag tacatcgcgg cggtgcgagg attttgatcg gcacacaaat gctggcgaaa
    32341 ggtcaccact ttccggatgt gacgctggtg gcgttactgg atgtggacgg cgcgctgttt
    32401 tctgccgatt ttcgctcggc agagcgtttc gctcagcttt acacccaggt cgctggtcgt
    32461 gccgggcgtg cgggtaaaca aggcgaagtg gtgctgcaaa cgcaccatcc ggaacatcct
    32521 ctgttgcaaa cgttgctcta taaaggctac gatgcctttg ccgaacaggc gctggctgag
    32581 cgacggatga tgcaactgcc gccatggacc agccatgtga ttgtgcgtgc ggaagatcat
    32641 aacaatcagc atgcgccgct gttcctgcaa caactgcgta atctgatcct ctccagcccg
    32701 ctggcagacg acaaactgtg ggttctcggt ccggttccgg cactggcacc taaacgtggc
    32761 ggtcgctggc gctggcagat attgttgcag cacccttccc gcgtgcgctt gcaacacatc
    32821 attagtggta cgctggcgct catcaataca ataccggatt cccgcaaggt gaaatgggta
    32881 ctggatgttg atccgattga gggttaaacc gcttacgatg cgaggcggat cgaaaaattc
    32941 aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta tctgctaaaa
    33001 atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgtgccagg cgaggagtga
    33061 gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa agacgttgcc ctcaaggcaa
    33121 aagtctctac agcgactgtc tcccgagcat taatgaatcc cgataaagtc tcccaggcca
    33181 cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta tttaccgcag cctatggggc
    33241 gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat atctgcgatc
    33301 ccttctttag cgaaattatt cgcggtatcg aagttacggc ggcaaatcac ggatatctgg
    33361 tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa aacctttatc gatttgatca
    33421 tcaccaagca aattgatggc atgttgctgc tgggttcaag gctgccgttt gatgccagca
    33481 ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa cgaatttgca ccggaactgg
    33541 agctgcctac cgttcatatc gacaatctga ccgccgcatt tgatgcagta aattatttat
    33601 atgagcaagg gcataaacgg attggctgta tagccggtcc cgaagagatg ccactgtgtc
    33661 actaccgcct gcaaggctat gttcaggcgc tgcgtcgctg cggaattatg gttgatccac
    33721 aatacatcgc ccgtggcgac ttcaccttcg aagccggaag caaagcaatg caacagctgc
    33781 ttgatcttcc acaaccgcct actgcagtct tctgccatag cgatgtgatg gcgctcggcg
    33841 cactttctca ggcaaaacgc caggggctga aagtcccgga agacctttcc ataatcggtt
    33901 ttgataacat cgacctgacg caattttgtg atccgccgct gacaaccatc gcgcagccgc
    33961 gttacgaaat cggtcgggaa gctatgctgt tattgcttga tcaaatgcag gggcaacacg
    34021 ttggcagtgg ctctcgttta atggactgcg aacttatcat ccggggatca acacgcgcgt
    34081 taccttaaag taaaccttta agactgccgt gactggtcaa aggcccgccg cttaagtaac
    34141 atggcgggct gacgaacgaa taaatacagc gaaacgatag tggcacaacg agattatgta
    34201 cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca cctcacggaa aaagcaacga
    34261 aatctgcctg cggtttctcc cgctatggtc gctattgctg ccgccgttct tgtgaccttt
    34321 atcggtggtc tgtacttcat tacgcatcac aagaaagaag agtccgagac gctgcaaagc
    34381 cagaaagtga ccggaaacgg actaccacca aaaccagaag aacgctggcg ctacattaaa
    34441 gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc cggtggtgaa
    34501 gtgaaaacgc cggagcaact gacaccagaa caacgtcagc ttcttgaaca aatgcaggct
    34561 gatatgcgcc agcagccaac gcagctggtt gaggttccgt ggaatgaaca gacgccggaa
    34621 cagcgtcagc aaacgctaca acgccagcgt caggcgcagc aattagcgga gcagcaacgc
    34681 ctggctcagc agtcgcgtac gactgaacaa agctggcagc agcagacgcg tacgtcgcaa
    34741 gccgcgccgg tacaggcgca accgcgccag tctaaaccag cttctactca gcagccgtac
    34801 caggatctgc tgcaaacccc tgcgcacacg accgcgcagt cgaagccgca gcaagctgcg
    34861 ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga cgaacgccgc
    34921 tggatggtgc agtgcggttc gttcagaggc gcggaacagg cagagacggt acgtgctcag
    34981 ctggcgttcg aaggatttga ctcgaaaatc accaccaaca atggctggaa tcgtgtggtc
    35041 attggcccgg tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt gaagatggcg
    35101 ggtcatacaa actgcattcg gctcgccgcc gggggttgaa accctcaaaa tcccccccat
    35161 ctataattgc attatgcccc gtacttttgt acggggtttg tactctgtat tcgtaaccaa
    35221 ggggtcagct cgtgacaact atagtaagcg tacgccgtaa cggccatgtg gtcatcgctg
    35281 gtgatggtca ggccacgttg ggcaataccg taatgaaagg caacgtgaaa aaggtccgcc
    35341 gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat gcttttacgc
    35401 tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg ccatctggtt aaagccgccg
    35461 ttgagctggc aaaagactgg cgtaccgatc gcatgctgcg caaacttgaa gcactgctgg
    35521 cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg gtgcagccag
    35581 aaaacgatct tattgctatc ggctccggcg gcccttacgc ccaggctgcg gcgcgcgcgc
    35641 tgttagaaaa cactgaactt agcgcccgtg aaattgctga aaaggcgttg gatattgcag
    35701 gcgacatttg catctatacc aaccatttcc acaccatcga agaattaagc tacaaagcgt
    35761 aaggatctcc catgtctgaa atgaccccac gcgaaatcgt cagcgaactg gataagcaca
    35821 tcatcggcca ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac cgctggcgtc
    35881 gcatgcagct caacgaagag ctgcgccatg aagtgacccc gaaaaatatc ctgatgatcg
    35941 gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg gcgaatgcgc
    36001 cgttcatcaa agttgaagcg accaaattta ccgaagtggg ctacgtcggt aaggaagttg
    36061 attctattat tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc caggctatcg
    36121 agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg tattctcgac gtgctgatcc
    36181 cacctgctaa aaacaactgg ggacagactg aacagcagca ggaaccttcc gctgctcgtc
    36241 aggcattccg caaaaaactg cgtgaaggcc agcttgatga caaagaaatc gagatcgatc
    36301 ttgccgcagc accgatgggc gttgaaatta tggctcctcc gggcatggaa gagatgacca
    36361 gccagctgca gtccatgttc cagaacctgg gcggccagaa gcaaaaagcg cgtaagctga
    36421 aaatcaaaga cgccatgaag ctgctgattg aagaagaagc ggcgaaactg gtgaacccgg
    36481 aagagctgaa gcaagacgct atcgacgctg ttgagcagca cgggatcgtg tttatcgacg
    36541 aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc ggatgtttct cgtgaaggcg
    36601 ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa cacgggatgg
    36661 tcaaaactga ccacattctg tttatcgctt ctggcgcgtt ccagattgcg aaaccgtctg
    36721 acctgatccc ggaactgcaa ggtcggctgc caatccgcgt tgaactgcag gcactgacca
    36781 ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg cagtacaaag
    36841 cactgatggc gactgaaggc gtaaatatcg agtttaccga ctccggtatt aaacgcatcg
    36901 cggaagcggc atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt cgtttacaca
    36961 ctgttctgga gcgtttaatg gaagagattt cctacgacgc cagcgattta agcggtcaaa
    37021 atatcactat tgacgcagat tatgtgagca aacatctgga tgcgttggtg gcagatgaag
    37081 atctgagccg ttttatccta taatcgcgtt caatcatttt catcattgtt tgatggggct
    37141 gaaaggcccc atttttattg gcgcgtatta tgactgaaca acaaattagc cgaactcagg
    37201 cgtggctgga aagtttacga cctaaaaccc tccccctcgc ctttgctgca attatcgtcg
    37261 ggacggcgct ggcatggtgg caaggtcact tcgatccgct ggtcgccctg ctggcactaa
    37321 ttaccgccgg gctattacag atcctttcta acctcgccaa tgattacggc gatgcggtaa
    37381 aaggcagcga taaacctgac cgcattgggc cgctacgcgg catgcaaaaa ggggtcatta
    37441 cccagcaaaa gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt ctctccgggc
    37501 tggcactggt tgcagtggca tgccatacgc tggccgattt tgtcggtttc ctgattcttg
    37561 gcgggttgtc gatcattgcc gctatcacct acaccgtggg caatcgtcct tatggttata
    37621 tcggtctggg tgatatttcc gtactggttt tctttggctg gttgagtgtc atggggagct
    37681 ggtatttaca ggctcataca ttgattccgg cactgatcct tccggcgacc gcatgcggcc
    37741 tgctggcaac ggcagtactg aatattaata acctgcgtga tatcaatagc gaccgcgaaa
    37801 atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt tatcatgcct
    37861 gcctgctgat gggctcgctg gtgtgtctgg cgctgtttaa tctcttttcg ctgcatagcc
    37921 tgtggggctg gctgttcctg ctggcggcac cattactggt gaagcaagcc cgttatgtga
    37981 tgcgggaaat ggacccggtg gcgatgcgac caatgctgga acgtactgtc aagggagcgt
    38041 tactgactaa cctgctgttt gttttaggga tattcctaag ccagtgggca gcataactga
    38101 caaatatcaa ttaacaattg atgattttgc caacagccca catagcgcga tatactgaaa
    38161 attctcgcag caactgaatg ttaagcctat gaaatacgat acttccgagc tttgtgacat
    38221 ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc aactttggcg gacgggcgtc
    38281 gtttggcgga caaataatca cggtaaaatg tttcgaggac aacgggttgc tgtacgatct
    38341 gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt ctgttcgtcg
    38401 cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg aaggtctggt
    38461 catttacggc gcggtgcgtc aggtagatga cctggaagag ttggatatcg gcatccaggc
    38521 gatggcggca attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg atgtccgcgt
    38581 caattttggc ggtgtcacct tcttctccgg cgaccatctt tatgccgaca ataccgggat
    38641 tattctttca gaagatccgc tggatattga atgataagaa aggcaccgca aggtgccttt
    38701 tttctgcgtt acctgttggc ctacacagta aagaaattac gcgaaagatg aagcgtaatc
    38761 agacctcttc catgcgaccc agcagggcct gcagacgttc ctgccagccg ttctgctgtt
    38821 ctttcagatg gttgttctca cgctccagct cttcgcgctg atgctgggca ttttgaactt
    38881 cctgcgacag tgagttgttt ttttctttca gctcttcgat ttccatctgc aacagagtga
    38941 tggtatcaat cgcctgctgt acttttgctt ccagtttctc aaacacttct aatgacattg
    39001 tcatacctct cctgaattgc aaggcgttga tggataaaaa atcctcgtcc cgattaccgg
    39061 tgacgcctta ataaatacga gcgcacttta gttagctccg attgtatgaa gccgcgccat
    39121 cgctgtccag cggcacgcct tgcagattac ggtttgccac acttttcatc cttctcctag
    39181 tgacataatc cacatcaatc gaaaatgtta ataaatttgt tgcgcgaatg atctaacaaa
    39241 catgcatcat gtacaatcag atggaataaa tggcgcgata acgctcattt tatgacgaag
    39301 cacacacatt ttaagttcga tatttctcgt ttttgctcgt taacgataag ttaacagcat
    39361 gcctacaagc atcgtggagg tccgtgactt tcacgcatac aacaaacatt aactcttcag
    39421 gatccgatta tgagtcaaac atcaaccttg aaaggccagt gcattgctga attcctcggt
    39481 accgggttgt tgattttttt cggtgtgggt tgcgttgcag cactaaaagt cgctggtgcg
    39541 tcttttggtc agtgggaaat cagtgtcatt tggggactgg gggtggcaat ggccatctac
    39601 ctgaccgcag gggtttccgg cgcgcatctt aatcccgctg ttaccattgc attgtggctg
    39661 tttgcctgtt tcgacaagcg caaagttatt ccttttatcg tttcacaagt tgccggcgct
    39721 ttctgcgctg cggctttagt ttacgggctt tactacaatt tatttttcga cttcgagcag
    39781 actcatcaca ttgttcgcgg cagcgttgaa agtgttgatc tggctggcac tttctctact
    39841 taccctaatc ctcatatcaa ttttgtgcag gctttcgcag ttgagatggt gattaccgct
    39901 attctgatgg ggctgatcct ggcgttaacg gacgatggca acggtgtacc acgcggccct
    39961 ttggctccct tgctgattgg tctactgatt gcggtcattg gcgcatctat gggcccattg
    40021 acaggttttg ccatgaaccc agcgcgtgac ttcggtccga aagtctttgc ctagctggcg
    40081 ggctggggca atgtcgcctt taccggcggc agagacattc cttacttcct ggtgccgctt
    40141 ttcagcccta tcgttggcgc gattgtaggt gcatttgcct accgcaaact gattggtcgc
    40201 catttgcctt gcgatatctg tgttgtggaa gaaaaggaaa ccacaactcc ttcagaacaa
    40261 aaagcttcgc tgtaatatga ctacgggaca attaaacatg actgaaaaaa atatatcgtt
    40321 gcgctcgacc agggcaccac cagctcccgc gcggtcgtaa tggatcacga tgccaatatc
    40381 attagcgtgt cgcagcgcga atttgagcaa atctacccaa aaccaggttg ggtagaacac
    40441 gacccaatgg aaatctgggc cacccaaagc tccacgctgg tagaagtgct ggcgaaagcc
    40501 gatatcagtt ccgatcaaat tgcagctatc ggtattacga accagcgtga aaccactatt
    40561 gtctgggaaa aagaaaccgg caagcctatc tataacgcca ttgtctggca gtgccgtcgt
    40621 accgcagaaa tctgcgagca tttaaaacgt gacggtttag aagattatat ccgcagcaat
    40681 accggtctgg tgattgaccc gtacttttct ggcaccaaag tgaagtggat cctcgaccat
    40741 gtggaaggct ctcacgagcg tgcacgtcgt ggtgaattgc tgtttggtac ggttgatacg
    40801 tggcttatct ggaaaatgac tcagggccgt gtccatgtga ccgattacac caacgcctct
    40861 cgtaccatgt tgttcaacat ccataccctg gactgggacg acaaaatgct ggaagtgctg
    40921 gatattccgc gcgagatgct gccagaagtg cgtcgttctt ccgaagtata cggtcagact
    40981 aacattggcg gcaaaggcgg cacgcgtatt ccaatctccg ggatcgccgg tgaccagcag
    41041 gccgcgctgt ttggtcagtt gtgcgtgaaa gaagggatgg cgaagaacac ctatggcact
    41101 ggctgcttta tgctgatgaa cactggcgag aaagcggtga aatcagaaaa cggcctgctg
    41161 accaccatcg cctgcggccc gactggcgaa gtgaactatg cgttggaagg tgcggtgttt
    41221 atggcaggcg catccattca gtggctgcgc gatgagatga agttgattaa cgacgcctac
    41281 gattccgaat atttcgccac caaagtgcaa aacaccaatg gtgtgtatgt ggttccggca
    41341 tttaccgggc tgggtgcgcc gtactgggac ccgtatgcgc gcggggcgat tttcggtctg
    41401 actcgtgggg tgaacgctaa ccacattata cgcgcgacgc tggagtctat tgcttatcag
    41461 acgcgtgacg tgctggaagc gatgcaggcc gactctggta tccgtctgca cgccctgcgc
    41521 gtggatggtg gcgcagtagc aaacaatttc ctgatgcagt tccagtccga tattctcggc
    41581 acccgcgttg agcgcccgga agtgcgcgaa gtcaccgcat tgggtgcggc ctatctcgca
    41641 ggcctggcgg ttggcttctg gcagaacctc gacgagctgc aagagaaagc ggtgattgag
    41701 cgcgagttcc gtccaggcat cgaaaccact gagcgtaatt accgttacgc aggctggaaa
    41761 aaagcggtca aacgcgcgat ggcgtgggaa gaacacgacg agtaatgtca ggcacctgaa
    41821 tcgattgcct gatgctgatc gcctgatgcg acgcttacgc gtcttatcag gcctacgagc
    41881 caatgccatt ttgtgtaggc gggataaggc gttcacgccg catccggcat cacgcagact
    41941 ctgaaaccgt gcgggggcaa ccccgcacac atcaataatc ccacccttcc cctgtgctac
    42001 acttcgcgcc attccttact gcttagagtt tgctatgaga cgagaacttg ccatcgaatt
    42061 ttcccgcgtc accgaatctg cggcgctggc tggctacaaa tggttaggac gcggcgataa
    42121 aaacaccgcg gacggcgcgg cggtaaacgc catgcgtatt atgctcaacc aggtcaacat
    42181 tgacggcacc atcgtcattg gtgaaggtga aatcgacgaa gcaccgatgc tctacattgg
    42241 tgaaaaagtc ggtactggtc gcggcgacgc ggtagatatt gctgttgatc cgattgaagg
    42301 cacgcgcatg acggcgatgg gccaggctaa cgcgctggcg gtgctggcag taggcgataa
    42361 aggctgcttc ctcaatgcgc cggatatgta tatggagaag ctgattgtcg ggccgggagc
    42421 caaaggcacc attgatctga acctgccgct ggcggataac ctgcgcaatg tagcggcggc
    42481 gctcggcaaa ccgttgagcg aactgacggt aacgattctg gctaaaccac gccacgatgc
    42541 cgttatcgct gaaatgcagc aactcggcgt acgcgtattt gctattccgg acggcgacgt
    42601 tgcggcctca attctcacct gtatgccaga cagcgaagtt gacgtgctgt acggtattgg
    42661 tggcgcgccg gaaggcgtgg tttctgcggc ggtgatccgc gcattagatg gcgacatgaa
    42721 cggtcgtctg ctggcgcgtc atgacgtcaa aggcgacaac gaagagaatc gtcgcattgg
    42781 cgagcaggag ctggcacgct gcaaagcgat gggcatcgaa gccggtaaag tattgcgtct
    42841 gggcgatatg gcgcgcagcg ataacgtcat cttctctgcc accggtatta ccaaaggcga
    42901 tctgctggaa ggtattagcc gcaaaggcaa tatcgcgact accgaaacgc tgctgatccg
    42961 cggcaagtca cgcaccattc gccgcattca gtccatccac tatctggatc gcaaagaccc
    43021 ggaaatgcag gtgcacattc tctgagtgat ttgatcgatt gagccttcca gtccttcggg
    43081 actggaattt ttttgttcgg agaacgaaga taaggcaagt caatcaaaac aggagaaaaa
    43141 catggctgat tgggtaacag gcaaagtcac taaagtgcag aactggaccg acgccctgtt
    43201 tagtctcacc gttcacgccc ccgtgcttcc gtttaccgcc gggcaattta ccaagcttgg
    43261 ccttgaaatc gacggcgaac gcgtccagcg cgcctactcc tatgttaact cgcccgataa
    43321 tcccgatctg gagttttacc tggtcaccgt ccccgatggc aaattaagcc cacgattggc
    43381 ggcactgaaa ccgggcgatg aagtgcaggt ggttagcgaa gcagctggct tctttgttct
    43441 ggatgaagta ccggactgcg aaacgctatg gatgctggca accggtacag cgattggccc
    43501 ttatttatcg attctgcaac taggcaaaga tttagatcgc ttcaaaaatc tggtcctggt
    43561 tcacgccgca cgttatgccg ccgacttaag ctatttgcca ctgatgcaag aactggaaaa
    43621 acgctacgaa ggcaaactgc gcattcagac ggtggtcagt cgggaaacgg cagcggggtc
    43681 gctcaccgga cggataccgg cattaattga aagtggggaa ctggaaagcg cgattggcct
    43741 gccgatgaat aaagaaacca gccatgtgat gctgtgcggc aatccacaga tggtgcgcga
    43801 tacacaacag ttgctgaaag agacccggca gatgacgaaa catttacgtc gccgaccggg
    43861 ccatatgaca gcggagcatt actggtaagc ggttacttat cgataaacgg cacgatgagc
    43921 aaatccgcac tcatcttatt gatcatcccg cgatatgccg gcatcaaacg gttgataaat
    43981 gagtgatgat gaccacagac aaggaggtcg cactgctctt tttgcataat ttccagcagt
    44041 gtttccggca tttctccgcg ttcaatacgc agttttgtct tcggccattg aatatttttc
    44101 gtcagtttat acagcttgtt atccgactta ttcttcaaca attgaagaat atcttctgtt
    44161 gcagggaagt agatacccgg atacaactcg cttaagccat catcaatatg aattaacgtc
    44221 aggtgagcgt cattatgtct ggcgagctcc agggctttat tcaccagtaa ggcatcttct
    44281 tcattcccgg aaattgccac gccaatgtgt ttataagcca tgtttaactc cttctaaagc
    44341 aactccatca agctaacgaa cgcagtgata actcaaaaat aatcatctcg gcctggcatg
    44401 agaaagtgaa ggccgcatca agctcaactt gcccctccag atccttaatc ttataggcca
    44461 cgctggcccc ttcatctgca gaggatgcag cggcggcaag ctctttcagt ttggtcataa
    44521 aagactctgc ttcttcgcgg gtggcaaaaa agcgcgaaaa cttactggta cagttgtcgt
    44581 tatcaatcac cgtaccgata tcaatcgcac atcctttagt actgcattta tctacgacat
    44641 ctttcatagg gcacctctgt atattcgcct ctctgtcgat tacgacttaa gaataatgcg
    44701 ccgttaaggg aagaacaaac aagtaatgat cttatggatt accatttttt ttgatattgc
    44761 ttaaggtgaa gagttcagaa ggattttccg ggtattgtca tttgcatcgt ggaaataaca
    44821 atcccggaaa aaaatagcag taagagatat attaaaggtt aattacttga tttattgtcg
    44881 gctttatact tcacatcctg agtatcttta ccatatttat tttcgccttg tgtgccaaca
    44941 aacgcaccaa gatcgataag catcatcacc agaatcaacg tcgggacaaa acgccccacc
    45001 gcccattgcc agacacccgg taaaatcgct cagttacccg ccagcagcat ccacgccaca
    45061 atcatcagaa atgcccatgc gccggaacgc ccgcgatcat gcaagcgctt aacagttact
    45121 gccgctgttg gccagagcaa gcacacaagg caaaacgccg cggtctgaat atcgagtaga
    45181 ttcttacccg ccagtgagaa aagcaccagc atgcctgcga accacaggcc tatccaaatc
    45241 cagaaatcac ggcgtccaat acgcccttta aatgagaata accattgctg tatggtcatg
    45301 taagttcctt gatggttgtc ttttccagga ttctaccctt ttgacaaggg cgacagatat
    45361 cgttttaatc ggagccagtc ataacaaaag gtactgtcaa tgaagccagg gtgtacgctg
    45421 ttttttctct tatgttctgc attaaccgtt acaacaacgg cgcatgcaca aacaccagat
    45481 acggcaacga ccgcgcctta tctgctggct ggagccccta ctttcgatct ctccatcagc
    45541 cagtttcgag aagactttaa cagccagaat cccagcctgc cactgaacga atttcgtgcc
    45601 atcgacagca gtcccgacaa agccaatctc actcgtgctg caagtaaaat taacgagaac
    45661 ttgtatgctt ctacagcgct ggagcgcggt accttaaaaa tcaaaagcat tcaaatgacc
    45721 tggctaccca tccaggggcc agagcaaaaa gcagcgaaag cgaaagctca ggaatacatg
    45781 gcagcagtga tccgcacact caccccatta atgaccaaaa cacaaagcca gaaaaaactg
    45841 cagtcgctac taacggcggg gaaaaacaaa cgttattaca ccgagacaga aggtgcactg
    45901 cgttatgttg tcgcggacaa cggcgaaaag gggctgacct tcgctgttga accgattaag
    45961 ctggcgctat ctgaatcgct tgaaggtttg aataaatgac aaaaagcaaa gcctttgtgc
    46021 cgatgaatct ctatactgtt tcacagacct gctgccctgc ggggcggcca tcttccttta
    46081 ttcgcttata agcgtggaga attaaaatgc gacatccttt agtgatgggt aactggaaac
    46141 tgaacggcag ccgccacatg gttcacgagc tggtttctaa cctgcgtaaa gagctggcag
    46201 gtgttgctgg ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat atggcgaagc
    46261 gcgaagctga aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg aacctgtccg
    46321 gcgcattcac cggtgaaact tctgccgcta tgctgaaaga catcggcgct cagtacatca
    46381 tcatcggcca ctctgaacgt cgtacttacc acaaagagtc tgacgaactg attgcgaaaa
    46441 aattcgcggt gctgaaagag cagggtctga ctccggttct gtgcatcggt gaaaccgaag
    46501 ctgaaaacga agcgggaaaa actgaagaag tttgcgcacg tcagatcgac gcggtactga
    46561 aaactcaggg tgctgcggca ttcgaaggtg cggttatcgc ttacgaacct gtatgggcaa
    46621 tcggtactgg caaatctgca actccggctc aggcacaggc tgttcacaaa ttcatccgtg
    46681 accacatcgc taaagttgac gctaacatcg ctgaacaagt gatcattcag tacggcggct
    46741 ctgtaaacgc gtctaacgct gcagaactgt ttgctcagcc agacatcgac ggcgcgctgg
    46801 ttggcggtgc ttctctgaaa gctgacgctt tcgcagtgat cgttaaagct gcagaagcgg
    46861 ctaaacaggc ttaagtctga caggtgccgg atttcatatc cggcacttac tttccttaac
    46921 tcctcgcctt aacgcaaaat ctcacactca tgatcctgga tttcctcggc tgaagcacgg
    46981 ttcagcgtca gtagatttcg ttgtgtcgcc agcaacacaa acgaattatc actctgccgt
    47041 accatcgcca gcccgtagct tcccatatgt tcccgcgcct caggcacttc ttctgcaagc
    47101 atcataaatg ggctgcgttg taccagttcg ctttccgtta cccgacgcgc caggtattca
    47161 tgcccgcgca aaccacctgg cagtggcaac cagcggctgc tgatgttcgc cagattttta
    47221 tccagttgtt cgcgcacatc aggacgaata caagagatat gaatatgaaa atggttttgc
    47281 gtatgcccgg tgcgggagtt gatcgccaaa gaaaccgcgc gatcgggaac cggctggccg
    47341 tattttttgc tcataaaatc acgcgcctgc caggccaacc aaaagaagtt cggcgttgaa
    47401 ggatcggtca acaaaggact ttcagtaccg ttaatacgat acgttggcat caacagatat
    47461 tgcagtggac cattaagatc ttttaaaacc acgtatccgg cattgggttt gacttccgca
    47521 catggcgaag gattttgatt ttgctgctga ttgggcaaac attcctcaag gacaatctta
    47581 cgtaatgtat ccgactcttc accggttaat ttccagtaac caataccggc agccacaacg
    47641 gcgataacta tcatcaccaa aaaaagaaga cccgcttttt tcatcttttt ttccctgtac
    47701 ctcaaagaga tgcaagggta acgcaaaatc gtgacaaata aaaaacccgg tcgcgagaaa
    47761 ccaccgggtt tgataattat cctagggaaa tcagcgtttg ctgatctgat cgaacgtacc
    47821 gccgttagca aaatgctctt tttgcgcttt cgtccagccg ccgaactctt catcaatggt
    47881 gaataacttc agctttggaa acgcattttc gtactttttc gctacttcag cgtcgcgcgg
    47941 acggtagtag tttttcgcgg caatttcctg accttctggc gagtagagat atttcaggta
    48001 ggcttccgcc acctctttgg tgcctttttt ctcgaccact ttatcgacca ccgacaccgt
    48061 tggctctgcg aggatagact cactcggcgt gacgatttcg aatttatctt tccccagttc
    48121 attcgctgcc agcagagctt cgttttccca ggcgatcagt acatcgccaa tcccgcgctc
    48181 gacaaaggtg ttagtggagc cgcgcgcgcc agaatccaga acttcgacgt ttttatacag
    48241 tgcccgaaca aaatcctgtg cttttgcctg atcgttgttg ttgtgatgca gcgcgtagcc
    48301 ccaggcagcc aggtagttcc agcgtgcgcc accagagctt ttcggattag gcgtgatcac
    48361 cgaaacaccc ggtttaatca gatcgttcca gtcatggatc tgcttcggat tgcccttacg
    48421 caccaggaaa acaatggtgg aagtgtacgg tgcggagtta tccggcagac gtttgatcca
    48481 ctctttatca atccgcccgc gttccgcaat cgcgtccacg tcataggcca gagccagcgt
    48541 gacaacatca gcttcaatac cgttgattac cgacgtcgct tgtttacctg aaccaccgtg
    48601 tgactgacga atcaccacgt tatcaccagt ttgctgtttc cagtgggcgc tgaatgcctt
    48661 gttgtactgt tcgtacaatt cgcgcgttgg atcatatgaa acgttaagaa gctgaatatc
    48721 ctttgccata acgctggttg ccgccagcaa aaatgttaac cctacgcccc acttgttcat
    48781 cgcccgactc tcttatgttg tgttgtgatg agcaaagcgt gccagaaggt taaccaaaca
    48841 ttaaagaata aaaaaagatt ggctataact tgcgggtata tgttgaggga ttaaaaaggc
    48901 ggaagaaatc cgcctcatat tgctgacaaa gtgcgctttg tccatgccgg atgcggcgcg
    48961 aacgccttat ccggcctaca aaagtttgca aattcaataa attgcagaat tcatgtaggc
    49021 ctgataagcg aagcgcatca ggcatttttg cttctgtcat cggtttcagg ctaaaggaat
    49081 ctgccttttt ccgaaatcat taatacagtt ttttcgcgca gtccagccag tcacctttga
    49141 acggacgctt catgttttcg atagcgtcga tgatgtcgtg gtgaaccagc tgttcgttct
    49201 ggatacctac acaacgaccg ccgtaacctg ccagcagcag atcgatagcg taagcgccca
    49261 tacgggaagc cagaatacgg tcgtaaggca ccggagaacc accgcgctgg atgtggccca
    49321 gcacagttgc gcgggtttca cgaccggttt ctttctcgat gaaatgcgcc agttcgtcaa
    49381 catcacacat atgttcggta atcgccacga tcgcgtgttt tttacctttc gcgatacccg
    49441 ctttgatttc gtttaccagg tcttcacggc tgaattcaac ttccggaact acaacgaatt
    49501 cacagccacc ggcaatggcc gcagccaggg tcagatcgcc acaataacgg cccatcactt
    49561 ccaccacgga aatacgctgg tgagaagaag aggtgtcacg cagacggtcg atcgcttcta
    49621 caacggtgct cagcgcagtg aagaaaccga tagtgtagtc agtgcctttg atgtcgttgt
    49681 cgatagtgcc cggcagaccg atgcacggga agcccatttc ggtcagacgc attgcaccca
    49741 tgtaggaacc gtcaccgccg ataaccacca gcgcgtcgat accacgtttt ttcaggtttt
    49801 cgatagccac ggcgcggatg ttctcgtcgc ggaattccgg gaaacgcgca gaaccgagga
    49861 acgtaccgcc acggttgatc atgtcagaca cgctgtaacg gtctagctgt accatacggt
    49921 cttcatacag acccagatag ccgtcataaa tacccattac ttccagacct tctgtcagcg
    49981 cagaacgaac aaccccgcga attgcggcgt tcatgcctgg cgcatcaccg ccgcttgtca
    50041 acacacctat tttcttaatc atgactacct ctgaactttg gaatgcaaaa tgaaatctgt
    50101 tgccggaagt cttcttgcac atcgaagtga tccaacgaat gtgcaaatag tataacaatc
    50161 acttcctgct gaattgattc aggtcaggcc aaatggcggt attttataca caaaatgcgg
    50221 gtctggctct cttttatact gattatgaaa gcatagaccg tttaccctcc ctgggtacga
    50281 cggaacaggg gtcctgatgg ataatcacat ccgatcccgg aaaacgccgt aaaatagcct
    50341 gctctacctg atccgccacc atatgtgcct gaaccaaagg cagagagtct tccatttcca
    50401 aatgaatctg aataaagcgg gtcggccctg actgccgcgt gcgaagatcg tgagcgccgc
    50461 taacacccgg ccaggaagtc acgatatcaa taatttcttg ccgttcttca tcaggcaatg
    50521 cgcgatccag taatgactgt accgcctcat atcccatgcg taacgcgcta tataaaatat
    50581 agatgccgat tcccaatgca aacagagcat cggcgcgatg ccagccgtac caagacaacc
    50641 ccagcgccag cagaattgcg ccgttcatca taacatcaga ctggtaatgt agcatatcag
    50701 cccgcaccgc ctggctttgc gtccggcgca ccacccaccg ctgaaacgag acaaggataa
    50761 tcgtacaaat tagcgccaca attgtcacga taaccccgac gcctggatct gtcatcggtg
    50821 ttggagatac cagatgttga atacccgtca aaaacaggaa tagtgccgaa ccggagataa
    50881 acatactttg cgccagcgcc gcgagggact cagctttacc gtgaccaaac gagtgattat
    50941 cgtcggcagg ttgcagggaa tatcgcacca ccagtaaatt cgtcaacgac gcgccgatat
    51001 ccaccagcga atccaccagc gcggcgagaa tactcaccga cccggtatac caccatgcaa
    51061 aaattttaat cagcaatagc aacgaagcca tcgccgtcgc agcaatcgcc gcccgactga
    51121 ccagccgtcc ataagattga ttcataaata ctcccgctat caactgacgc tagtataacg
    51181 gaagcaaatc atctgcaatg cattaagcag caggcaaatt gaggataaaa aaaaccccac
    51241 atcatgtggg ggaagacagg gatggtgtct atggcaagga aaacagggtt tactactggg
    51301 aacgtgagtt gctactactc aatagcttca acgatgaact tttttgccat tgcgtcacgt
    51361 cacgcaactg ctccattcgt tgttgatgtt tctcgtttaa aaccgcttgc tgctccggcg
    51421 ttaacaggcg atacatttgg ttgcggactt ttgccatctc aacctgacga gcaatttgct
    51481 cattcgccat tttttctgcc tgtgcgcgca cagcgttttc atcaaaattt tctgcggtga
    51541 caaggcgatg cattgtctcc agttcgctaa cattaacagg aggctgttcg tgccgggcct
    51601 gttgcataag atctcgcatc tgctgacgct gatgttcggt taaacttatg ccgtcgaaca
    51661 tatggctctg cgtactgcgc tgcgtaagtt cttcacccgg atgccagtta tcgcctgaac
    51721 cgacttcagc agcgtggctt aatgaactga ctgccagcgt tgaggccatg acggcagcgg
    51781 taactatgcg catcatttgc tcccaaaatc tttctgtcgc gattcaacga tagagagttt
    51841 acgattcagg ctgcaaacat gcgtcagggg gtgtaaaaca acgtaaagtc atggattagc
    51901 gacgtctgat gacgtaattt ctgcctcgga ggtatttaaa caatgaataa aatcctgtta
    51961 gttgatgatg accgagagct gacttcccta ttaaaggagc tgctcgagat ggaaggcttc
    52021 aacgtgattg ttgcccacga tggggaacag gcgcttgatc ttctggacga cagcattgat
    52081 ttacttttgc ttgacgtaat gatgccgaag aaaaatggta tcgacacatt aaaagcactt
    52141 cgccagacac accagacgcc tgtcattatg ttgacggcgc gcggcagtga acttgatcgc
    52201 gttctcggcc ttgagctggg cgcagatgac tatctcccga aaccgtttaa tgatcgtgag
    52261 ctggtggcac gtattcgcgc aatcctgcgc cgttcgcact ggagcgagca acagcaaaac
    52321 aacgacaacg gttcaccgac actggaagtt gatgccttag tgctgaatcc aggccgtcag
    52381 gaagccagct tcgacgggca aacgctggag ttaaccggta ctgagtttac cctgctctat
    52441 ttgctggcac agcatctggg tcaggtggtt tcccgtgaac atttaagcca ggaagtgttg
    52501 ggcaaacgcc tgacgccttt cgaccgcgct attgatatgc acatttccaa cctgcgtcgt
    52561 aaactgccgg atcgtaaaga tggtcacccg tggtttaaaa ccttgcgtgg tcgcggctat
    52621 ctgatggttt ctgcttcatg ataggcagct taaccgcgcg catcttcgcc atcttctggc
    52681 tgacgctggt gctggtgttg atgttggttt tgatgttacc caagctcgat tcacgccaga
    52741 tgaccgagct tctggatagc gaacagcgtc aggggctgat gattgagcag catgtcgaag
    52801 cggagctggc gaacgatccg cccaacgatt taatgtggtg gcggcgtctg ttccgggcga
    52861 ttgataagtg ggcaccgcca ggacagcgtt tgttattggt gaccaccgaa ggccgcgtga
    52921 tcggcgctga acgcagcgaa atgcagatca ttcgtaactt tattggtcag gccgataacg
    52981 ccgatcatcc gcagaagaaa aagtatggcc gcgtggaact ggtcggtccg ttctccgtgc
    53041 gtgatggcga agataattac caactttatc tgattcgtcc ggccagtagt tctcaatccg
    53101 atttcattaa cttattgttt gaccgcccgc tattactgct gattgtcacc atgttggtca
    53161 gtacgccgct gctgttgtgg ttggcctgga gtctggcaaa accggcgcgt aagctgaaaa
    53221 acgctgccga tgaagttgcc cagggaaact tacgccagca cccggaactg gaagcggggc
    53281 cacaggaatt ccttgccgca ggtgccagtt ttaaccagat ggtcaccgcg ctggagcgca
    53341 tgatgacctc tcagcagcgt ctgctttctg atatctctca cgagctgcgc accccgctga
    53401 cgcgtctgca actgggtacg gcgttactgc gccgtcgtag cggtgaaagc aaggaactcg
    53461 agcgtattga aaccgaagcg caacgtctgg acagcatgat caacgatctg ttggtgatgt
    53521 cacgtaatca gcaaaaaaac gcgctggtta gcgaaaccat caaagccaac cagttgtgga
    53581 gtgaagtgct ggataacgcg gcgttcgaag ccgagcaaat gggcaagtcg ttgacagtta
    53641 acttcccgcc tgggccgtgg ccgctgtacg gcaatccgaa cgccctggaa agtgcgctgg
    53701 aaaacattgt tcgtaatgct ctgcgttatt cccatacgaa gattgaagtg ggctttgcgg
    53761 tagataaaga cggtatcacc attacggtgg acgacgatgg tcctggcgtt agcccggaag
    53821 atcgcgaaca gattttccgt ccgttctatc ggaccgatga agcacgcgat cgtgaatctg
    53881 gcggtacagg attggggctg gcgattgttg aaaccgccat tcagcagcat cgtggctggg
    53941 tgaaggcaga agacagcccg ctgggcggtt tacggctggt gatttggttg ccgctgtata
    54001 agcggagtta aactccgcat ttgtaggcag gataaggcgt ttacgccgca tccggcattt
    54061 gagcaggatg cctgatgcga cgctgatagc gtcttatcag gcctacactc cgggggttat
    54121 tttccccaca accggcggga aaaatcttcg atcttgccgc tcagtcggcg cttttgcatc
    54181 gtccgcgtcc agcttttcga taacccggcg gcggagagta aacggtgata ctgatcgtca
    54241 tcaaacggca tatgccatgc gatggcggca gcctcctgca cggtcacatc gctgacacgg
    54301 gaaaccaact ccagcggcgc gtccgcagac actttccctg gcgctatcac gctatacaac
    54361 caccccacct tacctgtgtt ttgcatcaac tgcgctatat cgctgatatc aaaatggtaa
    54421 ttgagtttat agcaaggcga gcgcggctgg ctgacctgaa ttaatgcctc tccccagcgg
    54481 aaaatatcgc ccatatgaac attactttcc gtgagaccgt cggttgagag attttcgcca
    54541 aacgcaggcg caacaaacaa ctccgcctgt tccggaaatt cccgcaccca gtagagatag
    54601 tgctcacgag gataatgaca cagcgctctg tctggcccgc cgtgaacctt tttctccgcc
    54661 tgctcgtcac cttccagccc cagctccgtc agcatcaact caccatcaac ctggattttg
    54721 gcaattgcgc tgggtttgcc ttcgggataa gcctgaatct tgcctgtgta tacatcaacc
    54781 ggatatcgca tctgttccct ctccacgaga aatttcccca ttagaccata tttattttat
    54841 gtcagccgtg ctgttttcgc ggtaagtatc caggcaggaa ctcaatagca aaccggacga
    54901 acgttagttc atccggctgt gggga
//
LOCUS       DAJTRH010000017        53217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTRH010000017 DAJTRH010000000
VERSION     DAJTRH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(11..322)
                     /locus_tag="OO339_001105"
     CDS             complement(11..322)
                     /locus_tag="OO339_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5925817.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO339_001106"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO339_001106"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO339_001107"
     CDS             701..1054
                     /locus_tag="OO339_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5925818.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO339_001108"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO339_001108"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5925819.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAKGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO339_001109"
     CDS             complement(2810..3280)
                     /locus_tag="OO339_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5925820.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO339_001110"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO339_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO339_001111"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO339_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5925821.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO339_001112"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO339_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5925822.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5282..8431)
                     /locus_tag="OO339_001113"
     CDS             complement(5282..8431)
                     /locus_tag="OO339_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5925823.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="OO339_001114"
     CDS             complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="OO339_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5925824.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9789..10436
                     /gene="acrR"
                     /locus_tag="OO339_001115"
     CDS             9789..10436
                     /gene="acrR"
                     /locus_tag="OO339_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5925825.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10564..13926
                     /gene="mscK"
                     /locus_tag="OO339_001116"
     CDS             10564..13926
                     /gene="mscK"
                     /locus_tag="OO339_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5925826.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="OO339_001117"
     CDS             complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="OO339_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5925827.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14313..14840)
                     /gene="priC"
                     /locus_tag="OO339_001118"
     CDS             complement(14313..14840)
                     /gene="priC"
                     /locus_tag="OO339_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5925828.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14910..15287
                     /locus_tag="OO339_001119"
     CDS             14910..15287
                     /locus_tag="OO339_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5925829.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15440..15991
                     /gene="apt"
                     /locus_tag="OO339_001120"
     CDS             15440..15991
                     /gene="apt"
                     /locus_tag="OO339_001120"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5925830.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16120..18045
                     /gene="dnaX"
                     /locus_tag="OO339_001121"
     CDS             16120..18045
                     /gene="dnaX"
                     /locus_tag="OO339_001121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5925831.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17378..17442
                     /gene="dnaX"
                     /locus_tag="OO339_001121"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18098..18427
                     /locus_tag="OO339_001122"
     CDS             18098..18427
                     /locus_tag="OO339_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5925832.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18427..19032
                     /gene="recR"
                     /locus_tag="OO339_001123"
     CDS             18427..19032
                     /gene="recR"
                     /locus_tag="OO339_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5925833.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19142..21016
                     /gene="htpG"
                     /locus_tag="OO339_001124"
     CDS             19142..21016
                     /gene="htpG"
                     /locus_tag="OO339_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5925834.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21197..21841
                     /gene="adk"
                     /locus_tag="OO339_001125"
     CDS             21197..21841
                     /gene="adk"
                     /locus_tag="OO339_001125"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5925835.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21973..22935
                     /gene="hemH"
                     /locus_tag="OO339_001126"
     CDS             21973..22935
                     /gene="hemH"
                     /locus_tag="OO339_001126"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5925836.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22932..23891)
                     /gene="aes"
                     /locus_tag="OO339_001127"
                     /pseudo
     CDS             complement(22932..23891)
                     /gene="aes"
                     /locus_tag="OO339_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24043..25347
                     /gene="gsk"
                     /locus_tag="OO339_001128"
     CDS             24043..25347
                     /gene="gsk"
                     /locus_tag="OO339_001128"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5925837.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25480..27156)
                     /locus_tag="OO339_001129"
     CDS             complement(25480..27156)
                     /locus_tag="OO339_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5925838.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLKTPPAGEVVTG"
     gene            complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="OO339_001130"
     CDS             complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="OO339_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5925839.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28832..30484
                     /gene="ushA"
                     /locus_tag="OO339_001131"
     CDS             28832..30484
                     /gene="ushA"
                     /locus_tag="OO339_001131"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5925840.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="OO339_001132"
     CDS             complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="OO339_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5925841.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31204..31998)
                     /locus_tag="OO339_001133"
     CDS             complement(31204..31998)
                     /locus_tag="OO339_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5925842.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32136..32477
                     /locus_tag="OO339_001134"
     CDS             32136..32477
                     /locus_tag="OO339_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5925843.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32692..35196)
                     /gene="copA"
                     /locus_tag="OO339_001135"
     CDS             complement(32692..35196)
                     /gene="copA"
                     /locus_tag="OO339_001135"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5925844.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35458..36390
                     /gene="glsA"
                     /locus_tag="OO339_001136"
     CDS             35458..36390
                     /gene="glsA"
                     /locus_tag="OO339_001136"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5925845.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36393..37685
                     /locus_tag="OO339_001137"
     CDS             36393..37685
                     /locus_tag="OO339_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5925846.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37810..38217
                     /gene="cueR"
                     /locus_tag="OO339_001138"
     CDS             37810..38217
                     /gene="cueR"
                     /locus_tag="OO339_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5925847.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38218..38676)
                     /locus_tag="OO339_001139"
     CDS             complement(38218..38676)
                     /locus_tag="OO339_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5925848.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38673..39590)
                     /locus_tag="OO339_001140"
     CDS             complement(38673..39590)
                     /locus_tag="OO339_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5925849.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39736..40413
                     /gene="fetA"
                     /locus_tag="OO339_001141"
     CDS             39736..40413
                     /gene="fetA"
                     /locus_tag="OO339_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5925850.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40400..41179
                     /gene="fetB"
                     /locus_tag="OO339_001142"
     CDS             40400..41179
                     /gene="fetB"
                     /locus_tag="OO339_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5925851.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="OO339_001143"
     CDS             complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="OO339_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5925852.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="OO339_001144"
     CDS             complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="OO339_001144"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5925853.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="OO339_001145"
                     /pseudo
     CDS             complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="OO339_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43549..44235
                     /locus_tag="OO339_001146"
     CDS             43549..44235
                     /locus_tag="OO339_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5925854.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44232..46647
                     /locus_tag="OO339_001147"
                     /pseudo
     CDS             44232..46647
                     /locus_tag="OO339_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OO339_001148"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OO339_001148"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5925855.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OO339_001149"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OO339_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5925856.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="OO339_001150"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="OO339_001150"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5925857.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="OO339_001151"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="OO339_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5925858.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="OO339_001152"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="OO339_001152"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5925859.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53217
                     /locus_tag="OO339_001153"
     CDS             52938..>53217
                     /locus_tag="OO339_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5925860.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRW"
BASE COUNT        12829 a        13689 c        14019 g        12680 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc ccttcgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaggcc gcttacgcgg ccttagtgat tacatgttgt
     5281 atcaatgatg atcgacagta tggctgtgct cgatatcttc attcttgcgg ctaaagcggc
     5341 ggcgaaccac cacaaagaat accggaacga agaagattgc cagtaccgtt gcggtcacca
     5401 tcccgcccat tacaccggta cctactgcgt tctgcgcgcc ggaaccagca ccagtactga
     5461 taaccagcgg cataacgccg aggataaacg ccagcgaggt catcaggatc ggacgtaaac
     5521 gcatccgcac cgcatcaagc gtcgcttcaa tcagaccttt accttcttta tccatcaagt
     5581 ctttggcgaa ttcgacgata agtatcgcgt tcttcgccga caacccaatg gttgtgagca
     5641 gtcctacttg gaagtaaacg tcattggtca ggccacggaa ggtggcagcc agcaacgcac
     5701 cgataacccc cagcggaacg accagcataa cggagaacgg agtcgaccag ctctcgtaca
     5761 gcgccgccag acacaggaat acgacaatca acgaaatcgc gtacagtgaa ggtgcctggt
     5821 tgccggagag acgttcctga taggacatcc ccgtccagtc atagccaaca ccggtaggca
     5881 gtttgctcgc cagttgttcc atcagctcca ttgcttcacc ggtactttta cccggtgccg
     5941 cctggcctaa gatttccatg gatggcaggc cgttgtaacg ttccagacgc ggcgaaccgt
     6001 actcccaacg agaagaggag aacgccgaga atggcaccat ctgaccatca gcagcacgaa
     6061 cataccagtc gccgatatca tccggcagca tacggtattt cgcttctgac atgacgtaaa
     6121 ctttcttcac acgaccgcgg tcgataaagt cgttcacata gctaccgccc catgcagcgc
     6181 ccagagtggt gttaatgtcg ttgatagaaa cacccagcgc ctgcgctttt tcctggtcga
     6241 tatcaatctt aaactgcggg gtatcttcca gaccgtttgg acgtacgctg gtcagcatat
     6301 cagggtgctt cgctgcttct gcaagcagct ggttacgcgc ctgagtcagt ttttcgtgac
     6361 caaggccagc ctggtcaatc agctcaaagt caaagccggt tgcagtaccc agttccacga
     6421 ttgcgggcag gttaaaggcg aaaaccatcg catctttgat ttgcgagaaa gcgcgtgttg
     6481 cacgcatggt aatcgcttca actttgtttt cttcgcccgg acgatcggcc cagtccttca
     6541 aggaaacgaa cgcaataccg gtattctgac cacgtcccgc aaagccgaag ccgttaacgg
     6601 cgaacaccga ctcaacgttg ttcttttctt tggtcagata gtaatgcgtt acctcattga
     6661 gcactttctg cgtacgttcc tgcgttgcac ctgctggcag ctgaaccatg gtcataaata
     6721 cgccctggtc ctcatctggc aagaaggagc ttggcagacg cacgaacaga taggccatgc
     6781 cgaccacgat gatcagatac agcactaggt aacgccccgt actgcgcaga ataccgccta
     6841 cgctgtcggt gtagtggtgc gtgctcttct cgaacatgcg gttcaaccag ccgaagaagc
     6901 cttttttacc ttccccgtga tcgcctttgg caatcggttt cagcatggtg gcacaaagag
     6961 ccggagtcag gatcaacgct accagtaccg acagcgccat tgctgaaaca atggtaatag
     7021 agaactgacg atagatagca ccagtagaac cgccaaagaa ggccatcggt acgaataccg
     7081 ccgacagtac catcgcgata ccgaccagag cgccctgaat ctgccccatc gacttacggg
     7141 tagcttcttt tggcggcaaa ccttcttccg ccataacacg ctccacgttt tctaccacaa
     7201 cgatagcgtc atccaccaac aagccgatgg cgagcaccat cccgaacatt gttagcgtgt
     7261 ttatcgagaa gccaaaggcg gcaaggacgg caaaggtccc gagcaatacc accggtacgg
     7321 caatggtcgg aatcaacgtc gcgcggaagt tctgcaggaa cagatacata accaggaaca
     7381 cgaggatgat cgcttcgacc agcgttttaa ccacttcgtg aatagagatt ttcacgaacg
     7441 gcgtggtgtc gtacgggtaa acaattttca gacccgacgg gaagaacggt tccatcttcg
     7501 ccagttcagc acggattgcc gcagcggtat ccagcgcgtt tgcaccggtc gccagcttga
     7561 tccccagacc ggaagcaggt tggccgttaa actctgcgat gatgtcgtag ttctcaccac
     7621 ccagctcaat cttcgccaca tcacgcagca gtacgcggga accatcctga ttcactttca
     7681 gcaggatttt gccgaactct tcagtagagg tcagacgcgt ctgagcaata atagaggcgt
     7741 taagctgttg gcctttcacc ggcggcgtac caccgagctg acccgccgca acctgggcgt
     7801 tctgcgcttt gatggcggta atgacatcaa ccggcgttag ctggaatttg ttcagctcat
     7861 tcgggttcat ccagatacgc atcgcgtact gtgaaccgaa caactgaaca tcacccacgc
     7921 ccgacgtacg gctgatggca tctttcatat tcgccgctac gtagtcagag atatcctcct
     7981 gcgtcatggt gccatcggtg ttgataacgc cgacaaccat caggaagctg ctggatgatt
     8041 tctcaacgct caccccttgc tgctgaactt cttgcggcag caacggcatc gccagctgca
     8101 gtttgttctg cacctgaacc tgcgcgatat ccgcatcagt accagactca aaggtcaggg
     8161 tgatctgcac ggtacccgtg gagtcactgt tagaggacat gtacatcagg ttatcgatac
     8221 cgttcatatt ctgttcgata acctgtgtca ccgtgtcctg cactgttttc gcatcagcgc
     8281 cggggtagga ggcggagatc gttactgccg gcggtgcaat cgtaggatat tgcgccaccg
     8341 gcagtttgag gatcgccagc ccccctgcca acatgatgat aatggcgatc acccacgcaa
     8401 aaatcgggcg atcgataaag aaattaggca tgtcttaacg gctcctgttt aagttaagac
     8461 ttggactgtt caggctgagc accgcttgcg gcttgctggt tattatcagc ggtaacttct
     8521 tgtgctttta cctggacacc aggacgcact ttctgcagcc cacttattac tacgcgatcg
     8581 cctgctttca gaccttctgt caccagccac ttatcgccaa tagcctggct tgcaacgatc
     8641 ggacgggttt ccactttgtc atccgcgcca actaccagta cggtggcatc accacgcggc
     8701 gtacgggtta cgccctgttg cgggactaaa atagcgtttg gattaagccc ttcttccaga
     8761 cgtgcgcgca cgaacatacc cggcagcaga gtgtgatccg ggttcgggaa gatagcgcgt
     8821 agggtgatag acccagtggt ctgatcaacg gtaacgtcag agaattccag cgtaccgtcc
     8881 tgcgggaact taatgccgtc actggtgatc agcgacactt tggctttgcc gttctcttgt
     8941 ttcagcgtgc cattcgccag ttcctgtttc aggcgaagga agtcgttgct ggactgggtc
     9001 acatcaacgt agatcggatc aagttgctgc acggttgcca gcacagtcgc ctgaccgttc
     9061 tgtaccaatg cgccttccgt cacgttcgac ttaccaatgc gaccgctaat cggagaggtg
     9121 actttggtgt aagccagatt gatccgcgca gtttcaacgg cagctttcgc cgcagttacc
     9181 gcagcattcg cctgttgcgc atcagccaga gcctgatcgt acccttgctt actgatgtac
     9241 tgagtaccga gcagtttctg ataacgattc accgtcaatt gcgcgatatt ggctgcagcc
     9301 tgggctttcg ccagatcacc tttcgcactg tcgtatgtcg cctgataggt cgcaggatca
     9361 atctgataga gagagacacc agcttcgatg tcgctacctt ctttgaaatt acgcttcagg
     9421 ataatcccgc taacttgagg acgaacttct gcgatccggt aggcactggt gcgacctgga
     9481 agctcggttg tgatctgcag aggttcagtt ttgactgtta ctacgccaac ggcgggcatc
     9541 tgctggccac cttgttgggc ctgtttgtcg tcacatcctg ttagggctaa gctgcctgag
     9601 agcatcagaa cgaccgccag aggcgtaaac cctctgtttt tgttcatatg taaacctcga
     9661 gtgtccgatt tcaaattggt caatggtcaa aagttaataa acccattgct gcgtttatat
     9721 tatcgtcgtg ctatggtaca tacattcaca aatgtatgta aatctaacgc ctgtaaattc
     9781 acgaacatat ggcacgaaaa accaaacaag aagcgcaaga aacgcgccaa cacatcctcg
     9841 atgtggctct acgtcttttc tcacagcagg gggtatcatc cacctcgctg ggcgagattg
     9901 caaaagcagc tggcgttacg cgcggtgcaa tctactggca ttttaaagac aagtcggatt
     9961 tgttcagtga gatctgggaa ctgtcagaat ccaatattgg tgaactagag cttgagtatc
    10021 aggcaaaatt ccctggcgat ccactctcag tattaagaga gatattaatt catgttcttg
    10081 aatccacggt gacagaagaa cgacgtcgat tattgatgga gattatattc cacaaatgcg
    10141 aatttgtcgg agaaatggct gttgtgcaac aggcacaacg taatctctgt ctggaaagtt
    10201 atgaccgtat agaacaaacg ttaaaacatt gtattgaagc gaaaatgttg cctgcggatt
    10261 taatgacgcg tcgggcagca attattatgc gcggctatat ttccggcctg atggaaaact
    10321 ggctctttgc cccgcaatct tttgatctta aaaaagaagc ccgcgattac gtcgccatct
    10381 tactggagat gtatctcctg tgccccacgc ttcgtaatcc tgccactaac gaataaccct
    10441 gaatctgact ccaggatttt tcctggacat tttcgtcgtt gctattctgg ttcactgcgt
    10501 cgtgatattc ttgcggtttg actttttcag gtcgttcttc aggttcagaa accttcattc
    10561 atcatgacta tgttccagta ttacaaacga tcacggcatt ttgttttttc agcatttatt
    10621 gcttttgttt ttgtcttgtt atgccagaac acggcgtttg cgcgggcgtc atcgaatggt
    10681 gatctgccga caaaagcgga cctgcaggcg caacttgact cactaaataa acaaaaagat
    10741 ctttctgctc aggacaaact ggtgcagcag gatctgacag atacattagc caccctcgat
    10801 aaaatcgatc gcataaaaga agagacagtt cagctacggc aaaaagtcgc tgaagcgccg
    10861 gaaaaaatgc gccaggcgac cgcggcgtta acagcactta gcgatgtcga taacgacgaa
    10921 gaaacgcgca aaattctgag tacgctttcg ttgcgccagc tggaaactcg cgttgcccag
    10981 gcgctggacg atttgcaaaa cgcacaaaac gatctggcgt cttataacag ccagctggtt
    11041 tcgttacaga cgcagcccga acgcgtgcaa aatgcgatgt ataacgcttc gcagcagctg
    11101 caacaaattc gcagtcgtct ggatgggact gatgtcggcg agactgcctt acgtcccagc
    11161 cagaaagtgt taatgcaggc tcagcaggcg ttgctgaatg cggagattga ccagcagcgt
    11221 aaaagcctgg aagggaacac cgtcttgcag gataccttgc aaaagcaacg tgattacgtg
    11281 acggcgaaca gcgcccgtct ggagcaccag ttgcagctgt tgcaagaagc ggtaaacagc
    11341 aagcgcctga ctttaactga aaaaacggcg caggaagccg tctccccgga tgaagccgcg
    11401 cgtattcagg ctaatccgct ggtgaaacag gaactggaaa ttaaccagca gttaagtcag
    11461 cgtctgatta ccgcgactga aaacggtaat cagttgatgc agcaaaacat taaagtcaaa
    11521 aactggctgg agcgggcgct gcaatcggaa cgcaatatta aagagcagat tgccgtcctg
    11581 aagggcagcc tgctgttgtc tcgtatcctt taccagcaac aacaaacgct gccctcggcg
    11641 gatgaactgg aaaacatgac caaccgcatc gcggatttgc gtctcgaaca gtttgaagtt
    11701 aaccagcagc gtgatgcact cttccagagc gatgcgttcg tcaacaaact ggaagaaggt
    11761 cacaccaacg aagtcaacag cgaagttcac gatgcgttat tgcaagtggt tgatatgcgt
    11821 cgcgaattgc tggatcaact caacaaacag ttgggtaacc agctgatgat ggccattaac
    11881 ctgcaaatca accagcagca gttaatgagc gtatcgaaaa acctgaaatc catcctgact
    11941 cagcaaatct tttgggtgaa cagtaaccgt ccaatggact gggactggat caaagcgttc
    12001 ccgcaaagcc tgaaagatga atttaagtcg atgaaaatca cggtgaactg ggaaaaagcc
    12061 tggcctgccg tttttatcgc tttcctcgcc ggtttgccgc tgctgttgat tgccgggctg
    12121 atccactggc gtctgggctg gctgaaagcg tatcaacaga aactggcttc cgctgtgggt
    12181 tccctgcgta acgatagcca gctcaacaca ccaaaagcga tccttatcga cctgatccgc
    12241 gcgctgccgg tgtgcctgat tattctcgcg gttggcctga ttctgttgac catgcagctc
    12301 aacatcagcg aactgctatg gtcgttcagt aaaaaactgg cgattttctg gctggtgttt
    12361 ggcctgtgct ggaaggtact ggagaaaaat ggcgttgccg tgcgtcactt cggcatgccg
    12421 gagcagcaga ccagccactg gcgtcggcaa attgtccgca tcagtctcgc gttgctgcct
    12481 atccatttct ggtctgtggt ggcagaactt tccccgctgc atctgatgga tgatgtgctg
    12541 gggcaggcga tgattttctt caacctgctg ctgattgcct tcctggtatg gccaatgtgc
    12601 cgcgaaagct ggcgtgataa agagtcgcac accatgcgac tagtcaccat taccgtgctg
    12661 tcgattatcc cgattgcatt gatggtgctg actgccacgg gttatttcta caccacgctg
    12721 cgcctctccg ggcgttggat tgaaaccgtt tatctggtga tcatctggaa cttgctctac
    12781 cagacggtat tgcgcggctt aagcgtagcg gcgcggcgta tcgcctggcg tcgtgcgctg
    12841 gcgcgtcggc agaatctggt gaaagagggc gcagaaggtg ccgaaccgcc ggaagaaccc
    12901 accattgcac tggagcaagt taaccagcag acgctgcgta ttaccatgtt gctgatgttt
    12961 gcgctgttcg gtgtcatgtt ctgggcaatt tggtccgatt tgatcaccgt gttcagctat
    13021 ctcgacagca tcacgctctg gcattacaac ggcactgaag ctggcgctgc ggtggtgaaa
    13081 aacgtcacca tgggcagtct gttgtttgcg attatcgcct caatggtggc ctgggcgttg
    13141 attcgcaacc tgcctggttt actggaagtg ctggtgctct cgcgactgaa tatgcgccag
    13201 ggcgcgtcgt atgccattac taccatcctt aactacatca ttattgccgt tggtgcgatg
    13261 acggtgttcg gatcgctggg cgtctcttgg gataaactcc agtggctggc cgcagcatta
    13321 tccgtaggtc ttggttttgg tttacaagaa attttcggta acttcgtctc cggtttgatc
    13381 attctgttcg aacgtccggt gcgtattggc gatacggtaa ccattggtag cttctcgggg
    13441 acggtaagta agatccgtat tcgtgcgaca acgattaccg atttcgatcg caaagaagtg
    13501 atcatcccga acaaagcgtt tgttaccgag cgtctgatca actggtcgtt gactgacact
    13561 actacgcgtc tggtgatccg tctcggcgtg gcctatggct ccgatctgga aaaagtgcgt
    13621 aaagtgttac tgaaggcggc gactgagcac ccaagggtga tgcacgaacc aatgccggaa
    13681 gtcttcttta cggcatttgg tgccagcacg ttggatcatg agctgcgtct gtatgtgcgt
    13741 gaactgcgtg accgtagccg tactgtcgat gagctgaacc gtactatcga tcagctgtgc
    13801 cgtgaaaacg acatcaacat tgcctttaac cagcttgaag tgcatctgca caacgagaag
    13861 ggcgatgagg tgacggaagt aaaacgcgac tacaaaggcg acgatccgac accagcggtt
    13921 gggtaataac caaagggggc gacatttagt tgccccgaga ttactgacaa agtgcgcgtt
    13981 gttcatgccg gatgcggcgt gaccgcctta tccggcctac gaaaccgcaa gaattcaata
    14041 tattgcagga gcggtgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgccc
    14101 gcaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgt
    14161 gcgttaattt cttttcataa tcacgcttta caatatccag cgcgcgcagc acggtactgg
    14221 cagggatctg attttcctcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
    14281 gggcattttc aagtgacata ctctttccat tgctagcggg ttaagcgcgc tagcctgttt
    14341 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
    14401 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
    14461 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
    14521 cgggaaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
    14581 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
    14641 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcgtc gagacaagac
    14701 tgtagtgttg tcgcacgagt ctgaaaaaga tgcctgtcga aacgagcact tagcgtggcg
    14761 aactgtgaca ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
    14821 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
    14881 agccactatg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
    14941 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
    15001 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
    15061 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
    15121 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
    15181 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
    15241 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
    15301 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
    15361 gtcaaaggtt aaacaacagt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
    15421 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
    15481 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
    15541 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
    15601 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
    15661 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
    15721 aaccatcagt gaaaattacg acctggaata cggcaccgat cagctggaaa tccacgttga
    15781 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
    15841 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
    15901 cattatcaac ctgtttgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
    15961 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
    16021 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
    16081 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggttttagcc
    16141 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
    16201 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggcacccgt
    16261 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
    16321 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
    16381 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
    16441 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
    16501 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
    16561 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
    16621 tcacgctgtc tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcagctt
    16681 gagcacatcc tcaatgaaga acatatcgct cacgagccgc gggcgctgca attgttggcg
    16741 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
    16801 ggtgacggcc aggtttcaac ccaggcggtc agtgcgatgc tgggtacgct tgacgacgat
    16861 caggcgctgt cgctggttga agcgatggtc gagggcaacg gcgagcgcgt aatggcactg
    16921 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
    16981 ttgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
    17041 gccatcgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
    17101 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
    17161 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cgcgtatgcc gctgcctgag
    17221 ccagaagtgc cacgccagtc ctttgcaccc gtcgcgccaa cggcagtaat gacgccaacc
    17281 ctggtgccgc cgcaatcagc gccgcagcag gcaccgactg taccgctccc ggaaaccacc
    17341 agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa
    17401 aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc gctggaaaga
    17461 ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg
    17521 ccagccaaaa aagaagcgta tcgctggaag gcgaccactc cggtgatgca gcaaaaagaa
    17581 gtggtcgcca cgccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg
    17641 gcggtgaagc tagcggcaga agccattgag cgcgacccgt gggcggcaca ggtgagccaa
    17701 ctttcgctac caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac
    17761 aacgcagtat gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggtgcacag
    17821 caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa cggttgaact gactatcgtt
    17881 gaagatgata atcccgcggt gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa
    17941 aaacttgcgc aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcgg
    18001 tttttcgatg cggagctgga tgaagaaagt atccgcccca tttgatcgta agcacagctt
    18061 acgttcgtca tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg
    18121 taacctgatg aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat
    18181 cgcgcagctg gaagttaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg
    18241 tgcacacaac tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat
    18301 gctggaagac ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca
    18361 gaaagaaaaa atggcctctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc
    18421 gttctgatgc aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgtctgccg
    18481 ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc
    18541 ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc
    18601 gattgccgca ctttcaccga acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag
    18661 gaaaacggtc aaatctgtgt ggtggagagt ccggcggaca tctacgccat tgagcagacg
    18721 gggcagtttt ctggtcgtta ttttgtgttg atggggcatc tgtcaccgct ggacggcatc
    18781 ggtccggatg atatcgggct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact
    18841 gaagtgatcc tcgccaccaa tcccacggtt gaaggtgaag ctaccgctaa ctacattgcc
    18901 gagctttgcg cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggc
    18961 ggcgaactgg aaatggtcga tggcaccacg ctgtcacact ccctcgccgg gcgtcataag
    19021 attcgttttt aagcaaacga gagcaggagc acctgctctc gcttgaaatt attctccctt
    19081 gtccccatct ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta
    19141 catgaaagga caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat
    19201 gatccattct ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc
    19261 cgatgcggcg gacaagctgc gtttccgtgc gctatctaac ccggacctgt acgaaggtga
    19321 tggcgaactg cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga
    19381 taacggcgtg gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc
    19441 cggtaccaaa tcattcctcg aatccctggg ttctgaccaa gcgaaagaca gccagctgat
    19501 cggtcagttt ggtgttggtt tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg
    19561 tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg
    19621 tgaatatacc gttgccgata tcaccaaaga agatcgtggt actgaaatca ccctgcatct
    19681 gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg cgttccatca tcagcaaata
    19741 ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag atggcgaaac
    19801 cgttatctcc tgggcaaaaa tcaacaaagc gcaggcgctg tggactcgta acaagtcgga
    19861 aatcaccgat gaagagtata aagagttcta caaacacatc gcccacgact ttaacgatcc
    19921 gctgacctgg agccacaacc gcgttgaagg taagcaggag tacaccagcc tgctgtacat
    19981 cccgtcccag gctccgtggg atatgtggaa ccgtgatcat aaacacggcc tgaaactgta
    20041 cgttcaacgc gtgtttatca tggacgacgc tgaacagttc atgccgaact atctgcgctt
    20101 cgtgcgtggt ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca
    20161 ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct
    20221 ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct
    20281 ggtactgaaa gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaactgct
    20341 gcgttttgct tctacttata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt
    20401 ttcccgcatg aaagaagggc aggagaaaat ctactacatc actgcagaca gctatgcggc
    20461 agcgaagagc agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct
    20521 ttccgaccgc atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt
    20581 ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag
    20641 cgcgaaagaa gcggagaaag cactgactcc gttcatcgac cgcgtgaaag ctctgctcgg
    20701 cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc gatacgccag cgatcgtttc
    20761 gaccgacgcg gacgaaatga gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa
    20821 agtgccagaa gtgaaataca tcttcgaact gaacccggat cacgtactgg tgaaacgtgc
    20881 ggcagatact gaagatgaag ccaagttcag cgagtgggta gaactgctgc tggatcaggc
    20941 gctgctggca gaacgcggca cgctggaaga tccgaacctg tttattcgtc gtatgaatca
    21001 gctgctggtt tcctgatgtc atgccggatg acctttgtgt catccggcat ttttcttttc
    21061 atcatctgta ctttccgcaa attatctcgc cattaaccgt ttcagcctca ggtgcctttc
    21121 ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt tcaaaaaaat cgacacattt
    21181 taaggggatt ttcgcaatgc gtatcattct gcttggcgct ccgggcgcgg ggaaagggac
    21241 tcaggctcag ttcatcatgg agaaatatgg tattccgcaa atctccactg gcgatatgct
    21301 gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa gcaaaagaca ttatggatgc
    21361 tggcaaactg gtcaccgacg aactggtgat cgcgctggtt aaagagcgca ttgctcagga
    21421 agactgccgt aatggtttcc tgttggacgg cttcccgcgt accattccgc aggcagacgc
    21481 gatgaaagaa gcgggcatca atgttgatta cgttctggaa ttcgacgtac cggacgaact
    21541 gattgttgat cgtatcgtag gccgccgcgt tcatgcgccg tctggtcgtg tttatcacgt
    21601 taaattcaat ccgccgaaag tagaaggcaa agacgacgtt accggtgaag aactgactac
    21661 ccgtaaagac gatcaggaag aaaccgtgcg taaacgtctg gttgaatacc atcagatgac
    21721 tgcaccgctg atcggctact actccaaaga agcggaagcg ggtaacacca aatacgcgaa
    21781 agttgacggt accaagcagg ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta
    21841 atcaagccgc agcagatctg ctttcgggca ggtctgctta tccacctcag gcaatacctc
    21901 tcacagcaat tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga
    21961 taagaggcgg taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc
    22021 gatgccccca cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc
    22081 gtggttgata cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg
    22141 cgctcgccgc gtgtggcgaa gctgtacgcc tctgtctgga tggaagacgg ctcgccgctg
    22201 atggtttaca gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgccagtg
    22261 gcgctgggca tgagctacgg ctcgccatca ctggaaagct ccgtcgatga actcctggca
    22321 gagcatgtag atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc
    22381 ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata
    22441 tcgtttattc gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta
    22501 cgcgcttctt ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt
    22561 ccccagcgtt atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc
    22621 gaactggctt ccgcactggg gatggtaccg gaaaaagtga tgatgacctt tcagtcgcgc
    22681 tttggccggg aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa
    22741 aaaggcgtag gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg
    22801 ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg gtgcgggcgg gaaaaaatat
    22861 gaatatattc cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt
    22921 gccgcgtatc gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg
    22981 gcggttttca tcatccgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg
    23041 aactcacagg gctgctgatg cgccgctaac gtctagtaaa gcagacggct gtcatccagc
    23101 agcggatcga actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta
    23161 ttaaacagac agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca
    23221 tacatctgca aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc
    23281 acggaatccc gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa
    23341 tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa
    23401 tcaccggcaa agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa
    23461 taacaacaag cagccacaat ttcctctatc gcttgtggaa aacgcgcttc aggtgaaagg
    23521 gtgtaattaa taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga
    23581 tcgtgggtat cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc
    23641 gggctatctg gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc
    23701 atgtaagctc tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg
    23761 taatactgtc gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga
    23821 agagtattca caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt
    23881 tccggcttca tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa
    23941 aatgcgatcc cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg
    24001 tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa
    24061 cgtaaatcca aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag
    24121 ccagaaaacg aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt
    24181 gaagcgaaag tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg
    24241 gtgattgagg atgacgtagc cgaagtgctt tatcaggagc taaaacagaa aaacctgatt
    24301 acccatcagt ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg
    24361 gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc
    24421 tatcgttacc tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat
    24481 ggtccgattg gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc
    24541 agtccaggcc acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga
    24601 gcctcggcac tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg
    24661 gaagcaacca tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg
    24721 ctgggcacca agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac
    24781 cacgtttcta tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat
    24841 ccgttgttgg catctgacaa ggcgctggac tgggtagagc tggtgctgtg caccgccggg
    24901 ccaatcggct tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat
    24961 ccgctgctgc cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc
    25021 cacaaggatt gccagaatcc gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg
    25081 ccggaaaaaa tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat
    25141 gacattaccg ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc
    25201 acctggttaa cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat
    25261 caggtactga accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc
    25321 caggaagagt cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt
    25381 cacgccgcat ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg
    25441 cctacatatt tttcatattt tacatccggc aaccaccgtt taccccgtca ccacttcacc
    25501 cgccggtggc gttttcagca gttccagcat agtacgggca atttcacgtt cacccatcac
    25561 tacctgattc gcgccacgtt cggtgatata cgtcacttca tcgtcataat gggcgcgggc
    25621 gataatctca atatccgggt ttttcgcgcg ggcagacgcc acaatctcac ccgcttcata
    25681 accgttgggg atcgtcagga tcagccattt tgcacattcc agatgtgcca gttgcataat
    25741 ttcttcgttc gctgcattgc ccaatacagc gcggacccca cgctctcgca gctcatcaac
    25801 acgggttcgt gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc
    25861 cagcaggctg cctacacgac cgtaacccac caatagcgca tggttgcaaa tatccactgg
    25921 gatctgtttc tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt
    25981 cgccagatat ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc
    26041 ccctgccagt accaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc
    26101 cgccaggata aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt
    26161 acgttgggag tgaccaaaca gacggacaag gaacagggcc gcgaccgatt taccaaacag
    26221 aataattgcc agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa
    26281 caacatcccg actgagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcaacgtatc
    26341 gtgggcggca cggtgactca attcagactc gttcagtacc atcccggcaa agaacgcacc
    26401 gagtgcaaag gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc
    26461 cagcactgac agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca
    26521 cggcaccaga cggcgaccta ccagcatcat aatggcgata aatgcgatca ccttgccgat
    26581 ggtgatcccc atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc
    26641 tgccactgcg ggcagcaaca ccagcgtcag aaccatcacc aggtcttcca caatcaacca
    26701 accgatggcg atttgcccac gctgactgtc tattaattgc cgttcttcaa gtgcgcgcag
    26761 taacaccacg gtactggcgg tggaaagaca taagccgaac acgataccgg tcattaacga
    26821 ccagcccagc acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat
    26881 cgcgccggga atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc
    26941 gacgccgaac atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc
    27001 ggcaacaaag cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag
    27061 aggagaaata cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc
    27121 aacaatggtg gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta
    27181 ttgtccattt ttggctggga aaaccaaaat tacaggtaat agtttatgac aatttcattg
    27241 atgatgttta tgaataattg ttgaattttg cagaaaaatg gaattagctg caaaaaaagc
    27301 acggttcgga aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga
    27361 tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag
    27421 gaatatggtc aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat
    27481 gctggtgtga tcggcgataa gccccagaac tgccgctccc agacctccca tgccgaaagc
    27541 aaaaccgaaa aagagtccag aaaccatacc gatacgtccc ggaagtagct cctgagcgta
    27601 gaccagaatg gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc
    27661 cgtccagtgc aggcgggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga
    27721 gccccaaatc acatatttcc gtccaatttt atcccctaca ggcccgccga tcaccgtacc
    27781 tgccgcaacg gcaaacagga aggcaaacag atgaagctgg gcattctgga tagataatcc
    27841 gaatttttgc atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt
    27901 cgagaaaatg aggattaaca gaatgctgac tgccagtacc actttgttgc gcggcagtgg
    27961 attgattatc gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg
    28021 gctaatttgc gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt
    28081 gcctttgcca taaggctcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa
    28141 gttgccgccg acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg
    28201 ggctacgcga gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgctgc
    28261 cagcagaact gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa
    28321 gcacatgcca attggcaacg accacggcat cggatattta tcggtccagt agccgaccac
    28381 tggttgcagt agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa
    28441 tgtcagagaa aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat
    28501 gtcgttcagc agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt
    28561 ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt tcactcattg ccataggaaa
    28621 gtcacttttt cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa
    28681 catacctgta tgcgtcgtct gaaggaagtc tcaacgccga atacagaatt tctaatctgg
    28741 atgcagattt atcttcaccg gacgcagact tgtctatgat gttgcgtcat actatttttc
    28801 aacacgttga aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt
    28861 agcgctgtta accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa
    28921 aacctacaaa attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga
    28981 atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt
    29041 tgcggctgaa ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc
    29101 cgagtctgac ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga
    29161 cgcgatggcg atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga
    29221 aaagtgggcc aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg
    29281 cctgtttaaa ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg ttattgggct
    29341 gacaaccgat gacacagcaa aaattggtaa cctggaatac ttcactgata tcgaatttcg
    29401 taagcccgcc gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga
    29461 cattattatc gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc
    29521 accgggcgat gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg
    29581 tggtcactcg caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt
    29641 gccgggtacg ccatgcaaac cagatcaaca aaacggcatc tggattgtgc aggcgcatga
    29701 gtggggcaaa tacgtgggac gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt
    29761 taactaccag ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag
    29821 cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc
    29881 accgttccag aacaaaggca aagcgcagct ggaagtgaaa ataggcaaaa ccaatggtcg
    29941 tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct
    30001 ggcagcccaa atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg
    30061 tgattctatc gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg
    30121 caatgtggtg gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt
    30181 cgcgcagatg aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc
    30241 gaaagacggc aaactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac
    30301 ttaccgtatg gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga
    30361 taacaaaccg ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat
    30421 ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca
    30481 gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat
    30541 cagcaaattt ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc
    30601 cgcgcttgcc gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg
    30661 ttggcagacg ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac
    30721 gctgcgcgac catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt
    30781 ttttaagatc cagttgacct gcgaccggcg taacggccac ggcaaggtgt ttcatatcac
    30841 cgttcactgc caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga
    30901 cgacttcatc gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg
    30961 aaatcttgtt tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa
    31021 caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta
    31081 agagtattgg caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga
    31141 cggcataata ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt
    31201 tttttagcgc aacatttgcg gcaaattccc ttctccatac aggtgtagtg caccgaccgc
    31261 gaccacatat cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg
    31321 atgcatcagc acatcgtaca gcgactgact gaacgtattg ggcagcgtta tatcattatt
    31381 ttgcggcggt gcattcagcc accagctcat catttgttgc agcaaccgtg cgttggtatg
    31441 ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat
    31501 ggcaatctgg ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc
    31561 ctgcaatagc tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt
    31621 cgcctgaaga accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat
    31681 gcccatctcc tggctaatgt gctgcaggtt ttggagttgc tcctcgctaa tgcgctcttc
    31741 cagcgcctcg caggcaggca aattagcaaa aggcgtatcg ctggtggaaa catccgcctc
    31801 gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat
    31861 atcgtggcta cccatatgaa tactgccaat cagatgaaaa tggcgattgc cggggagggt
    31921 gatatcgatg gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac
    31981 ccggtacaac agatccatac gacctccctt tgtgaaatat catgctagcg cgcggtgagg
    32041 gatggcgcaa atggagttgg ggacttgttt aatgtgtttg tatgattcag tatgttcttg
    32101 catcgctatt cacagggaag caacagttaa aaaccatgaa acaggcaaca agaaaaccga
    32161 cgacacccgg agatattctt ctctatgaat atctggaacc gctcgatttg aaaatcaatg
    32221 agttagcaga gttgctgcat gttcatcgta atcgcgtcag tgcactgatc aataacaatc
    32281 gtaaactcac cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt
    32341 ttaggctaaa cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc
    32401 aggaagaatt gggacggatt gaaacagtgg ctgaatattt ggcacgccgt gaagagcgtg
    32461 caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttca
    32521 cgccgcatcc ggcatccgag caccattgcc tgatgcgacg cttacgcgtc ttatcaggcc
    32581 tacaaacctg tgcctgaacc gtaggccgga taaggcgttt acgccgcatc cggcagtcat
    32641 gcgtcgatgc caaatgcgcc gccctaaagc agcgcatccg caatgacgta cttattcctt
    32701 cggtttaaac cgcagcaacc ggttggcgtt acttactacg gtaattgacg agagcgccat
    32761 tgccgctccg gcaactaccg ggttaagcag tgttccagta aacggccaca aaataccggc
    32821 ggcgaccgga ataccgatgc tgttgtagat aaaggcaccg agcaggttct gcttcatgtt
    32881 gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg cccatcaggc tatggcgcat
    32941 cagggtaatc gccgcggttt caatggcaac atcactgccg ccacccatcg caatgccgac
    33001 atccgcctgg gccagcgctg gcgcgtcgtt aatgccgtcg cccaccattg ccacctgacg
    33061 tccttcactt tgcagacgtt tgatcgcttc ggctttaccg tccggcagca ctccggcaat
    33121 cacttcatca attccagctt ctttggcgat cgcattggcg gtggttgggt tatccccggt
    33181 caacatcacc agacgatatc ccgctttatg caggcgttgc agcgccgcca cgctatcact
    33241 acgcaacgga tcgcgtacag ccagcagggc tactgctttc ccgtcaatcg ccagcagcac
    33301 aggcgttgct ccttgcgatg cctgagctga aatatcggct tcaatcgctt tagtatcgac
    33361 ttgctgatca tttaacaacg cctgattacc cagcaataac gcatgacctt ctgcttcacc
    33421 gctcacgccc agcccgcgca atgtgcggaa accgttgacc tgcggtagct gcatatcgcc
    33481 tgctttatca aggatcgctc gtgccaatgg atggctggaa ccttgctcca gcgccgccgc
    33541 cagacgcagc gcctgcgctt cgtcaacatc agcaaatgtt ttcactgcga caacctgcgg
    33601 cttcccttcg gtcagcgtcc cggttttatc gaacacgacg gtatcgagtg tactggcgcg
    33661 ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca gccgcccgcc cgacgccgga
    33721 aataatcgac atcggcgtcg ccagtcccag cgcacacggg caggcgataa tcagcaccgt
    33781 agtggcaatc accagggtat agacaatctg cggtgccgga ccaaagaaat accagattgc
    33841 cgcactgaca agcgcaataa ccactactac cggcacaaat atggctgaga ttttatccgc
    33901 cagctgaccg atttctggct tgctgctctg ggcctggcgc accatgcgaa tgatccttga
    33961 tagcgtggta tggctgccaa ccgcactggc acgaaacagc acactgccgt cctgtaccac
    34021 tgtcccggca tggacgctat cgccttcgcc tttttgctgc gggattggtt cgcccgtcag
    34081 catcgcttca tccagccatg cttcgccctg ggtaatctcg ccatctaccg gcacgcgatc
    34141 gccggtcgtc aggcgcagca acatacctgg ctgcacttct gccagaggca cgcttttttc
    34201 accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa tcgagtaatt tctccagtgc
    34261 cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg ccgagattga tcagaccgat
    34321 aatcatcgca ctggcttcat agtaaagatg tcgcgcttcc atcgggaacc actgcggcca
    34381 caggttgacg ctcatcgaat agagccacgc cacgcccgtt cccagcgcca ccagcgtatc
    34441 catcgtcgct gcaccgttca gcaggctttt ccatgcactg cggtaaaaat ggccgctggc
    34501 gaaaaccatc accgccaggg ttatcaggcc gataaccaac cacaggctgc ggttgtcggc
    34561 ggtgactatc atgttatcgc cgatcatccc ccagaccatc accgggatac ccaccgccag
    34621 tgcgacaatt gcctgccagc ggaagcgctt catcgtagcg acggcggttt cttgctggcg
    34681 ttcgcggcgt ttcgcgtcat cttcaatcgc ttccgcgccg tagcccgctt tttccaccgc
    34741 ctgcactaaa tcttgtgggg aggcactgcc catcaccagc gcagtacgct ccgccaggtt
    34801 tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca ttttgtacgc gggtgacaca
    34861 gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg tcatcgtcat cggcggtcgc
    34921 tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac gggattgatg actccgccag
    34981 cggtttagcc tttgggtggc ttacagatgc gtcataaccc gcttgtttga tggtttcgat
    35041 cagctgttct gcactggcag tcccggtaac gtgcgcttca gtgatagaca catccgcttg
    35101 ttccacatcc gggcgctgtt caagactttc tttcacgcgt ttaacgcagt gaccgcagga
    35161 caggccgtcc aggttcaggt cgatagtttg tgacataaaa cactccttta agacagtttt
    35221 gactggctgt gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa ttaataaacc
    35281 aggcgggtaa aagtccgtaa agattaaaaa atcggctcga tttgcatcag gattagacat
    35341 ttatttcttt gttttcctgt agttaagttg cgggtgctaa gttaaatcag gatgcctgaa
    35401 aatcggcact ggggtgagga attacctccc gcatctataa aaaggagtta acaaaagatg
    35461 ttagatgcaa acaaattaca gcaggcagtg gatcaggctt acacccaatt tcactcactt
    35521 aacggcggac aaaatgccga ttacattccc tttctggcga atgtaccagg tcaactggcg
    35581 gcagtggcta tcgtgacctg cgatggcaac gtctatagtg cgggtgacag tgattaccgc
    35641 tttgcactgg aatccatctc taaagtctgt acgttagccc ttgcgttaga agatgtcggc
    35701 ccgcaggcgg tacaggacaa aattggcgct gacccgaccg gattgccctt taactcagtt
    35761 atcgccttag agttgcatgg cggcaaaccg ctttcgccac tggtaaatgc tggcgctatt
    35821 gccaccacca gcctgattaa cgctgaaaac gccgaacaac gctggcagcg aattttacat
    35881 atccaacagc aactggctgg tgagcaggta gcgctctctg acgaagtcaa ccagtcggaa
    35941 caaacaacca acttccataa tcgggccatt gcctggctgc tgtactccgc cggatatctc
    36001 tattgtgatg caatggaagc ctgtgacgtg tacacccgtc agtgctctac gctcatcaat
    36061 actgttgaac tggcaacgct tggcgcgacg ctggcggcgg gtggtgtgaa tccgttgacg
    36121 cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc tggccgaaat gatgatggaa
    36181 gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg gtttaccggg caaaagcggt
    36241 gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg gaattgccgc gttctcacca
    36301 ccgctggacg aagaaggcaa tagtgttcgc ggtcaaaaaa tggtggcatc ggtcgctaag
    36361 caactcggct ataacgtgtt taagggctga tcatgatgaa cacggaaggt aataacggta
    36421 acaaacccct cggtctatgg aacgtcgttt ccatcggtat tggggcaatg gtgggggcgg
    36481 ggatcttcgc gctgctgggg caggctgcgt tgctaatgga agcctcgacc tggggcgcct
    36541 ttgcttttgg cggtattgtg gctatgtttt ccggttatgc ctatgcgcgt ctgggggcga
    36601 gctatcccag caatggcggt attatcgact tttttcgtcg cggattaggc aacggcgtct
    36661 tttcgctagc gctctcgttg ctatacctgt tgacgctggc ggtgagcatc gccatggttg
    36721 cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga aggcagccag gaggagcacc
    36781 ttattttgct ctacgcgttg gggatcattg cggtgatgac gcttttcaac tccttaagca
    36841 accatgcggt agggcggctg gaagtgatcc tcgtcggcat taaaatgatg atcctgttat
    36901 tgctgattat tgccggtgtc tggtcgctgc aaccggcgca tatttccgtc tctgcgcccc
    36961 ccagctccgg tgcgttcttc tcctgtattg ggataacctt ccttgcctat gcgggctttg
    37021 gcatgatggc gaacgcggcg gataaagtga aagatccgca ggtcattatg ccacgggcgt
    37081 ttctggtggc gattggcgtt accacgttgc tttatatctc gctggcgctg gttttgctta
    37141 gcgatgtatc ggcattagag ttagaaaaat atgctgatac cgccgtagcg caggctgctt
    37201 ctccgctgct cgggcatgtg ggttatgtga tcgtcgtcat cggcgcttta ctggcgacgg
    37261 cttcaggcat taacgcgaac ctgttcgccg tgtttaacat catggacaac atgggcagcg
    37321 aacgcgaact gccgaagcta atgaataaat ccctgtggcg gcagagtacc tggggcaata
    37381 tcatcgtcgt ggtgttgata atgctgatga cggcggcgct aaatttagac tcactcgcca
    37441 gcgttgccag cgccaccttt ttgatctgct atctggcggt gtttgtggtg gcgatccgcc
    37501 tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt tggtacgttg gtgatgttgt
    37561 tggtgatcgt tggctttatc tacagcctgt ggtcccaggg cagccgtgcg ttgatatgga
    37621 ttattggtgc aatcttactc agccttattg tggcaatggt catgaagcgt aataaaaccg
    37681 tataacacct ctctgtgcgc agtacttcct gtattattgt ggtggcggtc gatattcgca
    37741 ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt aacaaagcac aggaggcgtt
    37801 gcgcgaacga tgaacatcag cgatgtagca aaaattaccg gcctgaccag caaagccatt
    37861 cgcttctatg aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc
    37921 acctacacgc agcagcatct caacgaactg accttactgc gccaggcacg gcaggtgggc
    37981 tttaacctgg aagagagcgg cgagctggtg aatctgttta acgacccgca gcggcacagc
    38041 gccgacgtca aacggcgcac gctggagaag gtggcggaga tcgaacgaca cattgaggag
    38101 ctgcaatcca tgcgcgacca gctgctggca ctggcgaatg cctgccctgg cgatgacagc
    38161 gccgactgcc cgattatcga aaatctctcc ggctgctgtc atcatcgggc agggtgatta
    38221 agacgagacg gctcggatat gtagggttat cccttctatc gcaatgactt caacatgcgt
    38281 acctgcaccg agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg
    38341 accgcgcccg ttgaccagcg gagattccag cacaaaacgt cggccaatca gctgctgccc
    38401 gcgctggttt aaatgactat cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt
    38461 ccaccacagc caggcggcga gcagcgtcag gatggcaaac attacccctt gccactccca
    38521 acccagcggc accagccaga ccaccaggcc ggtaatcacc gctgccacgc cgctccacaa
    38581 caaataacca tttccgccca gcatctcggc tgccagcagc aacccgccga gactgagcca
    38641 gaaaatatgt ggatgaacga ccattaactc catcatggct gagtccgttt gttggcgctg
    38701 tctttcacca gctcggcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc
    38761 atcatcacta ctttgctgtt actggaggaa ccgatctgct gtaacgcttc ggtgtatttt
    38821 tgcgctacga agtagttcac cgcctgaata tcaccggagg cgatagcttc agacaccatt
    38881 ttggtggcgc gggcttctgc ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc
    38941 gactgacgtt cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg
    39001 atttccgcct gacggatccc ttccgcttca agaatgtaag cgcgtttggt acgttccgct
    39061 ttcatctgcg cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca
    39121 atacgggtga ctttaatccc ccacgggttg gtggcttcat cgacaatacg cagcaggcgt
    39181 gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc caacacggta
    39241 cggatgttag tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataggca
    39301 gcgcgtggcg cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac gttggcgtta
    39361 tctttcgaga taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg
    39421 cgaccaatgc gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta
    39481 tagcgaccaa aacgttctac cgtccattga tagccctgcg gcacgatttt gacgcccgcg
    39541 ccgacaatga ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc
    39601 tcctgttgta ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat
    39661 tactgaaaga gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag
    39721 aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc agctacaaaa cgtaggatat
    39781 ctggcgggtg atacgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt
    39841 aagttaatta ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat agttgcttca
    39901 ttgatcagcc caaccagcgg aacgttactg tttgaaggtg aggatgtcag cacactaaaa
    39961 ccagaaatct accgccagca agtctcttac tgcgcccaga cgccgacgct gtttggcgat
    40021 acggtatacg ataatctgat ctttccctgg cagatccgtg accagcagcc tgacccggcc
    40081 atttttctcg attttctcga acgcttcgct ttgccggaca gcattttgac gaagaatatc
    40141 gccgagctat ctggtggtga aaaacaacgc atctcattga ttcgtaacct gcaatttatg
    40201 ccgaaggttt tattgctgga tgaaataacc agtgcgctgg atgaaagtaa taaacataac
    40261 gtcaatgaga tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca
    40321 cacgataaag acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga
    40381 gaaatgcagg aagcacgcta tgaactcgca taatattact aacgaatcat tagcactggc
    40441 attaatgctg gtggtggtgg caatcttaat tagccataaa gaaaaactgg cgctggagaa
    40501 agatattcta tggagcgtcg ggcgagcgat aattcagctg attattgtcg gctatgtgct
    40561 gaagtatatt ttcagcgtgg atgatgccag cctgacatta ttgatggtgt tatttatctg
    40621 ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag cttttatctc
    40681 atcgtttatt gccattacgg tcggggcggg aattaccctg gcggtgctga ttctctcagg
    40741 ggcgattgaa tttatcccga tgcaggtgat ccctgtcgcc gggatgattg ccggtaacgc
    40801 catggtagcg gtggggttgt gttacaacaa tttagggcaa cgggtcatta gcgaacagca
    40861 acagattcag gagaaactga gtcttggcgc gacgccgaag caggcttcag cgatattgat
    40921 tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa cggttggctt
    40981 agtgagttta ccaggaatga tgtccgggct gatatttgcc gggattgatc cggtgaaggc
    41041 gattaaatat cagattatgg tgacctttat gctgctctca accgccagct tgtcgaccat
    41101 tattgcctgc tatttaacct atcgtaagtt ttataattcg cgccaccagt tggtggtgac
    41161 tcaattgaag aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt
    41221 catgtgccga acaacggaat atcaatacaa caatgcatac agctggcggc gatacttcga
    41281 cgccagtgca tcacccgtac ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg
    41341 accgtctgcg gcggtgagat ctttacgcag atgcccgaac agcaactcca gcgcctcttc
    41401 attgcgccca acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg
    41461 attctccgcc acctgctgtt gcaactgttg aatttccggc ttatcagccg cctgcttcag
    41521 cagttcgatt tgcgctacca gcccctggta gcgggtgtcc tgatcctgca acggaatggt
    41581 tttcagcacc gcttccgcat cttcagaacg gttcagcgca atcagcgttt ctgccagcag
    41641 caggccgatc tccccgttct gattcgacaa ctgccaggcg tctttcagca atggcagggc
    41701 atcggtgtaa ttgccttcct gcatcagttg catcgcctgc tgcgctttca gctcttcttc
    41761 gcgcggcagc actttatcca gcagggcgcg gatcgcctct tccggttgcg gcccctggaa
    41821 gccatctacc ggttgcccgt tctggaacag atacacggtc ggaatcgcgc gcagaccaaa
    41881 ctgggcggca atcatctgct ccgcgtcgca gtccagcttc gccagaataa attgcccgtt
    41941 gtactgcgcc gcgaggcttt ccaggatcgg ggttaactgc aaacagtgct ggctacgttc
    42001 agaccaaaaa tagaacagca ccggagtggt catcgactgt tcaagaacct gttgcaggtt
    42061 agattcgtta atgttgacaa tattttctac ggacatggag tcgctctctg ttgtcgatat
    42121 tttctttgac atgggggctt aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat
    42181 cacgcgccca ggcagcaggc gctcgagcac cattactgcc caggtcacca gcgttaccgg
    42241 atagcgcatc ttcggcttct cgctaataaa agcatggcgc actttgtcga ccaccgcttc
    42301 cggtcccaac gtaaagcggg cggcgatgcc gggattttcg actggtttat cactttgcgt
    42361 ctggttgacg ttgtcggtga agcgagtacg aatgggaccg ggttcgatca ggctgacttt
    42421 aattccgctg tggcgtagct ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt
    42481 actggccgcg taagcgccac gacccggcgt ggagattaat cccatcaccg atgatgtcat
    42541 cacaatacgc ccttcaccgt gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg
    42601 tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc gcacggctga tggtggaaag
    42661 ggggccatac atgccgaatc cggcattgtt aaagatccca tacagacaat tatcggtcag
    42721 ggcgatcacc tcgtctgctg cgcgattaac actttctggt gaatccagat cgatcaacac
    42781 gccggtaaat cccatgttgt tcatacgctc aacatcattc ggttttcggc aacctgccag
    42841 cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc aggccaattc cactggaaca
    42901 tccggtaatt aagaccgatt tttgcataac tttacctgtc aggatctccg ttgctttatg
    42961 agtcatgatt tactaaaggc tgcaactgct tcgccatcca gtcggcaata aacggctggg
    43021 cgtcgcggtt gggatgaata ccgtcatcct gcatccattg tggcttgagg tagacctctt
    43081 ccataaaaaa gggcagcagc ggaacatcaa actctttggc gagtttgggg taaatggcgc
    43141 taaaggcttc attataacgg cgaccatagt ttgcaggcag acgtatttgc attaacaatg
    43201 gttcagcgtt ggcggctttg acatcctgca aaatctggcg cagcgtttgc tcggtttgct
    43261 gtggctgaaa accacgcaaa ccatcattgc gcccagttca accagcaccc aacgcggctg
    43321 atgctgtttg agcagagccg gaaggcgcgc cagcccttgt tgcgaggtgt cgccgctgat
    43381 gctggcatta actaccgacg ttttactctg ccacttatca ttcaacaagg caggccaggc
    43441 cgcgctggca gacattcgat acccggcgct caggctatca cccagaatca ataacgtgtc
    43501 cgctgcggcg gcacggaagg ttaacaggac caggaacagg aagggcaaat gccagcggaa
    43561 aacattgttg aagttcatca tcttaagaag tccgtcggtc agggggagca tgaactctcc
    43621 atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga ccatcgcact ggtgggcgag
    43681 tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg ggcttgatga cggcagcagt
    43741 ggcgaagtga gtctggtggg acaaccgcta cataatatgg acgaagaagc gcgggcaaag
    43801 ttgcgcgcga agcacgtcgg ctttgttttt cagtcattta tgttaattcc tacccttaac
    43861 gcgctggaaa acgtcgagct tccggctctg ctgcgcggtg agagtagcgc ggaaagtcgt
    43921 aacggggcga aagcgttgct cgaacagtta gggctgggta aacgtctgga tcatcttccg
    43981 gcacagcttt ccggcggtga acagcaacga gtggcgctgg cacgagcctt taatggtcga
    44041 cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg accgccagac gggcgataaa
    44101 attgccgacc tgctgttttc cctcaaccgt gaacatggca ccacgttgat tatggtgacc
    44161 cacgacctgc aactggcggc acgctgcgac cgctgcttac ggctggtgaa cgggcagttg
    44221 caggaggaag catgattgca cgttggttct ggcgcgaatg gcgttcgccg tcgctattaa
    44281 ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct ggcgctgggc aatatcagcg
    44341 atcgcatgga gaagggctta agccagcaaa gccgtgagtt tatggcgggc gatcgggcgt
    44401 tgcgcagttc acgcgaagtg ccgcaagcgt ggctggagga agcgcaaaag cgcggcctga
    44461 aagtcggcaa gcagctgact ttcgccacaa tgacctttgc aggcgacaca ccgcagctgg
    44521 cgaacgtcaa agcggtggat gatatctacc cgatgtatgg cggtctgcaa actaatcccc
    44581 ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc acgcctgatg gcactgctta
    44641 acctgaaaac gggcgacacc attgacgtgg gcgatgccac cttgcggatt gccggagaag
    44701 tgattcagga accggattcc ggttttaacc ccttccagat ggctccgcgt ctgatgatga
    44761 atctggcgga tgtcgataaa accggagccg tgcagccggg gagtcgggtc acctggcgtt
    44821 ataaattcgg cggcaacgag aaccagctcg acggctatga gaaatggttg ttacctcagc
    44881 ttaaacccga acaacgctgg tacggtctgg aacaggacga aggcgcgctg gggcgatcga
    44941 tggaacgctc gcaacagttc ctgctgcttt cggcgcttct gaccttgctg ctggcagtgg
    45001 cagcggtggc ggtagcgatg aatcattact gtcgcagtcg ctacgatctg gtggcgatcc
    45061 tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagct aatcgtcggt cagtggttga
    45121 tggtgctgac gctttcagcc gttaccggtg gggccatagg cctgttgttc gaaaacgtgt
    45181 tgatggtgct gctcaagccc gttctgcctg ctgcactacc gccagccagc ctctggccgt
    45241 ggctgtgggc gcttggcacc atgacggtca tctcgctgct ggtggggcta cgaccatacc
    45301 gcttgttgct ggcaacgcag cctttacgcg tattacgtaa tgatgtggtc gcgaacgtct
    45361 ggccgctgaa gttttatctg ccgattgtca gtgtggtggt tgtgctgctg ctcgccggat
    45421 taatgggtgg cagcatgctg ctttgggcgg tgctggcggg cgcggtagta ctggctttgc
    45481 tgtgcggtgt gctgggctgg atgctgctga atgtacttcg ccgcatgacg ctgaaatcgc
    45541 tgcctctgcg cctggcggtt agccgcctgt tacgtcagcc gtggtcaacg ttaagccagc
    45601 tttcggcatt ttcgctctcc tttatgctgc tggcactgct gctggtgctg cgtggcgatc
    45661 tgctcgaccg ctggcagcag cagctaccgc cagaaagccc gaactacttt ttaattaaca
    45721 tcgccacaga acaggtagca ccgctaaaag cgttcctcgc ggaacatcat atagtcccgg
    45781 aatcatttta tccggtagtg cgggcgcggc tgacggcgat taatgataag ccgacagaag
    45841 gtaatgaaga tgaggcgctt aatcgcgaac tcaatcttac ctggcaaaat acgcggcccg
    45901 atcataatcc gattgtcgcc ggtaactggc cgccaaaagc cgatgaagtg tcgatggaag
    45961 aggggctggc gaaacgctta aacgttgccc tcggtgatac cgtgactttt atgggcgata
    46021 cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt ggactgggaa agtctgcggc
    46081 ctaatttcta ttttattttc cctgaagggg cattagacgg gcaaccgcag agctggctta
    46141 ccagtttccg ctgggagaat ggcaacggca tgttgacgca actcaaccgc cagttcccga
    46201 ccattagcct gttagacatt ggcgcgattt taaaacaggt cggtcaggtg ctggagcagg
    46261 taagtcgggc gctggaggtg atggtggtgc tggtcaccgc ctgcggtatg ttgctgttgc
    46321 tggcacaggt gcaggtggga atgcgccagc gtcatcagga gctggtggta tggcgcacac
    46381 tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg tgatgttcgc catgcttggg
    46441 tttgtttccg gcctggtggc cgcaattggt gcggaaacgg cactggcagt gttgcaggcg
    46501 aaagtgtttg atttcccgtg ggagccagac tggcgattgt ggattgttct gccgtgcagc
    46561 ggtgcgctgc tgctgtcgct tttcggcggc tggctgggtg cgcgactggt taagggtaag
    46621 gcgctgttca ggcagtttgt ggggtgatga caggtgttag attgcctgat gcgctacgct
    46681 tatcaggcct acgaagagcg tgcaatatat tgattttgca agattgtgta ggccggataa
    46741 ggcgtttacg ccgcatctgg catgaacagt gtgcattttg gcaataaata tgaaaccaac
    46801 tgtcatgtcg gtttttttaa aggccgaagg aggtaaacct tcggccttag ctacgtttcc
    46861 tcagttattc aataaactga cggatcagtt ccagcagcgc gattagcgcc ttaagaacga
    46921 tgagaacttt atcgagaaaa gttttcatcc gttgctccct taagggagaa agacaatcac
    46981 agcttaccct tacgttgcct ataactatga ttttcttcat gtgctagatg aggactgtct
    47041 ctctccaaat tagagcacca gaatcagcac cacctgtatc ctgaccagga cttaaaagta
    47101 cttttccgca ctgcggtaat cccttgaggc agtatcacta ctagcgtcgg gattgtatcg
    47161 ccaacatagc tgtttgaata tgtaggcatc ctgacttata attcacttgc tagatgagga
    47221 ctgtttaatt tctgcccgga accaccaagt cttgggcgga aaaaataaaa aaaccgactg
    47281 tcatgtcggt ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attaatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ttcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caactaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg gggggttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt cgttggt
//
LOCUS       DAJTRH010000018        52660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTRH010000018 DAJTRH010000000
VERSION     DAJTRH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..354
                     /locus_tag="OO339_001154"
     CDS             <1..354
                     /locus_tag="OO339_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5925861.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO339_001155"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO339_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5925862.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO339_001156"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO339_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5925863.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OO339_001157"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OO339_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5925864.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO339_001158"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO339_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5925865.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO339_001159"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO339_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5925866.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OO339_001160"
     CDS             6877..7281
                     /locus_tag="OO339_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5925867.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OO339_001161"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OO339_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5925868.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OO339_001162"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OO339_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5925869.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO339_001163"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO339_001163"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5925870.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO339_001164"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO339_001164"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5925871.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12134
                     /locus_tag="OO339_001165"
                     /pseudo
     CDS             10704..12134
                     /locus_tag="OO339_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12204..12980
                     /gene="yaaA"
                     /locus_tag="OO339_001166"
     CDS             12204..12980
                     /gene="yaaA"
                     /locus_tag="OO339_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5925872.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13133..13429)
                     /locus_tag="OO339_001167"
     CDS             complement(13133..13429)
                     /locus_tag="OO339_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5925873.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO339_001168"
     CDS             complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO339_001168"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5925874.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO339_001169"
     CDS             complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO339_001169"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5925875.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO339_001170"
     CDS             complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO339_001170"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5925876.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGIYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18352..18471)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO339_001171"
     CDS             complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO339_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5925877.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18686..19372)
                     /locus_tag="OO339_001172"
     CDS             complement(18686..19372)
                     /locus_tag="OO339_001172"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5925878.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19772..19912)
                     /locus_tag="OO339_001173"
     CDS             complement(19772..19912)
                     /locus_tag="OO339_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925879.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20008..20724
                     /gene="arcA"
                     /locus_tag="OO339_001174"
     CDS             20008..20724
                     /gene="arcA"
                     /locus_tag="OO339_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5925880.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO339_001175"
     CDS             complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO339_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5925881.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO339_001176"
     CDS             complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO339_001176"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5925882.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO339_001177"
     CDS             complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO339_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5925883.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO339_001178"
     CDS             complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO339_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5925884.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25003..25872
                     /gene="robA"
                     /locus_tag="OO339_001179"
     CDS             25003..25872
                     /gene="robA"
                     /locus_tag="OO339_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5925885.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO339_001180"
     CDS             complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO339_001180"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5925886.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26568..27088
                     /gene="yjjX"
                     /locus_tag="OO339_001181"
                     /pseudo
     CDS             26568..27088
                     /gene="yjjX"
                     /locus_tag="OO339_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO339_001182"
     CDS             complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO339_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5925887.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO339_001183"
     CDS             complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO339_001183"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5925888.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29737..31404
                     /gene="ettA"
                     /locus_tag="OO339_001184"
     CDS             29737..31404
                     /gene="ettA"
                     /locus_tag="OO339_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5925889.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31511..31678)
                     /locus_tag="OO339_001185"
     CDS             complement(31511..31678)
                     /locus_tag="OO339_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925890.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO339_001186"
     CDS             complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO339_001186"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5925891.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO339_001187"
     CDS             complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO339_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5925892.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO339_001188"
     CDS             complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO339_001188"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5925893.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35470..36114
                     /locus_tag="OO339_001189"
     CDS             35470..36114
                     /locus_tag="OO339_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5925894.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36142..37157
                     /gene="lplA"
                     /locus_tag="OO339_001190"
                     /pseudo
     CDS             36142..37157
                     /gene="lplA"
                     /locus_tag="OO339_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37189..37452
                     /locus_tag="OO339_001191"
     CDS             37189..37452
                     /locus_tag="OO339_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5925895.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO339_001192"
     CDS             complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO339_001192"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5925896.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO339_001193"
     CDS             complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO339_001193"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5925897.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO339_001194"
     CDS             complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO339_001194"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5925898.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO339_001195"
     CDS             complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO339_001195"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5925899.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42150..43691
                     /locus_tag="OO339_001196"
     CDS             42150..43691
                     /locus_tag="OO339_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5925900.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43663..44526
                     /locus_tag="OO339_001197"
     CDS             43663..44526
                     /locus_tag="OO339_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5925901.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44740..45522)
                     /locus_tag="OO339_001198"
     CDS             complement(44740..45522)
                     /locus_tag="OO339_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5925902.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45519..46592)
                     /locus_tag="OO339_001199"
     CDS             complement(45519..46592)
                     /locus_tag="OO339_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5925903.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46714..46875)
                     /locus_tag="OO339_001200"
     CDS             complement(46714..46875)
                     /locus_tag="OO339_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5925904.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO339_001201"
     CDS             complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO339_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5925905.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO339_001202"
     CDS             complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO339_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5925906.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO339_001203"
     CDS             complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO339_001203"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5925907.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO339_001204"
     CDS             complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO339_001204"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5925908.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO339_001205"
     CDS             complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO339_001205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5925909.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51293..52324
                     /gene="rsmC"
                     /locus_tag="OO339_001206"
     CDS             51293..52324
                     /gene="rsmC"
                     /locus_tag="OO339_001206"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5925910.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12538 a        14244 c        13204 g        12674 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagggaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcca ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc agtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgtgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 tgggaatggg atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg
    11581 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    11641 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    11701 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
    11761 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    11821 acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    11881 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    11941 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    12001 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    12061 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    12121 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    12181 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    12241 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    12301 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    12361 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    12421 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    12481 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    12541 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    12601 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    12661 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    12721 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    12781 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    12841 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    12901 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
    12961 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    13021 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
    13081 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
    13141 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
    13201 gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13261 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
    13321 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
    13381 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13441 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
    13501 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
    13561 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    13621 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
    13681 aatcggcggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13741 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13801 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    13861 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13921 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13981 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    14041 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    14101 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
    14161 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14221 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14281 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
    14341 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    14401 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14461 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
    14521 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
    14581 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    14641 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14701 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
    14761 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14821 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14881 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
    14941 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    15001 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
    15061 cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15121 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
    15181 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
    15241 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15301 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
    15361 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15421 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
    15481 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
    15541 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15601 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    15661 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgagtt
    15721 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15781 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15841 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15901 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15961 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    16021 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
    16081 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16141 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16201 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16261 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16321 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
    16381 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
    16441 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16501 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
    16561 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16621 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16681 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16741 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
    16801 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
    16861 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16921 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16981 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    17041 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17101 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17161 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    17221 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17281 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
    17341 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17401 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    17461 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17521 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17581 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gatcccgtca
    17641 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17701 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    17761 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
    17821 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    17881 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17941 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    18001 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18061 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18121 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
    18181 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18241 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18301 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18361 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18421 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18481 tgtactctgt aatttttatc tgtttgtgcg ctatgcctat attggttaaa gtatttagtg
    18541 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18601 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18661 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18721 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    18781 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    18841 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18901 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18961 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
    19021 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19081 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    19141 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19201 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    19261 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    19321 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    19381 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    19441 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19501 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    19561 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    19621 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    19681 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    19741 ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga
    19801 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    19861 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    19921 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    19981 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    20041 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    20101 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    20161 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    20221 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    20281 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    20341 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    20401 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    20461 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    20521 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    20581 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    20641 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    20701 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    20761 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    20821 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    20881 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    20941 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca
    21001 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    21061 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    21121 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    21181 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    21241 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    21301 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    21361 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    21421 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    21481 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    21541 tattcagctg caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
    21601 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    21661 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    21721 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    21781 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    21841 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    21901 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    21961 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    22021 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    22081 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    22141 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    22201 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    22261 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    22321 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    22381 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    22441 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    22501 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    22561 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    22621 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    22681 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    22741 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    22801 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    22861 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    22921 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    22981 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    23041 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    23101 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    23161 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    23221 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    23281 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    23341 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    23401 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    23461 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    23521 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    23581 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    23641 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    23701 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    23761 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    23821 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    23881 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    23941 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    24001 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    24061 cgtcaggaac agtacaggta acgccggata gagcgccagt aactggcggc acaattcaaa
    24121 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    24181 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    24241 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    24301 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    24361 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    24421 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    24481 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    24541 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    24601 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    24661 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    24721 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    24781 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    24841 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    24901 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    24961 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    25021 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    25081 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    25141 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    25201 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    25261 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    25321 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    25381 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    25441 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    25501 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    25561 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    25621 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    25681 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    25741 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    25801 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    25861 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    25921 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    25981 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    26041 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    26101 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    26161 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    26221 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    26281 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    26341 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    26401 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    26461 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    26521 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    26581 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    26641 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    26701 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    26761 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    26821 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    26881 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    26941 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    27001 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    27061 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    27121 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    27181 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    27241 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    27301 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    27361 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    27421 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    27481 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    27541 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    27601 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    27661 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    27721 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    27781 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    27841 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    27901 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    27961 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    28021 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    28081 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    28141 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    28201 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    28261 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    28321 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    28381 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    28441 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    28501 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    28561 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    28621 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    28681 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    28741 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    28801 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    28861 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    28921 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    28981 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    29041 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    29101 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    29161 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    29221 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    29281 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    29341 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    29401 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    29461 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    29521 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    29581 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    29641 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    29701 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    29761 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    29821 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    29881 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    29941 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    30001 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    30061 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    30121 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    30181 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    30241 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    30301 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    30361 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    30421 atcctcgaac tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg
    30481 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    30541 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    30601 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    30661 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    30721 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    30781 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    30841 atctccggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    30901 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    30961 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    31021 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    31081 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    31141 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    31201 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    31261 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    31321 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    31381 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    31441 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    31501 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    31561 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    31621 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    31681 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    31741 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    31801 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    31861 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    31921 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    31981 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    32041 cgtatttcac tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg
    32101 aatactgcaa tgcgatttca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    32161 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    32221 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    32281 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    32341 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    32401 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    32461 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    32521 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    32581 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    32641 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    32701 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    32761 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    32821 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    32881 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    32941 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    33001 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    33061 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    33121 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    33181 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc
    33241 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    33301 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    33361 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    33421 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    33481 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    33541 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    33601 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    33661 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    33721 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    33781 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    33841 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    33901 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    33961 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    34021 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    34081 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    34141 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    34201 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    34261 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    34321 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    34381 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    34441 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    34501 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    34561 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    34621 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    34681 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    34741 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    34801 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    34861 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    34921 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    34981 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    35041 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    35101 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    35161 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    35221 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    35281 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    35341 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    35401 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    35461 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    35521 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    35581 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    35641 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    35701 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    35761 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    35821 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    35881 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    35941 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    36001 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    36061 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    36121 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    36181 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    36241 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    36301 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    36361 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    36421 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    36481 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    36541 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    36601 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    36661 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    36721 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    36781 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    36841 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    36901 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    36961 gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc
    37021 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    37081 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    37141 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    37201 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    37261 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    37321 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    37381 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    37441 gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat
    37501 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    37561 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    37621 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    37681 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    37741 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    37801 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    37861 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    37921 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    37981 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    38041 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    38101 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    38161 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    38221 gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    38281 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    38341 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    38401 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    38461 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg
    38521 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    38581 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    38641 cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa
    38701 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    38761 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    38821 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    38881 ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg
    38941 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    39001 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    39061 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    39121 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    39181 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    39241 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    39301 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    39361 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    39421 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    39481 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    39541 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    39601 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    39661 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag
    39721 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag
    39781 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    39841 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac
    39901 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    39961 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    40021 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    40081 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat
    40141 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    40201 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    40261 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    40321 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    40381 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    40441 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    40501 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    40561 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    40621 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    40681 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    40741 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    40801 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    40861 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    40921 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    40981 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    41041 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    41101 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    41161 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    41221 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    41281 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    41341 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    41401 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    41461 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    41521 ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa
    41581 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    41641 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    41701 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    41761 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    41821 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    41881 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    41941 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    42001 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    42061 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    42121 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    42181 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    42241 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    42301 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    42361 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    42421 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    42481 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    42541 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    42601 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    42661 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    42721 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    42781 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    42841 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    42901 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    42961 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    43021 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    43081 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    43141 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    43201 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    43261 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    43321 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    43381 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    43441 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    43501 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    43561 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    43621 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    43681 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    43741 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    43801 tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    43861 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    43921 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    43981 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    44041 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    44101 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    44161 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    44221 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    44281 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    44341 gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat
    44401 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    44461 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    44521 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    44581 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    44641 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    44701 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    44761 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    44821 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    44881 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    44941 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    45001 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    45061 accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    45121 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    45181 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    45241 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    45301 gctatgtttt tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt
    45361 ttccgctaac gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    45421 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    45481 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    45541 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    45601 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    45661 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    45721 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    45781 aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat
    45841 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    45901 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    45961 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    46021 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    46081 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    46141 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    46201 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    46261 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    46321 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    46381 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    46441 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    46501 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    46561 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    46621 aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    46681 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    46741 actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    46801 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    46861 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    46921 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    46981 gggaggacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    47041 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    47101 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    47161 agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct
    47221 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    47281 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    47341 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    47401 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    47461 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    47521 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    47581 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    47641 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    47701 tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat
    47761 cggtagaatg acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac
    47821 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    47881 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    47941 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    48001 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    48061 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    48121 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    48181 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    48241 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    48301 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    48361 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    48421 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    48481 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    48541 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    48601 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca
    48661 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    48721 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    48781 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    48841 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    48901 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    48961 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    49021 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg
    49081 cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat
    49141 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    49201 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    49261 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    49321 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    49381 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    49441 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    49501 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca
    49561 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    49621 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    49681 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    49741 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    49801 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    49861 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    49921 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    49981 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca
    50041 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    50101 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    50161 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    50221 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    50281 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    50341 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    50401 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    50461 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    50521 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    50581 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt
    50641 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    50701 ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg
    50761 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    50821 gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg
    50881 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    50941 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    51001 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    51061 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    51121 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    51181 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    51241 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    51301 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    51361 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    51421 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    51481 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    51541 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    51601 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    51661 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    51721 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    51781 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    51841 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    51901 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    51961 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    52021 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    52081 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    52141 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    52201 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    52261 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    52321 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    52381 acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc
    52441 tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc
    52501 cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg
    52561 cgcctccgtg gtagcaattc tttttaagaa ttgatggtat gcgaaggtgg cggaattggt
    52621 agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg
//
LOCUS       DAJTRH010000019        49759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTRH010000019 DAJTRH010000000
VERSION     DAJTRH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..1224
                     /locus_tag="OO339_001207"
     CDS             <1..1224
                     /locus_tag="OO339_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5925911.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO339_001208"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO339_001208"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5925912.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OO339_001209"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OO339_001209"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5925913.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OO339_001210"
     CDS             2714..3427
                     /locus_tag="OO339_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5925914.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="OO339_001211"
     CDS             complement(3399..4076)
                     /locus_tag="OO339_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5925915.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="OO339_001212"
     CDS             complement(4070..4747)
                     /locus_tag="OO339_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5925916.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="OO339_001213"
     CDS             complement(4735..5118)
                     /locus_tag="OO339_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5925917.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="OO339_001214"
     CDS             complement(5134..5442)
                     /locus_tag="OO339_001214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925918.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="OO339_001215"
     CDS             complement(5443..6021)
                     /locus_tag="OO339_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5925919.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="OO339_001216"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="OO339_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="OO339_001217"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="OO339_001217"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5925920.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="OO339_001218"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="OO339_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5925921.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="OO339_001219"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="OO339_001219"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5925922.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="OO339_001220"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="OO339_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5925923.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="OO339_001221"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="OO339_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5925924.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="OO339_001222"
     CDS             12291..13031
                     /locus_tag="OO339_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5925925.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="OO339_001223"
     CDS             13198..14148
                     /locus_tag="OO339_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5925926.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO339_001224"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO339_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO339_001225"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO339_001225"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5925927.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO339_001226"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO339_001226"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5925928.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO339_001227"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO339_001227"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5925929.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO339_001228"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO339_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5925930.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="OO339_001229"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="OO339_001229"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5925931.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="OO339_001230"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="OO339_001230"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5925932.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="OO339_001231"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="OO339_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5925933.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="OO339_001232"
     CDS             23768..23827
                     /locus_tag="OO339_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5925934.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="OO339_001233"
     CDS             complement(23928..24530)
                     /locus_tag="OO339_001233"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5925935.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="OO339_001234"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO339_001234"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO339_001235"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO339_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5925936.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="OO339_001236"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="OO339_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5925937.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="OO339_001237"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="OO339_001237"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5925938.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="OO339_001238"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="OO339_001238"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5925939.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="OO339_001239"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="OO339_001239"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5925940.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="OO339_001240"
     CDS             complement(29676..29759)
                     /locus_tag="OO339_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925941.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="OO339_001241"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="OO339_001241"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5925942.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="OO339_001242"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="OO339_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5925943.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="OO339_001243"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="OO339_001243"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5925944.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="OO339_001244"
     CDS             34816..35559
                     /locus_tag="OO339_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5925945.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO339_001245"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO339_001245"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5925946.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /locus_tag="OO339_001246"
                     /pseudo
     CDS             37094..37404
                     /locus_tag="OO339_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37568..38227
                     /locus_tag="OO339_001247"
     CDS             37568..38227
                     /locus_tag="OO339_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5925947.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO339_001248"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO339_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5925948.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="OO339_001249"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="OO339_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5925949.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="OO339_001250"
     CDS             39893..40300
                     /locus_tag="OO339_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5925950.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO339_001251"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO339_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5925951.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="OO339_001252"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="OO339_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5925952.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="OO339_001253"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="OO339_001253"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5925953.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="OO339_001254"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="OO339_001254"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5925954.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="OO339_001255"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="OO339_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5925955.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="OO339_001256"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="OO339_001256"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5925956.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO339_001257"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO339_001257"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5925957.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="OO339_001258"
     CDS             47705..47776
                     /locus_tag="OO339_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925958.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /locus_tag="OO339_001259"
     CDS             complement(47773..47898)
                     /locus_tag="OO339_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5925959.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO339_001260"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO339_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5925960.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12028 a        12440 c        13388 g        11903 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 tcgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacata
//
LOCUS       DAJTRH010000020        48991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTRH010000020 DAJTRH010000000
VERSION     DAJTRH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..789)
                     /locus_tag="OO339_001261"
     CDS             complement(<1..789)
                     /locus_tag="OO339_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5925961.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAG"
     gene            1157..2287
                     /gene="fruB"
                     /locus_tag="OO339_001262"
     CDS             1157..2287
                     /gene="fruB"
                     /locus_tag="OO339_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5925962.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2287..3225
                     /gene="fruK"
                     /locus_tag="OO339_001263"
     CDS             2287..3225
                     /gene="fruK"
                     /locus_tag="OO339_001263"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5925963.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3242..4933
                     /gene="fruA"
                     /locus_tag="OO339_001264"
     CDS             3242..4933
                     /gene="fruA"
                     /locus_tag="OO339_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5925964.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5357..6298
                     /locus_tag="OO339_001265"
     CDS             5357..6298
                     /locus_tag="OO339_001265"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5925965.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6286..7224
                     /gene="psuG"
                     /locus_tag="OO339_001266"
     CDS             6286..7224
                     /gene="psuG"
                     /locus_tag="OO339_001266"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5925966.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7318..8568
                     /locus_tag="OO339_001267"
     CDS             7318..8568
                     /locus_tag="OO339_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5925967.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8639..9333)
                     /gene="yeiL"
                     /locus_tag="OO339_001268"
                     /pseudo
     CDS             complement(8639..9333)
                     /gene="yeiL"
                     /locus_tag="OO339_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9464..10405
                     /gene="rihB"
                     /locus_tag="OO339_001269"
     CDS             9464..10405
                     /gene="rihB"
                     /locus_tag="OO339_001269"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5925968.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10505..11755
                     /locus_tag="OO339_001270"
     CDS             10505..11755
                     /locus_tag="OO339_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5925969.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(11811..12899)
                     /locus_tag="OO339_001271"
     CDS             complement(11811..12899)
                     /locus_tag="OO339_001271"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5925970.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(12902..13759)
                     /gene="nfo"
                     /locus_tag="OO339_001272"
     CDS             complement(12902..13759)
                     /gene="nfo"
                     /locus_tag="OO339_001272"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5925971.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(13833..14882)
                     /locus_tag="OO339_001273"
     CDS             complement(13833..14882)
                     /locus_tag="OO339_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5925972.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            14981..15862
                     /locus_tag="OO339_001274"
     CDS             14981..15862
                     /locus_tag="OO339_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5925973.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16068..17537
                     /gene="lysP"
                     /locus_tag="OO339_001275"
     CDS             16068..17537
                     /gene="lysP"
                     /locus_tag="OO339_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5925974.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            17829..19820
                     /gene="cirA"
                     /locus_tag="OO339_001276"
                     /pseudo
     CDS             17829..19820
                     /gene="cirA"
                     /locus_tag="OO339_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(19852..20687)
                     /gene="yeiG"
                     /locus_tag="OO339_001277"
                     /pseudo
     CDS             complement(19852..20687)
                     /gene="yeiG"
                     /locus_tag="OO339_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            20945..21613
                     /gene="folE"
                     /locus_tag="OO339_001278"
     CDS             20945..21613
                     /gene="folE"
                     /locus_tag="OO339_001278"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5925975.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21630..22706
                     /locus_tag="OO339_001279"
     CDS             21630..22706
                     /locus_tag="OO339_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5925976.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            22848..23888
                     /gene="galS"
                     /locus_tag="OO339_001280"
     CDS             22848..23888
                     /gene="galS"
                     /locus_tag="OO339_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5925977.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24168..25166
                     /gene="mglB"
                     /locus_tag="OO339_001281"
     CDS             24168..25166
                     /gene="mglB"
                     /locus_tag="OO339_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5925978.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25227..26747
                     /gene="mglA"
                     /locus_tag="OO339_001282"
     CDS             25227..26747
                     /gene="mglA"
                     /locus_tag="OO339_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5925979.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            26763..27773
                     /gene="mglC"
                     /locus_tag="OO339_001283"
     CDS             26763..27773
                     /gene="mglC"
                     /locus_tag="OO339_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5925980.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(27809..29022)
                     /gene="preA"
                     /locus_tag="OO339_001284"
                     /pseudo
     CDS             complement(27809..29022)
                     /gene="preA"
                     /locus_tag="OO339_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29016..30254)
                     /gene="preT"
                     /locus_tag="OO339_001285"
     CDS             complement(29016..30254)
                     /gene="preT"
                     /locus_tag="OO339_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5925981.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30448..30687)
                     /locus_tag="OO339_001286"
     CDS             complement(30448..30687)
                     /locus_tag="OO339_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5925982.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(30690..31409)
                     /gene="sanA"
                     /locus_tag="OO339_001287"
     CDS             complement(30690..31409)
                     /gene="sanA"
                     /locus_tag="OO339_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5925983.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31559..32443)
                     /gene="cdd"
                     /locus_tag="OO339_001288"
     CDS             complement(31559..32443)
                     /gene="cdd"
                     /locus_tag="OO339_001288"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5925984.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32573..33268)
                     /locus_tag="OO339_001289"
     CDS             complement(32573..33268)
                     /locus_tag="OO339_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5925985.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33265..33663)
                     /locus_tag="OO339_001290"
     CDS             complement(33265..33663)
                     /locus_tag="OO339_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5925986.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            33902..34849
                     /gene="dusC"
                     /locus_tag="OO339_001291"
     CDS             33902..34849
                     /gene="dusC"
                     /locus_tag="OO339_001291"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5925987.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35220..35303)
                     /gene="yohP"
                     /locus_tag="OO339_001292"
     CDS             complement(35220..35303)
                     /gene="yohP"
                     /locus_tag="OO339_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5925988.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35527..36963
                     /gene="mdtQ"
                     /locus_tag="OO339_001293"
                     /pseudo
     CDS             35527..36963
                     /gene="mdtQ"
                     /locus_tag="OO339_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37016..37777
                     /locus_tag="OO339_001294"
                     /pseudo
     CDS             37016..37777
                     /locus_tag="OO339_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(37894..38472)
                     /locus_tag="OO339_001295"
     CDS             complement(37894..38472)
                     /locus_tag="OO339_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5925989.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            38642..39229
                     /locus_tag="OO339_001296"
     CDS             38642..39229
                     /locus_tag="OO339_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5925990.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39403..40335
                     /gene="pbpG"
                     /locus_tag="OO339_001297"
     CDS             39403..40335
                     /gene="pbpG"
                     /locus_tag="OO339_001297"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5925991.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40373..42088)
                     /gene="dld"
                     /locus_tag="OO339_001298"
     CDS             complement(40373..42088)
                     /gene="dld"
                     /locus_tag="OO339_001298"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5925992.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42284..44581
                     /gene="bglX"
                     /locus_tag="OO339_001299"
     CDS             42284..44581
                     /gene="bglX"
                     /locus_tag="OO339_001299"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5925993.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            44833..45750
                     /gene="osmF"
                     /locus_tag="OO339_001300"
     CDS             44833..45750
                     /gene="osmF"
                     /locus_tag="OO339_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5925994.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            45757..46914
                     /gene="yehY"
                     /locus_tag="OO339_001301"
     CDS             45757..46914
                     /gene="yehY"
                     /locus_tag="OO339_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5925995.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            46907..47833
                     /gene="yehX"
                     /locus_tag="OO339_001302"
     CDS             46907..47833
                     /gene="yehX"
                     /locus_tag="OO339_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5925996.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            47838..48569
                     /gene="yehW"
                     /locus_tag="OO339_001303"
     CDS             47838..48569
                     /gene="yehW"
                     /locus_tag="OO339_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5925997.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48550..48657)
                     /locus_tag="OO339_001304"
     CDS             complement(48550..48657)
                     /locus_tag="OO339_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5925998.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(48717..48991)
                     /locus_tag="OO339_001305"
                     /pseudo
     CDS             complement(48717..48991)
                     /locus_tag="OO339_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11811 a        12060 c        13162 g        11958 t
ORIGIN      
        1 cccggcggcg gtccccatca tcacaccggc cagtttctcg ggaagatgca gttcgttgat
       61 aataaatagc ggcatgttga tgatgtacag gctgttcgag ccccacatca atgtacaaat
      121 gacaaacagc agtagcgtat cgcgacggtt acggcgcggc gcttcaaccg tgccggtcgc
      181 cagcggaagc tctttttgca tcgacggtaa aaacagccac accatcacgc cgcaaacaat
      241 aaacgctacc gctgcgctca gatacattac cgtaaagctg aaacccatcg ctaacgcata
      301 agccagcggt gggccaatga cccatgccag tgaaacctga gcgcgtaaaa aagagctgaa
      361 catcaccgcc tcacgtccgg ttttgtcggc atgttcacgg gcaagggcaa acatttgcgg
      421 gttagcggtc gagccaaagc tgctaagaaa gacgccaacg aatagcaaaa caaagtagtt
      481 gcgattccaa gcaaaaaggg tgcaggccag cacgcctaac aggcagcaaa agacaatcag
      541 cgatttgcga tcgccgcgct tatcagagcg cccagcgaga aactgactta ccagaatccc
      601 aatgacagcg ctgcctgtaa agaagaatcc caccatcgcc ggacgggcat gtacttcatc
      661 ggtaaggaaa atactgagtg tcggggtttg cagagcaccc gcaataccgg tgagaaaggc
      721 aacgattaaa aacgccgtcg aggtcaggtc aaacgatttc gcgctggaga ctgcggggga
      781 gttatgcatg ctggtcgatc actggaagag gaagacgcgg agtttacgct gcttgtcaga
      841 aaataaacag acagcgaaat caaaagcgcc acgaataatc aaaatgccat ttcactgttg
      901 ctgattggct gctgacgctg ctgaaatgaa attgctgata acggattttc ccatcagcaa
      961 ttaggaaaaa tgacaaaaaa ttgtgcagca catcaaactt ttgctcataa ctttacggct
     1021 ttccttgcgt gctgaaacag aagtattatg ctttcttgaa acgtttcagc gcgatcttgt
     1081 ctttaaccct aagctaggtt ggcgcttttt ttctcataga ggctgaatcg tttcaattca
     1141 gcaagagagg agaactatgt tccagttatc cgtacaggac atccatccgg gcgaaaaggc
     1201 cggagacaaa gaagaggcga ttcgccaggt cgctgcggcg ctggtgcagg ccggtaatgt
     1261 agcagaaggc tacgtcaatg gcatgctggc gcgcgaacag caaacctcaa cgttcctcgg
     1321 caatggtatt gctattccac acggcactac cgacacccgc gatcaggtgc tgaaaaccgg
     1381 cgttcaggta tttcagttcc cggaaggcgt cacctggggt gacggtcagg tagcgtacgt
     1441 ggcaatcggt attgctgcca gctcggataa acatctgggc ctgctacgcc agctgaccca
     1501 cgtactgagc gatgattccg ttgctgaaca actgaagtca gcaacaacag cagaagaact
     1561 tcgcgcatta ctgatgggcg aaaagcagag tgagcagctg aagctcgaca acgaaatgct
     1621 gacgctggat atcgtcgcca gcgatctgct gactcttcag gcgctgaacg ctgcgcgtct
     1681 gaaagaggcg agggcagttg acgccacttt cgtcaccaaa gccatcaatg aacaaccgct
     1741 gaatctcgga cagggtatct ggctgagcga tagcgccgaa ggcaatctgc gtagtgcgat
     1801 tgcggtaagc cgtgcggcaa atgcttttga tgtggacggc gaaacggcag ccatgctggt
     1861 gagtgtggcg atgaatgacg atcagcccat cgcggttctt aagcgtctcg ctgatttgtt
     1921 gctcgacaat aaagctgacc gcttgctgaa agcggatgcg gcaacgttgc tggcgctgct
     1981 gaccagcgat gatgcgccga ccgacgacgt gttaagcgcg gagtttgtgg tgcgcaatga
     2041 acacggcctg catgctcgtc caggtaccat gctggtcaat accattaaac aatttaacag
     2101 tgatattacc gtgacaaacc ttgatggcac cggcaaaccg gcaaacggac gtagtctgat
     2161 gaaagttgtg gcacttggcg ttaagaaagg tcatcgccta cgctttaccg cccagggtgc
     2221 agatgctgaa caggcgctga aagccattgg cgacgctatc gctgctggtc ttggggaggg
     2281 cgcataatga gcagacgtgt tgctacgatc acccttaatc cggcttatga ccttgttggt
     2341 ttctgcccgg aaattgaacg cggcgaagtg aacctggtga aaaccaccgg tctgcatgcg
     2401 gcgggtaaag gcatcaacgt ggccaaagta ttaaaagacc tgggaattga tgtcaccgtt
     2461 ggcggcttcc tgggtaaaga caatcaggat ggttttcagc aactgttcag cgagctgggc
     2521 attgccaacc gtttccaggt tgtacagggg cgcacccgaa ttaacgttaa gctgacggaa
     2581 aaagacggcg aagtgaccga cttcaacttc tcgggtttcg aagtcacccc cgccgactgg
     2641 gaacgctttg tgactgattc tctgagctgg ctcggtcagt tcgatatggt ctgtgtcagc
     2701 ggaagcttac cgtcaggcgt cagcccggaa gcgttcaccg actggatgac tcgcctgcgt
     2761 agtcagtgtc cttgcattat ctttgatagt agccgtgaag cgttagtagc aggtttgaaa
     2821 gcggcaccgt ggctggtgaa acctaaccgc cgcgagttgg aaatctgggc aggccgtaaa
     2881 ctgcctgaaa tgaaagatgt gattgaagct gcacatgcgc tacgtgaaca aggtattgcg
     2941 catgttgtta tttcactggg tgccgaaggc gcgctttggg ttaatgcctc cggcgaatgg
     3001 atcgctaaac caccgtcagt cgatgtcgta agcaccgttg gcgcagggga ttctatggtt
     3061 ggtggcctga tttatggctt gctgatgcgt gaatccagtg aacacacact gcgtctggcg
     3121 acagctgttg cagccctggc ggtaagtcaa agcaatgtgg gtattaccga tcgtccgcag
     3181 ttggccgcaa tgatggcgcg cgtcgactta caacctttta actgacagca ggagaggcat
     3241 aatgaaaacg ctgctgatta ttgacgctaa tctcggtcag gcacgcgcct atatggcgaa
     3301 gaccctgctg ggcgcggcgg cgcgaaaagc aaaactggaa atcatcgaca atccgaacga
     3361 cgcagaaatg gcgattgttc tcggtgattc catcccgaat gacagcgcgc tgaacggtaa
     3421 aaatgtctgg ctgggtgata tttcccgggc agttgcgcac cctgagctgt tcctgagtga
     3481 agccaaaggc catgcgaaac cttacactgc gccggtcact gcgacagcac cggttgccgc
     3541 cagcggtccg aaacgcgtag ttgcggtgac tgcttgcccg actggcgtag cacacacctt
     3601 tatggcggct gaagccattg aaaccgaagc gaaaaaacgt ggctggtggg tgaaagttga
     3661 aacccgtggt tctgttggcg cgggtaatgc aatcactccc gaggaagtag ccgcagcgga
     3721 tctggtgatt gtggcggcag atatcgaagt ggatctggcg aaatttgctg gtaaaccgat
     3781 gtatcgtacc tctaccggtc tggcgctgaa gaaaacctcg caggaactgg ataaagcggt
     3841 tgctgaagca acgccgtatg aaccggcggg caaagctcaa acggcgacca ctgaaggtaa
     3901 gaaagagagt gcaggcgctt atcgtcactt gctaacgggc gtctcttaca tgctgccgat
     3961 ggtcgttgct ggtggtctgt gtatcgcgct ttcttttgct tttggtatcg aagcgtttaa
     4021 agagccgggt acgttggcag cggcgctgat gcagattggt ggtggttcag cctttgcgct
     4081 gatggtgccg gtactggcag gttatattgc cttctccatt gccgatcgtc cgggcctcac
     4141 gccgggtctg attggcggta tgctggcggt cagcaccggt tctggcttca ttggcggtat
     4201 tattgcgggc ttcctggctg gttacattgc gaagttaatc agtacgcaac tgaaactgcc
     4261 gcagagtatg gaggcgctga aaccgatcct gatcattccg ctaatttcca gtctggtggt
     4321 cggtctggcg atgatctacc tgatcggtaa accagttgct ggcattctcg aagggctgac
     4381 tcactggctg cagaccatgg ggactgcgaa tgcggttctg ctgggggcga tcctcggtgg
     4441 catgatgtgt actgacatgg gcggtccggt aaacaaagca gcgtacgcat tcggtgtggg
     4501 tctgctgagt actcaaacct atggcccgat ggcggcgatt atggcggcag gtatggtgcc
     4561 accgctggca atgggcctgg caacaatggt ggcgcgtcgc aaattcgaca aagcgcagca
     4621 ggaaggtggc aaagccgctc tggtattggg actgtgcttc atttcggaag gtgcaattcc
     4681 gtttgctgct cgtgatccga tgcgtgtgct gccgtgctgt atcgtgggtg gggcgctgac
     4741 tggcgcaatc tcaatggcga ttggtgcgaa actgatggca ccgcacggtg gtctgtttgt
     4801 tctgctgatc cctggcgcta ttacgccggt attgggttac ctggtagcaa ttattgccgg
     4861 tacgctggtg gcgggtttgg cctatgcctt cctgaaacgt ccggaagtgg acgcagtagc
     4921 gaaagcagcg taataaaagg tgtgttacag ggcagaaatt tcctgccctg taacatttga
     4981 tgagcgcaaa gagaatgacc agcttgtgaa tgtaatttta tgaatttaaa tggttttatc
     5041 tttcagtaga aacttcaaac gttcatcaaa cactaaggtt atttcctcaa gggcagatta
     5101 ttttctgccc ttttttagtg ttcctgttct gtgcatcccg tcacaaattc ctcgcttttg
     5161 taattcaggc atttaatcgc attttgtgag cctgctcaaa tctgcccatt ttgcctctct
     5221 ggcggtaatt tactttacgt aaaacatgca tactatgagc acatgtttaa atatgaacac
     5281 atgtttaaac cgtctttgca gtatctatat tcttgtggat tgacagggtt ggtaattttt
     5341 taccagagga cgtgctatgc gcgaaaagga ttatgtcgta attataggtt cggcgaatat
     5401 tgatgtcgcc ggatattcac atgaatcatt aaattatgcg gattcaaatc caggtaaaat
     5461 aaaatttacg cctggtggag tagggcgcaa tattgcacaa aacctggcgt tgctgggtaa
     5521 caaagcctgg ctactgagcg ccgtaggcag tgatttttat ggtcaatcgc tgctaacgca
     5581 aaccaatcaa tctggtgttt atgtcgataa atgcctgatt gtgccgggag aaaatacgtc
     5641 gagttattta tcattactcg ataataccgg tgaaatgctg gttgctataa atgacatgaa
     5701 tattagcaac gctattacag ctgaatatct cgcacagcac cgtgaattta ttcagagggc
     5761 aaaggtcatt gtcgcggact gtaatatcag tgaagaggca ctggcatgga ttctggataa
     5821 tgccgccaac gtacccgtat ttgtcgatcc ggtttcagcg tggaaatgtg tcaaagtgcg
     5881 cgaccgtcta aatcagaacc acactctgaa gccaaaccgc cttgaagcgg aaaccctgag
     5941 tgggattgcg ctgtcagggc gtgacgatgt ggcaaaagtt gctgcctggt tccatcaaca
     6001 tggcctgaac cgactggtat tgagcatggg cggcgacggc gtttattaca gcgatatcag
     6061 cggtgaaaat ggctggtctg cgccgatcaa aaccaatgtt attaatgtta ccggagcggg
     6121 cgatgccatg atggcgggac ttgcttcgtg ttgggtagac ggaatgccgt ttgccgaatc
     6181 tgttcgtttc gcacagggat gttcgtcaat ggcgctctcc tgtgaataca ccaataaccc
     6241 cgatttatcg attgccaacg ttatatcgtt agtggagaac gcagaatgtc tgaattaaaa
     6301 atttcccctg aattattaca aatttctccg gaagtgcagg acgctttaaa aaacaaaaaa
     6361 ccggttgtgg cgctggaatc gaccattatt tctcacggga tgccgttccc acaaaatgcc
     6421 cagaccgcaa ttgaagtaga agaaactatt cgtaaacagg gcgcagtacc tgccactatc
     6481 gccattattg gcggcgtgat gaaagtgggt ttaagcaaag aaaaaattga attactgggt
     6541 cgtgaagggc ataacgtgac caaagttagt cgtcgcgatt taccttttgt tgttgccgcc
     6601 ggaaaaaatg gcgcaaccac tgtggcttca acgatgatta ttgcggcgct tgccggaatt
     6661 aaagtatttg ccaccggggg aattggtggt gtgcatcgcg gggcggaaca taccttcgat
     6721 atttctgccg atttgcaaga actggcaaat actaatgtca ccgttgtttg tgccggggcg
     6781 aaatctattc tcgatttagg attaaccact gagtatttag aaaccttcgg tgtgccgtta
     6841 attggctatc agactaaagc gctgcctgcg tttttctgcc gtaccagctc gtttgacgtc
     6901 agcattcgtc tcgacagcgc cagtgaaatt gcccgtgcaa tggcggtgaa atggcaaagc
     6961 gggctgaacg gtggcctcgt ggtagcgaac ccgatcccgg aacagtttgc gatgccggag
     7021 gaatctatca atgcagccat agatcaagcc gtcgccgaag ccgaagagca gggcgttatt
     7081 ggtaaagaaa gtacaccgtt cctgctggcg cgcgttgctg agctgaccag cggtgacagc
     7141 ctgaaatcca atatccagct ggtgttcaac aacgccattc tggcgagtga aattgccaaa
     7201 gaataccagc gtctcgcggg ttaatttctg acgggcagaa agccgtcgcc cgtactcatc
     7261 aagcatacca aaacctggcg ctgacgccgg gtttactggc atgaagggaa aatcattatg
     7321 gatataatga gaagtgttgt ggggatggtg gtgttactgg caatagcatt tctgttgtca
     7381 gtgaataaaa agagcatcag tttgcgcacg gttggagccg cactgctgct gcaaatcgct
     7441 attggtggca tcatgctcta cttcccaccg ggaaaatggg cagtagaaca ggcggcatta
     7501 ggcgttcata aagtgatgtc ttacagtgat gccggtagcg ccttcatttt tggttcgctg
     7561 gttgggccga aaatggatgt cctgtttgac ggtgcgggtt ttatcttcgc ctttcgcgta
     7621 cttccggcga ttattttcgt tactgcgctc atcagtctgc tgtactacat tggcgtgatg
     7681 gggctgctga ttcgcatcct tggcagcatt ttccagaaag ccctcaacat cagcaaaatc
     7741 gaatcttttg ttgcggttac cactattttc ctcgggcaaa atgagatccc ggcgatcgtt
     7801 aaaccgttta tcgatcgcgt gaatcgcaac gagttgttta ccgcaatttg tagcgggatg
     7861 gcgtccattg ctggttcggt gatgattggt tatgccggaa tgggcgtacc aattgactac
     7921 ctgttagcgg catcgctgat ggcgatcccc ggcggtattt tgtttgcacg tattcttagc
     7981 ccggcaaccg agccttcgca ggtcacattt gaaaatctgt cgttcagcga aacgccgcca
     8041 aaaagcttta tcgaagcggc ggcgagcggt gcgatgaccg ggctgaaaat cgccgctggt
     8101 gtagcgacgg tggtaatggc gtttgtcgca attattgcgc tgatcaacgg cattatcggc
     8161 ggaattggcg gctggtttgg tttcgccaat gcctctctgg aaagtatttt tggctatgtg
     8221 ctggcaccgc tggcgtggat catgggtgtg gactggagtg atgccaacct tgctggtagc
     8281 ctgattgggc agaaactggc gattaacgaa ttcgtcgctt acctgagttt ctccccatac
     8341 ctgcaaacgg gcggcacgct ggaagtgaaa accgtggcga ttatatcctt tgcgctttgt
     8401 ggttttgcta actttggttc tatcggtgtt gtcgttggcg cattttcggc tatttcgcca
     8461 aaacgtgctc cagaaatcgc ccagcttggt ttacgggcgc tggcagcagc aacgctttcc
     8521 aacctgatga gtgcgactat tgccgggttc tttattggtc tggcgtaatg ctcgatttgt
     8581 agaccggata aaacgtatta agcgttgcat ccggcaatcg cgctattgca aataattttt
     8641 actgcatcat cccggagaat ttattctccg ggtccatctc cagcgccagt cctgacaact
     8701 gctttctgtt tttaattaga tatcctttct tttaattaat aaaccgtcgt gaatgaattg
     8761 cgcgagaaca tataaaagat gtcgataaga aacgcctaaa tactctgccg cttgcgtatg
     8821 cttttcgtga taaagatcac cttcttgtga gagtaatata aatgctgcca ggcgattcac
     8881 taacggaaat gattgattct gagttaaaga aacaatatta cgataatttt tatgacttaa
     8941 ggtgacgcaa agttttcgta aaaatagggt gtcgtttaat aacagcggac ggtaatgttt
     9001 catagggagc gcaaggcacc aacactcttc aatagcctgc accgcacgcg gttcatggtc
     9061 tttgtcgatt aactcaatct cgccaataaa acagggagcg gcaaagaaat cgatcagcga
     9121 cacgcgacca ttggcaagcg tggcgtaaag cctggcgcgg cctcgcgtca gataaaacag
     9181 ccatgacggc tgttgccctt cctgcacaat gtagtcacgc gctaaaaaat gaaacagccg
     9241 cgtatcggct gaaacatccg ttgaaaagca atccttaaac gcagattcac tcatcaattg
     9301 ctgcttaagg tcattattgt ggatttcttt cacgatgttt atccgctatg agatttctca
     9361 tatttagcat acgtacgctg ttgttaagat tcaatcattg gcccggtcga attttctttg
     9421 ctgtagaaaa aaataacgca actggaaaca gaggaaataa acaatggaaa agagaaaaat
     9481 tattctggat tgtgagccgg gtcatgatga tgctattgca atgatgatgg cagcgaaaca
     9541 tccggcaata gatttattag gcatcactat tgtagcgggt aatcagacgc ttgataaaac
     9601 attaattaat ggcctgaatg tttgccagaa actggagatt aatgttccgg tttatgcggg
     9661 gatgccgcaa cccattatgc gtaaacaaat cgttgccgat aatattcacg gtgaaaccgg
     9721 actggatggc ccggtattcg agccgctgac ccgccaggca gaaagtaccc atgcggtgaa
     9781 atatatcatc gataccctga tggcaagcga tggcgatatc actctggtgc cggttggtcc
     9841 gctttcaaat atcgcggtgg caatgcgtat gcaacccgca atcctgccca aaatccgtga
     9901 aattgtgcta atgggcggcg cttacggtac aggcaacttc accccatctg ccgagttcaa
     9961 tatctttgcc gacccggaag ccgcccgcgt ggtgttcacc tccggcgttc cattagtgat
    10021 gatgggcctc gatcttacca accagaccgt ttgcaccccg gatgtaattg ctcggatgga
    10081 aagggtaggc ggccctgccg gagagctgtt cagcgacatc atgaacttca ctctcaaaac
    10141 gcagttcgaa tactacggcc tggctggcgg cccggtgcat gacgccacct gcattggtta
    10201 tctgatcaac cctgatggca ttaaaaccca ggatatgtac gtcgaagtgg acgtcaacag
    10261 cggcccttgc tacgggcgta cggtctgcga cgaactgggc gttcttggca agcccgctaa
    10321 taccaaagtc ggcatcacta ttgataccga ctggttctgg ggcttagtcg aagagtgcgt
    10381 gcgcggctac atcaaaaccc attaagtgtt gataacaagc cgggacagta tcgcaagatg
    10441 caggcccggc tatttctgat gggcagaaag ccgtcgccca aaatgacatg aagagataat
    10501 cactatggat gtcatgagaa gtgttctggg aatggtggta ctgctggcaa ttgcgttttt
    10561 actgtcagta aacaagaaga agatcagcct gcgtaccgtt ggcgcggcgt tagtgttaca
    10621 ggtcgtgatt ggcgggatta tgctttggtt accgccaggg cgttgggtcg cagaaaaagt
    10681 cgcttttggc gtccacaaag tgatggcgta cagcgacgca ggtagcgcat ttatcttcgg
    10741 ttctctggtc ggaccgaaaa tggatacctt atttgatggc gcaggattta tctttggttt
    10801 cagggtatta ccggcaatta tcttcgtcac tgcactggtg agtattctct actacatcgg
    10861 tgtgatgggg atcttaattc gcattctcgg cggtatcttc cagaaagctt taaatatcag
    10921 caagatcgag tcattcgtcg cggtcaccac cattttcctc gggcaaaacg aaattccggc
    10981 gatcgtcaaa ccctttatcg atcgtctgaa tcgcaatgaa ttatttacag cgatttgtag
    11041 tggcatggcc tcgattgctg gttcgacaat gattggttac gccgcactgg gcgtacctgt
    11101 ggaatatttg ctggcggcat cgttaatggc gatccctggc gggatcttgt ttgcccgcct
    11161 gttaagcccg gctacggaat cttcgcaggt ttcttttaat aacctctctt tcaccgaaac
    11221 accgccaaaa agcattattg aagccgatgc gacaggggca atgaccgggc tgaaaatcgc
    11281 cgccggtgta gcgacagttg ttatggcatt tgtcgccatc attgcgttaa ttaacggtat
    11341 tatcggcggc gttggcggct ggtttggttt tgaacatgcc tcgctggagt ccattttagg
    11401 ttacctgctg gccccacttg cgtgggtaat gggtgttgac tggagtgatg caaatcttgc
    11461 cgggagtttg attggacaga aactggcaat aaatgaattt gtcgcttatc tcaatttctc
    11521 accctatctg caaacggctg gcactctgga tgctaaaacc gtggcgatta tttccttcgc
    11581 gttgtgcggt ttcgctaact ttggttctat cggggtggtg gtgggggcgt tttctgcggt
    11641 tgcgccacac cgtgcgccgg aaatcgccca gcttggttta cgcgcgctgg cggcggcgac
    11701 actttctaat ctgatgagtg ccaccattgc cgggttcttt attggtttag cgtagtgtgc
    11761 aatttatgag ccggattaga tgcggtcaag cgtcgaatcc ggcaacattg ttacaccatt
    11821 ggcactaatg acagcgcgtt atcggcagac agggtagggt tgtttaagct gccgctggcg
    11881 cgtgaaattg ccgcgcaggc catcgcaaaa cgggcgctgt cacggaaatt gtttccctcc
    11941 agaaagctat ataccagacc cgccataaaa ccatcgtcag caccaaaact gtctaccgtc
    12001 gtatgcgctg gcacagtcag caaaaattgt tcaccatcct tttcgctgca ataaactgac
    12061 tcatcgggca aataaacaaa cagttgctga acaccttgct gatgcaatgc attcactgcg
    12121 gcattacggt cagcatcgct ggtgatcgcc tgtccccata aaatttccag ctccggtaaa
    12181 gtgggtttca gggtgtgaat atgcgccagc cagtgtttga ttttgcccgc tttgaattct
    12241 gaaacggtat cgacaaacac cgggatctca tcagcgaggg taaagaccca ttccagcgcc
    12301 tcggctgtca ggttgcaatc agccagtacc acgcccgcat gacgaagtaa atcgcgcgac
    12361 ccgttcagta gttgcggtgt caactgttcc agcagatggg tatcgttaat tgccagcacg
    12421 gtttgatcgt ctcgattggc aattgccaga tacgtcgatg tgctttgacc atgcaaacga
    12481 acgcagccgg agacattcac gccggcacgg cgcgtttctt ccaggagcat ttcgccataa
    12541 aagtcatcgc caatcactga aagcaaatgg acgtcacggc ctaacagcgc cagattgtgg
    12601 gcgatattgc gtccaacgcc gcctgccgaa caatgaattg taccgggatg agaagccgct
    12661 tgcgggtaat ggatatccgc catcccgcga atatccatat tgattgtccc caccactacg
    12721 cagtattcct gctcggtgag aatgtaacct ttgtctttaa tccggccttt gcgcattaaa
    12781 tccataatat gcgccgcaac gcgcgaacga ctgatttgca gcatgtccgc aatttcgttc
    12841 tgctgaatca gcgggttacg ccgtaaaatt gcaaggatct ccttttcccg gttatttatc
    12901 ttcaggctac cgctttttca gtttgttgcg ctttcagcca ggcgatctct tctgcccaga
    12961 tatccgggtt gatggtttca aggatcagcg gaatgccgtc gaaacggtcg ttctgcataa
    13021 tccagcggaa cgcatcatga ccgatattgc cttcaccgag gctatgatgg cggtcaacgc
    13081 ggctgccaaa ggtacttttc gcatcgttaa ggtgcatccc gcgcaaatac ttaaagccga
    13141 caatacgggc aaaatcagcg aacgtttttt cgcattcggc tggagtacgc aaatcatacc
    13201 cggcagcgaa agcatggcag gtatcaatgc agacgccgac gcgggattta tcttccacgc
    13261 cgtcgataat cgccgcgaga tgttcgaatt taaaccctaa gttactgccc tgaccggcgg
    13321 tgttttcaat caccgctgtt acaccttgag ttttatccag cgcaatgttg atggattcgg
    13381 caatacgcgc aaggcaatcc tcttctgaaa tctgcatcag atggctgcca gggtggaagt
    13441 tgagcaaaga aagccccagt tgttcgcaac gctgcatttc atctataaag gcatcgcgcg
    13501 atttttccag agcttcagtg accggatgtc cgaggttgat cagataactg tcgtggggaa
    13561 gaatttgcgc cgatgtgtag tgatattttt cacaggcggc tttgaattca tcgatggttt
    13621 gcgtcgtgag cggtgcggca cgccactgac gttggttttt ggtgaacaag gcaaacgcgg
    13681 ttgcgtcgat ttcggcggcg cgaattgcgg catttgccag accgccagca gcactaacgt
    13741 gcgctccaat gtatttcatg cgaggactcc tgttaaaccc gctggaggaa aacggtaatg
    13801 atagcgggtt aacaggagca agaagtagtg gtttatgcga tgacgctttg aatcacatag
    13861 ttaatcgcac caccaccaac aatcagccag gcaaacagta ccagtgccat cagcagaggt
    13921 ttcgccccag cttttttcag cgcgctgacg tgagtggtca gacccagcgc cgccatcgcc
    13981 attgccagca ggaaggtatc cagcgttacc agcatgttca ccacgctctg cggtaacagg
    14041 tggaacgagt taaagatggc aactacgatg aacaagatgg caaaccacgg aatagtgatt
    14101 ttgcttttct cgccgctgtt cgccccagac agctgtttaa cacgcgccgc cagcaggatg
    14161 aggaacggag ccagcatcat cacgcgcagc attttggaaa taactgctgc gttttctgca
    14221 tccgggctga tggcatgacc tgccgccacc acctgcgcca cttcgtgcac agtagaacca
    14281 atgtagatac cgaaagtttc cggactaaac cattgagaca tcagcggata tatcgccggg
    14341 tagaggaaaa tcgcgacggt cccgaagata acaacggttg caacagccac ggttacttta
    14401 ctggcttccg ctttcactac cggctcagtc gccagtaccg cggcagcacc acagatactg
    14461 ctaccggcac cgatcaacca actggtgtgc ttatccagac caaacacttt ctgccccagg
    14521 aagcaagcca gcaggaaggt actggacagc gtcagcacgt caatgatgat cccactgata
    14581 ccgacatcgg caatttgcga gaacgtcaga cggaagccat aaagaatgat acccagacgt
    14641 aataaatatt gcttggcaaa cagcacacca ccgtcacagc ttttccagat gtgcggatag
    14701 atggtgttgc ctaaaaccat ccccaacaag attgcgaggg tgagggcact aaacccggca
    14761 cccgcaaccg cgggaatgga accaccccac agggcgaccc cggtgataac tgcactcagg
    14821 gctaaccccg gaataaaatg ccacagtgta cgatgttgtt tctgtaaggt gatattcgtc
    14881 ataaccctct cctttaaccg ggcataaggt tacgactaac tggtttaaaa ataaaattga
    14941 ttatatattt ataattaatc tttataagtg gtaagcgact atgcacatca ccctccggca
    15001 gttggaagtt tttgcagaag tattgaaaag tggatcaacc acccaggcgt cggtgatgct
    15061 ggcgttgtcg caatcagcag tgagcgcagc cttgactgac ctggaagggc agcttggcgt
    15121 tcaactgttt gatcgcgtgg ggaaaagact ggttgttaat gaacacgggc ggctgctcta
    15181 tccgcgagcg ttggcactgc ttgaacaggc ggttgaaatc gaacaactgt ttcgcgaaga
    15241 caacggcgcg attcgtatct atgccagtag taccatcggt aactacattc tgcctgcagt
    15301 tatcgcccgt tatcgccatg attatccgca gttgccgatt gaacttagcg ttgggaacag
    15361 ccaggacgtg atgcaagcgg tgctggattt ccgcgttgat attggcttta ttgaaggacc
    15421 gtgccacagc actgaaatca tttctgaacc gtggctggaa gacgagctgg tggttttcgc
    15481 cgcgccgact tcgccgttgg ctcgtggtcc ggtcacctta gaacagctgg ccgctgcgcc
    15541 gtggatcctg cgtgaacgcg gttccggcac gcgggatatt gtcgattatc tgttgctgtc
    15601 acatttaccg aagtttgaga tggcgatgga attaggtaac tccgaggcaa tcaaacatgc
    15661 ggtgcgtcat gggttgggaa ttagttgcct gtcgcgacgt gtgattgaag atcaattgca
    15721 ggcaggcaca ttaagtgaag ttgcggtccc tctgccgcgc ctgatgcgta cgttgtggcg
    15781 tatacatcat cggcaaaaac acctttccaa cgcgctacgg cgctttctgg actattgcga
    15841 tcccgcaaat gtgccgcgtt aagttgctgt acaagaacat gctggtgctg tgtcgatttc
    15901 gtgacgcagc gccttcagca tgcattcgcc agaaaagaga ttggctgctt tacttataat
    15961 ccctgggcga tcatgaaggt gtcttataac cgtgtatttc tgccggaagg attgccaatc
    16021 gtctgctaca atcgcgccat catttttaag atggatagca tttttgtatg ggttccgaaa
    16081 ctaaaactac agaagcgccg ggcttacgcc gtgaattaaa ggcgcgtcac ctgacgatga
    16141 ttgccattgg cggttccatc ggtacaggtc tttttgttgc ctctggcgca acgatttctc
    16201 aggcaggtcc gggcggggca ttgctctcgt atatgctgat tggcctgatg gtttacttcc
    16261 tgatgaccag tctcggtgaa ttggctgcat atatgccggt ttccggttcg tttgccactt
    16321 acggtcagaa ctatgttgaa gaaggctttg gcttcgcgct gggctggaac tactggtaca
    16381 actgggcggt gactattgcc gttgacctgg ttgcagcaca gctggtcatg agctggtggt
    16441 tcccggatac accgggctgg atctggagtg cgttgttcct cggcgttatc ttcctgttga
    16501 actacatctc tgttcgcggc tttggtgaag cggaatactg gttctcactg atcaaagtca
    16561 cgacagttat tgtctttatc atcgttggtg tgctgatgat tatcggtatc ttcaaaggcg
    16621 cgcagcctgc gggctggagc aactggacaa tcggcgaagc gccgtttgct ggtggttttg
    16681 cggcgatgat cggcgtagct atgattgtcg gcttctcttt ccagggaacc gagctgatcg
    16741 gtattgctgc aggcgagtcc gaagatccgg cgaaaaacat tccacgcgcg gtacgtcagg
    16801 tattctggcg aatcctgttg ttctatgtgt tcgcgatcct gattatcagc ctgattattc
    16861 cgtataccga tccgagcctg ctgcgtaacg atgttaaaga catcagcgtc agtccgttca
    16921 ccctggtgtt ccagcacgcg ggtctgctct ctgcggcggc ggtgatgaac gcagttattc
    16981 tgacggcggt gctgtcagca ggtaactccg gtatgtatgc gtctactcgt atgctgtaca
    17041 ccctggcgtg tgacggtaaa gcgccgcgca ttttcgctaa attgtcgcgt ggtggcgtgc
    17101 cgcgtaatgc gctgtatgcg acgacggtga ttgccggttt gtgcttcctg acctccatgt
    17161 ttggcaacca gacggtatac ctgtggctgc tgaacacctc cgggatgacg ggttttatcg
    17221 cctggctggg gattgccatt agccactatc gcttccgtcg cggttacgta ttgcagggac
    17281 acgacattaa cgatctgccg taccgttcag gtttcttccc actggggccg atcttcgcat
    17341 tcattctgtg tctgattatc actttgggcc agaactacga agcgttcctg aaagatacca
    17401 ttgactgggg cggcgtagcg gcaacgtata ttggtatccc gctgttcctg attatttggt
    17461 tcggctacaa gctgattaaa ggaactcact tcgtacgcta cagcgaaatg aagttcccgc
    17521 agaacgataa gaaataagtt tcctcccttc cttgctaagc cctctcaacc gagagggctt
    17581 tttcaattcc atttccctga caaatcatgc ggatataaaa tttaacattt ggattgataa
    17641 ttgttatcgt ttgcattatc gttacgacgc aatcaaaaaa ggctgacaaa tcagaggctg
    17701 ttccggcttt ctgggatgat cacctgcata caaataagtc caccgcgatg ctgccgtacg
    17761 caaggggacg taaagaagat gtgagcgata cccattttat tttcgtagtt acctcatgga
    17821 gatatggaat gtttaggttg aaccctttcg tacgggtcgg gctgtgtttg tccgctattt
    17881 cttgtgcatg gcctgtgtta gcggtcgatg atgatggcga aacgatggtt gtcactgcat
    17941 cttccgtgga acaaaatctt aaagatgcac ccgccagtat cagcgtcatt acccaggaag
    18001 acctgcagcg aaaaccggta cagaatctga aggatgtcct caaagaagtg cctggcgtac
    18061 aactgacgaa cgaaggggat aaccgtaagg gcgttagtat tcgtggtctg gacagcagct
    18121 acaccctgat tcttgtcgac ggtaaacgcg ttaactcccg caatgccgtc ttccgccaca
    18181 atgatttcga tctgaactgg atcccggtcg attccatcga acgtattgaa gtggttcgtg
    18241 gcccgatgtc gtcgctatac ggctccgatg cgctcggcgg tgtagtgaat atcatcacca
    18301 aaaaaatcgg tcagaaatgg tcgggtaccg ttaccgtcga taccaccatt caggaacatc
    18361 gcgatcgcgg tgatacctat aacggtcagt tctttaccag tggaccatta attgatggtg
    18421 tgctgggaat gaaagcttac ggcagcctgg caaaacgtga aaaggatgac ccgcaaaact
    18481 caacgaccac cgataccgga gaaacgccgc gtattgaagg attctccagc cgcgacggca
    18541 atgtcgaatt tgcctagaca ccgaatcaaa atcacgattt tactgccgga tacggtttcg
    18601 accgtcagga tcgtgattcc gattcgctgg acaaaaaccg cctggaacgc cagaactact
    18661 gcgtcagcca taatgggcgt tgggattacg gcaccagcga actgaaatcc tacggtgaga
    18721 aagtcgagaa caaaaaccct ggcaacagca gcccgataac ttccgaaagc aatacggtcg
    18781 acggcaaata cacgttgccg ctgacggcga ttaatcagtt tctcacggtt ggcggtgaat
    18841 ggcgtcacga caaacttagc gatgcggtga acctgaccgg gggaaccagc tccaaaacgt
    18901 ctgccagcca gtacgcgctg tttgtggaag atgaatggcg gatcttcgag ccgctggcgc
    18961 tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg tgaacactgg agtccgcgtg
    19021 cctacatggt ttataacgcc accgacaccg taacggtgaa agggggctgg gcgacggcat
    19081 ttaaagcacc ttctctgttg caacttagcc ctgactggac gagcaattcc tgccgtggcg
    19141 catgtaagat tgtgggtagc ccggatctga aaccagaaac cagcgaaagt tgggagctgg
    19201 ggctttacta catgggtgaa gaaggctggc tggaaggggg tgaatccagc gttatcgttt
    19261 tccgtaacga tgtgaaagat cgtatcagca tcagccgtac gtctgacgtc aacgctgcac
    19321 cgggctacca aaactttgtt ggttttgaga cgggcgctaa cggacggcgc ataccggtat
    19381 ttagctacta cagcgttaac aaagctcgta ttcagggcgt ggaaaccgaa ctgaaaattc
    19441 cgttcaacga tgaatggaaa ctgtcgatca actacaccta caacgatggt cgtgatgtca
    19501 gcaacggcga aaacaaaccg ctatccgatc tgccgttcca tactgctaac ggtacgctgg
    19561 actggaaacc gctggcgctg gaagactggt cattctatgt ttctggtcac tataccgggc
    19621 agaaacgcgc cgacagcgcg acggctaaaa caccgggcgg ttataccatc tggaataccg
    19681 gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc aggcgtgctg aaccttggcg
    19741 acaaggatct cagtcgtgac gactacagct ataacgaaga cggacgtcgt tactttatgg
    19801 cagtggatta tcgcttctga tgagaagatg cccggcgaac cgggcggact ttcacttcag
    19861 taaatactgc gcatggaagc gcaggtgatc ctctataaaa gaggcgatga agtagtaact
    19921 gtgatcatat cccggctgaa tacgcagcgt catcggccac gctttctggc gcgcggcttc
    19981 tgccaatacc gcaggttgca actggtcggc aagaaactga tcattatcgc cctgatcgat
    20041 aagcgtcggg atcgcatcct gcgcgttact ggcatacatc agcgcgcaac tatcccattc
    20101 cagcatgcat ttttgtcctc acctaaatag gtgctaaacg ctttgattcc ccacgggacg
    20161 ctgcacggat tcacaatggg cgcaaaggcc gaaacgctgg tgtatttacc cggatttttc
    20221 agcgccataa tcagcgcacc gtgaccaccc attgagtgac cgctaatggc gcagcggtcg
    20281 ctgacattaa attgcgactg aaccagcgcc ggtaattcat cgcgcagata atcatacatc
    20341 cggtaatgcg tcgcccacgg cggttgcgtg gcattaagat aaaagcctgc gccctggcct
    20401 aaatcgtagc catcgtcgtt ggcaaccttt tcgccgcgcg ggctggtgtc tggcatcacc
    20461 agtacaatcc ccagttccgc cgctacccgc tgggcacccg ccttggtggt gaagttctcg
    20521 tcattgcagg ttaatccgga aagccagtac agcactggtg gcggggtgtg atcacgaggg
    20581 ggggggagaa agatactgaa cgtcatcggg cagtttaagg tactggagtc gtgtcgccag
    20641 cgttgctgcc agccttcaaa acagcggtgc tcttcgagca tttccatgcg tggctccttg
    20701 ttgtgttgtt tgcaatttct tcatcatacc gtttattcaa tgtgctgtga gtaactttca
    20761 cttccgtatt tgcataacga tgttttaaca tctgctgatg aaaggcagcg gcaattacaa
    20821 taattatcgc tgtgaatact ggattatgtg cgccgcctca cgcacaataa tcaagctgta
    20881 aatcagctta ataactttgc ccccacgcag ggtggaggcg tcacacctgc aggagaaatc
    20941 ataaatgcca tcactcagta aagaagcggc cctggttcat gaagcgttag ttgcgagagg
    21001 actggaaaca ccgctgcgcc cgcccgtgca tgaaatggat aacgaaacgc gcaaaagcct
    21061 tattgctggt catatgaccg aaatcatgca gctgctgaat ctcgacctgg ctgatgacag
    21121 tttgatggaa acgccgcatc gcatcgctaa aatgtatgtc gatgaaattt tctccggtct
    21181 ggattacgcc aatttcccga aaatcaccct cattgaaaac aaaatgaagg tcgatgaaat
    21241 ggtcaccgtg cgcgatatca ctctgaccag tacctgtgaa caccattttg ttaccatcga
    21301 tggcaaagcg acggtggctt atatcccgaa agattcggtg atcggtctgt caaaaattaa
    21361 ccgtatcgtg cagttctttg cccagcgtcc gcaggtgcag gaacgtctga cgcagcaaat
    21421 tcttattgcg ctacaaacgc tgctgggcac caataacgtg gctgtctcga tcgacgcggt
    21481 gcattactgc gtgaaggcgc gtggcatccg cgatgcaacc agtgccacga caacgacctc
    21541 tcttggtgga ttgttcaaat ccagtcagaa tacgcgccac gagtttctgc gcgctgtgcg
    21601 ccatcacaac tgattaaaag gcaggaacca tggagcgcaa cgtcacgctc gattttgttc
    21661 gcggcgtcgc cattctgggg atcctgctat taaacatcag cgcctttggg ctaccaaagg
    21721 cggcttatct caatcccgcc tggtacgatg ctattacgcc gcaggatgca tggacctggg
    21781 catttctcga tctcatcggc caggtgaaat tcctcacgct ttttgcgctg ctgtttggtg
    21841 ctggcctgca aatgttgctg ccccgtggca gacgctggat ccagtcgcgg ttaacgctgt
    21901 tagtcttgtt gggctttatt cacggtttat tgttctggga cggcgatatt ctgctggctt
    21961 acgggctggt ggggcttggc gttttgctgt tattggggct gatttccgac agccagacca
    22021 gccgcgcctg gacgccggat gcatcggcta ttttgtatga aaaatactgg aagcttcacg
    22081 gcggcgttga agcgatcagt aatcgtgccg atggtgttgg caacagttta ctggcactgg
    22141 gcgcacagta tggctggcaa ctggcaggga tgatgctcat tggtgcggcg ctgatgcgca
    22201 gcggctggct gaaagggcag ttcagcttac gtcactatcg tcgtactggt tttgtgctgg
    22261 tggcgattgg ggtgaccatt aaccttcctg ccatcgccct gcaatggcag ctggactggg
    22321 cgtatcgctg gtgtgccttc ttacttcaaa tgccgcggga actgagtgcg ccgtttcagg
    22381 cgattggcta tgcgtcgctg ttttatggct tctggccgca attgagccgc tttaagctgg
    22441 tgcttgcgat cgcctgcgtc ggacggatgg cgctgaccaa ctatctattg caaacgctga
    22501 tttgtaccac gcttttttac cacctcggtt tgtttatgca ttttgaccgc ctggagctgc
    22561 tggcgtttgt tattccggta tggctggcga atatcctctt ctctgttatc tggctgcgtt
    22621 acttccgcca ggggccggtg gaatggctct ggcgtcagtt aactttgcgt gctgccggac
    22681 cggcaatatc taaaacatca agataacgat ctggattaca ttcgttaaca aaacggctgt
    22741 aaccgtttcc attgctgtga ctcgattcac gaagtcctgt attcagtgct gacaaaatag
    22801 ccgccagcaa gcagtcattt actgcaatct cataacaggt agtgaatatg atcaccattc
    22861 gtgatgtagc gcgtcaggct ggcgtctctg tggcaacggt ttcccgggtg ctcaataaca
    22921 gcacgctggt cagtgccgac acgcgtgaag cagtaatgaa agccgtgagt gagctggatt
    22981 atcggccaaa cgccaatgcc caggcgctgg caactcaggt tagcgacacc attggcgtgg
    23041 tggtgatgga cgtttctgat gcgtttttcg gcgcgctggt aaaagcggtg gatctggtcg
    23101 cccagcagca tcagaaatac gtgctaatcg gcaatagcta tcatgaagcg gaaaaagagc
    23161 gtcacgccat tgaggtgtta attcgccagc gttgtaatgc gttgattgtt cactcaaaag
    23221 cattgagtga cgatgaactg gcgcaattta tggataacat tcccggtatg gtgttaatca
    23281 accgcgttgt gccggggtac gcccatcgtt gcgtttgcct ggataatctc agcggtgccc
    23341 gaatggcgac gcgcatgttg ctgaataacg gtcatcaacg tattggttat ctttcttcca
    23401 gccacggcat tgaagatgac gccatgcgta aagcaggctg gatgagtgcg ttgaaagagc
    23461 aggatattat tccgccggaa agctggattg gcactggtac gccggacatg ccgggcggtg
    23521 aggcggcgat ggttgaactg ctggggcgca atctacaact taccgctgta tttgcttata
    23581 acgacaatat ggccgctggc gcactgacag cattaaaaga taatggcatt gcgattccgt
    23641 tacatctctc aatcatcggt ttcgatgata ttcccatcgc ccgttacacc gacccgcaat
    23701 taacgaccgt gcgttatccc attgcttcaa tggctaaatt agccaccgaa ctggccttgc
    23761 agggggcagc aggcaatatt gatcctcgtg ccagccactg ttttatgccg acgttagtgc
    23821 gtcgccattc tgtcgcaacg cgccagaatg cggcggcgat cactaactca acaaatcagg
    23881 cgatgtaacc gctttcaatc tgtgagtgat ttcacagtat cttaacaatg tgatagctat
    23941 gattgcaccg ttttaatgtt gtaacccgta tgtaacagtg aataatcact ttcaccgagg
    24001 taatagcgtc ataacaacaa ttaaagccgt tttctggagc gttaccgggc atggaagaac
    24061 gaattttaaa aagtgagctt cggcgttcag taacacttca ttaactctac tgccccgccg
    24121 agcatttatc tcaagcacta ccctgcataa gaaaaaccgg agataccatg aataagaagg
    24181 tgttaaccct gtctgctgtg atggccagca tgttattcgg tgccgctgca cacgctgctg
    24241 atactcgcat tggtgtaaca atctataagt acgacgataa ctttatgtct gtagtgcgca
    24301 aggctattga gcaagatgcg aaagccgcgc cagatgttca gctgctgatg aatgattctc
    24361 agaatgacca gtccaagcag aacgatcaga tcgacgtatt gctggcgaaa ggggtgaagg
    24421 cactggcaat caacctggtt gacccggcag ctgcgggtac ggtgattgag aaagcgcgtg
    24481 ggcaaaacgt gccggtggtt ttcttcaaca aagaaccgtc tcgtaaggcg ctggatagct
    24541 acgacaaagc ctactacgtt ggcactgact ccaaagagtc cggcattatt cagggcgatt
    24601 tgattgctaa acactgggcg gcgaatcagg gttgggatct gaacaaagac ggtcagattc
    24661 agttcgtact gctgaaaggt gaaccgggcc atccggatgc agaagcacgt accacttacg
    24721 tgattaaaga attgaacgat aaaggcatca aaactgaaca gttacagtta gataccgcaa
    24781 tgtgggacac cgctcaggcg aaagataaga tggacgcctg gctgtctggc ccgaacgcca
    24841 acaaaatcga agtggttatc gccaacaacg atgcgatggc aatgggcgcg gtagaagcac
    24901 tgaaagcaca caacaagtcc agcattccgg tgtttggcgt cgatgcgctg ccagaagcgc
    24961 tggcgctggt gaaatccggt gcactggcgg gcacagtact gaacgatgct aacaaccagg
    25021 cgaaagcgac ctttgatctg gcgaaaaacc tggccgatgg caaaggtgcg gctgatggca
    25081 ccaactggaa aatcgacaac aaagtggtcc gcgtacctta tgttggcgta gataaagaca
    25141 acttggctga attcagcaag aaataagact gattgggcgc agtcgtttac tgcgccctta
    25201 ttgtatggcc aacaaggtaa aattatatgg tcagctcaac gactccgtct tccggggaat
    25261 acttgttgga aatgagcggt atcaacaagt cctttcctgg tgttaaggca cttgataacg
    25321 ttaatttaaa agtccggcca cattctatcc atgcattaat gggggaaaac ggtgcaggaa
    25381 aatcgacatt attaaaatgc ctgtttggta tttatcaaaa agactccggc accattttat
    25441 tccagggtaa agagatcgat ttccattccg ccaaagaggc actggaaaat ggtatttcga
    25501 tggtacacca ggagttaaac ctggtattac aacgttcggt gatggataac atgtggctgg
    25561 ggcgatatcc caccaaaggc atgtttgtcg atcaggacaa aatgtaccgc gaaaccaaag
    25621 cgatttttga tgaactggat attgatatcg atccgcgtgc gcgcgtcggc acattatccg
    25681 tttcgcaaat gcagatgatc gaaatcgcca aagcgttttc ctataacgcg aaaattgtga
    25741 ttatggatga accgacttct tcgttaaccg aaaaagaggt caatcatctg ttcactatta
    25801 ttcgtaaatt aaaagagcgc ggctgcggta ttgtttatat ctcgcataaa atggaagaaa
    25861 tcttccagtt atgtgatgaa gttaccgtat tgcgcgacgg tcagtggatc gccaccgaac
    25921 cgctggcagg actgacgatg gacaagatca tcgccatgat ggttgggcgt tctcttaatc
    25981 agcgttttcc tgacaaagaa aacaagccgg gcgaagtcat cctcgaggtg cgtaacctaa
    26041 cgtcactgcg ccagccgtcg attcgcgatg tctcgtttga tctgcataaa ggggagatcc
    26101 tcggtattgc cgggctggtg ggggcgaaac gtaccgatat tgttgagacg ttatttggta
    26161 ttcgcgagaa atcggctggc accattacgt tgcacggcaa acagatcaat aaccataatg
    26221 ccaacgaagc cataaaccac ggatttgcac tggtaactga ggagcgccgc tcaacgggaa
    26281 tttatgccta tctggatatt ggttttaact cgttaatttc caatattcgc aactacaaaa
    26341 ataaagttgg tttactggat aactcgcgga tgaaaagcga tacccagtgg gtgattgatt
    26401 cgatgcgggt aaaaacgccg ggtcatcgga cgcaaattgg ttcgctctcc ggtggtaatc
    26461 agcaaaaggt gattattggt cgctggctac taacgcaacc agaaatatta atgctcgatg
    26521 aaccgacgcg cggtattgat gtcggggcga agtttgaaat ttatcagtta attgccgaac
    26581 tggcgaagaa aggcaagggg attattatta tctcctctga aatgcctgag ttgttaggga
    26641 taacagaccg tattctggtc atgagcaatg gtctcgtttc cggaattgtc gatacaaaaa
    26701 caacaacgca aaacgaaatt ctgcgtcttg cgtctttgca cctttaagat taggggcttc
    26761 ccatgagtgc gttaaataag aaaagttttc ttacttacct gaaagagggc ggtatttacg
    26821 tcgttctttt agttttgctg gcgattatta ttttccagga cccaacattt ttaagtctgt
    26881 tgaacttaag taatattctc acccagtcat cggtgcgtat tattatcgcg ctcggtgtgg
    26941 cagggttaat tgtcacccag gggaccgatc tttctgctgg tcgtcaggta gggctggcgg
    27001 cagtggtggc tgcgacatta ttgcagtcca tggataacgc caacaaagtg ttcccggaaa
    27061 tggcgacgat gccgattgcg ctggttattc tgattgtctg tgccattggt gcggtgatcg
    27121 gtttgatcaa cggtctgatt atcgcttatc tcaacgtgac gccgttcatt accacgctcg
    27181 gcacgatgat catcgtctat ggcatcaact cgctctatta cgactttgtc ggggcgtcgc
    27241 caatttctgg ttttgacagt ggcttctcta cctttgctca gggctttgtc gcgctgggga
    27301 gtttccgtct ctcttacatc accttctacg cgttgattgc ggtggcgttc gtctgggtgt
    27361 tgtggaacaa aacccgcttc ggtaagaaca tttttgccat tggcggtaac ccggaagcgg
    27421 caaaaatatc tggtgtcaac gtcggcctga acctgctgat gatctacgcg ttgtctggcg
    27481 tgttctatgc ctttggcggg atgttagaag ccggacgtat cggctctgcc accaacaacc
    27541 tcggctttat gtatgagctg gatgctatcg cggcgtgcgt ggtaggcggt gtatcgttca
    27601 gcggcggtgt ggggacggtg attggcgtgg tgaccggggt aattattttt accgtcatca
    27661 actatggcct gacgtatatc ggcgtaaacc catactggca gtacatcatc aaaggggcga
    27721 ttattatctt cgccgtagcg ctggattcac tgaaatacgc gcgtaagaag tgatgtgatt
    27781 gcggatattg ccggatgcgt ctctggtttt acaacgttac cgggtgttct ttctcgcctt
    27841 tcttaaactt cacttccccg agctcaatac aacccaccgg gcagacgtga ccacaaagca
    27901 gacaacccac acatttctcg gtattacaat gcggtgtgcg ggttttctcg ctccattcca
    27961 tcgcctggtg accgccgtcg taacaggaaa tataacagcg tccacagcca acacatttgt
    28021 caagattgat acggggatag acaatataac tgcggtctaa atcttccgcc gggacaatat
    28081 tgttattcgc cagacctacc atctcctgca acgaatcaaa tccctgatcg gcgagataat
    28141 gcgataaccc gctcgccata tcttctacga tgcgatagcc gtactgcata atgccggtgg
    28201 tgacctgtaa cgttgctgcg ccgagcaata aaaactccgc cgcatcttcc caggtttcaa
    28261 tgccgccgat accgctgatt gggaaatcgc gcagttctgg atgggtgcgc atttgctgaa
    28321 tgaagcgcag ggcgatcggt tttaccgctt taccggaata tccggaaata ctcgatttac
    28381 cgttaacgat cggcataccg attttctgat taagatcgat attggtgatg gatttaacgg
    28441 tgttaattgc cgcaatgcca tctgcgccgc cgcgcttcgc cgccagcgcc acttcgcaca
    28501 tatcaccgat attcggcgtc atcttcgcca gcattggcag cgtggaaccc cgtttcactg
    28561 cccgacaata tttttctacc agctccgggc tttgcccgac atcgctaccc atcgcatgag
    28621 aagtcatttg cggacaggag aagttacact cgatcatatc cgcgccagct gctcccattg
    28681 ctgctcattt tcccccatga tcgaagcgat caatactttg tccgggtaat cttccttcag
    28741 ccgacgcagg gcggtcagat tctcttccaa cggatgttca gcaatctgct ccatattttt
    28801 gaagccgata aaaccggtat cttctttcac cagatgatca aaacgcggcg agacttcgtt
    28861 ggcgataaaa aagccgatcg ttttaaacac cacaccgccc caacctgtgt cgtaggcttt
    28921 ggcacacatc tcatagcagt tgcctaccgg cgaagaagag aggcagaacg ggttgggaaa
    28981 gttcacgccg caaaaagtaa tcgaaagatc tttcgttaac atgagcaagc tccctctaaa
    29041 tagtgatgaa tcgcctcggc ggcttctttc ccggttttca cggcatagac cacggttttg
    29101 tcaccctcaa caatatcgcc agcagcaaag acttgcggat cgcggatctg ataattttgt
    29161 gttttgatgg tgttacgctg ctgctctaac tccgcaaagg catccagtct ggcatgctga
    29221 ccgacggcga gaataatttt atctgccgcc atcgtcagtt cgcctgataa ccgtacatgc
    29281 ttaaacgtga ctttattgcc ttccacggct actggcgtga atccatcgat gatcgaaacg
    29341 cccagctccc gggcgctggc aaactctttt tcgcttgccg gaaactcatc taactcttca
    29401 cgtgctacgc aagttaccgc ctgacagccg agaactttca gcgtgctggc tacgtccatc
    29461 gcgacatcac cgccgccgat aattaatgcg ctttgtggaa tgctgatatc gccttgcgcc
    29521 tgtcgtacac gttgcaggaa gtcgacggca atctcaacgt cgctatgctc aaacagcggt
    29581 agcccggaac cgcttgataa ccccacggtg accagtaccg cgcggttttc cgctttcagt
    29641 tgctccaggg tgagtgtttt accgatttcg ttattgcact taatggtcac gcccattttt
    29701 tcaatacggg cgatctctgc atccagcact gattgcggta accggaattg cggaataccg
    29761 ttacgcaacc agccaccggg gtgcgcttct ttctcataaa tcgtgacgtc ataaccctgg
    29821 tttgtcagtg tcacactggc ctgcaatccg gcaggacctg cgccaataat cgcgactttg
    29881 ccgagcgttt tagtaccggg ctgataaatt tccatcccgg tttgttgttc aaaatcagta
    29941 acaaaacgct gtaagcggcc gatatcaatg ggagcatcaa caccggcacg ggtacaaccg
    30001 ctttggcata atttctccgt cgggcacact ctggcacaaa cggcaccgag ggcattattt
    30061 tcgcgaattg tctcggcagc gcctttaaaa ttacgaaagt agattgagcg aataaatttc
    30121 cccggatcgg tctgtgccgg gcaagcctga ctacagggag cgtcgtgaca taataaacag
    30181 cgagaggctt ctttaatcgc cagtaacggc gtaaaagccg gagtgagttc atccagataa
    30241 ttttgttgcg gcatgtgatc ttccttaatg ctatgagtaa ttatggcgaa cgctaaataa
    30301 atattgcgtg ctgatattaa ggctatgcgc gtggggaaat ttaagctgtg aaaggattca
    30361 caaatagctg cggttcagaa tgttatatcc gcagcttatt gagtgaagta ataattattc
    30421 tcaataatta acagaaaaga gaaaatatta acgatagaaa ataagataaa caatcatgcc
    30481 gatagataaa attccgaagc caatatatgc caccatataa gcgaagaaga gtccgggatt
    30541 ttttgctcgc cagacataca ccggttccgg tgcattttta acccgtttat caatcgcgcc
    30601 tgcgcgccag cctttccatg cttcgcggaa acaaaatatc gggataagga aaaaaacggc
    30661 aacgacaaaa aactgctgta catccataac tactttcctt gtttcttttg taattcaagt
    30721 aactgttcgg gtgtaacggc gggatacccc tgcgcatctt ccggcacctg gtgcatagcc
    30781 ggaataggga ccagcggccc taaaaaacgc ggttcacgtt taaaaatata aaggtcagcc
    30841 agcgcaccga aacgggcggc aaattcgcga atacgtactg acagcatatc tttcggtgac
    30901 ggtacggcat aacactgagc ctgaatcccc atatgcagcg caataaataa tgctcgctca
    30961 cagtggaaac gttgggtgat aataatgaaa tcattagtat cgaaaacttt gcgtgtacgc
    31021 acgatggaat ccagcgtacg aaagcctgcg taatcgagaa caatatctga tgggtcgaca
    31081 ccagcagcga ttaaatcttt gcgcatggtc atcggctcgt tataactttg caatgcgtta
    31141 tcgccgctca gtaatagata atttacctta ccgctgttat aggcattaat cgctccttga
    31201 atgcggtagc gataatactg attaataacg ccggtacgat aatattttgc tgtgccgagc
    31261 accacgccga cctggcggta ggggagatcc tgcaattcgt cgtagatata aggcgcggtt
    31321 ttccagctca tccagcgatc gaggccgagc acagtcaaca gcagcaagcc gatcaggact
    31381 aacaggctga ggaacacgcg ctttaacatg caggagactc gagctaagga aaatttccat
    31441 caggctactt tacccgctgg ttttgcgcaa gaaacggcaa actgtttgcc gggaatttcg
    31501 acaccaggag agggctggca agccggacaa ggcgttcacg ccgcatccgg caccaggctt
    31561 aagcgagaag cacgcggtcg atactgtgac agccgagagc tttcagcgtt gcggaggtgg
    31621 catcccactg aatcaacggc gcatcggctt tttctgccag aaccgcgcgc tggatatccg
    31681 ggtaatcata acccttgaga ttcaacagaa ttaacgcccc ctgcaacggc ggcagagtcg
    31741 ggttgaatgc ggcgttttca gcgtagctgc cgctgaaaat acgaccgtct ttacattcca
    31801 gcgcgacacc gcttggtgac ttactgtaag gcatgtgcga acggtttgcc gctgcaatcg
    31861 ccgcctgaga aagcgcatca ccagtcagcg catagccgtg atcctgctcg tccatcagca
    31921 gcgttttaat ctccagatct ttcggcccaa aggcatccgg cagatagtca cgcagcgcgt
    31981 gtgcctcgcg gcccggcaaa tgaatacgca gatccagacc gctgttcagt tcattcataa
    32041 actgacggca gtgaccacaa ggcgtatagt taacggtgat ggctgcaagc gctttttcac
    32101 cactcaacca agcgtggctg atcgcgcttt gttcggcatg aacggtttgc tgcattgttg
    32161 caccaataaa ttccatattg gcaccaaaat accaggttcc gctcacaccg cgcgcaattg
    32221 cgccaacatt aaaattcgac aatggcgtac gcgcacaggc cgccgccagc ggaagtagtg
    32281 cgaatgccag cgcgtcttcg tccagtcccg ttgcgctctt cagcgatgag acttgctccc
    32341 cggtcaacaa agcggggaag tacttgtctg ccagaatagg ttccagtgca gattgcaagt
    32401 tatccgcaag ttgggcaaaa gcggtttgaa aacgtggatg catggcgttg cctcataatc
    32461 atgtaatggg ataacagttt acgaacccgt tatggcttta tatgtgatct gaatctcatt
    32521 aattatgcaa cacgtgaaac ttacatcaaa aatgcgcgac gtatcgcaaa ttttagccca
    32581 ttactgccag aataatcggg aaaaggaacg gtgcgatcag cgaagtaatt atcccgcata
    32641 acaccagcgc tagcgaacta aatgcacctt cctgataatc cagctcggcg cagcgcgccg
    32701 taccgagggc gtgcgaggca gtccccattg ccagaccgcg cgcagctttg gtacgaatac
    32761 gcatcgcatt aagcaatgta tggccaaata ccgcgccgag gatgccgacg aaaatcacgc
    32821 aaactgcgct gattgccgga ataccgccaa tactgccgcc aaccgccatt gcaataggcg
    32881 tggtgactga tttcggcagg attgacgcgg cgatttccgg tgaagcgccc atcaacaatg
    32941 ccacggaagt ccccgtcacc atcgcaacca cgctgccgat aaaacagatg gtgatgatcg
    33001 atttccagcg cgcgcggatc tggtgtagct gctcatataa aggataggcc agcgcgacga
    33061 ccgccggttg cagcagatcg tttagcactt cgctaccttt aaagtaactg tcgtaagaga
    33121 tgccggtcag cattaaaaaa ggaatgatca ccaccattgc taccagcaac gggttaagca
    33181 ggggaaactt ataccgtgcc gccagtttgc gggcggcaaa aaagacaatc aacgtcagcg
    33241 gtaatgacca ccagatattc gccatcattc ttctgatcct ttctgaccta cgactttacg
    33301 ttcaccgtgt accagttgtg aactccagct caccaccaga aaaaccacca gcgtactgac
    33361 agcacaggaa accactaccg ggccaaactg cgcgcggagc aaatcaaaat attgcatgac
    33421 gcctacgcca atcggcacaa acaatagcgc catatagcga atcagtacgt agcaccccgg
    33481 attgacccat ttcgccggaa gaatttgcaa ggccagcagg acaaacagga tcagcatccc
    33541 gatgatgctg cccggaatgg ttaccggtag cagggaagca atgaaaatgc ctgcatacag
    33601 gcaggcataa atcaggacga aagcgcgtaa atattgccag ataatgttca gtgtcttact
    33661 cataatcaac accccagatg agatggattc atcatacaat taattcgtaa aacgtgctac
    33721 cgatcacatt aagaaatcag cgcttatttt ttcgctaccc ggtattacgc aatgagcagc
    33781 gaactcaggc atacatcaag catagagagg gattgcagtt tgcagcaatc cctctttgca
    33841 gacctttaat ccgctgacca taggcgctaa catagcgcct catttttttg cgggtgatga
    33901 tatgcgtgtg ttactggcac cgatggaggg agtgcttgac tctctggtgc gtgaattgct
    33961 gaccgaagtt aacgactacg atctgtgcat caccgagttt gtccgcgtgg tggatcaact
    34021 gctgccggta aaagtctttc atcgcatttg ccctgagcta caaaacgcca gccggacacc
    34081 atctggtacg ctggtgcgcg tgcagttgtt aggtcagttc ccacaatggc tggcagagaa
    34141 cgccgcccgt gcggtggagt taggtttctg gggcgtggat ctcaattgcg gctgcccgtc
    34201 gaaaacggtt aacggtagcg gcggcggggc gacgttactc aaagatcctg aactcatcta
    34261 ccagggggca aaagcgatgc gtgaagctgt accggcgcat ttgcctgtca gcgtgaaagt
    34321 gcgtctgggc tgggacagcg gtgagaagaa atttgaaatc gccgatgcgg ttcaacaggc
    34381 tggcgctacg gagctggtgg tgcatgggcg gacgaaagag cagggttacc gcgcggagca
    34441 tattgactgg caggcgattg gcgagattcg ccagcggctg aatattccgg tgattgccaa
    34501 cggtgaaatc tgggactggc agagcgcgca acaatgcatg gcgatcagcg gctgcgacgc
    34561 agtgatgatt ggtcgcgggg cgctcaatat tcccaacctg agccgggtgg taaaatataa
    34621 cgaaccgcga atgccgtggc cggaggtggt tgctttgctg caaaaatata cccgtctgga
    34681 aaagcagggc gataccgggt tatatcacgt tgcgcggatt aaacagtggt tgagttattt
    34741 gcgtaaagaa tacgatgaag caacggaatt atttcagcat gttcgggtgt tgaataactc
    34801 ccctgatatt gcaagggcta ttcaggcaat tgatatcgag aaactctaaa aaccatagtg
    34861 atgtttttga caatgtgtta gctctttatc aataggccgc acggttgttt ggtggcgtca
    34921 aaatcgccga ggcgttccct gaaggccggg gcagcccaca tggatgtggg ctgagggcgc
    34981 gttttacagg gatgttacct cgcgcccgac ccggtagccg taagggataa gccgagggca
    35041 ccgcgcagcg gcgattttgt tcgccagagc ccgggggggc agggggcggc ggcgattggt
    35101 cgccccctgc gcgctccttg cgccagtgat tatatgaagc aatgcacatg acaggagcgc
    35161 aacaggatga gagactgaaa tattctcgtt attttcggat ctgatgacat taattaattt
    35221 taaaatatca tcttaaatac gccagtcacc accagtagcc caatcaggaa aataatcaat
    35281 acagcccaga gtataatttt cattggcgtt cctttatttg tgagtgctga ataagtatag
    35341 gaaaaaacga agccctttgc cgttatccat aaacgattaa gataaatcgg aagatatcat
    35401 ttacttagtc tatatttttc tgggtatgtc gttttattgc tactgatgta ttgccacgcg
    35461 aaactaaaaa atatatcgca acacacggta atctccgcgc accgtttctt acctgattat
    35521 tcccccatga atcgtgattc cttttatcca gccatcgcct gttttccgct gttactgatg
    35581 ctggccgggt gtgcgcctat gcatgaaacc cgccaggcgt taagccagca aacgcccgct
    35641 gcacaagttg acaccgcatt acccacggcg ctgaaaaatg gttggccaga cagccaatgg
    35701 tggctggagt atcacgataa tcagctcact tcattaatta acaatgcact acagaacgca
    35761 ccggatatgc aggtggctga acagcgcatt tagctagctg aagcccaggc gaaggcagtt
    35821 gccactcagg atggtccgca gatcgacttt tcggcggata tggagcggca aaaaatgtcg
    35881 gcagaagggc taatggggcc gtttgctctg aacgatccgg ccgcaggtac gaccggtccg
    35941 tggtacacca acggaacttt tggcttaacg gcgggctggc atctcgatat ctggggaaag
    36001 aatcgggcgg aggttactgc ccgcctgggt acggttaaag cacgggcggc ggaacgcgag
    36061 caaacccgcc aattgctggc tggcagcgta gcccgcctgt actgggagtg gcaaacccag
    36121 gcggcgttaa acacggtctt gcagcaaata gaaaaagagc agaacaccat tatcgcgacc
    36181 gatcgccagc tatatcagaa cgggattact tcttcagttg acggtgtgga aaccgatatt
    36241 aatgccagca aaacccggca gcagctcaac gatgtcgcgg ggaaaatgaa aattattgag
    36301 gcacggttaa atgcactcac aaatcaccag actaagtcat tgaagcttaa accggtcgcg
    36361 ttgccgaaag tggcaagcca gcttcctgat gaactggggt actccttact ggcccggcgg
    36421 gcagatttgc aggcggcgca ctggtacgtt gagtcatcgt taagcactat tgatgcggca
    36481 aaagcggcat tttatcctga catcaacctg atggccttcc tgcaacagga tgcgttgcac
    36541 ttaagcgatc tgttccgtca ttccgcgcag caaatgggcg ttacggcagg cctgacgcta
    36601 cctattttcg atagtggtcg tcttaacgcc aatctcgata tcgcaaaagc cgaaagcaac
    36661 ttgtctatcg ccagctacaa caaagcggtg gttgaagcag tgaatgacgt ggcgcgggca
    36721 gccagtcagg ttcagacact ggcggagaaa aaccagcatc aggcgcaaat tgagcgcgat
    36781 gccttgcgtg tggtaggtct tgcgcaggcc cgctttaacg cgggcatcat tgctggttcc
    36841 cgcgtcagcg aagccagaat cccggcgctg cgtgagcggg ccaatggcct gttattgcaa
    36901 gggcagtggc tggatgcctc cattcgactc accggtgcgc tgggcggggg ttacaaacgc
    36961 tgatgatatg cgccttctat acttaacgtt tattcagcgt taagtggaga actcgatggc
    37021 ataggtcgcg ataattaccg cttccgattc ggggatcggc aaagagtgcg cgttattact
    37081 ggcgcagcag gggtttgata ttggcattac ctggcactca gatgaagaag gggcaaaaga
    37141 taccgcgcgt gaggtggtta cccacggcgt acgtgcggag atcgtgcaac tggatctcgg
    37201 caatctgcca gaaggtgcgc aggcgctgga gacactcatc cagcgatttg ggcgtattga
    37261 tgtgctggtg aataatgcgg gtgcaatgac caaagcgccg tttcttgata tggcttttga
    37321 tgagtggcgc aagattttta ccgttgatgt tgatggtgca tttttatgct cgcaaattgc
    37381 ggctcgtcag atggtgaaat aagggcaggg cggtcgcatc atcaacatta cgtcggtaca
    37441 tgaacatacg ccgctgccgg atgccagcgc ctacacagcc gctaaacatg cgctcggcgg
    37501 gttaaccaaa acgatggcgc tggagttggt caggcataag attttggtga acgcagtcgc
    37561 gcctggggcg atagccacgc caatgaacag catggatgat agcgacgtga agcccgacgc
    37621 ggagccttcg attcctctac ggcgttttgg cgcaacgcat gagattgcca gcctggtggt
    37681 gtggctttgt tcggagggcg caaattacac gacagggcag tcgttgatag tggatggcgg
    37741 ctttatgttg gcgaatccgc agttcaaccc agaatagcgc agtgaggtgc gtggatattt
    37801 caatgggtta ggccagataa gacaccagcg tcgcatcagg catggaacac ccggtgccgg
    37861 atgtggagtg aatgcttatc tgtggcaact gctttacgcc tgattatccg gctttttctt
    37921 cccgcgccgt ttcagccacc agcgcacgcc caccaccaga accacgacca gaatcaacca
    37981 gacccagtgc ttcaaatgct ggtcgagatt gtgcaaccac ggcgcaatca cctgaccacc
    38041 agcgtaacca atagtggtaa aaatcaacgc ccaggcaaat gcgccgagaa tattcagcgg
    38101 cagaaagatt ttcggcggca gctggctggc accaatcagc gtcgggccaa tcacccgaaa
    38161 gccatacata aagcgcgtac caatgacaaa cagatacgga tggcgttgga taagtttctg
    38221 tgcccgctca attttatcct gatgtttcga gaaacggcgt aacagcttgc cgccaaaccg
    38281 ccgcccgcac agatagagca cctggtcgcc aatcatgcca ccaagcgcca cagaaagtac
    38341 caccagtggg aactttaata gcccctgatg tgccgcaacg cctcccagca aagtcacggt
    38401 ttcaccttcc gccaggctac cgataaccag cgcggcataa ccatattgtg agatgagtgt
    38461 attgagatcc ataggccagt ttgtctccct ttggttgcct gtaagcatac accctgtgaa
    38521 tcattcacgc tgaaaaagga catacatcag acggtgcaaa ttacatggat gaacaaaaaa
    38581 taatcacaag ctggcttata cttgaactgt caggttcatg aactcacaac aagggggcgg
    38641 tatgagccat gtctggggac tgttttccca tcccgatcgt gaaatgcagg tgattaatcg
    38701 cgaaaacgaa acgatttctc accattacac ccaccacgtt ttgctgatgg cggcgattcc
    38761 ggtgatttgc gccttcattg gcactacaca gattggctgg aattttggcg atggcactat
    38821 cctgaagtta tcctggttta ccggactggc gctggctgtc ttgttttacg gcgttatgct
    38881 ggctggggtt gcggtgatgg ggcgggtcat ctggtggatg gcgcgcaatt acccgcagcg
    38941 tccgtctctt gcgcactgca tggtcttcgc gggctacgtt gcaactccgc tgtttttaag
    39001 cggtctggtg gcgctttatc cactggtctg gttgtgtgcg ctggtcggca cggtggcgct
    39061 gttttacacc ggctacctgc tttaccttgg tattccgagc ttcctgaata tcaacaaaga
    39121 ggaaggcctt agcttctcca gttcgacgct ggcgattggc gtgctggtac tggaagtgct
    39181 gctcacccta accgttatac tatggggcta cggttatcgt ttgttctaga aagttctttg
    39241 acgttgcatt gctggcgtaa atgtcagcaa tgcagcattt actcacgatt ctcctttgac
    39301 gatcgcactt tttgctcgtt atgatggcgt cgctagcctc agtaaatcct tactccggcg
    39361 gtgcgcaacc cgtgcgcgtg aaccactatc tgaatgctca tcatgccgaa atttcgagtt
    39421 tctttattta gcttggccct gatgctggct gtgccttttg caccgcaggc cgttgcgaaa
    39481 acggcagccg ctaccaccgc ttcacaaccg gaaattgcct ccggtagcgc gatgattgtt
    39541 gatctgaata ccaacaaagt gatctattcg aaccacccgg atctggtgcg tccgattgcg
    39601 tctatcagca aattaatgac cgcgatggtt gtgctggatg cacgactgcc gctggatgaa
    39661 aaactaaaag tggatatcag ccagacgccg gagatgaaag gggtctattc gcgcgtacga
    39721 ctgaatagcg aaatcagccg taaagatatg ctgttgctgg cgctgatgtc ttcagaaaac
    39781 cgcgcggcgg caagccttgc gcaccattat cccggtggtt acaaagcctt tattaaggca
    39841 atgaatgcga aagcgaaatc gctcggaatg aacaacacgc gctttgttga acctaccgga
    39901 ttgtcggtgc ataacgtttc aactgcccgt gacttaacca aactgctcat tgccagcaaa
    39961 caatatccgt tgatcgggca gttaagtact acccgggaag atatggcaac cttctctaac
    40021 ccgacgtata cgctgccgtt ccgcaatact aatcatctgg tgtatcgcga taactggaat
    40081 attcagttaa ccaaaaccgg ctttaccaat gcggcgggcc attgtctggt gatgcgtacg
    40141 gttatcaata ataaaccggt ggcgctggta gtgatggacg cgtttggcaa atatacccat
    40201 tttgccgatg ccagccgcct gcgtacctgg attgaaaccg gtaaagtgat gcctgtgccg
    40261 gcagcagcgt tgagctataa aaaacaaaaa gccgcccaaa tggcggcggc ggggcagacg
    40321 gcacagaacg attaagtgaa ttcggatggc gatactctgc catccgtaat ttttactcca
    40381 cttcctgcca gtttttccgt ttactggttt taccgatccc cggattcatg ctattggtcg
    40441 gatcattctc gcgatagaac ttctgcaacg tctccggtgc tttatacaaa tgaccgacgt
    40501 tatgctcggc agggtactgc gcgccgcgct gctgtagcag ttccagcatc tgttctttta
    40561 acgcatgcac atccacgcct tttttcacta tgtaatcctg atggaagaca tagcacataa
    40621 aatggccgta atagagcttg tgcaccagct ggctgtcgat ctccggcggt aaatgctcat
    40681 accactcggt gtcgttacgc cgcagagcga tatccagtgc cagaatgtct tcgacttcat
    40741 cggaatgcac cgcctgataa cgaattgctg cgcccgcagc ggcgaaacgg tgtaaaaacg
    40801 ctttgctacc ttcctccggc gtacagacaa agaaatcgcc ctcggcctgt ttgaaataat
    40861 ccaccagcca cgatttggct tcaccgacgc catcgcctgc catttttaac agcagatgat
    40921 gctcgtattt atcgcgccag tttttcatgc gcggcggtaa atggctgggg aacaggtgac
    40981 cgaatttttg cattgcacgg tcagtaaaat gcggacggaa gaatttcact ttctccagca
    41041 tcgcatcggt gcgtcccttg agattaaaga agaacggcat cttgtcggtg cctaacttat
    41101 caatcatcag gaaggtgtct ttgccgtatt tttccgcaat atcgtagata tcccggtgca
    41161 tatattcccc ggcaaccggc agattttcga agttagccag aatatgacgg cggatttcgg
    41221 tcagcacttc cggctggttg gtgccgatat aaaacacctg ctgatttttt tccgcttcga
    41281 aggtatcaag acgtaccgca aagacggcca gcttcccggc gcaaccagaa gattcaaata
    41341 accgatccgg atcggcgtta taacgtgcgg gcgtgtcggc ttcaatatca cgaacgcggt
    41401 ggacatagtc ataatcgtgg gcgtgacgac catcgtgacg cacatcgtca tctttgatgc
    41461 gatcgtcatc cagcttgcta aggatctgct ccggcgtttc gcccagatca atccccagat
    41521 ggttcaccag cgtcagtttg ccatcttcat ctatacgcgc gaataacgac atttcggtat
    41581 acgccgggcc acgttgcacc agcgagccgc cggagttgtt acaaataccg ccgatgaccg
    41641 atgcgcctat acacgatgat ccaatcaccg aatgcggttc gcgtcccagc ggtttgaggg
    41701 ctttttccag cgaatagagc gtggtgcccg gataggccag cacctgttcg cccttgccga
    41761 gaacgtgcag cttgtcgaga cgcagggtgc tgatgatcac gatatcgcga tcatagtcgt
    41821 taccgtttgg cgtcgatcct tcggtcaggc ctgtattggc agcctgcatc agaataattt
    41881 tgtcggcggt gacgcaggct ttcagcaccc gccacaattc cagtagtgag ccagggaaaa
    41941 cgacagccag cgcgtcgccc tgaccagaac ggaagccctt gcgatagcgg gccgtttttg
    42001 cgggatcggt gagcaggtgt gaatgaccga ccagacgggc aagttcattc aaaaaggctt
    42061 tattatcagt tgttgtcatg gaagacattt tccactcctt gtggtggcga aaaaatatca
    42121 caaactagaa tagcgccttg tatgacaatt cagcgagtga atattttgaa aattcagaca
    42181 ataaactgag cattcttttt ctctctccat catgcttatg gcaaactacg gtttttgcac
    42241 cattttcgct gccacgtcgg gcaacaaagg aagaaaaatc catatgaaat ggctatgttc
    42301 agtaggaatc gcggtgagtc tggtcctgca gccagcactg gcggatgatt tattcggtaa
    42361 ccatccatta acgcctgaag cgcgggatgc gttcgtcacc gatctgctca agaaaatgac
    42421 ggttgatgag aaaattggtc agctgcgctt aatcagcgta ggcccggata atccgaaaga
    42481 ggcgatccgc gagatgatca aagacggtca ggttggggcg attttcaaca ccgtaacccg
    42541 tcaggatatc cgcgccatgc aggatcaggt gatggaatta agccgcctga aaattcctct
    42601 tttctttgct tacgacgtgc tgcacggtca gcgcacggtg ttcccgatta gcctcggtct
    42661 ggcctcgtct tttaacctcg atgcggtgaa aacggtcggg cgtgtctctg cttatgaagc
    42721 ggcagatgat ggcctgaata tgacctgggc accgatggtc gatgtctcgc gcgatccgcg
    42781 ctggggacgt gcttccgaag gttttggcga agatacgtat ctcacctcaa taatgggtaa
    42841 aaccatggtg gaagcgatgc agggtaaaag cccggcagat cgttactcgg tgatgaccag
    42901 cgtcaaacac tttgccgcat acggcgcggt agaaggcggt aaagagtaca acaccgtcga
    42961 tatgagtccg cagcgcctgt ttaatgatta tatgccgccg tacaaagcgg ggctggacgc
    43021 aggcagcggc gcggtgatgg tggcgctgaa ctcgctgaac ggcacgccag ccacctccga
    43081 ttcctggctg ctgaaagatg ttctgcgcga ccagtggggc tttaaaggca tcaccgtttc
    43141 cgatcacggt gcaatcaaag agctgattaa acatggcacg gcggcagacc cggaagatgc
    43201 ggtgcgcgtg gcgctgaaat ccggaatcaa catgagtatg agcgacgagt actactcgaa
    43261 gtatctgcct gggttgatta aatccggcaa agtgaccatg gcagagctgg acgatgctgc
    43321 ccgccatgta ctgaacgtta aatatgatat ggggttgttt aacgacccat acagccattt
    43381 ggggccgaaa gagtctgacc cggtggatac caatgccgaa agccgcctgc accgtaaaga
    43441 agcgcgtgaa gtggcgcgcg aaagcctggt gttgctgaaa aaccgtctcg aaacgttacc
    43501 gctgaaaaaa tcggccacca ttgcggtggt tgggccactg gcggacagta aacgtgacgt
    43561 gatgggcagc tggtccgcag ccggtgttgc cgatcaatcc gtgaccgtac tgaccgggat
    43621 taaaaattcc gtcggtgaaa acggtaaagt gctgtatgcc aaaggggcga acgttaccag
    43681 tgacaaaggc attatcgatt tcctgaatca gtatgaagaa gcggtcaaag tcgatccgcg
    43741 ttcgccgcaa gagatgattg atgaagcggt gcagacggcg aaacaatctg atgtggtggt
    43801 ggctgtagtc ggtgaagcac aggggatggc gcacgaagcc tccagccgga ccgatatcac
    43861 tattccgcaa ggccaacgtg acttgattgc ggcgctgaaa gccaccggta aaccgctggt
    43921 gctggtgctg atgaacgggc gtccgctggc gctggtgaaa gaagatcagc aggctgatgc
    43981 gattctggaa acctggtttg cggggactga aggcggtaat gcaattgccg atgtattgtt
    44041 tggcgattac aacccgtccg gcaagctgcc aatttccttc ccgcgttctg tcgggcagat
    44101 cccggtgtac tacagccatc tgaacaccgg tcgtccgtat aatgccgaca agccgaacaa
    44161 atatacttcg cgttattttg atgaagctaa cggagcgctt tatccgttcg gctatggtct
    44221 gagctatacc actttcaccg tctctgatgt gaaactttct gcgccgacca tgaagcgtga
    44281 cggcaaagtg acggccagcg tgcaggtgac gaacaccggt aagcgcgagg gggccacggt
    44341 agtgcagatg tacttgcagg atgtgacggc ttccatgagt cgccctgtga aacagctgaa
    44401 aggctttgag aaaatcaccc tgaaaccggg cgaaactcag actgtcagct tcccgatcga
    44461 tattgaggcg ctgaagttct ggaatcaaca gatgaaatat gacgccgagc ctggcaagtt
    44521 caatgtattt atcggcactg attccgcacg cgttaagaaa ggcgagtttg agttgctgta
    44581 atgctttagt aagggcgcga cgtttgcgcc ctttgcctga tgttcatatc ttaacttgct
    44641 gaattttcgc cctttgtagg ccggataagg cgctcgcgcc gcatccggca ggaaacgccg
    44701 gaccgaatat gcctcttaag tatttatctg attctgcaac tgaatccttc cgctcaggct
    44761 aactctgtca ttatcaacta tgcttttctc ttaattcgct gaaaacaaca gtaaaagaat
    44821 gaggaaagca tcatgccact cttaaagctc tgggcaggtt cactggttat gttggcagcc
    44881 gtgagcctgc cgctacaggc ggcttccccc gttaaagtcg gttcaaaaat cgataccgaa
    44941 ggcgcgctgc tcggcaacat catcttgcag gtactcgaaa gccacggcgt accgacggtg
    45001 aataaagtgc aactcggaac gactcctgtg gggcgcgggg cgattacttc cgatgaactg
    45061 gatatctatc cggaatatac cggcaatggc gctttcttct ttaaagatga aaacgatgca
    45121 gcgtggaaaa atgccgggca gggctacgag aaagtcaaaa agctcgatgc ggagcacaac
    45181 aagttaatct ggctgacgcc cgcgcctgca aataacacct ggactatcgc cgtgcgtcag
    45241 gatgtggcag agaaaaacaa actcacttcg cttgccgacc tgagtcgtta tctgcaagag
    45301 ggcggcacct tcaaactggc agcctcggca gagtttatcg aacgcgccga tgcgttaccc
    45361 acgtttgaaa aagcctatgg ctttaagctc ggtcaggatc agttgctgtc actggctggt
    45421 ggcgacacgg cggtgacgat taaagccgct gcccagcaaa cctctggcgt taatgccgca
    45481 atggcttacg gcactgacgg tccggtcgcg gcgctggggc tgcaaacctt aagcgatccg
    45541 caaggcgtgc aacctatcta cgcgcctgca ccagtggtgc gtgaatcggt gttgaaggag
    45601 tatccgcaaa tggcacagtg gctacagcca gtcttcgcca gcctcgatgc aaaaacattg
    45661 cagcaactga atgccagcat tgcagtggaa ggactggatg ccaaaaaagt ggctgccgac
    45721 tacctgaaac aaaaagggtg ggcgaagtaa tttcccgtga cttatctacg tattaatcct
    45781 gttctggcgc tgctgttgtt gctgacggca atcgcagcgg cgctgccgtt tatcagttac
    45841 gcgcctaatc gtttagtctc gggtgaaggg cttcatctct ggcaactgtg gccgcaaacg
    45901 atctggatgc tggtgggcgt tggttgcgcc tggctgacag cctgttttgt ccccgctaaa
    45961 aaaggcagca tttttgcact cattctggcg caattcgtct tcgtattgtt ggtgtgggga
    46021 gctggaaagg cggcgaccca actggcgcaa aatggcagtg cgctggcgcg taccagcctc
    46081 ggcagaggtt tctggctggc tgcggcgctg gcattgctgg cctgtagcga tgccatccgc
    46141 cgaatctcca cgcatccgct gtggcgctgg ttgttgcata tgcagattgc cattattccg
    46201 ctgtggttgc tgtactccgg cacgcttaac gatctctcac taatgaaaga atacgccaac
    46261 cgtcaggatg tgtttgacga cgcgctggca caacatctga cgttgctgtt tggtgcggtg
    46321 ctgcctgcgt tagtgattgg tgtgccgttg ggcatctggt gctacttttc cactgcgcgg
    46381 cagggggcaa ttttttctct gctcaatgtc attcagaccg tgccttcggt ggcgctcttt
    46441 ggcctgttga ttgcgccgct tgccgcgctg gttacggcct ttccgtggct ggggaagctc
    46501 ggcatagcag gaaccggaat gacacccgca ctgattgcgc tggtgctcta tgccttgctg
    46561 ccgctggtgc gaggcgtggt agtcggtttg aaccagatcc cgcgcgatgt gctggagagc
    46621 gccagagcga tggggatgag cggggcgcag cgtttcctgc atgttcagtt accgctggcg
    46681 ttaccggtat ttctgcgcag cctgcgggtg gtgatggtgc aaactgtagg tatggcggtg
    46741 attgcggcgt taatcggcgc aggcggtttt ggtgcgctgg ttttccaggg gctgctaagc
    46801 agcgccattg atttagtgtt gctgggggtg atcccggtaa ttgttctggc ggtgcttacc
    46861 gacgcgctgt tcgatttgct tatcgcactg ctgaaggtga aacgtaatga ttgaatttag
    46921 ccatgtcagc aaactgttcg gcgcacaaaa agccgttaac gatctcaatc tcaattttca
    46981 ggaagggagt ttttcggtgc tgattggcac atctggctcc ggcaaatcca ccaccctgaa
    47041 aatgattaac cgcctggtgg agcatgacag cggagtgatc cgctttgccg gagaagaaat
    47101 tcgctcgctg ccagtactgg agttgcgccg ccggatgggc tatgccattc aatctattgg
    47161 cctgttcccc cactggagcg tggcgcaaaa catcgctacc gtgccgcaat tacaaaaatg
    47221 gtcgcgggcg cggattgacg atcgtatcga cgaattaatg gcgctactgg ggctggagtc
    47281 aaatttgcgt gagcgttatc cgcatcagct ttccggtggt cagcagcaac gtgtgggagt
    47341 ggcgcgtgca ctggctgccg atccgcaagt cttactgatg gatgaacctt ttggcgcact
    47401 ggacccggta acgcgcggcg cgttgcaaca agagatgacg cgcattcacc gtttgctggg
    47461 gcgcactatt gtgctggtca ctcatgatat tgatgaggcg ctacggctgg cagaacatct
    47521 ggtattgatg gatcacggtg aagtggtgca gcaggggaat ccgctgacga tgctgactcg
    47581 tccggcgaat gattttgtcc gccagttttt tggacgtagt gaactggggg tgcacctgct
    47641 ttcgttacgt agcgtggcgg attacgtgcg tcgcgaagaa cgggcagaag gtgaggcact
    47701 ggcagaagag atgacgctac gcgatgcgct ctccctgttt gtcgcgcggg gatgcgaggt
    47761 gctgccggtg gtgaacacgc agggccagcc ttgcggcacg ctgcattttc aggatctgct
    47821 ggtggaggcg taagcgtatg aagatgttgc gcgatccgct gttctggctc attgctcttt
    47881 ttgtggcgct gattttctgg ctgccttaca gccagccgct gtttgctgcc ttgttcccac
    47941 aactgccacg acccgtttat cagcaagaaa gttttgcagc tctggcactg gctcatttct
    48001 ggctggtggg aatttcgagt ttgtttgcgg tgatcattgg cacaggtgcc ggaattgctg
    48061 ttactcgccc gtggggcgcg gaatttcgcc cactggtgga aactattgcc gccgttggac
    48121 agacttttcc gcctgtcgca gtgctggcga ttgccgttcc ggtgatcggc tttggtctgc
    48181 aaccagcgat tatcgccttg atcctttacg gtgtgctgcc catcctgcag gtgacacttg
    48241 ccgggctggg agcgattgat cccagcgtga cagaagttgc gaaaggtatg ggaatgagtc
    48301 gaggtcagcg actgcgtaag gtcgagctac cgctggcggc tccggtgatt ctggcgggcg
    48361 tgcgaacttc ggtgattatc aacattggta cggcgacgat cgcctcaacg gtaggggcca
    48421 gtacgctggg tacgcccatc atcatcgggc ttagcggatt taataccgcg tatgtgatcc
    48481 agggggcgtt actggtggca ctggcggcga tcatcgcaga ccgcctgttt gaaaggctgg
    48541 tgcaggcgct tagccagcac gcaaaataaa ggtataacct gcgagcatga cgccaccaat
    48601 tccgcctaac gccataaaca ggaacagggc gatgacccca attttagcta tgcgcataat
    48661 gcactcctta tgttaacgaa aggattgtac agtaaagcgc atttgttaac gaatcattaa
    48721 ataccgagtg ggaaaatatc atggccttgt tcttgccaac tggtgagttg ctgctgttgg
    48781 gcggaggttc gattttcacc gcaccacacc agcaatgtac ggccttcgaa tagttcaggg
    48841 cgtagttgat tgagcgagtg ggcgagcgag gacatcaatg cgccatcctt gttgactggc
    48901 aatccagccc tccagccaca gacgggtggt atcctgaata ttccagccaa ccaccagcgc
    48961 atctttaccc tgttttttac gtgccgaagc c
//
LOCUS       DAJTRH010000021        48322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTRH010000021 DAJTRH010000000
VERSION     DAJTRH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            187..480
                     /locus_tag="OO339_001306"
     CDS             187..480
                     /locus_tag="OO339_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5925999.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO339_001307"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO339_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5926000.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="OO339_001308"
     CDS             complement(1922..2374)
                     /locus_tag="OO339_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5926001.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO339_001309"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO339_001309"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5926002.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="OO339_001310"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="OO339_001310"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5926003.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO339_001311"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO339_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5926004.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO339_001312"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO339_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5926005.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO339_001313"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO339_001313"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5926006.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO339_001314"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO339_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5926007.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO339_001315"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO339_001315"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5926008.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO339_001316"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO339_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5926009.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO339_001317"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO339_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5926010.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="OO339_001318"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="OO339_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5926011.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="OO339_001319"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="OO339_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5926012.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="OO339_001320"
     CDS             complement(15085..15582)
                     /locus_tag="OO339_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5926013.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="OO339_001321"
     CDS             complement(15579..15941)
                     /locus_tag="OO339_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5926014.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="OO339_001322"
     CDS             complement(15931..16278)
                     /locus_tag="OO339_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5926015.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="OO339_001323"
     CDS             16387..16800
                     /locus_tag="OO339_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926016.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="OO339_001324"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="OO339_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="OO339_001325"
     CDS             complement(18324..20039)
                     /locus_tag="OO339_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5926017.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGVEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="OO339_001326"
     CDS             20206..21099
                     /locus_tag="OO339_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5926018.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="OO339_001327"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="OO339_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="OO339_001328"
     CDS             complement(21910..23526)
                     /locus_tag="OO339_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5926019.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="OO339_001329"
     CDS             23821..24495
                     /locus_tag="OO339_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5926020.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="OO339_001330"
     CDS             complement(24534..26195)
                     /locus_tag="OO339_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5926021.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO339_001331"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO339_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5926022.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO339_001332"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO339_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5926023.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="OO339_001333"
     CDS             27649..27981
                     /locus_tag="OO339_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5926024.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29215)
                     /locus_tag="OO339_001334"
     CDS             complement(27983..29215)
                     /locus_tag="OO339_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5926025.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="OO339_001335"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="OO339_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5926026.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="OO339_001336"
     CDS             complement(30377..31714)
                     /locus_tag="OO339_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5926027.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OO339_001337"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OO339_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OO339_001338"
     CDS             complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OO339_001338"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5926028.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OO339_001339"
     CDS             complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OO339_001339"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5926029.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OO339_001340"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OO339_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5926030.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /locus_tag="OO339_001341"
     CDS             35377..36033
                     /locus_tag="OO339_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926031.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OO339_001342"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OO339_001342"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5926032.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37403)
                     /locus_tag="OO339_001343"
     CDS             complement(37005..37403)
                     /locus_tag="OO339_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5926033.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OO339_001344"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OO339_001344"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5926034.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OO339_001345"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OO339_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5926035.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OO339_001346"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OO339_001346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5926036.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OO339_001347"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OO339_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5926037.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="OO339_001348"
     CDS             43964..44044
                     /locus_tag="OO339_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926038.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="OO339_001349"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="OO339_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5926039.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="OO339_001350"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="OO339_001350"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5926040.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="OO339_001351"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="OO339_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5926041.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="OO339_001352"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="OO339_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5926042.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="OO339_001353"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="OO339_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5926043.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11981 a        13066 c        12219 g        11056 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaacg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca gaatagacaa aa
//
LOCUS       DAJTRH010000022        47725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTRH010000022 DAJTRH010000000
VERSION     DAJTRH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO339_001354"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO339_001354"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5926044.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO339_001355"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO339_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5926045.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO339_001356"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO339_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5926046.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO339_001357"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO339_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5926047.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO339_001358"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO339_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5926048.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO339_001359"
     CDS             5377..5835
                     /locus_tag="OO339_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5926049.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO339_001360"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO339_001360"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5926050.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO339_001361"
     tRNA            6887..6971
                     /locus_tag="OO339_001361"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO339_001362"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO339_001362"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO339_001363"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO339_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5926051.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO339_001364"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO339_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5926052.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO339_001365"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO339_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5926053.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO339_001366"
     CDS             complement(10252..13995)
                     /locus_tag="OO339_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5926054.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO339_001367"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO339_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5926055.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO339_001368"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO339_001368"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5926056.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO339_001369"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO339_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5926057.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO339_001370"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO339_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5926058.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO339_001371"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO339_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5926059.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO339_001372"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO339_001372"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5926060.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO339_001373"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO339_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5926061.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO339_001374"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO339_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5926062.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO339_001375"
     CDS             23392..23499
                     /locus_tag="OO339_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5926063.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO339_001376"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO339_001376"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5926064.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO339_001377"
     CDS             complement(24538..25347)
                     /locus_tag="OO339_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5926065.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPPAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO339_001378"
     CDS             complement(25351..25743)
                     /locus_tag="OO339_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5926066.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO339_001379"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO339_001379"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5926067.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO339_001380"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO339_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5926068.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO339_001381"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO339_001381"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5926069.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO339_001382"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO339_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5926070.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO339_001383"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO339_001383"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5926071.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO339_001384"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO339_001384"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5926072.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO339_001385"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO339_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5926073.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO339_001386"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO339_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5926074.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO339_001387"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO339_001387"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5926075.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO339_001388"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO339_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5926076.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO339_001389"
     CDS             36790..39657
                     /locus_tag="OO339_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5926077.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO339_001390"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO339_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5926078.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO339_001391"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO339_001391"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5926079.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO339_001392"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO339_001392"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5926080.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO339_001393"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO339_001393"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5926081.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO339_001394"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO339_001394"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5926082.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO339_001395"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO339_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11984 a        12298 c        11685 g        11758 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg gcgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggagg
//
LOCUS       DAJTRH010000023        47429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTRH010000023 DAJTRH010000000
VERSION     DAJTRH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            74..556
                     /gene="sseA"
                     /locus_tag="OO339_001396"
                     /pseudo
     CDS             74..556
                     /gene="sseA"
                     /locus_tag="OO339_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1375..2151)
                     /gene="sseB"
                     /locus_tag="OO339_001397"
     CDS             complement(1375..2151)
                     /gene="sseB"
                     /locus_tag="OO339_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5926083.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2293..3576)
                     /gene="pepB"
                     /locus_tag="OO339_001398"
     CDS             complement(2293..3576)
                     /gene="pepB"
                     /locus_tag="OO339_001398"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5926084.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3635..3835)
                     /gene="iscX"
                     /locus_tag="OO339_001399"
     CDS             complement(3635..3835)
                     /gene="iscX"
                     /locus_tag="OO339_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5926085.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3847..4182)
                     /gene="fdx"
                     /locus_tag="OO339_001400"
     CDS             complement(3847..4182)
                     /gene="fdx"
                     /locus_tag="OO339_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5926086.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4184..6034)
                     /gene="hscA"
                     /locus_tag="OO339_001401"
     CDS             complement(4184..6034)
                     /gene="hscA"
                     /locus_tag="OO339_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5926087.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6051..6566)
                     /gene="hscB"
                     /locus_tag="OO339_001402"
     CDS             complement(6051..6566)
                     /gene="hscB"
                     /locus_tag="OO339_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5926088.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6662..6985)
                     /gene="iscA"
                     /locus_tag="OO339_001403"
     CDS             complement(6662..6985)
                     /gene="iscA"
                     /locus_tag="OO339_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5926089.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7002..7388)
                     /gene="iscU"
                     /locus_tag="OO339_001404"
     CDS             complement(7002..7388)
                     /gene="iscU"
                     /locus_tag="OO339_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5926090.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7416..8630)
                     /gene="iscS"
                     /locus_tag="OO339_001405"
     CDS             complement(7416..8630)
                     /gene="iscS"
                     /locus_tag="OO339_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5926091.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8742..9230)
                     /gene="iscR"
                     /locus_tag="OO339_001406"
     CDS             complement(8742..9230)
                     /gene="iscR"
                     /locus_tag="OO339_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5926092.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9500..10240)
                     /gene="trmJ"
                     /locus_tag="OO339_001407"
     CDS             complement(9500..10240)
                     /gene="trmJ"
                     /locus_tag="OO339_001407"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5926093.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10359..11162
                     /gene="suhB"
                     /locus_tag="OO339_001408"
     CDS             10359..11162
                     /gene="suhB"
                     /locus_tag="OO339_001408"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5926094.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11307..12161
                     /locus_tag="OO339_001409"
     CDS             11307..12161
                     /locus_tag="OO339_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5926095.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12352..13632
                     /gene="csiE"
                     /locus_tag="OO339_001410"
     CDS             12352..13632
                     /gene="csiE"
                     /locus_tag="OO339_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5926096.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13624..14763)
                     /locus_tag="OO339_001411"
     CDS             complement(13624..14763)
                     /locus_tag="OO339_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5926097.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14923..15813)
                     /gene="hcaR"
                     /locus_tag="OO339_001412"
     CDS             complement(14923..15813)
                     /gene="hcaR"
                     /locus_tag="OO339_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5926098.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15949..17310
                     /locus_tag="OO339_001413"
     CDS             15949..17310
                     /locus_tag="OO339_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5926099.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17307..17825
                     /locus_tag="OO339_001414"
     CDS             17307..17825
                     /locus_tag="OO339_001414"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5926100.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17825..18145
                     /locus_tag="OO339_001415"
     CDS             17825..18145
                     /locus_tag="OO339_001415"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5926101.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18142..18954
                     /gene="hcaB"
                     /locus_tag="OO339_001416"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18142..18954
                     /gene="hcaB"
                     /locus_tag="OO339_001416"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5926102.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18964..20186
                     /locus_tag="OO339_001417"
                     /pseudo
     CDS             18964..20186
                     /locus_tag="OO339_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20283..20705
                     /locus_tag="OO339_001418"
     CDS             20283..20705
                     /locus_tag="OO339_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5926103.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20753..21625)
                     /locus_tag="OO339_001419"
     CDS             complement(20753..21625)
                     /locus_tag="OO339_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5926104.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21637..22731)
                     /locus_tag="OO339_001420"
     CDS             complement(21637..22731)
                     /locus_tag="OO339_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5926105.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22764..23762)
                     /locus_tag="OO339_001421"
     CDS             complement(22764..23762)
                     /locus_tag="OO339_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5926106.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23787..25298)
                     /locus_tag="OO339_001422"
     CDS             complement(23787..25298)
                     /locus_tag="OO339_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5926107.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25321..26303)
                     /locus_tag="OO339_001423"
                     /pseudo
     CDS             complement(25321..26303)
                     /locus_tag="OO339_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26400..29501)
                     /locus_tag="OO339_001424"
     CDS             complement(26400..29501)
                     /locus_tag="OO339_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5926108.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29508..29681)
                     /locus_tag="OO339_001425"
     CDS             complement(29508..29681)
                     /locus_tag="OO339_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926109.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29799..30992
                     /locus_tag="OO339_001426"
     CDS             29799..30992
                     /locus_tag="OO339_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5926110.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31190..32443)
                     /gene="glyA"
                     /locus_tag="OO339_001427"
     CDS             complement(31190..32443)
                     /gene="glyA"
                     /locus_tag="OO339_001427"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5926111.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32770..33960
                     /gene="hmpA"
                     /locus_tag="OO339_001428"
     CDS             32770..33960
                     /gene="hmpA"
                     /locus_tag="OO339_001428"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5926112.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34005..34343)
                     /gene="glnB"
                     /locus_tag="OO339_001429"
     CDS             complement(34005..34343)
                     /gene="glnB"
                     /locus_tag="OO339_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5926113.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34404..35738)
                     /gene="glrR"
                     /locus_tag="OO339_001430"
     CDS             complement(34404..35738)
                     /gene="glrR"
                     /locus_tag="OO339_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5926114.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35728..36441)
                     /gene="qseG"
                     /locus_tag="OO339_001431"
     CDS             complement(35728..36441)
                     /gene="qseG"
                     /locus_tag="OO339_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5926115.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36606..38033)
                     /gene="qseE"
                     /locus_tag="OO339_001432"
     CDS             complement(36606..38033)
                     /gene="qseE"
                     /locus_tag="OO339_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5926116.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38591..42478)
                     /gene="purL"
                     /locus_tag="OO339_001433"
                     /gene_synonym="purI"
     CDS             complement(38591..42478)
                     /gene="purL"
                     /locus_tag="OO339_001433"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5926117.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42737..44293
                     /gene="mltF"
                     /locus_tag="OO339_001434"
     CDS             42737..44293
                     /gene="mltF"
                     /locus_tag="OO339_001434"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5926118.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44290..44826)
                     /gene="tadA"
                     /locus_tag="OO339_001435"
     CDS             complement(44290..44826)
                     /gene="tadA"
                     /locus_tag="OO339_001435"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5926119.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44851..45486)
                     /gene="yfhb"
                     /locus_tag="OO339_001436"
     CDS             complement(44851..45486)
                     /gene="yfhb"
                     /locus_tag="OO339_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5926120.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45695..46543
                     /locus_tag="OO339_001437"
     CDS             45695..46543
                     /locus_tag="OO339_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5926121.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46882..47429)
                     /locus_tag="OO339_001438"
                     /pseudo
     CDS             complement(46882..47429)
                     /locus_tag="OO339_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11310 a        12988 c        11935 g        11196 t
ORIGIN      
        1 cgactggggg cagtaatcat gccgggtctg gctggctaac accccattat aaggggtacg
       61 aataaagtaa cggggacttg caggctggca gcgcgatgac ctgctattag aagaaggtgc
      121 agtagagctg ccggaaggag agtttaacgc cgcgtttaat cctgaagcag tggtgaaagt
      181 aaccgatgtg ttattggcaa gccatgaaaa tacggcgcaa attattgatg cccgcccggc
      241 tgcacgtttt aacgcagaag ttgatgaacc tcgcccaggt ttacgtcgcg gacatattcc
      301 cggtgcattg aacgttccgt ggacggaact ggtgcgcgaa ggcgaactaa aaacgaccga
      361 tgaactggat gcgatatttt ttggtcgcgg cgtcagctat gacaaaccga ttatcgtcag
      421 ctgcggctct ggtgtaacgg cagccgtggt tttgttagca ctcgcgacgc tggatgtgcc
      481 aaacgtgaaa ctgtacgacg gcgcatggag tgaatggggc gcgcgggcag atttaccggt
      541 tgagccagtg aaataagtat attacaggca ataaaaaacc gccgaatttg gcgttttttt
      601 attgctggtc tggttcgcgg cctttccagc aggttgactt gtgttacatg agcaacgcag
      661 gtgcttcaca gcaaaacaat actcaccagt aactctcttt ttgtcaagca aaagagagta
      721 attattgttt atttagcgta ttatcgacac cggccctttc cgccgtgttc ggtaataaaa
      781 taacctggct tattagtccg aattcagaca aatataaaca aatcctgctc aaaattaaaa
      841 attctaaccg gtaaaagata ttacttaaac atgtaaattt actttccttt aaaaaacaaa
      901 aaaccgccaa aatcaggcgg ttatttgttg ctggtccggt tcgcggcctt tccagcaggt
      961 tgtattaccg tagtaatgca agcgcgtctc agcggagaca atactcgcca gtaactctct
     1021 ttttgtcaag caaaagagag ttattattgt tctgttagtg tattatccac tgcggccctt
     1081 tccgccgtct cgcaagcggg cgctggctta aggaaaggat gttccgcgac cgttagagca
     1141 agcgcgtctc agcgcttgct atcgcggcaa tatcgccagt ggtgctgtcg tgatgcggtc
     1201 ttcgcatgga ccgcacaatg aagatacggt gcttctgtat cgtacttatt gtttctggtg
     1261 cgctgttttc ccaagtaaat aataaccgga gtctctccgg cgacaattta ctggtggtta
     1321 acaaccttca gagcagcaag taagcccgaa tgccgccctt tagggcggca tattttagat
     1381 tatccggttt tgtttaaagt cacgcaaaaa accaccccag cgacgttcat agaatggcgc
     1441 aatatgttcg gtaataaagt ggctaattcc tttttcccct tttttcacct gacaaatatc
     1501 gattggttca tcgccaggta atgtatcggt cgctacactt cccgtcgcct gaataatttc
     1561 ttcgatatca ccatcggctt caatgccaat aagtaaatta ggctgtgcct cttcgttctc
     1621 tttaattgaa caaataaaag cacgcttcac cggcttaatg gttttaaata aggtggtgag
     1681 tgaatcaatc atttgtgctg gcggctctgc gacttccgat aatatcagcg attcaccgcc
     1741 ttccaggatt tcctggctgc tcagcggatt tccctcttca ccaatcaaca aactgatttc
     1801 acgcggcata aattctttac cggtcggcag tttggcatta aggaagagcg tttcgccaag
     1861 ggtcatctca aacaatgtgc gaacgggcat tacgacaaat gcctgttcgt cttcaaccgc
     1921 ctgttgaagc gcttctaacg aggtgaaaaa aggaatgacg ctggtgccgt cttctttttc
     1981 ccagtgctgt aaatcaagcg cactatcttc aaccacagcc tcgccctgcg ccgccgtacc
     2041 aggcacccag acggtggatt ccagtagagt acggaaaaag gccgggcggt gcgccggttc
     2101 ggttgctgct ttttccagca ggtcttcaag ttcgtttttt gtttcggaca taagaaccac
     2161 aattcattca acgttcgggg cgcaaatgcc tgatgcgcta cgcttatcag gcctacaagg
     2221 gattcgcaat ttgttgaatt tgcagaattt gtaggccgga taaggcgttt acgccgcatc
     2281 cggcatatta gtttacgccg ttaacagatt agctatcgtg cgcacaccaa gtcccgtagc
     2341 gcccgcagac cactgttcaa ccggcgcttt acggtaagtc gccgagcagt cgatatgcag
     2401 ccagccttgc tgatagttct caacaaagtg cgacaggaag cccgccgccg tgctcgcgcc
     2461 tgccggatac gccgcgcttc cggtattgtt cagttcggca aagttagacg gcagctggct
     2521 gcggtggaat tccgccagcg gcagacgcca gaatggttcg ttctcttgtg aggcactcgc
     2581 cagtaaacga ccggcaagcg catcgtcaaa actgaacagc gcgtgataat cattacccag
     2641 cgcagttttc gccgccccgg tgagggtcgc cgcatcaatg atcatttccg gtttctgcgc
     2701 actggcatca atcagaccat cggcaagcac cagacgccct tcggcatcag tgttcatcac
     2761 ttcaactttt ttaccgttgc gataggtgat gatatcgccc agcttgaacg cattgccgct
     2821 aatcaggtta tccgcacagc agaggaacag cttcacgcgc ttgttcagtc cgcgcataat
     2881 ggcaaatgcc agcgccccgg taaccgttgc cgcgccgccc atgtccgact tcatcgagtc
     2941 cataaacgca gtctgtttga tgctgtagcc gccggagtca aaagtgatac ctttacctac
     3001 caggcacgcg tacaccggtg cttctttatc gccagttggg ttgtaatcca gcgccagcaa
     3061 taccggagaa cgttctgaac cgcgcccgac agtgtgcagc cccatataac cttgctcacg
     3121 cagatcttcg cctttggtga tccgataagt cacacgatcg cccgcgacgt tgctgatcag
     3181 atcaacagca cgctgtgcca gttgcgatgg tcccaattct tctgccggtg cgttgatggt
     3241 gtcacgcacc cagtcgatga tcatcaggcg gttatccagt tcctggcgct gggcatcgtc
     3301 cagatccggc cactccactt tacgcgtgcc tttcgggcct ttataacctt gccagaatgc
     3361 ccagcagcga tccgcatccc agccttcacc gcttaactga acatgcttga tgcccagacc
     3421 gtcaatcttg cgggccgcac gctggatcag ccccagatcg tctgccccgt tcaaatgcag
     3481 ggtaatgccg tcattattaa tgctgtaagt tgctttttct ccccagcgcg cgtcggcagg
     3541 ttgggtagag agggtaatct tcatcgcttc tgtcatttta gttatcctta ttcgtttaac
     3601 agcaaaacgg gctaccccca ggcagcccgt tcattcactc ggcttcgtcc agccagacta
     3661 acaaaatcgc ttcgaggatt ttctcgttgg atgcctgcgg gtcgtcgtcg aaatcttcca
     3721 gatcgcaaat ccactgatgc atatcggtga atcgaaccgt tttcggatca agatcgggat
     3781 acgcatcgta cagtgcttcg ccaatttcgc ggctatcggt ccacttaagt cccatactaa
     3841 cctctgttaa tgctcacgcg catggttgat agtgtaacgc gggatttcga ccactaaatc
     3901 ttcgtcggtg acgcgcgcct ggcagcttaa acggctttcc ggctccagtc cccaggcttt
     3961 gtccagcatg tcgtcttcct gctctgagct ttccggcagt gagtcaaaac cttcacgaac
     4021 gatgcagtgg caggtggtgc aagcacagga tttttcacag gcgtgttcaa tctcaatacc
     4081 gttacgcagc gctgcgtcga gaatggtttc accgctatta gcttccagaa cagcgccatc
     4141 agggcagagg tcctgatgag gcaaaataac aatctttggc atattaaacc tcgtccacgg
     4201 aatggccttt cagcgcacga cgaaccgact ggtccatgcg gcgagcggcg aaatcctggg
     4261 tttgtttgtc tacgttttta atcgcttgtt cgatggcgtc aacatcatcg ccctgcgcca
     4321 cttcactcag gtgagcggca gcattgtcaa tgacctgacg ctctgcggcg cttaacagcg
     4381 cggcatcagc agccagcgcg ccgtgcagac tttccagcac acgcgccgct tctacttttt
     4441 gttctgccag catacgggct tttacgtcct gctcggcata gctcattgag tctttgatca
     4501 tcgaagcgat ttcgctatcg gtcagaccgt aagacggttt gacctgaata gacgcctcaa
     4561 cgccggtgga tttctccatc gccgtcacgc tcaaaagacc gtcggcatcg acctggaacg
     4621 tcacgcgaat atgcgcaccg ccagccggta gcgccggaat accacgcagc gcaaaacgcg
     4681 ccagtgagcg gcagtcctgc accagttcgc gctcaccctg cattacatgg atagacatcg
     4741 ccgtctgacc atctttaaag gtggtgaaat cctgagcgcg ggccaccgga atagtggtat
     4801 tacgcggaat cactttctcc accaggccgc ccatcgtttc gaggcccagc gacagtggga
     4861 tcacatcaag cagcagcatt tcgctgtctg gcttgttacc caccagaata tccgcctgaa
     4921 tcgccgcgcc aatagcgacg actttatccg ggtcgatgga agtcagcggt ggacgaccga
     4981 aaaattcgcc tacccgttca cgcaccagcg gcacgcgagt agaaccgccc accatcacca
     5041 cttccagcac ttcgtcagct tctacacccg cgtctttcag cgcgcgacga caagccagta
     5101 aggttcgttt taccagtggc gcgatcagtt cattgaattg ttcacggctg atttcgccct
     5161 gccagcccgc aacgttaacg gtcacggagt ccgcatcgct cagcgcgatt ttggctgcaa
     5221 tggtggcatc cagcagttca cgctgaacgc ggttatcgct acgatcagga atgtccgcct
     5281 gctcgcgaat gtaatccgcc agcagatggt cgaaatcatc gccgccgagc gcggaatcac
     5341 cgccggttgc cagcacttca aacacgccgc gacttaagcg cagaatggaa atatcaaacg
     5401 tcccgccacc gaggtcataa acagcgatca cgccttcctg accggaatcc agcccgtagg
     5461 cgatagccgc agcagtcggt tcgttaagta agcgcagaac atgcaacccc gccagacgcg
     5521 ccgcgtcttt ggtgccctga cgctgggcat cgtcaaagta cgccggaacg gtgataacta
     5581 caccatccag ctcgcctgcc agggcttcag ttgcccgcgc cgccagtgct ttgaggatgt
     5641 ccgcagaaac gcgcaccggg ttcagtagcc ccgccgccgt ttcaatcatc ggcaggccgt
     5701 tttcgctggc ctggaattga taaggcagat gcggatagcg ttgctggata tcagccagcg
     5761 agcgccccat caggcgttta acagaactaa tggtgttggc ggtgtcaagc gctgcgttag
     5821 tacgcgcgtc ataacccacc gaatgccctt gctgttgata gtgaacaaca gatggcagca
     5881 ggtgacggcc ttcatggtcg gctaacgttt cggcctgacc gctgcgcact gtcgccacca
     5941 gcgagttggt tgtgcccagg tcaataccag ccgccagacg acgctgatgc ggcgcagcac
     6001 tcaaaccagg ttcactaatt tgtaataagg ccatgtttag cttccagaaa ttaaaaatcg
     6061 agcagttttt cttcgagttg ttcggcactg ctccgcagtt tatcgagaaa acgcagctta
     6121 cgcaccgtat ccgccgccgc gtcccacgcc tcgttgtcta actgttcaac catcaactga
     6181 tggcgggtat caaacatctt tttcacacgt ttgataaagc tttccagccg cgcttcatct
     6241 ttcgcctgtt cgatctcgtc cagctcttcg cgcagctcca actgttccat caggaacgcg
     6301 gtgtcgcgca cagtatgctg ctcgctggcg agatcaaaac cgtgtaaaga aagcaaatat
     6361 tccgcgcgca ttaacggatg acgcagcgtt tgccaggcct ggttaatggt tgcagattgc
     6421 tgtacggcgg cgagttgttc cgccaggctt ccgctggcga atttatcagg atgatactga
     6481 cgttgtagat cctgaaaacg caggctcagt gcctgggtat cgagttgata acgggcaggc
     6541 aagccaaaga gggtgaagta atccataaca ttctcagggc tgcgatccgc cgcgataacg
     6601 gcgaatcagg taaaacaaac cccacgcgca ggcgaccacg gtggggttat cggtatgcgc
     6661 atcaaacgtg gaagctttcg ccgcaaccac actcatcttt aacgttcggg ttggtgaatt
     6721 taaacccttc attcaggcct tcttttacga agtccagctg cgtaccgtcc agaaattgca
     6781 ggcttttgcc atcgaccacg actttcacgc ctttgtcttc aaacacgatg tcttccggcg
     6841 tcggttcgtc aacaaattcc agtacataag ccatacctga acatccggag gttctcacgc
     6901 ccagacgcag gccaaaccct ttgccgcggt tagccagaaa ggtatttact cgcgctgctg
     6961 cactgtcgct cagtgtaatc gacataacca aacctcaact cttattttgc ttcacgtttg
     7021 cttttatagt ccgcaatggc ggctttgatc gcgtcttctg ccagaataga acagtgaatt
     7081 ttcaccggcg gcagttcaag ttcttcagca atatcggtgt ttttgatcgc ctgcgcttcg
     7141 tcgagagact tccctttcac ccattcggtg accagggagc tggaagcgat agcggaaccg
     7201 cagccgtaag ttttaaaacg cgcgtcttca atgatacctt catcgttgac tttaatctgc
     7261 aacttcatta cgtcgccaca ggccggtgca cccaccatgc cgctgccgac gttctcgtcg
     7321 ttgttatcaa aggaacccac gttacgcgga ttctcgtaat ggtcgataac tttttcgctg
     7381 taagccatta taaattctcc tgattccgat accgattaat gatgagccca ttcgatgctg
     7441 ttcagatcca cgccctgctt gtacatttcc cacagcggag aaaggtcacg cagacgaccg
     7501 atggatttac gaactaactc gatggtgtag tcgatctctt cttcagtagt aaaacgacct
     7561 aaagagaaac ggatagagct atgtgccagc tcgtcgttca gccccagcgc gcgcagcacg
     7621 taggacggtt cgaggcttgc tgacgtacag gcggaacctg aagaaactgc gaggtctttc
     7681 agcgccataa tcagcgactc accttcaacg tagttgaagc tgacgttgag aatgttcggc
     7741 gcaccgtgtt ccaggtcacc gttcaggtaa acttcttcga tatctttgat gccgttccac
     7801 agacggttac gcaggccgcg cagacgttcc atctcggtcg ccatctcttc ttttgcgatg
     7861 cgataggctt cgcccattcc gacgatctgg tgaacaggca gagtgccgga acgcataccg
     7921 cgctcgtgac cgccgccgtg catttgcgct tcgatgcgta cacgcggttt acgacgcaca
     7981 tacagcgcac cgataccttt cgggccatag attttgtgac cggagaaaga catcaggtca
     8041 actttcaact ggctcaggtc gattggcagt ttgcccacgc tctgggttgc atcaacgtga
     8101 tagataatgc cacgagcacg gcacatttcg ccgatagccg cgatatcctg caccacgccg
     8161 atttcgttat ttacgtgcat gatggaaaca aggatggtgt cgtcacgcat cgccgcttcg
     8221 agttctttca ggtcgataat accgttacgc tgcggtgcca ggtaggtgac ttcgaaacct
     8281 tcgcgctcca gctgacggca agtatccagt accgctttgt gttcggtttt gctggtgatg
     8341 atgtgcttgc cttttttctg ataaaagttg gctgcacctt tgatcgccag gttgtcagat
     8401 tcggttgcac cagaggtaaa gacgatttca cgcggatcag cgccgaccag atcggcaatc
     8461 tgattacggg cgatatctac cgcttcttca gcctgccagc cgaaacggtg agaacgagag
     8521 gccgggttac caaaggttcc gtccatcgtc atgaactgca tcattttctc ggcaacacgc
     8581 gggtccaccg gcgtggttgc ggagtagtcg agataaatcg gtaatttcat tgctctataa
     8641 actccgtaca tcactcaatg caaggaatca ggctaccggc tggatgtacg accgtgttta
     8701 cggagtattt agcactccgg cctgattctg aattcttttt attaagcgcg taacttaacg
     8761 tcgatcgcgt cttgtgtgcg ggtgcgtggc gcgtcgtgag tatgctgacg accagacaca
     8821 tccagcactt cctggttatt aaccagttcg cctaaagtaa tgttgttgag aaaaccggtg
     8881 agacggtcgc tcaaatcacg ccacagcgcg tgggtcaggc atttatcgcc gccctggcag
     8941 ccgcctttac cctgacaacg ggtggcatct acagattcgt caacggcgct aatcacttcg
     9001 ccaacggcga tgctgctggc atctttgcct aacagataac caccgcctgg tccacgtacg
     9061 ctggaaacca gaccattttt acgcagacgg gaaaacagtt gttccagata agaaagggaa
     9121 attccctgac gttcggaaat atcagccaac ggtaccgggc ccgcttcaga gttgagcgca
     9181 acgtcaagca ttgcggtcac ggcatagcgc cctttagatg tcagtctcat gtcttacttc
     9241 acctcaaact cgcccctgcc cggggttttt tattgtaaaa gtgggggtat tgcatagcag
     9301 ggtcaagtct gacattcccg agtaaattgg tcaactattt acttgactga tttagtcggg
     9361 tatttaacct tcagtgccat ttttttatcg tggcgtttgt acctgttgtc ggatgcggtg
     9421 tgaacacctt atccgaccta cgattctgta cattgtaggc ctgataagac gcgttaagcg
     9481 tcgcatcagg caaccactgt tattcggcct tgttaccttt attctgctgc tcaatagaag
     9541 ccagaatccc gcgcaggata ttcaattcct ggctttccgg gcgcgcacgg gtaaacagac
     9601 ggcgcaattt attcatcacc tgccccggat ggttttcacg gataaaaccg gttgccagca
     9661 gcgtttgttc cagatgacca taaaaacgct ccagatcatc gaccagcgga tacggcgtct
     9721 cttcatgctc gacctgctcg ccattttcct gagttgccag ccaggccata cgcacttcat
     9781 aggcaataac ctgaaccgcc attgccaggt tcagcgaact gtattccggg tttgccgcaa
     9841 tcgcgacatg ataatggcat ttctgcaact cttcattggt caagccgacg cgctcacgac
     9901 caaacaccag cgccaccggg gtatttgccg cttcagcgac gcttttcaga ccgcattcgc
     9961 gcgggtcgag catcggccac ggcagcgtgc gggagcgtgc gctggtgccc accaccaggc
    10021 tacaaccggc taacgcttcg tcgagcgtat cgacgatgtg agcattacca atcacatcgc
    10081 tggcccctgc agccagggca atcgcctggg agtcgggttt caccagtgga ttaaccagcc
    10141 acagattggt taatcccatt gttttcatgg cacgggcaac agaacccata ttgccggtgt
    10201 gtgacgtctc caccagcaca attcgaatat tttgcagcat tgtctttctt catctaaaga
    10261 ttattcacgc atcttatcat aaaacgaaga cagatgccga tctcgctgct atactctgcg
    10321 ccgttttccc gttctttaac atccagtgag agagaccgat gcatccgatg ctgaacatcg
    10381 ccgtgcgcgc agcgcgcaag gcgggtaatt taattgccaa aaactatgaa accccggacg
    10441 ctgtagaagc gagccagaaa ggcagtaacg atttcgtgac caacgtagat aaagctgccg
    10501 aagcggtgat tatcgacacg attcgtaaat cttacccaca gcacaccatc atcaccgaag
    10561 aaagcggtga acttgaaggt actgatcagg atgttcaatg ggttatcgat ccactggatg
    10621 gtactaccaa ctttatcaaa cgtctgccgc acttcgcggt atctatcgcc gttcgtatca
    10681 aaggccgcac cgaagttgct gtggtatacg atcctatgcg taacgaactg ttcaccgcca
    10741 ctcgcggtca aggcgcacag ctgaacggct accgtctgcg cggtagcacc gctcgcgatc
    10801 tcgacggtac cattctggcg accggcttcc cgttcaaagc aaaacagtac gccactacct
    10861 acatcaacat cgtcggcaag ctgttcaacg aatgtgcaga cttccgtcgt accggttctg
    10921 cggcgctgga tctggcttac gtcgctgcgg gtcgtgttga cggtttcttt gaaatcggtc
    10981 tgcgtccgtg ggatttcgcg gcaggcgagc tgctggttcg tgaagcgggc ggcatcgtca
    11041 gcgacttcac cggtggtcat aactatatgc tgaccggtaa catcgttgct ggtaacccgc
    11101 gtgttgtgaa agccatactg gcgaacatgc gtgacgagtt aagcgacgct ctgaagcatt
    11161 aatgactcag gcgggtgata tcactcaccc gccctcgcct ttcaggcgct attccgaaat
    11221 acttcctcac cgctttactt tctttcgtca ctctcccacc atctttcccc gattaatgga
    11281 tgataaagaa gtcgcaagga aaggttatgg cactgccagt gaacaaacgc gttcccaaaa
    11341 ttctgtttat tctctttgtt gttgccttct gcgtttattt agtgccgcgc gttgccatca
    11401 acttcttcta ttatcctgac gataaaattt acggtcccga cccctggtcg gcggaatccg
    11461 tcgaatttac ggctaaggac ggtactcgtc tgcaaggctg gtttattcct tcttcgacgg
    11521 gccctgctga caacgccatc gcaaccatca ttcatgctca cggcaatgcc ggaaatatgt
    11581 ccgcccactg gccgctggtc agttggttac ctgagcgtaa tttcaacgtt tttatgtttg
    11641 attatcgcgg gtttggtaaa tcaaaaggca ctccgtccca ggccggattg ctggacgata
    11701 cgcaaagcgc catcaatgtg gtgcgccatc gcagtgatgt aaacccacaa cagctggtgc
    11761 tgttcgggca gagtattggc ggggcgaata ttcttgctgt tattggtcag ggtgatcgtg
    11821 aaggcatacg tgcggtgatc ctcgactcca catttgcctc ttatgcaacc atcgccaacc
    11881 agatgatccc cggcagtggc tacttacttg atgagagtta cagcggcgaa aattacatcg
    11941 ccagcgtcag cccgatcccg cttttactca ttcacggtaa agccgatcac gttatcccat
    12001 ggcagcacag cgaaaagttg tatagcctgg caaaagagcc aaaacggttg atcctaatcc
    12061 cggatggcga acacattgat gctttttccg atcgtcacgg caatgtttat cgcgaacaga
    12121 tggtggactt tatccttagc gcgttgaatc cgcagaacta acccatgatc gctagcacga
    12181 taatcattca caaaaccacc ttaagacatg ctaatccact ggtcagaaca gtttaaaatg
    12241 agaaaaattc tgtgacgctt gccaacattt ctgatgatta gcattccctt cgccatttcc
    12301 ctgagcaaac tttagctatt cttatcaatt atgcttatgg gagatctaca gatgatgcct
    12361 acgcttgctc caccatctgt cctttcggct ccccagcgcc gctgccagat cttgctgacg
    12421 ctctttcagc cggggttgac cgccaccatg gcaaccttca gcgagcttaa tggtgtggat
    12481 gatgatattg ccagtcttga tatcagcgaa acaggacggg agatcctgcg ctatcatcaa
    12541 ctcacactga cgactggtta tgacggtagc taccgggttg aaggtacagt gcttaaccaa
    12601 cgtttgtgtt tatttcactg gctacgacgt ggtttccgtc tgtgtccgtc atttattacc
    12661 agccaattca cccccgccct gaagagtgaa ctgaagcggc gcggaattgc gcgtaacttt
    12721 tacgacgata ccaatctaca agcgttagtg aatctctgct cccgacggct gcaaaaacgc
    12781 tttgaatcgc gcgatattca tttcctgtgt ctgtatctgc aatattgttt gctgcagcac
    12841 cacgctggaa ttacgcctca gtttaatccg ctccaacgtc gctgggcgga atcctgcctt
    12901 gaatttcagg tagcgcagga aattggacgc cactggcagc gtcgggcgct ccagcatgta
    12961 ccgcctgatg agccactgtt tatggcatta cttttttcca tgttgcgggt tcccgatcca
    13021 ctgcgggatg cgcatcagcg ggacagacaa ttgcgtcagt ctatcaaacg tctggtaaac
    13081 cattttcgtg agctgggaaa tgttcgtttt tatgatgaac aggggttatg tgatcagctt
    13141 tatatccacc tcgcccaggc gttaaatcgc agtttgtttg ccatcggtat tgataatacc
    13201 ctgccggaag agttcgccag actgtatcca cgcctggtac gcaccacccg cgcggcgctg
    13261 gccggatttg aaagtgaata cggcgtccat ctttctgatg aggaaagtgg tctggtcgcg
    13321 gtgattttcg gtgcctggct aatgcaggaa aacgatctgc atgaaaaaca gattattcta
    13381 ctgaccggga atgatagcga gcgagaagcg cagattgagc agcagttacg cgaactaacg
    13441 ttactgccgc tcaacattaa gcatatgtcg gtaaaggcat ttttgcagac aggcgctccg
    13501 cgtggcgcgg cactgattat tgcgccttat accatgccgt taccgctctt ttcaccaccg
    13561 ctgatctata cggacctgac gctgacaaca catcaacagg agcagatccg caaaatgctc
    13621 gaatcagcat gagggaacaa ctttcgggcg caaaaacatt gccggaagcg ccaccagcgc
    13681 cattacccag aacacgccgt ggcccagata ttgatacagg aaaccggcga aaacggtcat
    13741 gatagcgata ctgccgccca tcgcgacggc agagtaaacc gcctgtaaac ggatgacttc
    13801 gctaacctgg cgagcagcaa tataacgcat ggcggccagg tggcagaccg tgaaggtgcc
    13861 gcaatgcaga atttgcacca ctatcaacca cggcaacgcc gtagttgctc ccataatgcc
    13921 ccagcgcact acgccgcaaa tcgccgagat caacagcata tcgcgagcac tacaacggcg
    13981 gaaaagttta ttactcagcg caaagataat gacttccgcc accacgccca gcgaccacaa
    14041 ataccccacc gccgaggccg agtagccagc tgcctgccag taaatggcgc taaaaccgta
    14101 ataggccgca tgtgccccct gcaataaaca aacgcaggcc agaaagcgcc agttctggcg
    14161 aaccagcgcc aaccacgcag accaaccggt gctctcctgc tggcggcttg ccccttgtgg
    14221 ctgaatcgtc ggacggatga gaaagccgag cagcatggat gccacgccca acgtcaacag
    14281 cacgaggatc acccgataat caaacatagt gaccagtttg cccgtcagcg ccgagccaat
    14341 gacaaacgcc accgagcccc acagtcgcac tttgccgtaa tcaagcggga actgcttttg
    14401 ccacgtattc gccagtgcat cggtcaacgg taccagcggt gagaaaaaga ggttaaagcc
    14461 aatcatcacc agcatcagcc acgctacgtg cgcccccgcc cagaaggcga cagcaaagag
    14521 aagtggcagc agtgccagca cgcgcaaggc ggaaatcagg cgggaaggat cgctgacgcg
    14581 gggcgcgatg agcaaactcc cgagaaaacg ggcaaccaga cctgccccca ataacaggcc
    14641 gatggtttct ggcgttaaac caatcccttt aagccagacg ctccagaaag gtaaaaaaat
    14701 gccgtaacta aaaaagtatg tgaaatagcc gagcgccaac cagcgcgtgg attgcaaaac
    14761 catgagtccc tcccgtcagg aggcgttagt ctggggttaa tcgaatgttg gtgcaagttg
    14821 aaaaataaga atattcgagc ctgcttcccg atgtctgtaa cgccagttca caagcactaa
    14881 aaaactgcga tccgcgacgc atgttgcaaa aataatgcgt aattatgccg ttacgcttgc
    14941 caaacgttcc tgcacaatgg cgatgaaatc gcgtaatgcc agcttcatct cccctttctt
    15001 ccacgccatc agtaaagcaa tggaaggcac atttccggca atgggacgaa aaacaacctg
    15061 cccggtatta aaattattca tataaccggg tatcaaagtg acgcccagcc ccatgcccac
    15121 cagattcatg gtcaccagaa tattcgttgc cacctggacg atatttggct ggctgttttc
    15181 ttgcgcaaac cacgctttaa cgatcggcgc aagcgaaccg gaatacgccg gatcggtact
    15241 gacgaaattc acgccatcca gttgcgcggc ggtgatctct ttttcatgtg ctaaagggtg
    15301 atcaaccggt aacaccacca ctaatggttc gtcaaaaagc tccagataat caatctccgg
    15361 gctataaaca ggatggcgca ttaagccgac gtcgagttca ccacggcgaa ttttttcctc
    15421 ctgttgcgtg gtgattaaac tcaccagctc aatcaaggtg tctggctgtc tgagacgaaa
    15481 catcggtaat acttttggca ataaattcac ttccgccgat ggcacaaagc caatggttaa
    15541 ttgtctgtct tcctgaacaa ttttccgcgc ccgtaatttg gcattttccg cttgttcgag
    15601 gattgccagc gcgtcctgga gaaaacattc tcccgccgcc gtcaacgcga ctttgcgctt
    15661 atccctcacc agtaacggaa caccgacaca gttttcaaga tcgcggatct ggctgcttaa
    15721 cgaaggctgt gaggtatgca gtttttccgc cgcacgggta aagttcagtg cctgcgccac
    15781 tgcgacgaaa tagcgtaaat gccgtagttc catcaccttc cccttgttat cgaaaaaacg
    15841 tctcagctgg tagaaattaa ctatttcaca tattagcaac caaccagcaa catccttatg
    15901 gcacaaaaat agaaggtcaa tacatcttat ctttcaggat taaaaaatat gaccacaccc
    15961 tcagatttga acatttacca actgattgac acccaaaacg gtcgggtcac tccgcgtatt
    16021 tataccgacc cggacattta ccaactggag cttgagcgta ttttcggtcg ttgctggcta
    16081 tttctcgccc acgaaagcca gatcccaaaa cccggtgatt tctttaacac ctacatggga
    16141 gaagatgcag ttgtcgtggt gcgccagaaa gacggcagca tcaaggcgtt tctcaaccaa
    16201 tgccgccacc gggccatgcg tgtgagttat gcagattgtg gcaacagtcg cgcctttacc
    16261 tgcccgtatc acggctggtc ttatggcatt aacggcgagt tgatcgatgt accgctggaa
    16321 cctcgcgcct acccacaagg gttgtgtaaa tcccactggg ggctaaacga agttccttgt
    16381 gtggagagtt ataaaggact gatttttggc aactgggata ccagcgcacc gggcctgcat
    16441 gattacctgg gtgacattgc ctggtatctg gatggcatgc tggatcgtcg cgaaggcggc
    16501 accgaaattg tcggcggcgt acaaaagtgg gtgatcaact gtaactggaa attcccggca
    16561 gagcagttcg ccagtgacca gtatcatgct ctgttcagcc atgcttctgc cgttcaggta
    16621 ttaggggcga aagatgatgg cagcgataag cgcctcggtg atggacaaac cgcccgcccg
    16681 gtgtgggaaa ccgccaaaga tgcgctgcaa tttggtcagg acggtcacgg tagcggtttc
    16741 ttctttactg aaaaaccgga tgctaatgtc tgggtcgatg gcgcagtttc aagctattac
    16801 cgcgaaacct atgccgaagc agaacaacgt ttaggtgaag ttcgcgccct gcgcctggcg
    16861 ggtcataaca atatattccc cacgctttca tggctaaacg gcactgccac actccgcgtc
    16921 tggcatccgc gcggccctga tcaagttgaa gtgtgggcgt tctgtattac tgacaaagcc
    16981 gcctccgatg aagttaaagc cgcttttgaa aacagcgcca ctcgtgcttt tggtcctgct
    17041 ggttttctcg agcaggatga ctcggagaac tggtgtgaaa tccagaaatt gcttaaaggc
    17101 caccgcgccc gcaacagcaa actgtgtctg gaaatggggc ttggtcagga aaagcgccgc
    17161 gacgacggca ttcctggcat tactaactat atcttttcag aaactgccgc tcgtggaatg
    17221 taccaacgct gggccgatct tctgagtagc gaaagctggc aggaagtgct cgataaaacc
    17281 gccgcttacc agcaggaggt gatgaaatga gtgcgcaagt ttcactggag ttacatcacc
    17341 gcattagcca gtttctcttt cacgaagcca gtctgctgga tgactggaaa tttcgtgact
    17401 ggttggcaca gctcgacgaa gagattcgtt acaccatgcg caccacggtt aacgcgcaaa
    17461 cacgcgaccg tcgcaaaggc gtccagccgc cgacgacctg gattttcaat gacaccaaag
    17521 accagctgga gcggcgaatc gcccgtctgg aaacgggcat ggcctgggca gaagagccgc
    17581 cgtcacgcac tcgtcactta atcagcaact gccaggtaag cgaaaccgac atcccaaacg
    17641 tatttgccgt gagggtgaat tatctacttt atcgggcaca aaaagagcgc gatgaaacat
    17701 tctatgttgg aacgcgtttc gacaaagttc gccgtctgga agatgacaac tggcgcttgc
    17761 tggaacggga tatcgtcctg gatcaagcgg tgatcacttc ccataacctg agtgtactgt
    17821 tctgatgaat cgaatttatg cgtgtcccgt tgcggatgtg ccggagggtg aggctctccg
    17881 gatagatacc tcgcccgtca tcgccttgtt caacgtgggc ggcgagtttt atgccattaa
    17941 cgatcgttgc agccatggta atgcgtcgat gtcagaaggg tatctggaag atgacgccac
    18001 ggtggagtgc ccgctacacg ccgccagttt ttgcctgaaa acggggaaag cgttatgcct
    18061 gcccgccacc gatccgctca ccacttatcc agtacacgtt gaaggtggtg acattttcat
    18121 cgacttaccg gaggcgcagc catgagcgat ctgcataacg agtccatttt tattaccggc
    18181 ggcggatcgg gattagggct ggcgctggtc gagcgattta tcgaagaagg cgcgcaagtt
    18241 gccacgctgg aactgtcggc ggcaaaagtc gccagtctgc gtcagcgatt tggcgaacat
    18301 attctggcgg tggaaggtaa cgtgacctgt tatgccgatt atcaacgcgc ggtcgatcag
    18361 atcctgactc gttccggcaa gctggattgt tttatcggca atgcaggcat ctgggatcac
    18421 aatgcctcac tggttaatac tcccgcagag acgctcgaaa ccggcttcca cgagctgttt
    18481 aacgtcaacg tactcggtta cctgctgggc gcaaaagcct gcgctccggc gttaatcgcc
    18541 agtgaaggca gcatgatttt cacactgtca aatgccgcct ggtatcctgg cggcggtggc
    18601 ccgctgtaca ccaccagtaa acatgccgca accggactta ttcgccaact ggcttatgaa
    18661 ctggcaccga aagtgcgggt gaatggcgtc ggcccgtgtg gtatggccag cgacctgcgc
    18721 ggcccacagg cgctcgggca aagtgaaacc tcgataatgc agtctctgac gccggagaaa
    18781 attgccgcca ttttaccgct gcaatttttc ccgcaaccgg cggattttac ggggccgtat
    18841 gtgatgttgg catcgcggcg caataatcgc gcattaagcg gtgtgatgat caacgctgat
    18901 gcgggtttag cgattcgcgg cattcgccac gtagcggctg ggctggatct ttaaggaagc
    18961 acgatgaaag aaaaaacgat cattattgtc ggtggcgggc aagcggcggc aatggctgcg
    19021 gcctcgctac gccagcaagg gttcaccggt gagctgcatc tgttttccga tgagcgacat
    19081 cttccttatg aacgacctcc actctcgaaa tccatgttgc tggaagattc cccgcagtta
    19141 cagcaggtgt tacccgctaa ctggtggcag gaaaacaatg ttcatctgca ttccggtgta
    19201 accatcaaaa cgctgggtcg cgacacacga gagttagtgt taaccaacgg cgaaagctgg
    19261 cactgggatc agctttttat agcaaccggc gcggcagctc gaccgctgcc gttgcttgat
    19321 gcactgggag aacgctgctt taccctacac catgccggtg atgccgccag actgcgagaa
    19381 gttctgcagc ccgaacggtc agtcgtgatt gtcggtgccg gaactattgg tctggaactg
    19441 gctgccagcg ccacgcagcg cagcgcagcg cagcgcagcg cagatgtaag gtgacagtga
    19501 ttgaactggc ggcaaccgtc atgggccgta atgcaccacc gcccgtgcaa cgctatcttt
    19561 tacagcgcca tcagcaggct ggtgtgcgca ttctgctcaa taatgccatt gaacatgtgg
    19621 tcgatggtga aaaagtagaa ctgacgctgc aaagtgggga aacgcttcag gctgatgtgg
    19681 tgatttacgg tattggtatc agcgccaacg agcaactggc tcgcgaggcc aaccttgata
    19741 ctgccaatgg cattgtcatt gatgaggctt gccgcacctg cgatcccgcg atctttgccg
    19801 gtggcgatgt ggcaatcact cgtcttgata atggtgcact acaccgctgc gaaagctggg
    19861 aaaacgccaa taaccaggcg caaattgccg ctgccgcaat gttagggcta ccgctaccgc
    19921 tactgccgcc gccgtggttc tggagcgatc agtacagtga taacttacag tttattggcg
    19981 atatgcgtgg cgatgactgg ctttgtcgtg gcaacccgga aactcagaag gcgatttggt
    20041 ttaatctgca aaacggcgtg cttatcggtg cggtcacgct gaatcagggc cgtgagattc
    20101 gcccaattcg caaatggatc cagagcggca aaacgtttga tgcgaaactg ctgatagatg
    20161 agaacatcgc gcttaaatca ctgtaatcag gataattagc gaatatctca atgcctgggg
    20221 cgtggcgagg tgcaagagtg tgtattacgt ttaaatcaca ttatcttgca aagggattgg
    20281 ttatgaacac actacgttat tttgattttg gagctgcccg ccccgttttg ttattaattg
    20341 cccgtatcgc cgtggtctta attttcatta tttttggttt tcccaaaatg atgggctttg
    20401 acggtacggt ccaatatatg gcctcgttgg gcgcgccaat gccgatgctg gcagcgatta
    20461 ttgcggtagt tatggaagtg cccgccgcga tattaatcgt gcttggcttt ttcacccgtc
    20521 cgctggcggt gctgtttatt ttctacacgc tgggtacggc ggtgattggt caccattact
    20581 gggatatgac cggcgatgcg gttgggccaa atatgattaa tttctggaag aatgtcagta
    20641 tcgctggcgc gttcttgcta ttggcaatta ccggaccggg ggcaatttct ctcgatcggc
    20701 gttaggaaag atgccggatg cggcgtgaac gccttatccg gcattttata aattacagcc
    20761 actctacccg caacgacatc tctgaggttg ttgattcccc tggtgctagc gcaatgagat
    20821 cacccccttc cgggcgatga tgctcatccg gcgcatggct catcggttcc agacagaaga
    20881 aatcaaacgc atatccttta tcaaacgcag ggtcggaaac aaagatgaaa taacacggtg
    20941 caggtggcgt cgtttccatg atgatggcat aaccttcctg cggctgctcg atgcgggcct
    21001 gaccattcca tccggcaaaa ccattgttca cccactgacg cggcaacggc gcgagctggt
    21061 taaaatccag ttcctgcggt agctgctcgc aaaactcacc cgccagccac tgctcccgtt
    21121 ccagccagta accgctcgcc tgcgcctgaa tccgcgtttg tggtgacaac ggaaaataag
    21181 gatgccagcc ggtaccaaat ggcagcgtct ctgccccttg attggtgaca gagagcgtca
    21241 ccgtcagcgt atccgccgtt aaatgaaacg cctgacttac ccgatagtga tagacaccgc
    21301 tgcgatgttc atacaccaga cacaaactat catcgctgtg cgagacacat tgccactcgc
    21361 ccagccagcc atcgccgtgt agatagtgcg catcccactc aacattcggt tgcagttgat
    21421 actcacgccc ctgccagaca aaacggttgc cgctcacccg gtttgcaaat ggcaccagcg
    21481 gaaaacatga ggcatcggtt gccacaccgc tttttttacc aggacgtaag agcggcgtcg
    21541 tatcgcgcca gaagccttcg attacgccgc cctggtcaga aacgtccagc tttagcgacc
    21601 cgtgggataa ggtatagatg gtcatccgct aactccttaa tccgggaagt taatcacaac
    21661 tttcccgcat ttgccgctcg ccatcagcac ataggcatca cctgcctgtt ctagcgagaa
    21721 gcgatgggta atggcgttac gcggccacag cttccagtca gtcaaatcgt gggcgcattt
    21781 ttccatatgg aacagactgg tcacccagga gccgataatc cgccgttgat ggtgcatcag
    21841 atcagcgctg acctcgaatt ccacttttcc ggtttcacca atgtaaacca cccgccccca
    21901 gtcagcagtg gattgcagtg ccagcaagcg acctgcggca ttaccggaac aatcgagcgc
    21961 aacatccgcg ccaccgtggg taagttcagc aattatctgc ggcagacctt cggtggttgc
    22021 taaatagccg tgatccatcc cccctaactg ttttgccatc gccagacgtt ccggcagcat
    22081 atcaacgccg atgatccgtt ttgcaccgcg acctttcgcc agcatcatcg ccatcatgcc
    22141 gaccggcccc agaccgacca ccagcacgtt atcgctgccg gaaacttcgc cgcgcaaaat
    22201 cccttcatag gccgtgccaa cgccgcaact gataaacgca ccatcttcgt agctcagcgc
    22261 atccggcagg aggatcagat ctttttcttc cgccagcaga tattcggcat gaccgccgtc
    22321 acgctgccag ccgtaagccg cttttccttt gccagtacaa gagataggaa agccgcggcg
    22381 gcagttcggg caaaaaccac agccagaaat gtgatacacc agcacgcggt cgccctcttt
    22441 aaaatggcgg cagccttgcc ccatcgccac aatctgcccg cacggttcat gaccgttgat
    22501 aaatccctgg tataacggtt tatcgggtgc cgccgccgtg gcacggtgtt gatgatagat
    22561 atagtggaca tcgcttccgc aaatcccgga ggatttcatt ttgatcagta cctggttaat
    22621 ccccggcgtc ggcaccgcaa cttcccgcag atcgacggtt gaatttccta gtaaataagc
    22681 agccagcatc gttttcataa atccctcatt aacaatacga ttaattttca tccctgcagc
    22741 acccgcgcca ggggcgttaa ggtttagcgt ttcgctttac tgcttcgctg ggtcagcaag
    22801 atattcgcca gcaccgccac cacgatgatg acgccgcgta ccacctgctg gaaaaaggag
    22861 ttaataccga gcagcaccag accgttaccg attagcgtaa tcaccagcac accaagcaat
    22921 gtaccgaaca gggaaccgcg accgccggaa agtgccgtac cgccgaccac gaccgcggcg
    22981 atgacgtcaa actccagacc gtttgcggca cctgcgttac cggaaccgag gcgtgccgcc
    23041 aacaaaatgc cggtcaccgc cgctaataat cccgaaaggg taaagataag aatgcgcact
    23101 cgacgaacgt taataccgca caactgcgcc gccgtggcat taccgccaac ggcaaaaacc
    23161 gagcgcccga aggcggtttt tcggctaatg aacacaaaca gcgcaaacaa caccatcatg
    23221 atcagcgcgg atactggcac accgagaaat tgtccgccca gccagtccag cacctcgttt
    23281 tcgtcaatcg gcactggcag tgcgttggtc ataaacagcc ccattccgcg cagagcgctc
    23341 cacagcccca gtgtggcaac gaaacttggt acgttaaaca cgccgcgcag caccccggcg
    23401 agcgttccca tcagcgcgcc taacagcaac accagcagac aagcaatcgc caacggaact
    23461 tcaaattgca gcaaaaatgc caggcacacc gagacaaaag ccaccatcgg cccaacgctg
    23521 acatcaattt caccggagat aataatcagc gtcatcgccc aggcggcaat cccaatggtg
    23581 gcggcatcgc gcagcacgtt catctggtta ttcaatgaga taaagccagg cgcgttcagg
    23641 gagaagacca gataaagaat ggcaatcacc accagcaaac cgatctcatt aatatggcga
    23701 ctgacaaatt gtttgagcga gacgctcttg ccctgcggca atggtaatga cgaagcatac
    23761 atcttcaggt tcctcggtaa aattcatcag tgcacagaca gaatggcgga catcagctca
    23821 tccacattga ccggcgagtg aaactcctgc gagaacgtgc cgtgctgtaa taacaggatg
    23881 cggtcacaca ccagcggcaa ttcctccact tcactggaga taaacaccac gctttttcct
    23941 tcggcagcca gctcacggac aatacggtaa atctgctgtt tggcttcgat atcgacgccg
    24001 cgcgttggct cgtcgagcaa caaaatctgg ctggcggcat agacccaacg accgatcacc
    24061 actttttgct gattgccacc agaaagcgtg ccgatgggtg tttcgctact ggcagccttg
    24121 accgtcatcc gctgcatcac ctcttcggtc aggcggcgga tggtggacca ttgcagcaca
    24181 ccgttggtgc tgattttttg ccgattggtc agcactgtat tttcgtcaac gcctagccag
    24241 ggaatgatcc ccgcttcttt gcggttttct ggcgtatagc caatgccgcg tttcagcatg
    24301 tcgccgtaat cggggcgcat gattttctcg ccgttgataa caatttcgcc ctgttcatac
    24361 gtctccagcc caacaatcgc cttcagcaat tcactgcgcc ctgcccccag caggccagca
    24421 atgccaagca cttcgccacg acgtagcgta aagctgatat cctccagctt gggcttatgg
    24481 cgtaacgcac ggacttccag tacggcctga tccacaattt cctgaggggc aaccggcgca
    24541 atatcaacgt gatcacgccc tagcattagc gacacaatat gatgcgtgga ggtgttttcg
    24601 agcatcacat cgcccgccac ctgaccatcg cgcataacgg tggcatagga ggcaatgcgg
    24661 cggatttctt ccatccggtg gctgacataa atcaccgcca cgcccagtgc cgacatcttt
    24721 ttcaccgcac tgatcaccag ttcaacttct gcactggcaa gcgagctggt gggttcatca
    24781 agaatgacca cgcgcggctc gcccttcatc acccgtgcaa tttccaccag ttgcttttgc
    24841 gccgggctta gcgttgaaac aagttgttca ggactaacgt caacgcccag cgcctgtaag
    24901 caacgttggg catcctgcgc catttgcagg taatcaatca tgccgttgcg gcgaggccat
    24961 tgaccaaggc agaggttttc tgccaccgtc agcccttcca ccagacttaa ttcctgataa
    25021 accgcacgaa cccccagttc agcggcacgg cgagtcaacg tagcttcgtc accttccagt
    25081 cgcgtctcgc caatccagat atcaccgcta tccgggcgtt cgctgccggt aagcattcga
    25141 atgagagtcg atttgcccgc gccgttttta cctaacagcg cacgaacttc gcctttattg
    25201 agcgtgaagt taacgttatc cagcgcaacg acgccggggt aacgcttatt tcctgccacc
    25261 acttttgcta ccgggactgc ctctgttgcc gtgaacatag ggacctctgc gaatcagcga
    25321 ttagggcaga ccatcaacgt gcgttgccag ccactgttta ccatcttccg ttttggtata
    25381 gagatcgatt ggcacctgaa tcactttttc accgtcggct tgtttattga taaccttcaa
    25441 tgtttgcgcg aaaacagcat tgcccatttt cttaccggaa atatccacta ccgctttcag
    25501 cacctgattg ttttccagct cctgagcaat ttcggttgtc atatccgaac cgaaaacagc
    25561 aatttttccg gcctgatttt gattacgtac cgcttttacc gcgccgagtg tcgcaccgcc
    25621 cgactccccc ataatggcgt tgagatccgg cgtggagata atcagttttt caccaacgga
    25681 aatcgcttta tctaaaacag tcccttcctg gttagcgaca atttgcgcgc cgggaacgcg
    25741 ggattttaat acttcttcaa atcctttacg tcgctgcaca caaacttcaa aggcttcgca
    25801 attgatgacg gcaattttcg gctggtcaat tttattggca ataaaataat cggcggcagc
    25861 gttacccagt ttttaccaaa ttccagtgga tcgccgacca gatacgccga gacatattta
    25921 tcgacaccct tttgattaat acaggtgttg tagcaaatca ccggaatacc cgcttcactg
    25981 gcgcgacgaa cggtacggct actgccattt tcagacactg ccgataaaat aatggcatcg
    26041 acattacgcg ccacgagggt atcaacaaag gtactttctt tcgaaatatc cccctgggcg
    26101 ttagtttcaa ttaactgtac ctgtactgaa gaatcttttg ccgcatcctg aacgccctga
    26161 cgcactccag cgtaatatcc ctgggtatca aggtatattg caccaatggt catttcttta
    26221 tccgcagccc tggcaaatag tgcgctacct aatagcgtag ccattaataa tagattacgg
    26281 gttgttctca tttttttagg catagtgcct cctgtagggt ttttattaac aacggcttat
    26341 tctaattatt ttgtgatgag cggcagcgcg tgccgctcaa atattacagt taatagggtt
    26401 tagtgaacgt agttaaaaat aaaaggcatc acagtggtga ataatgccgc tttcggccag
    26461 gccggattaa tcgccgtcaa ttcagcgcga gcagactcat attgcgcaac ctccccgagt
    26521 cccgcggacg ccagcatcgc caccatcagg catttttctt tatgctgctg ttgtaaatcg
    26581 ccatacagcg ataacaggtc aggttgtgaa acagcaaaga aatccgcctc aatactggtt
    26641 ttcgccatct cttgcgccca ctgtttcatt tcactaaaca gttgctgtgc ggtttgctgt
    26701 tcacccagca gtcgcagcgc cattccttgc cagaagagat aatcaaccgg ctgatcgttg
    26761 taataactgt ggatgttaat ggtgcgatcg ccggtcgccg ccagacgtaa acaacgcatc
    26821 gcttcagttt catcgccctg cgcgttggcg catatcgcct gccagaacca gatgtcgtta
    26881 tcagtttgcc ccggtaaacg gccttcgctt aaattctccg gataatacag cgcggcatga
    26941 agcagttcgc tggcctgctg cggctctctg gcatcaagat gctgccaggc gcgtaataac
    27001 tggttgagga taaactgact ggtgaccttc ccttccccgc cttcccacgg gtggaattta
    27061 cgcgtggcga gaatgtccgc cgctttgtct gcctgacccg tgagatgcca caaattgagc
    27121 agttctgcgg tcatgtcgtc gcgtttcagc gcaatttcca gattattttc cagacgcgcc
    27181 agtcgtttct ccggtgtggc tccacttaac ttatccagca aatcccgttc gaaaagcaga
    27241 cgtgcatcct gcggcgcaag ctgataagca ttatcaagat aacgcgcggc cagctcataa
    27301 tcgtgtttct tattccacgc atggatcgct aacccgcgcc agccgtcggc aaactccggc
    27361 gacatctcta cgcaacgttg ccataaggca atggctttgt tgtagctacg tttgttgtag
    27421 tagaagcagg ccagtaaatg gcgagcaaac caacactctt caatactctc cagcgccgcc
    27481 acttcttcca gcgtattcgg gaaacggaca aactgcggga agacatcaat ggcttttgcg
    27541 accagttcgc cacgttcggc tttcggcagc aagctggctt gcaggtaaag cggcagcgtg
    27601 cgctggcagt ccagtgcgtt cagcatctct gccgccaggg tgggcattcc ccagttaatc
    27661 agttgcccgg cggtcatcag agcgttaacg tcgcgtccct gacacagacc gcgccactgc
    27721 gcgagggctg attcgctacg accatcgaac cagttcagcc accacagagt ggcgttcagc
    27781 ggataatcac gcagcagttt ctcgcgctgc aaacgcgcgt tgtcctgacg accacttaac
    27841 accagcagca gattatgcaa gcaaagcact tcctgattgg ttgggcaggc acgaagactt
    27901 tgttggcaaa aatccagacc cgcgtcgaag ttatcattac gcgccgccag tcgtgccaga
    27961 ccataatagc cgcctgcttt actattgccg ctccagaccg cacgccagaa atcctcttcg
    28021 gcttgttgat attgtccctg acgttcgtaa gcactggcgc gaatcaaact tgcttgtcca
    28081 cactgcggtt ttttgttcag cgcatgtgca cgtttcagcg cctgactggc atacgccacc
    28141 gcttgcggga aatctgcgcg gttatattcc agcatcgcca gcgccaggtt acagcgataa
    28201 tccagcgggt ccagcgccac gccgcgcagg tagtaatcga acggtgaacg gctggcgtga
    28261 tgatattgct ccagatgctg accgataaac caggcttcat ctgtactggt aatgtcttgt
    28321 gctgccagtg gcgctttggc gacgtccggc aacggcaact cctgcggctg atgttcctga
    28381 taactcagta caatattgcc gtcggcatcg gagagttcaa tggtcagtct gtcaggattg
    28441 ataccgtgca acacgccctg gatggcggtc gctggtgtca gggccacggc atcatcgagc
    28501 aacgcgttgc atttgccgat ttcgcggatc gccagacggt atccgttcaa cggagagatg
    28561 gcatacagcc cccactcaat cccccgctca ctacgctgga gttttatcac cgcatcgcgg
    28621 gaggcatttt gcaccatgcc cagagaatga taaggcagga aatactgctc gaaacgcttc
    28681 tcttcgtaag catcaagcca ggtaaaatca ggctggttat cggcaaaaat accggtcatc
    28741 agttcgatat acgggccgtt attgtcggtc agactcttat cccacgcctg gccaaattca
    28801 ctgtgtcccc aactccactg ttttttacct ggcgcaatat ggtggttggc aacgtgcagc
    28861 aaaccgccat cttcatcgtg acaccacgcg ccgacaaaat cgtactgcga tttttcggcc
    28921 atatatgagg ttggaacagg cacattttta tagcgagaaa tgtccactcc ggcggagtag
    28981 tccactttgt agtaagtgcc ggtggcgatg gggaaagcgg agacggcccg tttgccgtga
    29041 tcaaacaccg cagtcacatc cggtgggaaa acgctctgat gcccttcacc ccctttcact
    29101 gccgggttga cccaccacaa gaaatgacgc ggcgtggcgt tgccgttata gacgcggctg
    29161 gcgatttcca gtgccgcccg gtcagggcgc agggtgaaac ctgtcatcac ctgtaaacca
    29221 tgcatcggct ccgtttcgcc gacccacacc gtctgtgcac cgtcttcatg ggcttcgagg
    29281 gtgaaatcaa cgggcataaa ggtggtcggg cgatggtgtt gcggccagtt aaactcaatc
    29341 ccgccggaga tccacggtcc cagcatcccc accagcgcag gtttaatgac ttcattgtga
    29401 tagacaaaat cgcgctgttt cactttatcc catgcgcgat gcacccgacc gcccagttcc
    29461 ggcaggatca tcactttgat gtagtcgttt tccagccaca ccgcctgcta ggatttcagg
    29521 gttttctgct cgctcagcgt atcggtcacg ccgtagggat aaaccgcgcc ggacgatccc
    29581 tgataaacgc gattttccag gaacatggga tgtatatcct gcggcccggt ttcataggtc
    29641 gggatctcaa cgcgctcttg ccacactttt actggagtca tggtgccctc aatattaaca
    29701 agacatactg aattaaaaga tttgtggcag tgtattgaac aatctggcaa tgttttcgcg
    29761 gaataatcac gcaattaact aaacaaggtt tagtgaagat gagagcctgc attaacaatc
    29821 aacagattcg ccaccataac aaatgcgtga ttctggaact gctgtaccgg caaaagcgcg
    29881 ccaataaatc aacgctggcc cggctggcgc aaatttcgat tccggcagtc agtaatattt
    29941 tgcaggaact ggaaagcgaa aaacgggtgg tgaatattga cgatgaaagc cagacgcgcg
    30001 ggcatagtag cggtacatgg ctgattgcgc cggaaggtga ctggacgctg tgcctgaacg
    30061 tgacgcccac cagtattgag tgtcaggttg ctaatgcttg tttaagtccg aaaggcgaat
    30121 ttgagtattt acagattgat gtaccgacgc cgcaggcgct gctgtccgaa atcgaaaaat
    30181 gctggcatcg ccaccgtaaa ttgtggccgg accgcaccat caatctggcg ctggcaatcc
    30241 acgggcaggt tgatccggtg accggcgtat cgcaaaccat gccgcaagcg ccgtggacaa
    30301 cgccagttga ggtgaagtat ctgctggaag agaagctggg cattcgggtg ctggtcgata
    30361 atgactgcgt gatgctggcg ctggcggaga aatggcaaaa taattcgcag gaacgggatt
    30421 tctgcgtgat caacgttgat tacggcattg gctcgtcgtt cgtgattaac gagcaaattt
    30481 atcgcggcag tttgtatggt agcggacaga ttggtcacac catcgttaat ccggatggag
    30541 tcgtctgcga ctgtggacgt tacggcagcc tggaaaccgt cgcctcgtta agtgcattaa
    30601 aaaaacaggc gcgggtatgg ctaaaatcac aaccggttaa tactcagctt gagcctgaaa
    30661 aactgacaac cgcgcagtta atcgctgcct ggcagagtgg agaaccgtgg atcaccagct
    30721 gggttgatcg ctctgccaat gccattggtt tgagtctgta taacttcctc aacatcctca
    30781 atattaatca gatttggttg tacggtcgca gttgtgcctt tggtgagaac tggcttaata
    30841 ctattattcg ccagacagga tttaacccgt tcgaccgcga cgaaggaccg agcgtgaaag
    30901 cgacgcaaat tggcttcggg caattaagcc gcgcacaaca ggtgctggga attggctatt
    30961 tgtatgttga ggcgcagtta cgacagattt gatggcgcga taacgtagaa aggcttcccg
    31021 aaggaagcct tgatgatcat aaacgaaaaa ttgcctgatg cgctacgctt atcaggccta
    31081 cacggagatt gcaatatatt gaatttgcaa gattttgtag gcctgagaag gtgttcatgc
    31141 cgcatccaac atgaacaacg agcacattgt cagcaaatca ccgtttcgct tatgcgtaaa
    31201 ccgggtaacg tgcgcagatg tcgagaactt tacctttgat gcgctcgata acggcttcat
    31261 cattgatgct gtccagcacg tcacacatcc agccagccag ttctttcgct tcggcttctt
    31321 taaagccgcg acgggtaatc gccggagtac ctacacgaat accggaggtc acaaacgggc
    31381 tcttcggatc gttcggtacg ctgtttttgt tgacggtgat gttagcacgg cccagagcgg
    31441 cgtctgcttc tttaccggtc aggtttttat caaccagatc aaccaggaac aggtggttat
    31501 cagtgccgcc ggaaaccact ttgtagccgc gctcgaggaa cacttctacc atcgctttag
    31561 cgtttttcgc gacctgctgc tggtaagttt tgaactcagg ctccatcgct tctttcagag
    31621 caaccgcttt accggcgatt acgtgcatca acggaccgcc ctgaccacca gggaaaacgg
    31681 cagagttcag ttttttgtac agctcttcgc taccaccttt cgccaagatc aggccgccgc
    31741 gcggacccgc cagggttttg tgagtggtgg tagtaacaac gtgagcatga ggaaccgggt
    31801 tcgggtagac gccagcagca accaggcccg caacgtgcgc catatcaacg aacagataag
    31861 caccgatgct gtcagcgatt tcacgcattt tcgcccagtc aaccacgccg gaatatgcag
    31921 agaaaccacc gataatcatt ttcggcttgt gttctttggc ttgtttttcc agatcggcgt
    31981 aatcgatatg accggtagca tcgataccgt aaggaacgat gttgtacagt ttaccggaga
    32041 agttaaccgg agaaccgtga gtcaggtgac cgccatgcgc caggttcata cccagaacgg
    32101 tatcacctgg ttccagcagc gcggtgtaga ccgcaaagtt agcctgggag ccggagtgcg
    32161 gctggacgtt agcgtagtca gcgccgaaca gttctttcgc acgatcgatc gccagttgtt
    32221 caacgatatc aacatactcg caaccgccgt agtagcgttt gcccggataa ccttcagcat
    32281 atttgttggt cagctgagaa ccctgcgcct gcattacgcg cgggctggtg tagttttcgg
    32341 aggcgatcag ttcgatgtgc tcttcctgac gtactttttc ctgctccata gcctgccaca
    32401 gttcggcatc ataatcggca atgttcattt cacgctttaa catccgcatc tcctgactca
    32461 gctaacaata aaatttttgg cctttatagg cggtcctgtt ggacaacggc gaacagtata
    32521 accgaatcat tgtgcgataa caggtcttga caaaggaatt tacgcaaacg attaccttca
    32581 ggctacgcaa ggctttggag aataaagagc ttgcaaccgg aaacggattt cttttcaggt
    32641 ttgtgatgca aatttttcac ttcatcacat tctttctgaa aaacaccaaa gaaccattta
    32701 cattgcaggg ctatttttta taagatgcat ttgagataca tcaattaaga tgcaaaaaag
    32761 gaagaccata tgcttgacgc tcaaaccatc gctacagtaa aagccaccat ccctttactg
    32821 gtggaaacgg ggccaaagtt aaccgcccat ttctacgacc gtatgtttac tcataaccca
    32881 gaactcaaag aaatttttaa catgagtaac cagcgtaatg gcgatcaacg tgaagccctg
    32941 tttaacgcta ttgccgccta cgccagtaat attgaaaacc tgcctgcgct gctgccagcg
    33001 gtagaaaaaa tcgcgcagaa gcacaccagc ttccagatca aaccggaaca gtacaacatc
    33061 gtcggtgaac acctgttggc aacgctggac gaaatgttca gcccggggca ggaagtgctg
    33121 gacgcgtggg gtaaagccta tggtgtactg gctaatgtat ttatcaatcg cgaggcggaa
    33181 atctataacg aaaacgccag caaagccggt ggttgggaag gtactcgcga tttccgcatt
    33241 gtggctaaaa caccgcgcag cgcgcttatc accagcttcg aactggagcc ggtcgacggt
    33301 ggcgcagtgg cagaataccg tccggggcaa tatctcggcg tctggctgaa gccggaaggt
    33361 ttcccgcatc aggaaattcg tcagtactct ttgactcgca aaccggatgg caaaggctat
    33421 cgtatcgcgg tgaaacgcga agagggtggg caggtatcca actggttgca caatcacgcc
    33481 aatgttggcg atgtcgtgaa actggtcgct ccggcaggtg atttctttat ggctgtcgca
    33541 gatgacacac cagtgacgtt aatctctacc ggtgttggtc aaacgccaat gctggcaatg
    33601 ctcgacacgc tggcaaaagc aggccacaca gcacaagtga actggttcca tgcggcagaa
    33661 aatggcgaag ttcacgcctt tgctgatgaa gttaaggaac tggggcagtc actgccgcgc
    33721 tttaccgcgc acacctggta tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt
    33781 gatagcgaag gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg
    33841 cagttctatc tctgcggccc ggttggcttc atgcagttta ccgcgaaaca gttagtggat
    33901 ctgggcgtga agcaggaaaa cattcattac gaatgctttg gcccgcataa ggtgctgtaa
    33961 tttgatgttg ccggatggaa acatccggca acccttgacg cggtttaaat tgccgcgtcg
    34021 tcctcttcac cggtacggat gcgaatgacc cgagccacgt caaagacgaa gattttaccg
    34081 tcaccgattt tgccggtttg cgccgtgcga ataatggtat cgacacaggt atcgacaatg
    34141 tcgtccggta cgacaatctc aattttcact ttcggcagaa aatccaccat atactccgcg
    34201 ccgcggtaca gctcggtatg gcctttctgg cgaccaaagc ctttcacttc ggtcaccgtc
    34261 atgccggtaa taccgacttc ggccagtgct tcgcggacat cgtccagctt gaagggtttt
    34321 ataatcgcat caatcttttt catgctattc cttgaaaagg ttgcctgtct tttgatctgc
    34381 taaacgtaac acataacgcc aattcattcc ttgaaatcgt ttgcatccag ctcgtgtcgg
    34441 gaaagcagtt tataaaattc tgtccggttg cgccccgcca ttctcgccgc gtgggtgacg
    34501 ttgcctttgg tgatttgcag cagcttacgc aagtagttga gttcaaaatg attacgtgcc
    34561 tcaacaaagg ttggcagcgc cgtattttca ccctccagcg cctgctccac cagcgcatca
    34621 ctaatcaccg gagatgaggt cagcgccacg cactgttcaa tcacgttgac caactggcgc
    34681 acattacccg gccagctcgc ggtcatcagg cgtttcatcg catcggtaga gaacgcgcgg
    34741 acaaacggtt tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag tagcggaatg
    34801 tcttctgtgc gctccgccag tgccggaatt ttcaggctga caacgttgag gcggtaatag
    34861 agatcttcgc ggaattcccc gcgcgccatc gcttttgaca gatcacggtg agtggcagaa
    34921 ataatccgca cattgatatc aatatcgcgg ttactgccca gcgggcgcac tttacgctcc
    34981 tgcaacacgc gcagcagttt gacctgtaac ggcgcaggca tatcgccaat ttcatcgaga
    35041 aatagcgttc cgccttccgc cgcctggaat aaaccttcgc gattgctgac agcgccagta
    35101 aacgcgccac gcgcatgacc aaacagctcc gactccagca attgctcggg caatgcgcca
    35161 cagttaatag caataaatgg tttgctgttg cgcgggctgg cgttgtggat agcctgggcg
    35221 aaaatctctt tcccggtgcc gctctgaccg ttaatcaaaa cgctgacgtc tgattgcgcc
    35281 accagccgcg cctgttccag caaacgcagc atcagcgggc tgcgggtgac aattgcctcg
    35341 cgccagcgtt catcggtggc tggcgcggat tgctccagcg catcgtcaat tgcctgatat
    35401 agcgcatctt tatcgacagg cttggtgagg aaactaaaaa cgccctgctg tgttgcagca
    35461 acggcatcgg ggatagaacc atgcgcagta agaataatta ctggcattcc cggctgcact
    35521 ttctggattt cagcaaatag ctgcataccg tccatttcat ccatccgcag gtcgctgata
    35581 actaaatcta ctttttcgcg attcagtacc cgtaatcctt cagcgccact ttccgccgtg
    35641 accacactgt agccttcgct ggtcaggcgc aggccaagca gtttcagcaa tcccggatcg
    35701 tcatcgacca ataataaatg cgcaggttta tggctcatca ggagtgacct catgggtgga
    35761 tggcgcgggc ttttcactct cgtgcggcgt atccggcgag aaatttccag ccggtttgcg
    35821 ggtcgagagc tggcgttcaa tatcggtcag gttttccagc ttgcgggtgg tgagttccag
    35881 ttgctgctgt agaacgtgat gttgctggcg caatgtatcc agctcgctgt cgctggactg
    35941 ctggagttta ctgtaacgtt ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc
    36001 gcgccagagt tgatacagtg ggcgaacctg tgccgggatc tcggtactta acgcttcaat
    36061 acgcgcgacc agctggcggc gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc
    36121 ttgtttaaag gtattttgcc agctgccgtc gtcatattgg cgagcttgct gacgcgactg
    36181 cgcaggcatt aaacgatcag cacaatccat cgcccgcagc cagtaaagcg gattggtttc
    36241 ggttgattta ccttgcagcg cccagatgtc gctacattca gtagaaagat agtcagccag
    36301 ttgataaacc ggaatttttt cttctgctgg cgtatcaatg gctggcttat tgtgattctg
    36361 cacgcaaccc agcaatgcca gacatggcag ccctgccagc cacagccgtc ggggcaataa
    36421 tcgttgaaaa atgtgtcgca tattcaccag acttaaagcc tatcccagtg ggcgtaattg
    36481 ttgcagacag tctggacatg gacagcgcgg agaaaccggg gcgtacatat cgtacgtgaa
    36541 gatttcgagc actacccggg gccgaactga caaataaaat agcctgatag gataggctgt
    36601 tagcattatt tcgtgttttt cgacgacggt aattcaatgc ggaaacaaac gtcttgcccg
    36661 ctctcgtcga ccagatacag ttccccttgc atacggcgaa tacaatccct ggcaatgctt
    36721 aatcccagac cgctgccctt caccgcccct tttcgctggt ggcttccctg aaaaaagggt
    36781 tcgaagatca tggcgcgttc ctcttgcgga atgggcgtgc ctgtattgat gacatcaata
    36841 taaacccgcg caccatgtaa actgctgcga aggcaaatgt taccggattc agccccgtag
    36901 tgcaccgcat tggagtaaag attatccagt acgctcatca gcagcattgg ctccgccagg
    36961 caagctgttg ctttgagatc gacgtcggta tgcatcattt tagcccgtgc gggcaggcta
    37021 tgagcagaaa ccactgtctc caccagcggt gctaattcaa cattctccag ttccaccgca
    37081 ctgtccgcct gtttacggtt gtaatcaagc agttgttcga tcagtttttg caaattgcag
    37141 ctgctgctat caagaatgct caccacctct ttttgctctg gcgtaagcgg cccgacaacc
    37201 tgatcagcca gtaattcagt gccctcgcgc atactcgcca gtggcgtttt taattcatga
    37261 gataaatgtc ttaaaaattg atggcgttgg gattccagcc atgacaggcg ctcacttaac
    37321 caaagaatac gttgcccaac cgagcgtaac tcgctcggtc cacagaacga aacgctattg
    37381 cccagagaac gcccttcccc cagacggttg atcatgcgct cgatattttt caccggcccg
    37441 ataatcatcc gcgtgaaaag tagcaccatc accagactca ccagaaatag caccagcgat
    37501 tgccaaccaa aatattgccc acgttcggcg atttcacgct gaagttgctg cccacgagag
    37561 aacaccactg tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa ggcttcaaga
    37621 cgcgcggcgg cggcagcatc gggaccactg ttgttacact gaagttgggc cagattgttc
    37681 aaatcctgac gcaatgcctg gtaaagttta tcgtctggca gtacgcctgc gtgggcatcg
    37741 agcatttcgc tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc
    37801 agcacgcaat actgacggta gctacgctcc atctccagcg ccgcgttggt catcgcttca
    37861 ctacgccggg catcaataag cgtagtgcgg ttaaccagcg ccgcctgatc gctaagcgca
    37921 ttcaggcttt gccaggcttg ccatgccagc accaacaggg gtagtagaat tagcaaaaat
    37981 gccagcatta ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt caaggtgtta
    38041 ctctcgtcag acgcgaatag cctgatgcta accgagggga agttcagata caacaaagcc
    38101 gggaattacc cggctttgtt atggaataag gcggtgccta actcgacgtt tcgcccgatg
    38161 gttgatatag ctacgctgat atcagaagtt ggacggcagg caccttgttg tgcgtcattc
    38221 gaattttatg tagcacgtcc cgaaggggct gacataagtc ggtgaatgag ccactgctta
    38281 ctattatgca gtaactgtgc caataaagaa aatagtttgg taacgtactg attatacgtt
    38341 gctttgaggg atttatggct tcttcgctat ttgaatgtaa atcagccact gtgtcgctaa
    38401 aaagagacaa cttaagataa acttattaga ttgcaataaa atcaatcatt taagtgtcgc
    38461 cagaaagcga cacggtaaac cacccattgt cgtgatttac agacacaaaa aagctcccgg
    38521 agttgggagc ttatgatagt ggttggtgct taatgccgca tccgggctgc aaaaccaatg
    38581 aactgatgac ttaccccaac tgcttacgcg cattgcggaa aatgcgcatc catgggccat
    38641 cctcgcccca gttttccgga tgccaggagt tgctgacagt acggaaaaca cgttccgggt
    38701 gcggcatcat aatggtgact cgaccacttt cagtcgtaac cgccgtaata ccgttcggtg
    38761 aaccgttcgg gttagccggg taggtttcag tgactttgcc gaagttatcg acatagcgca
    38821 gtgccaccag ccctttgctt tccagtgccg ccagatgcgc cgcatcacgc acttccacgc
    38881 gcccttcacc atgagagaca gcaatcggca tttgcgagcc caccatcccc tgcaacagca
    38941 gagacgggct ttgggttact tcaaccaggc tgaaacgcgc ttcaaagcga tcggaggtgt
    39001 tacgcacaaa acgtggccac aactcactcc ccgggatcag ttcacgcagg ttagacatca
    39061 tctggcaacc gttacatacc cccagggcca gcgtttgcgg acgatggaag aaggttgcaa
    39121 actcatcgcg tacacggtca ttgaacagga tagacttcgc ccaaccttca ccggcaccca
    39181 gcacatcacc gtaggagaaa cctccgcacg cgaccagggc gtgaaaatcc tccagtcccg
    39241 tacgtccagt cagcaggtcg ctcatatgca cgtcgatagc atcaaagccc ccacgttgga
    39301 aagctgccgc catttcaaca tgcgagttca cgccctgctc acgcagtacg gcaactttcg
    39361 gacgtgcgcc agtggcaata tacggtgctg ccacatcttc gttgatatcg aacgacagtt
    39421 ttacattcag gcccggatcg gcgtcgttag atttcgcctg atgctcctga tcggcacact
    39481 ccgggttgtc acgcaggcgc tgcatctgcc aggtagtttc tgcccaccag acacgcaacg
    39541 tagtgcggct ttcgctgaat acagtctgcc cgttggcggt aatcacaaaa cggtcaccgg
    39601 aaaccgcctg cccaacataa tggacgcaat ccgcaagccc atgctgtgcc agtacggact
    39661 cgaccgcttc acggtcagcg gcacgaacct gaatcaccgc acccagttct tcgttaaaca
    39721 acgccgccag gcgatcgtca cccagagagg cgatatccgc gtcaatgcca caatgacctg
    39781 caaaggccat ttccgccagc gttaccagca ggcctccatc agagcggtcg tgatacgcca
    39841 gcagcttacg ctgtgcaacc agcgcctgaa tcgcgtcata gaagcctttc agttggacaa
    39901 catcgcgtac atctgccggt ttgtcgccaa gctgacgata aacctgcgcc agcgccgttg
    39961 cgcccagcgc gttattgcct ttgcccaaat caatcagcag cagtgcgtta tcttcggtac
    40021 aaagctgcgg cgtgatggtg tgacgtacat cttccacgcg ggcaaatgca gaaatcacca
    40081 gcgacagcgg cgacgtcatt tcgcgctctt cgttaccttc ctgccagcgg gttttcatcg
    40141 acatagagtc tttacccacc gggatcgtca ggcccagcgc cggacaaagt tcttcaccca
    40201 cggctttaac ggcttcatac aggcccgcat cttcaccagg gtggcctgcc gccgccatcc
    40261 agttggcgga aagtttaatg cgtttgatat cgccaatctg tgttgcggca atattggtta
    40321 acgcttcacc gaccgccaga cgggcagagg cggcgaaatc cagcagcgca accggcgcac
    40381 gctcgccaat cgccatcgct tcaccgtagt agctgtcgag gctggcggta gtgaccgcgc
    40441 agttagcgac cggcacctgc cacggcccca ccatctgatc gcgcgccacc atgccggtta
    40501 cgctgcggtc accgatggtc accaggaaag ttttctccgc cacggtcggc agatgcagta
    40561 cgcgtttcac cgcgtctgca atggtgatcc cttcacggac cagcgcatcg cccttcgctt
    40621 tcagcgtttg tacatcgcgg gtcatcttcg gcgttttacc aagcaggacg tccagcggca
    40681 gatcgatcgg ctgattatca aaatgacgat cgtgcagaga aagatgcagt tcttcggtcg
    40741 cttcaccaat caccgcgtag ggtgcgcgct cacgcttaca cagttcgtca aacagcggta
    40801 attgatcggc agcaaccgcc agcacgtagc gttcctggga ttcgttacac cagatttcca
    40861 gcgggctcat gcccggttcg tcgcttagaa tatcgcgcag ttcaaattta ccgccgcgtc
    40921 cgccgtcgct caccagctcc ggcatcgcgt tagaaagacc gcccgcaccg acgtcgtgga
    40981 taaacagaat tgggttagca tcaccaagct gccagcaacg gtcgatcact tcctggcaac
    41041 gacgctccat ctccgggttg tcgcgctgta cggaagcaaa gtcgaggtcg gcatcagact
    41101 gaccagacgc catggaagac gccgcgccgc cgccaagacc gatgttcatt gccgggccgc
    41161 cgagaacgac cagcttcgca ccgacgttga tctcgccttt ttgtacgtga tcggcgcgaa
    41221 tgttgccgat cccgcccgcc agcatgatcg gtttgtgata accgcgcagc tcttcgccat
    41281 tgtggctgtt cactttttct tcataagtac ggaagtagcc gttcagtgcc ggacgaccaa
    41341 attcgttgtt aaacgccgca ccgcccagcg ggccttcggt catgatgtcc agcgcggtaa
    41401 caatgcgctc aggcttaccg aaatcttctt cccacggctg ttcgaagcca ggaattcgca
    41461 ggttggatac ggagaaacca accaaaccag ctttcggctt cgcgccgcgc ccggtggcac
    41521 cttcatcgcg gatttcaccg ccggaaccgg tcgccgcgcc cggccacgga gaaatcgccg
    41581 tcgggtggtt gtgagtttcg actttcatca ggatgtgcgc cggttcctga tggaaattgt
    41641 agcggcccgt ttcgtggtca gcaaagtagc ggcccacttc agaaccttcc attacggcgg
    41701 cgttatcttt ataagcagag agaacgtgat ctggcgtggt ttcgaaagta tttttgatca
    41761 tcctgaacag cgatttcggc tgttgttcac cgtcgataat ccagtcggcg ttaaaaattt
    41821 tgtggcggca gtgctcagag ttcgcctgag cgaacatata cagctcgatg tcgttcgggt
    41881 tacgaccaag ctttgtgaaa gcatcctgca gatagtcaat ttcatcttcc gccagagcca
    41941 agccaagacg caggttagcg tcgatcagcg cctgacggcc ctgccccagc aaatcaacgc
    42001 ttgtgaccgg agtcggttga tggtgggcaa acaactgctc tgcatcatct aaatcaaaaa
    42061 agaccgtttc catcatgcgg tcgtgcagtt cagcggtaac ctgctgccat tgttcattgg
    42121 tcagcgtacc ggcttctata tagtaagcaa cgccgcgctc aaggcggttt acctgttgta
    42181 gcccgcagtt atgggcaata tcggtcgctt tcgaagacca gggagagatg gtgccaggac
    42241 gcggggtcac cagcaggagt ttgccttgcg gggcgtggct ggcgagtgcc ggaccatatt
    42301 tcagcaggcg ttcaagttgt gcgtgctcat catcgtttaa cggcgcattg aggtcagcaa
    42361 aatggacata ctcggcgtaa atattgtgaa ccgggagcct ggcagcctga aaacgtgcca
    42421 gcagtttgtt gattcggaat gccgacagtg caggcgaacc acgcagaatt tccatcataa
    42481 gtctctcgtc ttctaagctt tcggtgtacc caaggggggg gaaacgggcg tcattataaa
    42541 gaatctgatg cgctgacgaa accgtttgcg tggaaataaa atcaccatcg tgaattagca
    42601 acgcgtgccg ccaatggctg taataagttg ccatctggcg caggtttacg caaaatgccg
    42661 ctcatttatg agtaaacctt tcactattat tacgtttttt caagctggga cgcgcacgac
    42721 acagagaatt aactaattga aaaaattaaa gattaattat ctgttcatcg gcattctggc
    42781 actgctgctc gcggtcgctc tctggccatc cattccctgg tttggtaaag ccgacaaccg
    42841 tatcgccgcc attcaagcgc ggggagagtt gcgtgtgagc accattcata ctcccctgac
    42901 ttataacgaa atcaacggga aaccttttgg cctggattac gaactggcga aacagtttgc
    42961 cgattacctc ggcgtaaaac tgaaagtgac cgtgcggcag aatatcagcc agctgtttga
    43021 cgaccttgat aatggtaacg ccgacctgct ggcggcagga ctggtctata acagtgagcg
    43081 ggtaaaaaat tatcagcctg gccctaccta ttattccgtg tcacaacaac tggtttataa
    43141 agtgggtcag tatcgcccac gtacgctggg caacctgacg gcggagcaac tcaccgttgc
    43201 accgggtcat gtggtagtta acgatctcca gaccctgaaa gaaacaaaat tcccggaatt
    43261 aagctggaag gtagacgaca aaaaaggctc tgcggaatta atggaagatg tcatcgaagg
    43321 aaaactcgat tacaccattg ctgattctgt cgccatcagc ctgtttcagc gcgttcaccc
    43381 ggagctcgcc gtagcgctcg atatcaccga tgaacaaccg gtgacttggt ttagcccgtt
    43441 agatggcgat aatacccttt ccgccgccct gctcgacttc ttcaacgaaa tgaatgaaga
    43501 cggtacgctg gcacgcattg aagagaaata cctggggcat ggcgatgatt ttgattacgt
    43561 cgatacgcgc acatttttac gcgccgtcga tgcggtactg ccgcagttaa agcccctgtt
    43621 tgagaaatac gccgaagaaa ttgactggcg tttgctggcc gctattgctt atcaggaatc
    43681 gcactgggat gcacaggcca cttcaccgac gggtgtgcgc ggcatgatga tgttaaccaa
    43741 aaataccgcg caaagcctcg gcattacgga tcgtaccgat gccgaacaga gcatcagcgg
    43801 tggcgtgcgt tatttgcagg atatgatgag taaagtgccg gaaagtgtgc cggagaacga
    43861 acggatctgg tttgccctcg ctgcgtacaa tatgggctat gcgcatatgc tggatgcccg
    43921 cgccctgacg gcaaaaacca aagggaatcc tgacagttgg gctgacgtaa aacagcgtct
    43981 gcctttactt agccagaaac cctattacag caagctgact tacggctacg ctcgtgggca
    44041 tgaagcctac gcttatgtcg aaaatattcg taagtatcag attagcctgg tgggttatct
    44101 gcaagagaaa gagaagcagg ctacagaagc ggcgatgcaa ctggcgcagg attatccggc
    44161 ggtatcgcct acggagttgg gcaaagagaa atttcctttt ctctcgtttc tttcccagtc
    44221 gtcatcaaac tatttgaccc attctccctc tctgctgttt tccaggaaag ggagtgaaga
    44281 gaaacaaaat taatccgtcg aggattgcgc ttttttctgc gctttaattt cctggcggcg
    44341 catgcgaaag aagtcactga gcaacgccgc gcactcatcc gccagtattc cttccgtaat
    44401 ttccactcgg tgattcatac ccggatgatg cagcacatcc attaaagatc ccgcagcgcc
    44461 agttttcgcg tcacgcgcac caaagaccac gcgaccaatg cgactgtgga tcatcgctcc
    44521 ggcacacatt acacatggtt caagcgtgac atacaacgtg gcgtcgatca gacgataatt
    44581 ttgcatcacc agaccaccct gccgcagggc catgatttct gcatgtgcgg tgggatcatg
    44641 gcgaccaatc gggcggttcc agccttcgcc gattacccga ttgttatgca ctaataccgc
    44701 gccgaccggc acttcccgct catcccaggc acgtttcgcc agcgtcagcg cgtgacgcat
    44761 ccaatattcg tggctaaatt cgacttcaga caaaaagaaa actccggtta taaaagcgcg
    44821 gcgcattata cacggacgct atgctttact ctattccagt tgctggagtt caccgcgcgg
    44881 ggttactcgc caacgatgct gacagaaata aagcagcgga ttgtcctgat tactgtcgct
    44941 atagccactg tacagccgca gcggcgtgcc gattttgcgc tccagttgtg cgaccttttc
    45001 atgtcccaga caacgcatcg tcaataccca accaccatag ccacgctgaa tttggctggc
    45061 gataagatta acccgcggca gccagggcgt atcgaaataa accgcttcaa ccagcggctg
    45121 cggagagccg gtaatcaacc agatatcagc atcggaactt aacaggtagg tggttaatcg
    45181 ctcctgaacc agcggaaagg cggtaacatt gtcgcgaaac cagcgcacga aatcggcctg
    45241 caacgtctgt aaacgtgctt cgctgtgacc aaaagtgcac ccccacagaa gcagactcat
    45301 cggccagcgt gccgcacgac cttttatcaa taacgcaatg gctataatcg gtaacaacgg
    45361 caggacaagt aacgcattca gcggttggcg acgcagtaaa tagcgcagaa aactgccgaa
    45421 catatcctgc tgatgcaatg ttccatctaa gtcaaaaaac accacacgac gctcgtgagt
    45481 tgccaaacca tactcctctg gactgaaaca tcctgattaa ttttcgctac tacatagcct
    45541 aacagataga tcatcacttt tccggcaaca atcgaccgca tcatggctaa ctggaatttt
    45601 taattcatgg caattagcgg caatggaata taaaattcac tcgcgtgtgt ctcatattgc
    45661 ctgttgtcgc cactatttca tcgcgaggaa agatatgaac ggcttacttc gtatccgtca
    45721 gcgttaccaa gggcttgccc agagtgataa aaaactggcg gattatctgc tgctacaacc
    45781 tgatacggcg cgccatttaa gctctcagca actggccaac gaagccggag tcagtcagtc
    45841 cagcgtcgtg aagttcgcgc aaaaactcgg ctataaaggt tttccggcgc ttaagttggc
    45901 gttgagtgaa gcgctggcaa gccagccgga atcgccctcc gtgcccattc ataaccaaat
    45961 ccgcggtgat gatccgttac ggctggtcgg cgaaaaactg attaaagaaa ataccgccgc
    46021 tatgtacgca acgctaaacg ttaatagtga agagaaactg catgaatgcg taacaatgtt
    46081 gcgctctgcg cggcggataa ttctgaccgg tattggcgct tcgggtctgg tggcgcaaaa
    46141 ctttgcctgg aagctgatga agattggctt caatgctgcc gcactgcgcg atatgcatgc
    46201 gctactcgca acagtacagg cgtcgtcccc tgacgatctg ttattagcca tttcctacac
    46261 cggtgtacga cgcgagttaa acctggcggc agatgagatg ctgcgagtgg gcggaaaagt
    46321 gctggcgatt accggcttta ctccgaatgc cctgcaacag cgtgcttctc attgcctgta
    46381 taccattgcc gaagaacaag cgacaaacag tgcttcaatc tctgcttgtc acgctcaggg
    46441 aatgttaacg gatttgctgt tcattgcgct gattcagcag gatctggaac tggcaccaga
    46501 acgtattcgt catagtgaag cgctggtgaa aaaactggtc tgagtaaaga atgcgcgtat
    46561 aatgcccgcc cggtttgtgt tgttttgaga gtttccttat ggcgctgtta atcactaaaa
    46621 aatgcatcaa ttgtgatatg tgtgaaatca aataaaaaca catttatttt catagtgata
    46681 cagcaatgtg ttattgaatg tgttgttgga tttatggtgc aaaaaaattt tgcataagtt
    46741 cagcaacaca aagaacacca ctttctttaa cgtcctccgc gccctaaagg acggggcttt
    46801 acggcgcaaa aggcctgtga catacaacat taacttttca gtcaaccgta gcacgttctt
    46861 gtatacgccg tgtctgcggt tttatataac cctggctgga aacctcttat acaaagtcga
    46921 ttcgccaaca tcatagatga tcgccacctt ctggcgagaa actcctgatg caattagtcg
    46981 cccggcctgc gcccattatt ctggtgaaag tttgggacgg cgaccgccaa ttcgtccctg
    47041 tgcgcgagca gcttccagtc ctgcttttgt tcgttcaaca atcagttcac gttccatttc
    47101 agccagggca cccatcacat ggaagaaaaa gcgtcccatt ggtgtgctgg tatcaattga
    47161 atcagtcaga ctacgaaagt tgatgcctcg ttcgcgcaac gcctccacca gcacgacaag
    47221 atgccgcata ctgcgcccca gccgatccag cttccagaca accagtgtgt cgcctgccga
    47281 taatgtcttg agcagttttt tcagccccgg tctttcggac tttgtaccac ttatcttgtc
    47341 ttcaaaaatc agctcgcatc ctgcacagtt caacgcatta cgttgtagat cggtattctg
    47401 gtcatttgtt gatacgcgta catagccaa
//
LOCUS       DAJTRH010000024        45649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTRH010000024 DAJTRH010000000
VERSION     DAJTRH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            95..2248
                     /locus_tag="OO339_001439"
     CDS             95..2248
                     /locus_tag="OO339_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5926122.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="OO339_001440"
     CDS             2301..4031
                     /locus_tag="OO339_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926123.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAISKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO339_001441"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO339_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5926124.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="OO339_001442"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="OO339_001442"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5926125.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="OO339_001443"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="OO339_001443"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5926126.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO339_001444"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO339_001444"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5926127.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="OO339_001445"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="OO339_001445"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5926128.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="OO339_001446"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="OO339_001446"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5926129.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="OO339_001447"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="OO339_001447"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5926130.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="OO339_001448"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="OO339_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5926131.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO339_001449"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO339_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5926132.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO339_001450"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO339_001450"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5926133.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO339_001451"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO339_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5926134.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO339_001452"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO339_001452"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5926135.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO339_001453"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO339_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5926136.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO339_001454"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO339_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5926137.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="OO339_001455"
     CDS             16289..16828
                     /locus_tag="OO339_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5926138.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="OO339_001456"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="OO339_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5926139.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="OO339_001457"
     CDS             complement(18187..18534)
                     /locus_tag="OO339_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5926140.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO339_001458"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO339_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5926141.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO339_001459"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO339_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5926142.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO339_001460"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO339_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5926143.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO339_001461"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO339_001461"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5926144.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO339_001462"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO339_001462"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5926145.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="OO339_001463"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="OO339_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO339_001464"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO339_001464"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5926146.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO339_001465"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO339_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5926147.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO339_001466"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO339_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5926148.1"
                     /translation="MTHQLRSRDTIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO339_001467"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO339_001467"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5926149.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO339_001468"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO339_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5926150.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="OO339_001469"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="OO339_001469"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5926151.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="OO339_001470"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="OO339_001470"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5926152.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="OO339_001471"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="OO339_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5926153.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSEKAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO339_001472"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO339_001472"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5926154.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="OO339_001473"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="OO339_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5926155.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="OO339_001474"
     CDS             complement(39992..40447)
                     /locus_tag="OO339_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926156.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="OO339_001475"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="OO339_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO339_001476"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO339_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5926157.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="OO339_001477"
     CDS             complement(41290..41967)
                     /locus_tag="OO339_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5926158.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO339_001478"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO339_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5926159.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO339_001479"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO339_001479"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5926160.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="OO339_001480"
     CDS             complement(43173..43631)
                     /locus_tag="OO339_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5926161.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="OO339_001481"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="OO339_001481"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5926162.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..>45649)
                     /gene="adrA"
                     /locus_tag="OO339_001482"
                     /gene_synonym="dgcC"
     CDS             complement(44577..>45649)
                     /gene="adrA"
                     /locus_tag="OO339_001482"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5926163.1"
                     /translation="AAHGEEPPLTPQNEHQRSGLRFARRVRLPRAVGLAGMFLPIAST
                     LVSHPLPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNLKTDAVLAGMWVGVMG
                     VNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVSFNSAPLEWW
                     LSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHWETMLRNEFD
                     NCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRGSDVIGRFGGD
                     EFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVGVAPLNPQMSHYREWL
                     KSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11859 a        11918 c        11176 g        10696 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aatatcaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg gtatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaaaggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcag cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccg
//
LOCUS       DAJTRH010000025        42780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTRH010000025 DAJTRH010000000
VERSION     DAJTRH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(78..614)
                     /gene="ssb1"
                     /locus_tag="OO339_001483"
     CDS             complement(78..614)
                     /gene="ssb1"
                     /locus_tag="OO339_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5926164.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            868..3690
                     /gene="uvrA"
                     /locus_tag="OO339_001484"
     CDS             868..3690
                     /gene="uvrA"
                     /locus_tag="OO339_001484"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5926165.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3725..4081)
                     /locus_tag="OO339_001485"
     CDS             complement(3725..4081)
                     /locus_tag="OO339_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5926166.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4085..4501)
                     /locus_tag="OO339_001486"
     CDS             complement(4085..4501)
                     /locus_tag="OO339_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5926167.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4612..5325)
                     /gene="aphA"
                     /locus_tag="OO339_001487"
     CDS             complement(4612..5325)
                     /gene="aphA"
                     /locus_tag="OO339_001487"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5926168.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5727..5931
                     /locus_tag="OO339_001488"
                     /pseudo
     CDS             5727..5931
                     /locus_tag="OO339_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6434..7627)
                     /gene="tyrB"
                     /locus_tag="OO339_001489"
     CDS             complement(6434..7627)
                     /gene="tyrB"
                     /locus_tag="OO339_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5926169.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7880..8959)
                     /gene="alr"
                     /locus_tag="OO339_001490"
     CDS             complement(7880..8959)
                     /gene="alr"
                     /locus_tag="OO339_001490"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5926170.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9012..10427)
                     /gene="dnaB"
                     /locus_tag="OO339_001491"
     CDS             complement(9012..10427)
                     /gene="dnaB"
                     /locus_tag="OO339_001491"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5926171.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10510..11493
                     /locus_tag="OO339_001492"
     CDS             10510..11493
                     /locus_tag="OO339_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5926172.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11659..11901)
                     /gene="pspG"
                     /locus_tag="OO339_001493"
     CDS             complement(11659..11901)
                     /gene="pspG"
                     /locus_tag="OO339_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5926173.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12035..13072)
                     /gene="dusA"
                     /locus_tag="OO339_001494"
     CDS             complement(12035..13072)
                     /gene="dusA"
                     /locus_tag="OO339_001494"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5926174.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13435..14420)
                     /locus_tag="OO339_001495"
                     /pseudo
     CDS             complement(13435..14420)
                     /locus_tag="OO339_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14738..15253
                     /gene="zur"
                     /locus_tag="OO339_001496"
     CDS             14738..15253
                     /gene="zur"
                     /locus_tag="OO339_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5926175.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15295..15504)
                     /locus_tag="OO339_001497"
     CDS             complement(15295..15504)
                     /locus_tag="OO339_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5926176.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15620..16945)
                     /gene="dinF"
                     /locus_tag="OO339_001498"
     CDS             complement(15620..16945)
                     /gene="dinF"
                     /locus_tag="OO339_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5926177.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17018..17626)
                     /gene="lexA"
                     /locus_tag="OO339_001499"
     CDS             complement(17018..17626)
                     /gene="lexA"
                     /locus_tag="OO339_001499"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5926178.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17736..18104)
                     /gene="dgkA"
                     /locus_tag="OO339_001500"
     CDS             complement(17736..18104)
                     /gene="dgkA"
                     /locus_tag="OO339_001500"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5926179.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18275..20698
                     /gene="plsB"
                     /locus_tag="OO339_001501"
     CDS             18275..20698
                     /gene="plsB"
                     /locus_tag="OO339_001501"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5926180.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20853..21725)
                     /gene="ubiA"
                     /locus_tag="OO339_001502"
     CDS             complement(20853..21725)
                     /gene="ubiA"
                     /locus_tag="OO339_001502"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5926181.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21738..22235)
                     /gene="ubiC"
                     /locus_tag="OO339_001503"
     CDS             complement(21738..22235)
                     /gene="ubiC"
                     /locus_tag="OO339_001503"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5926182.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22458..24038)
                     /locus_tag="OO339_001504"
                     /pseudo
     CDS             complement(22458..24038)
                     /locus_tag="OO339_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24267..25187)
                     /gene="malM"
                     /locus_tag="OO339_001505"
     CDS             complement(24267..25187)
                     /gene="malM"
                     /locus_tag="OO339_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5926183.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25339..26679)
                     /gene="lamB"
                     /locus_tag="OO339_001506"
     CDS             complement(25339..26679)
                     /gene="lamB"
                     /locus_tag="OO339_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5926184.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26751..27866)
                     /gene="malK"
                     /locus_tag="OO339_001507"
     CDS             complement(26751..27866)
                     /gene="malK"
                     /locus_tag="OO339_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5926185.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28231..29421
                     /gene="malE"
                     /locus_tag="OO339_001508"
     CDS             28231..29421
                     /gene="malE"
                     /locus_tag="OO339_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5926186.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29575..31119
                     /gene="malF"
                     /locus_tag="OO339_001509"
     CDS             29575..31119
                     /gene="malF"
                     /locus_tag="OO339_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5926187.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31134..32024
                     /gene="malG"
                     /locus_tag="OO339_001510"
     CDS             31134..32024
                     /gene="malG"
                     /locus_tag="OO339_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5926188.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32395..33870
                     /gene="xylE"
                     /locus_tag="OO339_001511"
     CDS             32395..33870
                     /gene="xylE"
                     /locus_tag="OO339_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5926189.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33914..34324)
                     /gene="psiE"
                     /locus_tag="OO339_001512"
     CDS             complement(33914..34324)
                     /gene="psiE"
                     /locus_tag="OO339_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5926190.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34450..34593)
                     /locus_tag="OO339_001513"
     CDS             complement(34450..34593)
                     /locus_tag="OO339_001513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926191.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34594..34872
                     /locus_tag="OO339_001514"
     CDS             34594..34872
                     /locus_tag="OO339_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926192.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34919..37015)
                     /locus_tag="OO339_001515"
     CDS             complement(34919..37015)
                     /locus_tag="OO339_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5926193.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37015..37752)
                     /locus_tag="OO339_001516"
     CDS             complement(37015..37752)
                     /locus_tag="OO339_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5926194.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37749..38387)
                     /locus_tag="OO339_001517"
     CDS             complement(37749..38387)
                     /locus_tag="OO339_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5926195.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38501..38743)
                     /locus_tag="OO339_001518"
     CDS             complement(38501..38743)
                     /locus_tag="OO339_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926196.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39206..40855)
                     /gene="pgi"
                     /locus_tag="OO339_001519"
     CDS             complement(39206..40855)
                     /gene="pgi"
                     /locus_tag="OO339_001519"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5926197.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41380..42729
                     /gene="lysC"
                     /locus_tag="OO339_001520"
     CDS             41380..42729
                     /gene="lysC"
                     /locus_tag="OO339_001520"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5926198.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10641 a        11010 c        10502 g        10627 t
ORIGIN      
        1 gaggcttctt ttttatggct ggcagatgct ttaatcatcc accttaaaac aatataacct
       61 attgttttaa tgacaaatca gaacggaatg tcatcatcaa agtccatcgg cggctcgtta
      121 gacggcgctg ccggagcgga ctgctgcggg cgagactgcg cgccgccgct gaactgattg
      181 ccaccctgcg gctgctgagg ctgaccccaa ccgccctgcg gctgaccacc accgatattg
      241 ccacctgccg gagcgccacc accctgacga ccacccagca tctgcatggt gccgccaacg
      301 ttcaccacga cttctgtggt gtagcgatcc tgaccggatt gatcggtcca tttacgggta
      361 cgcagctgac cttcgatata aacctgagaa cctttacgca gatattcgct cgccacttct
      421 gccagtttgc cgaacagcac aacgcggtgc cattcagtct gttctttcat ctcgccggtc
      481 gctttatcac gccaggattc ggaagtagcc agcgtaatgt tggcaactgc gccaccattt
      541 ggcatgtagc gtacttccgg gtcctgaccc agattaccaa cgagaataac cttgtttacg
      601 cctctgctgg ccatgttcgt gtctcctgaa aaaaatcgtt ctgaataagt gtaaacgcgc
      661 gattgtacca ttaccaatag cgcttttact atgttgtgac ctcggttccg ggaaacaaac
      721 ctggccagac attgttacac aacactccgg ataatgcatt ccaatactgt atattcattc
      781 aggtcaattt gtgtcataat taaccgtttg tgatcgccgg tagcaccatg ccaccgggca
      841 aaaaagcgtt taatccggga aaggtgaatg gataagatcg aagttcgggg cgcccgcacc
      901 cataatctca aaaacatcaa cctcgttatc ccccgcgaca agctcattgt cgtgaccggg
      961 ctttcgggtt ctggcaaatc ctcgctcgct ttcgacacct tatatgccga agggcagcgc
     1021 cgttacgttg aatccctttc cgcctacgcg cggcagtttc tgtcactgat ggaaaagccg
     1081 gacgtcgatc atattgaggg gctttctcct gccatctcaa ttgagcagaa atcgacgtct
     1141 cataacccgc gttctacggt ggggacaatc accgaaatcc acgactattt gcgtttgttg
     1201 ttcgcccgcg tcggcgaacc gcgctgtccg gaccacgacg tcccgctggc ggcgcaaacc
     1261 gtcagccaga tggtggataa cgtgctgtcg cagccggaag gcaagcgtct gatgctgctc
     1321 gcgccaatca ttaaagagcg caaaggcgaa cacaccaaaa cgctggagaa cctggcaagc
     1381 cagggttaca tccgtgctcg tattgatggc gaagtctgcg atctttccga tccgccgaaa
     1441 ctggaactgc aaaagaaaca taccattgaa gtggtggttg atcgcttcaa ggtgcgtgac
     1501 gatcttaccc aacgtcttgc ggagtcgttt gaaaccgcgc tggaactttc cggtggtaca
     1561 gcggtagtgg cggatatgga cgacccgaaa gcggaagagc tgctgttctc cgccaacttc
     1621 gcctgcccaa tttgcggcta cagtatgcgt gaactggagc cgcgactgtt ttcgtttaac
     1681 aacccggcag gtgcctgccc gacctgtgac ggccttggcg tacagcaata tttcgatcct
     1741 gaccgcgtga tccaaaaccc cgagctgtca ctggctggcg gtgcgatccg tggctgggat
     1801 cgccgcaact tctattactt ccagatgctg aaatcgctgg cagatcacta taagttcgac
     1861 gtcgaagcgc cgtggggcag cctgagcgcg aacgtgcata aagtggtgtt gtacggttct
     1921 ggcaaagaaa acattgaatt caaatacatg aacgatcgtg gcgatacctc catccgtcgt
     1981 catccgttcg aaggcgtgct gcacaatatg gagcgccgtt ataaagagac agaatccagt
     2041 gcggtacgtg aagaattagc caagtttatc agcaatcgcc catgcgccag ctgcgaaggg
     2101 acgcgtctgc gtcgggaagc gcgccacgtt tatgtcgaga atacgccgct gcctgctatc
     2161 tccgacatga gcatcggtca tgcgatggaa ttcttcaaca atctcaaact cgctggtcag
     2221 cgggcgaaga ttgcggaaaa aattcttaaa gagatcggcg atcgcctgaa attcctcgta
     2281 aacgtcggcc tgaattacct gacactttcc cgctcggcag aaacactttc cggcggtgaa
     2341 gcccagcgta tccgtctggc gagccagatt ggtgcgggcc tggttggcgt tatgtacgtg
     2401 ctggacgagc cgtctatcgg cctgcaccag cgcgataacg agcgcctgtt gggtacgctt
     2461 atccatctgc gcgatctcgg taataccgtg attgtggtgg agcatgacga agacgcaatt
     2521 cgcgccgctg accatgtgat cgatatcggt cctggtgcgg gtgttcacgg cggtgaagtg
     2581 gtcgcagaag gtccgctgga agcgattatg gcggtgcctg aatcgttgac cgggcagtac
     2641 atgagcggta aacgcaagat tgaagtgccg aagaaacgcg ttccggcgaa tccagaaaaa
     2701 gtgctgaagc tgacaggcgc acgcggcaac aacctgaagg acgtgacgct cacgctgcca
     2761 gtcggtctgt ttacctgcat cacaggggtt tcaggttccg gtaaatcgac gctgattaac
     2821 gacacactgt tcccgattgc ccaacgccag ttgaatggtg cgaccatcgc cgaaccggca
     2881 ccgtatcgcg atattcaggg actggagcat ttcgataaag tgatcgatat cgaccaaagc
     2941 ccaattggtc gtactccacg ttctaacccg gcgacctata ccggcgtgtt tacgcctgtg
     3001 cgcgaactgt ttgcgggcgt accggaatcc cgtgcgcgtg gttatacgcc aggacgtttc
     3061 agctttaacg tccgtggcgg gcgctgcgaa gcctgtcagg gcgacggtgt gatcaaagtg
     3121 gagatgcact tcctgccgga tatctacgtg ccgtgcgatc agtgcaaagg taaacgctat
     3181 aaccgtgaaa cgctggaaat taagtacaaa ggcaaaacca tccacgaagt gctggatatg
     3241 accatcgaag aggcgcgtga gttctttgat gcggtgccag ctctggcgcg taagctgcaa
     3301 acgttgatgg acgttggtct gacgtacatt cgcctggggc agtccgcaac cacactttct
     3361 ggtggtgaag cccagcgcgt gaagctggcg cgtgagctgt caaaacgcgg caccgggcag
     3421 acgctgtata ttctcgacga gccgaccacc ggtctgcact tcgccgatat tcagcaactc
     3481 ctcgacgtgc tgcataaact gcgcgatcag ggcaatacca ttgtggtgat tgagcacaat
     3541 ctcgacgtga tcaaaaccgc tgactggatt gtcgacctgg gaccagaagg cggcagtggc
     3601 ggcggcgaaa tcctcgtctc cggtacgcca gaaaccgtcg cggagtgcga agcttcgcat
     3661 acggcacgct tcctcaagcc gatgctgtaa tcgttaaggc cgctttctga gcggcctttt
     3721 cctttcagag ttgtaccagc aatttacgct tttcttccgg tagtaaattc actgcctgct
     3781 gataagacgc atccaccaga taatagattt gcgaatccgg cagcgaaccg tcgagataca
     3841 cggtgctcca gtgcgcttta ttcagatggc ggctaggacg cacatcgctg tgctgctgac
     3901 gtagcagctc cgccagctcc gggctggttt tcagcgaaac agctgggcga ttttctactt
     3961 ctttcaccat cgcaaacagt acatcttcca ctttgatctg cgtcgctttc cagtcgttat
     4021 gcacgctctg ttctgcgctt ggttttgcca tgcaatattg tagcaactcc gaaatggtca
     4081 ttttttactc cccttgtagt gtcgcgatga tgcgacgcga tccgccgtgg atgcgatgtt
     4141 cccccagcca gatgccttgc caagtgccgg tctgaatacg ccctttatgt accggcaata
     4201 caagcgatgt tcccagcatt gaggatttga tatgagaagg catatcgtct gctccctcat
     4261 agtcatgctc ataatttccg ttgtcgggaa cggtgcggag gaaaaaacac tccatgtcgt
     4321 ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga ggcggaggta tgttgcagca
     4381 acagatgcag taagccgatg ttaacgcgcg gcatatcagc cagctgattc agaatttcat
     4441 ccgttaccag atgaaaccca cgagatttgg cgctaagcgt gagcgtcttt tgataccaca
     4501 tgtgctgctc cttgataaaa ctctcttaat cagtttgcag caagacagcg aaaggataaa
     4561 ggtgtgatta aaaaaacagc attcgggaga gcatcactct ctcccgctct gtcagtattc
     4621 tgaattgacg atcacctctt caccaaacgc acccgcttgt ggcaagggtt tgtaggtaga
     4681 gttggaggcg cgcagaatgc ggataccacg agcgccgaca tcgcgtgcgg cggtaatatc
     4741 gttatcggaa tcgccataaa aaattcggat atttttatcc tgcagccatt gcgattttgt
     4801 attttgccct ggtttatcgc ccgcaaagat taccggattc atgttggtgg caggaatatg
     4861 aaaattatcc gccagcgttt ttgaaaccgt ttctgttttc gtcgggctac gaccagtcac
     4921 aaagaagatc gcgtcaccgc ggcgtacatg catatcaatc agctggcgag cgacctcttt
     4981 tggaatgctg aattcatccc agccattatt cattttttcc cagaacacag gatttttcag
     5041 ataatcttcg ctttctggcg agaaggtttt tttgccgcgc cagaaccccg gactggaaaa
     5101 aagtaccgtg tcatcgatat caaaccccac cgccattggc ggacgccctg cgaggctatt
     5161 ttcaatttgt gcgaccgaaa cccaatgaat tggtgcctgt tcagcaagcc tggcaacgtt
     5221 agtcccaggg ttaagcggtg aaggagatga ggccagggca acagcggaac tgtttagagc
     5281 gaacaataag caaacggcac tgattgcctg tgtgatcttg cgcatatttt tccctaaata
     5341 gtcagtatgt tgaaactttt tattgtgaga tttgttgcaa aaaccatctg accataacgc
     5401 cagctgccgg ggaaaggaag gattatctgc ggtttttgtg atgaaaagca ggatgattat
     5461 atttaaccca gtaaatacgt tcctgtcttt ttttgtataa aaaatgttta ttactcgatg
     5521 taaggataca ttaattttac gtttattatg atgaatcctc ggcaaatgag cgaaatttga
     5581 tagaacattt atctgctgta cgatttaatg aacttaatga tacagcatta caaaaacaat
     5641 cgaagtttat aaagatgatt tctgattgac cagcccccta ccctacaatg gtgattaatt
     5701 ttattactcg cgatggagat gatgatatga atatatcata tgtgaatagc aacaaaacga
     5761 catctttacc tgttgagcta gatgcactaa ataataaaga tatctcttat gccaaagatt
     5821 tctttttata catagaaaca cagttaaaaa ttgcaaaaga tttttgtaga cctggagaag
     5881 aagtatcaag ttctattgca agtaaagttt ttcacgcatt tattgattta gttaacaaaa
     5941 tcaggggtaa gaaagacttt atgtatattt gcacgctttg ctgttttgcc gaggaggtta
     6001 aaggtgatta ttctcattac aggacctttt tatttgatat tggtaatcaa tataaggtta
     6061 aacttacaca aagcggaaaa aagagttctc tttaacatta gaatttaacg atactataat
     6121 tgaatctcag aaagtcacag gcaataaggc aaagcatatt cttgaggata tagaaatatt
     6181 ctatcgtaac aaacccgata cttattatta aatttaataa tggttagatg tgcagaaatt
     6241 attactccac acgaaaaaat agggaaagga attccagata attgggataa acttaacaat
     6301 aaaagaactg acaacgtttt attatctctc acgtagaaca acggcatcaa aaaaatcatc
     6361 tacaaagaaa tagtgaaagc gacgacagtt taatttcact gcaggctggg taactccagc
     6421 ctgctttcct gcattacatc accgcagcaa acgcctttgc cacacgttgt acatttgccg
     6481 tatttaaccc ggcgacacac atgcgaccgc tggcgatgag atagacacca aattcttcac
     6541 gtagtcggtc aacctgagcg gcacttaaac cggtataact gaacatgccg cgctgattaa
     6601 gcagataatc gaaattgcgt tctggcatct ctgtgcttaa taccttcacc aattcctgac
     6661 gcattgccag aatgcgagta cgcatctctt ctacttccgc cagccagctg gctttcaatg
     6721 cctcgtcatt cagcactgca gccaccacct gcgcaccaaa attcggcggg ctggagtagt
     6781 tgcggcgaac tgttgctttc aattgcccca gtacgcggcc agcggcttcg gcatcttcac
     6841 acagaacaga aagtccgccg acgcgctcgc cgtaaaggga gaaaattttc gagaacgaat
     6901 tgctcaccag agcgggtaat ccagcactgg caatggcgcg aatagcgtag gcatcctctt
     6961 ccataccggc actaaatcct tgataggcaa tatcgaggaa tgggataagc tcgcgggctt
     7021 tgagaatttc aatcaccgca tcccactgat cattagtgag atcggcaccc gttgggttgt
     7081 ggcaacatgg atgcagcaac acaatactgc gggcaggtaa tgttttcagc gtcgccaaca
     7141 ggtcattaaa gcgcacgctg ttagtcactt cgtcatacca ggggtaagta ctcacttcga
     7201 atccagcccc ggcgaatatt gctacgtggt tttcccaagt aggatcgctg acccagacgc
     7261 ctgattccgg gaagtagcgt ttcaggaaat ccgcgcccac tttcaatgcc cctgagccgc
     7321 caagggtttg aatggttgct acgcgctgtt gttgcagtac cggatggtcc gcaccaaaca
     7381 gcagcggcgc aatggcatgg cgatagctgt taagcccttc catcggtaaa taaagcgaag
     7441 cgccatgggg ctgcgcattc aggcgcgctt ccgcctccgc cacggctttc aattgtggaa
     7501 taattccgtc ttcgttgtag tacagaccga tacttaaatt cactttgtcg ctgcgagggt
     7561 cttctttaaa acgctccata agcgtaagaa tcgggtcgcc agcgtaggcg tcaacttttt
     7621 gaaacacgcg atggttctcc aggtttacgg gcaggtggtt aaaacacaat aaaccggaag
     7681 aaggcgaaga tcgagtggat gttcaggagc gaacggcaat tagcaacaga gtgagactaa
     7741 tgataaacgt acatccgcca gaggcacgac ctttataaga gcatgaaaga acatcaactt
     7801 attgaatttt taggatttta ttggccggat aaggcattca cgccgcatcc ggcacagaca
     7861 atcaaatatt acagaacgat taatccacgt atttcatcgc gacccttgaa gtcaggcgcg
     7921 taataagttc gtaagcgctt actttcgtca tttcagcgat acgttctacg ggcaaacctt
     7981 cgccccataa aatgaccgga tccccggctt tgtcctgcgc ctgtggacct aagtctacgc
     8041 agatcatatc catcgccacg cgcccgacaa tcggtacttc gcgaccgttc accagcactg
     8101 gcgtaccgga cggcgcggcg cgcggataac catcgccata gcccatcgcg actacgccaa
     8161 gacgggtatc acgttcgctt acccaggttc caccataacc aacaggctct ccggctttat
     8221 gctcacgcac ggcaatcagg ctggaggtta gtgacatcac tggctgacag ccaaaatcgg
     8281 caccggtgga gcgatcttcc agcggcgaga cgccataaag aatgatgccc gggcgcaccc
     8341 agtcaaaatg cgactgtggc cacagcagaa tgccacccga cgcggcaatg gaacgttgac
     8401 caggtttgcc ttcgcaaaag gtattaaaga tagcgagttg tttctcggtt gcgccacatt
     8461 ttggttcatc cgcgcgcgca aaatggctga cgatattcac cggctgacga acgtttttgc
     8521 actgggtcag gcgatgataa aacgcctcag cctgttccgg ccttacgccc agacggtgca
     8581 taccggtatc gagtttcatc cagacggtaa ccggctcgtc caagctagcc tcttccagcg
     8641 cagccagctg ttcttcgtta tgcacggcgg tatgaaaatg ttgcgcagaa atcgtcggca
     8701 gatctctggc atcaaaaaag ccttcgagta acagtacagg tttggtgatt ccccccgcac
     8761 gcagtcgcag agcttcttcg agacgggcta cgccaaaggc gtcagcatcg gggagcgttc
     8821 gcgcggtctc aagaagaccg tgaccataag cgttcgcttt caccaccgca accattttac
     8881 tggcaggtgc cagttcacga agacgttgca ggttgtgtcg cagagcgcgg cggttaatca
     8941 caacagttgc cgcttgcatt tgtgttcctt gataattgtt tgctttaatt acctaattca
     9001 taaaataatt attattcgtc gtcgtactgc ggccccgcat agttgtcgaa gcgcgaccat
     9061 tgaccgttaa aggtcaggcg taccgtcccg attgggccgt tacgttgttt accgataata
     9121 atttccgcga tgccttttaa atcactgttt tcgtgataca cctcatcacg atagataaac
     9181 atgatcaagt ccgcatcctg ctcgatagag ccagattcac gcaggtcgga gttgaccgga
     9241 cgtttgtcgg cacgttgttc cagagaacgg ttcaactggg acagcgccac caccggcacg
     9301 ttcagttctt tcgccagtgc tttcagcgag cgggagattt ctgcaatttc cagcgtacgg
     9361 ttatcggaaa gcgccggtac gcgcatcagt tgcaggtagt cgatcatgat aagcccgatg
     9421 ccgccgtgtt cacgggcaat acggcgtgcg cgggaacgca cttccgttgg cgtcaggccg
     9481 gaagagtcat cgatatagat attgcgtttt tcgagcaaaa tacccatggt gccggaaatg
     9541 cgcgcccagt cttcatcatc gagctgcccg gtacggattt tagtctggtc aacgcgcgac
     9601 agcgacgcca gagaacgcat catgatctgt tctgatggca tctccagcga gaagataagt
     9661 actggtttat cctgcaacat tgccgcgttt tcgacgaggt tcatcgcaaa tgttgtttta
     9721 cccatcgacg gacgcgcggc gacgatgatc aaatccgacg gctgcaagcc agcggttttt
     9781 ttgttgagat cgtcataacc ggtgtttacc ccggtaacgc catcgtgtgg ctgctgaaac
     9841 aactgctcaa tacgcgccac ggttgcgtcg agcacatcgg cgatgttctt cggcccttcg
     9901 tctttgtttg cacgactttc ggcaatttta aagacgcggg attcagcaag gtccagcaga
     9961 tcttcgctgg tacgcccctg cggatcaaaa ccggcttcag caatctcatt cgcaaccgag
    10021 atcatctcac ggacaacggc acgttcacgc acgatgtccg cataagcact gatgttcgcc
    10081 gcacttggcg tattttttga cagctctgcc agataagcaa aaccaccgac gctatcgagt
    10141 tgcccctggc gttccaacga ttccgcaagg gtaatcagat cgataggact accgctttcc
    10201 tgcaaacgcg ccatttcagt aaagatatga cggtgtgggc gggtgtaaaa atcgtctgct
    10261 accacacgct cggctacatc atcccagcgt tcgttatcca gcattaaacc gcccaacacc
    10321 gactgctccg cttcgatcga gtgcggaggc actttcagcc cggcaacttg tggatcgcgt
    10381 tcgcggggtt cagcctgctg tttgttgaag ggtttatttc ctgccatagt gaatggagtt
    10441 accgaaataa agaatgggtc gaaactttac catatgaagc agaccctgac gatacgttct
    10501 ggaggacaca tggcaacacg aattgaattt cacaagcacg gtggcccgga agtacttcaa
    10561 gccgtagagt tcactcctgc cgatccggcg gagaatgaaa tccaggtcga aaataaagcc
    10621 atcggcatca attttatcga cacgtatatc cgcagcggcc tttacccgcc gccatcgcta
    10681 cccagcggat taggtaccga agcggcaggc atcgtgagta aagtcggcag tggtgtaaag
    10741 catattaagg ctggcgatca tgtagtctat gcgcagtcgg cgttaggcgc ttacagctct
    10801 gtgcataaca ttaatgcgga taaagcggcg attctgcctg cggcaatttc ttttgagcaa
    10861 gctgcggcat ccttcctgaa aggcttaacg gtttattatc tgctgcgcaa aacctatgaa
    10921 attaaacccg atgaacagtt cctgttccac gcagcggctg gcggcattgg cttaattgcc
    10981 tgccagtggg caaaagccct gggcgcgaaa ctcatcggca ccgtaggaac cgcgcaaaaa
    11041 gcgcagagcg cgctaaaagc cggcgcgtgg caggttatta actatcgtga agagaatctg
    11101 gtcgagcgat tgaaagagat caccggcggt aagaaagtgc gcgtagtgta cgattccgtg
    11161 ggcagagaca cctgggaacg gtcgctggat tgcctgcaac gccgcggctt aatggtcagt
    11221 tttggcaact catcaggtgc ggttaccggt gtgaacttag gcattctcaa tcaaaaaggc
    11281 tcgttgtatg tgacacgccc ttccctgcaa ggctatatca ccacgcggga ggaattaacc
    11341 gaggccagta atgaactgtt ctctttgatt gccagcggtg tgattaaggt cgatgtcgcc
    11401 gagcagcaga aatatccgct gaaggatgcg cagcgtgcgc atgagattct ggaaagccgg
    11461 gcgacgcaag gttccagcct gctgattcca taaaagaaat agggcttcca cctgggaagc
    11521 cctttctttt tatagttcgg ctgtatgtag ggtacagcac gatgaatctg ttagaggcgc
    11581 aatagtgaca gatttgatta tcaattccta ttttgttcta aggataaaac cttaggttgt
    11641 gatcatccgc acaatccctt agtaacgcca gcggtcataa cgctgatatt tcggcatttt
    11701 tggtgcttta atcgccttaa taacccacac caccgcaatc gccagtagta accacggcag
    11761 caacttaatc atcaatgcca gcataccgcc gaggaacata atggccgtcg ccacaaccag
    11821 cgcggcgata atgcccagca acgaaacgcc ggtgaccatc agcatgacaa aaaagccaat
    11881 cacaaaaagt agttccagca tgatgctctc ccaaatatga aatctcttgc tggcattaca
    11941 agaatcatgc caaaaataat ctattgattt aacagcaaaa cgccccgcga cggtgcgcag
    12001 ggcgtggtga atttgactac tttttggtga aaagttaacg cttatccgcc accagtttga
    12061 gcgcgtgttc cagcacgtta atgtcagcac ccgctttatg ggcattttca cttaaataac
    12121 gccgccactg ccgcgcgcca ggaataccct ggaacaaacc cagcatatgc cggataatat
    12181 ggccgagata cgtcccctgg ctgagttcat gctcaatgta cggatacatg gcacgcacta
    12241 ctgccaccgg atcggcatcg gtatccgagg aaccaaagat ctcccggtct accgccgcca
    12301 gaatacccgg attctgatac gcctcgcgcc cgaccatcac gccatccata tgttgcaaat
    12361 gcgctttagc ttcttccagc gacttgatac caccgttaat cgacattgtc agatgcggga
    12421 agtcacgctt cagttgatac acacgcggat aatcgagcgg cgggatttca cggttttctt
    12481 tcgggcttaa ccccgaaagc caggctttac gtgcgtggat gatgaacatc tcacactcgc
    12541 ctttgccgga aacggtattg atgaaatcgc agagaaattc atagctgtcc tggtcgtcga
    12601 tgccaatacg cgttttcacc gtcaccggaa tcgacaccac atcgcgcatc gctttcacgc
    12661 agtcggcaac cagctgcgca ttacccatca gacacgcacc aaacatgccg ttctgcaccc
    12721 ggtcagacgg gcagccgaca ttcaggttga tctcatcata tccgcgcgct tctgccagct
    12781 ttgcacactg cgccagcgcc gccggatcgc taccgcccag ttgcaacgct accggatgtt
    12841 cttcttcact gtacgccaga taatcacctt taccgtgaat tatcgcccct gtggtcacca
    12901 tttcggtata aagcaacgta ttgcgggaaa gcagacgcaa gaaatagcgg caatgtctgt
    12961 ccgtccagtc gagcattggt gcaacactaa accgaccact ccagtgaacg tcagtttttt
    13021 caggcattgc gctggtttgg ttgatttttt gcatttcaga attaccgtgc attttcaaat
    13081 gtagagatta ttttatcgat atatcatggg gttatgttat tcagcttcac tgttcaggag
    13141 gctcaatagc ggggtactat accataacaa caggaagcgc ctgtctcatt gcaaaagaaa
    13201 attgagatcc tctcaaggca tgaagctctc acgaagtgat ggaaataatc ttattagccg
    13261 ttaactttgt taaggccaat gataaacaat ccaggttcga cgataaataa aaaatcacac
    13321 attaaactct ggtgatatat ctccctgcta atagcattga tagagaaaaa agaacccaat
    13381 aaatattggg tccttttata taatgccttc catactatcg aagaacttca catattattt
    13441 ctccgattta accccgaaca agtcataaat taatttagaa gtgtctgtaa gtattttaat
    13501 ctcttccttt gaggttgggt caaacgactt cgcgaagcta attaattgtg gtgcagcatc
    13561 tcgcattttg cttaaaatat caggatcgag cgttccttca ttcaccagat gtgacatctt
    13621 atccagatag ccatcaaaat tcatttctcc cccatctgcc agacgcttca tctctcccag
    13681 gtacttcttc atatcacgtt gatataattt ttcaaactga gctttcaggt agctctcatt
    13741 attttcatta atatacactg tttctgataa atctgcgtag gcactagaat atgaaagtga
    13801 aattaacagc gaagcaaata attttaagct gttgtcatca tgttcctggc aggcattaac
    13861 aaacgttaaa aaaccagaac ctattgcttg aaaatgtata ttcgctaaag gttcattatc
    13921 cttagattcc tcataaaata gctgaaccgt ggagggaagg ccattcttag tttctacatc
    13981 aaaagtacat tgttcaatgg gcattgcatc tttattatcc ccaggattac atatactatt
    14041 aatcgcgaaa tgaaaaatag cccggtcaac aactgatgca ataaagtcac gatcgtttaa
    14101 atcctcatca gtgcaatcgt cagtatactt attgacccac attttgagat catttatgtg
    14161 ctcaattaaa aacacatttt tttacgtgca tgccataaat tatgcgtgat tcgttgaata
    14221 aacaaatcta tttttgcaat aattatatcc cgatgacgca gcgctgcata atcaatagat
    14281 ttaccatata tattatttaa tttacctttg attgaacctc tattatccaa tttatctttg
    14341 gttgcatctt tattttcgaa ttgatttgaa ttgagtgttt tattaaagta tccagttgca
    14401 ccgggtatta tcttcaacat gattcatcta ccttttttgt cgtaccgtgt cctccttata
    14461 attcttttgc catctggtca ctatcaaaaa tcgctcataa tttctgaact acaggtatgc
    14521 gtttaaaccc agttaaataa atagcgagtt aacttgaagc aatttattta aaaatgaatt
    14581 attaattatt atcgtgttga taatggtacg ataaggcaac acaacacctt acctacaaaa
    14641 cagaaaatca acattacggc aacaataagg gttctcggtg tttttcctgg aacatggtaa
    14701 agtaaggaca ttcttaaccc ccactttgag gtgcccgatg gaaaagacca caacgcagga
    14761 gttattagcg caggctgaaa aaatctgcgc gcagcgtaat gtgcgcctga ccccacagcg
    14821 cctggaagtg ttgcgcctga tgagtctgca ggatggcgct atcagcgctt atgatctgct
    14881 tgatttactg cgcgaagctg aaccgcaagc caagccgcca acggtttatc gcgcgctgga
    14941 ttttctgctt gagcaaggtt ttgtgcataa ggtggaatcc accaacagtt atgtgctctg
    15001 tcatctgttc gaccagccca cgcatacgtc agccatgttt atttgcgatc gctgcggcgc
    15061 ggtgaaagaa gagtgtgcag aaggcgtgga agacattatg catacgctgg cggcaaaaat
    15121 ggggtttgcc ctgcggcata atgtgattga agcacatggg ctctgtgcgg catgcgtgga
    15181 agtggaagcg tgtcgtcatc ctgaacagtg ccagcatgat cactctgtgc aggtgaaaaa
    15241 gaaaccgcgt taagagggcg tacatccttg tacacgtcgg gcaggaggga ttaattacca
    15301 gcgatattca ttgcgggttt cccaatccac gacctctttt tctgcctgat ctttctgata
    15361 accataacgt tcctggattt taccgaccag ttgatcacgt ttaccttcaa tgatcgtcat
    15421 atcatcatcg gtcagtttgc cccattgctc tttcacttta cctttaaact gtttccagtt
    15481 accgccggct tcatctttat tcataatcaa gacctcatcg ttaggttgtg aatgagagta
    15541 cgttcacttt tcttctgaac gtgagattaa gtgtagtcag caatttggct taggataatt
    15601 tattcagaat ttttaaccgt cacgttgcgg caaaccaggt accgttgcgc cagtgacgcc
    15661 gccagatagc cgccagagaa agcccgcgca acgccagaaa gacggttaat gccagccaca
    15721 aaccatgatt acccagccac ggcagcgtaa ggagcgtcag cgcaaaacct gcggcggcca
    15781 ccgccatact gttacgcatt tcggcggcac gcgttgcgcc tataaacatg ccgtccagca
    15841 gataacacca gacgccaacc aacggcaaaa tcacctgcca gataagatag cggtcagcca
    15901 gctgctgaat ctgggttaac gacgtcagca acgcaatgat gtgttcccct gccagcaaat
    15961 aaaccaccga aaatagtaac gctacaatcc ccgactggcg gcacgctgcc cgccatacat
    16021 ccagcaactg gctaccgtcg cgcgcaccgt atgcctgacc ggagtgcgct tcaaccgcgt
    16081 aggcaaaacc atcaagcgca taggcggtaa aggtgagtag cgtcatcaga accgcgttaa
    16141 cagcgataat gtcactcccc agtcgcgcac caagtacggt gatcgcgccg aaacagagtt
    16201 gcaacaacag cgaacgcagc atgatatcgc ggttaagcgc cagcaagcga cggaagtttc
    16261 ctcgccaggc agttttcagc atttcgccgg aaattccgcg tagtttgagg attttacgca
    16321 ccattagcag accaatcagc aatgttgcat attccgcaat aaccgtcgcc agcgccgcgc
    16381 cctgcacgtt catatgcagc cccatcacca gccagacatc cagcacaatg ttgaggatat
    16441 tgccgaccac taacaaaatt actggcgcac gggcatattg cacgccgagt aaccaaccga
    16501 gtaataccag attcgccagc gacgccggag cgcttaacca gcggatttca agaaagcgcc
    16561 gcgcctgttc caggaccgct tcactaccgc caacaatatg cagcgccaga tcgataatcg
    16621 gcgtacgcaa cagcgcaatt aacgccccgg cccccaacgc caacagcaac ggttgcacca
    16681 gcgcacgggc taatgcctga ggatttttgg caccataagc ctgcgcagtc agcccggtgg
    16741 tgctcatgcg taaaaacagc aacagcataa agagaaagct ggtcgccgtt gcaccaaccg
    16801 ccacgccgcc caaataaacc ggactatcaa gatgaccaat taccgccgta tcgaccagtc
    16861 ccagcaacgg aacggtgata ttggagaaaa tcatgggtaa ggcgagatgc cagagtgctt
    16921 tatcagatga agtgaggaat gccatgcaga caagcctgat gaagagagat gaaaaacaaa
    16981 ccgcgacgcc aagcggcatc gcggtctcag agatatgtta cagccagtcg ccgttgcgaa
    17041 taaccccaac cgccagccct tcaatggtga agctctgctg acgaaggtca acgacaattg
    17101 gtttaaactc gctattttct ggcaacagtt cgactttatt gccctgtttt ttcaggcgct
    17161 taacggtaac ttcgtcatca atacgtgcga caacgacctg accgttacgt acatcctgag
    17221 ttttatgcac tgccagcaag tcgccatcca taatgccgat atctttcatc gacatcccgc
    17281 tgacgcgcag caggaaatca gcattcggct tgaacaagga aggatcgacc tgataatgac
    17341 cttcaatatg ctgttgcgcc agaagcggtt caccggcagc cacacgacct accagcggca
    17401 acccttcttc ctcttcctgc aacagacgaa tcccgcgtga tgcgccggaa acaatttcaa
    17461 taacgccttt gcgtgccagc gccttcagat gttcttcagc cgcgtttggg gaacggaacc
    17521 ccaaacgctg cgcgatttcc gcacgcgtcg gcggcatacc tgtctggctg atgtgatcac
    17581 ggatgagatc aaacacctct tgttgcctgg ccgttaacgc tttcattccg ccccctgggt
    17641 gtatatacag ttatactgtg agtatataca gcaaaaggcg attttggaac cataaactgc
    17701 acaataaacc agagatttat cgaaatctgg aagggttatc caaaatgcga ccataacagg
    17761 atgcaccagg taatcacggc gacgataatg gcaatcagca ccgcggcgga tcccatatct
    17821 tttgcgcgtc cggaaagctc atggtattca gagccaattc ggtcaaccac tgcttcgatg
    17881 gcgctattga ggatttccac aatcatcacc agcatcacgg agctgataag cagcacgcgg
    17941 gtaattgcgt ccacatccag ccagcaggcg atgaccaccg ccaacaatac cgctacgcct
    18001 tcctgacgga atgccgcttc gttgatccat gcagcgcgta aacctttcca ggaatagcca
    18061 gcagctttga taattcgggt gaatccagtt gtattattgg ccattgaaag aaccttttta
    18121 cattatgagc gtcaatatca gtgtaccgtt aaatccgatg attcgggttt gcgtaacgcc
    18181 gcgcgaaaca tgagcggata ccacagaatt tcccatgact ttctgctatc cttgccgcgc
    18241 atttgcatta ttaaccagag gctttacatc gtttatgtcc ggctggccac gaatttacta
    18301 caaattactg aatttaccat taagcatcct ggtaaaaagc aagtctattc cggcagatcc
    18361 tgccccggaa ctggggctgg atacctctcg tccaattatg tacgttttac cgtacaactc
    18421 gaaagcagat ttgctgacgt tgcgcgccca gtgtctggca catgatttgc ctgacccgtt
    18481 agagccgctg gaaatcgacg gcacgctact gccgcgctat gtgttcattc acggcgggcc
    18541 gcgtgtattc acctattaca cgccgaaaga agagtctatt aagcttttcc acgactatct
    18601 cgatttgcac cgtagcaacc caaatctgga tgtgcagatg gtgccagtat cggtgatgtt
    18661 tggtcgcgcg ccggggcgtg aaaaaggcga agtgaacccg ccgctgcgta tgcttaacgg
    18721 cgtacagaaa tttttcgctg tactgtggct cggtcgcgac agttttgtgc gtttctcgcc
    18781 gtcagtgtcg ctgcgccgta tggcggatga acacggcacg gataaaacta tcgctcagaa
    18841 actggcgcgc gtggcgcgta tgcactttgc ccgtcaacgt ctggctgccg taggcccacg
    18901 tctgcctgct cgtcaggatc tgtttaataa gctgctcgcc tcccgcgcca ttgccaaagc
    18961 ggtagaagat gaagcgcgca gcaaaaaaat ctcccatgaa aaagcgcagc agaacgcgat
    19021 tgcgctgatg gaagagattg cggcgaattt ctcttacgag atgatccgcc tgactgaccg
    19081 cattctgggc ttcacctgga accgatttta tcagggcatc aacgtccata acgccgagcg
    19141 cgttcgccag ctggcccacg acggtcatga gctggtgtat gtgccttgcc accgcagtca
    19201 catggactac ctgctacttt cttacgtgct gtaccaccag ggactggtgc cgccgcatat
    19261 cgccgccggg atcaacctga acttctggcc ggccgggccg attttccgcc gtctgggggc
    19321 gttctttatt cgccgtactt ttaaaggcaa taaactttat tccaccgttt tccgtgagta
    19381 tctcggcgaa ctgttcagcc gtggttattc cgtcgagtac ttcgtggaag gcggtcgttc
    19441 ccgcacgggg cgtttgctgg atccgaaaac cggtacgctg tcgatgacca ttcaggcgat
    19501 gctgcgtggc ggcacgcgtc cgattacgct gattccgatc tatatcggtt atgagcacgt
    19561 catggaagtg ggtacttacg ccaaagaact gcgcggcgcg acgaaagaga aagagagcct
    19621 gccgcagatg ctgcgcggtt taagcaagct gcgtaatctc ggtcagggtt acgtcaactt
    19681 cggtgaacca atgccgttga tgacctacct taaccagcac gtaccagact ggcgtgaatc
    19741 tatcgatccc atcgaagcgg tgcgtccggc ctggttaacg ccgacggtca ataatattgc
    19801 tgccgatctg atggtacgca ttaacaacgc aggcgcggca aacgccatga acctgtgctg
    19861 tactgcgcta ctggcatcac gtcagcgctc actcacccgc gagcagttaa ccgagcaact
    19921 caactgctac ctggatctga tgcgcaacgt accttactcc acggactcta ccgttccttc
    19981 agccagcgcc agcgagctta tcgatcacgc gctgcaaatg aacaagtttg aagtcgagaa
    20041 agacactatc ggcgacatca tcattctgcc acgcgagcaa gcggtgctga tgacctacta
    20101 tcgcaacaac attgcgcata tgttggtgct gccttcgctg atggcggcaa ttgtcaccca
    20161 gcatcgccac atctcccgcg acgtattgat ggagcacgtc aatgtgcttt acccaatgct
    20221 gaaagcggag ctgttcctgc gctgggatcg cgacgagttg ccggacgtta ttgatgcgct
    20281 ggcaaatgag atgcaacgtc aggggctgat taccctgcaa gatgatgagt tgcatatcaa
    20341 cccggcgcat tctcgcaccc tacagctgct ggccgcaggc gcgcgcgaaa cgctgcaacg
    20401 ttatgccatc accttctggt tgttgagtgc caacccgtcg atcaaccgcg gtacgctgga
    20461 gaaagagagc cgcaccgttg cgcaacgtct ctccgtgctg cacggcatca acgcgccgga
    20521 gttcttcgac aaggcggtgt tcagttctct ggtgctgacg ctgcgtgatg aagggtatat
    20581 cagcgatagc ggcgatgccg aaccagcaga aacgatgaag gtttatcagt tgctggcgga
    20641 gttgattaca tcagacgtgc gtttgacgat tgagagtgcg acgcagggcg aagggtaatc
    20701 agagagaatt gccggatgcg gcgaaaacgc cttatccggc ctaccatgac ctgcaaattc
    20761 aataaattgc gattcaccgg gtaggcctga gaagcgcaac gcatcaggca acccagaaga
    20821 aagccggatg atcatccggc ttttttacat catcagaaat gccagtaact cattgccagt
    20881 cctaaaaaca gtaccagacc aacatagtta ttattcataa atgctttaaa gcaggcttca
    20941 cgctcgcggt tggcaatcag tttttgttga taaacaaaca gcgcgccagc caccacaatt
    21001 gaccagtaat atccccagcc taagccattt aactcaccga tgatcgccat cagtgccagt
    21061 acgccaatct gcaaaatacc aataatcaat ttatcgtatt ggccgaacag gattgccgtg
    21121 gatttaatgc caatcttcac atcatcatcg cggtcaacca tcgcatactg cgtgtcgtaa
    21181 gccaccgccc agagaatatt ggcgaggaac attaaccagc aactcaatgg caccgactca
    21241 ctcacagcgg caaaagccat tggaatcgac cagccaaacg ccgcgcccag caccacttgc
    21301 gggagatggg tataccgctt cataaacggg tacacccacg ccaatgccag agcggcaatc
    21361 gacaacagaa tggtcatcgt attcagcgtc agaaccagta aaaacgaaat cagtaccagc
    21421 actacaaaca gcgcgcgcgc ctctttctct gttaccgcgc cgctgggaag tggtcggttc
    21481 gccgtgcgct taacatgacc atcaaacttg cgatcagcat aatcattcac cacgcaacca
    21541 gcagcgcgca tcagccagac acccgcgaca aacaccgcca tgatccagag ctgaggaacg
    21601 cccggtgtcg ccacccacaa cgcccataat gttggccaga gcagcagtaa cgcgccaatt
    21661 ggcttatccg tacgcattaa gcgatgaaac gccagcagct tattctgcgt cagactccac
    21721 tccatatttt tttcctctta gtacaacggt gacgccggta aaaacagttc tgttagcaac
    21781 agcggtttac cgcttaatcg caggcgggaa cgtcgccccc acagtccggc atcacggcct
    21841 atctcgataa aatcccgggt tagtgtcgat gatgtgaaca gatagcgtcc taacggcgtt
    21901 ttacccaatt tttgtaacgc tagttccggc ccgcttaatg ttgatacagg aacgacggta
    21961 cgaccggcaa gccacggttc accatcggca cataacaaaa tttcacgtaa ccagtaacga
    22021 gactctttcg gcagcagcgg cagttcttcg gggatttcat tctgctcgac aaacccctcg
    22081 cggatcatcg tcacactcac cgtttttccc tgctgttcaa aacgttttgt catggaatcc
    22141 tccagcaaca gccagtcgag cagttgcgga tccagggcag ggatctcttt aaaatagcgc
    22201 agcgcacgca gttgcgttaa cgcggggtgt gacatgccga actctccgtt acataacgta
    22261 aaggcattgt atcgcagaaa agtgtttacg ggagatgtaa agcaaattaa gtgatctaat
    22321 gcgcaacaaa aacgcaacaa tcgcatgaag tggtaaaacg ctgttgaacc ttacgctttg
    22381 ccgatgcccg tgctggcgag ggcaaccata ggggcatcac cgcccatgat gctaggaacg
    22441 gtgatgtttt cctttactta aataccttta ttctttatac gagtagctcg ccaactcgca
    22501 gtaaaaattg ggaaaagcgc atatttacca tgagatttgg agctgaaaag tgtttcctga
    22561 gtaagattaa taggcaacat cttatgtctg gatttataga tacacgtgga attccaattg
    22621 gtatcttttg taggattcaa catatcatgt aagcgctggg agcttaccac cataacaggc
    22681 ccatcatcga cagaggagaa caggataaat atattatcgt ttcgtgttaa tttattagtg
    22741 ttaaaggttc ttaacttttc ttcagtttct ataaatatag gtgaaacatc atcactacga
    22801 atccactctt tataaagata ccaggcattt gctttgatca tatcatccgt tgatcgtaag
    22861 gcctgatcga taagttggat aaaaaaattg ttatttttat caaccagccc tttattcata
    22921 ttaaatctgt taataaaatg gatcatctgt tgggtatcga aattcacctt actgaatcga
    22981 tgttgacgca taaaattatc gaacttgtca tcttccccag aatgtatata actacgtata
    23041 attggatctt ttatataagg acaatggcta aatgttttct tcatattctc aataaagttt
    23101 ttattaacaa tattgagaat ttttaattgc atcaccagtt ttactgccag aacggatttc
    23161 aatatcattg cattatctgg caatgtatca atcattttta taatgttatt gatataacca
    23221 tcgagatctt cattcccaaa tatatctgaa taatcaatag ttaattccaa ttgttctgta
    23281 atgcaatctt aaaaattccc tgtcgacatt cgcacatgat caaacataac agctgtcaaa
    23341 ttcgatttaa taaatgatat ttctttggca gacagaaact ctgctctggt gaagtgcgaa
    23401 gcgtaaagat aacaattatc gaagtttgaa tttctaatct gacattcgcg aaaattacta
    23461 tgaccaagtt gactctgcga gaagtcaact gtatcaagaa tattttggct aaaatcaaca
    23521 cattcgccac tcgttttgag aaaaatcaca ttgtgtaaac gtcgacattt aaatatgaca
    23581 ttatcaactg ttgccttagt aaaattgcaa ttctcaattt ctttacattc gaaagttaca
    23641 tgttgtagat ttgatgataa aaaagacacc ttagaaataa cgtcaccttt aaaatgacaa
    23701 tttgttaaat caaaatcaga caggtcgata tacccctgcg catctttatc catcagaaaa
    23761 ttacaagcgc attctatttt ttcatttctg tagaatttta cacttatagt atcattacta
    23821 attgagactt cacgtaacat atttattgca tgattttttt cgttccgttg ttttatattt
    23881 aaaaatatat cactgatata aataaagtaa tctctgttta aacctgtcac cccattgaaa
    23941 ttacgtagta aatcaagatc tactatatta tttccttgca ccacatcatg tttcatcaaa
    24001 gagttattgc cgccagaatt tgtgatatga cctatcataa aacctctgta atacagaaat
    24061 aaaataacct ggaaaagtag cctgagcgtc cagacactac cggcgaaagg aaaagaatct
    24121 ctgataatgc attgaaataa tggacattct tagtgaagaa gaagtgaagt actataaaaa
    24181 atcgctcata tcccaggaag gaagcataaa aaaaggtgcg ccaggagacg caccagttgt
    24241 tgcaaaatca gcactgtggg gcgtaattac cccttgcctt ttacactgct gataaaggtg
    24301 gaacgggcag atgtcgatcc caggcgttca gcttcatcaa gcagttttaa cgccttatca
    24361 acatcacctt tcgcgacagc gtttttgatc gcggtattaa aataactttc cgtgtcgttg
    24421 agcatcggct cgcttttctt caccggcacc ggagcaggtg cagccacagc tggtgccgcc
    24481 gtgttgccta ccgtaaccgg agctggagcg gaagaaccaa atagcggtcc taccaacacg
    24541 ctggagctgg agttcgtttt gactttcagt ttcagtaagc catcgctggt atgacgagca
    24601 accggatcgg gaatatccgg gatcgagtta ccgacgcctt tggcataggc tttagccggg
    24661 tcgagcagtt gggtcgtctg ctggagatct ttttccgtgg taaagaccag aacataaagt
    24721 ttttgctgcc ccaacgccgg tgtcaggcgc ataacgcctt ccagccgatc tgcactcatc
    24781 acgcctagtt cctggtaggt gaaataactg ctggggaaga aggctgatgg ggtcatgttc
    24841 tgatcaagaa tcagcacgtt cggcgcaaaa acgctggttt gtttgttcac ttcgctggtc
    24901 agcgtcaggg tcagttcgcc aatgtttgcc gggacgctgt acgcagcaac cggaccactg
    24961 atgccgggaa cgttcagttg ttggccgccg gtcgccagtt gggtggtctg ggttttagat
    25021 tgatcgaccg gtgtccaggt gagttgttgc agcgcggcag atggaatggc tggcgcgtcg
    25081 ctggtgtttt gcggtacgta gttaacatcg gcaaggctaa ttccaggcgc gcttgccagt
    25141 aaccctgctg ataaacagag ggcgatgaga cttttattca ttttcattgt tttcacctca
    25201 aaatctggag ctcagcggta gccaggcaat agcgcgctaa acccgataat cagaggggct
    25261 ttcgcccctt tcagataatg acaacctgtt tttatgccgg atgcggcgta aacgccttat
    25321 ccggcccagg ttttgctatt accaccagat ttccatctgg gcaccgaagg tccactcgtc
    25381 gctgtcgcca cgaccgaagc tgccgccgtt gaaatcagca ggaacggctt tgccgtagtt
    25441 cgggttaacc ttgctatcgc cgttgtagtc gtaaccccat ttctcatccc acttggcgta
    25501 ggttgcgaag acacgaatag ccgggcgtga ccagatgctg tcgccagcct gccattgttg
    25561 tgcgagggta attttgtact gattgttctt gtcgccggtg cgctgggatt cgacgttgtc
    25621 gtagccgatt tccatcacgg tgctcatgat tggcgtccac ttgtacatcg ggcgaatacc
    25681 aacggtccac cacttggtgc cgttgtcgtt atcccagttg atatcctggt acatacccac
    25741 gtacatcatg tcccagttgt cgcccatgga gatcgcaccg tggtcgagga tacgcagcat
    25801 gtgaccgttg ttgttgatat tgtaggcaaa tttttcgtta tcaaacgcga cgccagaacc
    25861 ctgcgacaga cctttaccct gcgaggtcat cgagtcagta gcgtactgaa caacaaactt
    25921 gttaaagccc ttcaggacac tctgagtatg ttcagcagtg aataaccagc cgtctttcga
    25981 tgcgccatca accagacgat agttatcacg caggttggca cgaccgtagt cgacacccag
    26041 ttctaatgtg ccgcccgggt tgatttccat ctgcgctaaa cgcacatcga aaacgtcgtt
    26101 cgcggtttcg ttggtatagt cataaatatt gttgctggca aaagaggaag aaccaccagc
    26161 ttcagaggag cgggttgctg ccagagagag tttaccgaag ccaacatcga tgttttccag
    26221 accggcacca ggaccagaaa tatcccagta gtagaagtcg atcatatgaa cgtcatgacg
    26281 ttggtagaag cgcttacctg cccagatggt ggaacctggc agccattcga tcaggttttt
    26341 tccctgcacg tttgcttcac ggaaggccgg atcggtagct tcccagtcat tctgttgcgc
    26401 gacggaatag gccacgttag tgtcgaaata gaagctctta tcgccctctt tccacacttc
    26461 ctgacccaat tttaattcag cataagtttc acattcgttg ccaagacggt atttactttg
    26521 agcaccggta gtctggaaac actgttgttc accgccgcta cctgtccagc caataccgga
    26581 acgtgcatag ccgtggaaat caacagccat tgcctgagca gacattacgc ccgctgcgac
    26641 ggcaaccgcc agaggaagtt tgcgcagagt aatcatcatt ctatctcctg agtcattgct
    26701 tttctttttt cacatcacct gtgacaggct ttgtgtgttt tgtggggtgc ttaaacgccc
    26761 ggctccttat gcagtcgacg acatgcagtg ccatcctcac ggaacagatg gcaacgctct
    26821 ggcggcaggc cgatagcgaa tgtggcacct tcttctacca acaccacgtc gttctggcgg
    26881 tacaccaggt tttgacgaat ggaagggatc tggatataga tttgggtttc gttgccgagt
    26941 tgctcgacga cctgaacttc accctcaagg atgacgtcag cgatatcact cggcagtaga
    27001 tgttccgggc gaatacccag cgacatattg gctccaacct ggacatcacg gctttcaaca
    27061 ggcagccaga cttgttgacg atttggcatc ggcagctcca cctgcacttg atcgattgcg
    27121 gtggcggtca cttttaccgg caggaagttc atctttggcg aaccgataaa tccggcgaca
    27181 aaacggtctg ccggatagtg gtacagttcc agcggtttcc caacctgtgc cacgcgaccg
    27241 gcgtccagca ccacgatttt gtcggccagc gtcatcgctt cgacctgatc gtgggtgacg
    27301 taaatcattg tgcggcccag gcgtttatgc agacgggaga tttcgatacg catttgcaca
    27361 cgcagtgcag catcgaggtt ggagagcggt tcatcgagca aaaatacgct tggctcggcc
    27421 accagcgtac ggccaatcgc cacgcgctga cgctgaccac cggagagcgc tttcggtttg
    27481 cgatccagca aatgcgccag ttgcagcact tccgccacct ggttgacgcg ttggttaatc
    27541 acctcttttt ttgcgccagc cagtttcagg ccaaatgaca tgttttctgc tactgacagg
    27601 tggggataga gcgcgtaaga ctgaaacacc ataccaacgc cgcgttctgc tggcggagtg
    27661 tcattcatcc gtttctcacc gatgaacagg tcgccgctgg tgatcgtctc aagcccggca
    27721 atcatgcgca gtaaagtcga tttaccgcag ccagacggtc cgacaaacac cacgaattca
    27781 ccttcatgga tatcgagatt gatatctttc gataccacga cctcgcccca ggctttcgtt
    27841 acattttgca gctgtacgct cgccatgccc ttctcccttt gtaacaacct gtcatcgaca
    27901 gcaacattca tgatgggctg actatgcgtc atcaggagat ggcttaaatc ctccaccccc
    27961 tggctttttt atgggggagg aggcgggagg atgagaacgc ggcttctgtg aactaaaccg
    28021 aggtcatgta aggaatttcg tgatgttgct tgcaaaaatc gtggcgattt tatgtgcgca
    28081 tccccacatt accgccaatt ctgtaacaga gatcacacaa agcaacggtg gggcgtaggg
    28141 gcaaggagga tggaaagagg ttgccgtata aagaaactag agtccgttta ggtgttttca
    28201 cgagcacttc accaacaagg accatagatt atgaaaataa aaacaggtgc acgcatcctc
    28261 gcattatccg cattaacgac gatgatgttt tccgcctcgg ctctcgccaa aatcgaagaa
    28321 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
    28381 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
    28441 gagaaattcc ctcaggttgc ggcaactggc gatggccctg acattatctt ctgggcgcac
    28501 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
    28561 ttccaggaca agctgtatcc gtttacatgg gatgccgtac gttacaacgg caagctgatt
    28621 gcttacccaa tcgctgttga agcgttatcg ctgatttata acaaagacct gctgccgaac
    28681 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
    28741 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
    28801 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
    28861 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
    28921 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
    28981 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
    29041 acggtactgc cgaccttcaa gggtcaacca tctaaaccgt tcgttggcgt gctgagcacc
    29101 ggtattaacg ccgccagtcc gaacaaagag ctggcgaaag agttcctcga aaactatctg
    29161 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
    29221 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccaccat ggaaaacgcc
    29281 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtacgt
    29341 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
    29401 cagactcgta tcaccaagta atgctgtgaa atgccggatg cggcgtgaac gccttgtccg
    29461 gcctacaaaa ctgaaacgca tgtaggcctg ataagacgca tcagtgtcgc atcaggcagt
    29521 tgttgtcgga taaggcgtga aagccttatc cgtcctggaa tgaggaagaa ccccatggat
    29581 gtcattaaaa agaaacattg gtggcaaagc gacgcgctga aatggtcagt gctaggtctg
    29641 ctcggcctgc tggtgggtta ccttgttgtt ttaatgtacg cacaagggga atacctgttc
    29701 gccattacca cgctgatatt gagttcagcg gggctgtata ttttcgccaa tcgtaaagcc
    29761 tacgcctggc gctatgttta cccgggaatg gctggaatgg gattattcgt cctcttccct
    29821 ctggtctgca ccatcgccat tgccttcacc aactacagca gcactaacca gctgactttt
    29881 gaacgtgcgc aggaagtgtt gttagatcgc tcctggcaag caggcaaaac ctataacttt
    29941 ggtctttacc cggcgggcga tgagtggcaa ctggcgctca gcgacggcga aaccggcaaa
    30001 aattacctct ccgacgcgtt taaatttggc ggcgagcaaa aactgcaact gaaagaaacg
    30061 accgcccagc ccgaaggcga acgcgcgaat ctgcgcgtga ttacccagaa tcgtcaggcg
    30121 ctgagtgaca ttaccgccat tctgccggat ggcaacaaag tgatgatgag ctccctgcgc
    30181 cagttttctg gcacgcagcc gctctacaca ctcgacggtg acggcacgtt gacgaataat
    30241 cagagtggcg tgaaatatcg tccgaataac caaattggct tttaccagtc cattaccgcc
    30301 gacggcaact ggggggatga aaagctaagc cccggttaca ccgtgaccac cggctggaaa
    30361 aactttaccc gcgtctttac cgacgaaggc attcagaaac cgttcctcgc cattttcgtc
    30421 tggaccgtgg tgttctcgct gatcactgtc tttttaacgg tggcggtcgg catggttctg
    30481 gcatgtctgg tgcagtggga agcgttgcgc ggcaaagcgg tctatcgcgt cctgctgatt
    30541 ctgccctacg cggtgccatc gttcatttca atcttgattt tcaaaggatt gtttaaccag
    30601 agcttcggtg aaatcaacat gatgttgagc gcgctgtttg gcgtgaagcc cgcctggttc
    30661 agcgatccga ccaccgctcg cacgatgctg attatcgtca atacctggct gggttatccg
    30721 tacatgatga tcctctgcat gggcttgctg aaagcgattc cggacgattt gtatgaagcc
    30781 tcagcaatgg atggcgcagg tccgttccag aacttcttta agattacgct gccgctgctg
    30841 attaaaccgc tgacgccgct gatgatcgcc agcttcgcct ttaactttaa caacttcgtg
    30901 ctgattcagc tgttaaccaa cggcggcccg gatcgtcttg gcacgaccac gccagccggt
    30961 tataccgacc tgcttgttaa ctacacctac cgtatcgctt ttgaaggcgg cgggggtcag
    31021 gacttcggtc tggcggcagc aattgccacg ctgatcttcc tgctggtggg tgcgctggcg
    31081 atagtgaacc tgaaagccac gcgaatgaag tttgattaag ggagataaca aaaatggcaa
    31141 tggtccaacc gaaatcgcaa aaagcacgtt tgtttattac tcacctgcta ctgctacttt
    31201 ttatcgcggc gattatgttc ccgctgctga tggtcgtcgc tatctcgctg cgtcagggaa
    31261 acttcgcgac cggtagcctg atcccggagc aaatctcctg ggatcactgg aaactggcgt
    31321 taggttttag cgttgaacag gctgatggtc gcattacgcc accgccattc ccggtactgc
    31381 tgtggctgtg gaactcggta aaggtcgctg ggatttccgc gattggcatt gtggcgctct
    31441 ccaccacctg cgcctacgct ttcgcccgta tgcgctttcc aggcaaagcg acgctgctga
    31501 aaggaatgct gattttccag atgttcccgg cagtactttc actggtcgcg ttgtatgcgt
    31561 tgtttgatcg tctgggtgag tacattccat tcattggcct gaatactcac ggcggcgtaa
    31621 ttttcgcgta tctgggtggg attgcgctgc atgtctggac catcaaaggc tatttcgaaa
    31681 ccatcgacag ttcgctggaa gaagcggctg cgctggatgg tgcgacaccg tggcaggcct
    31741 tccgccttgt cctgttgccg ctgtcagtac cgattctggc ggtggtattc atcctgtcgt
    31801 ttatcgctgc cattactgaa gttccggtcg cgtcgctgtt actgcgtgac gtaaacagct
    31861 acaccctggc cgtggggatg cagcaatacc tcaacccgca aaactacctg tggggtgact
    31921 ttgccgccgc tgccgtgatg tctgcattac cgatcaccat cgtcttcttg ctggctcaac
    31981 gctggctggt caacggcctg acggcaggtg gtgtgaaagg ttaaagatgt tgttctgcca
    32041 atgttatgcc gctgcaccct caacttacgt tatcccaact tgtgactatt attcggcgct
    32101 ccacggagcg cctttttttc tttcgtctgc aatctgaatc gttcgccggt taatattccc
    32161 atcatagaac ttattatttt tacgttattt gttttcccac ttacgataat tctctttcgt
    32221 gctctgagtc acggcaatag tattgtttca tcaatttttg ataattatca caattaagat
    32281 cacagaaaag acattacgta aacgcattgt aaaaaatgat aattgcctta actgcctgac
    32341 aattccaaca tcaatgcact gataaaagat cagaatggtc taaggcaggt ctgaatgaat
    32401 acccagtata attccagtta tatattttcg attaccttag tcgctacatt aggtggttta
    32461 ttatttggct acgacaccgc cgttatttcc ggtactgttg agtcactcaa taccgtcttt
    32521 gttgctccac aaaacttaag tgaatccgct gccaactccc tgttaggatt ttgcgtagcc
    32581 agcgctctga ttggttgcat catcggcggt gccctcggtg gttattgcag taaccgcttc
    32641 ggccgtcgtg attcacttaa gatttctgct gtcctgtttt ttatttctgg tgtaggttct
    32701 gcctggccag aacttggttt tacctctata aacccggaca acacagtacc tatttatctg
    32761 gcaggttatg tcccggaatt tgttatttat cgtattattg gcggtattgg cgttggttta
    32821 gcctcaatgc tttcaccaat gtatattgcg gaactggctc cagctcatat tcgcggaaaa
    32881 ctggtttcat ttaaccagtt tgcgattatt ttcgggcaac ttttagttta ctgcgtaaac
    32941 tattttattg cccgttccgg tgatgccagc tggctgaata ctgacggcag gcgttatatg
    33001 tttgcctcgg aatgtatccc tgcactgctg ttcttaatgc tgctgtatac cgtgccagaa
    33061 agtcctcgct ggctgatgtc acgcggcaag caagaacaga cggaaagtat cctgcgcaaa
    33121 attatgggca acacgcttgc aactcaggca gtacaggaaa ttaaacactc cctggatcat
    33181 ggccgcaaaa ccggtggtcg tctgctgatg tttggcgtgg gcgtgattgt aatcggcgtt
    33241 atgctctcca tcttccagca atttgtcggc atcaatgtgg tgctgtacta cgcgccggaa
    33301 gtgttcaaaa cgctgggggc cagcacggat atcgcgctgt tgcagaccat tattgtcgga
    33361 gttatcaacc tcacctttac cgtactggca attatgactg tggataaatt tggtcgtaag
    33421 ccactgcaaa ttatcggcgc actcggaatg gcaatcggta tgtttagcct cggtaccgcg
    33481 ttttacactc aggcatcggg tattgtggcg ctactgtcga tgctgttcta tgttgccgcc
    33541 tttgccatgt cctggggtcc ggtatgctgg gtactgctgt cggaaatctt cccgaatgct
    33601 attcgtggta aagcgctggc aatcgcggtg gcggcccagt ggctggcgaa ctacttcgtc
    33661 tcctggacct tcccgatgat ggacaaaaac tcctggctgg tggcccattt ccacaacggt
    33721 ttctcctact ggatttacgg ttgtatgggc gttctggcag cactgtttat gtggaaattt
    33781 gtcccggaaa ccaaaggtaa aacccttgag gagctggaag cgctctggga accggaaacg
    33841 aagaaaacac aacaaactgc tacgctgtaa tcttcctgtc cagcacgccg cgccatttcg
    33901 gcgtgctgtc tttttactcc cgcttcagcc gtttcgaatt acacagccac agggtgatca
    33961 ccagtaacag gatcgcagcc gagtagatca acacatccag tggcgattta tgatcgacga
    34021 tgatcaagcg cacaatggcg gtgatcccaa tatagacaaa gtaacgtagc gggaagtgaa
    34081 aaccggactg aaagtacttc acaatcagcg cgataaattc gaaatagaga aagtaaacca
    34141 ccagtccttc taccagctca tatttgctgg tttgttctgg cgcgaacagc acatcagcca
    34201 gatgcaccgt ttctttgccg aggaagacaa ccaaaatcag gccgaggcac agcaggccaa
    34261 gattgagtac ggtctgcaaa atggtggaga taaactccac gcgcggacga gagagtgacg
    34321 tcatagcagt acctccttca cagtggcgag cttcctgtat aacgcaaaaa tgtgacggag
    34381 atctatattt tttgtgtatg ttttgttcaa cctggatttg gagcgcgcct ccaccaaagc
    34441 cggatatacc tacccgtagg ccggataagc cgcgcctggc ggcgcatccg gcaagacaaa
    34501 ccgcaccaaa gccggtaatt caaaaatcac ccattcaggt gattcccagc caccagcaat
    34561 gcgcgaaaaa tcgacaatgt tcctctggaa caaatgaaac gtaacctgat aaaggtggta
    34621 aaaatgaagc cttattttgc tgctttgatg ttatcagtct ctgttcttcc tgcttatgct
    34681 ggtccgctcg gtactgctga taaagccgac ctaccgcaaa gcaatgtctc cagcccaatg
    34741 atggcccagt cgctcaggca accagacctg caaccgatat cgacggacag gaagacagaa
    34801 tgttttcgcc tttacacacc agatcgtaaa ccgggggtga actgcgttcc tgatggctca
    34861 acaggtcatt aagaatgagg gtgccagtga gtgcgaaaac tggcaccttt cccatgactt
    34921 agcggaaatt tacgctacgg tcgctggtca tgtcatacaa ctggaactta cgcccaagtt
    34981 gctgaccacc gtcacgcgtc agcggcgtcc agccaatcgc cgcacggctg cgtgtcgggc
    35041 cagacgagaa gagatccaac ggtaccgaga catacacgcc tttggtgaag tccccttcgc
    35101 cgtactcctc tttcgaaacg ttggtgatcg tggcatagcc acccaccacc acgccgctgt
    35161 caaagcgttt agcgatctcc agcgtgccgc ctttatcccc tgccagatac tgcccgacgc
    35221 tggctttcac caacacgtcc tgggcgaaag atggcgtcca gtaggcggtc agatgtccgg
    35281 ttttcacgct gtagtcggtg aatttcatca tatcttttgc actacgccag tcgcgctgtt
    35341 taacgtagtt ggcatccaga ccaaacgccc agttactgtc cagcggacga tacaacactt
    35401 cggcccccgc accgccaaac atggtttcga gataacctcc atagacctga ccgtagaagc
    35461 cgttgcccag atgctggaag tagttggctt gcaggttatt cacatagaca tcgttctgca
    35521 catactcgcg cacatgggta cgaacgcgcg gtaagtgcga gtcctgcggt ggattggtgt
    35581 agttaaactt gtcgtagttg ttggcaagat ttgcaaacag gctgccggtg gtcagtagat
    35641 ggtccgtcag ccacaaatct gccgttccca tcacgcccag ctgatacatg taaaagtttt
    35701 ccgggccacc gaccgactgg ttcagcaccg gatcgatatg gaaatcaaag cgtgatttgt
    35761 cgatatacca gccctgctcg gtggacttcg gaaccactgg ctcgacgcgt ttgtgcgcca
    35821 gcgtcgtttc gtggcccaac ggctctccgg cgagatgatt tttcaggctg gcgacatcgg
    35881 tttccgtcgt cacttgcggc atgttaaggc gattttccgt aatgcggatc gttttgatcc
    35941 catccggcag atcgttcatc acgatccgat tagcacggat gatcccttcg cgcgaatcac
    36001 gatatttcac ctgctcgccg gtaacataca gcgtatcgcc tttcgcctgg atctgcggat
    36061 cggcaagtcc ggcgttgtat ttcagcaacg ttaactgatt cgccaccacc gaatgctgca
    36121 aaatggcatc ctgcggctgc ggttgatatt gcgggcgggc gttatcgatg taggacgggc
    36181 gcagatcgtt aaagttggtg cgcagcgtga cgccaaacat aaaggtgttg ccacgttcat
    36241 agctaaggtt aacgtcggcc caatcggtaa cgcgataaat cgccccgacg ttaaacttac
    36301 ttttttgctc cagcttcccg gcaaaatcct gctgataatt attaccttca tactccagtt
    36361 tcagacgcag cggttgccag ggcgtctggt attccacgcc gccaaacagt gaggcaggac
    36421 cgtggaacat ctggctaccg tcgatagatc ccgcctgttt gtagctgtta tcgcgatagc
    36481 aatatttatc actggctgaa cagagcggat tttttacatt accgctggtg cccagatatc
    36541 cccagcccag gccgagcgta aaatcgaacg gcccccaggc tttgctggca acaagatatt
    36601 ccgcatcaaa cagccccgta ccgccgatat cccgcgcacc aaccgccact tgcggcagcc
    36661 agtaactctc ttcccacaaa cgcagtttga gatcgaaggc tttatcttta tacgtttgat
    36721 cgccagagaa cgcttcgacg ctgctgtact gccgggtgcg cacgtcggtg tagcgcagcg
    36781 ttgtttccag ccacgggaag agttgcactg aagctgagta ataacggtac tgatcgttat
    36841 cgcgatagtt cagactcaac tccccttccc gtgccatacg cgcggtggga gtttgtaata
    36901 atcctacgcc accgaaatcc gactgcgacg gaccaatggg cgcaggatat gtttcgccgt
    36961 agcaagctgt gctaatgccc agcgccagta agctaagcag atgtcttttt ttcattattg
    37021 gggtatccgc tgcgtcagag tctgaagaat gtcggcgtta agggcatcag gcgtctcact
    37081 ccagacggaa tctgcgaggc caacataaat aatgctgccg ggcatcggct ctacgtgacg
    37141 cttgttccag taagccaccg gcgctttttg cgtgcgtccg tccgggtaaa cgacccacgc
    37201 gtagctgcga tccgcaccgc tgagcaggct ctggtcagag agatagctcg ccacgtcgcg
    37261 acctggagtg aatggctgat tgccaggatg gctgataagc ccgaacaacg tgaccgtgga
    37321 cggcggtggt ccgacccaca gcgtgtagtt gccctgcaac ggcgggttac cgcgttcggc
    37381 aacgcggacg atatcgggat caagattgat tttttgcctg cctgtcactt tcaatgcctg
    37441 gatttgctgg cgtaaggcgt taatggcggc ggcatcgtcg gtgctggaat ctgcgccctg
    37501 ttctgccagc cgtgtcagca acgcctgctg ctgacgcaat gctgccgccg ttgccagctc
    37561 ttcgctaatc accgcaccag gccaccagct gtttgccagt cgaggttgcc ctaccagatc
    37621 gattaaatgc tctgcgcccg ttagcgtttt ggcctcactg ctgccattgc tgaacacctt
    37681 gacggttcct gccgcgaaga ccgatgacgc tcccacactc aaaagcaacg cgacaatagt
    37741 ttgtttaatc attgttttgc cgccttgatg agagtggttt tcaccgggaa ataatccgcg
    37801 ccgagatact gttccgactg gcgaatttgc ccttcgctgt cgatccagta gcggttatgc
    37861 cagcgcgcct ggtcggtgga gacttcttcg tccagcacgc gaaccagggt ttcatcgctg
    37921 ccgaccttga cggtatcggt gccatcccat ttgaagactg agcgtgcggt ggcgtagcgt
    37981 acctgctggt actcggtcca gcccatcgtg cgcgtccatg ttgcaccatc aacaatttgt
    38041 gcgggtttaa tcagcgggtc ggcggcaagg ttattcactt caatcaggtt gtcgccgcca
    38101 agcaaagtct tcaccagacg accatgttgt gtgacaagag tggcttgatc ctgagtgacc
    38161 catttttgtt gtccgtcttc agcgaaggcc agcaccacaa acaattgcgg cccgccatta
    38221 agctgcatgt actggctggc gtagggcata ttttgaatat catcgtccgt cagctgtacg
    38281 cctggcgtgc cgaacagact gtcccacagt gaattgccca gctctttagt cgtggctgaa
    38341 caggcctgta atagcaggca gataagaatg agtgcaggtc gcttcacgac tcttctccag
    38401 aggggcagaa taaccacacc ggagtgtggt tatggtaaat cccttcatac ttcaagccac
    38461 agatgcaatg actcaaatta ttttggggat acatacttta ttactgggta ctggtggtcg
    38521 tggttgtggt ggtcccggta ttagaaccat caccgccacc ggttgccgcg agcgcgacac
    38581 ccaccgccga gcttacggtg ctgacgctgg tcgcggagga actccccgcc gaaaccgacg
    38641 ttgctgccga ctctgccgct tcgcccacct gtacaggtgc agcccacgca gaagtcgccg
    38701 caagcgcaga tatggcaaaa atgccataca gaactttttt cataacaatt tcccttcatt
    38761 gaatgaatgg agatttaccc aaaaacattt cgggcgggat cgagtataca caactaaagc
    38821 atgcgaatta cggcgcgggg aattagcgat ggagttttag gataattgga atgaatatca
    38881 aatataagtt tcaacttata tttaaataac aataaaaatc ataagcttaa aataaaaaac
    38941 aaaagtaatt aatctgcgta ttctccgaaa taaacaacga ttaaaaatct attttaggat
    39001 taacctcacg gtatgatttc cgttaaatca cagacaaggg gatttatctg ataaaacaat
    39061 gccgacttat tgtcggcatt gtttttaagg ttatttaact cctcatacta tgcattaatg
    39121 tcgatattgg catcaggctc cggttgccgg atgcggcgta aacgccttat ccggcctaca
    39181 tatcggcatc aggcacgcat gatgattaac cgcgccacgc tttatagcgg ttaatcagac
    39241 cattggtcga gctatcgtgg ctgctgattt ctttatcatc tttcagctct ggcagaatac
    39301 ggtttgccag ttgtttaccc agctccacgc cccactggtc gaaggtgaag atgttcagga
    39361 tcacgccctg agtaaagatt ttgtgctcat acagcgcaat caacgcaccc aggctgaacg
    39421 gagtgatttc gcgcagcagg atggagttgg tcgggcggtt accttcgaac actttgaacg
    39481 gcaccacgta gtcaagcgtt gccggatctt taccctgatc gcgatattcc tgctcaacca
    39541 cttcgcggga tttaccaaac gccagcgctt cggtctgggc gaagaagtta gacagcagtt
    39601 tctggtggtg atcagagagc gggttatggg tgatagccgg ggcgatgaaa tcgcacggta
    39661 ccattttggt tccctggtgg atcagctggt agaacgcgtg ctgaccgtta gtgcctggtt
    39721 caccccagat aatcgggcca gtctggtaat ccacaacgtt accgttacgg tcaacatact
    39781 taccgttgga ctccatattg ccctgctgga agtacgccgc gaaacggtgc atatactggt
    39841 catacggcag aatcgcttca gtttccgcac caaagaaatt gttgtaccag atgccaatca
    39901 gcgccagcag gacaggcagg tttttctcgg caggcgtggt ggagaaatgc ttgtccattg
    39961 cgtgtgcgcc agaaagcagt tcaacgaagt tatcaaagcc gatggagaga acaatcgaca
    40021 ggccaatcgc tgaccacaaa gagtaacggc cgccgaccca gtcccagaac tcgaacatgt
    40081 tggcagtatc aataccaaac tcgccaacgg ctttggcatt ggttgagagc gccgcaaaat
    40141 gtttcgcaac gtgtttttca tcacctgccg ctttcaggaa ccagtcacgc gcgctatggg
    40201 cgttggtcat ggtttcctga gtggtgaagg ttttagatgc taccaggaac agcgtggttt
    40261 ccgggtttac ttttttcagc acttccgaga tgtgagtccc atcgacgtta gaaacaaagt
    40321 gcatgttcag gtggtttttg tacggacgca gtgcttcggt caccatgtac gggccgaggt
    40381 cagaaccgcc gatcccgatg ttcactacgt cagtgattgc tttgccggta taacccttcc
    40441 actcaccgga gataatcgct tccgagaagg ttttcatctt ctccagcacc gcgttgactt
    40501 ctggcataac atctttgcca tcaaccagaa tcggggtatt gctacgatta cgcagcgcta
    40561 cgtgcagcac ggcgcggttt tcagtgcggt tgatcttctc gccagagaac atcgacttaa
    40621 tcgcgcccgc cagatcgcac tctttcgcca gatcctgtaa tttcgccagc gtctcttcag
    40681 tgatgcggtt tttggagtaa tccaccagca tctgatcgtc gaaggttgcg gagaacttag
    40741 aaaaacgatc gccgtcttta gcaaaaagat cggcgatcgt aacgtctttc atttcatcga
    40801 agtgtttctg tagtgcctgc caggcagcgg tctgcgttgg attgatgttt ttcattagca
    40861 atactcttct gattttgaga attgtgactt tggaagattg tagcgccagt cacagaaaaa
    40921 tgtgatggtt ttagtgccgt tagtgtaatg ttgagtgtaa acccttagcg cagtgaagca
    40981 tttattagct gaactactga ccgccaggag tggatgaaaa atccgcatga ccccgtcgtt
    41041 gacaaccgcc ccgctcaccc tttatttata aatgtactac ctgcgctagc gcaggccaga
    41101 agaggcgcgt tgcccaagta acggtgttgg aggagccagt cctgtgataa cacctgaggg
    41161 ggtgcatcgc cgaggtgatt gaacggctgg ccacgttcat catcggctac aggggctgaa
    41221 tcccctgggt tgtcaccaga agcgttcgca gtcgggcgtt tcgcaagtgg tggagcactt
    41281 ctgggtgaaa atagtagcga agtatcgctc tgcgcccacc cgtcttccgc tcttcccttg
    41341 tgccaaggct gaaaatggat cccctgacac gaggtagtta tgtctgaaat tgttgtctcc
    41401 aaatttggcg gtaccagcgt agctgatttt gacgccatga accgcagcgc tgatattgtg
    41461 ctttctgatg ccaacgtgcg tttagtagtc ctctcggctt ctgctggtat cactaatctg
    41521 ctggtcgctt tagcggaagg gctggaacct ggcgagcgat tcgaaaaact cgacgctatt
    41581 cgcaacatcc agtttgccat tctggaacgt ctgcgttacc cgaacgttat ccgtgaagag
    41641 attgaacgtc tgctggagaa cattactgtt ctggcagaag cggcggcgct ggcaacgtct
    41701 ccggcgctga cagatgagct ggtcagccac ggcgagctga tgtcgaccct gctgtttgtc
    41761 gaaatcctgc gcgaacgcga tgttcaggca cagtggtttg atgtacgtaa agtgatgcgt
    41821 accaacgacc gatttggtcg tgcagagcca gatgtagccg cgctggcgga actggccgcg
    41881 ctgcagctgc tcccacgcct caatgaaggc ttagtgatca cccagggatt tatcggtagc
    41941 gaaaataaag gtcgtacaac gacgcttggc cgtggaggca gcgattatac ggcagccttg
    42001 ctggcggagg ctttacacgc atctcgtgtt gatatctgga ccgacgtccc gggcatctac
    42061 accaccgatc cacgcgtagt ttccgcagca aaacgcattg atgaaatcgc gtttgccgaa
    42121 gcggcagaga tggcaacttt tggtgcaaaa gtactgcatc cggcaacgtt gctacccgca
    42181 gtacgcagcg atatcccggt ctttgtcggc tccagcaaag acccacgcgc aggtggtacg
    42241 ctggtgtgca ataaaactga aaatccgccg ctgttccgcg cgctggcgct tcgtcgcaat
    42301 cagactctgc tcactttgca cagcctgaat atgctgcatt ctcgcggctt cctcgcggaa
    42361 gttttcggca tcctcgcgcg gcataatatt tcggtagact taatcaccac gtcagaagtg
    42421 agcgtggcat taacccttga taccaccggt tcaacctcca ctggcgatac gttgctgacg
    42481 caatctctgc tgatggagct ttccgcactg tgccgggtgg aggtggaaga aggtctggcg
    42541 ctggtcgcgt tgattggcaa tgacctgtca aaagcctgcg gcgttggcaa agaggtattc
    42601 ggcgtactgg aaccgttcaa cattcgcatg atttgttacg gcgcatccag ccataacctg
    42661 tgcttcctgg tgcccggcga agatgccgag caggtggtgc aaaaactgca tagtaatttg
    42721 tttgagtaaa tactgtatgg cctggaagct atatttcggg ccgtattgat tttcttgtca
//
LOCUS       DAJTRH010000026        39993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTRH010000026 DAJTRH010000000
VERSION     DAJTRH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..158
                     /locus_tag="OO339_001521"
     CDS             <1..158
                     /locus_tag="OO339_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5926199.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            387..977
                     /gene="uidR"
                     /locus_tag="OO339_001522"
     CDS             387..977
                     /gene="uidR"
                     /locus_tag="OO339_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5926200.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1365..3068
                     /gene="uidA"
                     /locus_tag="OO339_001523"
     CDS             1365..3068
                     /gene="uidA"
                     /locus_tag="OO339_001523"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5926201.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3074..4099
                     /gene="uidB"
                     /locus_tag="OO339_001524"
                     /pseudo
     CDS             3074..4099
                     /gene="uidB"
                     /locus_tag="OO339_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4138..5403
                     /gene="uidC"
                     /locus_tag="OO339_001525"
     CDS             4138..5403
                     /gene="uidC"
                     /locus_tag="OO339_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5926202.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5629..7137)
                     /locus_tag="OO339_001526"
     CDS             complement(5629..7137)
                     /locus_tag="OO339_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5926203.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7238..8413)
                     /gene="manA"
                     /locus_tag="OO339_001527"
     CDS             complement(7238..8413)
                     /gene="manA"
                     /locus_tag="OO339_001527"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5926204.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8612..10258
                     /gene="fumB"
                     /locus_tag="OO339_001528"
     CDS             8612..10258
                     /gene="fumB"
                     /locus_tag="OO339_001528"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5926205.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10401..11804
                     /gene="fumC"
                     /locus_tag="OO339_001529"
     CDS             10401..11804
                     /gene="fumC"
                     /locus_tag="OO339_001529"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5926206.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11801..12730)
                     /gene="tus"
                     /locus_tag="OO339_001530"
     CDS             complement(11801..12730)
                     /gene="tus"
                     /locus_tag="OO339_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5926207.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12806..14107)
                     /gene="rstB"
                     /locus_tag="OO339_001531"
     CDS             complement(12806..14107)
                     /gene="rstB"
                     /locus_tag="OO339_001531"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5926208.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14286..15605)
                     /locus_tag="OO339_001532"
     CDS             complement(14286..15605)
                     /locus_tag="OO339_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5926209.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15830..16573)
                     /gene="rstA"
                     /locus_tag="OO339_001533"
     CDS             complement(15830..16573)
                     /gene="rstA"
                     /locus_tag="OO339_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5926210.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16702..17037
                     /locus_tag="OO339_001534"
     CDS             16702..17037
                     /locus_tag="OO339_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5926211.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17034..17756)
                     /gene="folM"
                     /locus_tag="OO339_001535"
     CDS             complement(17034..17756)
                     /gene="folM"
                     /locus_tag="OO339_001535"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5926212.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17793..19175)
                     /locus_tag="OO339_001536"
     CDS             complement(17793..19175)
                     /locus_tag="OO339_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5926213.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19361..20305)
                     /locus_tag="OO339_001537"
     CDS             complement(19361..20305)
                     /locus_tag="OO339_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5926214.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20829..22361
                     /gene="pntA"
                     /locus_tag="OO339_001538"
     CDS             20829..22361
                     /gene="pntA"
                     /locus_tag="OO339_001538"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5926215.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22372..23760
                     /gene="pntB"
                     /locus_tag="OO339_001539"
     CDS             22372..23760
                     /gene="pntB"
                     /locus_tag="OO339_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5926216.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23785..24819)
                     /gene="tqsA"
                     /locus_tag="OO339_001540"
     CDS             complement(23785..24819)
                     /gene="tqsA"
                     /locus_tag="OO339_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5926217.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25230..25595
                     /gene="mdtJ"
                     /locus_tag="OO339_001541"
     CDS             25230..25595
                     /gene="mdtJ"
                     /locus_tag="OO339_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5926218.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25582..25911
                     /gene="mdtI"
                     /locus_tag="OO339_001542"
     CDS             25582..25911
                     /gene="mdtI"
                     /locus_tag="OO339_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5926219.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25950..26771)
                     /locus_tag="OO339_001543"
     CDS             complement(25950..26771)
                     /locus_tag="OO339_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5926220.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26871..26954)
                     /locus_tag="OO339_001544"
     CDS             complement(26871..26954)
                     /locus_tag="OO339_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926221.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27047..27382)
                     /locus_tag="OO339_001545"
     CDS             complement(27047..27382)
                     /locus_tag="OO339_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5926222.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27779..29032)
                     /locus_tag="OO339_001546"
                     /pseudo
     CDS             complement(27779..29032)
                     /locus_tag="OO339_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29139..30050
                     /locus_tag="OO339_001547"
     CDS             29139..30050
                     /locus_tag="OO339_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5926223.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30185..31405
                     /gene="mlc"
                     /locus_tag="OO339_001548"
                     /gene_synonym="dgsA"
     CDS             30185..31405
                     /gene="mlc"
                     /locus_tag="OO339_001548"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5926224.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31530..32225
                     /gene="bioD"
                     /locus_tag="OO339_001549"
     CDS             31530..32225
                     /gene="bioD"
                     /locus_tag="OO339_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5926225.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32178..33470)
                     /gene="clcB"
                     /locus_tag="OO339_001550"
     CDS             complement(32178..33470)
                     /gene="clcB"
                     /locus_tag="OO339_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5926226.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33629..34243)
                     /gene="dmsD"
                     /locus_tag="OO339_001551"
     CDS             complement(33629..34243)
                     /gene="dmsD"
                     /locus_tag="OO339_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5926227.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34286..35140)
                     /locus_tag="OO339_001552"
     CDS             complement(34286..35140)
                     /locus_tag="OO339_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5926228.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35142..35759)
                     /gene="dmsB"
                     /locus_tag="OO339_001553"
     CDS             complement(35142..35759)
                     /gene="dmsB"
                     /locus_tag="OO339_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5926229.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSVAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35770..37808)
                     /locus_tag="OO339_001554"
                     /pseudo
     CDS             complement(35770..37808)
                     /locus_tag="OO339_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37869..38978)
                     /locus_tag="OO339_001555"
     CDS             complement(37869..38978)
                     /locus_tag="OO339_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5926230.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38961..>39993
                     /locus_tag="OO339_001556"
     CDS             38961..>39993
                     /locus_tag="OO339_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5926231.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVES"
BASE COUNT        10168 a         9699 c        10243 g         9883 t
ORIGIN      
        1 aaattgaaca aaaaatgtta cagcacacgc cgatcagacg tctgggccaa ccgcaagata
       61 ttgctaacgc ggcgctgttc ctttgctcgc ctgcagccag ctgggtaagc ggacaaattc
      121 tcaccgtctc cggtggtggg gtacaggagc tcaattaata cactaacgaa ccggtaaacg
      181 gtcgtatgtg ttgtttaccg ggatagcgtg tgcaattcat caacgtctct gctaaacaac
      241 tggcagccaa atcacggcta ttggttaacc aattgctggg tgaaaagtat acgaatagag
      301 tgtaccttcg cactattcaa cagcaatggt aggcgctcac ctgacaacgc ggtaaactag
      361 ttattcacgc taactataat ggtttaatga tggataacat gcagactgaa gcacaaccga
      421 cacggacccg gatcctcaat gctgccagag agattttttc agaaaatgga tttcacagtg
      481 cctcgatgaa agccatctgt aaatcttgcg ccattagtcc tgggacgctc tatcaccatt
      541 tcatctccaa agaagccttg attcaggcga ttatcttaca ggaccaggag agggcgctgg
      601 ctcgtttccg ggaaccgatt gaagggattc attttgttga ctatatggtc gagtctattg
      661 tctctctcac ccatgaagcc tttgggcaac gggcgctggt ggtcgaaatt atggcggaag
      721 ggatgcgtaa cccacaggtc gccgccatgc ttaaaaataa gcatatgacg atcactgaat
      781 ttgttgccca gcggatgcgt gatgcccagc aaaaaggcga gataagccca gacatcaaca
      841 cggcaatgac ttcacgttta ctgctggatc tgacctacgg tgtactggcc gatatcgaag
      901 cggaagacct ggcgcgtgaa gcgtcgtttg ctcagggatt acgcgcgatg attggcggta
      961 tattaaccgc atcctgatcc tctcatttcg cgggctggtg ataactgtgc ccgcgtttca
     1021 tatcgtaatt tctctgtgca aaaattatcc tccccgtgtt cggagaattc ccccccaaaa
     1081 tattcaccgt agccatatgt catgagagtt tatcgttccc aacactctcg aacgaacgtt
     1141 cgattgctta ttttatggct tctgtcaacg ctgttttaaa gattaatgcg atccatatca
     1201 cgctgtgggt attgcagttt ttggtttttt gatcgcggtg tcagttcttt ttatttccat
     1261 ttctcttcca tgggtttctc acagataact gtgtgcaaca cagaattggt taactaatca
     1321 gattaaaggt tgaccagtat ttttatctta atgaggagtc ccttatgtta cgtcctgtag
     1381 aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg
     1441 aaaactgtgg aattgatcag cgttggtgcg aaagcgcgtt acaagaaagc cgggcaattg
     1501 cagtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgtgggca
     1561 acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc
     1621 tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg
     1681 agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga
     1741 aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg
     1801 gaatggtgat taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta
     1861 actatgccgg gatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg
     1921 atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg
     1981 tggtggccaa tggtgatatc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa
     2041 ctggacaagg caccagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg
     2101 aaggttatct ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt gatatctacc
     2161 cgctgcgcgt cggcatccgg tcagtggcag tgaagggcga acagttcctg attaaccaca
     2221 aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggatttgcga ggcaaaggat
     2281 tcgataacgt gctgatggtg cacgatcacg cattaatgga ctggattggg gccaactcct
     2341 accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca
     2401 tcgtggtgat tgatgaaact gcagctgtcg gctttaacct ctctttaggc attggtttcg
     2461 aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc tgtcaacggg gaaactcagc
     2521 aggcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg
     2581 tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg
     2641 cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg
     2701 taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga
     2761 accgttatta cggttggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg
     2821 aaaaagaact gctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg
     2881 gcgtggatac gttagccggc ctgcactcaa tgtacaccga catgtggagt gaagagtatc
     2941 agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg
     3001 aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta
     3061 acaattgacc tacatgggcc tcgggctttg ctacagcctg gtgaatattc cttatggttc
     3121 acttgctacc gcgatgaccc aacaaccaca atctcgcgcc cgtctgggcg cggctcgtgg
     3181 gattgccgct tcattgacct ttgtatgcct ggcatttctg ataggaccga gcattaagaa
     3241 ctccagcccg gaagagatgg tgtcggtata ccatttctgg acgattgtgc tggtgattgc
     3301 cggaatggtg ctttacttca tctgcttcaa atcgacgcgt gaaaatgtgg tacgtatcgt
     3361 ggcgcagccg tcattgaaga tcagtttgca aaccctgaaa cggaatcgcc cgctgtttat
     3421 gttgtgcatc ggtgcgctgt gtgtgctgat ttcgaccttc gcggtcagcg cctcgtcgtt
     3481 gttctacgtg cgctatgtgt taaatgatac cgggctgttc actgtgctgg tactggtgca
     3541 aaacctggtt ggtactgtgg catcggcacc gcttgtgccg gggatggtcg cgaggatcgg
     3601 taaaaagaat accttcctga ttggcgcttt gctgggaacc tgcggttatc tgctattctt
     3661 ctgggtttcc gtctggtcac tgccggtggc gttggttgcg ttggccatcg cttcaattgg
     3721 tcagggcgtt actatgactg tgatgtgggc actggaagct gataccgtag aatacggtga
     3781 atacctgacc ggcgtgcgaa ttgaagggct cacctattca ctattctcat ttacccgtaa
     3841 atgcggtcag gcaatcggag gttcaattcc tgcctttatt ttgggattaa gcggatatat
     3901 cgccaatcag gtgtaaacgc cggaagttat tatgggcatc cgcacatcaa ttgccttagt
     3961 accttgcgga tttatgctac tggcattcgt tattatctgg ttttatccgc tcacggataa
     4021 aaaattcaaa gaaatcgtgg ttgaaattga taatcgtaaa aaagtgcagc agcaattaat
     4081 cagcgatatc actaattaat attcaataaa aataatcaga acatcaaagg tgcaactatg
     4141 agaaaaatag tggccatggc cgttatttgc ctgacggctg cctctggcct tacctctgct
     4201 tacgcggcgc aactggctga cgatgaagcg ggactacgca tcagactgaa aaacgaattg
     4261 cgcagggctg ataagcccag tgctggcgcg ggaagagata tttacgcatg ggtacaggga
     4321 ggattgctcg atttcaatag tggttattat tccaatattg ttggcgttga aggcggagcg
     4381 tattatgttt ataaattagg tgctcgtgct gatatgagta cccggtggta tcttgatggt
     4441 gataaaagtt ttggctttgc cctgggggca gtaaaaataa aacccagtga aaatagcctg
     4501 cttaaattag gtcgcttcgg gacggattat agttatggta gcttacctta tcgtattccg
     4561 ttaatggttg gcagttcgca acgtacatta ccgacagttt ctgaaggagc attaggttat
     4621 tgggctttaa cgccaaatat tgatctgtgg ggaatgtggc gttcacgagt atttttatgg
     4681 actgattcaa caaccggtat tcgtgatgaa ggggtgtata acagccagac gggaaaatac
     4741 gataaacatc gcgcacgttc ttttttagcc gccagttggt atgatgatac cagtcgctat
     4801 tctctggggg catcggtaca gaaagatgtt tccaatcaga tacaaagtat tctcgagaaa
     4861 agcataccgc tcgacccgaa ttatacgttg aaaggggagt tgctcggctt ttacgcgcag
     4921 ctcgaaggtt taagtcgtaa taccagccag cccaatgaaa cggcgttggt tagtggacaa
     4981 ttgacctgga atgcgccgtg gggaagcgta tttggcagtg gtggttattt gcgccatgca
     5041 atgaatggtg ccgtggtgga taccgacatt ggctatccct tttcattaag tcttgatcgt
     5101 aaccgtgaag gaatgcagtc ctggcaatta ggtgccaact accgtgtaac tccgcaattt
     5161 acgctgacat ttgcaccgat tgtgactcgc ggctatgaat ccagtaaacg agatgtgcgg
     5221 attgaaggcg cgggtatctt aggtggtatg aactatcggg tcagcgaagg gccgttacaa
     5281 gggatgaatt tctttcttgc tgccgataaa gggcgggaaa agcgcgatgg cagtacgctg
     5341 ggcgatcgcc tgaattactg ggatgtgaaa atgagtattc agtatgactt tatgctgaag
     5401 taagaaataa cgccggagag aaaaacctcc ggcgtttcag attgttgaca aagtgcgcgt
     5461 tttttatgcc ggatgcggcg taaacgcctt atccagccta caaaaactca taaattcaaa
     5521 gtgttgcagg aaaaggtagg cctgataagc gtagcgcatc aggcaatctc tggtttgttt
     5581 tcagatgaaa acgccggagt gaaaattctc cggcgttttg gccgtgaatt actgctgcgg
     5641 aattgccggt acagccggaa cgttaagagc tggcatcgca aacatgccaa caaaatcttc
     5701 taacgacatt ttctgcccat ttaacgttat ctgaccgtta gcatattgca ggctggtggt
     5761 gatggtattg tcctgcaagg tggtcagacg gaacatctgc cccattgctg atgcaccttc
     5821 aacttgctgt ttcgccagtt ttttcgcttg atcttcctga taaccttcca gcttcgctac
     5881 ctgagtcatc aactcagttg ccatatccac cggaatggtc agtttcgcat ccagagattt
     5941 aaccgaacga tctacttcct gcgccagcgt ttgcggcgct tctttagtcg ttgccggatc
     6001 tttcaggaac agcgacagat tcagcgcact ttcaccctga ctgtttttcc agcttagcgg
     6061 cgcgatagta atcaccggat cgcctttcaa catcagcggc agggcgctaa agaaggcttc
     6121 cgtcactttc tcctgataaa gttcgggatt gttggcaatt tctggctgtg ccagcagcgc
     6181 ctgagtttgc gcgttatatt gctggctaaa ctgatgccag gcttcaccat caatctggcc
     6241 gacttttaaa gtcagcttgc cgctgcccag atcctgattc tgtaccttca ggctgtttag
     6301 cgagtaatcc agttggctat tgatcgtttt accgtcattg accagatccg atttaccgct
     6361 gatctccatg ccttccagca gtgccagttc tttgccttcc actgaaatgg tcattttttc
     6421 cagtgacagt ttttggtttc ctacgcgctc accaaaactt cccagcgtgc tggaaccgtc
     6481 ggttttcaga ttattaaagg tcaactgcac tttctggttg tattcgttaa ctgcgtctat
     6541 ccgaccactt tgcgcctctc cggaaaggga gatggctttg ccgtctctgt cagcatttaa
     6601 ctggaactcg ccgccgctaa aggcgacttt ttcatccttt tgctcgtaat tcagtggatt
     6661 gagcgaaata tcggaactgg aatcaccgct gtaaccaatg cgcgagttaa tctcaaaagg
     6721 cgtttcacct tttgccatat caaacagtgg tttgcttact tcgttattaa ccagcgtggt
     6781 ttgaattgat gccatcgacg ggatcaggtt cagtttttta agctgggcaa gcgggaaggg
     6841 accatgatca accgattcgt tgaagatgac gctctgaccg cttttaatcc acggattttc
     6901 tttcccggca atgggtttca ccaacagttg caactggctg ctgaatacgc cgcgatgata
     6961 gttttgataa ctcacttcca ggttggattc aggagctgtc agtttgagtt gcgcgttcgc
     7021 ctgcgcgacc atgtcttcga gatgggtttc aatcttcttg cctgtatacc atgcgccgcc
     7081 tgtccagact acgcctagcg caacaatgac gcctaccgct accagcgatt tattcataat
     7141 gattatccat aaaatgaaat caggcggact ggccgcctga aggtgttata agcctttaat
     7201 aagcttagca agagatgtta attttttcag taagctctta cagcttgttg taaacacgcg
     7261 ctaaacggcc gtggcctttg acagtcaccg gtgattcgtt ggcggcaata aacgctgatt
     7321 cacccggttt aagctgtaac tgctgagaac ctttccacaa cgttgcatcg ccttcgacgc
     7381 agaacaaaat ggcggcactc tgctggctga tggtggtttc tttatcacta aggtcatgca
     7441 gcgagaaggc aaaatcatcc acaggaatcg ggaagtccag ttctgcacct tgtttcaccg
     7501 gctgggtcaa caactggtta gccggtttgg cttcgaattt cacattggca accagttccg
     7561 gaatatcaat gtatttaggc gtcagacctg cacgcagcac gttatcggag ttcgccatca
     7621 cttccagcgc cacgccttgc aggtaagcgt gcggtgtttc agcgaacagg aacatcgctt
     7681 cgccaggatt caatttcacc acattcagta acagcgggga gaacaggccg ctgtcttccg
     7741 ggtaaaattc agaaattaaa cgaatcgttt gccacggttc accctgctga ctgtcgagcg
     7801 ccgattttaa aatcgccagc gcgcgggatt tttcttcacc ctgcatattc aacaggttgg
     7861 cgaacagttc gcttaaacgt tcggcatgtg gctgttgtaa aaagtgagca atcgccggat
     7921 gtgcaccggc gaccggctgg agtagggaga caatctcgga aaattcacga aacgcgttca
     7981 tcgcaaggaa aggcgtcagc gcaaaaacca gctccggctt gtggttagga tctttatagt
     8041 tacgctcggc ggcatccatc gggatacctg cggcattttc tttggcaaaa ccgatttcag
     8101 aattgtgttt gtttggatga acctgaatgg agagtggctg tgctgcgcat aatactttga
     8161 acaggaaagg cagttcgcca aagcgtttgg caacggcctc tccgagcaga gtcgatttat
     8221 cactctcaat cacatcacgc agtgaaacga tatctccggc ggcattctgc actcgtgaac
     8281 tgcttttcgg atgtgcgccc atccacagct cggccatcgg ctggctggac ggattttcca
     8341 taccataaag ttcagtcaac gccgttttgc taccccaggc atagttttgc actgagttaa
     8401 tgagtttttg catgatcaat ccctgtttta atgtggaaat taatcccgct attaaagcaa
     8461 gaatcctacg ggaagtaacc tggagccgca aaaagtcgta ctagtctcag tttttgttaa
     8521 aaaactgtgt aggatattgt tactcgcttt taacagggca acggaacacc cgcccagagc
     8581 ataaccaaac caggcagtaa gtgagagaac aatgtcaaac aaaccctttc attatcagtc
     8641 tccttttcca ctcaaaaaaa atgatactga gtattacctg ctaaccagtg aacacgttag
     8701 cgtatctgaa tttgaagggc aggagatttt gaaagtcgca cccgaagcgt taactctgtt
     8761 ggcgcgtcag gcgtttcatg atgcgtcgtt tatgctgcgt ccggctcacc aacaacaggt
     8821 ggccgacatt ctgcgtgacc cggaggccag cgaaaatgat aaatatgtgg cgctgcaatt
     8881 cctgcgtaac tccgacatcg cggcgaaagg cgttctgcca acctgtcagg ataccggcac
     8941 cgcgattatt gttggtaaaa aagggcagcg tgtatggacc ggtggtggtg atgaagcggc
     9001 gctggcgcgc ggtgtctata acacttatat cgaagataat ctgcgctact cgcaaaacgc
     9061 gccgctggat atgtataaag aagtgaatac cggcaccaat ctgccagcgc agatcgatct
     9121 ttatgccgtt gatggcgacg aatacaaatt cctctgtatc gcaaaaggtg gtggttcggc
     9181 aaacaagacg tatctctatc aggaaaccaa agcgttactg acgccgggga aactgaaaaa
     9241 ttacctggtt gagaagatgc gcacgctggg taccgcggcc tgtcctccgt atcatattgc
     9301 gttcgttatt ggtggaactt ctgcagaaac gaaccttaaa acggtgaaac tggcttccgc
     9361 gaaatactat gatgaactgc caacggaagg gaatgagcac ggtcaggcgt tccgcgatgt
     9421 ggaactggaa aaagaattac tgatcgaagc gcaaaatctc ggtctgggtg cgcagtttgg
     9481 tggtaaatac ttcgctcacg acatccgcgt gattcgcctg ccacgtcacg gcgcatcctg
     9541 tccggttggt atgggtgttt cctgctctgc tgaccgtaat atcaaagcga agatcaaccg
     9601 tcaggggatc tggatcgaaa aactggaaca taatccaggt aaatatatcc ctgaagagct
     9661 gcgtaaagcg ggagaaggcg aagcggtgcg cgttgacctt aaccgtccga tgaaagagat
     9721 cctcgcacag ttgtcgcagt atcccgtttc tacacgctta tcgcttaacg gcacgattat
     9781 cgttggtcgt gatattgctc acgccaaact gaaagagcgg atggataacg gtgaagggct
     9841 gccgcaatac attaaagatc atccgattta ctacgcgggt ccggccaaaa cgccggaagg
     9901 ttatgcctcc ggttctcttg gcccaacgac cgccggacgg atggattctt atgtcgatca
     9961 actgcaagcg cagggcggaa gtatgatcat gctggcgaaa ggcaaccgca gccagcaggt
    10021 gacggatgcc tgtaaaaaac acggcggctt ctaccttggc agtatcggtg gtccggccgc
    10081 tgtattggcg cagggaagta ttaagagcct ggaatgtgtt gaatatccgg aactgggaat
    10141 ggaagccatc tggaaaattg aagtggaaga tttcccggcg tttatccttg tggatgataa
    10201 aggaaatgac ttcttccagc agatacaact cacacagtgc actcgctgtg tgaaataaac
    10261 agagccgccc ttcggggcgg tttttttaca tggcacgaaa gaccaaacat ttgttatcaa
    10321 atagtaaata atgagtgagc taaaagttgc ttaacgaaag caaaacagaa agaaaaaatt
    10381 aatcaggtga ggagcaggcc atgaatacag tacgcagcga aaaagattcg atgggggcga
    10441 ttgatgtccc ggcagataag ctgtggggcg cacaaactca acgctcgctg gagcatttcc
    10501 gcatttcgac ggagaaaatg cccacctcac tgattcatgc gctggcgcta accaagcgcg
    10561 cagcggcaaa agttaatgaa gatttagact tgttgtctga agagaaagcg agcgccattc
    10621 ggcaggcggc ggatgaagta ctggcaggac agcatgacga cgaattcccg ctggctatct
    10681 ggcagaccgg ctccggcacg caaagtaaca tgaacatgaa cgaagtgctg gctaaccggg
    10741 ccagtgaatt actcggcggt gtgcgcggga tggaacgtaa agttcaccct aacgacgacg
    10801 tgaacaaaag ccaaagttcc aacgatgtct ttccgacggc gatgcacgtt gcggcgctgc
    10861 tggcactgcg caagcaactc attccgcagc ttaaaaccct gacacagacg ctgagtgaaa
    10921 aatcgcgtgc atttgccgat atcgtcaaaa tcggtcgaac ccacttgcag gacgccacgc
    10981 cgctaacact agggcaggag atttccggct gggtagcgat gctcgagcat aatctcaaac
    11041 atatcgaata cagcctgcct cacgtagcgg aactggctct tggcggtaca gcggtgggta
    11101 ctggactaaa tacccatccg gagtatgcgc gtcgcgtagc agatgaactg gcagtcatta
    11161 cctgtgcacc gtttgttacc gcgccgaaca aatttgaagc gcttgcgacc tgtgatgccc
    11221 tggttcaggc gcacggcgcg ttgaaagggt tggctgcgtc actgatgaaa atcgccaatg
    11281 atgtccgctg gctggcctct ggcccgcgct gcggaattgg tgaaatctca atcccggaaa
    11341 atgagccggg cagctcaatc atgccgggga aagtgaaccc aacccagtgt gaagcattaa
    11401 ccatgctctg ctgtcaggta atggggaacg acgtggcgat caacatgggt ggcgcttccg
    11461 gtaactttga actgaacgtc ttccgtccga tggtgatcca taatttcctg caatcggtgc
    11521 gcttgctggc agatggcatg gaaagtttta acaaacactg cgcagtgggt attgaaccga
    11581 atcgtgagcg aatcaatcaa ttactcaatg aatcgctgat gctggtgact gcgcttaaca
    11641 cccacattgg ttatgacaaa gccgcggaga tcgccaaaaa agcgcataaa gaagggctga
    11701 ccttaaaagc tgcggccctt gcgctggggt atcttagcga agccgagttt gacagctggg
    11761 tacggccaga acagatggtc ggcagtatga aagccgggcg ttaatctgca acatacaggt
    11821 gcagccgtgg aatgatcaaa cgaagcggct gcgcctgagg tttataacgg tgctgcacat
    11881 tgtcggcatc gtaatttaac aactcaccaa cgtccggcac gcccgcgcca ttatcccgat
    11941 taatcagtgc aatcagtggt gtagggcagg cgtgttggac ttgtttttga tctcctttgt
    12001 accagacgcg ggcaatcggc tgcaccttca ctggacgttt gatttttaac ttcgcgttct
    12061 gtggcagggc agcgatatcc tgatactctc gctccagttt tctttgccac tcctcgcgcg
    12121 tccacggtgc gacactgcgt ggtgatttca ggcttttttc cagctgtgcc aggacttcat
    12181 cacgatgcaa attcttaatg atatgtttat tagcccaacc aaagcgtaaa gtggcggggt
    12241 cgtgcagaac ggtgagcgtg cggtaagcat taagggtgat cagccccggc aaatgacgat
    12301 gcacccattc aaaacgtgcc gcggtgggga gttctgattc aaccgtgacg atatgctcga
    12361 acgtggtctt gagtttattg atgtgctgaa tatgactgac caacgctgct tgcgaaaggt
    12421 tatcgacctg gtaacacaac acgccaggca gacggacagc ggccttgctg ctgcgatttt
    12481 cggactgttg ttgaataaat aaatggcgga aatgacgcaa cgccagcgac tgcgcgtcgt
    12541 tgccgagatg ttgttttacc tcaatacgat taagcggatt atgctcatcc tcttttttta
    12601 cctccggcaa agagaacacg cgggcaacca atagcttgtg ttgctcaaga tgagcggcaa
    12661 atgtagccag ctcttgttcc atctggcgaa aggtagtgtt gagtcggtct acgagatcgt
    12721 aacgcgccat aatattaacc actttagtta caacatactt attttatagc acagtcgtgg
    12781 tgacttgacc atcgtgcgcg tagtgtcagg cagaggtaaa ttgcgggatg ttatgccata
    12841 acggccagct aaacgagaag cgagcaccac ccagttcgct gatgtcacaa ttaaccgtac
    12901 cgcccattgt cagtgctatt gagtggacaa ttgccagccc cagcccgcag ccgccggttg
    12961 agcgatcccg gctgggatcg aggcgaacaa aaggttcaaa gatatgttcg cggttttctg
    13021 gggcaatccc tgggccatca tcctcaacaa ttaatgtcgc tctattcccc gacagtagca
    13081 ggctggtttc aaccgttgaa tggcagtagc gcagggcgtt attgagcaaa ttatccagca
    13141 cgcgctccat taagcgcata tccaacgccg cataatggcc ttgcatgagc gtttttatcc
    13201 gtaccgtttt atcgggcgtt actgcctgaa tatctgccag atgcgttgac agccacaacg
    13261 gcaggtctgg ttcgctaaga tgaagctcgt tttgtgggcg atcgagtcgg gcataagtca
    13321 gcagctcttc aattaaagct tcaagttgac tgatatcacg attcaacgcc tgggattcgg
    13381 cggcgctcag gttatcgctc atctccagtc gataacgcag gcgcactaac ggtgttcgca
    13441 gttcgtgagc gataccgtca ataagctgtt ttttgctggc aattaaggcg ttgatattgt
    13501 ccgccatctg gttaaatgcg ataccaagtc gttcaaagct cgaaccctca tcaaagtgga
    13561 tacgttcatt gagatgccca tcgccaaatc gttgcgccgc tgcttccagt tttaacatat
    13621 cctgccagtg cggacgcatc cagataaaca ccggaaaggc gagggaaata gcaataaaag
    13681 cgatcagggc gatatccagc aatcgcattt gatggaggta ataaagataa ggaacaggac
    13741 caactgccag cacgtagtgg ctgcgcggga tacgctgcaa aaacgtgtac tgatcgtcca
    13801 gggcgacaat ttcgccgcca cgcagtcggt gcatggaaat atcatcaaga tggtatttac
    13861 tcagtggctc gacacgcaga tcgaaagaga gatttaaatc catctctttc agcgtcttac
    13921 cccagtcgtg tggggggatc tctcgcaatt cgctgcgcat cagatacagc gaactgttca
    13981 tcaaatcatc cagcgactgt ttgcccgcgc gttcggcggt aaatttgtac accagcccaa
    14041 ccagcagaga catcacaagg aagcagacaa acaataacag gtaaaactgg ataaacaatt
    14101 ttttcattgg catcgcccgc aacagaatac tattcgtatt aattcacgac tgtggagcaa
    14161 tgtaaaccgc caactggata agaccttgtt tggaagataa tgtccggaac agacggatgt
    14221 atccccggtg tattcatatt cagtaaattc aattttccac cggggcttgt ttattatgct
    14281 aaggtttaat ttaacgtgac gctttgttgt tctttagtct ctctatcctt aagaagacgg
    14341 gctgatatta caccggacat tattgaacca ttcacattaa gcgctgttct tgccatatca
    14401 acgatcggct ctatggcaat aaaaataact actaatgcga cagggaaatt aagtgttgaa
    14461 aggacaatca gcgccgcgaa tgtcccacca ctacctacgc ctgctacccc tattgagcca
    14521 agcgcgatgg caggtaaaag agttgcgagg aattgcaatg agaagggatc gatacccatt
    14581 gttggagcaa tcatcgccac catcacggca ggataaatcc cagcgcagcc attttgtccc
    14641 atgctggaac caaaagacgc tgcaatatta gcaatcgtat ttggaacccc caatttttcc
    14701 aatgcagaaa tagccagagg aattgaagcc gcactactgc gagaaacaaa cgcaaaggta
    14761 agtacttgcc agacggtctt gtaatagtta acggggtttt tacctattaa cagcaaaatg
    14821 cccgagtgaa caataaacat cacaaaaacg gccagataac aggcgacgat gaacccgagc
    14881 aaattggtaa actgtgaaag ctgatacgtt gagaagactt tcgccattag tgccattacc
    14941 ccataaggcg tcagcgcaat gacaatacgg accattttca ttacccatat ttgaatcgcg
    15001 ttgatccccg cgctcaattt ttcgccctct gctggtgcat ctttctttac ttttaacagc
    15061 gcaataccag caagaattgt aaagatgacg atgccaataa ccgaaacact tcttgccccg
    15121 gtcagatcaa ggaagaggtt ggttgggatc aatgacgtaa tgagtcgcgg taaagatacc
    15181 gcagctgttt tcgagacgtc atcgctggtt aaagaggatt gcatatgacc aaaggcgctg
    15241 gcatcaaggc caattgcact ggcaatagcc aggccaacta aacctgcaat catgactaaa
    15301 cacaacatac aggcaacaat agttaaagac atcttgccaa taccagcgct attttcgagc
    15361 ttattaatcg ctgaaagaat cgaaatgaat atgagtggaa cggcaacaag ttttaataaa
    15421 ttaacataac cgttaccagc aatattatac cattcgataa cggtttcact gcttgtctca
    15481 ggaatgaaat gcagggagat accaatgata gcccccagga aaatcgcgat aaaaacactt
    15541 ttggaaaggg attcgctctt ttgatccgtt ttgaaaatcc cccatgagca gatcaaaaaa
    15601 agcacaatat atgtgaaagt tgtaaccgtt ttttaagcct cttgcctcat tcagcacctg
    15661 aaatgccgga aagagtttgt tgtaaaaaat ctgcgccata agacgcaggg acgaggcgat
    15721 tatagagaaa ttttttctca ttttcatatc aaatgtaggg ggtttggaat ttgaataagc
    15781 tcttattaat tgaattcgtt aattgttctc tttttcagat gaacaaatat tagtcatctg
    15841 attgtttatt gtcactatcc catgcatgag gcgcaaaaag atagcctttg ttacgcacag
    15901 ttttaatgcg ataaggttct gcggcgttat cgagcagttt ttttcttaac cgcgaaatag
    15961 ccacgtccac gctacgatcc agtccgtcat aactgacgcc gcgtaaattt ttcagcaatg
    16021 catcgcggtc catgatttgc ccggcatgcg tagctagttc ccacaataat tcgaaatcag
    16081 ctgtcgagag cgagatttca gtgttagcca gggtgactac gcggttgatg gggtcgatag
    16141 ttaacgtgcc gaaatgcagg gctttgtagg gagtaagaga cgtttcctga agacctttgg
    16201 tcagtgtggc ttgctcattc tgacgcaaat gcaaacgtaa acgcgctagc aaaacagcag
    16261 ggggcgtcgt tttgagaata tagtcgcagg cacccatttc cagtgccagg atgtggttca
    16321 tatcgctatc gagagaggtt agaagaacaa tcggtccaga ccactttgcg cgtaaatcac
    16381 gacaaatggt catgccgtcc ttgcctggta gcatgatgtc gagtaacacc aaatccggat
    16441 tttctcgcaa aatggtttct tcggcctggt cgccgcgcgg ttctacggta acctgcatat
    16501 catgttttgc caggtacgcg gcaatcagtg aaccgacttc cgcatcatct tccacaaata
    16561 cgatagtgtt cataacattc acggtagata taaaaacgtc acaatacacc gcgctgtttt
    16621 tacttactat caatcgtttc taaacaagtt ttcatcaggt attctactga tgacttgtta
    16681 tggaagtgtt aaggtaaaaa gatggggctg gtaatcaaag ccgcgctcgg tgcgctggta
    16741 gtgctgttga ttggtgtttt agcaaaaacg aaaaattatt atatcgccgg gctgattcca
    16801 cttttcccga cctttgcgct tatcgcgcat tatattgttg ccagcgaacg cggcattgaa
    16861 gccttacgcg caaccatcat ttttagtatg tggtcgatta ttccctattt tgtttacctg
    16921 gtgtcgctgt ggtatttcac cggaatgatg cgcttgcccg ccgcttttgt tggttctgtt
    16981 gcgtgttggg ggataagcgc atgggtgttg attatatgct ggataaagct gcattaacgc
    17041 agatgacgac cgccatcaag tgggaaactg cgtccggtga caaagcaact ggtaagtaag
    17101 taatcgacca ggtcgatcac ttctttctcg ccaggcgcgg ttttcatcag tgatttattc
    17161 agcgcctgtt gtcgatattc ggcatcatca tgttcattaa acaggatcag cgatggcgca
    17221 atagaattca ctttcacttc cggtgccagc ttgcgggcaa acgagcgggt catattatcc
    17281 agtgccgctt tgcttgcagc atacgcaaca tgtttgtcgc taccgcgctc caccacataa
    17341 tcggtaaagt gaatgatatc gctggcggcg tgtccgtgcc cacgcagtaa tctttccagc
    17401 gcatggttga gcaggtatgg ggtattaacg tggatctgca tcatgcaagc cagtacgtcg
    17461 gccagtggcg cacccggttt ttccgcaatc cacgcactgg cgttatgcaa aatagcacgc
    17521 agaccatggg tggtttttag tacttcatcg gcaaacgcca tcacaccgtc gttggtcgaa
    17581 aaatcagcct gaatacactg cgcacctgca ttaatcagtc catcaatggc tggatagtgt
    17641 gtccgatagc tgacaatcac cggttgcttt tgattaatga aatgccatgc gagggcgagg
    17701 ccgatgcgac gacctccgcc agtaattaat attggcaagg gctgggtttt acccatcgtt
    17761 atctcctttg ctatccaacg atgggatgaa cgttatccca ccagcatcca ggtcgccgga
    17821 actgaggcaa ttaacagcat gccaatcagc accatttcct gacggttcag tacattatca
    17881 tgtgtatgcg ttttgcgcgc atacaggaaa accagcagcc ccggtgcata cagaacaacg
    17941 gataacagca agtgcattgg accagaggcg tacaataacc ataagccata aatgcaggca
    18001 ccgacaccta ccgctttatg cagtggacgg gttgcgattt tcagcaagaa cgcgccgacc
    18061 aggaaatagg gcaccagaat catttctgag gcgatggtca gcaacgtgtt gtaatcggaa
    18121 ccggtcagcc agatgagtac cagacaaatt tgcacgcaga tgttggtcaa ccacagtgag
    18181 gcggatggcg cagcttgtgc gttctggcgc gcaaaaatgc gtgggaatgc tttatgagtg
    18241 gcagccagga acggaacttc tgccgccatg atggtccagc ttaagtacgc gccgcaaacg
    18301 gaaacgatca aaccggcagc gatgatgatt tcgccccatg gtcccatcat ttccaccatc
    18361 agaccggcca ttgacgggtt acgaatttca gccagttcag gacgtgccac cacacccagc
    18421 gaaagcagcg ttaccagcaa gtaaacgccc agagcggaga gaaccgccag cagtgtcgct
    18481 ttgccaacat cacgtttatt acgcgcacgc gcagaaacaa ccacggcacc ttccacacca
    18541 atgaaaaccc acagggtgat cagcatggtg tttttcacct gttcccaaac aggtacgcca
    18601 agtgcaagtc cggtgaagtc gagcttgaag gtatccagtt tgaacatcat catcgccagc
    18661 acaacaaaca gacccagcgg caacaatttt gccagtgtcg ccaccaggtt aatgctggca
    18721 gcggtttgca ccccgcgcag gatcaaaaag tgaacaatcc acaataaagc cgatgcaccg
    18781 actatcgatt gccaggtatt accatcgcca aacaggcgca attccggcgt gtccgtaaaa
    18841 aagcttaacg cggaaaaaac gatcaccaga taggagacgt tggcgatgac cgcgcacagc
    18901 cagtatcccc atgcggaaca aaagccgatt agctcgccaa acccttcgcg ggcataggta
    18961 aagataccgc catcaagttc gggacgaatg cgcgtgagga tcagcatggc aaaggccagc
    19021 aataaaatgc cagcgccagt aataccccag ccgatgagca gtgctgccgg gctggcaact
    19081 gccgccatat tttgcggcag actgaaaaca cccgcgccca gcattgagct taataccagc
    19141 gcggtgagtg cgctcagtcc cagtttcttt tccatcgata tccagtgatt aaaacgtgat
    19201 aagaagaata aatattaggg caaaacgcat aaaaatggag tttgccactg aagcgcggga
    19261 ttttacgaag agatgtgtct gcatgcaatg gtcgcgggga aatttgtgac taaaaatgcg
    19321 aaaggcggca tcaagccgcc ttataggagt aactgaatag ttatttatac agatctgcgc
    19381 tgacggtcag gttattacca cgtttctgcc actggcgggt gatgtggtaa tacttagcac
    19441 ctttcttcgc ggcacgtttc gcaacctgat aggagacttc ggtcatgttg ccgtagttgc
    19501 cagagaattt gatgctgtcg aacgggacca tcatcgctgc tgtcgctttg ttcagttctt
    19561 cgactttagt gccatccggg agcgtgacgg tgtaacgccc gccttttgat gactgggttt
    19621 caaagaagcg accgacttca gaacttggtg atgcggtagt cgcaacaccc gggatctcaa
    19681 ctttcttcgc ggcttcgcca ccggcagcca gagctgcacg tcctgcttcg gaatctgccg
    19741 ggatcacatc cgggctctgg acgatacgtt tcttagcatc ttttttatag atgaatgcag
    19801 taatacgctg gttgccgccc tggttggcat cgatttgacg aacgatgtag aaagagtagg
    19861 cacctttcgc ttttgccgct ttggtgatgg catcattgac ttctggctgg ctacgataga
    19921 agccctggac ggtgaccgtg tcaaacggtt caatcagaac agcctgatct ttcggcagtt
    19981 cgacaacacc gttaattacg cgattacttg tttcttctgc tttttcagca tcggctttat
    20041 agaggtcagc gaccacacgc cagttaccgc tgttaccaaa atcagaagtg tcgacaacat
    20101 aaaaagaggc ggcaccttct ttatctgcgc gacgagaaac ggctttcacc gcttcgccaa
    20161 tagcattaaa acgaccggta accactacac ggtcaaaagg tttaaccgct gccgcttgct
    20221 ccggtgtcag ttctgttgct gcgttaacgg agaatgccgt agcagaaagc agtgccgacg
    20281 ccaggagggt gttcttaagc ttcataaaaa taatccttcg ccttgcgcaa accaggtact
    20341 ggtattgtta ttaacgagaa acgtggctga ttattgcatt taaacggtgt aactgtctgc
    20401 gtcatttttc atatcacatt ccttaaacca attttaatcc tgctcaaatg accgtctatg
    20461 cttaaaaaac agccgtatca gcatcattac tactgaagca actgaattgt ataagttaat
    20521 ttaatgttaa gtagtgattc gtgccggggc gatgtctcgt tttacccgac cgtcgaagac
    20581 aattatcagt ctttatccgg cgttctaagg tgtttatccc actatcacgg ctgaatcgtt
    20641 aatattttgc gagttcacgc cgaaatactg atttttggcg ctagatcaca ggcataattt
    20701 tcagtacgtt atagggcgtt tgttactaat ttattttaac ggagtaacat ttagctcgta
    20761 catgagcagc ttgtgtggct cctgacacag gcaaaccatc atcaataaaa ccgatggaag
    20821 ggaatatcat gcgaattggc ataccaagag aacggttaac caatgaaacc cgtgttgcag
    20881 caacgccaaa aacagtggaa cagctgctga aactgggttt taccgtcgcg gtagagagcg
    20941 gcgcgggtca actggcaagt tttgacgata aagcgtttgt gcaagcgggc gctgaaattg
    21001 tagaagggaa tagcgtctgg cagtcagaga tcattctgaa ggtcaatgcg ccgttagatg
    21061 atgaaattgc gttactgaat cccgggacaa cgctggtgag ttttatctgg cctgcgcaga
    21121 atccggaatt aatgcaaaaa cttgcggaac gtaacgtgac cgtgatggcg atggactctg
    21181 tgccgcgtat ctcacgcgca caatcgctgg acgcactaag ctcgatggcg aatatcgccg
    21241 gttatcgcgc cattgttgaa gcggcacatg aatttgggcg cttctttacc gggcaaatca
    21301 ctgcggccgg gaaagtgcca ccggcaaaag tgatggtgat tggtgccggt gttgcaggtt
    21361 tggccgccat tggcgcagca aacagtctcg gcgcgattgt gcgtgcattc gacacccgcc
    21421 cggaagtgaa agaacaagtt caaagtatgg gcgcggaatt cctcgagctg gattttaaag
    21481 aggaagcggg cagcggcgat ggctatgcca aagtgatgtc ggacgcgttc atcaaagcgg
    21541 aaatggaact ctttgccgcc caggcaaaag aggtcgatat cattgtcacc accgcgctta
    21601 ttccaggcaa accagcgccg aagctaatta cccgtgaaat ggttgactcc atgaaggcgg
    21661 gcagtgtgat tgtcgacctg gcagcccaaa acggtggcaa ctgtgaatac atcgtgccgg
    21721 gtgaaatctt cagtacggaa aatggtgtca aagtgattgg ttataccgat cttccgggcc
    21781 gtctgccgac gcaatcctca cagctttacg gtactaacct cgttaatctg ctgaaactgt
    21841 tgtgcaaaga gaaagacggc aatatcactg ttgattttga tgatgtggtg attcgcggcg
    21901 tgaccgtgat ccgtgcgggc gaaattacct ggccggcacc gccgattcag gtatcagctc
    21961 agccgcaggc ggcacaaaaa gcggcaccgg aagtgaaaac tgaggaaaaa tgtgcctgct
    22021 caccgtggcg taaatacgcg ttgatggcgc tggcaatcat cctgttcggt tggctgtcaa
    22081 gcgttgcgcc gaaagagttt cttgggcact tcaccgtttt cgcgctggcc tgcgttgtcg
    22141 gttattacgt ggtgtggaat gtatcgcacg cgctgcatac accgttgatg tcggttacca
    22201 acgcgatttc agggattatt gttgtcggag cactgttgca gattggccag ggaggctggg
    22261 ttagcttcct tagttttatc gcggtgctta tagccagcat taatattttc ggtggcttca
    22321 ccgtgactca gcgcatgctg aaaatgttcc gcaaaaatta aggggtaaca tatgtctgga
    22381 ggattagtta cagctgcata cattgttgcc gcgatcctgt ttatcttcag tctggccggt
    22441 ctttcgaaac atgaaacgtc tcgccagggt aacaacttcg gtatcgccgg gatggcgatt
    22501 gcgttaatcg caaccatttt tggaccggat acgggtaatg ttggctggat cttgctggcg
    22561 atggtcattg gtggggcaat tggtatccgg ctggcgaaga aagttgaaat gaccgaaatg
    22621 ccagaactgg tggcgatcct gcacagcttc gtgggtctgg cggcagtgct ggttggcttt
    22681 aacagttatc tgcatcatga cgcgggaatg gcaccgattc tggtcaatat tcacctgacg
    22741 gaagtgttcc tcggtatctt catcggggcg gtaacgttca cgggctcggt ggtggcgttc
    22801 ggcaaactgt gtggcaagat ttcgtctaaa ccgttgatgc tgccaaaccg tcacaaaatg
    22861 aacctggcgg ctctggtcgt ttccttcttg ctgctgattg tatttgttcg cacggacagc
    22921 gtcggcctgc aagtgctggc attgctgata atgaccgcca ttgcgctggt attcggctgg
    22981 catttagtcg cctccatcgg tggtgcagat atgccggtgg tggtgtcgat gctgaactcg
    23041 tactccggct gggcggctgc ggctgcgggc tttatgctca gcaacgacct gctgattgtg
    23101 accggtgcgc tggtcggttc ttcgggggct atcctttctt acattatgtg taaggcgatg
    23161 aaccgttcct ttatcagcgt tattgcgggt ggtttcggca ccgacggctc ttctactggc
    23221 gatgatcagg aagtgggtga gcaccgcgaa atcaccgcag aagagacagc ggaactgctg
    23281 aaaaactccc attcagtgat cattactccg gggtacggca tggcagtcgc gcaggcgcaa
    23341 tatcctgtcg ctgaaattac cgagaaactc cgcgctcgtg gtattaatgt gcgtttcggt
    23401 atccacccgg ttgcggggcg tttgcctgga catatgaacg tattgctggc tgaagcaaaa
    23461 gtaccgtatg acatcgtgct ggaaatggac gagatcaacg atgactttgc tgataccgat
    23521 accgtactgg tgattggtgc taacgatacg gttaacccgg cggcgcagga tgatccgaag
    23581 agtccgattg ctggtatgcc tgtgctggaa gtgtggaaag cgcagaacgt gattgtcttt
    23641 aaacgttcga tgaacactgg ctatgctggt gtgcaaaacc cgctgttctt caaggaaaac
    23701 acccacatgc tgtttggtga cgccaaagcc agcgtggatg caatcctgaa agctctgtaa
    23761 ccctgacggc ctctgcggag gccgtcactc tttattgaga tcgcttaaca gaacggcgat
    23821 gctttgacct cccgctgttt gttcaagcgc aattttgaca ataattgtca acggcacgga
    23881 aagcagcata cccaccggtc ctaacaacca tccccaaaaa atcaacgaca aaaataccac
    23941 caatgtcgaa agccccagcc cacgtcccat gatgcgcggc tccagaatat tgccgaagac
    24001 cagattaatc agcagatatc ccgccagcac cagcaacgct tcgtagaagc cattaaacac
    24061 cagtacctga gcgatagggg ggattgccgc gagaactgaa ccaatattag ggatgtaatt
    24121 aagcgcaaag gccagcaatc cccagacaaa agcgaagcga acatcgagtg cggcgagcat
    24181 cgcccaggcg accaggccgg tgatgatgct gatggctgtt ttcagcacca gataatgaga
    24241 aacactgtca atcgcacgtt gaatcgccgc catcccttca accggacgcg ccatcatttg
    24301 ctgaaatttt ccgggcaatt gtggcacttc gagcagcata aacagcaccg tcagcagcaa
    24361 taaaaatatt gatgacatgg cattagataa ctgcgtcaat aagttggtga gcaacgtcat
    24421 cgccgcgttc ggatcaatgt aatgcgccag ttggtcaact gagacgtcaa tccctacgcg
    24481 ttgcaacaac ggctcaagag cttgcagcgg cgtcataata gagttgcgat attgcggtaa
    24541 cgtccgcgtc aactcgttga gcgcggaacc cagataagct aatagcaaca ccatcgccat
    24601 cacgatgatt gtcatcaaaa tcgacactgc cagtacacgc ggcacacgcc agcggaccat
    24661 atgttgcacc agcgggttaa gaataacagc aataaataat gcgagaataa acggcacgat
    24721 gatctcggcg gcaaaacgga taccgcagag aataatgacc agcattccca acataatgac
    24781 gatttttagg ccattgagcg tgatgatcgg ctttgccatg cttgtccccg tttttcttcc
    24841 tttgctaata ataatgtctt tttcgctccg tcatcataac gaaaatcaaa ccgctcaggt
    24901 aaagaagtga aaatattttg agttaattct taagctatga tacaaatcag gcgtgttcaa
    24961 ctaccgagga caattatcat ctgcgatgac gagaagcaac actgcggata attgtaatat
    25021 tatggacaat ttgttcagga cgttattttc tacatttacc catcttcgta cctcatccac
    25081 tatcttgctc gtaggtgagc agcactggcg caacgcgctt tagtcctttc ttctgcctaa
    25141 gcttgcccta tgcgcagggc ttgaccactt tactttggtt tcgctgaatt aagcgaaaat
    25201 taaaatagtt ctcttgcagg agaaggacaa tgtatattta ttggatttta ttaggtctgg
    25261 ctattgctac agaaattacc ggtacgctgt caatgaaatg ggcgagcgtc agtgagggaa
    25321 atggcggctt tattttaatg ctggtgatga tttctctgtc gtatatattt ctctctttcg
    25381 ccgttaaaaa aatcgcctta ggcgtagctt atgcgctgtg ggaaggtatc ggtattttat
    25441 ttattacctt gtttagcgtt ttgttattcg acgaaagttt atcgctgatg aaaattgccg
    25501 ggttaaccac cctggtcgcc gggatagtgt tgatcaaatc aggtacccgt aaagcgcgta
    25561 aacctgaact ggaggtgaac catggcgcag tttgaatggg ttcacgccgc ctggctggca
    25621 ttggcaatcg tgctggaaat cgttgctaac gtctttttga aattttctga cggctttcgt
    25681 cgcaaaatat ttggcttgct ctccctggcg gcggtgctgg cggcctttag tgcgctttct
    25741 caggccgtta aagggatcga cttgtctgtc gtttatgcat tgtggggcgg gtttggtatt
    25801 gccgccacgt tagccgcagg ttggatcttg tttggtcaac ggttaaatcg taaaggctgg
    25861 attggcctgg tcttgctgtt ggctggaatg atcatggtga aacttgcctg atgaagacgc
    25921 tgcccgcgct gtcgggcagc gtttgaacat tatttttgcg acagttgatc cagcttgtcg
    25981 cgaaaaccgg taacagaaat ggcccggtta tcggcgcgcc agcgatcttt cgcggcagga
    26041 gccgaacttt gcaccccaat taattgccag ccgtcatcgg tatgcaacat cagaggcgaa
    26101 ccgctgtcac ccggcaaggt atcgcactga tgtgacatca ccgacgtttg cgcccagcca
    26161 gtcacttcac agttttgatg actgtataac gtatcgagat gatcttcagg gtatcccgcc
    26221 tgagtcactt tacgaccagc cgattttaat gcggcagtaa gcgcggcttt atctccctca
    26281 aataacggca acggtgtaat gccagaaggg ggattacgta gcacaatcaa tccgaagtcc
    26341 cacggtgcag ctgcgggagg aacaatccaa ccatccccat ctgcttttaa ccgctttccc
    26401 agtgtcggat caacgcggcc ttctatgtcg tggatctcat agcgccaaag acctttattt
    26461 gacacaaaac gcagcgctac cgccttatcc gctttaccct taggcggcgt caataaacag
    26521 tgtcctgccg ttaatgccag attgggtgca atcagcgtcg ccgtacataa attgccgctg
    26581 gccgtttcca gttgcccaac cgcatcccac ggtgattggg tcgtgtcatt cactggcaca
    26641 cgatcatcat gaccaaaaaa cagggtgctg acctcatcgt ttgccgattt ggcaacgtct
    26701 ggtttatctg caaacacaaa agcagacgtc aaactaattg cacccaacac tacagcaatg
    26761 gttgtacgca tatcacactc tggtgggtaa ttatgattat taaaagcaaa ccctcataaa
    26821 tactatagac gggacgggat gaaagtggga gtaaaatcag ataactacag tcaggaaaga
    26881 taaaaacggg cggcaatcag tgcgcataaa ataaggatga taagaatgaa ctcaaagcga
    26941 taacggccca ccatactacc ctccgctaaa ggcggtgcta aacgttaccc gcttagcacc
    27001 gtaattttca ggcgcgaggg ggcgcgccag cattactgtt gaaaacttac gctgcgggtt
    27061 gtgcagcagg ttttgccggt tgctgatggc cgtgtttccc ggcgtgtttc ttcgctgcct
    27121 gcgctttttg ttcaggggct ttctgttcag ctttcgtatt tttatgatgc tttttagccg
    27181 cctgcgcttt ctgggcaggt gctgctttat gctgtttttt atgatgtgta gttttcgctg
    27241 gcgctgcttt ggtggtcgtc gcagtcggag caggtgtggt cgcagtctct gcagcaaagg
    27301 cggcagaaga cagacccata gcagcggcaa caaccagagc taatactttt ttcattgtca
    27361 taccctcaat ttgttttttc atttaacccc actgcggggc cgttgaaata actatatccc
    27421 tgggaatggc agacttccgt gagtggttgg tttcagcgtg taacgatatg tacaaacgct
    27481 gaataaaata cagcgttgat aataacgtat tgtgacttaa ggaaaattta gctaccaata
    27541 atagtagtct tgatcgggtt aactattata ctacttacaa agcggtagaa taactgcgca
    27601 tcagtaataa atgacacaca gcaaaatgaa tccgtttatt tgggtactta taatcctgat
    27661 gacgctagat gcgctgcggg aattggctgg cgcttcgtct attttaggat ggctattaac
    27721 gttggtttga gcaggcaata agtccggacg ggtatttacc gcagtccgga cttatttttc
    27781 aggcgtgcag acgacgatgc aaacgcgtcc cgaccagcag agcaatgacc agcatcagag
    27841 caataaatgc gccgacgccg ttccagccat agttatgcca gaaaacacca cccagcgtcc
    27901 cggcaatact cgaccccaga tagtaactga acagatacag cgaggaggcc tggcctttag
    27961 cgcgttttgc gcgggggccg atccagctgc tggctactga gtgggctgcg aagaatccgg
    28021 ctgagaagag taacattccg gcaaagatca gccacagcga gctaaataag gtcatcagta
    28081 aaccaaacgg cataactccc gtcgaaaaca acatcaccgg accgcgccca tagcgggtgg
    28141 tcatggttcc ggctttgggt gagctccatg taccggtcaa ataagccagc gataataagc
    28201 caaccacggc ctgactgaca tgctaggggg aaagcatcaa ccgatagccg atgtaattaa
    28261 acagcgtgac gaacgacccc atcagcaaaa agccttctgc gaacaataac ggtaatcccc
    28321 ggtcacgcca gtgcagacga aagttgataa ataacgtctt agggcgcagc gaagtcgggc
    28381 aaaaatggcg tgattcaggg aggattttcc agaacatcaa cgccgaggcc agcgcgaaac
    28441 aaccgattgc cgccagagca attcgccagt tgaaaaagtc cgtgaagaca ccactaatta
    28501 agcgtccgct catgccgcca attgagttgc cgctgatata caaccccatc gaaaaggcca
    28561 cgaaactggg gtggatttcc tcgctaagat aagtcatgcc aacagctgcc acgccactta
    28621 acgaaagccc aatcaaggcg cgcataatca aaatgccgtg ccagctggtc atcattgtcg
    28681 aaagtaacgt acaaatggag gccaacagta gcgccgtgac catcactggt ttgcgaccaa
    28741 tggcatcgga tagcgggcca gtaaatagca aaccgatagc caacatcgcc gtggaaatgg
    28801 acagtgaaat actactgttc gcgggcgtta agccaaactc ctgcgaaagc accggaagga
    28861 taggctgcac acaatagaga agtgcaaagg ttgccagtcc ggcagagaac agcgccaggg
    28921 tgacgcgcat aaattgcggc gtaccgcgtt taataaattg atttggctga gaaatgcttt
    28981 gcttgtcagt gtcgcttgcc ggagcgccat caacagttgt agtacggctc acttgaaatc
    29041 cttgctaaat atgcctgtgg atcaggctta tacatagggt aggaaaatcg aattgttctg
    29101 tctaatatat taataatctc aaataagatg ttttaaatat gaatattgaa cttcgtcatc
    29161 tgcgttactt tgttgctgtt gcggaagagc tgcattttgg gcgcgcggct gcccgcctga
    29221 atatttcgca accgccgcta agtcagcaga ttcaggcact ggagcaacaa atcggtgccc
    29281 gactgctggc acgaactaat cgcagtgtat tgctgacggc agcaggaaaa cagtttcttg
    29341 cagatagtcg gcaaatcctg tctatggtgg atgacgctgc cgctcgcgct gaaagactgc
    29401 atcagggtga agcgggggag ttgcgtattg gttttacttc gtccgctcct tttattcggg
    29461 cggtgtccga tacgttatcg ctgtttcgcc gtgattatcc tgatgtccat ttacaaaccc
    29521 gcgaaatgaa cactcgcgag caaatcgctc cgctcattga aggaacgctg gatatgggat
    29581 tgctgcgtaa cacagcgtta ccggagacgc ttgaacacgc agtcatcgtc catgaaccgc
    29641 ttatggcgat gatcccgtac gatcatcccc tggcaaataa tccgagtgta acactggctg
    29701 aactggctga actggctgaa ctggcgaaag aaccctttgt cttttttgat ccgcacgtcg
    29761 ggactgggct gtatgacgat attctcggac tgatgcgacg ttaccatttg acgcccgtca
    29821 tcactcagga ggtgggcgag gcaatgacca tcatcggtct ggtttccgcc ggtctgggtg
    29881 tttcaatttt gcctgcgtca tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga
    29941 ttgctgaaga ggatgcggtt tctgaaatgt ggttggtctg gccgaaacat catgaacaaa
    30001 gtccagctgc gcgtaacttt cgtattcatc tgctgaatgc tctcaggtga gggaaatttc
    30061 agcgaaaaag cccgaaaaat gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac
    30121 gtattgaagt gcttcaccat agcctacaga ttatttcgga gcgcgaaaat atagggagta
    30181 tgcggtggtt gctgaaaacc agcctgggca cattgatcaa ataaagcaga ccaacgcggg
    30241 cgcggtttat cgcctgattg atcagcttgg tccagtctcg cgtatcgatc tttcccgtct
    30301 ggcgcagctg gctcctgcca gtatcactaa aattgtccgt gagatgctcg aagcacacct
    30361 ggtgcgagag ctggaaatca aagaagcggg gaaccgtggc cgtccggcgg tggggctggt
    30421 ggttgaaact gaagcctggc actatctttc tctgcgcatt agtcgcgggg agattttcct
    30481 tgctctgcgc gatctgagca gcaaactggt ggtggaagag tcgcaggaac tggcgttaaa
    30541 agatgacttg ccattgctgg atcgtatcat ttcccatatc gatcagtttt ttatccgcca
    30601 ccagaaaaaa cttgagcgtc taacttcgat tgccataacc ctgccgggaa ttattgatac
    30661 ggaaaatggt attgtacatc gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg
    30721 cgaggcgctg gagcagcata ccggcgtacc ggtttatatt cagcatgata tcagcgcatg
    30781 gacgatggca gaggccttgt ttggtgcctc acgcggggcg cgcgatgtga ttcaggtggt
    30841 tatcgatcac aacgtggggg tgggcgtcat taccgatggt catctgctac acgcaggcag
    30901 cagtagtctc gtggaaatag gccacacaca ggtcgaccca tatggtaaac gctgttattg
    30961 cgggaaccac ggctgcctcg aaaccatcgc cagtgtggac agtattcttg agctggcaca
    31021 gctgcgtctc aatcaatcca tgagctcgat gttacatggg caaccgttaa ccgtggactc
    31081 attgtgtcag gcggcattgc gcggcgatct actggcaaaa gacatcatta tcggggtggg
    31141 cgcgcatgta gggcgcattc ttgccatcat ggtgaattta tttaacccac aaaaaatact
    31201 gattggctca ccgttaagta aagcggcaga tatcctcttc ccggtcatct cagacagcat
    31261 ccgtcagcag gcccttcctg cgtatagtca gcacattagc gttgagagta ctcagttttc
    31321 taaccagggc acgatggcag gcgctgcact ggtaaaagac gcgatgtata acggttcttt
    31381 gttgattcgt ctgttgcagg gttaacattt tttaactgtt ctaccaaaat ttgcgctatc
    31441 tcaatttggg ccaggaaagc ataacttaga ctttcaaggt taattatttt cctagtttat
    31501 atttgtgaag cataacggtg gagttagtga tgctgaagcg tttctttatt accggtacag
    31561 acacttctgt agggaaaacg gtggtttccc gcgcattgct acaagcgtta gcctcccagg
    31621 gaaaaagggt tgcgggatat aaacccgtag cgaaggggag caaagagaca cccgaagggc
    31681 tgcgtaataa agatgccttg gtgttgcaga gtgtttcaac catcgaactg ccttatgaag
    31741 cagttaatcc tatcgcgtta agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca
    31801 attacaccct catttcaaac ggcctggcaa acctgaccga taaagtcgat catgtcgtgg
    31861 tagaagggac tggcggctgg cgcagtctga tgaatgattt gcgtccactc tctgaatggg
    31921 tagtgcagga acaactgccg gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc
    31981 atgcactgct aacagctcag gcgatcgcca acgacgggct gccgctcatt ggctgggtgg
    32041 ctaaccgaat caacccagga ctggcgcatt atgcggaaat cattgatgtg ctgggtaaaa
    32101 aacttccggc accgctcatt ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac
    32161 tggggcaata catccgctta gctatgctgc gcagtgtgct ggcggtagat agagtcacgg
    32221 tgtaacgtcc gcgaaattac cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc
    32281 tgatactccc cggtcatttc acatatcatc aacgtcgaca taatcggcgc gtgcgtggtt
    32341 gccgccaaca gtgtcgccat cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca
    32401 gggaaccata atcccaggct acgaccatac aacatgccaa tggcaagacc gataaatagc
    32461 gtcggtgtaa agacaccacc gggtgcgccg gaaccgctac tcgccagcac ggcacacagt
    32521 ttacaaagga agatcccggc aatgatcatt aacagtggtg gggcggttaa aaaggattgt
    32581 acggtgctat agccgttgcc ccacactgca ggtgtgaaca gggaaagcag acccacgatc
    32641 aacccgccta gtgccagttg ccagggcggc gcaagtttga gactcacaaa tccacgatga
    32701 caggcgttca ttaacgttaa caacagtggt ccgcacagac ctgccagcac acctgtactg
    32761 ataatcagcg catagtcacg agcctgaacc gtcactgaga gttgtacgtt gtagagtaac
    32821 gcgtcgctat gattaatcag attgctaacc agccatgcca cgatggcgga aataatcacc
    32881 gggccgagag aggccaacat catagtgcca aacagcactt cggctataaa taaactgcca
    32941 gcaagcgggg cacgataggc cgcagccatt cccgccgcgg ccccacaggc gatccataat
    33001 ttccattcct ggcgtggcgt aaaacgttgg gcaaaacagg aggcggcaag ggcagctaaa
    33061 agaatcatcg caccttcgcg accaattgca ctgccgctgg ttactaccag cagcgaggca
    33121 agcgatttaa ccaggcttgc tgcgtaatcg aactgtccat cggtttgcaa cgcttccatg
    33181 taatcggtcg gcgcatgagg gcgttgttgg gtaaatttct gccagcccat cagcaacaaa
    33241 cccgccgcca gtccgccgag cgccggagtt agcagccgtc gccaggagga aaggtttgtc
    33301 gctgcattga ccagactgcc ggagtcatta ttgaggaaca accactccag tagcagcatc
    33361 gcatgacgaa acccggcaac ggcaaagaca gcgagaatac cgacgactgt tgcgataagc
    33421 agacggcgga acatagtgcg cagatccgga tagatgtgta gatgatgcat gagcagaaat
    33481 gggcaatgaa acacgggcga ctattttgcg gcaaaagagg ccaaccgcaa aagcagtgcg
    33541 tgctttaaga gataaaaaaa cgtgacattg taacgtatta ttgccgaatc gtgcgtaatc
    33601 gtcaccatcc ggcaatatta cggtgatcct atcgaaagag tggtttaacc gcgacaggaa
    33661 tcaacagttg tgattgccat tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt
    33721 agaaagggtg ttctgctttt tcgataaaaa catcaagaaa acgtgttgac cacggaaaaa
    33781 ggtgccatgc cagcaattct tcgcattctg tctggcgacc attctccgcc aaccacgcag
    33841 ccatcaacag cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa
    33901 tgcctttctc gcgcatccac tgacgaagtg ccaatgttga atcgccaaac agcacagatt
    33961 cgcgatccag ccagaccgac ccccacggcg gagacggcag tgcccacggg ccgacaaaca
    34021 aacgctgcca ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg
    34081 gcgctaatga cgcttctggt agaggccact gagtttccca gccgtcactg gtgagtaccg
    34141 caaccagagg tgcggcttcc gcactctctg gagcgtaata aaacagcgcg cccaacacgc
    34201 gcgccgcgac agaaaaatta tcttgctgtg aaaaatgggt cattcctaac atcctgaaat
    34261 gtgcgggttg ccccgcacca atttgttaac ctgcaatagc cattcctact gtcatgtgca
    34321 ggccataaaa gagcacacgc ccgatcatct caccaacaag tatgaggatt agccccagaa
    34381 caagtccggc aacgtgaggt tcacggcgac gaatgagcgg acaaagccag catccaagac
    34441 ctgcgcataa caataccaca cgccagacct gtaaggaggc gtaatctggt accagtgcgc
    34501 tggcttgttg cacggatgag tgaatagacg ccagagacaa accttgcagc acaatcacct
    34561 cggcacaagc gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa
    34621 cgcgggctgc ccgcagaatt gcggcagcaa gtattggacc gctcaacaat accgtcagga
    34681 agaaagccag cgtagtgtaa ccgttatgcc aggttggcac ggtgtcgatt tgatacacgc
    34741 aggtcatcat ccagacgaaa atgacgccga gcgccatgct gacaagtaac cagagtttcc
    34801 ccaacgcttg cggcatttta ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa
    34861 aaaatatgga accggcagca atctcgttac tcagaccaga agctccgatt cgattcagcg
    34921 agttaaacgc tcgcagtggc gatcccaggt gcatgacaga ggcaataaac ccgatgccca
    34981 tcaacagcca gaggaaaaac atgccacgaa caatgtgctg acggtctgcg tcatttttgg
    35041 cggcgaacca gccaattccg ctaacaatca gcgcgcctac tacgcattga cccaatacgg
    35101 taaagataac cagtggccat tcatgccatc catttcccat tttacacttc ctccggatta
    35161 gccagataac cagtggtatc gccggtcggg cggctgttgg cgttaggttt gataacgata
    35221 ttgggttttg tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt gccgtgcttc
    35281 tggcgcagct cttcaattgg accgaactca agtgcacgca gcgggcagga ttcgacgcat
    35341 atgggttgtt tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt catgtgccct
    35401 ttttcagcat tgtactgtgg tgcgccgtac ggacaagcca tgtggcagta gcggcagccg
    35461 atacaaacat cttcatcaac caccacaaag ccatcttcac gcttatgcat tgcgacgctg
    35521 gggcagactt ttgtacatgc ggggtcatcg caatggttac aggagatgga gagataataa
    35581 gcgaacacgt tctggtgcca gatgccatta tcttcctgcc agtcgccgcc agcatattca
    35641 taaatacggc ggaaactgac ttccgggcca agatctttaa aatctttgca cgccagttcg
    35701 caggttttac agccagtaca gcggctggaa tcgataaaaa atccatattg tgtggtcatg
    35761 ggctactcct taaacctttt cgatctggac aagattgctg tgcgacgggt ttccctttgc
    35821 cagcggtgaa gggcggtgag aggtcagaat attgatactg ccgccgtgat cgacccggtc
    35881 accaaacata tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc caggcagaat
    35941 acgcggagtc acttttgcgg caatcagcat ttctccatta ttgttaaata cccgcacggt
    36001 atcgccatga cggataccgc gtgcctgagc atcaatgggg ttgatccaca cctcttgtgg
    36061 gcaggcctgc tgtaacacat caatattgcc gtagctggag tgggtacgcg ctttggtttg
    36121 gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga ggttgcgggt
    36181 tgggcgagga tcacatagcc catattccct gcagattcat gagagataag gtcttcttgc
    36241 tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc cgtcatgaag
    36301 tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc atgggtgtga
    36361 ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa aaacttgatg
    36421 gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat tttcgtacca
    36481 tggtcgatgg catctgtcca tgtaaaaacg gaaatctgcg ttttaacagg attctcgagc
    36541 atcgggaacc attctacccc cagatcccag ctaccttcgc gtacacctga gttgccgccg
    36601 tttatgccga cgttaccggt aagaacggaa agcatggcaa tagcgcggga cgtttgttcg
    36661 ccgttggagt gtcgttgtgg cccccaaccc tgacaaatat aagcaggttt tgctgaaccg
    36721 atctctcgtg ccaactggat aattttttct gccgggatgc tggtgatttt tgctgcccat
    36781 tccggcgttt tagctatgcc gtcaggccct tcgcccagaa tataggcttt ataatgcgcg
    36841 ttacgtggtg cgttggcggg cagcgttttt tcatcgtaac caacacaata tttgtcgaga
    36901 aatggctgat cgaccatgtt ttcagtaatc agtacccagg caatcgcaca ggccagtgcg
    36961 ccatcggtgc cagggcgaat gggcagccat tcatcttcac gcccggcagc tgtgtcgtta
    37021 tagcgtggat cgatgacgat catgcgtgcg tttgaacgtt cgcgggcttg ctcgacgtag
    37081 taagtgacac caccgccgct catccgcgtt tctgccgggt tatttccgaa cataacgacc
    37141 agtttcgtat tggcgatatc atccgggctg ttgccatcat tggcaccgaa catataactc
    37201 attgcggcac tgatctgtgc ggtactgtag ctgccatagc gactgagaaa acccccgcaa
    37261 gagttcatca gacggtacgg gacgttcgag ttggtgatat ttccgccatc tacgcctgtt
    37321 ccgtacagga catgtacagc ctcattgccg taatctttca ggatccgccg aagattatca
    37381 ctgatggtat ccagggcttc gtcccaactt atccgttcaa atttaccttc accgcgctcg
    37441 ccgacgcgct tcatgggata tttcaacctg tcaggatgat tcatccgtcg gcggatagag
    37501 cgcccgcgta aacatgcccg aacctgatga ttaccgtaaa cgtcgtcacc tgtcgtatca
    37561 gactccaccc agtacacggt gtcatctttc acatgcaaac gtagcagaca gcggctcccg
    37621 cagttaacgg tgcaggaact ccagaccgtt ttctcttcta ccggagcctc tgccgcccgg
    37681 accatttggg aaaatggcag agtgaaagca ctgcttgcca gcgcgagact gccaagtgcg
    37741 gaggttttca tcagacttct atggctgatt tcagccttca tgagcgcctc tgtggtatgg
    37801 attttcatca ttactcactt attgcttttc aaacaaaatg tcatgccaga atttatggtt
    37861 gtagtgggtt atattttttc gatctcgacc agattagtgt gctgcgggtt tcccttcgcc
    37921 agtggtgaag ggcgcagagt ggttagcgta ttcacacatc cgccatggtc gattttatcg
    37981 ccagacatat tgggctcgtg ccaggctccc tggcccatag cgctaactcc agggagaatg
    38041 cgtggtgtta ctttggctgg tagccgaact tcgccacgat ggttaaacac ccgcaccata
    38101 tcgccgttgg caatcccacg tttctgtgca tctatagggt tgatccacac ctcctgacgg
    38161 caggctgcct tcaggacatc aatgttgccg taggtcgagt gagtacggga tttgtaatgg
    38221 aaaccaaaca gttgcagtgg gaaggttcta cgttcagggg agtcccagcc ttcaaaggtt
    38281 gaggcataaa ctggcagtgg gcttatcact tcatcttttt ccagttccca ggtacgggca
    38341 atttccgcca gcttgctgga ataaatttca atcttaccgg aaggcgtttt aagtggattt
    38401 gcctcggggt cgtcacgaaa tgctttgtag gcgacaaaat ggccattggg atctttacgc
    38461 ttatagatac ccattttttt cagttcgtcg taagacggta acgccggatc tttggcaagc
    38521 attttggcgt acagatgttg taaccattgt tcctgcgtgc gaccttctgt gaacttttga
    38581 tagacgtcag gtccaagacg tttcgcgact tcactcagga tccagtaaat cggtttacgt
    38641 tcgaattttt cgctggtgac tggctggagg aaaatgagat atcccatgtt accggcgtag
    38701 tcgttaggaa taatatcttc ctgctcaacg gtcatcaggt cttccagcag aatgtcggca
    38761 tattttgccg atgaggtcat aaagttttcg atgaccacaa tcatttcgca tttcgattcg
    38821 tcctgcagaa tttcatgcgt tttgttgatg tcagaatgct gattaacgag ggtatttccc
    38881 gcgtagttcc agatggactc cattgccaga cggatggatg gacttgaagt agttgactca
    38941 atgctactta aggtcctgat atgactgaca ttaatcatga taacactgta tatccatcca
    39001 gttctttctc tgggtctccc tatcggtatc agcaagtcta cacctcaagc cctgacacaa
    39061 ggctgacaca aatcaatcta ctcagcgtag ctaagcctca gttagtccgc agagccaatg
    39121 gtcgatatac tatccgcttc agactcaagg gacagaccac accgtttctt tcagtatcga
    39181 cccgcagcac agacaggaga gtagccacaa tgcgtcagag agagcttgca gccaccgcta
    39241 aggccttcat gttggatagg cctgaagtct cactacagga gcttacagag catctccggt
    39301 ctatggctga gcagttcctg acagacgcta gtgacgatta ctggaatgga cttgaggtcg
    39361 ctactctggt cgacgagaag tccaacctga aggagctagc cgctacgcaa gccttgagtc
    39421 tggaccagca gaagggcata aggttagcgc tggaagtcct gaccgcagca cagcagagag
    39481 tcgacactgg tgatacctct ggtctgatta agctgattga tgataataac cttactgatg
    39541 actcaactat aggcgactct acgtcaattc tgaataatga gcagggggat agaccggcag
    39601 ttttcacaca ggagcgccag tcatctgtag ttttttcaag cctagtgtcc tcgctgctgg
    39661 ctgagaaggt ccagaccctg aagacatcca gctacaagga cctctcgtcc tcactcaata
    39721 ctgtctcccg gttcctgcct gaagacatgg acctgatgtc ccgctctggg tggctggctg
    39781 tcagggactc tatgctggct tctgaggtga gaccatcgac aatcaacaag ctgctgacca
    39841 aagcgaagat gtgtctcgat tatggcctga tgaatgggca acttgagggc cgcaacccta
    39901 ttgaacggat gaagctgacc aaggatatcg actccaagcg cagagcgttc acagacgagg
    39961 agctggagag acttctggtc cgtgttgagt ctg
//
LOCUS       DAJTRH010000027        38695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTRH010000027 DAJTRH010000000
VERSION     DAJTRH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO339_001557"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO339_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5926232.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="OO339_001558"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="OO339_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5926233.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="OO339_001559"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="OO339_001559"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5926234.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="OO339_001560"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="OO339_001560"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5926235.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="OO339_001561"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="OO339_001561"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5926236.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="OO339_001562"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="OO339_001562"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5926237.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="OO339_001563"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="OO339_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5926238.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="OO339_001564"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="OO339_001564"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5926239.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="OO339_001565"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="OO339_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5926240.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="OO339_001566"
     CDS             7139..7750
                     /locus_tag="OO339_001566"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5926241.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO339_001567"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO339_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5926242.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO339_001568"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO339_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO339_001569"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO339_001569"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5926243.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="OO339_001570"
     CDS             complement(11944..12303)
                     /locus_tag="OO339_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926244.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12300..12806)
                     /locus_tag="OO339_001571"
     CDS             complement(12300..12806)
                     /locus_tag="OO339_001571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926245.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12952..13515)
                     /locus_tag="OO339_001572"
     CDS             complement(12952..13515)
                     /locus_tag="OO339_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926246.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13722..14660)
                     /locus_tag="OO339_001573"
                     /pseudo
     CDS             complement(13722..14660)
                     /locus_tag="OO339_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14723..15097)
                     /locus_tag="OO339_001574"
     CDS             complement(14723..15097)
                     /locus_tag="OO339_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5926247.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="OO339_001575"
     CDS             complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="OO339_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5926248.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16269..17174)
                     /gene="rfbG"
                     /locus_tag="OO339_001576"
     CDS             complement(16269..17174)
                     /gene="rfbG"
                     /locus_tag="OO339_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5926249.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17167..18057)
                     /locus_tag="OO339_001577"
     CDS             complement(17167..18057)
                     /locus_tag="OO339_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5926250.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="OO339_001578"
     CDS             complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="OO339_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5926251.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="OO339_001579"
     CDS             complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="OO339_001579"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5926252.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="OO339_001580"
     CDS             complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="OO339_001580"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5926253.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="OO339_001581"
     CDS             complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="OO339_001581"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5926254.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="OO339_001582"
     CDS             complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="OO339_001582"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5926255.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23203..24096)
                     /gene="galF"
                     /locus_tag="OO339_001583"
     CDS             complement(23203..24096)
                     /gene="galF"
                     /locus_tag="OO339_001583"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5926256.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="OO339_001584"
     CDS             complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="OO339_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5926257.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="OO339_001585"
     CDS             complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="OO339_001585"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5926258.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="OO339_001586"
     CDS             complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="OO339_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5926259.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="OO339_001587"
     CDS             complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="OO339_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5926260.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="OO339_001588"
     CDS             complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="OO339_001588"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5926261.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31282..32652)
                     /gene="cpsG"
                     /locus_tag="OO339_001589"
                     /gene_synonym="manB"
     CDS             complement(31282..32652)
                     /gene="cpsG"
                     /locus_tag="OO339_001589"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5926262.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="OO339_001590"
     CDS             complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="OO339_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5926263.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="OO339_001591"
     CDS             complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="OO339_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5926264.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35504..35983)
                     /gene="gmm"
                     /locus_tag="OO339_001592"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35504..35983)
                     /gene="gmm"
                     /locus_tag="OO339_001592"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5926265.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35983..36951)
                     /gene="fcl"
                     /locus_tag="OO339_001593"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35983..36951)
                     /gene="fcl"
                     /locus_tag="OO339_001593"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5926266.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36954..38075)
                     /gene="gmd"
                     /locus_tag="OO339_001594"
     CDS             complement(36954..38075)
                     /gene="gmd"
                     /locus_tag="OO339_001594"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5926267.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38101..38649)
                     /gene="wcaF"
                     /locus_tag="OO339_001595"
     CDS             complement(38101..38649)
                     /gene="wcaF"
                     /locus_tag="OO339_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5926268.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
BASE COUNT        10334 a         9692 c         8706 g         9963 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    12541 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    12601 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    12661 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    12721 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    12781 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
    12841 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    12901 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    12961 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    13021 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    13081 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    13141 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    13201 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    13261 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    13321 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
    13381 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    13441 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    13501 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
    13561 taggcaataa aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat
    13621 gacaatacaa tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca
    13681 tacaaataca acggcaattc aatcatagta aaatactcta tttacattat cagtttatag
    13741 tttttttcag aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc
    13801 aaaataatgc cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag
    13861 gcagtcggaa atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct
    13921 aaaattatag acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt
    13981 tcaagcgagt aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat
    14041 agtttgctca cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc
    14101 atacgtaatt ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga
    14161 catggaaaag atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta
    14221 aaattccctt taaagatagt ctgaacagta ccagtcaaca atttatttcc tcagcgatgc
    14281 ccttttctgt atgtattttg atctccattc tcttgacgac atcctacaac catatccaat
    14341 tgatttttta tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct
    14401 ccatcaacca ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc
    14461 cccttgcctt tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct
    14521 gtactgtcag tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg
    14581 aaatcattta tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg
    14641 atattaaaag agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat
    14701 tgtgttttta acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta
    14761 aaaccaaact aaaaagagaa aaggtgaaca atgtaaatat tggagataaa gctaagagat
    14821 caaataaaac gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc
    14881 tagccttggt tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg
    14941 ctgcacatat aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac
    15001 acgcataaaa taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataactaa
    15061 aaaaagaact ggtcaataac ttccctattt ttaacatcct ttattttgct ccagaagtga
    15121 ggttattact aatttggata ttttctatag aaaataccca taaaactaca acgcctataa
    15181 taaaaattcc catgggtgta aaaagaaatg gattagttat agaaacggta atactgaaaa
    15241 aaagtattaa cgacatcatt aatgatgttt ttgtactatt taaaaaatag cctttcaaaa
    15301 agataagcaa cataggaact aatgatataa atacaccaat aacgcctgtt ttctgaagta
    15361 tctcaagagg tactatttca atccttaatc gatctaatat aaatgaacca aatcctcttc
    15421 caaacaagaa cgtcgctaaa tcaacatttt tataataaaa atataaatca ttaaatctaa
    15481 cagagtcaga atcttcactg cgtgatccga ccatgtaaag cataattata aatagaacgc
    15541 tgcccaatat tattataata gtatttaaat atagcttatg taatttaaaa ctggctaata
    15601 acagtgataa tgttgtaaaa agtaatacac ctcttgattc tgtcaataat atcgcaagca
    15661 ctattatgag acattttaaa tattttttgt cgacaaaact gataatcgca ccgacaccaa
    15721 aaaagtagaa ggatttggaa aaaaaagccc catcgggacg aaaaaagaac tcgctagtca
    15781 atgataagtg ttcataaatt aaattaaaat ttaacaaacc gaaattcaac aaaattatgt
    15841 atgaaatata taacagtgat ccatataatg caaatgcatt aactatttta ataaaaatct
    15901 cacatgaaaa tacttttaaa gtatatctta gtgtaaatag atatataagt gacagcataa
    15961 aaaggtatgg ttgcacatca aacagtatat catttatttt attaccagct aataagccga
    16021 caaagaagcc ataaaaagga aataaaagca accatataaa aagactgaca catttttggg
    16081 tgattgaaaa attgaatgta actaaattaa aaattaaaag tataaaacta aatacaaata
    16141 ataaatatcg caaagggaat attcccaatg gctccagtaa tcttccacca ccaccaataa
    16201 tcagttcaat acataaaaat gttataaaaa ttttatttat attattcata ttcgtaaggt
    16261 gatgtttttt aaaatatgtt taaaatcaac attaataaaa tataaatttt atcagccttt
    16321 ttccgcctta acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc
    16381 aattgattat tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt
    16441 cgataccact tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt
    16501 gctcccactt ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac
    16561 cacgaataat ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct
    16621 tgtgcagcat ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt
    16681 ataacatatg tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac
    16741 tgagccttat caactaaatt ttttcgccct gatggataat atgcaattac attgctggaa
    16801 taacaattcg cattattaaa taccaagcag tcaattgcat tttttatttt atctttatac
    16861 caaatatcgt cctgatcact aaaacagata taatcatagt tttcaagatc gacatcttta
    16921 attaatctat aaaaattttt acctgccccc ccaaacttat tgacgcttgg aagtatattt
    16981 ataattgagg gatatgataa ttgaaattct tcacaaatat ttaatgtatt atcagttgat
    17041 aaatcatctg atataaaaat tgagatgtct acatcttttt gttcaataat actttttaat
    17101 tgttcctcaa tccagcattc gccattatac gtagccataa gtacagctac tcttttttga
    17161 tcagaactat gcattataac gaccgccccc agtaattcct cttattccat ccagcgtagc
    17221 caaaatatgt tttttcatat attcaatttt atttttttga gttgcgataa ttaaagtcgt
    17281 atgaattaaa gaaaaaacaa cttccctaat agagagtaaa agaggcacat gattttttct
    17341 aagcaaaaga aatgcatttc ttactcgata ataacgacgt actggcgagt gaattggtat
    17401 gttaatccca cagattttta ctgacttatc cccaatagca tgaaccattt ctatatcagg
    17461 aatgatatga actaatatgc catagtttaa gcatctaaga caccattctg tatcgacata
    17521 atcaataaaa agttcctcat ccatcattcc aacaatctca aaaacttctt ttgaaaccat
    17581 ggttccggat gagattgtta ctgaactttt aaacggtgtc tgtccttcag tgacatgaat
    17641 tttctctcga agaccatttt ttttgatatt acaaatagga taataatgtg acttatctct
    17701 atcaataaaa actgggccag ctatttttgc attttcatta tccatatagc aggaaagttt
    17761 tttgataaac tcattgcgaa tacggctatc ttgatcaaaa aaaattatct tctctgcccc
    17821 tttttcaaga gaatatcgaa tcccaatatt ttgtgctttt gctatacctt cattcctacc
    17881 aagacaaatt aaatgaactt tttcaatgtc tgctaattta tctttcaagg tgaaattagt
    17941 cctattatcg actactacaa cgtaacaatt ttgctccttt agttcagtga tcagtgcatt
    18001 cagattttta agttcgggat tatatgtcac aatgacagcg taaatattac tattcattat
    18061 ccaagtgact cagtaattgg ttaattttag ttttcatata cagaacacat tctagtataa
    18121 agccattgta ataacagagc atctaaaatt acacgtatgc tccaggatat tgcggcaccc
    18181 agtaacccat atttttcgac cataaaatac aaaagggcta aataaggaac caattcagca
    18241 caatgaatca aagcagtaat tttagatttt cctaatgcct ggattgcaga gaatggtatc
    18301 attgccagcg cattgaagaa aaaaccgatc aataacacat ttaatatatt aaaggaatta
    18361 atttgataat tctcaccaaa ccatattttc aatacaagcc ctgaatataa caagcctatg
    18421 attatcaccg gcaaacagat tagaaacatt aaaagcaatg atttatttac attacgttta
    18481 aattctttaa agtctttgat gttacttaac cttggaaaca ctgccctccc gattgctgca
    18541 ggtattattc ctaattttag aataacctcc gctggtgcag aataaaatgc aactttatct
    18601 gcccccataa tatttgacac tataaatcga tcaaaataca ccatgaccgg acttatgata
    18661 ttacttactg tcatccaacc gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa
    18721 tgaacccccg aaattttaat atcattcctg acataatagg cagagactaa aatcgaaata
    18781 actcttgcaa atattaaacc agcaaccgct gccgacaatg tagcactata aaaaacaaat
    18841 atggccggaa ttcccgcgat gcaagagctt gatatagatt tttgaatatt tacaatgcca
    18901 aatttttcat ccccctcaag aatggctgac cataattgat ttagaataaa taatggaatg
    18961 caaatagcca acagtttaaa tgctagttgt atatcactat gttcaacacc agaaattttt
    19021 agataattaa caattccatc agagaaaatc aataataaaa aagcaccaaa gcatgaaaat
    19081 agcaccaaaa aagatgtact tgttgaaatt accttggttc tttcatgatg attatcacga
    19141 tgaattgcaa tttctctaat aacagagcga gtcaggccta catcaaaaat tccagcatac
    19201 ccaacaagtg caattgcaat tgtataaaca ccgaaacctt caggccccaa acctcgagcg
    19261 agaaatccta gcgaggggat agcaattaaa gttggtattg catagccaaa aaggttccag
    19321 acactatttt ttattatgct cattattaca tttctttatc atataaaata atcagcatct
    19381 acaaattttt gaccattaat atctttatca gaaagcaatg gattttgaac cggccattct
    19441 atatttatac ttttgtcgct ccataaaatc ccacgctctt gcatttttga atagaaatta
    19501 tttgttttgt agacaaattc tgcttcatca ctaagaacgc aaaacccatg agcgaatcct
    19561 tccggtatcc ataattgacg cttattctcc gccgacaaat ttacaccaac ccacttgcca
    19621 aatgttggtg atgatttccg aatatcaaca gctacgtcaa aaacttcacc gacaacacag
    19681 cgtaccagtt ttccttgagc ataaggttcc agctgatagt gcagtccgcg taacacaccc
    19741 ttagttgatt tagaatggtt atcctgaaca aactcaacct ttcgtcctac agcctcttcg
    19801 aaaactttct gattgaagct ttccataaaa aagccccgct catcaccaaa tacttttggt
    19861 tcaaaaatta gcacatcagg aatttcagtt ttaattacgt tcattttatt aataaccttt
    19921 aatcattttc agcagatact gaccataagc atttttcttc agcggctcgg ctaatacctt
    19981 cacttgctca gcatcaataa agcctttacg gtgagcaatt tcttccgggc aggaaacttt
    20041 cagcccctga cgctcttcaa ttgttgcaat gaagttgctt gcttcaataa gactttgatg
    20101 tgtccccgtg tccagccatg cataaccacg ccccatcata gcgacagaca aacgtccttg
    20161 gtccatataa atacggttaa tatcggtaat ttccagttcg ccacgtgcag atggtttaag
    20221 gtttttcgcc atttctacaa cgtcattgtc atagaaataa agcccagtaa ccgcatagtt
    20281 actttttggt tcctgcggtt tttcttccag actaattgcc gtaccgttat catcaaattc
    20341 caccacaccg tagcgctcag gatcatttac gtgataggca aaaaccgttg cgccactctc
    20401 tctgttaaca gcggtatcca ttaatttcgg caggtcgtga ccatagaaga tattatcacc
    20461 aagtaccagc gcacaatcat caccacctat aaattcttca ccaatgataa atgcttgcgc
    20521 cagaccatcc ggactcggtt gtactttata ttgcagatta agcccccact ggcttccatc
    20581 accaagcaat tgttgaaaac gtggtgtatc ctgcggcgta ctgataatca gaatatctcg
    20641 aatacccgcc aacataagcg ttgaaagcgg ataataaatc atcggtttat cataaatcgg
    20701 tagcagttgt ttgctcacag ccattgtcac aggataaaga cgagtgccgg aaccaccagc
    20761 cagaataata cctttacgcg ttttcatttc accattcctt ttaattcatc tcgctctagc
    20821 atcatgagcg agatgcaata atttgttaaa ttgccgtggt cgtaaataat tcgttgagca
    20881 tacgtttcac gcccacctgc cagtcaggca agacaagcgc aaagttctgc tgaaattttt
    20941 cggtattgag gcgagaatta tggggacgac gtgctggtgt gggataggcc gttgtcggca
    21001 cggcattaag tttgttaaga gcaaggttga tccccgctct gcgcgcttct tcaaatacca
    21061 gagcggcata atcgtgccag gttgttgtac caccagcaac cagatggtac aaaccagcaa
    21121 cttctggttt atttgcggcc acgcgaatgg catgagcggt gcaatcagcc agtaattcag
    21181 cccccgttgg tgcgccgaat tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca
    21241 ggcgcaacat cgttttggca aagttatttc ctttaccagc gtatacccag ctggtacgga
    21301 aaataagatg ctttgcgcaa tgttcttgta acgctttttc cccagctagc ttggtttcac
    21361 cgtagacatt tagcggtgct gttgcatccg tttccagcca tggcgtgtca ccgttgcctg
    21421 ggaaaacata atcagtggag tagtgtataa cccaggctcc gacttcatta gctgcttttg
    21481 caatcgcttc gacgcatgtc gcgttaagta attgcgcgaa attcggttct gattccgctt
    21541 tgtctactgc agtgtgagca gcagcattaa caataacatc ggggcgaatt tttttgacgg
    21601 tttcagccac accttcgggg ttgctgaaat ctccacaata atcagtggag tgaacatcga
    21661 gcgcaatcaa gttacccaga ggagccagag cacgctgcag ttcccaaccc acctgccctg
    21721 ttttgccgaa aagcaggata ttcattactg acggccctca tagttttgtt caatccacga
    21781 ctgataagta ccgcttttca cattctcaac ccaatttgta ttagccagat accattccac
    21841 cgttttacga atcccgctct caaacgtttc ttgcggtttc cagcccaatt cgcggctaat
    21901 tttatcggca tcaatggcgt aacggcggtc atgccccggg cgatcggcaa cataagtaat
    21961 ttgctcacga taagatttct ctttcggtac aatctcatcc aacaaatcac aaatagtgag
    22021 cactacgtcg atgtttttct tttcgttatg tccaccaatg ttataagttt cacccgcttt
    22081 accttcggtt acgacggtat ataacgcacg agcatgatcc tctacataca accagtcgcg
    22141 aatttgatcc cctttaccat aaataggtaa tgccttacct tccagagcat tcagaataac
    22201 caatggaata agtttttccg ggaagtgata aggaccataa ttgttcgagc aattagtcac
    22261 aatggttggt aaaccataag tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt
    22321 agacgcagaa tacgggctac ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc
    22381 ttcgttatta tttgcttcat ccggatgggg taagtcacca taaacttcat cagtagaaat
    22441 atgatgaaag cggaaacttt ttttcttttc atcattcaga gcagaccagt aattgcgcgc
    22501 cgcttctaaa agaacataag tccccacaat attggtttca ataaatgccg cagggcccgt
    22561 tattgaacgg tcaacgtggc tctctgccgc caggtgcatc accgcgtctg gctggtgctg
    22621 tgcgaaaata cgcgacatgg ctacagcatc gcagatatct gcatgctcaa aagcataacg
    22681 ttcagaatca gaaacatcag cgagtgattc caggttcccg gcgtatgtta atttatcgac
    22741 attaacaaca ctatcttgcg tattatttat tatgtgacga acaacagcag aaccaataaa
    22801 ccctgcgcca ccagtaacaa gtatcttcac ttttttattc catatagcta tagagcatgc
    22861 tgtgaaatag actgctccag atttgattaa tagaggtatt aatacacgct accgcccctg
    22921 gcttaacagc taccagtgca ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg
    22981 cgccaaatcc gaaaaattca aacgttaatt gtcttaccaa acagccgtag aaacaaggaa
    23041 aatcctggaa aaatttgaat aatatgagct atgtaacttc ttgtttttat aggctttata
    23101 aaaacggcaa cggcaggaaa attcaatgag caaaacttat gccacgaatc ttcactgccg
    23161 ttataatttt cttatcaacc gttacatccg gtcagatttt cattattcgc ttaacagctt
    23221 ctcaatacct ttacggaact tcgccccttc tttcaggttg cgtaggccat acttcacaaa
    23281 cgcctgcata tagcccattt ttttgccgca gtcgtaactg tcgccggtca tcagcattgc
    23341 atcaacggat tgttttttcg ccagctcggc aatagcatca gtcagctgaa tacgtcccca
    23401 tgcaccaggc tgagtacgtt ccagttccgg ccaaatatcg gcagaaagca catagcgacc
    23461 tacggccatg atgtctgagt ccagcgtctg cggctgatcc ggtttttcga taaattcaac
    23521 aatgcggctg actttaccct cacggtccag cggctcttta gtctggatga cggagtattc
    23581 agagaggtca cccggcatac gttttgccag cacctggctg cggcccgttt cgttgaaacg
    23641 tgcaatcatg gcagcaaggt tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac
    23701 gtctggcagt accacgacaa atgggttgtc accaatggca ggtcgcgcac acaaaatgga
    23761 gtggcctaaa cctaaaggtt cgccctgacg cacgttcata atgatcacgc ccggcggaca
    23821 gatggactgt acttccgcca gcagttgacg cttcacgcgc tgctcaagga gtgattctaa
    23881 ctcataagag gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag
    23941 gatttctttg atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggcttgtc
    24001 gacgattggt agcatctctt tgggtatcgc cttagtggca ggcaacatat gcatcccgag
    24061 acccgctaca ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt
    24121 tgctgaatta tagcttgttc gatttttttc gccagcatca attaccctga attgattact
    24181 gaattacttg tgatgttacg ccgcttcgtt gtggattgca gtagcattgt tcctaagtat
    24241 ggctccattt ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt
    24301 aatcttttcc acattaacaa tatggtgatt aatcctgtcg atatcgacgg agctttgccc
    24361 tctttcattc accgcatgaa catttgcaag agacagcagt gtttcttttt tcgccataaa
    24421 gacgccgcga acgtctttgc gcatgtcgaa gttcatgctc aatgctggtc caactgagga
    24481 ttcctgcatc acattgatat ttcgcataaa aagatgttgc ggcttgttgt gtaactccag
    24541 cgaggcacgc ttcatctcaa tgttagttag cgacacaaag gagacggcat tcccggcgga
    24601 gatttggatg ccgcgcaatt tataagcaag atgggtatta tccagttgaa tattattcac
    24661 tcggaaattt tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat
    24721 cccggcgcta ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc
    24781 gactgtcgcg ttatcaatgc ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt
    24841 gatattacga ataacaaaat gtttaccatt ttcaacatgg atcaactgcc gacaatccga
    24901 tcccgtgata ttcgccacga caaagttttt cactgcctgg tcttccgggt agttgttatc
    24961 ataagtgctt cccgcaaggc ctatgccgat gccccagttg attttgccgt tggtacagtt
    25021 gatacgctcg atgacatggt cagatatcaa aatatcactg tcgttaattg ccacgttcca
    25081 ttcaatggcg tcgccctgta agtcgctgaa cttacagttg gtaatgttgg caccgataat
    25141 ctggttatga aatccctggc gtaagatggc gtaattagcg tggctgacag tgaggttatc
    25201 gatggtcagg ttgcgcatga cccgtttgtt tttgccgcca atataaatct gcattaccgg
    25261 gccaaagccg ctcatcgcca gccctttgat ggtgcagtca gaaccacgca catccagggt
    25321 aatgttatgc ataccgccgc cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa
    25381 tcgccctctg ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc cgggagggat
    25441 gaagatgccg gtgttgatat tgtcacaaac gaatccggca ggcacgacga ccgtttgccc
    25501 ttcgctgaag gcttgtttaa atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt
    25561 aacgctttgt cgggcgggaa gtgcgcgagc gaaaggggta tggaggaagg caagcgccga
    25621 gcttgccgtc aggaaggtgc gtcgggagag tgttttaaat ggcatacgtt ctcctctata
    25681 aagcctgcag caagctggcg agttctcgat taatcacctg ctggttaaaa tcgtgttcga
    25741 ctttttcgca cgcacgtttg acgaccggag ccagttcgtc ggtatccagt tggctaaacg
    25801 ccgccagacg ttgtgccagt gcgcgagcat cgttctcagg cagcagccag ccggatttat
    25861 cggcctccac cagttcagga attccgctat gcagagtaga aaccaccgga ataccgaccg
    25921 ccatcgcttc cattagcgcc accggaatgc cttccatatc gccatccgcg cccgttaccg
    25981 atggcaacag gaagacatcc gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa
    26041 agcccggcat ctctaccaca tcttccagtt gatattgttc gatgatggtg cgcaggcgtc
    26101 gttcccacag gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact
    26161 gacggcaggc ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacagata
    26221 tgatttccag cggcgttgcg ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca
    26281 cgcccatacg cgatacggcg attttttctc tggggcagcc cattttttgc agccttccgg
    26341 cccacagatc gcttatcggt aacatcaggt cgccacggcg aaacagttgt tgatattcgg
    26401 gagtgtagtg gttgagcact tcccgactgg agatatcaat accgtggaag atggtggcaa
    26461 ttttgccgcg aatgataccc agttcgcgta gttttgctgc ggttaccccc gccgggccaa
    26521 aatgagcgat gaacacatcg gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca
    26581 aaatcaggtt ccgcgactcg gcaccatagc gtttgaggtt gagcgcatgc caggtatttt
    26641 tacgatgaat gccacgtaag gtctggctgg cgcggtggcg cagtttcgct actttgcccg
    26701 caggttcgtc ctgtaaccag cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt
    26761 gggtgttctc tgtatcgcct ttttgcaaag cgacaatctc cacttcaaag cccatatcaa
    26821 taaacgcggt aatttgattg aggacgaagg tttccgacga cagcgggaat ttcaataaga
    26881 agaagccgac cttcatttca cctccccgat gcgctcaagc acagactgca ccatctgcat
    26941 tcccgtctga cgttcgcgac tgacggcttc gttaaatcga gcgttcagca ccggaagctg
    27001 gcctaaggta tccgcaacca tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat
    27061 cgccatctct ggcagtccca gctgctgcat aatcccggcg gatttatgtt catagttgat
    27121 ggcaatcgcc ggagtagcaa agttcattga gataatggcg gagtgcaggc gcgtaccgac
    27181 ggtgagttca caggctccga gaattttgcc catttccaga tcgttgagtt catccatcac
    27241 tacgtggtaa cgggcaggat cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc
    27301 gtctttgtta tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc
    27361 atcgagaatg cgattgacca ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc
    27421 gagacgtttg tcgaacggtg ccagttcgcg cagggtaatt gccactgttt tctgttgtgc
    27481 ggcaacgtcc agccagtgtt gaacggcata gctggcggtg aagtcttcgg tgtggtgatc
    27541 gaccagccac gcggtatcga cgccatgttc cacttttgcg gtggtgatat ttctgcgttt
    27601 catcaaatca aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaagacata
    27661 gttcgccagt tggttaaatt gctcatcctg gaaggggccg acgctgtgac caatcataaa
    27721 cagcggcttt ttcgccataa atgtgcaaag cgcatgttca aactgcggca cgccgtagag
    27781 atcgacaaaa aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac
    27841 gaagtcggtg aatccctggg cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg
    27901 tgagagcaat acctgatgct ggtagcggcg acggaggact tttttaatgc gcccgacaac
    27961 gcccgccgcg ctattgtgtt gtgtcatctg caggaacagc ggatcgccca ttaccgggcg
    28021 gttgagcagc caggaagaac tgaccggata gcggctcatc acgtccactt cggtatgtgg
    28081 attgaggatg ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc
    28141 gcaagtgtgg ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt
    28201 gaggctgtgt ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta
    28261 tcaggcctac aggtcccgag cacagaaccg taggacggat aaggcgtttt acgccgcatc
    28321 cggcaaccgt tgtcggaacc gaaaacagca attcaccccg cccgtaaaag cattttcatt
    28381 ttttcgctgc gacaaaactg acgcttcact tccaccacca gcggatggcg tgacagcaca
    28441 atcatcacca caaacgccag cacgcccgcg gctatttgca ccgccagcag catccccagc
    28501 gccagttgcc ctttcagcac aatgcccagc gcataactga ccaccagcgt cggcaacgag
    28561 agataaaacg gcagccacaa actcaggatg tactggcgat aactggaacc aagaaccggt
    28621 ttaatcatca cgaagtaact cagaatggtg ttgataatct gcaccagcag gaaaccgagc
    28681 gtgacgccga ttgcgcccgc catctgccca ccaataacaa tcgccggaat aaacagaaat
    28741 gttttgaata cgttgaattt aaagctgata tcgacccgcg ctttcgccat cagcagcgaa
    28801 ccaatcgggt tacccacgga gcgcagcaga cccacaatac acagcaattg cagcaccgga
    28861 ataatgctgt tccacttttc gccgaatacc agcggtacga agttattcga caccaccatc
    28921 agccccagca gcgccggaaa gttgataatc cccactatcg acagcagctt gtagaagtta
    28981 acacgcagct tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg
    29041 atgattgggt tcagcttcat cggcggtacg acggccacgt tgtacgccag gttgtatccc
    29101 cctgccacgc ccgcgccaag aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga
    29161 tagttgatga tgctgtccgc cgtcagccag gcaccaaaac gtaagttcgg tgccacagac
    29221 gccagcgaga agtgcagacc ggggcaataa attttgcgtc caaagtagcc aaacagcagt
    29281 gttctcaccg cactattgac cagataaccg aggatcgcgg tcatcgccag cggccagaaa
    29341 tgggcgctaa ctaccgtaaa agtgaagccc gccagcaccg cgctggtttc gatcatgccg
    29401 attttgttga actccagctc cttctgcatc aacgcgcgga actgttgccc gtgggggatc
    29461 accacaaacg ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt attcagcacg
    29521 tcgccgatga catcactcaa caaaaacacc gccacacata ccacaagccc cagcccgacg
    29581 ttcagccagt acagcgtggt gagttcaagg tgactgattt cttttcgctg aataatcgag
    29641 ttagcgatac cgaagtcaga aagcgtatct gccagcgcga taatcaccag cgacacagtc
    29701 agcaggccga actggtggtt gtcgataatc cgcgccagca cggtcatctg caccagcccg
    29761 aggccgatga tgatcaccgt ggcaatagcc gaccacttcg cgccgctgat ggttttttca
    29821 cgtaagctca tatcaatatg ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac
    29881 gattttgata tcgaaccaga cgctccattc gcggatgtac tcaaggtcga actcgacgcg
    29941 tttttccatt ttctccagcg tgtcggtttc gccgcgccag ccattaatct gcgcccagcc
    30001 ggtaatgccc ggtttcactt tatggcgcag catgtagcct tcaatgagct ggcgatactg
    30061 ttcgttatgc gctactgcgt gcggacgtgg accgacaatc gacatccccc cggtcagcac
    30121 attgataaac tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc ccactttagt
    30181 gacgcgcgga tcgttctgcg tcgcctgggt caccactttg tcgttctcca tcactttcat
    30241 ggaacggaac ttccacactt tgatcggctt gccatccata ccatagcgag tctggcggaa
    30301 aataaccggc cctggtgaac tgagtttcac cgccagcgca atacagcaca gcaccgggga
    30361 gatcagcagc agaataagcg tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt
    30421 aacccccgaa agcggcgtgt catacagcgg caccaccgga acgccgttca tctcttcgag
    30481 acgtgaatgg agaatgttga aggtaaagac gtcggggatc agcagcaccg aacaggtggt
    30541 atccgccagt tgatggacca gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat
    30601 atagacgtta tgaatcttgc ccgcttttgc atcctcgacc agctgttgca ggttacccgc
    30661 ccagtcgtta gaaacgccgc ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc
    30721 taaccacggc tgattacgga aactctccat cagcatttgt ccggcggcta aatcccccgc
    30781 tacggcgacc atccgcttgt tgtagccatg attacgcagc cagcccgccc caatgcgaat
    30841 acatgaacgg caaaccacca gtccgatgct ggtcagccca taccacgcca gccagatttt
    30901 cagttgcgtg tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa
    30961 ggtccagttt tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg
    31021 ataaaaatcg gtgatgccgc ccagcatctg gaacaccacc agcgtaatca gcgccaccaa
    31081 caggtgcatg tagaggaatg acagtccgct gacttcgcaa accagccata gcccggcaaa
    31141 catgatggtg atatctgaaa agcgttgcac catagagatt aacgatgcat tggttttcgc
    31201 tcgctcgcgc ttttttagat ttgtcatcgt tgttcctgtt attagcccct tacccgcagt
    31261 ggggtaaggg aagatccgac attactcgtt cagcaacgtc agcagagttc gcgttcgcgc
    31321 ttccatcagc ggcacatcac cgcgcgattc cacattcagg cgcaccaccg gttcggtatt
    31381 ggaggtgcgc aggttaaagc gccagtcggc aaaggtcatg ctgatgccat cgctgcgatc
    31441 caccgccagc gcctcacggc taaaatgctg ttccacgcgg ttaatcgcct caacgggttg
    31501 cgccagtttg ctgttgatct caccgcttgc cggaaacgcc gccatccggt cgcgcaccag
    31561 ttcgcccagc gtttttcctt tcaggcacac cagttcggcg accagcagcc acgggatcat
    31621 gccgctgtcg cagtaagcga aatcacggaa gtaatggtgg gcgctcattt cgccaccgta
    31681 gatggcgtct tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacattac
    31741 cggcgtgccg cctgcagcag tcaccacatc aatagtgttc caagagagac gcggatcatg
    31801 gatgatcttc gcgccgggat ttttttcgag gaacgcttct gccagcaggc cgacaatgta
    31861 gtagccctcg ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc
    31921 aaaggcaatg cccatatccg cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca
    31981 ttccggcagc agcgggttag gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac
    32041 tttgattaat tccacgggcg cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac
    32101 cggacccgct gcgccattcc cggagttgat caccagcttg agcggcgtga gatttttgac
    32161 attgatataa ccgaacaggt gatcaacgta agcgtcacgc aggttgattt gctgatagcg
    32221 accgcgtttg gtttcatcga cgggaggaaa gtcgttggct tcagccagac gctggacgtc
    32281 gcgcagtccg gtatcaccgc tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt
    32341 gtaatccatc ggattatggc tggcagtaac ttcaatgccg ccatccacgc cgagatggaa
    32401 cgtggcgaaa tagatctctt cggtgccgga cataccgata tccagcacat cgacgcccgc
    32461 atcctgtaaa ccttttgcca gcgccagttt taaggtttcg ctggtgagac ggacatcacc
    32521 gcctaacaca atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc
    32581 gatatcttca ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt
    32641 taattttttc atgtcgttac cctttttcag gcaatagttg gccctgaccg aaacggccaa
    32701 ttttattttt gtcgttattt acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc
    32761 gcgtcttatc aggcctacaa aaccgaaccg taggtcggat aaggcgttca cgccgcatcc
    32821 gacaaacagc gcctgatgcg acgtttacac ccgtccgtag cgatccgcga agcgcaccac
    32881 atcatcctct tcgagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc
    32941 cgggttttcc aggcagtgcg tcgcccccag cggaatataa atggactcgt tttcaccaag
    33001 cagtttgata tcaccatcaa tggtgacttt tgccgttccc gcgaccacta cccagtgttc
    33061 cgcgcggtga tggtgcatct gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt
    33121 cacctggtag cggtcgcccg cgtcgataga gtcatatttg ccccacggac gatacacttc
    33181 gcgatgcacc cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac
    33241 atcctgtacc gcgttacggt cggcaatcag caccgcatct ttggtctgca ccactaccag
    33301 atctttcacc ccgacggtgg tgaccaggcc tgattcggca tacacatagc tgttttcagt
    33361 tttgtgatta atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc
    33421 ccataatgaa gaccaggaac caacatcgct ccagcccgca tccatcggca ccacaacagc
    33481 atctgccgta ggttccatga ccgcgtaatc caccgactct tccgggcagg cgagaaacgc
    33541 ttcttcatcc acacgaataa aatcgagatc cggatcgacg gcgctcatcg ctttttcgca
    33601 ggcatcgagg atatccgggc gatatttttt cagttcttcg agatagcgtc cggcgcggaa
    33661 caggaacatg ccgctgttcc agtaatattc gccgcttgct acataggcct gggcggtttc
    33721 cagattcggt ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccac
    33781 agacacttca ccgcgacgaa tatagccata accggtttct ggtagatccg gcacaatgcc
    33841 aaaggtcacc agcttgcccg cttcggcata aggcatggca ttacgcacgg cggcacggaa
    33901 cgcgtcttca tcggcaatca catgatccgc cgccaatacc agcattaacg ggtcgctctc
    33961 cgggctatga cgttttgccg ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc
    34021 tgccggttcg agaataatgt tctcggtgag tttgttcagt tgacgcagct gttccgcgac
    34081 aataaagcgg tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag
    34141 gcggcagatg gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg
    34201 gtaaagtacg cgggaaagcg gccataaacg gctaccggag ccacctgcca tcacaactgg
    34261 atagagtttc gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg
    34321 ttctctttat cgagcgtgcg ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc
    34381 aggagcgcct gacgaatccc cgccaccagc gcctcgaccg attccggttc tacgcaaacc
    34441 gcaatacccg gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc
    34501 accgcgttac cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc
    34561 gcgccgcgtt tttgcaccac cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg
    34621 tcatacgatt gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt
    34681 tccagccgcg ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc
    34741 aggagatcgg cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag
    34801 taaagaatga tttttttgtt atccggcagg ccaagctggt tacgaagggc atcaacatcg
    34861 gcgtctgcaa catgctgaaa acgggcaatt tccgaccagt tggggaagaa gatgacgtta
    34921 tccgccgcca cgcctttttc gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg
    34981 ttatcgacgt tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc cactttgccg
    35041 cctttgcctt ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata
    35101 tgcagcacgg tacgcgcacc agagagcttc gccagcaggc gcattcccgg cgtgcaaaac
    35161 agcgttggca ctacgccaat aatgcgatcc ggcttccagc gacgttgcgc catcagtgga
    35221 aagaaactgc tgacggcaaa actgccgaga tgcaacaagc gtttcagggt gctcggctgt
    35281 tttggcacgt acagcgggca gcgccacacc gtggcggccc cttcttctcg tttgtagcgc
    35341 caggcggaat agttctcacc cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc
    35401 cgcacctcat gaccttgtgc cgccaaccat tccaccatct cgccggtgta tttgccgatg
    35461 ccggttaact ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc
    35521 cggcacgctt ctcagcgaga aaataggctc ggctgttagc gtgaacatcg ttactggcga
    35581 gcagcgcgtc cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtaacagct
    35641 cttcttccgc tactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc
    35701 cggagaagtt atcgtcataa aaatgctgcc agacaccgta aaactggcct gctgttatcg
    35761 gcagacgcag ccccagttcc gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt
    35821 ctttctgcac gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt
    35881 tgccaagcag aaactcgccg cgactgttct cgacaataaa gtcgagagag acaagcggtg
    35941 tggagcgcac tacagtggca aagtcttcct gacgtaaaaa catcattccc cccgaaagcg
    36001 gtcttgattc tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc
    36061 gtgataccag ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc
    36121 cggtttgctg gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg
    36181 cgccagctcg cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg
    36241 ttgggtattc tccagccaga cttcatgcgc cagctccatg acatgaatgc tcgccgccgc
    36301 catatcatcg acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc
    36361 cggcgcattc tgtgccgtcg cctcgtggaa gcgacgcagc aatgctggta tcacatggga
    36421 gttactcggg tggaagttgt catgcggccc gtacaggttg gtcggcatga cagtgcggta
    36481 atcgcgtcca tactggcggt tgtaagattc gcacagtttg atcccggcga ttttggcaat
    36541 ggcgtaaggc tcgttggtcg gctccagcgt gccctgcaat aactcgcttt ctgccatcgg
    36601 ctgttttgcc agtttcgggt agatgcagga cgatccgaga aacatcagtt tgttcacgtc
    36661 gttctgatgc gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc
    36721 cgccggatag gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg
    36781 gtcaatgcgt tcgctggcaa agaaatcatg cacggcgcgg ctgtccagca ggttcagttc
    36841 atcgcgggta cgtaatacca gctccacatc accgcgctgt tcgagctgcc gcttgatggc
    36901 agaaccgacc atcccacaat ggccagcgat aaaaatgcgt tgtttactca tgcttatgac
    36961 tccagcgcga tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct
    37021 tcgaggtcat tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag
    37081 cccagttttt cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc tgccggacgg
    37141 aagtaacgcg gatcaacggc gataatcaca tcacccggtt taacgcccgg cgcgtcatgc
    37201 ccggtgacgg aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg
    37261 atgcccagct gtgctgccgc catttccaca aactgacgta cggagtactg aacgccggtc
    37321 gcgataacga aatcttccgg ctgttcctgt tgcagcatca tccactgcat ttttacgtag
    37381 tctttggcgt ggccccagtc acgcagggaa tccatattgc cgaggtacag gcacgactcc
    37441 agcccctggg cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg
    37501 cggcgcgggg attcatggtt gaagagaatt ccgttacaag cgtacatgcc gtaggattcg
    37561 cggtagttaa cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agatcgcggg
    37621 tagaacggcg tggtttcttt ctgcggaatt tcctgcacca gaccatacag ttcagaggtg
    37681 gaagcctgat agaaacgagt tttcttttcc agaccgagga agcggatcgc ctccagcagg
    37741 cgcagcgtac ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa agagaccgca
    37801 acgtggctca ttgcgcctag gttgtacact tcatccggct gtacttcacg caaaatgcgc
    37861 gtcaggttag aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg
    37921 tgcggatcct gataaatgtg atccacgcgc tcggtgttga atgacgatgc gcgacgctta
    37981 ataccatgca cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt
    38041 ccggttacac cggtgatgag agcgactttt gacatgtctt attcctctgt atttttgaat
    38101 ttattcagtt tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc aaaccacatt
    38161 tgccggaagc gatttaaaaa cactgcttcg tgcacccacg acggtgccgt cgccgattgt
    38221 gacgcctggg gcaacaaaga catcggttgc cagccagcat ttctcgccaa tcacaatagg
    38281 cgtggcgtta atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc ataaataact
    38341 tttttgcgat atcaccgaat gtgcgccaat ggttatttca ccgagggtat ataaattgac
    38401 gtcatcgcca acccacgcgt aatcacctaa ggttaatttc cacggatagg taatttttac
    38461 tgacggacga ataactacgt tttttcctat ttttgctccg aataaacgta ataaaaaagc
    38521 ccgccagcga tacaatactt gtggcgacca ggcaaatatt gttgcctgta ctgcccacca
    38581 taattgcact ttaatagcgt tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa
    38641 atcttgcatt ggttttcctt atattcagac tttgttatat aaggctttag tctta
//
LOCUS       DAJTRH010000028        37294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTRH010000028 DAJTRH010000000
VERSION     DAJTRH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..175)
                     /locus_tag="OO339_001596"
     CDS             complement(<1..175)
                     /locus_tag="OO339_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5926269.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO339_001597"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO339_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5926270.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO339_001598"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO339_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5926271.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO339_001599"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO339_001599"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5926272.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO339_001600"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO339_001600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5926273.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO339_001601"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO339_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5926274.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="OO339_001602"
     CDS             complement(5179..5421)
                     /locus_tag="OO339_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5926275.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO339_001603"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO339_001603"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5926276.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO339_001604"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO339_001604"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5926277.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="OO339_001605"
     CDS             complement(8338..9312)
                     /locus_tag="OO339_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5926278.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="OO339_001606"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="OO339_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5926279.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="OO339_001607"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="OO339_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5926280.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="OO339_001608"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="OO339_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5926281.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="OO339_001609"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="OO339_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5926282.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="OO339_001610"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="OO339_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5926283.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="OO339_001611"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="OO339_001611"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5926284.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="OO339_001612"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="OO339_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5926285.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="OO339_001613"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="OO339_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5926286.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO339_001614"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO339_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5926287.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="OO339_001615"
     CDS             23179..23382
                     /locus_tag="OO339_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5926288.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="OO339_001616"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="OO339_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5926289.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="OO339_001617"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="OO339_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5926290.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="OO339_001618"
     CDS             26387..26659
                     /locus_tag="OO339_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5926291.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO339_001619"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO339_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5926292.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO339_001620"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO339_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5926293.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OO339_001621"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OO339_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5926294.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO339_001622"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO339_001622"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5926295.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO339_001623"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO339_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="OO339_001624"
     CDS             complement(31906..32304)
                     /locus_tag="OO339_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5926296.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /gene="zapE"
                     /locus_tag="OO339_001625"
     CDS             32498..33625
                     /gene="zapE"
                     /locus_tag="OO339_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5926297.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33844..34272
                     /gene="rplM"
                     /locus_tag="OO339_001626"
     CDS             33844..34272
                     /gene="rplM"
                     /locus_tag="OO339_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5926298.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34288..34680
                     /gene="rpsI"
                     /locus_tag="OO339_001627"
     CDS             34288..34680
                     /gene="rpsI"
                     /locus_tag="OO339_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5926299.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35075..35713
                     /gene="sspA"
                     /locus_tag="OO339_001628"
     CDS             35075..35713
                     /gene="sspA"
                     /locus_tag="OO339_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5926300.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35719..36216
                     /gene="sspB"
                     /locus_tag="OO339_001629"
     CDS             35719..36216
                     /gene="sspB"
                     /locus_tag="OO339_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5926301.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36259..>37294)
                     /gene="dcuC"
                     /locus_tag="OO339_001630"
     CDS             complement(36259..>37294)
                     /gene="dcuC"
                     /locus_tag="OO339_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5926302.1"
                     /translation="SPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVS
                     RLSAVAVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHF
                     FVQRAFDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSEL
                     HLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKG
                     LTTIGTVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAA
                     GLHVPAVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVV
                     NMIATITLFY"
BASE COUNT         9024 a         9319 c         9824 g         9127 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaactggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatctgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagagtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgggcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctcac cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac tggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg acaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acggcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttcccaaaac
    33721 tttttgtgtg ctggcatagg ctattcgaag gggtaggttt gccggacttt gtcgtgtgaa
    33781 cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta actatttatt gggtaagctt
    33841 ttaatgaaaa cttttacagc taaaccagaa accgtaaaac gcgactggta tgttgttgac
    33901 gcgaccggta aaactctggg ccgtctggct actgaactgg ctcgtcgcct gcgcggtaag
    33961 cacaaagcgg aatatactcc gcacgtagat accggtgatt acatcatcgt tctgaacgct
    34021 gacaaagttg ctgtaaccgg caacaagcgt actgacaaag tgtactatca ccacaccggc
    34081 cacatcggtg gtatcaaaca agcgaccttt gaagagatga ttgctcgccg tcctgagcgt
    34141 gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc cgctgggtcg tgctatgttc
    34201 cgtaaactga aagtttacgc gggtaacgag cacaaccacg cggcacagca accgcaagtt
    34261 cttgacatct aatcgggatt ataggcaatg gctgaaaatc aatactacgg cactggtcgc
    34321 cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca acggtaaaat cgtaatcaac
    34381 caacgttctc tggaacagta cttcggtcgt gaaactgccc gcatggtagt tcgtcagccg
    34441 ctggaactgg tcgacatggt tgagaaactg gacctgtaca tcaccgttaa aggtggtggt
    34501 atctctggtc aggctggtgc gatccgtcac ggtatcaccc gcgctctgat ggaatacgac
    34561 gagtccctgc gttctgaact gcgtaaagct ggcttcgtta ctcgtgacgc tcgtcaggtt
    34621 gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc cgcagttctc caaacgttaa
    34681 ttggcttctg ctacggcaga aaacaatttg cgaaaaaacc cgcttcggcg ggttttttta
    34741 tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct ttttgctgcc gtctgaaaga
    34801 caggtaaaac aaaaaccaca tcgccaataa gggtctaagt caactatttc agactaaagc
    34861 gcatctcttt ttccccattt ccggcatcca ctcaccacaa aggtcgcaaa atctggtaaa
    34921 ctatcatcca attttctgcc caaatgtcgg gtattgctca ttttttgttt gattttcgaa
    34981 caaagagagt cgttccttat tgggtaacac aacttctgac tggccacctg gtggctggta
    35041 gcagtaaaaa ttctgactat acctggaggt tttcatggct gtcgctgcca acaaacgttc
    35101 ggtaatgacg ctgttttccg gtcctactga catctatagc catcaggtcc gcattgtgct
    35161 ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa aaggacaatc cgcctcagga
    35221 tctgattgac ctcaacccga atcagagcgt tccgaccctg gtggatcgtg agctgaccct
    35281 gtgggaatct cgcatcatta tggaatatct ggatgagcgt ttcccgcatc cgccactgat
    35341 gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac atgcatcgca tcgaaaaaga
    35401 ctggtacacg ctgatgaaca ctatcatcaa cggttcagct tctgaagcag atgccgcacg
    35461 taagcaactg cgcgaagaac tgctggcgat tgcgccggtc ttcggtcaga agccgtactt
    35521 cctgagcgat gagttcagcc tggtcgattg ctaccttgct ccgctgctgt ggcgtctgcc
    35581 gcaactgggc atcgagttca gcggcccggg tgcgaaagag ctgaaaggct atatgacccg
    35641 cgtctttgag cgtgactctt tccttgcttc tttaactgaa gcagaacgtg aaatgcgtct
    35701 gggccggagt taatctgtat ggatttgtca cagctaacac cacgtcgtcc ctatctgctg
    35761 cgtgcattct atgagtggtt gctggataac cagctcacgc cgcacctggt ggtggatgtg
    35821 acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac
    35881 attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg atgaggtgcg ctttaacgcg
    35941 cgctttggtg gcattccgcg tcaggtttct gtgccgctgg ctgccgtgct ggctatctac
    36001 gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag ctgcctacga tgaagatacc
    36061 agcatcatga atgatgaaga ggcatcggca gacaacgaaa ccgttatgtc ggttattgat
    36121 ggcgacaagc cagatcacga tgatgacact catcctgacg atgaacctcc gcagccacca
    36181 cgcggtggtc gaccggcatt acgcgttgtg aagtaataca aaacaggccc aggcggcctg
    36241 ttttgtcttt ttaatgactt aataaaatag cgtgattgtg gcaatcatat tcaccacgaa
    36301 accgactgcc atggggatcg ctgtccgctt caccaccgca aaaggcgaaa cgcctgcaat
    36361 tcctgacgta acgaccacca ccgcagtaat cggcgaaacc gcgcgcgcta gcgtcgtggc
    36421 aaaatgcatc ggcataatca ttacaaccgc tggtacatgt agtccggctg cgatattcgg
    36481 aataagactg gcaaatgaca taaacggcgc attgccagag cccatcacaa tggcacaaat
    36541 ggcaatgact agcgccataa taatcatcac gccaataccg cccagaccag aatgctccgc
    36601 acccctgata accgcatcaa cagtgccaat cgtcgttaag cctttcgcaa atatttcacc
    36661 cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc atgccgtcaa aaaacgcctg
    36721 cacatcgtcc attgtttcgc gcaagttatg cttgcggaag aactcaacaa acatcgtcac
    36781 cgtcaaactt agtaacatca ccaccaccag atgcagttct gactgcatca gcccgacgtg
    36841 ggcgaggaac agcgagccga gcatcaggat taacggcatc acaggtaaaa tggcgtaata
    36901 gagcggcggg acattatcga gagttttttg ctctgcctgt tcgtgattga tatttttatc
    36961 ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc gccaccgaga taatgacgca
    37021 agaggcgacc ggaagctggt agtggaagaa gtatgtggca attttcattc ccgcgacctg
    37081 ggctgcaaaa atggagttcg tttccagaat cccccactca atggacatcg tggttgcgat
    37141 aaccgccacc gcagagaggc gacttactcc cagactcacc agcgtcggaa ataaggtgac
    37201 catcagcaac atacccagac cggaggcgct ggtaataaac tgcgccatga tttggccgat
    37261 gacgtaagtt gccgacagaa taatatacgg cgag
//
LOCUS       DAJTRH010000029        36599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTRH010000029 DAJTRH010000000
VERSION     DAJTRH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(156..1391)
                     /locus_tag="OO339_001631"
     CDS             complement(156..1391)
                     /locus_tag="OO339_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5926303.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO339_001632"
     tRNA            complement(1538..1614)
                     /locus_tag="OO339_001632"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OO339_001633"
     tRNA            complement(1657..1743)
                     /locus_tag="OO339_001633"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OO339_001634"
     tRNA            complement(1764..1839)
                     /locus_tag="OO339_001634"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OO339_001635"
     tRNA            complement(1898..1974)
                     /locus_tag="OO339_001635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OO339_001636"
     CDS             complement(2077..3462)
                     /locus_tag="OO339_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5926304.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO339_001637"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO339_001637"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5926305.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFECIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO339_001638"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO339_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5926306.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO339_001639"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO339_001639"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5926307.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO339_001640"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO339_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5926308.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO339_001641"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO339_001641"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5926309.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO339_001642"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO339_001642"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5926310.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO339_001643"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO339_001643"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5926311.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO339_001644"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO339_001644"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5926312.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO339_001645"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO339_001645"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5926313.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO339_001646"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO339_001646"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5926314.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO339_001647"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO339_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5926315.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO339_001648"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO339_001648"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5926316.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO339_001649"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO339_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5926317.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO339_001650"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO339_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5926318.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO339_001651"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO339_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5926319.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OO339_001652"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OO339_001652"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5926320.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OO339_001653"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OO339_001653"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5926321.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO339_001654"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO339_001654"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5926322.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OO339_001655"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OO339_001655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5926323.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO339_001656"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO339_001656"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5926324.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OO339_001657"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OO339_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5926325.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO339_001658"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO339_001658"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5926326.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO339_001659"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO339_001659"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5926327.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO339_001660"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO339_001660"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5926328.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO339_001661"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO339_001661"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5926329.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OO339_001662"
     CDS             complement(31267..32913)
                     /locus_tag="OO339_001662"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5926330.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OO339_001663"
     CDS             complement(32916..32966)
                     /locus_tag="OO339_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926331.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO339_001664"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO339_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5926332.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="OO339_001665"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="OO339_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="OO339_001666"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="OO339_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5926333.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="OO339_001667"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="OO339_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5926334.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(36398..36473)
                     /locus_tag="OO339_001668"
     tRNA            complement(36398..36473)
                     /locus_tag="OO339_001668"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="OO339_001669"
     tRNA            complement(36482..36558)
                     /locus_tag="OO339_001669"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8942 a        10017 c         9219 g         8421 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc attcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gatgtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggttttacga ccggcttac
//
LOCUS       DAJTRH010000030        35421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTRH010000030 DAJTRH010000000
VERSION     DAJTRH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(114..872)
                     /gene="deoR"
                     /locus_tag="OO339_001670"
     CDS             complement(114..872)
                     /gene="deoR"
                     /locus_tag="OO339_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5926335.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(930..1526)
                     /gene="ybjG"
                     /locus_tag="OO339_001671"
     CDS             complement(930..1526)
                     /gene="ybjG"
                     /locus_tag="OO339_001671"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5926336.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1811..3037
                     /gene="mdfA"
                     /locus_tag="OO339_001672"
     CDS             1811..3037
                     /gene="mdfA"
                     /locus_tag="OO339_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5926337.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3078..3362)
                     /locus_tag="OO339_001673"
     CDS             complement(3078..3362)
                     /locus_tag="OO339_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926338.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3448..4263)
                     /gene="ybjI"
                     /locus_tag="OO339_001674"
     CDS             complement(3448..4263)
                     /gene="ybjI"
                     /locus_tag="OO339_001674"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5926339.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4263..5471)
                     /locus_tag="OO339_001675"
     CDS             complement(4263..5471)
                     /locus_tag="OO339_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5926340.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5555..6091
                     /gene="rcdA"
                     /locus_tag="OO339_001676"
     CDS             5555..6091
                     /gene="rcdA"
                     /locus_tag="OO339_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5926341.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6266..7951)
                     /locus_tag="OO339_001677"
     CDS             complement(6266..7951)
                     /locus_tag="OO339_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5926342.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8221..8598
                     /locus_tag="OO339_001678"
     CDS             8221..8598
                     /locus_tag="OO339_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5926343.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8628..8885)
                     /gene="grxA"
                     /locus_tag="OO339_001679"
     CDS             complement(8628..8885)
                     /gene="grxA"
                     /locus_tag="OO339_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5926344.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9045..9332
                     /locus_tag="OO339_001680"
     CDS             9045..9332
                     /locus_tag="OO339_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5926345.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9316..10038
                     /gene="nfsA"
                     /locus_tag="OO339_001681"
     CDS             9316..10038
                     /gene="nfsA"
                     /locus_tag="OO339_001681"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5926346.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10099..11001
                     /gene="rimK"
                     /locus_tag="OO339_001682"
     CDS             10099..11001
                     /gene="rimK"
                     /locus_tag="OO339_001682"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5926347.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11089..11565
                     /locus_tag="OO339_001683"
     CDS             11089..11565
                     /locus_tag="OO339_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5926348.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11917..13029
                     /gene="potF"
                     /locus_tag="OO339_001684"
     CDS             11917..13029
                     /gene="potF"
                     /locus_tag="OO339_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5926349.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13124..14257
                     /gene="potG"
                     /locus_tag="OO339_001685"
     CDS             13124..14257
                     /gene="potG"
                     /locus_tag="OO339_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5926350.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14267..15220
                     /gene="potH"
                     /locus_tag="OO339_001686"
     CDS             14267..15220
                     /gene="potH"
                     /locus_tag="OO339_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5926351.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15217..16062
                     /gene="potI"
                     /locus_tag="OO339_001687"
     CDS             15217..16062
                     /gene="potI"
                     /locus_tag="OO339_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5926352.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16122..16610
                     /locus_tag="OO339_001688"
     CDS             16122..16610
                     /locus_tag="OO339_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5926353.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16651..17778
                     /gene="rlmC"
                     /locus_tag="OO339_001689"
     CDS             16651..17778
                     /gene="rlmC"
                     /locus_tag="OO339_001689"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5926354.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17953..18684)
                     /gene="artJ"
                     /locus_tag="OO339_001690"
     CDS             complement(17953..18684)
                     /gene="artJ"
                     /locus_tag="OO339_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5926355.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18976..19644)
                     /gene="artM"
                     /locus_tag="OO339_001691"
     CDS             complement(18976..19644)
                     /gene="artM"
                     /locus_tag="OO339_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5926356.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19644..20360)
                     /gene="artQ"
                     /locus_tag="OO339_001692"
     CDS             complement(19644..20360)
                     /gene="artQ"
                     /locus_tag="OO339_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5926357.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20367..21098)
                     /gene="artJ"
                     /locus_tag="OO339_001693"
     CDS             complement(20367..21098)
                     /gene="artJ"
                     /locus_tag="OO339_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5926358.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21116..21844)
                     /gene="artP"
                     /locus_tag="OO339_001694"
     CDS             complement(21116..21844)
                     /gene="artP"
                     /locus_tag="OO339_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5926359.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22062..22577)
                     /locus_tag="OO339_001695"
     CDS             complement(22062..22577)
                     /locus_tag="OO339_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5926360.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23226..24995
                     /locus_tag="OO339_001696"
     CDS             23226..24995
                     /locus_tag="OO339_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5926361.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25206..25529
                     /locus_tag="OO339_001697"
     CDS             25206..25529
                     /locus_tag="OO339_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5926362.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25526..26356
                     /gene="amiD"
                     /locus_tag="OO339_001698"
     CDS             25526..26356
                     /gene="amiD"
                     /locus_tag="OO339_001698"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5926363.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26353..27366)
                     /locus_tag="OO339_001699"
     CDS             complement(26353..27366)
                     /locus_tag="OO339_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5926364.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27465..28895)
                     /locus_tag="OO339_001700"
     CDS             complement(27465..28895)
                     /locus_tag="OO339_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5926365.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28906..29907)
                     /gene="ltaE"
                     /locus_tag="OO339_001701"
     CDS             complement(28906..29907)
                     /gene="ltaE"
                     /locus_tag="OO339_001701"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5926366.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29944..31662)
                     /gene="poxB"
                     /locus_tag="OO339_001702"
     CDS             complement(29944..31662)
                     /gene="poxB"
                     /locus_tag="OO339_001702"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5926367.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31795..32763)
                     /gene="hcr"
                     /locus_tag="OO339_001703"
     CDS             complement(31795..32763)
                     /gene="hcr"
                     /locus_tag="OO339_001703"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5926368.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32775..34427)
                     /gene="hcp"
                     /locus_tag="OO339_001704"
                     /gene_synonym="priS"
     CDS             complement(32775..34427)
                     /gene="hcp"
                     /locus_tag="OO339_001704"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5926369.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34571..>35421)
                     /gene="lysO"
                     /locus_tag="OO339_001705"
     CDS             complement(34571..>35421)
                     /gene="lysO"
                     /locus_tag="OO339_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5926370.1"
                     /translation="IPLRQQAALKAINQLLSWMVYLILFFMGISLAFLDNLASNLLAI
                     LHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRIAMALESLKLCGVVVIGF
                     AIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQIVLNRRGMIVAVVVVVS
                     SLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFGPVIGSAAFFNDLARELI
                     AIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMVPAAIVHGFILSLLVPIL
                     IAFFSA"
BASE COUNT         8799 a         8768 c         9097 g         8757 t
ORIGIN      
        1 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
       61 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      121 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      181 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      241 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      301 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      361 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      421 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      481 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      541 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      601 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      661 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      721 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      781 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
      841 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
      901 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
      961 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1021 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1081 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1141 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1201 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1261 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1321 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1381 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1441 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1501 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1561 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1621 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1681 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1741 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1801 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     1861 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     1921 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     1981 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2041 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2101 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2161 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2221 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2281 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2341 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2401 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2461 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2521 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2581 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2641 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2701 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2761 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     2821 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     2881 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     2941 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3001 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3061 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3121 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3181 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3241 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3301 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3361 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3421 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3481 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3541 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3601 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3661 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3721 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3781 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     3841 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     3901 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     3961 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4021 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4081 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4141 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4201 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4261 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4321 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4381 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4441 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4501 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4561 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4621 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4681 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4741 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4801 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     4861 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     4921 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     4981 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5041 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5101 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5161 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5221 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5281 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5341 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5401 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5461 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5521 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5581 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5641 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5701 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5761 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     5821 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     5881 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     5941 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6001 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6061 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6121 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6181 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6241 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6301 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6361 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6421 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6481 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6541 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6601 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6661 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6721 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6781 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     6841 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     6901 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     6961 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7021 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7081 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7141 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7201 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7261 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7321 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7381 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7441 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7501 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7561 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7621 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7681 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7741 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7801 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     7861 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     7921 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     7981 tgttgaaaga aatgctaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8041 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8101 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8161 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8221 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8281 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8341 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8401 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8461 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8521 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8581 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8641 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8701 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8761 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     8821 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     8881 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     8941 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9001 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9061 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9121 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9181 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9241 aggtggcaaa acgtcttgcc ccacagctga tgaactgtcc accgcaatat tcacgttcag
     9301 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg cgccattcgc
     9361 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9421 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9481 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9541 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9601 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9661 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9721 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9781 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
     9841 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
     9901 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
     9961 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10021 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10081 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10141 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10201 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta tttactacaa aggccgcaag
    10261 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10321 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10381 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10441 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10501 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10561 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10621 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10681 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10741 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10801 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    10861 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    10921 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    10981 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11041 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11101 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11161 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11221 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11281 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11341 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11401 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11461 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11521 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11581 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11641 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11701 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11761 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    11821 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    11881 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    11941 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12001 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12061 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12121 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12181 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12241 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12301 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12361 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12421 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12481 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12541 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12601 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12661 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12721 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgccagcgga tgccaaaaac
    12781 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    12841 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    12901 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    12961 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13021 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13081 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13141 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13201 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13261 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13321 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13381 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13441 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13501 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13561 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13621 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13681 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13741 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13801 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    13861 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    13921 tggcttggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    13981 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14041 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14101 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14161 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14221 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14281 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14341 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14401 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14461 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14521 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14581 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14641 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14701 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14761 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    14821 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    14881 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    14941 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15001 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15061 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15121 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15181 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15241 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15301 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggg cggctggtca
    15361 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15421 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15481 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15541 ccgctagtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15601 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15661 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15721 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15781 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    15841 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    15901 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    15961 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16021 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16081 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16141 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16201 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16261 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16321 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16381 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16441 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16501 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16561 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16621 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16681 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16741 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16801 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    16861 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    16921 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    16981 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17041 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17101 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17161 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17221 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17281 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17341 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17401 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17461 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17521 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17581 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17641 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17701 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17761 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    17821 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    17881 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    17941 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18001 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18061 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18121 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18181 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18241 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18301 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18361 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18421 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18481 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18541 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18601 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18661 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18721 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18781 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    18841 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    18901 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    18961 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19021 catcatcagc gtcagcaggc cgttgacgac caagtaaata atccctgccg caccgaacac
    19081 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19141 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19201 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19261 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19321 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19381 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19441 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19501 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19561 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19621 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19681 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19741 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19801 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    19861 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    19921 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    19981 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20041 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20101 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20161 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20221 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20281 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20341 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20401 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20461 tttccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20521 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20581 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20641 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20701 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20761 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    20821 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    20881 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    20941 gcctgcgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21001 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21061 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21121 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21181 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21241 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21301 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21361 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21421 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21481 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21541 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21601 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21661 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21721 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21781 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    21841 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttaa
    21901 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    21961 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22021 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22081 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22141 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22201 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22261 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22321 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22381 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22441 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22501 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22561 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22621 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22681 ctgattgggt ttgagtccat tgctggcgac ccactcaagg ttgctcgtag ctgacttact
    22741 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22801 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    22861 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    22921 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    22981 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23041 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23101 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23161 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23221 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23281 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat cccagtcgta
    23341 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23401 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23461 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23521 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23581 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23641 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23701 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23761 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    23821 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    23881 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    23941 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24001 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24061 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24121 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24181 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24241 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24301 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24361 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24421 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24481 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24541 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24601 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24661 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24721 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24781 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    24841 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    24901 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    24961 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25021 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25081 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25141 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25201 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25261 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25321 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25381 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25441 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25501 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25561 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25621 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25681 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25741 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25801 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    25861 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    25921 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    25981 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26041 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26101 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26161 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26221 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26281 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26341 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26401 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26461 atccagcgta aagtcaaaat tcaacttgga ggcgccgtag tgggtcagcg gcggctcttt
    26521 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26581 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26641 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26701 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26761 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    26821 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    26881 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    26941 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27001 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27061 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27121 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27181 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27241 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27301 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27361 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27421 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27481 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27541 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27601 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27661 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27721 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27781 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    27841 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    27901 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    27961 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28021 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28081 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28141 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28201 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28261 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28321 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28381 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28441 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28501 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28561 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28621 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28681 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28741 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28801 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    28861 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    28921 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    28981 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29041 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29101 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29161 caaccgtgcg acgttatttt ccagggcata catcccggcg gcagccagaa tcccggactg
    29221 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29281 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29341 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29401 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29461 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29521 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29581 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29641 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29701 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29761 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    29821 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    29881 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    29941 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30001 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30061 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30121 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30181 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30241 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30301 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30361 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30421 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30481 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30541 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30601 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30661 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30721 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30781 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    30841 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    30901 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    30961 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31021 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31081 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31141 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31201 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31261 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31321 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31381 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31441 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31501 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31561 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31621 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31681 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31741 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31801 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    31861 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    31921 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    31981 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32041 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32101 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32161 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32221 gcgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32281 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32341 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32401 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32461 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32521 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32581 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32641 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32701 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32761 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    32821 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    32881 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    32941 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33001 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33061 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33121 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33181 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33241 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33301 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33361 gcctgggcga tgactgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33421 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33481 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33541 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33601 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33661 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33721 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33781 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    33841 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    33901 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    33961 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34021 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34081 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34141 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34201 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34261 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34321 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34381 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34441 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34501 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34561 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34621 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34681 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34741 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34801 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    34861 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    34921 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    34981 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35041 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35101 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35161 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35221 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35281 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35341 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35401 cttgttggcg aagcggaatg a
//
LOCUS       DAJTRH010000031        35155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTRH010000031 DAJTRH010000000
VERSION     DAJTRH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..971)
                     /locus_tag="OO339_001706"
     CDS             complement(<1..971)
                     /locus_tag="OO339_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5926371.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            1063..1149
                     /locus_tag="OO339_001707"
                     /pseudo
     CDS             1063..1149
                     /locus_tag="OO339_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1554..2030)
                     /locus_tag="OO339_001708"
     CDS             complement(1554..2030)
                     /locus_tag="OO339_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5926372.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(2089..3378)
                     /gene="bioA"
                     /locus_tag="OO339_001709"
     CDS             complement(2089..3378)
                     /gene="bioA"
                     /locus_tag="OO339_001709"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5926373.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3465..4505
                     /gene="bioB"
                     /locus_tag="OO339_001710"
     CDS             3465..4505
                     /gene="bioB"
                     /locus_tag="OO339_001710"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5926374.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4502..5656
                     /gene="bioF"
                     /locus_tag="OO339_001711"
     CDS             4502..5656
                     /gene="bioF"
                     /locus_tag="OO339_001711"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5926375.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5643..6398
                     /gene="bioC"
                     /locus_tag="OO339_001712"
     CDS             5643..6398
                     /gene="bioC"
                     /locus_tag="OO339_001712"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5926376.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6391..7068
                     /gene="bioD"
                     /locus_tag="OO339_001713"
     CDS             6391..7068
                     /gene="bioD"
                     /locus_tag="OO339_001713"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5926377.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7647..9668
                     /gene="uvrB"
                     /locus_tag="OO339_001714"
     CDS             7647..9668
                     /gene="uvrB"
                     /locus_tag="OO339_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5926378.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9860..10768)
                     /gene="yvcK"
                     /locus_tag="OO339_001715"
     CDS             complement(9860..10768)
                     /gene="yvcK"
                     /locus_tag="OO339_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5926379.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      11033..11177
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11165..12154
                     /gene="moaA"
                     /locus_tag="OO339_001716"
     CDS             11165..12154
                     /gene="moaA"
                     /locus_tag="OO339_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5926380.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12176..12688
                     /gene="moaB"
                     /locus_tag="OO339_001717"
     CDS             12176..12688
                     /gene="moaB"
                     /locus_tag="OO339_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5926381.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12691..13176
                     /gene="moaC"
                     /locus_tag="OO339_001718"
     CDS             12691..13176
                     /gene="moaC"
                     /locus_tag="OO339_001718"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5926382.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13169..13414
                     /gene="moaD"
                     /locus_tag="OO339_001719"
     CDS             13169..13414
                     /gene="moaD"
                     /locus_tag="OO339_001719"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5926383.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13416..13868
                     /gene="moaE"
                     /locus_tag="OO339_001720"
     CDS             13416..13868
                     /gene="moaE"
                     /locus_tag="OO339_001720"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5926384.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            14005..14709
                     /locus_tag="OO339_001721"
     CDS             14005..14709
                     /locus_tag="OO339_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5926385.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14914..15627
                     /locus_tag="OO339_001722"
     CDS             14914..15627
                     /locus_tag="OO339_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5926386.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15663..16619)
                     /locus_tag="OO339_001723"
     CDS             complement(15663..16619)
                     /locus_tag="OO339_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5926387.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16619..17860)
                     /gene="clsB"
                     /locus_tag="OO339_001724"
     CDS             complement(16619..17860)
                     /gene="clsB"
                     /locus_tag="OO339_001724"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5926388.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17857..18618)
                     /locus_tag="OO339_001725"
     CDS             complement(17857..18618)
                     /locus_tag="OO339_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5926389.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18751..19161
                     /locus_tag="OO339_001726"
     CDS             18751..19161
                     /locus_tag="OO339_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5926390.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(19123..20229)
                     /locus_tag="OO339_001727"
     CDS             complement(19123..20229)
                     /locus_tag="OO339_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5926391.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20240..21373)
                     /locus_tag="OO339_001728"
     CDS             complement(20240..21373)
                     /locus_tag="OO339_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5926392.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21366..23102)
                     /locus_tag="OO339_001729"
     CDS             complement(21366..23102)
                     /locus_tag="OO339_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5926393.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(23095..24090)
                     /gene="hlyD"
                     /locus_tag="OO339_001730"
     CDS             complement(23095..24090)
                     /gene="hlyD"
                     /locus_tag="OO339_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5926394.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(24093..24764)
                     /gene="cecR"
                     /locus_tag="OO339_001731"
     CDS             complement(24093..24764)
                     /gene="cecR"
                     /locus_tag="OO339_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5926395.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24993..26180
                     /gene="rhlE"
                     /locus_tag="OO339_001732"
                     /pseudo
     CDS             24993..26180
                     /gene="rhlE"
                     /locus_tag="OO339_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26387..26869)
                     /gene="ybiA"
                     /locus_tag="OO339_001733"
     CDS             complement(26387..26869)
                     /gene="ybiA"
                     /locus_tag="OO339_001733"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5926396.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26989..29139
                     /gene="dinG"
                     /locus_tag="OO339_001734"
     CDS             26989..29139
                     /gene="dinG"
                     /locus_tag="OO339_001734"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5926397.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29167..30129
                     /gene="ybiB"
                     /locus_tag="OO339_001735"
     CDS             29167..30129
                     /gene="ybiB"
                     /locus_tag="OO339_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5926398.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30270..31355
                     /gene="hcxB"
                     /locus_tag="OO339_001736"
     CDS             30270..31355
                     /gene="hcxB"
                     /locus_tag="OO339_001736"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5926399.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31584..31844)
                     /locus_tag="OO339_001737"
     CDS             complement(31584..31844)
                     /locus_tag="OO339_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5926400.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(32109..32375)
                     /gene="ybiI"
                     /locus_tag="OO339_001738"
     CDS             complement(32109..32375)
                     /gene="ybiI"
                     /locus_tag="OO339_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5926401.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32449..33126)
                     /gene="ybiX"
                     /locus_tag="OO339_001739"
     CDS             complement(32449..33126)
                     /gene="ybiX"
                     /locus_tag="OO339_001739"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5926402.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33168..35155)
                     /locus_tag="OO339_001740"
                     /pseudo
     CDS             complement(33168..35155)
                     /locus_tag="OO339_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8326 a         9008 c         9501 g         8320 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatccgtgg gccgtggtag tccggcgaag aggttaatct ttgcagggct
      181 tgcagagtgc gagtcgacaa tggattgtta taaatattaa catctgcata aatgtgcaga
      241 ttcatgatac tgtctggcaa tgtgttcagc tgattgttag ggatatatag tgattgcaga
      301 cgttgtggaa gttcgggcag tctgtgtagt tggttttccc caacgtttaa aagcctcaga
      361 ttttgaggaa ggggcggtag ctcttgtaat ctgttatcaa aagcacttag ttcctgtagt
      421 tcacatggta attctggtaa ggcagtaagc tggttgttgc caacagaaag tttttgtaga
      481 gccgggggaa gatcaggtag cgtctccagg ctattgagaa gggctgagag tgactgcaag
      541 gaagatggaa gagaaggcaa acatgttaaa actctgttaa gtgaaacatc aagagcgacc
      601 aggtgaggcg gaagagccgg gagcctgctc agtctgttat cgctggcttt aagtacagtt
      661 aaggacggag ggagttctgg cagagagcgt agctcattgc tagagatgtt aagttcttgt
      721 atgtgcgggg gcaggtatgg gagagagcgt aatctgagta aacttaaatt gagggctggc
      781 tcttgaaaag ccagacatat tttcagtaat cgaactgcct gtgttcggtc ttctgttgca
      841 gcaccttcct tggcccagtt atcccagatg cggtgataat ccgtgatatt ttgctcttct
      901 acggataaac gggatataca tcctaaacca caaaaggagc ggaaaaaaga aggattgtgg
      961 gcaggtttca tttattattg atcctcattg tttatatatt aatatattcg tgcaaatgga
     1021 gtgaggattc tttaaaaaaa cgcgcctgta aatagttaag aacaggaaga actgttgggg
     1081 atgtggctgg ccgaaaatga aggggtgaag ttctggctca atgtgctgac tgaactgaaa
     1141 aaacgcggtt ggtcgcccta ccttttcatc aagccagtag tctgcccacc acagcatcat
     1201 ttctctgcgc ttatcgagat actgagcatg gttgtaaatt cagctaaccc agtgcgttga
     1261 caaaaaatta gcgcaagagg acaaaaaatc accttgcgct aatgctctgt atcaggtcac
     1321 taataccatc taagtggttg attcatagtg actggatatg ttgtgttttg cagtattatg
     1381 cagtctattt tttaagctaa atgtattata atgcattgat attaatgatt tttactgttt
     1441 ctcggtcagc ttttttatac taacttgagc gaaacgggaa ggtaaaaaga caaaaagttg
     1501 tttttaatac ctttaagtga taccagatgg cattgcgcca tctggcagag tgattaacta
     1561 aacatcgcgg taatcgatgc gcttgccaga gagtggaaat gaacgttaaa cccgaccatc
     1621 gcgccgctgg ctccttcatc gacatcaata cgctctacat ccagcgcgtg aacggtaaaa
     1681 atgtagcgat gggtttcgcc tttcggcggc gctgcgccat cgtatccggt tttaccaaaa
     1741 tcggtacgcg tctgcaaaac gccgtctggc attgctacca gaccagagcc aaacccttgc
     1801 ggtaatacgc gggtatcagc gggtaaatta acaactaccc agtgccacca gccggagccg
     1861 gttggcgcat ccgggtcgta gcaggtgaca acaaaacttt tcgttcccgc aggaacatca
     1921 tcccacgcca gatgcggtga aatattatcg ccatcgtaac ccatgccgtt aaagacatga
     1981 cgatgcggca acttatcgcc atcgcgcaga tcgttactga tgagtttcat gaaccctcct
     2041 ttcttgtttg cagaaagtgt agccagaaac cctcacgctg acttcccgtt attggcaaaa
     2101 aaatgtttca tcctgtaccg cgcggttaac cgctgcggtc agacgctgca actgttgcgg
     2161 gagaataata tagggcggca tcaggtaaat cagtttgcca aaaggccgga tccatacccc
     2221 ctgttcgaca aagaattttt gcagcgccgc catattcacc ggacgagtgg tttcgaccac
     2281 accgattgcc cccagtacgc gcacatcggc aaccatttcg gcatcacggg ctggtgccag
     2341 ttgctcgcgc agctgcactt caatagccgc cacctgctgc tgccattcgc cggattcgat
     2401 aatcgctagg ctggcgtttg ctgccgcgca ggccagcgga ttgcccataa aagttggccc
     2461 atgcataaag cagccggctt cgccgttact gatggtttct gcaacctcgc gcgtggtaag
     2521 tgtggcggaa agggtcattg tgccgccggt taaggcttta ccgaggcaca aaatgtccgg
     2581 cgcgatttct gcatattcac aggcaaacag tttgccggta cgaccaaatc cggtggcgat
     2641 ctcgtcggca atcagcaaga taccttcgcg atcgcacatt ttgcggattc gttttaacca
     2701 ttccggatgg tacatgcgca tcccgcctgc gccctggaca atcggctcaa tgatcaccgc
     2761 tgcgatttca tgacgatgcg ccgccatcag gcgggcaaaa ccaaccatat cgcgttcatc
     2821 ccattcacca tccatgcggc tttgcggggc gggagcaaac aagttttctg gcagatagcc
     2881 tttccacagg ctgtgcattg agttgtccgg atcgcacacc gacatcgcgc caaaggtatc
     2941 gccatgataa ccattgcgga aggtcagaaa acgctggcgc gcttcgcctt tggcttgcca
     3001 gtactgcaac gccattttca tcgccacttc caccgctacg gaaccggagt ccgcgagaaa
     3061 aacgcactcc agcggttgcg gcgtcattgc caccagtttg cggcatagct cgatggcggg
     3121 cgcatgggtg ataccgccaa acatcacatg cgacatggca tcaatttgcg tcttcatcgc
     3181 cgcattaagc tgcgggtgat tgtagccgtg gatcgccgcc caccaggacg acataccgtc
     3241 aaccaggcgt ctgccgtcag acaaaattag ctcgcaacct tcggcgctcg ccaccggata
     3301 aaccggcaga ggggaggtca tggatgtgta tgggtgccag atatggcgtt ggtcaaaggc
     3361 aagatcgtcc gttgtcataa tcgacttgta aaccaaattg aaaagattta ggtttacaag
     3421 cctacaccga attaacaaca aaaaacacgt tttggagaag ccccatggct caccgcccac
     3481 gctggacatt gtcgcaagtc acagaattat ttgaaaaacc gttgctggat ctgctgtttg
     3541 aagcgcagca ggtgcatcgc cagcatttcg aacctcgtca ggtgcaggtc agcacgttgc
     3601 tgtcgattaa gaccggagct tgtccggaag attgcaaata ctgcccgcaa agctcgcgct
     3661 acaaaaccgg gctggaagcc gagcggttga tggaagttga acaggtgctg gagtcggcgc
     3721 gcaaagcgaa agcggcagga tcgacgcgct tctgtatggg cgcggcatgg aagaatcccc
     3781 acgaacgcga tatgccgtac ctggaacaaa tggtgcaggg ggtaaaagcg atggggctgg
     3841 aggcgtgtat gacgctgggc acgttgagtg aatctcaggc gcagcgcctc gcgaacgccg
     3901 ggctggatta ctacaaccac aacctggaca cctcgccgga gttttacggc aatatcatca
     3961 ccacacgcac ttatcaggaa cgcctcgata cgctggaaaa agtgcgcgat gccgggatca
     4021 aagtctgctc aggtggcatt gtgggcttag gcgaaacggt aaaagatcgc gccggattat
     4081 tgctgcaact ggcaaacctg ccgacgccgc cggaaagcgt accaatcaac atgctggtga
     4141 aggtgaaagg cacgccgctt gccgataacg atgacgttga tgcctttgat tttattcgca
     4201 ccattgccgt agcgcggatc atgatgccga cctcttacgt gcgcctttct gccggacgcg
     4261 agcagatgaa cgaacagact caggcgatgt gctttatggc aggcgcaaac tcgattttct
     4321 acggttgcaa actgctgacc acgccgaatc cggaagaaga taaagacctg caactgttcc
     4381 gcaaactggg gctaaatccg cagcaaactg ccgtgctggc aggggataac gaacaacagc
     4441 aacgtcttgg acaggcgctg atgaccccgg acaccgacga atattacaac gcggcagcat
     4501 tatgagctgg caggagaaaa tcaacgcggc gctcgatgcg cggcgtgctg ccgatgccct
     4561 gcgtcgccgt tatccggtgg cgcaaggagc cggacgctgg ctggtggcgg atgatcgcca
     4621 gtatctgaac ttttccagta acgattattt aggtttaagc catcatccgc aaattatccg
     4681 tgcctggaag cagggggcgg agcaatttgg catcggtagc ggcggctccg gtcacgtcag
     4741 cggttatagc gtggtgcatc aggcgctgga agaagaactg gccgagtggc ttggctattc
     4801 gcgggcgctg ctgtttatct ctggttttgc cgcgaatcag gcagtcattg ccgcgatgat
     4861 ggcgaaagag gaccgtattg ttgccgaccg gcttagccat gcctcattgc tggaggctgc
     4921 cagtttaagc ccgtcgcagc ttcgccgttt tactcataac gatgtcgctc atctggcgcg
     4981 actgcttgct tccccctgtc cggggcagca actggtggtg acagaaggcg tgttcagcat
     5041 ggacggcgat agcgcgccac tggcggaaat ccagcaggta acgcaacagc acaatggctg
     5101 gttgatggtc gatgatgccc acggcacggg cgttatcggg gagcaggggc gcggcagctg
     5161 ctggctgcaa aaggtaaaac cagaattgct ggtagtgact tttggcaaag gatttggcgt
     5221 cagtggggct gcggtgcttt gctcaagtac ggtggcggat tatctgctgc aattcgcccg
     5281 ccaccttatc tacagcacca gtatgccgcc cgctcaggcg caggcattac gtgcgtcgct
     5341 ggcggtcatt cgccgtgatg agggggatgc acggcgcgaa aaactggtgt cactcattgc
     5401 gcgttttcgt gccggagtac aggatttgcc gtttacgctg gctgattcat gcagcgccat
     5461 ccagccattg atcgtcggtg ataacagccg tgcgttacaa ctggcagaaa aactgcgcca
     5521 gcaaggctgc tgggtcacgg cgattcgccc gccaaccgta cccgctggta ctgcgcgact
     5581 gcgcttaacg ctaaccgctg cgcatgaaat gcaggatatc gaccgtctgc tggaggtgct
     5641 gcatggcaac ggttaataaa caagccattg cagcggcatt tggtcgggca gccgcacact
     5701 atgagcaaca tgcagatcta cagcgccaga gtgctgacgc cttactggca atgcttccac
     5761 agcgtaaata cacccacgta ctggacgcgg gttgtggacc tggctggatg agccgccact
     5821 ggcgggaacg tcacgcgcag gtgacggcgt tagatctctc gccgccaatg cttgcgcaag
     5881 cccgccataa agatgccgca gatcattatc tggcaggaga tatcgaatcc ctgccgttag
     5941 cgactgcgac gttcgatctt gcatggagca atctcgcggt gcagtggtgc ggtaatttat
     6001 ccacggcact ccgcgagctg tatcgggtgg tgcgccccgg aggcgtggtc gcgtttacca
     6061 cgctggtgca gggatcgtta cccgaactgc atcaggcgcg gcaggcggtg gacgagcgtc
     6121 cgcatgctaa tcgcttttta ccgccagatg aaatcgaaga gtcgctgaac ggcgtgcgtt
     6181 atcaacatca tattcagctc atcacactat ggtttgatga tgcgctcagt gccatgcgtt
     6241 cgctgaaagg catcggtgct acgcatcttc atgaagggcg cgacccgcga atattaacgc
     6301 gttcgcagtt gcagcgattg caactggcct ggccgcaaca acaggggcga tatcctctga
     6361 cgtatcatct ttttttggga gtgattgctc gtgagtaaac gttattttgt caccggaacg
     6421 gataccgaag tggggaaaac cgtcgccagt tgtgcacttt tacaagccgc gaaggcagta
     6481 ggttatcgga cggcaggtta taaaccggtc gcctctggca gcgaaaagac cccggaaggc
     6541 ttacgtaata gtgacgcgct ggcgttacag cacaacagca gcctgcagct ggattacgca
     6601 acggtaaatc cttacacctt cgcagaatcc acctcgccgc acatcatcag cgcccaagag
     6661 ggcaggccga tagaatcatt ggtaatgagc gccggattac gcgcgcttga acaacaggct
     6721 gactgggtgt tagtggaagg tgctggcggc tggtttacgc cgctttctga cactttcact
     6781 tttgcagatt gggtaacaca ggaacaactg ccggtgatac tggtagttgg tgtgaaactc
     6841 ggctgtatta atcacgcgat gttgactgcg caggcaatac aacacgccgg actgactctg
     6901 gcgggttggg tggcgaacga tgttacgcct ccgggaaaac gtcacgctga atatatgacc
     6961 acgctcaccc gcatgattcc cgcgccgctg ctgggagaga tcccctggct tgcagaaaat
     7021 ccagaaaatg cggcaaccgg aaagtacata aaccttgcct tgttgtagcc attctgtatt
     7081 tggttaaatt gcgagcgaga tcgcgtcttc gattgactgc aatttaacca attaaattct
     7141 aaaataatca cgaaaaaaat tttacttccg cctcatgcgg cgaatgtggg aattgcccag
     7201 gcggcggggg ataggggctg gagacagtta tccactattc ctgtggataa ccatgtgtat
     7261 tagagttaga aaacacgagg caagcgagag aatacgcggc ttgcacgcga attggcgtta
     7321 aagacggctc aaagaaatat cttttatttt ttaactggtt agataaatgc aatggcagtc
     7381 actgaacagg catctcttgc cataaaactg tcatcactca tcttgacaaa tgttaaaaaa
     7441 gccgttgctt tggggataac ccggtaaggc tggagtttta tctcgccaca gagtaaattt
     7501 tgctcatgat tgacagcgga gtttacgctg tatcagaaat attatggtga tgaactgttt
     7561 ttttatccag tataatttgt tggcataatt aagtacgacg agtaaaatta catacctgcc
     7621 cgcccaactc cttcaggtag cgactcatga gtaaaccgtt caaactgaat tccgctttta
     7681 aaccttctgg cgatcagcca gaggcgattc gacgtctcga agaggggctg gaagatggcc
     7741 tggcgcacca gacgttactt ggcgtgactg gctccgggaa aaccttcacc attgccaatg
     7801 tcattgctga ccttcagcgc ccaaccatgg tacttgcgcc caacaaaacg ctggcggccc
     7861 agctgtatgg cgaaatgaaa gagttcttcc cggaaaacgc ggtggagtat ttcgtctcct
     7921 actacgacta ctatcagccg gaagcctatg taccgagttc cgacaccttc attgagaaag
     7981 atgcctcggt taacgaacat attgagcaga tgcgtttgtc cgccaccaaa gcgatgctgg
     8041 agcggcgtga tgtgattgta gtggcgtctg tttccgcgat ttatggtctg ggcgatcctg
     8101 atttatatct caagatgatg ctccatctca cggtcggtat gattatcgat cagcgcgcga
     8161 ttctgcgccg actggcggag ctgcaatacg ctcgtaatga tcaagcattc cagcgtggta
     8221 ctttccgcgt tcgtggcgag gtgatagata tcttcccggc agaatcggat gacattgcac
     8281 ttcgcgtgga actgtttgac gaggaagtgg aacgattgtc gttatttgac ccgctgaccg
     8341 ggcagattgt ttccactatt ccacgtttta ccatctaccc gaaaacgcac tacgtcacac
     8401 cgcgcgagcg catcgtacag gcgatggagg agatcaaaga agaactggcc gccagacgca
     8461 aagtgctgtt ggaaaacaac aaactgctgg aagagcagcg gctgacccag cgtacccagt
     8521 ttgatctgga gatgatggac gagctgggct actgttcggg gattgaaaac tactcgcgct
     8581 tcctctccgg tcgtggaccg ggtgagccac cgccgacgct gtttgattac ctgcctgccg
     8641 atgggctgct ggtcgtcgat gaatctcacg tcaccattcc acaaattggc ggcatgtatc
     8701 gcggtgaccg ggcgcgtaaa gagacactgg tggagtacgg cttccgcctg ccatcagcgc
     8761 tggataaccg tccgcttaag tttgaagagt tcgaagcatt agcgccgcaa accatctatg
     8821 tttcggcgac gccgggtaat tacgagctgg aaaaatccgg cggcgatgtg gtggatcagg
     8881 tggtgcgtcc aaccggattg cttgacccga ttatcgaagt gcggccggtg gcgacacagg
     8941 ttgatgatct tctttcggag attcgtcagc gagcggcaat taacgaacgc gtgctggtca
     9001 ccacactgac caagcgaatg gcggaagatc ttaccgaata tctcgaagaa cacggtgagc
     9061 gcgtgcgtta tcttcactca gatatcgaca ccgtcgagcg tatggagatt atccgcgact
     9121 tgcgtctggg tgagttcgac gtattggtag ggatcaactt actgcgtgaa ggtctggata
     9181 tgccggaagt gtcgctggtg gcgatcctcg acgctgacaa agaaggcttc ctgcgttccg
     9241 aacgttcgtt gatccagacc attggtcgtg cggcacgtaa cgttaacggt aaagcgattc
     9301 tctacggcga taggagcact ccatcaatgg cgaaagcgat tggcgaaacc gaacgtcgcc
     9361 gtgagaaaca gcagaagtac aacgaggaac acggcattac gccgcaaggc ttgaacaaga
     9421 aagtggtcga tatcctggcg ctggggcaga acattgccaa aaccaaagcg aagggcagag
     9481 gaaaatcgcg cccgattgtt gagccggata atgtgccgat ggatatgttg ccgaaagcgt
     9541 tgcagcagaa aatccatgaa ctggaagggc tgatgatgca acacgcgcag aatctggagt
     9601 tcgaagaagc ggcacaaatt cgtgaccagt tgcatcagtt gcgtgagttg tttattgccg
     9661 catcgtaaca ggatagcgaa gaagactgat gacaaacgga aaacagtctg atgcgctacg
     9721 cttatcaggc ctacattttc tccgcaatat attgaatttg cgcggtttgt aggccggata
     9781 aggcgttcac gccgcatccg gcataaacaa cgcgcacttt gtcttcaata ggaagccgga
     9841 attttccttc ggatttccgt taacctaaag cctgtaacgc cttttccagc gcgttatgta
     9901 acaactggcg gtcatgacga tacggaatat cgctggcctc cagtacctcc tggatcacaa
     9961 tccgctcttt caccgccgag acatccactt ttggcccgac gatgaccgca tcaatgacct
    10021 ttttaccaac atactgctcc ataattgcca gcttgctttc cagcttcaaa ttagccgcag
    10081 gtaaactcaa ctcacgcccc agattgccga tataaaccat cggcgctggc gtgcggcgta
    10141 atgcctgggc gatttccttc agcagcagaa ttggcatcag gctggtataa aaactgccag
    10201 gcccaataat gatgagatcc gcttcattga tagcgtgaac cgcctcacgc gttgcgggta
    10261 cattaggcgt taacaataac tcttgaatcg gcgtagttaa ctggtcgata ttgacctcgc
    10321 cgtaaacttc atgcccctga tcgtcaatcg ccatcagatc aacaggatgc tctgacattg
    10381 gaatcaaatg cgtatccact ttcagcagat tacgaattaa attgatggct tccagaggcc
    10441 gcacgctaag gtgatccagc gcctttaaca tcaagtttcc gagattatga ccggaaagtt
    10501 cgccattgcc gccaaaacgg tattcaaaca tcgcggaggc gacgttcggt tccgttatca
    10561 gctggttgag gcagttgcgc atatcgcccc aagcaatgcc gccttctgaa cggcgaatac
    10621 gccccgtcga gccaccatta tccgtggtgg tgacgatacc cgttaaacga gaacccaaag
    10681 acgaaagtga tgagagaacg cgtcccagtc catgccctcc gccgagagca acgacacgat
    10741 caagatcagc cagcgtacga ttgcgcatat agtttcctgg agtcagatta tccgcgctac
    10801 agtagcgcaa attgcgggga aacagcaatt aacctgccaa tattgaggat acaaactatt
    10861 ttcttctatc tctaaaggac gatgcacgct atgcctccct gatgatgtat atcaaagtaa
    10921 aaccgccatt ttcccttatt ctgtagcgaa atagcacgat catgacgcta tatacatgat
    10981 tacatagcga aagtgtggat ggtaaaaatc tcatttacac gctagtatcg gcttaaccac
    11041 taaacactct agcctctgca cctgggtcaa ctgatacggt gctttggccg tgacaatgct
    11101 cgtaaagatt gccaccaggg cgaaggaaga aatgacttcg cctcccgtat ctggaaaggt
    11161 gtacatggct tcacaactga ctgatgtatt tgcgcgtaag ttttactact tgcgcctgtc
    11221 gattaccgat gtgtgtaact ttcgttgcac ctactgcctg ccggatggct acaaaccgag
    11281 cggcgtcacc aataaaggct ttcttaccgt cgatgaaatt cgccgggtta cgcgcgcctt
    11341 cgccagtctg ggcaccgaaa aagtgcgcct gacaggagga gagccgtctt tacgccgcga
    11401 ctttaccgat atcatcgccg ctgtgcggga aaacgacgct atccgccaga ttgcggtcac
    11461 caccaatggt taccgtctgg aacgcgatgt ggcgaactgg cgcgatgcgg gacttactgg
    11521 cattaacgtc agtgtcgaca gtttggacgc ccgccagttt cacgctatta ccgggcagga
    11581 taaattcaac caggtcatgg cagggattga tgctgcattt gaggccggtt ttgagaaggt
    11641 caaagtcaat accgtgctga tgcgtgatgt taatcatcac cagctcgaca cctttctgaa
    11701 ctggatccag catcgcccta tccagctgcg tttcatcgaa ctgatggaaa cgggcgaggg
    11761 cagcgagctc ttccgtaagc atcacatctc tggtcaggtt ctgcgtgacg agctactgcg
    11821 tcgcggctgg atccaccaat tacgtcaacg cagcgacggt cccgcgcaag tcttttgcca
    11881 tccagattac gccggagaga ttggccttat catgccgtat gaaaaagact tctgcgccac
    11941 ttgcaaccgc ctgcgcgttt cctccattgg taaactccat ctctgcctgt ttggtgaagg
    12001 cggcgttaac ctgcgcgatc tgctggaagg cgatacccag caacaggcgc tggaagcgcg
    12061 tatttcagcg gcgctgcggg agaagaaaca gacccatttc ctgcatcaaa acaacaccgg
    12121 tattacgcaa aacttatcgt acattggcgg ctaaaacgtc aaaaggagag atcagatgag
    12181 tcaggtaagc actgaattta tcccgacccg tattgctatt cttacggttt ctaatcgtcg
    12241 cggtgaagaa gacgatacct ccggtcacta tctgcgcgat tcggcgcaag aagcgggcca
    12301 tcacgttgtc gatgaagcca ttgtgaaaga aaaccgctac gctattcgcg ctcaggtatc
    12361 tgcgtggatc gccagcgacg atgtacaagt ggtattgatt acgggtggta ctggcctgac
    12421 ggaaggtgat caggctcccg aagcgctgct gccgttgttc gaccgtgagg ttgaaggttt
    12481 tggtgaagtg ttccgtatgt tgtcgtttga agagattggc acttccacgt tgcaatctcg
    12541 tgcggtagcg ggcgtcgcta acaaaacgct gattttcgcc atgccgggtt cgaccaaagc
    12601 gtgccgtacc gcatgggaaa atatcatcgc gccgcagctg gatgcccgta cgcgtccgtg
    12661 taatttccat ccacatttga agaaataagt atgtcgcaac tgacccatat caacgccgct
    12721 ggcgaagcgc acatggtgga tgtctccgcc aaagcggaaa ccgtgcgtga agcgcgggcg
    12781 gaagcctttg tcaccatgcg cagcgagacg ctggcgatga ttattgatgg tcgccaccac
    12841 aaaggcgacg tatttgccac tgcgcgtatt gccggtattc aggcggcaaa acgcacttgg
    12901 gatctgatcc cgctgtgtca cccgctgatg ctcagcaaag ttgaagtcaa tttacaggcc
    12961 gagccggagc acaatcgggt gcgtatagaa accttatgcc gcctgaccgg gaaaaccggt
    13021 gtcgaaatgg aagcgttaac cgcggcctcc gtggcggcgc tgaccattta tgacatgtgc
    13081 aaagcggtgc aaaaagatat ggtgattggt ccggtacgtt tgctggcgaa gagcggcggc
    13141 aagtcgggtg actttaaggt ggaagcggat gattaaagtt ctttttttcg cccaggtgcg
    13201 cgagttggtg ggaacagatg caaccgaagt ggctgcggat tacccaaccg ttgaagcgtt
    13261 acgccagcac ctggctgcgc agagcgatcg ctgggcgctg gcgctggaag atggcaaatt
    13321 gctggctgcc gtcaaccaga cgctggtgag ttttgaccat ccgctgactg acggcgacga
    13381 agtagctttc ttcccgccgg taaccggagg ttaagatggc agaaaccaaa attgttgtcg
    13441 gcccgcagcc gttcagcgta ggagaagagt acccgtggct ggcggagcgt gacgaagacg
    13501 gtgcggtagt caccttcacc ggaaaggtgc gcaaccataa cctgggcgac agcgtcaaag
    13561 cattaaccct cgaacactat ccggggatga ctgaaaaagc actggcagaa attgttgatg
    13621 aagcgcgtaa ccgctggccg ctggggcgcg tcacggtgat tcaccgcatc ggggaattat
    13681 ggccgggcga tgaaatcgtt tttgtcggtg tcaccagtgc gcatcgcagc agtgcgtttg
    13741 aagccgggca gtttattatg gattatctca aaacccgcgc accgttctgg aagcgcgaag
    13801 ccacgccgga aggcgaccgc tgggttgaag cccgggagag cgatctgcag gcggcaaaac
    13861 gctggtagtt ttttgttagc cggataaggc accgctgcgt ccggcaattt caccgctcag
    13921 tacatgcgtt tacgctgcgt cgatgcacag cctcatcatt ttgcagtatc cttaagatat
    13981 tccttatatc ttcaggagat cgtcatggac agattcccac gttctgattc aatcgtacaa
    14041 ccctgggctg gcttgcaaac ttatatggcg caagtctatg gctggatgac cgttggcttg
    14101 ttgctgaccg catttgttgc ctggtatgcg gctaattccg cggccgtgat ggagctgttg
    14161 ttcactaacc gtgtcttttt aatcggtctg atcatcgcgc aattagcatt ggttattgtg
    14221 ttatcagcga tgattcaaaa gctgagcgca ggtgtaacga cgatgctctt tatgctttat
    14281 tcggcgctga cgggtcttac gctttccagt atattcattg tctataccgc tgcttctatc
    14341 gccagtactt tcgtcgttac tgccgggatg ttcggcgcaa tgagcctgta cggttacacc
    14401 acgaagcgcg atttaagtgg ctttggcaat atgctgttta tggcgttaat cggcattgtg
    14461 ctggcatcgc tggtcaactt ctggctgaaa agcgaagcat tgatgtgggc agttacctac
    14521 atcggcgtga ttgtctttgt cggattgacg gcgtatgaca ctcagaaatt gaaaaatatg
    14581 ggtgagcaga ttgatacccg cgacacgtcg aacctgcgca aatattccat tcttggtgcg
    14641 ttaaccttgt atctggactt catcaacctg ttcctgatgt tgttacggat cttcggcaac
    14701 cgccgttaat tctttttcac catcactatc ccggccatgt gtcgggattt ttttcttact
    14761 aaaaacgaca cttaacaagt accaggtata aaatatgcct tatgagcgag cggaatcgtt
    14821 ttccgtatcc ataaatacaa aagtattttg tatgcgtggt tttaaattta ttgatggtga
    14881 atattaatgt tttttctaag gacaactaaa agaatggaat catactcgca aaacagcaat
    14941 aaattagatt ttcagcacga ggccaggata ttaaacggta tatggctcat taccgcttta
    15001 ggtttggtgg caaccgcagg actagcctgg ggagctaagt atctcgaaat tacggcaacc
    15061 aaatatgatt caccaccaat gtatgtcgcc atagggttat tattactttg tatgtatggc
    15121 ttaagtaagg atatcaacaa gataaatgcc gccatcgcgg gcgtaatata tctgttttta
    15181 ctctctttgg tggcgattgt cgttgcaagt ttagttcctg tatatgccat tatcatcgtg
    15241 ttcagcactg cgggcgcgat gtttttaatc agtatgctgg ccggtttatt atttaatgtt
    15301 gatcctggtt ctcaccgttt tatcattatg atgacgttga cagggttggc cctggtaatc
    15361 atcgtgaatg cggcattaat gagtgaacgg cccatttggg taataagttg cttaatgatt
    15421 gtgttatggc caggcattat ctcgcatgga cgaaataagc tccttgaact ggcaggaaaa
    15481 tgccatagtg aagagttgtg gagtccggtt cgttgcgctt ttacaggtgc attaacactc
    15541 tattactatt ttatcggctt ctttgggata cttgccgcga tagctataac gcttgtctgg
    15601 caaaggcata cgcgtttttt tcattagtcg taaatgcaga tactaaagaa cttaactaaa
    15661 cttcacatcg ccgcttcatt tttcgcccgt agcttcttcg cttggctttc cagtagcagg
    15721 taacagatca gcgccagcag cagcgggata aagtaataca ggacgcgata agcgagcagg
    15781 gcggcgataa tgctcccttt cgaggtatgc tccccagcca gtagtgcgat aaacaccgct
    15841 tccagcacac cgatccccgc cggaatatgc acgatgacgc cagcaatact actaaccagt
    15901 aacacgccca gtacaaagaa atagttcacg ctttgaccaa gtaacagcca gataatcgcc
    15961 cccattacca tccagttaac gctggaaatc agcatctgag cgagggcgaa tttccatgaa
    16021 ggcagcacca gcttttgccc tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac
    16081 cacaaataaa ccgcgataat catcagtaag ccaatgccga gaatacgcag cgtagtttga
    16141 tcgacatacc agtaatccgg caactccacc acgcctgcgg taaagataat ccctgccagt
    16201 aaaatatagc ccagccagtt ggtggtaata ctgagcgagg aaatccgcgt aatagtgctg
    16261 cccggtaacc ctagtcggga gtacaaacga tagcgcatac caatgccgcc gacccaggta
    16321 ctgagcgtca ggttgaaggc gtagcagata aacgacacca gcatcacctg gcgcttcgcc
    16381 agtttgtgac cgcagtaaaa gcgggcgagc aggtcatagc agccgtaaat cagatagctg
    16441 acgaccacca gcccgaccgc actaagcagc gcaacgcgat tgtagtcgcg gatgaccttc
    16501 cagacctctt cccagtccac ttttttggcg tagaccacca gcaacacaat caccgcgata
    16561 aaaaacagcc aggtgaggat cttctttgct aagcgccagc gcgggtgtga tttactcatc
    16621 aggggtttac ccccgtgttt tcagtttcta cccgatcctg cgtttccatt gtcggttgtg
    16681 cggggggatc aacctgcgtc agacgtggtg tgtgtgccgg aagccagcca accagcgccg
    16741 ggaagtggcg taaaaagtgg aacgccagca cgcttttggt caggttccac caggtgcgtt
    16801 tgggcagcat ggtttcatcc acctgctgac aatctgcggc aataatgccg ttcagattat
    16861 cgcgcagcgt ctggttaaaa tgacgatcgt ggatgatgac atttgcttcg agattcagtg
    16921 acaaactgag cggatcgaga ttactggacc ctactgtcgc ccagtgatcg tccatcaatg
    16981 ccactttgcc gtggagcggg cggcggcggt actcaaaaac ctgaacgccg cctttaacca
    17041 gatagttata cagcaagcgc gcaccgactc tgacaatcgg catatccggt tcgccctgaa
    17101 tgatcagttt gatccgcacc ccgcgccgtg ccgctttacg caaggcgtgt aaaaatcgat
    17161 agccggggaa gaagtaggcg ttggcgataa tcacttcccg ctgcgcctga gtgagcattt
    17221 tcagataatg gcgttcaata tcatcgcgat gttcttcgtt atcgcgccag accagcaata
    17281 cctgcgcttc tcccggctgg cggttctctt ccgctttgtg atgacgtcgc caccagcgtc
    17341 gtgccgcgct ctgtccaggc aggttttcca gctcaaactg gagaatatct tcgacaatcg
    17401 gcccttcaag gcgtaccgcg taatcctgtt tagcctctgg accgtagctg gacatatgct
    17461 cggcggagta attcagcccg ccaataaagg cgatacgcgc gtcgatcacc acaattttgc
    17521 gatgcatccg gcgaaacaca ttggtgcgca taccaaaaag gcgagggcgg ggatcgtagt
    17581 agcggaacac tacgccagct gccgtcagtt cattgacaaa ctcatcgctg agatccggcg
    17641 aaccgtagcc atccagcaag acttccgctt taaccccgcg ttgcgctgct gccagtagtg
    17701 ccgcatgcag ctgtttgccg acgttatcct caaaccagat aaacgtttca agaatgatgc
    17761 gttcttgtgc ctcgccaatc gccttaaaca ccgcgggata atattgctcg ccgttttcca
    17821 gcaactggat cttattgcct tcgcgccagc tacatttcat aaatgaatct ccgcacttaa
    17881 aggggcatga tcagaaaggt gtcgccatgt ccgcagcggc aacgcggttg gcgcgctggc
    17941 gctggcattt ttgacgtaga tcctgtccag tcgtagtaga ggaaattgca ccggaaacgt
    18001 gcgcgccggg cgtccgtggg cgcgggtaaa aatctcatcc agtccggcct gcacttttaa
    18061 cggatgatta gctttttgcc gccagtcatt gaaatcaccc gccaccaata ccggttcgcc
    18121 gtccggtagc tcattcaccc attcggcgag catcgcaagc tgcgcctgac ggtgcgcctc
    18181 acgcaggccc agatgtacgc acatcacatg aatcgctttt ccggtcatcg gcggcacaat
    18241 gcggcagtag agcacgccgc gcttttccgc accatcgacc gaaacatcgc gattctcata
    18301 atgttcaatg ggataacgcg acagtacggc gttgccgtga tgcccttccg ggtatacggc
    18361 attgcgaccg taggcaaaat cgctccacat agtgtcggcg agaaactcgt agtgcgaggt
    18421 atcgggccag ttttccacat gcagcggatg aacttcgtgc gcgcccatca cttcctgcag
    18481 gcaaacaata tcggcgctga cggtacgcac ggcatcgcga agttccggca aaatgaagcg
    18541 tcggttaaac gcggtaaagc ctttgtgaat gttgatggtg agcaccttga acgaaaattg
    18601 ttgtgtttga tcgggcataa ttttcctgtc tttgcctctt atctcattga aatagtgtag
    18661 tcggcgtcac aaaaaggtgc ggtcttacgg aattttccgt aaagttcggt actctgagta
    18721 agtagagata aattcttcag gagagaagcc atgaagtggc aacaacgtgt tcgtgtcgca
    18781 acgggtctaa gttgctggca gattatgttg catttactgg tagtggcgct gctggtggtg
    18841 ggctggatga gtaagactct ggttcacgtc ggcgtgggat tatgcgcact gtattgtgtc
    18901 acggtagtga tgatgctggt gtttcagcgc caccccgagc aacgctggcg tgaggtggca
    18961 gacgtgctgg aagagctgac cacgacctgg tattttggcg cagcgctgat tgtgctgtgg
    19021 ctgttgtccc gcgtactgga aaacaacttt ttgctggcaa ttgcagggct ggcgatcctt
    19081 gctggcccgg cggtagtgtc tttgctggcg aaagataaga agttacatca ccttacgtct
    19141 aaacatcgcg tacgccgctg accctgtcgt ggccgttatc accagtagcg gccacaaact
    19201 attccacaca atatccagac tcgcatcctt caaataaatc tgcttggtaa tgtccgtaaa
    19261 gtggcgaata gggttaatcc acgtcagatt ttgcagccac actggcatgt tttccaccgg
    19321 cgaaacgtaa ccggaaagga gaatggcggg catcataaaa acaaacacgc cgataaacgc
    19381 ctgctgttgt gtcgagcaga gtgatgaaat caacagaccg aatcccacca gcgataaacc
    19441 gtaaatcacc atcgtaaagt agaacagcgc tagcgatccg gcgaagggga tttgatacgc
    19501 ccagatacca atcgccagca caatggtggc ctggaaggtg gcgacaatta acgccggtac
    19561 ggctttgccg atgaagatct gccaggtggt gagcggcgaa accagtagct gatcgagcgt
    19621 accttgttca cgttcgcggg cgacggaaag tgaagtgacg atcattacgc cgatagtggt
    19681 gatcatggcg atcagcgacg gcaccacaaa ccatttgtag tcgagattcg ggttatacca
    19741 gttgcgtacc accagctcgc tgttgttggg tttcggtttt ccttccagca gctcctgctg
    19801 ataatttttg acgatctgtt gcaggtagtt ggcggcaatt tgcgcactgt tggagttacg
    19861 cccgtcgagg atcaactgca acggcgcggt ctggaaggta tccagtttgc gcgagaagtc
    19921 agccgggaaa cgcaccagta gtaacgcctt ttgtgtgtcg atggtcgggc ggatctcctg
    19981 tgggcttttc agcagcagca catgtgtaaa ggcgctggcg cgggcaaaac gttgagtaag
    20041 ttccaccgaa tgctcgccgt tatcttcatc gtagatggcg atggtggcgt tagtcacttc
    20101 cagcgtggcg gcgaacggga acaggatcac ctgaattagc acgggtaaaa tcagaatcgc
    20161 gcgggtttgt ggttcgcgca gcaacgactg caactctttg cggattaacg tccataagcg
    20221 atgaaacatg ctcttctccc taatccagcc gacgtttggt tttcagccac gtcaggccga
    20281 taaacatcac cgccgaagcg atcaaaaaca gcacgtttac caccagcacc actggaatat
    20341 tcccggcgag gaacaggctt tgcagggtgc tgacgaaata acgagcggga ataatgtacg
    20401 tcaccgcgcg gatcaccgcg ggcatactgt cgatctgaaa aataaagccg gaaagcataa
    20461 tcgacggcag aaaagcggcg ttcagggcga cctgagcggc attgaactgg ttgcgggtaa
    20521 tcgtggaaat cagcagcccc attcccaggg tactgagtaa aaacaggctg gagataaaaa
    20581 acagaatcag cagcgaaccg cgatacggca cgccgagaat aaacactgac accagcatac
    20641 acagcaacat cgccagcatc ccgagaaagt aataagggat cagcttacac agcagcagtt
    20701 ccgtgcgggt aatctccgta gagagcagag cctccatggt gccgcgttcc cattctcgcg
    20761 ccaccaccag cgaggtgaga atcgcgccga tgaccgtcat gataatggtc accgcacccg
    20821 ggataataaa gtgctggctt atcgccgccg ggttaaacca gtagcgggtt tgtacatcaa
    20881 taagcggttc aaaagtctgc ccgttgtcct ccgctcgctg catttgccag atctgccaga
    20941 tcccttcgac atacccctgc acaaagttag cggtattcgg ctcactgccg tcggtgatca
    21001 cctgaatcgg tgcggtggcg ttggcgcgct ccatctgttc ggcgaaatcc acgggaataa
    21061 ccaccagacc gcgaattttc cccgactgca ttttggcgat cagttcctga cggttatcgc
    21121 tgatggtggc gtcgatgtag ggcgaaccgg tcatggtgtg ggtgaaatcc agcgccgctt
    21181 cgctacgctg ttccagcaaa atcccgaccc gcagcttgct ggagtcgagg ttaatgccgt
    21241 aaccaaaaat aaacagcagt agcagtggga tcactaccgc aatcagccag ctactcggat
    21301 cgcgaacgat ctgccgcgtc tctttgacgc acagcgcccg cacgcgatgc caggaaagga
    21361 tcgggttact cattgctatg ctccttatcc cagtcgtgga tcaactgaat aaaggcttgc
    21421 tccatggtgg gatcgggttg ctcatcgtta gctgactgtg ctttcaaatc gtccggcgta
    21481 ccgctggcga ttaatttccc acggtacacc aggccgatgc ggtcgcaata ttccgcttca
    21541 tccataaagt gggtggtgac catcaccgtg acgcctttct ctaccatgct gttgatgtgc
    21601 agccaaaatt cacggcgggt gagggggtca acgccggaag tcggttcgtc gagaaagaga
    21661 atatccggtt catgcatcag cgaacaggcc agcgccagcc gctgtttaaa acctaatggc
    21721 agttcatcgg tggcgtgtga ggcaatactt ttcaggccaa atgcctcgct catacgggag
    21781 attttttcgt tctgcgcccg accgcgtaag ccatacacac cagagaaaaa gcgtaaattc
    21841 tgttcgaccg tcaggttgcc gtagagcgaa aatttttgcg ccatatagcc gagatgctgg
    21901 cgcgctttac cggaactctc tttcagatcc atccccagca ccagcgcctg gccggaagtc
    21961 ggcaccagca aaccgcacat catcttaaag gtggtcgatt tacccgcgcc gtttggcccc
    22021 agcaaaccaa aaatctcccc acgtttaacg gcaaagttga cgtgatcggt ggcggcaaaa
    22081 tcaccaaatt tcttggtcag ttctttcgct tcgatcaccg tctcgccggg tgtgccttct
    22141 accgtatgca atattgcacc cagcggcgat tctgaggttc cggcaccgcc cagcaaatca
    22201 ataaacgcat cttcaaaacg cggcgtagtt tcgttgatgt tgatttccgg catcccgtcg
    22261 gcatggcgaa tatcgtctgg tgtggcctct tttttgagga tcagacgtac cgatttcccc
    22321 tgaatcatgc catcgctgac ctgcggcagt ttcaaggcgc gttgcaacag tttgcggttg
    22381 ccctcgtgtg gactggtcat cagaaagctg cgtccggcca tggtttgtgt cagggctttt
    22441 ggttctccct gatacagcaa ctcgccttcg ttcatcagta acacgtcacg gcactgctcg
    22501 gcttcgtcga gatacgaggt actccagagg attaacatcc cttcgcccgc cagctcatgc
    22561 accatctgcc acagttcgcg ccgtgagata gggtcaacgc cgacgccggg ttcatcgagc
    22621 agcaacactt tcggttcgcc caccagggta caggccaggc cgagtttttg tttcatccca
    22681 ccggagagct tgcccgccag gcgtccggta aacggcccaa gagacgtaaa ctccagcagg
    22741 cgagcaaaag tttgcttacg tgcctcgccg gtgacgctgc gcaaatccgc gtacagattg
    22801 aggttctcca tcaccgtgag atcttcatac agaccaaatt tctgcggcat atagccgagc
    22861 acggcgtgca gcgcgccgtc gtttttgatc ggatcaaagc caatcaccgt ggcactgccg
    22921 ctgtcgggtt tcagtaatcc cgccaacatc cgcatcagtg tggttttacc tgcaccgtcc
    22981 ggccccacca accccgtcac ataaccggcg tgaatggtac aatcgagcgg cgcgacggcg
    23041 ggcttgtcca tgcccggaaa gcgtttttcc aggccgttca gcgtgataac ggcatcattc
    23101 atgtcctgcc tcgtcaccga attgtaccgt cactggcatt ccctggcgta acgcatcatc
    23161 ggcgtcggtc accacaatac gcaggcgata gacgaggtcg gtacgcagat ccggtgtttc
    23221 gacggttttc ggggtaaatt cagcagtcgg cgaaacgaaa ccaatctgcc cgtgatacgg
    23281 cttgtccggg cgaccatcgg tataaagcag cactttgcgc cccggctggg cctggtcaag
    23341 attccgttca tcaacataag cgcgcaccca caccggacgc gttagtgaaa cggtaaacac
    23401 cgtgccacct tcattgagaa ccgtgcctgg ctctaccgcg cgcgttaaca gcgtgccatc
    23461 agacggtgct atcaacgttg agtcctgtaa attcagctcc gcctgcgcca gttgcgcctg
    23521 cgcctgttcg aggctggctt tcgcctgagc gatgtcctgt tcacggttac cggaacggta
    23581 ctgacgcaat ttatcctgtg ctgatttcag cgttgcctgt gcctggtcgc gcgaggagcg
    23641 ggcattttcc aggtcatttg ccgaaatggt gcggcttttc cacagtcctt gctggcggtt
    23701 atagaagttc tgcgcatagt cataggcggc ttgcgcctgt ttcaccgctg cggcggcctg
    23761 agcgatttct tcatcgcgat acccggcaag catcagatca tactgcgcct gtgccaccga
    23821 gacgcccgct ttcgcctgca tcagggcaat ctcatacggc ttgtgatcca gttcgcccag
    23881 cacctggccc gctttgatag catcaccttc gtccaccgcc agcgattcaa cgcgcccccc
    23941 aacacggaaa ctaagattca ccgtacgaat atccacgttg ccatacagcg tcaggccgtt
    24001 atcctggcgg ctttgatacc accagtagcc tccggcaacc acggcggcaa gtaccactac
    24061 cgccagtccg atcacgacag gttttttcat cactacaaac tcctttgcga taatccttgc
    24121 aaaatcaggt cgatatgaca ggtcaccgtc tggttgatca gttcggtctt ttcttcatcg
    24181 aacgcggtcc agccggtacg taacagaatt gtttctttgc caagacggaa cgccagaatc
    24241 tcaccaatca acgcatgggt atgaaggatc atgcgggtgt cattagcgtc gcagccggtc
    24301 caggcggcaa tcaggcgtgt caggtggctg tgtagcggac taatcacctg ttcgtgcacc
    24361 aggtggtagg ctgccgtggg agagagctgc tcacgggaga taaacttgct gaggttgacg
    24421 gtgtcatcct gggtgagcag cttaatcatg ttcctgcagg cgcgaaggat cagttcacgg
    24481 atggcagccc gatcaggctg tggttgggcg aacaagcgtt cggcttcctc ggcatgcgga
    24541 cggaactgct cgccaataaa atcggcaatc cactgggcgc aggcgaggta caaatcttct
    24601 ttcgaaccga agtagtaggt gatggcagcg atattctgcc cggcctgggc ggctatctcg
    24661 cgggtggtgg cgttcattcc atattcacca aactgcgcca gtgcggcagc aatcagctgt
    24721 tttttcgcct gttcaccctt gattgtcatg gcaggattat tcatcgcaca gtccattctt
    24781 aatcaaatga ttgattaaga ttatgactcc atatgggagt tgtccagtat ggctaagaat
    24841 tttagctacg ccagtcacag ggataattta tgcgctgcgt cacaaaaact gctacactcc
    24901 gctccctcat gacattgtgg tttttgtcat tttccttttc agtatctccc tgaaaactac
    24961 accggtaacg gtcggggcgg ttcggagtag ttatgtcttt cgattctttg ggtttaagcc
    25021 ctgatatcct gcgcgccgtt gccgagcagg gttaccgtga acccacccct attcagcagc
    25081 aggcgatccc tgctgtgctg gaaggccgcg acctgatggc tagcgcccag accggcaccg
    25141 gcaaaacagc gggctttacg ctgccgctgt tgcaacacct gatcactcgc cagccgcacg
    25201 ccaaagggcg tcgtccggta cgtgcgctca ttcttacccc gacccgtgaa ctggcggcac
    25261 agattggcga aaacgtccgt gattacagca aatacctgaa cattcgttcg ctggtggtgt
    25321 ttggcggtgt cagcattaac ccacaaatga tgaagttgcg cggcggcgtt gatgtgctgg
    25381 tggcaacgcc gggacgtttg ctggacctgg aacatcaaaa tgcagtgaag ctggatcagg
    25441 ttgaaatcct cgtcctcgat gaagctgacc gcatgctcga catgggcttt atccacgata
    25501 tccgtcgcgt gttaacaaaa ctgccagcga aacgccagaa cctgctgttc tctgcgacct
    25561 tctctgacga tattaaagcc ctggcggaaa aactgttgca caacccgctg gaaatcgaag
    25621 tggcacgccg caataccgcg tctgatcagg tgacccagca cgttcacttt gtcgataaga
    25681 aacgcaaacg cgaattgctg tcgcacatga ttggtaaagg gaactggcag caggtgctgg
    25741 tgtttacccg taccaaacac ggcgctaacc atctggctga gcagctcaat aaagatggca
    25801 tccgtagtgt ggcgatccac gggaataaat cgcaaggtgc gcgtacccgt gcgctggctg
    25861 attttaaatc gggtgacatt cgcgtactgg tggcgaccga tatcgctgcg cgcggtctgg
    25921 atatcgaaga gttgccgcac gtcgtcaact atgaactgcc aaacgtaccg gaagattatg
    25981 tccaccgtat cgggcgtacc ggtcgtgcgg ctgctaccgg tgaagcgttg tcgctggtgt
    26041 gtgttgatga acacaaactg ctgcgtgata tcgaaaaact gctgaaaaaa gagatcccgc
    26101 gcattgcgat tccgggctat gagccggacc cgtcaatcaa agccgaaccg atccagaacg
    26161 gtcgccagca acgtggcggc atcaggctga tgaacaaacg caaaactgcc tgatgcgcta
    26221 cgcttatcag gcctacgtga actctgcaat atattgaatt tgcatgcttt tgtaggccgg
    26281 ataaggcgtt tacgccgcat ccggcatttc acaacaagca cttgtcagca atttgagaac
    26341 acgggaaaaa atttatctgt tttaccgccc ataagccacc gagacgttac ttctctatag
    26401 ccaattgttc gcgcaactcc attaaaaggt agcccagtcg attcttgccc ttaccattac
    26461 caccgtctcc ccagtaagca tcgttttccg tatgctcaac cagttttgcg ggcgcggttg
    26521 ccagcaagag cacacgcagt tctgcatgct gttcgaattt agcgcgaagt gctttacgca
    26581 tcacctgttc tttaaccgac tcccagtttt tacgaagtgg cttggaacga ttacgcccca
    26641 tgcgagcggc gaccatgggg gaagaaaccc gacggatctc ttcgcggtat ttttcatcaa
    26701 ggaatttttg tgcctgaaaa tagtgttcgg aggtgggcca ggtattcccg tcaaccttga
    26761 tgggcctggc ggcaaagtta gaaaaatcac cgtagtcatc gctggtgctg tagaaattta
    26821 tgatggtgtc ttgcatgacg tgttggatcc tttgtgctcg aacgggcatt aaaccgcatt
    26881 atgttggtgg ttattgcgag ccgctttcca gaaacagaaa aaccattacc cctgaaaacc
    26941 gaaaaatgcc acaatattgg ctgtttatac agtatttcag gttttctcat ggcattaacc
    27001 gccgcgctta aagcgcaaat tgccgcctgg tataaggcgc ttcaggaaca gatccccgac
    27061 tttattcccc gtgcgccgca gcggcagatg attgcggacg tcgccaaaac gctggccgga
    27121 gaagaagggc ggcatctggc gattgaagcc cccaccggcg ttgggaaaac gctctcctat
    27181 ttgatccccg gcatcgccat tgcccgcgaa gagcaaaaaa cgctggtggt gagtaccgcc
    27241 aacgtggcgt tgcaggatca gatttacagc aaagatttac cgctgctgaa aaagatcatt
    27301 cccgatctta aattcactgc cgctttcggg cgtgggcgct acgtttgtcc acgtaatctg
    27361 acggcgctcg ccagcactga acccacgcaa caggatctgc tggcgtttct tgacgacgaa
    27421 ctgacgccaa ataatcagga agagcaaaaa cgttgtgcga agctgaaggg cgatctcgac
    27481 acttataaat gggatggtct gcgcgatcat acggatatcg ctatagatga tgatctctgg
    27541 cgtcgtttga gtaccgacaa agccagctgc ctcaaccgta actgttacta ctatcgtgaa
    27601 tgcccgtttt ttgttactcg tcgggagatt caggaagcgg aagtggtggt ggcaaaccat
    27661 gcgctggtga tggcggcgat ggaaagcgaa gccgtactgc ctgacccgaa aaatctgctg
    27721 ctggtgctgg acgaaggtca tcacctgcca gatgtggccc gggatgcgct ggagatgagc
    27781 gctgaaatca ccgtgccctg gtatcgacta cagctggact tgttcacgaa actggtcgct
    27841 acctgcatgg agcagtttcg cccgaagacc atcccaccgc tggcgatccc tgaacgtttg
    27901 aatgcgcatt gtgaagagtt gtatgagctt atcgcctcgt taaacaacat tctcaatctc
    27961 tacatgcctg ccgggcagga ggcagagcac cgttttgcga tgggcgaact gcctgatgaa
    28021 ttgctggaga tctgccagcg gctggcaaaa ctcaccgaga tgctgcgtgg tctggcggag
    28081 ttatttctta acgatttaag tgagaaaacc ggcagccacg atattgtgcg tctgcatcgg
    28141 ttgattttac agatgaaccg cgcgctgggg atgtttgagg tgcaaagcaa actctggcgg
    28201 ctggcgtctc tggcgcaatc ttccggtgcg ccggtgacca aatgggcgac gcgggaagag
    28261 cgcgaagggc agctacacct ctggtttcac tgcgtgggaa tacgtgtcag cgatcagctg
    28321 gaaaggctgc tgtggcgcag tattccgcac attattgtca cctccgcaac cttgcgttcg
    28381 ctgaatagtt tttcgcgttt gcaggagatg agtggtctga aagagaaagc gggcgaccgt
    28441 tttgtggcgc tggattcccc ctttaaccac tgcgaacagg gcaaaattgt tattccccgg
    28501 atgcgctttg agccttccat cgacaacgaa gagcaacaca ttgccgaaat ggcggccttt
    28561 ttccgtgaac aggtggagag caaaaaatat ctcggtatgt tggtactgtt tgccagcgga
    28621 cgggcgatgc agcgctttct cgactatgtg acggatttac gcctgatgtt gctggtgcag
    28681 ggcgatcagc cgcgttatcg cttagttgaa ctgcaccgca aacgcgttgc caacggtgag
    28741 cgtagcgtgc tggtgggctt acagtcattt gccgaagggc ttgatttgaa aggtgatctg
    28801 ctcagccagg tgcatatcca caaaatcgct tttccgccca tcgacagccc ggtggtgatc
    28861 accgaagggg aatggctgaa aagcctcaac cgctatccgt ttgaggtgca aagcctgccg
    28921 agcgcctcgt ttaacctgat tcagcaggtt gggcgactga ttcgaagcca cggttgctgg
    28981 ggcgaagtgg ttatctacga caaacgcttg ctgacaaaaa attatggcaa gcgactactg
    29041 gatgcattac cggtatttcc gatagagcaa ccggaagtcc ctgaaggtat agttaaaaag
    29101 aaagaaaaaa cgaaatcccc acgccgtcgg cggcgttaat gatgtgagtc aggtaaggag
    29161 tcgtaaatgg actatcgcaa aatcattaaa gagatcgggc gcgggaaaaa ccacgcgcgc
    29221 gatttagacc gggatactgc gcgcggtctg tatgctcata tgctcaatgg tgaagtccct
    29281 gaccttgagt tgggcggcgt attgattgcg ctgcgtatca aaggggaagg ggaagcagag
    29341 atgctcggct tttacgaagc catgcaaaat cacaccatca agctgacgcc gcctgcaggc
    29401 aagccgatgc cgattgtcat ccccagttac aacggcgcgc gtaaacaggc taacctgacg
    29461 ccattattgg cgattctgct gcataaactc ggttttcctg tggtggttca cggagttagc
    29521 gaagatccaa ctcgcgtgct gacagaaacc attttcgaac tgatgggtat tacaccaacg
    29581 ttgcacggcg ggcaggcgca ggcgaagctc gacgaacatc aaccggtgtt tatgcctgtc
    29641 ggcgcgtttt gcccgccgct ggaaaaacaa ctggcgatgc gctggcggat gggcgtgcgt
    29701 aacagtgcac ataccctggc gaaactggcg acgccatttg ccgaaggtga ggcactgtgt
    29761 ctttccagcg tttcgcatcc ggaatacatt ggacgcgtcg cgaagttctt tagcgatatc
    29821 ggcgggtggg cgttactaat gcatggtact gaaggcgaag tgtatgctaa tccgcagcgc
    29881 tgcccgcaga tcaatctcat tgaccgtgaa gggatgcggg tgctgtatga aaaacaggac
    29941 cctgctggta gcgagttact gccacaagca aaagatccgg aaaccacggc gcagtggact
    30001 gagcgttgcc tggctggcag cgaagcgatt cccgaatcgc tgaaaatcca gatggcttgc
    30061 tgcctggtgg ctacgggtga agcggcaact atcagcgatg gcctggcgcg cgttaatcag
    30121 gcattttaat ttttatctca gccggatgag ccacgtcatc cggcttttac cccaccgcaa
    30181 tatgaaattc ctgcatcttt attgaccttc ccgcgcccgg cgtgcagcat aaaaaaacaa
    30241 caaacacata acataaacag gagttaacca tggaaagtgg tcatcgcttt gatgctcaga
    30301 cgctgcacag ttttattcag gctgtatttc gtcagatggg tagcgaggaa caagaagcga
    30361 aattagttgc cgatcattta atcgcggcaa acctggcagg gcatgattct catggtattg
    30421 gcatgatccc aagctatgtg cgttcctgga gtcaggggca tctgcaaatt aaccatcatg
    30481 ccaaaatcgt taaagaggcg ggggcggcag tcacgctcga tggcgatcgc gcattcggtc
    30541 aggtcgcggc acacgaagcg atggcgctgg ggattgagaa agcgcatcag cacggtattg
    30601 ctgccgtggc gctacataac tcgcatcata tcggccgtat cggttactgg gcggagcagt
    30661 gttcagcggc ggggtttgtc tctatccact ttgttagcgt ggtcggtatt ccaatggtcg
    30721 cgccgttcca cggtcgcgac agccgctttg gcaccaatcc cttctgtgtg gttttccctc
    30781 gtaaagataa tttcccgctg ttgcttgatt acgccaccag cgccattgca tttggcaaaa
    30841 cccgcgtcgc ctggcataaa ggcgtccccg tgccgccagg ttgcctgatt gacgttaacg
    30901 gcgtgccgac gaccaatccg gcggtaatgc aggagtcgcc gttggggtcg ctgttgacct
    30961 ttgccgaaca taaaggctac gcccttgcag cgatgtgtga aattcttggc ggggcgcttt
    31021 ctggcggtaa aacgacgcat caggaaacgt tgcaaaccag ccccgatgcc attcttaact
    31081 gcatgaccac tatcatcatc aacccggaac tgttcggcgc gccggattgt aacgcgcaga
    31141 ccgaagcctt tgccgagtgg gtgaaagcct cgccgcatga tgacgataag ccgattttgc
    31201 taccgggcga gtgggaagtg aacacgcgtc gcgaacggca ggagcagggg attccattgg
    31261 atgcgggaag ctggcaggcc atttgtgatg cggcgcggca gattggtatg tcggaagaga
    31321 cgttgcaggc tttctgtcag cagttagcca gctaaaaaaa agcccgtcca gtggcggacg
    31381 ggcaaacaag ggtaacatag gatcaatgag ggttagagca tatgcgtctg tcggcaaaca
    31441 gacagggaaa tacttgtgct ggacgtagcg taaacgcctg atccgtccaa cggttcggat
    31501 tttgtaggcc tgataagacg cggtagcgtc gcatcaggca tctgttgccg gatgcggcat
    31561 aaacgcctta tccgtcctac gaattactta taaattaccg cagtaccgct taatttattg
    31621 ttgttggtgg cggaagtgat gctgtatcca ctggcaccgg ctgccgccgc tttctcagcc
    31681 agtttcgctt ccagggcatc gaggggggat gcgccatcgg cagaaaccac gccgatttta
    31741 ttcatgttct gtgcctggga tgccgttacc ggttccgccg cgaacacgcc aaatgacaga
    31801 gttgaaagag ccatagcagc aacaacagta ttgatagttt tcataattaa tctctcgcag
    31861 gtgatttttg tataagtgac gttgtttcgt cgatgtgatg agtatcacgt ttttttacga
    31921 gagataaaat cgaagagaat tgaccgcctt gttcaaataa attgattgat atctaacata
    31981 ctgataaacc atttatttgt tgtacttatg gacttgtttt atgccgcgtg gcagtcacag
    32041 cagcgttaaa ggcacggaat gccacgcaaa atacaaatta tactcgctat gcatctcgcc
    32101 agttgtagtt aacgtaactg tctgtctttc gaacctctgc gattatatcc tgtatacgct
    32161 ggtttttgta aatctttctc ctgctgacaa tgaatacata agcgcacgcc aggaatggct
    32221 tcccgacggg cttgcgggat gggggcaccg cactcttcac attcatacag gctttcgcca
    32281 cgcggaattt caccccgagc gcgggcaatc gcatcttcaa ttgtactgtt gatctgttcg
    32341 ttgacggcgt catcgttagc ccaagcggat gccatatcgt cctccccata tcaatgcttg
    32401 tacagttaag tgtagctaat atagggacga acctgggcag ttcaagcgtc agatctccga
    32461 ccattcccgc agcagattat gataaagatt gagcagcgac aggatctctt cattttcacc
    32521 atagcggctt ttcagcgact gaatattttt gtccagttca aacagcatgg cgcgcttttt
    32581 atcatcgcgg atcatcgact ggatccacat aaatgatgcc actcgtacgc cgcgggttac
    32641 gggtgtcacg caatgcaggc tgctggaggg atacaacacc agatcacctg ccgggagttt
    32701 tacccgatgt tgtccaaagg tgtcattaac gaccagttcg ccgccgtcgt agctttgtgg
    32761 atcgcttaaa aacagcgtgg cagaaaggtc agtgcgcatc cagccgtttt gtggatggct
    32821 gcgtactgcg ccatccacat ggaaaccata ggtttcattg ttctgatagc gattaaacag
    32881 cggcgtggaa agggtacgcg gcaaggcagc ggcaaagaac aatgcatgtt ggttaaccgc
    32941 gttcagcacc tcattttgca gtgcggcgta taacgcgctg cgggtgtcta cttgctgatt
    33001 gttcttaact tgtgcacctt gtgctccggt ggtgacgcgt ccatccaccc attcggcttg
    33061 ttccagttgt tcgcgaaaat gggcgacgtc ctgtggcgat aacacgccgg gaatgtggta
    33121 catcatacaa gttctccaaa aagtggggcc tgcgccccac atctgaatca gaaatgcata
    33181 ttggctgtga gcaagaaggt tcttggctcg cccgggtgat aacggtagcc gctcttgttg
    33241 attgaggcga cgtaatcggt atcaaacagg ttgtaaacgt ttagctggaa gtcgagattg
    33301 cgattaaccc gataccccag tttggcatcg gcgacccagt aaccttctgt aaatgctggc
    33361 gttcccaccg cgccgtctga acctttatgt atactgccga tatagcgtgc gcccgcgcca
    33421 acagagatat catcggttgc ctgatattgg ctccataagg tgaaggcgtg ctccggggta
    33481 tacggcagcg atgaggaacc atcctgggca acatctttgc cgtttttgat ggttgctttt
    33541 tgctgggtat agccgccaat cacctgccac gcgggagtga tattccctgc cacggatatc
    33601 tcatagcctt cgacgcgttt cttaccgtat tgcgagtaag tcccgtcatc attttgctca
    33661 acttcatttt cgatatcagt acggaacagc gcggcggtga gcaacaggcg tttatccaga
    33721 acctgccatt tggtgccaat ctcgctggtg ttggcttttt gcggtttaaa atcggtgcgg
    33781 ttggcactgt taccactgcc agactgcgca agggcgaagt tgttgccgcc cggaggctgc
    33841 tgggaaacgg catagttaat atagacattg ccgttttccg tcaggtggta cagcgccccg
    33901 gctttccagt tcaccagatt gcccgacttg gcggtgtcga cggtggtgac tggagaacct
    33961 tttgccacac cagctgggca ggtgatggca ccgcgtccgc tgccgccgca ggcggtggca
    34021 ctgtcatatt cagtatgata attatccaga cggatcccgc cgttcagctc aaaatcacgg
    34081 gtgatttgca gcgtatcgaa ggcgtaaatt gcgaaggtat ccgtctgacc attggcgttt
    34141 gcgccgttgc gcgtcaggcc gccgggatga attctgctgt caggatgata aatatttacc
    34201 gccggtaacg tcaccggatt aacgccatag ttagtctgcg tttcacgggt aaattccacg
    34261 ccggtactga catcatgacc gatagaaccg gtatagaacg tcgaggtcag gttggtctgg
    34321 ttggtgagaa ttttattact cacatctttg gtattcgccg tgcgtgacta ggtccagcta
    34381 ttcacatcgc tggtgggctg agtaatattc gacgccccgc ccataatcgc cgtcatcagg
    34441 taatcctgct ttacgcgcga ccaacgggta gtattgcgaa tggtggtgtt atcgttgatg
    34501 tcgtgctcaa aacgcatggt ggcggtgtcg gtggtcgaat cgtcgtaatc ggaatccgtg
    34561 ccgtaaaagt tatgagtatc aacttttccg gaatgattca gggccgccgc tcccgcagat
    34621 ggggcagaat agcccggcaa accgatggtc ggaatgccgc cgtctggcgt gttgtgctga
    34681 gtgacatgca gataattaag atacaaacga tttgctgtac caaggccaaa agcgacagaa
    34741 ggggcgacgc cgtaacgctc atttttgact ttgtcgcgtc cggcatcgtg ggttttttcg
    34801 cccatcacat tcaggcgcac cgcggtagta tcaccaatga cctgattgac gtccagcgtg
    34861 ccgcggcgga accaggcgct gccaatactg gcggaggcgt caatgccgga atcattgcgc
    34921 ggctgcttgc tgatcatatt gatcgagcct gtcggtgcgc tgcgcccgta gtcggtgccg
    34981 gacggccctt taatcacttc gacctgctcg gtattgaagg tgtcgcgcga gacgctgccg
    35041 atatcgcgaa taccatcaat ataaatactg ttagaggtat cggcaccacg catataaatg
    35101 gcgtcgccag tggtggagtt accgttctca cccgcaaaaa acgcacccac gccgg
//
LOCUS       DAJTRH010000032        34804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTRH010000032 DAJTRH010000000
VERSION     DAJTRH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..2098
                     /locus_tag="OO339_001741"
     CDS             <1..2098
                     /locus_tag="OO339_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5926403.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="OO339_001742"
     CDS             2095..3183
                     /locus_tag="OO339_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5926404.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO339_001743"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO339_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5926405.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="OO339_001744"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="OO339_001744"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5926406.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="OO339_001745"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="OO339_001745"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5926407.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="OO339_001746"
     CDS             8051..8602
                     /locus_tag="OO339_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5926408.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO339_001747"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO339_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5926409.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO339_001748"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO339_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5926410.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="OO339_001749"
     CDS             complement(11683..12681)
                     /locus_tag="OO339_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5926411.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="OO339_001750"
     CDS             complement(12760..13452)
                     /locus_tag="OO339_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5926412.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="OO339_001751"
     CDS             complement(13529..14032)
                     /locus_tag="OO339_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5926413.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="OO339_001752"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="OO339_001752"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5926414.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="OO339_001753"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="OO339_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5926415.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="OO339_001754"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="OO339_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5926416.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO339_001755"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO339_001755"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5926417.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO339_001756"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO339_001756"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5926418.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21341)
                     /locus_tag="OO339_001757"
     CDS             complement(20922..21341)
                     /locus_tag="OO339_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926419.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21319..21672)
                     /locus_tag="OO339_001758"
     CDS             complement(21319..21672)
                     /locus_tag="OO339_001758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926420.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21673..21993)
                     /locus_tag="OO339_001759"
     CDS             complement(21673..21993)
                     /locus_tag="OO339_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926421.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22127..23560)
                     /locus_tag="OO339_001760"
     CDS             complement(22127..23560)
                     /locus_tag="OO339_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5926422.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="OO339_001761"
     CDS             complement(23623..24072)
                     /locus_tag="OO339_001761"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5926423.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO339_001762"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO339_001762"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5926424.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO339_001763"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO339_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5926425.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="OO339_001764"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="OO339_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO339_001765"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO339_001765"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5926426.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="OO339_001766"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="OO339_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO339_001767"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO339_001767"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5926427.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="OO339_001768"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="OO339_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34082..34157)
                     /locus_tag="OO339_001769"
     tRNA            complement(34082..34157)
                     /locus_tag="OO339_001769"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="OO339_001770"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="OO339_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8488 a         8949 c         9148 g         8219 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa aacgtcaccg
    34801 gggg
//
LOCUS       DAJTRH010000033        34663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTRH010000033 DAJTRH010000000
VERSION     DAJTRH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            618..2903
                     /gene="nrdA"
                     /locus_tag="OO339_001771"
     CDS             618..2903
                     /gene="nrdA"
                     /locus_tag="OO339_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5926428.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3137..4267
                     /gene="nrdB"
                     /locus_tag="OO339_001772"
     CDS             3137..4267
                     /gene="nrdB"
                     /locus_tag="OO339_001772"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5926429.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4267..4521
                     /gene="yfaE"
                     /locus_tag="OO339_001773"
     CDS             4267..4521
                     /gene="yfaE"
                     /locus_tag="OO339_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5926430.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4575..5225)
                     /gene="inaA"
                     /locus_tag="OO339_001774"
     CDS             complement(4575..5225)
                     /gene="inaA"
                     /locus_tag="OO339_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5926431.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5404..5646
                     /locus_tag="OO339_001775"
                     /pseudo
     CDS             5404..5646
                     /locus_tag="OO339_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5688..6764)
                     /gene="glpQ"
                     /locus_tag="OO339_001776"
     CDS             complement(5688..6764)
                     /gene="glpQ"
                     /locus_tag="OO339_001776"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5926432.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6769..8127)
                     /gene="glpT"
                     /locus_tag="OO339_001777"
     CDS             complement(6769..8127)
                     /gene="glpT"
                     /locus_tag="OO339_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5926433.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8400..10028
                     /gene="glpA"
                     /locus_tag="OO339_001778"
     CDS             8400..10028
                     /gene="glpA"
                     /locus_tag="OO339_001778"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5926434.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10018..11277
                     /gene="glpB"
                     /locus_tag="OO339_001779"
     CDS             10018..11277
                     /gene="glpB"
                     /locus_tag="OO339_001779"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5926435.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11274..12464
                     /gene="glpC"
                     /locus_tag="OO339_001780"
     CDS             11274..12464
                     /gene="glpC"
                     /locus_tag="OO339_001780"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5926436.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12657..13607
                     /locus_tag="OO339_001781"
     CDS             12657..13607
                     /locus_tag="OO339_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5926437.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13648..14118)
                     /locus_tag="OO339_001782"
     CDS             complement(13648..14118)
                     /locus_tag="OO339_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5926438.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14173..15375)
                     /locus_tag="OO339_001783"
     CDS             complement(14173..15375)
                     /locus_tag="OO339_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5926439.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15462..16004)
                     /locus_tag="OO339_001784"
     CDS             complement(15462..16004)
                     /locus_tag="OO339_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926440.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16283..16708
                     /gene="nudI"
                     /locus_tag="OO339_001785"
     CDS             16283..16708
                     /gene="nudI"
                     /locus_tag="OO339_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5926441.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16747..17349)
                     /locus_tag="OO339_001786"
     CDS             complement(16747..17349)
                     /locus_tag="OO339_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5926442.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17657..18796
                     /gene="arnB"
                     /locus_tag="OO339_001787"
     CDS             17657..18796
                     /gene="arnB"
                     /locus_tag="OO339_001787"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5926443.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18800..19768
                     /gene="arnC"
                     /locus_tag="OO339_001788"
     CDS             18800..19768
                     /gene="arnC"
                     /locus_tag="OO339_001788"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5926444.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19768..21749
                     /gene="arnA"
                     /locus_tag="OO339_001789"
                     /pseudo
     CDS             19768..21749
                     /gene="arnA"
                     /locus_tag="OO339_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21746..22636
                     /locus_tag="OO339_001790"
     CDS             21746..22636
                     /locus_tag="OO339_001790"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5926445.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22636..24288
                     /gene="arnT"
                     /locus_tag="OO339_001791"
     CDS             22636..24288
                     /gene="arnT"
                     /locus_tag="OO339_001791"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5926446.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24285..24620
                     /gene="arnE"
                     /locus_tag="OO339_001792"
     CDS             24285..24620
                     /gene="arnE"
                     /locus_tag="OO339_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5926447.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24620..25006
                     /gene="arnF"
                     /locus_tag="OO339_001793"
     CDS             24620..25006
                     /gene="arnF"
                     /locus_tag="OO339_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5926448.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25000..25272)
                     /gene="pmrD"
                     /locus_tag="OO339_001794"
     CDS             complement(25000..25272)
                     /gene="pmrD"
                     /locus_tag="OO339_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5926449.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25377..26732)
                     /gene="menE"
                     /locus_tag="OO339_001795"
     CDS             complement(25377..26732)
                     /gene="menE"
                     /locus_tag="OO339_001795"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5926450.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26729..27691)
                     /gene="menC"
                     /locus_tag="OO339_001796"
     CDS             complement(26729..27691)
                     /gene="menC"
                     /locus_tag="OO339_001796"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5926451.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27691..28548)
                     /gene="menB"
                     /locus_tag="OO339_001797"
     CDS             complement(27691..28548)
                     /gene="menB"
                     /locus_tag="OO339_001797"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5926452.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28563..29321)
                     /gene="menH"
                     /locus_tag="OO339_001798"
     CDS             complement(28563..29321)
                     /gene="menH"
                     /locus_tag="OO339_001798"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5926453.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29318..30988)
                     /gene="menD"
                     /locus_tag="OO339_001799"
     CDS             complement(29318..30988)
                     /gene="menD"
                     /locus_tag="OO339_001799"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5926454.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31077..32372)
                     /gene="menF"
                     /locus_tag="OO339_001800"
     CDS             complement(31077..32372)
                     /gene="menF"
                     /locus_tag="OO339_001800"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5926455.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32451..32756)
                     /gene="elaB"
                     /locus_tag="OO339_001801"
     CDS             complement(32451..32756)
                     /gene="elaB"
                     /locus_tag="OO339_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5926456.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32811..33272)
                     /locus_tag="OO339_001802"
     CDS             complement(32811..33272)
                     /locus_tag="OO339_001802"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5926457.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33337..34254
                     /gene="rbn"
                     /locus_tag="OO339_001803"
     CDS             33337..34254
                     /gene="rbn"
                     /locus_tag="OO339_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5926458.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34445..34633
                     /locus_tag="OO339_001804"
                     /pseudo
     CDS             34445..34633
                     /locus_tag="OO339_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8462 a         9292 c         8600 g         8309 t
ORIGIN      
        1 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
       61 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      121 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      181 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      241 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      301 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      361 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      421 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      481 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      541 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      601 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      661 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      721 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
      781 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
      841 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
      901 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
      961 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1021 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1081 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1141 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1201 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1261 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1321 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1381 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1441 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1501 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1561 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1621 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1681 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1741 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     1801 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     1861 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     1921 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     1981 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2041 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2101 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2161 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2221 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2281 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2341 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2401 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2461 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2521 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2581 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2641 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2701 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     2761 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     2821 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     2881 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     2941 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3001 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3061 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3121 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3181 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3241 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3301 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3361 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3421 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3481 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3541 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3601 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3661 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3721 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     3781 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     3841 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     3901 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     3961 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4021 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4081 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4141 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4201 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4261 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4321 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4381 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4441 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4501 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4561 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4621 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4681 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4741 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     4801 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     4861 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     4921 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     4981 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5041 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5101 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5161 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5221 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5281 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5341 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5401 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5461 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5521 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5581 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5641 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5701 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     5761 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     5821 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     5881 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     5941 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6001 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6061 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6121 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6181 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6241 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6301 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6361 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6421 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6481 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6541 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6601 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6661 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6721 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     6781 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     6841 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     6901 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     6961 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7021 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7081 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7141 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7201 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7261 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7321 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7381 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7441 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7501 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7561 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7621 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7681 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7741 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     7801 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     7861 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     7921 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     7981 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8041 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8101 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8161 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8221 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8281 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8341 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8401 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8461 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8521 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8581 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8641 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8701 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     8761 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     8821 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     8881 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     8941 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9001 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9061 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9121 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9181 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9241 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9301 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9361 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9421 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9481 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9541 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9601 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9661 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9721 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
     9781 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
     9841 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
     9901 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
     9961 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10021 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10081 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10141 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10201 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10261 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10321 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10381 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10441 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10501 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10561 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10621 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10681 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10741 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    10801 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    10861 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    10921 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    10981 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11041 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11101 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11161 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11221 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11281 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11341 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11401 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11461 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11521 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11581 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11641 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11701 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    11761 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    11821 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    11881 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    11941 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12001 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12061 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12121 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12181 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12241 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12301 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12361 atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc
    12421 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12481 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12541 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12601 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12661 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12721 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    12781 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    12841 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    12901 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    12961 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13021 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13081 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13141 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13201 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13261 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13321 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13381 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13441 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13501 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13561 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13621 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13681 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13741 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    13801 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    13861 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    13921 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    13981 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14041 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14101 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14161 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14221 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14281 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14341 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14401 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14461 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14521 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14581 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14641 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14701 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    14761 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    14821 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    14881 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    14941 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15001 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15061 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15121 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15181 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15241 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15301 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15361 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15421 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15481 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15541 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15601 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15661 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15721 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    15781 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    15841 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    15901 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    15961 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16021 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16081 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16141 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16201 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16261 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16321 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16381 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16441 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16501 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16561 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16621 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16681 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16741 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    16801 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    16861 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa
    16921 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    16981 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17041 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17101 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17161 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17221 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17281 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17341 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17401 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17461 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17521 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17581 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17641 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17701 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    17761 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    17821 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    17881 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    17941 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18001 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18061 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18121 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18181 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18241 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18301 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18361 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18421 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18481 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18541 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18601 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18661 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18721 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    18781 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    18841 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    18901 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    18961 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19021 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19081 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19141 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19201 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19261 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19321 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19381 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19441 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19501 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19561 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19621 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19681 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19741 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    19801 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    19861 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    19921 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    19981 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20041 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20101 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20161 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20221 ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc gcgcgccagc
    20281 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20341 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20401 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20461 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20521 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20581 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20641 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20701 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    20761 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    20821 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    20881 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    20941 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21001 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21061 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21121 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21181 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21241 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21301 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21361 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21421 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21481 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21541 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21601 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21661 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21721 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    21781 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    21841 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    21901 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    21961 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22021 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22081 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22141 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22201 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22261 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22321 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22381 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22441 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22501 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22561 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22621 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22681 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22741 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    22801 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    22861 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    22921 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    22981 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23041 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23101 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23161 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23221 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23281 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23341 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23401 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23461 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23521 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23581 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23641 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23701 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    23761 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    23821 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    23881 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    23941 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24001 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24061 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24121 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24181 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24241 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24301 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24361 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24421 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24481 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24541 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24601 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24661 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24721 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    24781 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    24841 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    24901 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    24961 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25021 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25081 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25141 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25201 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25261 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25321 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25381 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25441 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25501 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25561 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25621 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25681 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25741 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    25801 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    25861 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    25921 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    25981 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26041 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26101 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26161 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26221 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26281 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26341 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26401 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26461 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26521 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26581 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26641 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26701 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    26761 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    26821 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    26881 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    26941 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27001 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27061 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27121 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27181 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27241 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27301 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27361 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27421 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27481 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27541 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27601 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27661 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27721 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    27781 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    27841 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    27901 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    27961 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28021 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28081 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28141 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28201 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28261 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28321 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28381 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28441 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28501 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28561 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28621 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28681 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28741 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    28801 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    28861 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    28921 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    28981 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29041 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29101 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29161 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29221 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29281 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29341 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29401 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29461 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29521 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29581 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29641 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29701 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    29761 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    29821 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    29881 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    29941 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30001 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30061 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30121 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30181 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30241 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30301 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30361 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30421 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30481 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30541 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30601 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30661 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30721 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    30781 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    30841 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    30901 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    30961 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31021 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31081 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31141 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31201 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31261 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31321 tcgcgcggta agccagcaac tgctgccgtt ggcagcaact gatgtaaaca gatcacatca
    31381 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31441 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31501 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31561 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31621 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31681 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31741 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    31801 actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    31861 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    31921 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    31981 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32041 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32101 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32161 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32221 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32281 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32341 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32401 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32461 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32521 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32581 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32641 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32701 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    32761 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    32821 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    32881 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    32941 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33001 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33061 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33121 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33181 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33241 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33301 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33361 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33421 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33481 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33541 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33601 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33661 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33721 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    33781 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    33841 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    33901 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    33961 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34021 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34081 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34141 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34201 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34261 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34321 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34381 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34441 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34501 atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt
    34561 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    34621 tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DAJTRH010000034        34445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTRH010000034 DAJTRH010000000
VERSION     DAJTRH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..332)
                     /locus_tag="OO339_001805"
     CDS             complement(<1..332)
                     /locus_tag="OO339_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926459.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OO339_001806"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OO339_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5926460.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OO339_001807"
     CDS             complement(1693..2073)
                     /locus_tag="OO339_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5926461.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OO339_001808"
     CDS             complement(2087..2503)
                     /locus_tag="OO339_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926462.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO339_001809"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO339_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5926463.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OO339_001810"
     CDS             complement(3504..3971)
                     /locus_tag="OO339_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5926464.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OO339_001811"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OO339_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5926465.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OO339_001812"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OO339_001812"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5926466.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OO339_001813"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OO339_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5926467.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO339_001814"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO339_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5926468.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OO339_001815"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OO339_001815"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5926469.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OO339_001816"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OO339_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5926470.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OO339_001817"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OO339_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5926471.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO339_001818"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO339_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5926472.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OO339_001819"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OO339_001819"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5926473.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OO339_001820"
     CDS             complement(14626..15441)
                     /locus_tag="OO339_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5926474.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OO339_001821"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OO339_001821"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5926475.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OO339_001822"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OO339_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5926476.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OO339_001823"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OO339_001823"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5926477.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO339_001824"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO339_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5926478.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OO339_001825"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OO339_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5926479.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OO339_001826"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OO339_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5926480.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OO339_001827"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OO339_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5926481.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="OO339_001828"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="OO339_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5926482.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="OO339_001829"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="OO339_001829"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5926483.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO339_001830"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO339_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5926484.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="OO339_001831"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="OO339_001831"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5926485.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="OO339_001832"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="OO339_001832"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5926486.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO339_001833"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO339_001833"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5926487.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO339_001834"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO339_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5926488.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="OO339_001835"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="OO339_001835"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5926489.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="OO339_001836"
     CDS             31575..32189
                     /locus_tag="OO339_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5926490.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="OO339_001837"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="OO339_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5926491.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="OO339_001838"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="OO339_001838"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5926492.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8698 c         9302 g         8337 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gtcacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcatcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 atttcctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cttggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ctctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgta acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgataatt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgtat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gcgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtaa tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgcag ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ttgtagcggg aaagcgtatt atgaacaccc
    34441 cgcgc
//
LOCUS       DAJTRH010000035        33677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTRH010000035 DAJTRH010000000
VERSION     DAJTRH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..891)
                     /locus_tag="OO339_001839"
     CDS             complement(<1..891)
                     /locus_tag="OO339_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5926493.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATF"
     gene            1137..1763
                     /gene="gstB"
                     /locus_tag="OO339_001840"
     CDS             1137..1763
                     /gene="gstB"
                     /locus_tag="OO339_001840"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5926494.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1760..2874)
                     /gene="yliI"
                     /locus_tag="OO339_001841"
                     /pseudo
     CDS             complement(1760..2874)
                     /gene="yliI"
                     /locus_tag="OO339_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2985..3368)
                     /gene="bssR"
                     /locus_tag="OO339_001842"
     CDS             complement(2985..3368)
                     /gene="bssR"
                     /locus_tag="OO339_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5926495.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3581..4906
                     /gene="rimO"
                     /locus_tag="OO339_001843"
     CDS             3581..4906
                     /gene="rimO"
                     /locus_tag="OO339_001843"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5926496.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4953..6281)
                     /locus_tag="OO339_001844"
     CDS             complement(4953..6281)
                     /locus_tag="OO339_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5926497.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6289..8631)
                     /locus_tag="OO339_001845"
                     /pseudo
     CDS             complement(6289..8631)
                     /locus_tag="OO339_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8809..9720)
                     /gene="gsiD"
                     /locus_tag="OO339_001846"
     CDS             complement(8809..9720)
                     /gene="gsiD"
                     /locus_tag="OO339_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5926498.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9723..10643)
                     /gene="gsiC"
                     /locus_tag="OO339_001847"
     CDS             complement(9723..10643)
                     /gene="gsiC"
                     /locus_tag="OO339_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5926499.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10661..12199)
                     /gene="gsiB"
                     /locus_tag="OO339_001848"
     CDS             complement(10661..12199)
                     /gene="gsiB"
                     /locus_tag="OO339_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5926500.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12219..14090)
                     /gene="gsiA"
                     /locus_tag="OO339_001849"
     CDS             complement(12219..14090)
                     /gene="gsiA"
                     /locus_tag="OO339_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5926501.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14077..15042)
                     /gene="iaaA"
                     /locus_tag="OO339_001850"
     CDS             complement(14077..15042)
                     /gene="iaaA"
                     /locus_tag="OO339_001850"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5926502.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15246..16481
                     /gene="moeA"
                     /locus_tag="OO339_001851"
     CDS             15246..16481
                     /gene="moeA"
                     /locus_tag="OO339_001851"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5926503.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16481..17230
                     /gene="moeB"
                     /locus_tag="OO339_001852"
     CDS             16481..17230
                     /gene="moeB"
                     /locus_tag="OO339_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5926504.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17409..18071)
                     /gene="fsa"
                     /locus_tag="OO339_001853"
     CDS             complement(17409..18071)
                     /gene="fsa"
                     /locus_tag="OO339_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5926505.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18202..19101
                     /locus_tag="OO339_001854"
     CDS             18202..19101
                     /locus_tag="OO339_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5926506.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19107..21539
                     /locus_tag="OO339_001855"
     CDS             19107..21539
                     /locus_tag="OO339_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5926507.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21685..22500
                     /gene="ybiV"
                     /locus_tag="OO339_001856"
     CDS             21685..22500
                     /gene="ybiV"
                     /locus_tag="OO339_001856"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5926508.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22652..23917
                     /locus_tag="OO339_001857"
     CDS             22652..23917
                     /locus_tag="OO339_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5926509.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24158..25750)
                     /locus_tag="OO339_001858"
     CDS             complement(24158..25750)
                     /locus_tag="OO339_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5926510.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25969..26889
                     /gene="ldtB"
                     /locus_tag="OO339_001859"
     CDS             25969..26889
                     /gene="ldtB"
                     /locus_tag="OO339_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5926511.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26947..28065)
                     /locus_tag="OO339_001860"
     CDS             complement(26947..28065)
                     /locus_tag="OO339_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5926512.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28062..28529)
                     /gene="mntR"
                     /locus_tag="OO339_001861"
     CDS             complement(28062..28529)
                     /gene="mntR"
                     /locus_tag="OO339_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5926513.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28715..28843
                     /gene="mntS"
                     /locus_tag="OO339_001862"
     CDS             28715..28843
                     /gene="mntS"
                     /locus_tag="OO339_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5926514.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29115..30698
                     /gene="opgE"
                     /locus_tag="OO339_001863"
     CDS             29115..30698
                     /gene="opgE"
                     /locus_tag="OO339_001863"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5926515.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30746..31261)
                     /gene="ompX"
                     /locus_tag="OO339_001864"
     CDS             complement(30746..31261)
                     /gene="ompX"
                     /locus_tag="OO339_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5926516.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31314..31379)
                     /locus_tag="OO339_001865"
     CDS             complement(31314..31379)
                     /locus_tag="OO339_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926517.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31614..32501
                     /gene="rhtA"
                     /locus_tag="OO339_001866"
     CDS             31614..32501
                     /gene="rhtA"
                     /locus_tag="OO339_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5926518.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32800..33303
                     /gene="dps"
                     /locus_tag="OO339_001867"
                     /gene_synonym="pexB"
     CDS             32800..33303
                     /gene="dps"
                     /locus_tag="OO339_001867"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5926519.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         8472 a         8922 c         8105 g         8178 t
ORIGIN      
        1 aaaggtggca tcaggtttaa ttggcgtcac agtttcaaag aagcggaaac cccaggtcaa
       61 taatttctca gactcattaa aacggatacg gtcagttttc gcccccagca ctacggagat
      121 taaacgcata tcaccctggg tagccgaagc aaccagatta tatcccgcac ctgccgtggt
      181 tcctgtcttc atgccatcaa cattcagatt gctgctccac agcagacggt tacggttagg
      241 ctgacgaatt ttgttgaagg tgaactcttt ctctttatga atggcgtact cttccggcac
      301 atcgtggatc aatgctttac ccagcaatgc catatcgcgc gcggtactga actgccccgg
      361 cgcatccagg ccgtgcaccg tctggaaggt agtgttggtc agccccagtt ttttggcata
      421 accattcatc agaccaataa aggactcctg gctcccggca acgtaatctg ccagcgcaat
      481 acaggcgtca ttaccggact ggataatcac acctttgttc aagtctgcca ccgaaacctg
      541 atcgcccggt ttgaggaaca ttaccgatga accacgcagt gccggatttc ccgtcgccca
      601 ggcatcttta ccgaccgtca ccatatcggt gagtttaatc ttatcggcct taagcgcctg
      661 cccaaccaca tagctggtca tgattttagt caggctcgca ggatccagtt tctcatccgc
      721 attgccttct gccagcactt taccgctggc gtaatccatt aaaatccatg cacgcgcatc
      781 cacgctcggc gcttcaacgg tttgttcggc cgcgaatgcc gttggggcaa aaaggaataa
      841 aaatgcagaa cccgctgcaa gaccacgaag gagagaggag tattgcgtca taaatgccac
      901 ccgagtatcc attcaaaaaa atacgttata tcgccgtatg ataagttcgg tgattaataa
      961 cgcacacaca aacttaaaga aaccgtaagt tggtaaagtt tttaaagttt atgcaatatc
     1021 ctgccgcttc gctgcttctg gtgtcatttt tttgccagct gttgcttaaa agttaacttt
     1081 acttcaccat cggagtcctg ttcagtctgt ggctgcctgt cggaaaaggg gtgagtatga
     1141 ttacgctgtg gggtcggaat aattcaacta acgtaaaaaa agtattgctg acgctcgaag
     1201 aactggaact accttatgag caaattctcg cgggccgtga gtttgggata aatcacgatg
     1261 ctgattttct ggcgatgaac cctaacgggc tggtgccgtt gttgcgtgac gacgaaagtg
     1321 atctcattct ttgggaatca aacgccattg tccgctatct ggcggcacag tacgggcaaa
     1381 aacgcctgtg gatcgactca ccggcacgtc gtgcggaagc ggaaaaatgg atggactggg
     1441 caaaccagac gctcagtaat gctcatcgcg ggatcctgat gggattagtc agaacaccac
     1501 cggaagagcg cgatcaggcc gccattgatg ccagttgcaa agagtgcgac gccctgtttg
     1561 ccctgctcga tgcggaactg gcaaaagtaa aatggttctc tggcgacgag tttggtgtgg
     1621 gcgatatcgc tatcgcaccg ttcatctaca atttgtttaa cgttggcctg acctggacac
     1681 cgcgtccaaa tctgcaacgc tggtatcagc aactcactga acgccccgca gtgcgcaaag
     1741 tggtgatgat tcccgttagc taattgcgtg ggctaacttt aagtaattcc ccactggact
     1801 cgtcggtgag aacgtataaa taaccgtcgg gtccagtgcg aacatcacga attcgctgcc
     1861 ctctgtccgt taaaatacgg ccatcttctg tcactttgtc gccgttgacg ctcatcacaa
     1921 tgacatcttt atctttcagc tcccaataaa taatttttgc tgccactggg ggaatttatc
     1981 gctgttatag aaggccatgc cgctcacagc gggcgaatct ttccagtaaa aaacaggttg
     2041 ctcggtcccg gcgacgatct cccctttcgc ttccggtatc ttaaagcctg aatagttgat
     2101 tccccaggtt gccagcggcc agccgtagtt tttgcctttt tgcgggatat taatttcatc
     2161 accaccgcgc gggccatgtt cattcagcca cagtgcatta ctccacggat tcatcgccat
     2221 tccttgcgga ttacgaatgc cataagacca gatctcggcg cgcgcaccgg attcctttat
     2281 aaaaggatta tcatccggga tttcgccctg gtcggtcaga cgcaccagtt tgccctgtaa
     2341 tttatccaga tcctgcgccg tcgggcgctg attgttttcg cccagagcaa taaaaagata
     2401 acctttaccg tcgaatacca gccgcccgcc aaaatggttg ccggtagaca gttttggcat
     2461 ctggcggaag acggtgcgga agtcggtcac ttttgagaga tcatcactta agcggccata
     2521 acccacagca gttccggctt tgccatcatc gccaacttcg gaataactta accagatgcg
     2581 gcgagactga gcaaaatcag gcgctaaaac cacgtccagc aggccgccct gcccgtgcgc
     2641 ccaaacgtcc ggaactccgg aaagcggcgc agataatcct tttcctgctt gccagtgacg
     2701 caactcgccg ccgcgcagag tgattaacat accgtgatta tcgggtaaaa aggccagtgc
     2761 ccagggatgg tcgagtttgt cttgcagtac ttcgacattt accgttgcag gagccgccca
     2821 gagagcggaa gaaagacaaa taaggggcac aaggaaaaag gattgtcgat gcataacgcc
     2881 tccggattga ctctggctta aagcgtagtc agtggaggag ataacaaatt catttttaca
     2941 aaaacttaaa catgaagggg gagacgcttt ctccccctta gttttcaggc cttctcaagc
     3001 atggcgtgct tctgcaggct ctggatactc agcgttaagc tcatcagaca attttcaagc
     3061 ttatcggcgt tgacggtaat aacagtcggg caatcatggt gcccactcat caaacatact
     3121 gcggctgtcg ctaatgcttc ttcagcatga tgaagagcac tccactcttc ctgatccaga
     3181 tgaagattca accgcagcga tttatcgtgc agttcgcgat tcagtttaaa aaagttatct
     3241 cgtagatgat tgctttcgct gacggacatg tatccttttg ccttcctcag ttgcacgtat
     3301 gcagcgaggt caacgcgtgc gtcgatcaac cggttcagca gatcgattcg ctgtctgtca
     3361 acgaacatac ttcgatcctc ctctttccag ccttgttgca attaaatagt atggctattt
     3421 tttgagcaag atgctgtcca ggatcaattc ttcacgccga attaacaaaa tttacatttc
     3481 cttcgcctcc gccagcgaat gagattgcga tacaaatttt atccagaact ctgtagaatc
     3541 cctgccctga cctttcctgt aactgaatat tttttaagct atgagcaaag taactcccca
     3601 gccgaaaatc ggctttgttt cccttggctg tccgaaaaac cttgtcgatt cagagcgtat
     3661 tctcaccgaa ctccgcactg aaggttatga cgtggtaccg agctatgacg atgcgaacat
     3721 ggtgatcgtc aacacctgcg gctttattga cagcgcggta caagaatcac tggaagccat
     3781 tggtgaagcg ttgaatgaaa acggcaaggt aattgtgacc ggttgtctgg gggcaaaaga
     3841 agatcagatc cgcgaagtcc acccgaaagt gctggaaatc accgggcctc atagctatga
     3901 gcaggttctg gagcacgttc atcactacgt gccaaaaccg aaacacaacc cattcctgag
     3961 cctggtgcca gaacaaggtg tgaaactgac gccgcgtcat tatgcctatc tgaaaatttc
     4021 ggaaggctgt aatcaccgct gcaccttctg cattattccg tctatgcgcg gcgacctgat
     4081 gagccgtccg attggcgaag tgttaagtga agcgaaacgt ctggtagatg cgggcgttaa
     4141 agagattctg gtgatctcgc aggatacttc cgcctatggc gttgatgtta aacatcgtac
     4201 tggcttccac aacggcgagc cggtaaaaac cagcatggtc agcctgtgcg aacagttatc
     4261 gaaactgggg atctggacac gtctgcacta cgtttaccct tatccgcatg tggacgacgt
     4321 catcccactg atggcagaag gcaaaatcct gccgtatctg gacattccgt tgcagcacgc
     4381 cagcccgcgc attctcaaac tgatgaagcg tccgggttct gtagatcgcc aactggcgcg
     4441 catcaaacag tggcgcgaaa tctgcccgga actgacccta cgctcaacct ttattgtcgg
     4501 cttccctggc gagacggaag aagatttcca gatgctactc gacttcctga aagaagcacg
     4561 tctggatcgc gttggctgct ttaaatacag cccggttgaa ggtgcagacg ccaatgccct
     4621 gcctgaccag gttccggaag aagtgaaaga agaacgctgg aaccgtttca tgcagctgca
     4681 gcagcagatt tccgccgagc gcctgcaaga gaaagtgggc cgtgaaattc tggtgattat
     4741 cgacgaagtg gacgaagaag gcgcgattgg tcgcagcatg gcagatgcac cggaaatcga
     4801 cggcgcggtt tatctcaatg gtgaaaccaa cgttaagccg ggtgatatcc tgcgtgtgaa
     4861 agtcgagcac gccgatgagt acgatttgtg gggtagccgg gtttaattaa ttcaggccgg
     4921 gcaaaatcat ttgtccggcc tgcgaataat gattactgcc agctaatctg tgccctgccg
     4981 tttttcttac tcttatacag ccgctcatcc gccagcattt tcaagcgttc gacggtagta
     5041 cattcatttg aatgcgctac accgatactg gtgcttaaat taatgcttcc cacctggaaa
     5101 tgaacgattt cttttagtat cgatagcaac tcttccagcg tgccgccagt ataaatcacc
     5161 gcaaattcgt cacctccgaa gcggtagact ttacctttat ctaccgggat ctgtgattta
     5221 aggattttca ccactttcat caacacttca tcgcccagca aatgaccgaa ggtgtcgtta
     5281 atgtttttga atttatcgac gtcaaacaga cacaaataac cgcctttttc cggcagcgct
     5341 tttaaatctt catcaaagga ttttctgccc attgctttgg tcaggtcgtc caggaacgcc
     5401 gagttataca tactgtagag ttttttgaca tcggcagccg aaaagaaaag cccgccatcc
     5461 agtacgcgtt caaaatggtt attaatgatg gcttctttct ccacaatacc acgattaatc
     5521 actctgcgta ccagatagag gcaactggca cccaatatca ctgccaccaa tgcggcaaag
     5581 aaaataatca atgtacaaac ccgcaaataa acatcttttt tatacgcaaa tacgtacaat
     5641 ccggtcggca aataggcgaa gtgctccatc gcttccgaaa cggtgaagat ggcgttttta
     5701 gcattatatt catcaccggt caccggttta ccgttcttaa atagagagaa catcaggttc
     5761 tcgctgtcca tcaccagcga ttttgacagt aaagaaaaac gctccagacc aagcaaaaat
     5821 atcagactac gattgtgagt taccggatag aggtaataga cgaaggtttt accgtcaaaa
     5881 gagataaacc ctgtattttt gacatgtcga tacttcacaa cctgcataat atctgcacaa
     5941 taatttttct cgaactcctt tacctgaata cattcgtcac tggaaatcag ataaggaccg
     6001 acatcgtaca aataagaggg ggaaaccgag tcggcgtaag ctgtgcgcaa accaaccaac
     6061 agctgttcct ccgtgggatt tagcgtgttg ataagatcaa ttgccacgct acgccgatat
     6121 tcagcaatat ttgccaccgc aaaagcagta tcaataacgg actgtttcag gctatacacc
     6181 cgttccttta ccatttgagt gtagatcccg caggcaactg ctgaaatcat gataaaaatc
     6241 agcagactga ctgtaagtac gaacttattg attctggaca tacgtaaact actcttttac
     6301 taattttcca cttttatccc aagcggagaa tggcatgggt ttctggtaca gatacccctg
     6361 agcctgaaca catcctaatt taattaattc gcgcgcaacg tcttcgctct ctacgccttc
     6421 cgcaatcacc gggatccggt tatagcgtgc cagatcgaaa ataatgcgca caatttttcc
     6481 cgcagtaccg aaaatatcgc cggtaaattg cgagtccacc tttatgctgt ctggattaat
     6541 ttcataaaaa cgtttcaggt ttgacaaacc ggtgccaaag tcatcaatcg atatccgtac
     6601 cccttgttta cgcagttgtt caatattgag ataaaaatgt tcattaattc gctcgatcac
     6661 ggtttcagtg atctcaacac agaggctgtt cgccttcagg ccattttctt tcatgatggt
     6721 ggtaaaacgc tcatgaaagt gcggttcgtt taactgccag ggcgaaacat taatatgcag
     6781 taagaaatca tccttcagcg acgcacgttg actaaaatgt ctgaattaac ccattgccgt
     6841 tttaataatc tgataaccca gctcattgat aaacccgata tcttcagcaa tggtaatgaa
     6901 cttcagcggt gaaatgatcc cccgctccgc agaaacccaa cggcacaacg cctccgcgct
     6961 cagaatggcg cgggtgttaa tatcaacgat cggttgatag acgaggaata gttcgccaat
     7021 ttccattgca tggcgaatat tacgcgcaat ccagatattc tcttctattt cgttgagttt
     7081 cgcgtcatca cagaaaataa actcggagaa catatggtca cggatactgg aaatcgccag
     7141 tttcagtttt tctgcgtaac tccaggcgtt ttccccctgc aaaggttcac aaatggcaga
     7201 cttatggatg cgcagattgc ggtagccaaa gctgctgtca aaatcgttta ccatcgctat
     7261 tttacgggta aatgtctgta cattttcccg acacagaagt aaatagagat cggcattgaa
     7321 tgtcacgata tcgcagcatt gcgcatattt ctcggcaatt cgctgacaca atttcctgac
     7381 gattttattg cgttcatcaa caccaaacac cgcctccacg gcttccagac cacacagttt
     7441 gatgatcccc acagccagat actgattatt gttggactca ataatcgcca tatttttaat
     7501 aaaaccgctt tcctgatcgt aagagatcag tgagagcaat ttatttgacc gttcaatttc
     7561 ggcatctttc atttccgcga tcgaattttt gatgctgata atctctttcg catcgccttc
     7621 gcgaattttc agagcctcga tctgatccgt tgagtcgggc aaaaacgcca gcgtattaac
     7681 cagctcggtc atttcagaaa attgccgacg gaacacacga gtggtcatga aatataccac
     7741 cagcaacacg ataataatga acgcaaacgg tagataaaaa agtttatctg atgcgttcaa
     7801 taaattaccg ccaggataat aaaaatagat ttcgaacaat gcattatttg gcactgctga
     7861 tgtctttgca atatactccg tatcgacaga atcaataata ttataattac cttcgctatt
     7921 ataaataatc agtttttcac gagcgagttg attcttggca atgatctcat gaccttgttt
     7981 caaaaataga acttcatcat tagcgctgat ttgatcaaga tactcgttca gataccgcca
     8041 gtttaaatcg acatgaattt tatctaccgc taacaaatcg tgatctttgc cgttgatacg
     8101 tagcgtaaaa gagatacatt ttttacccgg acgatcaaag caattgtatg ccggattcca
     8161 gctgttgtta gagtcatctg aagccaccga ccacggtcgt ttcgatgggt taaaatcggc
     8221 aaaaataacc cgccctgact cgtcgacaag cggtccattt acttgttcct gatgataaac
     8281 aacgatttta tcattcgccc tccgcgaaaa gagataatat ttattatcat caagtaccag
     8341 tcccaccgag tctaaaaatg gcatcatttc cattttcaga atcacggcgc gttgaatctc
     8401 ctctgtattt tttaataaat acagtgagtc ctttaatgtc agggccgaac gttcaatatc
     8461 tttaaagaaa tagccaacgt ttttctgaat aagttcaaag cggctatcgg cacgttttac
     8521 cgcgcgttcc gataccagtt gatagttaat catgaaaaaa agaccaataa atgacagtgc
     8581 tgccaataaa aataaaatta tcagccttat cttttcgtat aaactcagca tgttaataca
     8641 tttaacaaaa taactatctg aacgctgtgc cgctgcaaaa atagtccctt gctgcggctt
     8701 tttaaccaaa ttacccgatt ctacaaaatc agcagaaagt tacaacactt gcttagggca
     8761 ataatttcct gtgaggtgat taccctttca agcaatattc aaacgtaact atcctttaat
     8821 tttcggatcc agcgcatcgc gtaaaccatc gcccaacaaa ttgaacgcca gtacggtcag
     8881 aaaaatagcc agggccggaa aaacagcgac atgcggcgcg ataaccatat ccgctcgtgc
     8941 ctcattgagc attgctcccc actctggtgt cggcggctgc gcaccgaggc cgagaaatga
     9001 caggctggcg gcagagataa tcgaggtgcc aatgcgcatg gtgaaaaaca ccacgataga
     9061 agagacggtc ccaggcagga tatgacgcaa taaaatggtc atatcgctgg caccaatact
     9121 gcgtgctgac tcaataaagg tttgctgttt caacaccagc gtgttgccgc gcaccaggcg
     9181 ggcaaacgcg gggatggaaa aaatggcgac tgcgataatc acgttagcaa tgccgcttcc
     9241 caacaccgca acaacagcga tcgccagtaa aatacccggg aaggcaaaca gcacatcgca
     9301 aatgcgcatg atcagccgat cccaccagcc ttcataatat ccagccaaca agcccagcaa
     9361 cgtccctatc gccacaccga taaacacggc aaacacgccc gccgccagcg agatttgcgc
     9421 accaaccagg acacggctga aaatgtcgcg ccccagtgaa tcgacgccaa accagtgctg
     9481 caaagaaggt ccgttattca gattgtcata atcaaaataa ttttcggcgt catagggagc
     9541 gatccagcgt gcaaaaatgg ccaccacaat caataaaata acgaataatg cggcggtcat
     9601 cgccatatgc tgacggcgaa atcgtcgcca gaattcatgc cacggtgtac gtacctggtc
     9661 aggtttgacc agtggcatgg cgtttaacac cgcctgacgt cgccagttaa atagtcgcat
     9721 ccttacttgt acctgatagc cgggttaatg gcggcgtaaa gcacatccac cactaagttg
     9781 ataagaataa attccagcga gaaaagcaga atttccgcct gaatcaccgg gtaatcgcgc
     9841 atttctacag agtcaacgag taagcgtcca agtccgggcc agttgaaaac tttctccacc
     9901 acgatagaac caccgagcaa aaagccaaac tgtaagccca tcatggtcac taccgggatc
     9961 atcgcgttgc gtagcccgtg tttgaggaca acccaggttt cgctcacccc tttcgccctc
    10021 gcggtacgca tataatcttc gcttaaaacg tcgacaaatg acgcacgggt aaagcgcgcc
    10081 atcacggcgg ccactgccgc gccgagcgtc agggagggta aaatgtagtg ctgccaactg
    10141 tctgctccca cagtaggcag ccagcccagt tcaacggaga atacctgaat taaaaacatc
    10201 cccagcgcaa atgccggaaa cgagatcccc gacaccgcaa tggtcatact caatcgatcc
    10261 ggccaacgat tacgccagac ggcggcgata attcccgccg ccataccaaa tatcaccgcc
    10321 cagaccatac tggttatggt cagccatagc gttggcataa agcggctggc aatctcatcg
    10381 gcaaccggac gacgcgacac catcgacagg ccaaaatccc cctgcacagc attgctgata
    10441 tagtgccaga actggtgata cagcggctga tccaacccca gctgctgacg caccagttct
    10501 ataacctgcg catcagcttc gggcccggca atcaatcgcg ccggatcgcc gggcagcatg
    10561 tggacaaata aaaacaccag caccgagacg ataaacagcg tcggaatcaa ccccagtaat
    10621 cgtttgataa cgtaataaag cattccactc cctgcgttgc ttattgcaaa tccgcgtctt
    10681 caaagctgaa gccggtatct ggcatgatcc aaaaaccggt caggttttta ctgtgtgccg
    10741 acaccagttt ttctaccacc agcgggatcc acggcgattc ttgccagatg atatcctgcg
    10801 ccgccttata taagcgggtc ttttccgccg gatcattcgt tttcagtgcc tgagccagga
    10861 agtcatccac ctgtttattg ctgtaaaacg cggtattaaa cagcgtcggt ggccagtttt
    10921 gcgaggcaaa cagcggcgat agtgcccagt cggcttcgcc ggttgaagcc gaccagccag
    10981 tgtagaacat ccgcacgcca ctctcttttt gccctttacc ttcaacttct gccgcccgct
    11041 gtccggcatc catcgcggtc acctgggctt taatcccgac ctgcgctaac tgctgctggg
    11101 taaattgcag cactttctgc gcggtgctgt ggttatgtga cgaccacagc gtggtactga
    11161 aaccgttggg atatcccgcc tctttcagta attcgcgcgc tttcactgga tcgtaaggcc
    11221 acggtttata actttgcgcg taggcgatac ttggcggtac cacaccagta gctggagttg
    11281 catagcccgc aaaggcaact ttcaccagcg ccggacggtt aatggcgtaa ttcagcgcct
    11341 cacggacctt cgggttatcg aacggctttt gcgtcacgtt catactgata taacgctgca
    11401 taattgacgg actggccatc aactcgatat ttttgttttt ctccagcagt gtggcctgct
    11461 cgtagggaat ggggaaagca aactgcgctt caccggtttg cagcattgcc gcgcgggtgt
    11521 tgttatccgc caccggacgc caggttatgc tgtccagttt gggcaatcct ggctgccagt
    11581 aacccgcgaa ttttttcacc ttcacaaaat cggtctgatt ccaggtatcc agttcatacg
    11641 gtccggttcc caccggatga aaaccaatct ccttgccata tttttccagc gctgtcggtg
    11701 aaatcatcgc ggtcgccgga tgggcaagaa tattaataaa cgctgagaac ggctgtttga
    11761 gggtaatctt taccgttgtc ggatcgatcg cctccgtttt ggcaatattc ttatacaggt
    11821 tatagcgttt aagatgattc gccggatcgc tggcccggtc cagattcgct tttaccgcca
    11881 ctgcgttgaa atcggtgcca tcctggaatt taattccttc ccgcaatttc acggtgtaag
    11941 taatgccgtc atcggaaacg gtataactct ccgccagcac gtttttcagt ttcatctctt
    12001 tatccagacc gaacagcccc tggtaaaacg atttcgctac ggcctgagat aacgtgtcat
    12061 tcgcgtcata cggatcgagc gtggtgaaat tcgatcctac cgccaccacc acatctttgg
    12121 cagcgaatgc acaagatgcc atcaacgctg tcgcaatgcc cagcgccact aacccactac
    12181 ggtgtacagc tcttgccatt ttattctccg cctgaatgtt atctacgcat gaatgcgtat
    12241 tctgattgtg gttgtgcgac gtaatgcccc ggcccgacgc attgcaacga gacggctgcc
    12301 acctcttcgc cacgcagatg aatattgctg ggaagatcgt ccgacagcag tacacgctgc
    12361 ggtcgttgtc gggacggttc agcgaccgga actgccgcca gtaatttacg cgtataagga
    12421 tgctgcgggt tttcgaagac cgcgcaccgt gaaccaattt caacaatttg cccgagatac
    12481 atcaccgcca cacgatgact aatccgctct accacagcca tatcgtggga gataaacaga
    12541 tacgcaatgc cgaaatcacg ctggagatcg agcaacaagt tgataatctg cccgcgaata
    12601 gaaacatcca gcgccgaaac ggcttcgtcg gcaatgatca cttttggatt caatgccaac
    12661 gcgcgagcaa tgcagatgcg ctggcgctga ccgccggaaa actcatgcgg gtaacgccag
    12721 gcatgttcag gtaacaggcc cacgcgctcc agcaaccacg caacgcgtgc agccgcgtct
    12781 ttacctggca ataaaccgtg tacacgcagc ggttcgataa tcgaatcacc gatggtctga
    12841 cgtgggtcca gcgaagcgta agggtcctga aaaataaact gaatatcccg gcgcaatgcc
    12901 tgaagtttgc caggtgacaa ggtatcgatt cgctgaccgt taaagataat ttcgccgccc
    12961 tgcgattcga ccaggcgcag caacgcccgc ccggtagtgg atttaccgct gccagactcg
    13021 cccaccagcg atagcgtttc gccaggccag agatcaaaac tgactttctc aacggcatgc
    13081 acttcccgcg ttacgcgatt caacaaaccg ctgcgcaaag ggaaacgggt gacaagatta
    13141 cgcacccgta aaacaggttc gccatccacc accgttttct gctcgatggg gggttcctgt
    13201 ttcgctggat gttcaagcga tatcaatggg aaacgtcggg gataatctaa ccctttcatc
    13261 gcaccaagtt gcggaacagc agctaacagc gcacgggtgt aaggatgttg cggtgcatga
    13321 aaaatctgtt cgacggtacc cgtttccacc gcctcgccct gatacatcac cagtacccga
    13381 tcggcaatct ctgccaccac gcccatatcg tgagtgataa agataacgcc catcgacatc
    13441 tctttttgca atactttgat taattgcagg atctgcgcct gaatagtgac atccagcgcg
    13501 gtggttggct catcggcaat cagcaccgcc gggcggcatg acagcgccat cgcaatcatc
    13561 actcgctggc gcatcccgcc agagagttga tgcgaataac gtgaaagaat ggtttgtgcc
    13621 tccggaatgc gtacctgatc cagcatccgc ttcgcctcga ccatcgcttc ttcacgactg
    13681 gcgttctgat gcagacgaat tgattcggca atctgttcac ccacagtaaa tactgggttc
    13741 agcgatgtca tcggctcctg aaatatcatc gccatatccg caccgcgcac atggcgcatt
    13801 tgtgcagcgc tctgctcgct aagttcaatc acatcacgac tgcgccgccg caacagcatt
    13861 ttatcgcact gtactaaacc gcccgcctgt tccaacagac gcatcaatgc cagcgcagtc
    13921 actgacttac cggagccgga ttcgcctaca attgccagcg tctcaccgcg ttgcagacta
    13981 aaagagagat tgcggaccgc agctattttc tgctggtcct gcataaaggc aatattaaga
    14041 ttttcaaccg ccagcacatt accggcatca agttcatcac tgtgtggcaa cggtgtcccc
    14101 tttttcacgg tagataccgg tggttggcgt atcgcctgcg tagccccagg cgcgatacat
    14161 tccttcggtg ttaaacggta gcgcgacatt cccttcatgg tcgatagcga ttaagccacc
    14221 gctaccgcca agcgcaggga gtttttccat tactacccgc tcgcaggctt ccgcgagact
    14281 taatccgccg taatccatta acgcggcgat gtcatatgcc gccagcgcgc ggatgaagac
    14341 ttcgcctgtg ccggtacaag aaaccgccac actggcgtta ttggcgtagc atccggcacc
    14401 cactaaggga ctatcgccaa ctcgtccggg taatttattg gtcattccgc ctgtggacgt
    14461 ggctgccgcc agattgccgt ctaaatccaa cgccacggcc cccacggtgc ccattttctg
    14521 tttttcatcc agtggcgcac cgctatggtc gaggactgtt gccccttcct cgcgcgctgc
    14581 cagtagttgt tcataacgca aaggcgtgga gaaaatctcc ggtgagacgc gctccatgcc
    14641 atgagcaaac gcaaaatttt ctgccccttc gccaatcatc atcacatgcg ggctttgctc
    14701 catcaccagc cgggcggcaa gaaccggatt acgcagatga ctaacgcccg ccaccgcacc
    14761 ggctttcagg gtgttaccat ccatcacaca ggcgtccagt tcatgggttt catcacgcgt
    14821 aaagacagcg ccaattccgg cgttaaacag tggacactct tccagcagac gcaccgcttc
    14881 cgtcaccaca tccagcgcac tttcgccggc ttccagcatt ttctgcccgg tttcaacaat
    14941 ggcagacaac gcctcgatgt agcgtaattc ctgttgcaga ctcatctgcg cgcggctaat
    15001 tgcacctgcg ccaccatgaa ttgcaatgac tgctttgccc atagtattcg tccattaagg
    15061 gtggctcgcc acttttgcta taaatatcag aatttttatg aattactcat tcaatttttg
    15121 aatatagaaa gatggatatg gcatgtaaag gcaggagcca acgctacgcc cgaggtggca
    15181 tacagtctat ggcgtttttc tgacataata ggcaaattcg attttgcctc cgcaggagtg
    15241 ttttcatgga atttaccacc ggattgatgt cgctcgacac cgcgcttaat gagatgcttt
    15301 ctcgcgttac cccactgacc acccaggaaa cgctgccact ggttcagtgt tttggtcgta
    15361 ttctggcgag cgatgtcgtt tcgccactgg atgtcccggg gtttgataac tccgctatgg
    15421 acggctacgc ggtgcgttta gccgatattg cctccgggca accgctgccc gttgccggta
    15481 aatcctttgc cggtcagcca taccatggtg aatggcctgc gggtacctgc attcgtatta
    15541 tgaccggtgc gccggtgccg gaaggctgcg aagcggtggt gatgcaggag cagactgaac
    15601 aaacggacaa tggcgtgcgt tttactgctg aagtgcgtag cgggcaaaat attcgccgtc
    15661 gcggtgaaga tatctctgca ggtgcggttg ttttcccggc gggaactcgc ctgactaccg
    15721 cagagctgcc agtaattgct tcactgggga ttgccgaagt tccggtgatt cgtaaagtgc
    15781 gtgtagcgct tttttctacc ggtgatgaac tccagttgcc cggtcagccg ctgggcgacg
    15841 gccaaatcta cgacaccaac cgtctcgccg tacacctgat gttagaacag ctgggttgcg
    15901 aggtaatcaa cttagggatt atccgcgacg atccacatgc cctgcgcgcc gcatttattg
    15961 aagccgacag ccaggcggat gtagtgatca gttccggcgg tgtttcagtg ggtgaagcgg
    16021 attacaccaa aatgattctt gaagagctgg gggagatcgc cttctggaag ctggcgatta
    16081 aaccaggtaa accgttcgcg ttcggtaaac tcagcaatag ctggttctgc ggcctgccgg
    16141 gcaacccggt ttcagcgacg ctgaccttct atcaactggt acagcctttg ctggcaaaac
    16201 taagcggcaa caccgccagc ggcctgcccg cgcgccagcg cgtacgcaca gcgtcccgcc
    16261 tgaaaaaaac gccaggacgt cttgatttcc agcgcggcgt gctgcaacgc aacgccgacg
    16321 gtgaacttga agtgacgacc accggacatc agggttcaca tatatttagc tcctttagtc
    16381 tcggcaactg ctttatcgtg ctggaacgcg atcgcggcaa tgtggacgtg cgcgaatggg
    16441 tggaagtaga accgtttaac gcgttgttcg gaggcctgta atggcggaac tcagcgatca
    16501 ggagatgctg cgctacaacc ggcaaatcat tctgcgcggt tttgattttg acggtcagga
    16561 ggcgctaaaa gattctcgtg tgctggtagt cggcctcggt ggactcggct gcgcagcttc
    16621 gcagtatctg gcaagcgccg gtgtcggcaa cctgacgctg ctcgacttcg acacggtttc
    16681 gctctcgaat ctgcaacgcc agacactgca cagtgatgcc acggtcgggc aaccgaaggt
    16741 ggaatccgcc cgtgacgccc tgacgcggat caacccacat atcgcgatta cgccagtcaa
    16801 tgcactgcta gatgacgcag aacttgcagc attgattgct gaacacgatc tggtgctcga
    16861 ctgtacggat aacgttgcgg tacgtaatca actgaacgca ggctgttttg ccgcgaaggt
    16921 gccgctggtt tccggcgcgg caattcgtat ggaagggcaa atcacggtct tcacttatca
    16981 ggacggcgaa ccgtgctatc gctgccttag ccgtttgttt ggcgagaacg cgttaacctg
    17041 cgtagaagca ggcgtgatgg caccgttgat aggcgtaatt ggttcgttgc aggcaatgga
    17101 agcgatcaaa ctgctggcag gttatggcaa accggccagc gggaaaatcg tcatgtacga
    17161 tgcgatgacc tgtcagtttc gcgaaatgaa actgatgcgc aatccggggt gtgaggtgtg
    17221 cgggcagtaa ttgccacctc caagcctgat aagacgcgac aagcgtcgca tcaggcattt
    17281 gctccagacg ccagatgcgg cgtgaacgcc ttatctggcc tacggttcgg gcacagattt
    17341 ccaagcgcgg caggcatcgg tgcactccgc cacgtcatca gcaatttatg aggatgcaga
    17401 acgtccggtt aaatcgacgt tctgccaaac gctccctgcc agtcctgctc aaacttcgtc
    17461 acagcggctt ccaccgccgg atagctaatc atctgttgtg ccacatccag tggcagagta
    17521 attgattcac atccggccag taagcagtcc agcgcctgac gcggggtttt gaaactcgct
    17581 gccagcactt tcgcctgcgg cgcatgcatt ttcaataact ggtgtaagtc ggtcacagtc
    17641 tgaatgccgc taccgccctg agcatcaata cgattaacgt aaggcgcaac atattccgca
    17701 cctgccagcg ccgacagcag cccttgtgcc gcgccatata ccgcggttcc cagcgtcgga
    17761 atcccttccg cttttaacat cttaatcgct gccagcccct cggctgtcac cggaactttc
    17821 accacgatat ccgcaataat agaacgcagc ttaagcgcgt cattcaccat cccttcagca
    17881 gtggtagcca ttacctgggc aaacagacgc ccctgaccgc ccatcgcttc atgaagttgc
    17941 ggaagcacaa catccagcgg ttttttaccc gcggcgataa tgcttgggtt agtggtcaca
    18001 cccgccagcg gaaaaatacg tgacagcgcc ttcaccgcaa caacgtctga agtatccaga
    18061 tacagttcca taaccatcct caaaatgtac tttttgtctg aatgtagact atcacgggaa
    18121 aactctctga acagttgacg cacatcaatc taactttcat tcgaaagtat tttaatgttt
    18181 gaacgaaaca gagaggcaac tatgattttc aatattcagc gctactcgac ccatgatggc
    18241 cccggtatcc gcacggtcgt atttcttaaa ggctgttcgc tgggctgccg ctggtgtcag
    18301 aacccggaaa gccgcgcccg tacgcaggat ctgctgtatg acgcacgact gtgtctggaa
    18361 ggctgcgagc tgtgcgctaa ggccgcgccg gaagtgattg agcgcgcgct gaatggtttg
    18421 cttattcatc gggaaaagtt aaccccggag catctgacgg cgttaaccga ctgctgtccg
    18481 acacaggcgt taaccgtgtg tggtgaagtg aaaagcgttg aggagatcat ggcgaccgtt
    18541 ctgcgcgata aaccgtttta cgatcgcagc ggcggcggct taacgctttc gggtggtgaa
    18601 cccttcatgc agccggaaat ggcgacggcg ctgctgcaag cctgccacga ggcaggcatt
    18661 catactgcgg tagaaacctg tctgcatgtg ccgtggaaat atatcgcccc ttctctgccc
    18721 tatatcgatc tgtttcttgc cgatttaaaa cacgttgccg acgcgccgtt taaacagtgg
    18781 accgacggta acgccgccag ggtgctggat aacctgaaaa aactcgccgc agcgggcaaa
    18841 aaaatcatta tccgcgtgcc gctgattcag ggctttaatg ccgacgaaac ctctgtaaaa
    18901 gccattaccg attttgccgc cgacgagctg cacgttggcg aaattcattt tctgccctac
    18961 cacacgctgg gcatcaacaa atatcactta cttaatctgc cctatgacgc cccggaaaaa
    19021 ccgcttgatg cgccagagct gctcgatttt gcccagcagt atgcctgcca gaaagggtta
    19081 accgcgacct tacgaggata acaaccatga ccacactgaa actggacacg ctcagcgacc
    19141 gcattaaagc gcacaaaaat gcgctggtgc atattgtgaa gccgccagtc tgtaccgagc
    19201 gcgcgcagca ctataccgag atgtatcaac aacatctcga taagccgatc ccggtacgtc
    19261 gcgcgctggc actggcgcat cacctggcga atcgcaccat ctggatcaaa cacgatgagt
    19321 tgatcattgg caaccaggca agcgaagttc gcgccgcgcc gatcttcccg gaatatactg
    19381 tctcgtggat cgaaaaagag attgatgatc tggcagatcg tcccggtgct ggctttgcgg
    19441 tgagcgaaga gaacaaacgt gttctgcatg aagtgtgccc gtggtggcgc ggtcagacgg
    19501 tacaggatcg ctgctatggc atgtttaccg atgagcaaaa aggtctgctg gcgaccggca
    19561 tcattaaagc ggaaggcaat atgacctccg gcgatgcgca cctggcggtc aatttcccgc
    19621 tgctgctgga aaaagggctt gatggtctgc gtgagaaagt agcggaacgt cgctcgcgca
    19681 tcaatctgac ggtgctggaa gatttgcacg gcgaacagtt cctgaaagcg attgatatca
    19741 tgctggtggc agtcagtgaa cacattgaac gtttcgctgc cctggcgcgt gcaatggccg
    19801 cgaccgaaac ccgcgaaagc cgtcgcgatg aactgctggc gatggcagaa aactgcgatc
    19861 ttatcgccca ccagccgccg cagactttct ggcaggcgct gcaactgtgt tacttcatcc
    19921 agttgatttt gcagattgaa tctaacggtc actcggtgtc gtttggtcgt atggatcagt
    19981 atctctaccc gtactatcgc cgcgacgttg aactgaacca gacgctggat cgcgaacacg
    20041 ccatcgaatt gctgcatagc tgctggttga aattgctgga agtgaacaaa atccgctccg
    20101 gctcccactc aaaagcctct gcgggaagtc cgctgtatca gaacgtcacc attggcggac
    20161 aaaatctggt tgatggtcaa ccaatggacg cggtgaatcc actctcttac gcgatcctcg
    20221 aatcctgcgg tcgcctgcgt tccactcagc ctaacctcag cgtgcgttac catgcaggaa
    20281 tgagtaacga tttcctcgac gcctgcgtac atgtgatccg ctgcggcttc gggatgccgg
    20341 cattcaacaa cgacgaaatc gtgatcccgg aatttattaa actcggtatt gaaccgcagg
    20401 acgcttatga ctacgcagcg attggttgta tagaaaccgc cgtcggtggc aaatggggct
    20461 atcgctgtac cggcatgagc tttatcaact tcgcccgcgt gatgctggcg gcgctggaag
    20521 gcgggcgtga tgccaccagc ggcaaagtgt tcctgccaca agaaaaagcg ttgtcggcag
    20581 gtaacttcaa caacttcgat gaagtgatgg acgcgtggga tacgcaaatc cgttactaca
    20641 cgcgtaaatc aatcgaaatc gaatatgtcg tcgacaccat gctggaagag aacgtgcacg
    20701 atattctctg ctcggcgctg gtggatgact gtattgagcg agcgaaaagt atcaagcaag
    20761 gcggcgcgaa atatgactgg gtttctggcc tgcaggtcgg tattgccaac ctcggcaaca
    20821 gcctggcggc agtgaagaaa ctggtgtttg aacagggcgc gattggtcag caacagctgg
    20881 ctgccgcact ggcggatgac ttcgatggcc tgactcatga gcaactgcgt cagcggctga
    20941 ttaacggtgc gccgaagtac ggcaacgacg atgataccgt cgatacgctg ctggctcgcg
    21001 cttatcagac ctatatcgac gaactgaaac agtaccataa cccacgctac ggtcgtggtc
    21061 cggttggcgg caactattac gcgggtacgt cgtctatctc cgctaacgta ccgtttggcg
    21121 cgcaaactat ggcaacgccg gacgggcgta aagcccacac cccgctggca gaaggcgcta
    21181 gtccggcctc tggtactgac catctcggcc ctactgcggt cattggctca gtgggtaaac
    21241 tgcctacggc agcgattctc ggcggcgtgt tgctcaacca gaaactgaat ccggcaacgc
    21301 tggagaacga atctgacaag cagaaactga tgatcctgct gcgtaccttc tttgaagtgc
    21361 ataaaggctg gcatattcag tacaacatcg tttcccgcga aacgctgctg gaggcgaaaa
    21421 aacatccgga tcagtaccgc gatctggtag tgcgtgtcgc gggttattcc gccttcttca
    21481 ccgcgctctc tccagacgct caggacgata tcatcgcccg tactgaacat atgctgtaat
    21541 ctcttcggcc cggctgcggc gtcgccgggc taaatcattc acatcatcaa tttcatcctt
    21601 actttcattc gaaatataat ttgtgctctg cgtcacattg ttattacact gttacggttt
    21661 cattttcgag ccaggagcac acctatgagc gtaaaagtta tcgtcacaga tatggacggt
    21721 acttttctta acgacgccaa aacgtacaac caaccacgtt ttatggcgca atatcaggaa
    21781 ctgaaaaagc gcggcattaa gttcgttgtc gccagcggta atcagtatta ccagcttatt
    21841 tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg cggaaaacgg cgcactggtt
    21901 tacgaacatg gcaagcaact gttccacggc gaactgaccc gacatgaatc gcggattgtt
    21961 attggcgagt tgctaaaaga taagcaactc aattttgtcg cctgcggtct gcaaagtgca
    22021 tatgtcagcg aaaatgcccc cgaagcattt gtcgcactga tggcaaaaca ctaccatcgc
    22081 ctgaaacctg taaaagatta tcaggagatt gacgacgtac tgttcaagtt ttcgctcaac
    22141 ctgccggatg aacaaatccc gttagtgatc gacaaactac acatagcgct ggatggcatt
    22201 atgaaacccg tcaccagtgg ttttggcttt atcgacctga ttattcccgg tctacataaa
    22261 gcaaacggta tttcgcggtt actgaaacgc tgggatctgt caccgcaaaa tgtggtagcg
    22321 attggcgaca gcggtaacga tgcggagatg ctgaaaatgg cgcgttattc ctttgcgatg
    22381 ggcaatgctg cggaaaacat taaacaaatc gcccgttacg ctaccgatga taataatcat
    22441 gaaggcgcgc tgaatgtgat tcaggctgtg ctggataaca catccccttt taacagctga
    22501 cctctctacc ggagccatgc gctccggttt tcccttcttt ttacatcctg tacatcacat
    22561 caaatcatta aacttgcatt aacttattta taacttgtat tgtcacatgc aaattagcat
    22621 agtttcgaat gaaagttaat gcaaggtggt tatggcttct acttttacca gcgacacatt
    22681 gcctgccgat cacaaagcag ctatccgtca gatgaagcac gcgctgcggg cgcagcttgg
    22741 cgatgtccag cagatcttta atcagctaag cgatgacatt gccacgcgag tggctgaaat
    22801 caacgcactc aaagcacagg gcgatgccgt ctggccggtg ctgtcttatg ccgatatcaa
    22861 agcaggtcat gttactgcag agcagcgcga acagattaaa cgtcgcggtt gtgcggtgat
    22921 aaaaggccat ttcccccgcg aacaagcgct aggctgggat cagtcgatgc tggactatct
    22981 gaaccgcaac cgctttgacg aggtctacaa aggccccggc gataatttct tcgggacgct
    23041 cagcgcttca cgtcccgaga tttaccccat ctactggtcg caggcgcaaa tgcaggcccg
    23101 ccagagtgaa gaaatggcga atgcgcagtc gtttctcaat cgtctgtgga catttgaaag
    23161 tgatggaaag caatggttta acccggatgt gagcgtcatc taccctgacc gtatccgccg
    23221 ccttccgccc ggaacgacct ccaaaggtct tggagcgcat accgactccg gggcgctgga
    23281 acgctggctg cttccagcgt atcagcacgt tttcgccaac gtctttaatg gcaatctggc
    23341 gaaatacgat ccctggcatg cggcacatcg tacggaagtt gaagagtaca cggtggacaa
    23401 caccaccaaa tgttccgtgt ttcggacatt ccagggctgg actgcgctct cggatatgct
    23461 gcctggtcag gggttgctgc acgtcgtgcc cattcctgaa gccatggcgt acgtactgtt
    23521 acgtccgctg cttgatgatg tgccggagga tgaactgtgc ggcgtagcgc ccggaagagt
    23581 gttgccggta tcagagcaat ggcatccact gttaattgag gcgttaacca gcattccaaa
    23641 actcgaggcc ggagactccg tctggtggca ctgcgacgtc atccattccg ttgcccccgt
    23701 tgaaaatcaa cagggttggg gcaacgtgat gtacattcct gcggcaccga tgtgcgagaa
    23761 aaatcttgcc tacgcgcaca aggtgaaggc cgcactggaa aaaggcgcat cgccgggcga
    23821 cttcccgcgc gaggactatg aaacaaactg ggaaggacgc tttacgcttg ccgacctcaa
    23881 cattcacggt aagcgagcgt tgggcatgga tgtttgagag ttaatggaag tgaattgatg
    23941 tgggattaag aaaatcgatt cgacgttact cagattgctg acaagtgcgc tttcttaatg
    24001 cctgatgcgg cgttaacgcc ttatccggcc tacaaagtcc tgcagattca atatattaca
    24061 ggtactgcgt aggcctgata agcgtagcgc atcaggctat tttgcatttg tcatcagccg
    24121 tcgtcacccc gacagtatcg gggtgacgga taatcactta ctcgatccct ttactacgca
    24181 ggtaatcttc gtaattaccg ctaaagtcga tcacgcgttc cggggtgatt tccagaatgc
    24241 gggtcgccag ggagcttacg aactcacggt cgtgtgaaac aaagatcagc gtgccctgat
    24301 acagttccag cgccatgttc agtgactcga tggattccat atccaggtgg ttggtcggtt
    24361 cgtccatgat cagaatgttc ggcttctgca tcattaactt accaaacagc atccgccctt
    24421 tttccccacc ggaaagcact ttagctggct ttttgatgtc gtcctggctg aacagcaaac
    24481 gaccgagaat gctgcgtacc gcctgctcgt catcgccttc ctgcttccac tgactcatcc
    24541 attcgaacac ggtcagatca ttttcaaact catattcgtg gtcctgggcg tagtaaccaa
    24601 tgcgcgcgtt ttcagaccat tttacggtgc cgctgtccgg ttgcagatcg cccaccagcg
    24661 ttttcagcag cgttgattta ccgacgccgt tggtacccag taccgcaagt ttttcaccga
    24721 cctccagcag caggttgaga tttttaaaca gtggaccgtt atcaaaccct ttggtcagac
    24781 cttccacttc cagcgcgtta cggaacagtt tcttatcctg ttcaaaacgg ataaacgggt
    24841 tctgacggct ggaggctttc acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg
    24901 aagttgcctg gcgagatttc gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact
    24961 cagcaatctg cgctttcttc ttggcgttat cggccagcag acgttcacgc gcttgggtcg
    25021 ccgccgtcat gtactcatcg tagttacccg gataaacgcg cagctcgccg taatccagat
    25081 ccgccatgtg ggtacagacc atgttaagga agtgacggtc gtgcgagatg atgatcatgg
    25141 tgctgtcacg ctcgttcagc acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt
    25201 tggtcggttc gtcgagcagg agaatatccg gctcagcaaa cagcgcctgc gccagaagca
    25261 cacgcagctt ccagccagga gcaacttcac tcatcgggcc gtagtgctgt tccactggaa
    25321 ttcccacgcc aagcaacagt tcaccggcgc gagcttccgc agagtaaccg tccatttcgc
    25381 cgtatttcac ttccagatcg gccactttat agccgtcttc ttcactcatt tccggcaaag
    25441 catagatgcg gtcgcgctcc tgcttcactt cccacaactc tttatgcccc atgatcaccg
    25501 tatccagcac agtgaactct tcaaaggcaa actgatcctg acgcagctta ccaatgcgct
    25561 cgttgggatc gagggaaacg ttacccagcg tcggctcaag gtcgccgccg agaatcttca
    25621 taaaggtgga tttaccacta ccgttcgcgc caatcaggcc gtaacggttg ccgccgccga
    25681 atttgacgga aatgttttca aacaacggct tactgccgaa ctgcatggtg acgttactgg
    25741 aaactaacac aggcgtatcc tgaaaagaga tatgacaaac cgcgcattat gccataagtc
    25801 tgaaagaaga tcctgctcct cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga
    25861 tttcgtacac atctgatttc actgtgagct ggaatgaact tataatgcgc ttccaatact
    25921 ctaatattct caacccaatg gcctgccagg cacaaaatct cgcttaacat gaatatgaaa
    25981 ttgaaaacat tattcgcagc ggccttcgct gttgtcggct tttgcagtac cgcctctgcg
    26041 gtaacttatc ctctgccaac cgacgggagt cgcctggttg gtcagaatca ggtgatcacc
    26101 attcctgaag gtaacactca gccgctggag tattttgccg cggagtacca gatggggctt
    26161 tccaatatga tggaagcgaa cccgggtgtg gataccttcc tgccgaaagg cggtactgta
    26221 ctgaacattc cgcagcagct gatcctgccg gataccgttc atgaaggcat cgtcattaac
    26281 agtgcagaga tgcgtctgta ttactatccg aaagggacca acaccgttat cgtgctgccg
    26341 atcggcattg gtcagttagg caaagatacg cctatcaact ggaccaccaa agttgagcat
    26401 aagaaagcag gcccgacctg gacgccgacc gccaaaatgc acgcagagta ccgcgctgcg
    26461 ggcgaaccgc taccggctgt cgttccggca ggtccggata acccgatggg tctgtatgca
    26521 ctctacatcg gtcgcctgta tgctatccat ggcaccaacg ccaacttcgg tatcggcctg
    26581 cgtgtaagtc atggttgtgt gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa
    26641 gtaccggtcg gtacccgcgt acagtttatt gatgagccgg taaaagcgac caccgagcca
    26701 gacggcagcc gttatattga agtccataac ccgctgtcta ccaccgaagc ccagtttgaa
    26761 ggtcaggaaa tcgtgccaat taccctgacg aagagcgtgc agacagtgac cggtcagcca
    26821 gatgttgacc aggttgttct tgatgaagcg atcaaaaatc gttccgggat gccggttcgc
    26881 ctgaattaat cgtcaaaacg taaaacaaag gcggactcat aatccgcctt ttttatttgc
    26941 cagaccttac caggccggga tcataactaa caaaacgtat cccaccagcg ccgcccacaa
    27001 cagcatcggt attgaataga gatggaaacg ccaccagatg cggcgatcgt tcgccatacg
    27061 tagcgcaatc aaatttgcca gcgatcctgg taataaacca aagcccccca cgtttaccgc
    27121 ccatgccagt aataaagacg gaggtacata attgagcagc aaaatggtac tcggcacgtt
    27181 actgatcacc tgcgataaac cgactgccgt taaccataac ccgggttcag ataaatgact
    27241 cacgttaccc aacacgcctt gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa
    27301 catcgccata aacaccagca gcaacgtcca gtccacactc aacaccacgc gacgcgccag
    27361 cagcacaaag cccgccgcca caatcaccag tccccacagc tcttgtttga actccagcgc
    27421 cgtcagaaag acgatataca gccccagaca attccacacc agccgcggtt tccactccgg
    27481 tgtttgcacc cccgtatggt attgcagcgc ctttccaggg aaacaacacc agcacaacag
    27541 caggagtgtc agcatcattg cgccagccat cggtgccatt tgggcaataa atccggcaaa
    27601 cgaaagacca gaacgtcccc agataagaat attttgcggg ttgccaattg gcgtcagtag
    27661 cgaaccagcg ttgactgcca gcgcctcaaa aataatcagc cgattaaccg ggatctcaca
    27721 cagtcttttt agcgtgatag tcagcggaac aacaataaac agcgcgacat cgttggtcag
    27781 aaaggtagaa agcagcgccg ccgccagcac cataaacatc gccagccgac gctccgtagc
    27841 aaagcggcgc accattttgc gccccagcac atcaaaataa ccgcttaact ccacaccttt
    27901 ggtcagcagc atcaggccgc ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca
    27961 ggatttcggt gcaaagggca cgaaaaagct taatccgata ccaacaagaa ttaataactg
    28021 aaaaaaacga tcgccttgca gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc
    28081 tgggtaaaca aacgaaaagc gtcgagcgtc tcttcactaa catggtgctc catgccttcc
    28141 gcgtcgcgac gggcgatttc cggactgacg cccaacacca acaagaaatt ttcgactatc
    28201 tgatgtcgct cgcggctttc ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca
    28261 cgccagggga tcatttcaat cagccccatg gttgccagcc gcttaagcat tttagccacc
    28321 gtcggttgcg aaactcccag acgagcagcc atgtccacct gacgagcttc ccccacttcc
    28381 ctgatcaagt cagaaatcag ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct
    28441 ctgacctggc ggaacccttc aacgtgctct tccacgttca ctaactgcgt cacttttttt
    28501 gctattggcg tacctgcgcg acgactcatt gtgcttcctc attatggtga cgcatacata
    28561 gcgttctcat ttcgtgcgca cattgtaaac cagagttgcg aaggtacaaa aaattaacgt
    28621 tttagcaata gctatataat atagcctgtg ctatatctgt atgtaatgca atcatccctc
    28681 aaggatcgac gggattagca agtcaggagg tcttatgaat gagttcaaga ggtgtatgcg
    28741 cgtgtttagt cattctccct ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat
    28801 ggtcaacaac aaaccgcagc aagataaacc ttcagataaa tagcggcgtc gcggtacgcc
    28861 gcttcactcc tgcttttatg caggcataac gcgttttggt ctgaaaaaaa ccactttttg
    28921 tcggatttgc aatccccttc gcaaaagatt tgttcgtcag tagttgacct gaacggcggc
    28981 tcgctctatc ttcttgcagc cctgcgtata ttgcggctcg cggatgcgga cccctttccc
    29041 ctcttcacgc actcttgcag gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt
    29101 tacgatagtg gcatatgaat ttaaccctca aagaatcgct tgttacccgt agccgggtat
    29161 ttagcccgtg gactgcgttc tactttttac agtcgctatt aattaacctc ggcttaggtt
    29221 accccttcag tttgctctac accgctgcgt ttacggctat tttgcttttg ctatggcgaa
    29281 cattgcctcg cgtacaaaaa gttctggtcg gtgtcagttc gctggtggcg gcttgttatt
    29341 tcccttttgc tcaggcctac ggcgcgccta atttcaatac attgctggca ttgcactcca
    29401 ccaatatgga agagtcgacc gaaatcctga cgatttttcc gtggtacagc tacctggtcg
    29461 gcttatttat ttttgcgctc ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag
    29521 cgcgctggaa taccttcgac agcctgtgtc tggtattcag tgtggcgaca ttttttgttg
    29581 ctcccgtgca aaacctggcc tggggtggcg tatttaaact gaaagatact ggctatccgg
    29641 tatttcgttt tgctaaggat gtcatcgtca ataataacga ggtgattgaa gagcaagaac
    29701 ggatggcaaa actttccgga atgaaagata cctggacggt cactgccgtt aagccgaagt
    29761 atcagaccta tgtggtagtg atcggtgaaa gcgcgcgtcg cgatgccctc ggtgcctttg
    29821 gcggtcactg ggacaatacc ccgtttgcca gcagcgttaa cggtttgata tttgctgact
    29881 acattgctgc cagtggctcc acgcagaaat cgcttggctt aacgctcaat cgcgtagtcg
    29941 atggcaaacc acagtttcag gataactttg tcaccctggc aaatcgcgcg ggcttccaga
    30001 cctggtggtt ttccaaccag ggccaaatcg gcgaatacga tactgctatc gccagcatcg
    30061 ccaaacgtgc agatgaagtg tacttcctga aagaaggtaa ttttgaagca gataaaaaca
    30121 cgaaagacga agcgttactg gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac
    30181 cgcagctgat tgttctgcat ctgatgggct cgcatccgca ggcctgcgac aggacacaag
    30241 gcaaatacga aacctttgtg caatcgaaag aaacgtcgtg ctatctctat accatgacgc
    30301 aaacggacga tttactgcgc aagctgtacg atcagttacg caacagcggc agcagcttct
    30361 ctctggttta cttttctgac cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat
    30421 accttgccca tgatgataaa tatcagcaaa atttccaggt gccttttatg gtcatttcca
    30481 gcggcgataa agcgcatcgg gtgattaaag cccgccgctc agccaatgac ttcttaggct
    30541 ttttctcgca gtggacggga attaaagcga aggaaataaa tatcaaatac ccgtttatat
    30601 ctgagaagaa agccgggccg atatacatca ccaacttcca gttacagaag gtggattaca
    30661 accatctcgg aaccgatatt ttcgacccga aaccttaaaa acaaaaatcc gccccgagag
    30721 gcggattttt atatcaccaa agtgattaga agcggtaacc aacaccggca atccaggtgc
    30781 ctacgtcaac gctacgaata cggctctgct cgtaagagaa gtccagagca acgttttcca
    30841 tcgggttgaa ctgcagacca gcaccgtagg agaaaccgta gtcgctggtg tcgtgtttgt
    30901 aggtcgggta ttcagtggtc tggaatttac cataacccac acccactaca ccgtagatgc
    30961 ttgcccagtc gttaatgcgg taagccggac cagcagtgat gccgtagtac tggtttttgt
    31021 tgtagtcacc agagcttgca gtacggcttt tctcggtgta agtgaaagaa ccgatcacac
    31081 ccagcgggct gttgtcttct tcatagcggt atttcaggtt gaaaccgccc attttgttca
    31141 tttggccctg agcgtcgctc tgtgcgtaac cgccagttac ggtagaagtc gcagctacgg
    31201 aagtacctgc ggtgaaagcc agaactgcgg ccagtgctga aagacatgca atttttttca
    31261 taaccacctc aaatgtgatt caaataagtc ctaagtttta aatatatcaa aaattaatgg
    31321 gaaactcttc gcgatttgtg atgtctaacg ggccatttca tgtaacagaa cgtttccata
    31381 caccgctatc catctaaatt taaatcactt tttcagagaa ctgcgtaagt attacgcatg
    31441 ttttccctgt cattcatcca gattattcct aatcaccaga ctaatgattc catcaatcct
    31501 ggcgcatttt agtcaaaacg ggggaaaatt ttttcaacaa atgctcaacc agcattgggt
    31561 atatccagta cactccacgc tttacttaag tctagatatt tgtgggagaa aggatgcctg
    31621 gttcattacg taaaatgccg gtctggttac caatagtcat attgctcgtt gccatggcgt
    31681 ctattcaggg tggagcctcg ttagctaagt cactttttcc tctggtgagc gcaccgggtg
    31741 tcactgcgct gcgtctggca ttaggaacgc tgatcctcat cgcgttcttt aagccatggc
    31801 gactgcgctt tgccaaagag caacggttac cgctgttgtt ttacggcgtt tcgctgggtg
    31861 ggatgaatta tcttttttat ctttctattc agacagtacc gctgggtatt gcggtggcgc
    31921 tggagttcac cggaccactg gcggtggcgc tgttctcttc tcgtcgcccg gtagatttcg
    31981 tctgggttgt gctggcggtt cttggtctgt ggttcctgct accgctgggg caagacgttt
    32041 cccatgtcga tttaaccggc tgtgcgctgg cactgggggc cggggcttgt tgggctattt
    32101 acattttaag tgggcaacgc gcaggagcgg aacatggccc tgcgacggta gcaattggtt
    32161 cgttgattgc agcgttaatt ttcgtgccaa ttggagcgct tcaggctggt gaagcactct
    32221 ggcactggtc ggttattcca ttgggtctgg ctgtcgttat tctctcgacc gctctgcctt
    32281 attcgctgga aatgattgcc ctcacccgtt tgccaacacg gacatttggt acgctgatga
    32341 gcatggaacc ggcgctggct gccgtttccg ggatgatttt cctcggagaa acactgacac
    32401 ccatacagct gctggcgctc ggcgctatca tcgccgcttc aatggggtct acgctgacag
    32461 tacgcaaaga gagcaaaata aaagaattag acattaatta aatttacatt tctgtatggt
    32521 tatgcataac catgcagaat ttctcgctac ttttcctcta caccgtattt atatatcgaa
    32581 ttatgcaaaa gcatatttat tccgaaaatt cctggcgagc agataaataa gaattgttct
    32641 tatcaatata tctaactcat tgaatcttta ttagttttgt ttttcatgct tgttaccact
    32701 attagtgtga taggaacagc cagaatagcg gaacacatag ccggtgctat acttagtctc
    32761 gttaattact gggacataac atcaagagga tatgaaatta tgagtaccgc taaattagtt
    32821 aaatcaaaag cgaccaatct gctttatacc cgcaacgatg tctccgacag cgagaaaaaa
    32881 gcaacagtag agttgctgaa tcgccaggtt atccagttta ttgatctttc tttgattacc
    32941 aaacaagcgc actggaacat gcgcggcgct aacttcattg ccgtacatga aatgctggat
    33001 ggcttccgca ccgcactgat cgatcatctg gataccatgg cagaacgtgc cgtgcagctg
    33061 ggcggtgtag ctctggggac cactcaagtt atcaacagca aaaccccgct gaaaagttac
    33121 ccgctggaca tccacaacgt tcaggatcac ctgaaagaac tggctgaccg ttacgcaatc
    33181 gtcgctaatg acgtacgcaa agcgattggc gaagcgaaag atgacgacac cgcagatatc
    33241 ctgaccgccg cgtctcgcga cctggataaa ttcctgtggt ttatcgagtc taacatcgaa
    33301 taaatccatc gctgatggtg cagaacttta gtacccgata aaagcggctt cctgacagga
    33361 ggccgttttg ttttgcagcc cacctcaacg cacttattta gtgcatccat ctgctatccc
    33421 cagctgatta agtaaatttt ttgtatccac atcatcacac aatcgttaca taaagattgt
    33481 tttttcatca ggttttacgc taaataatca ctgtgttgag tgcacaattt tagcgcacca
    33541 gattggtgcc ccagaatggt gcatcttcag ggtattgccc tataaatcgt gcatcacgtt
    33601 tttgccgcat ctcgaaaaat caatgagttg caaaattggc acgatttttt catatatgtg
    33661 aatgtcacgc aggggat
//
LOCUS       DAJTRH010000036        33447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTRH010000036 DAJTRH010000000
VERSION     DAJTRH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO339_001868"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO339_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5926520.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO339_001869"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO339_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5926521.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO339_001870"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO339_001870"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5926522.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO339_001871"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO339_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5926523.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO339_001872"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO339_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5926524.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO339_001873"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO339_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5926525.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO339_001874"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO339_001874"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5926526.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO339_001875"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO339_001875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5926527.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO339_001876"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO339_001876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5926528.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO339_001877"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO339_001877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5926529.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO339_001878"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO339_001878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5926530.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO339_001879"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO339_001879"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5926531.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO339_001880"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO339_001880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5926532.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO339_001881"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO339_001881"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5926533.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO339_001882"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO339_001882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5926534.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO339_001883"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO339_001883"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5926535.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO339_001884"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO339_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5926536.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO339_001885"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO339_001885"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5926537.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19508..20329
                     /locus_tag="OO339_001886"
     CDS             19508..20329
                     /locus_tag="OO339_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5926538.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20316..22424
                     /locus_tag="OO339_001887"
     CDS             20316..22424
                     /locus_tag="OO339_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5926539.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22421..24088
                     /locus_tag="OO339_001888"
     CDS             22421..24088
                     /locus_tag="OO339_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5926540.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24091..25617
                     /locus_tag="OO339_001889"
     CDS             24091..25617
                     /locus_tag="OO339_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5926541.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25618..27234
                     /locus_tag="OO339_001890"
     CDS             25618..27234
                     /locus_tag="OO339_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5926542.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27290..27442)
                     /locus_tag="OO339_001891"
                     /pseudo
     CDS             complement(27290..27442)
                     /locus_tag="OO339_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27465..27842)
                     /locus_tag="OO339_001892"
     CDS             complement(27465..27842)
                     /locus_tag="OO339_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926543.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28252..28623)
                     /locus_tag="OO339_001893"
     CDS             complement(28252..28623)
                     /locus_tag="OO339_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926544.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28684..29181)
                     /locus_tag="OO339_001894"
     CDS             complement(28684..29181)
                     /locus_tag="OO339_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926545.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29257..30045)
                     /gene="aadA1"
                     /locus_tag="OO339_001895"
     CDS             complement(29257..30045)
                     /gene="aadA1"
                     /locus_tag="OO339_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5926546.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(30103..30627)
                     /gene="sat2"
                     /locus_tag="OO339_001896"
     CDS             complement(30103..30627)
                     /gene="sat2"
                     /locus_tag="OO339_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5926547.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(30722..31195)
                     /locus_tag="OO339_001897"
     CDS             complement(30722..31195)
                     /locus_tag="OO339_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5926548.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            31527..32063
                     /locus_tag="OO339_001898"
     CDS             31527..32063
                     /locus_tag="OO339_001898"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5926549.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            32178..32504
                     /locus_tag="OO339_001899"
     CDS             32178..32504
                     /locus_tag="OO339_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5926550.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(32692..32931)
                     /locus_tag="OO339_001900"
     CDS             complement(32692..32931)
                     /locus_tag="OO339_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5926551.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         8674 a         7804 c         8447 g         8522 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg atcgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc
    19381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
    19441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
    19501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
    19561 agaggggcgt ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt
    19621 tccttcttca ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt
    19681 gctatctgac ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga
    19741 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
    19801 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
    19861 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
    19921 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
    19981 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
    20041 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
    20101 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
    20161 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
    20221 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
    20281 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
    20341 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
    20401 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
    20461 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
    20521 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
    20581 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
    20641 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
    20701 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
    20761 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
    20821 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
    20881 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
    20941 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
    21001 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
    21061 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
    21121 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
    21181 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
    21241 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
    21301 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
    21361 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
    21421 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
    21481 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
    21541 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
    21601 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
    21661 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
    21721 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
    21781 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
    21841 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
    21901 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
    21961 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
    22021 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
    22081 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
    22141 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
    22201 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
    22261 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
    22321 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
    22381 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
    22441 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
    22501 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
    22561 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
    22621 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
    22681 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa
    22741 tggttatgag cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc
    22801 tacggcacaa agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca
    22861 tcgtattcta gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt
    22921 ggtgtatttg aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt
    22981 tttcagagcg ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg
    23041 tcatggtata gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg
    23101 gttgttggtt attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga
    23161 gatgctgaac ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg
    23221 tacccctaaa gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg
    23281 gtttggagct atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga
    23341 gtggatcgct tttacggata atctttggca attacagctt ttacaacgca aagatgcgct
    23401 gttatcggat gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt
    23461 agtaaaactt tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac
    23521 cgctggttta ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga
    23581 ggcattacgc tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga
    23641 gattgataaa cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc
    23701 agaagaaaaa gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct
    23761 gaaagaggat tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa
    23821 tccaacgatg acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga
    23881 aacggtagtg tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt
    23941 agtcaagccc agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca
    24001 acgccaacct gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat
    24061 ggcgagctta tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat
    24121 gagctgattt atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag
    24181 cagctgttgg atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg
    24241 catttaggtg tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt
    24301 atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac
    24361 gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc
    24421 gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt
    24481 cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca
    24541 tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat
    24601 caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct
    24661 caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag
    24721 ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg
    24781 ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt
    24841 agtgatgagg cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg
    24901 ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg
    24961 tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt
    25021 actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat
    25081 ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg
    25141 gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat
    25201 tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt
    25261 gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta
    25321 gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac
    25381 ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt
    25441 tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt
    25501 aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa
    25561 gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg
    25621 gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt
    25681 aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa
    25741 tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca
    25801 aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc
    25861 aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg
    25921 cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca
    25981 cagttagagc tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg
    26041 agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta
    26101 gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta
    26161 gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt
    26221 tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc
    26281 tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag
    26341 gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc
    26401 acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag
    26461 cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag
    26521 gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac atggataagt
    26581 tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt
    26641 ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca
    26701 cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac
    26761 aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact
    26821 aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc
    26881 cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt
    26941 aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct
    27001 accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg
    27061 cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga
    27121 caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga
    27181 gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat
    27241 gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc
    27301 catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga
    27361 tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg
    27421 attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg
    27481 tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg
    27541 gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt
    27601 gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga
    27661 aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa
    27721 gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca
    27781 tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac
    27841 acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt
    27901 cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt
    27961 gatatttgaa acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc
    28021 ttgtaaatat cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta
    28081 tagaaatcgt cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca
    28141 tcgccatacc ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga
    28201 gcttgtacgc aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc
    28261 catccattcg gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta
    28321 tgttgcctgc accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc
    28381 ccacttgccc acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc
    28441 ctcctttaac tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc
    28501 gacatagtct ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt
    28561 gtcatacatg acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga
    28621 cactagtggt tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc
    28681 aggctagttg cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg
    28741 gccatttatg acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg
    28801 cccccctttt ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc
    28861 attgttggca atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag
    28921 cctacgattt ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc
    28981 agagttgtcg taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct
    29041 tggagaaatg tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa
    29101 aacaattggc aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac
    29161 caccttcaac agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc
    29221 cgcgaagcgg cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat
    29281 ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc
    29341 ttcttgtcca agataagcct gcctagcttc aagtatgacg ggctgatact gggccggcag
    29401 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
    29461 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
    29521 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
    29581 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
    29641 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
    29701 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
    29761 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
    29821 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
    29881 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
    29941 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
    30001 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactcctg
    30061 aattaagccg cgccgcgaag cggtgtcggc ttgaatgaat tgttaggcgt catcctgtgc
    30121 tcccgagaac cagtaccagt acatcgctgt ttcgttcgag acttgaggtc tagttttata
    30181 cgtgaacagg tcaatgccgc cgagagtaaa gccacatttt gcgtacaaat tgcaggcagg
    30241 tacattgttc gtttgtgtct ctaatcgtat gccaaggagc tgtctgctta gtgcccactt
    30301 tttcgcaaat tcgatgagac tgtgcgcgac tcctttgcct cggtgcgtgt gcgacacaac
    30361 aatgtgttcg atagaggcta gatcgttcca tgttgagttg agttcaatct tcccgacaag
    30421 ctcttggtcg atgaatgcgc catagcaagc agagtcttca tcagagtcat catccgagat
    30481 gtaatccttc cggtaggggc tcacacttct ggtagatagt tcaaagcctt ggtcggatag
    30541 gtgcacatcg aacacttcac gaacaatgaa atggttctca gcatccaatg tttccgccac
    30601 ctgctcaggg atcaccgaaa tcttcatatg acgcctaacg cctggcacag cggatcgcaa
    30661 acctggcgcg gcttttggta caaaaggcgt gacaggtttg cgaatccgtt gctgccactt
    30721 gttaaccctt ttgccagatt tggtaactat aatttatgtt agaggcgaag tcttgggtaa
    30781 aaactggcct aaaattgctg gggatttcag gaaagtaaac atcaccttcc ggctcgatgt
    30841 ctattgtaga tatatgtagt gtatctactt gatcgatcag gcttttgtat atctccccac
    30901 cacctgaaac aatgacatga tccgttattt tctttaggtt ggttaaagca tctttaattg
    30961 atggaaagat caatacgttc tcattgtcag atgtaaaact tgaacgtgtt acgaccgcat
    31021 actttcggtt gggtaatgct cccattgatt caaaagtctt gcgtccaacc aacagccatt
    31081 ggttataggt aatagcttta aacaggagct gttcaccttt ggcactccat ggaatatcag
    31141 ggccattccc gataactcca ttcttcgata tagctaccat tagtgatagt ttcacaattc
    31201 ttcctcagag gttaacattt tattagtaag catgctcgtt ttgtcacccg ctgatgctta
    31261 ccgttaatta atataatcac ttgtattaaa tagactttct ttaaaaatac aagacactct
    31321 gttattacaa atcgtgcatg ccgtctatcc tgcttgcacg atgcacatta tctcacccaa
    31381 agaactcata acattttaat ttatttagca ttaaattaaa actctaccgg taaacaagca
    31441 tctctaggcg taaaaaacag gcactttttt aaatcccagc tagaataggc tgtataggca
    31501 gacagttgca agacaaggag cgttttatgt ctaacagtcc atttttaaat tctatacgca
    31561 cggatatgcg acaaaaaggt tatgcgctga aaactgaaaa aacttacctg cactggatta
    31621 agcgttttat tctgtttcac aaaaaacgtc atcctcagac catgggcagt gaagaggtca
    31681 ggctgttttt atccagctta gcaaacagca gacatgtagc cataaacacg cagaaaatcg
    31741 ctttaaatgc cctagctttt ttgtacaaca ggtttttaca acagccgttg ggcgatattg
    31801 attatatccc tgcaagcaag cctagacggc taccctctgt tatctctgca aatgaagtgc
    31861 aacgcatttt gcaggttatg gatactcgca accaagttat ttttacgctg ctgtatggtg
    31921 caggtttgcg cattaatgaa tgcttgcgtt tgcgggttaa agattttgat tttgataatg
    31981 gctgcatcac tgtgcatgac ggtaagggtg ggaaaagcag aaacagccta ctgcccacgc
    32041 gcctaatccc agcaataaaa taactcattg agcaagcgcg gcttattcag caagacgaca
    32101 acttacaagg cgtagggcca tcgctgcctt ttgctttaga tcacaaatac ccttctgctt
    32161 atcgacaagc ggcgtggatg tttgtctttc cctccagcac gctctgcaac cacccgtata
    32221 acggcaaatt atgccgccat catctgcatg actccgttgc gcgaaaggca ttgaaggcag
    32281 ccgtacaaaa agcaggcatc gttagcaagc gtgtcacttg tcatacattt cgtcactcgt
    32341 ttgctacgca tctattacaa gcggggcgtg atattcgcac tgtgcaagaa ctcttagggc
    32401 ataacgatgt taagaccacg caaatctata cgcatgtgtt gggtcagcat tttgccggca
    32461 ccaccagtcc tgcggatgga ctgatgctac ttatcaatca gtaaaagtag ataaatatct
    32521 ttatgtagcc ctgagtagcc cctgatactt tttatggtga aaaagataaa caatcataga
    32581 ggggataaat atgagccagt tctaccattg ttcagataag tttaagcaag atactgtcaa
    32641 tcacagtgac cattcatggt cattacactt cctccagttg ccagacaatg cctaacataa
    32701 aaacgcaaaa gcccaaaagt cagagcttag gtgttctagg cgttttctaa gcgctgaaga
    32761 aatacgtctt accgtagatt gcgtgcgttt ctgaagcaca ttttccttat acatggcttt
    32821 atcccaggca gcttcatcaa gctcttggag taacaagcca gtaataatgc gacactctcg
    32881 ggtttgtagc gagccgctgg tgagatcgga gttatagcgg aaaggggtca tttgtaatta
    32941 tttgtgggtg gaggatagtt aacttattat tgtagttgga agaggctgct ttccaaacat
    33001 tagcacctcg taatgcttgt tactgtatat taatttatgc tcttagcgca tgttaatggg
    33061 ctattacaat gcataatgtc cacatcaaga taactgtggc ttaacacctg tacaagcgaa
    33121 aaaaatcaat cacctgtccg gaaatacggg atcactgtaa agtgttggtg aggcagtcta
    33181 ggagggggat ttaatttggg atactgatga aaggattatg atcagttgga tataggatcg
    33241 tactaagctg agcggcaggt tagctagatt agttaaccag ataagtgaaa tctagttcca
    33301 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
    33361 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
    33421 aactattttg tccgcccaca gctgccg
//
LOCUS       DAJTRH010000037        33156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTRH010000037 DAJTRH010000000
VERSION     DAJTRH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(2..94)
                     /locus_tag="OO339_001901"
     tRNA            complement(2..94)
                     /locus_tag="OO339_001901"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO339_001902"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO339_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5926552.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO339_001903"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO339_001903"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5926553.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO339_001904"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO339_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5926554.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO339_001905"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO339_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5926555.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO339_001906"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO339_001906"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5926556.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO339_001907"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO339_001907"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5926557.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO339_001908"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO339_001908"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO339_001909"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO339_001909"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5926558.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO339_001910"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO339_001910"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5926559.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO339_001911"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO339_001911"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5926560.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO339_001912"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO339_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5926561.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO339_001913"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO339_001913"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5926562.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO339_001914"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO339_001914"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5926563.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO339_001915"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO339_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5926564.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO339_001916"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO339_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5926565.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO339_001917"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO339_001917"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5926566.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO339_001918"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO339_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5926567.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO339_001919"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO339_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5926568.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO339_001920"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO339_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5926569.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO339_001921"
     CDS             complement(20900..21019)
                     /locus_tag="OO339_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5926570.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO339_001922"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO339_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5926571.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO339_001923"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO339_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5926572.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25517)
                     /gene="hycC"
                     /locus_tag="OO339_001924"
     CDS             complement(23682..25517)
                     /gene="hycC"
                     /locus_tag="OO339_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5926573.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLN
                     HSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFA
                     GEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKE
                     AENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITL
                     LLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPV
                     LKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25554..26165)
                     /gene="hycB"
                     /locus_tag="OO339_001925"
     CDS             complement(25554..26165)
                     /gene="hycB"
                     /locus_tag="OO339_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5926574.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26290..26751)
                     /gene="hycA"
                     /locus_tag="OO339_001926"
     CDS             complement(26290..26751)
                     /gene="hycA"
                     /locus_tag="OO339_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5926575.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26963..27313
                     /gene="hypA"
                     /locus_tag="OO339_001927"
     CDS             26963..27313
                     /gene="hypA"
                     /locus_tag="OO339_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5926576.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27317..28189
                     /gene="hypB"
                     /locus_tag="OO339_001928"
     CDS             27317..28189
                     /gene="hypB"
                     /locus_tag="OO339_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5926577.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28180..28452
                     /gene="hypC"
                     /locus_tag="OO339_001929"
     CDS             28180..28452
                     /gene="hypC"
                     /locus_tag="OO339_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5926578.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28452..29573
                     /gene="hypD"
                     /locus_tag="OO339_001930"
     CDS             28452..29573
                     /gene="hypD"
                     /locus_tag="OO339_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5926579.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29570..30580
                     /gene="hypE"
                     /locus_tag="OO339_001931"
     CDS             29570..30580
                     /gene="hypE"
                     /locus_tag="OO339_001931"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5926580.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30654..32732
                     /gene="flhA"
                     /locus_tag="OO339_001932"
     CDS             30654..32732
                     /gene="flhA"
                     /locus_tag="OO339_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5926581.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32769..33122)
                     /locus_tag="OO339_001933"
     CDS             complement(32769..33122)
                     /locus_tag="OO339_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5926582.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7809 a         9251 c         8630 g         7466 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagccccag ccccagcccc
    24661 agcaggataa tgccgatatt ttccagggtg tggtaagcca gcaggcgctg gatgttgtgt
    24721 tccatcaacg catacagacc accgacgaac gcggtgatca tgccgagcac cagcagcgcg
    24781 atcccccacc acagcggtgc attaccgccc agcagtgaca gggttaacat gcccagcagg
    24841 ccaattttca tgactaccgt agaaaacaac gcggcagctg gcgcagaggc gttagcatgt
    24901 gcctgcggca cccagccgtg cagcggaata atcccagcca gcaggccaaa gccaatcaca
    24961 ccgagcagcc agatatcgga accgagcggc agctgttgca tacgcatatc cagcaggcgc
    25021 agatccagcg tgccataacg ctgccacagc agccagcaag caatcaccag cagcagagtg
    25081 ccaagacgcc tcagcgcaaa ccacagtttg ccctctttgc tgttgctggt gaggaacacc
    25141 gcacacaagg ccatgacttc ggccattacc acgaacatgc cgaggttgct ggcgatgacg
    25201 gcgcagacgg cggcagccat caacatattg atctgcaagc cgttgcactt cacctgcgcg
    25261 tggcgatgcc agtcaatgtt atagaggctg acaaacagac cgcaaaggcc gagggtaatc
    25321 agccaaatcg cgttaagcgg ggagatttgc acatcgtagc ttaccagcga cagcgcaccg
    25381 ctcacgccaa ccgcgccagt cagtacagtg aagcccgcgg ctgccgtata cagactacca
    25441 accgcgccgc caattccggc tatccagcca cttagcgctt tttgaaaaga gaaatgccag
    25501 aacagcggcg gcgacaaacc atgccacgcc gctattgatc agggaaattg cgctcattta
    25561 gcctctccac tttgagcctg ctgaaacaag gtgagatcgc caaagtccgt gttaaaggtc
    25621 agctcacgct tacgtttgct gacgcgggcg atatcggtgt tatccaccag atgcagggct
    25681 ttagtcgggc acatccgcac acaggccgga ccttgttcat caaagctaca aaggtcacat
    25741 ttgacggcga tcgcgcgaat acctggcacc cagtcaagca atgtgctgac acgcgccgga
    25801 gcaggcggtg ccggtggcgc tttcggggta ttggcgtttg ccggaatatc cagcggacgg
    25861 ctgccggaaa attcaattgc gccaaacggg caggcgatgc cgcacagttt gcagcttacg
    25921 cacagacttt cattcaactg cacggcccca tcaacgcggg tgatggcgtt aaccgggcag
    25981 accaccgcgc agggtgcatc ttcacagtgg tgacagagct gcggcgcaga ttctttttca
    26041 ttgagcatca ctctcaggcg cggcattgat tgcaggccgt gctggcgatg cgtctctgaa
    26101 caggcggcct cacaggtgtg gcagccgata cagagcgtgg agtcggcaat tacaaaacga
    26161 ttcaccaggc attcctcagg tgattgtcat ttttgacgaa aacatgccga tgaaatgtca
    26221 ttttcgacac tcatcgacac gcccatcccc aaacaggcgt aacgcctgca aaacgggcaa
    26281 agcctcagct catgctgccg ggctttgtcc ctttaccagt tggcttaaat tcaccggcac
    26341 attgttttca atggcggtgt ataagctgtc gtaaacgcca gcgatttttt cgagatagtg
    26401 ttccagaacc tgttcgcgat cgcggatgtt gaccttgcca tcaatcatgc cgtcaatgct
    26461 cagttgcgcc tgcgcaatcc gctgtttgtc ttcgccatct tttgtttcga cgtaatactc
    26521 gatggtgtgg ctgttaaagc catccgccag ggtgacgtaa atacgaaaat gttcaaaaag
    26581 gacgaaacag agttctttgt ccggcgcgca gctcatggcg tgatgcaggc gcgctttcga
    26641 taacgtgatc ccctgaacca gcgaattgca gtagatgtgc cactggtcct gtaggcgacg
    26701 atgccgctgt gcgatgtaat cggctttctc gcttatttcc caaatagtca ttgtcaggtt
    26761 actcgtttaa cagagatgcc agctttaagc attttttgtg ccaactttta atttattgtt
    26821 attaaagaga tttttaagct aaagatgaat ttcgtcgccg tgtcgacgtg tcatttcgac
    26881 atcatcgaca ttattcaccg cagggataat caacactggc gcaattattg cttgtagctg
    26941 gcaatagtta atgggaggcg atatgcacga aataaccctc tgccaacggg cactggaatt
    27001 gatcgaacag caggccgcaa aacacggcgc aaaacgcgta actggggtct ggctcaaaat
    27061 tggcgcattt tcttgtgtcg aaaccagctc tcttgccttt tgttttgatc tggtttgccg
    27121 cggcagcgtg gcggaaggtt gtaaactgca cctcgaagaa caagaggccg aatgctggtg
    27181 tgaaacatgc caacagtatg tgacgctact gacccagcgc gtccgccgct gtccacagtg
    27241 tcatggtgac atgctacaga ttgtggcaga cgacggttta cagattcggc ggatagaaat
    27301 agaccaggag tgagcgatgt gtacaacatg cggttgcggt gaaggcaacc tgtatatcga
    27361 gggtgatgaa cataaccctc attccgcgtt tcgtagcgcg ccatttgccc cggcggcacg
    27421 cccgcagatg aaaatcaccg gcattaaagc gcctgaattt acccccagcc agactgaaga
    27481 aggcgacctg cattacggtc atggcgaagc gggcactcac acaccgggca tgagccagcg
    27541 tcggatgctg gaagtcgaaa ttgacgtgct ggacaaaaac aaccgtctgg ctgaacgcaa
    27601 ccgcgcgcgc tttgctgccc gcaagcaact ggtgctcaac ctggtttcca gccctggttc
    27661 cggtaaaacc accctgctga cggaaacctt aatgcgcctg aaagacagcg ttccgtgcgc
    27721 ggttattgaa ggcgaccagc aaaccgtgaa cgatgccgca cgcattcgcg ctaccggtac
    27781 accagcgatt caggtgaaca ccggtaaagg ctgccatctt gacgcacaga tgattgccga
    27841 cgccgcaccg cgtctgccac tggacgataa cggtattctg tttatcgaaa acgttggcaa
    27901 cctcgtatgc ccggccagct tcgatctcgg tgaaaaacac aaagtagcgg tgctttccgt
    27961 taccgaaggt gaagacaaac cgctgaaata tccgcatatg tttgccgccg cctcgctgat
    28021 gctgctcaac aaagttgacc tgttgccgta tctcaacttt gacgttgaga agtgcatcgc
    28081 ctgcgcccgc gaagtcaatc cagaaattga aatcatcctt atttccgcca ccagcggcga
    28141 agggatggac cagtggctga actggctgga gacacagcga tgtgcatagg cgttcccggc
    28201 cagatccgca ccattgacgg caaccaggcg aaagtcgacg tctgcggcat tcagcgcgat
    28261 gtcgatttaa cgttagtcgg cagctgcgat gaaaacggtc agccgcgcgt gggccagtgg
    28321 gtactggttc acgttggctt tgccatgagc gtaattaatg aagccgaagc acgcgacact
    28381 ctcgacgcct tacaaaacat gtttgacgtt gagccggatg tcggcgcgct gttgtatggc
    28441 gaggaaaaat aatgcgtttt gttgatgaat atcgcgcgcc ggaacaggtg atgcagttaa
    28501 ttgagcatct gcgcgaacgt gcttcacatc tctcttacac cgccgaacgc cctctgcgga
    28561 ttatggaagt gtgtggcggt catacccacg ccatttttaa attcggcctc gaccagttac
    28621 tgcctgaaaa cgttgagttt atccacggtc cgggttgccc ggtgtgcgta ctgccgatgg
    28681 gtagaatcga cacctgcgtg gagattgcca gccatccgga agtcatcttc tgtacctttg
    28741 gcgacgccat gcgcgtacca gggaaacagg gatcgctgtt gcaggcaaaa gcacgcggtg
    28801 ccgatgtgcg catcgtttac tcgccgatgg atgcgttgaa actggcgcag gagaatccaa
    28861 cccgcaaagt ggtgttcttc ggcttaggtt ttgaaaccac catgccgacc accgccatca
    28921 ctctgcaaca ggcgaaagcg cgtgatgtgc agaattttta cttcttctgc cagcacatta
    28981 cgcttatccc gacgctgcgc agtttgctgg aacagccgga taacggtatc gacgcgttcc
    29041 tcgcgccggg ccacgttagt atggttatcg gcactgatgc ctataatttt atcgccagcg
    29101 attttcatcg tccgctggtg gtcgctggtt tcgaacccct tgatctactg caaggcgtgg
    29161 tcatgctggt ggagcagaaa atagcggccc acagcaaggt agagaatcag tatcgtcggg
    29221 tggtaccgga tgccggtaac ctactggcgc aacaggcgat tgccgatgtg ttctgtgtca
    29281 acggcgacag cgaatggcgc ggcttaggcg tgattgaatc ttctggcgtg cacctgacgc
    29341 cggattatca acgattcgat gccgaagcac atttccgccc ggcaccgcag caggtctgcg
    29401 atgacccgcg cgcgcgttgt ggtgaggtat taacgggcaa atgtaagccg catcaatgcc
    29461 cgctgtttgg taacacctgt aatcctcaaa ccgcatttgg tgcactgatg gtctcctccg
    29521 aaggagcgtg cgccgcgtgg tatcagtatc gtcagcagga gagtgaagcg tgaataatat
    29581 ccaactcgcc cacggcagcg gcggccaggc gatgcagcaa ttaatcaaca gcctgtttat
    29641 ggaagccttt gccaacccgt ggctggcaga gcaggaagat caggcacgtc ttgatctggc
    29701 gcagctggta gcggaaggcg accgtctggc gttctccacc gacagttacg tcattgaccc
    29761 gctgttcttt cctggcggta atatcggcaa gctggcgatt tgcggcaccg cgaatgacgt
    29821 tgcgggcagt ggcgctattc cgcgctatct ctcctgtggc tttatcctcg aagaaggatt
    29881 gccgatggag acactgaaag ccgtagtgac cagcatggca gaaaccgccc gcacggcagg
    29941 cattgccatc gttactggcg atactaaagt ggtgcagcgc ggcgcggcag ataaactgtt
    30001 tatcaacacc gctggcatgg gcgcaattcc ggcgaatatt cactggggcg cacagacgct
    30061 aaccgcaggc gatgtattgc tggttagcgg tacactcggc gaccacgggg cgactatcct
    30121 taacctgcgt gagcagctgg ggctggatgg cgaactggtc agcgactgcg cggtgctgac
    30181 gccgcttatt cagacgctgc gtgacattcc cggcgtgaaa gcgctgcgtg atgccacccg
    30241 tggtggtgta aacgcggtgg ttcatgagtt cgcggcagcc tgcggttgtg gtattgaaat
    30301 ttctgaatca gcgctgccgg ttaaacctgc cgtgcgcggc gtttgcgaat tgctgggact
    30361 ggacgccctg aactttgcca acgaaggcaa actggtgatc gccgttgaac gcaacgcggc
    30421 agagcaagtg ctggcagcgt tacattccca tccactgggg aaagacgcgg cgctgattgg
    30481 tgaagtggtg gaacgtaaag gtgttcgtct tgccggtctg tatggcgtga aacgaaccct
    30541 cgatttacca cacgccgaac cgcttccgcg tatatgctaa taaaattcta aatctcctat
    30601 agttagtcaa tgaccttttg caccgctttg cggtgctttc ctggaagaac aaaatgtcat
    30661 atacaccgat gagtgatctc ggacagcaag ggttgttcga catcactcgg acactattgc
    30721 agcagcccga tctggcctcg ctgtgtgagg ctctttcgca actggtaaag cgttctgcgc
    30781 tcgccgacaa cgcggctatt gtgttgtggc aagcgcagac tcaacgtgcg tcttattacg
    30841 cgtcgcgtga aaaagacacc cccattaaat atgaagacga aactgttctg gcacacggtc
    30901 cggtacgcag cattttgtcg cgccctgata cgctgcattg cagttacgaa gaattttgtg
    30961 aaacctggcc gcagctggcc gcaggtgggc tataccccaa atttggtcac tattgcctga
    31021 tgccactggc ggcgggaggg catatttttg gtggctgtga atttattcgt tatgacgagc
    31081 gtccctggag cgaaaaagag ttcaatcgtc tgcaaacatt tacgcagatc gtttctgtcg
    31141 tcaccgaaca aatccagagc cgcgtcgtca acaatgtcga ctatgagttg ttatgccggg
    31201 agcgcgataa cttccgcatc ctggttgcca ttaccaacgc ggtgctttcc cgcctggata
    31261 tggacgaact ggtcagcgaa gtcgccaaag aaatccatta ctatttcgac attgacgata
    31321 tcagcattgt cttacgcagc caccgtaaaa acaaactcaa catctactcc actcactatc
    31381 ttgataaaca acatcccgcc cacgaacaga gcgaagtcga tgaagccgga accctcaccg
    31441 aacgcgtgtt caaaagtaaa gagatgctgc tgatcaatct ctacgagcgg gacgatttag
    31501 ccccctatga acgcatgttg ttcgacacct ggggcaacca gattcaaacc ttgtgcctgt
    31561 taccgctgat gtctggcgac accatgctgg gcgtgctgaa actggcgcaa tgcgaagaga
    31621 aagtgtttac cactaccaat ctgaatttgc tgcgccagat tgccgaacgt gtggcaatcg
    31681 ctgtcgataa cgccctcgcc tatcaggaaa tccatcgtct gaaagaacgg ctggttgatg
    31741 aaaacctcgc cctgaccgag cagctcaaca atgttgatag tgaatttggc gagattattg
    31801 gccgcagcga agccatgtac agcgtgctta aacaagttga aatggtggcg caaagtgaca
    31861 gtaccgtgct gatcctcggt gaaactggct cgggtaaaga gctgattgcc cgtgcgatcc
    31921 ataatctcag tgggcgtaat aatcgccgca tggtcaaaat gaactgcgcg gcgatgcctg
    31981 ccggattgct ggaaagcgat ctgtttggtc atgagcgtgg ggcttttacc ggtgccaacg
    32041 cccagcgcat tggtcgtttt gaactggcgg ataaaagctc tctgttcctc gacgaagtgg
    32101 gcgatatgcc actggagtta cagccgaagt tgctgcgtgt attgcaggaa caggagtttg
    32161 aacgtctcgg cagcaacaaa atcattcaga cggacgtacg tttaattgcc gcgactaacc
    32221 gcgatctgaa aaaaatggtc gccgaccgag agttccgtag cgatctctat taccgcctga
    32281 acgtattccc gattcacctg ccgccactac gcgagcgtcc ggaagatatt ccgctgctgg
    32341 cgaaagcctt taccttcaaa attgcccgtc gtctggggcg caatatcgac agcattcctg
    32401 ccgagacgct gcgcaccttg agcaacatgg agtggccggg taacgtacgc gaactggaaa
    32461 acgtcattga gcgcgcagta ttgctaacac gcggcaacgt gctgcagctg tcattgccag
    32521 atattgcttt accggagcct gaaacgccgc ctgccgcaac ggttgtcgcc caggagggcg
    32581 aagatgaata tcagttgatt gtgcgcgtgc tgaaagaaac caacagcgtg gttgccgggc
    32641 ctaaaggcgc tgcgcaacgt ctggggctga aacgcacgac cctgctgtca cggatgaagc
    32701 ggctgggaat tgataaatcg gcattgattt aactgcaaat tgccggacag atctgcctgt
    32761 ccggcatact attcacgagg ttttttcgga cgatattttt ccggcagttc tggcaccgga
    32821 cgcttgtcat cgatgagatg acgcacggtt aagatcggat gacgccacag cattctcggc
    32881 ccggcccaac gcataatctg tttcatctct tcacgctttg caggctggta acagtgcacc
    32941 ggacactgct tacaggctgg tttctcttcg ccgaacacac atttatccag ccgcttttgc
    33001 gcgtaaacaa acaacgcctc gtaatgctcc ggctccgctg acgcctgcgg gcatttcgct
    33061 tgataaagat cgatcatttt tttaatcgtc agtttttcac gagagatacg cttgccggac
    33121 atgctgcctc cacctcatta agatgcattt atatta
//
LOCUS       DAJTRH010000038        31540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTRH010000038 DAJTRH010000000
VERSION     DAJTRH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO339_001934"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO339_001934"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5926583.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO339_001935"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO339_001935"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5926584.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO339_001936"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO339_001936"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5926585.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO339_001937"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO339_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5926586.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO339_001938"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO339_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5926587.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO339_001939"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO339_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5926588.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO339_001940"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO339_001940"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5926589.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO339_001941"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO339_001941"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5926590.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO339_001942"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO339_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5926591.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO339_001943"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO339_001943"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5926592.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO339_001944"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO339_001944"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5926593.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO339_001945"
     CDS             8212..9075
                     /locus_tag="OO339_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5926594.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO339_001946"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO339_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5926595.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO339_001947"
     CDS             10411..11028
                     /locus_tag="OO339_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5926596.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO339_001948"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO339_001948"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5926597.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO339_001949"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO339_001949"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5926598.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO339_001950"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO339_001950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5926599.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO339_001951"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO339_001951"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5926600.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO339_001952"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO339_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5926601.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO339_001953"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO339_001953"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5926602.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO339_001954"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO339_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5926603.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO339_001955"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO339_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5926604.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO339_001956"
     CDS             21859..23568
                     /locus_tag="OO339_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5926605.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO339_001957"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO339_001957"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5926606.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO339_001958"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO339_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO339_001959"
     tRNA            27625..27719
                     /locus_tag="OO339_001959"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO339_001960"
     CDS             27991..29202
                     /locus_tag="OO339_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5926607.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO339_001961"
     CDS             29614..30657
                     /locus_tag="OO339_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5926608.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..>31540
                     /locus_tag="OO339_001962"
     CDS             31172..>31540
                     /locus_tag="OO339_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCR5926609.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLP"
BASE COUNT         7901 a         8318 c         7822 g         7499 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca
//
LOCUS       DAJTRH010000039        30483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTRH010000039 DAJTRH010000000
VERSION     DAJTRH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            393..2051
                     /locus_tag="OO339_001963"
     CDS             393..2051
                     /locus_tag="OO339_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5926610.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2048..3004)
                     /locus_tag="OO339_001964"
     CDS             complement(2048..3004)
                     /locus_tag="OO339_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5926611.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3155..4270
                     /gene="macA"
                     /locus_tag="OO339_001965"
     CDS             3155..4270
                     /gene="macA"
                     /locus_tag="OO339_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5926612.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4267..6213
                     /gene="macB"
                     /locus_tag="OO339_001966"
     CDS             4267..6213
                     /gene="macB"
                     /locus_tag="OO339_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5926613.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="OO339_001967"
     CDS             complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="OO339_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5926614.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6833..7153
                     /gene="clpS"
                     /locus_tag="OO339_001968"
     CDS             6833..7153
                     /gene="clpS"
                     /locus_tag="OO339_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5926615.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7184..9460
                     /gene="clpA"
                     /locus_tag="OO339_001969"
     CDS             7184..9460
                     /gene="clpA"
                     /locus_tag="OO339_001969"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5926616.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9863..9950)
                     /locus_tag="OO339_001970"
     tRNA            complement(9863..9950)
                     /locus_tag="OO339_001970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9914..9916),aa:Ser,seq:gga)
     gene            complement(10204..10422)
                     /gene="infA"
                     /locus_tag="OO339_001971"
     CDS             complement(10204..10422)
                     /gene="infA"
                     /locus_tag="OO339_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5926617.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10707..11411)
                     /gene="aat"
                     /locus_tag="OO339_001972"
     CDS             complement(10707..11411)
                     /gene="aat"
                     /locus_tag="OO339_001972"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5926618.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11453..13174)
                     /gene="cydC"
                     /locus_tag="OO339_001973"
     CDS             complement(11453..13174)
                     /gene="cydC"
                     /locus_tag="OO339_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5926619.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13175..14941)
                     /gene="cydD"
                     /locus_tag="OO339_001974"
     CDS             complement(13175..14941)
                     /gene="cydD"
                     /locus_tag="OO339_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5926620.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15064..16029)
                     /gene="trxB"
                     /locus_tag="OO339_001975"
     CDS             complement(15064..16029)
                     /gene="trxB"
                     /locus_tag="OO339_001975"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5926621.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16573..17067
                     /gene="lrp"
                     /locus_tag="OO339_001976"
     CDS             16573..17067
                     /gene="lrp"
                     /locus_tag="OO339_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5926622.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17202..21230
                     /gene="ftsK"
                     /locus_tag="OO339_001977"
     CDS             17202..21230
                     /gene="ftsK"
                     /locus_tag="OO339_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5926623.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21385..21996
                     /gene="lolA"
                     /locus_tag="OO339_001978"
     CDS             21385..21996
                     /gene="lolA"
                     /locus_tag="OO339_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5926624.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22007..23350
                     /gene="rarA"
                     /locus_tag="OO339_001979"
                     /gene_synonym="mgsA"
     CDS             22007..23350
                     /gene="rarA"
                     /locus_tag="OO339_001979"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5926625.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23441..24733
                     /gene="serS"
                     /locus_tag="OO339_001980"
     CDS             23441..24733
                     /gene="serS"
                     /locus_tag="OO339_001980"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5926626.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24973..27416
                     /gene="dmsA"
                     /locus_tag="OO339_001981"
                     /pseudo
     CDS             24973..27416
                     /gene="dmsA"
                     /locus_tag="OO339_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27427..28044
                     /gene="dmsB"
                     /locus_tag="OO339_001982"
     CDS             27427..28044
                     /gene="dmsB"
                     /locus_tag="OO339_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5926627.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28046..28837
                     /locus_tag="OO339_001983"
     CDS             28046..28837
                     /locus_tag="OO339_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5926628.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28873..29499)
                     /locus_tag="OO339_001984"
     CDS             complement(28873..29499)
                     /locus_tag="OO339_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5926629.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29814..>30483
                     /locus_tag="OO339_001985"
     CDS             29814..>30483
                     /locus_tag="OO339_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5926630.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLM"
BASE COUNT         7549 a         7488 c         8202 g         7244 t
ORIGIN      
        1 aatatggtcg ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt
       61 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
      121 acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt
      181 taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt
      241 tgcgggctta tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg
      301 ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg
      361 taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg
      421 gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg
      481 agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat
      541 cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc
      601 gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg
      661 ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta
      721 ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt
      781 ttctcgataa agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc
      841 tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta
      901 acggcacggt gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg
      961 cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt
     1021 ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat
     1081 atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca
     1141 tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt
     1201 ttgctacttt attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat
     1261 tgctaattga agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc
     1321 tgttgaatct tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt
     1381 taactccagt ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc
     1441 gtctggggcc aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt
     1501 ttaatcgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa
     1561 cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca
     1621 aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg
     1681 ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg
     1741 tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg
     1801 cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg
     1861 cgcaaatccc ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc
     1921 gctcttccaa acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg
     1981 actccgtacc aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc
     2041 gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat
     2101 agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta
     2161 tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga
     2221 acgcgttgta atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa
     2281 tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac
     2341 aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct
     2401 ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc
     2461 ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt
     2521 tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa
     2581 gcgtgaactg ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca
     2641 aatataagct gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta
     2701 acgcctccag caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca
     2761 caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt
     2821 tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa
     2881 aactacagcg atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa
     2941 gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat
     3001 tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat
     3061 caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg
     3121 acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa
     3181 gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct
     3241 taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag
     3301 cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag
     3361 cggtcagttg aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt
     3421 aggggtcatt gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat
     3481 ggagctacgc gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta
     3541 ttcccgtcag caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc
     3601 cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg
     3661 caatcaggcc tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc
     3721 gatggctggg gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca
     3781 gcaagcaccg aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt
     3841 ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg
     3901 cgatccactg acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt
     3961 aaacgacgct attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg
     4021 gctggatatg accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat
     4081 ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg
     4141 taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga
     4201 gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc
     4261 tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat
     4321 gagcaggttg aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg
     4381 attgttggcg cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat
     4441 aaggccacca gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat
     4501 gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt
     4561 ccgcatttaa ccgtcgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg
     4621 aaacagcgac tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca
     4681 gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca
     4741 ttgatgaacg gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat
     4801 tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt
     4861 atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac
     4921 ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag
     4981 cctgttgtca atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg
     5041 acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg
     5101 attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa
     5161 atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa
     5221 gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc
     5281 atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg
     5341 cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat
     5401 gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt
     5461 gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat
     5521 gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa
     5581 gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg
     5641 atgtccgggc gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa
     5701 ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt
     5761 aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca
     5821 cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt
     5881 ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc
     5941 atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg
     6001 gtttgcctgg tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg
     6061 cagcttttct tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt
     6121 ctctgctcga cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga
     6181 ctggatccag tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgatc
     6241 gggctggcat tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc
     6301 ttctacttcg acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc
     6361 aaactgaacg gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata
     6421 atgagcgaaa atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt
     6481 gttgaaccac ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa
     6541 gtaagatgga ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa
     6601 atacaattta gagaattagg gcgagccgtc aagcatttga caggggccag ggggcaggta
     6661 tgaatcaaaa atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg
     6721 ccaacgatag atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt
     6781 gtcagcggca gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa
     6841 aacgaacgac tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa
     6901 accgccatct atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt
     6961 tattgacgtg ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct
     7021 cgctgttcac taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac
     7081 caaagtggcg atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct
     7141 agaaaaagcc tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact
     7201 ggaactcagt ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac
     7261 cgtcgagcac ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc
     7321 gtgttctgtg gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac
     7381 acccgttctg cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg
     7441 tgtactgcaa cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc
     7501 aaacgttctg gtcgctatct tcagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa
     7561 acatgaagtc agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga
     7621 gccgacacag tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga
     7681 ggaacgtatg gagaatttca cgacgaacct gaaccagctt gcgcgtgtgg gcggaatcga
     7741 cccactaatt ggtcgtgaga aagagctgga gcgtgctatt caggttctct gccgtcgccg
     7801 taaaaacaac ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg
     7861 tcttgcctgg cgaattgttc agggtgatgt gccggaagtg atggctgact gtacgattta
     7921 ctctctcgat atcggttctc tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg
     7981 ttttaaagcg ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga
     8041 gatccacacc attatcggtg cgggtgcagc gtctggtggt caggtcgatg cggctaacct
     8101 aatcaaaccg ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga
     8161 gttcagcaac attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat
     8221 tactgaaccg tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga
     8281 agcgcaccac gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt
     8341 gaaatacatt aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcagg
     8401 cgctcgcgca cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat
     8461 tgagtccgtg gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg
     8521 cgataccctg aaaaaactca gcgatcgcct gaaaatgctg gtcttcggcc aggataaagc
     8581 cattgaggcg ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa
     8641 accggtcggt tcgttcctgt ttgccggtcc taccggggtc gggaaaacag aggtgacggt
     8701 acagctttcg aaagcgttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga
     8761 acgccatacc gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgatcaggg
     8821 cggtttgctg accgatgcgg taatcaagca tccacatgcg gtgctgctgc tggacgaaat
     8881 cgagaaagcg catccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct
     8941 gaccgataac aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccactaa
     9001 cgccggggta cgggaaactg agcgcaaatc cattggtctt atccaccagg ataacagcac
     9061 cgatgcgatg gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa
     9121 cattatctgg ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat
     9181 cgtcgagttg caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc
     9241 gcgtaactgg ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg
     9301 tgtcatccag gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt
     9361 ggacggcggt caggtgacgg ttgcgctgga taaagagaaa aatgagctga cttacggatt
     9421 ccagagtgca caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg
     9481 gtgaagtccg taatctcgtc aggggttacg gactttttat ttatggggtg gaacggtgaa
     9541 aaccctattt ttggaggtga aggtaagttg ttgataatta gtgctgctgg aaggtaagga
     9601 taaaaaaggg tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt
     9661 gtagtatttg agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc
     9721 tttgcgaacg cgttcactgc cgtacaggca gcttagaaat tcacaggtaa catactccac
     9781 ccgcccacca tgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt
     9841 cgtacgagct cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc
     9901 cgtatacaca ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt
     9961 gctaccaaac ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc
    10021 aagaagaatt ttaatgataa ttattgtttg ctcatactgt aaacaacttg tgcagtatat
    10081 ctacatcgag acaagttatg gacttatact tccaaagtac ttcatacata tcacaaaata
    10141 aaaaggccgg ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa
    10201 caatcagcga ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac
    10261 agtcactttg tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg
    10321 tgcagtaacc acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc
    10381 aagaacggta ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc
    10441 actaccgtaa tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc
    10501 gcgctaaatc gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca
    10561 ccaaaagcgc aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa
    10621 aattccttat cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac
    10681 tctggggaga aatgtgccga aaacattcat tcttgtggtg aaaacaagca ccgtggtacc
    10741 cagaaattat tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg
    10801 cggggaattt cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata
    10861 agcttaccgc catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca
    10921 ttttccatcc ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac
    10981 ataccgccga caagctcatc ttcacgccag acttcaatgg agtgggcatg accgagttcg
    11041 tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg
    11101 ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag
    11161 cgtttatgaa atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg
    11221 cggggatcgg gcgaccacca gaggatgggg tcgcctggag aaaaccacgg aaaaatacca
    11281 cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg
    11341 ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga
    11401 accaagcgca tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac
    11461 cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag ttctgcgtga gtaccttgct
    11521 caataatttg cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc
    11581 gatgggtgac cattaacacc gttttctcac gcatcatttt tgcaagcaat tcaaggatct
    11641 ggctttcggt tgtggcatct aagccttcgg taggttcatc cagcaacacc agtggcgcat
    11701 catgtaacag cgtacgggcg atagccagac ggcgcagttc accaccggag agctggcgtc
    11761 cgccttcacc taaccaactg ttgagacctg catcctcgag cagcttctcc agaccaacgc
    11821 gacgcaagat ctccgacaga gcctcatcac tactgccagg cgaggcgagt aacagattat
    11881 cacgcagtgt ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta
    11941 gagccgcttc attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg
    12001 ggtcccatgc gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga
    12061 gaatcgctat atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg
    12121 attgctccgg ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga tcggcaacac
    12181 gagtttgggt atcagggaag gtgacctccg gtttttgatc cgttaagtca gagatacgta
    12241 cggcagaggc aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt
    12301 caaacgcggc taacgcacaa aagacaaaca gggcaattaa tgcgccgggt tgagcattgc
    12361 cgccaacgcc gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagca
    12421 gcattatcgc ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt
    12481 gaatttctgt attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct
    12541 cagcttgccc ttgcagccag gccgtcagtt gttggcgata ctgtccgcga agatgagtca
    12601 gattttgccc ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca
    12661 gtaacataac gctgcccagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg
    12721 tcaccaccat aatcaccaca aaagcgccca ccagcggcga gataacgcgc aggtaaagat
    12781 gatcgagcgt atcaacatcc gccaccacgc ggttgagcaa ttcgccctga cgatagcgcg
    12841 ccagtccggc aggggagagg ggcaacaatt tgctgaaggt gtaaatgcgc agatgctgca
    12901 acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg
    12961 tgattgcggc accacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg
    13021 caacccccgc aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac
    13081 tggcgagcag cgtcacaatt gccagcacaa taccaagact taacatccat ttatgacgtt
    13141 tatacagtgc cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca
    13201 gtaatgtggc gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa
    13261 tccggccatc ctgcataacc caaatgacat cccagtcagc aagatcttct aactggtggg
    13321 tgaccattaa cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt
    13381 cactgtgagc atcaaggctg gcagcgggtt catccaacag taatagcgaa cagggattta
    13441 gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt
    13501 cgccaacagg tgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt
    13561 tatccagcgc tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt agtacgttat
    13621 cccgcaattt tgctgccggt aattgtgggt tttgcccaac ccaggagaga tgtttacgcc
    13681 atgattctgg tgataaatca cgtaattcta tcccgttgat tcgtaatgat ccctgatatg
    13741 agagaaaacc agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg cggccaacca
    13801 acactgcacg ttggcctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt
    13861 ccggcgacgt gataaacaga tcctcggcct caatggtcag cggatcggtc aatgctaatt
    13921 ccgcctcacc gcgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag
    13981 ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgt aatggctgga
    14041 aaaactctgg cgcaaggatc agggccagaa aacctgcagc cagcgtcacc ccagtatcgt
    14101 agtgaccaaa atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa
    14161 ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaacgccagt cgtagcactt
    14221 ccattgtccg ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac
    14281 cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta
    14341 agcgagcaag agcgagaaag ttacgccggt tagcatcggc agcccccatt ccaaccagcg
    14401 ccataaacaa cggaattagc ggtgctgtgc ccagcagaat gagcgccgca gcccagttag
    14461 aggggaagat cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca
    14521 gatagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccagc gtcgcccagc
    14581 tccccgcagg tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct
    14641 gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc
    14701 agaccaccca tgcgcgcaat acaaaggtca gaaccagtaa cgtaaaggga agcagcaggg
    14761 cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct
    14821 gggcaatgat caatatgccg cttacaaagc ccagcagacg agaaatattc agccaacgtt
    14881 gggagatgac gctttgctgt tttaaccagc gggttaactc tttttgacga gatttattca
    14941 ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacggggaaa
    15001 aaataaaggc gacccatagt cgcaaggtgt cgccttcttt acttttgtta ctgatttgta
    15061 aaattatttt gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca
    15121 gccagtaccg gccgaagtaa tggcctggcg ataaatgtga tccatcacgt cgcctgcggc
    15181 aaagacgcca ggaatgctgg tctgggtggc attaccatga atacccgact gtactttgat
    15241 gtagccgttt tccagttcca gctgcccttc gaaaatcgca gtattcgggc tgtgaccgat
    15301 agcaacaaac agaccggcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg
    15361 cagacgaacg ccagtgacac ccatttgatc gccggtcact tcttccagcg tacggttggt
    15421 gtgcagaatg atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc
    15481 cgcgcggaaa ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata
    15541 cagcgcctct tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata
    15601 gaagaaaccg tcgcaggttg cacaagcaga aaccccacgg cctttaaagg cttcttcaga
    15661 gggcaggccg agatagcgtg cagaagctcc ggtggcaata atcagcgcgt cgcaagtgta
    15721 ttcgccgtta tcgccattca gacggaacgg acggttttgc agatccacct tgttgatatg
    15781 atcaaaaatg atctcagttt caaacttggt ggcatgttcg tgcatgcgcg ccattaataa
    15841 cggaccggtc agatcgtttg gatcaccagg ccagttttcc acttccgtgg tggtggtcag
    15901 ttggccgcct ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgccgcgta
    15961 gacagcagcg gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt
    16021 cgtgcccatg agatccccat agttgttggc agacaatggg caggattgta gggaatttac
    16081 agacgtaaaa aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata
    16141 agcaggtcaa attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc
    16201 cgattaaatg atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc
    16261 gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaca
    16321 gtattcttat atgcgcataa ccatgtatgt aaataccatg tttaccgtgc tagtgaaatc
    16381 tacgtatggc gtggacagac gccattcgtg atgtcgatag ctgccacaag gcaacggtct
    16441 tctcaccgta gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac
    16501 gggtagcgac tctgaacagt gatgtttcag ggtcagacag gagtagggaa ggaatacaga
    16561 gagacaataa taatggtaga tagcaagaag cgccctggca aagatctcga ccgtatcgat
    16621 cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa
    16681 cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg
    16741 tttattcagg gctatacggc gctgcttaac ccccattatc tggatgcatc acttctggta
    16801 ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct
    16861 gtacaaaaac ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg
    16921 ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg
    16981 cgtctgcctg gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt
    17041 aatcgtctgg ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat
    17101 tacactcctg ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt
    17161 tagcatcggg caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg
    17221 aagacaaaga agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcattgc
    17281 tgatccttat tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt
    17341 cggaccccag ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc
    17401 ccggtgcgtg gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc
    17461 ccgtcattat tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca
    17521 ttgattattt tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct
    17581 gtggtctggc ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg
    17641 gcagcttact aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc
    17701 tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg
    17761 ctgaaaaact cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc
    17821 gcgatgatac ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa
    17881 atcacggcaa acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc
    17941 gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt
    18001 tctccggtaa gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg
    18061 ccgacccgga cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat
    18121 acgatccatt attaaacagt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta
    18181 ccacggcgac acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg
    18241 cctctgttga tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac
    18301 aaacgggaga gccggttatt gctcctgcac cggaaggtta cccacagcag tcacaatatg
    18361 cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc agtacagccg cagcagccgt
    18421 attatgcacc tgcagctgaa caacctgcgc aacagccgta ttatgcccct gcgccagaac
    18481 aaccggtggc aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt
    18541 ctacatacca gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac
    18601 agccgcaacc cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa
    18661 aacccgcgcg tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac
    18721 gtgaacaact tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga
    18781 tcaaatcttc gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg
    18841 ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa
    18901 ccgttgccgc gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag
    18961 aggggattgg cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat
    19021 tggcgtctta cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag
    19081 cccagcgcaa tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc
    19141 agcaggatga actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt
    19201 atcaacatga tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg
    19261 ctcgtcagtt tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga
    19321 atccgttctc gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc
    19381 cacacgaacc gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg
    19441 caccacagca gcagtatcag cagccacaac agccggttgc gccacagcag cagtatcagc
    19501 agccacaaca gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc
    19561 cacagcagca atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcaacagc
    19621 cgcaacaacc ggttgcaccg cagccgcagt atcagcagcc acaacagcca gttgcgccac
    19681 agccgcagga taccctgctt catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc
    19741 acaaaccgac gacgccactg ccttctctgg atttgctgac accgccgccg agcgaagtgg
    19801 agccggtaga tacctttgcg cttgaacaaa tggcgcgtct ggtggaagcg cgtctggctg
    19861 atttccgtat taaagccgat gtcgttaatt actctccggg gccagttatc actcgctttg
    19921 aacttaacct ggctcctggc gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg
    19981 cccgttcact ttcgacggtg gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg
    20041 taggtctgga gttaccaaat aaaaaacgac aaaccgttta tctgcgcgaa gttttggata
    20101 acgccaaatt ccgcgataat ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg
    20161 gtgagccggt ggttgccgat ctggcgaaaa tgccgcactt gttggttgcg gggactaccg
    20221 gttccggtaa atctgtcggt gtgaacgcga tgatcctgag catgctttat aaagcacagc
    20281 cagaagatgt gcgtttcatc atgatcgacc cgaaaatgct ggagctttcg gtttatgaag
    20341 gcattccgca cctgttaacg gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc
    20401 gctggtgtgt taacgagatg gagcgtcggt ataaactgat gtctgcgctg ggtgtgcgta
    20461 atctggcggg ttataacgaa aaaattgctg aagccgatcg catgatgcgt ccgattccag
    20521 acccgtactg gaagccgggt gacagtatgg atgcccagca tccggtgctg aaaaaagaac
    20581 catacattgt cgtgttggtt gacgaatttg ccgacctgat gatgacggta ggtaaaaaag
    20641 tggaagagct gatagctcgt ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac
    20701 tggcaactca gcgtccatcg gttgatgtta ttactggtct gattaaagcg aatattccga
    20761 cccgtatcgc ctttaccgta tccagtaaga ttgactcacg taccattctt gatcaggctg
    20821 gcgcggaatc actgctgggt atgggggata tgctctactc tgggccgaac tccacgttgc
    20881 cggtacgtgt ccatggtgct tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact
    20941 ggaaagcgcg tggtcgccca cagtatgttg atggcatcac ctccgacagc gaaagcgaag
    21001 gtggtgcggg tggtttcgat ggcgctgaag aactggatcc gttgttcgat caggcggtgc
    21061 agtttgtcac tgaaaaacgc aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg
    21121 gttataaccg tgcagcgcgt attatcgaac agatggaagc gcaggggatt gtcagcgaac
    21181 aggggcacaa cggtaatcgt gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt
    21241 gccggataag gcgcggtagc gtcgcatccg gcactctatc aactgaaaat tcagtatttt
    21301 cttctttcct caagctgatt attagcctgg aatagagagt agagggaact cccgatcggg
    21361 agtgacgtaa tttgaggaat aatgatgaaa aaaattgcta tcacctgtgc attactctca
    21421 agcttagtag caagcagcgt ttgggctgat gccgcaagcg atctgaaaag ccgcctggat
    21481 aaagtcagca gcttccacgc cagcttcaca caaaaagtga ctgacggtag cggcgcggcg
    21541 gtgcaggaag gtcagggcga tctgtgggtg aaacgtccaa acttattcaa ctggcatatg
    21601 acacaacctg atgaaagcat tctggtttct gacggtaaaa cactgtggtt ctataacccg
    21661 ttcgttgagc aagctacggc aacctggctg aaagatgcca ccggtaatac gccgtttatg
    21721 ctgattgccc gcaaccagtc cagcgactgg cagcagtaca atatcaaaca gaatggcgat
    21781 gactttgtcc tgacgccgaa agccagcaat ggcaatctga agcagttcac cattaacgtg
    21841 ggacgtgatg gcacaatcca tcagtttagc gcggtggagc aggacgatca gcgcagcagt
    21901 tatcagctga aatcccagca aaatggggct gtggatgcag cgaaatttac cttcaccccg
    21961 ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc acctgagtga gcaatctgtc
    22021 gctcgatttt tcggataata cttttcaacc tctggccgcg cgtatgcggc cagaaaattt
    22081 agcacagtat atcggtcagc aacatttgct ggctgcgggg aagccgttgc cgcgcgctat
    22141 cgaagccggg catttgcatt ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac
    22201 cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac
    22261 ctctggcgtg aaagagattc gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg
    22321 tcgccgcact attctttttg ttgacgaagt tcaccgtttc aacaaaagcc agcaggatgc
    22381 atttctgcca catattgaag acggcaccat cacttttatt ggcgcaacca ctgaaaaccc
    22441 gtcgtttgag cttaattcgg cactgctttc ccgtgcccgt gtttatctgt tgaaatccct
    22501 gagtacagag gatattgagc aagtactaac tcaggcgatg gaagacaaaa cccgtggcta
    22561 tggtggtcag gatattgttc tgccagatga tacacgacgt gccattgcag aactggtgaa
    22621 tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg gcggatatgg ccgaagtcta
    22681 tgatagcggt aagcgggtcc tgaagcctga attactgacc gaaatcgccg gtgaacgtag
    22741 cgcccgcttt gataacaaag gcgatcgctt ttacgatcta atttccgcac tgcataagtc
    22801 ggtacgtggt agcgcacccg atgcggcgct gtactggtat gcgcgaatta ttaccgctgg
    22861 tggcgatccg ttatatgtcg cgcgtcgctg tctggcgatt gcgtctgaag acgtcggtaa
    22921 tgccgatcca cgggcgatgc aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg
    22981 cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt tacctggcct gcgcgccaaa
    23041 aagcaacgct gtctacactg cgtttaaagc cgcgctggcc gatgctcgcg aacgcccgga
    23101 ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta
    23161 cgggcaggaa tatcgttatg ctcatgatga agcaaacgct tatgctgccg gtgaggttta
    23221 cttcccgccg gaaatagcac aaacacgcta ttatttcccg acaaacaggg gccttgaagg
    23281 caagattggt gaaaagctcg cctggctggc tgaacaggat caaaatagcc ccataaaacg
    23341 ctaccgttaa tgttatcgtt gcggtaatgt tgttactgta tccctgtggt cgcaggctgt
    23401 ggccacatct cccatttaat tcgataagca caggataagc atgctcgatc ccaatctgct
    23461 gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc cggggcttta agctggatgt
    23521 agataagctg ggcgctcttg aagagcgtcg taaagtattg caggtcaaaa cggaaaacct
    23581 gcaagcggag cgtaactccc gatcgaaatc cattggccag gcgaaagcgc gcggggaaga
    23641 tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa gagctggatg cagcaaaagc
    23701 cgagctggat gctttacagg ctgaaattcg cgatatcgcg ctgaccatcc ctaacctgcc
    23761 tgcagatgaa gtgccggtag gtaaagacga aaatgacaac gttgaagtca gccgctgggg
    23821 taccccgcgt gagtttgact ttgaagttcg tgaccacgtg acgctgggtg aaatgcactc
    23881 tggcctcgac tttgcagctg cagttaagct gactggttcc cgctttgtgg taatgaaagg
    23941 gcagattgct cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc ataccgaaca
    24001 gcatggctac agtgagaact atgttccgta cctggttaac caggacacgc tgtacggtac
    24061 gggtcagctg ccgaaatttg ctggcgatct gttccatact cgtccgctgg aagaagaagc
    24121 agacaccagt aactatgcgc tgatcccaac ggcagaagtt ccgctgacta acctggtgcg
    24181 cggtgaaatc atcgatgaag atgatctgcc aattaagatg accgcccaca ccccatgctt
    24241 ccgttctgaa gccggttcat atggtcgtga cacccgtggt ctgatccgta tgcaccagtt
    24301 cgacaaagtt gaaatggtgc agatcgtgcg cccagaagac tcaatggcgg cgctggaaga
    24361 gatgactggt catgcagaaa aagtcctgca gttgctgggc ctgccgtacc gtaaaatcat
    24421 cctttgcact ggcgacatgg gctttggcgc ttgcaaaact tacgacctgg aagtatggat
    24481 cccggcacag aacacctacc gtgagatctc ttcctgctcc aacgtttggg atttccaggc
    24541 acgtcgtatg caggcacgtt gccgcagcaa gtcggacaag aaaacccgtc tggttcatac
    24601 cctgaacggt tctggtctgg ctgttggtcg tacgctggtt gcagtaatgg aaaactatca
    24661 gcaggctgat ggtcgtattg aagtaccaga agttctgcgt ccgtatatga acggactgga
    24721 atatattggc taatacccaa tttttctgaa tctaaaaagc gcctttaggg cgcttttttt
    24781 gtctcccttt gataccgaac aataattact cctcacttac acgtaatact actttcgagt
    24841 gaaaatctac ctatctcttt gattttcaaa ttattctatg tatacaagcc tatatagcga
    24901 gctgctatag aaataattac acaatacggt ttgttactgg aatcaatcgt gatcaagctt
    24961 gagtgagcca ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggagagtcgc
    25021 cgtggtttgg taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca
    25081 ttacctttta gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa
    25141 aaggttatct ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac
    25201 gtcgtggacg gtgaaatcaa atatgtcgaa acgacaatac cggcgatgac aattacgacg
    25261 gcctacacta ggttcgcgcc tgcctgcgtg ggcgttccat gcgtcaccgt gtctacaatc
    25321 cggaccgcct gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc
    25381 gcattagctg ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag
    25441 agtacggcaa cgagtctatc tatctgaact atggcaccgg tacgctgggc ggcaccatga
    25501 cccgctcctg gccgccggga aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt
    25561 atcttaacca ttacggtgac tactcttccg cgcaaattgc ggaaggtttg aaccatacct
    25621 acggcggctg ggcagatggt aacagcccgt cggatatcga aaacagtaaa ttggtagtgc
    25681 tgtttggtaa taacccaggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg
    25741 aacaggcacg ccagaaatct aatgctcgca tgatcatcat cgatccgcgc tataccgaca
    25801 ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc aggaacagat gccgcactgg
    25861 ttaacggtct ggcgtacgtc atgatcacta aaaacctggt ggatcaggca ttcctcgata
    25921 aatattgcgt tggctatgac gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt
    25981 ataaagctta tattctgggt gaagggccag atggcgtggc taaaacaccg gaatgggcct
    26041 cgcaaatcac cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta
    26101 ccaaaccggc gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg
    26161 caacccgtgc tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aatggaggga
    26221 acagcggcgc gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accttggaaa
    26281 acccgatcca gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg
    26341 aaatgacggc gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa
    26401 tgatctggaa ctatgccggt aactgcctga ttaaccagca ttctgaaatc aaccgtaccc
    26461 atgaaatcct ccaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga
    26521 cctcatcggc gaaatatgct gacatcctgc tgcctgactg caccgcttcc gaacagatgg
    26581 actttgcact ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga
    26641 ttaaaccgcg ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc
    26701 ttggcgttga gcaacagttt actgaaggcc gtacccagga agagtggatg cggcatctgt
    26761 atgcccagtc gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgcaagcagg
    26821 ggatctttaa aaagcgcgac ccacaagggc atcacgttgc ttataaagcc ttccgtgaag
    26881 atccgcaggc aaacccactg actacgccat cgggcaaaat tgagatttat tcgcaggcgc
    26941 tggctgacat tgccgctacc tgggaattgc cagaaggcga tgtgatcgat ccactgccga
    27001 tctacacgcc gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc
    27061 ttacaggttt ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga
    27121 aagcggcttg ccgtcaggaa atgtggatca acccgcttga tgcccaaaaa cgcggtatcc
    27181 acaacggcga taaagtcagg atctttaacg atcgtggtga ggttcatatt gaggcgaaag
    27241 tgacgccacg aatgatgccg ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg
    27301 atgcaaaacg tgtcgataag ggcggttgta tcaacgtact gaccactcaa cgtccgtctc
    27361 ctctcgctaa ggggaatccg tcacatacaa accttgttca ggttgaaaag gtgtaaggag
    27421 taaccgatga caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa
    27481 acctgcgagc tggcttgtaa agactacaaa gatttgacgc cagaagtcag tttccgccgc
    27541 atttatgaat atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg
    27601 tttgcctact atctgtcgat ttcgtgcaac cactgcgaag atccggcttg tactaaagtc
    27661 tgcccgagcg gtgcgatgca taaacgcgaa gatggttttg ttgtggtcga tgaagatgta
    27721 tgcattggct gccgttactg tcatatggct tgcccgtatg gcgcaccgca atataacgaa
    27781 acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag
    27841 ccgatctgtg ttgaatcttg cccgctgcgg gcgctggatt tcggccctat cgacgagctg
    27901 cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa
    27961 ccgaatattg tgatcaaacc caatgccaat agccgcccga ccggggatac cactggctat
    28021 ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat
    28081 cttcacggtc ttcgggcaat gtgtggcggg tggttttatc gttctggctt tggcgctgct
    28141 caaaggcgac ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt ttggcttatg
    28201 ggtactgatg ggcattggct ttatcgcctc tatgctccat cttggttcac cgatgcgcgc
    28261 ctttaactcg ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc
    28321 gatctttttt gctgtaggcg gcatcggctg gttgctggca atgctgaaaa agctgtcacc
    28381 ggcattgcgt acgctatggc tggtggtgac gatggttctt ggcgtcgtct ttgtctggat
    28441 gatggtgcgt gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc
    28501 gatgggctgg gcgatgcgtc tgctgccagc catttctgta ctggcactgg tggtgagtgg
    28561 cgtgatgtca gtgatgcagg gcacagagct ggcaactatt catagttctg tgcagcaggc
    28621 cgcagcgctg gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt
    28681 tgccctgtgc ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact
    28741 atccgtctca ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg
    28801 tttgcatatg accgtgggga tggccgtcgc aagctaatca gtaacaaccg gggtttcggc
    28861 cccggttctc ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata
    28921 atcaggaata tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcacg
    28981 gtgcagctcg caagccacgc cgaaccatgt catcaattgc gcaccagctt gcgacatgcg
    29041 atcccacgcc gaatgccggg taatttcatt aaaggtccct gatgcatcag tcacgacaaa
    29101 gacatcaaac ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac
    29161 cacaccggca ataattaact gttttttacc tgtcgctttg actgctttta caaaatcttc
    29221 gttatcccag gcgttaatat tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc
    29281 tttcagttct agaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt
    29341 tggtaagttg aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc
    29401 gggttcgata tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc
    29461 agcatcattt ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc
    29521 agtggtacgt ctgaaatcgg tcagacgcga tgcattgctc tgaaagcata gacgggaaat
    29581 atgagtttgc tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag
    29641 atcaataaaa acaatcagat gagattaatc atctcttcag cattagcgtt atgaatgagt
    29701 aatataaatc gataataccg ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt
    29761 tttggcggtg gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca
    29821 cgtatacccg gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgatcg
    29881 cggtgttaaa tacactcgtg ccgctttggc tcgcccagga acacatgtcc acatggcagg
    29941 taggcgttgt cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt
    30001 atgtcattaa acgcattggc tttaaccgca gctattatct gtcctccttc atttttgctg
    30061 ctggctgtgc cggccttggc ctgatgattg gtttctggag ctggttggcg tggcgttttg
    30121 tcgcgggcgt cggctgtgcc atgatttggg tggttgttga gagcgcgctg atgtgcagtg
    30181 ggacgtcacg taaccgtggg cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa
    30241 cgtttttagg ccagttactg gtcagcaaag tctcaaccga gctgatgagc gtattgccgt
    30301 gggttacagg tttgacattg gcagggatct taccgctgtt gtttacgcgt gtcctgaatc
    30361 agcaggctga aaaccatgat tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc
    30421 ggcttggcgt gaatggctgc attatctcag gaatcgttct gggatctcta tatggcctga
    30481 tgc
//
LOCUS       DAJTRH010000040        30116 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTRH010000040 DAJTRH010000000
VERSION     DAJTRH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..636)
                     /gene="wcaD"
                     /locus_tag="OO339_001986"
     CDS             complement(<1..636)
                     /gene="wcaD"
                     /locus_tag="OO339_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5926631.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILS"
     gene            complement(611..1828)
                     /gene="wcaC"
                     /locus_tag="OO339_001987"
     CDS             complement(611..1828)
                     /gene="wcaC"
                     /locus_tag="OO339_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5926632.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1825..2313)
                     /gene="wcaB"
                     /locus_tag="OO339_001988"
     CDS             complement(1825..2313)
                     /gene="wcaB"
                     /locus_tag="OO339_001988"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5926633.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2316..3155)
                     /gene="wcaA"
                     /locus_tag="OO339_001989"
                     /pseudo
     CDS             complement(2316..3155)
                     /gene="wcaA"
                     /locus_tag="OO339_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3248..5410)
                     /gene="wzc"
                     /locus_tag="OO339_001990"
     CDS             complement(3248..5410)
                     /gene="wzc"
                     /locus_tag="OO339_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5926634.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5413..5856)
                     /gene="wzb"
                     /locus_tag="OO339_001991"
     CDS             complement(5413..5856)
                     /gene="wzb"
                     /locus_tag="OO339_001991"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5926635.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5862..7001)
                     /gene="wza"
                     /locus_tag="OO339_001992"
     CDS             complement(5862..7001)
                     /gene="wza"
                     /locus_tag="OO339_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5926636.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7310..7459
                     /locus_tag="OO339_001993"
     CDS             7310..7459
                     /locus_tag="OO339_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926637.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7660..9243
                     /locus_tag="OO339_001994"
     CDS             7660..9243
                     /locus_tag="OO339_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5926638.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9695..11548)
                     /gene="asmA"
                     /locus_tag="OO339_001995"
     CDS             complement(9695..11548)
                     /gene="asmA"
                     /locus_tag="OO339_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5926639.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11570..12151)
                     /gene="dcd"
                     /locus_tag="OO339_001996"
     CDS             complement(11570..12151)
                     /gene="dcd"
                     /locus_tag="OO339_001996"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5926640.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12243..12884)
                     /gene="udk"
                     /locus_tag="OO339_001997"
     CDS             complement(12243..12884)
                     /gene="udk"
                     /locus_tag="OO339_001997"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5926641.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13202..13444
                     /locus_tag="OO339_001998"
                     /pseudo
     CDS             13202..13444
                     /locus_tag="OO339_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13443..14384
                     /gene="mdtA"
                     /locus_tag="OO339_001999"
                     /pseudo
     CDS             13443..14384
                     /gene="mdtA"
                     /locus_tag="OO339_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14384..17506
                     /gene="mdtB"
                     /locus_tag="OO339_002000"
                     /pseudo
     CDS             14384..17506
                     /gene="mdtB"
                     /locus_tag="OO339_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17507..20572
                     /gene="mdtC"
                     /locus_tag="OO339_002001"
     CDS             17507..20572
                     /gene="mdtC"
                     /locus_tag="OO339_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5926642.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20573..21988
                     /locus_tag="OO339_002002"
     CDS             20573..21988
                     /locus_tag="OO339_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5926643.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            21985..23388
                     /gene="baeS"
                     /locus_tag="OO339_002003"
     CDS             21985..23388
                     /gene="baeS"
                     /locus_tag="OO339_002003"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5926644.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23385..24107
                     /gene="baeR"
                     /locus_tag="OO339_002004"
     CDS             23385..24107
                     /gene="baeR"
                     /locus_tag="OO339_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5926645.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24298..24630
                     /locus_tag="OO339_002005"
     CDS             24298..24630
                     /locus_tag="OO339_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5926646.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24777..26138
                     /gene="yegQ"
                     /locus_tag="OO339_002006"
     CDS             24777..26138
                     /gene="yegQ"
                     /locus_tag="OO339_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5926647.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26480..26797)
                     /locus_tag="OO339_002007"
     CDS             complement(26480..26797)
                     /locus_tag="OO339_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926648.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27203..28102
                     /gene="yegS"
                     /locus_tag="OO339_002008"
     CDS             27203..28102
                     /gene="yegS"
                     /locus_tag="OO339_002008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5926649.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28184..28957)
                     /locus_tag="OO339_002009"
     CDS             complement(28184..28957)
                     /locus_tag="OO339_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5926650.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29063..30103)
                     /gene="gatD"
                     /locus_tag="OO339_002010"
     CDS             complement(29063..30103)
                     /gene="gatD"
                     /locus_tag="OO339_002010"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5926651.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         6943 a         8004 c         7799 g         7370 t
ORIGIN      
        1 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
       61 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      121 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      181 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      241 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      301 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      361 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      421 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      481 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      541 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      601 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      661 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      721 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      781 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
      841 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
      901 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
      961 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1021 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1081 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1141 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1201 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1261 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1321 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1381 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1441 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1501 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1561 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1621 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1681 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1741 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1801 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     1861 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     1921 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     1981 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2041 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2101 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2161 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2221 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2281 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2341 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2401 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2461 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2521 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2581 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2641 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2701 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2761 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     2821 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     2881 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     2941 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3001 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3061 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3121 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3181 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3241 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3301 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3361 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3421 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3481 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3541 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3601 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3661 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3721 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3781 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     3841 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     3901 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     3961 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4021 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4081 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4141 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4201 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4261 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4321 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4381 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4441 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4501 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4561 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4621 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4681 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4741 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4801 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     4861 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     4921 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     4981 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5041 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5101 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5161 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5221 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5281 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5341 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5401 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5461 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5521 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5581 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5641 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5701 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5761 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     5821 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     5881 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     5941 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6001 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6061 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6121 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6181 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6241 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6301 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6361 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6421 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6481 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6541 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6601 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6661 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6721 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6781 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     6841 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     6901 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     6961 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7021 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7081 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7141 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7201 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7261 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7321 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7381 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7441 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7501 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7561 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7621 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7681 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7741 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7801 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     7861 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     7921 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     7981 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8041 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8101 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8161 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8221 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8281 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8341 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8401 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8461 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8521 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8581 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8641 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8701 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8761 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     8821 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     8881 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     8941 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9001 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9061 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9121 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9181 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9241 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9301 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9361 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9421 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9481 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9541 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9601 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9661 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9721 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9781 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
     9841 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
     9901 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
     9961 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10021 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10081 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10141 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10201 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10261 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10321 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10381 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10441 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10501 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10561 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10621 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10681 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10741 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10801 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    10861 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    10921 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    10981 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11041 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11101 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11161 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11221 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11281 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11341 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11401 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11461 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11521 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11581 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11641 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11701 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11761 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    11821 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    11881 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    11941 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12001 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12061 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12121 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12181 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12241 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12301 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12361 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12421 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12481 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12541 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12601 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12661 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12721 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12781 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    12841 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    12901 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    12961 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13021 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13081 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13141 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13201 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13261 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13321 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13381 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13441 cattccagga aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc
    13501 agttcaaagt tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg
    13561 ctaacgcccg ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc
    13621 gtcaggagct ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg
    13681 atgaagcaag cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag
    13741 tcgatggtcg cgttggtctc aagcaagttg atgttggtaa ccaaatctcc agtggtgata
    13801 ccaccggaat tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg
    13861 aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg atggtagaag
    13921 cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc
    13981 aaatcgatgc cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg
    14041 cgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg
    14101 tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga
    14161 atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag
    14221 tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc
    14281 tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga
    14341 aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc
    14401 gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct
    14461 gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct
    14521 gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga
    14581 tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct
    14641 gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct
    14701 aacattaccg ctcaatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa
    14761 cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc
    14821 gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat
    14881 ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct
    14941 ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct
    15001 cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa
    15061 aggtagcctc gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc
    15121 cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg
    15181 cgatgtcgca actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa
    15241 agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc
    15301 cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt
    15361 gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga
    15421 attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc
    15481 ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat
    15541 ggtgtttctc gatttttcaa tcaataacct gacactgatg gctttaacta tcgccaccgg
    15601 attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg
    15661 cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc
    15721 gctgaccttc tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg
    15781 gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt
    15841 gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa
    15901 acagaacagc ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg
    15961 tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac
    16021 gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga
    16081 caacggcatt attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc
    16141 ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac
    16201 ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa
    16261 cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac
    16321 ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat
    16381 cgatactcag gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc
    16441 gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac agctttctga
    16501 tgtctccagc gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag
    16561 cgccagccgt ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg
    16621 tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggagca
    16681 caataccgaa aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga
    16741 cggtggcgtg gtgccgttaa gctcaattgc caaaattgag cagcgttttg cgccgctttc
    16801 catcaaccat ctggatcagt tcccggtaac aaccatctcc tttaacgtgc cggataacta
    16861 ttcgctgggc gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt
    16921 ggatatcacc acgcaattct agggcagcac cctcgccttc cagtcggcgc tgggcagcac
    16981 tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga
    17041 gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct
    17101 gctggcatta atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt
    17161 gctgatcggt attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga
    17221 gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg
    17281 tccgatcctg atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac
    17341 cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt
    17401 cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc
    17461 attgtggacc aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc
    17521 cctcttcatt taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg
    17581 catactgggt ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat
    17641 tatggtcagc gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac
    17701 gccgctggag cgctcacttg ggcgcattgc cggggttagt gaaatgacct ccagcagttc
    17761 gctcggcagc acgcgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc
    17821 gcgtgatgtg caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag
    17881 ccgcccgacc tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac
    17941 atccgatact tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac
    18001 gatttcgcaa atcgacggtg tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt
    18061 gcgcgtcggg ctgaccccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg
    18121 cactgccatc agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca
    18181 ccgctggcag atccagacca acgatgaact gaaaaccgcc gctgaatatc agccgctgat
    18241 tattcactac aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgactgactc
    18301 agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat
    18361 ccgcaaactg ccggaagcca atattatcca gacggtagac agcatccggg caaaattacc
    18421 agagttgcag gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc
    18481 caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt
    18541 gattctggtg gtctttttat tcctgcgctc gggtcgcgcc actattattc ccgccgttgc
    18601 ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa
    18661 taatctttcg ctgatggcgc tcaccattgc tactggtttc gtggtggatg acgccattgt
    18721 ggtgctggaa aacattgccc gtcatctgga agcggggatg aaaccactac aggccgcgct
    18781 gcaaggtact cgcgaagtcg gctttacggt gctgtcgatg agtctgtcac tggtggcggt
    18841 gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt
    18901 gacgctttct gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat
    18961 gtgcggctgg atgctaaaag ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg
    19021 ccgcatgttg gtagccctgc aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca
    19081 tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct cgatcccgaa
    19141 aaccttcttc ccggagcagg acaccggcgt actgatgggt gggatacagg cggatcagag
    19201 tatttcgttt caggcgatgc gcggcaaatt gcaggatttc atgaaaatta tccgtgacga
    19261 tccggcagtg gataatgtca ccggctttac cggcggttca cgagttaaca gtgggatgat
    19321 gtttatcacc ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg
    19381 tctgcgcgtg aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga
    19441 tattcgcgtt ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga
    19501 cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga
    19561 actggcggac gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga
    19621 ccgcgacacc atggcacggc tgggaatcga cgtacaggcc gccaacagtc tgttaaataa
    19681 cgccttcggt cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt
    19741 gatggaagtg gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat
    19801 caataacgaa ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc
    19861 cccactatcg gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc
    19921 gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg
    19981 tgtgccttcg acggtgcgcg gcagttttgc cggcacggcg caggttttcc aggagacgat
    20041 gaactcgcag gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat
    20101 cctttacgag agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt
    20161 tggagcgctg ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg
    20221 gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct
    20281 tgaagcccaa cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct
    20341 gcgttttcgc ccgattatga tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt
    20401 attgtcgggc ggtgacggct cggagctgcg gcaacccctg gagatcacca ttgtcggcgg
    20461 tctggtgatg agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga
    20521 ccgtctgcgg ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga
    20581 tcttcccgac agcacccgct ggcaattatg gattgtggct ttcggcttct ttatgcagtc
    20641 gctggacacc accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag
    20701 tccgttgcat atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc
    20761 cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt
    20821 gctgtttact ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct
    20881 ggcacgcgcg ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt
    20941 gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg
    21001 tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg
    21061 gcactggatc tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat
    21121 gttaatgccg aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct
    21181 ggcggttggc atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc
    21241 gccgctggcg attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct
    21301 gcacgccaga aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt
    21361 ttcgctgggc ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat
    21421 gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat
    21481 gatcccgatg gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg
    21541 ctttggttat cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt
    21601 gtttatgact accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca
    21661 agggatggtc aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc
    21721 ggacaatctg gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag
    21781 tatcggcgtc accatcgccg ggctgttgct ggggcttttt ggttcgcagc atatcagcgt
    21841 cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat
    21901 cgcccttccg gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat
    21961 ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg
    22021 tttctggcga ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt
    22081 atcagttttg agcgtggctt tattgattac atcaaacacg gcaatgaaca gcgattacag
    22141 ctgttaagtg atgcgcttgg cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc
    22201 aacaacgatc gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat
    22261 aaacccggcc cgggtatgcc gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa
    22321 aacaacaaag tactggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc
    22381 attctggtca acggtgcgga agtgggcgcg gtgattgcct cccctgttga gcggctgacc
    22441 cgcaatactg atatcaattt cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc
    22501 ctggcaacgt tactcgcggc gctcgccacc tttctgctgg cgcgcggttt gctggcaccg
    22561 gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta
    22621 acgcccacca gtgaagatga actgggcaaa ctggcgcaag acttcaacca gctcgccagc
    22681 acgctggaga aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg
    22741 cgtacgccat tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa
    22801 ttcacgccgg agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt
    22861 gacgatcttc atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg
    22921 gtagatttga tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc
    22981 cgtggcctga aactgcaatt ttccctgcca gacagtatca ccgtatttgg cgatcgcgac
    23041 cgtttaatgc agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc
    23101 ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac
    23161 agtgcgccag gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc
    23221 gaaggttccc gcaaccgcgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac
    23281 attgttgaag cccataatgg ccgcattatt gccgcccatt cgccttttgg cggggtaagc
    23341 attacagtag agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa
    23401 tcgacgaaaa cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc
    23461 tcattgatta tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg
    23521 tactggcgta tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg
    23581 gcaccgatgg cctgacatta tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga
    23641 tggtgacggc aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg
    23701 actacatctg caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc
    23761 gccgttgtaa accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgattatcg
    23821 acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat
    23881 ttcgtctgct taaaacgctc tctcacgaac caggaaaagt gttctcccgc gagcaattgc
    23941 tcaatcatct ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta
    24001 aaaatctgcg ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt
    24061 acggcgttgg ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt
    24121 actttgttag ccggagatgg cgttcagacc cgatccggca acattattcc acgcatggtc
    24181 agcaaactga cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc
    24241 agtaaacagc acctttagat gctgttttcg ataagcaatt tcaatcaagg agtcattatg
    24301 gctggttggt ttgaactcag taagagcagt gataatcagt tccggtttgt gctaaaagcg
    24361 ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag
    24421 ggcatcgcgt cggtgcgtag caacagcccg caagaagagc gctatgagaa aaaaacggca
    24481 agcaacggca aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc
    24541 cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc
    24601 acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc
    24661 cggcgcttta ccagatcccc cctacagcgc tacaatgccc gcccttaaag tgggggcctc
    24721 ccctaaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt
    24781 ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt
    24841 atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg
    24901 aattcaacca cgaaaatctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt
    24961 tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc
    25021 tgaaaccggt ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta
    25081 tgctggtgcg tgagcacttc cctgaaatgc cgatccacct ctcggtgcag gctaacgccg
    25141 tgaactgggc gacggtgaaa ttctggcagc aaatgggact gacccgcgtg atcctctctc
    25201 gcgagctgtc gctggaagag attgaagaga tccataatca ggtgccggat atggagatcg
    25261 agatcttcgt tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct
    25321 atatcaacaa gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca
    25381 acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc
    25441 cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga
    25501 tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt
    25561 acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg
    25621 gcgtgcattc gctgaaaatc gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg
    25681 cacaggttta ccgcaaagct atcgatgacg ccgctgcggg aaaaccgttc gataccagcc
    25741 tgctggaaac tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc
    25801 atactcacga cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt
    25861 ttgttggtga gtttaccggt gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata
    25921 aattctccgt tggcgacagt cttgagctga tgacgccgca aggcaacatt aactttaccc
    25981 ttgagcacat ggaaaacgcc aaaggcgaag ctatgccggt cgcaccaggc gatggttata
    26041 ctgtgtggct cccggtcccg caggatcttg agctaaatta cgcgctgcta atgcgtaatt
    26101 tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg
    26161 gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt
    26221 tcgctgaaaa acataatcca taaaatgcta gctgtaccag gaaccacctc cttagcctgt
    26281 gtaatctccc ttacacgggc ttattttttg tacataagct gtaaagaagg attgatccct
    26341 gataaccgtc ttccattgac cacatcgata gaatccccct tcatagcaca atgcctttca
    26401 cgtaacggta tcgtgctcgc ccaggttccg gctacgcaca gccagaacgc gcatgtttga
    26461 cgcttacaaa aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta
    26521 atcctgcacc ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga
    26581 aatgggctgc atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag
    26641 aagcttcggc ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg
    26701 caagaccaaa tttaccgata cttgtttcat cattatgcac agcacaacca gatataagaa
    26761 aaatactaat taatgatata gcagctatct ttttcatctc accttccccc attaaatacc
    26821 aacgacactc tctagtgttt aaatataata atggcatgat tattataatt gaataggatt
    26881 ataataaatg ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct
    26941 tcagggtaac tccatggggg ctttaatata ttatattgaa gatgccactg tttagttgaa
    27001 tattaggtat atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc
    27061 cattgcaagc tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct
    27121 ccgactttca tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc
    27181 aatcataaag gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg
    27241 taaaagtact gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat
    27301 gacgatccat gtgcgggtca cctgggagaa aggtgatgcc gcacgatttg tagaggaggc
    27361 ccggaagttg ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt
    27421 ttctacggcg ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt
    27481 aggaaccgcc aatgattttg ccaccagtgt agggattcct gaggcactgg ataaggcgct
    27541 gaaactggca attgccggta atgccattgc gatagatatg gcgcaggtca acaaacaaac
    27601 ctgttttatt aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga
    27661 aaaattaaaa gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga
    27721 tactctgcaa ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc
    27781 cctggtcatt ggtattggta acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa
    27841 cgcgttaatt aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc
    27901 ggctctcgta tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc
    27961 gtcgtggttt gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt
    28021 gagtgggcaa aacttccata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc
    28081 agattgtcca ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgtaat
    28141 gccggatgcg gtgtaaacac cttatccggc atacagaaca atactacacc gtaattaatt
    28201 ttacccgtgt cttctgcaat gccaatttat ccacctctga cacagcatca tcggtaacaa
    28261 taatgtcaat tttttcgatt ggcagtacct gattaaaacc gcgacgatta aatttcgatg
    28321 aatcgagaac ggcaatcact ttatttgcgg cggtgaccat cgccccgcta atcgaataac
    28381 cttcattaaa ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta
    28441 aatcggcatt aatatcctgc aatgaacgct cggcaatgga accatgcatc gagcgcgttt
    28501 tatggcggac cgtgccacca cagacgacca gagtaatgtc tttattttcg gaaagggcaa
    28561 acgctgccgg gagactgttg gtaatcaccg tgatattttt agcggtcatt aatccttcgg
    28621 cgataagcat tgtggtactt ccgctgtcga gaataacggt catcccttca tggatcattt
    28681 tgactgccgc ctgcgctatt ctgttttttg gcgcgctggc gagctgaaat cgctctttaa
    28741 acccgacttc ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat
    28801 gaaagcgcgt aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt
    28861 ccgaggcagc aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta
    28921 attggatgat cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg
    28981 tggctagtgt aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc
    29041 agcatcacgc tggcccgcgg tttcatggaa tgagcaacac tttgccctgc atggcattac
    29101 gagcgatgtc acgcaccgcc tgggtgaagc tttcaaagct tccacggtga gcgattaatg
    29161 gctccaggct taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg
    29221 gccaagggct ggaatagttc atccaactgc cgataaccgt cagctcttta cgcaatattt
    29281 tgccaaacgt tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt
    29341 gggcatgagg accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct
    29401 cgaggataag ctgattaaag cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca
    29461 tttcgctact gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg
    29521 agctaatgtc gatcgccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca
    29581 gcaggccaat ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta
    29641 aatgaaaagc atgcaggcca acggtaatcg gctcaataaa agccccatcc tcaataggca
    29701 tatccgtggg tagagcaaag acattttttc gcttaacgac aatatattca gcaaatccac
    29761 catcacgccg cgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcaaac
    29821 actctggaca agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccagggtgta
    29881 aatcatcaac accggatccc accgcatcaa tatagccgct aaattcatgg cctaacgtta
    29941 ttggataata atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg
    30001 agctggcaat ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttttg
    30061 caacgcgcac gataccatca gtatcattca ctactgattt cataaaaact cctgat
//
LOCUS       DAJTRH010000041        30013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTRH010000041 DAJTRH010000000
VERSION     DAJTRH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO339_002011"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO339_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5926652.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO339_002012"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO339_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5926653.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO339_002013"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO339_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5926654.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO339_002014"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO339_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5926655.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO339_002015"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO339_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5926656.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO339_002016"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO339_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5926657.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO339_002017"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO339_002017"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5926658.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO339_002018"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO339_002018"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5926659.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO339_002019"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO339_002019"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5926660.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO339_002020"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO339_002020"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5926661.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO339_002021"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO339_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5926662.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO339_002022"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO339_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5926663.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO339_002023"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO339_002023"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5926664.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO339_002024"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO339_002024"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5926665.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO339_002025"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO339_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5926666.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO339_002026"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO339_002026"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5926667.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO339_002027"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO339_002027"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5926668.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO339_002028"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO339_002028"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5926669.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO339_002029"
     CDS             21622..22212
                     /locus_tag="OO339_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5926670.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO339_002030"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO339_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5926671.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO339_002031"
     CDS             22684..23379
                     /locus_tag="OO339_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5926672.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO339_002032"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO339_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5926673.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO339_002033"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO339_002033"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5926674.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26743
                     /gene="zraR"
                     /locus_tag="OO339_002034"
                     /pseudo
     CDS             25417..26743
                     /gene="zraR"
                     /locus_tag="OO339_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(26740..28029)
                     /gene="purD"
                     /locus_tag="OO339_002035"
     CDS             complement(26740..28029)
                     /gene="purD"
                     /locus_tag="OO339_002035"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5926675.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28041..29630)
                     /gene="purH"
                     /locus_tag="OO339_002036"
     CDS             complement(28041..29630)
                     /gene="purH"
                     /locus_tag="OO339_002036"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5926676.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7029 a         7823 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cagccacgct
    25621 gaaagagatc aaagcgttaa acccggcaat tccggtgctg attatgactg cgtactccag
    25681 cgtcgagacg gcggtagagg cactgaaaac tggggcgcag gattatctca tcaagccgct
    25741 ggatttcgat aacctacagg cgacgctgga aaaggcgctc gcgcatacgc acagtattga
    25801 tgctgaaaca cctgcggtga ctgccagcca gttcggtatg gtcggtaaaa gcccggcgat
    25861 gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg gaagccacgg tgctgatcca
    25921 cggcgattcc ggcaccggta aagagctggt cgccagggcg attcacgcca gtagcgcacg
    25981 tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc aacgaatcct tgctggaatc
    26041 tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc gataaacgcc gggaagggcg
    26101 ctttgttgag gcggacggcg gcacgctgtt tctcgatgaa attggcgata tctcgccgat
    26161 gatgcaggtg cgtctgctgc gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa
    26221 ccagactatc tcggttgatg tccggctgat tgcggcgacc catcgcgatc ttgccgcaga
    26281 ggtgaatgcc gggcgttttc gccaggatct ctactatcgc ctgaatgtgg tggcgattga
    26341 agtaccatcg ctgcggcaac ggcgggaaga tatccctctg ctggctggcc attttcttca
    26401 gcgctttgcc gagcgtaatc gcaaggcggt aaaaggcttt acgccacagg cgatggattt
    26461 gctgatccac tacgactggc cgggaaatat tcgtgaactg gaaaacgcgg tggaacgggc
    26521 ggtggtgctg ctgaccgggg aatatatttc cgaacgcgag ctgccgctgg cgattgccag
    26581 tacgccgatc ccgctggggc aaagtcagga tattcagccg ctggtggaag tggaaaaaga
    26641 ggtgattctg gcggcgctgg agaaaacggg cggcaacaaa accgaagccg cccgtcagtt
    26701 agggatcacg cgcaaaacgc tattggcaaa actgtcgcgt tagttctgct cgcgttcgat
    26761 ggcgcgccaa ccgatatctt tccggcagaa gcaatcatcc cagtggatat cggtcattaa
    26821 ggcgtaggcg cgtttctgcg cttctgccac ggtatgaccc agcgcggtga cgcacagtac
    26881 gcgcccgccg ctggttacca cctgctcgtc atccgccagt tttgtgcccg cgtggaacac
    26941 tttgccgtca gccacttctt ccagcggcag gccgtggatc acatcgccgg tgcggtaatc
    27001 gcccggatat ccacccgcag ccatcaccac gctgagagaa gcacgttcat cccactctga
    27061 cgttttctcg tccagtttgc cttcacaggc cgccaggcag agctcaacca gatcggactt
    27121 catgcgcagc ataatcggct gggtttccgg atcgccaaag cggcagttaa attcaataac
    27181 cttcggattg ccctgtttgt cgatcatcag gcccgcatag agaaaaccgg tgtaggtgtt
    27241 gccttccgcc gccatgcctt tcacggttgg ccagatgata cgttccatgg tgcgctgatg
    27301 aacttcatcg gttactaccg gcgcggggga gtaagcgccc atcccgccgg tgtttggtcc
    27361 gttatcttta tcgcctacgc gtttgtgatc ctggctggta gccatcggca gcacatgctc
    27421 gccgtccacc atcacgataa agctcgcttc ttcgccatcg aggaactctt cgataacgat
    27481 gcgatgaccc gcgtcgccaa aagcgttgcc cgccagcata tcgtgaacag ccgcttccgc
    27541 ttcttccagc gtcatcgcca cgataacgcc tttcccggca gccagaccgt ccgctttaat
    27601 gacgattggc gcgcctttct cacgcagata caccagcgca ggttctacct cggtgaagtt
    27661 ctggtattcc gccgtaggga tcttatggcg ggccaggaaa tctttggtaa acgcttttga
    27721 gccttccagt tgggccgcac ctgcggttgg gccgaagatt ttcagcccgg cggcgcggaa
    27781 ggtatcgacc acgcctttca ccagcggcgc ttccgggccg acgatggtca gatcaatctt
    27841 ttcgttttgt gcgaaatcca acagcgccgg gatatcggtc acgccgatag caacgttttg
    27901 cagcgcgggt tccagcgcag tgcctgcatt acccggagca gcaaaaacag tctcaaccag
    27961 cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc ccgccgttac caatcactaa
    28021 tactttcatc tattgctcca ttaatggcgg aagtggcgca tgtcggtgaa gagcatcgca
    28081 ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac ggatggaacc gccaggctgg
    28141 attacgcagg tcacgcccgc agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac
    28201 gcgtcagaag ccatcgagga acctttcact tccaggcctt catcggcagc tttaataccg
    28261 gcgattttcg cggagtacac gcggctcatc tggcccgcgc caataccgat ggtcatattg
    28321 tttttggcat agacgatagc attggatttc acgaatttcg ccactttcca gcagaacagc
    28381 gcatcacgca gttcctgttc ggtcggctga cgttgggtga cgacgcgcag ttcttccgca
    28441 ccgaccatcc ccaggtcacg atcctgaacc agcagaccgc cgttcacgcg tttgaaatca
    28501 agacccggaa cacgctcgcc ccactgaccg caggtcagaa cgcgtacgtt ctgcttggcg
    28561 gcggtgattt tcagggcttc ttcgctggcg gaaggcgcaa taatcacttc gacaaactga
    28621 cgagaaatga tggcctgtgc ggtttccgca tccagctcgc ggttaaaggc aatgatgccg
    28681 ccgaatgcgg aggttgggtc ggttttgtac gcgcgatcgt aagcatcaag aatggaattg
    28741 ccgatagcta caccgcaagg gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac
    28801 tctttcacgc actccagcgc cgcatcggta tcggcgatgt tgttataaga gagggcttta
    28861 ccctgaacct gggttgcggt agcaacggag gcttctttca cattctcttc tatatagaag
    28921 gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg
    28981 ttcagcgtgc gtgggaagcg accggcggct tctttgcttt caccgtggta cgccggaacc
    29041 atgctgccga agtagttggc aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg
    29101 atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt cgttgtcatc catctcttta
    29161 ataatggcgt cgtagtcgct gctcttcacc acgattgcga catctttatg gttcttagcg
    29221 gcggagcgca ccatggtcgg gccgccgata tcgatgttct caaccgcatc ttccagcgag
    29281 cagccttcac gagcaacagt ctgggcgaac ggatacaggt taacaaccac catatcgata
    29341 ggctggatct gatgttcttc cataatggca tcgtcctggc cgcgacggcc cagaatgcca
    29401 ccatgtactt tcggatgcag ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg
    29461 taatcggaaa cttcggttac cggcagacct ttttctgcta acagacgggc agtgccccct
    29521 gtagacagca gctccacacc gcgtgcggaa agtgcctggg cgaattcgac gataccggct
    29581 ttgtcagaaa cactgagcag agcgcggcgg actggacgac gttgttgcat ggtaaatccc
    29641 ctgggtttga ctattacaga gagcgttagc tgaatttttc gcgaaaaact cagctaacgc
    29701 ccctaacggg gcatccttat ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc
    29761 gcgaattttt ctctttcaat ggtgatcaca attttgacag tggttaccgt gggaaaaata
    29821 cagaaattac attgatgatt gtggataact ctgtgcgtaa aaaggtataa agcggggttt
    29881 tgctggggaa tgcagcagtc agtcattttt ctgcaatttt tctattgcgg cctgcggaga
    29941 actccctata atgcgccttc atcgacacgg cggatgtgaa tcacttcaca caaacagccg
    30001 gttcggttga aga
//
LOCUS       DAJTRH010000042        29643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTRH010000042 DAJTRH010000000
VERSION     DAJTRH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(1..441)
                     /locus_tag="OO339_002037"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO339_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO339_002038"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO339_002038"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5926677.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO339_002039"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO339_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5926678.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO339_002040"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO339_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5926679.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO339_002041"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO339_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO339_002042"
     CDS             5754..6257
                     /locus_tag="OO339_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5926680.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO339_002043"
     CDS             complement(6337..6588)
                     /locus_tag="OO339_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5926681.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO339_002044"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO339_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5926682.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO339_002045"
     CDS             7052..7375
                     /locus_tag="OO339_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5926683.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO339_002046"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO339_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5926684.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO339_002047"
     CDS             complement(8080..8319)
                     /locus_tag="OO339_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5926685.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO339_002048"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO339_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5926686.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO339_002049"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO339_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5926687.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO339_002050"
     tRNA            complement(10643..10729)
                     /locus_tag="OO339_002050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO339_002051"
     tRNA            complement(10742..10815)
                     /locus_tag="OO339_002051"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO339_002052"
     tRNA            complement(10870..10945)
                     /locus_tag="OO339_002052"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO339_002053"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO339_002053"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5926688.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO339_002054"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO339_002054"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5926689.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO339_002055"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO339_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5926690.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="OO339_002056"
     CDS             14483..14659
                     /locus_tag="OO339_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926691.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="OO339_002057"
     CDS             14646..14870
                     /locus_tag="OO339_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5926692.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO339_002058"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO339_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO339_002059"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO339_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5926693.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO339_002060"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO339_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5926694.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO339_002061"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO339_002061"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5926695.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO339_002062"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO339_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5926696.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO339_002063"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO339_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5926697.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO339_002064"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO339_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO339_002065"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO339_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5926698.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="OO339_002066"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="OO339_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5926699.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="OO339_002067"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="OO339_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5926700.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="OO339_002068"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="OO339_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5926701.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="OO339_002069"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="OO339_002069"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5926702.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="OO339_002070"
     CDS             complement(26368..26781)
                     /locus_tag="OO339_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5926703.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="OO339_002071"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="OO339_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5926704.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="OO339_002072"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="OO339_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5926705.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="OO339_002073"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="OO339_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..>29643)
                     /locus_tag="OO339_002074"
     CDS             complement(29220..>29643)
                     /locus_tag="OO339_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5926706.1"
                     /translation="MIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYE
                     MRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADV
                     NGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7372 a         7664 c         7053 g         7554 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtagt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca
    29461 agcacctgac cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag
    29521 ccctgatcca gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg
    29581 ctgaccgtac ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc
    29641 att
//
LOCUS       DAJTRH010000043        29519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTRH010000043 DAJTRH010000000
VERSION     DAJTRH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            57..851
                     /locus_tag="OO339_002075"
     CDS             57..851
                     /locus_tag="OO339_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5926707.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            873..2282
                     /locus_tag="OO339_002076"
     CDS             873..2282
                     /locus_tag="OO339_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5926708.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="OO339_002077"
     CDS             2260..3039
                     /locus_tag="OO339_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5926709.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="OO339_002078"
     CDS             3036..3896
                     /locus_tag="OO339_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5926710.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="OO339_002079"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="OO339_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="OO339_002080"
     CDS             complement(4636..4896)
                     /locus_tag="OO339_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5926711.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="OO339_002081"
     CDS             complement(4887..6158)
                     /locus_tag="OO339_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5926712.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="OO339_002082"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="OO339_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5926713.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="OO339_002083"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="OO339_002083"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5926714.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="OO339_002084"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="OO339_002084"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5926715.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="OO339_002085"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="OO339_002085"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5926716.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="OO339_002086"
     CDS             11500..11652
                     /locus_tag="OO339_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5926717.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11846..12937
                     /locus_tag="OO339_002087"
                     /pseudo
     CDS             11846..12937
                     /locus_tag="OO339_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13083..14120)
                     /gene="iap"
                     /locus_tag="OO339_002088"
     CDS             complement(13083..14120)
                     /gene="iap"
                     /locus_tag="OO339_002088"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5926718.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14372..15280
                     /gene="cysD"
                     /locus_tag="OO339_002089"
     CDS             14372..15280
                     /gene="cysD"
                     /locus_tag="OO339_002089"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5926719.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15282..16709
                     /gene="cysN"
                     /locus_tag="OO339_002090"
     CDS             15282..16709
                     /gene="cysN"
                     /locus_tag="OO339_002090"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5926720.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16709..17314
                     /gene="cysC"
                     /locus_tag="OO339_002091"
     CDS             16709..17314
                     /gene="cysC"
                     /locus_tag="OO339_002091"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5926721.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17364..17687
                     /locus_tag="OO339_002092"
     CDS             17364..17687
                     /locus_tag="OO339_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5926722.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17881..18192
                     /gene="ftsB"
                     /locus_tag="OO339_002093"
     CDS             17881..18192
                     /gene="ftsB"
                     /locus_tag="OO339_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5926723.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18211..18921
                     /gene="ispD"
                     /locus_tag="OO339_002094"
     CDS             18211..18921
                     /gene="ispD"
                     /locus_tag="OO339_002094"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5926724.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18921..19400
                     /gene="ispF"
                     /locus_tag="OO339_002095"
     CDS             18921..19400
                     /gene="ispF"
                     /locus_tag="OO339_002095"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5926725.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19397..20446
                     /gene="truD"
                     /locus_tag="OO339_002096"
     CDS             19397..20446
                     /gene="truD"
                     /locus_tag="OO339_002096"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5926726.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20427..21188
                     /gene="surE"
                     /locus_tag="OO339_002097"
     CDS             20427..21188
                     /gene="surE"
                     /locus_tag="OO339_002097"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5926727.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21182..21808
                     /gene="pcm"
                     /locus_tag="OO339_002098"
     CDS             21182..21808
                     /gene="pcm"
                     /locus_tag="OO339_002098"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5926728.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21948..23087
                     /gene="nlpD"
                     /locus_tag="OO339_002099"
     CDS             21948..23087
                     /gene="nlpD"
                     /locus_tag="OO339_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5926729.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23150..24142
                     /gene="rpoS"
                     /locus_tag="OO339_002100"
     CDS             23150..24142
                     /gene="rpoS"
                     /locus_tag="OO339_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5926730.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24262..24669)
                     /locus_tag="OO339_002101"
     CDS             complement(24262..24669)
                     /locus_tag="OO339_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5926731.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24816..25409
                     /locus_tag="OO339_002102"
     CDS             24816..25409
                     /locus_tag="OO339_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5926732.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25409..26356
                     /locus_tag="OO339_002103"
                     /pseudo
     CDS             25409..26356
                     /locus_tag="OO339_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26629..27192)
                     /locus_tag="OO339_002104"
     CDS             complement(26629..27192)
                     /locus_tag="OO339_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926733.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27357..27608
                     /locus_tag="OO339_002105"
     CDS             27357..27608
                     /locus_tag="OO339_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5926734.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27610..29519
                     /locus_tag="OO339_002106"
                     /pseudo
     CDS             27610..29519
                     /locus_tag="OO339_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7200 a         7335 c         7987 g         6997 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcattaattt attcataatg taaatgtttt attggctgat
    11821 atatatcata gatagttttt aaaatatgcg taaatatcct ttaagtttac tgaaggataa
    11881 aaatattgtg actttctttg atttctgggg aaaaaaccga cgtggcgaga aagagggtat
    11941 cgacgactat caccttcttt gctggcattt gctggatgtg gccgcaatgg gctatttaat
    12001 ggttaaaaga aattgcttcg ggctggctga ttattttcgt caattaggga tttctgacaa
    12061 ggaacaggca gctcgatttt tcgcctggtt gctgtgctgg cacgatattg gaaaatttgc
    12121 ccgctctttt caacaacttt acctgccccc tgaattcaag attcaggaag gtgccagaaa
    12181 aaattacgaa aaaatctctc attcaacgct gggctactgg ttgtggaatc attatttaag
    12241 tgaatgtcag gagttacttc cttcatcttc gctctctccg cgcaaactta ggcgagtgat
    12301 agaaatgtgg atgcccgtga cgacagggca tcatggtcgg ccccctgatc gtatggatga
    12361 attggataat tttctgcctg aagacaaagc tgcagcacga gattttctcc ttgaaatcaa
    12421 gccactgttt ccgcgaatag agatccccac attctgggat gatgacgagg gcgttgaact
    12481 tataaaacaa ctttcctggt atatctctgc aaccgtcgta cttgcagact ggacagggtc
    12541 gtcaacgcga ttttttccac gcgtcgcaca ggcaatggat attgaagatt actggcaaaa
    12601 ggctttagtt caggctcaaa acgcattaac cgtctttccg ccacaagcag aaaccgcgcc
    12661 tttcaccgga attaatacgc tgtttccttt tattgagaat acgacaccgt tacagcaaaa
    12721 ggtgctggat ctggatatca atcagccagg accacagttg tttattctgg aagatgtgac
    12781 cggcgcaggt aaaacagaag cggcgcttat cctggcgcac aggttgatgg ctgcgggtaa
    12841 cgctcagggg ctgttttttg gcctgccaac aatggcaacg gccaatgcca tgtacgatcg
    12901 actggtcaaa acctggcttg ctttctattc gccagaggtg ttccccgctc cagcggagat
    12961 aaaccggcgc aatacggtgc ggttttcccg gaagtaaacc ggacgcactg gatgcgatga
    13021 tgggtatcac ttagaattcc ccgcccctgc ggtagaactc ccagctccca ttttcaaacc
    13081 catcaagacg ccttcgccag ctccttcacc aacggtagca ttatccgcat aacgtcacgg
    13141 cagcgacgtt ctattcttcc aggaagtgcc ttatcaatat gttgttgatt atccagtctt
    13201 acgtcatgcc agctatttcc cgccgggaag gcaggtgttt ttgcgcgttg ctgataacca
    13261 tccttatttc caagattcca gttagtcgct tccaccgaaa gtacagcaat gcccgctttg
    13321 tcgaatattt ctgcgtcatt acaaccccca gtgcctttcg gataattttt attcaaaccc
    13381 ggattggtcg ttgcggctat tccgtgactg cgtgcaattg ccagcgccct gtcgcgcgtt
    13441 aatttcctta ctgcttcagg ggtttttaca ccgctgttga aatacaattt atcgccaaca
    13501 attaagttat cgagattaat caccagcagc gtatttttct tttcggtgtc actcatccgc
    13561 ttgagtaaat tctcagcgcc taattttcct tcctcttcgc cgctggtcgc cacaaatcga
    13621 ataccatact cggtaggcgt atttttcagg cgttctgcca gttccagcat gacacctaaa
    13681 cctgcggcgt tatcatctat tccttgtaac gtcagtccgc cgagattggc atctgcgtca
    13741 gcatcgctca gcggggcgta agtatccaga tgcgccataa tgatgatctg ctgcggcgct
    13801 ttgccttcat gagcggcaat caccgtactt cctgtcacgt tatgccagtt cttgcgatta
    13861 tcgcgggcgg tataaatata ccgactatta aatgtccgaa tatcactgcg ataacccatt
    13921 tgctgaaact gttggcgaat ataatcggca gataacattt ctgctggagt tccggtcatg
    13981 cgtcccggaa agaaagtagc aatatgtcgt gcctgggtat tagcaaaatc gccaggttta
    14041 ggtgacgagg cgtgtacggg gagaataaag catacgccga gcgccagggc agcggtacgg
    14101 tggcgcaatg cggaaaacat agtgagtcct taaataccat gcaaattttt ttaccgccat
    14161 agtatgaaac tgccgctgcg ctaaaacaat ttcaaatctt cctaaacgcc cgaaatccgg
    14221 tgccttaagc actttttgat attagctttg ccaaatcgtt attccgttaa ggaactactc
    14281 attctaattg gtaatttcat tcgttctctt acgctcccta tagtcgaaac atctgatggc
    14341 aagaaaatag cggtattgca aaggaacggt tatggctcaa aaacgactta ctcacctgcg
    14401 gcaactggag gcggaaagca tccacattat tcgcgaggtg gcggcagaat tctcaaatcc
    14461 ggtgatgctc tactctatcg gtaaagattc cagcgtcatg ctgcatctgg cgcgcaaggc
    14521 gttttatcca ggtacgctgc ctttcccgtt gctgcatgtc gataccggct ggaaattccg
    14581 cgagatgtat gagttccgcg atcgtacggc gaaagcctac ggctgcgagc tgctggtgca
    14641 taaaaacccg gaaggcgtgg cgatggggat taatccattc gtgcacggca gcgcgaaaca
    14701 taccgatatt atgaaaactg aaggcctgaa acaggcgctg aacaaatacg gttttgatgc
    14761 cgccttcggt ggtgcgcgcc gtgacgaaga gaaatcccgc gctaaagagc gaatctactc
    14821 tttccgtgac cgcttccatc gctgggatcc gaaaaatcag cgcccggagc tgtggcacaa
    14881 ctacaacggg caaattaaca aaggcgaaag catccgcgtc ttcccgctct ctaactggac
    14941 cgagcaggat atctggcaat acatctggct ggaaaatatc gacattgtgc cgctgtatct
    15001 cgctgcggaa cgtccggttc tggaacgcga cggtatgttg atgatgattg atgacaaccg
    15061 tatcgacctg caaccgggcg aagtgatcaa aaaacggatg gtgcgtttcc gtacgctggg
    15121 ctgctggccg ctgaccggtg cggtggagtc aaatgcacaa acactgccgg aaatcatcga
    15181 agagatgctg gtttccacca ccagtgaacg tcagggtcgc gtgattgacc gcgaccaggc
    15241 gggatccatg gagctgaaaa aacgtcaggg gtatttttaa gatgaacacc gtacttgcac
    15301 aacaaatcgc caatgaaggc ggcgtcgaag cctggatgat tgcgcaacaa cataaaagcc
    15361 tgctgcgttt tctgacctgt ggtagcgtcg atgacggcaa aagtaccctg attggtcgtc
    15421 tgctgcacga tacccgccaa atctacgaag atcagctctc atcgctgcat aacgacagta
    15481 agcgtcacgg aacccagggc gaaaagctgg atctggctct gctggtggac ggcctgcaag
    15541 ctgagcgcga acagggcatc actattgatg tggcctaccg ctatttctct accgagaagc
    15601 gtaaatttat tatcgccgac accccagggc acgagcagta cactcgcaat atggcgactg
    15661 gcgcatcgac atgtgaactg gcgatcttac tgattgatgc ccgtaaaggc gtgctcgatc
    15721 aaacccgtcg tcacagtttt atctccacac tgttggggat caaacatctg gtcgtggcga
    15781 tcaacaaaat ggatctggtg gattacagtg aagagacgtt cacccgtatt cgtgaagatt
    15841 atctgacctt tgccgggcag ctgccgggta atctggatat ccgctttgtg ccgctctccg
    15901 cactggaagg tgacaacgtg gcttcgcaaa gtgaaagtat gccgtggtac agcggtctga
    15961 cactgctcga agtgctggaa accgtggaga tccagcgagt ggtggatgcc cagccaatgc
    16021 gcttcccggt gcagtacgtt aaccgcccaa atctcgattt tcgtggctac gccggaatgc
    16081 tggcatccgg tcgcgtggaa gtcgggcaac gtgtaaaagt gctgccctct ggtgtggaat
    16141 caaacgtcgc gcggatcgtg acttttgatg gtgatcgcga agaagtcttt gccggagaag
    16201 cgatcaccct ggtgctgacg gatgagatcg atatcagccg tggtgatctg ctgctggcgg
    16261 cagacgaagc gttaccggcg gtgcagagtg cgtcggtgga tgtggtatgg atggcggaac
    16321 agccgctttc cccgggccag agttatgaca tcaaaattgc cggtaagaag acgcgtgctc
    16381 gtgttgatgg cattcgttat caggttgata ttaataacct tacccaacgc gaagttgaaa
    16441 acctgccgct gaacggcatc ggcctggtgg atctcacttt tgacgagcca ctggtgttag
    16501 atcgctatca gcaaaatccg gttaccggtg ggctgatttt tatcgatcgc ctgagcaatg
    16561 tgaccgtggg tgccggtatg gtgcacgagc cagttagcca ggcaacagct gcgtcatctg
    16621 aattcagtgc attcgaactg gaattgaatg ccctggttcg tcgccacttt ccgcactggg
    16681 gcgcgcgcga tttgctgggg gataaataat ggcgctgcat gacgaaaacg tcgtctggca
    16741 tagtcatccg gtcactgtgc aacaacgcga actacaccac ggtcatcgtg gtgtagtgct
    16801 gtggtttacc ggcctctccg ggtccggtaa atcaacggtc gccggggcgc tggaggaggc
    16861 gttacataaa ctcagcgtca gtacgtatct gctggatggc gacaatgttc gccacggatt
    16921 atgcagcgat ctcggtttta gcgatgccga tcgtaaagag aatatccgtc gcgtcggtga
    16981 agtggcgaat ttgatggttg aagccggact ggtggtgctg accgcattta tctcgccaca
    17041 ccgcgccgaa cgccagatgg ttcgcgaacg cgtaggagaa gggcgcttta ttgaagtgtt
    17101 tgtcgatacg ccgctggcga tttgcgaagc ccgcgatcca aaaggcttat ataagaaagc
    17161 gcgtgccggt gaactgcgca actttacggg aatagattcc gtttacgaag cgcctgaatc
    17221 ggcagaaatt catctcaatg gtgaacaatt agtaacaaat ttggtacagc aattattaga
    17281 tctgttgaga cagaacgata ttatcagatc ctgagacacc accgggtttc catgcccggt
    17341 tcgtcaaagt aacaggatca aatatgcgta atagccataa cattacacta acaaataacg
    17401 acagccttac tgaggatgaa gaaactacat ggtcactgcc tggtgccgtg gtcggtttta
    17461 tctcctggtt atttgcgctg gcgatgccga tgttgattta tggctctaac acgctgttct
    17521 tctttctcta cacctggcct ttctttctgg cactgatgcc tgtcgcggta gtggtgggga
    17581 ttgcgctgca ttcattgatg gacggaaagc tacgctacag tattgttttc accctggtga
    17641 cagtaggcat tatgtttggc gcactgttta tgtggctact gggctaattt gtactttctt
    17701 gtctcctctg ttcataattc aaaccgtaac taataatgag attatgttct gcacgccctg
    17761 ggtatacgta acaatgggca aatgtggtac atttgcccgc gttgtcgcgg tatccccaac
    17821 agaggatgta gagtcgtctt cggatgcatg ggatgatgat gcagtttttc agggggcagg
    17881 atgggtaaac taacgctgct gttgctggct attctggtct ggctacagta ttcgctgtgg
    17941 ttcggtaaga acggtataca tgactatacc cgcgtcaatg atgatgtggc ggcactgcaa
    18001 gctacaaacg cgaaacttaa agcgcgaaac gatcaacttt ttgccgaaat tgacgatctc
    18061 aatggcggcc aggaggcgct cgaagagcgt gcgcgtaatg aactcagcat gaccaggccg
    18121 ggcgaaactt tttatcgtct ggtgcctgac gcgtcgaagc gcgcacagtc tgcggggcaa
    18181 aacaatcgat aaatcagccc gggaattaac atggcaacca ctcatttgga tgtttgcgcc
    18241 gtggttccgg cggccggatt tggccgtcga atgcaaacgg aatgtcctaa gcaatatctc
    18301 tcaatcggta atcaaaccat tcttgaacac tcggtgcatg cgctgctggc gcatccccgg
    18361 gtgacacgtg tcgtcattgc cataagtcct ggcgatagcc gttttgcaca acttcctctg
    18421 gcgaatcatc cgcaaatcac cgttgtagat ggcggtgatg agcgtgccga ttccgtgctg
    18481 gcaggtctga aagccgctgg cgatgtgcag tgggtattgg tgcatgacgc cgctcgtcct
    18541 tgtttgcatc aggatgacct tgcgcgattg ttggcgttga gcgaaaccag ccgcacggga
    18601 gggatcctcg ccgcaccagt gcgcgatact atgaaacgtg ccgaaccggg caaaaatgcc
    18661 attgctcata ccgttgatcg caacggctta tggcacgcgc tgacgccgca atttttccct
    18721 cgtgagctgt tacatgactg tctgacgcgc gctctaaatg aaggcgctac cattaccgac
    18781 gaagcctcgg cgctggaata ttgcggattt catccgcagt tggtcgaagg ccgcgcggat
    18841 aacattaaag tcacgcgccc ggaagatttg gcactggccg agttttacct cacccgaacc
    18901 atccatcagg agaatacata atgcgaattg gacacggttt tgacgtacac gcctttggcg
    18961 gtgaaggccc aattatcatt ggtggcgtac gcattcctta cgaaaaagga ttgctggcgc
    19021 attctgatgg cgacgtggcg cttcatgcgt tgaccgatgc attgcttggc gcggcggcgc
    19081 tgggggatat cggcaaactg ttcccggata ccgatccggc atttaaaggt gccgacagcc
    19141 gcgagctgct acgcgaagcc tggcgtcgta ttcaggcgaa gggttatacc cttggcaacg
    19201 tcgatgtcac tatcatcgct caggcaccga agatgttgcc gcacattcca caaatgcgcg
    19261 tgtttattgc cgaagatctc ggctgccata tggatgatgt taacgtgaaa gccactacta
    19321 cggaaaaact tggatttacc ggacgtgggg aagggattgc ctgtgaagcg gtggcgctac
    19381 tcattaaggc aacaaaatga ttgagtttga taatctcact tacctccacg gtaaaccgca
    19441 aggcaccggg ctgctgaaag ccaatccgga agactttgtg gtggtggaag atttgggctt
    19501 tgagcctgat ggtgaaggtg agcatattct ggttagaatc ctcaaaaacg gctgcaatac
    19561 ccgttttgtg gcggatgcac tggcgaaatt cctgaaaatt cattcccgtg aagtcagctt
    19621 cgctgggcaa aaagacaaac atgctgtaac ggaacagtgg ttatgtgctc gcgtgccggg
    19681 caaggaaatg cccgatctga gcgcctttca actggaaggc tgccaggtgc tggagtatgc
    19741 gcggcacaag cgcaagctgc gtttaggggc gctgaaaggt aacgccttta ccctggttct
    19801 gcgcgaagtg agcaatcgcg atgacgttga acaacgtctg atcgatattt gcgtaaaagg
    19861 tgtaccgaac tacttcggtg cccaacgttt tgggattggc ggtagcaact tgcagggcgc
    19921 gctgcgctgg gcgcaaacca atactccggt gcgcgatcgc aataaacgga gtttttggtt
    19981 gtcggcagcc cgcagtgcgt tgtttaatca gattgttgct gagcgcctca aaaaagcaga
    20041 cgttaatcaa gttgttgacg gcgatgcgct acaattagcc ggacgtggta gctggtttgt
    20101 agcaaccacc gaagaactgg cggaattaca gcgtcgcgtc aacgataaag agctgatgat
    20161 aaccgccgca ttgccgggca gtggcgaatg gggaactcag cgtgaagcgc tggcattcga
    20221 acaagcagct gtcgccgcag aaactgaatt acaagcttta ctggtgcgcg aaaaagttga
    20281 agccgcgcgc agagcgatgc tgctgtatcc gcaacaatta agctggaact ggtgggatga
    20341 cgtcaccgta gagatccgtt tctggcttcc ggcgggtagt tttgcaacca gcgttgtcag
    20401 ggaacttatc aacacaacag gtgattatgc gcatattgct gagtaattat gacggggtac
    20461 atgcacccgg tatacaaacg ctggcgaaag ccttgcgtga gtttgctgac gttcaggtgg
    20521 tcgcccccga tcgtaaccgc agcggcgctt caaattctct gacactggaa tcctccctgc
    20581 gcacgtttac ctttgaaaat ggtgatattg ctgtgcaaat gggaaccccg accgattgtg
    20641 tctatcttgg cgtgaatgct ctgatgcgtc cgcgcccgga cattgttgtg tccggaatta
    20701 acgccgggcc gaatctgggg gatgatgtta tttattccgg tacggtagcc gccgcgatgg
    20761 aaggccgtca tttaggtttt ccggcgctgg ccgtctcgct tgacgggcat aaacattacg
    20821 acactgccgc ggcggtaacc tgttcaattt tgcgcgcact gtgtaaagag ccgctgcgca
    20881 ccggacgtat tcttaatatt aacgttcagg atttaccctt ggatcaaatc aaaggtattc
    20941 gcgtgacgcg ctgcggtaca cgacatccgg cagatcaggt gatcccgcag caagatccgc
    21001 gcggcaatac gctgtactgg attggcccgc cgggcggtaa atgtgatgcc ggtccgggga
    21061 ccgattttgc tgcggtagat gagggctatg tctccatcac gccgctgcat gtggatttaa
    21121 ctgcgcatag cgcgcaagat gtggtttcag actggttaaa cagcgtggga gttggcacgc
    21181 aatggtaagc agacgcgtac aagcacttct ggatcaatta cgtgcgcaag gtattcagga
    21241 tgagcaggtg ctgaatgcac ttgccgccgt gccgcgtgaa aaattcgttg atgaagcgtt
    21301 tgaacaaaaa gcctgggaca atatcgcctt gccgataggt caggggcaga caatttcgca
    21361 gccgtatatg gtggcgcgaa tgaccgaatt actcgagctg acgccgcagt cgcgggtgct
    21421 ggaaattggc actggttcgg gatatcaaac ggcaatcctg gcgcatcttg tccagcatgt
    21481 ttgctcggtt gaacggatta aaggcttgca gtggcaggca cgtcgccgcc tgaaaaatct
    21541 tgatttacat aatgtttcaa cccgtcatgg tgatggatgg caaggttggc aggcacgtgc
    21601 gccgtttgac gctatcattg ttacggcggc accgccggaa attccaactg cgctaatgac
    21661 gcagctggac gaaggcggga ttctcgtctt acccgtaggg gaggagcacc agtatttgaa
    21721 acgggtgcgt cgtcggggag gcgaatttat tatcgatacc gtggaggccg tgcgctttgt
    21781 ccctttagtg aagggtgagc tggcttaaaa cgtgaggaaa tacctggatt tttcctggtt
    21841 attttgccgt aggtcagcgt atcgtgaaca tcttttccag tgttcagtag ggtgccttgc
    21901 acggtaatta tgtcactggt tattaaccaa tttttcctgg gggataaatg agcgcgggaa
    21961 gcccaaaatt caccgttcgc cgcattgcgg ctttgtcact ggtttcgcta tggctggcag
    22021 gctgttctga cacttcaaat ccaccggcac cggtcagctc cgttaatggc aatgcgcctg
    22081 caaatactaa ttctggtatg ttgattacgc cgccgccgaa aatggggacg acgtctacag
    22141 cgcagcaacc gcaaattcag ccggtacagc agccacaaat tcaggctact caacaaccgc
    22201 aaatccagcc agtgcagcca gtagctcagc agccggtaca gatggaaaac ggacgcatcg
    22261 tctataaccg tcagtatggg aacattccga aaggcagtta tagcggcagt acctataccg
    22321 tgaaaaaagg cgacacactt ttctatatcg cctggattac tggcaacgat ttccgtgacc
    22381 ttgctcagcg caacaatatt caggcaccat acgcgctgaa cgttggtcag accttgcagg
    22441 tgggtaatgc ttccggtacg ccaatcactg gcggaaatgc cattacccag gccgacgcag
    22501 cagagcaagg agttgtgatc aagcctgcac aaaattccac cgttgctgtt gcgtcgcaac
    22561 cgacaattac gtattctgag tcttcgggtg aacagagtgc taacaaaatg ttgccgaaca
    22621 acaagccaac tgcgaccacg gtcacagcgc ctgtaacggt accaacagca agcacaaccg
    22681 agccgactgt cagcagtaca tcaaccagta cgcctatctc cacctggcgc tggccgactg
    22741 agggcaaagt gatcgaaacc tttggcgctt ctgagggggg caacaagggg attgatatcg
    22801 caggcagcaa aggacaggca attatcgcga ccgcagatgg ccgcgttgtt tatgctggta
    22861 acgcgctgcg cggctacggt aatctgatta tcatcaaaca taatgatgat tacctgagtg
    22921 cctacgccca taacgacaca atgctggtcc gggaacaaca agaagtgaag gcggggcaaa
    22981 aaatagcaac catgggtagc accggaacca gttcaacacg cttgcatttt gaaattcgtt
    23041 acaaggggaa atccgtaaac ccgctgcgtt atttgccgca gcgataaatc ggcggaacca
    23101 ggcttttgct tgaatgttcc gtcaagggat cacgggtagg agccacctta tgagtcagaa
    23161 tacgctgaaa gttcatgatt taaatgaaga tgcggaattt gatgagaacg gagttgaggt
    23221 ttttgacgaa aaggccttag tagaagagga acccagtgat aacgatttgg ccgaagagga
    23281 actgttatcg cagggagcca cacagcgtgt gttggacgcg actcagcttt accttggtga
    23341 gattggttat tcaccactgt taacggccga agaagaagtt tattttgcgc gtcgcgcact
    23401 gcgtggagat gtcgcctctc gccgccggat gatcgagagt aacttgcgtc tggtggtaaa
    23461 aattgcccgc cgttatggca atcgtggtct ggcgttgctg gaccttatcg aagagggcaa
    23521 cttggggctg atccgcgcgg tagagaagtt tgacccggaa cgtggtttcc gcttctcaac
    23581 atacgcaacc tggtggattc gccagacgat tgaacgggcg attatgaacc aaacccgtac
    23641 tattcgtttg ccgattcaca tcgtaaagga gctgaacgtt tacctgcgaa ccgcacgtga
    23701 gttgtcccat aagctggacc atgaaccaag tgcggaagag atcgcagagc aactggataa
    23761 gccagttgat gacgtcagcc gtatgcttcg tcttaacgag cgcattacct cggtagacac
    23821 cccgctgggt ggtgattccg aaaaagcgtt gctggacatc ctggccgatg aaaaagagaa
    23881 cggtccggaa gataccacgc aagatgacga tatgaagcag agcatcgtca aatggctgtt
    23941 cgagctgaac gccaaacagc gtgaagtact ggcacgtcga ttcggtttgc tggggtatga
    24001 agcggcaaca ctggaagatg taggtcgtga aattggcctc acccgtgaac gtgttcgcca
    24061 gattcaggtt gaaggcctgc gccgtttgcg cgaaatcctg caaacgcagg ggctgaatat
    24121 cgaagcgctg ttccgcaagt aagtaagcat ctgtcagaaa ggccagtctc aagcgaggct
    24181 ggcctttttc ttttgtttgg gaagcttgtg tttttgtcgc ctggataaga cacgtcagcg
    24241 tcctacccgg caatcagcat tctatgtgct catcatcttg cgcaccaact gcataaacag
    24301 ctcctgctct tctccactca aacgccccaa aaattcctca tccacgctgt caccaatagg
    24361 tatcgccgca gcaagtagct tttctccttc ggcagtcagc cagacaaagc gtcgccgctt
    24421 gtcagcgacg tcatgttcac gtctgactaa gccacgattt tccattcttg ccaacatttc
    24481 tgctagcgtt gctttggtgc tgactgctgc ttctatcagt gccacctgtt caataccagg
    24541 tttatcagca atggcgcgca tcaccgcgta ctgtggtttg gtcaggtcag gtaactcgtg
    24601 ctgccagcga gccgtgtgct gctgaaaaag ctgtcgtaac tgatggaacg ctttatttcg
    24661 taacgccatg taaactccgg gctaattctc tgtcgctatc atagcggttt tttagtgacg
    24721 aagaagatgt tgttactttt caatccttgc cgctgtggaa atgaaggagt atgttaataa
    24781 taacgttcgt atacgaataa ttaagagaat aagcaatgaa actgatcgtc gggatgacag
    24841 gggctaccgg tgcgcctctt ggtgtggcat tactgcaagc gctgcgtgag atgccgaatg
    24901 tcgagactca tctggtgatg tcgaagtggg cgaaaaccac cattgaactg gaaacgcctt
    24961 acagcgctcg cgatgttgct gccctcgcag acttcagcca taacccggcg gatcaggcgg
    25021 cgatcatctc atccggttct tttcgtaccg acggcatgat cgttattccg tgcagtatga
    25081 aaacgctcgc cggtatccgc gctggttacg ccgatggcct ggtagggcgc gcggcggacg
    25141 tcgtgctcaa agaaggccgc aaactggtgc tggtgccgcg tgaaatgccg cttagcacca
    25201 tccatctcga aaatatgctc gcactttcac gcatgggcgt ggcgatggtg ccgccgatgc
    25261 ctgcctttta taaccatccc gaaacggtag atgacattgt ccaccatgtg gtagcccgcg
    25321 tgctggatca atttggcctc gaacatcccc acgccaggcg ctggcaagga ttgccgcagg
    25381 cccggaattt ttctcaggag aatgaataat ggcatttgat gatttacgca gctttttaca
    25441 ggcgcttgat gaccacggcc agttactgaa aatcagcgaa gaagtgaacg ctgagccgga
    25501 tctggcagca gcagctaacg ccaccgggcg tatcggcgac ggcgcgcccg cgctgtggtt
    25561 tgataatatt cgcggcttta ccgatgcccg cgtggcgatg aataccatcg gttcctggca
    25621 gaaccacgcg atttcccttg gcctgccacc aaacaccccg gtcaaaaagc agattgatga
    25681 gtttatccgc cgctgggata acttcccgat tgccccggag cgccgcgcca atccagcctg
    25741 ggcgcagaac accgtcgatg gcgaagagat caacctgttc gatattctgc cgctgtttcg
    25801 cctgaatgat ggcgatggcg gtttctatct cgacaaagcg tgcgtggttt cccgcgatcc
    25861 gctcgacccg gataacttcg gcaagcagaa cgtcggcatc taccgcatgg aagtgaaggg
    25921 caagcgtaag ctcggcctgc aaccggtgcc gatgcacgat atcgccctgc atctgcataa
    25981 agcagaagag cgcggtgaag atctgccgat tgcgatcacg ctcggtaacg atccgatcat
    26041 cacgctgatg ggggccacgc cgctgaaata cgatcagtct gagtatgaaa tggcaggcgc
    26101 gctgcgtgaa agcccgtacc cgatcgccac cgctccgctg accggttttg atgtgccgtg
    26161 gggttcagaa gtgatcctcg aaggggttat cgaaagccgt aaacgcgaaa tcgaagggcc
    26221 gttcggtgag tttaccgggc actactccgg tgggcgcaac atgaccgtgg tgcgcatcga
    26281 taaagtctct tatcgcagca aaccgatttt cgaatcactc tatctcggta tgccgtggac
    26341 cgaaatcgac tacctatgac taggtaattt attcaagata accgggaaga gattaaaaaa
    26401 gacgggaata gattggaatc aacaagttag gaactcaagg ttttcaagat aaagcgagtc
    26461 caaaaatgtt aaacggttcg atatcaataa aacattcaag ataactcaca ggttcttaca
    26521 atagccttta aagaccgttc aaaaacgatt caggtgttta ttatagcgct gatgggtgca
    26581 agcgccaaaa ccaatgtaat tttagcacag ctaggcactt gcactgggtt atgcgctggc
    26641 tgctttctga ggtagattta agtgatcgcc cttagcttcg gacaacgcgt aaatctctct
    26701 aaccttttcg tctggcaaat taccaaggct taacgctatg gctctacgaa ctccaactag
    26761 agcgtgcaac tcttggatgt gctcatccag aagaatgagt aattgctccg ctccttttct
    26821 cccaagaaca tcagctactt gttgtttttc ttgtggttta agaaatgaca agaacattag
    26881 aatttgagca tcaacattat ccgcattctc ttttttgggt tctttggaat cttttagttc
    26941 aacatcaccc tctcccgtca tgagccattc caaggtaaca ttttcgcttt ctgctatctt
    27001 ttttaactga tacgggcgtg gtatcggtgc gtaatcccgc ctgttgtaat aattttttaa
    27061 agtgccttca ttgatccccc atgcccgagc agccgcattt cttgaagggt atcgttctac
    27121 taacctaaag attctttcga taatcggttc ttttccatcg gcaggaaaag aaccttgggg
    27181 tgcttttctc attcttggtt cctttctatc taattcattg ataaataata aaattaaaaa
    27241 ataaaccata aaaacaccaa agaacccttt gcagggaatc tttggtatct taatctttat
    27301 ccatacggat atccgaccgg atatccgcgc gggtaatttt gtaggttaaa ggaaagatga
    27361 gacaaagaga agatagtgac cgcgattggc cttcgctgaa gattcagctg gcgctcaaag
    27421 ataacgggtg gtcttccttc cgggagcttt cactccatgc tggttatgct caggattcac
    27481 tgaaagcggt agttcgcaca ccaaacaaaa aatacgaacg cgtaatttct gacgctatag
    27541 gtgtaccgcc agaaatcatc tggcccagcc ggtatcgtaa cgatggattt atcaggaagg
    27601 cgtcgtgata tgcagtcgta cgttaccgta aatgacctgc tcggcgtacc aggaatgcca
    27661 gccaccacaa aaggcatccg acaggcgtta cagcgttttt ctcgtgatct tggtgacgtt
    27721 agtcgccgtc gggaaggcac aaaagccatt gagtaccaca ttgattgtct gcctgaaata
    27781 acacgaaagg cactgcgtga acgctatgtg gaacagctgg tggctacaga gaacaatgtt
    27841 tctgaagtga aagctgttac cagaaaaaca cgcaatcctg acgctgtcca ggcaatcgaa
    27901 gcatatcgcg gttctccaca actgatggaa gaacgcctga atgcgctgac cgaaaaccag
    27961 cgctgggtat ctgaagcaag agctgcgctg gtggtggaag tgctgaagct ggaaagcgcc
    28021 ggtaaccccg ggcgactgaa agccattaac tttcttgttg aaaaagcccg taaaggtgag
    28081 ctgccggagc gcctgcaaca ggccgcagtt aacgccaatg caaaacgtgg cgctaatcgt
    28141 actatcagcc gcgatccact ttaccagtgg gttctgaaat acaaccagtc gcagaacgcc
    28201 gccgaacggc tgctgttact ggcaccggga aaacgcgacg aaataaaacc ggaagaaatc
    28261 agctggctgc ctgaatttct ggcgcagtat cgccaggtga acggcagacc catgtcagaa
    28321 gcctacgagg attttgtcgc agaatggcag cgtcgccatg cggacgaacc gtacatgctg
    28381 gaggtgatgc cttcctatga tgttgtccgg tatgccatga aaaaactgcc ggaagtggtg
    28441 aaacaaaaag ggcgcgttac cggcagtgaa taccgtcagc ttgaaggatt cacccgccgc
    28501 gactggacag ctatgccggt gaattatgtc tggattggtg acggtcacgg tatgaagctg
    28561 aaatgtgcgc atccgatcca cgggcgtcca ttctcaccgg aagtcacgtt tgtgatcgat
    28621 ggtggaacac ggtttgttgt tggctggagt cttgaccttg ccgaaaacgt gttcgccgtt
    28681 gccggtgcga tacagcacgg cattcgcaac cacggaaaac cgttcctgta ttactcggat
    28741 aacggttccg gtgaaacggc tgacatgctg gacaaagagg tcgtgggtat tctgccacgc
    28801 ctggggatta aacaccctac cggtattgcc ggtaatccac agggacgcgg cattattgaa
    28861 cgcctgaacc ggacactgcc gatgcgtata gccaggaggt atcgcaccta ttttggcaag
    28921 ggtgctgacc gggaatcgtt gcgtgtactt aaccgtgatc tgcgttcagc gtttaacgcc
    28981 ctgcaacagg acaagcctct gaacgaccgc cagaaagcgg caatgcgcga gctgccatcg
    29041 tgggcgtatt cgtgaaggcg tggaatggta caacaaccgc ccgcattctg agctgccgat
    29101 gaaaccgaac ggacggcatt acagcccgac agagttcaga aaaaaacgcc aggcagaaga
    29161 agacaccgaa attgagtggc tgagtgatct tgagctgcgc gacatgttcc gcccaatggt
    29221 ggaacgaccg gtaagacgct gcgaaattca gtggctgaac aatatttact acgcgccgga
    29281 gttgcgtgac gaacacggtc gcaaagtgct tatcagttat gacattcatg acgccgagcg
    29341 aattacggta cgtcgcaagg acggcagctt tatctgtgag gcgatatgga acggcaataa
    29401 acgcgcagcc tttgctgtca gtgctgaata tcacaaacaa cagcagcgta ttaaaggaat
    29461 gcgcaaacgc gctgaggaaa aaattcgtga tgctgaagat gaaggtattc agattctgg
//
LOCUS       DAJTRH010000044        29456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTRH010000044 DAJTRH010000000
VERSION     DAJTRH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..203)
                     /locus_tag="OO339_002107"
     CDS             complement(<1..203)
                     /locus_tag="OO339_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5926735.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALK"
     gene            513..2285
                     /gene="aspS"
                     /locus_tag="OO339_002108"
     CDS             513..2285
                     /gene="aspS"
                     /locus_tag="OO339_002108"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5926736.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2403..2855
                     /gene="nudB"
                     /locus_tag="OO339_002109"
     CDS             2403..2855
                     /gene="nudB"
                     /locus_tag="OO339_002109"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5926737.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2884..3624
                     /locus_tag="OO339_002110"
     CDS             2884..3624
                     /locus_tag="OO339_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5926738.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3659..4180
                     /gene="ruvC"
                     /locus_tag="OO339_002111"
     CDS             3659..4180
                     /gene="ruvC"
                     /locus_tag="OO339_002111"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5926739.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4182..4784)
                     /locus_tag="OO339_002112"
     CDS             complement(4182..4784)
                     /locus_tag="OO339_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5926740.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4854..4919
                     /gene="yobI"
                     /locus_tag="OO339_002113"
     CDS             4854..4919
                     /gene="yobI"
                     /locus_tag="OO339_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5926741.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5058..5669
                     /gene="ruvA"
                     /locus_tag="OO339_002114"
     CDS             5058..5669
                     /gene="ruvA"
                     /locus_tag="OO339_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5926742.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5678..6688
                     /gene="ruvB"
                     /locus_tag="OO339_002115"
     CDS             5678..6688
                     /gene="ruvB"
                     /locus_tag="OO339_002115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5926743.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6835..7620)
                     /gene="znuB"
                     /locus_tag="OO339_002116"
     CDS             complement(6835..7620)
                     /gene="znuB"
                     /locus_tag="OO339_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5926744.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7617..8372)
                     /gene="znuC"
                     /locus_tag="OO339_002117"
     CDS             complement(7617..8372)
                     /gene="znuC"
                     /locus_tag="OO339_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5926745.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8451..9383
                     /gene="znuA"
                     /locus_tag="OO339_002118"
     CDS             8451..9383
                     /gene="znuA"
                     /locus_tag="OO339_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5926746.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9399..10721
                     /gene="mepM"
                     /locus_tag="OO339_002119"
     CDS             9399..10721
                     /gene="mepM"
                     /locus_tag="OO339_002119"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5926747.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10841..11812
                     /gene="lpxM"
                     /locus_tag="OO339_002120"
                     /gene_synonym="msbB"
     CDS             10841..11812
                     /gene="lpxM"
                     /locus_tag="OO339_002120"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5926748.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11943..13385)
                     /gene="pyk"
                     /locus_tag="OO339_002121"
     CDS             complement(11943..13385)
                     /gene="pyk"
                     /locus_tag="OO339_002121"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5926749.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13513..14382)
                     /gene="hexR"
                     /locus_tag="OO339_002122"
                     /gene_synonym="yebK"
     CDS             complement(13513..14382)
                     /gene="hexR"
                     /locus_tag="OO339_002122"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5926750.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14719..16194
                     /gene="zwf"
                     /locus_tag="OO339_002123"
     CDS             14719..16194
                     /gene="zwf"
                     /locus_tag="OO339_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5926751.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16429..18240
                     /gene="edd"
                     /locus_tag="OO339_002124"
     CDS             16429..18240
                     /gene="edd"
                     /locus_tag="OO339_002124"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5926752.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18277..18918
                     /gene="kdgA"
                     /locus_tag="OO339_002125"
                     /gene_synonym="eda"
     CDS             18277..18918
                     /gene="kdgA"
                     /locus_tag="OO339_002125"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5926753.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18974..20152)
                     /gene="purT"
                     /locus_tag="OO339_002126"
     CDS             complement(18974..20152)
                     /gene="purT"
                     /locus_tag="OO339_002126"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5926754.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20286..20576
                     /gene="yebG"
                     /locus_tag="OO339_002127"
                     /pseudo
     CDS             20286..20576
                     /gene="yebG"
                     /locus_tag="OO339_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20643..20999
                     /locus_tag="OO339_002128"
     CDS             20643..20999
                     /locus_tag="OO339_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5926755.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21326..21985
                     /locus_tag="OO339_002129"
     CDS             21326..21985
                     /locus_tag="OO339_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5926756.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22195..24255
                     /gene="ptrB"
                     /locus_tag="OO339_002130"
     CDS             22195..24255
                     /gene="ptrB"
                     /locus_tag="OO339_002130"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5926757.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24252..24914)
                     /gene="exoX"
                     /locus_tag="OO339_002131"
     CDS             complement(24252..24914)
                     /gene="exoX"
                     /locus_tag="OO339_002131"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5926758.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24938..25594)
                     /locus_tag="OO339_002132"
                     /pseudo
     CDS             complement(24938..25594)
                     /locus_tag="OO339_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25696..25926)
                     /gene="holE"
                     /locus_tag="OO339_002133"
     CDS             complement(25696..25926)
                     /gene="holE"
                     /locus_tag="OO339_002133"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5926759.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26065..26439
                     /gene="yobA"
                     /locus_tag="OO339_002134"
     CDS             26065..26439
                     /gene="yobA"
                     /locus_tag="OO339_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5926760.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26443..27315
                     /gene="copD"
                     /locus_tag="OO339_002135"
                     /pseudo
     CDS             26443..27315
                     /gene="copD"
                     /locus_tag="OO339_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27328..27669
                     /locus_tag="OO339_002136"
     CDS             27328..27669
                     /locus_tag="OO339_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5926761.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28062..29139)
                     /locus_tag="OO339_002137"
                     /pseudo
     CDS             complement(28062..29139)
                     /locus_tag="OO339_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(29105..29386)
                     /locus_tag="OO339_002138"
     CDS             complement(29105..29386)
                     /locus_tag="OO339_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5926762.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7434 a         7231 c         7566 g         7225 t
ORIGIN      
        1 tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa cacgggctga
       61 ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta
      121 tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac caacgcagtg
      181 gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg cctcacacct
      241 taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac
      301 acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg
      361 cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt
      421 ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg
      481 catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt ggacagctcc
      541 gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc
      601 ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg
      661 atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag ttctgcattc
      721 aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg
      781 gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat gttctgccgc
      841 ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac ctcgacctgc
      901 gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc
      961 gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc aaagccacgc
     1021 cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcac
     1081 tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt gaccgttact
     1141 atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta
     1201 ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag
     1261 cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat ttcccggtaa
     1321 tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg cgtaacccga
     1381 tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta tttgcggggc
     1441 cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc gcatcgctga
     1501 cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg
     1561 cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga
     1621 agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg caagatggcg
     1681 atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg ggcgcactgc
     1741 tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca ccgctgtggg
     1801 ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca atgcaccatc
     1861 cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg gaaaatgcgg
     1921 tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt tcagtacgta
     1981 tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac gaagaggaac
     2041 agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg ccgcacgcag
     2101 gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac aatatccgtg
     2161 acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa gcaccgagct
     2221 ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag gctgagaata
     2281 actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca actgagtgaa
     2341 ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa cgaagaggca
     2401 gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc tatgcacaag
     2461 atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg cagtcggtaa
     2521 ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag
     2581 aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag cgcacggtag
     2641 agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg cgtaatacgg
     2701 aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact gaacatctgg
     2761 cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg agcaaccggc
     2821 aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc tggagatatt
     2881 tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc gcaggatgct
     2941 aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc taagctgggc
     3001 ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc actgtctaac
     3061 aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat
     3121 gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc aatcatgatt
     3181 gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc attcagcaaa
     3241 tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa aaaaggcgtg
     3301 atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga agcaggtgct
     3361 gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg ggaagaaatg
     3421 ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc
     3481 atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact gatgcgtctg
     3541 atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg tgaaatctct
     3601 gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga gacgcgtgat
     3661 ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg tcatccgcca
     3721 ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag tggatgattt
     3781 accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc agttccagcc
     3841 tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag ccctgaaact
     3901 gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc cggtatttga
     3961 atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg aaaaaagcca
     4021 ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg cggatgccgc
     4081 cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga tgcagatgag
     4141 cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc ctcggtcagt
     4201 tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg atgatgccac
     4261 aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa ccagaatttc
     4321 cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt ctctatttca
     4381 cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga acagaactgg
     4441 cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga gggaacctgt
     4501 tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt tatagcatag
     4561 cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga aaggggaacc
     4621 cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc gacgtgatta
     4681 ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat acatgtaaag
     4741 acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc attccattga
     4801 aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc gcaatgtata
     4861 tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt ccaatataac
     4921 ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg caagttttct
     4981 tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat ctttgattca
     5041 ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa aaacaacccc
     5101 cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg atgacctgtt
     5161 tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg
     5221 aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg ttcaaagagt
     5281 tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc ggaatgtcag
     5341 cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg aaactgccgg
     5401 gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt
     5461 tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct gctagcccgg
     5521 cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc tataaaccac
     5581 aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt gaaacattaa
     5641 ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag accgtctgat
     5701 ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc ctaaattact
     5761 ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca tcaaagcagc
     5821 gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg ggttgggtaa
     5881 aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca cgacttctgg
     5941 tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg aaccgcatga
     6001 cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag tgctgtaccc
     6061 ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg cacgctccat
     6121 taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag gttcgctgac
     6181 atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc aggtgccgga
     6241 tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga gtgatgacgg
     6301 tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg
     6361 tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag atatcgctgc
     6421 tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg accgcaaatt
     6481 gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata acctggcggc
     6541 agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt tgattcagca
     6601 aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt ggaatcactt
     6661 tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg catccgacac
     6721 tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga gatagcgaac
     6781 cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga tagattagct
     6841 ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca ccaccgacgg
     6901 acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg ccaccatccc
     6961 caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc
     7021 tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca tcgctacacc
     7081 aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt tcacaccatc
     7141 aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa
     7201 aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg gcgtcactgc
     7261 cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca tcagactaac
     7321 gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg tgtcgatcgc
     7381 cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg taacggcaat
     7441 caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc
     7501 atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg aacccagcgg
     7561 acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata attcaatcat
     7621 gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg atgattatga
     7681 tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga aataaactcc
     7741 ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt caggcacagc
     7801 acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa aacgccacaa
     7861 tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc atttacatcc
     7921 acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa tgctcgcgct
     7981 aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt aatcaggtgc
     8041 ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc agggcgtaag
     8101 cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt ctgcggtaca
     8161 tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt taccagcccg
     8221 agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa agtcaaaatt
     8281 tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc aaaagaaacc
     8341 gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca gtagtcatga
     8401 aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt atgttacata
     8461 aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca acacaggccg
     8521 cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct gccattgctg
     8581 atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat gattattcac
     8641 tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg gttggcccgg
     8701 agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag caggtaacga
     8761 ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc gatgatgatg
     8821 atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc aacatgcatc
     8881 tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga aaattagtgg
     8941 aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt gaggcacaat
     9001 tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg aaaggttatt
     9061 tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca ccgcttggtc
     9121 attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa ataagaacac
     9181 agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg ccagcggtcg
     9241 ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc ctggggacga
     9301 atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc aaccagtatg
     9361 cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata gcccgctctg
     9421 tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg
     9481 ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat gccacgccaa
     9541 ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct gaagccagtg
     9601 agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa ctggatgaca
     9661 aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat acgctaagca
     9721 gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct gctgccgaca
     9781 aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc gcggacggcg
     9841 aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg
     9901 ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt aacaatctgc
     9961 tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt ttaaccagcg
    10021 ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc aaactgaaaa
    10081 aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa
    10141 gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca atccgcgctg
    10201 aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc ttgcgattcc
    10261 cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact aatccggtga
    10321 caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt acgccagtgc
    10381 tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca ggttattatg
    10441 tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc aagattctgg
    10501 tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt aataccggac
    10561 gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc gtaaacccgc
    10621 tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc gaattcctgg
    10681 cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc attcgcagcc
    10741 ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc cgctacacta
    10801 tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa
    10861 tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact ggggagcatg
    10921 gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc gtgatcccat
    10981 tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt
    11041 aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag cgattgttga
    11101 tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg
    11161 gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg aagagatgcg
    11221 gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg atattcctgc
    11281 catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc agggcaaccc
    11341 ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag
    11401 aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg gatattattt
    11461 acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg ccacctataa
    11521 agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg ttgtaccgct
    11581 gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc caccgatgga
    11641 tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag aagtcgagat
    11701 ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga aaactcgcaa
    11761 accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat aaaaaagcct
    11821 ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa tattcgaaat
    11881 ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat ccggcaacgt
    11941 acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact catcacgtcg
    12001 ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg cagcagatta
    12061 accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac cggcgtaacg
    12121 ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc gaaaattggt
    12181 agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt catggtaatg
    12241 atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc aatagcttct
    12301 tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct cgggattttt
    12361 tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata ctgcccagcg
    12421 gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt tgctacgtcc
    12481 atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat ctgggtcgcc
    12541 gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg aatgccgacc
    12601 agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac cacgtcagag
    12661 gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg ttcaaccttg
    12721 gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata gttcagatct
    12781 tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc cgcagtctta
    12841 atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc aagtttgttg
    12901 atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac tttcatgccc
    12961 tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat gtcaccaggc
    13021 acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc gccttcacct
    13081 ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac tttgccttct
    13141 ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc cacatgacgc
    13201 cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc ttcaggcgag
    13261 ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt ttcaagatta
    13321 ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg aagccttttg
    13381 gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga aatccgaatg
    13441 aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag attaggacag
    13501 tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa aacgcgattc
    13561 cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc gtcgcaaagt
    13621 aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa ccatgggcat
    13681 ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt cccgggcgag
    13741 cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg ccagctgcgc
    13801 cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca cgtctccgtc
    13861 gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca ccaccggaac
    13921 attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag agcctaatcc
    13981 gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga tggcagattt
    14041 atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata ttttccccgt
    14101 gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag tgccattcgc
    14161 cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca tgctgcgaca
    14221 gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag caatactcga
    14281 atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct ctgatttgct
    14341 caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa gagactcacg
    14401 ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga tattacgcct
    14461 gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca taacatgatc
    14521 agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg ccgcgctttg
    14581 cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca agctcgtaaa
    14641 agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta ccgggttagt
    14701 taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga cctggtcatt
    14761 ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta tcaactggaa
    14821 aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc tgactgggat
    14881 aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa agaaaccatt
    14941 gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct cgatgtcaat
    15001 gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg tatcactatt
    15061 aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct tggcgaggca
    15121 aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac gtcgctggcg
    15181 acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca ggtttaccgt
    15241 atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg ttttgctaac
    15301 tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat taccgtggca
    15361 gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca gatgcgtgac
    15421 atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc gccatctgac
    15481 ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct gcgccgcatc
    15541 gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg cttcgcccag
    15601 ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag caatacagaa
    15661 accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt gccattctac
    15721 ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta tttcaaaaca
    15781 cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa actgactatc
    15841 cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc tggccttgac
    15901 cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac ctttaatcag
    15961 acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg tattcaggca
    16021 ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc cattactgag
    16081 gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg gggacccgtt
    16141 gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga gtaatatcag
    16201 cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg tagaacaatt
    16261 tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta cactgactga
    16321 aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc tctgaatgaa
    16381 acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat gaatccacaa
    16441 ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc tgcttatctc
    16501 gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc atgcggtaac
    16561 ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa aagcatgttg
    16621 cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca ccagccttat
    16681 gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt tggtcaggtt
    16741 gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg aatggaattg
    16801 tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc ccataacatg
    16861 tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct gacgatggca
    16921 gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat ggcaagcggt
    16981 ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa agtggaccgc
    17041 atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg tactttctac
    17101 ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt gccaggctct
    17161 tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc tgcgcgtcag
    17221 gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat gatcgatgag
    17281 aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac taaccacacc
    17341 atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga tgacttctct
    17401 gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc ggccgatatt
    17461 aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct caaagcaggc
    17521 ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta tacccttgaa
    17581 ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc actcgacagc
    17641 aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa agtgttaagc
    17701 ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa ccaggtgatt
    17761 gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt tgaagcgggt
    17821 ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc gaacggaatg
    17881 ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg tttcaaaatt
    17941 gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc agctatccac
    18001 gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg ggacatcatt
    18061 cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga actggctgct
    18121 cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg tgaattattc
    18181 agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat cactttttaa
    18241 gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa aacaagtgca
    18301 gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa actggaacac
    18361 gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga agtgactctg
    18421 cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc tgaagcgatt
    18481 gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga agcgggtgca
    18541 cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac cgaagggact
    18601 attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat ggactacggt
    18661 ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc cctgcaggcg
    18721 atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc tccggctaac
    18781 taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg gctggttccg
    18841 gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg tgaagctgta
    18901 gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt tttgtagcgt
    18961 ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc gcgcgttcaa
    19021 tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg ctgccatcaa
    19081 tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc tgcacattat
    19141 caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct gcaggaccat
    19201 actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg gcaaactctg
    19261 agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg gagacctcac
    19321 tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca taaccgccca
    19381 gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc gggctcattt
    19441 gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct actggtgcac
    19501 agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag tcaaacttaa
    19561 cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga gcgtattccc
    19621 atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg gaagagctca
    19681 tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg aaaaggcttt
    19741 tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc gccaggcgac
    19801 ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca acattcagtc
    19861 cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc ggcacgatat
    19921 aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc atattaatga
    19981 catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg acggcaatca
    20041 cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca ccggagccta
    20101 ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc ataacgaata
    20161 tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc tttattgctg
    20221 atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat tatgagaggc
    20281 tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag aaaatgtcgt
    20341 ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg gatctgcttg
    20401 acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa gccctttcgc
    20461 tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc aaattaccgt
    20521 ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg gcctgaagca
    20581 acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct atggagaaaa
    20641 acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc gcatcagttt
    20701 tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa ggtctggatg
    20761 ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg gcgcgctggg
    20821 cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt ttgcaggaca
    20881 ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa agtgccgata
    20941 ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag cggcgttaat
    21001 ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg tccgtcccag
    21061 aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc aatcagcatc
    21121 aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat cacctgatat
    21181 acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc cccgcttatt
    21241 tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg ctacaggcac
    21301 taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc aatccctgct
    21361 tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca aactgttagt
    21421 gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag cacgtaaact
    21481 tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag cgggtacggt
    21541 gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac cgcagtttgg
    21601 cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc tggtctttgc
    21661 cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc agcaattgcg
    21721 tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg aacaaccgct
    21781 ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg aaatctattt
    21841 cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc tgaatgcact
    21901 gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg atctcgaaca
    21961 gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt tgactatttc
    22021 gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct gcataacatt
    22081 cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt cgcttgcatc
    22141 cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa taacatgcta
    22201 ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg catcgataat
    22261 tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta cctgcaacaa
    22321 gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga tcgcatctta
    22381 aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat aaaaaatggc
    22441 taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca gcgtcaatcg
    22501 gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa gcgcgcagct
    22561 catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac cattatggcg
    22621 ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa tctggaaaca
    22681 ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg ggcaaatgac
    22741 tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta tcaggtctgg
    22801 cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga aaaagacgat
    22861 acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat tcatctggcc
    22921 agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc cgagccgttt
    22981 gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca gcatcgtttt
    23041 tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg tatgcgtgat
    23101 gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga agggtttacg
    23161 ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa
    23221 attaaccgca agacccggga agtcattggc attgcctttg atgatccggc ctatgtgacc
    23281 tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta ttcttccatg
    23341 accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg tgtattaaaa
    23401 caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct gtggatagtc
    23461 gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca ttttcgcaaa
    23521 ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat tgatgccgat
    23581 ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat tgtccatgtt
    23641 cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct gaagaagaaa
    23701 aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg ctatggctct
    23761 ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg cgttgcaatt
    23821 aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt tgatgttgta
    23881 acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga gtggggaaat
    23941 ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga caacgtcacc
    24001 gcacaggctt atccgcattt actggtaacg accggtttac acgattctca ggtgcaatat
    24061 tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga ccatctttta
    24121 ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt taaatcgtac
    24181 gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct
    24241 acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc
    24301 atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc ggcaacgtcg
    24361 gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga cggacgtccg
    24421 gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac
    24481 gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag cggtgtctgt
    24541 acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc tggccacaaa
    24601 cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg
    24661 tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct
    24721 tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt
    24781 ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat ttttccgtca
    24841 atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg
    24901 ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga atgatatcgc
    24961 cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa
    25021 cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt gccatcagca
    25081 cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg
    25141 gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata tccatacctt
    25201 gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac
    25261 tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa
    25321 agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag gtcgttgcgg
    25381 cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca cgtcgcgaat
    25441 aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg
    25501 cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct
    25561 catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc
    25621 aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt
    25681 aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gattgaccga
    25741 agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt ctggctgttc
    25801 acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag ccaccccggc
    25861 cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt
    25921 cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gcctggatgg
    25981 caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag
    26041 ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg
    26101 gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat
    26161 cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac tttaaacttc
    26221 tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa aaacgaaaat
    26281 attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat tgttccactg
    26341 gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc ggtggacggg
    26401 cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc gtttacctgg
    26461 atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt tgcaatgtat
    26521 ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc tctgcgttta
    26581 caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc cgttcagggc
    26641 gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca ggctgttttg
    26701 caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt aacgttgatc
    26761 gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa
    26821 tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac agcgaaaatc
    26881 catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg gggattactg
    26941 ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc tattcaggcg
    27001 ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg
    27061 cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg gggccaactc
    27121 ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc taatcgttat
    27181 gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg
    27241 actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct gcttgcgact
    27301 ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta
    27361 ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag tcgttttgaa
    27421 gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg ccgaccgggt
    27481 aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa cgatatcgca
    27541 caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat tcagattgat
    27601 gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa
    27661 ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc tcagttcttt
    27721 caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt tattgaccat
    27781 tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca aggcaactaa
    27841 gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt tccctggacc
    27901 gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt agttgcaaac
    27961 gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt ttttggttat
    28021 atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag taagtacgtt
    28081 ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc aagtagacgc
    28141 tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct tctgatttcg
    28201 tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg ctgcgacaaa
    28261 tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc taatcccggc
    28321 actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg caccaagtcg
    28381 taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc ctgtcttaat
    28441 ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg ttatgtcttc
    28501 gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg ccggaagttg
    28561 atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc ttgtaactac
    28621 tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt caacaatggc
    28681 ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt ttttccctcc
    28741 ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg tcagtaaaac
    28801 ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat aagtatttct
    28861 ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg ccatagtaaa
    28921 tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt tgtatatata
    28981 atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac ctagtgtaaa
    29041 ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc gatatgttaa
    29101 gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc tccattaacg
    29161 gactgtctct cccttgtcgc ccattaggct gatggtgtgt tatatcggta tcaaccttat
    29221 ttgggttgat atagaaagcc tccggaacca ccctgtaact cctcccgtgt agttcaggtg
    29281 caggataaat gtttccattc cttgcccatc gtctcagcgt tgatattgat ggtgggttat
    29341 ccggatatct gagttttccc cacgttttga gtgtcacaag attcattgcc atacctctca
    29401 cgatatgacc gccagtaaat atacagaata ctggcgggtg tggttgattt ttaata
//
LOCUS       DAJTRH010000045        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTRH010000045 DAJTRH010000000
VERSION     DAJTRH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO339_002139"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO339_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5926763.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO339_002140"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO339_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5926764.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO339_002141"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO339_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5926765.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO339_002142"
     CDS             4843..5082
                     /locus_tag="OO339_002142"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926766.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO339_002143"
     CDS             5092..5262
                     /locus_tag="OO339_002143"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5926767.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO339_002144"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO339_002144"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5926768.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO339_002145"
     CDS             6724..6990
                     /locus_tag="OO339_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5926769.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO339_002146"
     CDS             7084..7773
                     /locus_tag="OO339_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5926770.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO339_002147"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO339_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5926771.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO339_002148"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO339_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5926772.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO339_002149"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO339_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5926773.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO339_002150"
     CDS             complement(10475..12193)
                     /locus_tag="OO339_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5926774.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO339_002151"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO339_002151"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5926775.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO339_002152"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO339_002152"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5926776.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO339_002153"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO339_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5926777.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO339_002154"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO339_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5926778.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO339_002155"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO339_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO339_002156"
     CDS             18432..18746
                     /locus_tag="OO339_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5926779.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO339_002157"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO339_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5926780.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO339_002158"
     CDS             20570..21862
                     /locus_tag="OO339_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5926781.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO339_002159"
     CDS             complement(22016..23665)
                     /locus_tag="OO339_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5926782.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO339_002160"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO339_002160"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5926783.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO339_002161"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO339_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5926784.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO339_002162"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO339_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5926785.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO339_002163"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO339_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO339_002164"
     CDS             28753..29034
                     /locus_tag="OO339_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5926786.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7285 a         7776 c         7351 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcatta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTRH010000046        28932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTRH010000046 DAJTRH010000000
VERSION     DAJTRH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..2147)
                     /gene="yccS"
                     /locus_tag="OO339_002165"
     CDS             complement(<1..2147)
                     /gene="yccS"
                     /locus_tag="OO339_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5926787.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITVPTSGR"
     gene            complement(2157..2603)
                     /locus_tag="OO339_002166"
     CDS             complement(2157..2603)
                     /locus_tag="OO339_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5926788.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2726..4780
                     /gene="helD"
                     /locus_tag="OO339_002167"
     CDS             2726..4780
                     /gene="helD"
                     /locus_tag="OO339_002167"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5926789.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4812..5270)
                     /gene="mgsA"
                     /locus_tag="OO339_002168"
     CDS             complement(4812..5270)
                     /gene="mgsA"
                     /locus_tag="OO339_002168"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5926790.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5366..6028)
                     /locus_tag="OO339_002169"
     CDS             complement(5366..6028)
                     /locus_tag="OO339_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5926791.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6201..6614
                     /locus_tag="OO339_002170"
     CDS             6201..6614
                     /locus_tag="OO339_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5926792.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6659..6976)
                     /gene="hspQ"
                     /locus_tag="OO339_002171"
     CDS             complement(6659..6976)
                     /gene="hspQ"
                     /locus_tag="OO339_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5926793.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7034..8224)
                     /gene="rlmI"
                     /locus_tag="OO339_002172"
     CDS             complement(7034..8224)
                     /gene="rlmI"
                     /locus_tag="OO339_002172"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5926794.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8319..8597
                     /gene="yccX"
                     /locus_tag="OO339_002173"
     CDS             8319..8597
                     /gene="yccX"
                     /locus_tag="OO339_002173"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5926795.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8594..8923)
                     /gene="tusE"
                     /locus_tag="OO339_002174"
     CDS             complement(8594..8923)
                     /gene="tusE"
                     /locus_tag="OO339_002174"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5926796.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(9014..9673)
                     /gene="yccA"
                     /locus_tag="OO339_002175"
     CDS             complement(9014..9673)
                     /gene="yccA"
                     /locus_tag="OO339_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5926797.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9880..9967)
                     /locus_tag="OO339_002176"
     tRNA            complement(9880..9967)
                     /locus_tag="OO339_002176"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9931..9933),aa:Ser,seq:tga)
     gene            10393..11511
                     /gene="hyaA"
                     /locus_tag="OO339_002177"
     CDS             10393..11511
                     /gene="hyaA"
                     /locus_tag="OO339_002177"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5926798.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11508..13301
                     /gene="hyaB"
                     /locus_tag="OO339_002178"
     CDS             11508..13301
                     /gene="hyaB"
                     /locus_tag="OO339_002178"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5926799.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13320..14027
                     /gene="hyaC"
                     /locus_tag="OO339_002179"
     CDS             13320..14027
                     /gene="hyaC"
                     /locus_tag="OO339_002179"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5926800.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14024..14611
                     /locus_tag="OO339_002180"
     CDS             14024..14611
                     /locus_tag="OO339_002180"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5926801.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14608..15006
                     /gene="hyaE"
                     /locus_tag="OO339_002181"
     CDS             14608..15006
                     /gene="hyaE"
                     /locus_tag="OO339_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5926802.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            15003..15860
                     /locus_tag="OO339_002182"
     CDS             15003..15860
                     /locus_tag="OO339_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5926803.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15994..17538
                     /gene="appC"
                     /locus_tag="OO339_002183"
     CDS             15994..17538
                     /gene="appC"
                     /locus_tag="OO339_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5926804.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17550..18686
                     /gene="appB"
                     /locus_tag="OO339_002184"
     CDS             17550..18686
                     /gene="appB"
                     /locus_tag="OO339_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5926805.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18698..18790
                     /gene="cbdX"
                     /locus_tag="OO339_002185"
     CDS             18698..18790
                     /gene="cbdX"
                     /locus_tag="OO339_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5926806.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18870..20168
                     /gene="appA"
                     /locus_tag="OO339_002186"
     CDS             18870..20168
                     /gene="appA"
                     /locus_tag="OO339_002186"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5926807.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20283..22449)
                     /gene="etk"
                     /locus_tag="OO339_002187"
                     /pseudo
     CDS             complement(20283..22449)
                     /gene="etk"
                     /locus_tag="OO339_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22469..22915)
                     /gene="etp"
                     /locus_tag="OO339_002188"
                     /gene_synonym="yccY"
     CDS             complement(22469..22915)
                     /gene="etp"
                     /locus_tag="OO339_002188"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5926808.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22903..24042)
                     /locus_tag="OO339_002189"
     CDS             complement(22903..24042)
                     /locus_tag="OO339_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5926809.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24088..26184)
                     /locus_tag="OO339_002190"
     CDS             complement(24088..26184)
                     /locus_tag="OO339_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5926810.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26184..26930)
                     /locus_tag="OO339_002191"
     CDS             complement(26184..26930)
                     /locus_tag="OO339_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5926811.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGTG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26927..27571)
                     /locus_tag="OO339_002192"
     CDS             complement(26927..27571)
                     /locus_tag="OO339_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5926812.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27678..27983)
                     /locus_tag="OO339_002193"
     CDS             complement(27678..27983)
                     /locus_tag="OO339_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926813.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28425..28637)
                     /gene="cspH"
                     /locus_tag="OO339_002194"
     CDS             complement(28425..28637)
                     /gene="cspH"
                     /locus_tag="OO339_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5926814.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7004 a         7324 c         7608 g         6996 t
ORIGIN      
        1 cgcccggatg ttgggactgt aatctggcgt tgcagacgac caatctctgg cagtaatgca
       61 atcaataacc caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt
      121 tgctgcatcc gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt
      181 tgatgatgta acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca
      241 ggattagtta actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg
      301 ttaaggcaca gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg
      361 gacatatttg ataccaccga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca
      421 atacgatacg ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga
      481 tagcgacagt tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag
      541 tcaggccaga tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt
      601 acgcgaggta acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa
      661 agtgtgatga acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg
      721 ccggtaataa ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga
      781 atgccaatcg cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg
      841 gcgttatagt ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc
      901 attccggtta tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca
      961 tgacggaaga gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc
     1021 aatccgtgcg ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgtctgg
     1081 gcctgttctg attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa
     1141 aatcccagtg ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca
     1201 gcatcaatat gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac
     1261 aaaatacagc gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa
     1321 cggaacagca cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa
     1381 gagctggcac gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt
     1441 ccccgttgac cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat
     1501 gtcgccatca gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct
     1561 tcaatatcag gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag
     1621 caacgcgcca ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc
     1681 aggacgttgt accagacggc accggccagc agatacatcg gctgctgata ccagtgctca
     1741 tacagtgatg ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt
     1801 gcatagcgtt gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca
     1861 atcgcaaata gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag
     1921 cagaacagcg taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc
     1981 agcgccgctg ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac
     2041 cacggaaacg ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg
     2101 ctgttccagg tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat
     2161 tcaaaacgac gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg
     2221 cgacgaccaa ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga
     2281 atgccaatga ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac
     2341 cagccaaaga aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca
     2401 tttttgccag ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga
     2461 gacagtttag tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata
     2521 ctgaccagag tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc
     2581 agaatgttca aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg
     2641 caattgtaac gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa
     2701 taatttcgtg tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct
     2761 ggcacagcac ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga
     2821 tcgccacgaa taccttattc ctttcaatca attactggcg attcactgta agcgcggtct
     2881 ggtatggggc gagctggaat ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac
     2941 agaatggggc gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag
     3001 tggcgagatg agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac
     3061 gcgcactgga gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat
     3121 ccgccaggct ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg
     3181 ccgtgaggcg tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca
     3241 gcataaccag gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt
     3301 cgagtcttca ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct
     3361 gttagtgctg gctggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg
     3421 gttgctggcg cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa
     3481 agccgctgaa gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc
     3541 acgcacgttt catgcgttgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat
     3601 agtcagcaaa ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg
     3661 caagcaatgc agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga
     3721 aatgcagtgg tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct
     3781 tgcctctcgc ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga
     3841 aatgattgcc agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat
     3901 ggccccgtta ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc
     3961 gggccttatt catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa
     4021 gcatattctg gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc
     4081 attacgcaag caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat
     4141 ttaccgattc agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga
     4201 aggcgatcgc tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc
     4261 aaaccggttt attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa
     4321 tggagacaaa aaagccgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa
     4381 gctctctggt tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat
     4441 gaggcctgcc agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat
     4501 gaccattcat gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga
     4561 ggggagtggt ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc
     4621 accggttgag gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg
     4681 ggcacgccat cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat
     4741 actgaaaaat ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca
     4801 cctgtgcaat attacttcag acggtcagcg agataacgct gataatcggg gataagaata
     4861 tcgaccgcgt cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg
     4921 gcgaccggaa tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc
     4981 ggcacggcat ttagtggatc ccagaagaat atcaatacat caattttccc ttctgagatc
     5041 aatgcgccaa cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg
     5101 ccggtcgcgc gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc
     5161 agtaacggtt gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc
     5221 accagcgcaa tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc
     5281 cgtagttaac tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa
     5341 aagcctgcgc acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga
     5401 aagtatttcg cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga
     5461 gcacctgaga ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt
     5521 catctgccat catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc
     5581 gttcaacctc gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca
     5641 gctttaccgg aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa
     5701 aatggttagc ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga
     5761 gctgggtatt aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg
     5821 aaagatgaat cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa
     5881 tgctgtccgc accgcgcagc agagagctgg aaaccttctt gccgtcgagt accagaagat
     5941 cgacatcggt tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa
     6001 tcaaggtggt cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa
     6061 taatgtgtca aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt
     6121 tgctctcatt cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt
     6181 taaacagtct ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc
     6241 acaccattgc gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga
     6301 aatatctact cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa
     6361 cgctgctggg ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg
     6421 tcgatgtttt ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg
     6481 gcgcgaaaac cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac
     6541 gggatgccgg gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg
     6601 gtctggccaa ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt
     6661 agttacgcag acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg
     6721 atggctgttc aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg
     6781 tatgaaccgg taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa
     6841 gctcgtcatt caccgccagc tcatcgggcg acggttcaga gagcgaataa accgggtcga
     6901 tatccacgac cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga
     6961 atttgctggc aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat
     7021 tccacatttc aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt
     7081 aggtagcgat caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac
     7141 gaccggcatc aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac
     7201 cggaacagga gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt
     7261 tgatgtcttt ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg
     7321 gcgggtccat cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt
     7381 taaagacatc atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt
     7441 tctgccgtgc aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac
     7501 cgcccatcag tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt
     7561 tattttcaac gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg
     7621 ttttgtgtcc gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagca
     7681 gggcaggcgg caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac
     7741 gtaccgcaac atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac
     7801 taattaatgc cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat
     7861 taccgaaacg atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat
     7921 agctgtcgag gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac
     7981 ggcgggaaaa aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc
     8041 ggatttgcga agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa
     8101 tatcgatggt ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga
     8161 cccacggatg gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac
     8221 tcataattta cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg
     8281 gatttgcaac gcgtccatta aggataagga gagcagcaat gtcgaaaatc tgcataattg
     8341 cctggattta cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga
     8401 aaagactggg cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg
     8461 cctgcggtga agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc
     8521 gttctgcgcg tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg
     8581 attttcgtat ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt
     8641 gcttagccgg gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac
     8701 caaatttatt cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca
     8761 ggtagaagtc acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt
     8821 cgttctctgc aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc
     8881 cttcggtatc cgtttctatc tctttacctt cgaagatcag cataattatt actaccttaa
     8941 tcagactggc ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg
     9001 ttagcgggtg agattaatcg cggctggcga agcccagaat gctcagtagg ctgacgaaga
     9061 tgttgtacag cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat
     9121 gaatgatgtt gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc
     9181 tgatcgccag atgcagagca ggcagttgca ggaagatatt agcaatcata ccaatcagta
     9241 ccaccacaat acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca
     9301 gcacatatgc agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta
     9361 cgtcacccat tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa
     9421 aaccggtaaa ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca
     9481 ggaacatcaa accatacata cccaccagag tcagaatcag acccggagat ggcagcatca
     9541 gcaccgtact ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat
     9601 aggtattacg cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa
     9661 caatacgatc cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat
     9721 aatgcgtaag ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt
     9781 aacagcggat tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa
     9841 gaaaaagggg gtagcgttta gctaacccct tatcttattt ggcggaagcg cagagattcg
     9901 aactctggaa ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca
     9961 cacttccgga atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt
    10021 ttgtttgcct gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc
    10081 atttcatcac catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat
    10141 cgcccggcta aagaaaaata ggtccatttt tatcgctaaa agataaatcc acacagtttg
    10201 tattgttttg tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc
    10261 cagtgcagaa ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc
    10321 tacagggacg tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa
    10381 gagacgtgcg atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt
    10441 acccggcgca gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg
    10501 ggaatggcac caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg
    10561 atccacggtc tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg
    10621 gcgaaggacg tcatcctttc cctgatttcc ctcgattacg acgataccct gatggctgcc
    10681 gccggaaccc aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat
    10741 atcctcgcag tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc
    10801 ggtcgaccgt ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc
    10861 tggggaacct gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca
    10921 acgcctatcg acaaagtcat caccgacaaa cccattatca aagtacctgg ctgcccgccg
    10981 atcccggatg tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat
    11041 gtcgacagaa tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat
    11101 cgccgcgctc acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc
    11161 aaaggttatt gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc
    11221 tccacacgct ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc
    11281 tgtgcggaaa atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg
    11341 caaatgggta ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca
    11401 gcagctgttg gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag
    11461 caacctacag aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt
    11521 acgaaactca gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta
    11581 cgcgcatcga aggccacatg cgctgtgaag tgaatattaa cgatcagaat gtgatcacca
    11641 atgccgtctc ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc
    11701 cgcgcgatgc gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc
    11761 tggcttcggt ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata
    11821 tcatccgcaa cattatgctg gcaacgctct ggtgccacga tcatctggtg cacttctatc
    11881 agcttgccgg gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa
    11941 cctccgaact ggcgcaaagt ctctcctctt ggccgaaatc atcccctggc tatttcttcg
    12001 acgtacaaaa tcgcctgaag aaattcgttg aaggcgggca gttggggatc ttccgcaatg
    12061 gctactgggg gcacccgcag tacaaactgc cgccagaagc caacctgatg ggctttgccc
    12121 actatctcga agctctcgat ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg
    12181 gtaaaaaccc gcatccaaac tggattgtcg gtgggatgcc ttgcgccatt aacattgacg
    12241 aaagcggcgc ggtcggggcg gtcaatatgg aacgcctgaa cctggtgcag tcgattatca
    12301 cccgcacggc agacttcatt aacaacgtga tgatccccga cgccttagcc atcggtcagt
    12361 tcaacaaacc gtggagcgaa atcggcacgg gtctttctga taaatgtgtc ctcagctacg
    12421 gcgcattccc ggatattgcc aacgactttg gtgagaaaag tctgctgatg cctggcggcg
    12481 cggtgattaa cggcgacttc aacaatgtgc tgccagtgga tttggttgat ccgcagcagg
    12541 tgcaggagtt tgtcgatcac gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc
    12601 cattcgatgg cattaccgac ccgtggtaca accccggcga tgtcaaaggc agcgatacca
    12661 acattcagca gctgaatgaa caggaacgct actcgtggat caaagcgcca cgctggcgcg
    12721 gtaacgcgat ggaagtgggg ccgctggcac gcacgttaat cgcttatcac aaaggcgatg
    12781 ctgcgaccgt tgagtcggtc gatcgcatga tgtcggcgtt gaacctgccg ctttccggta
    12841 tccagtcaac gttgggccgc attttgtgcc gcgcgcacga agcgcagtgg gccgcaggta
    12901 agttgcagta tttcttcgac aagctgatga ccaacctgaa aaacggcaat ctcgccactg
    12961 cttccactga aaaatgggaa cctgcaacct ggccgacaga gtgccgtggc gtcggcttta
    13021 ccgaagcgcc gcgcggagcg ttaggccact gggccgccat tcgcgatggc aagattgatc
    13081 tctaccagtg cgtggtgccg accacctgga acgccagtcc gcgcgatccc aaagggcaga
    13141 ttggcgctta tgaagcggcg ctgatgaaca ccaaaatggc gatccccgag caaccgctgg
    13201 agatactgcg tactctgcac agctttgatc cgtgcctcgc ctgttcaact catgtgctgg
    13261 gcgacgacgg tagcgagctg atctccgtgc aggtgcgtta acagcgaagg agaatcatca
    13321 tgcaacagaa aagcgacaac gttgtcagcc actatgtctt tgaagcacca gttcgtatct
    13381 ggcactggct gacggtgtta tgcatggcgg tgttgatggt caccggttac tttatcggca
    13441 agccgctacc ttccgtcagt ggcgaggcga cgtatctgtt ctatatgggt tacatcaggt
    13501 taattcactt cagcgccggg atgattttta ccgtggtctt gctgatgcgg atctactggg
    13561 cttttgttgg caatcgttac tcccgcgagc tgtttatcgt gccggtatgg cgtaaaagct
    13621 ggtggcaggg cgtgtggtat gaaatccgct ggtatctgtt tctggcaaaa cgtccgagtg
    13681 ccgatatagg ccataacccc atcgcccagg cggcgatgtt cggctatttc ctgatgtcgg
    13741 tctttatgat catcaccggt tttgcgctgt acagcgaaca cagccagtac gctatttttg
    13801 cgccgttccg ttatgtggtg gaatttttct actggacggg tggcaactca atggacattc
    13861 acagctggca tcggctgggg atgtggctga ttggggcgtt tgtgatcggt catgtctaca
    13921 tggcgctgcg tgaagacatc atgtccgacg acacggtgat ctccaccatg gtcaacggct
    13981 accgtagcca caaatttggc aaaataagta acaaggagcg ttcatgagcg agcaacacgt
    14041 ggtggtcatg gggctgggca acctgctgtg ggccgatgaa ggcttcggcg tgcgggtggc
    14101 ggaacggctg tatgcccatt accactggcc cgagtatgtg gagattgtcg atggcggtac
    14161 tcagggactg aacttgctgg ggtatgtcga aagcgccagc catctgttga ttctcgatgc
    14221 cattgactac gggctggaac ctggaacgct gcgaacctat gccggagaac gcattccggc
    14281 ctatctcagc gcgaagaaaa tgagcctgca tcagaacagt ttctccgaag tgctggcgct
    14341 ggcagatatc cgcggacatc tgccagcaca tattgccctc gtcgggctgc aacccgcgat
    14401 gctcgacgac tacggcggca gcctgagcga actggcacgg gagcaactgc ccgctgcgga
    14461 acaatcggcg ctggcgcagt tggctgcgtg gggaagtgtg ccgcaaccgg ctaatgaatc
    14521 gcgttgtctc aattatgact gtctgtcgat ggaaaattac gaaggcgttc gcttgcgcca
    14581 gtaccggatg acacaggagg agcagggatg agcaacgaca cgccatttga tgcgttgtgg
    14641 caacgaatgc tggcgcgcgg ctggacgccg gtcagtgaat cccgtcttga cgactggctt
    14701 acgcaagcgc cagacggcgt ggtgttatta agcagtgacc cgaaacgcac gccagaggtc
    14761 agcgataatc cggtaatgat tggcgaatta ctgcacgagt ttcccgacta tacatggcag
    14821 gtggcgattg ctgaccttga gcagagcgaa gccatcggcg atcgctttgg cgcctttcgc
    14881 tttcctgcca ctttagtgtt taccggcgga aactatcgcg gcgtgctgaa tggtattcac
    14941 ccgtgggcgg aactgataaa cctgatgcgc gggcttgtcg aaccgcagca ggagcgtgcc
    15001 tcatgagcga aacttttttc catctgctgg ggccaggaac gcaaccgaac gatgacagtt
    15061 tcagcatgaa tccactgccg atcacctgtc aggtgaatga tgaaccgagt atggcggcct
    15121 tggagcaatg tgctcacagc ccgcaggtga ttgcgctgtt aaacgagtta caacatcaac
    15181 taagcgaacg ccagccgccg ttgggcgagg tgctggcagt cgatctgtta aatctcaacg
    15241 ccgacgatcg tcactttatc aatacgcttc tcggggaagg ggaagtgtca gtgcgcattc
    15301 agcaggctga cgacagtaaa agtgaaatac aggaggcgat cttctgcgga ttatggcggg
    15361 tgcgcagacg tcgcggcgaa aagttgctgg aggacaaact ggaggctggc tgcgcgccgc
    15421 tggcgttgtg gcaggcggca acgcaaaatc tcttgccgac agattcgctg ttaccgccgc
    15481 ccattgatgg cctgatgaat ggcctaccgt tggcgcatga gttactggca catgtacgta
    15541 accccgacgc gcagccgcac agcattaatc tgacgcaatt acccatcagc gaggctgatc
    15601 ggctttttct ctcgcgtctc tgtgggccgg gaaatattca gattcgtacc attggctatg
    15661 gcgagagcta tatcaacgcc acggggttac gccatgtctg gcatttacgc tgtacggaca
    15721 ccttaaaagg cccgttactg gaaagttatg aaatctgccc aataccggaa gtggtgctgg
    15781 cagcgccaga agatttggtc gactctgcgc agcggcttag cgaggtatgt cagtggctgg
    15841 cggaagctgc accgacgtaa aaagacggta agtatcgctt tcagtcttat gaatatcgca
    15901 atcggcgaat acctctggtc gtagagtttc aggataaaga gggagatcta acattatcgg
    15961 gttatttttc tctcttcgcc tacaggagtg cgcatgtggg atgtcattga tttatcgcgc
    16021 tggcagtttg ctctgaccgc gctgtatcac tttttatttg taccccttac cctggggctg
    16081 atttttttgc tggctatcat ggaaaccatt tacgtggtta ccggcaaaac aatctaccgc
    16141 gatatgacgc gcttctgggg taagctcttc ggtatcaatt ttgctcttgg cgtggctacc
    16201 ggcctgacca tggagtttca gtttggtact aactggtcat tctattccaa ctatgtgggc
    16261 gatatttttg gcgcaccact ggcgatggaa gcattaatgg ccttcttcct cgaatccacc
    16321 tttgtcgggc tgttcttctt cggctggcaa cggctgaata aataccagca cctgctggtg
    16381 acgtggctgg tggcgttcgg ttcaaatctc tctgcattgt ggatattgaa tgccaacggt
    16441 tggatgcaat acccgaccgg tgcgcatttt gatatcgaca ccctgcgtat ggagatgacc
    16501 agcttcagcg agctggtctt taacccggtc agccaggtga aatttgtgca caccgtcatg
    16561 gcgggctacg tgaccggggc catgtttatt atggcgatca gcgcctggta tttactgcgc
    16621 ggacgggagc gcgatgtcgc attacgctcg tttgccatcg gttccgtctt cggtactctg
    16681 gcgattatcg gtaccctgca actcggagac agttctgcgt atgaagtcgc gcaagtacaa
    16741 ccggtaaaac tggcggcgat ggaaggggag tggcaaacgg aacctgctcc tgcaccgttc
    16801 catgtggttg cctggccgga acaggatcaa gagcgtaacg cctttgccat caaaattccc
    16861 gcgctgctag ggatcctcgc cactcactca ttagataaac ccgtgccggg tctgaagaat
    16921 ttgatggctg aaacctaccc acgcttgcaa cgcggacgta tggcctggct gttaatgcag
    16981 gaaatatctc aaggcaatcg tgagccgcat gtgttgcagg cattccggga gctggaaggc
    17041 gaccttggtt acggcatgtt gctctcccgc tatgcgccgg atatgaatca tgtcacagcc
    17101 gcacagtacc aggcggcgat gcgtggcgcg atacctcagg ttgcgccggt attctggagt
    17161 ttccgcatca tggtgggctg tggttccctg ctgctactgg tgatgctgat tgcgcttgtc
    17221 cagacgctgc gtggcaaaat cgaccagcat cgctgggtgc tgaaaatggc gctctggagt
    17281 ttgccgttgc cgtggattgc gattgaagcc gggtggttta tgaccgagtt tggtcgtcag
    17341 ccgtgggcga tacaggacat cttaccgaca tactccgcgc actccgcttt aaccacagga
    17401 caactggctt tctcactgat catgatcgta gggctttaca ccctgttctt aatcgccgaa
    17461 gtctacctga tgcagaaata tgcccgtctg gggccgagcg cgatgcagag tgaacaaccg
    17521 acgcagcaac aggggtaaag gagaaaatca tgtttgatta tgaaacattg cgcttcatct
    17581 ggtggctgct gattggcgtg atcctggtgg tctttatgat ctccgacgga tttgacatgg
    17641 ggatcggctg tttgctgccg ctggtggcgc gtaatgatga tgaacgccgg atagtgatta
    17701 atagcgttgg tgcgcactgg gaaggcaatc aggtctggtt gatcctcgca ggtggggcat
    17761 tatttgcagc ctggcccaga gtgtatgcag cggcgttttc cggcttttat gtggcgatga
    17821 tcctggtgct gtgctcgctg ttcttccgtc cgctggcctt tgattatcgc ggaaaaattg
    17881 ccgatgcacg ctggcgtaaa atgtgggatg ccggtctggt catcggcagt ctggtgccgc
    17941 cggtagtctt cggtatcgcc ttcggcaact tgttgctcgg cgtgccgttt gccttcacac
    18001 cgcaattacg cgtggagtat ctcggcagct tctggcaact gctgacgcca ttccctttat
    18061 tgtgtggttt gctcagcctt gggatggtga ttttgcaagg tggcgtctgg ttacaactga
    18121 aaactgttgg tgtgattcat ctgcgttcac agctggcgac taaacgcgct gcactgttgg
    18181 tgatgctgtg ctttttgctg gcaggttact ggctgtgggt cggtattgat ggctttgtac
    18241 tgctcgccca ggatgctaac ggtccttccc atccgttaat gaaactggtg gcagtgctac
    18301 ctggtgcctg gatgaataat ttcgtcgaat cgcccgtttt gtggatcttc ccgctgctgg
    18361 ggttcttctg cccattgctg acggtgatgg cgatttatcg tggtcgcccg ggttggggat
    18421 ttttgatggc atcattgatg caatttggcg tgattttcac ggcaggcatc acgctgttcc
    18481 cctttgtcat gccgtcaagt gtgagtccaa tctccagcct gacgttgtgg gacagcactt
    18541 ccagtcagct gacgctgagc attatgttgg taatcgtgct gatatttttg cccattgtgt
    18601 tgctctacac tctctggagc tactacaaaa tgtggggacg catgacaaca gaaactctcc
    18661 gccgtaacaa aaacgagttg tactaaggag caggaacatg tggtatttac tttggttcgt
    18721 cggcattttg ttgatgtgtt cgctctccac ccttgtgttg gtatggctgg acccgcgtct
    18781 gaaaagttaa cgaacgtagg cctgatgcgg cgcattagca tcgcatcagg caatcaataa
    18841 tgttagatat gaaaagcgga aacatatcga tgaaagcgat cttaatccca tttttatctc
    18901 ttctgattcc gttaaccccg caatctgcat tcgctcagag tgagccggag ctgaagctgg
    18961 aaagtatggt gattgtcagt cgtcatggtg tgcgtgctcc aaccaaggcc acgcaactga
    19021 tgcaggatgt caccccagac gcatggccaa cctggccggt aaaactgggt tggctgacac
    19081 cgcgcggtgg tgagctaatc gcctatctcg gacattacca acgccagcgt ctggtagccg
    19141 acggattgct ggcgaaaaag ggctgcccgc agtctgctca ggtcgcgatt attgctgatg
    19201 tcgacgagcg tacccgtaaa acaggcgaag ccttcgccgc cgggctggca cctgactgtg
    19261 caataaccgt acatacccag gcagatacgt ccagtcccga tccgttattt aatcctctaa
    19321 aaactggtgt ttgccaactg gataacgcga acgtgactga cgcgatcctc tgcagggcag
    19381 gagggtcaat tgctgacttt accgggcatc ggcaaacggt gtttcgcgaa ctggaacggg
    19441 tgcttaattt cccgcaatca aacttgtgcc ttaaccgtga gaaacaggac gaaagctgtt
    19501 cattaacgca ggcattacca tcggaactca aggtgagcgc cgacaatgtc tcattaaccg
    19561 gtgcggtaag cctcgcatca atgctgacgg agatatttct cctgcaacag gcacaaggaa
    19621 tgccggagcc ggggtgggga aggatcaccg attcacacca gtggaacacc ttgctaagtt
    19681 tgcataacgc gcaattttat ttactacaac gcacgccgga ggttgcccgc agtcgcgcca
    19741 ccccgttatt ggatttgatc atggcagcgt tgacgcccca tccaccgcaa aaacaggcgt
    19801 atggtgtgac attacccact tcagtgctgt ttattgccgg acacgatact aatctggcaa
    19861 atctcggcgg cgcactggag ctcaactgga cgcttccagg tcagccggat aacacgccgc
    19921 caggtggtga actggtgttt gaacgctggc gtcggctaag cgataacagc cagtggattc
    19981 aggtttcgct ggtcttccag actttacagc agatgcgtga taaaacgccg ctgtcattaa
    20041 atacgccgcc cggagaggtg aaactgaccc tggcaggatg tgaagagcga aatgcgcagg
    20101 gcatgtgttc gttggcaggt tttacgcaaa tcgtgaatga agcacgcata ccggcgtgca
    20161 gtttgtaatg cataaaaaag agcattcagt tacctgaatg ctctgaggct gatgacaaac
    20221 gaagaactgt ctaatgcgta gaccggaaaa ggcgttcacg ccgcatccgg tcactttcag
    20281 ttttactctt tctcggagta actataaccg taatagttat agccgtaact gtaagcggtg
    20341 ctggcgcgtt taatcacacc attgaggatt gcgcctttaa tattaacgcc agcctgttcc
    20401 agacgctgca ttgacaagct cacctctttg gcggtgttca agccaaaacg cgcaaccagc
    20461 aggctggtgc cgacagagcg tcccacgacc gcagcattgc tcaccgccag catcggcggc
    20521 gtatcgacaa tcaccagatc gtaatggtcg ttcgcccatt ccagtaattg acgcatccga
    20581 tcgcgcatca gcagttcaga cgggttaggt ggcacctgac cgcgagtaat cacatcaaag
    20641 tctcctttgc caaaatgctg gatcactttg ttgagctcat ctttacctgc cagatattcc
    20701 gacaagccat gttcattact cacggtaaac aggttatgcg aataaccacg gcgtaagtcg
    20761 gcatcaataa ataacacttt ttgatcggac tgggcgatca ccgctgccag agttgaactg
    20821 acaaacgttt taccactgtc tggcgtcgca ccggtgatca tcagaatgtt attctcagtc
    20881 tccatcatag cgaaatgcag actggttcgt agcgcacgta cggcttccac agcagaatcc
    20941 gccgggttat ccaccgccag gaaggggata tttttagtac gatggcgctg ctgattagaa
    21001 aataaatttt tcttacgcag acgggtgcgt ttatccagcc actcggacat tgggatagtg
    21061 gaataaacgc tgatgccgtg ctcttccagt tgttccgggg cttctacacc acgacgcaac
    21121 atcgcacgcg ccagcacggc actcaccgaa ataaacaggc caagaataaa accaagcacc
    21181 acattcaacg cttttttcgg tttcactggc tgcggctgag tgactgccgg gtcgataatc
    21241 cgcacgttac caatggcact ggatttcgaa atactcaact cctgctggcg gttaagtaat
    21301 tgcagatata ccgcacggcc cgcttctacg tcacgactta aacgcaacac ttcctgttgg
    21361 gtggaaggca ttgccgatac ccgcttattc aggcgtttgc gttcttgctc cagcgtctgg
    21421 cgtttttcca gcagcgcacg ataagttggg tgatctttct tatacagctg ggagatctct
    21481 gcctcgcgga aggtcagctc attgagttga ttatcaacgt tcacaatctg ctcaagaacg
    21541 gctttggctt ccaggttaag gtcaaccgaa tcgcgctgct ggcgataaac gttgagtttt
    21601 tcttccgcct ggtccagctc gctgcgcact tcaggtaact ggcgctgtaa gaattcaagg
    21661 ctttgtgaat cctgcgccgc ctggcgagcg atattctgtt gcaaatagtt gttagcgatg
    21721 ctgttcagaa tacgagtaat caactgggga tcatcaccag tcatggtaag ttccagcatc
    21781 ccgctttctt tactgcgttc gctaacggta aaggtttcct gcaatgcgtt aatcgcttcc
    21841 agttcggtac gctggctcag gacaaactgt gttcctggtt tggccttaat gtccgcgata
    21901 gtcagcgcaa cgccatcttt ttccagacgc tggccgacca taccattgac ggtgaactct
    21961 tcaccttcca gtgtatagtg gccgttttcc ccaaccgtga gtgtcagttg ctgatcctga
    22021 ccattcagtt gtggaatatg catccagctg atcgccagct cacctagttt ttctttggtt
    22081 aatctcgccc agccgcgacc cacaatcgga aaatacttct gctcaactat gtcgcgcaga
    22141 ttcagttcag caatggtttt accgagaatc atgcgcgatt gcagcagttg gatctccggt
    22201 gcagactcgg gcgatgagtt agggatcata tcgctcaggc cgctgagaat ggcgttgccc
    22261 tgtttttgct caacctggac cagagtatct gcctgataaa ttggtgtgct taacagcgat
    22321 cagcgtgaat aacgcggtca cgctgataat aaacttacgg tgatcccata actcgccgac
    22381 cagacgaagc agatcgatct cattttcctg agtgctgcct ggtggcgtat tcatattttt
    22441 agttgtcatc gtattttata aactcaaatt accggctgag gcgcttcgcc cattcctgac
    22501 tggcgcgctc caacatactg tagacatgtt caaatgcgtc ctgactttta cgatagggat
    22561 ccgggatctc tttctgttcc agccattgcc caaacagcat tgtcttcccg cggacctcgg
    22621 gagcaattgc ggtaacctga gcaatatgtt ccgactccat tgccagaatc agatcgtaat
    22681 ttcgtgccat ctcagcagtg agcttacgtc cggcatgtcc ttcaagagat acgccatgat
    22741 tagcggcaac atctgccgct gtcgcatctg cagggtgttt taccagacca tgaacacctg
    22801 ccgatttgac ttttacgccc ggcagacgtt tacgcagcaa gcgctcgcca atgggcgaac
    22861 ggcaaatgtt gccggtacaa accactagga ttgagttaaa ttttagttgg gccatgtttg
    22921 aatccaccgt gaagtctctg tcaggtcatg caccccggaa atcgtcggta ccagcagaga
    22981 aatcacacgg ttccaacgag ccagcggcgc ggtagtgaca taaacaatgt cgtaaggttc
    23041 aagctggaat tccgttccga gtatcattgc cgatgcgtcc ttcgcattca gctgataaat
    23101 attggcgatt ttgccattct gcttgttttg cgttgcacga ataacaaaaa tccccgtcgc
    23161 atcagcgaca tcctgattca taccctcagc gttacccagc gcttctgcca gggtcatacc
    23221 gctgcggtcc attttcagcg tgctctgttt acccacttca cccatcacga agactttcag
    23281 atcatcgttg cgcggaataa ataaaatatc gcccgggtgc agcagtttgt tctggcgcaa
    23341 gtcgccacgc tgcatcagcg catagaggtt cactttggtc tttacgccat tctgggtgag
    23401 caccacgttg cgccagtctg catctgcggt cagaccgccc gcggcgttaa tcgcatccat
    23461 gatggtcaga ggaatattgg taatcggctg ctgacccgat ttgctgactt cgccggtgac
    23521 gtacgctttt tgcgatcgga acgccgcgac gctgacatcg acttgcgggc tttcaatgac
    23581 tgaatcgaga cgcgctgtaa tctcgttgcg tacctgggta agtgttttcc cggccacttt
    23641 caggcgacca atataggggt agaaaatcgc accatcggcg ttaacccagt tgccggtatc
    23701 actggcgcta cggtattgcc ctgcgggggt ggttagttcc gggtgatccc agaccgtaac
    23761 catcaacaca tcaccaatcc cgatacgata ttcatagttt gcgatttgct gctccagctc
    23821 cgggttagcc tgcgacataa tcggtttggc gcgcagctga tcaatcaatc ctggcgtgac
    23881 gggatagata ttgaccattt tgtccaggtc atactgattg tcaggtagtt caatgacatc
    23941 cttgttactg gtcgataaat tctgaccagg aacaagggta catgctgtca aagacagaac
    24001 agccaatgtc agtaccgaaa acttaaaaat gttctttttc attggtttat atttatcagt
    24061 caatgtaatg ttatgaatta cgtcaggtta ttgtccaacc ggtacttctc gctctgccgt
    24121 cattggatcg agctggtatt tgcgtgacag catttgtcca ccatcacgcg tcaatggtgt
    24181 ccacgaaacc accgcgcggt tgcggttagg tccaatggtc atcaagtcga acggaatcga
    24241 gatataaaaa cctttactaa agccgccttc gccgtagtca tctttagata cgttactgat
    24301 tgccgcccat acccctaccg ccacgccgct gtcaaagagt ttggcgacgt cgatcgttgc
    24361 gcctttatct tttgccagat attgcccaac gctaagtttc atcagtacgc cattgagcgt
    24421 cggcgggttc cagtaagccg tcacgaaacc tgtgggcgtg gaataatcgg tgaagcgcat
    24481 catgttgtcc cagtcacgct gcttcacgta gttaacgtcc acacccagcg cccagctggc
    24541 atctagcggg cgataaagaa gctcggaacc gacaccggca tacatcgttt ccagataacc
    24601 gccatacacc tggccataga aaccattgcc taagtcggca aagtaattcg cttgcaagtt
    24661 gttgagataa acgtcattgc gaacgtaatc acggatatgc gtgcgcacgc ggggcagggt
    24721 agagtccgcg ggtaacagtg aagacttaaa cttgtcgtag ttgttgtaaa tattggtgaa
    24781 aataccgtca tcaagcagca ggtggtcggt aaaccagtag cgggcgctgg acatcagccc
    24841 cagctggaac atatagaaat cttccgggcc gcccagcgac tgtgaaagtg ttgggttgaa
    24901 agagtagcta aagcgatctt cacgaatacg gtagcaccga ccaaaggcag aaagatcttc
    24961 tgcttcaaca cgttgttgtt gcagttgctc tgatttacca ggcgctgtgc cggccagctg
    25021 tttgcgcagg ctggctacgt cggtttcggt agtcaccatc gccatatgct cgcgcttttg
    25081 cgtcacgcta atcttctcaa cgccttgcgg caggttattc accagaatcc ggttggcacg
    25141 atcgaccgct tcgcgagagt cacggtattt gtattgctga ccagacatat acagcgtctt
    25201 atcgcgtagc tgaatttctg gcgcgtcaaa gcccgcgtta tacttcaggg cggtaagttg
    25261 gtttgctacc gtggtgtact gcaaaccttc agactctggc gcaggctgcc aggccggttt
    25321 tggcgtatcg cgtagggcag ggcgcagatc gttgaaattg gtccgtaaag tgaaaccaaa
    25381 catcaacgtg tttccgcgtt cataactgag gttgaggtct gcccagctgg cagcgcgata
    25441 aactgcaccg acgttgaaat ggcttgcctg aggcagtttg ccagcgaaat cattctggta
    25501 attgttgccg tcgtattcga gtttcagacg caggggattc cacggcgttt gatactcaat
    25561 gccgccaaag atggaagccg ggccacgaaa gatatcgcta aagctgatat cgcccgcatc
    25621 gtgagactct gcgcgatgac aatatttatc gctcacccgg caaaacgggt tggtaatatt
    25681 gcccgcatta ccggcgtagc cccatgccat cccgagggtg aaatcaaatg gccccgcttg
    25741 cttgctggcc accagatatt caccgtcaaa caaacccgta ccagcaatat cacgtttacc
    25801 aaacgccact tgcggtagcc agtaaccttc ttcccacagg cgaagtttaa aatcgaatga
    25861 tttgtctttg tatgactgat cgccgctgaa atcttcccac tggctatatt tgcgtgtgcg
    25921 cacatccgta taacgaatgg tgccttccag ccatgggaac agcgccacgg aggtggagta
    25981 gaaccggtat tgatcgttat cccggtagtt gacgctgaat tcaccttccg gtgcgatgcg
    26041 cgcatttggc atctgcaaca atcctgtgcc gccaaagtct gattgcgacg aacccagcgg
    26101 atccgggtag gttaatactt cagcatgaca ggcactggag acggcaatgg ccaggcagct
    26161 taaaagataa gaattcttct tcatcagtca ggaacacgtt gcgttaaaac agacacaatc
    26221 tggtcattta aatcagcata tttttcgggc agtacatgcg cagaaaagcc caaccaaagc
    26281 tggctgccag gcggtggttc cacatgtctt ttattccaca gggcaacagg ggcgacgaca
    26341 gtctcaccct caggcgtaat caccataacg ttattcttgt ctgcgcctgc aaggcggggg
    26401 tgatcttgca gataatcggt aacgctacgg cctgcctgcc agggcaattg cccagtccca
    26461 gaaaccgcac ccagcagagt aatagtcacc ggacgctgta cggtatagag cgtatagtcc
    26521 ccgaccaacg ggggattgct gttctcatcc actcgtacaa aatcagggtc cagtttgacc
    26581 ggtaagcgac cagtgatatt gagattgagt aactgctggc gcacggattt aatcgttgcc
    26641 gccacatcgt cgtcagcttc tgcttcccag gaagcaagct gtgccatgac atgttggtag
    26701 tctttaagcg ctttagcttt cgccgcagag tcggtcagca aggcaccagg ccaccagagg
    26761 cgatcgcgca gttgcggttg tgtcaccaac tggacaacat tttccacggg gccaacggaa
    26821 agtgtctgtt gttcgctagg cagataaata gtcaccgttc cttgcgcaaa ggcatggggt
    26881 gtcattacgt aaagtacgct ggcaatgaaa tacgactgta atttattcat ggtgcgggtt
    26941 taagaaacgt catttctacg ggaataaccc ctgcgcctaa catttgccga ctttgacgaa
    27001 cctggccagt agccgaatcg acccagaatg tgttttgcca ctgtttttct ggcagcgtgg
    27061 aagtcacctc ttcctgccag acattacaca gcacattgtg acccgcaata ttcagcgtct
    27121 ctaatccagc aaaggaaaaa gtagaactta gggtcgcaga gcgaaaatgg ttgtcttcac
    27181 tccagagaat atcgcgcgtc cagcggctgc catcttttat tgccagcgcg ttacgcagag
    27241 gggcccgccc gggatttgtc acttccagca gattattatt aagtttgacg gtctttaaaa
    27301 gacgtccatt gtgggtaacc agcatggcgt tatcctggga taaccatttg ctttgttctt
    27361 gttctatata tcccagtacc acaaagattc gttgcccttc attaaggcgt aaatacatcg
    27421 tggaataagg caacgcctgg atctgctgag ccgctacagt gatatcctga ttatcgaaaa
    27481 ggctggcgcg aaatgtatcg accatacttt gctggctgtg cgtacatccc gcaagaaata
    27541 gtgcgaaaat cgataaaata agagggcgca cctttaatcc ttgaaaggtt aattaaaaaa
    27601 atgcaaatct ctaaagcccg gtacgaatat cgcaaccggg tattatttaa cttgaatttc
    27661 gacgcgttta agcagaatta gcgcgtagtg gacgtggtgg ttgaggtgtt atgagaaccg
    27721 ccatcgccac tgtcatttgc tgcaactact gccaacgtcg ccacaccaac agccgttgcc
    27781 acaccgacaa ctgtcgccgt gccgacagcg ccagcagcca cgccactagt ggtagccgca
    27841 gtcgtggttg cacctgtagt ggtcgttgtg gtgccagtag tacccgtcgt tgcctcggtc
    27901 ccgtttgctg cttcaggggc agcaaacgca gctggcgtcg tcaacatgaa ggccataagg
    27961 attgcagaaa gcttgtgttt cataactttt cctttattca tcgcatggac aatacgtacg
    28021 cttgagatca ggattgtcta ctttgagtat aacatccttg tgaaatctcc ctttcctcat
    28081 accgctagca ctatatactg cgggtgggaa aggcgcgcag agggaaatat aagattgtta
    28141 acgatttccc tctggaaata cgacttgtga gcataagata aatcttagaa aaatacgaat
    28201 taaagataag aaaattgtta cgaaagctat taattattgt tggtaatata gtttcaagtg
    28261 gtactataac tggctgttaa atggcattta taactattag gtgcagagat attcgcttaa
    28321 atggattagt ttcttagtgg aaaagtaact gatgttataa tgtatggtgt tgactattga
    28381 actattccaa taaaaccagt cagctttaaa caagcagcgt catattaaga gagataaaca
    28441 tttgccgctg ttggtcctcg caggccattt acgcggcaaa attccacgcg taatcctggt
    28501 ataagcactt ctgcgtcgcg gggagtgaat gcggaaatat ggacctggac ttctttacga
    28561 ccgtcggagg ggataatgaa tcctttgccg cttttgcgat caaaggtttt gacaattcct
    28621 gtcattttac gggacaaaca aattccttac tgaaaatact gcgctgcact atacggggtt
    28681 aataaaataa agccagcgat atttaagacc gccggacggc taaaataaaa tttgcttaat
    28741 ctcaattatc atgccttaat agctgcgtcg gtttgaaaga cagacagcat acaaagtagt
    28801 ttactaaagc agttctcatt atcaggcatt atccccttct tttgagtctc tctcctgaac
    28861 actaagtggt ttctgtatta aagccctgtt tgccgaaagg cccaaaatga aggaagtaaa
    28921 atatgtctaa ta
//
LOCUS       DAJTRH010000047        28419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTRH010000047 DAJTRH010000000
VERSION     DAJTRH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..651
                     /locus_tag="OO339_002195"
                     /pseudo
     CDS             1..651
                     /locus_tag="OO339_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            648..1034
                     /locus_tag="OO339_002196"
     CDS             648..1034
                     /locus_tag="OO339_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926815.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1059..3272
                     /gene="tssI"
                     /locus_tag="OO339_002197"
     CDS             1059..3272
                     /gene="tssI"
                     /locus_tag="OO339_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5926816.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3295..3740
                     /locus_tag="OO339_002198"
                     /pseudo
     CDS             3295..3740
                     /locus_tag="OO339_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3762..4169
                     /locus_tag="OO339_002199"
     CDS             3762..4169
                     /locus_tag="OO339_002199"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5926817.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4166..6454
                     /locus_tag="OO339_002200"
     CDS             4166..6454
                     /locus_tag="OO339_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5926818.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6451..7440
                     /locus_tag="OO339_002201"
     CDS             6451..7440
                     /locus_tag="OO339_002201"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926819.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7540..7743
                     /locus_tag="OO339_002202"
     CDS             7540..7743
                     /locus_tag="OO339_002202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926820.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7761..8081
                     /locus_tag="OO339_002203"
     CDS             7761..8081
                     /locus_tag="OO339_002203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926821.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8147..8379)
                     /locus_tag="OO339_002204"
                     /pseudo
     CDS             complement(8147..8379)
                     /locus_tag="OO339_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8489..9259)
                     /gene="yafV"
                     /locus_tag="OO339_002205"
     CDS             complement(8489..9259)
                     /gene="yafV"
                     /locus_tag="OO339_002205"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5926822.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9440..9886
                     /gene="ivy"
                     /locus_tag="OO339_002206"
     CDS             9440..9886
                     /gene="ivy"
                     /locus_tag="OO339_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5926823.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9929..12373)
                     /gene="fadE"
                     /locus_tag="OO339_002207"
     CDS             complement(9929..12373)
                     /gene="fadE"
                     /locus_tag="OO339_002207"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5926824.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12613..13191
                     /gene="lpcA"
                     /locus_tag="OO339_002208"
     CDS             12613..13191
                     /gene="lpcA"
                     /locus_tag="OO339_002208"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5926825.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13397..14164
                     /locus_tag="OO339_002209"
     CDS             13397..14164
                     /locus_tag="OO339_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5926826.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14135..14875)
                     /locus_tag="OO339_002210"
     CDS             complement(14135..14875)
                     /locus_tag="OO339_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5926827.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15259..15780
                     /locus_tag="OO339_002211"
                     /pseudo
     CDS             15259..15780
                     /locus_tag="OO339_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15956..16453
                     /gene="rayT"
                     /locus_tag="OO339_002212"
     CDS             15956..16453
                     /gene="rayT"
                     /locus_tag="OO339_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5926828.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16670..18382)
                     /locus_tag="OO339_002213"
                     /pseudo
     CDS             complement(16670..18382)
                     /locus_tag="OO339_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18354..19139
                     /gene="lafU"
                     /locus_tag="OO339_002214"
     CDS             18354..19139
                     /gene="lafU"
                     /locus_tag="OO339_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5926829.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19210..20265
                     /gene="dinB"
                     /locus_tag="OO339_002215"
     CDS             19210..20265
                     /gene="dinB"
                     /locus_tag="OO339_002215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5926830.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20317..20610
                     /gene="yafN"
                     /locus_tag="OO339_002216"
     CDS             20317..20610
                     /gene="yafN"
                     /locus_tag="OO339_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5926831.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20613..21011
                     /gene="yafO"
                     /locus_tag="OO339_002217"
     CDS             20613..21011
                     /gene="yafO"
                     /locus_tag="OO339_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5926832.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21021..21473
                     /locus_tag="OO339_002218"
     CDS             21021..21473
                     /locus_tag="OO339_002218"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5926833.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21719..22762
                     /locus_tag="OO339_002219"
     CDS             21719..22762
                     /locus_tag="OO339_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5926834.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22824..23438
                     /locus_tag="OO339_002220"
                     /pseudo
     CDS             22824..23438
                     /locus_tag="OO339_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23495..24952)
                     /gene="pepD"
                     /locus_tag="OO339_002221"
     CDS             complement(23495..24952)
                     /gene="pepD"
                     /locus_tag="OO339_002221"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5926835.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25214..25672
                     /gene="gpt"
                     /locus_tag="OO339_002222"
     CDS             25214..25672
                     /gene="gpt"
                     /locus_tag="OO339_002222"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5926836.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTICISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25764..27008
                     /gene="frsA"
                     /locus_tag="OO339_002223"
     CDS             25764..27008
                     /gene="frsA"
                     /locus_tag="OO339_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5926837.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27066..27467
                     /gene="crl"
                     /locus_tag="OO339_002224"
     CDS             27066..27467
                     /gene="crl"
                     /locus_tag="OO339_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5926838.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27506..>28419)
                     /gene="phoE"
                     /locus_tag="OO339_002225"
     CDS             complement(27506..>28419)
                     /gene="phoE"
                     /locus_tag="OO339_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5926839.1"
                     /translation="SKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQ
                     QKTRLAFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASG
                     LATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAY
                     TNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQ
                     NFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDY
                     KINQLDSDNKLNINNDDTVAVGMTYQF"
BASE COUNT         7011 a         7071 c         7565 g         6772 t
ORIGIN      
        1 aaaacaaata ttgatggcaa tatcattata cataaggatg gtaggaggac gtatttatta
       61 aatcaaaata ctggttcgat tacggctgtg ataagatgga agtatgagta gtataaggat
      121 aataccgtta cagatgactg gactatatgg gaaaaagcta tttttatgcg agcgatatat
      181 ttatctgttc agcaggcatg ggatgggaaa ataacttatt ctgtttccgg tgaatctgag
      241 ttcgctaaaa aatttcaggg taaagctttg ccttttgatg taagaataat ttctgcatct
      301 cagaatgaag actggttggt tattgcaaca aaggttttgc ctggtgctga tttaagaaca
      361 tatgttgatt ttaaaaacag tacggttcat gttgattcag cgggcctgga aaaagtagcg
      421 aaatgtatta actgtaataa tacgttgcaa gtaaatatac ctcatgaagc cggacatgta
      481 cttggttact tagatgatga ttatgattct tcgtctccgt atgttggaga tattagtggg
      541 ttgatgaatg tgggaatgga gctttgggaa cgatacctta aaaacgcaac tataacgttg
      601 aatatcataa tgcctgaaac taaatttact ttattgaatg taaccaaatg aaatttttct
      661 gtattatagt gattttcctt tcaataaatt acatagctat agtgaatgtt ttcaagtctt
      721 ctgcagttgc tcaatccacc aaatattatg aagaaaatga tatacatgat ggaagtaatg
      781 acatcttttt tatgaagaaa tatgggccgc ctgctcatga gttcgtgagg ccacgatata
      841 aatttgttgg tgagtattat atcggcttga atcaaactta tcgcataaca gatcctgaat
      901 ttcaagatgt tcctattaag gaaatgttct ggaatttgca gggggatttg aatctgacat
      961 gttggtttca ttataaagac gggcaatgga gagtgattac ttatattttt tggccaccag
     1021 gggctagatt ttaatccggt attaaaggag caaatatcat gagttttgta tctacgggta
     1081 ataaaccagt tggaaacggt ggacccgtca tcaccacacc accaatagct ggcgaaagtg
     1141 gcggtatgag cacaggttca gctgtaaccg atgtatctgg tgccgccgag gagatggcag
     1201 aacaggctgc ggcggacctg tttggtgcgc tgccggaacc gtcaggtctg gttaaagccg
     1261 cagtagctgc tgcgcaggct gccgctgcag cagcagggat ctcggatatg gctggtgcag
     1321 ttcaggatgc agcggcgtcg cttgcagccg gtgctccggg ggcgcataac gtcacagtaa
     1381 gtggtagtgc ggtgccgccg cagatgcttt tgtttgcagg catgaatggt tcggaagggc
     1441 ttgggaatct gttttcttac acagtacagc ttaaaacgct ggacgccctg aacctggggt
     1501 acgtttcccc cgctgctaac ctgccgcttc agctcatggt gggcaaagac ctgtgcgtca
     1561 gtatcgaact ggacggcggc ggaaaacggt atatcagcgg gctggtgacg gcggcgaggg
     1621 tggtcggcca tgagagccgt tcggtaacgt atgaattacg cattgagcca tggctgaagc
     1681 tgctgaccca taccagcgac tacaaagcgt tccagaacaa aaccgtggtg gatattctcg
     1741 atgaagtact ggatgaatat tcctttccgg tggaaaagcg gctggtggaa aattatccac
     1801 cccgcgcctg gcaggtgcag tatggtgaaa ccgacttcga tttcatccag cgcctgatgc
     1861 aggaatgggg gatttactgg tggtttgagc acagcgagaa cagccacacg ctggtgctgg
     1921 tggatgccat caatggtcat aaagcgtgcc ctgactcgcc gctggtggag tggcaccagg
     1981 aagggctgaa actggataag gaatttatcc acaccattac ggccagtgag aggctgcgta
     2041 ctggaaagtg ggtgatggac gattttgatt ttatgaagcc tcgttcgctg cttaaaagta
     2101 ccgtggccag tccgcgtgat accggtcatg ctgaatacga gcattacgaa tggcctggtg
     2161 attacttcac tacgggcgaa ggcgagatgc tgacgcgcat tcgtatggaa gcgcagcgca
     2221 gcccaggcag ccgggcacat ggggccggtc atatccgtac cctcatgacc gggtacacct
     2281 ttacgttgat gaatcacccg acggcagaaa ttaatcagga atatctgctg gtacagacca
     2341 ctctgttttt acgggataat gcgcagcaca gcggtcagga tcagcatttt acctatgtca
     2401 ccacgtttga actgcacccc accagcgaag tttaccgccc acaacgaacc ctgagcaaac
     2461 cccacaccaa aggcccgcag agcgccatcg tcaccggccc ggcggggcag gaaatctgga
     2521 cggataaata tggccgggtg aaggtgcagt ttggctggga ccgttacgga aagaatgacg
     2581 aaaacagttc ctgctggatc cgcgtcagct atccgtgggc gggtaaagga ttcggcatga
     2641 tccagatccc gcgtattggc caggaagtac tggtggattt caaaaacggc gatccggatt
     2701 tgcccatcat cgtggggcgc acctataacc aggacaccat gccgccgtgg gggctgccgg
     2761 gcatggcgtc gcagagtggg atattcagcc actcattgca gggagggccg actaacggaa
     2821 atatgctgcg cttcgatgat aaaacgggtg cggaagaggt gaagttccat gcggaaaaag
     2881 acctcaatac cacggtaaaa aacaatgaaa cccacacggt catggtcgat cgcataaaaa
     2941 ccattgtcaa aaatgaaacc atcagcgtgg gagaagaccg taatgccacc atcacaaaaa
     3001 atgatggtct ttccgtcaaa ctggctcaaa ccatcaatgt cggaacgaca tatcgtctgg
     3061 atgttggcga tcaatttacc cttcgttgcg gcaatgcagc gcttgtatta cacaaggatg
     3121 gctcgattga gttttgcgga aaacaactgt tactgcacac cagcgatgtt atgcaactca
     3181 ttggcagtgg cattgatatg aatccggatg gcggcacagc catgactgcg gatgatattg
     3241 ccccgttacc tacccccgac gtttctgaat aaaaaatata aaaggaattt ttctatgacc
     3301 agcctttacc gtattcagga gggctgcttt gccctgcctg aaacttttct tgatcgcacc
     3361 gtcaatatct tcgtcccatc gggcaatgaa agggcaactc ccagtctgaa tattttccgc
     3421 gatacgctca ggccagatga aaatctgacc acctatattg accgccagat agccttgatg
     3481 aaaaaaaaaa cctaggccag caccgggtac tttcgcgggg aacggcactg gcgggaactg
     3541 gcgaagctgc catgacaggt gaacagattc tcgcaactca tcgttctgga aaaacagaag
     3601 tgtaccagcg tcaggccggg tttatcaccg ggccagcaaa ggtactaatg ttgacgctga
     3661 ccactcagcg tccttttgat gatcacaccg accagctatg gactgcatgg ttgactagtt
     3721 ttcagcctgc taaaagctaa aaaaaacagg gaggttttgt catgtatgaa gccgcacggg
     3781 tggatgaccc catctaccac accagcgccc ttgccgggtt tttgataggg gccatcatag
     3841 gtatcgccat tattgccgtc gccagctttg ctttttttac ctgcggcttt ttggccgggc
     3901 tgattttgag ttttctggct gatcagatag cttccggagt cctgcaactg ggtgaggcta
     3961 ttgggcgctc gatacattcc acagtgggaa aaattctgac cggttctgat aatgtcagca
     4021 ccaacagtcg cccggccgcc cgggccgtac tgagtacggt gatatgcgaa gaccattccc
     4081 cggaaatccg cattgcccaa ggttccggca acatctacat caacagccag ctgcccgtaa
     4141 ggatgaccac actgagtgtg atgcagtgat tgaggacggc tcccccaatg tctttctcgg
     4201 cggcggtacg cagacggtac tggaaatcag cccggaaatc cctgactggc tgcgtcaggt
     4261 ggtggatgtg ctgtatgtgg tcgccggaat gcttggcggg ctggctggcg cctggcgtca
     4321 ggcggcaaag atggggtcga aatttggtac aaaatgtgcg gcaaaattta ttggtggcga
     4381 aatggtcggg atggccatct ctgacacagt catgggattg ttcagcaatc cggtagatgt
     4441 caccactggg caaaaaattt tgctgccgga gacggatttt accctgccag gacgcctgcc
     4501 tgtcacctgt tcgcgctttt acgccagcca cctggaaact gaaggattgc tgggcagagg
     4561 atggcggctg aactgggaga taaacctgcg tgaggatgaa acatatatca ccttcatcgg
     4621 tgtacagggt cgggaactga gttatccgaa agagatgctg ataccgggcc accagatatt
     4681 cgacccggaa gagcagtttt acctgagccg tctgcatgac ggatgctatg tgctgcatta
     4741 caccgactgc agctattacg tgtttgatga atttgatgac cacggcgtcg cgccgttact
     4801 ttttatggag acgccgtacc ggcagcgtat tgctttcggg cgtgagaacg gcaggctggt
     4861 gcgggtggcc tccagcagcg gacaccatct gttattgcac cgcacgatga cgcaagcagg
     4921 ggaacgcctg tcgcatattg aacttttgaa gggcgggcgt cccggtaacc tggtggaata
     4981 tcgttacgac gataacgggc aactgactgg cgtggtgaac cgggctggtg tgacggtgcg
     5041 ccagtttgcc tatgaaaatg gcctgatgac agagcatcgc aacgcgacgg gctttacctg
     5101 tacctaccac tgggaagaga ttgaaggttt cccgcgcgtg gtggaacaca cgaccagcga
     5161 cggcgaggat taccggttcc actatgattt tgctggcggg cagacggtgg taaccggcag
     5221 gccggagcag aagtggcagt ggtggtttga tgaagagacg tatgtgactg cgcaccggac
     5281 gccgggcggc ggaatgtacc gcttcacgta caacgaaaac cattttccgg tagcggtgga
     5341 actccccggc gaacgcaggg taacgctgga atacgacacc ctgagccggg tggtgaaaga
     5401 gacggacccg gcaggccggg tgacacagac gcagtggaac ggcagttttg ctgaaatcac
     5461 gcgccgggcg ctggatgacg accacgtatg gaaggcggat tacaacgaac acggccaggt
     5521 gatacgggag acggacccgg aagggcgcgt cacgcggtac gggtatgatg atcaggggct
     5581 gccggagacg gtctgccacc ccggtaaaca gcaggaccgt tacacctgga acgcgctggg
     5641 gttactgagc agccaccgtc gcatcacggg cagtgtgcag tcctggcagt atacgcagcg
     5701 cgggatgctg gcgcgccata cggatgaaga gaaacgggag acgcgctggc agtacacccc
     5761 ggaagggctg gttgccagtc tgagcaacgg caacggggct cagtaccggt tcagctacga
     5821 cggcgacgga cggctgacgg gagaacagcg cccggacggc ctgataagga tgtttgcgct
     5881 gaatgcggac gggtttccgg tcatcatccc gacgcagggc acagagggcg gggtgcgcaa
     5941 tgaacagcag gagcgtgatg cgctgggccg cctgctgcgc agtgacacgc agcacagtac
     6001 acggaccttc agctacaacc gtcttgacca gataacggaa gtgacgctca cccccacgga
     6061 agaaggggaa cggctgcacc acatgcaggc ggacacggtg cgttttgcgt atgaccgcag
     6121 cgggtggctg acggcagagc acagcgtgca cggcagtatc aaataccggc gcgatgcgct
     6181 gggcaatccc accgatatca cgttgccgga cgggcagcat ctcagtcatc tttactacgg
     6241 cagcgggcat ctgctccaga cggcactgga cggtatcacg gtcagtgaat atgagcgtga
     6301 cagcctgcac cgtcaggtga tacgcacgca ggggaaactg gcgacgttca gcggctacaa
     6361 cgccgacaac cggttaagct ggcagcgcag cctgccaggt ggcagccaga atccacagca
     6421 ggcggtactc ccccggcgca gaacgacagc gtgacggcgc gggactatat ctggaacgca
     6481 gacggtgaag tcggcggcat caatgacaaa ctgcgcggtt gcctggtgtt cagctatgac
     6541 cgcagcggct ggctgaccag ccgcacgggg cagatgtatg accatgacca ttattattac
     6601 gacaaagcgg gcaacctgct gacggatgag tatcagggag cggtgatgga taaccgcctg
     6661 ccgggttacg ggcgtgaccg ctaccgctat aacgaatggg gcgaactgac agagcgcagg
     6721 gaccagcagc tggagtggaa cgcgcagggc cagttaacgc gggtcatcag cagcaacagc
     6781 gagacgcggt atcagtacga tgcgctgggc aggcgcatca gcaaggcgac cagcaatctc
     6841 cataccgacc ggggagaacg aagccggaca acgtttgtgt gggaagggtt ccggctgtta
     6901 caggagacaa cgtggcaggg aaagcggaca tacctgtacg atgcggaaca gccgtacacg
     6961 ccggtggctg ccatcacggg gcgcggagag agccagaaaa tctggtatta ccacacggac
     7021 ctgacgggca cggtacacga ggtaaccgcg ccggacggga cgctggtgtg ggcgggctac
     7081 caggcgggat ttggtgaaaa ccggggcgac atcagcaaca gcggggcgta ctttgagcag
     7141 ccgcagcggc tgccggggca gtactttgac gaagaaacag ggctgcatta caatctgttc
     7201 aggtattatg cgccggagtg cgggaggttc gtaagccagg acccgatagg attaaacggt
     7261 gggttgaact tatatgctta tgcgcctaat ccgttaggtt ggatcgatcc gctaggactg
     7321 aattctttag gaatagattc aaataatata tttagaggag atggtaacta taaaggtggc
     7381 ggaattggac agccaaagag tggcacaata tcagcacagg acattattga tcatctctaa
     7441 gaaaaaggtg atgggcgttt aacatctttt agtactaaaa aaacatctag ttccaactct
     7501 cgtggtgcag attttttctc aaaaaaacat gtcttcaaag tggcagtttc tgatctcgat
     7561 gatttagtta aaaataatca gatcactgtt ttaacacaag aaaaagtaaa agcaatattt
     7621 ggtttagaat caagcaagaa aattagaaaa gaacttggta atattcttca gaatatggct
     7681 aggaataatg aggtgttagt tcatgggcaa attcttggaa ataaagttag ccgatgtaat
     7741 tagttagtgg agaggaagtt gtggacgaaa ttagaataag aattgctggt aatacggctg
     7801 cgccttgttt ccatgtgcta aaaaataagg gatataaagt aacaatggaa actcatgcat
     7861 tatctgacga ttgtttagat tgtgtatatg attggtatgc ggaaaaaaat ggacaatttt
     7921 ttagtgcaac gagtccagaa gagttacttg gattagttgc aatgtgggaa gttcgtggta
     7981 atgactggag agctaatttg aatgagataa atacttatca aaaagccata gaagaatccc
     8041 cgttttttga taaaaacggc aatgaaatag agtctgacta aattttattg atatagcaac
     8101 gataataacc ggaaatttct tccagatctt tccggctttt tattatttaa ctttcgttca
     8161 gcacactaaa catcccgtct cgcatcccct taactggctg tttaacctga taaagttccc
     8221 tgcgcagttc ctgtacttcg ttgttatccc aagcaggatc aaacatccct ccgagatctt
     8281 cacggtaacg cccgtgccga tctcaaatcc cgcttttctc agccatttgg cgctgatttt
     8341 gatcaccggc gtgtcgctgc ggttgggcag ataacccacg gtacagcgac gctgtgtttt
     8401 ggaaatggtg acttctggcg taatattgtg tttaagttag gggctttcgt aatctagccc
     8461 agcccccggc cctaaacaca acccccactc accacaacct aaactcatcc gcatcccgcc
     8521 atgccggaaa cttttctcta tattcccgca atgccaccat cgacagctcc gcatcaatac
     8581 gcgttgcctt atgcgcgtcg gcggtagcga taatctcgcc ttgcggatta atcacccggc
     8641 tgtcaccgcg ataatggcag ccgttgccat cgctgccgac gcgattgcat cccgccacat
     8701 acgcctgatt ctcaatcgcg cgggccgtca gcaatgcctg ccagtgcaga gagcgcggag
     8761 ctggccagtt ggcgacgtac agggcgaggt cataatcgtt gagattgcgc gaccacactg
     8821 gaaaacgtaa gtcgtagcac accagcggca aaatacgcca gccgcgccat tccacaatca
     8881 ctcgcgcatt gcccgcttta taatgcagat gctcatctgc catgcggaac agatgacgct
     8941 tatcataaaa atgtaccgtg ccgcccggct caaccagcaa aaagcggtta accgaaccag
     9001 actccgtttg taatgcaaca ctgcctgcaa tcagcgcatt gcactgctgc gccttagctg
     9061 tcatccagtt cactacgtca tcttgtgcaa gcgacgaagc tgccgcttcc atggcaaagc
     9121 cgctggtaaa catctccggt agaacgatca catcgcgccc ggtaatacct tccagttgac
     9181 gatcaaaatg gcgcaggttg gcaggaccat ccacccacac caacggttgc tgcaaaagcg
     9241 taatcttcaa actaggcacg gtgtacaact cctttatgcg aagggtttta taactttaac
     9301 accttatcag gcagttgcct tagcgcagaa taaattgata acaaatgctg atattggaaa
     9361 tatctgattt gcaaattatc gtgttatcgc taggctttag gaggttaata acatgggcag
     9421 gataagctcg ggaggaatga tgtttaaggc aataacgaca gtcgccgcac tggtcatcgc
     9481 caccagtgca atggcgcagg atgatttaac cattagcagc cttgcaaagg gcgaaaccac
     9541 caaagctgcg tttaatcaga tggtgcaagg gcataagcta cctgcatggg tgatgaaagg
     9601 cggtacttat actcccgcac aaaccgtaac gttgggagat gagacgtatc aggtgatgag
     9661 cgcgtgcaaa ccgcatgact gtggctcgca acgtatcgct gtgatgtggt ccgagaaatc
     9721 taatcagatg acggggctgt tctcggctat tgatgagaaa acgtcgcaag agaaactcac
     9781 ctggctgaat gtgaacgatg cgctttcgat tgatggtaaa acggtgctgt tcgcggcgtt
     9841 gaccggcagc ctggaaaacc atccggatgg ctttaatttt aaataattag cggataaaga
     9901 aacggagcct ttcggctccg ttattcaatt acgcggcttc aactttccgc actttctccg
     9961 gcaactttac cggcttcgtc gccagctctt ccggatcaaa gtcatcaacg ttaatactgc
    10021 gcagacggct ttcttccgct ttcaccagaa tagcggcttc atctttatca atcagcccct
    10081 tcgtcagcgc gttgtgcgcc agttcatcca gacgggtaaa tggcagattt ttacccagct
    10141 ctttacagat ccgctgatga atcgggtcgg cggcaatcac atccaccaac gcctcttcca
    10201 gtaaaccaac cgggttatgc tcgcttggcg tcaggtactg accgcgacca atacgtgaac
    10261 gagtggcgtt cggcacttgt aaaatcttcg ccattttatg atccagcttg tcagacggtg
    10321 ccagataatg acgtccggtc gggaagatca ccacattcag cagcccggca accacgcggt
    10381 tcgggaagtt ttgcagtaaa ttgtccattg cctgttctgc ctgatacagc gcatcctgta
    10441 cgccccagtg caccagcggc agatcggctt cattacggcc ttcgtcgtca taacgcttca
    10501 gaacggcaga ggcgaggtaa agctggctta aaatatcccc cagacgtgcc gagatacgct
    10561 cacgacgttt caggctgccg cccagcagtg ccatcgagac atcagaaagc agggcgaggt
    10621 tggcgctcag gcggttcagg tgctgatagt agcgtttagt ggcatcgccg gttggcgtgc
    10681 tgctggttaa accgcgcgtc aggcccagcc agaagctgcg aactttgttg ctaccgacgt
    10741 gaccgatatg tttgaacaac agtttatcga acgcgttgac gtcattgttc ttcgccgctt
    10801 ccatctcttc cagcacgtac ggatggcaac gaatcgctcc ttgtccgaag atcatcatgc
    10861 tgcgggtcag aatgttagcc ccttcaacgg tgatggcaat cggtgcgccc tggtaagcac
    10921 gcgccaggaa gttgctttgc ccgagcataa tgcctttacc gccggtaata tccatcgcat
    10981 caataatcga ctgctgcccg cggtgggtac agtgatactt aacgatagcc gacagcacgg
    11041 caggtttttc gccgagcata atgccgtagg taatcagcga tgccgcagca tccattacat
    11101 aagcgttccc ggcaatacgc gccagcggct cttcaatccc ttccatctta ccgatagaga
    11161 ttttgaactg acggcgaatg tgagaatacg cgcccgttgc cagcgctacc gatttcacgc
    11221 cgccggttga gttggaaggc agggtgatgc cgcggcctac cgagaggcac tccaccagca
    11281 tccgccagcc ttgcccggcc attttcggcc cgccgatgat gtaatcgatc ggcacgaaga
    11341 catctttacc gcgcgtcggt ccgttctgga acggtacgtt cagcgggaag tggcgacgac
    11401 caatttccac gcccggcgtg gtggttggga tcagcgcaca ggtaatgcct aaatcttctg
    11461 caccgccgag taatttttcc gggtcggaga gtttaaacgc cagcccaagc acggtcgcaa
    11521 tcggtgccag cgtaatgtag cgtttgttcc aggtcagacg catccccagc acctgctggc
    11581 cctgccattc gcccatgcag acaatcccgg tgtccggaat cgcgcccgca tcggaacccg
    11641 cttccgggct ggtcagtgca aagcagggga tctcctgacc acgcgccaga cgcggcagat
    11701 agtgatcttt ctgctcgtca gtgccgtaat gttgcaacag ttcgcccggg cctaatgagt
    11761 ttggcacgcc gacggtaatc gccaggatcc cgctcacgcc agagagtttt tgcagcacac
    11821 gagactgggc ataagctgag aactccagtc cgccgtactc ttttttgata atcatcgcga
    11881 agaagcgatg ctctttaagg tacgcccaca actccggtgg cagatccgcc agctcatgag
    11941 taatctggaa atcattcgcc atccggcagg cttcttctac cgggccgtcg agaaacgctt
    12001 gctcttcagc ggtcaggcgc ggctgcggat agttatgcag ctttttccag tccggcttgc
    12061 cctggaacaa gtcgccctcc caccaggtgg tgcccgcatc aatcgcttct ttctcagtgc
    12121 gcgacatcgg cggcatcacc ttacggaaac cgcgaaatac cggcgcggaa atcatcgact
    12181 tacgcatagg cgcaaagtta aatggcacga ggataatggt cagaggcacc agtacccacg
    12241 ccgaccacag accagcaacg ccgagggcgg ctgtccaggc gagcaaaatc agactgctga
    12301 taaataagct cacgcggtga tagaacaacg cgccgagcag gacaaccgta gcgagaatac
    12361 tcaaaatcat cataacgaaa agccccttac ttgtaggagg tctgaccact tgtgatgata
    12421 tggttgtagt ggatgtaaaa acatttagca atgtgtttac aatataatta caacaaagct
    12481 cacattgttg ctgtttttat ccgcacttca ggtcaaaaag tcctggtcat agcacctgcc
    12541 cgtacttctc gcttttggcg gtatccggta cactgcattt tgtctattac atttatgctg
    12601 aaggatatcc tcatgtacca ggatcttatt cgtaacgaac tgaacgaagc ggcggaaacg
    12661 ctggctaact ttttaaaaga tgacgccaat attcacgcca ttcagcgcgc ggcggtcctg
    12721 ttagcagaca gctttaaagc cggtggcaaa gtgctttcct gcggcaacgg cggttcccat
    12781 tgcgacgcta tgcactttgc cgaagagttg accggtcgct accgtgaaaa ccgtccgggc
    12841 tacccggcga ttgctatttc tgacgttagt catatttcct gcgtcggtaa tgatttcggt
    12901 ttcaatgata ttttctcccg ctacgttgaa gcggtaggtc gcgaaggcga tgtactgctg
    12961 gggatctcca cctccggtaa ctctgcaaac gtgatcaaag cgatcgcagc ggcgcgtgag
    13021 aaggggatga aagtgatcac cctgaccggt aaagacggcg gtaaaatggc tggcacggcg
    13081 gatatcgaaa ttcgcgttcc gcactttggt tatgccgacc gcattcagga gattcacatt
    13141 aaagtgatcc atatcctgat ccagttaatt gaaaaagaga tggttaagta agtctggcgt
    13201 aggccggata aggcgtttac gccgcatccg gcacttgtgc gctgatgcct gatgcgatgc
    13261 tgacgcgtct tatcatgcct acaaatctgt acccgaaccg taggccgaat aattcgttta
    13321 cgccgcatcc gacctgaaaa ttcttaaatc aatcttcgcc ggggtccatg cgctcccgct
    13381 gttgtggagg ttacccatgt gcgaattgct cgggatgagc gccaacgtcc ctaccgatat
    13441 ctgctttagt ttcaccggac ttgtacagcg tggtggtgga accgggccac ataaagatgg
    13501 ctggggcatt accttttacg aaggtaaagg ctgtcgcaca tttaaagatc cacaacccag
    13561 ctttaattcc cccatcgcca aacttgtcca ggactacccg ataaaatcct gttcggtggt
    13621 ggctcatatt cgccaggcta atcggggcga ggtggcgctg gaaaatactc acccatttac
    13681 ccgcgagtta tgggggcgta actggactta tgcccataac ggacaactga cgggctacaa
    13741 atcactggaa accggcaact tccgcccggt cggtgaaacc gacagcgaaa aagccttttg
    13801 ctggctgctg cataaattaa cgcagcgtta cccgcgcacg ccgggcaaca tggcggcagt
    13861 gtttaaatat atcgcctcac tggcggatga actgcgggcg aagggcgttt tcaacatgct
    13921 gctttcggac gggcgctatg taatggcgta ttgctcgact aatttacact ggatcacccg
    13981 ccgcgcgccg tttggcgtgg caacgttgct ggatcaggat gtggaaatcg acttcagctc
    14041 gcagaccaca ccgaatgatg tggtcacggt gattgcgaca cagccgctga cgggcaatga
    14101 aacctggcaa aaaattatgc caggcgaatg gcgcttattt tgcctcgggg agcgtgtagt
    14161 ttgatgccag ttgtggctgc accacttcgt ggcttaacgg cttgctgacc acgtaacggc
    14221 cattggccac agaaacggtc ggtggcttac gggtttgctc aaagtagtcg tagcccggct
    14281 tcagctgctc ccagaagtcc ttaaagttgg aatatttatg gcgcttcata ttggcgtcgg
    14341 tcatgcggaa tgggtaaata ctcacctgca cgctcggctg accaaacacc agcgcaccag
    14401 taacgaactg gaagatctca tcaataccct gattggtcat tgcgtagcag ccgatggata
    14461 cacaatcgcc gtggatcatc aggtatttcc cttcataacc atgcgcacgg tcataagcat
    14521 tggggaatcc aatattaatc gctttgtagt aacggctgtc tggttttaac tgattacgct
    14581 ggacgctata aaacccttcc gggcttttga aatcgccctg acgctgtttt ggccctaagc
    14641 cgccggaata tttacagatt ttataactgt cgagcagttg atattgctcg cccattttga
    14701 cgtaaagatc gagcgtacgt tcttccttga agatctggat gtagacaggg gatcccatca
    14761 actgctgctt atactctttg ctcactggcg tggtcgagct actgctgccc agcaaaccgg
    14821 caaacgaaac gcacgggatc aacaacatcg caagaattaa tgcgatttta cgcatactgc
    14881 ttattccttg ataaaacggt tacacacgcc aggacggcaa aatggatccc aaatcggaat
    14941 agtctggatt tggaaggctc acattatcac caaaagagtt ttacgcaagc ctgtcggcgc
    15001 tgggtttaca aatttagcac aggggcagtt ttcaatagtc cgaaaagcgc atgtatcatt
    15061 tatttcctaa tattatgaaa tctcaatagt aatatgcaaa taagcaatta ataatcccgt
    15121 ttactcctcc ggtcttttaa aattctcacc ttcattcagc cggtttatgt ctttcatgtc
    15181 ttcgttcttg ctcggcagat ttttgcatcc aggcgtgttt tcgttatgcg ttttgctccc
    15241 tttactcgcc agcgccacca caaccgggag cagataaaac agcatccaga atggtttcct
    15301 gcgccgctca aggcgagtga cagacgctgg caggcgctgg cggaaaacaa ccacttttta
    15361 agcagcgatc acctgcataa catcaccgaa gtggcgattc accgcctgga gcagcagctt
    15421 ggcaagcctt acgtctgggg cggcacgcgg cccgatcaag gcttcgattg tagtgggtta
    15481 gttttttatg cctacaacaa gatccttgag gcgaaactcc cgcgcacggc caatgagatg
    15541 taccactacc gtcgggcaac gattgtggcg aataacgacc tgcgccgggg agatttgcta
    15601 tttttccata tccacagccg cgagattgcc gaccatatgg gggtgtatct gggcgacggt
    15661 cagtttattg agtcactgcg caccggggaa accattcggg taagccggtt agccgaacct
    15721 ttctggcagg accatttttt aggcgcgcgc aggattttga cggaagagat gattttgtag
    15781 gccggataag gcgtttacgc cgcatccggc ggttgtgcac ggatgcctga tgcgacgctg
    15841 acgcgtctta tcatgcctac aaatctgtgc gtttttgcga gccgctttcc cgatataaca
    15901 cagcgtcatt gtcggccttt ccttttcctc ttaccgttga gaaaaaggag tcgctatgtc
    15961 ggaatatcgt cgttattaca tcaagggggg aacatggttt ttcacggtaa atttacgcaa
    16021 tcgtcgaagc catcttctga ccacccagtt ccagacgctc cgtaacgcca ttattaacgt
    16081 taagcgagac agaccttttg aaattaacgc ctgggtcgtt ttgccggagc atatgcattg
    16141 catatggaca ttacccgaaa gcgatgatga tttttcctct cgctggcggg agattaaaaa
    16201 gcagttcacc catgcttgcg gattgaaaaa tatctggcaa ccgcgttttt gggagcatgc
    16261 cattcgcaat acaaaagatt atcgccatca tgttgattat atttatataa atccagagaa
    16321 acatggctgg gtaaagcagg tgagtgattg gccattctca acgttccatc gcgatgtcgc
    16381 gcgagggtta tatcccatcg attgggcggg agacataacg gatttaagtg ccggggagcg
    16441 tatcatttta taactgtgcg ctggtgccgg atgcgcttac gcttatccgg cctacaaatc
    16501 gtgctttaac ggtaggccgg ataaggcgtt cacgccgcat ccggcagtta tgcgcagatg
    16561 cctgatgcga cgctggcgcg tcttatcatg cctaaaaact tgtgcctgaa cggtaggccg
    16621 gataaggcgt ttacgccgca tccggcaatt gtgcaccgat gccggattct tacatcaacg
    16681 cccccataat cttcaactcc agctcatccg gcacttcgtt atacgacagc acatgcagcc
    16741 ccggcgcaaa caaccttgca taacgcggca gcaaagggcg tagctgcggc ggcaccagca
    16801 gcaccggatc tttccccgcc gctttcatct gctctttcac ctgtggcatc gtgctctgga
    16861 actggttgag catattcggg tcaaccggca cgctgtcgag catcactttg ccggcctgtt
    16921 gcgcctgatt cactacgttg gtcagcagat tttccagctc attattcagc gtatacaccg
    16981 tcagctcctg cttgcgaacg aacggatgag taatgctgcg ccgcagcgcc aggcgcacat
    17041 cggccgccag cagaatatga tctttagtca ccgtgctact ggcgaccagc acggtggcga
    17101 tggtgacgat atcgcgcagg gaaacgcctt cggtcagcag cgcccggtac actttcagca
    17161 attggctgta attgagcgcc gcgcttaaat cctccgccag gcgcggtgcc atcgacgaca
    17221 aacggttatg caactgcgtg atgtcatcat agttaaacaa atcaggaata tagctgcgca
    17281 caatcttgtt cacatgggtg gcaatcacac tggcgctgtc gatcacctga taccccatat
    17341 tcagagcctt cgctttctgc gcaggctgga tccaggtgac cggtatcccg tacgccgggt
    17401 cgttccccag cacgccgtca atctcgccgt aggtttcgct ggagggcagc gccatcagtt
    17461 tatccgccgg aatatccgct tcatcagcct caatgccgtt gatgaaaata gcgtactggc
    17521 tgggcttgag gcggaagttt tcccgaatgc ggatctccgg cagcagcacg ccgttaccgt
    17581 cggaaatcac ctgccgaacg ccgcgaatcc gctgggtgag cgggttgccc tgcgctttgt
    17641 ccaccagcgc caccagcttg tagccgaggc ttaagctgat gggctcgatc agcggaatag
    17701 tttcccaact gacctgttgt tcgctggttt cagtgatggt gcgggtcagc gtttcgaggc
    17761 ttttctcttc cgcctccgcc gcctgcgggc gtttgctcat ccgccagccg gtaaagccaa
    17821 gcagggcgct gaacagcaaa aacggcagat gcggcatccc cggcaccacc gccagcacga
    17881 acatgatccc ggtggcggtg tagagcaccg acgggcttgc cagcaactgg tggcgcacgt
    17941 catgggcgat atcgccgttg tcgctgacgc gggtgacgat aatcgccgcc gcggtggaga
    18001 gcagcaggga agggatctgc gccaccaggc cgtcgccgat ggtcatcagc acgtattgct
    18061 ggaaggcggc gtcggcgctc aggttgtatt tgaagatgcc gatacagacg ccgccgctca
    18121 ggttgatcgc cagaatcatc atcccggcga tggcgtctcc gcgtacaaac ttcgatgccc
    18181 cgtccatcgc gccgtagaaa tcggcctcgc tggcaacatc tttacgccgg gtttgcgcct
    18241 gcgcctggtt gatcaatccg gcgttaagat cggcgtcaat cgccatctgt ttgccgggca
    18301 tcgcgtccag ggtaaagcgg gcagaaacct cggaaatacg ctcggcctct ttggtgacga
    18361 cgataaagtt gattgtcaac agcgtcagta aatccgagcg tgaaagcatt atcgccgcgc
    18421 tgcacgggca gtcgattttt aacggtggtg ggctgtcgcc gttaaataaa atcagcccgt
    18481 ctcatccgcc aaaacctgcc accgtggcgg tgccggaaga gacggagaaa aaagcgcgtg
    18541 atgtgaatga gaaaacggcg ctgctgaaga agaaatccgc caccgagctt ggcgagttgg
    18601 caaccagtat caacaccatt gcccgtgatg cgcatatgga agcgaatctg gagatggaga
    18661 ttgttcccca gggattacgc gtgctgatta aagacgacca gaatcgcaat atgttcgaac
    18721 gcggcagcgc gcagattatg ccgttcttta aaactttgct ggtggagctc gcgccagtgt
    18781 tcgactcgct ctataacaaa attattatta ccgggcatac cgatgcgatg gcctacaaaa
    18841 acaatatcta caacaactgg aacctttcgg gcgatcgcgc gctttcagct cgtcgggtgc
    18901 tggaagaggc cggaatgccg gaagataaag tgatgcaggt aagcgcaatg gcggaccaga
    18961 tgctgctgga cgcgaagaat ccgcaaagcg cgggcaatcg gcgtattgag attatggtgc
    19021 tgaccaaaag tgcgtccgat acgctgtatc aatactttgg tcagcatggg gataaagtgg
    19081 tgcagccgct ggtgcaaaag ctggataagc agcaggtgct ttcgcagcga atgcgctaaa
    19141 tgctgaatct ttacgcattt ctcaaaacct gaaatcactg tatactttac cagtgttgag
    19201 aggtgagcaa tgcgtaaaat cattcatgtg gatatggact gctttttcgc agcggtggag
    19261 atgcgcgaca atcccgctct gcgcgatatc cctattgcta ttggcggcag ccgcgaacgt
    19321 cggggggtga tcagcaccgc caattatccc gcgcgtaaat ttggcgtacg tagcgctatg
    19381 cctactggga tggcgctcaa attatgccca catctcacct tgcttccggg gcgctttgac
    19441 gcctacaaag aagcctcaaa tcatatccgt gaaatcttct cgcgctacac ctcgcgcatt
    19501 gaaccgttgt cactggatga ggcttatctc gatgtcaccg atagcgtcca ttgccacggt
    19561 tctgcgaccc tcatcgccca ggaaatccgc cagacgatct tcaacgagct gcaactgacg
    19621 gcatctgcgg gcgtggcacc agtaaagttt ctcgccaaaa tcgcctccga catgaataaa
    19681 cccaacggtc agtttgtgat tacgccggca gaagttccgg catttttaca aaccttacca
    19741 ctggaaaaaa tccccggcgt cggcaaagtc tcggcggcaa aactggaagc gatggggctg
    19801 cggacctgcg gtgatgtaca aaagtgtgat ctggtgattc tgcttaaacg ctttggcaaa
    19861 tttggccgca ttttgtggga gcgtagtcag gggattgacg agcgcgacgt taacagcgaa
    19921 cggttacgaa aatccgtcgg cgtggaacgc acgatggcgg aagatattca tcactggtct
    19981 gaatgtgaag cgattatcga gcggctgtat ccggaacttg aacgccgtct ggcaaaggta
    20041 aaacctgatt tactgattgc tcgccagggg gtgaaattaa agttcgacga ttttcagcag
    20101 accacccagg agcacgtctg gccgcggctg aataaagctg acttaatcgc caccgcgcgt
    20161 aaaacctggg atgaacgccg cggcgggcgc ggtgtgcgtc tggtggggct gcatgtgacg
    20221 ttgcttgacc cgcaaatgga aagacaactg gtgctgggat tatgatgtat actattatgt
    20281 atattctggt gtgcattatt atgagggtat cactgtatgc atcgaattct cgctgaaaaa
    20341 tcggtcaata tcactgagtt acgtaaaaac ccagctaaat actttattga tcaaccagtt
    20401 gcggttcttt ctaataatcg ccccgcagga tatctcttaa gtgccagcgc attcgaagcg
    20461 ttaatggaca tgcttgctga acaagaggag aaaaagccca taaaggcgcg ctttcgtcca
    20521 agtgctgcaa gattagagga aattacacgc cgcgctaaac aatatcttaa tgatatgacg
    20581 gatgatgatt tcaatgactt taaggaataa ggatgcgggt attcaaaaca aaacttattc
    20641 gcctgcaact tacagcagag gaacttgatg cgttaacggc ggattttatt tcctataagc
    20701 gtgacggtgt tttgccagat atatttggtc gcgatgcact ctacgacgac tcgtttacct
    20761 ggccattaat caaatttgag cgagttgctc atattcatct ggcaaatgtg aataatccat
    20821 ttccgccaca gttgcgccaa ttcagcagaa cgaatgacga agcgcatttg gtatattgtc
    20881 agggtgcgtt tgatgagcaa gcatggttgc tcattgccat tctgaagcct gaacctcata
    20941 aactggctcg agataacaac caaatgcata aaattggaaa aatggcagaa gcgtttcgca
    21001 tgcgtttttg aatttatatt atgaataaca tacaaataag agattatcag cctggcgatt
    21061 ttcagcaact atgcgctatt ttcattagag cggttacgat gaccgtcagt cagcattatt
    21121 cactacaaca aattgccgcc tgggcgcaga ttgacgaatc tcgctggaag gagaaactcg
    21181 cgaaatcaca agtgcgggtt gcagttatta atgcaaaact ggtcggcttt attacctgcg
    21241 ttgaacacta tatcgatatg ttatttgttg accctgaata cacccgccgt ggggttgcca
    21301 gcgctttgtt aaaacctttt attaagtctg aatccgaact tacggtggac gcaagcataa
    21361 ccgcaaaacc cttttttgaa cgttatggtt ttcagacagt taagcagcag cgcgttgaat
    21421 gccggggagc gtggtttatt aatttttata tgcgttataa accgcaacat taaatccagc
    21481 ttgcaatgaa aataacgccc gcctagtatg tgcgcgtttt cttattccac aaactgcaag
    21541 gaggtaaatc atgacaaatc ctttatcaat gactctttgc agacctttcc aggattaatt
    21601 ctttttttct tgccctggat tcgtctgcca tttcctgatt ttttaattgt tactctcaat
    21661 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
    21721 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
    21781 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
    21841 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
    21901 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
    21961 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
    22021 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
    22081 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
    22141 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
    22201 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
    22261 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
    22321 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
    22381 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
    22441 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
    22501 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
    22561 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
    22621 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
    22681 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
    22741 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
    22801 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
    22861 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
    22921 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
    22981 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
    23041 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
    23101 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
    23161 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
    23221 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
    23281 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
    23341 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
    23401 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
    23461 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
    23521 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
    23581 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
    23641 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
    23701 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
    23761 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
    23821 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
    23881 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
    23941 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
    24001 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
    24061 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
    24121 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
    24181 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
    24241 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
    24301 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
    24361 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
    24421 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
    24481 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
    24541 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
    24601 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
    24661 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
    24721 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
    24781 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
    24841 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
    24901 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
    24961 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
    25021 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
    25081 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
    25141 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
    25201 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
    25261 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
    25321 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
    25381 taccatttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
    25441 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
    25501 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
    25561 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
    25621 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
    25681 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
    25741 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
    25801 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
    25861 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
    25921 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
    25981 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
    26041 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
    26101 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
    26161 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
    26221 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
    26281 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
    26341 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
    26401 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
    26461 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
    26521 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
    26581 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
    26641 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
    26701 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
    26761 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
    26821 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
    26881 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
    26941 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
    27001 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
    27061 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
    27121 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
    27181 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
    27241 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
    27301 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
    27361 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
    27421 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
    27481 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
    27541 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
    27601 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
    27661 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
    27721 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
    27781 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
    27841 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
    27901 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
    27961 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
    28021 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
    28081 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
    28141 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
    28201 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
    28261 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
    28321 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
    28381 aaccaaaacg gatataactc tggtcgccat ctttactgg
//
LOCUS       DAJTRH010000048        28404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTRH010000048 DAJTRH010000000
VERSION     DAJTRH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO339_002226"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO339_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5926840.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OO339_002227"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OO339_002227"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5926841.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OO339_002228"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OO339_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5926842.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OO339_002229"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OO339_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5926843.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OO339_002230"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OO339_002230"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5926844.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OO339_002231"
     CDS             6426..7055
                     /locus_tag="OO339_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5926845.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO339_002232"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO339_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5926846.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO339_002233"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO339_002233"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5926847.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OO339_002234"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OO339_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5926848.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="OO339_002235"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="OO339_002235"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5926849.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="OO339_002236"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="OO339_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5926850.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="OO339_002237"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="OO339_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5926851.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="OO339_002238"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="OO339_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5926852.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="OO339_002239"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="OO339_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5926853.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="OO339_002240"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="OO339_002240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5926854.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO339_002241"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO339_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5926855.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO339_002242"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO339_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5926856.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="OO339_002243"
     CDS             complement(21746..22624)
                     /locus_tag="OO339_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5926857.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO339_002244"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO339_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5926858.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25252..25587
                     /locus_tag="OO339_002245"
     CDS             25252..25587
                     /locus_tag="OO339_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5926859.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25745..26188
                     /gene="yjdN"
                     /locus_tag="OO339_002246"
     CDS             25745..26188
                     /gene="yjdN"
                     /locus_tag="OO339_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5926860.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26321..27109
                     /gene="phnC"
                     /locus_tag="OO339_002247"
     CDS             26321..27109
                     /gene="phnC"
                     /locus_tag="OO339_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5926861.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27134..28150
                     /gene="phnD"
                     /locus_tag="OO339_002248"
     CDS             27134..28150
                     /gene="phnD"
                     /locus_tag="OO339_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5926862.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28256..>28404
                     /locus_tag="OO339_002249"
     CDS             28256..>28404
                     /locus_tag="OO339_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5926863.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7296 a         7418 c         6935 g         6755 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgtaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctag atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccacgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgc atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 tgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctga
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgaa actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac ctatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgttttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca gaaagataaa
    25021 aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat
    25081 agaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta
    25141 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
    25201 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
    25261 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
    25321 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
    25381 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
    25441 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
    25501 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
    25561 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
    25621 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc
    25681 agcctctcat ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac
    25741 catcatgccg ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta
    25801 ttatcaacgt acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa
    25861 atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat
    25921 cgctcatgcc aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc
    25981 aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg
    26041 aaaacgctgg tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac
    26101 tttctgggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa
    26161 tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca
    26221 tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata
    26281 atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt
    26341 cgagaagctc gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat
    26401 tcatcacagt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt
    26461 acgtcattta agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact
    26521 gggccgcact gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa
    26581 caccggctac atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt
    26641 gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg
    26701 cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca
    26761 tcagcgcgtt tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct
    26821 gatgcagcag gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc
    26881 agcgcgcatc gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt
    26941 cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg
    27001 tcaggggcac gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca
    27061 tctctaccgc agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca
    27121 ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag
    27181 cctcagcacc ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg
    27241 cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga
    27301 tatggagaag aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat
    27361 tatccagggg atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat
    27421 ggaagcggtg gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc
    27481 gccgggttac tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga
    27541 tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg
    27601 cttcctcgtc cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa
    27661 gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca agcaagtgga
    27721 tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct
    27781 taaagagctg aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg
    27841 caagaatctg tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa
    27901 aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag
    27961 cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa
    28021 aggcaataaa gggctgaatg agcaggacaa gctggcgaaa accaccgaga ttcaggcgca
    28081 gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg atgagttcgg tgagtaaagc
    28141 ggtgcagtaa atcgtaggtc ggataagacg ccccggcgtc gcatccgaca atgtgtaggc
    28201 gttgatgccg gatgcggtgc aagcacctta tccggcctac agaccggagc caaacatgca
    28261 aaccatcacc atcgccccac ccaagcgtag ctggttctcg cttctgagct gggccgttgt
    28321 tctcgacgtg ctggtcgtct cgtggcaggg cgcggaaatg gccccgctta cgctgattaa
    28381 agacggcggc aacatggcaa cctt
//
LOCUS       DAJTRH010000049        28193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTRH010000049 DAJTRH010000000
VERSION     DAJTRH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(55..1347)
                     /gene="spaL"
                     /locus_tag="OO339_002250"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             complement(55..1347)
                     /gene="spaL"
                     /locus_tag="OO339_002250"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5926864.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            complement(1351..1752)
                     /gene="spa15"
                     /locus_tag="OO339_002251"
                     /gene_synonym="spaK"
     CDS             complement(1351..1752)
                     /gene="spa15"
                     /locus_tag="OO339_002251"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5926865.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            complement(1765..3825)
                     /gene="mxiA"
                     /locus_tag="OO339_002252"
                     /gene_synonym="virH"
     CDS             complement(1765..3825)
                     /gene="mxiA"
                     /locus_tag="OO339_002252"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5926866.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            complement(3838..4905)
                     /gene="mxiC"
                     /locus_tag="OO339_002253"
     CDS             complement(3838..4905)
                     /gene="mxiC"
                     /locus_tag="OO339_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5926867.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            complement(4924..6624)
                     /gene="mxiD"
                     /locus_tag="OO339_002254"
     CDS             complement(4924..6624)
                     /gene="mxiD"
                     /locus_tag="OO339_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5926868.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            complement(6611..7243)
                     /gene="mxiE"
                     /locus_tag="OO339_002255"
     CDS             complement(6611..7243)
                     /gene="mxiE"
                     /locus_tag="OO339_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5926869.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(7373..7801)
                     /gene="mxiM"
                     /locus_tag="OO339_002256"
     CDS             complement(7373..7801)
                     /gene="mxiM"
                     /locus_tag="OO339_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5926870.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(7785..8192)
                     /gene="mxiM"
                     /locus_tag="OO339_002257"
     CDS             complement(7785..8192)
                     /gene="mxiM"
                     /locus_tag="OO339_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5926871.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(8185..8880)
                     /gene="mxiN"
                     /locus_tag="OO339_002258"
     CDS             complement(8185..8880)
                     /gene="mxiN"
                     /locus_tag="OO339_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5926872.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(8822..9349)
                     /locus_tag="OO339_002259"
     CDS             complement(8822..9349)
                     /locus_tag="OO339_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5926873.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(9346..10071)
                     /gene="mxiJ"
                     /locus_tag="OO339_002260"
     CDS             complement(9346..10071)
                     /gene="mxiJ"
                     /locus_tag="OO339_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5926874.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(10077..10370)
                     /gene="mxiI"
                     /locus_tag="OO339_002261"
     CDS             complement(10077..10370)
                     /gene="mxiI"
                     /locus_tag="OO339_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5926875.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(10383..10634)
                     /gene="mxiH"
                     /locus_tag="OO339_002262"
     CDS             complement(10383..10634)
                     /gene="mxiH"
                     /locus_tag="OO339_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5926876.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(10642..11757)
                     /gene="mxiG"
                     /locus_tag="OO339_002263"
     CDS             complement(10642..11757)
                     /gene="mxiG"
                     /locus_tag="OO339_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5926877.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(11766..12224)
                     /gene="ipgF"
                     /locus_tag="OO339_002264"
     CDS             complement(11766..12224)
                     /gene="ipgF"
                     /locus_tag="OO339_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5926878.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(12224..12586)
                     /gene="ipgE"
                     /locus_tag="OO339_002265"
     CDS             complement(12224..12586)
                     /gene="ipgE"
                     /locus_tag="OO339_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5926879.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(12596..14212)
                     /locus_tag="OO339_002266"
     CDS             complement(12596..14212)
                     /locus_tag="OO339_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5926880.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            14526..16010
                     /gene="icsB"
                     /locus_tag="OO339_002267"
     CDS             14526..16010
                     /gene="icsB"
                     /locus_tag="OO339_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5926881.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            16023..16412
                     /gene="ipgA"
                     /locus_tag="OO339_002268"
     CDS             16023..16412
                     /gene="ipgA"
                     /locus_tag="OO339_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5926882.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            16428..17054
                     /gene="ipgB1"
                     /locus_tag="OO339_002269"
     CDS             16428..17054
                     /gene="ipgB1"
                     /locus_tag="OO339_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5926883.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            17111..17578
                     /gene="ipgC"
                     /locus_tag="OO339_002270"
     CDS             17111..17578
                     /gene="ipgC"
                     /locus_tag="OO339_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5926884.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            17584..19326
                     /gene="ipaB"
                     /locus_tag="OO339_002271"
     CDS             17584..19326
                     /gene="ipaB"
                     /locus_tag="OO339_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5926885.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            19346..20437
                     /gene="ipaC"
                     /locus_tag="OO339_002272"
     CDS             19346..20437
                     /gene="ipaC"
                     /locus_tag="OO339_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5926886.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            20488..21486
                     /gene="ipaD"
                     /locus_tag="OO339_002273"
     CDS             20488..21486
                     /gene="ipaD"
                     /locus_tag="OO339_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5926887.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            21495..23396
                     /gene="ipaA"
                     /locus_tag="OO339_002274"
     CDS             21495..23396
                     /gene="ipaA"
                     /locus_tag="OO339_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5926888.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            23400..23636
                     /locus_tag="OO339_002275"
     CDS             23400..23636
                     /locus_tag="OO339_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5926889.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            23853..24782
                     /gene="virB"
                     /locus_tag="OO339_002276"
     CDS             23853..24782
                     /gene="virB"
                     /locus_tag="OO339_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5926890.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(25729..26508)
                     /gene="ipaJ"
                     /locus_tag="OO339_002277"
     CDS             complement(25729..26508)
                     /gene="ipaJ"
                     /locus_tag="OO339_002277"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5926891.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(27108..27245)
                     /locus_tag="OO339_002278"
     CDS             complement(27108..27245)
                     /locus_tag="OO339_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926892.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            27861..>28193
                     /locus_tag="OO339_002279"
     CDS             27861..>28193
                     /locus_tag="OO339_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5926893.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQE"
BASE COUNT         9557 a         5764 c         4146 g         8726 t
ORIGIN      
        1 tgtgcttcaa ttaatttaac agaccgttga tatttatctt tgatttttaa aaccttatct
       61 aattgtttca ccaataagct ccattgtctg ctcataagta aaaccgagtc gataatcttg
      121 cttcagaaaa ctctcaacaa cagatatttt gttgtagatt ttatcctgag aggcattctc
      181 ccccggtttg tattcaccaa agtcaataat tgtccttagc tcttctattt cagaaagtag
      241 ctcccgaaat gcagcggcca taatacgatg tttttcatca acaacctgtg taaataccct
      301 gcttatactt tttaaggaat caattgcagg aaattgtcct ttttgggcta gatttctgct
      361 caaatatata tgtccatcta aaatggatct tacctcttca gctaatggat cagcaaaatc
      421 atcatcctcc aaaagaacag tgtaaaatgc agtaatagag ccacctgcct ttaactttcc
      481 tggcctttca agaagtctgg gtaagctatc aaaaaccgaa accggatagc ctcttctggc
      541 aggtgattct ccagcggcta aggccacatc acgtaatgct ctggcatacc ttgttaatga
      601 atcaataaaa agcgctactt tatgtccttc agtcctaaaa aattcggcta tagcagtggc
      661 tatatatgca gcattacaac gatcaaccga agagtaatcc gaagttgcat atactaaaac
      721 acacctgctt tttttctcag agtttttcaa ataatcaacc gtttcagtaa cctctcgacc
      781 tcgctcacca attaacccaa taacatatat atcagcacca ctatgttcaa tgagcatatt
      841 catgagaaaa gttttgccac aaccagctga cgcaaaaatc cccattcgct gtccttcacc
      901 acacgtgagt aaagaatcaa taaccttaat acctgttaaa aaaggcttct caattgcagc
      961 cctttcacta tatagcggag gagcattatc tacaggtcga taaagaattt cactgttatc
     1021 tgtaacagca aacttatcag taacctcccc taaaggattg actactgctc ccaataatcc
     1081 acggccgact tgcgtatgaa gaaactgggc agtgggctta atcaacgttt gccgtgaaag
     1141 tccacgagaa tttccaatca agcttaatat tgttttttca tcatgaaatc ctacaacctg
     1201 agctcttgca acaatttcat tactttcaat tccagcctga atgttacaaa tctcaccaat
     1261 cgaaacatcg ctaagacttg tttccaagat tggccccgag attctattag gaaaagataa
     1321 ttgagtgagc aattttgtat agctcattga ttataagacc ccatttaaga tttccatcct
     1381 ctgataaaac tcatgcagaa tctctgcaaa aacaatccca tcgtgaacgt agtcatcttt
     1441 aataacaact cttaactgta agtactcatc agaacgatga agctctacca gctcattaat
     1501 gctataacta aagttcatta acattaaatt taatatgtta taagcagatg actgaagttt
     1561 tacatcactt ggtgcatcaa aattagccca taacataacc tgctcattga caagagctat
     1621 attaatagca ggcatactat ccaaagaaat ggtaatcgct gaatggctgt ctaaatctgt
     1681 aataattgat ggaggacaac caatcgtgaa aagactatct ctaactaatt gaactaaatt
     1741 aatgttactc atatttaaac ctcactaaat agtctttaat acattaatgg tatatgcttc
     1801 atcaatctca gcatacgata taacgagtat actcttaaat ctgttatcta taagtctttt
     1861 aacaaaccta cgtatatcta ctgataccaa aagaacgaaa tttttttttg catttctcaa
     1921 ttctctcaaa gcatgtgcta aagtttccat tacctcatcc gaaacctcta tatccatatt
     1981 caagaaagag ccaccagagg tttgccttat cccttttctt attgcatcct caatatatcc
     2041 ggaaagcatc acaactttaa tctcaccaga aacagctatt ttactacaaa tatacctaga
     2101 aagtgatgca cggacatgtt caactaatgt tatgacatct ttttctctgg gagcccaaag
     2161 cgccaaagac tccataataa gttttaaatt gcgaacagaa atattttctc caagcaatct
     2221 ttgtaatacc tcagaaattc tctgtatagt cacatgtcgg aagacttcct ttaatagatc
     2281 aggataccga ttttcaaact gatctaacat attttttgtt tcttgtatac caaaaatctc
     2341 atttatgttg tttaataaag cttgtgacaa ctggtgataa aattcatctt gagcactttt
     2401 aattttagca tcaatattag ttagattttc tgtatatgaa acgtctaccc aagaaataac
     2461 acgttcatta tatgaagttg aaacaacagg aatacctaga gcatctatat ctccattttc
     2521 atctgtaata cacactttgt caaaatatat attaaaacta tcggcacgca cctcatttat
     2581 taacaaaaca atatcatcga ccttaagctc attgcttgtt ctatataaaa tagttggaag
     2641 cctgacacca taatcaataa agaactgact tcttatcctt tcaataagac cttccatatc
     2701 atttgcattt atcttatttt cggcaaataa taaaattaaa ggaacggttt ctgaactaat
     2761 agcatccaga ttttctatca ttgccaaaga tgagtcatgg ctattatcaa tatcaaatgt
     2821 accagtatag ccactagaat cagactcaga cagacttttt tctttttcta cgaccttttt
     2881 ataatagaat aaagccgtca aagtaactgc tatcaggaaa aaaacaaaaa acggaaaacc
     2941 tggcaacatc cctatcgcca aggcaagagc tgatgtaaca atgagaacaa aaggattccc
     3001 aaatatctgg gacattatgt tacgtcccat attatcgcta tcaccattaa ctcttgtaac
     3061 gataaatcct gcacttatag aaattaacag agcaggaatc tgagagacca atccgtcacc
     3121 aatagttagt atggtataag tagataatgc accagatagg gacattccat gttgactcat
     3181 accaacagaa atacctccta ttaaattgac aaaaataata atgatgccag caattgcatc
     3241 acctttaatg aacttcattg ctccatcaaa tgaaccatac aattgactct cacgttccag
     3301 aatactgcgt ctttctttag ctcctgcggc atcaataatt ccagccttta aatccgcatc
     3361 aatgctcatt tgtttccccg gcataccgtc aagagaaaag cgagcagcta cctccgcaac
     3421 cctttcagat ccttttgtta taacaatgaa ctgcacaaca gttactattg aaaaaataac
     3481 aaaacctaca gccagactat caccaatgac aaactggcca aatgtagtaa taattttacc
     3541 cgcatccgca tcaactaaaa ttagtcgact agtacttatt gataaagcga gacgaaataa
     3601 tgtcgttatc aacaaaaccg aagggaatgt agaaaaactc aatattcttt caatataaaa
     3661 tgatcccata aaaacgagaa tagcaagtac aatattaagc cctattaaaa aatcaacaag
     3721 gtatgtaggt aatggtatga tcaacatagc aatgatcata accataagca ctaatataat
     3781 taattcaggc ttagtactta cttgcttaag aaaagactgg atcactttta tctcctgtta
     3841 tctagaaagc tctttcttgt atgcacttgt gacaagctct cgtagaatat tgatcgcaat
     3901 ttctctttca ctttcacttt taaatatatc atgaggactt tcattaacag aagatagata
     3961 tgtttgatac actacagaat cctcatgact ggctcgtgat acttctataa cagaagtaag
     4021 aatttgctca ctttcacttg gatattgaaa aatatataat aaagttagca atagctgctc
     4081 tgcacttaaa ctgccatctt ttagcaagct tgaggattcc agtttcttca taaaaattac
     4141 gtctgaagtt ctaatcatct taattgcaat taatttagat aacacaaaac caaactcata
     4201 agcattacag ctcggtttct cagaatccat gtctacaatc agtgactgct ctacaaaatt
     4261 cacaattgta tgtctgtgat taaaaccaaa attaccaagc cattctatat actgatctgt
     4321 tgttgatatg ttccccatga taaaaccacg ataacatgca cgcaaaagct gtggttcaag
     4381 tttcattttg ttgccaaata atttagcctg tatagctgaa tttattccag cattaacact
     4441 tttttcacta cctgatatta tctcattaat aattttagtt aataattcga cttgttcagc
     4501 agtaagatct ttttcattca aaagttccct taatgctaag acttgatcac ttggatcttt
     4561 aaaatatctc tttagtctgt ctataaaatc ccgatctaga ggaaggttat cttttaacgt
     4621 tctctttaaa tcaaaaatct tgtgattaat ttcatcttct tccccatcta aaatacgttc
     4681 ctgactatca ctatttgttc ctttcagttt ctcaaggtct cttctgttta taaatgacga
     4741 aagagcggaa gccatctcat cagatgagtc aatatacttg ctattagcca agccagaatc
     4801 aagctctgca tcagcagtct catctccatc atctgaattt gtcattgcat tcaacttatc
     4861 aatgaatgca gagctaaaaa ctcctgtatt tttaacatca agcatacatc acctacttta
     4921 tagttagtaa tttaagtatg aaaccaatga tttttcgtct tccagcaaag tagtttcaga
     4981 tgggattatt tgtgtagtct tttgtatttc tctttcagat atgaggcttt tatattctgc
     5041 agtattataa taacttgact cctttatctc acggggttga atcagaaaaa cccttacaat
     5101 attactgata ttacttgttt tatacttgaa cacattaccg attacaggaa tagaggagag
     5161 aaaaggaata gatattattt cgttagagtt cgtttcatgc gtataaccac ctatcaacaa
     5221 acttttgcct tgtggaactc tcgctattgt actgattttg gtccttccaa cttctggaag
     5281 gacagatgta ttttcattat tataattgta atttgactga ctatttcctg ttccatcctc
     5341 tatcgttaaa gacatctcaa tttgtccccg agatgaaaag cgaggtataa catttatcaa
     5401 tgttccatac gtaacatgct ccaacgaaga attgcgctca ccaactaatg acacataaaa
     5461 agtcctgtta ttatcaaata tagccggaat attctcttgt gtcaaaatca ccggccttga
     5521 aactacattt gctttctttt tctgatttaa tgccataact gaagcaataa acttattgcc
     5581 gtccaaagtg cttatcgatg ctgatgagct catattaaat gacgcaccaa acgaatcacc
     5641 aaatgaagcc gttccttgcc aattaacacc aaggttattt aattcagatt tatcaatatc
     5701 gattatccat aatgataatt ctatatgtcg tttggctata tctaactgag tgataatatc
     5761 gcgaataatc tgtatttgct gatcattacc tttgactaat atagaattgg tttctggata
     5821 tgcaataagt gatacatcct ctaatataga actattggta actgaactaa atgaaaagtc
     5881 attactatcc tcagatactt tctgtgtaat attaaagggt ggcatcggat cattttgggc
     5941 ttgtctgtta ctcagagcct taccattatt taataaacgt tctacaacag tagccacccc
     6001 tgggatgaca atatcctccc ctctcatatt ataagttctg tcactaacaa atgtattttt
     6061 taattttata accccaaaat taactttatc tgtcccaata gaagaaacct gcttatcaag
     6121 caaagttgct gtattagcta ccagttcaac caatgccgga ggaccagata tatagaatgt
     6181 tttatcacta atgtttcctc ttataggata tctgtgatca taaaggttag catccttcag
     6241 atactgaata agataattaa gtgatatatt ctccagcaaa ataactttgc ttattaattc
     6301 tccagagtca tatatgtata atgcatttcc atctttatac catatgagtc ccacaagtaa
     6361 tgtcagtttt tcaagcattt cttctggatt agataaatca aactccccag atattctctt
     6421 ttttgcagct tgtttgctaa caacaatagg ataatttaat aacgcagaaa aacgttcaaa
     6481 aaaagatcct accgtatcac tttgagcaac atatttcgct atgtcatttt gttctaataa
     6541 atgagagtca atattattag cattaactat caatggtaac aatacaatca agagagtcaa
     6601 agatttaata ttaaattttt tcatttattt ttttcactaa aaaagtaata ttcgataatt
     6661 ctcttgcaga aaagcctagt cgtgttttta tctcatttga aaaatgggat gttgatgcat
     6721 acccattatt catggcagct gaagtgattg tttgattgtg caaaaataca tctaataaac
     6781 catttactaa ccgccaagtg ttcagttgtt ctttgacttt tgcccctaaa gcttttctac
     6841 ataaagatct aaaataagcc tccgacacac cataatgctc tgtcagactc tttattctta
     6901 cctccttttc tttctcaata gttctcagaa gataaagaac caaatgatat gactcagtca
     6961 ttcgtatcat agccagtgca ttacttacat tctggtcaga tgaaaagatg aatcttaaca
     7021 ttaacttatc aagcatcgat tcggtattac aactatcccg aagatcattc tcatgataga
     7081 aatgaataca tttttcagca tggctatgct ctactagagc accactgaaa tagtcccaaa
     7141 aagcaagaaa ttttgcacaa ctattaatgt taaagcgata atgatagttc acattatcac
     7201 tgaattttat attttgattt cggacaaaaa aaaacccttc catgctggag ccagctcaat
     7261 tatgttacgt tctttatctt ttacaagctc aacgttaact ggttcatgtc cactagagta
     7321 aatgtaaaac atattacttg tatttagacc tttatattta ctcacaatac aattatatcc
     7381 ctcgaattaa ttttaattta acttccccat ccttctctat aaggatggta tcatttatct
     7441 tattaccttt taaaacaacc ccgtctttca gcatagaaaa tagtttatct atagctatct
     7501 tttgtgcatc catgaagcac ccatctactt ccttaacatt aaagttgccc atgaatttac
     7561 ctgcattaaa actataaacc gccttaccac taatacattt agagtaaaca tttatacttt
     7621 tattggttgt attaaacgac cataataaat catttggaat agaaaaataa tccttactta
     7681 caggaacaat atgccattct ttttcagaat tagatgatga ctttaaagca cacccactca
     7741 gtgttaatag caatatactt aaaataaata ttttcaactt attactacca tgtcgaatca
     7801 tctgcctctc tccctgcttc aaagacacta attaatgaat cgaaggttgt agtcttaata
     7861 tagaaatcaa tattgttata tatggacaat gatgcataaa atccctcatg caaatcgaca
     7921 tattgatttg catctatgag tttattaaga agtgaacttt tttgtgcagc tgtaagataa
     7981 ggaaaacgtg aattgaataa ttgttcaatc ttattgtgca gttcatcatt atctatggaa
     8041 tttaattcac tctgtgagaa atattgcatt atttcataga ttatatcctc atcaaatgat
     8101 tgcgaagatg ataatctctc atttaaatta acaacttctt cactatttaa tctttgctgc
     8161 agagcattgc ttgcatttat ttgattaatc attaacagga ttctctttta acaagtcttc
     8221 tgcaatttgt ctcagtttct tatgtgctaa ctctttgaaa tattttttat cattttttgt
     8281 taatttttcc gcaagttcta ttttaacacc tgatattaca tcctgaggac taaactccac
     8341 tacttgatta ccactacaga atatataatt attcccttca gaatatttta aaataatctt
     8401 gacatctgag cgatatttat ggagatcaag ttctaacttt ttcctcagtg ctaaattaca
     8461 cttgggcaaa accactttta attctgttac tgtattacgc aacgaactaa gccactgctc
     8521 caataaagtg ctaatgatcc tctcatcatg taaattttct gaaagcaaac tagttatata
     8581 gtcctcaata tttcttctat tttcattttg ctttaacttc cattcacaaa tatagtcaat
     8641 aatatgctca aaagcagtct gtataccaat ttgataacca tcacatacag catctatacg
     8701 cagtgattca gcttttttcg tagcctcctt tataacacaa attgctttct tcttactctc
     8761 agtgtctcta tcttttatgg ttttacataa ttccttttca attctcttta taacaacacc
     8821 atcataggca tgatgtctgg ataccggtaa agtgcctttt tgcatattgc ataccttcat
     8881 ttaatcggga caaatttctt tctacaggcg tataatttcc ctccaaagca attggattaa
     8941 ataataagtt tattctttca tatataccaa cagatatatt ttccttccac gcaataagat
     9001 gccaaaaacc acttcgaata gcggcctccc cttgcttact gattttgccc ccataataac
     9061 taataaccat ctcactatga ttaattaacc gagactcttg actaccgata aaataagcaa
     9121 cagctggaag taaattccag ttggcaacaa acatagcggt taccccgctt aaaggttcaa
     9181 tatcgcaatt tagatcatat ttattaatta tgagattatt aatctcactt ctgattacgc
     9241 cattttctaa cagttcagaa ggcaaattca accgatttct atttatatag aacgctggat
     9301 caaaaattat tgaaagatat tttttataaa ttccatccat tcttatcata ttttgtttct
     9361 cttgaaccac cctgttttga atgcccatac caaaagaatg acaactagta cagccatccc
     9421 gagaaataaa tatattactt catttgttaa aaattccgat ttaacttcct taacaggttg
     9481 tacatttgta taaacatatt cttctttcgg agttaatatg acagatatat tttcatactt
     9541 aacatcagaa aaggtgtttt tcaaaaatcg cttaatatta ctaactaata gttcagactc
     9601 tttcggtgag tcatatatag cgattactga tatatgcatc ggttttgaag atatattttt
     9661 ttcttcaaga tcatagctaa catgtatttt tgccgaaata acaccaccaa tagaaactaa
     9721 agactgttcc agccgttgct caatagcact atataaacgg gccttttcag ctcttggaga
     9781 agacactaat gaatctgtag gaaacatttg tgagatatca actctctccg gatttggcaa
     9841 atcgtacatg cgcatcaaat caactgccga tgcaaatgtc cccttttcga cttgtaccga
     9901 gatcccctgt ttacctccat caacttttct agcagtaata ttatggcgtt ctagcacaga
     9961 tattatttca tttgcctgtc tttgagataa attagaaatt aactcttcac gttgctcaca
    10021 tccaatcaac atcagcaaca agaataaaat aaaaccttta tacctaatca tataaattaa
    10081 gactttaata aagtttcaac agccgaaact gtttttcttg ctaacgtgcc agctaatgat
    10141 actcctattg aataatttga gagcgtcgtc tgtaacttgg ccaaagattc tgggtttaat
    10201 gattctggat tgcttaccat ctccattatt tgtgatactt gaatatctgt atccatctta
    10261 atatcactat atttagccga cacgacgtct tccagacttg atatctcttg agattgaaaa
    10321 tcactggctt tgataatatc aacctgattg actggataaa tgtaattcat gaatgctccc
    10381 tattatctga agttttgaat aattgcagca tcaacatcct taatcacttt cactgtattg
    10441 gattgcgcgt tcctatataa tgtatattca gataatttac tttggtattc agccagcaac
    10501 tgtggattcg aaggattttt agctaattta tctagtgcca atgttagttc accttgtaat
    10561 gtttgagttc catcatcaaa agtttcagat aatgaactca atgtccaatc atcattcggt
    10621 actgtaacac tcattttatc ctcacttatt tttatctaaa aaaaaccagt gtttatcatt
    10681 caacattaca taactgtctt tgctgttaag atatgattta cctttaaaat catcatctat
    10741 caaaagtaca gaaaactcaa tatactgatc tccatatata tttttatgtt cagaaatcag
    10801 tctattaatg agctcaagct tttcatctgt cagttcttct ctgacagaat atgtaacttt
    10861 attattttca caaatctctt tatactggac atttaccttt gtgaaaatac ctttagcatc
    10921 cccccttgca ttatgatcgc taagatagtt gaatttaata tttttgatat acggaaattc
    10981 aacaatcaga gcatttttca atttatcaag ttcggtatct tttgaactat ttctttcttt
    11041 acttaaaaac accagtaatt ccgctttatc actcactaaa ttcaacacat acaaattgat
    11101 aaatgggaat cgttgattga tatattgttg aattctattt atttctttgt ttgacaccag
    11161 aatatatctg catttggtaa atccggtttt atttaaggct acagacgccc ataccgcatc
    11221 attttgtgtt tcggcaagaa tataattgca ctgtccagtg ttgactattc catatctctt
    11281 atcatctata atttcagcaa tttcttttac ttcatctttc tttaaagaga gggaaaaaat
    11341 tataattaac acaacaataa cagcaaagaa aaaaaacacc gagtggttct tatacattcc
    11401 gtttagtata tggcctctcg atttatcctc cctcatattt ttaaggtgaa aagaaatccc
    11461 gtcatattca aatgaattaa attgtgaatt gatatcaatt tgctcacctt tttcatcact
    11521 aaattgacag atattatctt tgcttatggt caaataaatt ataccatccg atttactatc
    11581 tgttacagga ataatatttt ctgggaaatt gtcgtttcca aactcaagtg tttctatcgt
    11641 ccgtccgaga acaattaagt tattcccata atataacgga aactcatccc cgacaccgtt
    11701 tgtgagtttc actaataacc tgaatggtgc aagatttgag ttctttgcct cagacataaa
    11761 tcctcttata tccttcgatt attctgcttg ctctctgctg ctatccgcaa atagcgtcta
    11821 tagatatcct ctgcgtactt taatctttct tttttctttt tcggagacgt tcctgcatta
    11881 taggctccaa cagcctccca ccctcttcca tacatcatca taaactcatt taataacttt
    11941 gctgcataat gtacagaaag acatggatga ctaattagca tttcctcaga atatcccatt
    12001 tctctaagtc ttttagaatg aaagtcattt atttgcatta taccataatc tttacttcca
    12061 ttgttattaa cattgactgc agatttatta aatccggatt ctttttccgc aatcgctttt
    12121 aacaaacttg atggaatatt gtacctttca ccagccttat cccaacaatc agctctccct
    12181 aaatatggga taaaacacag caaaataaat acgaaacggg acattaatac cccttcattc
    12241 ttcgcgcaaa ttcatccttc agccaacgca cacgcgaaat ataatattct gtattaacat
    12301 atacatcctc gaattcattt atcccggtaa gatctaatcg cgcaattaga tttccgcctt
    12361 catcatcagt agctaagcat atcttttctg agtaatttag gctcaacgca tatataagat
    12421 catttatatt ttcaggcaat gcacaaaaag ggcataataa atttattgaa tctccttctt
    12481 tttcaatata tattagcaca tcatcatcaa gcatgattgc ttgatcatcg atatcaatta
    12541 aagggatacc caaggctcgg caaataacat ctgctaaatc ttccatatat tcctcttata
    12601 caaatgacga ataccctttc accatattcc atatttttgg gtcccctatt ctttcagaat
    12661 aagatagctc taacgaagat agcggcaatt ttttcatgac tttatttccg ggcacaccag
    12721 tattcatctc ttggatttcc atattaccac tattcattag aatagtagaa aataatcttt
    12781 tctcttctga ggataattta ctatttaatt gagaaaactg acctgtttca tgttttctta
    12841 ttatttctct tttaatttca gcatcctgca tacctgttct gtccttccca ctcttacaat
    12901 tccagcaagg tacagcacct atagtataag ccaatagagt cattctttgt gacaatttat
    12961 acggctctcc attatcattt ttttgtaact ttttggttac aatctctttt atctggttag
    13021 ccagatatat cacatcattc ttacatggtg gatttttttc tattgcttct gcagcccaac
    13081 ctccaatcac accatttttg aggaaattat cccccaacaa agaacatatt gattcatcat
    13141 taagcttgtc aacattcctc cagcctaagc ccatttttag cgctaattca tttacaccaa
    13201 agttaaatgt caccactttt agattaacgc ttacttcttt cagaagacca tcactattcc
    13261 gaattaataa tttcgttggc ttcccccttt tagagtttaa cccttttaat gcgttcacct
    13321 gatcttttaa catactctcc tctcccccgg ttaaactggt cggcgtcaga agagaagtcg
    13381 aaacaatctt taaatctact gttttaccag acaaagcctg tgataataac tctggtctgc
    13441 tatataatgc tgcgcttact aactcctcag ctttattacg agcagcaaca gctctttcag
    13501 atgagttttt tttcaatcca taggctgata taactccatg tctgataccc gaaaaaattt
    13561 ccttcccctc atcatccacc acttttgaaa gccacatatt cgcgatatga tcactctctc
    13621 tcgtagaagc acaacaaatt ccttttccat tatactcttt gacaaatata tttttattcc
    13681 ctatcttcat atgagaggca ggagttaatt gaaaaccata atttttccca tgatgactaa
    13741 tattcttgtc gaccggtccc caattcttat tatttaaata atcagtaaat atttgtttta
    13801 ttttcgtttg caattccttt ttatttttaa tgcctagctg tgctcttatc tcttcactta
    13861 ttactttatt aagctcatta atatgtcttt tattaacttc accaagttta taggtatcta
    13921 atcgttccat catggcttta atttttgacg tatcaccacc tgccaacttt attgcctcgc
    13981 aaaaaacttc agctctgtca cgcgcaaaca aaaggctttt tcctgttaag cttgtttggt
    14041 tctgtaaata taatcgatta agcgcttcga tccccttatt tctttctcca tttttaactc
    14101 tttgatatga aatcacgctt acacctttgt gctttccggt ttcttctgcg cctttatagg
    14161 aatctccgct ttgaaatgaa acctgatgca atcccaaatt agttatgtgc atttaattat
    14221 cctcagtcat aaatggggca ttgattgcag ttttgtacct cgttttacaa caaaaaagat
    14281 acttattcaa cagggttctc tctttttcta taacactccg agcctgatac atcaggcact
    14341 ttatcttgtg ggatttcatg atgaaacgag cactacatat gctcacgagg tacatattat
    14401 gtacagcaaa agaaaatttg attatgttaa caggctcaag tttctaaatt tattaattga
    14461 atatattata taccaaatac attttacatc cccacaatca ccaagtaatg gagagttaat
    14521 aaagtatgat cctcaaaatt agcaatttca ttgacgcaag caatacaaaa gggcctatac
    14581 gcgttgaaga tacagagcat ggacctatat tgatagcaca gaaattcaac ctaaaggatc
    14641 tctttttcag aacattaagc actattaatg caaagataaa ttcacagata ttaaatgaac
    14701 aactaaaaaa ttatcgacta gagaatcaaa aatccttatt gcttttcctc aatacattag
    14761 ctagcgaaaa atctgcagaa agtgcgtttg ccgcctatga agcagctaaa aactcaattc
    14821 aacactcttt cacaggtaga gacatcaaat taatgttgaa taccgcagag cgttttcatg
    14881 gcatcggtac agccaaaaat cttgaaagac atttagtttt tcgttgctgg ggaaatagag
    14941 gcataaccca tttagggcat actagtatca gtataaaaaa caatttacta caggaaccaa
    15001 ctcataccta tctcagctgg tatcccggcg ggaatgttac aaaagataca gaaataaact
    15061 acctttttga aaaacgctca ggttacagtg tcgataccta taagcaagac aaactaaata
    15121 tgatttcaga ccaaacagcc gaaagacttg atgcaggcca agaggttaga aacttattaa
    15181 attcaaaaca agatcaaaat aacaacaaaa aaatattttt tcccagagct aatcaaaaaa
    15241 aagaccccta tggttattgg ggggtcagtg ctgataaagt atacattcca ttatcaggtg
    15301 ataataaaac aaaggatggt aaaatcagcc ataacctttt tggccttgat gaaacaaaca
    15361 tgtcaaaatt tatatgcaaa aagaaagcag atgctttcag acaattggca aattataaat
    15421 taatcagtaa atctgaaaac tgtgctggta tggcacttaa tgttcttaaa gcaggaaatt
    15481 ctgaaatata ctttccactc cctgacgtta aattggttgc tacacctaac gatgtatacg
    15541 cgtatgcaaa taaagttagg caacgcattg aaagtctcaa tcagtcttat aacgaaataa
    15601 tgaagtatat tgaatctgat tttgatcttt ccagattgac tcaattacga cgcagctatc
    15661 ttaaaagttt caataaaatt aaccttatcc acacaccaaa aacattcaag cctttatcaa
    15721 tttcacttta caaacatcct actgaaaatg tatcttcaga agactttgat gcggtcatca
    15781 acgcttgcca ctcatactta gtaaaatcag cacccagcaa tatgactcgt gtattaaacg
    15841 aattaaaaac cgaagcaaca gataaaaaag aagaaatcat cgaaaaatca ataaaaatta
    15901 ttgattatta caattctctt aaatcgccag atcttggaac caaactttac attcacgact
    15961 tattacagat caataagctt ttattgaata actctcattc taatatatag aaggccatag
    16021 aaatgtgtcg caaactatat gataaacttt atgaaataac aggtgcaaag cttgatttta
    16081 acgataaaaa tcaagccttt atattgctcg aagaacaaat acctgtttgt ataacagata
    16141 atgatgaata catctttttg accggacttc ttaatgaaca tgaacttttt acagaaaata
    16201 ttattaatcc agaacacata ctcatcctta actactcttt gagtagagat tatggttctt
    16261 ctatctgtct tctccctgat actcatcaat gtgttttgac caagaaacac tacaaaaaat
    16321 atctttcacc tgatgaactc attgaatctt tgtatgaatt tttattttgt attaaactaa
    16381 ccattgcaaa catcacttca gaagtgaact aacatatagg gggtatcatg caaattctaa
    16441 acaaaatact tccacaggtt gaattcgcga ttcctcgccc atcatttgat tcgttaagtc
    16501 gcaataagct tgtaaagaaa atattatctg tttttaactt aaaacaacgc tttcctcaga
    16561 agaattttgg atgcccggtg aatataaata aaatcaggga cagcgtcata gacaaaataa
    16621 aagatagtaa ttcggggaat caactatttt gttggatgag ccaagagcga accacttatg
    16681 tctcttcaat gataaacaga agtatagatg aaatggccat acacaatgga gttgttttga
    16741 cctcagataa caaaaaaaac atatttgcag ctattgaaaa aaaattcccg gatataaaac
    16801 ttgatgaaaa atcagcacaa acatctatca gtcatacagc actaaacgag attgcctcat
    16861 ctggcctcag agcaaagatt ttaaaacgct atagcagtga catggatttg tttaacacac
    16921 aaatgaagga tcttacaaat ctagtatcca gttccgtcta cgataaaata tttaatgaat
    16981 caacaaaagt tttacaaata gaaataagcg cagaggttct aaaggctgta taccgtcaaa
    17041 gcaatacaaa ttaaatcttt tatattaaat cttatactta ttaaagtaaa gcagacaaaa
    17101 aggagacctt atgtctttaa atatcaccga aaatgaaagc atctctactg cagtaattga
    17161 tgcaattaac tctggcgcta cactgaaaga tattaatgca attcctgatg atatgatgga
    17221 tgacatttat tcatatgctt atgactttta caacaaagga agaatagagg aagctgaagt
    17281 tttcttcagg tttttatgta tatacgactt ttacaatgta gactacatta tgggactcgc
    17341 agctatttat cagataaaag aacagttcca acaagcagca gacctttatg ctgtcgcttt
    17401 tgcattagga aaaaatgact atacaccagt attccatact ggacaatgcc agcttcggtt
    17461 gaaagccccc ttaaaagcta aagagtgctt cgaactcgta attcaacaca gcaatgatga
    17521 aaaattaaaa ataaaagcac aatcatactt ggacgcaatt caggatatca aggagtaatt
    17581 attatgcata atgtaagcac cacaaccact ggttttcctc ttgccaaaat attggcttcc
    17641 actgagcttg gagacaatac tatccaagct gcaaatgatg cagctaacaa attattttct
    17701 cttacaattg ctgatcttac tgctaaccaa aatattaata caactaatgc acactcaact
    17761 tcaaatatat taatccctga acttaaagca ccaaagtcat taaatgcaag ttcccaacta
    17821 acgcttttaa ttggaaacct tattcaaata ctcggtgaaa aatctttaac tgcattaaca
    17881 aataaaatta ctgcttggaa gtcccagcaa caggcaagac agcaaaaaaa cctagaattc
    17941 tccgataaaa ttaacactct tctatctgaa actgaaggac taaccagaga ctatgaaaaa
    18001 caaattaata aactaaaaaa cgcagattct aaaataaaag acctagaaaa taaaattaac
    18061 caaattcaaa caagattatc cgaactcgac ccagagtcac cagaaaagaa aaaattaagc
    18121 cgggaagaaa tacaactcac tatcaaaaaa gacgcagcag ttaaagacag gacattgatt
    18181 gagcagaaaa ccctgtcaat tcatagcaaa cttacagata aatcaatgca actcgaaaaa
    18241 gaaatagact ctttttctgc attttcaaac acagcatctg ctgaacagct atcaacccag
    18301 cagaaatcat taaccggact tgccagtgtt actcaattga tggcaacctt tattcaacta
    18361 gttggaaaaa ataatgaaga atctttaaaa aatgatctgg ctctattcca gtctctccaa
    18421 gaatcaagaa aaactgaaat ggagagaaaa tctgatgagt atgctgctga agtacgtaaa
    18481 gcagaagaac tcaacagagt aatgggttgt gttgggaaaa tacttggggc acttttaact
    18541 atcgttagtg ttgttgcagc agctttttct ggaggagcct ctctagcact ggcagctgtt
    18601 ggtttagctc ttatggttac ggatgctata gtacaagcag cgaccggcaa ttccttcatg
    18661 gaacaagccc tgaatccgat catgaaagca gtcattgaac ccttaatcaa actcctttca
    18721 gatgcattta caaaaatgct cgaaggcttg ggcgtcgact cgaaaaaagc caaaatgatt
    18781 ggctctattc tgggggcaat cgcaggcgct cttgtcctag ttgcagcagt cgttctcgta
    18841 gccactgttg gtaaacaggc agcagcaaaa cttgcagaaa atattggcaa aataataggt
    18901 aaaaccctca cagaccttat accaaagttt ctcaagaatt tttcttctca actggacgat
    18961 ttaatcacta atgctgttgc cagattaaat aaatttcttg gtgcagcggg tgatgaagta
    19021 atatccaaac aaattatttc cacccattta aaccaagcag ttttattagg agaaagtgtt
    19081 aactctgcca cacaagcggg aggaagtgtc gcttctgctg ttttccagaa cagcgcgtcg
    19141 acaaatctag cagacctgac attatcgaaa tatcaagttg aacaactgtc aaaatatatc
    19201 agtgaagcaa tagaaaaatt cggccaattg caggaagtaa ttgcagatct attagcctca
    19261 atgtccaact ctcaggctaa tagaactgat gttgcaaaag caattttgca acaaactact
    19321 gcttgataca aataaggaga atgttatgga aattcaaaac acaaaaccaa cccagatttt
    19381 atatacagat atatccacaa aacaaactca aagttcttcc gaaacacaaa aatcacaaaa
    19441 ttatcagcag attgcagcgc atattccact taatgtcggt aaaaatcccg tattaacaac
    19501 cacattaaat gatgatcaac ttttaaagtt atcagagcag gttcagcatg attcagaaat
    19561 cattgctcgc cttactgaca aaaagatgaa agatctttca gagatgagtc acacccttac
    19621 tccagagaac actctggata tttccagtct ttcttctaat gctgtttctt taattattag
    19681 tgtagccgtt ctactttctg ctctccgcac tgcagaaact aaattgggct ctcaattgtc
    19741 attgattgcg ttcgatgcta caaaatcagc tgcagagaac attgttcggc aaggcctggc
    19801 agccctatca tcaagcatta ctggagcagt cacacaagta ggtataacgg gtatcggtgc
    19861 caaaaaaacg cattcaggga ttagcgacca aaaaggagcc ttaagaaaga accttgccac
    19921 tgctcaatct cttgaaaaag agcttgcagg ttctaaatta gggttaaata aacaaataga
    19981 tacaaatatc acctcaccac aaactaactc tagcacaaaa tttttaggta aaaataaact
    20041 ggcgccagat aatatatccc tgtcaactga acataaaact tctcttagtt ctcccgatat
    20101 ttctttgcag gataaaattg acacccagag aagaacttac gagctcaata ccctttctgc
    20161 gcagcaaaaa caaaacattg gccgtgcaac aatggaaaca tcagccgttg ctggtaatat
    20221 atccacatca ggagggcgtt atgcatctgc tcttgaagaa gaagaacaac taatcagtca
    20281 ggccagcagt aaacaagcag aggaagcatc ccaagtatct aaagaagcat cccaagcgac
    20341 aaatcaatta atacaaaaat tattgaatat aattgacagc atcaaccaat caaagaattc
    20401 gacagccagt cagattgctg gtaacattcg agcttaatat atccaagagc cataataata
    20461 tatggctctt cctgtaagga aataaccatg aatataacaa ctctgactaa tagtatttcc
    20521 acctcatcat tcagtccaaa caataccaac ggttcatcaa ccgaaacagt taattctgat
    20581 ataaaaacaa cgaccagttc tcatcctgta agttccctta ctatgctcaa cgacaccctt
    20641 cataatatca gaacaacaaa tcaggcatta aagaaagagc tttcacaaaa aacgttgact
    20701 aaaacatcgc tagaagaaat agcattacat tcatctcaga ttagcatgga tgtaaataaa
    20761 tccgctcaac tattagatat tctttccagg cacgaatatc caattaataa agacgcaaga
    20821 gaattattac attcagctcc gaaagaagcc gagcttgatg gagatcaaat gatatctcat
    20881 agagaactgt gggctaaaat tgcaaactcc atcaatgata ttaatgaaca gtatctgaaa
    20941 gtatatgaac atgccgttag ttcatatact caaatgtatc aagattttag cgctgttctt
    21001 tccagtcttg ccggctggat ctctcccgga ggtaacgacg gaaactccgt gaaattacaa
    21061 gtcaactcgc ttaaaaaggc attggaagaa ctcaaggaaa aatataaaga taaaccgcta
    21121 tatccagcaa ataatactgt tagtcaggaa caagcaaata aatggcttac agaattaggt
    21181 ggaacaatcg gcaaggtatc tcaaaaaaac gggggatatg ttgtcagtat aaacatgacc
    21241 ccaatagaca atatgttaaa aagcttagat aatctaggtg gaaatggcga ggttgtgcta
    21301 gataatgcaa aatatcaggc atggaatgcc ggattctctg ccgaagatga aacaatgaaa
    21361 aataatcttc aaactttagt tcaaaaatac agtaatgcca atagtatttt tgataattta
    21421 gtaaaggttt tgagtagtac aataagctca tgtacagata cagataaact ttttctccat
    21481 ttctgaggtg cgccatgcat aatgtaaata atactcaagc gccaacattc ttatataagg
    21541 caacttcacc atcatcaaca gaatacagcg agttaaaaag caaaatatcc gatatccata
    21601 gttcgcaaac ttctctaaaa acaccagcat cagtgtctga aaaagaaaac tttgcaacgt
    21661 cttttaatca gaaatgtctt gattttttat tttcttcctc agggaaagaa gatgtgttaa
    21721 gaagcattta ttccaactca atgaatgcgt atgccaaaag cgagattctc gaattttcaa
    21781 atgttttgta ctccttagta catcaaaatg gtcttaattt tgaaaacgaa aagggacttc
    21841 aaaaaattgt cgcacagtat tcggaactaa ttataaaaga taaattatcc caagattctg
    21901 cctttggacc atggtcggca aagaataaga aactccatca attacgacaa aacattgagc
    21961 acagacttgc actattagca caacaacaca catctggtga agctttatca ttgggacaaa
    22021 aactcctcaa tactgaagta tcatcattta tcaagaataa tattcttgct gaattaaagt
    22081 taagtaatga aactgtttca tctctcaaac tagatgattt agttgacgca caggcaaaac
    22141 ttgcctttga tagtttgcgc aatcaacgta aaaatactat tgatagtaaa ggatttggta
    22201 taggtaaact gtcaagagac ttaaatacag tagccgtgtt tcctgagctg ttgagaaaag
    22261 tccttaatga tattttagaa gatataaaag attcgcatcc tatccaagat ggcctcccta
    22321 cacctcccga agatatgcca gatggcggac caacccccgg agccaatgag aaaacatccc
    22381 aacctgtaat tcactatcat ataaataatg ataatagaac ttacgataat agagtttttg
    22441 acaacagagt atatgacaat agctatcacg agaacccaga aaatgatgca cagtctccta
    22501 cttctcagac aaacgatcta ttatcccgta acggaaactc attactaaat ccacaaagag
    22561 cactagttca aaaagtaact tccgttctac cacactctat atcagatact gtccagacat
    22621 ttgcaaataa ttcagcttta gaaaaggctt tcaaccatac tccagataat tcggatggaa
    22681 taggttcaga cctgttaact acgagtagtc aagaaagatc tgcaaataac tctctttctc
    22741 ggggacacag gcctctgaac atacagaact cttcaaccac cccccctctc cacccggaag
    22801 gagtgacaag cagtaatgat aactcatcag atacaactaa aagtagcgct tctctttctc
    22861 atagagtagc ttcgcaaatc aataaattca actcaaacac tgattcaaaa gtacttcaga
    22921 ctgatttttt atcaagaaat ggagacacat atttaacacg ggaaacgata tttgaagctt
    22981 caaaaaaagt aacaaactcc ctaagtaatc ttatatctct cattggaact aaatcaggaa
    23041 cacaagaacg agagttacag gaaaaatcaa aggacattac aaaatccaca acagaacata
    23101 gaataaacaa caaattaaaa gttacagatg caaatataag aaactacgta acagaaacca
    23161 acgcagatac aattgataaa aatcatgcga tctatgaaaa ggcaaaagaa gtatctagcg
    23221 ccctcagcaa ggtattgtca aaaattgacg atacctctgc agaattactt acagatgata
    23281 tatctgattt aaaaaataac aatgatatta cagctgaaaa caataatata tataaagcag
    23341 caaaagatgt aaccacttcc ctatcaaaag tattaaagaa tatcaataag gattaaaata
    23401 tgataaaaga aaaaatatta tcaatagtgg ccttttgcta tggtatagcg tacagcaaac
    23461 tatctgaaga aactaaattt attgaagatc tttctgccga ttctctttct ctgattgaaa
    23521 tgctggatat gattagtttt gagttcaatc tacgtataga tgaatctaca ttagaacaca
    23581 ttattactat tggagatctt atttctgtag tcaaaaatag tacaaaatca atatagtaat
    23641 tgataagcat tttttcatct atggagctct cacatcagag ctccacaaga atattattct
    23701 tttatccaat aaagataaat tgcatcaatc cagctattaa aatagtaatt tttaagacta
    23761 ccgttgacta tcatcaacag ttacatacaa tcttgctcac actgcattta acttttgtca
    23821 atataaatca atatgaataa acagggtgtg atatggtgga tttgtgcaac gacttgttaa
    23881 gtataaagga aggccaaaag aaagagttta cactccattc tggtaataaa gtttccttta
    23941 tcaaagccaa gattcctcat aaaaggatcc aagatttaac cttcgtcaac caaaaaacga
    24001 atgtacgcga tcaagaatcc ctaacagaag aatcattagc cgatatcata aaaactataa
    24061 agctacaaca attcttccct gtaataggaa gggagattga tggtagaatt gaaattctgg
    24121 atggcactcg tagaagagca tctgcaatat atgcaggagc agatcttgaa gttctatatt
    24181 caaaagaata tatatctact cttgatgcca gaaaactagc aaacgatata caaacagcaa
    24241 aagagcatag catccgagaa cttggtattg gtcttaattt tctgaaagta tcagggatgt
    24301 cctataaaga catagccaaa aaagagaatc tgtctcgcgc gaaagtcact cgtgcctttc
    24361 aggcagcaag cgttccacag gaaataatat ctctatttcc aatcgcgtca gaacttaact
    24421 ttaatgacta caagatatta ttcaattatt ataaaggact tgaaaaggct aatgaatctc
    24481 ttagttctac actaccaata ttaaaggaag aaataaaaga ccttgataca aatttgcccc
    24541 cggacatata taaaaaagaa attttaaaca tcataaagaa aagcaaaaac agaaagcaaa
    24601 acccttcgct aaaagttgac tcgttattta tttctaaaga caaacggact tacataaaaa
    24661 gaaaagagaa taaaacaaac agaactctaa tatttacatt atctaaaata aacaaaacag
    24721 ttcagagaga aatagatgaa gctattcggg atataatttc tcgccatcta tcgtcttcat
    24781 aaacatcgaa cactgatgta aactgcccaa ttcagataat aaaaatcttc gttgaacttg
    24841 actttcagcc ggtggggtaa ttccgtcggc tatctgttat tcaatcccca cgcactgtgt
    24901 ttccttaaaa ttacggtgaa aactcatacc ctgctgacca cgagaaaatt atctggtgaa
    24961 attagtaata ttcatatcat tgaaaatgaa ttgtgcaact aagggatgcg gcatgatggt
    25021 caaaatttta tgtgaatatg agtgactggc cgaaaatatt cagcgtattt ttaatcgttt
    25081 caataaaaac agcgaggatt agcagtcaac gcttttacta ctcaattttt tttacatggt
    25141 gataaacaaa aaatccacca ttaacagact tcgttcacag tatcaaaaaa gctttaagaa
    25201 tatattagca gatttctcaa atataattac tgttttattc tcctaatctt cccataaaat
    25261 gaccgactta cattatttta taattataaa caagtttgat tttaaaatca tattaattat
    25321 taacaagaga tttatttttc taaccccaaa agttatgctt ggtcaaagag taacttataa
    25381 tatccatacc cctcataggg cttacagttt aaattgcggg tataaaatac atctattttc
    25441 aaataaaata aagcatactc caattacaac gagtgactaa aataaaaata tcatccaagt
    25501 caatcactgt ttgctctgca aaatatgtca gccttctcaa acttaccagc acttaaatat
    25561 ttaagcacac aatcattcct tgagggagtt cacttatttc aagagaaaca tttcgtcttc
    25621 ccacacaact aatccgccca atatagctaa atttgtactc agtaatatca tttgttatgc
    25681 cccccacccc aaaatattca agctccttca cagataaaaa acgacaattt acaaagcctc
    25741 attagttata actatggaaa tacctatttt agtatatcct ataaagttag aattacttct
    25801 tcttgcacct aattccatag ttgaaaaaca agaataattt tctcctacag ctggatccat
    25861 atagcttcca tcaggtctac acaacaccca gtggaggccg acaggaactc caataaagga
    25921 tacagcaaca gcttccaaaa ctctttggtt agaagataac acatctcgtt gatgtactat
    25981 attacatccc atgccaatta ataagtccct tgcatcggga taaataaaac taattacttg
    26041 cgaaaaaatg ttactttctt caacgacata agcattcaat ccaagaagcc tcgtagccat
    26101 gactatctta tcaggcatag aataaccggc atccgcgata ttatctttgt gtattctcgg
    26161 attataatta ccactagtta ttaagtataa atcccgctca caacgattat ccaaatcaag
    26221 agaactcttc cttgtcattt cggacataga ccctttgtat tgtggtattt tatcaacccc
    26281 taattccttt gcagcacaga gtaaactggc agctccacaa gaattgtcat acatctgctt
    26341 cctcgccaaa aaacaacctt gttcgtttac tctattctca tttaccctca catcagtctg
    26401 atgagatatc atatattctc ttggtatagc cccctgcaat aaaaccccat ataacataac
    26461 acctgtatga atacatcccc gtttgcaagg cttccgttgt tccgacatcg acgagtaatt
    26521 gcagcacagt attgcatcca gcaatgcgaa ctactatgag aattagctga ccgggtcctt
    26581 attccccgaa cgaactttaa atccaaaacc tcatactctt ttccgtcgtc atcacgtccg
    26641 tcaccataac aacctttcag catccaccct tttattaaga gttaatgtcc atcagcatca
    26701 gcaattaata ccgcctgata ctgacatatt tttgtcgtat ttacgtccat aatcggttat
    26761 tgtgatacag gtcacaccag cttatttgct attgttacaa taaccacctt ggttgagagc
    26821 tgttttttca actttgttat caactccccc aactccttgg tcagtaaggc tgccgaccga
    26881 gcacagtgaa tagatagtat ccgcaggtac caccatgctt acttaatact cagaaggcga
    26941 agtgctctcc aattaactct gccgcactat gcttataagc tgcttttcaa tgccgtctat
    27001 caacatgtcc aattcattca atctgttgta ctggtaatcc aacgcagtaa tgatatatgc
    27061 aggtattctt tcatccactc cagtaacgtt tcatgcaaag cattaattta cgcgctacgg
    27121 aacttcacca gttgactgag caaacgatgt gggaccagta ctgcctgcag ttcttctatt
    27181 gttacagcga tttctttacc aaacagctga accaccatca agataaagat agcctgaaca
    27241 ttcatcgcac cattttgtta ccaatggcaa aagatttaac gaaccgtgcc tgcatcaggc
    27301 ggacttttta tccctgcttt gttagctccc taaccttgta cagagagcct ccataagctc
    27361 ccatatcaag cagacatggt tcgcagttac tgacatacac cagaaaactc tgacagcaga
    27421 ctcacttatc ctccacttca ctcatatcct tattggtgag atgaacctga atgacatgtt
    27481 ttgcgacata aacgccaacc agtgtatatt tcacctgtag agattctaca atctgtagcg
    27541 ttatgcatct aatgttaggc tctatgatgt cagaaatctg cgagactcta caccttctgc
    27601 atttaccttt atttatcacc atgctcctca gttaacggag aacgtgttca ttgcattttg
    27661 tcacttacat ttcgcctttg gtatcggggg gatataggtt ccaacgttcc gcatgagaac
    27721 ctgaaccggc ttcacgccac cttgatacca gatgccgcac aggtagtcaa caagtgtcac
    27781 gaacggtgca atagtgatcc acacccaacg cctgaaatca gatccagggg gtaatctgct
    27841 ctcctgattc aggagagctt atggtcactt ttgagacagt tatggaaatt aaaatcctgc
    27901 acaagcaggg aatgagtagc cggacgattg ccagagaact ggggctctcc cgcaataccg
    27961 ttaaacgtta tttgcaggca aaatctgagc cgccaaaata tacgccgcga cctgctgttg
    28021 cttcactcct ggatgaatac cgggattata ttcgtcaacg catcgccgat gctcatcctt
    28081 acaaaatccc ggcaacggta atcgctcgtg agatcagaga ccagggatat cgtggcggaa
    28141 tgaccattct cagggcattc attcgttctc tctcggttcc tcaggagcag gag
//
LOCUS       DAJTRH010000050        27960 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTRH010000050 DAJTRH010000000
VERSION     DAJTRH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            564..1334
                     /locus_tag="OO339_002280"
     CDS             564..1334
                     /locus_tag="OO339_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5926894.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO339_002281"
     CDS             1325..1798
                     /locus_tag="OO339_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5926895.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO339_002282"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO339_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5926896.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO339_002283"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO339_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5926897.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO339_002284"
     CDS             3233..3727
                     /locus_tag="OO339_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5926898.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3967..6258
                     /gene="opgB"
                     /locus_tag="OO339_002285"
     CDS             3967..6258
                     /gene="opgB"
                     /locus_tag="OO339_002285"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5926899.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO339_002286"
                     /pseudo
     CDS             complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO339_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7557..8471
                     /locus_tag="OO339_002287"
     CDS             7557..8471
                     /locus_tag="OO339_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5926900.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8686..10047
                     /locus_tag="OO339_002288"
     CDS             8686..10047
                     /locus_tag="OO339_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5926901.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO339_002289"
     CDS             complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO339_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5926902.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO339_002290"
     CDS             complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO339_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5926903.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12597..13886
                     /locus_tag="OO339_002291"
     CDS             12597..13886
                     /locus_tag="OO339_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5926904.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13883..15349
                     /gene="hpaE"
                     /locus_tag="OO339_002292"
     CDS             13883..15349
                     /gene="hpaE"
                     /locus_tag="OO339_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5926905.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15351..16202
                     /gene="hpaD"
                     /locus_tag="OO339_002293"
     CDS             15351..16202
                     /gene="hpaD"
                     /locus_tag="OO339_002293"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5926906.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16212..16592
                     /locus_tag="OO339_002294"
     CDS             16212..16592
                     /locus_tag="OO339_002294"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5926907.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16660..17463
                     /gene="hpaH"
                     /locus_tag="OO339_002295"
     CDS             16660..17463
                     /gene="hpaH"
                     /locus_tag="OO339_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5926908.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17474..18277
                     /gene="hpaI"
                     /locus_tag="OO339_002296"
     CDS             17474..18277
                     /gene="hpaI"
                     /locus_tag="OO339_002296"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5926909.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18299..19654
                     /gene="hpaX"
                     /locus_tag="OO339_002297"
     CDS             18299..19654
                     /gene="hpaX"
                     /locus_tag="OO339_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5926910.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19664..20554
                     /gene="hpaA"
                     /locus_tag="OO339_002298"
     CDS             19664..20554
                     /gene="hpaA"
                     /locus_tag="OO339_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5926911.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20581..21518
                     /locus_tag="OO339_002299"
                     /pseudo
     CDS             20581..21518
                     /locus_tag="OO339_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21745..23303
                     /gene="hpaB"
                     /locus_tag="OO339_002300"
                     /pseudo
     CDS             21745..23303
                     /gene="hpaB"
                     /locus_tag="OO339_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23321..23833
                     /locus_tag="OO339_002301"
     CDS             23321..23833
                     /locus_tag="OO339_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5926912.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24226..26376
                     /gene="btsT"
                     /locus_tag="OO339_002302"
     CDS             24226..26376
                     /gene="btsT"
                     /locus_tag="OO339_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5926913.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26426..26629
                     /locus_tag="OO339_002303"
     CDS             26426..26629
                     /locus_tag="OO339_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5926914.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26640..27596
                     /gene="yjiA"
                     /locus_tag="OO339_002304"
     CDS             26640..27596
                     /gene="yjiA"
                     /locus_tag="OO339_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5926915.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27665..>27960)
                     /locus_tag="OO339_002305"
     CDS             complement(27665..>27960)
                     /locus_tag="OO339_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5926916.1"
                     /translation="HILPQALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQH
                     YDFIKKKSIYEKLNSKSSFYLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6812 a         7333 c         7061 g         6754 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaatgtt acccggctga cgcggctctc tcctgccctt ttccctgctc
     3781 ctggacggtt ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa
     3841 actaattcct aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc
     3901 attacattgt gcttctcaaa aagcttaaac gttggctaaa cggcccggtt tagtgaggat
     3961 cgcccattgt cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca
     4021 tggaaagcgg gacgtaacac ctggtggttt gcagccacgt taacggtgct ggggctattt
     4081 gtcgttttaa atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac
     4141 gcggttctct ataccttaac caacagcctg accggtgctg gcgtcagcaa atacatcctg
     4201 ccgggtatcg gcattgtgct ggggctgacg gcggtgttcg gtgcgctggg ctggatcctg
     4261 cgccgtcgtc gtcatcatcc gcaccatttt ggttacagcc tgctggcgct cttactggcg
     4321 ctgggttcgg tggacgccag cccggcattt cgtcagataa cggaactggt gaaatcccag
     4381 tcacgcgatg gcgacccgga ctttgcggct tattataaag agccgtcgaa aactatccct
     4441 gacccgaaac tcaacctggt ttatatctac ggcgaaagtc tcgagcggac ctattttgat
     4501 aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat
     4561 ttcagccaca cgcagcagct gccaggaacg gattacacga ttgcgggcat ggtggcttct
     4621 cagtgcggca taccgctgtt cgcccccttt gaaggcaacg cctccgcctc tgtctccagc
     4681 ttcttcccgc agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat
     4741 ttcgtgcagg gcgcaaatct gcgttttgcc ggtaaagatg tgttcctgaa atcgcacggc
     4801 ttcgaccact tatacggctc agaagagctg aaaagcgtgg tagccgaccc gcactatcgc
     4861 aacgactggg gattctacga cgataccgtt ctcgatgaag cgtggaaaaa gtttgaagag
     4921 ctttcccgct caggtcagcg attctcactg tttaccctga cagtcgatac ccatcacccg
     4981 gatggtttta tctcgcgcac ctgtaaccgc aaaaaatatg attttgacgg taagccgaat
     5041 cagtcattca gcgcggtaag ttgcagccag gagaatatcg cgacgtttat caacaaaatc
     5101 aaagcgtcac cgtggtttaa agatactgtc atcgtcgtct cttctgacca tttagcgatg
     5161 aacaacacgg cgtggaaata cctcaataag caggatcgca ataacctgtt ttttgtcatt
     5221 cgtggcgaca agccgcagca aaagacgctg gcagtgaagc gtaacacgat ggataacggc
     5281 gcgacggtgc tggacattct cggtggcgat aactatctcg gacttggtcg tagcagttta
     5341 tccgggcagt cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag
     5401 ccagatatca tccgcctgtg gaaattccct aaagagatga aagagttcac catcgaccag
     5461 cagaaaaaca tgattgcctt ctcgggtagc catttccgtt tgccgctgct gttgcgggtt
     5521 tcagacaaac gcgtggaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag
     5581 ctggccgatt tcgctccacg cgacaatttc gtctgggttg acagttgcta caagatggca
     5641 caactctggg ctccggaact ggcactttcc accgactggt gtgtctcgca ggggcagctt
     5701 ggcggtcagc aaattgttca gcatgttgac aaaacaacat ggaagagcaa gacggcattt
     5761 aaagatacgg tcatcgacat ggcgcgttac aaaggcaatg tcgatacgct gaagattgtt
     5821 gataacgata ttcgctacaa agccgacagt ttcatcttta acgtcgccgg tgcgccagaa
     5881 gaggtgaaac agtttagcgg gatttcccgt ccggagtcgt ggggccgctg gtccaacgcg
     5941 cagctgggcg atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg
     6001 gtgattaccg ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc
     6061 aatgaagaac aaactcttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat
     6121 aacccaaccg atgccgacac actggtaatt gtgccgccgg aacctgtctc aaccaacgaa
     6181 gggaatatcc tcggacactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtg
     6241 gtagaacgtg aagggtaatt cctgactgat gtgtaggtcg catagggtgc gacctacacg
     6301 ccttctccgg cttacaacac attattaaga aacaaaaaaa acagcgccca acacttcccc
     6361 ggacgctgca tggcaacttt aataatctcg ccttatcaga aagtaattac acccttaatc
     6421 aactcacgat tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag
     6481 cgatgggtta acatcatgtc ggcagtgatt ttcccttccg ccatcagtcg accgacttta
     6541 gcgaaatctt ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat
     6601 tccggatcgg agaactgcaa ctcaccttta aacagaccga caaataccac cgtgccgccg
     6661 tgacgaatca gattcacggt attattcatc gcatgttgat tacctgtcgc gtcgatcact
     6721 ttctgcgcca gcgaaccacc aaactgcgcc cgcagctgcg cgtcaaaatc ttcggctgac
     6781 gggtccagca cgggcaactc cagacgcgtt gccacatgtt cacggcgcgc cggactggta
     6841 tccgccacca ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt
     6901 ggccccgccc cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca
     6961 tgagcgctaa tagcgaaagg ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg
     7021 ggcaaaatgt tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg
     7081 ccaatgactg aaattttttc acagcagttg gtacgcccgc ttttacacgc cgggcattgc
     7141 tgacaggcaa cataagggat cacagcaact tgctgaccat ttttaagatc agcaatattt
     7201 ttacccagcc caacaatctc cccacatatt tcatggccta aaacccgtgg ataactaaaa
     7261 aatggttgat ttccacccta ggcatgaata tcggtaccgc aaatcccgac agacttaatt
     7321 tttattaatg cttcattgtc acccggaata ggtatctcgc gttgtttcca gactaattct
     7381 ttcggctgct ggcaaattaa aacattcatc gtagacatga ttcggctatc cattttttgt
     7441 tgccttagat atcaatgaaa atgaaaaatg gcagcggaac ggaaaatctg ttttgtgaaa
     7501 acacacgcat aaaaatgttt taaatcgggt tttaatggac tcacaaggag gaaaatatga
     7561 gtcgttcaca aaatttacgc cacaatgtga ttaaccaggt cattgatgat atggcgcggg
     7621 gtcatatccc ttccccactt ccatcgcaaa gtgcgctggc ggaaatgtac aacatcagcc
     7681 gcactacagt gcgtcatatt ctcagccaat tacgcgaatg cggcgtctta acgcaggttg
     7741 gaaacgacta tgttattgcg cgtaaacctg accatgatga cggtttcgcc tgtactaccg
     7801 cttcaatgag tgaacaaaac aaagtgtttg agcaggcttt tttcacgatg ataaaccagc
     7861 gtcagttacg ccctagggaa actttttctg aactgcaact ggctcgggcc gcgggtgtca
     7921 gcccggtggt tgtaagagaa tatcttttaa aattcggccg ttataatctt attcaaagcg
     7981 aaaagcgcgg ccagtggagc atgaagcaat tcgatcaatc ctacgccgag cagctgtttg
     8041 agctacgcga aatgctcgaa acacattcac ttcagcactt tctcaacctg cctgatcatg
     8101 atccccgctg gctgcaggcg aaaaccatgc tagaacgtca tcggctactg cgtgacaaca
     8161 tcggcaatag ctttcgcatg ttctcgcaac ttgacagaga ttttcactcc ttactgcttt
     8221 cagctgccga caatatcttt tttgatcaat cgcttgagat catctcagtc atctttcatt
     8281 ttcactatca atgggacgaa agcgatctta aacagcgcaa tatcattgcc gtcgatgaac
     8341 atatgaccat tctcagcgct ctgatttgcc gcagtgacct tgatgccacc cttgcgctcc
     8401 gtaaccattt gaattcagct aaacaatcaa tgattcgatc cattaatgag aacacgcgtt
     8461 atgctcatta agtacggatt aaaaacagca aactattagc gggtgcacgg ttttatcaaa
     8521 agataaatac cgggaaattc ccgcttacct atgctcacaa tcagtcgact aaaaatgttt
     8581 ttgtgagcgg tagtaaagtc ctaaaacttt aacctgccgt taatgtcaac cttgcgtaat
     8641 gcacaattac gtaaacaatt aaaattataa atacaggagt ccggcgtgga aaaagaaaat
     8701 atcaccctcg atccgcgttc ttcatttact ccatcttcgt cggcagatat tcccgtgcca
     8761 ccagatggat tagttcaacg cagtacccga attaaacgca ttcaaaccac cgccatgttg
     8821 ttattatttt ttgcggcggt aatcaattat ctcgaccgca gttcgctgtc ggtagcaaat
     8881 ttaacgattc gtgaagaatt gggattaagt gccaccgaaa tcggcgcttt gctctccgtg
     8941 ttttcactcg cttacggcat tgcgcaactt ccttgcggcc cactattgga tcgtaaaggc
     9001 ccacgcctga tgctgggact ggggatgttc ttctggtcac tgttccaggc catgtctggc
     9061 atggtgcaca gctttacgca gttcgtgttg gtgcgtatcg gtatggggat tggtgaagcg
     9121 ccgatgaacc catgcggtgt aaaagtcatt aacgactggt tcaacatcaa agagcgcgga
     9181 cgcccgatgg gcttcttcaa cgcagcttct accattggcg ttgctgtaag cccaccgatt
     9241 ctggcggcaa tgatgctggt gatgggctgg cgcgggatgt ttattaccat tggtgtactg
     9301 gggatttttc tcgccatcgg ctggtatatg ctctatcgca accgcgagca cgtagaactg
     9361 actgccgttg aacaagctta tctcaatgca ggtagcgtca atgcccgccg agatccgctc
     9421 aattttgccg aatggcgcag cctgttccgt aaccgcacaa tgtggggaat gatgctcgga
     9481 ttcagtggca tcaactacac tgcgtggctg tatctggcct ggcttcctgg ttacctgcaa
     9541 acagcctata acctggattt aaaaagcaca gggttgatgg cggctatccc tttcctgttt
     9601 ggggctgccg ggatgctggt caacggttac gttactgact ggctggtcaa agggggaatg
     9661 gctccgatta aaagccgtaa gatctgcatt attaccggga tgttctgttc tgccgccttt
     9721 acgctgatag taccacaagc gacaacatcc atgatggcgg ttctgctgat tggcatggca
     9781 ctgttctgta ttcactttgc cggaacatcc tgctggggct tgatccacgt cgcagttgct
     9841 tctcgcatga ctgcgtcggt gggcagtatc cagaactttg ccagcttcat ctgcgcctct
     9901 ttcgcgccga tcattactgg ttttattgtt gatacaaccc actcattccg tctggcacta
     9961 atcatttgcg gttgcgtcac cgcagcggga gcactggcgt acatcttcct ggttcgccag
    10021 ccgatcaacg acccgcgtaa agattaaact ccctatgccc gataacattt tgcttatcgg
    10081 gcatttttat ggcgattaaa acgtttccca gttctccccg ctatctgcca ctgccatttt
    10141 acgcggcgta gctggcgtca cggtttttac cacagccgat gtttcacgct gctgttgctg
    10201 ctgttgctga atccggaaca ctgccacagc ttcggtcagg cgactggcct gctcttccag
    10261 cgcggcggcg gcagcggcag attcttccac cagcgcggcg ttctgttgag ttacccggtc
    10321 catctcagca accgctaagc caacctgatc gataccacgg ctctgctcat cagaagcaga
    10381 agctatttcg cccataatgt ccgtcacgcg ggtcacagca ctgacaatct ccgccattgt
    10441 ttccccggcg ctttcgacca gcgtagagcc aacatccact ttccccaccg agtcttcaat
    10501 caggctttta atttcacgaa ccgcctgggc gctacgctgg gccagattac gcacttctcc
    10561 cgcaaccacc gcaaaaccac gtccttgctc acccgcacgc gctgcttcaa ccgccgcgtt
    10621 caaagccaga atattggtct ggaaggtaat gccgtcaatt acgctgataa tatcggcgat
    10681 tttctgcgaa ctggtggaga tatcgcgcat agtctgcacc acgttatcta ccactttgcc
    10741 gccgcgttgc gccgtttcag aagcacttaa tgccagatgg ctggcctggc gcgcattctc
    10801 ggcgttctgt ttcaccgttg cggtcagttg ctccatgctg gctgccgtct cttccagcga
    10861 agcggcctgt tgctcggtgc gcgaagagag atcattattg ccggtagcga tttcgctggc
    10921 accgctatag atggcattgg ccccgttgcg cacatcaccg acggtacgca tcagctctcc
    10981 ctgcatatgg cgcaaactct ctgccagttg ccccatctca ttagagccat ccacctcaat
    11041 cggtttcacc agatcgccgc ctgcaatatg acgaatgctg tcaatcaggc gattcattgg
    11101 cgctaccagc gaggctttaa taccgaacca gacggcgaag atgaccgcca gtacgacgat
    11161 catcacgccc accagaatcc acatcgcctg gctgtaggag gcattgttat cgctgacggc
    11221 gatatcatag agccgatcgt tttgctccat gtaagccaca tactgcttct cgaaaccgtc
    11281 ctgatatccc tgggtcggct gatcaaagaa ctcgttgatt ttgcctgcac ctaacagttg
    11341 gatcagctcc gccagcgcat tgtgataaat atcgtaatta cgtttgatct ctgccgctgc
    11401 ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa tccgcccagt ttttttccgc
    11461 ctgtttcagc gaaatactgg cactctgcat cagctcagca acggttgaac cgctaccaat
    11521 attattctga tccatcatgt agcggatacc cgcgcggttg agggtgttac gcgtctgcaa
    11581 caacgcgacc cagctgccat tcagcgtggg ttgctgctgg cgaatggttt gtaaaacagt
    11641 gaaattttct ttgtcattct ttaaggcatt aaaaaacaga ccgcctgatg tcagttgtaa
    11701 aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg atcttgatac gttttaacat
    11761 ggtttctctt tcctgtggac cgatgcaaga ttttcggcct ggaaagcaaa aactttatga
    11821 aaaggtgtaa cccgccatcc atggaaaaac caggcgctgg ttatcagata ctaaaaagtt
    11881 attcattatc gcccgggatg tcttcttgtc gggaattgcc cagagcaata aattcctcta
    11941 acagatgggt taactgttgc attttctcgg cagtaaactg agcttcaatc tgccgatagg
    12001 cttcttcaat ctgtgtctga gcgcggttat acagcgcctg cccctctttg gtcagcgaga
    12061 tatagagctt ccgctgatca ttgatcggct ttaatcgcaa cactaaaccg tcgcgttcca
    12121 tccgcgtgag gatcccggtc aggcttgggc gcaaaatgca ggcgcgatac gccagatcgt
    12181 gaaaatccat tgatgggctt tccgccagaa tacgcacgat gcgccactgt tgctcagtca
    12241 aattatgccg tttaacgatt gggcgaaaat aactcatcgc cgcttctcgc gcctggagca
    12301 acgcaatggt tagtgagtcg tgcattatct ttcccctgaa aaattcaaaa tcattaatat
    12361 agaaacaatt aatcatcacg ttaccgggac tgagctgccc ccgcaattct tcattttatc
    12421 aaaattgatg atattttatt aacttactga tatttaatat tttattatct ttaaatgtaa
    12481 atagtttgtt aattagatca catttatatc acttactttt gccaaactac gcaatcggga
    12541 ataaaagtaa tcattaacat attaatgatt aagatgatcg aatccgagga gtggtaatga
    12601 aaggcactat cttcgccgta gcgttgaacc atcgcagcca acttgatgcc tggcaggaag
    12661 cgttccagca atctccctac aaagccccgc ctaaaactgc ggtctggttt attaaaccgc
    12721 gcaatacggt gattggttgc ggtgaaccga ttccctttcc acagggtgaa aaagtactga
    12781 gcggtgcgac tgttgcgctg attgtgggga aaacggcgac gaaagtacgt gaagaagatg
    12841 cggcagagta catcgccgga tatgcgctgg ataacgacgt cagcctgccg gaagagagct
    12901 tttaccgtcc ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg
    12961 tggctctcag caatgtcgat aatctgacta tctataccga gatcaacggg cgtcctgccg
    13021 atcactggaa taccgccgat ttacaacgta acgccgcaca gctgctgagc gccctgagcg
    13081 aatttgccac gctgaatcca ggcgatgcta ttctgctcgg cacgccacag gcgcgcgtgg
    13141 aaatacagcc aagcgatcgc gtgcgtgttc tcgcagaagg tttcccgccg ctggaaaatc
    13201 cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt cccaacgctg ccacacccgc
    13261 acggtacgct gtttgccctc ggcctgaact acgccgacca cgccagcgaa ctggaattta
    13321 agccaccgga agagccgctg gtgttcctga aagcgccgaa taccctcact ggcgacaacc
    13381 agacctccgt gcgcccgaac aatattgaat acatgcacta cgaagcggag ctggtggtag
    13441 ttattggcaa gcaggcgcgt aacgtcagcg aagccgatgc catggattat gtcgcgggct
    13501 acaccgtgtg taacgactac gccattcgcg actatctgga aaactactac cgccctaacc
    13561 tgcgggtcaa aagccgcgac ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg
    13621 cgatcccgga cccgcataat ctgacccttc gcaccttcgt caacggcgag ttacgccagc
    13681 aaggcaccac cgccgatctg atcttcagcg tgcccttcct gatcgcctac ttaagcgaat
    13741 ttatgaccct gaatccgggc gacatgatcg ccaccggcac accaaaaggc ttatctgacg
    13801 tggtgcctgg cgatgaagta gtggtggaag tagaaggcgt gggccgcctg gtgaaccgaa
    13861 ttgtgagtga ggaaacagcg aaatgaaaaa agtaaatcat tggatcaacg gcaaaaatgt
    13921 tgcaggtaac gactacttcc agactaccaa tccggcaacg ggtgaagtgc tggcggatgt
    13981 ggcctctggc ggtgaagcgg agatcaatca ggcggtagcg gcagcgaaag aggcgttccc
    14041 gaaatgggct gatctgccga tgaaagagcg tgcgcgcctg atgcgccgtc tgggcgatct
    14101 gatcgaccag aacgtgcctg agatcgccgc gatggaaacc gcggacacgg gcctgccgat
    14161 ccatcagacc aaaaatgtgt tgatcccacg cgcttctcac aactttgaat ttttcgcgga
    14221 agtctgccag cagatgaacg gcaagaccta tccggttgac gacaagatgc tcaactacac
    14281 gctggtgcaa ccggtaggcg tttgtgcgct ggcgtcgccg tggaacgtgc cgtttatgac
    14341 cgccacctgg aaggtcgcgc cgtgtctggc gctgggcaat accgcggtgc tgaagatgtc
    14401 ggaactctcc ccgctgaccg ccgaccgcct gggtgagctg gcgctggaag ctggtattcc
    14461 ggcgggcgtg ctaaacgtgg tacagggcta cggcgcaacc gcaggcgatg cgctggtccg
    14521 tcatcatgac gtgcgtgccg tgtcgttcac cggcggtacc gccaccgggc gcaacatcat
    14581 gaaaaacgcc gggctgaaaa aatactccat ggagctgggc ggtaaatcac cggtgctgat
    14641 ttttgaagat gccgatattg agcgtgcgct ggacgccgcc ctgttcacca tcttctcaat
    14701 caacggcgaa cgctgcaccg ccggttcgcg catctttatt cagcagagca tctatccaga
    14761 attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc gtgggcgatc cgaacgatcc
    14821 gaatacccag gttagggcgc ttatcagtca gcaacactgg gaaaaagtct ccggctatat
    14881 ccgtctcggc attgaagaag gcgcaaccct gttggcgggc ggcccggata aaccgtccga
    14941 cctgcctgcg cacctgaaag gcggcaactt cctgcgccca acggtgctgg cggacgtaga
    15001 taaccgtatg cgcgttgccc aggaagagat tttcgggccg gtcgcctgcc tgctgccgtt
    15061 taaagacgaa gccgaaggct tacgcctggc aaacgacgtg gagtacggcc tcgcgtcgta
    15121 catctggaca caggatgtca gcaaagtgtt acgcctggcg cgtggcattg aagcaggcat
    15181 ggtgttcgtc aacacccaga acgtgcgcga cctgcgccag ccatttggcg gcgtaaaagc
    15241 ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg aaatgaagaa
    15301 cgtctgcatt tccatgggcg accatccaat tccgaaatgg ggagtctgat atgggtaagt
    15361 tagcgttagc cgccaagatc acgcacgtac cgtcgatgta tctctctgaa ctaccaggaa
    15421 aaaaccacgg ttgtcgccag ggtgcgatcg acgggcataa agagatcagc aagcgttgcc
    15481 gggaaatggg cgtcgatacc attatcgttt tcgatactca ctggctggtc aacagtgctt
    15541 atcacatcaa ctgtgcagac cattttgaag gcgtctacac cagcaacgag ctgccgcatt
    15601 ttattcgcga catgacctac aactacgagg gcaacccgga gttggggcag ctaattgccg
    15661 aggaagcctt aaagctcggc gtgcgggcaa aagcgcacaa tattcccagc ctgaaactgg
    15721 aatacggcac gctggtaccg atgcgctaca tgaatgaaga caagcacttc aaagtggtct
    15781 ccatttcggc tttctgcacg gttcacgatt ttgccgacag ccgcaagctg ggcgaagcga
    15841 ttctgaaagc gatcgaacag tacgacggca ccgtggcggt ccttgccagc ggttcgttat
    15901 cgcaccgctt tattgacgat cagcgtgcgg aagaagggat gaacagctac acccgcgagt
    15961 tcgaccgcca gatggacgag cgtgtggtga agctgtggcg cgaaggccag ttcaaagagt
    16021 tttgcaatat gctgccggag tacgccgact actgctacgg cgaaggcaat atgcacgaca
    16081 cggtgatgct gctggggatg ctcggctggg ataaatacga cggcaaggtg gagtttatta
    16141 ccgagctgtt cccaagctct ggcaccggtc aggttaacgc tgttttcccg ctgcccgcgt
    16201 aaggacgttt tatgccgcac tttatcgtgg aatgtactga aaatattcgt gaagaagccc
    16261 gcttacctga gctgtttgcc agtgttaaca ctgcactggc ggcaacgggg atttttccgc
    16321 ttgggggaat acgtagccgg gcgcactgga tcgatacctg gcaaatggct gatggcaagc
    16381 acgattacgc ctttgtgcat atgacgctaa aaatcggcag cggacgcagt ctggaaagcc
    16441 gtcaggaagt gggcgaaatg ctgtttgatc tgattaaaac gcacttcgcc tcactgatgg
    16501 agagccgtta cctagcgctt tcatttgaga ttgcagagct gcacccgacg ttgaatttca
    16561 aacagaacaa cgtacacgcg ttgtttaaat aaatttgcac cagcttccag gccggatgat
    16621 aaggcgctgc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
    16681 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
    16741 tcgctggatt acccggagat caccatcgaa gacgcctacg ccgtgcagcg cgaatgggtt
    16801 cgcctgaaaa tcgccgaaag tcgcacgctg aaaggccaca aaatcggtct gacctcgaaa
    16861 gcgatgcagg caagctcgca gatcagcgaa ccggattacg gtgcactgct ggacgacatg
    16921 ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
    16981 gagctggctt ttgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtt
    17041 tacaacgcca cggactatgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
    17101 atcgatccgg aaacccagcg cccgcgcaaa gtattcgaca ccatttccga taacgccgcc
    17161 aacgccggag tgatcctcgg aggtcgtccg atcaaaccag acgaactgga tctgcgttgg
    17221 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggtgtg
    17281 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
    17341 caactggaaa ccgggcaaat cattctcggt ggttcgttca ccctcccggt tccggcgcgt
    17401 aagggcgaca ccttccacgt cgattacggc aacatgggct ccattagctg ccgctttgtt
    17461 taaggagaga acgatggaaa acagttttaa agcggcgctg aaagcaggcc gtccgcagat
    17521 tggattatgg ctggggctga gcagcagcta cagcgcggag ttactggccg gaccaggatt
    17581 cgactggtta ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
    17641 actacaggcg attgcgccct accccagcca gccagtggta cgcccatcgt ggaacgatcc
    17701 ggtgcaaatc aaacaactgc tggacgtagg cacacaaacc ttactggtgc cgatggtaca
    17761 aaacgccgac gaagcccgta aagcggtacg cgccacccgt tatccccccg ccggtattcg
    17821 cggtgtgggc agtgcgctgg ctcgcgcctc gcgctggaat cgcattcctg attacctgca
    17881 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
    17941 cttaccgcag attctggacg tggaaggggt cgacggcgtg tttatcggcc cggcggatct
    18001 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
    18061 gcaggcgatc gtgcagatcc gcgaagcggg caaagcgccg gggatcctga tcgccaatga
    18121 gcaactggca aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac
    18181 caccctgctc gcccgcgccg ccgaagcgct ggcagcacgg tttggtaata gttcttctat
    18241 ttcgatggcg aagcaaaaca acaactctgt ctactaaacg tacgatgtgg agcgcatcat
    18301 gacgacctct accctgcaat ataaagataa taaaactgtt gaaattgaac atcgtgtcat
    18361 taataaatta tttcgtcgcc taatcatttt tctttttatt ctgtttgttt tttcgtttct
    18421 tgatcgcatc aatataggat ttgccggact gacaatgggc aaggatcttg gcctgacgtc
    18481 cacaatgttt ggcctcgccg caacactctt ttatgtcacg tacgtcatgt gcgggatccc
    18541 cagtaatatg atgttagcga aaattggcgc tcgtcgatgg atcgctggga tcatggtggt
    18601 ctggggaatt gcttccacct gcacaatgtt tgcaacaagc cccgaaacac tctatctact
    18661 gcgtatgctg gttggtattg ctgaggcggg ttttttacct ggtattttgg tttatctgac
    18721 ctggtggttc ccggcatatc atcgggccag agccaatgcc ttatttatga tagcgatgcc
    18781 agtcactatg atgctgggat tgatcctttc aggctacatt ctggccttgg atggactgtg
    18841 gaatctaaaa ggctggcaat ggctcttttt gctggaaggt ctgccatcgg tcattcttgg
    18901 tgtcgtcacc tggttttatc tcaacgacac accagaccag gcgacctggc ttgatgaaaa
    18961 cgaaaaacag gctttaaaaa cgatgattct ccgtgaacaa gagagtgcat ttgcccatgc
    19021 atctgttcca cgctcaacct tacgtgaagt cctgacccca gccatcatgc tatatacact
    19081 tgcttatttt tgcccgacta atactctgag tgcgattaat atctggacgc cgcaaatcct
    19141 acagagtttc aatactggca gtagcaacat catgattggt ttgctggcgg ccatccccca
    19201 attctgcaca atttttggca tgatctggtg gagtcgtcgt tccgaccgtc ttaaagaacg
    19261 caaaaagcat acgattcttc cctacctgtt tgccgctgcc ggatggttac tcgcttctgc
    19321 gacagatcac agcctcattc aactgagcag catcattatg gcctcaatgg gatcatttac
    19381 cgcgatgact atcttttgga caacaccgga tcaagttata agcttgcaat cgcgtgccgt
    19441 tgcgatggct gtcatcaatg ctgtcggcaa tgtaggttca gcagtcagtc cattacttat
    19501 cggtattttg aaagatgcaa ccggcagttt tagctcaggt ttgtggtttg tagcaggatt
    19561 gctggtactg ggcgcgttag tcatgacccg aatccctatg gggagtcggg aacactctct
    19621 ttcatctgaa cttatcgcac caaaaccgca ataaggagtc atgatgtcca gtgaagctat
    19681 ctttgaacat ggttatgaaa atattgattt ggtccgggaa tatgatgccc gctacgctgg
    19741 tgaagatgtg cattatgaaa catttgcccg gttagcgaat ttctttggac gcgatatgcg
    19801 ccctcactgg cacgatagat attttcaact ccattatctg gtcactggac gcattacttt
    19861 acaactggat gagcacttct attctgtgcg cgcaccgctt tttgtactaa caccaccatc
    19921 ggtgccacat actttcttta ctgatcctga tagcgatggg catgttctga ccgtacgtca
    19981 ggagttaatt tggcccctgg tagaaaagct ctggcctggt agtggagatg ccgcaatgct
    20041 acaaggtatc tgtctgtcac tggagggaat ggaacataca ctggcggcat taaatcatta
    20101 ttggccgata atatccctgg agtttcaaca aaataagcaa ggacgggaaa ttctattgca
    20161 atcactggcg caaagtattt ttacaatcct actgcgtgaa gcaccaccaa atgatatttc
    20221 aacatgcagc gtgcgcggcg agatgtgttt attccagaag tttaatcggc tgattgacga
    20281 aaattatcgc caacatttaa tggtccctga atatgcaaca atgctcggta tgagtgaatc
    20341 acggcttacc gagctgtgcc gacgttttgc caatcaatcg ccgaagagat tgattttcga
    20401 acgagtgagc cgggaagcca gacggcttct gctctatagc gagcaaagca tcaaccagat
    20461 agcccttgat cttggctaca aagatcccgc ctatttcgcc cgttttttca accgaatgat
    20521 gggttgctca ccaacacaat tccgtggtcg ctgaccattt atcaacctga gagactcgac
    20581 atgaaaatat ctttacgctc actatttgtg gcgtgcctga ttgcgccagt ggcattcacc
    20641 tacgcctccg aaccgcaata aatgaaaatt gtactacttg gaacgaaagg tggcccttct
    20701 ttattaaaag ctgaccgttt accgatggca acggcactta ttattggcaa taaaacctgg
    20761 ttaattgatg ctggttacgg ggccagctta caactggtaa aaaataatat tccactgcgc
    20821 cagattgata atattttact cacccattta cacagtgatc atattctgga ctatccatca
    20881 gtcttaatga atgcatgggc tagcggtctg caggacaaaa ctattcatgt attcggtccg
    20941 ccaggcaccc gagccatgac cgctgccagt tggcaggtct tcgatcggga catcacactg
    21001 cgtatggaag aagaaggaaa acccgatcct cgcaaactcg ttacagctca agatgttggt
    21061 gaaggcgtag tttataaaga tgataccgtg accgtgactg cattaaaggt ccctcattct
    21121 cctttccctg acggtgaggc ttttgcttat cgcttcgaca cacaaggcaa gcgaattgtc
    21181 ttctcaggag atacctcgta ctttccccca ctggcaactt tctcagaagg cgctgatata
    21241 cttgtgcatg aggccgtaca tgagccctcc gtcgctaagc tggcagacag tattggcaat
    21301 ggtaaaacac tggcaaaagc cattgcttct caccatacga ctattgagga tgtaggcaaa
    21361 attgccaatg aagcaaaagt gaaaaaactc atccttagcc acctggtacc aatcaccgtt
    21421 ccggatgaaa catggaagca agaagcacag aaaactattc aggtcctatc gtcgttggtc
    21481 atgaaaatat gacgattacc attccattcg ccccataaaa aattccgtcc ccgttagccc
    21541 ttgttagcgg ggaatattat gatttgcctt actgtaagtt gatcacgatc gcagtttact
    21601 atttaactcc accttgctgt cgaaaagtac caacgttcgc ctgaaacgtc tcttcccgac
    21661 aaccgcgttc aacgcttcaa ttaaataaca aaaacaacac acaaaattaa caatcgtatt
    21721 ccgattaata ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt
    21781 cctttcaccg gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat
    21841 ggcgagcgag tgaaagacgt caccactcat ccggcatttc gtaatgcggc agcgtctgtt
    21901 gcccaactgt acgacgcgct gcacaaaccg gagatgcagg actctctgtg ttggaacacc
    21961 gacaccggca gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccgacgac
    22021 ctgcgccagc aacgcgacgc catcgctgaa tggtcacgcc tgagctatgg ctggatgggc
    22081 cgtaccccag actacaaagc cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac
    22141 ggtcagttcg agcagaacgc ccgtaactgg tatacccgta ttcaggaaac tggcctctac
    22201 tttaaccacg cgattgttaa cccaccgatc gtcatttgcc gaccgataaa gtgaaagacg
    22261 tttacatcaa gctggaaaaa gagactgacg ccgggattat cgtcagcggt gcgaaagtgg
    22321 ttgccaccaa ctcggcgctg actcactaca acatgattgg cttcggctcg gcacaagtga
    22381 tgggcgaaaa cccggacttc gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga
    22441 aattaatctc ccgcgcctct tatgagatgg tcgcgggtgc taccggctcg ccatacgact
    22501 acccgctctc cagccgcttc gatgagaacg atgcgattct ggtgatggat aacgtactga
    22561 tcccatggga aaacgtgctg atctaccgcg attttgatcg ctgccgtcgc tggacgatgg
    22621 aaggcggttt cgcccgtatg tatccgctgc aagcctgtgt gcgcctggca gtgaaactcg
    22681 acttcattac ggcactgctg aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg
    22741 gtgtgcaggc cgatctcggt gaagtggtgg cgtggcgcaa caccttctgg gcattgagtg
    22801 actcgatgtg ttcagaagcg acgccgtggg tcaacggggc ttatttaccg gatcatgccg
    22861 cactgcaaac ctatcgcgta ctggcaccaa tggcctacgc gaagatcaaa aacattatag
    22921 aacgcaacgt taccagtggc ctgatctacc tcccttccag tgcccgtgac ctgaacaatc
    22981 cgcagatcga ccagtatctg gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc
    23041 agcgcatcaa gatcctcaaa ctgatgtggg acgccattgg cagcgaattt ggtggtcgtc
    23101 acgagctgta tgaaatcaac tactccggta gccaggatga gattcgcctg caatgtctgc
    23161 gccaggcaca aagctccggc aatatggaca agatgatggc gatggttgat cgctgcctgt
    23221 cggaatacga ccagaacggc tggactgtgc cgcacctgca caacaacgac gatatcaaca
    23281 tgctggataa gctgctgaaa taacgcagca ggaggttaag atgcaattag atgaacaacg
    23341 cctgcgcttt catgacgcga tggccagcct gtcggcagcg gtaaatatta tcaccaccga
    23401 gggcgacgcc ggacaatgcg ggattacggc aacggctgtc tgctcggtca cggatacacc
    23461 accgtcgctg atggtgtgca ttaacgccaa cagtgcgatg aacccggttt ttcagggcaa
    23521 cggcaagttg tgcgtcaacg tcctcaacca tgagcaggaa ctgatggcgc gccacttcgc
    23581 gggcatgaca ggcatggcga tggaagagcg ttttagcctc tcatgctggc aaaaaggtcc
    23641 gctggcgcag ccggtgctaa aaggttcgct ggccagtctt gaaggagaga tccgcgatgt
    23701 gcaggcgatt ggcacacatc tggtgtatct ggtggagatt aaaaacatca tcctcagtgc
    23761 agaaggtcac ggacttattt actttaaacg ccgtttccat ccggtgatgc tggaaatgga
    23821 agctgcgatt taagctccag ccccttcacg cattcgtcga aggggcattt ttattgtgat
    23881 ctcaatcaca tatctacccg atgaataatt cttgcccaac cgctcaccgc cattcctgac
    23941 cgcttacatc cctaaaataa ccactcagtt atttacctta ctttacgcgc acgtatctct
    24001 ggcaacatca ctgcaggata gcggtcaatt tacctcctca aacgcaacgc aaacctagaa
    24061 cggcttcggc ctatgattaa caaattactc tatggatttt ggtttgcagg aaggcagcaa
    24121 gtgagtgaat ccccgggagc ttacaaaagt aagtgtcaga ggtgaacgaa cgcagctgcc
    24181 gcacctgtaa gccaaaagac gacgagtaaa atgccaggtt ttactatgga tacgaaaaaa
    24241 ctattcaagc acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta
    24301 gttgcattac gtcgggggga gcacgtcagc gccctgtgga tcgtggtcgc ctctgtgtcg
    24361 gtgtatctgg tggcgtatcg ctactacagt ctgtacatcg cccagaaggt gatgaaactc
    24421 gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc
    24481 aaccgttacg tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtg
    24541 ggaccggttc tcgccgcgca gatgggctac ctgcctggca cgctgtggct gctggcgggg
    24601 gtagtgctgg ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac
    24661 ggcgcatctc tcggtgagat gatcaaagaa gagatggggc cagtaccggg gactatcgcg
    24721 ctgtttggct gtttcttaat catgatcatc atcctcgccg ttctggcgct gattgtggtt
    24781 aaagccctgg ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accgattgcg
    24841 ctgtttatgg gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtt
    24901 attggtatcg tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg
    24961 tactggggtc cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat
    25021 gcgtttgttt ccgcactgct gccggtgtgg ctgatcctcg caccgcgcga ctatctggca
    25081 accttcctga aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg
    25141 gaactgaaaa tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc
    25201 gctctgttcc cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgcg
    25261 ctgatctctt ccggtacgac gccgaaactg ctggctaacg aaaccgacgc gcgtttcatt
    25321 ggctacggcg caatgctgat ggagtccttc gtggcgatta tggcgctggt tgcagcgtcc
    25381 atcatcgaac cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc
    25441 atgcctaacc tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagctgaaa
    25501 gacgttaccg cacacgcggc agcgaccgtc agctcctggg gcttcgtgat ttcgccagag
    25561 cagatcctgc aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt
    25621 gcgccgacgc tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac
    25681 atgggcttct ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg
    25741 gatgcgggta cccgttctgg ccgctttatg ctgcaagacc tgctgggtaa cttcatcccg
    25801 ttcctgaaaa aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt
    25861 ctgtggggct acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg
    25921 tggccgctgt tcggtatctc caaccagatg ctggcagccg tagcgctggt actgggcacc
    25981 gttgtgctga ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta
    26041 tggctgctta tctgcaccac ctgggcgctg ggtctgaaac tgttcagcac caacccgcag
    26101 atggaaggct tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac
    26161 ctgacggcgc agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca
    26221 ggcctgagta ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc
    26281 tggcttgcgg tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc
    26341 ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg
    26401 ggctttgttc ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct
    26461 cggccaggcg gcgaagatgt tgattggtat tccagactac gacaactacg tcgagcatat
    26521 gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca
    26581 gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca
    26641 tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa actaccctgc
    26701 tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg
    26761 gcgaagtctc cgttgatgac caactgattg gcgaccgcgc cacgcagatc aaaacgctga
    26821 ccaacggctg catctgctgt tcgcgctcca acgagctgga ggacgcacta ctcgacctgc
    26881 tggataatct cgacaagggc aatattcagt tcgatcgtct ggtgattgaa tgcaccggca
    26941 tggccgatcc cggcccgatt attcagacct ttttctccca tgagatttta tgccagctct
    27001 acctgctgga cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc
    27061 agttcaccat cgcccagtcc caggtgggct acgccgaccg tattctgctg actaaaaccg
    27121 acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac gcccgcgcac
    27181 cggtctacac cgtcacccac ggcgacatcg acctgggcct gctgttcaac accaacggtt
    27241 ttatgctgga agagaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac
    27301 aaaacgatat ttcgtcaatt gtggtggaac tggattaccc ggtagatatc agcgaagttt
    27361 cccgcgtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt tacaaaggga
    27421 tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag cgcctctaca
    27481 gcgccgactg ggacaggccg tggggcgatg aaaaaccgca tagcacaatg gtgtttatcg
    27541 ggattcagtt gccggaggaa gagattcggg cggcgtttgc gggattgagg aagtaagttc
    27601 gggaaagatc aactttttga gagttccacc gcaacattat tacgagtgga actctttaga
    27661 tatgtcagac caaccaaagc ttcttcaatt gcgttttcat tttttcatgt ctcattttca
    27721 gttcagccaa tccccattct gaagaattac aaacatcttt tgttaaataa aaagagcttt
    27781 tactattaag tttctcataa atagatttct ttttgataaa atcatagtgt tgtgtttcag
    27841 aatttttgga accacttatg agtgttaaat tacctatttt atgcagccag tagacatgct
    27901 cttgaggttg gaatctatta atccagtatt cattaactaa tgcttgagga agaatatgct
//
LOCUS       DAJTRH010000051        27688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTRH010000051 DAJTRH010000000
VERSION     DAJTRH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(31..1026)
                     /gene="yjfF"
                     /locus_tag="OO339_002306"
                     /pseudo
     CDS             complement(31..1026)
                     /gene="yjfF"
                     /locus_tag="OO339_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1013..2035)
                     /locus_tag="OO339_002307"
     CDS             complement(1013..2035)
                     /locus_tag="OO339_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5926917.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2049..3551)
                     /locus_tag="OO339_002308"
     CDS             complement(2049..3551)
                     /locus_tag="OO339_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5926918.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3691..4647)
                     /gene="ytfQ"
                     /locus_tag="OO339_002309"
                     /pseudo
     CDS             complement(3691..4647)
                     /gene="ytfQ"
                     /locus_tag="OO339_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4957..5487
                     /gene="ppa"
                     /locus_tag="OO339_002310"
     CDS             4957..5487
                     /gene="ppa"
                     /locus_tag="OO339_002310"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5926919.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5867..6208)
                     /locus_tag="OO339_002311"
     CDS             complement(5867..6208)
                     /locus_tag="OO339_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5926920.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6211..9990)
                     /gene="tamB"
                     /locus_tag="OO339_002312"
     CDS             complement(6211..9990)
                     /gene="tamB"
                     /locus_tag="OO339_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5926921.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9987..11720)
                     /gene="tamA"
                     /locus_tag="OO339_002313"
     CDS             complement(9987..11720)
                     /gene="tamA"
                     /locus_tag="OO339_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5926922.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11926..12564
                     /gene="msrA"
                     /locus_tag="OO339_002314"
     CDS             11926..12564
                     /gene="msrA"
                     /locus_tag="OO339_002314"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5926923.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12887..14230
                     /locus_tag="OO339_002315"
     CDS             12887..14230
                     /locus_tag="OO339_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5926924.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14292..14498)
                     /locus_tag="OO339_002316"
     CDS             complement(14292..14498)
                     /locus_tag="OO339_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5926925.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14823..15377
                     /locus_tag="OO339_002317"
     CDS             14823..15377
                     /locus_tag="OO339_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5926926.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15440..16378)
                     /locus_tag="OO339_002318"
     CDS             complement(15440..16378)
                     /locus_tag="OO339_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926927.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16590..17330)
                     /gene="cysQ"
                     /locus_tag="OO339_002319"
     CDS             complement(16590..17330)
                     /gene="cysQ"
                     /locus_tag="OO339_002319"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5926928.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17520..19463
                     /gene="cpdB"
                     /locus_tag="OO339_002320"
     CDS             17520..19463
                     /gene="cpdB"
                     /locus_tag="OO339_002320"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5926929.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19589..19969)
                     /locus_tag="OO339_002321"
     CDS             complement(19589..19969)
                     /locus_tag="OO339_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5926930.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20058..20918
                     /gene="qorB"
                     /locus_tag="OO339_002322"
     CDS             20058..20918
                     /gene="qorB"
                     /locus_tag="OO339_002322"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5926931.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21027..21992
                     /locus_tag="OO339_002323"
     CDS             21027..21992
                     /locus_tag="OO339_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5926932.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22100..22762
                     /gene="ytfE"
                     /locus_tag="OO339_002324"
     CDS             22100..22762
                     /gene="ytfE"
                     /locus_tag="OO339_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5926933.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22807..24219)
                     /gene="cycA"
                     /locus_tag="OO339_002325"
                     /gene_synonym="dagA"
     CDS             complement(22807..24219)
                     /gene="cycA"
                     /locus_tag="OO339_002325"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5926934.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24527..25147)
                     /gene="fklB"
                     /locus_tag="OO339_002326"
     CDS             complement(24527..25147)
                     /gene="fklB"
                     /locus_tag="OO339_002326"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5926935.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25366..25911
                     /locus_tag="OO339_002327"
     CDS             25366..25911
                     /locus_tag="OO339_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5926936.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25895..26542)
                     /locus_tag="OO339_002328"
     CDS             complement(25895..26542)
                     /locus_tag="OO339_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5926937.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26754..27545
                     /locus_tag="OO339_002329"
     CDS             26754..27545
                     /locus_tag="OO339_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5926938.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27555..>27688
                     /locus_tag="OO339_002330"
     CDS             27555..>27688
                     /locus_tag="OO339_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5926939.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     "
BASE COUNT         6814 a         7377 c         6796 g         6701 t
ORIGIN      
        1 atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga ctcttgtcac
       61 tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg ctataaaaat
      121 aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc cgtcaaagtt
      181 gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa gcaccgtccc
      241 gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt ccagttccac
      301 acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa tccccgccag
      361 tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag tagaaatccc
      421 catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc caaaacgggt
      481 acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc ccatcgcact
      541 aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat aaatgggatg
      601 gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa agaacatccc
      661 cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc ccataaatgc
      721 gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg agaggccgaa
      781 atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac cgacggagag
      841 atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc caagaaaggc
      901 gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa actgggtcag
      961 gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca agttacgttt
     1021 tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga acaatcagca
     1081 cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag cccgaaagca
     1141 aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc agcaggttaa
     1201 agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg tccagctcca
     1261 gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg ataatgcccg
     1321 caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt cgcgtgttta
     1381 ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac atccccagcg
     1441 ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata atgaccggcg
     1501 ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag ttaaacgtga
     1561 cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc aggatcaggg
     1621 tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac aatcccgcca
     1681 ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc gcgaccgtca
     1741 tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga tcgataccac
     1801 cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct gcacggttaa
     1861 gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc cagaaatgcg
     1921 gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc ggcatccccg
     1981 tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga ggcatcacac
     2041 tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg tgccagcggg
     2101 atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata gcccaccagc
     2161 tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag cgtttcaatc
     2221 aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc atcaaggatc
     2281 agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg attgccaccg
     2341 gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg gcggataaag
     2401 cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc gcgctgggcc
     2461 tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc atcggttttc
     2521 ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga tcgcagggtt
     2581 tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc gaagatcact
     2641 tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat ctcgccgggg
     2701 cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt gaacgcggca
     2761 acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt atccagctcg
     2821 cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc ccgacagcct
     2881 acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac ctgatcgaga
     2941 aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat caggccaaat
     3001 aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat cagcactttg
     3061 gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac cgaaaaacgg
     3121 ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc ggtggcgcgc
     3181 ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc tatgaataga
     3241 ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt gccgataccg
     3301 agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag ccagatggtg
     3361 ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt tcccgccccg
     3421 ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac gttgtctaac
     3481 gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc ctggtgttgg
     3541 tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga taagacgcgg
     3601 taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg ccttatccgg
     3661 cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct tcttctctaa
     3721 ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca gcttttcagg
     3781 catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg ccatgttcgg
     3841 cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga tgtccggtac
     3901 accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt ctttaattgc
     3961 ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga tgtttttgcc
     4021 gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc gggtgaagtc
     4081 acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt cggcaaagcc
     4141 tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca ccacgttgca
     4201 tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt cgaggatgtt
     4261 gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg aacggtcgag
     4321 caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc aacccgtcgc
     4381 aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac gtaccgcttt
     4441 aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc cgcgtttttc
     4501 ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg atccgacctg
     4561 cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg ccgagactgc
     4621 agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat tattattggt
     4681 gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg cgttacatca
     4741 cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc atctcactat
     4801 gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa aactggcgaa
     4861 aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct ataatacttg
     4921 gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa cgtccctgcg
     4981 ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa cgcagatccg
     5041 atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt catgtccacc
     5101 gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct ggacggtgac
     5161 ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt gatccgttgc
     5221 cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa actggttgcg
     5281 gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa cgatctgcct
     5341 gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct cgaaaaaggc
     5401 aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga aatcgttgct
     5461 tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg aacgccgggc
     5521 ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc caccatcatt
     5581 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
     5641 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
     5701 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
     5761 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
     5821 tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac ttatccctgt
     5881 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
     5941 tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
     6001 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
     6061 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
     6121 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
     6181 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
     6241 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
     6301 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
     6361 tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt cgagcgctaa
     6421 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
     6481 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
     6541 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
     6601 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat cgtcttctgt
     6661 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
     6721 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
     6781 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
     6841 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
     6901 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
     6961 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
     7021 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
     7081 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
     7141 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
     7201 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
     7261 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
     7321 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
     7381 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
     7441 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
     7501 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
     7561 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
     7621 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
     7681 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
     7741 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
     7801 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
     7861 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
     7921 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc gggtattgct
     7981 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
     8041 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
     8101 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
     8161 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
     8221 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
     8281 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
     8341 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
     8401 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
     8461 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
     8521 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
     8581 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
     8641 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
     8701 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
     8761 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
     8821 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
     8881 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
     8941 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
     9001 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
     9061 acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg gccagttgtc
     9121 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
     9181 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
     9241 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
     9301 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
     9361 ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga ccacttcttc
     9421 ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
     9481 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
     9541 ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg tgatgggata
     9601 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
     9661 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
     9721 gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg cttttaccgc
     9781 cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
     9841 cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
     9901 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
     9961 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    10021 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    10081 ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg gcgaatatcg
    10141 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    10201 tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc gttggcgtat
    10261 ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc gacgaagaaa
    10321 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    10381 gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc ctggaaaacg
    10441 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    10501 tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    10561 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    10621 aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc caccagcgta
    10681 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    10741 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag ggtctgttcc
    10801 ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat ccacggcttt
    10861 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    10921 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    10981 ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga aagtcggcgg
    11041 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    11101 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    11161 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    11221 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    11281 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    11341 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    11401 ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    11461 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    11521 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    11581 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    11641 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    11701 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc cctggaacgc
    11761 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    11821 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    11881 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    11941 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    12001 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    12061 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    12121 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    12181 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    12241 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    12301 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    12361 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    12421 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    12481 gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg cggaattggc
    12541 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    12601 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgcccaaatt
    12661 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    12721 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    12781 actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    12841 atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt taaacagtat
    12901 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    12961 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    13021 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    13081 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    13141 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    13201 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    13261 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    13321 cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    13381 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    13441 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    13501 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    13561 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    13621 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    13681 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    13741 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    13801 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    13861 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    13921 tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg acgacgtcat
    13981 gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca tcggcggctt
    14041 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    14101 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    14161 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    14221 cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct tattactgca
    14281 tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt aacttcggac
    14341 atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc cttatcaaat
    14401 tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt tacgtacttc
    14461 gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg ctgagtatta
    14521 cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta atgcttagtg
    14581 actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct aaaggttaca
    14641 attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt catttttcat
    14701 atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg ataaatagca
    14761 catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa gaaggaaaca
    14821 ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg atggcttccg
    14881 cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat cggggcgagt
    14941 tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag ttagtgggaa
    15001 aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa aacgcgacgc
    15061 tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc accaccattg
    15121 ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt ctggagagta
    15181 ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc gcgcgcggtg
    15241 cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac ggtcgcgtgc
    15301 aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg gacctgctgc
    15361 aaaagttgct caaataatgg caataacata aattctggaa aaataatttt cccctatcgt
    15421 tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat actggtaacg
    15481 ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa atcttttctt
    15541 tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact tgttcggcta
    15601 attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag taatcttcat
    15661 cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag attttaagcg
    15721 caaagcccaa agattctatg actatggcaa aattatcatc aattattttt tcgtaaatat
    15781 accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat ttaccccgtt
    15841 ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca ccaaacgcct
    15901 ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc aaaatatcac
    15961 aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa tccatgaatg
    16021 agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg cctcgcaatg
    16081 ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa ttatccatgg
    16141 cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca atctgtgttg
    16201 cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc caatcttttt
    16261 tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt gtatgcgatg
    16321 actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg ggtaacatat
    16381 ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt atagaagtaa
    16441 actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt tttgcacttt
    16501 aaatacaatc tctttttatt tataactttt taggttggga taaatcagga tggcagaatc
    16561 aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa ccccggattc
    16621 aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg aacgtgcgct
    16681 ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt tggtccgaag
    16741 cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga agagccgatg
    16801 gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc atctgcatgg
    16861 gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag cttgcgcaca
    16921 ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt cattaccggc
    16981 gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt aacggtgaat
    17041 tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag ccagtaacgc
    17101 tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac cggaatatcc
    17161 ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc cgctgccgtt
    17221 accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc gtagacctgc
    17281 ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat ttctccacct
    17341 cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta atgaatgcgc
    17401 caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc actgttctat
    17461 aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg atgtccctga
    17521 tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg aatgcagcga
    17581 cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg gatttcgatt
    17641 attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc ctgattaacg
    17701 atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg attcagggga
    17761 gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt catccggttt
    17821 ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat gaatttaact
    17881 acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat gtaaatgcca
    17941 acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt aaagataccg
    18001 aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt ggcgtcgtgc
    18061 cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg gtgaatgata
    18121 ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc gatgtcattg
    18181 tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg gaaaactcag
    18241 tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat gctcacgccg
    18301 ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa ggcacgctga
    18361 atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg gtcgacttac
    18421 aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa gcacggccga
    18481 tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg gtagaaacac
    18541 tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt aaatctgccg
    18601 acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa gtcgtgaaca
    18661 acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg gcaaaactgc
    18721 cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac ccggcaagct
    18781 atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt tatctctacc
    18841 ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg ctggagtgct
    18901 ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca ctcatcaact
    18961 gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat cagattgatg
    19021 ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat gcagagcgga
    19081 ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc ctggttgcca
    19141 ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc catatcgctt
    19201 ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat gagtcgaaac
    19261 gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg atagctgccg
    19321 ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca gcgtttatta
    19381 aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg tttgcgattt
    19441 atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca accgcatccg
    19501 gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc atgaaccgta
    19561 gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc accgccaaca
    19621 cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg ctcacctgct
    19681 cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac gcgatacggt
    19741 taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt tcactcaccc
    19801 caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc gccaccagaa
    19861 tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg cactgttccg
    19921 caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt tcatacttac
    19981 ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa cacaagacaa
    20041 aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt ggtcactata
    20101 ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc gttcgtaatc
    20161 cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct gactacagcg
    20221 atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg atctcttcca
    20281 gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca aaggcggctg
    20341 gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg ctcggcctcg
    20401 ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt tacaccctgc
    20461 tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca ctggaacacg
    20521 gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg gcagattatg
    20581 cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt tacgaactgg
    20641 cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa cagagcggca
    20701 aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg aaaagcgtcg
    20761 gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca tcgaaaggtg
    20821 gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg acaacgttag
    20881 ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg gcatcctccc
    20941 gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc tgaacgtttc
    21001 ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc ctgttagcag
    21061 ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac ccggcgatgt
    21121 tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt gcctggctgg
    21181 gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg atgctcacta
    21241 tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt actggcgcgc
    21301 ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt gccaatctgc
    21361 tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca ctgatttgta
    21421 ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc cccgattttg
    21481 actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc tgctgggcat
    21541 ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat ccgatgatgt
    21601 gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat ctcgtcgcct
    21661 gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc cgtccgctgg
    21721 tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc gcactctgct
    21781 ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt gttttcgaaa
    21841 cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg ctaatgacgc
    21901 tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca aaaccagaaa
    21961 aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta aataagcctc
    22021 tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat cttatcttat
    22081 taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac tggcgctctc
    22141 tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct gtggcggtaa
    22201 gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca ttgaagctga
    22261 actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc cgctggcaga
    22321 aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc tgccggagct
    22381 gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg tgccaaaagg
    22441 gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga tgaaagaaga
    22501 gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg ggccaatcag
    22561 cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca aacacactac
    22621 caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt ataacggcat
    22681 taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg tactgttccc
    22741 acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc cagcatgata
    22801 atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc agcccaaccc
    22861 cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat cttccagtgt
    22921 cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca gcttgccgag
    22981 cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt acaccaggta
    23041 agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg ttgtaatcat
    23101 cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc cgagcagaca
    23161 gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt tagcgaacgc
    23221 tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag agaagacacc
    23281 gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc tggcagccgc
    23341 aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga ctaccgaact
    23401 ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa tgatacggat
    23461 cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt ccgcagctgt
    23521 tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc cggcaaagaa
    23581 gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga atgacgcttc
    23641 cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga caatcagcga
    23701 cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga acattttcac
    23761 ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga cccagtcgga
    23821 gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg ccataccggt
    23881 tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg gccccagtaa
    23941 atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca attcccccat
    24001 tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa tgatcgacgg
    24061 cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa tggcacctcc
    24121 aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact gttcagccgg
    24181 agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc ctgtacctgt
    24241 ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat ctgtatcggg
    24301 actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat ctaatgttaa
    24361 ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc acaaaaatac
    24421 acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa gggtttattg
    24481 ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag aggatttcca
    24541 gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct gcgccgcgct
    24601 cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc ggcatcagag
    24661 tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg ggttcaccac
    24721 gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa tgaacacgaa
    24781 cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg aattgcaggc
    24841 cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat ttcacgcctt
    24901 cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg tggatttcac
    24961 gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc agcgcatcgg
    25021 caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca ctcagttgtt
    25081 gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca aaagttgggg
    25141 tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc agccacactt
    25201 ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag aaacaatagc
    25261 aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc gttagcttat
    25321 tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc cgggcgcttt
    25381 gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg cttcatggac
    25441 ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt ggtggtcgga
    25501 tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga agcgcagtta
    25561 gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac ccaaacgcaa
    25621 ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt
    25681 aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg
    25741 gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca gatggtgaaa
    25801 atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa
    25861 caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt
    25921 caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct
    25981 ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg atcaaataat
    26041 atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct ggcttaaaaa
    26101 tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga gcatggttaa
    26161 tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt tcactatgca
    26221 caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca ataattttct
    26281 caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt tgtcgaatat
    26341 cgttccaggt atattccagc acttcgataa ataaattttc tttattacca aagtggtgat
    26401 aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg gccccttcat
    26461 aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc aggctctgta
    26521 cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg actatcataa
    26581 cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat caggttatca
    26641 aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt ttatcctcca
    26701 gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat tgtatgaaat
    26761 ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag ggctgtgcgc
    26821 aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat gccgacagcg
    26881 tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa gtgatttgct
    26941 tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg gcgcaaaacc
    27001 agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa aaacgcggtg
    27061 acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac acccgcgcgc
    27121 cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa ccggaagcgg
    27181 agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg ctggcgatga
    27241 atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt ctgtttgctg
    27301 ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc atcaaaactg
    27361 atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt ctggtgccgt
    27421 ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg gcggaaggta
    27481 aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt atgccgcgct
    27541 tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt atcaataccg
    27601 cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc gcacgcggta
    27661 ttggcgcaat tgccccctgg cgcaggga
//
LOCUS       DAJTRH010000052        26898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTRH010000052 DAJTRH010000000
VERSION     DAJTRH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO339_002331"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO339_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5926940.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO339_002332"
     CDS             complement(1614..1937)
                     /locus_tag="OO339_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5926941.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO339_002333"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO339_002333"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5926942.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO339_002334"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO339_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5926943.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO339_002335"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO339_002335"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5926944.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO339_002336"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO339_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5926945.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO339_002337"
     CDS             7537..8574
                     /locus_tag="OO339_002337"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5926946.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO339_002338"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO339_002338"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5926947.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO339_002339"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO339_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5926948.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO339_002340"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO339_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5926949.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO339_002341"
     CDS             10745..11626
                     /locus_tag="OO339_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5926950.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO339_002342"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO339_002342"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5926951.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO339_002343"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO339_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5926952.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO339_002344"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO339_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO339_002345"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO339_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5926953.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO339_002346"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO339_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5926954.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO339_002347"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO339_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5926955.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO339_002348"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO339_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5926956.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO339_002349"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO339_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5926957.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO339_002350"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO339_002350"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5926958.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO339_002351"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO339_002351"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5926959.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO339_002352"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO339_002352"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5926960.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO339_002353"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO339_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5926961.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO339_002354"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO339_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5926962.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO339_002355"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO339_002355"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5926963.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO339_002356"
     CDS             24893..25273
                     /locus_tag="OO339_002356"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5926964.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO339_002357"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO339_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5926965.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO339_002358"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO339_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5926966.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..26898
                     /locus_tag="OO339_002359"
                     /pseudo
     CDS             26442..26898
                     /locus_tag="OO339_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6639 a         6691 c         7055 g         6513 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccg
//
LOCUS       DAJTRH010000053        26371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTRH010000053 DAJTRH010000000
VERSION     DAJTRH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            176..469
                     /locus_tag="OO339_002360"
     CDS             176..469
                     /locus_tag="OO339_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5926967.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            541..1200
                     /locus_tag="OO339_002361"
     CDS             541..1200
                     /locus_tag="OO339_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5926968.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1292..1738
                     /locus_tag="OO339_002362"
     CDS             1292..1738
                     /locus_tag="OO339_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5926969.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1945..2845)
                     /gene="hlyE"
                     /locus_tag="OO339_002363"
                     /pseudo
     CDS             complement(1945..2845)
                     /gene="hlyE"
                     /locus_tag="OO339_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3218..3637
                     /locus_tag="OO339_002364"
     CDS             3218..3637
                     /locus_tag="OO339_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5926970.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3637..4905
                     /gene="umuC"
                     /locus_tag="OO339_002365"
     CDS             3637..4905
                     /gene="umuC"
                     /locus_tag="OO339_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5926971.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(4951..5481)
                     /gene="dsbB"
                     /locus_tag="OO339_002366"
     CDS             complement(4951..5481)
                     /gene="dsbB"
                     /locus_tag="OO339_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5926972.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5627..7168)
                     /gene="nhaB"
                     /locus_tag="OO339_002367"
     CDS             complement(5627..7168)
                     /gene="nhaB"
                     /locus_tag="OO339_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5926973.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7389..8108
                     /gene="fadR"
                     /locus_tag="OO339_002368"
     CDS             7389..8108
                     /gene="fadR"
                     /locus_tag="OO339_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5926974.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8160..9692)
                     /locus_tag="OO339_002369"
     CDS             complement(8160..9692)
                     /locus_tag="OO339_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5926975.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10022..11320
                     /gene="dadA"
                     /locus_tag="OO339_002370"
     CDS             10022..11320
                     /gene="dadA"
                     /locus_tag="OO339_002370"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5926976.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11330..12400
                     /gene="dadX"
                     /locus_tag="OO339_002371"
     CDS             11330..12400
                     /gene="dadX"
                     /locus_tag="OO339_002371"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5926977.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12568..13982)
                     /locus_tag="OO339_002372"
                     /pseudo
     CDS             complement(12568..13982)
                     /locus_tag="OO339_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14593..16329)
                     /locus_tag="OO339_002373"
     CDS             complement(14593..16329)
                     /locus_tag="OO339_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5926978.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16424..17338)
                     /gene="ldcA"
                     /locus_tag="OO339_002374"
     CDS             complement(16424..17338)
                     /gene="ldcA"
                     /locus_tag="OO339_002374"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5926979.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17438..18049
                     /gene="emtA"
                     /locus_tag="OO339_002375"
     CDS             17438..18049
                     /gene="emtA"
                     /locus_tag="OO339_002375"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5926980.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18051..18785)
                     /locus_tag="OO339_002376"
     CDS             complement(18051..18785)
                     /locus_tag="OO339_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5926981.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            18986..19240
                     /locus_tag="OO339_002377"
     CDS             18986..19240
                     /locus_tag="OO339_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5926982.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19290..20750)
                     /locus_tag="OO339_002378"
     CDS             complement(19290..20750)
                     /locus_tag="OO339_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5926983.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20687..21244)
                     /locus_tag="OO339_002379"
     CDS             complement(20687..21244)
                     /locus_tag="OO339_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5926984.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21270..22124)
                     /locus_tag="OO339_002380"
     CDS             complement(21270..22124)
                     /locus_tag="OO339_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5926985.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22124..22933)
                     /locus_tag="OO339_002381"
     CDS             complement(22124..22933)
                     /locus_tag="OO339_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5926986.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(22943..23701)
                     /locus_tag="OO339_002382"
     CDS             complement(22943..23701)
                     /locus_tag="OO339_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5926987.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23698..24678)
                     /locus_tag="OO339_002383"
     CDS             complement(23698..24678)
                     /locus_tag="OO339_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5926988.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24678..25700)
                     /locus_tag="OO339_002384"
     CDS             complement(24678..25700)
                     /locus_tag="OO339_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5926989.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26050..>26371
                     /locus_tag="OO339_002385"
     CDS             26050..>26371
                     /locus_tag="OO339_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5926990.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTG"
BASE COUNT         6619 a         6430 c         6816 g         6506 t
ORIGIN      
        1 tccagtggct tttctgcgtt cggtaaaatc ccggcgcggc attttttgcc ggggggatta
       61 cttataaagg ggcatggact caacaaaccg cagaaatcta cagctggctg gcttgtggcg
      121 cgggtttcat tgatgttagc ttatgccaaa acctggcatc ttaaagagta aaagtatgtt
      181 ttgtgcgatt tatcgaagca gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga
      241 cgatttttcg cgtgttcctg aggaactgat gaaaggtttt ggtcagcctc agttagcgat
      301 gattctgccg ttggatgggc ggaagaaact ggtcaatgct gatattgaaa aggttaaact
      361 ggcattaacc gagcaaggtt actatttgca gttaccgcca ccacccgaag atttgctgaa
      421 gcaacatctt tccgtcatgg ggcagaaaac agacgacact aacaaataac cgatatccgg
      481 cggtggcatt atctttgtcg gcgcgggttt tcatatccac gataaggtga ggggaacgtt
      541 atgtatcaac atcacaactg gcaaggtgcg ctgctggatt atccggtgag taaagtagtc
      601 tgtgttggca gtaactatgc caaacatatt aaagagatgg gcagcgcagt gcccgaagag
      661 ccagtgctgt ttattaaacc agaaacggca ctgtgcgatc tgcggcagcc attggcgatc
      721 ccatccgatt tcggttcagt tcatcatgaa gtcgaactgg cggtgttgat tggcgcgacg
      781 ctgcgtcagg caacggaaga gcatgtccgc aaagccattg ccggttacgg cgtggcgctt
      841 gatctgacat tgcgtgatgt tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag
      901 gctaaagcgt ttgataactc ttgtccgctt tccgggttta tccccgcggc ggaatttacc
      961 ggcgatccac aaaatacaac gctgggcctg agcgtaaacg gcgaaaaacg ccagcaaggt
     1021 acgactgcgg acatgatcca taaaatcgtt ccgttgatcg cttatatgag caagtttttt
     1081 accctcaagg ccggtgacgt tgtgttgaca ggcacgcctg atggcgtcgg cccgttgcaa
     1141 agcggtgatg agctgacagt cactttcgat gggcattctt tgacaactcg cgttttgtaa
     1201 tactttttgc cgccagaaag cggcggcaaa acttgcatcg ctgtgccaga ctggttataa
     1261 ggtgcgtttt aacgtaatgg cggaacacct gatgagcgat gtacctttct ggcaaagtaa
     1321 aaccctggac gaaatgagcg atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg
     1381 ttgcttgcat aaactgatgg atgaagacac cgacgaaatc tacttcacta acgtcgcctg
     1441 tcgccagctc aatattaaaa cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga
     1501 acccgactgc attaaattaa cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac
     1561 ctgcgcttat cggttgctgg cggaaggtaa agatttacct gcgtggcatc cgctacttac
     1621 tggttcgaaa gcggcaatgc atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga
     1681 atcagaagtg attgactggc aggatcatat cttaaataaa cctgactggg cgcagtgata
     1741 aatttaatag atgctgatgg gggctttctt aatatacgtc tggatgctcg ccatttcgta
     1801 gccctgtttt tgaaagcgct ctgtcataat gagagttacc cgatacctta ttatcaacag
     1861 gtaactctca atctttaaaa aacgtctgtg tttttaaatg tgataaacct tataccttga
     1921 gtacatggag agaataatcg cttatcagac ttcaggtact tcaaagagtg tctttttacc
     1981 gtgtcttttc tgatactcat tacaggtgtt aatcattttt ttggccgctg cttttagcaa
     2041 agaaagcatt aaatcatcat aatcaacata gaatctggtt gtttcagttt ccgtttttat
     2101 ctccccgatg gcggctattt cggtggttaa tttcaatttg gcggcatcga tatctttatt
     2161 cgcttgttta accgtgttag acagggtggt aaagaaactc tgcacagatt ttaacttgtt
     2221 cttcaattct ggaatcagtt tcccttcaac tacgcccgca gcaatagaat aggaaatgat
     2281 taaaccaaat ggaccggcga cgacatcggc tgcggcaccg gcatacgctt ccttcctgat
     2341 tttatctacc tgtgactgga aatagctgct tttttctgaa aaatcattgg ttaactggct
     2401 atctaacgcc agcagtttcc cggaagcgtt gttgaaactt tgtgagctta ccagcaggga
     2461 tttttgcgct tcattcagct tcgtgatgcc gtcatccagt accttaatga ggggcggatg
     2521 ctttcttctc attgtactca tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa
     2581 caccacacca ttcatacact gtttgggttg cttcaaaata cttatcctgg ctatccataa
     2641 gtaaggtttt aatatcgccg actaaaacgg aggctgcctg tgaatactcc tgtttaaagc
     2701 gacttaactc ttttatggtt tcatcaaagg tctgccaggg gatgacctga tcgagatatt
     2761 tattataaag atctaatgct ccatctgcgg tttcgattgc gtttttaact acttctaccg
     2821 ttttatctgc aacgatttca gtcataatca ttcgcctctt taaatatata aattgtaatg
     2881 aaactcctgt tttacaacta ttaataaatt ttacttcatc taattcatag ttagccgggc
     2941 gggatgcgtc aatgtcttta tttctattaa tatgataaat atcaaacaat gtttaatgtc
     3001 attatggcga atgcttctat tctatttttt tgccgggtga tatttttcat ttctgctgga
     3061 tgagcgtcgt cgccagaagg ccacgtgagc acaggataag agaacgaaaa atcagcagtc
     3121 tatgcagcga caaatattga tagcctgaat cagtattgat ctgctggcaa gaacagacca
     3181 ctgtatataa aaacagtata acttcaggca gattattatg ttgtttatca agcctgcgga
     3241 tctccgcgaa attgtgactt ttccgctatt tagcgatctt gttcagtgtg gctttccttc
     3301 accggcagca gattacgttg aacagcgcat cgatctgaat caactgttga tccagcatcc
     3361 cagcgcgact tacttcgtca aagcaagtgg tgattctatg attgatggtg gaattagtga
     3421 cggtgattta ctgattgtcg atagtgctat taccgccagc catggtgata ttgtcatcgc
     3481 tgctgttgac ggcgagttta cggtgaaaaa attgcaacta cgcccgacgg tacagcttat
     3541 tcccatgaac agtgcgtact cgcccattac catcagtagc gaagatacgc tggatgtctt
     3601 tggtgtggtg atccacgtcg ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac
     3661 gcgttttatg ccagctgtga gacggtgttt cgccctgatt tatggggtaa accggtggtt
     3721 gtgctatcga ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggc
     3781 gttaaaatgg gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt
     3841 tgctttagca gcaattatga gctttacgca gacatgagca atcgggtgat gtcgacgctg
     3901 gaagaactat cgccccgcgt cgagatttac agtattgatg aagcattctg cgatctgaca
     3961 ggtgtgcgta attgtcgcga tctgactgat tttggcagag aaattcgcgc aacggtgcta
     4021 caacgtaccc atcttactgt tggtgtgggg atcgcccaga ccaaaacgtt ggctaagctt
     4081 gccaatcatg cggcaaaaaa atggcagcgg cagacgggtg gggtggtgga tttatcaaat
     4141 ctggaacgcc agcgtaaatt aatgtctgct ctccccgtgg atgacgtctg ggggattgga
     4201 cggcggatca gcaaaaaact ggacgcgatg gggatcaaaa ccgttctcga tttggcggat
     4261 acagatatcc ggtttatccg taaacatttt aatgtcatgc tcgaaagaac ggtgcgtgaa
     4321 ctgcgcggcg aaccctgttt gcaactggaa gagtttgcac cgacgaagca ggaaattatc
     4381 tgttcccgct cgtttggtga acgcatcacg gattatacgt cgatgcggca ggccatttgt
     4441 agttacgctg cccgggcggc ggaaaaactt cgcagtgagc atcaatattg tcggtttatc
     4501 tccacattta ttaagacgtc accatttgcg ctcaatgaac cttattacgg caatagcgcg
     4561 tcggtaaaac tgctgacgcc cactcaggac agcagggata tcattaacgc tgctacgcga
     4621 tctctggatg ccatctggca agcgggccat cgctaccaaa aagcgggcgt gatgctgggg
     4681 gatttcttca gtcagggagt cgcgcagctc aatttattcg atgacaacgc accgcgcccc
     4741 gggagtgagc aattgatggc ggtaatggat actctgaatg ctaaagaggg cagaggaaca
     4801 ctctattttg ccgggcaggg gatccaacaa caatggcaga tgaagcgagc tatgctttca
     4861 ccacgttata caacgcgaag ttctgattta ctgcgggtca aataaatata gcggcaggaa
     4921 aaaagcgctc ccgcaggagc gccgaatgga ttagcgaccg aacagatcac gttttttcgc
     4981 tttaaacggc tgggaaatca ccaccagcac cgcgacaatc aggtaagcga taaaaatacc
     5041 gagcagccac tgcggcattt ccatacctaa aaaatcccac tgacgctcgg cgcaatcgcc
     5101 agaggcgaca aacacttgcg gcacccattt atccagcggc agccattccg ggaaacgaac
     5161 cataaaatca caggtggcaa atggcgaagg atagagctga agcatggtgt gctcgtaagt
     5221 taactgcaca ccgcggaacg cactatacaa ccagataacc atcgctacat aacgcagcgg
     5281 agttttcggg gcgatcgcgc caatcagcgc agcacccaga acgccgaata acgcgcagcg
     5341 ttcataaata cagagcacgc aaggtttcag taacatcaca tgctggaacc acagcgccgt
     5401 cagttccagt gccagagcag taaacgccat caacagccac gcgccccggc cttgtgaaca
     5461 ttggttcaaa aatcgcaaca taatcatttc cctgcaatat gcatagagtg cgcagtttaa
     5521 accaattcat tcgctgcgcc accaggaggc agcaaaaata ggacgcaatt ggacaattat
     5581 cgggcgaaaa ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa agcgttgcta
     5641 tccagcccat ttgcataaac cattcggtta caggggcaag cgtaaactcg acgcagagca
     5701 agccgacgag tgtcagtacg agggtgtaag gcagggccat ccacaccatg cggccataag
     5761 agaggcgaat caatggcgcg agtgcagagg tcagcaggaa caggaacgca gcctgaccgt
     5821 tcggcgtagc gacagagggc agattggtac cggtattaat ggcgaccgcc agcagctcgt
     5881 attgcttcaa cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg atataaatcg
     5941 tccccacgaa gacgttatcc gaaatggatg acagcagacc gttgaaaata tagaacagcg
     6001 acagctgagc atgttcagat gcctgcaaca caaactgaat aatcggcgaa aacagttgtt
     6061 ggtcgataat caccgcgact accgagaaaa acaccgtcaa cagtgcggtg aatggcagag
     6121 attcggtgaa ggctttaccg atagcatgtt catcggtgac accggtcaat gatgttgcca
     6181 gaataatgac tgacaaacca atcaagccaa cttccgccag atgcaacgcc agcgcagtca
     6241 ccagccagac gccaataatc gcctggacaa tcagacggat tttatcctga cgggtacgct
     6301 ggtggcggct ttgatcgtca aactgttgca acacttcgcg gactttctcc ggcagcgttt
     6361 caccgtagcc aaaccaacgc agcttctcta ccagcaggca ggttaacagg ccacaaatca
     6421 gaaccggaac ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc cagccagccg
     6481 ctttagcgat gatcaggttc tgtggttcgc ccaccatggt cattacgccg cctaatgcgg
     6541 tgccgacacc ggcatgcatc atcaggctac gcagaaagcc acggaactgt tccagaacca
     6601 cgttgtaatg cttgtcgata tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag
     6661 aggctacgcg atgataaata ccataaaaac cgactgcaac gctgatcacc accgccacga
     6721 cggttaaggc atcgaggaac gcggagagga acgctgccgc cacgcaaaaa gagagcgaca
     6781 gcagcatttt ggagcgaatg cttaacagca aacgggtaaa tatgaacagc aacagctgtt
     6841 tcataaaata gatacccgcc accataaaca tcaataacag caagacttca agatttgccg
     6901 ccacctcttc acggacgtgt tccgcgctgg tcatgccgat gaataccgct tcgatagcca
     6961 acagaccacc ggggagtagc gggtagcatt tcagggccat cgccagagtg aaaataaatt
     7021 ccgctaccag caaccagccc gcgacgaaag ggctgatgag gaaaattaac gggtttacga
     7081 ttaagaaaat gatgagggcg agtttgtacc agtcggggga ctggcccaaa aaattgcgcc
     7141 atagcgcgcg gccccaggag atctccatga tggtttccct taccttacaa ataatcaatg
     7201 atgtttttat gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca agtcttgata
     7261 gtcaagggga aagagatgcg gaaaatgaag ccttgatccc tttttcttct ttttgtctgc
     7321 tatcagcgta gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa
     7381 atctcactat ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg
     7441 aaagtatctg gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt
     7501 cagaattaat tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt ctggcacgag
     7561 atggctggtt gaccattcaa catggcaagc cgacgaaggt gaataatttc tgggaaactt
     7621 ccggtttaaa tatccttgaa acactggcgc gactggatca cgaaagtgtg ccgcagctta
     7681 ttgataattt gctgtcggtg cgtaccaata tttccactat ttttattcgc accgcgtttc
     7741 gtcagcatcc cgataaagcg caggaagtgc tggctaccgc taatgaagtg gccgatcacg
     7801 ccgatgcctt tgccgagctg gattacaaca tattccgcgg cctggcgttt gcttccggca
     7861 acccgattta cggtctgatc cttaacggga tgaaagggct gtatacgcgc attggtcgtc
     7921 actatttcgc caatccggaa gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg
     7981 cgttgtgcag tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg
     8041 agagtggcga gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg
     8101 ggcgataatc ccttccgttt aaagagcaac cccctcaaac gaggggtttt ttgttgtttt
     8161 tacagatttc ccattcttgg cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt
     8221 tgctgttcga gcatcacatc aaatccccac aggcgatgca catgcttcag gacttctttg
     8281 cgcccccgat ccagcggtgc gcgattatgt ggaatataac gcagcgtcag cgaacggtcg
     8341 ccgcgcaaat ccacgttcca gatctgaata ttcggctcca gattacttaa gttatattgc
     8401 gacgataacc ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc
     8461 agataattat gccgatcatc atccagcacg gtgaagaagc ggaaatcacg catcactttc
     8521 ggtgacagaa actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc
     8581 gtttccagcc agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc
     8641 gactgacaaa tccgtttaat atcctggaac atggcgaacc cgagggcata cgggttgatg
     8701 ccgctgtacc acgggctgtt ataggggggc tggaagacca cattggtgtg gctgtgcaaa
     8761 aactccagca taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggta
     8821 tagtgccaga aggtcgcaca gccttcgttc atcacctgag tctgtttttg cggataaaaa
     8881 tactggctca ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc
     8941 gcatttttct ccataaaata gagcaggttt tcttgtggtt cggacggata gcggcgcgct
     9001 tcagcaaccg ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga
     9061 ctttgtagat actcttcgcg acttttctgc cgggctttct cttcttgcag cgagattttt
     9121 tgcgggcgtt tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc
     9181 cgttctactt catcaacgcc ataacgttct tcgcactcgg taatatattt acgggcaaaa
     9241 atcagataat cgacaatcga actggcgtcg gtccaactgc ggaataagca attgttttta
     9301 aagaaagagt tatgtccata gcaggcgtgt gccatcacca gcgcctgcat agtaatggtg
     9361 ttctcttcca tcaagtaagc gatacacggg ttagagttaa tgacgatttc ataggccagc
     9421 ccttgctgac cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag
     9481 tgcggatagt taattggcat gccgacgctg gaataggcat ccatcatctg ttctgaggtt
     9541 atcacttcaa tctggtgcgg gtaggtatcc agccggtaga gtttcgccac ccggtctatc
     9601 tctgccagat aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg
     9661 gtgtccttat tcatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct
     9721 ctgtgtggag cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa
     9781 tttttttctt tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt
     9841 tatgctggat aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt
     9901 cagggagatg tgagtcagct caccataaaa aagccgtatg ttgaataata ttttcaactg
     9961 agttatcaag atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc
    10021 tatgcgagtt gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa
    10081 tcaggcagga catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag
    10141 tgccgcgaat gccgggcaaa tctcccccgg atatgctgca ccgtgggcag caccaggtgt
    10201 gcctttaaaa gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga
    10261 cggtacgcag tttcagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca
    10321 ctacatggaa aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa
    10381 agcattgcgc gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact
    10441 gttccgtacc gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc
    10501 cggcgtaccg tatcagctgc tggaatccag ccgcctggcg gaagtggatc ccgcgctggc
    10561 agaagtagcg cacaaactga cgggcggcct gcagttaccc aatgatgaaa ccggagactg
    10621 ccagctgttt acccagaatc tggcgcggat ggcagagcag gcgggggtta aattccgctt
    10681 taatacgccc gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtttgg
    10741 cgatgaagtg attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat
    10801 gctcaaaggc attgttgata ttccggtgta tccgctgaaa ggctactcgc tgactattcc
    10861 aattgcgcag gaagatggtg cgccagtatc caccattctt gatgaaacct acaaaatcgc
    10921 cattacccgt ttcgataacc gcattcgtgt tggcggaatg gcggaaattg ttggttttaa
    10981 caccgagctg ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc
    11041 acgcggcggt catgtcgagc aggcgacttt ctggacgggt ttgcgcccga tgacgccaga
    11101 cggtacgccg gttgtcggga gtacacgctt taaaaatctg tggctgaata ccggtcacgg
    11161 cacgctcggc tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg
    11221 tcgcacgcca gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac
    11281 gccatcacgt ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc
    11341 gatacaggcc agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc
    11401 cgcgccgcac gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc atggtattga
    11461 gcgtatctgg agcgcgctcg gggccaccga tggctttgca ttacttaacc tggaagaggc
    11521 aataacgtta cgtgagcgcg gctggaaggg gccgatcctg atgctggaag gatttttcca
    11581 tgctcaggat ctggagattt atgaccagca ccgcctgacc acctgcgtac acagcaactg
    11641 gcagctcaaa gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt
    11701 gaacagtggg atgaatcggt tgggcttcca gtccgatcgc gtgcttaccg tctggcagca
    11761 gttgcgggca atggcgaatg ttggcgaaat gaccctgatg tcgcattttg ctgaagcgga
    11821 acatcctgat ggaatttccg gcgcgatggc gcgtattgag caggcggcgg aggggctgga
    11881 gtgtcggcgt tcgttgtcca attcggcggc gactctgtgg cacccggaag cgcattttga
    11941 ctgggttcgg cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat
    12001 cgccaatacc ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac
    12061 gctaaaagcg ggcgagcgtg tgggctacgg cggtcgctat actgcgcgcg atgaacagcg
    12121 aatcggcatt gtcgccgcag gatacgccga cggttatccg cgccatgcgc ctaccggtgc
    12181 tcctgtttta gtggacggcg tgcgcaccat gacggtgggg accgtctcga tggatatgct
    12241 ggcggttgat ttaacgcctt gcccgcaggc ggggattggt acgccggttg agctgtgggg
    12301 caaggagatc aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat
    12361 gtgcgcactg gcgttacgcg tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg
    12421 aggcagcgtc ttctggttgc gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt
    12481 taatgtgaca gagccattgc ccatgatagt gtccattaaa agaatggaca ctatttcccc
    12541 ggaacctgaa ctcgccgcac aggcgttcta cataaaacgc ttacgcttca ttgttgactc
    12601 ggagtcgact tcagataaat cacgcttcac ccttgatgca gattcgggtt catgttcaga
    12661 ttgttgatta acaggctgcg agtcctgttg tctttccaga ctcaacaggc ccaactgttg
    12721 atccaggagc tccaggtcct cctgtacctt cacgcgctcc aggagttccg ggtcatccag
    12781 gaactccaga tccataaggt cctccagctc ctcgagtcct tccaggtcaa cctagccttc
    12841 cttgtgcaaa aagtccaaaa ggtccttcag gccctgctgt tttttcaggc cctcgacggc
    12901 tttgagcagc gtcggtaaat tgtctttgtc ccacgggtta ccattacagt caaacacctt
    12961 caggctctgg ggctgcggca cctgtaccgt tttaaacttg ttttctccac agtagtaaat
    13021 tattagtttg ggcggcagcc gtggtgaaga gatcaaatcg ttactagaac agtcaaatac
    13081 tttcaggcga gtcaagttct ccagcatctc atcaggtatt gagttaatat tgttgccggc
    13141 acaggatagt ttatacaggt actggggcat cgacggcggt aaaaattgca agaagttttc
    13201 agaacagtta atcactctca ggttagtcga cagctcaggc agccagataa tactgttttc
    13261 atagcacttc agtaccatca gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt
    13321 atctgtacag tcaagcacca gcagccactt tggcatcacc ttcggtaacg cggtcaacac
    13381 gtttttactg cagtaaaatt caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa
    13441 tcccaggttg ctaagattga gcatactccg tcttgtaatg agacattcct tcattctgcg
    13501 caatgcttct gcccggtttt caatccggct tgctggcagg ttttcttcca ggatattttc
    13561 cttgatttct tccagacttt ctttcaggtc ttcttgggga atttctgact gtttgttttg
    13621 ctggttttct tccaggattt cgtacaggtt tttttgcagg ttttcttgca aaccatcctg
    13681 ctgtatagcc cattcgcacc atttggcgac catcatgaac tcgttatgtt gctgattcag
    13741 acgagtcccc aacgtgatga tggtattgtt gtccacctgt atgttcactt tgcccgcgat
    13801 actgaggtca atgatctgcc agggggcatc accccagctg aatttttgcg ggctgagata
    13861 cttcatgttc tgcaaaattt gattcttgag aagagccgcc ctggcgggcg tcgtacgcct
    13921 gggttaacgg aagatggggg aatggcgggg gcgaatgggc agatgtcttt aaagctagcc
    13981 ataatgctac gtttccttga aataaatatc ctctggcagc gccggagacg tgaatgtggt
    14041 caccgcaaag gagccacagg acgtcatttg cgctgatatc actactcatc gttgactttt
    14101 ttcctggtca gaaatgccgt gtcgggtctg gtacatgctg tattacggcc taggtagctc
    14161 tgacgtatta aagaaaatac gagagtgaac gacccggaaa taatcgttat taagcaaagg
    14221 aataactata agaaatcgct catgcatccg gtgctacagg cggatgaaac gcctattgtg
    14281 tgggctatgt cagtgcggca ggttgcgtgg gggaatatgt tcaggcgatg tcaggcaggg
    14341 gcccctgatg gttgaccggt aatgcggggc tggtttttat tacctgttac agtcggaaaa
    14401 agcccttacg ctgactttct tgagccggtg ttttgagttc attagccaga gcctgcaatg
    14461 gtttgtcatc cataatttaa ccttcttctg atgttgggat gagcatatca aaatcaggaa
    14521 atgacaagtt tattacaggc tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat
    14581 gctgcattaa atttaagatt cagcttcctc ttcagccacc cgaacgccaa tcttcaacac
    14641 ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc
    14701 gacaaccggt gccgcgccta gcaactgctg gacaatttca cccagcgttt gctgtttatc
    14761 acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct
    14821 ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg gcgactggct
    14881 gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca atacatcgcc
    14941 ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagggctgc
    15001 aatacgcgtc tcttttggca tatgcaaatc gcgcagtgcc gcacctacac accatttatc
    15061 agcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac
    15121 gcgtgacacc ggacgtccga cgggcggaac caccactttg gcttttttcg ccgcccacga
    15181 gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa
    15241 cagtcgcgca ttctccagcc ccgccatcat cgggaacacg gctaggatga ttggcaccgc
    15301 gccgcgtaat cccacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaagg
    15361 gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat
    15421 gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag
    15481 gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg
    15541 gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct
    15601 gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg
    15661 atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc
    15721 aagaccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact
    15781 gacactgctt tcatgctgct ggatcattgc aatcagggta atcgtcagaa agaccgccat
    15841 tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt
    15901 accacccagc agagaaaaga ccgctgcagc atcggtggag ccgacgatag cgccgattaa
    15961 taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga
    16021 ggtgataagt acgcccagcg tcgccagcga cagcgccggt cctaacgcca cacgaaagga
    16081 actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagcgcca ggttacttac
    16141 catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcccgccag
    16201 catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact
    16261 aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt
    16321 ggcatccaat ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta
    16381 gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag
    16441 gatgaccgct aatcgtcaac tgagcgcctt cccgggtgtt attcagaatg gcatgtgcgc
    16501 ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa
    16561 tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat
    16621 cattgggcgt gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc
    16681 ccgcatgata gagctgcaac agcatacgtt cgacgcggaa agggtgcacg ttaatatctt
    16741 ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca
    16801 gcatcgcaag attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt
    16861 gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc tcggtaaagg
    16921 cgttcagttc atccgcacca aagtttgcta ccagcatggg gccgctaaag gtgatgacat
    16981 tgccttgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagta
    17041 acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac
    17101 tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa
    17161 gggaattgag atcttccaga cgctccgttt ccgtacccgc aaaacgctca caacgacggg
    17221 caataacctc gacgttattg acttgatgcc ccgcgtcggt aaggcgctgg ataccacgca
    17281 gcgcggcgtg ctgtttaatg ctgtaacccg atggggcaat taagtgaaac agagacatgg
    17341 caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc
    17401 acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt
    17461 tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa
    17521 cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc
    17581 agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa
    17641 tccgaacgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc
    17701 cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa
    17761 aaatccagag cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc
    17821 gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa
    17881 cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat
    17941 caacgatttg gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca
    18001 ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc
    18061 actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc
    18121 cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt
    18181 tcattcttgc catcaatcac tttgcgctcg ctggtggaga ttaactgggc gtcaaagtga
    18241 aaaacacccc attgccccat gttgacttca atttgagcga agcgcatacc ttcttgtaat
    18301 tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacaag
    18361 cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg cggatggagt
    18421 ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg
    18481 gtaataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg
    18541 actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg
    18601 ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtatt
    18661 ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc tttgtgcaaa
    18721 tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga
    18781 ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca tagttaacaa
    18841 aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc cgacaaacgc
    18901 ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt tcttgcgacc
    18961 gattcatcga aacgagggcc tgaacatggg aattattgcc tggattattt ttggcctgat
    19021 agccggcatt atcggcaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac
    19081 ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat
    19141 tgacggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg tgggcgctat
    19201 cctggtgctg ggcgtattcc gcctcctgcg aagagaataa gattttcata aggcggatag
    19261 cgatacagat gccgctatcc gctttcacat cagaacgtat actcgacacc tgcccagaaa
    19321 ttgcgacctt cttcaataaa cccttcactg taggcatatt gggtatcaaa caggttattg
    19381 accgacgcat tgacgctgaa gccatgacct aaggtgtaat cggctcgaat gtgggtcacc
    19441 gcaaaaccgg cggctttttg tgaaccgtca ctgttgctgt agctggagga acgcgcctct
    19501 tccgacagcg ttacgcttaa cggctcccat ggtttgagtg tcatccatgc ggtcattgtc
    19561 tgcgttggaa gatcggttat cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga
    19621 gcgtagctca atcctacatc cagtatattg ctgattttcc ccttaatacc ggcatccaga
    19681 ccgctgtaat ccaccgtgcc gctgttctga ttttgaatgg tatcggcatt gatattgtgc
    19741 gagaggatgg catcactcac ccggttatag taaacgctga cctcaaatcc ccagtcgcgc
    19801 gtgaaggcac cattccaggt taaatccacc ccgcgagcgc gttccggctt gagctgcggg
    19861 ttaacaatcg ctatctggtt atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc
    19921 ggaaagcgtg tgcggtcata gtacgaaagc gccagagtgt cttcattggc aaagtggtat
    19981 ttccccatca cctgccagtt aaaagctgac tgattgttgt cgtcataatg ggtgatgctg
    20041 ccatctttct cgtgtttttt cgcttctacg ctatcgcgcc agtcatagct gattcccgcc
    20101 acgacatcga cattatcagc agcagcccat tgatattcac tggcgagcga ccaggtacga
    20161 tcttcatagc gatcgtaagc ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc
    20221 cagttaacgg caaacgacag cagatcgttt tcacgcacat cggctgccag ttgtaatccg
    20281 gcaccgtcgc tgtaatcgga ataatggctg tagctgcctt ttttattttt caaatcagcc
    20341 agcgagttgt acatcatcag cgtattttca aaggtgtcgc gatacagccg acttttcagg
    20401 gtaaaacggt cgtttagttg ggtcgctccc tgataataaa aactttcttt gtcatactct
    20461 ggccactgcc agtagcgtga tttttgacca caatttccgc tgtatggcgg gttatctttt
    20521 tcaccatcct gcttaatgta agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg
    20581 agcttcacaa tgccgcgttt atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct
    20641 gcaatatcat tatttacacc atgcggcagg ccgagaaaat cctgctttag ctggctaccg
    20701 ctgacttgca aatacccaag atcgctgctg gcggcaaatg aagcatgcat atcgtaggca
    20761 ttgtcctgac tacggctcca tccctggcga tatcccagac ttgcttcttc agggtggtga
    20821 tattaatggc tccgcccatc tgattaggcc catgaagcag cgacgaatac cctttggaaa
    20881 cttcgactgc ccccagattg ttggtcagaa tccgcgccag atcgaggttg ccgtcatagg
    20941 ggacataaat gggcacaccg tcgaaataga ccggcacctg ccgactatca aagccacgaa
    21001 cttttacctg ttcttcgttg cggctgcctg acttttgcag caccacgccg gggacgacac
    21061 ttaatgcctg agcgacattc tgtttatcca aggccttcat ggtgggttga tcgagaacgg
    21121 tggtcgtcgt cgatgatacc ggactggacc atacggtcag cgtgtcgctc tcctgagccg
    21181 ctacggcctg ctgggtaaag gcaagtgata atgctgtgca gagataacgt tttttgagtc
    21241 tcatagaata aattccgttt aacaaagaat taaatagagg ctgtgggttg cagactgact
    21301 ttaatgtcag ctggtgccgc gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag
    21361 tcgaggtaat ttttgagtgt cgtcagatta attccgccgg tcagagccag cgtgcagtga
    21421 ggagccagcg acggcgctat ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg
    21481 agctgaagta cgtctggttg tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct
    21541 tcaacaacaa ttttcttctc gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag
    21601 ccctgattgt catgaagaaa atggcggtgg ttggcaaaca ataatatggt ttccgcacat
    21661 ccggcacgat gaattagtcc tccggcagcc agaattgcct gcgaagccag taagcgggtg
    21721 cccggaattg cttttcgggt gcaggcgata ttgccgtcag gataacgttc acgaagcaat
    21781 gccagcattt gagcgagata atcagaaaca ccgcaactcc actccagcac attctggact
    21841 gccttccatc cctgatgaag tgctgcggca ttaccctgcg cacggattag ccgttgaccc
    21901 gcgttcgctt gtgaaccgtc gctgaccgcg tcatcaatag ttaatcccag cgtagttaac
    21961 atcttacacg cgacagaaac gccgctgacg cagccgccct gacggtgaaa aaacgctata
    22021 tagccaggct ggtgtccaat atttaatgct cgcgtggtca ggtcacctcc ctggatatct
    22081 tccagcagta acgcatcgat ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg
    22141 gtacgctatc ccaccagatg agcgcccagt cgcggctggc aggggcgagg gcacgagcat
    22201 cttgttgttg ataccagtga tataaccgct cacgctcatc gtcgttaagc gcgccaagac
    22261 tccagctgac gttctgctca aaacgttccc aggtgctgtt atcctgttgg caattttgtc
    22321 cacggataaa atcaacgtgg gcataaattc ccatctgata caggacgttg agcgcaaaga
    22381 tatagttggg taattcgatc acttcccgac cagccgcccg ctgaatggct ggagagacga
    22441 acgaggtgct gaccagatga gttgtataga cgcgaagccg tgcctgatta ttcagtttgc
    22501 tcattgcatg gcgcatatca gccaccagag tggagcggga agccacggca atatcgcagc
    22561 gtggcaggtc actccagtcc tcttcccagg cgcgctggat ccagtggaca ttgtcagcct
    22621 tcagtgcggc ggcgcggcgg gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat
    22681 aaaccgtagt gagtttgtca gccagcgcca gcgatacggt gcctggtccg cagcccatat
    22741 cgaataaggt ctgagcgcct tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt
    22801 ccgttggcga ggcgcaattt tccgccattt tctccgcccg ttgatcccag tgatcgggcg
    22861 ttttttctgt gcggtgagcc agtttgagtt gctgcagata aaggtctgca aaatcaatat
    22921 catcaatgag catgattgtt ccttagcggc taattgcaga gaggtgatga tgaatttggt
    22981 cggcagaaac ccgatacagg gccgccagtt tgttcgttgt taactgttcg gtcggtaagc
    23041 cttgtgttac tcgtccatct ggctctactt ggataatgct gtcggcaatg gcgttggcat
    23101 ggagcgtatg gtgagtcgac attaacattg tcatgccgtt atttttgagc tgcgccagcg
    23161 tgtccagaag ttggatctga tgaccgaaat cgaggctgga ggcgggctca tccagtaaca
    23221 acaagcgcgg ttgttggacg agtgcacggg cgattaaagc cagctgacgt tcaccgccgc
    23281 taagcgtatt ccagcgtcgt gtcgccagat gcaaaatgcc cagcttttcc agttgctccg
    23341 tggctttcag gcgttcttgc ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc
    23401 ccatcaacac catatccagc acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc
    23461 aggcaatagc ctgtgcgcgc tggcgatggc tgagtgtctg cacagagagg ctatccagta
    23521 ggatctcgcc ttttaataac ggtataacgc caagaatgga gcgcatgagt gtggttttgc
    23581 cgcaaccatt cgcgccaagc agacaacaaa ttgttccttg cgacagggtg aaggacacgt
    23641 cgcgtagcac tggatggcgg gagtaaccca gagacgcatg acgaacggca agcaaagtca
    23701 ctggcgacct ccgcgtaaca acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag
    23761 aatgccaagt gggatttctg ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag
    23821 cagaatagcg ccagtacaca ttgccatagg caacagctgc tgatggttat gaccggtcaa
    23881 caagcgggca atgtgcggaa cgaccagacc gatccaacca attatgcccg ctattgctac
    23941 cgtgctggcg gtgattagtg tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt
    24001 cagtcccagc gaacgggctt cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag
    24061 aaaaaacagg ggcaaggagc ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa
    24121 ggtcacggtg gacagtcccc ccagcaacca gaaggtaatt gacggtaact gggtgtaagg
    24181 atcggccagc gttttaatta gcgatatccc cgctccgcac aacgttccta acgcaatgcc
    24241 taccagcacc agggttaaga tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac
    24301 gcccgcgacc accatcagcc cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa
    24361 tccccacagg attgccgcgc aggcaccaag tccggcaccg gcggagacgc ctaaaatatc
    24421 cggtgaaact aagggattac gcaacattcc ctgataggtg gttccggctc ctgccagcgc
    24481 tgcgcccagt aacagcgcag caagaatacg cggcacgcgg atttgccaga agacaatttg
    24541 ttcctgcaca gactcatttt cttgacgaag aattagcgcc agcagttgct gcatatcgag
    24601 atgataagcc ccacttacgg cggcaaaagt gatactgata gctgccagca gcaaaataca
    24661 tccgtttacg attctcatta gctcgccacc aatttttgat agtcggcatc actgagtgaa
    24721 caatgccaga acagttgggc gtaatgctgc atgtcacttt taaactggcg attgatggcg
    24781 ggatccagcc aggcgtgaag tctgcgtaat cccagaagac ggttgattcc cggcggggca
    24841 tcgagccagc caaagggcaa gccacttaaa aataagatgc gttgttccgc aacggctttc
    24901 acgccctgcc agagaggatc acgacgaata aaatctgcag taacggcctc ctgaaccaga
    24961 ataatatccg gctgccagcg caggagattt tccatggaaa cctgtgtcag gccgtggcga
    25021 tcggctattt gcgcgacgtt gtgcaaacct aataattccg ccgcctcggt atgcaacgaa
    25081 ccctgtaagc ccgtttccag ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg
    25141 gcgggtgagg tggcgaatgc ctgagcttca ccaacgaagt ggctggcgag attggcttgt
    25201 tctgcggcgc ggtgctcttc gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat
    25261 tgcgccagtt ttccgttaag caataaccag gggatttgtg tctgttcgct aacctgccgt
    25321 gcctgggaga tcaaggtttc atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac
    25381 gccataagcc cttccagcga tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt
    25441 aactggcgaa tgtgctctgg caatggaatt aatgcctgac gggcaaaatc aaaagaggaa
    25501 aaaccgacca ttttttctgg cgcgacagcc agcaagagca aatcggcggg cgcgcctgcg
    25561 ctgacgatgc gtgtaatgtt ttgggcttcg ggaagagatt gcgaagcaaa acgattaact
    25621 gtttgtgaaa aggacggtag agactgcagg agcagggtag atgcaagtgc ctgcgcaaac
    25681 gtccgtctgg ttatgggcat ggtgaacatc ctgaaaagta aaatcgatat atactttcat
    25741 atatatcgat aaaaatcatg ttcaatatca aagtttagct attgaaaaca tgaagatgtg
    25801 acgacttccg caagcctgtt gaccctctct atcattaaaa atgtatttat caggtggcta
    25861 atattattta aatcatttaa ggctaaatta tttaaatatg aatgacatta ttgttttttt
    25921 atcgtccacg gttacgcgac gttgaagtta cttgttttat aatgagcgat ttttgatatc
    25981 tcgccgagaa aattatcagc attctctcgt ttcagtgtcg tgaatatact gttaacggag
    26041 gtgaaaagaa tgctgggggt tatacaaaac ctatgttgtt gtcgtccgga ttcaccgctt
    26101 gctgatagta agcagcgtaa ccagaagtta ccgcaacaag actttgagga ttttaagaac
    26161 gacgttatta atgtcattaa caacaatgaa gatatttatt ttcttagaga gagtaattta
    26221 ctgaatgcgt ttattcggac gtgcaaaaaa agagatgata acagttacgg aaatatgata
    26281 tttacgcttt attttgataa cgatatctat gggaacagaa ttatcaaagc atcgctgaac
    26341 ggcggagagt tgcagttatt aagcacaggg t
//
LOCUS       DAJTRH010000054        25253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTRH010000054 DAJTRH010000000
VERSION     DAJTRH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO339_002386"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO339_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5926991.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="OO339_002387"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="OO339_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5926992.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="OO339_002388"
     CDS             2630..3391
                     /locus_tag="OO339_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5926993.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="OO339_002389"
     CDS             complement(3442..4326)
                     /locus_tag="OO339_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5926994.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO339_002390"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO339_002390"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5926995.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="OO339_002391"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="OO339_002391"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5926996.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="OO339_002392"
     CDS             6202..6474
                     /locus_tag="OO339_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5926997.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="OO339_002393"
     CDS             6844..7920
                     /locus_tag="OO339_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5926998.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="OO339_002394"
     CDS             7947..9326
                     /locus_tag="OO339_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5926999.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="OO339_002395"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="OO339_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="OO339_002396"
     CDS             10409..11245
                     /locus_tag="OO339_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5927000.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="OO339_002397"
     CDS             11250..12197
                     /locus_tag="OO339_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5927001.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="OO339_002398"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="OO339_002398"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5927002.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="OO339_002399"
     CDS             13324..14082
                     /locus_tag="OO339_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5927003.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="OO339_002400"
     CDS             14200..15558
                     /locus_tag="OO339_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5927004.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO339_002401"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO339_002401"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5927005.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO339_002402"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO339_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5927006.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO339_002403"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO339_002403"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5927007.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="OO339_002404"
     CDS             19503..20054
                     /locus_tag="OO339_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5927008.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="OO339_002405"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="OO339_002405"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5927009.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="OO339_002406"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="OO339_002406"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5927010.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="OO339_002407"
     CDS             23307..24347
                     /locus_tag="OO339_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5927011.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..>25253)
                     /locus_tag="OO339_002408"
     CDS             complement(24464..>25253)
                     /locus_tag="OO339_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5927012.1"
                     /translation="LSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMG
                     FEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKA
                     SRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGVKAVAE
                     GAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLH
                     HIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT         6421 a         5850 c         6528 g         6454 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatattg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgtcttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agc
//
LOCUS       DAJTRH010000055        24193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTRH010000055 DAJTRH010000000
VERSION     DAJTRH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..518
                     /gene="tnpA"
                     /locus_tag="OO339_002409"
                     /pseudo
     CDS             1..518
                     /gene="tnpA"
                     /locus_tag="OO339_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            846..1928
                     /locus_tag="OO339_002410"
     CDS             846..1928
                     /locus_tag="OO339_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5927013.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(2417..3215)
                     /locus_tag="OO339_002411"
                     /pseudo
     CDS             complement(2417..3215)
                     /locus_tag="OO339_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3225..3776)
                     /gene="ybcL"
                     /locus_tag="OO339_002412"
     CDS             complement(3225..3776)
                     /gene="ybcL"
                     /locus_tag="OO339_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5927014.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3945..4277)
                     /gene="emrE"
                     /locus_tag="OO339_002413"
     CDS             complement(3945..4277)
                     /gene="emrE"
                     /locus_tag="OO339_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5927015.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(4622..4936)
                     /gene="fliE"
                     /locus_tag="OO339_002414"
     CDS             complement(4622..4936)
                     /gene="fliE"
                     /locus_tag="OO339_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5927016.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5150..6809
                     /gene="fliF"
                     /locus_tag="OO339_002415"
                     /pseudo
     CDS             5150..6809
                     /gene="fliF"
                     /locus_tag="OO339_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            6802..7797
                     /gene="fliG"
                     /locus_tag="OO339_002416"
     CDS             6802..7797
                     /gene="fliG"
                     /locus_tag="OO339_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5927017.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7790..8476
                     /gene="fliH"
                     /locus_tag="OO339_002417"
     CDS             7790..8476
                     /gene="fliH"
                     /locus_tag="OO339_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5927018.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            8476..9849
                     /gene="fliI"
                     /locus_tag="OO339_002418"
     CDS             8476..9849
                     /gene="fliI"
                     /locus_tag="OO339_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5927019.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9871..10212
                     /gene="fliJ"
                     /locus_tag="OO339_002419"
                     /pseudo
     CDS             9871..10212
                     /gene="fliJ"
                     /locus_tag="OO339_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10209..11336
                     /gene="fliK"
                     /locus_tag="OO339_002420"
     CDS             10209..11336
                     /gene="fliK"
                     /locus_tag="OO339_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5927020.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            11441..11905
                     /gene="fliL"
                     /locus_tag="OO339_002421"
     CDS             11441..11905
                     /gene="fliL"
                     /locus_tag="OO339_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5927021.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11910..12914
                     /gene="fliM"
                     /locus_tag="OO339_002422"
     CDS             11910..12914
                     /gene="fliM"
                     /locus_tag="OO339_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5927022.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12911..13324
                     /gene="fliN"
                     /locus_tag="OO339_002423"
     CDS             12911..13324
                     /gene="fliN"
                     /locus_tag="OO339_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5927023.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            13327..13692
                     /gene="fliO"
                     /locus_tag="OO339_002424"
     CDS             13327..13692
                     /gene="fliO"
                     /locus_tag="OO339_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5927024.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13692..14429
                     /gene="fliP"
                     /locus_tag="OO339_002425"
     CDS             13692..14429
                     /gene="fliP"
                     /locus_tag="OO339_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5927025.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            14439..14708
                     /gene="fliQ"
                     /locus_tag="OO339_002426"
     CDS             14439..14708
                     /gene="fliQ"
                     /locus_tag="OO339_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5927026.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14717..15502
                     /gene="fliR"
                     /locus_tag="OO339_002427"
     CDS             14717..15502
                     /gene="fliR"
                     /locus_tag="OO339_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5927027.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15787..16410
                     /gene="rcsA"
                     /locus_tag="OO339_002428"
     CDS             15787..16410
                     /gene="rcsA"
                     /locus_tag="OO339_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5927028.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(16454..16642)
                     /gene="dsrB"
                     /locus_tag="OO339_002429"
     CDS             complement(16454..16642)
                     /gene="dsrB"
                     /locus_tag="OO339_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5927029.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16805..17032
                     /gene="yodD"
                     /locus_tag="OO339_002430"
     CDS             16805..17032
                     /gene="yodD"
                     /locus_tag="OO339_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5927030.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            17330..18145
                     /locus_tag="OO339_002431"
                     /pseudo
     CDS             17330..18145
                     /locus_tag="OO339_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18142..19836)
                     /gene="yedQ"
                     /locus_tag="OO339_002432"
                     /pseudo
     CDS             complement(18142..19836)
                     /gene="yedQ"
                     /locus_tag="OO339_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20007..20189)
                     /locus_tag="OO339_002433"
     CDS             complement(20007..20189)
                     /locus_tag="OO339_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5927031.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20268..21185)
                     /locus_tag="OO339_002434"
     CDS             complement(20268..21185)
                     /locus_tag="OO339_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5927032.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            21358..22278
                     /gene="yedA"
                     /locus_tag="OO339_002435"
     CDS             21358..22278
                     /gene="yedA"
                     /locus_tag="OO339_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5927033.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22267..22737)
                     /gene="vsr"
                     /locus_tag="OO339_002436"
     CDS             complement(22267..22737)
                     /gene="vsr"
                     /locus_tag="OO339_002436"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5927034.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22718..24136)
                     /gene="dcm"
                     /locus_tag="OO339_002437"
     CDS             complement(22718..24136)
                     /gene="dcm"
                     /locus_tag="OO339_002437"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5927035.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
BASE COUNT         6119 a         6161 c         5974 g         5939 t
ORIGIN      
        1 ttattttgaa gctgaactgg ttgaaatgga tggcgaacca gatcacgtcc atctgttaat
       61 taactatccc cccaaactgg cgatatccag tctggtaaac aaacagcctc aaaggcgtat
      121 cgggtaggtt actgcgacgg gatcgaccag atattgctgc caggtattac cgcaaagatg
      181 ttttgtggcg tcccggctat tttgccagta gctgcggagg tgcgccaata tccgtcatcc
      241 gccaatacat tgaacaacag caaacacctg gtcagatgaa aaaacgagcc ttatatcccc
      301 gccctgaagg acgggctttt acggtgcact ggataagcct ggagattatc tacacctgca
      361 tggacatcat tgtactatgc cccgtacatg ctccatcaca tcctcaacat caaagtatta
      421 gagccttgca aaccattatg cgtctcagtt tgtctcatct atcttacgca aaaaaaatag
      481 aactaatatc aagcggcgct atattgcgta ttaaataaaa ccacctcaat tacattaata
      541 aatctaaata aatatcatgc aaataatttc catggttatt tttaggctat ttaaattaaa
      601 aaaataaatt atgagtcaat cataaataac aacctttaat ataatttgac taccaaattt
      661 atattcgttc acaaaatagt catgcattgt gtgcaataga aacagttcag ataaaaatag
      721 ggatctactt cacaatttaa atgagaaact aaaacttaca tcttgaaata atctcattga
      781 ttggatgaat atttgtagtg cagtgacatc atttttaaat aatagttcaa ataaaagggc
      841 tcacgatgaa aaaattaaca gtggcaattt ctgctgtagc tgcatcagta ctgatggcga
      901 tgtctgctca ggcagcagag atttacaata aagacagtaa caagctggat ctgtacggaa
      961 aagttaatgc taagcactac ttctcttcta acgatgcaga tgatggcgac acaacttatg
     1021 ttcgtctggg cttcaaaggc gaaactcaaa tcaacgatca actgactggt ttcggtcagt
     1081 gggaatatga attcaaaggc aaccgtgctg aatctcaagg ttcttccaaa gataaaaccc
     1141 gtcttgcctt cgctggcctg aaattcggtg actacggctc catcgattat ggccgtaact
     1201 acggtgtagc atacgatatc ggtgcatgga ccgacgttct gccagaattc ggtggcgata
     1261 cctggaccca aacagatgtg ttcatgactg gtcgcaccac aggtgttgca acctatcgta
     1321 acaacgactt ctttggtctg gttgatggtc tgaactttgc tgctcagtat cagggtaaaa
     1381 atgaccgcac tgacgtaact gaagctaacg gtgatggttt cggtttctcc actacttatg
     1441 agtatgaagg attcggtgta ggtgcaacct atgctaaatc tgaccgcact aatgatcagg
     1501 ttatctacgg taacaacagc ctgaatgcat ctggtcaaaa tgctgaagta tgggcagctg
     1561 gtctgaaata tgatgcgaac aacatctatc tggctaccac ctattctgaa acccagaaca
     1621 tgactgtttt tggtaataac catattgcca acaaagcaca aaacttcgaa gtagttgcac
     1681 aatatcagtt cgacttcggt ctgcgtccgt ccgttgctta cctgcaatct aaaggaaaag
     1741 acttgggtgc gtggggtgat caggacctga ttgaatatat tgatgtaggt gcaacctatt
     1801 acttcaacaa aaatatgtcc acttttgttg attacaaaat caacctgatt gataagagcg
     1861 atttcacgaa agcatctggc gttgctaccg atgatatcgt tgctgtagga ttggtttacc
     1921 agttctaatc tgattactca aagatatgtt gtgggaggct ttgcctcccc aacatataag
     1981 tggcgctctc aagccacttt ccttagaagc actatcttgc ttctgactat ataaattacc
     2041 ctttattttc tgggacgttt caacgcccca tttttaataa tactcagtga acaattaacg
     2101 tatcaattag atttaataac aacgatatcc gtacctaacc tgatatctaa tgtcataagc
     2161 atccctttaa ttatcccctc aacctcttgt aaatttccca ctcttgagct ccacatagcc
     2221 caaacgccgg atgcaaaaaa aatcatacgt gcatctctga aattgtgatg gaggaagtga
     2281 gtatcaatta tatgtgctta taaacgttcc cgattggcac agaagtcacc ggattgttca
     2341 ggctccaata ggttgagtat gtcgtattta ttttccaaaa taaaaatcga tgctgttgct
     2401 ccttaataaa atacttttac tctctggacg cttttcttag ctctcttggt gttatattaa
     2461 aacgattatg gaacctttca gtaaaacgag aaggacactt gtaaccattt tccctggcaa
     2521 tctcgctgat gggttttact gtcgtttgta tagcaggaca acgcattatt taacctcacg
     2581 tcgtccaata tactttgaaa acttattccc tcgcttgcca gacggcgatg caatgtggaa
     2641 acagaaatgt agagatatct cgctaccttg tttgctgtcc attttgttct aggttcggat
     2701 agcattaggt tataacaacg acttattaat gattgtttac tatctgataa aagtaaacaa
     2761 ttaacatgat gtgctcctaa tgaaagtaga acgcccattg ctaagtgttc ctgaatttgc
     2821 gttgagaagc ctcgggaaat agatgttttt agttgctccc aacaatatat taactcgggg
     2881 ttctgaggta agcagaaacc tgtcgtgtta cgaatttgat cagttatcgc ataaaatttt
     2941 tgaaactttt caattagatc aatgggataa taaagcattt ctgcaagata aagacctgct
     3001 tctggataat tcacaatata aaattcatat ccacagggaa ataatattat ctgattatta
     3061 tcaacagtta gactatgcgt ctcccaattg ataacttttt ttccctgacg gatacgacat
     3121 aaagctggca taagaggctt aaccctgtga atctcatgat gtttatgcat ccgtatttct
     3181 tcaatcttta ggttagactt atctcttgcc agcatactct taccctattt tatctcataa
     3241 actggtgtta tctcagcggt tgcaatttta ttggcattaa gcatataacc aactaacgct
     3301 ccgctggaat tagaatccac aggaatctta tcagttttta gagcccatac tttaaactgg
     3361 taatgatggg gtttatctcc tttgggagga catgctccac caaatcctgc ataaccaaaa
     3421 tcatttcggc cttgaacagc accagtcgga agttttgtac catcacgtct tcctgcatca
     3481 gtgggcaaat atgttacagt tgctggaata ttagcaacag tccagtgcca ccatccactg
     3541 cctgtaggag catctggatc atacacagtt acggcaaagc ttttggtgcc ttcaggagca
     3601 ccagaccagg ttaatgaggg cgatgtatta ccaccttcac acccaaatcc agaaaataca
     3661 tgagacgttg taagttgctc tcctgttttt atttcattac tagtgacctg aaatgctgcg
     3721 gcctgcgcag aaaatgttat gaatgccaat acacttgaaa cgataagttt tttcataaaa
     3781 acctcattgt tgtgacctat cgttatttta tttgatgtgc cttgatctca ttatgcataa
     3841 aggcgcaatg atcgcgcaaa tgcaatcata attataaagg tgccccgaac attagccata
     3901 atatgcacaa atcagattcc tgttatctca aggagaatta ttttttaatg cggtgcactt
     3961 cgtgacaaca agttaatgac taacacaccg gcacaaatca acatcatgcc tatgatagct
     4021 ggcaggtcca gtcgttgacc aaaaaatccc caggaaagca aactaatcag aacgataccg
     4081 actcctgacc agatagcata agcaatccct gtaggtatat aagccagcgt ctgagctaat
     4141 aaccagaatg atgcacaata gcaaataatt gtgccaacag agggccataa ccgtgtaaac
     4201 ccctctgaaa acttcattaa ggttgtacca atgacctctg caagtattgc gccaccaaga
     4261 taaatataag ggttcatagc atattctttc ctgttcatac tggagagaat tgtactacag
     4321 tttgaacaca actcaccgtt ttcatcctgg tgtatgtgta tccaaggggc tctggcgtgg
     4381 caagagataa aggcaggaac aataatgatc agaaggaacc ccaggctaac gaatgagttc
     4441 actcttcctg ggagcaaagc gaagacaaaa catgttatcg cgtttacgga tcgtggatgg
     4501 cagaaaacaa ccaggatcag gtatccattt taaaccagaa attgagtgag tttgccccat
     4561 ccatgcccca cgctgtggga tcggatataa ttaaacaagc atagtcaaca ggttacacaa
     4621 cctacacctg catggacatc acttcctgat acgccgccac cagcttatta cgcacctgaa
     4681 tccccatttg catagaaact gaggcttttt gcatatcggt catcacatcg tttagcgcca
     4741 cgccgggttc accgagggtg aatttttctg cctgcgtgcg ggcgactgtt tgtgtatcgc
     4801 ttatgcgatc gagcgcggcg tgcagctgcc cggcaaaact aatggtcggt tgcggcagtg
     4861 attcctgcgc acgcgcactc atcgccgtag cctgtaactg gctgataacc ccttcaatcc
     4921 cctgtatcgc tgacattttc gtctcccgga taatttctgg taacaaagcc taccagtaag
     4981 tcaataagac aaaggcgcta aatagcaaca aaaaaactgg tttattggcg gatagaaaaa
     5041 acgaaagcac aaataatggg agcgtcaatt tttcgagtgt gctgacccga gagtgagtct
     5101 tgttccactt tgccaataac gccgtccata atcagccacg aagtgcgcga tgaatgcgac
     5161 tgcagcccag acaaaatctc ttgagtggct taatcgcctg cgtgcgaatc cgaaaattcc
     5221 attgattgtt accggttccg cggcagtggc ggtcatggtc gcactgatcc tgtgggcgaa
     5281 agcccccgac taccgcacat tattcagcaa tctttccgat caggatggtg gcgcaattgt
     5341 cagccaactg acgcaaatga atattcctta ccgcttcagc gaagccagcg gcgctattga
     5401 ggttccggca gataaagttc acgaactgcg cctggcgcaa caaggtttgc ccaaaggcgg
     5461 cgcggtcggt ttcgaactgc tcgatcagga aaagtttggt atcagccagt tcagcgaaca
     5521 ggtgaattat cagcgggcgc tggaaggcga gctttctcgt accatcgaaa ctatcggccc
     5581 ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg tctttattcg tccgtgaaca
     5641 aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac tcgatgaagg
     5701 gcaaattagc gctattgtac atctggtttc cagcgcggtt gctggtctgc cgcctggaaa
     5761 cgtcacgctg gtggatcagg gcgggcatct gttaacccag tccaatacca gcgggcgcga
     5821 tcttaatgac gctcagttga aatatgccag cgatgtcgaa ggccgtattc agcagcgtat
     5881 tgaagcgatc ctgtcaccta ttgttggtaa cggtaatatt cacgcctagg tcacggcgca
     5941 actggacttc gccagtaaag aacaaacgga agaacagtat cgccctaacg gtgatgaatc
     6001 tcatgcggcg cttcgttcac gccagcttaa tgagagcgag caaagcggtt ccggttatcc
     6061 gggcggcgta ccgggggcgt tgtcgaatca accggcacct gcgaataacg cgccaatcag
     6121 cacgcctccg acaaatcaaa ataaccgcca gcagcaggcg agcaccacca gcaatagtgg
     6181 gccgcgtagc acacagcgga atgaaaccag taactacgaa gtcgatcgca ccattcgtca
     6241 taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc gcggtagtgg tgaattacaa
     6301 aaccttgcca gacggtaaac cattgcctct cagcaacgaa cagatgaagc aaattgaagc
     6361 tctgacccgc gaggcgatgg gcttttctga aaaacgcggt gattcgctca atgttgttaa
     6421 ctcgccgttc aatagcagtg acgaaagcgg cggagcactg ccgttttggc aacagcaagt
     6481 gtttatcgat cagttgctgg ctgccggtcg ctggttgctg gttgctggta ctgctggtgg
     6541 cgtggttgct gtggcggaaa gcggtacgcc cgcagctaac acgtcgcgcc gaggcggtga
     6601 agactgtgca gcaacaggcg caggcccgcg aggaagtgga agatgcggtg gaagtccgtc
     6661 tgagcaaaga cgaacaactc caacaacggc gcgctaacca acgactgggg gcagaagtca
     6721 tgagccagcg tatccgtgaa atgtctgata acgatccgcg cgtggtggcg ctggtcattc
     6781 gccagtggat aaataacgat catgagtaac ctgacaggca ccgataaaag cgtcatcctg
     6841 ctgatgacca ttggcgaaga ccgggcggca gaggtgttca agcacctctc ccagcgcgaa
     6901 gtgcaaaccc tgagcgctgc aatggcgaac gtcacgcaga tctccaacaa gcagctaacc
     6961 gatgtgctgg cggagtttga gcaagaagct gaacagtttg ctgcactgaa tatcaacgcc
     7021 aacgattatc tgcgttcggt attggtcaaa gctctgggtg aagaacgtgc cgccagcctg
     7081 ctggaagata ttctcgaaac ccgcgatacc gccagcggta ttgaaacgct caactttatg
     7141 gagccgcaga gcgccgccga tctgattcgc gatgagcatc cgcaaattat cgccaccatt
     7201 ctggtgcatc tgaggcgcgc ccaggccgcc gatattctgg cgttgttcga tgaacgtctg
     7261 cgccacgacg tgatgttgcg tatcgccacc tttggcggcg tgcagccagc cgcgctggcc
     7321 gaactgaccg aagtactgaa tggcttgctc gacggtcaga atctcaagcg cagcaaaatg
     7381 ggcggcgtga gaacggcagc cgaaattatc aacctgatga aaacgcagca ggaagaagcc
     7441 gttattaccg ccgtgcgtga attcgacggc gagctggcgc agaaaatcat cgacgagatg
     7501 ttcctgttcg agaatctggt ggatgtcgat gatcgcagca ttcagcgtct gttgcaggaa
     7561 gtggattccg aatcgctgct gatcgcgctg aaaggggccg agcagccact gcgcgagaaa
     7621 ttcctgcgca atatgtcgca gcgtgccgcc gatattctgc gcgacgatct cgccaagcgt
     7681 ggtccggtgc gtctgtcgca ggtcgaaaac gaacagaaag cgattctgct gattgtgcgc
     7741 cgtcttgccg aaactggcga gatggtgatt ggcagcggcg aggataccta tgtctgataa
     7801 tctgccgtgg aaaacctgga cgccggacga tctcgcgcca ccaccggcag agtttgtgcc
     7861 aatggtcgag tcggaagaaa ccattattga agaggccgaa cccagccttg agcagcaact
     7921 ggcgcaactg caaatgcagg cccacgagca gggttatcag gcggggattg ccgaaggtcg
     7981 ccagcaaggt cacgaacagg gctatcagga aggactggcc caggggctgg aacaaggtct
     8041 ggcagaggcg aaggctcaac aagcgccaat tcatgcccgg atgcagcaac tggtcagcga
     8101 atttcaaact acccttgatg cgcttgatag tgtgatagct tcgcgcctga tgcagatggc
     8161 gctggaggca gcacgtcagg tgatcggtca gacaccaacg gtggataact cggcactgat
     8221 caaacagatc caacagttgt tgcagcaaga accgttattc agcgggaaac cacagctgcg
     8281 cgtacacccg gatgatctgc aacgtgtgga tgatatgctc ggcgcaactt taagtttgca
     8341 tggctggcgc ttgcggggcg atcccaccct ccaccctggc ggatgtaaag tctccgccga
     8401 tgaaggcgat ctcgacgcca gtgtcgccac tcgctggcaa gaactctgcc gtctggcagc
     8461 accaggagtg gtgtaatgac cacgcgcctg actcgctggc taaccacgct ggataacttt
     8521 gaagccaaaa tggcgcagtt gcctgcggta cgtcgctacg ggcgattaac ccgcgctacc
     8581 gggctggtgc tggaagccac cggattacaa ttgccgctcg gcgcaacctg tgtcattgag
     8641 cgccagaacg gcagcgaaac gcacgaagta gaaagcgaag tcgttggctt taacggtcaa
     8701 cggctgtttt taatgccgct ggaggaagtg gaaggtgtcc tgcccggcgc gcgtgtatat
     8761 gccaaaaaca tttcggcaga agggctgcaa agcggcaagc agttgccgct cggtccggcg
     8821 ttattaggtc gtgttctgga cggtagcggt aaaccgctcg atggcctgcc cttccccgat
     8881 acgacggaaa ccggtgcgct gattaccccg ccatttaacc cgttgcaacg tacaccgatt
     8941 gaacatgtgc tggacaccgg cgtgcgccca atcaatgccc tgcttaccgt cggacgcggg
     9001 cagcgtatgg ggctgtttgc cgggtcgggc gttggtaaaa gtgtgctgct ggggatgatg
     9061 gcacgctaca cccgcgccga tgtcattgtc gtgggtttga ttggtgaacg tgggcgcgaa
     9121 gtaaaagatt ttattgagaa tatcctcggt gccgaaggac gtgcacgctc agtggtgatt
     9181 gccgctccgg cggatgtttc tccgctcctg cgaatgcaag gtgccgccta tgccacgcgc
     9241 attgccgaag attttcgcga tcgtggtcag catgtgttgc tgattatgga ctccctcacc
     9301 cgctacgcga tggcccagcg tgaaattgcg ctggcgattg gcgaaccacc tgccactaaa
     9361 ggttatccac cgtcggtgtt tgccaaatta ccggcactgg tcgagcgtgc cggtaatggc
     9421 attagcggcg gcggctcgat taccgcgttt tataccgtgc tcaccgaagg cgatgaccag
     9481 caggacccca ttgctgactc cgcgcgggca atcctcgacg gtcacattgt gctgtctcgc
     9541 cgactggcgg aagccgggca ctatccggct atcgatattg aagcgtcgat cagtcgcgca
     9601 atgacggcgt tgatcagtga gcaacattac gcgcgagtgc gcaccttcaa acagctgttg
     9661 tcgagttttc agcgtaaccg cgatctggtt agcgtcggcg cgtatgccaa aggcagcgat
     9721 ccgatgctcg ataaagccat cgccctgtgg ccgcagctgg agggctattt gcaacaaggc
     9781 atttttgaac gcgcggactg ggaagcgtct ctccaggggc tggagcgtat tttcccgaca
     9841 gtgtcataac ccaggagata acggcagatg gcggaagaac agctcaaaat gttgatcgat
     9901 tatcagaatg aatatcgcaa taacctcaat agcgatatga gtgccgggat gaccagcaac
     9961 cgctggatca actatcagca gtttatccag acgctggaaa aagccattac tcagcatcgc
    10021 cagcaactta atcagtggac gcagaaagtt gacattgccc tgaacagctg gcgagaaaag
    10081 aaacaacgtt tgcaggcctg gcagacactg caggaacggc aatccacggc ggcactgctt
    10141 gcagaaaacc gcctcgatca gaaaaagatg gatgagttcg cccagcgcgc cgccatgagg
    10201 aaacctgaat gattcgctta gcgcccttga ttaccgccga cgttgacacc accacattgc
    10261 ctggcggcaa agccagcgat gctgcacaag attttctcac gttgttgagc gaagcattag
    10321 caggcgagac aacaactgac aaagcggccc cccagttgct ggtagcaaca gataagccca
    10381 cgacaaaagg cgagccgctg gtcagcgata ttcttgccga tgcgcaacaa gcggatttac
    10441 tgatccctgt ggatgaaaca cttcctgtca tcaacgacga acaatccaca tcaacaccgt
    10501 taaccaccgc tcagacgatg acgttggctg cggtggctga caaaaatacg acaaaagacg
    10561 aaaaagcgga tgatcttaat gaagacgtca ccgcaagtct gagcgccctt tttgcgatgt
    10621 tgccgggttt tgacaatacg cccaaagtga ctgatgcacc gtcaaccgtg ttaccggcag
    10681 agaaaccaac gctcttcaca aaactgactt ctgcgcaact cactacagca cagcctgatg
    10741 acgcccccgg cacaccagct cagccattaa caccgctggt agcagaagcc cagagtaaag
    10801 cggaagtcat cagcacgcct tcgccggtga ccgctgacgc cagcccgcta atcactccac
    10861 accagacaca gccactgccc accgtcgccg cgcctgtttt gagtgcaccg ctgggttctc
    10921 acgaatggca acaatcattg agccagcata tttcgctgtt cacccgccag gggcaacaaa
    10981 gtgcagagtt gcgtctgcac ccacaggatt taggtgaagt gcaaatttcc ctcaaagtgg
    11041 atgataacca ggcgcagatc cagatgattt caccgcatca gcacgtacgc gccgccctgg
    11101 aagcagcgct gccggtactg cgtacgcagc tggccgaaag tggcattcag ttagggcaaa
    11161 gcaacattag tggcgaaagc tttagtggtc agcagcaggc cgcttcccag caacagcaaa
    11221 gccaacgcac agcaaaccat gaacctctgg cgggggaaga cgacgatacg cttccggttc
    11281 ccgtctcttt acaagggcgt gtaacaggta acagcggcgt tgatattttc gcctaacgtc
    11341 agaggtagca ccgtaatccg cgtcttttcc ccgctttgtt gcgctcaaga cgcaggataa
    11401 ttagccgata agcagtagcg acacaggaag accgcaacac atgactgatt acgcgataag
    11461 caagaaaagc aagcgatcgc tttggatccc gattctggta ttcattaccc tcgcggcctg
    11521 tgccagcgca ggttacagct actggcattc gcatcaggtt gccgctgacg acaaagcgca
    11581 gcaacgcgtc gtgccctccc cggtcttcta tgcgctggat acctttacgg tcaatttggg
    11641 cgatgcggat cgcgtgcttt atatcggcat aaccctgcga ctgaaagatg aagcaacccg
    11701 ctcgcggctg agtgagtatt tgccggaagt ccgtagtcgc ttgctgttac tgttttcacg
    11761 tcaggatgct gccgtactgg cgacagaaga aggcaagaaa aacctgattg ccgagattaa
    11821 aaccacactt tccaccccgc ttgttgccgg gcaaccgaaa caggatgtca ccgacgtgct
    11881 gtataccgct tttattctgc gataacgaca tgggcgatag tattctttct caagctgaaa
    11941 ttgatgcgct gttgaatggt gacagcgaag tcaaagacga accgacagcc agtattagcg
    12001 gcgaaagtga cattcgtccg tacgatccga atacccaacg acgggttgtg cgcgaacgtt
    12061 tgcaggcgct ggaaatcatt aatgagcgct ttgcccgcca ttttcgtatg gggctgttca
    12121 acctgctgcg ccgtagcccg gacattaccg tcggggccat ccgcattcag ccgtaccatg
    12181 aatttgcccg caacctgccg gtgccgacca acctgaacct aatccacctg aaaccgctac
    12241 gcggcacagg gctggtagtg ttctcgccga gtctggtgtt tatcgccgtg gataacctgt
    12301 ttggcggcga tggacgcttc ccgaccaaag tggaaggtcg cgagtttacc catactgaac
    12361 agcgcgtcat caaccgtatg ttgaagctgg cgctggaagg ctatagcgac gcctggaaag
    12421 cgattaatcc gctggaagtt gagtacgtgc gttcggaaat gcaggtggaa tttaccaata
    12481 tcaccacctc gccgaacgac attgtggtta acacgccgtt ccatgtggag attggcaacc
    12541 tgaccggcga atttaatatc tgcctgccat tcagcatgat cgagccgcta cgggaattgt
    12601 tggttaaccc gccgctggaa aactcgcgta atgaagatca gaactggcgc gataacctgg
    12661 tgcgccaggt gcagcattca cagctggaac tggtggccaa ctttgccgat atctcgctac
    12721 gcctgtcgca gattttaaaa ctgaaacccg gcgacgtcct gccgatagaa aaacccgatc
    12781 gcatcatcgc ccatgttgac ggcgtcccgg ttctgaccag tcagtacggt actctcaacg
    12841 gtcagtatgc gttacggata gaacatttga ttaacccgat tttaaattct ctgaacgagg
    12901 aacagcccaa atgagtgaca tgaataatcc ggccgatgac aacaacggcg caatggacga
    12961 tctgtgggct gaagcgttga gcgagcaaaa atcaaccagc gaaaaaagcg ctgccgacgc
    13021 ggtgttccag caatttggcg gtggtgatgt cagcggaacg ttgcaggata tcgacctgat
    13081 tatggatatt ccggtcaagc tgaccgtcga gctgggccgt acgcggatga ccatcaaaga
    13141 gctgttgcgt ctgacgcaag ggtcagtcgt ggcgctggac ggtctggcgg gcgaaccgct
    13201 ggatattctg atcaacggtt atttaatcgc ccagggcgaa gtggtggttg ttgccgataa
    13261 atatggcgtg cggatcaccg atatcattac cccgtccgag cggatgcgcc gcctgagccg
    13321 ttagtgatga ataaccatgc tactgtgcaa tcttccgcgc cggtttctgc tgagccactg
    13381 ctgcaggtga gcggcgcact catcgccatt attgccctga tcctcgctgc tgcctggctg
    13441 gtaaaacggc tgggatttgc ccctaaacgc actggcgtta acggtctgaa aattagcgcc
    13501 agtgcttcgc tgggcgcgcg tgaacgggtc gtggtggtcg atgtggaaga tgcacggctg
    13561 gtgctcggcg ttaccgcagg gcaaatcaat ctgctgcata aacttccccc ttcagcacca
    13621 acggaagaga taccgcagac cgattttcag tcggtcatga aaaatttgct taagcgtggc
    13681 gggagatcct gatgcgtcgt ttgttttctg tcgcaccggt ccttctctgg ctgattacgc
    13741 ccctcgcctt cgcgcaactg ccgggtatca ccagccagcc gctacctggc ggtggacaaa
    13801 gctggtcgct cccggtgcag acgctggtgt tcatcacctc gttgacgttc attccagcga
    13861 ttttactgat gatgaccagt ttcacccgca tcattattgt ttttggttta ttgcgtaacg
    13921 cgctgggaac gccctccgcg ccacctaacc aggtattgct ggggctggca ctgtttttga
    13981 ccttttttat tatgtcgcca gtgatcgaca aaatttatgt cgatgcgtac cagccattca
    14041 gcgaagagaa aatctcaatg caggaggcgc tggaaaaagg ggcgcagccg ctgcgtgagt
    14101 ttatgctgcg tcagacccgc gaggcagatt tagggttgtt tgccagactg gcgaataccg
    14161 gcccgttgca ggggcctgaa gctgtgccga tgcgcatttt gctcccggcc tacgtgacca
    14221 gcgagttgaa aaccgcattt cagataggct tcacgatttt catccctttt ttgattatcg
    14281 acctggtgat tgccagcgtg ttgatggcat tggggatgat gatggttccc cccgccacca
    14341 ttgctctgcc ctttaaactg atgctgtttg tactggtgga tggctggcaa ttgctggtcg
    14401 gttcgctggc gcagagcttt tacagctaga gaggcaaaat gacacctgaa tcggtcatga
    14461 tgatgggaac tgaagcgatg aaagtcgcgc tggcactggc agccccgctg ttgctggtag
    14521 cgctggtcac gggccttatc atcagtattt tgcaggcagc cacgcagatt aacgaaatga
    14581 cgctgtcgtt tattccgaaa atcatcgccg tatttatcgc catcattatt gccggaccgt
    14641 ggatgctcaa tctgttgctg gattacgtcc gcaccttgtt cactaacctg ccgtatatca
    14701 tcgggtagcc cgtacaatga tgcaggagac aagcgatcaa tggctttcct ggttaagcct
    14761 gtacttctgg ccgttactgc gcgtgctggc gctcatttct accgcgccaa ttctgagcga
    14821 acgcagcgtg ccgaaacggg taaaactggg tctggcaatg atgatcacgt tcgccattgc
    14881 cccatcatta cctgccaacg atgttcctgt tttttcgttc tttgctctgt ggctggccgt
    14941 acagcagatc ctgatcggca ttgcgcttgg ttttaccatg caatttgcct ttgccgctgt
    15001 gcgaaccgct ggcgaaatta tcggtctgca aatggggctg tcatttgcga catttgtcga
    15061 tcctggcagc catctcaata tgcccgtttt agcgcgcatc atggatatgc tggcattact
    15121 gctgttcctg acatttaacg gtcatttatg gttgatttca ctgctggtcg atacctttca
    15181 caccctgccg attggtagcg aaccgttgaa cagcaatgcg tttctggcac ccaccaaagc
    15241 agggagtttg attttcctta acgggctgat gctggcgtta ccgctcatta ctctgttgct
    15301 gaccctgaat ctggcattag gtttactgaa tcgtatggcc ccgcaattat ccatttttgt
    15361 tattggattt ccattaactc tgactgtcgg catctcttta atggcggcat taatgccgtt
    15421 aattgcgcct ttttgcgaac atttattcag tgaaattttt aatttgctgg ctgatattat
    15481 tagtgaatta ccattaatat aattccgtaa cgtttatcat gctaccctaa ggattatccg
    15541 aaaaataata tctgcgaaca tcttccagga tactcctgta gcgaaatatt tgttttaagc
    15601 tcactcacat atcgcaacat ttactttaag acaattccag gcaaattata caacacttta
    15661 cgggatagta agtccgcctg aaaaattgcg agagtggcgc attaggtgac ccatgttgtt
    15721 ccgtttagtc atgatgaaat attcaggtaa ggggaattat cgttacgcat tgagtgaggg
    15781 tatgccatgt caacgattat tatggattta tgtagttaca cccgactagg tttaaccggg
    15841 tatctgttga gtagaggggt taaaaaaaga gaaatcaacg acattgaaac cgttgatgac
    15901 cttgccatag cttgtgattc acagcgtcct tcagtggtgt ttattaatga ggactgtttc
    15961 atccacgatg cttctaacag tcagcgcatc aagcacatca ttaatcaaca tcccaatacg
    16021 ttatttatcg tttttatggc aattgccaat gttcattttg atgaatatct attggtcaga
    16081 aaaaatttat tgatcagttc taaatcgatt aaaccggaat ctctcgacga tatccttggc
    16141 gatattctga aaaaagagac aacgataacc tcgtttttaa atatgccgac gttatcattg
    16201 agccgaaccg aatcgagtat gttgcgaatg tggatggcag gtcagggaac cattcaaatc
    16261 tccgaccaaa tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa tattaaacgt
    16321 aagatcaaaa cgcataataa acaggttatc taccatgtcg tccgactgac ggataatgtg
    16381 actaatggta tttttgtcaa catgcgctaa cacattctga ctggtggttt cccaccagtc
    16441 aggctgaata agattactct gctttctcaa caaagatacc gtcctgatgc cctgcttcat
    16501 taaagaacca gatgccgagc gggtagtctt ccagcgcaac caggtacatt gtgccttcac
    16561 taaactcctc aactgccagt accactccag gacgacgcgg accgccatcc gttttgactg
    16621 ttacccgatc attcaccttc atcgttttcc tcctgtggct ttgtgccagt gtagaacaat
    16681 ttcgtttttc tggcagcgcc aagcgcgcgc gagtgctgat tttctcgacg gtctatactt
    16741 aagagatgcc aggcggactt aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa
    16801 gatgatgaaa accgcaaaag tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt
    16861 cgatgccctg ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa
    16921 cgattctgaa cacgttagcg tcgatggacg tgaatatcat acatggcgtg aattggcgga
    16981 tgccttcgaa ctggatattc atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa
    17041 taaaaaaatc ccgaccctga gggtgtcggg atgaaacttg cttaagcaag aagcacttaa
    17101 aaaattcgtt acaccaggaa atctgatgtg ttcatcacct tatctgcaat ttttttcgct
    17161 gacaagaaaa tattcgcgct atgaatgatt aacgctactt atgaatagaa atgtgacgcg
    17221 cgtcacccct tggtggctct ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt
    17281 atttgtgctg ttttgagaat catcctggta aggggagtac tgcgcgacca tgttttcaat
    17341 tcaacaacca ctactggttt ttagcgatct tgatggcacc ctgctggaca gtcatagtta
    17401 tgactggcaa ccggcagccc cctggctcag ccgtttacac gaagccaata ttcccgtcat
    17461 tctctgtagc agtaaaactt tagcggaaat gctgtacttg caaaaaatgt tggggctaca
    17521 aggcttaccg ctgattgcag agaatggcgc agtgatccag cttgctgaac aatggcagga
    17581 tatagacggt tttccacgca tcatctcagg tattagccat ggcgaaatct gccaggtttt
    17641 aaatacgcta cgcgagaaag aacattttaa attcacgact tttgatgatg tcgacgatgc
    17701 aaccattgcc gaatggacgg gattaagccg tagctaggcg gcgctgacgc agctgcatga
    17761 ggcgtcggta acgctaatct ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg
    17821 tctgaacgaa ctgggcttgc agtttatgca gggtgcgcgc ttctggcacg tactggatgc
    17881 ctctgccgga aaagatcagg ctgccaactg gattatcgcg acctatcaac aattgtcagg
    17941 cagacgccca accacacttg gcctgggcga tgggccaaac gatgcgcctt tactggaggt
    18001 aatggattac gcggtgattg tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga
    18061 ggatccggcc cgcgtctggc gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct
    18121 ggaccatttt ttctccgccc gttaagcgtt atcgctcgcg aatacccgat tgcgcccggc
    18181 ctgttttgcg aggtaaagcc gacggtcggc caatgactgg agttgttcaa aatcataatc
    18241 accggtttcc tcgctgctac ttacccccag cgaggcactg atgcgtatcg ttgtactctt
    18301 ggcgatcagc atctcttttt catttaactt caggcgaata cgttctgcga cctccgcagc
    18361 ctgcgtcaga ttcgcgcctg gcagaatcac acaaaactcc tcaccaccga cccgcccggc
    18421 aacgtcctgc gcacgcaagg aactgctaat taatccggca gcatgagaaa gaacacggtc
    18481 gcccgcctga tgaccaaagc ggtcattaat cgctttaaaa tggtcaagat cgacctgaat
    18541 gacagaaaaa ggatgttggt gcgtctgaca caatttagcg agcggacggg ctttttcgaa
    18601 cagtgcgcca cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga
    18661 gctttgcaga acatacatgt tgctgaccat ccggcgaatc acataccagg agaggagtaa
    18721 catggtggta aagagcgccc acagcagggt taatgcaatg ctgatactgc cgaaatcgcc
    18781 gcgcacgcct tcgcttagcg tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg
    18841 ttcccagcta acatagcgac tgtccatacg aatgccgccc cgcgtgtcat gttccattgc
    18901 ctgcgccagc aaggccagtt cacgaggatc aaaaatattc cctgttggat ggtcaggatt
    18961 ggaagaggtc aaaaatctca gcttactgtc atagagctga tactcaccat cgaggttttt
    19021 atcgatggcg tttcttaaaa attgctgcat agtacgcacg ggaatactca tccccagcac
    19081 gccataccag tagttattac tgtctaccgg aacactgacg gtaacctgcg gttcagtatt
    19141 gctggcgtgt tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg
    19201 cgaatggccg ataaaccagg gttgggtgac atagccgtaa taacgcgttg gtacattgcg
    19261 cgtaaacaag gtcggctgcg tcgaaacgta aaatccagca cgcgagacat acatcgcctg
    19321 ttcaaccatc gacgaggagt tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc
    19381 ggtaatttca ttgtcgaggc tttcattttc gcgagacagg aaattcccct cgctgactaa
    19441 tgcatccgat acgccattga ctggcagggt gcgccgtcgg ttgagttcga tttgccaggc
    19501 gtgctcgtcg cgatgctgtt cgaactcggt tacggcatta cgcagagagg tgaaatccag
    19561 tggcgccacg agagcttcgc gcatgccatt acgcaaaaag atcagtttgt cgacgttata
    19621 ctgcaaatgc ttatcgagcg cgttggctac gttttccaga tgattacgct agctggagat
    19681 ataggcatct tccagcacca ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac
    19741 cacgataaag cagagattaa cgacatgacc gggccccagg cggcgtgcga gttttttcaa
    19801 ccagctctgg ttttccattt ttgtctcgtg ctgcaccctg acgactccca acctgctttt
    19861 ttatgattct ggcataaccc ggcaatagcg taccgcaaaa cagcgataat tttataatgg
    19921 tggcatgata agacgcgact atcattcatt gcaggcattg ggacaccagg ccggatgtga
    19981 tccggcctgg tgttaatgat accctttcaa acttcttccg cagaacgact cccctccccc
    20041 ggaacaagat cggtttcatg aaaagcttcc cgcttgaccc cgtaaccgtc ataccaacga
    20101 cactcaacca taccgctgga gtatccagtg acaatcatcc gcgggccgcc ctctttaacc
    20161 gtaacttcct cactaaccat aaagctcata ctcgcctcct tttttcgagt gaaacttgtt
    20221 caccttagtt gaagatggcg aattttgcat aatagccagt gcgagtatta atgtgcctga
    20281 ccgcgcattt tcgctactgc tttcactccc agcaccacga tgccgccgat gataaatcca
    20341 agaatcagat ttaaaacagt cggtaatatc atcgccacca ctgcactttg ctgcccggcg
    20401 atatgttcaa tagcgtgatg cagcggcgca ataccatgta ccacaatccc gccgccgacg
    20461 aggaacatcg ccagcgtgcc gacaatcgat aatgctttca tcagccaagg cgcgataatc
    20521 aataaccctt tacctaatgc ctgcatcagc gcgctagatt tttccgccag ccaataccct
    20581 aggtcatcaa tcttaacgat aacccctacc agaccgtaga cgcccacggt cactaccagc
    20641 gcgatgcctg aaagcaccag cacctgatta agcaacggcg cctctgccac aatccccagc
    20701 gtgatggcga cgatttccgc agacaaaata aaatcggtac gaatcgcccc tttaattttg
    20761 tccttttcaa acttcagcgg atcctgcgcc gccagcttct ccagacgctg ctggctctgc
    20821 gctggatctt ctttatgttt acgcgcctcc agcatatgca gcactttctc taccccttca
    20881 aagcagagaa acgcgccacc aatcatcaac agtggcgtaa tcgcccacgg gataaacgca
    20941 ctgatgatca gcgccagcgg caccagaatc actttattga tcagcgatcc tttcgccacg
    21001 ccccagacca cgggaagttc ccggttggcc cgcacgcctg aaacttgttg cgcattgagc
    21061 gataaatcat cccctaatac accggcggtt ttcttcgccg ccagtttgcc catcacagag
    21121 atatcgtcca gcagtgtggc gatatcatcg agcaacgtca gtaaactact cccggccaaa
    21181 agcattccct ctcattttta ttgttgaagg gtgtaagtat gaagcaaaaa cggcagctct
    21241 gatacttgct tcacaggtca acatttattt aacaatcaag tgcaattata tctctcgcca
    21301 gcagaatgat tttcacgttt actatgaacc acctttttat ttccgccgtg agggattatg
    21361 cgtttccgcc agttgttacc gctttttggc gcgctgtttg cgttgtatat catttggggc
    21421 tcaacctatt ttgtcattcg gattggcgtg ggaagctggc ctccgttaat gatggcgggc
    21481 gttcgattcc aggcagccgg tattttattg ctggcatttt tgctactgcg cggacacaaa
    21541 cttcccccgc tacgtccgct gctcaatgcc gcgctgattg gcctgttatt gctggctgtc
    21601 ggtaatggca tggtgacggt tgccgaacat caaaatgttc cttccggcat cgccgccgta
    21661 gtgattgcaa ccgtgcccct ctttaccctg tgcttcagtc gcctgtttgg cattaaaacg
    21721 cgcaaactgg aatgggtggg tattgccatt gggcttgccg gaatcatcat gctcaatagc
    21781 ggtggaaatt taagcggcaa tccgtggggc gcgattctga ttttaatcgg ctcgattagc
    21841 tgggcgtttg gctcagttta tggctcgcgc attaccttac ctgtagggat gatggcgggt
    21901 gcgattgaga tgctggcggc aggcgtggtg ttaatgatcg catcgatgat tgcgggtgaa
    21961 aaactgacgg cgctcccttc cctgtcaggc ttccttgcgg tcggctatct ggcgctgttt
    22021 ggttcgatta tcgccattaa cgcttatatg tatttaatcc gtaatgtcag tccggctctc
    22081 gccaccagct acgcctacgt taacccggtg gtcgcggtct tgctgggtac gggactgggt
    22141 ggcgaaacgc tgtcgaagat tgaatggctg gcgctcggcg taattgtttt cgcggtggta
    22201 ctggtcacgt tgggaaaata tctcttcccg gccaaacccg ttgtcgcgcc agttattcag
    22261 gatgcatcaa gcgagtaaat gaatcccctg cgtgtcgatc tgcgcgctgg caccttcgcc
    22321 gcagatccac tcttccagac gctcggtaag cgccgcatcc gtcagcttct cgcgcctacg
    22381 taacgcacac tcccagacaa tcagtacgcg ccagccgagc tcctgcaagc gactgatatc
    22441 gcggcgatcg cgttcaacat ttttacctat cttctccagc cagaactcag ttcgagtcgc
    22501 aggcacttta aacagatagc agtgatgatg atgccagaag cagccgtggg taaatatcac
    22561 gcagcgatat tcatcaacga caaaatccgg gcgtccgggc agactggcgt cctgaacgcg
    22621 aaacgcaagg ccctgcccgg ttaacaaact ggcgaggcgc ttctcaatcg ccgtatcacg
    22681 cgtggcaatc gcgcgcatat ttttgctgcg ggtggcctta tcgtgaacgt cgaccatgtt
    22741 gtgcctcttg ctgacgcaac gccaccgcct gtttgatttt tggctcaagc agttttgcca
    22801 ccgcggcaaa gaccggcacg accaccgagt tgccgaactg gcgataggcc tgagtgtccg
    22861 aaaccggaat gcggaatttc gcttctcccg gcgcttcaaa acccattaag cgcgcgcatt
    22921 cacgaggcgt taaccgacgt ggacgatgtt gctgattcag cggatcgtca aagtctttct
    22981 cacccgtggc catatcccaa ccgcgatcga ttaaaatttc cgcgccatct ttgtaataac
    23041 gagcagacag cgtacgggtg acgctttgcg gattgttcgg ataaaccatt ccataaccga
    23101 agccgttacc gcgcgcctga tgttttttcg catatcgata gaggtacttc cacagcaccg
    23161 gcgtcaggat atatttcgcc tcaaccatcg ggtccagcag ctgcgccagc gtcactcgct
    23221 gtgcagggaa acattcgctg atatcacgca gagtaaaatc ggctttcaga ttcagatcgc
    23281 gacgaaaacc caccagcacg atgcgttcac ggtgctgcgg caggaaatgt ttaccatcga
    23341 tgattttcgg atcgtccggc ccgttatctt ctgcatcggc aacgtcatag cccagttcgt
    23401 ccagcgtctg catgatgatg cggaacgttt ttcccttatc gtgactcttc aggtttttga
    23461 cgttttcgag cacaaacatc gccggacgac gcgcgtcgat aatgcgcacc acatcaaaga
    23521 acagcgtgcc ctgggtatcg caggcaaaac cgtgcgcccg cccgagcgag ttctttttcg
    23581 atacgccagc cagcgaaaat ggctgacaag ggaaaccggc cagtaaaaca tcgtgttcag
    23641 gaatgtgttg acgaatatgt tccgccgccg cctcatcact cacgccttct tgatgactga
    23701 gggtgatgtc gcggatatct tcattaaaat gatgcgtcgc cgggtcgcaa taatggttgg
    23761 ctttataagt gcgtaccgca tgtttgttcc attcgctggt aaacacgcac tgtccaccaa
    23821 tcgattcaaa accacgacgg atgccgccaa ttccggcaaa tagatcgata aagcgaaacg
    23881 cataatgcgg atgatgttcc ggtggtttgg gtaacaacgt ttgcagatgg gcaaactctt
    23941 tctcacttaa acggtgccat gccgagtcat tcgccagcgc acgttttaaa attgccgcgc
    24001 tccagtgatt ctcacccaca ccattaagct gcgccaccag cattttaaca tcataaattt
    24061 gcagcagttt ctccagcatt gcctgtgcgg cattcccggt gctgtatgaa tcggttactg
    24121 atatattttc ctgcatagat ttaaccggcc atctgagatg gccgacaggt taacataatt
    24181 actgccgttt agg
//
LOCUS       DAJTRH010000056        24186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTRH010000056 DAJTRH010000000
VERSION     DAJTRH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(25..597)
                     /locus_tag="OO339_002438"
     CDS             complement(25..597)
                     /locus_tag="OO339_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5927036.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="OO339_002439"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="OO339_002439"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5927037.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO339_002440"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO339_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5927038.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO339_002441"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO339_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5927039.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="OO339_002442"
     CDS             complement(3559..5340)
                     /locus_tag="OO339_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5927040.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="OO339_002443"
     CDS             complement(5333..7105)
                     /locus_tag="OO339_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5927041.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO339_002444"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO339_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5927042.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO339_002445"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO339_002445"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5927043.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="OO339_002446"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="OO339_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="OO339_002447"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="OO339_002447"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5927044.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="OO339_002448"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="OO339_002448"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5927045.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="OO339_002449"
     CDS             complement(11661..12032)
                     /locus_tag="OO339_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5927046.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="OO339_002450"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="OO339_002450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5927047.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="OO339_002451"
     CDS             complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="OO339_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5927048.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="OO339_002452"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="OO339_002452"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5927049.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="OO339_002453"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="OO339_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5927050.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="OO339_002454"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="OO339_002454"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5927051.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="OO339_002455"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="OO339_002455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5927052.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="OO339_002456"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="OO339_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5927053.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="OO339_002457"
     CDS             21803..22381
                     /locus_tag="OO339_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5927054.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="OO339_002458"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="OO339_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5927055.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6666 c         5828 g         6025 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaataatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gttata
//
LOCUS       DAJTRH010000057        23909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTRH010000057 DAJTRH010000000
VERSION     DAJTRH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(67..939)
                     /gene="rluF"
                     /locus_tag="OO339_002459"
     CDS             complement(67..939)
                     /gene="rluF"
                     /locus_tag="OO339_002459"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5927056.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1287..2234
                     /locus_tag="OO339_002460"
     CDS             1287..2234
                     /locus_tag="OO339_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5927057.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2304..3110
                     /locus_tag="OO339_002461"
     CDS             2304..3110
                     /locus_tag="OO339_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5927058.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3120..3527
                     /locus_tag="OO339_002462"
     CDS             3120..3527
                     /locus_tag="OO339_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5927059.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3527..4021
                     /locus_tag="OO339_002463"
     CDS             3527..4021
                     /locus_tag="OO339_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5927060.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4087..4884
                     /locus_tag="OO339_002464"
     CDS             4087..4884
                     /locus_tag="OO339_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5927061.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            4895..5683
                     /locus_tag="OO339_002465"
     CDS             4895..5683
                     /locus_tag="OO339_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5927062.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5735..6964
                     /gene="sorE"
                     /locus_tag="OO339_002466"
     CDS             5735..6964
                     /gene="sorE"
                     /locus_tag="OO339_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5927063.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7346..8035
                     /gene="pepE"
                     /locus_tag="OO339_002467"
     CDS             7346..8035
                     /gene="pepE"
                     /locus_tag="OO339_002467"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5927064.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8126..9757)
                     /locus_tag="OO339_002468"
     CDS             complement(8126..9757)
                     /locus_tag="OO339_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5927065.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9977..13660)
                     /gene="metH"
                     /locus_tag="OO339_002469"
     CDS             complement(9977..13660)
                     /gene="metH"
                     /locus_tag="OO339_002469"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5927066.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13860..14684
                     /gene="iclR"
                     /locus_tag="OO339_002470"
     CDS             13860..14684
                     /gene="iclR"
                     /locus_tag="OO339_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5927067.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15001..17185
                     /gene="espL4"
                     /locus_tag="OO339_002471"
                     /pseudo
     CDS             15001..17185
                     /gene="espL4"
                     /locus_tag="OO339_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17154..18891)
                     /gene="aceK"
                     /locus_tag="OO339_002472"
                     /pseudo
     CDS             complement(17154..18891)
                     /gene="aceK"
                     /locus_tag="OO339_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19075..20379)
                     /gene="aceA"
                     /locus_tag="OO339_002473"
     CDS             complement(19075..20379)
                     /gene="aceA"
                     /locus_tag="OO339_002473"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5927068.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20409..22010)
                     /gene="aceB"
                     /locus_tag="OO339_002474"
                     /pseudo
     CDS             complement(20409..22010)
                     /gene="aceB"
                     /locus_tag="OO339_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22279..23208)
                     /gene="metA"
                     /locus_tag="OO339_002475"
     CDS             complement(22279..23208)
                     /gene="metA"
                     /locus_tag="OO339_002475"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5927069.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23365..23808
                     /locus_tag="OO339_002476"
     CDS             23365..23808
                     /locus_tag="OO339_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5927070.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5881 a         6104 c         6067 g         5857 t
ORIGIN      
        1 ctccccgaag gggcgagggg actgtccgag ctctttttgg acgttttcat cagtctgagg
       61 cgttaatcag cgtcccttct ttttacgccc cggcgaggta aaacgcttac cgttggacgc
      121 cgggcgaccg cctttttccg ccgttttttc catcttaacg actggacgtt tgatgcccgc
      181 tgttttcggt ttggccttcg ctttaggttt tgcctcggaa gaggaatttt cgatgagctt
      241 aaagaggtcg attaactcat cgtcggttaa atcgcgccac tcccccagcg gaatgccgct
      301 taagctgacg ttcataatgc gcgtacgttc cagctttttc acttcatagc cgaaatgctc
      361 gcacatgcgc cggatctgac ggttcagccc ctgcactagg gtaatgcgga agacaaacgg
      421 cgcttctttt ttgactttgc actttttcgt caccgtcccg aggatcggca cccccgcccc
      481 cataccacga ataaattcat cggtaatcgg tttatcgacc gtcaccagat actctttctc
      541 atgatcgtta cccgcacgga ggatcttatt caccagatcg ccgtgattgg tgaggaaaat
      601 cagcccctgg gagtctttat ccaggcggcc aatcgggaac acgcgtttgc tgtggttaac
      661 gaaatcgaca atgttatcgc gctcgccatc ttcggtggtg ctgacaatac cgacgggctt
      721 gttcagggcg ataagtacca aatcttcggc ttcccgaggc tcaatcaact gaccgtttac
      781 tttcacaatg tcgccgggtt tcacctgatc gccaatggtg gctcgcttgc cattaaggaa
      841 cacattgcct tgctcgatat agcgatccgc ttcgcggcgt gagcaaattc cgctttcgct
      901 gatgtattta tttaaacgga ctgatgagtc gggcagcata aattctcctg taaaacgcgg
      961 aatataccgt acctcttagc ggggaaaaac agggttatgc ggcaacgtcg aaattaaatg
     1021 tgatctgcca cgccttttat cacaaaagtg tctccggaag tacgatgcaa atttgcctgg
     1081 caaaataagg ataatcaaca tattacgaat cgctgcacac ctgtgctgat gactactacg
     1141 cagagaaaca aatgtgcaag agaaagcgct atttctcccc atgcaaagag tgaaaaatcg
     1201 ctttgaaagg ccctcatgat ggggttatat cagatcaagc ggtgagaaag cgtatttgca
     1261 caaatgtgca gaaccaggag gcggcaatgg aaaacagtga cgatatccgt ttgattgtga
     1321 agattgccca actctattac gaacaggata tgacgcaggc gcaaatcgcg cgcgaactgg
     1381 gtatttaccg caccaccatc agccgcttgc ttaaacgagg ccgcgatcag ggaatggtca
     1441 ccatcgccat caactatgac tacaacgaaa atctctggct ggagcagcaa ctgaagcaaa
     1501 agtttggcct gaaagacgtt gtggtggtgt cgggaaatga tgaggatgaa gagactcaac
     1561 tggcgatgat ggggttacac ggcgcgcaac tgctggatcg cttgctggag cctggcgata
     1621 ttgtcggttt ttcctggggt cgcgcggtga gcgcactggt tgaaaacttg ccgcaggcgg
     1681 ggcaatcgcg gcagttaatc tgcgtaccaa ttattggcgg accgtccggt aaactcgaaa
     1741 gccgctatca tgtaaacaca ttaacctaca gcgcggcagc gaagctgaaa ggggaatcgc
     1801 atctcgcgga ttttccggct ctgctggata acccattaat tcgtaatggg atcatgcagt
     1861 ctcagcactt taaaaccatc tctgcctact gggataatct ggatatcgcc ctggtgggaa
     1921 ttggctcacc ggccattcgc gacggcgcta actggcatgc gttttatggt ggtgaagaga
     1981 gtgacgacct gaatgcccgc caggttgctg gcgatatttg ctcgcgcttt tttgatattc
     2041 acggcgcaat ggttgaaacg aatatgagcg aaaaaacgct ctctatcgaa atgaataaat
     2101 taaagcaggc gcgatattcc attggcattg ccatgagtga agaaaaatac agcggaatta
     2161 ttggtgcact gcgtggaaaa tatattaatt gtctggtaac gaatagcagc acagctgaac
     2221 tattactgaa ataaaaatct tcccgatgtt taccgtaata aaaattacgg cgggtcccta
     2281 ttatctaaaa tcaggagtct gttatgcaaa cgtggttaaa tttgcaggat aaaatcatta
     2341 ttgtcaccgg cggcgcatcc ggtattggtc tggcgattgt agaggaatta ttagcacaag
     2401 gcgcgaatgt acagatggtc gatattcacg gtggcgatgg tcaatatgaa ggtcataaag
     2461 gctatcagtt ttggccgacc gatatttcta gcacaaaaga ggtaaatcat acggtagcgg
     2521 aaattatcca gcgttttggt cgcatcgacg gtctggtcaa taacgccggg gtcaatttcc
     2581 cgcgtctgct ggtcgatgag aaagcgcctg ccgggcagta tgaactcaac gaagctgcat
     2641 tcgaaaaaat ggtcaatatc aaccagaaag gtgtttttct gatgtcgcag gcggtggcgc
     2701 gacagatggt caaacaacat gatggcgtga ttgtgaatgt ttcctcagaa agtgggctgg
     2761 aaggctcaga aggacaaagc tgttacgccg ctaccaaagc cgcgctcaat agcttcacgc
     2821 gctcctggtc gaaagagctg ggtaaacacg gtatccgtgt ggtcggtatc gcgcccggga
     2881 ttctggaaaa aaccgggcta cgcacgccgg aatatgaaga ggcgctggcg tggacgcgca
     2941 atatcaccgt cgagcagctg cgtgaaggct ataccaaaaa cgccattcct attgggcgcg
     3001 ccggaagatt agcagaagta gctgattttg tttgttatct gttgtctgaa cgcgccagct
     3061 acatcaccgg agtaaccact aacattgcgg gcggcaaaac gcgcggctaa ggaggcttta
     3121 tggtcaacgc cattttttgt gcccacggca aattagcttg cgccatgctg gaatcggttc
     3181 agatggttta cggcaacgcc aatgttgaag ccgttgcatt tgtgcccggt gaaaacgccg
     3241 gagacattgc ggtaaagctg gaaaagttag taagcattca caatcaagat gagtggctga
     3301 ttgccgtgga tttacagtgc ggcagcccgt ggaatgcggc agcaatgctt gcgatgcgca
     3361 atcctcgttt gcgggtaatc agcggccttt ctctgccgct ggcgctcgaa ctggttgata
     3421 accaggacag catgaatgtt gatgaactgt gtgaacacct gacgcaaatc gcaaagcaaa
     3481 cctgcgttgt ctggaaacaa ctggcaacga cagaggagga tttctgatga acattacgct
     3541 cgcccgcatt gatgaccgtc tgatccacgg ccaggtcact accgtctggt cgaaagtggc
     3601 taacgcccag cgcattatta tctgcaatga cgaagtttat aacgatgaag tgcgccggac
     3661 attattgcgt caggctgcgc caccgggaat gaaggttaac gtggtcaata ttgaaaaagc
     3721 cgtcgcggtt tatcacaacc cgcaatatca ggacgaaacc gttttttatc ttttcactcg
     3781 cccacaggat gccttagcga tggttcgcca gggggtaaaa ataggcaccc tgaatattgg
     3841 cggcatggcc tggcggcccg gtaaaaaaca gttaaccaaa gcggtttcat tagatgatga
     3901 tgatattaac gcatttcatg agttaaataa tcttggtgtc atactggatt tacgcgtcgt
     3961 agcgtcagat ccctcaatca atattatcga caaaataaac gaacagttaa tcgcaaatta
     4021 aataatagcg cctgtgtaca tatgctttaa caggcaggga tgacgacact taaaaggtga
     4081 caaatcatgg aaataagtac cctacaaatc attgctatat ttcttttttc ctgtattgcc
     4141 ggaatgggca gcgtgctgga tgaatttcag actcatcgcc cgttaattgc ctgtacggtg
     4201 attggtttaa ttctcggtga tttaaaaacc ggaataatac tcggcggtac gctggaattg
     4261 atagctctct gctggatgaa cgtcggcgcg gcgcaatctc cggattctgc actcgccagc
     4321 ataatctccg ccattctggt tatcgttggt cagcaaagca tcgccaccgg aatcgccatc
     4381 gcgttgcctg tggctgcggc aggccaggtg ctgacggtgt ttgcccgtac catcaccgta
     4441 gtgttccagc acgcggcgga taaagcagca gaagaggcgc ggtttcgcac tctcgatatt
     4501 ctgcatgtct ccgcgcttgg cgtgcaggcg ctgcgcgtcg ccattccggc actgattgtc
     4561 tcactgttcg tcagcgccga tatggtcagc aatatgttaa acgccattcc cgaatttgtg
     4621 acccgaggac tacagattgc tggcggtttt atcgtggtgg tcggttacgc catggtgcta
     4681 cgtatgatgg gcgtgaaata tttgatgcct ttctttttcc tcggtttcct cgcaggtggc
     4741 tacctcgatc tcagcctgct ggcgttcggt ggcgtcggcg tgatcatggc cctggtctac
     4801 atccagctaa atccacagtg gtgtaaagct gaacctcaac tccaggccac cgcctccacc
     4861 gcccttgacc aacttgacga ttaacggagc catcatggaa cagagaaaaa ttacacgcag
     4921 cgatctggtg agcatgtttc tgcgctccaa cctgcaacag gcgtccttta actttgaacg
     4981 tattcacggg ctgggctttt gctacgacat gatccccgcc atcaagcgac tttacccatt
     5041 aaaagaggat caggttgcgg cgctcaggct acacctggtg ttcttcaata ccacgccagc
     5101 cgtatgtggc ccggtcatcg gcgcggaaat tgatgacggt accatcaacg gcatcaaagt
     5161 cggtctgatg ggaccattgg caggagttgg cgatccactg gtctggggaa cgctgcgccc
     5221 gattaccgcc gcgctcggcg catctctggc actttcgggc aacattctcg gcccgctgct
     5281 gttcttcttt attttcaacg cggtgcgtct ggcgatgaag tggtatggcc tacagctcgg
     5341 ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg aatgtgctgc aaaaactcac
     5401 cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg ctggtgacca aatggacgtc
     5461 aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc gatggctcca ccgtcaccat
     5521 gaccgtgcag aacattctcg accaactttg ccctggtttg ctggcgctcg gtctgacgct
     5581 actaatggtt cgtctgctca acaaaaaaat taacccggta tggctgattt tcgccctgtt
     5641 tggcttaggg attatcggca atgctctggg cttcctgtcc tgattcttcg ccccggcacg
     5701 actgccgagg ccatcgctca acatgaggtg gtttatgaaa acaacagctc tgcgtcttta
     5761 tggtaaacgt gatttacgcc tggaaacctt tgaccttcct gaaatgcagg aggatgaaat
     5821 cctcgcaacg gtggtcactg acagcctgtg cctctcttcc tggaaagagg ccaatctggg
     5881 tgaaaaccat aaaaaagtac ccgacgatgt gacgaccaac ccaatcatca tcggccacga
     5941 attttgcggc gatattctgg ccgtgggtaa aaagtggcag cacaaattcc agccgggtca
     6001 gcgttatgtg attcaggcca acctgcaact ccccgaccgc ccggattgcc ccggctactc
     6061 cttcccgtgg gtagacggcg aagcgacgca tgtggttatt cccaacgagg tcatggaaca
     6121 atattgcctg ctggcatacg acggcgaaac ctattttgaa ggctcactgg ttgaaccgct
     6181 ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt caggaaggta gttataacca
     6241 cacgatgggg attcgcccgc aagggcacac gctgatcctc ggcggcaccg gaccaatggg
     6301 actgttggcg attgattatg cgctgcatgg accagttaac ccgtcgctgc tcgtcattac
     6361 cgataccgac aacgacaaat tgagttatgc gcgcaagcac tatccatcag aaccgcaaac
     6421 actgattcat tatctcaatg ccaccgatgc agcatttgat acgttaatgg cgctgagtgg
     6481 cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat gaaggactgg tgactctcgc
     6541 ctcttccttg ctggcgacag atggttgcct gaatttcttc gccggaccgc aggataaaca
     6601 tttcagcgcg ccaattaatt tctacgatgt gcattatgca tttacccact acgtgggcac
     6661 gtcaggcggc aataccgacg acatgcgcgc ggcggtcaaa ctgatcgaag agaaaaaagt
     6721 gcaggccgca aaagtggtaa cacatattct tgggctgaat gccgcgggcg aaaccacgct
     6781 tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat accgggaaat acctgccgct
     6841 gacgtcactc acgcagattc aggatcaagc actggcggcg attctggcgc gtcatcaggg
     6901 gatctggtcc ggtgaggtgg agcaatacct gctcgcccat gcagaggcaa tttcccatga
     6961 ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg ccccagcaac ttcgcgaccg
     7021 gatgtgcggc tgattgctga agcagcacaa tttgcacgag aaggataaaa aatatcagcg
     7081 ccttaagggg cgctcaaatt ggtggcaaag tacacattgt ttatgccgga tgcggaatga
     7141 agccttattc ggtctacata attttgaaaa tccaatatat tgcagttatc tggtaggcca
     7201 agtaagcgta gcgcatcagg caatgctgtg tttgtcatca gtctcagcgc ccggtgcaat
     7261 acgtgacatc gtgagccatg tcagtaagtg gttaaattcg cagtggtagc atgccttccg
     7321 tgatttctta ctaaccggag aagtaatgga actgctttta ttgagtaact cgacgctgcc
     7381 gggtaaagcc tggctggaac atgcactgcc gctaattgct gaacagttgc agggtcgccg
     7441 ctcagcggtg tttatccctt tcgctggcgt aacgcagacc tgggatgatt acacagagaa
     7501 aacggctgcg gttctcgctc cgctgggtgt ttctgtcacc ggtattcata gcgttgtcga
     7561 tcccgttgcc gcgattgaaa atgcggaaat tgtgattgtc ggcggcggta atactttcca
     7621 gttgctgaaa cagtgccgcg agcgcgggct gctggctcca attactgacg tggttaaacg
     7681 tggcgcgctg tatattggct ggagcgcagg cgctaacctt gcttgcccaa ccattcgtac
     7741 caccaacgat atgccgattg tcgatccgca aggtttcgat gcgctaaatc tgttcccgtt
     7801 gcaaatcaac ccgcacttca ccaacgcgct gccggaaggc cataaaggtg aaacccgtga
     7861 gcagcgtatt cgcgaactgc tggtcgtcgc gccagaactg acgattattg gtctgccgga
     7921 aggtaactgg atcacagtga gtaaaggtca cgctacgctg ggtggcccga acaccactta
     7981 tgtgtttaag gctggtgaag aagcggttcc gctggaagct ggtcaccgtt tttaagtgaa
     8041 ttgccggatg catgatccgg catcttgttc atagtgcctg atgcgacgct tgacgcgtct
     8101 tatcaggcct acaggattca caatattaat actcgtcccg tccttcatct tcatccggct
     8161 gttccagcac actgtaagcc accgaacaaa acagcgagtt caggcgctgc atatctccca
     8221 gtaagcctaa atgtagcgaa ctggtttcaa tgctctgcac gttttgctga tgcaggcgat
     8281 cgacgtgggc gtgggaatag cggcgattaa gaatgcgaaa acgatgtttg ctgcgacgca
     8341 aacgacgagc gctggtgaca tcgccagaga agaacaccga cattgccagc tttaaattac
     8401 tgagcaattg ctcatagagc gcatccagtt ccttcaaccc atcaagcgaa aatgcccgcc
     8461 gtgctgccag cgatttatcg gcaatttcgc tgcccatgcg ctcgacgata tcggaggcct
     8521 gttcaaggtt gagcgacatt tcgatgatct ccgcccagcg gcgcgactct tcctccgcca
     8581 actcttcttt tggcatccgc gccagataca gcttaatggc ggtgtagaga acgttgatat
     8641 catctgccag cttacgcagc tctttctcct gccgtggctc gccgtgcatc actttattca
     8701 gcccttccat catctgttcc atggcgtcgc caatgcgcag ggtttcgcgc gcggcgttcg
     8761 ccagagcaag cgtgggcgtg tccagcgcgc tgacatccag atgtttaggc cgtagctggg
     8821 tatccagttc cggttcatcg cgaataatcg ttttgcaaaa ccgtgccatc gggtcaacaa
     8881 atggcagcat gaccaggcaa cgtacaaggt tgtagaagac gtggaaatag atcaccagtt
     8941 ccgcttttgg caatgacagc ttccccattg tctctgccag caaatggaca aacggcagga
     9001 taatcaggct acccaccagc ttaaacagca gactacccag cgcgacacgg cgggctgcgg
     9061 cattggcggc actgttgttg agcatcgcca gcaggccgga accgaggtta gctccaatca
     9121 ccagacagag cgccacgggg aaggagataa tgcctgcagc ggtcagagtc gcggtcagca
     9181 gtacagcagc aaggctggag tagctgataa tggcgaacat cgcgccaatc agcgcatcca
     9241 gcagaatatc gccggtcagc gaggcaaaga tcacctgaac gccgtttgcc tgggtgatcg
     9301 gcgttacggc ctgcacaatc aactccagcg ccagcaaaat cagcccaaga ccaataccga
     9361 cgcggcccag ttgcccggcg cgtgactgtt tgcgtccgag gaaaaaaatc acgccgataa
     9421 aaataagtaa cggtgacagc caggataagt cgaaggtgag aatacgcgcc attagcgccg
     9481 tcccgacatc tgcccccagc acaatgacca gagccggtgc gagggctacc agatcctgag
     9541 cgacgaacga ggtcaccagc atggtggtgg cattactgct ctgtaccagt gcggtaacgc
     9601 cgatccccgc gcaaaaggcg agcggctttt tttcgacgct ccggctaagg acagtacgca
     9661 aacgcgcgcc gaagacgcgc attacgccgg ttcgaacaat atgagtcccc cagaccagca
     9721 gggcgacggc agaaagcagg tgaagcagcg ttaacacgag ttcagattct ccttatcgtt
     9781 atatgttcct gaagcaattg aatgaccaaa ggaccggaaa cacgctgact ggcatcttta
     9841 ctgtgccttt tgacattcgt ttttccggct aagattattc cagcctgttt ccagtataag
     9901 tgtttcatat taataaagag acagggcact cattgagcgc cctgtttgtt gtaaggaaaa
     9961 gtgacagatt tgtgaatcag tccgcgtcat accccagatt cggtgccagc cagcgctcaa
    10021 catcggagac gctcatacct ttacggcggg catggtcttc aacctgatcg cgctgaattt
    10081 gcgccaccgc gtagtacttg ctgtccggat gactgaaata ccaacccgaa accgatgcac
    10141 caggccacat cgcgaaagat tctgtgagtt tcatgccagt atgtttttcc acttccagca
    10201 gctcccagat ggtggctttt tccgtatgtt ccgggcaggc cggatagccc ggtgccggac
    10261 ggatgccctg gtagttttcg cggatcagtt cttcgttgct gaggttctcg ttcggcgcat
    10321 agccccagta gactttacgc acacgctcat ggagatactc cgcaaaggct tcggctaaac
    10381 ggtcggcaag cgctttcacc atgattttgt tgtaatcatc gtgctgcgct tcaaaggcat
    10441 cagccagtgc gtcctcttcc agcccgccag tcacggcaaa tgcgccgatg taatctgctt
    10501 taccagaaag cttcggcgca acgaagtcag cgagacagta gttagcgaat cccgtttttt
    10561 cggtctgttg acgcagatgg tggctgacgt tgatcacatg ggtgcgcgtt tcgtcacggt
    10621 agatttcaat gtcatcgccc acacggtttg ccgggaacag gcccaccacg ccacgcggat
    10681 tcagcgtttt ctcggcgctt aatttatcca gcatgtcgtt ggcgtcttta aacagccgct
    10741 gcgcctcaac gcccaccact tcatcttcca gaatgcgagg atacttcccg gccagcgacc
    10801 aggtcataaa gaacggtgtc cagtcgatgt aattacgcag cgtttcgatg ctggcttcca
    10861 cttcctgcac gccgagacgg tgcgccaccg gcggcgtgta agcctgccag tcaaatgcga
    10921 aatcgttatc gcgcgccgct tccagcgtga ccggtggtgt gcgtggtttc ttgcgcccgt
    10981 gctgaatacg cacggtttcg tactccttgc aggtacgagc gacaaaatca tcacgctgag
    11041 tatcggaaag cagcgccgct accacaccaa cggtgcgcga ggcattctgc acatacaccg
    11101 tagggccgct gtagttctgc tcgattttca ccgccgtgtg cgcttttgag gtggtcgcgc
    11161 cgccaatcag taacggaatg gcgaagccct gacgctccat ctctttcgcc acgttaacca
    11221 tctcgtccag cgacggcgtg ataagccccg aaaggccaat caaatcagca ttcacttctt
    11281 tggcgatacg gagaattttt tccgcaggca ccataacgcc gagatcgaca atttcgtagt
    11341 tgttacattg cagcaccaca ccaacgatat ttttaccgat gtcgtggaca tcgcctttca
    11401 cggtggcgat caccatcttg ccgttggttt taccctgctc tttgctggct tcaataaacg
    11461 gttcgagata cgccactgcc tgtttcatga cgcgcgccga tttgaccacc tgtggcagga
    11521 acattttgcc ttcgccaaac agatcgccga ccacgttcat gccgtccatc aacgggcctt
    11581 caatcacttc aatcgggcgc gtagcctgct ggcgggcttc ttcagtatcc tgctcgataa
    11641 actcggttat gcctttgacc agcgagtatt ccagacgttt attcacgtcc cacgagcgcc
    11701 actccgcttg ctgggcgtta gcagtgtcgt ccgctttact gccgcgatat ttctcggcaa
    11761 gctccagtaa acgctcagtg ccatcgtcac gacgattaag aatcacatct tccaccgcgt
    11821 cgcgcagttc agcgggtagg tcgtcgtaaa tcgccagttg cccggcgttg acgatcccca
    11881 tatccatgcc attgcgaata gcgtagtaga ggaacactgc gtgaatggct tcgcgcaccg
    11941 gatcgttgcc acggaatgag aaagaaacgt tagatacgcc gccggaaatc agcgcgtgcg
    12001 gcagttcgcg tttgatgtct tcacacgcgc cgataaagtc ctgcgcgtag ttgttgtgct
    12061 cttcaatacc ggtggcgacc gcgaagatgt tcgggtcgaa gatgatatct tctggcggga
    12121 agccaacctc ttcggtgagg attttgtacg cccgacggca aatctcgatt ttccgtgcgc
    12181 gagtatcggc ctgtccctgt tcgtcaaagg ccatcaccac cactgccgca ccgtagcgac
    12241 gcaacaattt cgcgtgatgg ataaaggcgt cgacgccctc tttcatcgag atagagttaa
    12301 caatgccttt gccctggata cacttcagac ctttttcaat gacatcccat tttgaggagt
    12361 cgatcataat cggcacgcga gcgatatccg gttcaccggc aatcagattg agaaaacgca
    12421 ccatcgccgc ttcggcatcg agcatccctt catccatgtt gatatcgata atctgcgcgc
    12481 cgttttccac ctgctgacgc gccacatcca gcgcctcgct gtatttctct tctttgatca
    12541 ggcgcttgaa cttagcagag ccggtgacgt tggtgcgttc acccacgttc acaaacaggc
    12601 tatcgtcgcc aatgttcagc ggctccaggc cagacaaacg gcaggctacg ggaatttccg
    12661 gcagtttgcg cggcgctaat ccttctactg cacgactcat cgctgcaata tgttgcggcg
    12721 tggtcccgca gcaaccaccg acgatattga gaaagcccgc ttgcgcccat tcacgtatct
    12781 gttttgccat cgtgtcggcg tcgagatcgt attcaccaaa ggcgttaggc agcccggcgt
    12841 tcgggtgggc ggtgacgtag cattccgcaa tccgtgatag ttcctgcacg tactggcgca
    12901 gttcatcggg ccccagcgca cagttcaggc caaaggtcag cgcttcggcg tggcgcaatg
    12961 agttgtaaaa tgcttcggtg gtctgcccgg agagcgtgcg cccggaggcg tcggtgatgg
    13021 tgccggagat cataatcggc agctcaacgc ccagcgcttc aaactccgtt ttcaccgcaa
    13081 agaccgccgc tttggcgtta agggtgtcga aaacggtttc aatcaggatc agatccgcac
    13141 caccttccac cagcgctttg gtggattcgc gataggccgc cgccagctgg tcaaaagtga
    13201 tgttgcggaa tgccggatcg ttgacgtccg gagaaataga cgccgtgcgg ttggttgggc
    13261 caagaacacc ggcaacgtag cgcggtttct ccggcgtgcg cgcggtccac tcgtcagcac
    13321 aggcgcgcgc cagtttcgcc gccgcaaagt tgatttccgc cgacagggat tccatctggt
    13381 aatccgccat cgcaatggtc gtggagttga aggtgttggt ttcgatgata tccgcgcccg
    13441 cttcaaagta ggcgttgtgg atagcggcga tcacttccgg tttactgagt accagcaggt
    13501 cgttgttgcc tttgaggtcg catggccagt cggcaaagcg ttcaccacga aaatcggctt
    13561 cgttcagtcg atagctctgg atcatggtgc ccataccgcc gtccaacacc agaatacgtt
    13621 catttaactg cgcacgcagt tgttccactt tgctgctcac acttgctccc gacacgctta
    13681 acccagattt aataaaaatt caacaaacca tactggaata aacgcatctg tggtaaaagc
    13741 gaccaccacg caacatgaga tttgttcaac attaactcat cggatcagtt cagcaactat
    13801 tgcattagct aacaataaaa atgaaaataa tttccacgat acagaaaaag gaggctgtca
    13861 tggtcgcacc cattcccgcg aaacgcggca gaaaacccgc cgttgccacc gcaccagcga
    13921 ctggacaggt tcagtcttta acgcgtggcc tgaaattact ggagtggatt gccgaatcca
    13981 atggcagcgt ggcactcaca gagctggcgc aacaagccgg gttacccaat tccacgaccc
    14041 accgcctgct aaccacgatg caacagcagg gtttcgtgcg tcaggtcggc gaactgggac
    14101 actgggcaat cggcgcacat gcctttatgg tcggcagcag ctttctccag agccgtaatt
    14161 tgttagcaat tgttcaccct atcctgcgca atctaatgga agagtctggc gaaacggtca
    14221 atatggcggt gcttgatcaa agcgatcacg aagcgattat tatcgaccag gtacagtgta
    14281 cccacctgat gcgaatgtcc gcgcctatcg gcggtaaatt gccgatgcac gcttccggtg
    14341 cgggtaaagc ctttttagcc caactgagcg aagaacaggt gacgaagctg ctgcaccgca
    14401 aagggttaca tgcctatacc cacgcaactc tggtgtctcc tgtgcattta aaagaagatc
    14461 tcgcccaaac gcgcaaacgg ggttattcat ttgacgatga ggaacatgcg ctggggctac
    14521 gttgccttgc agcgtgtatt ttcgatgagc accgcgaacc gtttgccgca atttctattt
    14581 ccggaccgat ttcacgtatt accgatgatc gcgtgaccga gtttggcgcg atggtgatta
    14641 aagcggcgaa ggaagtgacg ctggcgtacg gtggaatgcg ctgacttttt ctggcgggca
    14701 gaggcaatat tctgcccatc atacctgagt ggcaatagaa taagggtgtc tgttaatcgc
    14761 attgacgcca aaataactta atgtcataca cttcacacac ttttattaac tcagcattat
    14821 ttttaaacat caaaccactt aattattaac aaaatcgcaa gatctcattc caccgaaact
    14881 tttacggctg cattatgaga ttgagcgatt tatgatattt ttttagttgt aatcatatta
    14941 aattttttca ctgttctgat agttaaaatt caagacatca ataaacaatg agatatttaa
    15001 atgattactc gtattcctcg tagttctttc tctgcaaata ttaataatac agcccagaca
    15061 aatgaacacc aaaccctgag tgaattgttt tataaagaac tcgaggataa attttctggc
    15121 aaggagctgg cgacgcctct attaaaaagc ttctcagaga actgtagaca aaatgttcgt
    15181 catatcttta gcaacaagga ttttgtcatt aaattttcca cgtccgtctt acaagctgat
    15241 aagaaagaaa ttacgatgat taataaaaac gaaaacacga cactcactca aaccattgct
    15301 acaatatttg aagaatacct aatggaaatt ttacctcaac gctcagacac tcttgataaa
    15361 caagaattaa accaaaaatc agatagaaaa gaaaaagaat tcccaagaat taaacttaat
    15421 ggtcaatgtt atttcccggg gcgccccaaa accgtatagt ataccgacac attgctgcac
    15481 aatatattaa tgatatttat cagaatgttg attacaaacc ccatcaagat gattactctt
    15541 cagctgaaaa atttctcact cacttcaaca aaaaatgcaa aaaccagact ttggcgttga
    15601 tttccagccg tcctgagggg cgttgcgttg ctgcctgcgg tgatttcggg ctagttatga
    15661 aagcatattt tgacaagatg gaatcaaatg gcatcagtgt tatggcagcc atattactgg
    15721 tggataacca tgctttgacg gtccggctaa gaataaagaa cacaactgaa ggatgtaccc
    15781 attacgtggt ttcggtttat gatcctaatg taactaacga taaaataaga attatgagcg
    15841 aaagcaaaga ggatattaaa cactattctc tgatggattt tatgaatgta gattatagcc
    15901 tcctgaaatg gtcaaatgat catgttatta accaatctgt tgcaataatt ccagcacttc
    15961 cgaaagaaca gctattgatg ttaaaaggaa ctgtggatga aataacccct ccattatcac
    16021 cagcaacgat gaatttgcta atggcaattg gtcagaatca ccaacttacg caactgatga
    16081 ttcagctcca gaaaatgcca gaactacata gaacagaaat gttgactgcc tataatagta
    16141 ttaacctccc cggtttatat ttggctataa attatggcaa tgcggatatc gttgagacta
    16201 ttttcaattc attgtcagaa acaggatatg aagggttact ctcgaaaaaa aatctcatgc
    16261 atattctgga ggcaaaagat aaaaatggtt tttctggatt atttttagcg atatcacgta
    16321 aggataaaaa tgttgtaacc tcgattctga acgtcttacc taaactggcc gcaacacatc
    16381 atttagataa tgaacaggtg tataaattcc tgagtgccaa aaatagaacg tccagccatg
    16441 ttttatacca tgttatggcg aatggtgatg ccgacatgct gaaaattttt ttggtcgcgt
    16501 tacctttgtt aattcgcaca tgtcatttga ctaaagaaca ggtactcgat ctcctgaagg
    16561 caaaggattt ttatggttgc ccaagactat acctggcgat gcaaaatgga catagcgata
    16621 tcgtgaaagt tattctcgaa gcattgccct gtctggccca ggaaattaac atttcagctt
    16681 ccgatattgt agatcttctg accgctaaaa gtcttgcgcg cgacacgggt ttgtttatgg
    16741 ccatgcagcg cggacatatg aacgttatta atactatttt taacgcatta cccactcttt
    16801 ttaatacgtt taaattcgat aaaaaaatat gaagcccctc ctcctggcaa ataattctaa
    16861 tgaatatccc ggtttgtttt cagcgataca gcataaacaa caaaacgttg tagagacggt
    16921 ttatcttgct ttatctgacc atgcacgcct gtttggattt accgctgaag atattatgga
    16981 tttttggcaa cacaaagcgc cacaaaaata ctctgccttt gagttggctt ttgaatttgg
    17041 tcaccgggtt attgctgaat taatccttaa tacattaaat aagatggctg aaagctttgg
    17101 ctttacggat aaccctcgat acattgcgga gaaaaattat atggaagctt tactcaaaaa
    17161 agcatctccc cataccgtac gctaaatctt tgcctgcgcc gatacgcata aacatcttcc
    17221 acatgccctt cacgtatgcg gttttgtagt gcgcgccagt aatcagcgcg gaacaggtcg
    17281 gcgtgcatct cttcaaacag cggaccaata cgcgggtcgg cgcatagcca gtggcgaaac
    17341 tcttccggga aaacatcgcc cggcgagacg ctgtaccacg gttcgctggc aagttcgtct
    17401 tccggatagc gcggcggcgg gatgtcgcgg aaattcactt ccgtcatgta gcaaatttca
    17461 tcgtaatcat aaaaaaccac acgcccgtga cgggtgacac cgaagttttt aaacagcatg
    17521 tcgccaggga aaatgttagc agcggcaagc tggcgaatag cgttaccgta ttcttcaatg
    17581 gcgtcgcgca actgctgacc ttccacttgt tccagccaga tattgagcgg caccatccgc
    17641 cgctcgatta tagagatggc gaattacaat ctgctcacca agatcggtga ttttttccgc
    17701 tgcttcctgg agcagtaatt ccattaatac cggggaaata tgccgcttct ccagcacaaa
    17761 gttttcaaac tcctgggtgt ccgccattcg gcccacacgg tcgtgctctt tcaccagttg
    17821 atagcaggca cgaacgtgag cggcagacat ctctttctgc ggcgcgaact tgtctttgat
    17881 gactttgaat acccgatcaa agcccggcag cgtaaacacc aacatcacca ttccacgaat
    17941 acccggcgct tcaatgaact gctcattaca gccctgtaga taaacgagat attcgcggta
    18001 gctttcggtt ttggcgtgct tctggcagcc gatagccata tacaattcag cagtggtttt
    18061 acctggcaga atttcccgta gccactcgac cagcgctgcg ggcagcggtg cataaaccat
    18121 aaaataagaa cgcgcaaagc caaaaacaat gctcgcttcg gcggtcgtcg tcaggcaggt
    18181 atcaataaat aactcgccgt cgtccgtctg gtggatcggc agtaagaagg gcaacgtgcc
    18241 ggaaggcgtg atcagtttgc ccaccagcca ggcggcttta ttgcggtaaa acagttcgtt
    18301 cgccacctgt aaatgacttt ccgcgaggtt gtctgtcccc agcgtttccg tcagatggcg
    18361 aatgatgtaa tgaatgtcac ggcttttatt ctgccagcgc aggcgcagcg gcaggtcgct
    18421 gataacgcgc atcagtagag attcccagcc gtgatcgggg tgaaagtctt tcgccagcgg
    18481 gcggggaatg gtacgaaagc ggcgctctgg ctgagagcta aagataaaaa gccgctcggg
    18541 agtaagcgag cggtggtcaa ataaccgaca gtacacggag ttaaaaaagc tctccgcaat
    18601 ctcgaagcgc gggtaatccg gcaacagccg cgtgtaatgc tctttaacgc gcagtaaaaa
    18661 tgccgcgtcc gtactttggc cgttagtaat gcagcgcagt tgctccacga ccagaccaac
    18721 gtgatgatcg taaagatgga tacggttttt catcgcctgc tggacagcat gccagtcggc
    18781 ctgttcgaaa cgctgctgcg ccccggaagt cacttcgagg aatcgaccat actgagcgtc
    18841 gaagccttgc aaaatggttt gagcaatcag taattccagg ccacgcggca tttagcgccc
    18901 tcatcaggag cagagaattg ccggatgcgg cgtaaacgcc ttatccagcc tacgttcggc
    18961 aacggttgta ggcatgataa gacgcgcaag cgtcgcatca ggcatcgtgc accgattgcc
    19021 ggatgcggcg taaatgcctt atccggccta cagtcagcaa cgtttgtagg ttgcttagaa
    19081 ctgcgattct tcagtggagc cagtcagcgc ggtgactgaa gacgtgccgc cctgaataat
    19141 agtcgtcact ttatcgaagt aacctgtacc cacttcctgc tggtgagata cgaaggtata
    19201 gccatctttc gcggcggcaa attccggctg ctgcactttc tcaacatagt gcttcatacc
    19261 ttcgccctgc gcataagcgt ttgccaggtc gaacatgtta aaccacatgc tgtggatacc
    19321 tgctagggtg atgaactgga acttgtagcc catatccgac agctgctgct ggaagctggc
    19381 aatagttttg tcgtcgaggt ttttctgcca gttgaacgac ggcgagcagt tataagccag
    19441 cagtttgccc ggatatttcg cgtggatagc ttgtgcaaag cgacgcgcca gttccagatc
    19501 cggcgtggag gtttcacacc agaccaggtc agcatatggc gcatacgcca ggccacggct
    19561 gatcgcttgc tcaatgcccg catgagtacg gaagaagcct tcactggtac gctcgccggt
    19621 aataaattcg ctgtcatacg ggtcgcaatc tgaggtgatc agatccgccg catcagcatc
    19681 ggtacgtgca accagcaggg ttggaacgcc cgtcacgtca gctgccagac gcgccgcgac
    19741 cagtttctga atagcttcct gagttggcac taaaactttg ccgcccatgt gaccgcattt
    19801 cttcactgac gccagctgat cttcgaagtg aactgccgca gcaccggctt caatcatcgc
    19861 tttcatcagt tcaaaggcat tcaggacacc gccaaaaccg gcttccgcat cggcaacgat
    19921 cggcaggaag taatcgacat agcgcggatc gcccggctca atgcccacgg accattggat
    19981 ctgatcggca cgacggaagg tgttgttgat ccgctccacc acagctggca ccgagtttgc
    20041 cggatagagc gactgatccg gatacatgct ggccgccagg ttagcgtccg ccgctacctg
    20101 ccatcccgac agatagactg cttcaatacc cgctttcgcc tgttgcagcg cctgaccgcc
    20161 agtcagtgca ccgaggttgt tgatgtagcc ttttttcgac tcaccgtgca gcagacgcca
    20221 cattttcgct gcgcccagtt gcgccagcgt gcattcagga ttgactgaac cgcgtaattt
    20281 caccacatct tccgcactgt atgggcgagt aatgccttcc caacgcggtt gagtccactc
    20341 tttctgtaat tcttcaattt gttgtgtacg ggttttcatg tgcagatgct ccatagttat
    20401 gtggtggttt acgctaacag gcggtagcct ggcagggtca ggaaatcaat taactcatcg
    20461 gaagtggtga tctgttccat caagcgtgcg gcatcgtcaa aacggccctg ggagaaacgt
    20521 tcttcgccca gttcgctggc aatgactttc atctcttcgc ccagcatctg gcggaacaag
    20581 gctttggtca ccggtttgcc attgctcaac gttttttgat gatggatcca ctgccagatc
    20641 gaggtacggg aaatttcagc cgtcgccgca tcttccatca ggccataaat cggcacgcag
    20701 ccgttgccgg agatccacgc ttcgatgtac tgcacagcca cgcgaatgtt agcgcgcata
    20761 ccttcttcgg tgcgttcacc atcacaaggt gccagcagct gatcggcagt aatcggcgcg
    20821 tcttgttcgc gcaatacttc aagctgattt ttacgggagc cgagaatgtc gttgaatacc
    20881 gccattgccg tgtccgcaag gcctgggtga gcgatccatg tgccatcgtg accgttattg
    20941 gcttccagcg ctttatccgc ttttactttg ttgagcacct ggttattacg ctcttcatct
    21001 ttgctcggaa taaacgcggc catgccgccc atcgcaaaag caccgcgttt atggcaggtt
    21061 ttaatcagca ggcgtgagta agcattcagg aatggtttat ccatcgtcac ggcctgtctg
    21121 tctggcagga cacgatcggg atagtttttc aacgttttga tatagctgaa gatgtaatcc
    21181 cagcgaccgc agttcagacc aacaatatgg tcgcgcagcg cgtgaaggat ttcgtccatc
    21241 tggaacacgg cgggcagcgt ttcaatcagc aacgtcgcct tgatggtgcc gcgcggcaga
    21301 ttaaagcgat cttctgcata gctgaagact tcgctccacc aggccgcttc ctgctaggac
    21361 tgggttttcg gcagatagaa atagggaccg ctgccctttg ccaacagtgc ctgatagttg
    21421 tggaagaaat agagcgcaaa atcaaacagg ctaccgggga ttgcctcacc acgccaggtg
    21481 acatgttttt ccggcaagtg cagaccgcgt acccgacaaa tcaaaaccgc tggattgggc
    21541 ttgagctgat aaattttgcc tgcttcattg gtatagctga tggtaccgtt aaccgcatcg
    21601 cgcaggttaa tttgcccgtc gatcactttg ttccagtctg gtgccagtga atcttcgaaa
    21661 tcggccataa agactttcac attggcgttc agtgcgttga tcaccatctt gcgctctacc
    21721 gggccagtta tctctacgcg gcggtcttct aagtccgcag gaatcccgcg aattttccaa
    21781 tcagcatcgc gaatggaagc cgtttccgaa ataaaatcag gcaacgttcc gttatcaata
    21841 tcttgctgct gctgaatgcg cgctgccaga agtttattgc gttgtggcgt aaaatgcgtc
    21901 accagctcag tcagaaattc taccgcttcg gcagtaagaa tttgcttctc ctgctcgcca
    21961 tacggccttg tgaaagccag ttcatcggtt gttgttgcct gttcagtcat cgtgcagctc
    22021 ctcgtcatgg atccgaaaac ttcccctctg tgaacgaagg aacgttgtgc gcttttcgtt
    22081 cagcacaact aagactactc atttaaaatg caaaatcaaa aacaatttcc attttaatta
    22141 aaaatacttg ataacttatt gataacaata tgaataaaat ttattcactt gctgaggtgc
    22201 gtttcggaaa taaaaaaggc acccgaaggt gcctgaggta aggtgctgaa tcgcttaacg
    22261 atcgactatc acagaagatt aatccagcgt tggattcatg tgccgtagat cgtatggcgt
    22321 gatctgatag acgtaatagt tgagccagtt ggtaaacagc aaattaccgt gactacgcca
    22381 gctcgctcgc ggtttgtttt gcggatcgtt gtgcgggaaa tagttatacg gtacatccgg
    22441 gtctagtccg gcttccacat cgcgtaaata ttcctgcgcc agcgtttgcg catcatattc
    22501 gggatggccc gtcacaaagg caatgcgctt atctttgctg gcaaacagat atgcatcccc
    22561 ttcttccgtc tctgccagaa tttccagatc ggtgtaatca cgaatcaacg ctgccggaaa
    22621 gtcagcatag cgcgaatgcg gtgccaggaa tgaatcatca aagccacgcg tcagaagcgc
    22681 atgaggatgg agaatatgat gctcgtaaac gccagagagt ttgtcggtgc gagtttgctt
    22741 aggaatgccg tagaggatat tgagcgcggc ctgtaccgcc cagcagacaa acagcgtcga
    22801 ggtgacgtga tctttcgacc actccagcac ctgtttgatc tgcggccagt aagcgacatc
    22861 attaaactcc accaggccca gcggcgcacc ggttacaatc agaccgtcaa agttctgttc
    22921 ctgaatatct tcaaagttac agtagaagtt gttcagatgc tctgcgggcg tgttgcgcga
    22981 ttcacgggaa tcgatgcgca acagctgaat atcgacctgc aaaggtgagt ttgaaagcag
    23041 gcgcagaaac tgattttcag tttcaatctt cttcggcatc aggttaagga tcagaacctt
    23101 aagtggacga atttcctgac cagacgcacg agaagttgtc atcacaaaga cgttttcttc
    23161 acgcaagaaa ttgacggcgg gtagctcgtc cggcacacga atcggcataa cctgattacc
    23221 tcactacata cgcttatacg tttagacatc cagatagctg aagataacca gaaaatttgc
    23281 caatgtcgag cctgcatgtt gaaggtgaga aagtttcaag gaaaaggcta cgttagaata
    23341 taagaatgac gaaaaggaga gaaaatggtt attagtattc gccgctcacg gcatgaggaa
    23401 ggggaggaac tcgttgcgat ttggtgtcga tctgtcgatg ccacccacga ttttttatca
    23461 gcagagtatc gggccgagct ggaagagctg gtgcgttcct ttctgccgga agcaccgttg
    23521 tgggtcgcgg ttaatgaacg ggagcagccg gttgggttta tgttgttaag tgggcagcat
    23581 atggatgcgc tgtttatcga tcctgatgtg tgcggctgcg gcgtgggtcg gatgctggtg
    23641 gagcatgcgc tctcgatggc accggaactg acaaccaacg ttaatgagca aaatgagcag
    23701 gcggttgggt tctataagaa ggtgggtttt aaggttacgg gacgctctga ggtggacgat
    23761 ttggggaaac cgtatccgtt gctgaatctg gcgtatgtgg gggaataagc cgcctctgcc
    23821 gtgcctgccc aagctggaga agacgcgcca gcgtcgcacc cggcattttt ttcaccaacg
    23881 caaaaaggcc atcctgacgg atagccttt
//
LOCUS       DAJTRH010000058        22961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTRH010000058 DAJTRH010000000
VERSION     DAJTRH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            89..865
                     /gene="exuR"
                     /locus_tag="OO339_002477"
     CDS             89..865
                     /gene="exuR"
                     /locus_tag="OO339_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5927071.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1210..1872
                     /locus_tag="OO339_002478"
     CDS             1210..1872
                     /locus_tag="OO339_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5927072.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1876..2259
                     /gene="mzrA"
                     /locus_tag="OO339_002479"
     CDS             1876..2259
                     /gene="mzrA"
                     /locus_tag="OO339_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5927073.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2406..2760
                     /locus_tag="OO339_002480"
                     /pseudo
     CDS             2406..2760
                     /locus_tag="OO339_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2798..3103
                     /locus_tag="OO339_002481"
     CDS             2798..3103
                     /locus_tag="OO339_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5927074.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3106..3510
                     /locus_tag="OO339_002482"
     CDS             3106..3510
                     /locus_tag="OO339_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5927075.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3500..3799
                     /locus_tag="OO339_002483"
     CDS             3500..3799
                     /locus_tag="OO339_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5927076.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3985..4281
                     /locus_tag="OO339_002484"
     CDS             3985..4281
                     /locus_tag="OO339_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5927077.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4351..5337
                     /gene="yqjG"
                     /locus_tag="OO339_002485"
     CDS             4351..5337
                     /gene="yqjG"
                     /locus_tag="OO339_002485"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5927078.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5630..5998
                     /locus_tag="OO339_002486"
     CDS             5630..5998
                     /locus_tag="OO339_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5927079.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6205..7101)
                     /gene="yhaJ"
                     /locus_tag="OO339_002487"
     CDS             complement(6205..7101)
                     /gene="yhaJ"
                     /locus_tag="OO339_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5927080.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7206..7907
                     /locus_tag="OO339_002488"
     CDS             7206..7907
                     /locus_tag="OO339_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5927081.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7930..8094
                     /locus_tag="OO339_002489"
     CDS             7930..8094
                     /locus_tag="OO339_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927082.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8306..9616)
                     /locus_tag="OO339_002490"
     CDS             complement(8306..9616)
                     /locus_tag="OO339_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5927083.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9644..10975)
                     /locus_tag="OO339_002491"
     CDS             complement(9644..10975)
                     /locus_tag="OO339_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5927084.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11250..12614)
                     /gene="tdcG"
                     /locus_tag="OO339_002492"
     CDS             complement(11250..12614)
                     /gene="tdcG"
                     /locus_tag="OO339_002492"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5927085.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12686..13075)
                     /locus_tag="OO339_002493"
     CDS             complement(12686..13075)
                     /locus_tag="OO339_002493"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5927086.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13089..15383)
                     /gene="tdcE"
                     /locus_tag="OO339_002494"
     CDS             complement(13089..15383)
                     /gene="tdcE"
                     /locus_tag="OO339_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5927087.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15417..16625)
                     /gene="tdcD"
                     /locus_tag="OO339_002495"
     CDS             complement(15417..16625)
                     /gene="tdcD"
                     /locus_tag="OO339_002495"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5927088.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16651..17982)
                     /gene="tdcC"
                     /locus_tag="OO339_002496"
     CDS             complement(16651..17982)
                     /gene="tdcC"
                     /locus_tag="OO339_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5927089.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18004..18993)
                     /gene="tdcB"
                     /locus_tag="OO339_002497"
     CDS             complement(18004..18993)
                     /gene="tdcB"
                     /locus_tag="OO339_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5927090.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19092..20030)
                     /gene="tdcA"
                     /locus_tag="OO339_002498"
     CDS             complement(19092..20030)
                     /gene="tdcA"
                     /locus_tag="OO339_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5927091.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20220..20564
                     /gene="tdcR"
                     /locus_tag="OO339_002499"
     CDS             20220..20564
                     /gene="tdcR"
                     /locus_tag="OO339_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5927092.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20820..21360
                     /locus_tag="OO339_002500"
                     /pseudo
     CDS             20820..21360
                     /locus_tag="OO339_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21382..21729
                     /locus_tag="OO339_002501"
     CDS             21382..21729
                     /locus_tag="OO339_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927093.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21898..22548
                     /locus_tag="OO339_002502"
     CDS             21898..22548
                     /locus_tag="OO339_002502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927094.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6198 a         5786 c         5290 g         5687 t
ORIGIN      
        1 caatcatcaa atttatatat gaccaatggc atacccatga ataaagtggt atgataactt
       61 cgtccgcatt ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca
      121 acaacttgct gctgacctga aagagcgcat tgaacagggc gtctatctgg tgggtgataa
      181 actgcctgcc gaacgcttta ttgccgatga aaagaacgtc agccgaacgg tagttcgtga
      241 agccatcatc atgctggaag ttgaaggcta tgtggaggtg cgtaaaggtt caggtattca
      301 tgtggtttcc aaccagccgc gtcatcagca ggcggctgac aataatatgg aattcgccaa
      361 ttacggtccg tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt
      421 cgcggcaact caggtaacga aacaagacat catgaaactg atggccattc aggaacaggc
      481 gcgcggcgaa caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc
      541 cctggcgacg cagaactccg ccctggcggc catcgttgaa aaaatgtgga cccagcgtag
      601 ccataacccg tactggaaaa aactacacga acacattgat tcccgtaccg ttgataactg
      661 gtgtgatgac cacgatcaaa ttctcaaggc gttaattcgc aaagatcctc atgctgctaa
      721 gctggcaatg tggcagcatc tggaaaacac caaaatcatg ttatttaacg aaaccagcga
      781 cgatttcgag ttcaatgccg accgctatct gttcgccgaa aacccggtag tgcatctcga
      841 tacggccacc agcggcagca aatgaaattc ctgtccgaca ggcgctgcaa tgcgcctgtt
      901 tgtctcgtcg gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct
      961 cccctgcaat cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg
     1021 acctttgtta caattagatt caatttgaat ttatgctttt gaatgctttc ttatctcacg
     1081 ttttaacagg gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac
     1141 agcaataaca ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag
     1201 gaataatgaa tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa
     1261 accctggcca atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc
     1321 cttgaaaatg gcttgcttcc ggcggccttt ttaccgggcg acagtttact ggtattggtc
     1381 ggcgtgttga ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt
     1441 gccgccagcc tcggttgctg ggtcagctat attcaggggc gatggctggg caatacccgc
     1501 accgtacaaa actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt
     1561 tttcataaac acggtttatc ggcgctgtta attggtcgct ttattgcatt tgtcagaaca
     1621 ctgctgccga cgattgccgg gttatcaggg ctgaataacg cgcgctttca gttcttcaac
     1681 tggatgagcg gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa
     1741 acgccggtat ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg
     1801 gtgctgctgg tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga
     1861 aatcgggggt aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc
     1921 actgttttac ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag
     1981 tctacgctgg cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc
     2041 tggcatcacc ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact
     2101 ctgttaatta catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga
     2161 acattgcccc atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg
     2221 ctgacccggt tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata
     2281 tcactcactt tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact
     2341 accgcttcgt tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt
     2401 caagaatgaa ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt
     2461 atgccactac cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg
     2521 aaaaacacca aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg
     2581 ccaactgttc agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag
     2641 atgaggtggc ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata
     2701 gaacggaaac tggcagaagc gcaggaagag ctgaaaaagc tggaagcgcg cgactactaa
     2761 ctcacaatag tcactactta ctcacctgga gaaaactatg tcgaaagaac acactacgga
     2821 acatctgcgt gctgagttga aatccctttc cgatacgctg gaagaggtgc ttagctcatc
     2881 tggcgagaag tcgaaagaag agttgagtaa gattcgtagc aaagcggagc aggcactgaa
     2941 acagagccgt tatcgcctgg gtgaaaccgg tgatgccatc gccaaacaaa cccgtgtcgc
     3001 ggcggcgcgt gctgatgagt atgtgcgtga aaatccgtgg accggcgtgg gaattggcgc
     3061 tgcaatcggt gtagtgctcg gcgttctgct gtcgcgtcgt taattatggc ggacactcat
     3121 cacgcgcaag ggcccggtaa aagcgttctg ggcatcgggc agcgaattgt ttccatcatg
     3181 gttgaaatgg tggaaacacg tctgcggctg gcggtggtgg agctggaaga ggaaaaagct
     3241 aatctctttc aacttttact gatgctgggc ctgacgatgc ttttcgctgc atttggtctt
     3301 atgagcctga tggtgctaat tatttgggcg gttgatccgc aatatcgcct gaatgcgatg
     3361 attgccacca ccgtggtgtt gctgctactg gcactgattg gcggtatctg gacgttacgt
     3421 aaatcgcgta aatccacctt gctgcgccat acacgccatg agctggcaaa cgatcggcaa
     3481 ctgctcgagg aggagtcccg tgagcagtaa agtcgaacgt gaacgacgta aggcgcaact
     3541 gcttagccag atccagcaac aacggctgga tatttccgcc agtagtcgtg aatggctgga
     3601 ggcaacaggc gcttacgatc gtcgctggaa tatgctgcta agtctgcgct cctgggcgct
     3661 ggttggcagt agagtgatgg cgatctggac gattcgccat cctaatatgc tggtccgctg
     3721 ggccagacgc ggttttggcg tatggagcgc ctggcgtctg gttaaaacga ccctcaagca
     3781 gcaacagctt cgcggttaac ttccctctgg ccggagcaat tccggcctta tccatcaaat
     3841 tttttgaata tttttgacag ttttccttgc taacaatcat cattcaccac gtttatgatt
     3901 ctctccatcg acagcaacga cgctaatagc gcgtccttgc acaaaaaaac aatcagcagc
     3961 ctgagtggcg cagtggagag tatgatgaaa aaattagaag atgttggtgt actggtagcg
     4021 cacattttaa tgccgattct gtttattacc gcaggctggg gaaaaatcac cgcttactct
     4081 ggtacccaac aatatatgga agcaatgggt gtcccgggtt ttatgctgct gacggcattt
     4141 ttatttcaca gcaactttgc tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca
     4201 atttctggcg gattcctgct gctggcaatt accggtccgg gcgcgtatag catcggccgc
     4261 ctgctgaata aaaagtggta agcacgcgct atacttaacg ataaaaaacg aggaggaagc
     4321 tcctcgtttt tgctattgga ggagagaaaa atgggtcaac tgattgacgg cgtctggcat
     4381 gacacctggt acgataccaa atctaccggc ggtaaatttc aacgttcagc ttccgcattt
     4441 cgtaactggc tcactgccga tggcgcacct ggccccactg gcacaggcgg ttttatcgca
     4501 gagaaagatc gttatcatct ctatgtttca ctcgcctgcc cgtgggcgca ccgcacgctg
     4561 atcatgcgca aactcaaagg actggaaccg tttatttccg tttccgtagt gaacccgctg
     4621 atgctggaaa acggctggac ctttgatgac agttttccgg gagcaaccgg agatacgctc
     4681 tatcaacatg aatttctgta tcagctttat ctccacgccg atccacacta cagcggacga
     4741 gttactgttc ccgtgctgtg ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca
     4801 gaaatcatcc gcatgttcaa taccgcgttt gatgcgctgg gcgcaaaagc gggtgattac
     4861 tacccaccag ccctgcaaac gaaaattgac gaacttaacg gctggattta tgacaccgtt
     4921 aacaacggcg tgtataaagc tggttttgcc accagccagc aagcctacga cgaggcggtg
     4981 gcgaaagtgt ttgaatcgct ggcgcgactg gagcagattt taggccagca tcgttacctg
     5041 accggcaacc agctaaccga agccgatatt cgcctgtgga ctacgctggt gcgttttgat
     5101 ccagtgtatg tgacccactt caagtgtgat aagcaccgca tcagcgatta cctgaatctg
     5161 tatggcttcc tgcgcgatat ctaccagatg ccggggatcg ccgaaactgt caatttcgat
     5221 cacatccgta atcattactt ccgcagccat aagaccatca accctacggg gattatttca
     5281 attggtccgt ggcaggatct cgatgaaccg catggacgag atgttcgctt cggttaaaaa
     5341 aaggcacctt acgaggtgcc tctgataatt agctaagtgc atgttgttca tgtcggatgc
     5401 ggcgtgaacg ccttatcaaa cctacaaagc gtttatattc aatatgttac aatgaccgcg
     5461 taggccagat aagcgtagcg catctggcga ttttgcgctt gccatcagtc ttagcgcgcc
     5521 ttcacggcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     5581 cttaatttgc tgctttaaaa acaagtgatt gagcaaaatt gaggcaaaaa tggactggta
     5641 tctgaaagta ctaaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     5701 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     5761 aggctggcaa cgcgctggtg gcgaaggcat cctgacgata atctacggta ttctggtgtt
     5821 tttaccgtgg tgggcggttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     5881 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     5941 ggcgggtacg ccaggcgaaa accgctttgg accagatcca aaactggaac aagaataatg
     6001 attaagcacc ttgtaaaagg tgcttcaaat tgttgacaaa gtgcgctttg tttatgccgg
     6061 atgcggcgta aacgccttat ccagcctaca aattgccaaa attcaatata ttgcagtgac
     6121 tgcgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcagc agtcagaagc
     6181 accttgtgaa aggtgcttaa tagattattt tccagcgaac aactttggaa tttcccgcag
     6241 acaccaggat ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc
     6301 gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat
     6361 cggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat
     6421 cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc
     6481 acgagcggta tccgctaccg caattccacg atatttcaca cgcgtcactt cagataacgg
     6541 ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaacgtata
     6601 gagcttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacgat
     6661 atccgcccgc ccctgctcca gccgttccca cgcccccgcc agcacttctg tgatgattgc
     6721 cagttgggta ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg
     6781 taccagcgct tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gagcttccgc
     6841 atcagtagtc agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct
     6901 cccgacattg gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc
     6961 ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca gctcatccgc
     7021 tgccgccgca aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt
     7081 taatgccctt tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa
     7141 tatctggcta acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa
     7201 gaattatgat tactacccga actgccaggc agtgtggaca agcagactac ggatggttgc
     7261 aggcccggta tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct
     7321 ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag ccgcgaacct
     7381 atcccaaagt cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg
     7441 aaggcaatca tgttcaggcc agcgccggag aggcgttgct gctctctacc cagccgggtg
     7501 tcagctatag cgaacacaat ctcagcaaag acaaaccgtt aacgcgaatg cagctttggc
     7561 tggatacctg cccgcagcga gagaatccgc tgattcaaaa gctggcgctt aatatggaca
     7621 agcagcaatt aatcgcctcg ccagagggtg cgatgggaag cctgcaatta cgccaacaag
     7681 tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc
     7741 cacgcgcgta tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga
     7801 aagcggcgct aacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg
     7861 ttgccgattc cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg
     7921 gagatgatga tgagtaaaaa atcgaccaaa aagcgccagc cagcgaagcc cgtggtggcg
     7981 aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa
     8041 gctatcgtcg cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag
     8101 gttgccggat gcggcgtaaa cgccttatcc ggcctacaaa acacaacaac atcaacaaat
     8161 taaacacccc gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac
     8221 acaacaacat caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc
     8281 cggcacaata ctcaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct
     8341 gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct
     8401 caacatcatg cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta
     8461 acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg
     8521 caccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatag
     8581 tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg
     8641 ttgcggcaca aagtgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat
     8701 gagaaagcat cagcgcacgc gccagtcgtt catcatccgc tccgaagtgt tctgctacaa
     8761 ccaccacagg cattattgca gtgatcccct ggttacccga gccggagtta ctcatagccg
     8821 gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg
     8881 aagagagatc tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat
     8941 gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat
     9001 cgagaataaa gcggatcgcc gcaaatggga cttcattgac gaacttcagg atctcagcca
     9061 gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc gccctctgcc acacacgcct
     9121 ggtgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga
     9181 cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt
     9241 cattgcaagg ttcctggatc ttaacagaga ctttccccgc cgccagcagt gctttggcat
     9301 cggtaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac
     9361 ctaacgcccc cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga
     9421 cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac
     9481 cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc
     9541 ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta
     9601 aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga
     9661 acagagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt
     9721 atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc
     9781 cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc
     9841 tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct
     9901 tctcggcagg catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa
     9961 agcctaaata gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga
    10021 ccactttcac ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg
    10081 aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca
    10141 cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg
    10201 ctttatgccg cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc
    10261 ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt
    10321 tcaccagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca
    10381 tcgacacgcc cagcgcagcg actaccagca gcttggtcag caccatgtcg gtcgaaattt
    10441 tgaataacaa tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac
    10501 cataaaaggg actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag
    10561 cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat
    10621 agagcgcacc taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt
    10681 aatctttaca ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca
    10741 ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct
    10801 gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc
    10861 cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac
    10921 gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt
    10981 ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg
    11041 gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa
    11101 aatcttcaca atcaagactt taaaataacg gagatggata agaattttct actaaattaa
    11161 tcacaagaga atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt
    11221 gcacccaagg atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct
    11281 ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg
    11341 atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg
    11401 tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg
    11461 gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat
    11521 acctgcgccg gactgccgcc cagtagttca gttaaccctg ccgctgccat tgaacaggcc
    11581 acgccaatct ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac
    11641 agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga
    11701 cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc
    11761 ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg
    11821 ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctaga aaccagctga
    11881 cgacgcagcg ctacggcacg acacggcaca ttgagcggac caggcagcac gccttcagtg
    11941 ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca
    12001 atttccgctt tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg
    12061 ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac
    12121 gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca
    12181 gagtaatagg ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg
    12241 ttctcatggc gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca
    12301 acaatatgcg caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat
    12361 gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg
    12421 gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccgtacag atcgaccaca
    12481 atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg
    12541 gcattcattg gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg
    12601 aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc
    12661 ttgcgaccgt cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca
    12721 gcttcacatc tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct
    12781 gatgctcatc gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg
    12841 tgatgaacac ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg
    12901 ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg
    12961 tctgcgggca aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct
    13021 gaacataagg gccgattgcg cctagggcac gttgcgtttc gataatcttt ttcatacatc
    13081 ctccggcgtc agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg
    13141 gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt
    13201 tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt
    13261 tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg
    13321 gttttacgta ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag
    13381 atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca
    13441 cctttgcggt cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga
    13501 cgaccgtccg gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga
    13561 atcgactggg tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag
    13621 cgttcaacca ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga
    13681 tattcaccgt cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg
    13741 cgggcatatt tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat
    13801 gccatagtgc gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac
    13861 ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc
    13921 atgaagtgat cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt
    13981 gctgttttcg gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag
    14041 agcagcgttt tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc
    14101 attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga
    14161 tcgtcatttt catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg
    14221 aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc
    14281 atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc
    14341 agccccatcc cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca
    14401 aattccggtg tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt
    14461 tcctgtgcct gctgctcatt gaggacgcca gctttaaagt cgcgctcaat gtagatatcc
    14521 aggaacgatg ccgtgcgccc cagcgacatc gcgccgccat tttgcgattt cactgccgcc
    14581 agataagcga agtagagcca ctgcaccgct tcctgtgcat tctgcgccgg gcgagagata
    14641 tcaaagccat atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc
    14701 agctcctcac gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg
    14761 agatcggcaa attgcagttc gcgttcacgc accagataac tgatgccata cagcgctacg
    14821 cggcgatagt caccgataat gcgcccacga ccatagccat ctggtaaacc ggtcagcacg
    14881 ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg
    14941 gttttacgca gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg
    15001 tggaatgaac ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc
    15061 gcatccgttt gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga
    15121 gcggtaattg tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcgttttcg
    15181 atacggatgc cttccattac cttttcccac aattccgtgg tggcaggcgt cgcttcggcg
    15241 agaaaagatt catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta
    15301 atttcgcttt tccagtccgt acctttaaag ccaagccatg cgtcagcgta cagcttatcg
    15361 ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca actaaattat
    15421 gcaaattctg cgggcgcgtt aactttacct aaatgaatgg catccaaagc aatcattttt
    15481 tcttcgttag tcggaataac gacgcaaatg acatgcgcat tttcactgga aacaattcgc
    15541 tcgccaaaag agttagaacg attattcatt tctgtatcaa tctctacgcc taatacagcc
    15601 aaatgttcca tgaccagacg acgaattaaa cttgaattct ctcctattcc gccggtgaat
    15661 ataattccat ccagccgacg taatgaagct gcatgtccgg caatatgacg ggcaattcgg
    15721 tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc
    15781 agaacacgta aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact
    15841 acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca
    15901 aagtcgacat cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt
    15961 gaggtgtcaa cactctgacc gttgcgaact gcgcagattg acgcgccatt gccaagatgc
    16021 gccacaacca ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa
    16081 acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat
    16141 ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca
    16201 aataccgcca cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa
    16261 tttgcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca
    16321 tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt
    16381 aaggccacgc tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca
    16441 ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca
    16501 ttttccgagt taataccgtc ggcaatacct gacattaata cttcacaatc gctggcatcg
    16561 agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca
    16621 ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat
    16681 ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag
    16741 agacggcgct ttacggatag catacatcgg caacaggcac agcagggatg cgataatcgg
    16801 tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt
    16861 ggagcccatg atgaagatca tgctgagagt attcagttta cccagcgaca ctttggtttt
    16921 gtcaccttta taaccgaact tcagaatcag accattcaag ccttccagcg tccccagata
    16981 gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg catattccag
    17041 tgtaatcgcg aacgttgttt tcgtaccggt catggacgca aagtggttag ccagataaga
    17101 aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca gagtaaacag
    17161 gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat
    17221 ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt
    17281 agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat
    17341 ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc
    17401 aacctggtcg ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag
    17461 gctggcgata aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa
    17521 ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg
    17581 tgcaaatccg agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata
    17641 aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt
    17701 tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag
    17761 acacagacgc gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat
    17821 cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc
    17881 gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga
    17941 ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat
    18001 taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc
    18061 ggaaataata ctgacggttt ttctattttg aatatattgg tctaatttac cgcttaataa
    18121 tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc
    18181 aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg
    18241 aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc
    18301 ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt
    18361 ttctgactgt acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat
    18421 accagcaatt aaaccaccac caccaattgg cacaatcacg ttatcgacat catagagatc
    18481 ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg
    18541 tgggataaaa atacgacctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt
    18601 gaagttatca ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga
    18661 ttttggcgca ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag
    18721 cgaaacccct tgcgcatggt tgcccgcaga acaggccacc acgcctttgc gtttttccgc
    18781 atcggtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg
    18841 catattttca aacttcagga atatttcacc tttgcaacgt tcactaaaat agttggagcg
    18901 aggcatgcct gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatatcatc
    18961 aatagcaacc ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat
    19021 atgtaggtaa ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata
    19081 aaaacaaaca actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa
    19141 tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac
    19201 cataccgcgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga
    19261 ttagaaccaa aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga
    19321 acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca
    19381 tttctttgta acgtagtaag cagttcgctg tagtacccca tattagtttg tggcaacacc
    19441 cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg
    19501 gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt
    19561 tctgcactta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac
    19621 gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg
    19681 ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc
    19741 accaccgctt cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta
    19801 atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt
    19861 ttccgcacca ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg
    19921 gccggttgag ttaaccctaa ttcttttgca gccgagccga tagagccact tctaatgact
    19981 tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac
    20041 gttaattacc tcattgacgg catgaagtgt atcaaaatga aataaacagg atatgtgcga
    20101 ccactcacaa attaactttc attactttcc aaggtatcgt tattacaatt aaacatcttt
    20161 aaatatcaac aagttaaagc ataaaaatca gcataaaccc tgatttttat caaaaaaaaa
    20221 tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa
    20281 aggggctgag accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa
    20341 atttaatgag caagttctca aattttatta taaataaacc attttcagcg ataaatactg
    20401 cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt
    20461 ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct
    20521 cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat
    20581 aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg gtcgccgcga
    20641 taaggtcaga cgaagtcaac aagggggatt ttcactgtgc tttacatcag cgtaggacac
    20701 agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgtaag
    20761 tgtcgtgcga ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa
    20821 tgaaaggctt cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg
    20881 ttaacaatca caatagaaat attgattatt ggacggtaaa aagaaagttt gcagaaattg
    20941 tctccaccaa tgacgttaat aaaatttaca gtataagtaa tgaactccgg agagtattat
    21001 ctgcaattac tgcattgaat ttctatcaag gcgatgttcc ttctgtcatg attccgaatc
    21061 caaccggaaa atatgagtcc attcatcata gatatttcta caggagaaca tgatgattat
    21121 atcatacaaa cattagatgt aggcactttt gcaccttttg gtgaacaatg tacttgctca
    21181 gccgtcaata aaaaagagct ggaatgtatt aaggagacga tttctaagta ttgcgcaaag
    21241 ttcacccaaa aagaagccat cttaacaccc cctaggcact ttaataaaac ctgcattact
    21301 tcagattgtt ggcaaattct ctttttttca cccgatcatt ttaataatga tttttattga
    21361 ttatttataa aggataaaat aatgttccct gtttcatcaa ttggtaatga tattagtagt
    21421 gatttagtcc gtcgaaaaat gaatgacctt cctgaaagcc ccataggaaa taacctcgaa
    21481 gcactagcac caggtataga aaaactaaaa cagacctcta ttcaaatggt cactttactt
    21541 aacgcgttac aaccaagtgg aaaatgcatt atcactggtg attttcaaaa agaattagcg
    21601 tacttacaaa atgtaattct ttataatgac tcgtctcttc gtctggattt tttaggttat
    21661 aacacccaaa ttattcaacg atcggacaat acctgcgaac ttaccattaa tgaaccgtta
    21721 aaaaactagg aaatatctac aggcaatatc aatgttaatt gcccattaaa agatatttac
    21781 aatgaaatca ggaggttgaa cgtaatcttt agttgtggga ctggagatat agttgatcta
    21841 tcctctctgg acttacgtaa tgtcgattta gattattatg atttcacaga taaacatatg
    21901 gctaatacta ttctaaatca ttttaaattg gattcaacaa attttactaa tgccaacatg
    21961 tttcaggtta attttgttag ttcaacacaa aacgccacaa tctcctggga ttatttacta
    22021 aaaataacgc ctgttttaat aagcattagc gatatgtatt ctgaagaaaa aatcaagttt
    22081 gtcgaaagtt gtttaaatga gcttggagac attaccgaag aacaattaaa aattatgaga
    22141 tttgcaatta taaaatctat accaagggca actcttacag ataaattaga aaatgaatta
    22201 acaaaagaaa tatataaaag ctcatcgaaa atcaacaatt gcttgaacag aattaaatta
    22261 ccagagatga aagaattctc atcaaaaaaa acatatgatt acatcgatac aatcattgaa
    22321 gattatgaaa atatcaaaga aaatgcttat ctggtcgtcc cccaaattaa ttatactatg
    22381 gatttaaaca tagaagactc tagctcagaa gagttaatac cctcgagaaa gacgaaaatt
    22441 ctccggacaa tggctttgag gtcggggaat ataacacata tgaagcatat aactcagaga
    22501 atcaatattt taccagagag gactatacgt ataattacga ccttttaaat gcaatataga
    22561 cttaataact tcttacaaag tctattatta ataccgtaga tattcatctt accctgtgca
    22621 ccaaaagcaa aaacttatcg acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga
    22681 tgcgctacgc ttatcaggcc tacgccatct ctgcaatata ttgaatttgc gtgcttttgt
    22741 aggcaggata aggcgttcac gcagcatccg gcatgaataa agcgcactca acaatctgaa
    22801 acccgccgga acggtttatt acgtacatca ggtgaaactg accgataagc cgctttcttt
    22861 tgggtatagt gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc
    22921 gaagcgggtt tttacgtata tcaggtgaaa ctgaccgata a
//
LOCUS       DAJTRH010000059        22776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTRH010000059 DAJTRH010000000
VERSION     DAJTRH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..1679
                     /gene="thrS"
                     /locus_tag="OO339_002503"
     CDS             <1..1679
                     /gene="thrS"
                     /locus_tag="OO339_002503"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5927095.1"
                     /translation="QLWPHTKMAIGPVIDNGFYYDVDLDRTLTQEDVEALEKRMHELA
                     EKNYDVIKKKVSWHEARETFANRGESYKVSILDENIAHDDKPGLYFHEEYVDMCRGPH
                     VPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRIYGTAWADKKALNAYLQRLEEAAKRDH
                     RKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRELEVFVRSKLKEYQYQEVKGPFMMDRV
                     LWEKTGHWDNYKDAMFTTSSENREYCIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGS
                     CHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQIRDEVNGCIRLVYDMYSTFGFEKIVV
                     KLSTRPEKRIGSDEMWDRAEADLAVALEENNIPFEYQLGEGAFYGPKIEFTLYDCLDR
                     AWQCGTVQLDFSLPSRLSASYVGEDNERKVPVMIHRAILGSMERFIGILTEEFAGFFP
                     TWLAPVQVVIMNITDSQSEYVNELTQKLSNAGIRVKADLRNEKIGFKIREHTLRRVPY
                     MLVCGDKEVESGKVAVRTRRGKDLGSMDVNEVIEKLQQEIRSRSLKQLEE"
     gene            1683..2225
                     /gene="infC"
                     /locus_tag="OO339_002504"
     CDS             1683..2225
                     /gene="infC"
                     /locus_tag="OO339_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5927096.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            2322..2519
                     /gene="rpmI"
                     /locus_tag="OO339_002505"
     CDS             2322..2519
                     /gene="rpmI"
                     /locus_tag="OO339_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5927097.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            2572..2928
                     /gene="rplT"
                     /locus_tag="OO339_002506"
     CDS             2572..2928
                     /gene="rplT"
                     /locus_tag="OO339_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5927098.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3045..3169
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3051..3095
                     /gene="pheM"
                     /locus_tag="OO339_002507"
     CDS             3051..3095
                     /gene="pheM"
                     /locus_tag="OO339_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5927099.1"
                     /translation="MNAAIFRFFFYFST"
     gene            3378..4361
                     /gene="pheS"
                     /locus_tag="OO339_002508"
     CDS             3378..4361
                     /gene="pheS"
                     /locus_tag="OO339_002508"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5927100.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            4376..6763
                     /gene="pheT"
                     /locus_tag="OO339_002509"
     CDS             4376..6763
                     /gene="pheT"
                     /locus_tag="OO339_002509"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5927101.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            6768..7067
                     /gene="ihfA"
                     /locus_tag="OO339_002510"
     CDS             6768..7067
                     /gene="ihfA"
                     /locus_tag="OO339_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5927102.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7168..8154
                     /gene="btuC"
                     /locus_tag="OO339_002511"
     CDS             7168..8154
                     /gene="btuC"
                     /locus_tag="OO339_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5927103.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRFRLLLAGVALGIICSA
                     LMTWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLAL
                     GEISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRV
                     LLPGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8217..8768
                     /gene="btuE"
                     /locus_tag="OO339_002512"
     CDS             8217..8768
                     /gene="btuE"
                     /locus_tag="OO339_002512"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5927104.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            8768..9517
                     /gene="btuD"
                     /locus_tag="OO339_002513"
     CDS             8768..9517
                     /gene="btuD"
                     /locus_tag="OO339_002513"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5927105.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            9595..10059
                     /locus_tag="OO339_002514"
     CDS             9595..10059
                     /locus_tag="OO339_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5927106.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            10306..11019
                     /gene="ydiV"
                     /locus_tag="OO339_002515"
     CDS             10306..11019
                     /gene="ydiV"
                     /locus_tag="OO339_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5927107.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11083..12519
                     /locus_tag="OO339_002516"
     CDS             11083..12519
                     /locus_tag="OO339_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5927108.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(12523..12714)
                     /gene="hemP"
                     /locus_tag="OO339_002517"
     CDS             complement(12523..12714)
                     /gene="hemP"
                     /locus_tag="OO339_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5927109.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(12846..13892)
                     /gene="aroH"
                     /locus_tag="OO339_002518"
     CDS             complement(12846..13892)
                     /gene="aroH"
                     /locus_tag="OO339_002518"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5927110.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14049..14882)
                     /gene="ppsR"
                     /locus_tag="OO339_002519"
     CDS             complement(14049..14882)
                     /gene="ppsR"
                     /locus_tag="OO339_002519"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5927111.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            15214..17592
                     /gene="ppsA"
                     /locus_tag="OO339_002520"
     CDS             15214..17592
                     /gene="ppsA"
                     /locus_tag="OO339_002520"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5927112.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(17649..19295)
                     /gene="fadK"
                     /locus_tag="OO339_002521"
     CDS             complement(17649..19295)
                     /gene="fadK"
                     /locus_tag="OO339_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5927113.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(19352..19645)
                     /locus_tag="OO339_002522"
     CDS             complement(19352..19645)
                     /locus_tag="OO339_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5927114.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(19642..20931)
                     /locus_tag="OO339_002523"
     CDS             complement(19642..20931)
                     /locus_tag="OO339_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5927115.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(20987..21925)
                     /locus_tag="OO339_002524"
     CDS             complement(20987..21925)
                     /locus_tag="OO339_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5927116.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(21945..22709)
                     /locus_tag="OO339_002525"
     CDS             complement(21945..22709)
                     /locus_tag="OO339_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5927117.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5590 a         5599 c         6036 g         5551 t
ORIGIN      
        1 aacaactttg gccgcatacc aaaatggcaa tcggcccggt tattgacaac ggtttttatt
       61 acgacgttga tcttgaccgc acgttaaccc aggaagatgt cgaagcactc gagaagcgga
      121 tgcatgagct tgctgagaaa aactacgacg tcattaagaa gaaagtcagc tggcacgaag
      181 cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt ttccattctt gacgaaaaca
      241 tcgcccatga tgacaagcca ggtctgtact tccatgaaga atatgtcgat atgtgccgcg
      301 gtccgcacgt accgaacatg cgtttctgcc atcatttcaa actaatgaaa acggcagggg
      361 cttactggcg tggcgacagc aacaacaaaa tgttgcaacg tatttacggt acggcgtggg
      421 cagacaaaaa agcacttaac gcttacctgc agcgcctgga agaagccgcg aaacgcgacc
      481 accgtaaaat cggtaaacag ctcgacctgt accatatgca ggaagaagcg ccgggtatgg
      541 tattctggca caacgacggc tggaccatct tccgtgaact ggaagtgttt gttcgttcta
      601 aactgaaaga gtaccagtat caggaagtta aaggtccgtt catgatggac cgtgtcctgt
      661 gggaaaaaac cggtcactgg gacaactaca aagatgcaat gttcaccaca tcttctgaga
      721 accgtgaata ctgcattaag ccgatgaact gcccgggtca cgtacaaatt ttcaaccagg
      781 ggctgaagtc ttatcgcgat ctgccgctgc gtatggccga gtttggtagc tgccaccgta
      841 acgagccgtc aggttcgctg catggcctga tgcgcgtgcg tggatttacc caggatgacg
      901 cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt taacggatgt atccgtttag
      961 tctatgatat gtacagcact tttggcttcg agaagatcgt cgtcaaactc tccactcgtc
     1021 ctgaaaaacg tattggcagc gacgaaatgt gggatcgtgc tgaggcggac ctggcggttg
     1081 cgctggaaga aaacaacatc ccgtttgaat atcaactggg tgaaggcgct ttctacggtc
     1141 cgaaaattga atttaccctg tatgactgcc tcgatcgtgc atggcagtgc ggtacagtac
     1201 agctggactt ctccttgccg tctcgtctga gcgcctccta tgtgggcgaa gacaacgagc
     1261 gtaaggtacc ggtaatgatt caccgcgcaa ttctggggtc gatggagcgt ttcatcggta
     1321 tcctgaccga agaattcgct ggtttcttcc cgacctggct tgcgccagtt caggttgtta
     1381 tcatgaatat taccgattca cagtctgaat acgttaacga attgacgcaa aaactatcaa
     1441 atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa gattggcttt aaaatccgcg
     1501 agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg tgataaagag gtggaatcag
     1561 gcaaagttgc cgttcgcacc cgccgtggta aagacctggg aagcatggac gtaaatgaag
     1621 tgatcgagaa gctgcaacaa gagattcgca gccgcagtct taaacaattg gaggaataag
     1681 gtattaaagg cggaaaacga gttcaaacgg cgcgccctaa ccgtatcaat ggcgaaattc
     1741 gcgcccagga agttcgctta acaggtctgg aaggcgagca gcttggtatt gtgagtctga
     1801 gagaagctct ggagaaagca gaagaagccg gagtagactt agtcgagatc agccctaacg
     1861 ccgagccgcc ggtttgtcgt ataatggatt acggcaaatt cctctatgaa aagagcaagt
     1921 cttctaagga acagaagaaa aagcaaaaag ttatccaggt taaggaaatt aaattccgtc
     1981 ctagtacaga tgaaggcgac tatcaggtaa aactccgcag cctgattcgc tttctcgaag
     2041 agggtgataa agccaaaatc acgctgcgtt tccgcggtcg tgagatggcg caccagcaaa
     2101 tcggtatgga agtgcttaat cgcgtgaaag acgatttgca agaactggca gtagtcgaat
     2161 ccttcccaac gaagatcgaa ggccgccaga tgatcatggt gctcgctcct aagaagaaac
     2221 agtaaggcct tcaagtagca ctatctgtgg agcctttggg cttcacaggc attgttcgcc
     2281 tttgtttcgt ttattaacaa tgcgaagtgg aagttattaa aatgccaaaa attaagaccg
     2341 tacgcggtgc tgctaagcgc ttcaaaaaaa ccggtaaagg tggttttaag cacaagcacg
     2401 ctaacctgcg tcacattctg actaaaaaag cgaccaaacg taaacgtcac ctgcgtccga
     2461 aagccatggt ttccaaaggc gatctgggcc tggtaatcgc gtgcctgccg tacgcataag
     2521 ccgttaacgt ttttaacttt ttaattagaa tatagataca ggagagcaca tatggctcgc
     2581 gtaaaacgtg gtgttattgc acgcgcacgt cacaagaaaa ttttgaaaca agctaaaggc
     2641 tactacggtg cgcgttctcg cgtataccgc gttgccttcc aggctgttat caaagctggt
     2701 cagtatgctt accgtgaccg tcgtcaacgt aagcgtcagt tccgtcaact gtggattgcg
     2761 cgtatcaacg cagcagcacg tcagaacggt atttcttaca gcaaattcat caatggcctg
     2821 aaaaaagcct ctgttgaaat cgaccgtaag atcctggctg atatcgcagt attcgacaaa
     2881 gtagcgttca ccactctggt tgaaaaagcg aaagcagctc tggcataagc cagttgaaag
     2941 agggagctag tctccctctt ttcgtttcaa cgccatcaaa acattgactt ttatcgccgt
     3001 agccttttca ataaaggtct tttgaagagt accaaaaggt aacgcaagca atgaatgctg
     3061 ctattttccg cttctttttt tactttagca cctgaatcca ggaggctagc gcgtgagaag
     3121 agaaacggaa aacagcgcct gaaagcctcc cagtggaggc tttttttgta tgcgcgtttt
     3181 gaaaaataaa aagccagtct atcaggcgta attgtcgcag ccagtctgga cacggccagt
     3241 gcggagaaac cggagcgtac ttaagtacgt gaggatttcg agcactgccc gggaccaaaa
     3301 tggcaagtaa aatagcctga tgggataggc tctaagtcca acgaaccagt gtcaccactg
     3361 acacaatgag gaaaaccatg tcacatctcg cagaactggt tgccagtgcg aaggcggcca
     3421 ttagccaggc gtcagatgtt gccgcgttag ataatgtgcg cgtcgaatat ttgggtaaaa
     3481 aagggcactt aacccttcag atgacgaccc tgcgtgagct gccgccagaa gagcgtccgg
     3541 cagctggtgc ggttatcaac gaagcgaaag agcaggttca gcaggcgctg aatgcgcgta
     3601 aagcggaact ggaaagcgct gcactgaatg cgcgtctggc ggcggaaacg attgatgtct
     3661 ctctgccagg tcgtcgcatt gaaaacggcg gtctgcatcc ggttacccgt accatcgacc
     3721 gtatcgaaag tttcttcggt gagcttggct ttaccgtggc aaccgggccg gaaatcgaag
     3781 acgattatca taacttcgat gctctgaaca ttcctggtca ccacccggcg cgcgctgacc
     3841 acgacacttt ctggtttgac gctacccgcc tgctgcgtac ccagacctct ggcgtacaga
     3901 tccgcaccat gaaagcccag cagccaccga ttcgtatcat cgcgcctggc cgtgtttatc
     3961 gtaacgacta cgaccagact cacacgccga tgttccatca gatggaaggt ctgattgttg
     4021 ataccaacat cagctttacc aacctgaaag gcacgctgca cgacttcctg cgtaacttct
     4081 ttgaggaaga tttgcagatt cgcttccgtc cttcctactt cccgtttacc gagccttctg
     4141 cagaagttga cgtcatgggt aaaaacggta aatggctgga agtgctgggc tgcgggatgg
     4201 tgcatccgaa cgtgctgcgt aacgttagca tcgacccgga agtttactct ggtttcgcct
     4261 tcgggatggg gatggagcgt ctgactatgt tgcgttacgg cgtcaccgac ctgcgttcat
     4321 tcttcgaaaa cgatctgcgt ttcctcaaac agtttaaata aggcaggaat agattatgaa
     4381 attcagtgaa ctgtggttac gcgaatgggt gaacccggcg atcgatagcg atacgctggc
     4441 gaatcaaatc actatggcgg gcctggaagt tgacggtgta gaaccggttg ccggtagctt
     4501 ccacggcgtg gtcgttggtg aagtggttga gtgtgcgcag catccgaacg ctgacaaact
     4561 gcgtgtgaca aaagtgaatg tcggcggcga tcgcctgctg gacatcgtct gcggtgcgcc
     4621 aaactgccgt cagggcctgc gtgtggcggt agctaccatt ggtgctgttc tgccgggtga
     4681 tttcaaaatt aaagcggcga aactgcgtgg cgaaccgtct gaagggatgc tgtgctcctt
     4741 ttctgagctg ggaatttctg acgatcacag tggcattatc gaactgcctg cggatgcgcc
     4801 gattggcacc gacatccgcg aatacctgaa actcgatgat aacaccatcg aaatcagcgt
     4861 gacaccaaac cgtgccgact gcttaggtat cattggtgtt gcgcgtgacg ttgccgtgct
     4921 gaatcagctg ccgctggttg aaccggaaat cgttccggtt ggtgcgacta tcgacgacac
     4981 gttgccgatt acagtcgaag cgccggaagc ctgcccgcgc tatcttggcc gtgtggtaaa
     5041 aggcattaac gttaaagcgc caactccgct gtggatgaaa gaaaaactgc gtcgttgcgg
     5101 gatccgttct atcgatgcag ttgttgacgt caccaactat gtactgctcg aactgggcca
     5161 gccgatgcac gctttcgata aagatcgcat tgaaggcggc attgtggtgc ggatggcaaa
     5221 agagggcgaa acgctggtgc tgcttgatgg cactgaagcg aagctgaatg ctgacactct
     5281 ggtcatcgcc gaccacaaca aggcgctggc gatgggcggc atctttggtg gcgaacactc
     5341 tggcgtgaat gacgaaacgc aaaacgtctt gctggaatgc gctttcttca gcccgctgtc
     5401 tatcaccggt cgtgctcgtc gtcatggcct gcatactgat gcgtctcacc gttatgagcg
     5461 tggcgttgat ccggtactgc agcacaaagc gatggaacgt gcgacccgtc tgctgatcga
     5521 catctgcggt ggtgaggctg gcccggtaat tgatatcacc aacgaagcaa cgctgccgaa
     5581 gcgtgcaacc atcactctac gtcgtagcaa actggatcgc ctgataggcc atcatattgc
     5641 tgatgagcag gtaactgaca ttctgcgtcg tctcggctgc gaagtgaccg aaggcaaaga
     5701 cgaatggcag gcagttgcgc cgagctggcg tttcgatatg gagattgaag aagatctggt
     5761 cgaagaagtc gcgcgtgttt acggctacaa caacatcccg gatgagccgg tacaggcaag
     5821 cctgattatg ggtactcacc gtgaagctga cctgtcgctc aagcgcgtga aaacgctgct
     5881 caacgacaaa ggctatcagg aagtgatcac ctacagcttc gttgatccga aagtgcagca
     5941 gatgatccat ccaggcgttg aagccttact gctggcaagc ccgatctctg ttgaaatgtc
     6001 agcaatgcgt ctttctctgt ggaccggcct gctggcaacc gtggtgtaca accagaaccg
     6061 tcagcagaac cgtgtgcgca ttttcgaaag cggtctgcgt ttcgtaccag atactcaggc
     6121 accgttgggc attcgtcagg atctgatgtt agccggtgtg atttgcggta accgttacga
     6181 agagcactgg aacctggcaa aagagaccgt tgatttctat gatttgaaag gcgaccttga
     6241 atccgttctc gacctgaccg gtaaactgaa tgaggttgag ttccgtgcag aagcgaatcc
     6301 ggcactgcat ccggggcaat ccgcagcgat ttatctgaaa ggtgaacgta ttggttttgt
     6361 tggagttgtt catcctgaac tggaacgtaa actggatctt aacggtcgca ctctggtgtt
     6421 cgaactggag tggaacaagc tcgcagaccg cgtggtgcct caggcgcgcg agatttctcg
     6481 cttcccggcg aaccgtcgtg acatcgcggt ggtggtcgca gaaaacgttc ccgcagcgga
     6541 tattttatcc gaatgtaaga aagttggcgt aaatcaggta gttggcgtaa acttatttga
     6601 cgtgtaccgc ggtaagggtg ttgcggaggg gtataagagc ctcgccataa gcctgatcct
     6661 gcaagatacc agccgtacac tcgaagaaga ggagattgcc gctaccgtcg ccaaatgtgt
     6721 agaggcatta aaagagcgat tccaggcatc attgagggat tgaacctatg gcgcttacaa
     6781 aagctgaaat gtcagaatat ctgtttgata agcttgggct tagcaagcgg gatgccaaag
     6841 aactggttga actgtttttc gaagagatcc gtcgcgctct ggaaaacggc gaacaggtga
     6901 aactctctgg ttttggtaac ttcgatctgc gtgataagaa tcaacgcccg ggacgtaacc
     6961 cgaaaacggg cgaggatatt cccattacag cacggcgcgt ggtgaccttc agacccgggc
     7021 agaagttaaa aagccgggtc gaaaacgctt cgcccaaaga cgagtaatct gatctaacta
     7081 aaaaggccgc tatgcggcct tttttctttt cactgtcgaa gagtcaccgt aaaatcaacg
     7141 ccatgacact tcagcagaac ggataccatg ctgacacttg cccgccaaca acagcgacaa
     7201 aatattcgct ggttattatg cctgtcagtt ttgatgctgc tggcgcttct cttaagcctt
     7261 tgcgccggtg aacaatggat ttcgccaggt gactggttta ctcctcgtgg cgaactgttc
     7321 gtctggcaaa ttcgcctgcc acgtacgctg gctgtattgc tggttggtgc ggcgctggct
     7381 atatccggcg ctgtaatgca ggcgttgttt gaaaatcctc tggcagaacc tggactactt
     7441 ggcgtctcta acggcgcagg cgtggggctt atcgccgcgg tattgcttgg gcaagggcaa
     7501 ctccccaact gggcgctagg gctgtgtgcg attgctggcg cgcttatcat cactttaata
     7561 ctcttacgtt tcgcccgtcg tcatctttcg accagtcggt ttcggttatt gctggctggc
     7621 gttgcattag ggattatctg cagcgcacta atgacgtggg ctatctactt ttccacctct
     7681 gttgatttac gtcagctgat gtactggatg atgggcggtt ttggcggcgt agactggcgg
     7741 caaagctggc tgatgctggc attgatcccc gtgttgttgt ggatctgttg tcagtccagg
     7801 ccgatgaata tgttagcgct tggcgagatc tcggcgcggc aactgggttt acccatgtgg
     7861 ttctggcgca atgtgctggt ggcagcgacc ggctggatgg ttggcgtcag tgtggcgctg
     7921 gcgggtgcta tcggctttat tggtctggtg atcccacata ttcttcggtt gtgtggttta
     7981 accgatcatc gcgtattact tcccggctgc gcgctggcag gggcgagcgc attgctgctg
     8041 gccgatattg tagcgcgcct ggcattagct gccgcagagc tgcctattgg cgtggtcacc
     8101 gcaacgttgg gtgcgccggt gtttatctgg ttattgttaa aagcaggacg ttagccgcaa
     8161 aaagacggtc tatgattaaa agctaagatt tttactgacc acacctagga gaaacgatgc
     8221 aagattccat tctgacgacc gtagtgaaag atatcgacgg tgaagtgacc acgctggaga
     8281 agtacgccgg taatgtgctg ttaattgtca atgtcgcctc aaagtgtggc ttaacgccgc
     8341 aatatgagca gttggagaat attcaaaaag cctgggccga tcgaggtttt gtggtgctgg
     8401 gattcccgtg caaccagttt ctggaacaag aaccgggcag cgatgaagag attaaaactt
     8461 actgtaccac cacatggggg gtgacgttcc cgatgttcag taagattgaa gttaatggcg
     8521 aaggacgcca tccgctgtat caaaaattga ttgccgcagc gccgaccaca gtcgcgccgg
     8581 aagagagcgg attctatgcc cgtatggtca gcaaaggccg tgcaccgctg tacccggatg
     8641 atattttatg gaattttgaa aaattcctgg ttggcaggga cggaaaagtc atccagcgtt
     8701 tttccccgga tatgacgccg gaagatccca ttgtgatgga aagcattaaa ctggcgttgg
     8761 caaaataatg tctattgtga tgcagttaca ggatgttgcg gaatctaccc gcctggggcc
     8821 gctttcaggc gaggttcggg ctggggaaat cctgcacctg gtggggccga atggcgcggg
     8881 taagagtacc ttactggcgc gaatggccgg aatgaccagc ggtaagggaa gcattcagtt
     8941 cgcggggcaa ccactggaag catggtccgc aacaaaactc gcgctgcatc gcgcctatct
     9001 ttcacaacag cagacgccgc cgtttgcaat gccggtctgg cactacctga cactgcatca
     9061 gcacgataaa acgcgtaccg aactactgaa tgatgtcgca ggggcgctgg ctcttgatga
     9121 caaactcggt cgtagcacca atcaactttc cggcggtgaa tggcaacgcg tacgtcttgc
     9181 tgcggtggtg ttgcaaatca caccacaagc caatcccgca ggccaattgc tgcttcttga
     9241 tgagccgatg aacagtcttg atgttgcgca acaaagtgcg ttagacaaaa ttctgagcgc
     9301 gctgtgtcag caaggactgg cgattgtgat gagcagtcac gatctcaacc acacgttgcg
     9361 tcatgcgcat cgggcgtggt tgctaaaagg tggaaaaatg ctggccagtg gacgcagaga
     9421 agaggtgctc acgccgccaa atctggcgca ggcctatggg atgaattttc gccgtctgga
     9481 tatcgaaggt cacagaatgc tgatttcgac catctgattt cattagtatc ccgaaaatcg
     9541 ggtaattacg acgctaaatt aatgcaagaa taaaaaacag aggaaccgta cggaatgcgt
     9601 ttctgcctta ttttgatcac agcactgttt ctggccgggt gtagccacca taaagcaccg
     9661 ccgccaaatg ccagactttc tgattcgatt accgttattg ccggtttgaa cgaccagcta
     9721 caaagctggc atggcacgcc gtatcgttat ggtggcatga cgcggcgcgg tgtggactgt
     9781 tcgggatttg tggttgtgac gatgcgcgat cgtttcgatt tgcatctgcc ccgtgaaacc
     9841 aaacaacaag cctctattgg cacgcaaatt gataaagacg agttgctgcc tggtgacctg
     9901 gtctttttca aaacgggttc cggacaaaat ggtttgcatg taggtattta cgataccaac
     9961 aaccaattta tccacgcctc taccagcaag ggagtgatgc gttcctcact tgataatgtc
    10021 tattggcaga aaaatttctg gcaggcgaga cgaatctagt atgtcgaaaa attgacggcg
    10081 atgatacatc gccgtcaatt tactcctgaa tacagtagta ttttgcagac aaaaattccg
    10141 taaatggatt attcgtttat aatcaggata atctaaaaaa aggccccctg aaaaagtggt
    10201 taacatggaa caaatgaaag ttactccttc tgtaattgtt atgtcttatg gcctcatccc
    10261 ttttagccgg atactgaaaa acatccttcg agagggacgg ttaccatgaa gatttttttg
    10321 gagaatcttt atcattctga ttgctatttc ctgccgataa gagataacca gcaactcctt
    10381 gtcggcgttg agttaattac ccatttctcc agtgaagatg gcacggttcg tatcccgacg
    10441 agtcgggtta tagcgcaact taccgaggag caacattggc aattattttc agagcagttg
    10501 gaattactaa aatcatgcca acactttttt attcaacata aactctttgc ctggcttaat
    10561 ttaacgccac aagtggctac attattatta gacagggata attacgcagg cgaactatta
    10621 aaatatccgt ttattgaact gttgattaat gagaattacc cgcatctcaa tgaaggaaaa
    10681 gataatcgcg atcttttgtc cttatcgcaa atgtatcccc ttgtgttagg caatttggga
    10741 gcaggtaata gcacaatgaa agcggttttt gatggtcttt tcacccgcgt aatgctggag
    10801 aaaggtttta ttcagcaaca aatcacgcat cgctccttcg aaccgtttat tcgtgcgatc
    10861 caggcgcaaa tttccccttg ttgcaattgc atcatcgcgg ggggcattga tacgccagaa
    10921 atattggcac agattacccc cttcgatttc catgctctcc agggatgcct gtggcctgcc
    10981 gttcctataa atcagataac gacgttggtt cagcgataac cctcctgttt gctggtgttt
    11041 aagacgagag taaccgtcta cactatcaaa cagataggat ctatgaccct gtcttttatt
    11101 acccgctggc gcgatgaatt gccagaaacc tatacagcac tttcccctac gcctttaaat
    11161 aatgcccggc tgatttggca taataccgaa ctcgctaaca cgctgagtat tccatcgtcg
    11221 ctgtttaaaa atgccgcagg tgtctggggc ggcgaaaccc tactgcctgg tatgtcacca
    11281 ctggcacagg tttacagtgg tcatcagttc gtcgtctggg cgggccaact gggtgatggg
    11341 cgcggcattt tacttggcga acaactgctt gctgatggca ctacaatgga ctggcatctg
    11401 aaaggtgctg gcctgacgcc ttattcgcga atgggtgatg gacgggcggt tttacgttcg
    11461 acgatacgag aaagtctcgc cagtgaggcg atgcattatc tgggcattcc gacgacccgc
    11521 gcgttaagta tcgtcaccag cgattcgcca gtgtatcggg aaacggtgga gccaggcgcg
    11581 atgctgatgc gtgtggcacc aagtcatctg cgctttggtc atttcgaaca tttttactat
    11641 cgccgcgagc cggaaaaggt tcgtcagttg gctgactttg ccattcgtca ttactggtca
    11701 catcttgaag acgatgagga caaataccgt ctctggttta acgatgttgt cgcacgtacc
    11761 gcgtcgttaa ttgcccaatg gcagacggtc ggctttgcac atggggtgat gaataccgac
    11821 aacatgtcgc tgctgggact gacgcttgat tacgggccgt ttggttttct tgatgattac
    11881 gaacccggtt ttatttgtaa tcactcggat catcaagggc gttatagctt tgataatcaa
    11941 cctgctgtcg cgttgtggaa tttacagcgt ctggcgcaga cattgtcacc atttgttgcc
    12001 gtagatgccc tgaatgaggc cctggacagc tatcagcagg ttttgttgac gcattatgga
    12061 caacggatgc ggcagaaact gggcttcatg acggagcaaa aagaggataa cgcgctactg
    12121 aatgaattat tcagtctgat ggcgcgagag cgcagcgatt atacccgcac attccgcatg
    12181 ctgagtctga ccgagcagca cagcgcggcg tcaccgctac gtgaggagtt tattgatcgt
    12241 gcggcatttg atgactggtt tgcccgttat cgggggcgtt tgcaacaaga cgaggttagc
    12301 gatagtgagc gtcagcaact gatgcaaagc gttaacccag ctctggtatt gcgcaactgg
    12361 ttggcgcaac gggcgattga agcggcagaa aagggtgata tgatggaatt gcaccgcctg
    12421 catgaggcgt tgcgaaatcc tttcagcgac agagatgatg actatgtcag tcgtccacct
    12481 gactggggta aacggctgga agtcagctgc tcgagttaaa ggctacttag ttaacagcag
    12541 cttgccagcc tgagttttac ggagcagata ttcttgcccg tcatgatcga taatcagttt
    12601 gccatccgga cctaacagtg tctggctggt aattcgccgg atgggctgcg gggaagcggt
    12661 cttgtgattg gcatccgttt caggcgtgag ttttctgcta tccgtataac gcataaaaat
    12721 taataataat tgatataaca atgattccta ttatcaatat tatggcagac cagagcaagc
    12781 agtaatgtgt tgagaagaca acaagttgcg cagaaatgcc catcccatca ggaatgggca
    12841 cgcattcaga agcgggtatc taccgcaaag gcgagttttt cgaccaggcg ttcggtatct
    12901 tcccagccca gacacgggtc ggtaatggat tgaccgtaag tgagcggctg accgccgacg
    12961 attttttgcg ttccttcgcg caggaaactt tccgccataa ttccagcaat ctccgtagag
    13021 ccattgcgga tttgctgaca aatatcctca caaacttcta actggcgacg gtgctgcttc
    13081 tggcagttac cgtggctgaa atccaccacc agatgttcag gtaaatcaaa ctcgtgcagc
    13141 gtatcgcagg ctgcggcgat atcatcggca tgataattcg gttttttgcc gccacgcata
    13201 ataatgtggc catacgggtt gccgctggtc tgatagatgg tcatctgacc atttttgtct
    13261 ggcgagagga acatatggct ggcgcgggct gcgcggatag catccacggc aatccgcgtg
    13321 ttgccatcgg taccattttt aaaacctacc ggacaggaga gtgctgaagc catttcgcgg
    13381 tggatctgac tttcggtagt acgagcgcca atcgcgcccc aactgattaa atcagcaata
    13441 aactgaccgg ttaccatatc gaggaactca gtcgcggtag ggacacccag ctcatttacc
    13501 tgtaaaagta atttgcgcgc cagctccaga ccgtgattta cccgatagct gccgtttaaa
    13561 tccggatcgg agattagccc tttccagccg acaacagttc gtggtttttc aaaataggtg
    13621 cgcattacga tttccagccg tgactggtac tggttgcgca gcgactgcag acgggtggcg
    13681 tactccattg cagcggtgag atcgtggatc gagcaggggc caataatgac caacagtcgc
    13741 ttatcttcac cattcagtat tttttcaatt ctgcggcggg agtcggtgac atgggtggcg
    13801 acgccaggcg ttacgggata ccgtagcgcg agttcggcgg gcgttaccag gctctcaata
    13861 cgcgcagtac ggagttcgtc agttctgttc attacaagtc tcagaaattt gttgcttcta
    13921 cggcaggact accggaaagt gatgcgcatc acgataaacc aatcgcagct gatttcaagt
    13981 gcgaggtgtg tcacacctcg ccgggtggtt agcatgataa caaaaaaaca aaccaatgca
    14041 ctagttctct agtacattcg gcgactaagg cccatgatat cgaggatctt ggtggcaatc
    14101 tcttctaccg aataattggt actgttaatc cacgggatct gatttttacg gtacaaggct
    14161 tccacttccg cgacttccat cctgcactga cgaagcgagg catagcgact gttctcccga
    14221 cgttcctcgc gaatcgccgc cagacgttcc gggtcgatag tcaggccgaa caatttatgc
    14281 tgaagcggtt tgagcgacgc gggtagcacc agattatcca tatcgtcggc aataaagggg
    14341 tagtttgccg cgcggatacc aaattgcatt gccagataca gactggtggg ggttttacca
    14401 cagcgagaaa caccgagcag gatcacctga gcctgatcca gattgcgcaa cgaaatgccg
    14461 tcatcgtggg cgagggtgta atcaatcgcc gcaatgcgcg catcatattt attgagatta
    14521 ttagggttaa ggccatgggt acgatgagca atcggcgttg gatccagttt catctcttgt
    14581 tgtagcgggg caaccagcgc ctgaacgata tcctggcaaa agccttcact ttgcaagatg
    14641 atggcgcgaa tctccggcaa cacgatggag tagaagacca gcgggcgcac gcctgtctgg
    14701 tgataaattg cgtcaatctg atccttcact gcccgtgcac ggctctcatt ttcgacaaac
    14761 ggcagcgtga tgctgctgat agtgacggga aattgtgaca ttactgcgtg tcctaatacc
    14821 tccgcagtta ttgccgtacc atcagaaata taaaaaacgt ggcgatcaac agcattatcc
    14881 attttgttct tcccgtgaag cagacataat tctatgaaag cataaattaa aagcgcacag
    14941 aagcgtagga cgtaatgtct ggtttataaa atgaaccttc attttatttt ttataaaaac
    15001 agcatttcat ttttatggtt tcgtttatac cgatggttta tgtggaaatt gtcgaataga
    15061 gcaggtttgc gcaacgctgg gatcagtctt aaaaagtaaa aaaatatatt tgcttgaacg
    15121 attcaccgtt tttttcatcc ggttaaatat gcaaagataa atgcgcagaa atgtgtttct
    15181 caaaccgttc atttatcaca aaaggattgt tcgatgtcca acaatggctc gtcaccgctg
    15241 gtgctttggt ataaccaact cggcatgaat gatgtagaca gggttggggg caaaaatgcc
    15301 tccctgggtg aaatgattac taatctttcc ggaatgggtg tttccgttcc gaatggtttc
    15361 gccacaaccg ccgacgcgtt taaccagttt ctggaccaaa gcggcgtaaa ccagcgcatt
    15421 tatgaactgc tggataaaac ggatattgac gatgttactc agcttgcgaa agcgggcgcg
    15481 caaatccgcc agtggattat cgacactccc ttccagcctg agctggaaaa cgccatacgc
    15541 gaagcctatg cacagctttc cgccgatgac gaaaacgcct cttttgcggt acgctcctcc
    15601 gccaccgcag aagacatgcc ggacgcttcc tttgccggtc agcaggaaac cttcctcaac
    15661 gttcagggtt ttgacgccgt tctcgtggca gtgaaacatg tatttgcttc tctgtttaac
    15721 gatcgcgcca tctcttatcg tgtgcaccag ggttacgatc accgtggcgt ggcgctctcc
    15781 gccggtgttc agcggatggt gcgctccgac ctcgcatctt ctggcgtgat gttctccatt
    15841 gataccgaat ccggctttga ccaggtggtg tttatcactt ccgcatgggg ccttggggaa
    15901 atggtcgtgc agggtgcggt taacccggat gagttttacg tgcataaacc gacactggcg
    15961 gcgaatcgcc cggctatcgt gcgccgcacc atggggtcga aaaaaatccg catggtttac
    16021 gcgccgaccc aggagcacgg caagcaggtt aaaatcgaag acgtaccgca ggaacagcgt
    16081 gacatcttct cgctgaccaa cgaagaagtg caggaactgg caaaacaggc cgtacaaatt
    16141 gagaaacact acggtcgccc aatggatatt gagtgggcga aagatggcca caccggcaaa
    16201 ctgttcattg tgcaggcgcg tccggaaacc gtgcgctcac gcggtcaggt catggagcgt
    16261 tatacgctgc attcacaggg taagattatc gccgaaggcc gtgctatcgg tcatcgcatc
    16321 ggcgcgggtc cggtgaaagt catccatgac atcagcgaaa tgaaccgcat cgaacctggc
    16381 gacgtgctgg ttactgacat gaccgacccg gactgggaac cgatcatgaa gaaagcatct
    16441 gccatcgtca ccaaccgtgg cggtcgtacc tgtcacgcgg cgatcatcgc tcgtgaactg
    16501 ggcattccgg cggtagtggg ctgtggagat gcaacagaac ggatgaaaga cggtgagaac
    16561 gtcactgttt cttgtgccga aggtgatacc ggttacgtct atgcggagtt gctggaattt
    16621 agcgtgaaaa gctccagcgt agaaacgatg ccggatctgc cgttgaaagt gatgatgaac
    16681 gtcggtaacc cggaccgtgc tttcgacttc gcctgcctgc cgagcgaagg cgtgggcctt
    16741 gcgcgtctgg aatttatcat caaccgtatg attggcgtcc acccacgcgc actgcttgag
    16801 tttgacgatc aggaaccgca gttgcaaaac gaaatccgcg agatgatgaa aggttttgat
    16861 tctccgcgtg aattttacgt tggtcgtctg actgaaggga tcgcgacgct gggtgccgcg
    16921 ttttatccga agcgcgtcat tgtccgtctc tctgatttta aatcgaacga atatgccaac
    16981 ctggtcggtg gtgagcgtta cgagccagat gaagagaacc cgatgctcgg cttccgtggt
    17041 gcgggccgct atgtttccga cagcttccgc gactgcttcg cactggagtg tgaagcagtg
    17101 aaacgtgtgc gcaacgacat ggggctgacc aacgttgaga tcatgatccc gttcgtgcgt
    17161 accgtagatc aggcgaaagc ggtggttgaa gaactggcgc gtcaggggct gaaacgtggc
    17221 gagaacgggc tgaaaatcat catgatgtgt gaaatcccgt ccaacgcctt gctggccgag
    17281 cagttcctcg aatatttcga cggcttctca attggctcaa acgatatgac gcagctggcg
    17341 ctcggtctgg accgtgactc cggcgtggtg tctgaattgt tcgatgagcg caacgatgcg
    17401 gtgaaagcac tgctgtcgat ggctatccgt gccgcgaaga aacagggcaa atatgtcggg
    17461 atttgcggtc agggtccgtc cgaccacgaa gactttgccg catggttgat ggaagagggg
    17521 atcgatagcc tgtctctgaa cccggacacc gtggtgcaaa cctggttaag cctggctgaa
    17581 ctgaagaaat aaaataaatc cccggcggcg ttcagtcgcc ggggttatgt gatccccgaa
    17641 gatgaaactt attcaatctc ttcacagaca tcctgcgtta atcgccgtat aatatctttt
    17701 cttaacaaaa acttttgtat tttatctgag gcagtgcgcg gtagtttttc gattaccacg
    17761 atatgttcag gatatttata ttttgcgacc cgtttacggc taaaaaaagc cgccatctct
    17821 tccagcgata atgaatgatg cggcgctttc agcacgacat aagcgcatga tcgttcacct
    17881 aaacgttcat cgggcattgc aaccacacag gcatcgtgaa ttttaggatg ctgcaataaa
    17941 atatcttcca cttcacggct gctaatattt tcgccgccgc ggacaataat atcttttttg
    18001 cgtccggtaa tttttatata gccagcctca tccatacggc agagatcgcc gctgtaatac
    18061 cagccttctt catccagcgc acgggcggtt aattcaggtt catcaaaata ccccataaac
    18121 acattggggc cacgcgatgc ttcttcacct tcgcaacctg gcggtaaggt cttgcgtgcg
    18181 tcatcgacca ctttaatctc tacacctgcg gcagcgtaac catcggtatg cataaagcgc
    18241 gacaaagaat tatcgagatt caccaccgca tgcggcgaac tttctgtgga accataaaca
    18301 cttaataatt taatgctgcg ctgctggcat tcgcgcgcca cttttttggg gattgtggta
    18361 ccgccgcaaa gaaagaagcg cagcgctgaa aggtctgcgg gttgtttctc tagtaaattc
    18421 aaaagatcat agacaaacgg cgttgcgcct agcatacagg tgcaacgctg ctgctcaagc
    18481 agcgcgagac acgcatcagg agtgaaaata tctaacaaca cgctgcgagc gccaattaag
    18541 aatggtgccg ttacgccatg cagaaagccc gttgcgtgac caagtggcgc aggcatcata
    18601 aagacatcca gccaggtcag attcagccgc gcgcaataag cccgctcact ggcgagaata
    18661 ttgttatgcg ttagcatcac gccctttggc agaccctcgg ttccggaggt aaacagcacc
    18721 gcagctaatt catcgccgtg ggtcgttatc gccgtggtca gtgggatatt gtcggcgata
    18781 atctgactta atgagaggga agaggtggcg ggagccagtt tgtccacgcc gacaatttgt
    18841 tgtagttgtg gaagctgatt ttgcagcggc aggattaaat ctaccggacg cgtttgttta
    18901 aacaacgtcg gtgcaaagaa catttttgcc tgacacttat tgagcaccca caccagttct
    18961 gcttcccgcc aggaaggcaa cagcggcacg gaaaccgcac cgattttcag gcaggcaaga
    19021 tagataacgg taaattcaca ccagccaggc agttgaaatg cgatgcgatc gcctgattca
    19081 atacccttcg ctaacatcca gtttgccaga cagctcgcgg cgtgatcgag cgcgctatag
    19141 gtgtacgatg caccatgatt atcgaccacg gcaattttgt ctggcatcgc acgagcggtc
    19201 tgctgccagt aatcggccag tgaagcatcg ccccatagcc cttgctgacg atacgccgca
    19261 cgacgttgtt cgttaaacgt taatgtcact ttcattttgg actctcgaaa cagaacatca
    19321 ggcccgagtt gcgggcctgt gggatgcatg attagccgta gcgaaaatca ataccgaagg
    19381 tgcctgcggg atattgccac tgttcgagaa tagtgttacc gcacagcacc cgacaggtgc
    19441 cgcactccag acaaccggcg ggatcaaaat gaatgtttcc tacgtcatcc tgcttataaa
    19501 gtccggcagg gcaggctttc attaatttac ggaattcatt gatatcggga ttttccgtca
    19561 gaatgatatg cgggtggccc tcatcaacat ggaatttatt gacgcctaat ttgatgtcga
    19621 cgttaaccgt agcgttctgg ctcatagcgc ggttgctccc ttaatgccat ctttcagcaa
    19681 gttgatcagc ccaattttct tcgcgtgtcc catgatcatt ttgcgtaccg gctggtttgg
    19741 tttgccgtca atggtgaaca tctcgttcat gatgtcggct accattcgtg ggtattggct
    19801 aaacaggcgc gggttttcca tcagcgccgg gatcttgcga aaatgctgca tatcacgcat
    19861 gacgcagctt tgttccagct cacgtttgta ttgcgccaga ctgttggcgg agaaatccgc
    19921 gcgttctttg gcggcgatca ccgttgtggc ggcagcctga gccgatgcaa tggctaaatc
    19981 catgccgcgg accgtaaaac ccaaattcag gcagaagcct gcggcgtcac caacgatcat
    20041 cacgccctcg ttaaccagtt gcggcaccat tgccagaccg ccttccggca ccatatgcgc
    20101 ggaatattca agcagtttgc cgccgctaat cagcgggcga atggcggggt gttgtttaaa
    20161 atcttccagc atttgcggca cgcttttttg cgcatgggcg atatcaccca atccacaaac
    20221 cagccccaag gatatggaat ccttgttggt atagagaaat cccccgccca tcaggccgtc
    20281 agaaggggaa ccggcaaaca gccaggcggc accttcctcg cccgtaatat taaagcgatc
    20341 gttgatctgt tctggtgtga ggccaataac ctctttaaca ccaacagcgt aatgatgcgg
    20401 atcggaagcg ggaaccattc ccagcgagcg gccaagcatc gagttaacgc catcagccag
    20461 aatcaccaca ttcgcttcga gaatatcatc cccagcctgc acgccagtga ccttgtttcc
    20521 ttcacgaacc agcgcgtcga cgcgaacgcc tgggataaac tgcgcgcccg cctgctcggc
    20581 ttgttccatc aaccacgggt ccagacgatt acgcaatacg gtataagatg cgtgttgagg
    20641 aacatctggt tgctcgcggt gaaaatcgag ggtaacggcg ctttcttcgg ttaagaagga
    20701 gattttctcg cgtgtgacct tgcgttctac cggcgctgat actgcaaagc ctggaatgat
    20761 tgcttcaagt gtgtgggcat aaagacgccc gccggtcatg tttttacatc cggcactgtc
    20821 gccgcgttct atcaccagga catccagccc ggctcgtgcc atgaccagtg cggcaacgct
    20881 accagcaacg cccgcaccga ccacaatggc atcaaatttg tcatccgaca tgcgttatgc
    20941 tcccttcctg ctgcgtttac agcgggagat gtggttgaaa ggggaattag cggctcaact
    21001 ggctaatcag ggcagggacg actttgtaga tatcgcccac cagaccgtag tcggcatagt
    21061 tgaagattgg cgcattttta tctttattga tggcgacaat cacttttacg ccgttgccgc
    21121 caaccatatg ttggatctgc ccggagatcc ccagcgtcag gtagagatcg gatttcagca
    21181 acacgccgga gacaccgata taacgttcac gctccatcca gttctcgcct tcggcaattg
    21241 gacgtgaaca gccgacttca gcattcagca ccgccgccag ttcgtggacc attttcaggt
    21301 catcctgcgc cgccagacca cgaccgacgc caaccacacg ttttgctttg ctcaggtcca
    21361 cgctgcttgc ggctttcgca cggcgttcgc gacagagaat ttcatgacgc ggagcaacat
    21421 aaggtaccgt ttctgtcggg cactgatgag aggtatcact ggtgcacggt tcctgaacac
    21481 cgggtgcaag ggtaatgatc gccagcgggc tgttgatctt ttcctgagcg aacgctaacc
    21541 cgccatacat ccggtgttcg gcgcaaatgt gaccatcgac aatatccacc gccgtggcat
    21601 cgttcaccag cgccgcattc agttgcacac ttaaccgtgc tgccagagct ttaccacgtt
    21661 tcgtcgcggc caacagcaac atagcggggt gtttatcttt cagcagggca gcaatgcttt
    21721 cggcgtaatt ttcagtgcgt tgcagcgcgt cgttttgctc aagaacataa atacattttg
    21781 gaccataagg cataactgcc tgcgcctggt cggtattttg tacaatggca tacacctgtt
    21841 ggccccattg ctgagcgccg ccaaacagtt cagcataacg ttcaggatta tcgctaaaga
    21901 cccagacgct gtttaattga ctcatcgttt cttctccgta ggcttcagtt cagggctttc
    21961 ttcagatgtt ccgccagctc ggcaatggcc tccggcgaat cgttatcgag aatgatgtgc
    22021 ttacgttctg tttgcggcgg tacgcgaatg ccgaccagtt cagcaagtgg cgcgctctgg
    22081 ctccagtcaa tatcacttgc ctgccactga tttaccggtt ttttacccgc gccgagaatg
    22141 gctttcatcg aaggaatgcg tggcacgtta atatcggagg tgacgcagag cacggctgga
    22201 acagagagtt caataacttc aacatcatct tcaagcgtgc gttcaatcac cagtgtattg
    22261 ccctgacgct gaatagcact cactgcatta atcaccggaa gttgcagaat ttctccgacc
    22321 agcaagccaa cctgctgggc ataaaggtcg ccggaacctt caccaaagat cagtaaatcg
    22381 aagccgatct tttcaattgc tgccgccagc gcttttgcgg tatcgagagg cagtgcatgt
    22441 tcaagttgcg catcctgcac caaatacagg ctgtgcggcc cgcgggatag cacgtctttg
    22501 cgcactttcg agttctgcaa caatgagccg ccaacggtca gcgcggctat ctcatcgtca
    22561 tctgttgcga gctggcttgc agcttcaatg gcattgagat cgaactggct gattttggcg
    22621 tcggcattgt cgaaattcag ggtgtattct ggagtgacaa caatgtcctg ttcttcaggc
    22681 accagcttaa agcaggttat tattttcatg atttctcctg tgagtcgcat acggaaccat
    22741 ctccgatgga aggtaaaaat ggcctgaaaa tagtgt
//
LOCUS       DAJTRH010000060        22458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTRH010000060 DAJTRH010000000
VERSION     DAJTRH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            83..2683
                     /locus_tag="OO339_002526"
     CDS             83..2683
                     /locus_tag="OO339_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5927118.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2674..3657
                     /locus_tag="OO339_002527"
     CDS             2674..3657
                     /locus_tag="OO339_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5927119.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3755..4295
                     /locus_tag="OO339_002528"
                     /pseudo
     CDS             3755..4295
                     /locus_tag="OO339_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4303..4818
                     /locus_tag="OO339_002529"
     CDS             4303..4818
                     /locus_tag="OO339_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5927120.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4784..5521
                     /locus_tag="OO339_002530"
     CDS             4784..5521
                     /locus_tag="OO339_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5927121.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5632..6642
                     /gene="pyrD"
                     /locus_tag="OO339_002531"
     CDS             5632..6642
                     /gene="pyrD"
                     /locus_tag="OO339_002531"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5927122.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6816..7358
                     /gene="zapC"
                     /locus_tag="OO339_002532"
     CDS             6816..7358
                     /gene="zapC"
                     /locus_tag="OO339_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5927123.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7355..8464)
                     /gene="ycbX"
                     /locus_tag="OO339_002533"
     CDS             complement(7355..8464)
                     /gene="ycbX"
                     /locus_tag="OO339_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5927124.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8708..10816
                     /gene="rlmKL"
                     /locus_tag="OO339_002534"
     CDS             8708..10816
                     /gene="rlmKL"
                     /locus_tag="OO339_002534"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5927125.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10828..12735
                     /locus_tag="OO339_002535"
     CDS             10828..12735
                     /locus_tag="OO339_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5927126.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12865..14118
                     /gene="pqiA"
                     /locus_tag="OO339_002536"
     CDS             12865..14118
                     /gene="pqiA"
                     /locus_tag="OO339_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5927127.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14123..15763
                     /gene="pqiB"
                     /locus_tag="OO339_002537"
     CDS             14123..15763
                     /gene="pqiB"
                     /locus_tag="OO339_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5927128.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15760..16323
                     /gene="pqiC"
                     /locus_tag="OO339_002538"
     CDS             15760..16323
                     /gene="pqiC"
                     /locus_tag="OO339_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5927129.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16579..16746
                     /gene="rmf"
                     /locus_tag="OO339_002539"
     CDS             16579..16746
                     /gene="rmf"
                     /locus_tag="OO339_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5927130.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16816..17334)
                     /gene="fabA"
                     /locus_tag="OO339_002540"
     CDS             complement(16816..17334)
                     /gene="fabA"
                     /locus_tag="OO339_002540"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5927131.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17403..19163)
                     /locus_tag="OO339_002541"
     CDS             complement(17403..19163)
                     /locus_tag="OO339_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5927132.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19348..19800
                     /gene="matP"
                     /locus_tag="OO339_002542"
     CDS             19348..19800
                     /gene="matP"
                     /locus_tag="OO339_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5927133.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19876..20922)
                     /gene="ompA"
                     /locus_tag="OO339_002543"
     CDS             complement(19876..20922)
                     /gene="ompA"
                     /locus_tag="OO339_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5927134.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21279..21788)
                     /gene="sulA"
                     /locus_tag="OO339_002544"
     CDS             complement(21279..21788)
                     /gene="sulA"
                     /locus_tag="OO339_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5927135.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22007..22417
                     /gene="sxy"
                     /locus_tag="OO339_002545"
                     /pseudo
     CDS             22007..22417
                     /gene="sxy"
                     /locus_tag="OO339_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
BASE COUNT         5736 a         5271 c         5881 g         5570 t
ORIGIN      
        1 gtatcgaacc atcaatgact acggggcgga aacgccgcaa ctcaactgta aatcgtaagc
       61 cgtcttcagt taagagagcg agatgtatag aactcatcga caacacagcc tgttaagctc
      121 tggtggagtg ccatcgttta ttggtgggct ggtggtgttt gtgtcggcag cgttcaatgc
      181 acaagctgaa acctggttcg atcctgcttt tttcaaagat gatccctcaa tggtggccga
      241 tttgtctcgt ttcgaaaaag gacaaaaaat aacgccaggg gtttatcgtg tcgatattgt
      301 tctgaatcag acaattgtag atacgcgcaa cgtcaatttt gttgagataa cgccagagaa
      361 ggggattgcc gcctgtttga cgactgaaag cctggatgca atgggtgtta atactgatgc
      421 gtttccggct tttaaacaac tggacaaaca agtgtgtgtg ccattggcgg agattattcc
      481 ggatgccagc gtaacgttta atgtgaataa actccgtctg gaaatttcag taccgcaaat
      541 cgccatcaaa agtaatgccc gtggttatgt cccccctgaa cgttgggatg aagggatcaa
      601 cgcgctatta ctgggatatt cgtttagcgg ggctaacagt attcatagca gcgcagacag
      661 tgattctggc gacagctatt ttctgaattt aaacagtggc gttaatttag gcccatggag
      721 attacgcaac aattcaacat ggagtcgcag tagtggccaa accgcagaat ggaagaatct
      781 cagcagctat ttgcagcggg cggttattcc actaaaaggc gaactgaccg taggtgatga
      841 ttatactgca ggcgattttt tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga
      901 tgacaacatg ctgccagaca gcctgaaagg gtttgcgcct gtggtgcgtg gtatcgccaa
      961 aagcaatgcc cagataacga ttaagcaaaa tggttacacc atttaccaaa cttatgtatc
     1021 gcctggtgct tttgaaatta gtgatctcta ttccacgtcg tcgagcggtg atttgttagt
     1081 tgaaatcaaa gaagcggacg gcagcgtcaa tagctacagc gtaccgtttt ccagcgtgcc
     1141 attactccag cgtcaggggc gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa
     1201 tagtaatgaa cagcaggaga gcaaatttgc ccagaccacg ttgcagtggg gcggaccgtg
     1261 gggaacgaca tggtatggtg gtggacaata tgctgaatat taccgtgccg ccatgtttgg
     1321 tctgggattt aaccttggcg atttcggagc aatttcgttc gatgcgaccc aggcgaagag
     1381 tacgctggca gaccaaagcg aacataaagg tcagtcatat cgttttctgt atgccaaaac
     1441 gctcaaccaa ttgggcacta attttcaatt gatgggctat cgctattcga cgtcgggttt
     1501 ctacaccctt tccgacacca tgtataaaca tatggatggc tacgaattta atgacggtga
     1561 tgatgaagat acgccgatgt ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa
     1621 actgcaggtc aatatctccc agcaattagg cgagtacggt tcgttttatt taagtggtag
     1681 ccagcaaact tactggcata ccgatcaaca ggatcggcta ttacagtttg gctacaacac
     1741 gcaaattaaa gatctctcgc tgggggtttc ctggaactac agtaagtccc gtggtcaacc
     1801 tgatgctgat caggtgtttg cactaaattt ttccctgccg ctcaatctgt tgctccccag
     1861 aagtaatgat agctatacca ggaaaaaaaa ttacgcctgg atgacctcta acaccagtat
     1921 cgataacgaa gggcacatta cacaaaacct gggtttaacg gagacactac tcgatgacgg
     1981 taacctgagc tacagcgtgc aacagggata taacagcgag gggaaaacgg ctaatggtag
     2041 cgccagcatg gactacaaag gggcgtttgc agatgcccga gtgggctaca actacagcga
     2101 taacggcagt caacaacaac tgaactacgc tctttcaggc agtttagttg cccattcaca
     2161 gggcattacc ctggggcaat cgctggggga aactaacgtg ctgattgcag caccaggcgc
     2221 agagaatact cgtgtggcga acagcaccgg gctgaaaact gactggcgcg gatataccgt
     2281 tgtcccctat gccacttctt atcgggaaaa tcgaatcgca cttgatgcgg catcgttaaa
     2341 acgtaacgtg gatcttgaaa atgcagtagt caacgtggtt cccaccaaag gggcgttggt
     2401 tctggcggag ttcaatgccc atgcgggagc aagggtatta atgaaaacat caaagcaggg
     2461 tataccgctg cgttttggcg cgatagcgac gctggacggc atacagacta atagcggcat
     2521 tattgatgat gatggctcgc tctatatgtc cggtttgccc gctcagggag cgataactgt
     2581 acgctggggc gaagctcccg atcaaatttg tcatatcagt taccagctta ccgaacaaca
     2641 aattaactct gcgattacgc gaatggatgc catatgcaga taatctttgg agaaaaatgc
     2701 gtgttattac tacgactatt ttttgccgcc gtcttaatgc tatggtgcgc tcaaactgct
     2761 gcttatatcg ggcagtgtca taccactcag gggaatccgt atattggcgt caattttggc
     2821 gttaaaactc tggatgaaga agaaaatacg gctggagtag ttaaagacaa attttatcag
     2881 tggaacgaat cgaatgatta ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt
     2941 ggccgatggg cattcgccgc ggattcaccg ttagtctatt taggcgacaa ctggtacaaa
     3001 attaatgact atcttgccgc caaagtttta ttgcaggtta aaggcagttc tcctactgcg
     3061 gttcctttcg aaaacgtggg cacaggggca gatacacgat ggcatatttg cgatcccggc
     3121 ggtcaacgtt taggtggcca gggggctagc ggtaatagcg gtagcttttc cctgaaaata
     3181 ttgcagccgt tcgttggttc ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa
     3241 tgctacaaca tacccgcagg tgattcctgc acgactactg gtacaccggt tttagtgtat
     3301 tacctgtctg gtacgatcaa ttcacttggc tcatgttccg tcaatgccgg agaaacaatc
     3361 gaggttgatc tgggcgacgt atttgcggct aactttcgtg ttgtagggca taagcctctt
     3421 ggggccagaa cggcagaact tgcaattccg gtcaggtgta acacgggaaa cgcagggtta
     3481 gttaacgtca atctgagtct gacggcaacc tcagacccca gctatcccca ggcgattaag
     3541 acgtcacgtc ctggcgtggg cgtggtggtg accgatagcc agcacaacat tatttcccct
     3601 gctggtggga cactaccgct ctctattcct gatgatgcag acagtatccg cgaatgaatg
     3661 tctatccagt cagcacgaca agcgtaccac cagaaaccgg gcgatttgaa gccacggcaa
     3721 cggtgagaat aaattttgat taacgcgtga acgtatgaag aaaaaaacga tatatcagtg
     3781 cgttattttg ttctttagcc ttcttaacat ccatgtcggg atggctgggc ctgaacaagt
     3841 cagtatgcat atttatggga atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc
     3901 attacaaaat attcatattg gtgattttaa tatcagtgat tttcaggccg cgaataccgt
     3961 aagcactgct gctgatttga atattgatat caccggttgt gccgctggta ttactggcgc
     4021 ggacgtcctt tttagcggcg aggctgacac ccttgcgccg acactgctca aactaactga
     4081 cacaggcgga agcggtggta tggcaacggg gattgccgtg cattcttgat gcgcaaagtc
     4141 agcaagaaat cccgctcaat caggtccagc ctcttacgcc cttaaaagcc ggggataaca
     4201 cactcaaata tcaacttcgt tataagtcca caaaggcggg agcaacgggc ggtaatgcga
     4261 cggcggttct ctattttgat ctggtttacc agtgaaggag tgatgttgaa acggataatc
     4321 tggattctgt tcttactggg attaacgggg ggctgtgagc tatttgccca tgatggcacg
     4381 gtcaacatta gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa
     4441 atcaatgtgc agttaggcga tgtctcgctg acacgttttt ttcatgggaa ttatggcccg
     4501 gagaaatctt tcatcattaa tttgcaggat tgcggaacag atgtcagcac ggttgatgtc
     4561 accttttcag gaacccccga cggagtgcag agcgaaatgc tgtccattga aagtggtacg
     4621 gatgcagcca gtgggctggc aattgcgatt ctggatgatg cgaagatatt gattccgctt
     4681 aaccaggcga gtaaagacta cagcctacat agcggtaagg tgccattaac tttttatgcg
     4741 caattgcgac ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt
     4801 gtacttcatt atgactaata cgtggaacag attagcgctc ttgattttcg ccgttttatc
     4861 gctgctggtg gcgggtgaat tgcaggcagg agtggtggtc ggcggaacgc gatttatctt
     4921 tccggcagac agagaatcga tatctatttt actgactaat accagtcagg aatcttggct
     4981 tattaacagc aaaatcaatc gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc
     5041 agcactatta ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt
     5101 gcgcttgctg agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt
     5161 aagcattgcc agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg
     5221 ctcggtattt aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta
     5281 tcaacaatta cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta
     5341 ctacattaac ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagtcgt
     5401 gccgcctaaa agccagcgtc agacaaactg gtgtcaggta atagcaccgt gtcatgtcgc
     5461 ctggcggacg ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg
     5521 aaacaggttc tgaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga
     5581 actcgcacat aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac
     5641 cccttcgttc gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt
     5701 cagcaattac gccgcattac cggaacaccg tttgaagcac tggtgcggca gaaagtgcct
     5761 gcgaaacctg ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt
     5821 cttgataaag acggggagtg cattgatgcg ttaggcgcga tgggatttgg atcgatcgag
     5881 atcggtaccg tcacgccacg tccacagcct ggtaatgaca agccgcgtct ctttcgtctg
     5941 gtagatgccg aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc
     6001 gtagagaacg taaaaaaggc ccattatgac ggcgtcttgg gtattaatat cggcaaaaat
     6061 aaagatacgc cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat
     6121 gcctatgcgg gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg
     6181 ctgcaatatg gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat
     6241 ttgcaagcga tgcaccataa atatgtgccg atcgcagtaa agatcgcgcc ggatctttct
     6301 gaagacgaat tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt
     6361 gcaaccaata ccactctcga tcgttctctg gttcagggaa tgaaaaattg cgatcaaacc
     6421 ggtggcttaa gtggtcgtcc gcttcagtta aaaagcacag aaattattcg ccgcttgtca
     6481 ctagaattaa acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct
     6541 gcgcgtgaaa agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt
     6601 aaaggtccgc cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct
     6661 ctttataaga taatcagggc tttattttca gacctggttg ttttatattc atcgttgttg
     6721 cttatttaga cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg
     6781 tgttgctgcg gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact
     6841 ggcgttggta ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc
     6901 tatttcgctc acgttttgcg cgcaaaatgt tgactccaga tgccttttct cccgcaggct
     6961 tttgtgttga tgatgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt
     7021 tatctaaaga ccaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgttatctca
     7081 aaccgcaaat gccgaaaagc tggcattttg tctcccatgg tgaaatgtgg gtgcccatgc
     7141 cgggcgatgc ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg
     7201 ttgaatctgg cgaaaatgcc gcgctatgcc tgctggcaca accctgcgtt gttattgcgg
     7261 gtcgcgcgat gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg
     7321 tgaatgttga cagcttcagc ctcgaacagg cagtctaacg cgtcagctta agggcagtct
     7381 tcggtacaca gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg
     7441 gtgtgacttc gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag
     7501 aataagggat acgaattccc tgattttcta attgctccag caacacttgt tggttatttc
     7561 cacgaaatgc ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt
     7621 tggcggtatc atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt
     7681 ccacctcatc gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat
     7741 cgacatcgcc gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg
     7801 ccggatgttt ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt
     7861 taaccacatc aaacaccaca tcaccaatgc gaatcacttt ccagctatct tcttcccagg
     7921 ctgacgcgcc ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac
     7981 aacgttgttg gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag
     8041 caaatgacag aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc
     8101 agcgtaattg cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg
     8161 caattcgcgc ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat
     8221 cagcaaaacg aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca
     8281 caggtgaagg agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt
     8341 caggttccgt gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat
     8401 gtgtaagacc aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg
     8461 ccacggtgcg gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta
     8521 gctataatgc gcaacaattt tcttagcgcc tgtcccgtta ggctatttta tttgtcagtt
     8581 tggccctggg ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct
     8641 ctccatgtcc agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag
     8701 tgacgatatg aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac
     8761 tgaactggaa aatctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa
     8821 gggcgacaca cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat
     8881 gttgccgctg ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc
     8941 gatcaactgg acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt
     9001 gaatgacacc atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga
     9061 tgctttcaca cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg
     9121 cgttaacgtc tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg
     9181 tttacatctg cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc
     9241 agccgctatt gtgatgcgat ccggctggca gtcaggaaca ccgctgctcg atccgatgtg
     9301 tggttccggt acgttgctga ttgaagcggc gatgctggcg accgatcgcg caccaggctt
     9361 gcaccgtggg cgttggggct ttagcggctg ggcgcagcat gatgaagcta tctggcagga
     9421 agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggccta gctgagtata gctctcattt
     9481 ttacggttcg gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc
     9541 ggggattggt gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct
     9601 gccgaaaggg ccgtacggta cagtgttgag caacccgcca tacggtgaac gtctggacag
     9661 cgaaccggcg ctgattgcgc tgcatagtct gctgggccga atcatgaaaa accagttcgg
     9721 tggctggaat ctctctttgt ttagtgcctc gccggatctg ctaagctgcc tgcagctgcg
     9781 tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca
     9841 cgttgccgaa agcacaccag acagtaaacc ggcaatggta gcggaagatt acgccaaccg
     9901 cctgcgtaag aacctcaaaa aattcgagaa gtgggcacgc caggaaggga ttgaatgtta
     9961 ccgcctgtat gacgccgatc tgccggaata taacgttgcc gttgaccgtt atgccgactg
    10021 ggtggtggtg caggagtatg cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg
    10081 tctgttcgat attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt
    10141 gctgaaaacc cgtgaacgcc ataagggcaa aaaccagtac cagaaactgg gcgagaaggg
    10201 cgagtttctt gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct
    10261 cgatactggt ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa
    10321 aggcaaagat ttcctcaacc tgttttctta taccggcagc gccaccgtgc atgcgggatt
    10381 aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg aatgggcaga
    10441 acgcaacctg cgtctgaatg gcctgactag gcgtgcgcat cgcctgattc aggccgattg
    10501 cctggcgtgg ctgcgtgagg caaatgaaca gttcgatctg atctttatcg atccgccaac
    10561 cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct
    10621 gatgaaagat ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa
    10681 acgtggcttc cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat
    10741 tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct
    10801 gattaccgca gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg
    10861 tcgttcagcg acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt
    10921 gtttgtctgg tgggccgcaa cggcgcagga aaatcgacgt taatgaaaat cctcaaccgt
    10981 gaacaagggc tggatgacgg tcgcattatt tatgagcaag atttgattgt agctcgtctg
    11041 caacaggatc cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt
    11101 gaagaacaag cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac
    11161 ccgagcgaga aaaatctcaa cgaactggcg aaggttcagg aacagctgga tcaccacaac
    11221 ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaac
    11281 gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg
    11341 gtgagcaatc cgcgcgtatt gctgctggac gaaccgacta accatctgga tatcgaaacc
    11401 atcgactggc tggaggggtt ccttaaaacc ttcaatggga cgattatttt catctcccac
    11461 gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg
    11521 gtgacctatc cagggaatta cgaccagtac ctgcaggaaa aagaagaagc cctgcgcgtg
    11581 gaagaactac aaaatgccga gttcgatctc aaactggcgc aggaagaagt gtggattcgc
    11641 caggggatca aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcgatg
    11701 cgtcgcgaac gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag
    11761 gccagccgct ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttaacggt
    11821 aagcaactgg tgaaagattt ttctgcccag gttctacgtg gcgacaaaat tgccctgatt
    11881 ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactga tgctcggtca gcttcaagcg
    11941 gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc
    12001 gcggaactgg atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg
    12061 atggttaatg gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg
    12121 aaacgggcga tgacgccagt acgtgcgttt tctggcggtg agcggaaccg cttgctgctg
    12181 gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt
    12241 gatgtcgaaa cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg
    12301 ctggttagcc acgatcgtca gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa
    12361 ggcggcggta aaattggtcg ttatgttggc ggttatcatg atgcccgtgg tcagcaagaa
    12421 caatatgtgg cactcaaaca gcctgcggtg aaaaaaagcg aagaagccgc cgcgccaaaa
    12481 gcagaaactg taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag
    12541 cagctaccgc aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaagtg
    12601 gcggatgctt ccttcttcag tcagccgcat gagcagacgc aaaaagtgct tgctgatatg
    12661 gctgctgcag agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta
    12721 aaaaatggtg gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa
    12781 ttgcctgatg aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa
    12841 atagcgcatc attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg
    12901 tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca
    12961 tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc
    13021 gcctatgcgt tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat
    13081 atgaacgttg caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt
    13141 tctgaggact acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg
    13201 ttttgtctga taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa
    13261 gagcaactgg cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc
    13321 gcgggtgtgc tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc
    13381 agctttctcc cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat
    13441 cgtcgctggt tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca
    13501 ggcgtcacgg ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct
    13561 gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc
    13621 ctgcagtgga cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt
    13681 ttgcccatca tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg
    13741 gtcattctgt tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt
    13801 attatggtgc caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg
    13861 catggcaagc gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc
    13921 cgctggtcga tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga
    13981 ggtttaatga gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg
    14041 acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca
    14101 gagcatgagg agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt
    14161 gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt
    14221 tcttttttat cattacagcc atcagggacc ggaagtgacc ctgatcaccg cgaatgcgga
    14281 aggaattgaa ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga
    14341 aagcgccaca ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg
    14401 tatggaaaaa ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg
    14461 cgaagggatt agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg
    14521 cgcgaaaggc agcaaaatgg ataaatacga tttgctggac tcaccaccgt tggccccgcc
    14581 tgatgcgaaa ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg
    14641 agatccggtg ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac
    14701 gcaaaaacgc aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac
    14761 cagcaatgtt cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat
    14821 gcgtgtggag atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc
    14881 ggaaggtctg gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga
    14941 tcagaagagc attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa
    15001 agattcggta cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg
    15061 taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta
    15121 ccgtattccg gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa
    15181 tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa
    15241 aaccggaaac ctggtcactg gtgcactgta tgttgatctc gatttctatc caaatacgcc
    15301 tgcaataacc ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg
    15361 cttggcgcaa atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt
    15421 gaatccgatg attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa
    15481 cctgcaaacg acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt
    15541 gccgacggat atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc
    15601 tggctccgca gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg
    15661 agaactgcaa ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc
    15721 gaaggacaaa aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg
    15781 attgcagcac tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag
    15841 ttacctgtgg tgcagagcgg tacacaaagt accgctagcc agggcaatcg tctgttatgg
    15901 gtagagcagg tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt
    15961 gatgtgaagt atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacagttg
    16021 cgcaacaccc tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag
    16081 cctctgggaa gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat
    16141 gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa
    16201 cgtccgttcc gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagtg
    16261 ctggccggtg tctggagtca ggaagccgct tctattgcac aagagataaa gcgtctacct
    16321 taattataaa gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg
    16381 tttctaacgt gcggaaaaat ttgttcctct tcacattttt tgtacaaccg acatgcccgt
    16441 gtagctcaca aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg
    16501 gtattgctta actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca
    16561 gtatgaggga aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa
    16621 cgtggttatc aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg
    16681 agtcaaaggt cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg
    16741 gcctgattct gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac
    16801 tctgtctgat gataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct
    16861 ggcggtatag atcagacgac aatcaaccag cacttcgcca tctgccagac ccataatcag
    16921 acgacgatta acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag
    16981 tacctgacca gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc
    17041 cagccagccg aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg
    17101 cataaccgga tcgccaatga agtggcatcc gaagaaccac agatccggat tgatatccag
    17161 ttctgcttca acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg
    17221 gtctatcatc agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc
    17281 gtgaccagag gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc
    17341 tgtaagcctt attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc
    17401 gatcagttcg gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct
    17461 tcctgttgcg atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct
    17521 tcgccatccc acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc
    17581 cagatggtga atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg
    17641 cgaacgttag cagtggggat aatgacacct tgtttccccg ttaactcacg ttgctggcaa
    17701 atagcaaaga agccttcgat tttctcattt aaaccaccga ccggctggac gcggccgaac
    17761 tgatcgactg aacctgtgat agcgatactc tgattcactg gcacatcagc gagggcgctt
    17821 atcagggcgc agagttcagc catcgaggca ctatcgccat ccacttcact gtatgactgc
    17881 tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg
    17941 aacgcctgca tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt
    18001 tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg
    18061 ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg
    18121 cgttcgcctt cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca
    18181 gcgaggaaac cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag
    18241 aaggtgtcgc catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg
    18301 ctaagcggaa gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc
    18361 caagcatccg ctcccggggc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga
    18421 caccactggc ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa
    18481 atagcctgct ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg
    18541 cctaccagaa tgactttcag cttcaatggc attgaaggca cagagacggg gagagggcgc
    18601 gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc
    18661 cacagcagag gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc
    18721 tgatgcacca gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag
    18781 agttgctccg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca
    18841 tctgcactca ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgatcgccc
    18901 accagttggc ctgcatcgct ttgtagcgta cgcgcggcat cggcaataag attcagatac
    18961 tcagactctt ccggggcctt agccagcata aaagaggagg atgctcgcgt atgcagcaac
    19021 tgctccagcg caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca
    19081 atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc
    19141 catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt
    19201 ataccgtaac cggcactaca gcacacgtag aaagcaccga caatactcct ggctgggcgt
    19261 taaagctcac aggatggaga ttcttttctt cactggccta aaaagctgat attctgtaaa
    19321 gagttacacg gtaacattga gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc
    19381 ggttggaaat ggaagtatct ggtgaagaag catcgcgaag gggagttaat tacccgttac
    19441 atagaagcca gtgccgccca ggaagccgtc gatgtgttgc tctcgctcga aaatgaaccc
    19501 gtgctggtaa atggctggat tgacaagcat atgaatccgg aactggtcaa tcgcatgaag
    19561 cagaccattc gggccagacg gaagcgccat tttaatgcgg aacaccagca tacgcgcaaa
    19621 aaatcgatcg atctggaatt tattgtctgg caacgtctgg ctggtcttgc acagcgtcgc
    19681 ggtaaaacgc tttctgaaac gattgttcag ctgattgaag atgcggaaaa caaagagaaa
    19741 tacgcgaata aaatgtcttc tcttaagcag gatctgcagg cattgctggg taaggaataa
    19801 ctgacgaaag tcagttcaat ttactaaagg caaaaaaaac cccgcagcag cggggttttt
    19861 ctaccagacg ataacttaag cctgcggctg agttacaacg tctttgatac ctttaacttc
    19921 gatctctacg cgacgatccg gagccaagca gtcgatcagt gcagcacgct gtttcacgtt
    19981 gtcacaggtg ttgccagtaa ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc
    20041 tgccgggata cctttggaga tcaggtaatc aacaacagac tgagcacggc gctcggacag
    20101 accctggttg taagcgtcag aaccgatgcg gtcagtgtaa cccagaacaa ctacggaacc
    20161 gtctttcgga tctaggttgc tcagctggct gtacagctga tccagagcag cctgaccttc
    20221 cggtttcagg gttgctttgt tgaagttgaa cagaacgtca gacttcagag tgaagtgctt
    20281 ggtctgtact tccggtgccg gagctggagc aactaccgga gctgcttcgc cctgaccgaa
    20341 acggtaggaa acacccaggc tcagcagacc gttgtccgga cgagtaccga tggtgtttgc
    20401 gtcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc
    20461 gtactcaaca ccacctgcga aaaccggaga aacgccagtg tcgtggtctt taaaggatgc
    20521 gccaccaggt acgttagcct tggtgtctgc acgccatacc ataccaccca gacgagtgta
    20581 gatgtccaga tcgtcagtga ttgggtaacc cagtttagcg gtcagctgaa cgccctgagc
    20641 tttgtatgcg ccgttgatgt tgtcgccttt gtacggcata cgacctaacc agtcgtaacc
    20701 catttcaaaa ccaacatacg ggttaacctg gtaaccacca aaagcacctg cacccagttg
    20761 gttttcgtgg gtcggaccat tgttaggaat aaaaccagtg tcatggtact gggaccagcc
    20821 cagtttagca ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa
    20881 accagccagt gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc
    20941 atccaaaata cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg
    21001 atctgctcaa tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt
    21061 aaagtctaca acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa
    21121 aagtcttgta taaggtatgt ttaatctttt tgtcagcgac aatttacaga aaaaaaacgc
    21181 ggaaaccgct tcagacaagc cttcgcaagg aaaattagtc acgactgaaa gcattggctg
    21241 ggcgacaaaa aaagttctag gattaatcct aaatttactt aatgatacaa attagagtga
    21301 atttttagcc cggaaagttg tctcgttgtg tgagaggatg cgcttaccgg acgcataata
    21361 aaccccatag cgttaccttc atttgccgca tcaacaagtt cagcatgctc ttcttcagtc
    21421 aaatcatctg tcaaccaacc gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc
    21481 attgactcca ctgtgtggca aggggagagc tggctaatct gcattacttt cgttaagggt
    21541 agcccagatg cctgaaccca ttcccgactc agtttttgtt gcggtgttaa ccagagttgc
    21601 cagcgcgatt gctgaccgag ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg
    21661 ggctgatctt cgcgatagac aacttcactg ataagcccgg ctgtagtgtt ttccgtagag
    21721 acacgcgcaa ttttacttgc tgcggatgag aacgacgaag aacgatgtgc atagcctgaa
    21781 gtgtacataa tcaatccagc ccctgtgagt tactgtatgg atgtgcagta catccagtga
    21841 caacaaagat caaccctatt ttcggaaaga gcctcgcaaa ttttgtcgtt ggtgacggga
    21901 aaacataaat taatcttgcc ccttaagaat aagttgccta ttttcgtagt taacggatcc
    21961 gttaatgtga atcattcttt tatgttatga ttttaaaagg aattttatga aaagcctctc
    22021 ctataagcgg atctataaat cacaagaata cctggcaacg ttgggcacaa ttgaataccg
    22081 atcattgttt ggcagttaca gcctgaccgt tgacgacacg gtgtttgcga tggtttctga
    22141 tggtgagttg tatcttcggg cttgtgagca aagtgcacag tactgtgtaa aacatccgcc
    22201 tgtctggctg acatataaaa agtgtggccg atccgttacc ctcaattact atcgggttga
    22261 tgaaagtcta tggcgaaatc aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc
    22321 agcgctgaaa gagaaaagca cgcgcaatac ccgggaaaga ctgaaagatt tgcccaatat
    22381 gtcttttcat ctggaagcga ttctcgggga ggtgggtaga ctggccccct gaatctccag
    22441 acaaccagta tcacttaa
//
LOCUS       DAJTRH010000061        22278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTRH010000061 DAJTRH010000000
VERSION     DAJTRH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..1025)
                     /locus_tag="OO339_002546"
     CDS             complement(<1..1025)
                     /locus_tag="OO339_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5927136.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="OO339_002547"
     CDS             complement(1127..2107)
                     /locus_tag="OO339_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5927137.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="OO339_002548"
     CDS             complement(2115..2825)
                     /locus_tag="OO339_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5927138.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="OO339_002549"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="OO339_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5927139.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="OO339_002550"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="OO339_002550"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5927140.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="OO339_002551"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="OO339_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5927141.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="OO339_002552"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="OO339_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5927142.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="OO339_002553"
     CDS             9402..9512
                     /locus_tag="OO339_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5927143.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="OO339_002554"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="OO339_002554"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5927144.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="OO339_002555"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="OO339_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5927145.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12394..13026
                     /locus_tag="OO339_002556"
     CDS             12394..13026
                     /locus_tag="OO339_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5927146.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="OO339_002557"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="OO339_002557"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5927147.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="OO339_002558"
     CDS             16558..17490
                     /locus_tag="OO339_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5927148.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="OO339_002559"
     CDS             complement(17531..18961)
                     /locus_tag="OO339_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5927149.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="OO339_002560"
     CDS             complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="OO339_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5927150.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="OO339_002561"
     CDS             complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="OO339_002561"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5927151.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20822..21091)
                     /locus_tag="OO339_002562"
     CDS             complement(20822..21091)
                     /locus_tag="OO339_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5927152.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21161..21745
                     /gene="mobA"
                     /locus_tag="OO339_002563"
     CDS             21161..21745
                     /gene="mobA"
                     /locus_tag="OO339_002563"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5927153.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21727..22254
                     /gene="mobB"
                     /locus_tag="OO339_002564"
     CDS             21727..22254
                     /gene="mobB"
                     /locus_tag="OO339_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5927154.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5515 a         5583 c         5333 g         5847 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg
    19081 tgaatattca cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc
    19141 accgtatcag catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga
    19201 ttcagctgat atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct
    19261 gcggcttttt cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg
    19321 tactcttcac ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga
    19381 atggtctggg ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc
    19441 agcgccatcg ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag
    19501 ttgacgtggt atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa
    19561 atatgcagaa cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc
    19621 acttgcggcg cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac
    19681 tgcgccgccg atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc
    19741 atgatctact ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac
    19801 ttttgcctgt tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca
    19861 cgggaaattt ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata
    19921 aaccaaacgc atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct
    19981 aaattcttca taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc
    20041 attgagcaac atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac
    20101 aaacattgga ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag
    20161 aacggtgaaa tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt
    20221 cagaaatgcc gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg
    20281 ctcaatgaga tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc
    20341 cgtctggtgc atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc
    20401 ttcgaactgg cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa
    20461 taaagtctga ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg
    20521 caacgcaaat tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa
    20581 tttgacgaca aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct
    20641 gttaagcggg gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat
    20701 ggtatccggg tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa
    20761 acattacgaa tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct
    20821 tttactcttt aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat
    20881 ctttctgcaa acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga
    20941 tcagaatgtc atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt
    21001 tctgcaaaaa ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt
    21061 caataacttc attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca
    21121 cgttagcagg gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat
    21181 aacaggcgtt gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt
    21241 gctggactta aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct
    21301 ctctcacgtc gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa
    21361 agtgattgaa gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat
    21421 gcagcaggaa gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca
    21481 tgatttagcc gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga
    21541 cggtgaacgc gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact
    21601 ggaatatctg caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca
    21661 tgcggttgat ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct
    21721 tgcccgatgg caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc
    21781 ggaaaaacta cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg
    21841 gggctgatta agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat
    21901 gagctgcgca aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg
    21961 atgacggaaa caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat
    22021 acctcaaagc tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt
    22081 gtgctgtttc gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt
    22141 attgctgtag ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat
    22201 gttgaggggc tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga
    22261 gtaggctgga tcagacgc
//
LOCUS       DAJTRH010000062        21514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTRH010000062 DAJTRH010000000
VERSION     DAJTRH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO339_002565"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO339_002565"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5927155.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO339_002566"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO339_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5927156.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO339_002567"
     CDS             complement(2649..3200)
                     /locus_tag="OO339_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5927157.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO339_002568"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO339_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5927158.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="OO339_002569"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="OO339_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO339_002570"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO339_002570"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5927159.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO339_002571"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO339_002571"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5927160.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO339_002572"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO339_002572"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5927161.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO339_002573"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO339_002573"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5927162.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO339_002574"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO339_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5927163.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="OO339_002575"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="OO339_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5927164.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="OO339_002576"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="OO339_002576"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5927165.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO339_002577"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO339_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5927166.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO339_002578"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO339_002578"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5927167.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO339_002579"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO339_002579"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5927168.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO339_002580"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO339_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5927169.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="OO339_002581"
     CDS             complement(18170..18565)
                     /locus_tag="OO339_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5927170.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO339_002582"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO339_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5927171.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="OO339_002583"
     CDS             19481..20074
                     /locus_tag="OO339_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5927172.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="OO339_002584"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="OO339_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5927173.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21514
                     /locus_tag="OO339_002585"
     CDS             20794..>21514
                     /locus_tag="OO339_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5927174.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5197 a         5324 c         5668 g         5325 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aata
//
LOCUS       DAJTRH010000063        21484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTRH010000063 DAJTRH010000000
VERSION     DAJTRH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..218
                     /locus_tag="OO339_002586"
     CDS             <1..218
                     /locus_tag="OO339_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5927175.1"
                     /translation="LLRRDRPDIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQ
                     QTPGQVENRALYPRPEGRGFTAHRIKD"
     gene            complement(257..604)
                     /gene="grxD"
                     /locus_tag="OO339_002587"
     CDS             complement(257..604)
                     /gene="grxD"
                     /locus_tag="OO339_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5927176.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            939..1766
                     /gene="mepH"
                     /locus_tag="OO339_002588"
     CDS             939..1766
                     /gene="mepH"
                     /locus_tag="OO339_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5927177.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1894..2475
                     /gene="sodB"
                     /locus_tag="OO339_002589"
     CDS             1894..2475
                     /gene="sodB"
                     /locus_tag="OO339_002589"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5927178.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2621..3790)
                     /locus_tag="OO339_002590"
     CDS             complement(2621..3790)
                     /locus_tag="OO339_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5927179.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3956..4045)
                     /gene="ynhF"
                     /locus_tag="OO339_002591"
     CDS             complement(3956..4045)
                     /gene="ynhF"
                     /locus_tag="OO339_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5927180.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            4344..5369
                     /gene="purR"
                     /locus_tag="OO339_002592"
     CDS             4344..5369
                     /gene="purR"
                     /locus_tag="OO339_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5927181.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5366..6298)
                     /locus_tag="OO339_002593"
     CDS             complement(5366..6298)
                     /locus_tag="OO339_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5927182.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            6411..7622
                     /locus_tag="OO339_002594"
     CDS             6411..7622
                     /locus_tag="OO339_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5927183.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7913..9061
                     /gene="cfa"
                     /locus_tag="OO339_002595"
     CDS             7913..9061
                     /gene="cfa"
                     /locus_tag="OO339_002595"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5927184.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9101..9742)
                     /gene="ribE"
                     /locus_tag="OO339_002596"
     CDS             complement(9101..9742)
                     /gene="ribE"
                     /locus_tag="OO339_002596"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5927185.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9957..11330
                     /gene="mdtK"
                     /locus_tag="OO339_002597"
     CDS             9957..11330
                     /gene="mdtK"
                     /locus_tag="OO339_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5927186.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11371..12627)
                     /locus_tag="OO339_002598"
     CDS             complement(11371..12627)
                     /locus_tag="OO339_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5927187.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12935..13011
                     /locus_tag="OO339_002599"
     tRNA            12935..13011
                     /locus_tag="OO339_002599"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12969..12971,aa:Val,seq:gac)
     gene            13016..13092
                     /locus_tag="OO339_002600"
     tRNA            13016..13092
                     /locus_tag="OO339_002600"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13050..13052,aa:Val,seq:gac)
     gene            13200..13505
                     /locus_tag="OO339_002601"
     CDS             13200..13505
                     /locus_tag="OO339_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5927188.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13631..15053
                     /locus_tag="OO339_002602"
                     /pseudo
     CDS             13631..15053
                     /locus_tag="OO339_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(15065..15877)
                     /locus_tag="OO339_002603"
     CDS             complement(15065..15877)
                     /locus_tag="OO339_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927189.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15881..16666)
                     /locus_tag="OO339_002604"
     CDS             complement(15881..16666)
                     /locus_tag="OO339_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5927190.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16663..17331)
                     /locus_tag="OO339_002605"
     CDS             complement(16663..17331)
                     /locus_tag="OO339_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5927191.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17395..18033)
                     /locus_tag="OO339_002606"
     CDS             complement(17395..18033)
                     /locus_tag="OO339_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5927192.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18046..20147)
                     /locus_tag="OO339_002607"
                     /pseudo
     CDS             complement(18046..20147)
                     /locus_tag="OO339_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20168..20794)
                     /locus_tag="OO339_002608"
     CDS             complement(20168..20794)
                     /locus_tag="OO339_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5927193.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(21250..21459)
                     /gene="fumD"
                     /locus_tag="OO339_002609"
     CDS             complement(21250..21459)
                     /gene="fumD"
                     /locus_tag="OO339_002609"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5927194.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5361 a         5575 c         4996 g         5552 t
ORIGIN      
        1 ggttactgcg acgagatcga ccagatattg cagtcaggta ttactacaaa ggcgttttgt
       61 ggagtcctgg ctattttgcc agtagctgcg aaggtgcgcc aatatccgtc atccgccagt
      121 acattgaaca acagcaaaca cctggtcagg tggaaaaccg cgccttatat ccccgccctg
      181 aaggacgagg ttttacggcg caccggataa aggactgacg gatgcccgtt cgcatccgtc
      241 agtattgcag gacggattat tccgcgtccg gctcttcaga cttgtattta gcggcagttt
      301 ctttgatcag ctgctgcagt tcgccacgct gatacatttc gatcacgata tcacaaccgc
      361 cgaccagctc gccgtcaacc cacagttgcg ggaaggtcgg ccagttagca tatttcggca
      421 gttccgcacg aatgtccgga ttctgcagaa tatcaacata ggcaaaacgt tcgccacatg
      481 cggcaagcgc ctggactgcc tgggcagaga aaccgcagct cggcagtttc ggtgaacctt
      541 tcatgtacag caggatcggg ttttcagcaa tctggcgttg gattttttcg atagtggtgc
      601 tcattgtctt gcttcctcaa acttctttac ggcagtaatc tgacattgta gcgggtcagt
      661 gcggcatcgg aaaataacat tttcatcacg cttttgctat tttatccctt tgctctattt
      721 gttgcatttc aaatattcgt ttcttctctg atgcgcggca aactggcgta tttataacca
      781 tttttagctt tcactgctgc atttttttgc gctcgccaac gaaacgtatt ttttaacaat
      841 aaaagctatt aactttctct tcttctatgc attagaatca tcaagttttg taaatcagac
      901 gcaggcatga tagacctgcc ttaacagagg gacgctcagt ggcgcggata aaccgtattt
      961 cgatcacgct ctgtgcttta ctttttacca ccctgccttt aacgcctatg gcccatgctt
     1021 caaagcaagc cagggagagt tctgctacca ctcatatcac caaaaaagca gataaaaaga
     1081 aaagcacggc aaccaccaaa aagacccaga aagccagcca gaaaacagcg aaaaaagccg
     1141 ccagtaaaag tatgaccaaa agcaaaaccg cttcttctgt taaaaaatct tccattaccg
     1201 cttctaaaaa cgccaaaact cgcagcaaac acgccgtcaa taaaacggcc tcagccagct
     1261 ttaccgaaaa gtgtaccaaa cgtaagggct ataaatcgca ttgtgtgaaa gtcaaaaatg
     1321 ccgcgtcagg aacccttgcc gacgcgcaca aagcgaaggt gcaaaaagcc acgaaagtgg
     1381 caatgaataa actgatgcag caaattggta agccatatcg ttggggcggc agctcaccgc
     1441 gtaccggttt tgattgcagc ggcctggttt attacgctta taaagatttg gtgaaaattc
     1501 gtattccgcg tacggcgaat gaaatgtatc acctgcgtga tgcaggacct atcgaacgta
     1561 gtgagctgaa aaacggcgac ctggtctttt tccgtactca gggacgcggt acagccgatc
     1621 atgtcggcgt gtatgtcggc aacggcaaat ttattcagtc accgcgcaca ggtcaggaaa
     1681 ttcaaatcac ttctctcagt gaagactact ggcagcgcca ctatgttggc gctcgtcggg
     1741 taatgacccc aaaaacactt cgctaaaact ttaccctgtt gttacggcaa cagggtaagt
     1801 tcatcttttg tctcaccttt taatttgcta ccctatccat acgcacaata aggctattgt
     1861 acgtatgcaa attaataata aaggagagta gcaatgtcat tcgaattacc tgcactacca
     1921 tatgctaaag atgctctggc accgcacatt tctgcggaaa ctatcgagta tcactacggc
     1981 aagcatcatc agacttatgt cactaacctg aacaacctga ttaaaggtac cgcgtttgaa
     2041 ggtaaatcac tggaagagat tattcgcagc tctgaaggtg gcgtattcaa caacgcagct
     2101 caggtctgga accatacttt ctactggaac tgcctggcac cgaacgccgg tggcgaaccg
     2161 actggaaaag tcgctgaagc tatcgccgca tcttttggca gctttgccga tttcaaagcg
     2221 cagtttactg atgcagcgat caaaaacttt ggttctggct ggacctggct ggtgaaaaac
     2281 agcgatggca aactggctat cgtttcaacc tctaacgcgg gtactccgct gaccaccgat
     2341 gcgactccgc tgctgaccgt tgatgtctgg gaacacgctt attacatcga ctatcgcaat
     2401 gcacgtcctg gctatctgga gcacttctgg gcgctggtga actgggaatt cgtagcgaaa
     2461 aatctcgctg cataataact gatggcaaat gcagcattgc ctgatgcgct tcgcttacca
     2521 tgccaacgcg gatgaggcgt taacagcgca ttcggcaaga taaatcgcac gttgtcagca
     2581 actgtaacgc agaaggttat ccttctgcgt ttttgtttaa ttagctgtta gcaacgcaaa
     2641 ctgtttcagg ttgttttctg gctgacataa acaccagcaa taatgccagt cccgcgacaa
     2701 tcgcccccgt caccggcaca aagctgtatc ccagcccagc ggaaattacc gcaccaccag
     2761 cagctgctcc cagcgcattt ccaagattaa aggcaccaat attgactgat gaagacagac
     2821 ctggcgcttc actggcgaca tgcatcacgc gcatctgtaa cggaggtaca accgcaaagg
     2881 ttgctgcgcc ccacaccacc atgctaatag ctgcgccgaa ctcattgcgg gccaggaacg
     2941 ggattgccag cataatcacc atcagcagca acaaaaagcc tttcaacgtg ccgttaactg
     3001 aacgatctgc cagtttgccg ccgagatagt taccgataga gaatccgaca ccaatcagca
     3061 ccagcattgc cgtgacgaac accggtgttg cgtgggtaat actttgcagt accggagaga
     3121 tataggtgta gagggtaaac attgcaccag ctcccagtac cgtcgtcagc aatgcagaca
     3181 gcacctgcgg acgcattaat accgccagct cttttttcac ttcaggtcgt gcccctgtac
     3241 cacctttagg taatgagaag aacagactta ccattgaaat cactcccagc cccgccgttg
     3301 ccagaaatga catccgccag ccgatggttt cacccaacca ggtcgccgcc ggcacgccac
     3361 cgatatttgc cagggttaac cccataaaca tagtggcaac tgcgctggcc tgtttatgtt
     3421 ttggcaccac gcttgcggcc acgactgaac ccaaaccaaa aaatgctccg tgattcaggc
     3481 tggtcaaaat acgtgaaagc atcagggtca tataatccgg cgcgatggcg gaaagaacgt
     3541 tgcctagcgt gaaaattgcc atcaggaaaa tcaacgcact gcggcgggca cgatgagaaa
     3601 gtagaagcgt catcagcggc gcgcccacca ttacgccaac tgcataggca ctgattaaca
     3661 ttccggcagc gggaatcgag acatccacac cgcgcgcaat gacgggcaac aagcccattg
     3721 gcgagaactc cgttgtcccg ataccaaacg cgccaatcgc cagcgccagc aacggatagt
     3781 taattttcat gccttatctc cacctcttcg cgtcattacg cgatattcat taaagtggcg
     3841 aaagcatgac agcaatcaca aaaaaatgaa aataacaaaa agagaaaaca cttttgccat
     3901 tttgctaaca aacaggaagg agatgcgagg gagaacgcgc tccctcgaga ggaaatcagt
     3961 gcagcgcggc agtcaaaccc acggctacga tcaaaccgag gacgataacc gttgttacca
     4021 gtgaaaattt aaggtcggtg ctcatcaagt tttctccttt tttattacca cacaaaaagt
     4081 gatattacgc atttttacac actgtgatga aaaaatctcc cgtcatttat aatgataagt
     4141 gtttttacca cttccccttt tcgtcaagat cggccaaaat tccacgctta cactatttgc
     4201 gtactggcca ttgacccctt cctgacgctc cgtgtcgttt ttccggcgta ccgcaacact
     4261 tttgttgtgc gtaaggtgtg taaaggcaaa cgtttacctt gcgattttgc aggagctgaa
     4321 gttagggtct ggagtgaaat ggaatggcaa caataaaaga tgtagcgaaa cgagcaaacg
     4381 tttccactac aactgtgtca cacgtgatca acaaaacacg tttcgtcgct gaagaaacgc
     4441 gcaacgccgt gtgggcagcg attaaagaat tacactactc ccctagcgcg gtggcgcgta
     4501 gcctgaaggt taaccacacc aagtctatcg gtttgctggc gaccagcagc gaagcggcct
     4561 attttgccga gatcattgaa gcagttgaaa aaaattgctt ccagaaaggt tacaccctga
     4621 ttctgggtaa tgcgtggaac aatcttgaga aacagcgggc ttatctgtcg atgatggcgc
     4681 aaaaacgcgt cgatggtctg ctggtgatgt gttctgagta cccagagccg ttgctggcga
     4741 tgctggaaga gtatcgccat atcccaatgg tggtgatgga ctggggtgaa gcaaaagctg
     4801 acttcaccga tgcggtcatt gataacgcgt tcgaaggcgg ctacatggcc gggcgttatc
     4861 tgattgaacg cggtcaccgc gaaatcggcg tcatccccgg cccgctggaa cgtaacaccg
     4921 gcgcaggccg ccttgccggt tttatgaagg cgatggaaga agcgatgatc aaggtgccgg
     4981 aaagctggat tgtgcagggt gactttgaac ctgaatccgg ttatcgcgcc atgcagcaaa
     5041 tcctgtcgca gccgcatcgc cctactgccg tcttctgtgg tggcgatatc atggcaatgg
     5101 gcgcactttg tgctgctgat gaaatgggcc tgcgcgtccc gcaggatgtt tcgctgatcg
     5161 gttatgataa cgtgcgcaac gcgcgctatt ttacgccggc gctgaccacg atccatcagc
     5221 caaaagattc gctgggtgaa acagcgttca acatgctgtt ggatcgtatc gtcaacaaac
     5281 gtgaagaacc gcagtccatt gaagtgcatc cgcgtttgat tgaacgccgc tccgtggctg
     5341 acggcccgtt ccgcgactat cgtcgttaat cacccgttgc gggagtctct tccggctccc
     5401 gcagccactc cttattcagc gtctcactat cgctgagata ctcaagcagc caggttaacg
     5461 caggcgacat atcattttgc tgccatgtca gacaacatgc cgaatccggg aaggggtttt
     5521 ccagttcaag tgctacccac ttcccctcat taagccacgg tttggcgaaa tgtgttggca
     5581 ccatccctat gcataatcct gccgagatac aggttgccga tgattcccag tcaggtacga
     5641 caagtctttt ttggttatcc agcaaccagg taatacgttt aggtagcgtt cgcgaggtgt
     5701 cttcgcgcac caacgacggc cagttgcgca acgtatcatc gctgaacggg ccatccatca
     5761 acgccagcgg gtggtgactg gcaacaacgc aactccagct tagcatcccc atatcccgga
     5821 aggcataacg accgcctacc ggaatcgcgc gtgttgcgcc aattgccagt tccacgcgcc
     5881 cgtcggaaag cgcatcccag acaccgttga acacttcctg aaagacaaga agttcgacat
     5941 catcaaaatg gcgataaaaa tcaacgatca tctgccgtgt acgttctggc ctgacaatat
     6001 tatccactgc gatagctaac tgaccgcgcc agccgttcgc tatctgctga cattgctggc
     6061 gggtgatctg catttttttg acaacagagc gcccttcttt gagaaaccac gctccagcag
     6121 cggtcagctc cacatcacgg tgccgtcgtt caaagagcgg taccgccagc cactcttcca
     6181 gctgacgcac ggtatagctg accgcagaag gaacacgatg cagctcctgt gccgcagcgc
     6241 taaaactacc attacgcgct accgcatcaa caacttcgag tgaatattct gaccacatag
     6301 tctgcctgca aaatttttga aaccagtcat caaatattac cgtttcacaa cactaatttc
     6361 actccctaca ctttgcggcg gtgtttaatt gagagattta gagaatatac atgcaacctg
     6421 ggaaaagatt tttagtctgg ctggcgggtt tgagcgtact cggttttctg gcaaccgata
     6481 tgtatctgcc tgctttcgcc gccatacagg ccgacctgca aacgcctgcg tctgctgtca
     6541 gtgccagcct tagtctgttc cttgccggtt ttgccgcagc ccagcttctg tgggggccac
     6601 tctccgaccg ttatggtcgt aaaccggtgt tattaatcgg cctgacaatt tttgcgttag
     6661 gtagtctggg gatgctgtgg gtagaaaacg ccgccacgct gctggtattg cgttttgtac
     6721 aggctgtggg ggtctgcgcc gcggcggtta tctggcaagc attagtgaca gattattatc
     6781 cttcacagaa agtcaaccgt atttttgcga ccatcatgcc gctggtgggt ctgtctccgg
     6841 cactggctcc cctgttagga agctggctgc tggtccattt ttcctggcag gcgattttcg
     6901 ccaccctgtt tgccattacc gtggtgctga ttctgcctat tttctggctc aaacccacga
     6961 cgaaggcccg taacaatagt caggatggtc tgacctttac cgacctgcta cgttctaaaa
     7021 cctatcgcgg caacgtgctg atatacgcag cctgttcagc cagttttttt gcatggctga
     7081 ccggttcacc gttcatcctt agtgaaatgg gctacagccc ggcagttatt ggtttaagtt
     7141 atgtcccgca aactatcgcg tttctgattg gtggttatgg ctgtcgcgcc gcactgcaga
     7201 aatggcaagg caagcagtta ttaccgtggt tgctggtgct gtttgctgtc agcgtcattg
     7261 cgacctgggc tgcgggcttc attagccatg tgtcgctggt cgaaatcctg atcccattct
     7321 gtgtgatggc gattgccaat ggcgcgatct accctattgt tgtcgcccag gcgctgcgtc
     7381 ccttcccaca tgcaaccggt cgcgccgcag cgttgcagaa cactctgcaa ctgggtctgt
     7441 gcttcctcgc aagtctggta gtttcctggc ttattagtat cagcacgcca ttgctcacca
     7501 ccaccagcgt gatgttatca acagtagtgc tggtcgcgct gggttacatg atgcaacgtt
     7561 gtgaagaagt tgactgcccg aatcatggca atgccgaagt cgctcatagc gaatcacact
     7621 gatctatatc gatatactta tacttaggct gctaacaaaa ttttgttgta tgattgaaat
     7681 tagcggccta tactaatttc gagttgttaa agctacgata aatattatgt ttttacgggg
     7741 acaggatcgt tcccgactca ctatggatgg tcatttcggc aagggttcct cctttccctc
     7801 tgttctacgt cggattatag actcgcggtt ttttctgcga gatttctcac aaagcccaaa
     7861 aagcgtctac gctgttttaa ggttctgatc accgaccagt gatggagaaa ctatgagttc
     7921 atcgtgtata gaagaagtca gtgtaccgga tgacaactgg taccgtatcg ccaacgaatt
     7981 acttagccgt gccggtatag ccattaacgg ttctgccccg gcggatattc gtgtgaaaaa
     8041 ccccgatttt tttaaacgcg ttctgcagga aggctctttg gggttaggcg aaagttatat
     8101 ggatggctgg tgggaatgtg accgactgga tatgtttttt agcaaagtct tacgcgcagg
     8161 tctcgagaac caactccccc atcatttcaa agacacgctg cgtattgccg gcgctcgtct
     8221 cttcaatctg cagagtaaaa aacgtgcctg gatagtcggc aaggagcatt acgatttggg
     8281 taatgacttg ttcagccgca tgcttgatcc cttcatgcaa tattcctgcg cttactggaa
     8341 agatgccgat aatctggaat ctgcccagca ggcgaagctc aaaatgattt gtgaaaaatt
     8401 gcagttaaaa ccagggatgc gcgtactgga tattggctgc ggctggggcg gactagcgca
     8461 ctacatggca tctaactatg acgtaagcgt ggtcggcgtc accatttctg ccgaacagca
     8521 aaaaatggct caggaacgct gtaaaggcct ggatgtcacc attttgctgc aagattatcg
     8581 tgacctgaac gaccagtttg atcgtattgt ttctgtgggg atgttcgagc acgtcggacc
     8641 gaaaaattac gatacctatt ttgcggtggt ggatcgtaat ttgaaaccgg aaggtatatt
     8701 cctgctccat actatcggtt cgaaaaaaac cgatctgaat gttgatccct ggattaataa
     8761 atatattttt ccgaacggtt gcctgccctc tgtacgccag attgctcaat ccagcgaacc
     8821 ccactttgtg atggaagact ggcataactt cggtgctgat tacgatacta cgttgatggc
     8881 gtggtatgaa cgattcctcg ccgcatggcc agaaattgcg gataactaca gtgaacgctt
     8941 taaacggatg tttacctatt atctgaatgc ctgcgcaggt gctttccgcg cccgtgatat
     9001 tcagctctgg caggtggtgt tctcacgcgg tgttgaaaac ggccttcgag tcgctcgcta
     9061 aaggctattc tatcgccccc tcttcggggg cgagttcaga tcaggcttct gtacctggtt
     9121 gattcatggc attttctcgt gccgccagca cacgttctac cgtatctacc actgcctgag
     9181 tttgtggatc gatttcaatg ttgacgcgtg cgccaagttt tttcttccca agagtcgtgc
     9241 gttccagtgt ttccggaatt aaatggacgc aaaaacgcgt tggcgtgact tcgccgacgg
     9301 tcaggctaat accgtcgatg ccaataaatc ctttgtacag gatatatttc atcaactgac
     9361 tatcctggac tttaaaccag atctggcgat tattttccga ggttaatatt ttcgccactt
     9421 cagcggtggt cataatatga cctgacatta agtgtccgcc aatttcatca ctgaatttcg
     9481 ccgcacgctc aacgtttacc caatccccca cttttaaatc gccaagattg gtaatgcgta
     9541 acgtttcttt catcaggtca aaactgacat ggttgccgtt aatttccgtc acggtcaggc
     9601 agcaaccgtt atgcgccacg gaagcaccgg tttccaggcc gtccagcatg tggtcgggta
     9661 actccaccac atgcgtacga aaatttggtt tctcgtcaat cgacaccagt tttgcggtgc
     9721 cctgtacaat ccccgtaaac atacttacaa ctcctgaaat cagttaagac attctgttca
     9781 gcacaatagc aggtggaaaa cgcccttacc agtgaagggg taagaatggc tattttttca
     9841 ctggagaatt aataaatcct cgctacaata gactgaattt cccctgcttc ttctttttgc
     9901 tgcccattca ggcggctttt tagtctctca tataactaca aataaaaggt gttcacgtgc
     9961 agaagtatat cagtgaagcg cgtctgttat tagcattagc aatcccggtg attctcgcgc
    10021 aaatcgccca aactgcgatg ggttttgtcg ataccgtgat ggcgggcggc tatagtgcca
    10081 ccgacatggc ggcggtcgct atcggtactt ctatctggct tccggcgatc ctctttggtc
    10141 acggactgct gctggcatta acgccggtta tcgcgcaatt aaatggttcc ggtcgacgtg
    10201 agcgcattgc gcatcaggtg cgacaaggtt tctggctggc aggttttgtt tccgttctca
    10261 ttatgctggt gctgtggaat gcaggttaca ttatccgcta catggaaaac atcgatccgg
    10321 ctctggcgga caaagccgtg ggttatctgc gtgcgttgtt gtggggcgcg ccgggatatc
    10381 tgttcttcca ggttgcccgt aaccaatgtg aaggtctggc aaaaaccaag ccgggtatgg
    10441 taatgggctt tatcggcctg ctggtgaaca tcccggtgaa ctatatcttt atttatggtc
    10501 atttcggtat gcctgagctc ggtggcgttg gttgtggcgt ggctactgcg gcggtgtatt
    10561 gggtcatgtt ccttgccatg gtttcttaca ttaaacgcgc ccgctccatg cgcgatattc
    10621 gtaacgaaaa aggcaccgct aagcccgagc ctgcggttat gaaacgactg attcaactcg
    10681 gtttgccgat tgcgctggca ctgttctttg aagtgacact gtttgccgtc gtggctctgt
    10741 tagtgtctcc gctcggtatt gttgatgtcg caggacacca gattgccctg aactttagtt
    10801 cactgatgtt tgtgcttcca atgtcgctgg cggcagcggt aactatccgc gtaggttatc
    10861 gtctgggtca gggctcaacg ctggatgcgc aaaccgctgc gcggaccggg cttatggtgg
    10921 gtgtctgtat ggcaaccctg acggccattt tcacggtttc actgcgggag caaatcgccc
    10981 tgttgtacaa cgacaatccc gaggttgtaa cgctggctgc gcatttgatg ttgctggcgg
    11041 cggtatatca gatttctgac tcaatccagg tgattggcag tgggattttg cgtggttata
    11101 aagatacgcg ttccattttc tatatcacct ttacagctta ctgggtgctg ggcttgccaa
    11161 gcggctatat tctggcactg accgatctgg tcgttgaacc tatggggcca gcaggcttct
    11221 ggataggctt tattatcggc ctgacatcgg cagccattat gatgatgctg cgtatgcggt
    11281 tcctgcaacg tatgccgtca gccatcattc tgcaacgagc gtcccgctaa taaagacaag
    11341 gcgcaacctt cacgggttgc gcctgtgttt ttacgcaggc tggagcgttg cgccaatccc
    11401 gtcttcgtct ggctgtaatt tcagagcgtt acacagagtt tgccgactgg catctgttat
    11461 ctcaacgcgt atatccccgt taacttcccc tttcacattg atgaagtcag gtgaaatttc
    11521 tttcgcactg gtttgcaggc aaatcgctcc ctctccgcgc aggcgtttca ctttcagttg
    11581 cacatgcgtt gccgcaccag tgatcagcca cagttttccg ccattcagat tgatgttacc
    11641 aacaatgaaa gtgacgctgt caccttttac cgtactgccg tcgagaatca acacaccgcc
    11701 ctgctgtaca gtaatcgttg attgttgcag ctctgcgcca taaccaataa tcattgaagc
    11761 gccgtccagt aatgcaacac tcccggtcag ttcaaccggt gcgcgatctt cctctacgac
    11821 aaaattttcg tccgcgctgg tgggtgacaa caggatcact gttccacctt gtattaccgc
    11881 attttccagc gtaccggcat gcacttcagc ccgccccccc acattcaccg acaggttttg
    11941 cgtcttaccg gaactgtaga gctgaaggcc atccgtcccc agacgataaa tggatccatt
    12001 ctcaacaata gtgtcagagt cttcaccacc gagattattc atggtgccac cagcaagcaa
    12061 cgtatcactg gctttcccgg ccagcataaa aacgccgccc tgattaatct cacaattgct
    12121 ggcttctcca cttggataca ccaacaattc accaccttca tctaccacga ccgctgaggt
    12181 tgtcccatta accaacaggc cgccctcttg atcgagaatg attttttccg cgcgatgtcc
    12241 ttccagtaca cgcaggttac cgccattttc cagcaacaaa ccgcaggcat aaccctgatc
    12301 aacgctgaat tcaccttcgg gatggcggcc attaaccgta gcgagcgtag agagactaat
    12361 tgcgcctccc tcggactgaa caatatctgt ggcaatggca ccgtcgctga ccgatagcgt
    12421 gccattgtta ccgtactgtg tgcatttggc gatcccaccg gttgccactt tcaaccagcc
    12481 agcccccgtg accacggtat tttcggctct tccgccagct tcaacaatca actcgccttc
    12541 agtaagttgc gtcgcgccaa ggacctcgcc ggtcttaaca atttgcacat tcccgtcgct
    12601 catcaagttt ttcgcatcag atcccatgat ttattccttt gctgcatctg tgtgccttta
    12661 ttgctaccta agtgtaaagg ctacggagga tttatccacg acagatttga gatggtggca
    12721 aacaactctg tttaaactct gatacacgaa ttattgggtt gtatcagatg taaatgcgat
    12781 cctgaataaa aatcgccctt gcaaatcaac aaaatatagc caatttgctt aaaagctctg
    12841 caaacggtcg aaatagcgca gaaaattacg ttttgcctct tgccaccttt ccactctgcc
    12901 gctaatattc gtccccgttg tcacctacaa cgttgcgttc atagctcagt tggttagagc
    12961 accaccttga catggtgggg gtcgttggtt cgagtccaat tgaacgcacc atcctgcgtc
    13021 cgtagctcag ttggttagag caccaccttg acatggtggg ggtcggtggt tcgagtccac
    13081 tcggacgcac cagattttct taatctggtc ttttcctttt tccctctgtt tcttctctgt
    13141 atccaatacg ttaaaagatt tacactgtct tcgtatgcgt tatcagaagg agaatcgcta
    13201 tggcaacttt gttacaactt cattttgctt ttaatggccc atttggcgac gcaatggctg
    13261 agcagcttaa accacttgct gagtcgatta atcaggaacc tggttttctg tggaaggtat
    13321 ggacagaaag tgaaaagaac cacgaagccg gtgggatcta ccttttcact gatgaaaaaa
    13381 gcgcccttgc ctatctggaa aaacatactg cccgactgaa aaatctcggc gttgaggaag
    13441 ttgtcgccaa agttttcgat gtcaatgagc cacttagtca aatcaatcag gcaaaactcg
    13501 cctgacagaa tttaatcaag ggcggttagc gcccttttca tccctgtctg aaatttctca
    13561 aattctaaaa atctcaacca aacttatctg ataacactaa attcgaaaga atgcgtacag
    13621 gtaagtaaca atgaaaaaaa ttgctattgt gggtgccggg cctacgggga tctacacctt
    13681 attctcgctt ctacagcaac aaactccact ttctatttct atcttcgagc aggctgacga
    13741 ggccggtgtc gggatgccat acagtgatga ggaaaactca aaaatgatgc tggcaaatat
    13801 tgccagtatt gaaataccgc cgattaattg tacgtatctc gaatggctac aaaagcaaga
    13861 agccagccat ctccagcgtt atggcgttaa aaaagaaacc ttgcacgatc gtcagttttt
    13921 accgcgaatt ctgctgggcg aatatttccg cgatcaattt ttacgattag tagaccaggc
    13981 acgacagcaa aaatttgcag tggctgttta tgaatcatgc caggttaccg atctgcaaat
    14041 tacaaatgct ggcgtcatgc tcgctacaaa tcaggattta cccagcgaga cgtttgattt
    14101 agcggtgatt gccacgggtc acgtctggcc tgatgaagaa gaagcaacca gaacgtattt
    14161 tcccagcccg tggtcaggcc tgatggaagc aaaggtcgat gcgtgtaacg tgggtattat
    14221 gggaacatcc ttgagcggac tggatgcggc aatggcagtg gctattcagc atggttcgtt
    14281 cattgaagat gataaacaac acgtcgtttt tcaccgcgat aacgcaagtg aaaagctaaa
    14341 tattacgttg atgtcgcgca cgggtatttt acccgaagcc gatttctatt gccctattcc
    14401 ctacgagccc ttacacatcg tcaccgatca ggcattaaat gctgagattc aaaaaggtga
    14461 agagggcctt ttggatcggg tatttagatt gatagtagag gaaatcaagt ttgctgatcc
    14521 agactggagc caacgcatag ccttagagag cctgaatgtc gattcctttg ctcaagcctg
    14581 gtttgccgag cgcaaacaac gcgacccatt tgactgggca gaaaaaaatc tccaggaagt
    14641 cgaacgcaat aaacgagaaa aacatactgt tccctggcgt tatgtcattc tgcgcctgca
    14701 tgaagccgta caggaaattg ttcctcatct gaatgaacac gaccataaac ggttcagtaa
    14761 aggccttgcc agggttttca tcgataatta tgcggcaatc ccttcagagt ctattcgtcg
    14821 actactggcc ttacgtgaag cggggatctg aaatccctga tgttggcgaa gattatacgt
    14881 tacagcaacc cgaagatatt cgtggacgcg tagcgttcgg cgcgttgccc tggttgatgc
    14941 acgaccagcc tttcgttcag ggacttacgg catgtgcaga aattggtgag gcgatggctc
    15001 gggcggtcgt aaagcctgca tcccgtgctc gtcggcgtct ttcgtttgat taaaggtaaa
    15061 gctatcagca ctcaacaaac tgatgctgtt cattaagacg atagcggact ccggttcgaa
    15121 tattgttttc accttcaatg gcgacggcga agcgaactct ttcgccgtca tgatatgcca
    15181 gtgcggcgct tccccctagc ccgagaataa ttgagtcgac cacgcccgtg ctcactaccg
    15241 ttgaattttc accgctggca atgatgtgta cattgtcgcc gctgttggca attctggcgt
    15301 ttgcgccaaa gctggcgatt tgcaccaggt caccattact ggcgacatga caccgttcac
    15361 ccaatgtaca aacgcgtact cgcattccgg tattcgcaat ccgactacta tctccagcgc
    15421 tgcttatccg cgaagaatta ccggttacag caacccgtgc gcgttcaccg gagctgccaa
    15481 tgtgactgtt aaagccgaca cttccaatcc gcacgctata acccatactg gcaatttgcg
    15541 cggcataccc agcggttgcg attcgggcgt tatcctcaca acagccgatg cgagcatgat
    15601 cgtcgctgcg ggcaaactga ctgcaaaatg gtatctctcc ggggtctgtg gccagttttt
    15661 gcatagagga aacttcctga tgcgcgaagt tttcatccgc cagccatttt ttccacgcat
    15721 actcaaccag actctccgcc cagtccgtat atccttcctc aatcagtgca tgacgaatat
    15781 cggcataact accgccgcgt gggaaatggc gcaggaacca gcgatacatc accgctccga
    15841 tacgccattc tcgcaggtct gctcgtgtaa tgatcattcg ttagtgccgg tgatagccat
    15901 cgaccatgct tttaaaggtt tcgtgtggcg tacgccccgt ggtgcaaaga taaagatgac
    15961 cgaagataaa aaagaggctt ataaatgcca gagcaaaatg cgcctgcaat aaccagtatc
    16021 ttacgccagg aaacacatct cccacggctt gcggatagag acacaacagc cccgtcaata
    16081 gtaacaacgg cagcaatcca tacatgacac caacataggc gacctgctgt aagggattaa
    16141 atttagactg tgttgttgcc gggaaaggat gttcttcccc ctgcataatg ccaaacaaat
    16201 aaaatcgcgt ttgttttgcc gctcgttcca gccacccctg acgacgaatg cgatagtggt
    16261 gaccattatc cccaacggca ttgatcagca caaagccgag ccagcatgcc agtaacaaaa
    16321 atccgcaaac ttcatgcacc gcaaccagac ttttaaccgc agttgcgccc accatcgcaa
    16381 agtgatttat cagcccactt gccagcaaca atacaaagag taacgcattc gaccagtgcc
    16441 ataatctgac cgctttgctg taaagataaa ccttctcacc atgatcggtg gctgatttct
    16501 tcacgccacg agcacgaagc aaggcgtgca gccccagcac caaccacatc ccaaccagca
    16561 atactccggc aatgatcaac cacaccggcc aaaattcggg agtgaactgt ggcacatagt
    16621 tcgctaactg gctctgaaac tgttcagcat gttgcgacgg gttcatacat tttccttttt
    16681 aatcaaatgt tgaccgaacc ggcgatacag atgcggtttt ccggcaccag gtagctgata
    16741 ttgatagtat ttattatcct gcaaccacgc ctgaatttct gggctatctt cgcgaccaaa
    16801 gatcaatgcg tgttccggac aggcgctgac gcaaatgggc ggaaagcctt ttgccagtcg
    16861 ggactcagcg cagaaatcac atttatctgc cactttagtg acagggttaa ggtagcgaac
    16921 caggtacgga caagcgccaa tgcaataact gcagccgatg cactgcgatt tttctacccg
    16981 cacgatcccc tgctcatcac gccacgatgc ccccgtcggg catacgtcga tgcacggagc
    17041 atcttcgcag tgctgacatg actggcgaaa aaagtgatac tgcgtttcgt tgtcgttatc
    17101 ggtgactgga atgtgtgcta tcgataaacg gcttccctga gcggggacgt ggttagtttt
    17161 acggcatgcg cgggcgcaaa tattacagcc attgcaccgt gactcgtcat gaatcatggc
    17221 ataccgaact tccttttctt gcctcgtgtt cgctaacaga gaagacgcag aaccagtaaa
    17281 aaagattact gttcccatcc ccagaacaaa tttgcgtcga gtgaaggaca tctgattatc
    17341 ccctaatgca gtggctgcaa ctgtccaccc tatccatgac atctccctga aatttcaggg
    17401 gttctgaaac tgctctacct cgaaccattc gcataagcca taagccttgc cataggtcat
    17461 aaatcgctcg ctaaacaagt acaacaccgc cgtcgatgac tccacctgcc gtatatcggc
    17521 atattcaggt gcaacatcca tcgcggcgat cgcggcttca atctgcgctt cctggaagta
    17581 atatggcgct tgcatcagca tggcgacttt gtaaggacga ggatacgtct ggcactcaaa
    17641 acgaacggcg tgtgcaatgg cgcgacaaat gtcctgctcc acaacttgca aagacatcgc
    17701 cgcgtagttt tcactcatgg cctgcgttga gtaatagtaa tcatcctgac ttcccgtcat
    17761 acaagcgata tccgcgtagt ctccgttctg cttaatgctt tccagcaaaa ctgacagttc
    17821 ctcttctgca accgaatagg gtggcgagag aaacacggtg cgcgccacca gctgcccagc
    17881 cgcagaatgc tggcgaatga actcagccag gatttcagca ggttctggtt cggttaccgt
    17941 atcaacgggg tgacgcatcc cctgcaacca ttgccgcttc gcctcgtcaa cttcgctgac
    18001 tttcgctaac ggcaattcat cctgatggtt catcttaccc attccttacg caggcagtag
    18061 attgtgtgct gccagatcgg cggcaatatc ttccagcccc agacgctgca atgtttcgcg
    18121 ggttggacaa ccaagctgtg ggtcccagcc catttctttg tagaacatcg tgagcgaagc
    18181 atgcatatcg tcacgatcca ttttgtcagt accttcagta aagaccggga tctgcggatc
    18241 cttgtcgaaa acccaggaac agataagatc gtgttcatta cgcatatctt tggtttgcat
    18301 cggttttacc gtgtaggcac gatgcaacgt aaagatacgc tctgcagcta aatctaattt
    18361 ttcctgagtc atctcttcgc cggtgatcgc tttgaagaat ttggcttcaa gcgccagatc
    18421 gccccggtaa ttacggcttt tcagcggcga aacggtcatt ggccagaccc agttgcacag
    18481 agtgacggcg ttatgcaaac agacccgcaa cagcgaccac tttgcatatt taatttttgc
    18541 gtcattgatt ggcgtgtagt ttttggtttc atcgtaagca tcttcagaac caaaaagttc
    18601 tttcgccact tcacgttgca gtttcaatgg caagccggaa ccaataaagt tgatatgggt
    18661 atgcgtcatg cagtcacggt tgaacataca cttaacaatg gaaccgacct gcgctgacgc
    18721 ttcattggca tggtgaaccg gatagccaaa cggcgaccag agtttatttt tcgcgtagcc
    18781 ccagtactct ttacccaaat tccagcgttc tgcgatggca tatgaaccat cagccaggtg
    18841 actcagctca cccacacgat gcgccagacg gtagtaaaaa tctttaatga agttaacgtc
    18901 acccgcttcc agttgatccc agcgaatttc tgcatactct tcagctggca gaacacgctt
    18961 gaacacacct ttgctgtaac aataagtaaa gtcgcgatgc aactgcccgt agttacacca
    19021 taggccgtag tcatcgaaca aattcagacc aaccaggtta ccaatcacac gaccatcgtc
    19081 tttatcttca aaatctttcg gcccgttcgg gaagatggtg gtatggacaa agtttgctac
    19141 acaagtgtta ccacctgtgc tgggcacgcc aaactctttc acccgaggaa tattcatttg
    19201 ggtcatacaa cggatcgggc aagagtggca gccgctcatt tttactgtgt atttctctgc
    19261 cgccggtcct aagtcaaaaa cggatttata ggtacgaaag ccgaccgtat tctgattgcc
    19321 tggcggaatt tcacccgttt caatcggacc accttcagcc gcgccccaaa acagcccttt
    19381 acgtgctgtc cagcgtgact tgggatctga atactctgcc cacgattgtg gcgtacttgg
    19441 cacgacatgg ttgttattcg caccaataag ttcagtcatc atgtaatcat tgagacgctt
    19501 catctcctga cgatcggcaa tgttgacccc tttcgtccct tcaaccgcaa tcgctttcag
    19561 gtttttcgaa cccattattg cgccagttcc cgcaccgccg ctgtggttac ggctattcaa
    19621 catgccagag agaggaataa ggttttcccc agcctgacca atagccgcca cacaggtttc
    19681 cgggctggtc aatcgacaaa tttcttccgt cgttgcgcgc gtcccttttc cccataagaa
    19741 atcggctttt tccaggctaa ctttgtcatc tttaatcttc agccataccg gtgatttcgc
    19801 cttcccttcg ataataatga cgtcgtatcc agcgaatttc atttgcgctg caaaaaagcc
    19861 acccatatgg gcatcgcgac taaatttcct ttggtaaaag tagaaagtga ggtgatattt
    19921 acgcgagaac tacagggggc accagatcca ggtaatgggc cggtagcaaa gactaattta
    19981 ttcgcttcat cgaaaggttt cgtgcctagc ggtacttcgt catacataat tttgtagccg
    20041 aagcccatgc caccgacaaa acttttaaac ttactggaat cttcgagggt aatatttcct
    20101 gtcgtgagat tgactcttaa tatattacct gtccaaccgt tagccatgat tttttccttt
    20161 gcaagattca cacagtaata tctttccact cgataatttt taacgcccct gttgggcagg
    20221 cgtttgcgca ttcaccgcat aacacacatt tcgaggattt tttactttcg gtatttacgg
    20281 tggccatcat ccacgggcat gccgtagtac aggcgctaca gccaatacaa cgtttatgat
    20341 cgacggtaat acagccttct ttctgctgcc aggtaatcgc accaatcggg cagacgttca
    20401 tgcattgcgg ctctttgcat tgacggcagg tgtccgcggt atagttgaga tcgccataca
    20461 ggccgccgcc agagccaacc ccgttgtcgc caaagaaata attgcgatgg attttgatac
    20521 gggagaagaa tgtccctact gagccatcgt tgaagttggt acaagagatt tcacagcggt
    20581 ggcaacccgt gcatcgcgct cgctgggtca ccagcacccc tttgggtgta tttatcaacc
    20641 ctactgtgcc gctatcaata tcttcctgtt tgcaacccaa aagtgacagt aacgcagggg
    20701 caattgttag accagcaagg ccttttcctg atatacgcaa gaattctaat cgcgttaagc
    20761 caatatctaa tagtggacga tcaaccgggt tcatttattt cgcatcctct tcgcaaacac
    20821 agtgctgaca attcagcagc ctgatattaa gcattcaact aatacgctgc tgactctgtc
    20881 cccaacacca gatattttcc ctaacccgat agtgtatgag aaaagattta acgccgataa
    20941 actaatcctt tatggttagg agaaaaaata atgtgattat tctgagccct taacattgat
    21001 cgttatcaat taaaattaca aagcaagaaa ttataagcga actgatattt attatcattt
    21061 gaaataaatt taacttaagc gacaatcatt tgtgacgttc attgcagaaa ataaaaataa
    21121 cattatcgct ataaattaaa atacataccg aaattcgtat aatttcagca gttgttattt
    21181 agaatgatta actggaccgc aactgaagca agaatatcag gatgaagctt accgcctcat
    21241 cctgaatggt cattttacca gtgcgttaat gacggtttcc atcgcctgct tttccagctc
    21301 actgcgctga atgcgtttat tggctaatgc tgtacgtaat acgcctgcga taacaatatg
    21361 ctttggttcc tgccccagat cgcgcatttc cagcacaact ttaccgacaa ctctgcacat
    21421 ttctcggtac agttcatcct cttttgtccg atttcccatt ctttatacct atttatcatg
    21481 ccaa
//
LOCUS       DAJTRH010000064        21258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTRH010000064 DAJTRH010000000
VERSION     DAJTRH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            18..791
                     /locus_tag="OO339_002610"
                     /pseudo
     CDS             18..791
                     /locus_tag="OO339_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO339_002611"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO339_002611"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5927195.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO339_002612"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO339_002612"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5927196.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO339_002613"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO339_002613"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5927197.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="OO339_002614"
     CDS             complement(4989..7223)
                     /locus_tag="OO339_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5927198.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO339_002615"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO339_002615"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5927199.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="OO339_002616"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="OO339_002616"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5927200.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO339_002617"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO339_002617"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5927201.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="OO339_002618"
     CDS             complement(12978..14318)
                     /locus_tag="OO339_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5927202.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO339_002619"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO339_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5927203.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="OO339_002620"
     CDS             complement(16106..16555)
                     /locus_tag="OO339_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5927204.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO339_002621"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO339_002621"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5927205.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="OO339_002622"
     CDS             complement(17355..17684)
                     /locus_tag="OO339_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5927206.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO339_002623"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO339_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5927207.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="OO339_002624"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="OO339_002624"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5927208.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="OO339_002625"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="OO339_002625"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5927209.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5228 a         5674 c         5069 g         5287 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 tcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccatcatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcccccgg
//
LOCUS       DAJTRH010000065        20704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTRH010000065 DAJTRH010000000
VERSION     DAJTRH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO339_002626"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO339_002626"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5927210.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO339_002627"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO339_002627"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5927211.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OO339_002628"
     CDS             complement(3121..3420)
                     /locus_tag="OO339_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5927212.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OO339_002629"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OO339_002629"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5927213.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OO339_002630"
     CDS             complement(4632..5069)
                     /locus_tag="OO339_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5927214.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OO339_002631"
     CDS             complement(5115..5456)
                     /locus_tag="OO339_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927215.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO339_002632"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO339_002632"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5927216.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO339_002633"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO339_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OO339_002634"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OO339_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5927217.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OO339_002635"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OO339_002635"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5927218.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OO339_002636"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OO339_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5927219.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13217
                     /locus_tag="OO339_002637"
     CDS             12687..13217
                     /locus_tag="OO339_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5927220.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13329..13865
                     /locus_tag="OO339_002638"
     CDS             13329..13865
                     /locus_tag="OO339_002638"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5927221.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13973..14797)
                     /locus_tag="OO339_002639"
     CDS             complement(13973..14797)
                     /locus_tag="OO339_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5927222.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="OO339_002640"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="OO339_002640"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5927223.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="OO339_002641"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="OO339_002641"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5927224.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="OO339_002642"
     CDS             complement(18729..19202)
                     /locus_tag="OO339_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5927225.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO339_002643"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO339_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5927226.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..>20704
                     /locus_tag="OO339_002644"
     CDS             20352..>20704
                     /locus_tag="OO339_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HCR5927227.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHW"
BASE COUNT         5220 a         5269 c         5062 g         5153 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggat
//
LOCUS       DAJTRH010000066        20612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTRH010000066 DAJTRH010000000
VERSION     DAJTRH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            96..395
                     /locus_tag="OO339_002645"
     CDS             96..395
                     /locus_tag="OO339_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5927228.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="OO339_002646"
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="OO339_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5927229.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO339_002647"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO339_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5927230.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2048..2497
                     /gene="alaE"
                     /locus_tag="OO339_002648"
     CDS             2048..2497
                     /gene="alaE"
                     /locus_tag="OO339_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5927231.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2534..2878)
                     /locus_tag="OO339_002649"
     CDS             complement(2534..2878)
                     /locus_tag="OO339_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5927232.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3030..3260
                     /locus_tag="OO339_002650"
     CDS             3030..3260
                     /locus_tag="OO339_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927233.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3607..3852
                     /gene="nrdH"
                     /locus_tag="OO339_002651"
     CDS             3607..3852
                     /gene="nrdH"
                     /locus_tag="OO339_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5927234.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3849..4259
                     /gene="nrdI"
                     /locus_tag="OO339_002652"
     CDS             3849..4259
                     /gene="nrdI"
                     /locus_tag="OO339_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5927235.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4232..6376
                     /gene="nrdE"
                     /locus_tag="OO339_002653"
     CDS             4232..6376
                     /gene="nrdE"
                     /locus_tag="OO339_002653"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5927236.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6386..7345
                     /gene="nrdF"
                     /locus_tag="OO339_002654"
     CDS             6386..7345
                     /gene="nrdF"
                     /locus_tag="OO339_002654"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5927237.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="OO339_002655"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="OO339_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5927238.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="OO339_002656"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="OO339_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5927239.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="OO339_002657"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="OO339_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5927240.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="OO339_002658"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="OO339_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO339_002659"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO339_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5927241.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="OO339_002660"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="OO339_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5927242.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="OO339_002661"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="OO339_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5927243.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="OO339_002662"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="OO339_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="OO339_002663"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="OO339_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5927244.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO339_002664"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO339_002664"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5927245.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO339_002665"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO339_002665"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5927246.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="OO339_002666"
     CDS             complement(19399..19827)
                     /locus_tag="OO339_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5927247.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO339_002667"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO339_002667"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5927248.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4974 a         5273 c         5148 g         5217 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat
      781 taaacggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc agaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat gcaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa
     1861 tccacaatgt acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat
     1921 aaaaattttt cactaattga ttagtcatag acagcgatat acgctatgca aaaatgcaga
     1981 tggcaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
     2041 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
     2101 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
     2161 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
     2221 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
     2281 gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
     2341 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
     2401 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
     2461 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
     2521 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
     2581 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     2641 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg
     2701 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     2761 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     2821 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     2881 ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     2941 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     3001 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     3061 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     3121 tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     3181 aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     3241 cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg
     3301 gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt
     3361 gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt
     3421 gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat
     3481 cactatttgc tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta
     3541 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     3601 cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa
     3661 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     3721 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     3781 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     3841 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     3901 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     3961 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     4021 tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     4081 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     4141 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     4201 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     4261 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg
     4321 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg
     4381 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     4441 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     4501 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     4561 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     4621 cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga
     4681 tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca
     4741 gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat
     4801 tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt
     4861 gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     4921 cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc
     4981 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     5041 cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     5101 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     5161 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca
     5221 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     5281 cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     5341 cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga
     5401 aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     5461 ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     5521 tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     5581 tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg
     5641 gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc
     5701 cctggatttc accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat
     5761 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag
     5821 tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     5881 cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     5941 cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg
     6001 gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     6061 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     6121 aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta
     6181 cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac
     6241 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     6301 gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     6361 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     6421 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     6481 accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga
     6541 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     6601 tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc
     6661 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     6721 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     6781 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     6841 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     6901 ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga
     6961 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     7021 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     7081 cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     7141 gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc
     7201 cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     7261 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     7321 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca
     7381 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact
     7441 gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat
     7501 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg
     7561 cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca
     7621 gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaagaaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tgagctacgg at
//
LOCUS       DAJTRH010000067        20544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTRH010000067 DAJTRH010000000
VERSION     DAJTRH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="OO339_002668"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="OO339_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5927249.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="OO339_002669"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="OO339_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5927250.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="OO339_002670"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="OO339_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5927251.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="OO339_002671"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="OO339_002671"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5927252.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="OO339_002672"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="OO339_002672"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5927253.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="OO339_002673"
     CDS             4451..4816
                     /locus_tag="OO339_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5927254.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="OO339_002674"
     CDS             4966..5484
                     /locus_tag="OO339_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5927255.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="OO339_002675"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="OO339_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="OO339_002676"
     CDS             6715..7197
                     /locus_tag="OO339_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5927256.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="OO339_002677"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="OO339_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5927257.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="OO339_002678"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="OO339_002678"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5927258.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="OO339_002679"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="OO339_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5927259.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="OO339_002680"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="OO339_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5927260.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="OO339_002681"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="OO339_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5927261.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="OO339_002682"
     CDS             10941..11732
                     /locus_tag="OO339_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5927262.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="OO339_002683"
     CDS             11753..13039
                     /locus_tag="OO339_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5927263.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="OO339_002684"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="OO339_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5927264.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="OO339_002685"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="OO339_002685"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5927265.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="OO339_002686"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="OO339_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5927266.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="OO339_002687"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="OO339_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5927267.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="OO339_002688"
     CDS             complement(16987..17277)
                     /locus_tag="OO339_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5927268.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="OO339_002689"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="OO339_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5927269.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="OO339_002690"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="OO339_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5927270.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="OO339_002691"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="OO339_002691"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="OO339_002692"
     CDS             19139..20380
                     /locus_tag="OO339_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5927271.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5305 a         5365 c         4819 g         5055 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatttgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacatcccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattgcaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aagaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtt ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gttcggtaat gctgccaact tact
//
LOCUS       DAJTRH010000068        20397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTRH010000068 DAJTRH010000000
VERSION     DAJTRH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..4225)
                     /gene="mukB"
                     /locus_tag="OO339_002693"
     CDS             complement(<1..4225)
                     /gene="mukB"
                     /locus_tag="OO339_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5927272.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDGA"
     gene            complement(4225..4929)
                     /gene="mukE"
                     /locus_tag="OO339_002694"
     CDS             complement(4225..4929)
                     /gene="mukE"
                     /locus_tag="OO339_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5927273.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4910..6232)
                     /gene="mukF"
                     /locus_tag="OO339_002695"
     CDS             complement(4910..6232)
                     /gene="mukF"
                     /locus_tag="OO339_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5927274.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6229..7014)
                     /gene="cmoM"
                     /locus_tag="OO339_002696"
     CDS             complement(6229..7014)
                     /gene="cmoM"
                     /locus_tag="OO339_002696"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5927275.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7150..7929
                     /gene="elyC"
                     /locus_tag="OO339_002697"
     CDS             7150..7929
                     /gene="elyC"
                     /locus_tag="OO339_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5927276.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7906..8799)
                     /locus_tag="OO339_002698"
     CDS             complement(7906..8799)
                     /locus_tag="OO339_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5927277.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8953..9699)
                     /gene="kdsB"
                     /locus_tag="OO339_002699"
     CDS             complement(8953..9699)
                     /gene="kdsB"
                     /locus_tag="OO339_002699"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5927278.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9696..9878)
                     /gene="ycaR"
                     /locus_tag="OO339_002700"
     CDS             complement(9696..9878)
                     /gene="ycaR"
                     /locus_tag="OO339_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5927279.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9930..11162)
                     /locus_tag="OO339_002701"
     CDS             complement(9930..11162)
                     /locus_tag="OO339_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5927280.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11199..12185)
                     /gene="lpxK"
                     /locus_tag="OO339_002702"
     CDS             complement(11199..12185)
                     /gene="lpxK"
                     /locus_tag="OO339_002702"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5927281.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12182..13930)
                     /gene="msbA"
                     /locus_tag="OO339_002703"
     CDS             complement(12182..13930)
                     /gene="msbA"
                     /locus_tag="OO339_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5927282.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(13967..16226)
                     /locus_tag="OO339_002704"
                     /pseudo
     CDS             complement(13967..16226)
                     /locus_tag="OO339_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16433..16717)
                     /gene="ihfB"
                     /locus_tag="OO339_002705"
     CDS             complement(16433..16717)
                     /gene="ihfB"
                     /locus_tag="OO339_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5927283.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16877..18550)
                     /gene="rpsA"
                     /locus_tag="OO339_002706"
     CDS             complement(16877..18550)
                     /gene="rpsA"
                     /locus_tag="OO339_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5927284.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18661..19344)
                     /gene="cmk"
                     /locus_tag="OO339_002707"
     CDS             complement(18661..19344)
                     /gene="cmk"
                     /locus_tag="OO339_002707"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5927285.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19517..20281)
                     /gene="ycaL"
                     /locus_tag="OO339_002708"
     CDS             complement(19517..20281)
                     /gene="ycaL"
                     /locus_tag="OO339_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5927286.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4910 a         5656 c         4945 g         4886 t
ORIGIN      
        1 ctgctccatc gaggaacagc aggcggcaag gagagatatc tttaccgcgc aggcggcgag
       61 attcatcttc ccagctttgt accaccatca ccagaatcga cataccggta ccaatcgcct
      121 caccggtcga caatgcacca gactctgcgc gcaaccaacc atcggaacca cggttaacct
      181 caacttccat ttccagatag ttgcggtaat ccagcagttc ttcgccaatg gtctgcggcg
      241 tgcgctgccc catatcaatc tgcggattaa gacgctgata caatttcgcc aacgcttccg
      301 agaaggtcag acggttgctg ttaaacagat cctgatgctg ctcgtgctgt tcagagagca
      361 catccagcag catggcgtgc gtttcacgca cgttcacgtt gagacgcacg ctgttcacct
      421 gaccaaacga tacgttctgc aacccctggt tgagcatacg gatacggttc tgctcgcgct
      481 gaatggtttt gcgaatgatg ttcgccacgc tgcgggaact gatcgccagt ttctgttcac
      541 gggaggttaa ttcttcggtc agacggctaa gttcaatctc catctgttcg atagcttcca
      601 ccggatcatc ggtacgaata atatcctgac gaatacgttc acgcagatgc tgataaaccg
      661 ccacgaagaa ctgaatttta cgctccggac gtttcggatc ttccgacatg cgcagcacgt
      721 cgcgcagatg ttcgttatcc gccaccgcca gacgcagcgc acctaacgcc ttatccgaca
      781 tggaacgcaa atcatcagcg gagagataag ccagctcacg acggtgtaag cggcgctcaa
      841 cgccgttatc tttcaccatg cgcatcaccg cacaccagcc cgctttggcg gtcactacct
      901 gctcgcgcat ctcaaagtaa tcccgctcca gcttgcgcag tttgcgggtc aggttgtcca
      961 tctccgcttc gcagaaggta agcgcttttt ccagttgatt gcggcgtgaa cggttattgc
     1021 tcagttgcgc atgcagctcg tcacggcgaa tacgcgcccg ctcttctgcc ccgctatcag
     1081 cacgcacgcc gatatcctgc aattcacgtt gcagatcgtt gagtagctct tttttggtgt
     1141 cgtaagaact tttcagcgaa gccagcacct ggttgtactg actcaactgc gctgcgtgac
     1201 cgcgcaacgc ttcgcgagcg cgggtacgct ccgcttccgc ctgttccaga cgttcacgca
     1261 gtttttcgtt gagatcgctg ttaccgctaa gcatttctgc cgagtcagaa tagctaaagt
     1321 gcgcacgacg ctgcaccact tccgtcaggg caaacgcttg ctgacgggca tcgcgctgca
     1381 tctgctgaga gtacgcgtaa tcttctttta actgttcgaa ctgttccggg tcgctctgca
     1441 ataccgaaac gatcggttcc agtttcgcca gttgattgcc aaactgctga acaaaacgcg
     1501 cggcttcctg ggcttcatcc agacgttcgc ggatttcatc gacacgatcc gccaggctgt
     1561 catcagccaa caggttgaga cgcggcagaa tgcggttcag cgccgtaacg ccttctttcg
     1621 cctgctcaaa ctgaatacgc tgctgctggt tatcattttc atgattactc aacgcccgct
     1681 ccagttcgac tcgacggctg ttcagttgac ggatttctgc ttccgggtca gactcaaacg
     1741 ccaccgccag atgactgccg ataaagcggc tgaacgcctg atgcagacgc tgggttttct
     1801 gtacatcaaa ggagagcgtg gcgaagcgtt cggaaagcac ttcacgctcg gcatggaggc
     1861 tttcaatacg gctttcacgc gcagcacgac caaacagcgg cacttccggg aaacgtgaat
     1921 aacgccactg tcgatcggcg attttcacca ccaccgcttt ttccagctca tcaacgctga
     1981 acacgctatc atcgaatgac tgcggatccc cttcgatcaa atagagatct tccgggcaat
     2041 cggtcaagcc ttccaggtgt tcggttacct gtgacagatc tggcaccacg atggcgtggc
     2101 gtgacgggcc atacagagcc gagaagtacg gcgcatcttc caggctaacg tcgtcataaa
     2161 tttctgacag cagcacacca ccaaaacgct ccgccagcgc gttcagacgc tgatcttcag
     2221 agccgccagg ctggcttaaa cgttcgatct cttcatcgac ggcgtttttg cgcgcgccta
     2281 cttcatcgcg ttcaacaatc gcctcgcgct cacgctccag caactgttgc agatattcag
     2341 tgacgtcctg gctggaggag aactcttcgc cgcactgttc actcaactgg ttgagactgt
     2401 tttgcgctgc cagccaaacc ggtgcacgct gcatcaaact ctgaatgcga gactgcagct
     2461 gttcctgttc ctgccgcagt gccatccgct cttcacgggc gttagatacg ctatcggaaa
     2521 gagaggcaat gcgtgcttcc agctcctgat gcagagcttc cagttcatca atatcaaaat
     2581 tcttgccctg acgtttgcag aaatctgcca gcagacgctc agcttcttgc tgctcacgca
     2641 gacgctgttc cagttcgctt aagcgcatcc gcaacggctg aacctgctct gccaggtgac
     2701 gctgatcaac cccttcgcgc aatagttcgc gagcgacatc ccacgcctcg ttacgcgcca
     2761 gcgggccgtt gattgccacc accagctgat aagcctgctc aaactggctg tgcgcggttt
     2821 gcgccatgct cattttctgc tcaagagaga gcattttttc agtcgcttcc agctctttcg
     2881 cctggaaggt ttccagccat tcggcggcgc tgtcggcggt taagtccggc agatggcaca
     2941 gttctttggc acgattaagc gcagcaatcg cctggttata ctggatcgcg cgcgtttgct
     3001 ggacgtccag cgcctgctgg tagtcagcaa gctggctttt cagctcgtcc acttccagtt
     3061 cggcagcttc cgcgcgagcc tcattctctt cctggcgttc gatggcttct gccaccactt
     3121 cattttgctc ttccagacgg atctgcagct catcgagatc cacttcgtag cgttcgatct
     3181 tctcctgctg acgcaatgcg gtttgcacca ggttcaggtg atcgctggcc gcctgataat
     3241 ccgcttccag atcaccttcg gcaccgttgt gctctgccag ctcacgcgcc atatcgacgt
     3301 gtttgtactg ctcagccgcc agttgctgac gcgaagtatg tagctcgcga cgaaactcca
     3361 gggctttgtc gagatggaca cggcgctcgt tggcgtgacg catgtagtcc gccgccacgt
     3421 agttggtagc ttcgctgatc agatgcttaa acaggtcacg gtccgactgg gtgacacgaa
     3481 tcgcttccag cgtcatgcgg ttttcacgca gcgccgcttc catgtcctgg aacgctttac
     3541 gcacgccgct gttttccggc aacaggtagt cgcgcagaga acgggtaatg gcactggaga
     3601 tcccgccata cagcgaagct tcgatcagac gatagaattt gctacggtca gatgcggagc
     3661 gcagacgacg cgcgatgatg cccagatcga acatcagcga gtggtaatcg gtaatggagt
     3721 tgaactgttt aaactgcacg ccttccatcg cctcgagctt gtctttcagc tcgttaagcg
     3781 gcagcacgcg cgcctggcgt tcgttcaggg tttcggtcac cagctgtgtc ggctgcaccg
     3841 acatcggcag tccctgaatg gcaaatggtt tgatatccac tttacgatcg cgtccggcaa
     3901 cctgttgcag acgcacaccg accaccacgc gctggtggcg cgagttaatg gtgtcgagca
     3961 tcgaataaca gacacccgct ttcagcttac cgtgcagacc tttatcgcgc gaaccgctgg
     4021 tggccccggc ttccgtagtg ttacggaaat gcagcagggt caggtcgggg atcagcgccg
     4081 taacgaacgc cgccatcgtg gtggatttac ccgccccgtt accgccggaa agcgtcgtga
     4141 ccagctcgtc aaggtcaaaa gttcgggcaa aaaagccgtt ccagttaatc agcgtcagtg
     4201 agcgaaattt accgcgttca atcattattc ttcctctccg ctatctggct gactctcttc
     4261 ggtttcatcg ttgagttgca gatgattttc aatcggcatt gcttcgccat cacgaatcag
     4321 gcgacgctgt gcttcacggg gatcgtcgcc agcacgcaca tcggctccga agcggaacac
     4381 cgattcggta atgcggaact tgctgctgtc gtggcccata aaccacacca tgcctaaacg
     4441 acgcagacgg ttgagcgaag aacgtacttt ctcctgcaac ttctgacggt caacgtctga
     4501 accggttgaa cggttgttca ccagtttcag cagtttcgct tcatcggcca gggtgagcag
     4561 ttcgtcgtac agttcctgct gggtgaaaat cccctcattc gccagccgtt ccgggctgag
     4621 atagagataa cagaggattt tcccgaccat catatccagt tccgacaaga cggaacgcgg
     4681 gataagagtg gtggaacgtg ggcgtaaata gaagaaccct tccggtgcgc gaataagctc
     4741 aacgttataa cgcgcgtaaa actcttccag atattcctga aaatccatca ggaatgcatg
     4801 attatccagt tcgtcgaggc caatatggcg gcctgaacgt aaggcgctgt ccagcgccgg
     4861 aaataacgga ttcgccagcg cctgcgccag cttaaccggc atcacttgtt caatatttgt
     4921 cgatgacatg cgcctgtacc ttggctccgt aatcattaat cggctgccat ttcgctggca
     4981 gtccggtgaa atctgcttgc gctacgccaa gacgtaccgc ctgatcaata acaatacgcg
     5041 caacgtcaaa gtgacgtgca cgcggatact gtgacagata ttcgcgtacc accagaccaa
     5101 gatccagcgg cacttgtctg gttttgtaca cggcaagttg ttcttcgatg atcgccgcca
     5161 gctgttcgcg gatctcgtta aactcttcgt attccagatc ctcaggaagt tccccagtca
     5221 cttcttcatc gcgcagtgcc atctcttcgt cacgcatatc cagcagacga tcggcattgg
     5281 cataagttag cgcccacggc tcatcaaaat aggtttgtac cgactgacgt aaccgctgag
     5341 caaagacgcg gtttttatcc atatcgatcg cggtacgaat aaatttgtgt acgtggcggt
     5401 cgtagccaat ccacaagtcg atggattgct ggccccaact gataatacga tcgagtttgc
     5461 tctgcagatc gaacaccaga cgatcgacga aatgcagatc gtcatgggtc atcgtcgcat
     5521 cctgaatgcg caacagatta gcctgcaatt tgtcgcctgc cgcttccagc gtatcctgca
     5581 attcacgcag cgttccggaa gtttccgaaa gcaacaattc acagctggaa atcgccgccc
     5641 gccagtcttt gttcagcaac tgggcgatat cgtccttcac ctgctgctgc tgttcgtcca
     5701 tcagacgttg cgtcaggtcg atactgtcga aaatttctgc taccgaatat ttcagtggcg
     5761 catagacatt acggtgccag tgaaattcat caccgccctc ttcggcggca tctgctgcgc
     5821 gtttgagctc acccgccaca atcgacaact gcatagaaag acgcagcgta gaaaactcgc
     5881 gctgacggat gtagtagtca gtaatgccga tgccgagcgg cgtcagacgg taaattgcgt
     5941 tcccttccgc ctgctcgctg gtaaagcggt tcagcagacg ttgacgcacc atgtcgttga
     6001 tcgcgttatt ggcgcgcacg ccgatggttt cgctggtttg ctcaaacgca tcactcacat
     6061 ggcggaatgc atccaccagc tcgccttcac tcatctcacc atccagacgc tcgccgttca
     6121 gcgtggcaac cgccagcaga aaagagagtc ggtctaccgg cagcgagatg gagaagtcat
     6181 tttttctggc ccaggcaacc agttcgggga ctgtctggga aaattcactc atactttatc
     6241 cttgctctgc ggtttgcgcg cggtgacatg aatataacgc cctagggtaa tatacggttc
     6301 ctgacggcaa taacgcgttt ctaattcaag taatgcttca tagcagtcgc gctgctggta
     6361 cttctcgcgc agataatcat gaaacacgcg aacgcctgtc ttacccataa tttgccaacc
     6421 agcttcttcc agccacagat aaacctgcgc cgggtcgcgt ggataatctg gcgaaagcgt
     6481 ccgttttttc tttttcggca ttcccgcctg cacgtaatca aaattcccgg cgaccatgtt
     6541 atgcatcaac aaaccatgcg cattgtagaa cattaacgac aacactccgc ctggacgcaa
     6601 cactgaccac agggtctgca atacactgcg gggatcggcc acccactcga gcaccgcatg
     6661 gaacaatatc agatcaacgg gcgtttccaa atgcgaagca acatcctgag cggcgcaatg
     6721 tataaattgc atgttgtcgc tcacaccttt tgcttctgcc gcctgttttg cgcggtcgat
     6781 catctgcgca gaaagatcgc ataaaataac ctgatgcccg cgctcggcca ttttgattgc
     6841 ggtctgccct tctccgccgc cagcatccag cacacgcagt ttttgcgggc ccatttccgc
     6901 cagcacgcga tcgagatcct gccacagaat agcctgtcga agctgccctt tggtggtgcc
     6961 gtaaatgtta cgggaaaact tttccgcaat atcatcaaaa ttgcgatcct gcattgcttt
     7021 ctccaccggt tggctaagac gttttctgat aaagacgcta ttttgtcaca gccacggcga
     7081 gaatgaaccc gtctggtgta atatcgctag cgatcccttg ctatatggtt aaaaaaggaa
     7141 ccagaaagga tgctttttac actgaaaaaa gtgattggca atatgttgct gccacttccg
     7201 ctgatgttgt taattattgg cgctggcctg gcgctgctgt ggtttagccg ttttcaaaaa
     7261 accgggaaga tttttattag cattgggtgg ctggcactct tgttattgag tttgcaaccg
     7321 gtagccgacc gcctgctgcg tcccatcgaa agcacctatc cgacatggaa taattcgcaa
     7381 aaagtggact atatcgtggt gctcggaggt ggttatacct ggaacccgca gtgggcaccg
     7441 agctctaatt taatcaataa cagtctgcca cgcctgaatg agggtatccg tttatggcgc
     7501 gaaaatccgg gttcaaaact gatcttcacg ggaggcgtag caaaaaccaa tacggtgagt
     7561 acagcggaag taggtgccag agttgcgcaa tcgctgggcg taccgcgcga gcaaattatc
     7621 accctggatt tgccaaaaga taccgaagaa gaagctgcgg cagtgaaaca ggcgattggt
     7681 gacgcccctt tcctgctggt gacctccgca tcacatctgc cgcgggcgat gatctttttt
     7741 cagcaggaag ggttaaatcc gctaccagcc ccggcgaatc agttggccat cgactcgccg
     7801 cttaatccgt gggaacgggc aatcccttct ccggtctggc tgatgcatag cgatcgtgtt
     7861 ggctatgaaa cgcttggccg catctggcag tggctgaaag gtccgtcagg cgagccacgg
     7921 caagagtgat tttgatgcca gatcgaagtt gcgccgacta aatcgtccgg tattaaccag
     7981 ttgcgcaact tcatcccata acacatacaa ccaacgccgc cagatgaacg actccgccac
     8041 tggcgcgcgt tgcaggtaac tccagagcaa atcttccgcc agagaattat ccattagtcg
     8101 gaacagttcg tattctcgcg gtgcccaaag cattagtccc ggcccgacca tcgccagtaa
     8161 ctgatcgctg cgcgaatctt tcagcatgct gcgtaaacag aagttaccgt gaatcaacac
     8221 acaattgtcg ttaaagcctt caaataatgc cgggagacat tcgcgagtgc gaaacaggat
     8281 ccgcttatcc tgcatcgtca gaccggtgtt attgaactga ttgagcgtgg tccatagcac
     8341 ttcaacatgt tgccggtacc atgagggcca gaaattttcc tgagtattgt cgaccgcgcc
     8401 gacgcaaccg cgactgtcct gacggtgcca ggccagtaag gcttcaacga tttggtcttt
     8461 gagctgttcc cagcgttctg gtgttcgggc tggtgcctcc accgaaacac cacgcatccg
     8521 ctccagtagc aggacatcag ggccggggtg ttcttcatgc gtcatcacac cataaatagt
     8581 cggcatacgg acagtcccac ttcttgccag catggtggtt ttccatgcca gttgtcgggc
     8641 ttttccgggc gtactaaagc ttcttgccat taacggcatt gggtttccct ggctgtcata
     8701 caaagcccac aacgccgtat ccgctttttc attgacgcac tcaatacgac tgagtttttc
     8761 gcccagtaaa tggcttaatt cggcacgcag ctgttccatt cagattaccc tcataaaagc
     8821 tactgctttc ataatgagcg ccaatataaa agatgtcacc tgaatgagat caattgttga
     8881 aaagaaaaat tgaggaaaat cgtaacggta cgacactcct cccaaaattg gctgaagtgt
     8941 cgtgaagtga aattagcgca tttcagcgcg aacgcgctca agatcttcag gggtatccac
     9001 acctgtgcca ggaacttcct gagcaacagc aacatggatt ttttcgccgt accacagaac
     9061 acgaagctgc tctaacattt cgatgtgttc taacggactt gcctgccagt tgacgtaacg
     9121 acggataaag cctgcacggt agccataaat accaagatga cgcaggaagt tatcgccaac
     9181 ggtttcaagg ccttctgcaa aacgatcacg atcccaagga atggtggcac gtgagaagta
     9241 cagtgcatac ccttcagcgt cgagaaccac tttcaccgca ttcgggttaa acgcttcttc
     9301 cgcattgtgg attggcaccg ccagagtcgc catacccacc tgacgctgag cgaggttatc
     9361 agcaacctga cgaatgattg tcgcagggat catcggttca tcaccctgca cattaacgat
     9421 caccgtgtcg tcgctgaatg cgcatttttc gacaacttcc gccagacgtt ctgttcctga
     9481 ctgatgatcg gcgcgcgtca tacatacttc accgccagcg gcttcaacgg cgcgggcaac
     9541 atcatcatga tcggttgcca cgatgatgcg ctcggcacct gattcacgcg cgcgttcaag
     9601 aacatgaaca atcatgggtt tgccgttaat atcaaccaat ggtttaccgg gcagacgcgt
     9661 ggacgcgtag cgcgcgggaa taatgaccac aaaactcatg atttactctc atcagcagtc
     9721 agcacgcggg cttccgtttc cagcaacact ggaatgccat cgcggagcgg gaaagccagg
     9781 ttatccagtt tgcaaataag ctcttgtttt tcctggttat accaaagttt tccgttgcaa
     9841 accgggcagg caatgatttc aagcaaacga tgatccatag ttcctccaga tggatcgggt
     9901 tatgaatgca taaatcttat cataatcatt tatgcgacgg ggtctatttc ccagccagtg
     9961 cggcaatcag taaagagacc ttgcgggcag cgtcctaatg tcacccgcgt tgcctgctgc
    10021 cagctcgcga aatcagtaat cgcctgacgt aacccttttt gcagcattgt cgttggttta
    10081 atgccttcct gtaaccacag agagataact tcaaggatgc ctgtctggcg atgcattttg
    10141 gcatccattc gcccaactaa ttgcccacga tgtaataatg gcagaacaaa atagccatac
    10201 tggcgtttcg gcgctggggt atagcactcc agccggtagc taaaatcaaa aagctgctct
    10261 gcgcgtttgc gatcccagac aacaggatca aaaggcgaaa gtaccgcgct gtgcgttgca
    10321 gtgagctttc cggccagcgc acgctcgagt agcggcagca aatcagcatg cagccaaaga
    10381 ttgcccaatt tttcaacatg cacagcaatg atttgctgct gttcagccct cgcttcgcgc
    10441 cacgccgcca gtgccggacg tttcagccga tagtaatctg ccagccactg ttcgcggaat
    10501 attcccaggc tacgcgcact gttatccagc atgatgattt ctgcttctgt ttgcgaaacg
    10561 agatcgcgct catcatccca gtcaggcatg acacggtggg ttaaatcata aacgcgctgg
    10621 aagttgcgcc gttcaatcac catcaccttt ccggcagtaa ataaaccttc cagatgccgt
    10681 ttatgcggct tccattccca ccagccgctt gcacctttac gaggatgctc aaaatcggct
    10741 gaacgtaccg gccctttatc atgaatatgc tgaattaact gtgcaatttc cgcctcatgt
    10801 tcctgcatcc aggcgtcttt gtatttccag cccatttttt caggtgccag catgcggtgg
    10861 cgaataagac gaaagtcgct acgcggcata aagcaggctt catgcgccca gtattccatt
    10921 aattcgccac gcgccagaga ctcatccagc cactgggcag gataatttcc cagacgactg
    10981 aaaagcacca gatatggact acgggcaaca atattgatgg tatcgatttg cagcaaggac
    11041 atgcgggaga tcgttgccgg aatatcctcc aacgacgctc gacggcgggg tttgtttaac
    11101 aggccttgtg cggcaaggtg aagattacgc gcatcagcaa gggagaggtg cggcagcgac
    11161 attcatgact ccatcaatcg aacgctgccg cggcgtaact agttgccaga agccagcgag
    11221 gttagttgcg taagcagttt cgctggttca tcacctgaaa gctgtgcgtc tacaggcaaa
    11281 taccaccaat tttcttctgc aaaggcccgg catttcaccg catctttttc agtcattacc
    11341 agcgtttgcc cggcgcttac caacgcactg acatcctcat ggttcaaaga ctgatgatcg
    11401 gccagcggta cacatttttc cggttgtacg ccacacatct tcagcgtggc aaaaaagcgc
    11461 ggcggatgcc caatccccgc catcgccact acatgttcaa gctgagcaac gtcacaacgc
    11521 gtaccggtac gtaaattcac cgcctgaccc ggcagcagat gcatggggat ttcaccgctg
    11581 cgagggacac cgccgttgac gattaccgca tcaaccgact ttaagcgccc cgctcgctca
    11641 cgcattggcc ccgccggcaa ccaccagcca ttgccaaagc gacgcacacc atcaataacg
    11701 acaatttcca catcacgcgc cagacggtaa tgctgtaaac cgtcgtcggt tacgatgatc
    11761 tgcacatcag ggtgttgcgc cagaatggct tttaccgcat cagaacgaac gggagaaacc
    11821 gcaacaggcg catcagtgcg ttgataaatc aacacaggtt catcacccgc ctgtgctgtg
    11881 gtggtatctg ccgacaataa cagcggataa gattcagcct taccaccata tccccgcgat
    11941 acgaccccca cgcgaatacc gcgctgttgc aactgttcca ccagccagac aacgaccggg
    12001 gtttttccgt tgccgcctgc ggtgagatta ccaaccacga caaccggtac gggggcacgc
    12061 caggcgcgct tcagttttag tttatagcaa agacggatcg cgccactcac caggccatac
    12121 aaccaggaga gtggcagcaa tagccgccac aaaggggatt caccagacca gattttttcg
    12181 atcattggcc aaactgcatt ttgtgaagtt gcgcgtaaac gccgcggtgc tcaagcaaat
    12241 cgttatgcgt accgcgttcc acaatgacac catcctcgac gaccacgatt tcgtctgcct
    12301 tttcaatggt agacaagcgg tgggcaatca ccagagaggt acggtttttc tgcaactcat
    12361 ccagtgccgc ctgaatcgca cgttcggatt cggtatccag agccgaggta gcttcatcca
    12421 gaatcagaat cgggctatca cgcagcaagg ctcgagcgat agcaatacgc tgacgctgac
    12481 cgccagagag cagcacgccg ttttcaccaa tcactgtatc gagaccgtta tccatcttat
    12541 tgatgaagtc catggcgtag gccatccgcg ccgcttcttc aatttgctca cggctgtact
    12601 gttcagtccg tgcgtaagca atgttgttag caaccgtatc gttaaacaga tggacattct
    12661 gcgacaccag agcaacctgg ttacgtaacg acgccagggt atactcgcgc agatcgtgac
    12721 catccatcag gatttcgcct tcatcaatat cgtaaaaacg cgtgatcagg ctggcgatgg
    12781 ttgatttacc tgaaccagag cgtccaacca gagcaaccgt cttccctgcc ggaattttca
    12841 ggttgatgtt acgcaatgcg ggtacgtcac gtcccggata agtaaaggtg acattgcgga
    12901 attccacgtc gccagtcgca cgctcgatca cgcgcttacc ttcgtctttc tcctgctcac
    12961 tgtccagaat ggtaaacagc gtctgacaag ccgccatacc gcgctggaac tgggcgttaa
    13021 cgttggtcag cgatttcagc ggacgcatca gtgcaatcat tgaagagaaa acaacggtaa
    13081 tcgtaccggc agtcaggtta tccataacac ttgggaagct cgccgcatac agaacaaacg
    13141 ccagcgccaa agaggcgatc agctgaatga tcggatcaga gatggaagag gctgaaacca
    13201 ttttcatccc ctgaagacgc attcggttac tgactttatc gaagcgtttc gtttccactt
    13261 cctgaccacc gaaaatcaat acttctttgt ggcccttcag catttgttct gcactggtgg
    13321 tcacttgccc catggtgttc tgcatgtttt tactgatgtt gcgaaaacgc ttcgatacta
    13381 cgcgaatcgc aatcgaaaca atcggtgcca gcacaatcaa aatgatcgac agttgccaac
    13441 tgtaatagaa catcatgatg aacaggccga tgatcgacgc accttcacgc acaacagtaa
    13501 tcagtgcgcc ggaagaagaa gaagcaacct gttcggaatc gtaggtgata cgtgacaaca
    13561 gcgtacccgt tgactgtttg tcaaagaatg aaactggcat tcccatcatg tgaccaaaca
    13621 ggcggcgacg catggtcatt accacctttc ctgataccca ggagatacag tagctggaga
    13681 catagctggt gataccacgt aaaatcatca gtccgatcac caccagcggc atccacacca
    13741 gcacggagcg atctgtttta ccaaagccat catcaagaag tggcttaagg agcgataaca
    13801 tgaaggtatc gctggctgcg ttgaggatta acgctacgcc cgccacgatc agacccgctt
    13861 tgaaaggcgc aatggttggc cacagtcggc ggaatgtctg ccacgtagag agatctttgt
    13921 cgttatgcat tcaaaaaacc agcatttgtt gaaatagccg catattctac ccgttatcca
    13981 ctggcacgcc aaaccactga tgataccaac gaggtaaaat ttgctctctg aggctgctaa
    14041 tccgccagcc ttgtgctgaa aaattgaccg tcacttgccc ctgatgtgga gtatcaagcc
    14101 attgatatcc tttctgttga tagcgatgct taactttgtt agagggcaat cgccatgcgt
    14161 tatagcgcga tgccgatgcg agtgccactt ttccattcac tcgctgaatt aatggcaacg
    14221 atgatgaggt attactgcca tggtgaggta cctgaagcaa tgttgcctgc acttgctgcc
    14281 agtaacggct tagcatcttt tgttcagctg gggcttcaat atcaccggtt agaagaatgc
    14341 tattcgtccc gtcatcaacc ttaaccacac aggaatggtt atttccctta tcgttgctac
    14401 cttgtaaagg ccagtgcgcg ctgaaacgca atccttgcca ctgccacgct tcgccacgca
    14461 cacagggctg atgatgttcc cagtttaacg gacttctgat ccataacatc ggccatatat
    14521 gcaatattga atccagccct ccccggtgat ccagatgttc atggctcaga ataacgcctt
    14581 ccggttcaag attatgccag tggagccagg ggataatcag ttgttgccca ctatcccctt
    14641 caggccagtc ctgtgtcata gagaatcgct ttgccgtttc tggctatcac cattgccagc
    14701 ccttgcccga catcaagcat gtacagctgc cactcgtcag gtcgaggttt ttgccacagc
    14761 ggccagcaca tcagcaagcc tccagccaca cacattgctg gcaacgttcg ccaggcgttt
    14821 aatcgccata ccaccagtaa gaaccatggg gaaaatgata gccattgcca acattcaaca
    14881 atgttgatcc acccttccgg caaactcttt aacccccaga aaagtaaagc caaagaccgg
    14941 tcggcaagaa accataaccc ctgttccagg attaacggcc cgctaaaatg cacaaccatc
    15001 gcggcgagga tcaacggaac cgtgataaat gtcaccaagg gaattgcaaa tagatttgca
    15061 ataaacgagg tcagactaat gccatgaaat atgacgattt gcatgggcat aagcaggagt
    15121 gtgattccca gttgcagatg gatgagggaa acaactgcac gcaataccgg cggcagttgc
    15181 cactcaggac agggaaacca ctgataccaa aaaatcagtg ccgcgaccgc agcggcagag
    15241 agccataaac tttgcgagag aatggcaaca ggatccatca gcaaaattgc cgccagacaa
    15301 catatccata catcccagcc actccactgt cgcccactta acttaagcat tccccacgta
    15361 gcaagcgcca ccacggtacg caatgcagga ggttgcattc ccgtcaacca ggcataaaaa
    15421 gcagcacagc agattccgcc aattaatggt atttgccagt ggatccagcg cccaggcaga
    15481 aaaatttgtc cactgcgaat gagtccggca gccagcaacg ccgcaaaagc gatgtgcaat
    15541 cccgatatcg ccattaaatg cgccgttcca gtatcgcgca tgatattttt gatttctttg
    15601 gggacggata accgttcccc catacctaaa ccaagaataa ccgcattcca cggatagggt
    15661 tgcagcgttg tttgtagtga cgccagatac tgtgcacgca ggctacaatt cggttcaatg
    15721 acacttgcct gcagaaaacg gccggtgagc ggctgatgct gggcaatggc ataacgctgg
    15781 ctatcaaagc cgccatcatt aagttggcca tgaactgcac gaactttgag tttcattgac
    15841 cattgttgtc cagcacaaac cgcttgcgga agttattcgc catacagcac aaggcctggc
    15901 gcagggaata tacgtcgacc ttgtagataa gtaatttgac cgtaatgagt ggtcatgccg
    15961 tcagttgcag tgatctcaac aattgcatcc tgcgtcgcgc cagttaaggt ttctcctgcc
    16021 cacaaaattt gctttgctga taatatgccc caaacgaaaa acagcaacgt cagcgcgata
    16081 taacggacgg ttttaacagg gataaatgcc agtacgcagg cgaagagagt cagaaacgca
    16141 agggttaatg tcctaggcaa ttggggcaaa atcagcaacg gaaaaattcc gcaaattatg
    16201 catacgccga ctgtcgttat tttcatgcac ccctccctgt ttatggaaag tgtgcaactt
    16261 tgttgagcgg ttgtctggtg ggaattttgc gttccccgag gcacattcag aagaatttaa
    16321 cgaaagggtg aaaactgctc aaaactctgc gaggcagctt ccagttcctg attagagaat
    16381 tgaaaaaagc acccgacagg tgcttttctc gcgttcaagt ttgagtaaaa acttaaccgt
    16441 aaatattggc gcgatcgcgc agttctttac caggtttaaa gtgaggaacg tattttcctt
    16501 ccagttctac tttatcgcca gtcttcggat tacgtccggt acgtggtgcg cggtagtgca
    16561 aagagaaact gccgaaaccg cggatttcaa tacgctcgcc ctgcgcaaga gtcgaggcca
    16621 tatgctccag catctctttt actgcatctt caaccgtctt ggcgggaatg tgcgattgct
    16681 gggtggcaag tctttctatc aattctgact tggtcatgat tcctccggtt ccttaaaggc
    16741 aaattaagcg gctgctgttg ctgcattgat taagggcggc cgtagccgcc cttagtgctt
    16801 gattacagga cgaaacctgc aatctgtcaa gtaaactcaa caaacttcgg aataaaaatc
    16861 ccgaagagtc agagaattac tcgcctttag ctgctttgaa agcttcagcc attgcgttgt
    16921 tggagaagtt tgcatcttcc tgtttgttaa cagttgcgat tgcatctttc tcgtcagctt
    16981 cgtctttcgc acgaacagac aggctgattg cgcggttttt acgatcaacg ccggtgaatt
    17041 tagcttcaac ttcgtcgcca acgctcagaa ccagggtagc gtcttcaacg cggtcacggg
    17101 atgcttcaga agcacgcagg taaccttcaa cgccgtcagc cagttctacg gttgcgcctt
    17161 tagcgtcaac tgcagttact ttaccggtta cgatagcgcc tttcttgttc agagcaaccc
    17221 agttgttgaa cggatcttct gcgagctgtt taacgcccag ggagatacgt tcacgttctg
    17281 cgtcaacctg cagaacaact gcagcgattt cgtcgccttt tttgtattca cgaactgctt
    17341 cttcgcctgc aacgttccag gagatgtcag acaggtgaac caggccgtcg atgccgccgt
    17401 ccaggccgat gaagataccg aagtcagtga tagacttgat tttaccttca acacggtcgc
    17461 ccttgttgtg ggtttccgcg aactgctgcc acgggttagc tttgcactgt ttcagaccca
    17521 gggagatacg acgacgttct tcgtcgatat ccagaaccat aacttccact acatcgccaa
    17581 cgttaacaac tttggacggg tggatgtttt tgttggtcca atccatttcg gaaacgtgta
    17641 ccagaccttc aacgccttct tcgatttcaa cgaagcagcc gtagtcagtc aggttggtca
    17701 cgcgaccagt cagtttggta ccttccggat aacgtttagc gatagctacc cacggatctt
    17761 cgcccagctg tttcaggccc agggatacac gggtacgttc gcggtcgaac ttcagcactt
    17821 taacagtgat ttcgtcgccc acgttgacga tttcgctcgg atgcttaacg cgtttccagg
    17881 ccatgtcagt gatgtgcagc aggccgtcaa cgccgcccag atcaacgaat gcaccgtagt
    17941 cagtgaggtt cttaacgata cctttaactt ccatgccttc ctgcaggttt tccagcagct
    18001 gatcgcgctc tgcgctgttt tcggattcga taacggcacg acgagaaaca acaacgttgt
    18061 tgcgcttctg atccagcttg attactttaa attcaagctc tttgccttcc aggtgcagag
    18121 tgtcacgcac cggacgaacg tctaccagag aacctggcag gaacgcacga ataccgttca
    18181 gctcaacagt gaagccgccc ttaactttgc cgttgataac accggtaaca gtttcagcat
    18241 cttcgtaagc tttttccagc gtgatccagg cttcgtgacg tttagctttc tcacgggaca
    18301 gcagagtttc accgaagccg tcttctactg cgtccagagc aacgtcaact tcgtcaccta
    18361 cctggatttc cagctcgccc tgggcgtttt tgaactgctc agccgggatg gcggactcag
    18421 atttcagacc agcgtcaacc agtactacgt ctttgtcgat agcaacaaca acgccacgaa
    18481 cgatagaacc cgggcgggtt tcgatttctt ttaaggactc ttcaaagagt tgagcaaaag
    18541 attcagtcat gtttaatctt caggtttata tttaacgtcc acctggctcc atgccggatg
    18601 gggttgttta acatacccgc tgtcattcca ttgcaacggg ggtactgcaa attcggtcgc
    18661 ttatgcgaga gccaatttct ggcgcgcgta ttgtagcgct ttttcaatca attgctcaat
    18721 gcttaaggtg gtggaatcca acactaaagc atcggctgcc ggaaccagtg gcgctaccgc
    18781 tcggttacga tcgcggtcgt cgcgttcttt gatctcggcc aaaaggcgct cgaagttaac
    18841 actaaagccc ttctcctgca actgtagcat gcggcgatgc gcacgttctt ccgaggaggc
    18901 gtcaaggaaa attttcactg gtgcatcagg gaataccacc gttcccatgt cgcggccatc
    18961 ggcaatcaga cctggtaatt cgcgaaacgc gcgttggcga cgcaataatg cttcacgaac
    19021 gcgtgggaat gccgcgactt gtgaagccgc attcgccact tcctgagtac gaatttcgcc
    19081 gctgacatct tccccttcga ggatcacttc cagattgcca ttggtcgaca caaaacgtac
    19141 atccagatgg gatgccagcg gtaccagcgc atcttccgac gcaacatcaa catggtgatg
    19201 taatgccgcc aatgccagta cgcgataaat tgcacccgag tccagcagat gccattgcaa
    19261 cgcttccgcc atagccttac acaaggtgcc tttccctgca ccgcttgggc catcaatggt
    19321 aataaccggg gcaattgccg tcatctttat ctccttaaaa caggcgtacc gttaacataa
    19381 ccgcgcgcat tatacgcgcc gacgccacaa atcgttaacg ttgtgtgcaa aaaccagttt
    19441 tgtctgtatt cagatgggga tttattgcgc aattatagcg ggctggcaga agaccagccc
    19501 gaaagatgac aatgatttac ttaccagagg cgatacgatc acggatgtgt tgcgcacgct
    19561 ctgttgatgg tgggtgagag tcaaacatgg actgggtgcg accgccatcc aggctagcca
    19621 gtttttcaaa gctgccaacc agcccctggg tgctgatgcc acgtttcttc aacagatcaa
    19681 aagagaaatc atctgcttcg gactctttat cacgggagta tttagcatta atagcgcctt
    19741 ctgcgatatc acccaattgt gagcgggaaa gctgggaagc cacaccgctg gtagctgaaa
    19801 tggcatcgcg tgcggcaacg atcgcataag aagctttcat ttcagccagc gagtgaccca
    19861 acgcgacgtg gcccagttca tggcccagaa caccttcaat ttcgttgtca ttcatcatgt
    19921 ccatcaggcc actgtagaca cgaacacagc cgttcgccat cgcccatgcg ttgacgtcgc
    19981 tggtcatata aaccttatag ttgaccggcg tgccattaat attgttaccc aatgctttgg
    20041 cgatttttgc cagacgttta ccgtacttgc tggatttact tgcgacttga ttgccgctgt
    20101 ccatttcggc acagccctga ttggcaatcg cttttgcatc ggcatcgctt aatgttgctg
    20161 ctttataggc atttaaaccg gagctgatag ccatattggt atcaataccg tgggtatttt
    20221 gacaccctgt cagtaatgct gcagaggtcg caatcgccag cagtaattta gtattcttca
    20281 tgtgaatcat ccgttgatta atcatatata aatattatgt atcaacaact tacattgaca
    20341 agcgacgcta ccattaatca aaatgactca caaggtccga aaaaaaacgc cgacaat
//
LOCUS       DAJTRH010000069        20319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTRH010000069 DAJTRH010000000
VERSION     DAJTRH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..449)
                     /locus_tag="OO339_002709"
     CDS             complement(<1..449)
                     /locus_tag="OO339_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5927287.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVS"
     gene            complement(504..1355)
                     /gene="manZ"
                     /locus_tag="OO339_002710"
     CDS             complement(504..1355)
                     /gene="manZ"
                     /locus_tag="OO339_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5927288.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1368..2168)
                     /gene="manY"
                     /locus_tag="OO339_002711"
     CDS             complement(1368..2168)
                     /gene="manY"
                     /locus_tag="OO339_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5927289.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2231..3202)
                     /gene="manX"
                     /locus_tag="OO339_002712"
     CDS             complement(2231..3202)
                     /gene="manX"
                     /locus_tag="OO339_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5927290.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3665..5215
                     /gene="yoaE"
                     /locus_tag="OO339_002713"
     CDS             3665..5215
                     /gene="yoaE"
                     /locus_tag="OO339_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5927291.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5219..6817)
                     /locus_tag="OO339_002714"
     CDS             complement(5219..6817)
                     /locus_tag="OO339_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5927292.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6948..8312)
                     /gene="sdaA"
                     /locus_tag="OO339_002715"
     CDS             complement(6948..8312)
                     /gene="sdaA"
                     /locus_tag="OO339_002715"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5927293.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8496..9074)
                     /locus_tag="OO339_002716"
     CDS             complement(8496..9074)
                     /locus_tag="OO339_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5927294.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9078..10439)
                     /gene="pabB"
                     /locus_tag="OO339_002717"
     CDS             complement(9078..10439)
                     /gene="pabB"
                     /locus_tag="OO339_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5927295.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10513..10692
                     /locus_tag="OO339_002718"
     CDS             10513..10692
                     /locus_tag="OO339_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5927296.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10812..11171)
                     /locus_tag="OO339_002719"
     CDS             complement(10812..11171)
                     /locus_tag="OO339_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5927297.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11533..11877)
                     /locus_tag="OO339_002720"
     CDS             complement(11533..11877)
                     /locus_tag="OO339_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5927298.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12009..13919
                     /locus_tag="OO339_002721"
     CDS             12009..13919
                     /locus_tag="OO339_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5927299.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13977..14672
                     /gene="tsaB"
                     /locus_tag="OO339_002722"
     CDS             13977..14672
                     /gene="tsaB"
                     /locus_tag="OO339_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5927300.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14712..15293
                     /gene="yeaY"
                     /locus_tag="OO339_002723"
     CDS             14712..15293
                     /gene="yeaY"
                     /locus_tag="OO339_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5927301.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15499..17184
                     /gene="fadD"
                     /locus_tag="OO339_002724"
     CDS             15499..17184
                     /gene="fadD"
                     /locus_tag="OO339_002724"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5927302.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17266..18381
                     /gene="rnd"
                     /locus_tag="OO339_002725"
     CDS             17266..18381
                     /gene="rnd"
                     /locus_tag="OO339_002725"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5927303.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18435..19400)
                     /gene="yeaX"
                     /locus_tag="OO339_002726"
     CDS             complement(18435..19400)
                     /gene="yeaX"
                     /locus_tag="OO339_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5927304.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19456..20073)
                     /gene="yeaW"
                     /locus_tag="OO339_002727"
                     /pseudo
     CDS             complement(19456..20073)
                     /gene="yeaW"
                     /locus_tag="OO339_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         5011 a         5080 c         5270 g         4958 t
ORIGIN      
        1 gatacgagaa gcttataaat tttttccaga tcgtcgatat ttcgaacgcg gattaacagc
       61 cgacgctgtt ctaattgcat caccaacacg ccatcttccg acaagttcat cgctttgatt
      121 cggctatatt caatccagac attggcgaag aaaaaacctt tttgtttaaa gatgatcttc
      181 ggaatgcgga tccagaatat ataaaaaccc atcagagcca gtgcacttaa taaccatgtt
      241 gttattagcg cgccatgatt cgtgacgttg ttatagataa gaatgacaat caatccgacg
      301 aagataacgc tatcgatgcg accacgccgg agcaaaggaa ttgccagcag ggtggggccg
      361 ttacggcggg gcatgatgaa ctgatcgtag atcgcgaagg ccaggagtgc ggcgatgaaa
      421 agaatcagta ccaggtccgt gattgtcatt aatcctccag ataaaaaaac ggggccaaaa
      481 ggccccggta gtgtacgaca gtcttacagt cccagcaggc cgcaagcgta accagcgata
      541 ccgatgacga agaagccaac gatgatccac agcgggttaa cttttttgcg cagcagccac
      601 atacaagcaa atgtcagcag cagtggtaca aggcctggca ttaactggtc aagaatagtt
      661 tgcacggtgg tgacacgggt ctgtccatcc tgcccggtga tggtcgaaac caccagcggg
      721 atgttgacat gtgtccactt gttaaccaat gcccccatga caaacaggcc gaggatagac
      781 gccccttccg tcagtttttg caggaagcca ccacccatat ctttaacgat atcgatacct
      841 ttggagtaac catacgctac gccgtagtaa cgggttgcca gacgcaccag gttaaacagg
      901 atgaagaaca gcagcggacc taacaggctg ccgctcatcg cgataccggc acccagtgct
      961 gcaaataccg gacgtacggt tccccagaag atcgggtcgc ctacaccagc cagcggcccc
     1021 atcaaaccga ctttgatacc gttgatagca ccgtcatcga tctctgcgcc attagcacgc
     1081 tgttcttcca gcgccagggt tacgccgaga atcggtgcag ccacgaacgg ctgggtgtta
     1141 aagaactcca ggtgacggcg aatagcttgt ttacgagctt cgttgttctc agggtagagg
     1201 cgacgaattg ccggtaccat agagaagcag aaacccagtg cctgcatacg ttcgaagttc
     1261 catgaaccct ggaagaggtt agaacgcagg aatacgccac gaatatcact ttgagtgagt
     1321 tttttctcgg tggtagtttg agttgtatca accatttcgc tcacctgtta gtccagttcg
     1381 ttatcgagat cgttgttacc agctgcctga gcaggcgcac cggctacgcg gttgtatttc
     1441 gggctaagtt ggatgtagag cactgccata acagtaccaa tcacacccag agcaaccagg
     1501 ttaaagttgg tgaatgctgc ggttacgaag ccgaggtaga agaacggcat caggtagcca
     1561 gcacgcatca tgttgataac catcgcataa ccaaccacca cgatcatgcc cccggcgata
     1621 ttcagaccat tggtcaccac ttccggaatc gcattcagca tgttctgtac ttcgctggta
     1681 ccaacagaca gcgcaacgat gacggccgga atagccacac gcattgcttg caggaacaga
     1741 gaagaaacgt ggatccagga aatcgctgtc aggttgccgt tatcagcagc cttatccgca
     1801 gcgtgctgga aagcaacggt aatagtacga acgatgatgg tcagtacctg gcccgcagcg
     1861 gccagaggga ttgccagtgc gatacctgca ccaatgctct gatgacctgc gataaccaga
     1921 atggtagaaa tgatagaagc cagagcggcg tcaggcgcaa ctgcagcacc gatgttcatc
     1981 cagcccagcg cgatcatttc cagcgtacca ccgataataa taccggtttt catatcccca
     2041 agaacgatac ccaccagggt acacgcgatt agcggacggt gaaactgaaa ttcatcgagg
     2101 attgatccca tacctgcgat acaggctacg ataaatacca gcacaatttg aagagtggta
     2161 atctccattg tacttctcct gtttacgtaa gacttaagtg tgaaaactga atgataatca
     2221 acacaatacg ttacttatcg attttgctga tcagatccat cattttcagt ttcggatcgg
     2281 tggaaacctt acggacttcc agctcaatac cgcgtgcatt cagttttttg aacgcctcga
     2341 tatctttttc atcaaccgaa accgcgttat tcacctgggt tttaccctga cggaatgcca
     2401 taccaccgac gttaacagag gtgattttca cgccgccttc aacgagacgc tctacatctg
     2461 ttgggttggt aaataacagc attacgcgtt cgccagcata tttcgggttg ttgtagacgc
     2521 gaatcatttt ggcaacatca actacgtgtg ctgttacgcc cggaggtgca acctgggtga
     2581 gcagtgtctt acgaacggta tccgcagcca cttcatcact aacaacaata atacgggaga
     2641 cattggtttc tttggtccag cgggtggcga cctgaccgtg aatcagacgg tcgtcgatac
     2701 gcgcaaggcc aataaccatg tagtcgtttg gccccattgg ttttgccgga gttggagccg
     2761 cttttggtgc tgcggcagcg ggtgctggcg cggctttttc aaccggtttg gctttcagtg
     2821 ctttcacgcc ttcacggcct gtttctactg ccagcgcaac cagttcatca aagctcgggt
     2881 catcatcacg ggccattaac gtttccacga gcattggaat gttaacgcct gcaatgactt
     2941 cataatgctc tttgtcgacg acaatgcggc tggcagcatt gaacgggctg cctccccatg
     3001 tatcaacgag aaacagcacg cctttagtgg tgtcgagttt tgccaactga gcgttgtatt
     3061 tttcaatcag cgtttcggca ttttcacctg gaacgaaatc gatccagccg acgttttcct
     3121 gctcgcctaa cagcatttct gccgttttaa gcaactgctc tgcagcccaa ccatgtgtgc
     3181 ctataacaat agcaatggtc acttgctacc tcctttatta tcgttaacac ctcaacgtgc
     3241 cagatgtatt tttgaatcgt cgtccacaat cgaatcgatt cagataaggg ctacaaccaa
     3301 aaacccctgc gtttcgcgat ttattttaga tatcgaaaaa attattttat gtgatgaaga
     3361 tccgtaattt aactttcgat tatcagaaat ttcgaaaggt aaaatatcct tgtcacattc
     3421 gtttgcaaag gaaggtaaat ctttgccaaa tcagaggcgt ctctgatatg tttaactccc
     3481 gtttaacaac catggagtat agggcagtag cccgcagtat ggatcgtcac cgacgtcatt
     3541 tcagcatcag gccttttaac gcctgccttt ctggcactct atgccgcacc tttcgtttgc
     3601 attttgtcgt tacgcctgca ttatttctgg cgtcgaatag ctattcctta agcaggagct
     3661 tgtcatggaa ttcttaatgg acccctcaat ttgggcgggg ctactcacgc ttgttgttct
     3721 cgaaattgtg ctgggtatcg ataacctggt cttcatcgcc attcttgctg acaaactgcc
     3781 gccaaaacaa cgcgataaag cgcgtttgct ggggttatca ctggcgctga ttatgcgtct
     3841 ggggctgctg tcgctgattt catggatggt cacgctgacc aaaccgctat ttaccgtcat
     3901 ggatttctcc ttctccggac gcgatctgat tatgttgttc ggggggatat tcttgctgtt
     3961 caaagcaaca accgaactgc atgaacggct ggaaaaccgc gatcatgatt ccggccacgg
     4021 taaaggctac gccagtttct gggtggtcgt cacacagatt gtcatccttg acgccgtctt
     4081 ctcgttggat gcggtaatta ctgcagtagg gatggttaac catctgccgg tgatgatggc
     4141 ggcggtagtg attgcgatgg cggttatgtt gctggcatcg aaaccgctga cgcgattcgt
     4201 taaccagcac cccacggtgg tggtgctctg tctgagcttc ctgttaatga ttggtctgag
     4261 tctggtggca gaaggtttcg gtttccacat tccgaaaggt tacctgtatg ccgcgattgg
     4321 cttctcgatc attatcgaag tgtttaacca gattgcgcgt cgcaacttta ttcgtcacca
     4381 gtcgactttg ccgctgcgag cgcgtactgc cgatgccatc ctgcgtttga tgggcgggaa
     4441 acgtcaggcc aatgttcagc acgatgccga taacccgatg ccgatcccgg aaggtgcatt
     4501 tgccgaagaa gaacggtaca tgattaacgg cgtactgacg ctggcgtcgc gttctctgcg
     4561 cgggatcatg acgccgcgcg gtgaaataag ctgggttgac gctaatctcg gggtcgatga
     4621 aatccgcgag caactgctct cttcaccgca cagtctgttc ccggtatgtc gcggtgaact
     4681 ggatgaaatc atcggtatcg tacgtgctaa agaactgctg gtggcgctgg aagagggcgt
     4741 tgatgtggcg gcgattgctt cggcgtctcc ggcgattatc gtcccggaaa ccctcgatcc
     4801 gatcaacctg ctgggcgtgc tgcgtcgcgc tcgcgggagc tttgttatcg tgaccaacga
     4861 gtttggtgtg gtacaaggct tggtcacgcc gctggatgtg ctggaagcca ttgcgggtga
     4921 attcccggac gctgacgaaa cgccggaaat cattaccgat ggtaacggct ggctggtaaa
     4981 aggcggtaca gatttgcatg ccttgcagca ggcgcttgat gttgagcacc ttgccgatga
     5041 cgatgatatc gcgacggtcg cgggcctcgt gatctcggca aatggtcaca ttccccgtgt
     5101 gggcgatgtg attgatgtag ggccactgca tatcaccatc tttgaagcca atgattatcg
     5161 tgttgatctg gttcgcattg ttaaagagca accggcgcac gatgaagatg agtaagcgtt
     5221 aacgtaacga cataatgggc gtgatatgtc cattatgccg ggcgggcggc ggttggctgc
     5281 ccgccagcca tttgggaaaa tcacgtagcg gcatcggctg tgcgtacaaa tacccttgca
     5341 aaatatgcac gccatgacgg cgcaaatact tcgcctgttc ttgcgtttcc acgccctccg
     5401 ccaccagttc aatattcagt ctttgcccca gcgcgatgat gatatcggtc accgtcgagt
     5461 taaccgcgtc agaacctata gctgcggtaa atgacttatc aattttcagc acgtcaggac
     5521 gtaatgtttc aagccaggaa aacgagctgt tgccggtgcc gaagtcatca atcgccagtt
     5581 tgacgttttt acgatgcagc tcgcgggcaa tccgataatc aacatccagt aaggcatcgc
     5641 gttcggtgat ttcgaggatc agttgctgaa tcgggtgagc gctaaaccag tactgattga
     5701 gatcttttat cagcacacca cggcgaaaat ggctgggtgc gacgttaatg ccaacatgaa
     5761 actgactact catcgggaaa acatggtgct gacgaatggt ttctgccatc acatagtggg
     5821 tcagtggcac aattaaatga tgttcttccg cgataggaat aaacacatcc ggtgaaatcc
     5881 agccctgacg cggattattc cagcgcagca aaatctctac accaatacat tgctggctgc
     5941 gcgcattaag caaaggctga cagaacaatt cgaactcatg ttgcgccagg cccagattaa
     6001 tttcgcggga aaagctcatc cggtaagcgg tcgccagcca ggcgatatag cccaccagta
     6061 aacttagcag caccgccagc ggtaattgtg ttggaagata gtgccatgcc agcgccgtag
     6121 cccccggacc atttacgtta atggtaaaag gaaagctttg cgaagaaacc tgatagtttt
     6181 cattgtcttc aggttgcgga agggaatcta ccagcccatt accatagagc aaatgccgtt
     6241 tgtccaccgt caggctggcg ctactgattt gcggcagttg tggctcaagg agcattgccg
     6301 tcagtaagtc gatgttaatc atctctatga ccccagcatt gctactgccc gctgcaggcg
     6361 tccattgaat caaaaccgga ctgcctttaa tcagggcacg gtcgatcgtc aggcgtaaaa
     6421 gtggttgcgg tgcaggaagt tcagccagaa tgtccacgac gggcacattg cgataaccaa
     6481 aaatgctgga gcaataaagt gtgccgtctt gcaccaggcc aatggatcgc acagtttgga
     6541 gttttgccgc ctgtttacgt aatggcaaat gggcgacaga gcagggcaga ccaatcagcg
     6601 gaagcaagac tttgctacct gcctgtaggg gaagcagtac tttatctaat tcctctacag
     6661 cgtgattggc gaattgtact acccgttgtt gatttaagtt acgctgtgaa ataaatcgca
     6721 cgctcagggt cgaagcgagc gtaacgagtg cgcaaatcgt acaaacaatc attcgattac
     6781 ggcgataggt tttaatgatc cgttgtgctt tttgcatgcg ggccaccctg taagcgtatg
     6841 gcgacaaacg cctgacgcaa cagtggaagt gtagtgggga atattacagc agacgagaaa
     6901 gcgggtataa attcgcccat ccgttgcaga tgggcgagta agaagtatta gtcacactgg
     6961 actttgattg ccagaccacc gcgtgaggtt tcgcggtatt tggcgttcat gtccttaccg
     7021 gtttcgtaca tcgtttcgat gaccttatcc agcgagacgc gcggtgcact ggtgcggcgc
     7081 agagccatcc gtgcggcgtt aatcgccttc acagaggcaa tggcattacg ctcaatgcac
     7141 ggcacctgaa cctgccctgc aaccgggtcg caggttaaac caaggttgtg ttccatgcca
     7201 atttccgccg ccacgcaaac ctgttccggg ctaccgccca gcagttctgc aagacccgca
     7261 gcagccattg aacaggcaac acccacttcg ccctggcaac caacttccgc accggaaata
     7321 gaggcgttca ttttatacaa tgcaccaatc gcgcccgctg ccataaagta acgggtatag
     7381 atgtctgggc tgaccgattc aataaagtgg tcatagtaag ccagcactgc cggaacgata
     7441 ccgcaggcac cgttggttgg cgcagttacc acacgaccac cggcggcgtt ttcttcgtta
     7501 actgccagcg caaacatgtt tacccagtca atgacattca tcggatcgtt agacagttta
     7561 tcgctggaaa ctaacatccg gcgcagggca gacgcacgac gtggcacgcg cagcgggcct
     7621 ggcagtacac cttcggtgtt catcccgcga tcgatacatg cctgcatggt ttgccagaca
     7681 tgcgcgaaat actcgtcgat ctctttcttg ctgtgcagcg ccagttcgtt ctgcatagcg
     7741 agaccagaca gcgaatagcc ggtttcatta cagtacgcga gcagttcggt ggcagatttg
     7801 aacggatacg gcacgctcac ttcgttggca gcatcctgac caaagtgttc ttcatcgacg
     7861 ataaaaccgc cgccgatgga ataataagtt ttgctgtaga cgacttcatc gccgttatag
     7921 gcgtggattt gcataccgtt ttcatgcagc ggcaggttgc cgttatgaaa acgcatcccg
     7981 ttgtcgcgcg ggaaatccac ttcatgccgt ccctgtgcca gcagcagacg ttcgcgctct
     8041 tttacgtcgc gaataaaacc gggaatactg tcgatatcca cggtggcagg ttcgttacct
     8101 gcaagaccca taataatggc gatatcggtg tggtggcctt tacccgtcag cgacagtgaa
     8161 ccataaacgt ccacggcaac gcgagtaacg ctatccagta agcctttttc gaccagatca
     8221 tcgacgaact gtttacccgc cttcataggc cctacggtat gggaagatga gggaccaatc
     8281 cccaccttaa acatgtcgaa tagactaatc acgataatac tcctgacaag gtgactggac
     8341 ttccagtaac gaactaataa cagcgcatag tgtaagaggg aacgcggcga ctggcttaac
     8401 tattcacatg aattaaacta atcaaaaacc gcgatttaac taatattgcg atgattgtct
     8461 caagtagtgt tttgtagatg taaagataag tatagtcagg gggtcacacc aatttgcagc
     8521 gccagctcac gaattatgcc tgcggtcatt ccccatacaa aatactgttc gtaccaggac
     8581 agccataccc gatgtgaatc gccacggcgg tagatatcta aagggtgata acgacccaga
     8641 tgtaatgcct gggcgagcgg catttcaaac accgccgaga cttcatcttc actggcgcga
     8701 tacggcagat cgggcgggat aatgccgacc actggggtta cttgatagcc agttacgcta
     8761 tcgacgggcg gcagcacgcc gataacttca acggcggaag gcggtatagc gacctcttct
     8821 tcagcttcgc gcagcgcggc ggcgataact gatgcgtccg tgtcatcgac tgcacctcca
     8881 gggaatgcca cttgtccagc gtgtttacgc agatgaattg aacgctgagt cagcaacaac
     8941 cccggttgcg gtcgacggac gatggggatt aacacggcag cctgacgatg atttaggggt
     9001 tcccggttaa tttgcgggcg caaaagttga aagcgagata aaaaatcatc aagcgtcagg
     9061 ctacggtatt ccacgtctta cttctccagt tggcgtaata tcttattaac tttatcaaaa
     9121 gtttcctgat attccgcttc ttcctggcta tcggcgacaa ttccaccgcc cgcagagcag
     9181 tatatttgtc cgttaatggc agtcagcgtg cggatagtaa tgctggtatc catgttgccg
     9241 caaaagctca aatagccaat gctgccgcac caggcattac gtcgctgcgg ttccagttcg
     9301 tcgataattt ccatagcccg tactttcgga gccccggtta ttgagccacc aggaaaagct
     9361 gcgcgcagca gatcgctggc gtgtaactgt tctggtagtc gcgccgttat agtgctgacc
     9421 agatgatgca cggcagggaa gggttccacc acgaagagct ctggtacttt taccgaaccg
     9481 gctacggcaa cacgaccgat atcattacgc attaaatcga caatcatcag attttcggca
     9541 cgatctttcg ctgagttcgc cagtttttct gcttgtttgc tatcttcctg aggatcgggc
     9601 aggcgtggta gcgtgccttt aatcgggcga gtctggattt cactattatc acaaagaata
     9661 aaccgctctg gcgaaaggct taaaattgca ccctgttcaa gacgtaaaaa agcgctaaat
     9721 ggcgcgcggt tggcctgatt aagctgaagg aatgcctgcc attcatcgcc agaataggtc
     9781 gcatgaaaac gctgggcgag attcacctga tagcaatcac cgctgtgcag atattcctgt
     9841 acctggcgaa atttttcgcc gtactgctcg tgggtcatat tggattgcca gtcggaagtg
     9901 agcgtgaaat cttcctgcgg cgagaattgc tggctttcca gccaggcccg acgggcattg
     9961 acatcattat gactcagcaa agaaactgta tgacgctggt ggtcgacaat gagcgcccaa
    10021 tcgtagatac ccactgccat atccggcaga acgatatctt gttccgcaat ttctggcagt
    10081 gactcaaaac ggcggcccag atcgtagcca aacaacccca gtgcgccgcc ctgaaatggc
    10141 aaatcttcgt tatgcgttgg gcgaatgtct gcgcgatcca gcacctgctg gagcacctgt
    10201 agcgggtcat cagtggtcgt tgtgcgtttt tcgctttcac taacaacggt ttctttacct
    10261 aaagtggtta aagtgcaaat cggatcggcg accacaatat caaagcggct atacggatga
    10321 tcggcatagc cggagtgtaa aagcatcgcc cacggcaggt ggcttaagcg ggagaaataa
    10381 aattcagcgg cgtcctgacg ccagggtaaa gtaatcacag tgggagataa cgtcttcatc
    10441 agtcctgact ctactggcta tgtgccgggt agcatgaaat aattagcgcc aacaatttag
    10501 caggagttaa caatgtttgc aggtttacct tcactcatcc atgaacagca gcaaaaagct
    10561 gtcgagcgga tccaggaact gatggcacaa gggatgagta gcggccaggc aatcgcgctg
    10621 gtggcagaag agctgcgtgc caaccactcc ggcgagctaa tcgtggcgcg ttttgaggat
    10681 gaagacgagt aacacactaa tccgtatcga tttcgttata gaagccgatg atcatttgat
    10741 tgtgagaaaa aggacgcttt ttatatgtga ttaaaagcgt cctgacccat ttatgctgat
    10801 ataaatttaa ctcagaccag tgcttttagt tcttcctgca tatatgttga aaagtattca
    10861 ggatttttaa tcacagagcg ttcacctgtc tccatttttt ttgcccatcc aaccattttt
    10921 tccgtgaacc cagggttcca gccttcctgg tcagctcgcg ccgtaatatc agcggcactc
    10981 gcgggtactc ccagttcttt taaatattta aagattccct tcgccgtgct ttcattcatc
    11041 gaacttggtg ttggcgctga tacatccatg gcaccaatga aatccagggc tttatctatt
    11101 acagcaggca tattcttatc cttaaaatta accatgttat cgataaggct atatatgacc
    11161 ttaatttgca tctaatatca aagggattat aacgatatgg cacgagttaa cctgttgaca
    11221 ttcaggtgtt ttaaaggtag cagctcatgg tgtgttatcc agtaagatgg agaaaattaa
    11281 cggtcagccc gcacgctata accagattgt tcagctaaga aagaaaacca gagttgctaa
    11341 tcaaagggcg aacacaacgg catggttatg tttatttata tagttagttt tatagataaa
    11401 attgaaatta taaatataaa taatgaatta gcagaagcat caacagtaat atcttattct
    11461 ctggtcgatt tatgtaaaac cgaccagtat tatcgataaa ttatcgtctg acttattttg
    11521 cttcgataaa gcttacaccg cagccacaat cttaatttca actttatact tcgggttcat
    11581 caaacccgct tgtaccgtgc agcgcaccgg cgcatgaccc gcgacaaccc aggcatccca
    11641 cgctttattc atcgccgcga agtcgttttt atcggccagg aaaatggtgg catccagaat
    11701 gctcgattta ttgctgccct gtttttccag cacggcgtca atctgtgcca gcgtgttggc
    11761 ggtttgctca aaggcatcgg cgtcgaggtt ttccggtaca ccagtgtagt agagcgtgtt
    11821 gttgtggatt actacatcag accagcgggc ttcagcatcg atacgaacga tagtcatcat
    11881 tttgtcctca ttatacttcc attttcgtgc tgcaaagact gccacaatgt gaggttgccg
    11941 tcaccacttc aactggcgaa agcgccctca tcctgacata atccctgttc aaatcaacag
    12001 ggggtagtgt gacggacgat tttgcaccag acggtcagct ggcgaaagcg ataccaggct
    12061 ttaagccgcg agaaccacag cgacagatgg cggtagccgt cacccaggcg atagaaaaag
    12121 gccagccgct ggtggtggaa gcaggaaccg gtacgggcaa aacctacgct tacctggctc
    12181 ctgcgctgcg ggcgaaaaag aaagtcatta tctcgaccgg ctcaaaagcg ttgcaggatc
    12241 agctctacag ccgcgatttg ccaacagtct caaaggcatt gaaatatacg ggcaacgtgg
    12301 cgctgctgaa agggcgctca aactacctct gcctcgaacg tctcgaacag caggcgctgg
    12361 cggggggcga tctgccggta caaatcttaa gcgatgtgat cctgctgcgc tcctggtcta
    12421 atcaaacagt cgatggtgat atcagcacct gcgtcagcgt ggcggaagat tcacaggcgt
    12481 ggccgctggt caccagcacc aacgacaact gtcttggcag cgactgcccg atgtataaag
    12541 attgctttgt ggtcaaagca cgtaaaaaag cgatggacgc cgatgtggtg gtggtaaacc
    12601 atcatctctt tctggcggat atggtggtta aagagagtgg atttggcgag ttgatccctg
    12661 aagcggacgt catgatcttc gacgaagccc accagctacc ggacattgcc agccagtatt
    12721 ttggtcagtc actctccagt cgacaactgc tcgacctggc aaaagacatc accatcgcct
    12781 accgcaccga attaaaagac acccagcagt tacaaaagtg cgctgatcgt cttgcccaga
    12841 gtgcgcagga ttttcgtctg caactcggtg agccaggtta tcgcggtaac ctgcgtgagc
    12901 tgttagctaa tccgcaaatt cagcgggcat ttttactgct cgatgacacc ttggaacttt
    12961 gttatgatgt ggcgaaactg tcactggggc gttccgcctt gctggatgcg gcatttgagc
    13021 gcgccacgtt gtatcgcaca cggctgaagc ggctaaaaga gatcaatcag ccgggctaca
    13081 gctactggta cgaatgcact tcgcgccatt ttactctggc tctcacgccg ctcagcgtgg
    13141 cggataaatt caaagagtta atggcgcaaa agcccggtag ctggatcttc acctcagcaa
    13201 cgctgtcggt gaacgacgat ctgcatcatt tcacctcgcg gcttgggata gaacaggcgg
    13261 agtcgttgct attgccaagc ccgtttgatt acagccgcca ggcgttactc tgtgtgccgc
    13321 gcaatctgcc gcaaaccaac cagccaggtt ctgctcgcca gttagcggca atgctgcgac
    13381 cgatcatcga agctaacaac ggtcgttgtt ttatgctttg tacctcgcac gccatgatgc
    13441 gcgatctggc cgagcagttc cgcgctacca tgacgcttcc cgttttgttg cagggggaaa
    13501 ccagtaaagg ccaactgttg cagcagtttg tcagtgccgg taatgcgctt cttgtggcaa
    13561 ccagtagttt ctgggaaggg gtggacgtgc gtggcgatac attgtcattg gtaattatcg
    13621 acaaattgcc gtttacctcg ccggatgatc cactgttaaa agcgcgcatg gaagattgtc
    13681 gtttgcgcgg tggcgacccg ttcgatgaag tgcaactacc agatgccgtc attactctca
    13741 aacagggggt aggtcgactg attcgcgacg ccgacgatcg tggcgtgctg gtgatttgtg
    13801 actatcggct ggtgatgcgt ccttacggcg cgacgtttct cgccagtctg ccgcccgcgc
    13861 cacgcacccg tgacattgcc cgtgcggttc gtttccttgc gataccatcc tccaggtaat
    13921 ttgttacgga gtatggtatg atgcgcgcct ttttattgat ctaagcacga agacccatgc
    13981 gaattctggc tatcgatacc gcgacagagg cctgctctgt cgccctgtgg aacgacggta
    14041 ctgtcaacgc tcattttgag ctttgccctc gtgaacatac tcaacgaatc ttaccgatgg
    14101 tgcaggatat cctgaccacc agcggaactt ccctgactga tattaacgct ctggcttacg
    14161 ggcgcggccc cggtagcttt accggcgtgc gcattggtat tggcatcgcg caagggctgg
    14221 cgcttggcgc ggaattaccg atgattggcg tctccacgct aatgacgatg gcgcaagggg
    14281 cgtggcgcaa aaacggcgca acacgcgtgc tgtcagccat tgatgcgcga atgggcgaag
    14341 tttactgggc cgaatatcag cgtgatgaaa acggtatctg gcacggtgaa gaaaccgaag
    14401 ccgtactcaa acccgaactc gttcatgaac gaatgcaaca gctttccggc gaatgggtga
    14461 cggtagggac gggctggcaa gcctggccgg atctcggtaa agagagcggg ctggttttgc
    14521 gcgatggcga agtgttactc cccgctgcag aagatatgct gccgattgct tgtcagatgt
    14581 ttgccgaggg taaaacggtg gcggtggaac atgccgagcc ggtttatttg cgtaacaacg
    14641 tcgcatggaa gaaacttccg ggcaaagaat gaatcttaag agtatgccct gagaaaaaaa
    14701 ggagtcgcat catggcggtt caaaagaatg ttatcaaagg catactggca ggtacgtttg
    14761 cgctaatgct gagcggttgt gtcactgtgc cggacgccat taaaggcagc agtaccacgc
    14821 cgcaacaaga tttagttcgg gtgatgagtg cgccgcagct gtacgttggt caggaggcac
    14881 gctttggtgg caaagtggtt gcggtacaaa accagcaagg gaaaacccgc ctggaaattg
    14941 ctaccgtacc gctggacagc ggagctaggc cgacgctggg agaaccttct cgcggtcgca
    15001 tttatgccga tgtgaacggt tttctggacc cggtggattt ccgtggacaa ctggttacgg
    15061 tagtcgggcc aatcaccggt gcggttgacg gcaaaatcgg caatacgccc tataaattta
    15121 tggtgatgca agtaacgggt tacaaacgtt ggcatttaac ccagcaggtg attatgccgc
    15181 ctcagccgat tgatccatgg ttttatggcg gtcgtggctg gccctatggc tacggcggat
    15241 ggggctggta taatcccggc cccgcgagag tacaaacagt tgtaaccgaa taattgcttg
    15301 tttttaaaga atgcagaaac agcggctggt ccgctgtttc tgcattctta cggtaaagat
    15361 aaaaataaat agtgacgcgc ttcgcaacct tttcgttggg taattatcaa gctggtatga
    15421 tgagttaata ttatgttaac ggcgtgtata tcatttgggg ttgcgatgac gacgaacacg
    15481 cattttagag gtgaagaatt gaagaaggtt tggcttaacc gttatcccgc ggacgttccg
    15541 acggagatca accctgaccg ttatcaatct ctggtagata tgtttgagca gtcggtcgcg
    15601 cgctacgccg atcagcctgc gtttgtgaat atgggggagg tgatgacctt ccgcaagctg
    15661 gaagaacgca gtcgcgcgtt tgccgcttat ttgcaacaag ggttggggct gaagaaaggc
    15721 gatcgcgttg cgttgatgat gcctaactta ttgcaatatc cggtggcgct atttggcatt
    15781 ttgcgtgccg ggataatcgt cgtaaacgtt aacccgttgt ataccccgcg tgagcttgag
    15841 catcagctta acgatagcgg cgcatcggcg attgttatcg tgtctaactt tgcccacact
    15901 ctggaaaaag tggttgataa aaccgccgtt cagcacgtaa ttctgacccg tatgggcgat
    15961 cagctatcta cggcaaaagg cacggtagtc aatttcgttg ttaaatacat caagcgcttg
    16021 gtgccgaaat accatctgcc agatgccatt tcatttcgta gcgcactgca taacggctac
    16081 cggatgcagt acgtcaaacc cgaactggtg ccggaagatt tagccttcct gcaatacacc
    16141 ggcggcacca caggtgtggc gaaaggcgcg atgctgactc accgcaatat gctggcgaac
    16201 ctggaacagg tcaacgccac ctatgggccg ttgctgcatc caggtaaaga actggtagta
    16261 acggcactgc cgctgtatca catttttgcc ctgaccatta actgcctgct gtttatcgaa
    16321 ctgggtgggc agaacctgct tatcactaac ccgcgtgata ttccagggtt ggtaaaagag
    16381 ttagcgaaat atccgtttac cgctatcact ggcgttaaca ccttgttcaa tgcgttgctg
    16441 aacaataaag agttccagca gctggatttc tccagtctgc atctttccgc aggcggtggg
    16501 atgcctgttc agcaagtggt ggcagaacgc tgggtgaaac tgaccggaca gtatctgctg
    16561 gaaggttatg gcctgaccga gtgcgcgccg ctggtgagcg ttaacccgta tgatattgat
    16621 tatcatagcg gtagcatcgg tttgccggtg ccatcgacgg aagccaaact ggtggatgac
    16681 gacgacaacg aagtaccacc gggtcaaccg ggtgagcttt gtgtcaaagg accgcaggtg
    16741 atgctgggtt actggcagcg tccggatgct acagatgaga tcatcaaaaa tggctggtta
    16801 cacaccggcg acatcgcggt gatggatgaa gaaggattcc tgcgcattgt cgatcgtaaa
    16861 aaagacatga ttttggtttc cggttttaac gtttatccca acgagattga agatgtcgtc
    16921 atgcagcatc ctggcgtaca ggaagtcgcg gctgttggcg taccttccgg ctccagtggt
    16981 gaagcggtga aaatcttcgt agtgaaaaaa gatccatcgc ttaccgaaga gtcactggtg
    17041 actttttgcc gccgtcagct cacgggatac aaagtaccga agctggtgga gtttcgtgat
    17101 gagttaccga aatctaacgt cggaaaaatt ttgcgacgag aattacgtga cgaagcgcgc
    17161 ggcaaagtgg acaataaagc ctgagcgtta agtcagtcgt cagacgccgg ttaatccggc
    17221 gttttttttg acgcccacta aagagaaaac aatttgaatt accaaatgat taccacggac
    17281 gatgcgctgg cttctttgtg tgaagccgtc cgtgtctttc cggcgatagc cctggatact
    17341 gaatttgttc gtacgcgcac ttattacccg cagctggggt tgattcaact tttcgatggc
    17401 gagcatctgg cgctaattga tcctctcggg atcaccgact ggtcaccgct gaaagcgatc
    17461 ctgcgcgatc cgtccatcac aaaatttctc catgcaggca gtgaagatct ggaagtgttc
    17521 ctcaatgtct ttggcgaatt accacaaccc ttgattgaca cgcaaatcct tgctgccttc
    17581 tgcggacgcc cgatgtcatg gggtttcgct tccatggtgg aagagtattc cggcgttacg
    17641 ctggacaaga gtgaatcgcg caccgactgg ctggccagac cgctgaccga acgtcagtgt
    17701 gaatacgcag cggcggatgt ctggtatctg ttaccgatca ccgccaagct tatggtagaa
    17761 acggaggcct ccggctggct acctgcggcg ctggatgaat gccgcctgat gcaaatgcgt
    17821 cgtcaggaag tcgttgcgcc ggaagatgcc tggcgtgata tcaccaatgc ctggcagtta
    17881 cgcacacgcc aactggcctg tctgcaactg ttagccgact ggcgactgcg caaggcgcga
    17941 gagcgtgatc tggcggtgaa ctttgtcgtg cgtgaagagc atttgtggtc ggtcgcgcgt
    18001 tatatgccgg gaagtttagg cgaactggac agcctgggtt tatccggtag cgaaatccgc
    18061 tttcacggta aaacgctgct ggcgctggtg gaaaaagcgc agacattgcc ggaagatgcc
    18121 ttaccgcagc cgatgcttaa cctgatggac atgcctggtt atcgtaaagc gtttaaagcg
    18181 attaagtcgc tgattactga cgtgagcgaa acgcataaga tcagcgccga attgctggca
    18241 tcgcgtcggc aaatcaacca actgctgaac tggcactgga aactgaaacc gcagaacaat
    18301 ttgccggagc tgatttccgg ctggcgtggt gagctgatgg cggaagcatt acacaattta
    18361 ttgcaggaat atccgcagta aaatcttccg aagccggact gggcgcgctc agcgccacat
    18421 ccggctttgg caaactacaa atccaacacc aggcgtttac ccttcgcacg cgaacaacag
    18481 atcaacatac tttgctggct ggcacgctct tcatcgctaa aatactgatc ccgatggtca
    18541 gcttcacctt ccagtattgc cgtttcgcag gttccgcata ccccttcacg acataaacat
    18601 tccactttcg cggctttatt attttcaata acctgcaaaa tagtcatctc ttccggcacc
    18661 acaaactctt ttccggaacg ggcaagcacc agggtaaatg catcgccggt tttgtcttcg
    18721 atagcaaatt gctcaaagtg cagtgtatcg gcggcgatgt ccagacgcgc agcttcactt
    18781 cttaccgctt caattagcgc ctcggggcca caggtataaa cgtgtgtgcc aggttcgata
    18841 tccgccaata atcgcgctaa ttccagtcgt gttccggttg atgaatgatg caaatggact
    18901 ttctcagcct gcggatgctg gactagctca gcacgaaatg cgcaactttc tggatttcgc
    18961 gagcagtaat gtagctgcca gtcgatgtcg ctgtgttgca actctgccat gtgcgacagg
    19021 aaaggggtga taccaatacc gcccacgata aacagatgct ttctggctga gggaattagc
    19081 gcaaacaggt tattaggcgt tgagatcgtt aaccgatcgc ccacttttac ctgctgatgc
    19141 aaaaagcggg aaccgccgcg cgagttttct tccagtcgaa cggcaatctg ataactagag
    19201 gtgttatgcg gtgaactcag tagtgaatac gcattgctgt actggttatc accatcgctc
    19261 atctgcacta ggatgtgact tcctccggtg aacgcaggca gcggtttgcc atcggttgcc
    19321 accagcgtga agcgtttcac ctgttcggta aggggttcaa cctggctcac ctgtacttca
    19381 aacatttgat agtctgacat tgctgtactc catcaaaaat cagcccagcg gcggaaaata
    19441 ccgccgccga tgtcattagt ccttaaacac ctgcgccagc agattatgga aatgggcgat
    19501 accatgttcg gaaatgccac taccgctact gtcggccatg atgcgcccct gaccacgata
    19561 gccacgcgat ttcagccctt tctgtacgct ttcaaccaga cgtaaatctt ccggatggaa
    19621 cacatcgcga taccactcaa tcagcgattt ttgctcgtcg gttaactctt cattggtgaa
    19681 gtaaatatcg tagttttgca gggtagtttc agaatccacc gggaattcat aaatgaccgt
    19741 catcatccct ttgatcgggg tgacgttcag catcgtgcac ggccacagcc agaaaccgtg
    19801 gaatgccgca tccgtaccct cttcaaattt aaacgactgt tcggacggtt tggcgaaacc
    19861 gtattgcagc gtccagttac cgtgcatggt gtgccagtaa cgatcaacct gtacggagtc
    19921 ggagaaacct agatgcgccg gaccacagtg atagcactcg agatagttat cgacaatgtt
    19981 cttccagttg gcaggcgtgc gggtggtaaa gcgggccgct agtttcagat tgtggacttc
    20041 cgggcaggct tccagcactt tcgcgcccag accggtgtat tgattcactt gaagtacgaa
    20101 aaaaaaccgg gaggacattg gattattcgg gttctgatgg gattagattt ggtggggctt
    20161 gcaagcctgt agcgcaaatt ttagtcgtta atcaatgaaa cgcgaaagat agtaaaaaat
    20221 tgcttttgtt tcattgaaaa tacgaaaaac aaaaacactg caaatcattt caataacagc
    20281 ttcaaaaaac gttcaaaacc gataacaacc aggctgtaa
//
LOCUS       DAJTRH010000070        20182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTRH010000070 DAJTRH010000000
VERSION     DAJTRH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            83..352
                     /locus_tag="OO339_002728"
     CDS             83..352
                     /locus_tag="OO339_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5927305.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            346..711
                     /locus_tag="OO339_002729"
     CDS             346..711
                     /locus_tag="OO339_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5927306.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(712..1500)
                     /gene="bioH"
                     /locus_tag="OO339_002730"
     CDS             complement(712..1500)
                     /gene="bioH"
                     /locus_tag="OO339_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5927307.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1538..2221
                     /gene="gntX"
                     /locus_tag="OO339_002731"
     CDS             1538..2221
                     /gene="gntX"
                     /locus_tag="OO339_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5927308.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2280..2855
                     /gene="nfuA"
                     /locus_tag="OO339_002732"
     CDS             2280..2855
                     /gene="nfuA"
                     /locus_tag="OO339_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5927309.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3216..4532
                     /gene="gntT"
                     /locus_tag="OO339_002733"
     CDS             3216..4532
                     /gene="gntT"
                     /locus_tag="OO339_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5927310.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="OO339_002734"
     CDS             complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="OO339_002734"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5927311.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6671..9064)
                     /gene="malP"
                     /locus_tag="OO339_002735"
     CDS             complement(6671..9064)
                     /gene="malP"
                     /locus_tag="OO339_002735"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5927312.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9676..12406
                     /gene="malT"
                     /locus_tag="OO339_002736"
                     /pseudo
     CDS             9676..12406
                     /gene="malT"
                     /locus_tag="OO339_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12449..13465)
                     /gene="rtcA"
                     /locus_tag="OO339_002737"
     CDS             complement(12449..13465)
                     /gene="rtcA"
                     /locus_tag="OO339_002737"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5927313.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13469..14695)
                     /gene="rtcB"
                     /locus_tag="OO339_002738"
     CDS             complement(13469..14695)
                     /gene="rtcB"
                     /locus_tag="OO339_002738"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5927314.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14876..16474
                     /gene="rtcR"
                     /locus_tag="OO339_002739"
     CDS             14876..16474
                     /gene="rtcR"
                     /locus_tag="OO339_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5927315.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16456..17214)
                     /locus_tag="OO339_002740"
     CDS             complement(16456..17214)
                     /locus_tag="OO339_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5927316.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17231..18061)
                     /gene="glpG"
                     /locus_tag="OO339_002741"
     CDS             complement(17231..18061)
                     /gene="glpG"
                     /locus_tag="OO339_002741"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5927317.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18106..18432)
                     /gene="glpE"
                     /locus_tag="OO339_002742"
     CDS             complement(18106..18432)
                     /gene="glpE"
                     /locus_tag="OO339_002742"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5927318.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18622..20127
                     /gene="glpD"
                     /locus_tag="OO339_002743"
     CDS             18622..20127
                     /gene="glpD"
                     /locus_tag="OO339_002743"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5927319.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
BASE COUNT         4729 a         5562 c         5126 g         4765 t
ORIGIN      
        1 gagtcagtag atactctacc ctgcttaagt gctactttgt gctatcttgc atgtaacaca
       61 aagtagcact tagggaactg atatgcagac agtgaaatta cggcaacaag gaggagccat
      121 gatcgttact atcccacgag atcttgccat cgatcttggc tggtcaccgg gcacagagct
      181 taccgtggag aaaaaaggcg atagcgttaa tttgcgcgcc acagagcaca aaccgcgtgg
      241 gcgtcttacc gtcgcgcagt tgttgagcca aatcgatgaa agcgaaatta cggagctaaa
      301 tcagtccaca gaaggatggg ctgaagggaa aaaaggtaat gaagcatggt aaaggcacgg
      361 acgccacatc gtggtgagat ctggtatttt aaccctgatc cggttgccgg gcatgaactt
      421 caggggccac attattgcat tgtggtaacg gacaaaaaac tcaacaatgt tttaaaagtt
      481 gctatgtgct gcccgatttc aacaggggca aatgcagcac gttccacagg ggtgacggtg
      541 aacgtcctcc cccgtgatac gcaaaccggt aacctgcatg gcgttgtact ttgtcaccag
      601 ctaaaagccg tcgatcttat tgcccgtggc gctaaatttc ataccgttgc cgatgaaaaa
      661 ttgattagtg aagttatcag taaactggtg aatttaatcg acccacaata attagctctc
      721 acttaccacc aacaccctct gcttcaacgc caccagcagg tgacaaaact cgaccggatg
      781 cgaaataaac ggcgcatggg ccgctttggc gaagatatat gattcgctgt gaggccaaag
      841 tttatccagc atcggcacca ctttgcgcgg caccagaccg tcgagatagc catacaatcg
      901 caaaaacggc atggacacgt tttgcagcgg ctgacggaga tcgaccgttt tcaggatttc
      961 cagcccgcca ttaagcacgt caacctccgg catcggtaac gccagaacgg ttttcttcaa
     1021 cgcccgcgca tcctggcgcg ccgtttcagt ccccatggtt tgtaacgcca ggaaccgctc
     1081 cactgtacgc tgaaaatcat cactgagttg ctgctgaaat cccgccagca cgtccggttt
     1141 tatccccggc cactcgtcac gagcactaaa acaaggtgac gacgccacgg tgaccagcgc
     1201 ctgaacacgc tcgggatggg ttaacgcaat ctggcttgcc accagcccgc ccagactcca
     1261 gcctaaccaa atggctttat caggtgcctg ttgcagcacg gcttcggcca tatcagcaag
     1321 tgacagcgca ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa ggtgcagcgt
     1381 aaaatgcgag ctaagttcct cgtcaatgca acgccacact tcggcattca gtccccatcc
     1441 gtgcagcagc acaagatgaa cattcccctg acctttggtc tgccaccaga tgttattcat
     1501 ccgctattgt tctcttttga cttacaagga tgaacatatg ctaacagtac cgggattatg
     1561 ctggctatgc cgaatgccac tggcgttagg tcattggggg atttgttcgg tctgttcacg
     1621 cgccacccgc acagataaaa cgttatgccc acaatgtgga ttacccgcca cacactccca
     1681 tcttccctgc ggtcgctgcc tgcaaaaacc gccgccctgg caaagactgg tcacggttgc
     1741 cgactatgcg ccgccgttaa gtccgcttat ccaccagctt aaattttccc ggcgcagtga
     1801 aatcgccagc gccctgtcac gtctgttact attggaagtc ttacacgctc gtcgcaccac
     1861 cgggttgcaa ttgccggatc gcatcatcag cgttccgtta tggcagcggc gtcactggcg
     1921 tcggggattt aatcagagcg atttgctgtg tcagccgtta tcacgctggt tgcactgcca
     1981 atgggatagc gaagccgtca cacgtacacg ggccactgcg acccagcatt ttctcagtgc
     2041 ccggctgcgc aagcgcaacc tgaaaaatgc ctttcgtctt gaattgcccg tgcaaggtcg
     2101 ccatatggtg attgtggatg atgtcgttac caccggaagt accgtcgcag agattgcgca
     2161 gttgctttta cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc gaaccttgta
     2221 gagcctcgat gatgggcgta ttataaccaa ctaaaatagt caactattag gccattacta
     2281 tgatccgtat ttccgatgct gcacaagcgc actttgccaa actgctggca aatcaggaag
     2341 aagggacaca aatccgcgta tttgtgatta accctggcac gcctaacgct gaatgtggtg
     2401 tttcttattg tccgccggat gccgtggaag ccaccgacac tgccctgaaa tttgacctgc
     2461 tgaccgcgta tgttgatgag ttaagcgcac catacctgga agatgcagag atcgactttg
     2521 ttaccgacca gttgggttct cagttaacgc tgaaagcccc gaacgccaaa atgcgtaaag
     2581 tggcagacga tgcaccgctg atggagcgcg tggagtatat gctgcagtcg cagatcaacc
     2641 cacagcttgc tggtcacggt ggtcgcgttt cgctgatgga aatcaccgaa gacggttacg
     2701 ccattctgca atttggcggc ggctgtaacg gttgttccat ggtcgatgtg acgctgaaag
     2761 aagggatcga gaagcagctg ctgaacgaat tcccggagct gaaaggtgtg cgcgatctca
     2821 ccgaacacca gcgcggcgaa cactcctact actaagttat cccctctttt gtagattgcc
     2881 cgatgcgacg ctaaagcgtc atttcgggcc tacgttgaac atgtgccaga cgttggcaca
     2941 gcattgcttt ggcaaatccc tcccttcgtt tccccgcgat aatatgacca acctctcata
     3001 atttaaattt accccgctct ggtgattctc aaacgccaaa tgttacccgt atcattcaca
     3061 tgggtaccaa acatactcct gacatctgac tacaataatt agttttagtg ggtatcagtc
     3121 gtggtgccgc aatatctctg ttcccgattg ggataattag agtttgtcgt cagaaaattg
     3181 acgttaccca taacaaatga aaggccaggt aaatcatgcc attagtcatt gttgctatcg
     3241 gtgtaatctt gttgttgctc ctgatgatcc gcttcaaaat gaacggcttc atcgctctcg
     3301 tcctcgtggc gcttgctgtt ggattaatgc aaggaatgcc gctggataaa gttattggct
     3361 ccatcaaagc cggtgtcggc gggacgctcg gtagccttgc cctgatcatg ggttttggcg
     3421 caatgctggg caaaatgctg gcagactgcg gtggcgcaca acgtatcgcc accacgctga
     3481 ttgccaaatt tggtaaaaag cacatccagt gggcggtggt gttaaccggt tttaccgttg
     3541 gttttgccct gttctatgaa gtgggcttcg tgctgatgct gccgctggtg tttaccatcg
     3601 cggcttccgc gaatattcca ttgctgtatg ttggtgtacc aatggcggct gcactgtctg
     3661 tgacccacgg cttcctgcca ccgcatccgg gcccgactgc gattgccacc attttcaatg
     3721 ccgatatggg taaaaccctg ctgtacggta ctattctggc aatcccgacc gtgattctcg
     3781 ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa gccaatcccg gaaggtctct
     3841 acagcgcgaa aaccttcagc gaagaagaga tgccgagctt tggcgtcagc gtctggacct
     3901 ctctggtgcc ggttgtgctg atggcgatgc gtgcgattgc cgaaatgatc ctgccgaaag
     3961 gtcacgcttt cctgccggta gcggagttcc tcggtgaccc ggtaatggca acgctgattg
     4021 ccgtgctgat tgcgatgttc acctttggtc tgaaccgtgg tcgttcaatg gatcagatta
     4081 acgacacgct ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc atcggtggtg
     4141 gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga caaatacatt gcttccatga
     4201 tgcacgaaac caacatttct ccgctgctga tggcctggtc gattgctgcc gtactgcgta
     4261 tcgcgctggg ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg gcaccgctga
     4321 ttgcaacgac gggtgttagc ccagagctga tggttattgc ggtgggttcc ggtagtgtga
     4381 ttttctctca tgtgaacgat ccgggcttct ggctgttcaa agagtacttt aacctgacta
     4441 tcggcgagac catcaaatcc tggtcgatgc tggaaaccat tatctcggtg tgcggtctgg
     4501 taggctgtct gctgctgaat atggtgattt gataacactg actgccggat gcatcgcatc
     4561 cgacatcaac cgcactctac ttcttcttcg ctgcagctct gcgccgtctg tccaaatcct
     4621 tcagcaactt gttcacgcca tcatcggcaa acatcgactc aagcgttgcg gaaagcttgc
     4681 gtcgccagtt tttatactgg taactggtgc caggaatatt caccggttcg gccatatcca
     4741 gccagtcttc cggctgtagt cctaacagag cactgttact gtcggcaatg tagcgctgca
     4801 aaccacggtt cagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg
     4861 gcagacaacc atatttatgc agtgcatcca gcagcccttg cttcgccagt tcgcgatcct
     4921 gatacagacc gcgcagtacc acttcatccg gatacagccc cagggttttg cccagcgtta
     4981 gatccccgct ctcccagtaa ccacgcagcg ttggcaggtc atgtgtcgcc gcaaccgcca
     5041 tcgactgctc cggatacgct ttcggtgcac ggaacgtctt ctcgtggtcg ttttcgaaat
     5101 agagcacttt gtaagagtac acaccgctgc tgcgcagctt accaacaatc tctaccggta
     5161 cggtaccgag atcttcacca atcaccatac agcgatgacg tttactttcg agtgccagaa
     5221 tcgagagcag atcatccacc ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat
     5281 acggtatcca ccacaaacgc agcatcgaca tcacatggtc aattcgtaat gcgccgcagt
     5341 tttgcatatt ggcacgcaac agctcgataa acggttcata ggcacgcgcg gtgatgatat
     5401 gcgggtccat tggcggtaat ccccagttct gccccaacgg gccgaggata tccggcggcg
     5461 cgccaaccga tgctttcagg caatatagtt cacggtcaca ccaggtttcc gccccacctt
     5521 ccgctacgcc aaccgccaga tcacgataca agccaatcgg catttcatag ccctggctta
     5581 tctcccagca ggcggcaaac tggctgtaag ccagccactg caaccagaga taaaaatcga
     5641 cgtcatcacg atgttcttcg cagaactgac gcacttctgg tgaatccacg ttcttataca
     5701 tctctggcca tgcaggccag ccccagcgca tttcgtcctc tttcacttgc tgggcatgta
     5761 gcgcatcaaa ggcagcctgc cagaacaggc tgtcgccctg ctctgcaaca aactggcgaa
     5821 acgcggccat ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg
     5881 tcatttttag ggcggtaacc gtggagtaat cgacccagtc ggcatcgcgc gcctgttgca
     5941 gcgtctgttg cgtggtcggc aactgccacc aggcctgagc ctcttcgcta agatggaaat
     6001 cttcaacggc gttaacgtcg atataaatca cattcagcca acggcgagaa gacgggctgt
     6061 atgggctggc gctctccgga tttgccggat agagcgcatg aatcgggttc aggccaatga
     6121 acgacccgcc acgttttgcc acatccacca gcatcgcttt gagatcgcca aaatccccaa
     6181 taccccagtt tttttccgat cgcagcgtat aaagctgaac gcaggcaccc cacagctttt
     6241 gtttattcag caaagcctgc ggttcgtaac aacgtttcgg ggcgacaatc acccggcaat
     6301 gcgcgcgctg gtcgtcctgg gtgagtgtta gcgtgtgata accttccggc agcttcgtcg
     6361 gtagattgaa cgctttgccc cccgttacat ggcctttgta ctgcgttcct tcttcggtgg
     6421 tcagctgcca gctatattcg ccgctgccct ccaccaccat cggcattttt ttgccgctgg
     6481 tataaaccat gacattcggg actggcgtta ccgccacttt cgtggcggta cgttgatgca
     6541 tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat
     6601 tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt atccagacgt ttgctttcca
     6661 tcgagcttcc ttagcgtttt gcctgccaga tacgagcctg ataatcgcga atagagcgat
     6721 ccgagctaaa cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct
     6781 ggtcgcggta cagcacatcc acctgctttt gtgcctcaac ataggctgcg aaatccgcca
     6841 tcaccagata cggatcgccg ccctgtttgc cgatactgtg cagcatctgg tcgaaggcat
     6901 gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt caatactgcg tccagcacct
     6961 tatctttctt ccgccatttc accgggtcgt agcctttggc cagaatagcc ttcacttgtt
     7021 ccacggtatg accaaaaata aagatatttt cttcaccgac tttctcggcg atttcaacgt
     7081 tcgcaccatc cagcgtaccg acagtaagcg caccattgag cgccagtttc atattgccgg
     7141 taccggaagc ttctttacct gcagtcgaaa tttgttcgga gatatccgcc gccgggatca
     7201 gtttttccgc cgccgaaacg caataatccg gcaggaacac caccttcaac ttatcgccaa
     7261 ccagtggatc gttgttgatc acgtcagcca ctttgttgat cgcaaagata atattcttcg
     7321 ccaggtagta gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt acgcgatcag
     7381 cctgcaggtt ttcacgaatt tctttgtaca acgccagaat atgcagcaga ttcaggtgct
     7441 ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt ggattgatct
     7501 caataccggt acgaactttc acaaactccg ccagacagac tttattcgcc tgcttgatct
     7561 cgcgatattg ctgacggaat ttcgcatcat cagcgaattt ttccagattg atcagctgat
     7621 cgagatcgtt agcccactct ttttgcagtg atttatccaa cagagccgcc agtgcagggt
     7681 tgcactgttt gatccagcga cgtggggtaa taccgttggt aacgttatgg aatttgttcg
     7741 gccatagctg gtgatattcc gggaacagat ctttcaccac cagatccgag tgcagcgccg
     7801 caacaccgtt caccgcgaaa ccgccaacca cacacaggtt cgccatatgc acttgtttat
     7861 cgtgcaccac cgccagtttg gcccacactt tttcatcgcc cggccaggtt ttctccacca
     7921 gcgttttaaa gcgagtatta atttcgttaa taatctgcat atggcgcggc agtaagcctt
     7981 tcaccagttt cacatcccag cgttccagcg cttctggcat cagggtatgg ttggtgtaag
     8041 cgaaagtttt gctggtgatg gcccaggcgt catcccagct catctggtgc tcatcggtca
     8101 gcacgcgcag cagttctgga atcgcgatag ttggatgggt atcgttcagc tgaataactt
     8161 cgtaatccgc cagttcgtgc agtttacgcc ccgccagatg atggcgacgc aaaatatccg
     8221 ctaccgaaca ggcacactgg aagtattgct gcatcaggcg cagcttttta ccggcagtat
     8281 ggttgtcgtt tggatagaga actttggtca gtttttccgc attgatgcct tgctgttcgg
     8341 cgcgcaggaa atcaccgtcg ttaaatttag ttagatcaaa cggatgcgca tgcgtcgcct
     8401 gccacagacg cagcggctgc gccacgccat tacgatagcc gacaacgggg agatcccacg
     8461 cttgaccggt aatggtaaac tccggctccc agcgtccgtc tttcatcact ttaccgccaa
     8521 tccctacctg cacatccagt gcttcgttgt ggcggaacca cgggtagtta ctgcgatgcc
     8581 agtcatccgg cgcttcaacc tgtttgccat cgacaaaaga ctggcggaac aaaccatatt
     8641 gatagttcag accgtaaccc gtcgcagact gaccgacagt tgccattgag tcgaggaagc
     8701 acgccgccag acgtcccaga ccaccgttac ccagcgccgg gtcgatctct tcttccagca
     8761 ggtcagtcag gttgatgtca taagccttca acgaatcctg tacatcctga taccagccga
     8821 gattcaacag gttgttgccc gtcagacgac caatcaaaaa ctccattgag atgtagttaa
     8881 catgtcgctg attcgccacc ggtttggcga atggctgagc acgcagcatt tcggccagtg
     8941 cttcactcac tgccagccac cactggcgag gagtcatttc agccgcagaa tttaagccat
     9001 aacgctgcca ctgacgtgaa agcacttcct gaaattgctt atcgttaaaa ataggttgtg
     9061 acataggagt tccacttttc ttagattttc aacacaacgt tatcgctagt ttgccaggct
     9121 cgatgttgac cttcctcatc ctgcggggga ttaggcaggg aggagttgcg gggatgagca
     9181 aggaaatgtg atctcgacca cttaaagcta gtgcaaacca caggattagc agcaaatcaa
     9241 tgccatacag cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa
     9301 aaagcggaag gtaactctat aaattaagta aaggagtgaa acagtttcac aagtaaaata
     9361 tccagtgtgc tccatctcat tcttaataga tttattaaga tcatcttttt agatggcact
     9421 ttcatcagga atgaagaaga aacccttgct taaatgaatc tgatgaacat aagggaaacc
     9481 agtattcacg ctggatcagc gtcgttttag gtgagttgtt aataaagatt tggaattgtg
     9541 acacagtgca aattcagaca cataaaaaaa cgtcatcgct tgcattagaa aggtttctgg
     9601 ccgaccttat aaccattaat tacgaagcgc aaaaaaaata atatttcctc attttccaca
     9661 gtgaagtgat taactatgct gattccgtca aaactaagtc gtccggttcg actcgaccat
     9721 accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt ccggctggcg
     9781 ctgatcacga gtcctgcggg ctacggaaag accaccctca tttcccagtg ggcggcaggc
     9841 aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga gcgtttcgcc
     9901 agctatctca ttgccgccgt gcagcaggca accaacggtc actgtgcgat atgtgagacg
     9961 atggagcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct tttcattgag
    10021 ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct gatcactaat
    10081 ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa tctcaccctg
    10141 gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt tcgtgaccaa
    10201 ctgctggaaa ttggcagtca gcaactggca tttacccatc aggaagcgaa gcagtttttt
    10261 gattgccgtc tgtcatcgcc gattgaagcc gcagaaagca gtcggatttg cgatgacgtt
    10321 tccggttggg cgacggcact atagctaatc gccctctccg cccggcagaa tacccactca
    10381 gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc ggattatctg
    10441 gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc attttctgtt gaaaagcgcc
    10501 attttgcgct caatgaacga tgccctcatc acccgtgtga ccggcgaaga aaacgggcaa
    10561 atgcgtctcg aagagattga gcgtcagggg ctgtttttac agcggatgga tgataccggc
    10621 gagtggttct gctatcaccc gctgtttggc aacttcctgc gccagcgctg ccagtgggaa
    10681 ctggcggcgg agctgccagt gggaactggc ggcggagctg ccggaaatcc accgtgccgc
    10741 cgcagaaagc tggatggccc agggatttcc cagcgaagcg attcatcatg cgctggcggc
    10801 aggcgatgcg ctgatgctgc gcgatattct gcttaatcac gcctggagtt tgttcaacca
    10861 tagcgaactg tcgctgctgg aagagtcgct taaggccctg ccgtgggaca gtttgctgga
    10921 aaatccgcag ttagtgttat tgcaggcgtg gctaatgcaa agccaacatc gctatggcga
    10981 agttaacacc ctgctagccc gtgctgaaca tgaaatcaag gacatcagag aaggcaccat
    11041 gcatgcagaa tttaacgctc tgcgcgccca ggtggcgatt aacgatggta atcaggatga
    11101 agcggaacgg ctggcaaaac tggcactgga agagctgccg ccgggctggt tctatagccg
    11161 cattgtggca acctcggtgc tgggtgaagt gctgcactgc aaaggcgaat tgacccgctc
    11221 actggcgcta gtgcagcaaa ccgaacagat ggcacgccag cacgacgtct ggcactacgc
    11281 cttatggagt ttaattcagc aaagtgaaat tctgtttgcc caagggttcc tgcaaaccgc
    11341 gtgggaaacg caggaaaaag cgttccagct gatcaacgag cagcatctgg aacagctgcc
    11401 aatgcatgag tttctggtgc gcatccgagc acaactgtta tgggcctggg cacggctgga
    11461 tgaagccgaa gcgtcggcgc gtagcgggat tgaagtcttg tcgtcttatc agccacagca
    11521 acagcttcag tgcctggcaa tgttgattca atgctcgctg gcccgtggtg atttagataa
    11581 cgcccgtagc cagttgaacc gtctggaaaa cctgctgggg aatggcaaat atcacagcga
    11641 ctggatctct aacgccaaca aagtccgggt gatttactgg caaatgaccg gcgataaagc
    11701 cgccgctgcc aactggttgc gtcatacggc taaaccggag tttgcgaaca accacttcct
    11761 gcaaggtcaa tggcgcaaca ttgcccgcgc acaaatcttg ctgggcgagt ttgaacctgc
    11821 ggaaattgtt ctcgaagaac tcaatgaaaa tgcccgcagt ctgcggttga tgagcgatct
    11881 caaccgtaac ctgttgctgc ttaatcaact gtactggcag gccggacgta aaagtgacgc
    11941 ccagcgcgtg ttgctggacg cattaaaact ggcgaatcgc accggattta tcagtcattt
    12001 tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag ctgattcaac ttaatacgct
    12061 gccggaactg gaacagcatc gcgcgcagcg tattctgcga gaaatcaatc aacatcatcg
    12121 gcataaattc gcccatttcg atgagaattt cgttgaacgt ctgctaaatc atccggaagt
    12181 gccagaacta atccgcacca gcccgttgac ccaacgtgaa tggcaggtcc tggggctgat
    12241 ctactccggt tacagcaatg aacaaattgc gggcgaactg gaagtggcgg caaccaccat
    12301 caagacgcat atccgcaatc tgtaccaaaa actcggcgtc gcccaccgtc aggctgccgt
    12361 gcagcacgcg cagaaactgc tgaagatgat gggttatggg gtgtgagttt agccggataa
    12421 tgcattggat ccggtttaca tctttacatc attcaatact cacccgcgtt acgccatctg
    12481 tttctatcaa accaaaccgc accggcaaga aacgctccac caccgcgata ttggtcagca
    12541 gatggcatga aggatgggcg accgtaaatt ctcccgcgcc cgccagcgcc atcggcagta
    12601 ccagttggtc ggcgagatat tcccccaccg ccgctgggct tgccaggtag cgtttcacct
    12661 ctttcactaa ctgcgcggct accacctcgg cactgacgcg cttttcaccg acgacgaaaa
    12721 agcgttcagt gatattttca ctttcgactt ccagcgagac ggtattaccc ggcccctggt
    12781 cgcgcggcag gttatgaata ttctgttcat gcagggaaaa aatccccgcc agtgtagcga
    12841 tttcacgctc agcaacatgg cgcggcacac cagctaatag aacttctcca cgcatctgca
    12901 caatgttccc gcgctcgcca agttgcaagg tgctaaacga tgccacgggc gagacttccg
    12961 ttgccaccac accgcctccg gcaggataaa aaccgtgacg taacagcgtg gtttgctgat
    13021 gaattcctat tttcgccagc agcggctcca gcacccggcg gataaaatcc gcaggcggcg
    13081 ccgacgggtt atcggtgccg ccgctcactt caacacgcga aggtccatcg gcaaaccaca
    13141 gcgcgggcag caccgtttgc agcaccagcg tacaacttcc ggcgctaccg atagcaaagc
    13201 ggtaatcgcc gccgcgcacg gtgccaggcc ggaagaccag acgctgcgat cccagctccg
    13261 cgccttccac cgttgcccta caaatttccg cagccgcttt taccgcggtc agatgctggc
    13321 gcagcagccc cggtttcgcc cgtccggctc gaatgccagt gatggtaaat ggctggccgg
    13381 ttatcatcga caggctcagc gccgagcgca ggatctgccc gccgccttcg ccctgtgcgc
    13441 catccagcgc aatcatcctt ttcatcattt atccttttac gcacaccacc tgacgcaggg
    13501 tgtagataac ttccaccaga tcgctttgtg ccgccatcac cgcatcaata tctttatacg
    13561 ccatcgggat ttcgtcgatc acttcggcat ctttacggca ttccacatgc gcggtggcac
    13621 gaatttggtc ttccacgctg aacagttttt tcgctttagt tcggctcatt acccgcccgg
    13681 caccgtggct gcacgaacag aacgactctt catttcccag cccacggacg ataaagcttt
    13741 ttgctcccat cgaaccggga ataattccat attgaccagc acgcgcagac accgcacctt
    13801 tacgcgtcac gtagatctct tcaccaaaga gctgttcttt ttgcacatag ttgtggtgac
    13861 agttgatctc ttccatcgcc agcgtttgtg gctgtctgac cgttttctgc gtaatgctct
    13921 gcaacgccgt taccacattt tccatcatcg catcgcggtt aaggctggcg aaaagctgcg
    13981 cccaggccac ggctcttagg taatcatcaa agtattccgt accttccata aagtacgcca
    14041 gatcacgcga cggcaacgtc tcaagttggt cctgcatctc tttttgcgcc agatcgataa
    14101 agtaagtccc gatggcgtta ccaattccgc gtgaaccgga gtgcagcata atccacacct
    14161 gctccgactc atcaaggcag atttcaataa agtggttacc cgttcccagc gttcccaggt
    14221 gtttatagtt gttagtgtta aggaaacgcg gatatttttg cgttaaccac tgataaccgg
    14281 cctccagctc agcccatttc gcatcgacgt taaaaggcgg gttttcccat gcgcctttat
    14341 cacgtttaca acgcccggta gtacgcccgt gtggtacggc cgtttcaatc gcttgacgca
    14401 gctccgccag gttttcaggc aggtctgccg ccgttaacgc ggtacgcagc gcgttcattc
    14461 cacagccaat atccacgccc accgccgccg gaataatcgc ccctttggtc gggatcacgc
    14521 taccaatggt ggaaccttta cccaggtgta catcaggcat taccgcaata tgtttgaaaa
    14581 taaacggcat cttcgccgta ttaataagtt gctgacgcgc atcggcttct accggcacgc
    14641 ctttggtcca catttttacc ggggcatttt cagtggtcag taattcgtaa ttcattttgt
    14701 ttttctcttt tcgttgtttg cggtcctgat aattgcaacc gtcgtgccag aaaattaaat
    14761 aagcagatta atttattgtt tctaaaaaga ttatatcttt atatccgtaa acggagattt
    14821 cccgcaaagc cgatttaccg ataatgaaat atcgcctttt ataaggatat ctaagatgcg
    14881 taaaacagtg gcttttggct ttgtcggtac cgtgctggat tatgccggac gcggcagtca
    14941 gcgctggtca aaatggcgtc cgtcactctg tatataccag caagaatcgt tggtcatcga
    15001 tcgactggaa ttattgcacg atgcccgctc gcgctcgcta tttgaaacgc ttaaacgcga
    15061 tatcgccagc gtttcgccag aaacagaagt ggtgggcgtt gagattgaac tgcataaccc
    15121 gtgggatttc gaagaggttt acgcctgcct gcatgatttc gcccgtggtt acgagtttca
    15181 gccagaaaaa gaagactatt taattcacat caccaccggc acccacgtcg cgcagatttg
    15241 ctggtttctg ctggcggaag cgcgttatct acccgcccgg ttgatacaat cttcaactcc
    15301 gcgcaaaaaa gaacagccgc gcggcccagg tgaagtgacg attatcgatc tcgatttaag
    15361 ccgttataac gccatcgcca gccgctttgc cgaggaacgg cagcaaacgc tcgatttcct
    15421 caaatccggt attgccacgc gtaaccccca cttcaaccgc atgattgagc agatcgaaaa
    15481 agtggcgatt aaatcccgcg cgccgattct gcttaacggc ccgaccggcg caggtaagtc
    15541 atttctggcg cgacgcatct tcgagttaaa acaggcacgg catcagttta gcggcgcgtt
    15601 tgtggaagtg aactgcgcca ccctgcgcgg cgataccgcg atgtcgacgc tgtttggtca
    15661 tgtgaaaggc gcgtttaccg gggcgcggga atctcgtgaa ggattattac gcagcgccaa
    15721 cggcggaatg ttgtttcttg atgagattgg cgaactgggc gcagacgaac aggcaatgct
    15781 gctgaaagct attgaagaga aaacctttta cccgtttggc agcgatcgcc aggtgagcag
    15841 tgattttcag cttatcgccg gaacggtgcg cgatttgcgc cagctggttg ccgaaggcaa
    15901 attccgcgaa gatctgtacg cgcggatcaa tctctggacc ttcaccctgc cgggtctacg
    15961 ccagcgccag gaagatattg aaccgaacct ggattatgaa gtggagcgcc acgccacact
    16021 cactggtgac agcgtgcgtt ttaacaccga agcgcggcgt gcctggctgg cctttgcaac
    16081 atctccccag gcaacatggc gcggtaactt tcgcgaactt tctgccagcg tcacgcggat
    16141 ggccaccttt gccactagcg gacgcatcac tctggacgtg gtggaagatg agataaaccg
    16201 tctgcgctat aactggcagg agagccgccc ctccgcgctt acggcgttgc tgggcgcaga
    16261 ggcagaaaac atcgatctct tcgaccgtat gcaactggaa cacgttatcg ctatctgccg
    16321 ccaggcaaag tcgctttctg ctgccggacg acagcttttt gatgtttcgc gccagggcaa
    16381 agccagcgtc aatgacgcgg atcggctacg caaatacctg gcgcgttttg gtctgacgtg
    16441 ggaagccgtg caggatcagc acagctccag ttgaatatgg tggtccgtca gcacctgcat
    16501 cacgcttact ggcggcgggg cgtcggtgta gacggcatca accatgctga tgctccccat
    16561 attgaccatc gcattacggc caaattttga atggtcaacc accagcataa cgtggcgcga
    16621 gttctcaata atggcgcgct tggtacgaac ttcgtggtaa tcgaactcca gcagcgagcc
    16681 gtcgctgtcg atgccgctga tccccagaat gccgaaatca aggcggaact gggagataaa
    16741 atcgagcgtc gcttcgccaa tgatcccgcc atcgcggcta cgtaattcgc caccggcgag
    16801 aataatgcgg aaatcttctt ttaccatcaa cgtgttagca acgttgagat tgttggtgac
    16861 aatgcgcaaa ttgctgtgat tgagcagcgc gtgcgccacc gcttccggcg tggtgccgat
    16921 atcgataaac agcgtcgagc cattggggat ttgctccgcc actttgcggg cgatgcgctc
    16981 tttttcttcg gtctgggtgg ctttacgatc gtgccacggc gtgttaaccg aactggaagg
    17041 cagcgccgcg ccgccatgat ggcgcaggat caggttttgc tctgccagct cgttgaggtc
    17101 gcggcgaata gtctgcgggc tgacggagaa gtgctccacc agttcctcgg tactgacata
    17161 accctgctgt ttaaccagtt cgataatacc gttgtgacgt tgtgtttgtt tcatttataa
    17221 atccctggaa ttattttcgt tttcgcgcat tgagcgaatc aacaaaagcc atcgctaaac
    17281 ccacggctaa tccggcgatg tgtgctccgt tcgccatcga catcccaaac aaatcaaacc
    17341 atccggcgac aatccagatc agcgcaaaga taattaaccc acgttgcagg taaatgccac
    17401 tttgcggatc gcgttcgcca cgtagccaga cgtagcccat cagcgcatac accacgccag
    17461 aaagcccgcc aaaccacggc ccgctgaatt tttgctgcat atagccgctt aacagggcgc
    17521 tgataagcgt aatgacaatt agcttaccgc taccgaggcg tttttccacc gcgccgccga
    17581 gataccacca ccaaagcagg ttaaagagga tatgcatcag cgagaagtgc attaacgcgt
    17641 gggtgaagta acgccagaac tcaaatttca gtgttggatc gaatggccag gccagccata
    17701 acatcacttc ctgatcgccg agaatttgca tggcaataaa caccaccacg caggcgatca
    17761 tcatcaccca ggtcaccgga cctgcgcgtt cacgcaaggc agcaaagaaa ggataacggc
    17821 gataatgcag gccactgccg gtatggcctg ccagccagct ggccgccaga taacgcggat
    17881 ctgccgggtt ttcgagaaaa cgcgccagct ccgcccgcac gcgctcggcc tgggactcat
    17941 ccgccagcca gacatcgctt tggttatgtt gttgaatcgt gaggataaca ccctgcgtcg
    18001 ccatgtaatc aacaaacgcc tgcgccacgc gggggttagc aaaagaggta atcatcaaca
    18061 tcgttgctgt cgcttattcc acacaaaagg ggacagtata aagcgttacg cgccgtacgc
    18121 cacctctgcg ggaaactgtc gctgccaggc ttcaaagccg ccgtcaatgc tatagaccac
    18181 atcgtagccc tgttgcagca gatactgcgc cgcccctttg ctgctattgc cgtgataaca
    18241 catcaccatc accggagtgt caaagtcgtt atcacgcata aacgcgccca gcgtgtcgtt
    18301 ggttaaatgg aaagcctgca ccgcatgccc catagcgaaa ctctgtggat cgcgaatatc
    18361 gaccagcacc gcctcttttt cctgcaactt ctggtgcgcg tcggcaacgt taatacattc
    18421 gaactgatcc atgcgtctct ctttctttac aaacaagtgg gcaaatttac cgcacagttt
    18481 acgtcgaagc ggcagataaa cgccataatg ttatacatat cactctaaaa tgttttttca
    18541 atgttaccca aagcgcgatt ctttgctaat atgttcgata acgaacattt atgagcttta
    18601 acgaaagtga atgagggcag catggaaacc aaagatctga ttgtgatagg tggcggcatc
    18661 aatggtgctg gtatcgcggc agacgccgct ggacgcggtt tatccgtgct gatgctggag
    18721 gcgcaggatc tcgcttgcgc gacctcttcc gccagttcaa aactcattca cggtggcctg
    18781 cgctaccttg agcactatga attccgcctg gtcagcgagg cgctggctga acgtgaagtg
    18841 ctgctgaaaa tggctccgca tatcgccttc ccgatgcgtt ttcgcctgcc acatcgtccg
    18901 catctgcgcc cggcgtggat gattcgcatt ggtctgttta tgtacgatca tctgggtaaa
    18961 cgcaccagcc tgccgggatc aactggtttg cgttttggcg caaattcagt gttaaaaccg
    19021 gaaattaagc gcggattcga atattctgac tgttgggtag acgacgcccg tctggtactc
    19081 gccaacgccc agatggtggt gcgtaaaggc ggcgaagtgc ttactcggac tcgcgccacc
    19141 tctgctcgcc gcgaaaacgg cctgtggatt gtggaagcgg aagatatcga taccggcaaa
    19201 aaatatagct ggcaagcgcg cggcctggtt aacgccaccg gcccgtgggt gaaacagttc
    19261 ttcgacgagg ggatgcatct gccttcgcct tatggcattc gcctgatcaa aggcagccat
    19321 attgtggtgc cgcgcgtgca tacccaaaag caggcctaca ttctgcaaaa cgaagataaa
    19381 cgcattgtgt tcgtgatccc gtggatggac gagttttcca tcatcggcac caccgatgtg
    19441 gaatacaaag gtgatccgaa agcagtgaag attgaagaga gtgaaatcaa ttacctgctg
    19501 aaagtgtata acgcgcactt taaaaagcag ctaagccgtg acgatatcgt ctggacctac
    19561 tccggtgtgc gtccgctgtg tgatgatgag tccgactcgc cgcaagccat tacccgtgat
    19621 tacacccttg atatccatga tgaaaacggc aaagcaccgc tgctgtcggt attcggcggt
    19681 aagctgacca cctaccgaaa actggcagaa catgcgctgg aaaaactgac gccgtattat
    19741 cagggtattg gcccggcgtg gacgaaagag agcgtgctgc cgggtggtgc cattgaaggc
    19801 gatcgcgacg attacgccgc tcgcctgcgc cgccgttacc cgttcctgac tgaatcgctg
    19861 gcacgtcact acacgcgcac ttacggcagc aacagcgagc tgctgctcgg caatgcggga
    19921 acggtaagcg atctcgggga agatttcggt catgagttct acgaagcgga actgaaatac
    19981 ctggtggatc acgaatgggt ccgccgcacc gacgatgccc tgtggcgtcg cacaaaacaa
    20041 ggcatgtggc tgaatgcgga tcaacaatct cgtgtgagtc agtggctggt ggaatatacg
    20101 cagcagaagt tgtcgctggc gtcgtaaatt aacgtaaggt gatcaggtca gatttcaatc
    20161 tggcctgaga ctgatgacaa ac
//
LOCUS       DAJTRH010000071        20084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTRH010000071 DAJTRH010000000
VERSION     DAJTRH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="OO339_002744"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="OO339_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5927320.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO339_002745"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO339_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5927321.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="OO339_002746"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="OO339_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="OO339_002747"
     CDS             1631..2341
                     /locus_tag="OO339_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5927322.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO339_002748"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO339_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5927323.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="OO339_002749"
     CDS             complement(3469..4119)
                     /locus_tag="OO339_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5927324.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6480
                     /locus_tag="OO339_002750"
     CDS             4282..6480
                     /locus_tag="OO339_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5927325.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="OO339_002751"
     CDS             complement(6449..6889)
                     /locus_tag="OO339_002751"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5927326.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="OO339_002752"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="OO339_002752"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5927327.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="OO339_002753"
     CDS             7607..8305
                     /locus_tag="OO339_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5927328.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="OO339_002754"
     CDS             8534..9742
                     /locus_tag="OO339_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5927329.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="OO339_002755"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="OO339_002755"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5927330.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="OO339_002756"
     tRNA            11849..11925
                     /locus_tag="OO339_002756"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="OO339_002757"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="OO339_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5927331.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="OO339_002758"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="OO339_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5927332.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="OO339_002759"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="OO339_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5927333.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="OO339_002760"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="OO339_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5927334.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="OO339_002761"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="OO339_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5927335.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="OO339_002762"
     CDS             complement(18540..19811)
                     /locus_tag="OO339_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5927336.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4812 a         4974 c         5275 g         5023 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agca
//
LOCUS       DAJTRH010000072        20078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTRH010000072 DAJTRH010000000
VERSION     DAJTRH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..579
                     /locus_tag="OO339_002763"
     CDS             <1..579
                     /locus_tag="OO339_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5927337.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO339_002764"
     CDS             597..1235
                     /locus_tag="OO339_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5927338.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO339_002765"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO339_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5927339.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO339_002766"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO339_002766"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5927340.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO339_002767"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO339_002767"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5927341.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO339_002768"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO339_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5927342.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO339_002769"
     CDS             complement(5552..6613)
                     /locus_tag="OO339_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5927343.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO339_002770"
     CDS             complement(6610..8007)
                     /locus_tag="OO339_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5927344.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO339_002771"
     CDS             8163..9161
                     /locus_tag="OO339_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5927345.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO339_002772"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO339_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO339_002773"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO339_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO339_002774"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO339_002774"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5927346.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO339_002775"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO339_002775"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5927347.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO339_002776"
     CDS             complement(14232..14855)
                     /locus_tag="OO339_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5927348.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO339_002777"
     CDS             complement(14852..15274)
                     /locus_tag="OO339_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5927349.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO339_002778"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO339_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO339_002779"
     CDS             complement(16089..17141)
                     /locus_tag="OO339_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5927350.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO339_002780"
     CDS             complement(17172..18014)
                     /locus_tag="OO339_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5927351.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO339_002781"
     CDS             complement(18001..18882)
                     /locus_tag="OO339_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5927352.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20078)
                     /locus_tag="OO339_002782"
                     /pseudo
     CDS             complement(18903..20078)
                     /locus_tag="OO339_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT         4872 a         5517 c         4920 g         4769 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccga
//
LOCUS       DAJTRH010000073        19997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTRH010000073 DAJTRH010000000
VERSION     DAJTRH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            271..330
                     /gene="ibsE"
                     /locus_tag="OO339_002783"
     CDS             271..330
                     /gene="ibsE"
                     /locus_tag="OO339_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5927353.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(450..1883)
                     /gene="hldE"
                     /locus_tag="OO339_002784"
     CDS             complement(450..1883)
                     /gene="hldE"
                     /locus_tag="OO339_002784"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5927354.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1931..4771)
                     /gene="glnE"
                     /locus_tag="OO339_002785"
     CDS             complement(1931..4771)
                     /gene="glnE"
                     /locus_tag="OO339_002785"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5927355.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4794..6095)
                     /gene="ygiF"
                     /locus_tag="OO339_002786"
     CDS             complement(4794..6095)
                     /gene="ygiF"
                     /locus_tag="OO339_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5927356.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6337..6957
                     /locus_tag="OO339_002787"
     CDS             6337..6957
                     /locus_tag="OO339_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5927357.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            7021..8259
                     /gene="cca"
                     /locus_tag="OO339_002788"
     CDS             7021..8259
                     /gene="cca"
                     /locus_tag="OO339_002788"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5927358.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8422..9243)
                     /gene="bacA"
                     /locus_tag="OO339_002789"
     CDS             complement(8422..9243)
                     /gene="bacA"
                     /locus_tag="OO339_002789"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5927359.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9334..9702)
                     /gene="folB"
                     /locus_tag="OO339_002790"
     CDS             complement(9334..9702)
                     /gene="folB"
                     /locus_tag="OO339_002790"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5927360.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9807..10424
                     /gene="plsY"
                     /locus_tag="OO339_002791"
     CDS             9807..10424
                     /gene="plsY"
                     /locus_tag="OO339_002791"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5927361.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10437..11368)
                     /gene="ttdR"
                     /locus_tag="OO339_002792"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10437..11368)
                     /gene="ttdR"
                     /locus_tag="OO339_002792"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11575..12418
                     /gene="ttdA"
                     /locus_tag="OO339_002793"
                     /pseudo
     CDS             11575..12418
                     /gene="ttdA"
                     /locus_tag="OO339_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12415..13020
                     /gene="ttdB"
                     /locus_tag="OO339_002794"
     CDS             12415..13020
                     /gene="ttdB"
                     /locus_tag="OO339_002794"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5927362.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            13068..14531
                     /gene="ttdT"
                     /locus_tag="OO339_002795"
     CDS             13068..14531
                     /gene="ttdT"
                     /locus_tag="OO339_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5927363.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14574..15587)
                     /gene="tsaD"
                     /locus_tag="OO339_002796"
     CDS             complement(14574..15587)
                     /gene="tsaD"
                     /locus_tag="OO339_002796"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5927364.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15825..16040
                     /gene="rpsU"
                     /locus_tag="OO339_002797"
     CDS             15825..16040
                     /gene="rpsU"
                     /locus_tag="OO339_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5927365.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            16151..17896
                     /gene="dnaG"
                     /locus_tag="OO339_002798"
     CDS             16151..17896
                     /gene="dnaG"
                     /locus_tag="OO339_002798"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5927366.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            18091..19932
                     /gene="rpoD"
                     /locus_tag="OO339_002799"
     CDS             18091..19932
                     /gene="rpoD"
                     /locus_tag="OO339_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5927367.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4765 a         5569 c         5119 g         4544 t
ORIGIN      
        1 actttaggtt gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca
       61 aaaagccggg gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg
      121 cagcgttttg cgtgaccgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg
      181 gtccggcgct catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc
      241 gaggaagggg ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg
      301 ttgttactgt taagtttccc gacttactaa caactgatca gaggggggag aaatcctccc
      361 ttacccttgt tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta
      421 cgaagcgaga tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg
      481 atgatgttag tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg
      541 gcccagactt ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt
      601 aaatctggca ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc
      661 cagtcgaccg cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg
      721 cgagaatccc ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcaagcgg
      781 tcacccagtt tgcgggcatt tgccagatag aagacgtgtc cggcgtgcag gatgtcaaag
      841 acgccattgg tcatcaccac tttttcaccg cgtttacgcg ctgccgctac ggccagcttc
      901 agttcctctt cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc
      961 agctcgatcg gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca
     1021 ttggcaaaga agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg
     1081 ccaatcaccg tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata
     1141 tgcagcggcg ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac
     1201 agagccgaga gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg
     1261 gtcttacatt taccgacaac agcttcaaat tccgagagat tcggtgtcag cagcgtagcg
     1321 ccgcggtagc gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta
     1381 cgcgccagtt ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc
     1441 accagcgcgc caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca
     1501 acaccttcga aaccttcttc aaaatccaga cggatcagct gttggttgcg ggaaagtacc
     1561 cgtaatttgg tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg
     1621 tcggccagag atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg
     1681 cgtgcattag caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt
     1741 tcttcgatgg tattcacttt aaccacgggc accggcgctt ccggcgagat acgactggtg
     1801 gggccgtacc agtaacgatc cagcatcaca tcaccaacca ccatcactcc tgcacgttca
     1861 aactctggca gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata
     1921 ataccatact tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct
     1981 ctgcggtgaa gcaatcctcc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa
     2041 gttcatcgcg cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa
     2101 tgtcgttttg cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg
     2161 gtttttcatg agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc
     2221 cgccttcatc ggctttgata tcaaagcgat tacgatgttt attgccgaga tgagcgcgca
     2281 ttttctcgcg catttcccgc acttccgttt gcagagtttt accttctcgc ggcagtgtca
     2341 taatctcacg acgcaccgcg tcaaagtgcg cagtgagctg cggatcgccg tacactacac
     2401 gcgcacgcac cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa
     2461 atgcttctgc ggatgtcacc agcattcccg ccgccccgga cggacgcagt cgagcatcca
     2521 cttcatacaa aatgccggaa gaggtacgcg tactgaacag atgcataatg cgttgcgcca
     2581 gacgcagata aaactgccgc ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg
     2641 ggcaatcatg gaggaagata aggtcaagat cggaactgta gcctaactcc cagccgccca
     2701 gcttgccgta gccgaccacc gcaaaaccgc gcccttcacg ttcgttgagg tgatttggct
     2761 taccgtagcg ggccaccatt tgaccccacg cctgctgaac gacggcatct atcatagctt
     2821 ctgccagcca ggttaagtga tcgctcactt tcattaccgg tagcgtaccg gcgatatccg
     2881 ctgccgcaat gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgtt
     2941 cttcgtcatc ttccggcacg cgcagcaaat actggcgcag ttcatcacgg taggcatcgg
     3001 tcgccgtcgg ctgataaagg gtatttggat cgagcaattc atccagcaat aatggataac
     3061 gcgctagctg gctggcaatc atcggcgatg cggcacacag agaaatcaaa tgtttaagcg
     3121 ccgcagggaa ttcactgagc aattctaaat aggttgtgcg ggtaacaatc cccaccagca
     3181 aggcggtaat gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca
     3241 gatgtggcat cagatggtcg agcacctgac gtcctcgcgg cccgatagta cgcttatcca
     3301 gctctttgcg gaaatcagca atcatcgtta ggacctgttt gcgatcatcc tcgctaagat
     3361 gcgccagcac tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact
     3421 gttccgacag cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca
     3481 cccggcgcac attggtcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa
     3541 agtccatcgc ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtttgggttt
     3601 gttcgtcgtt aatgctttgc agcaggtttt ccagacgccg caggaacaga tatgccactc
     3661 gcaattgttc agcatcgttt tcagaaagca gatgcagcgc ggcaatggcg ctgagcgttg
     3721 gcagtaaaga gcgcgactgc agcaacggtt cgcgcccacc gcgaatgagc tggaacacct
     3781 gaacgataaa ttcaatttcg cgaatgccgc ctgcgccgag tttgatattg tcggtcaaac
     3841 cacgtcgacg cacttcacgg gcaatcatcc ctttcatgtt gcgcagcgac tgaatcacgc
     3901 tgaaatcgat gtaacgacgg aaaacaaacg ggcgcagcat cgcacgcaac tcgttagcat
     3961 agacgccttc gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc
     4021 gtccctgctc ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt
     4081 cgccaaacgg acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat
     4141 ccagcacttt aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc
     4201 ggcgtccgcc ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg
     4261 agaaattcag ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt
     4321 cgccctgcgc attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac
     4381 gcgccgcaac aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag
     4441 taaccagtgc cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc
     4501 gcagctcgcg cattaacccg gcttcgtcgc tcacattact gagcgcctcc tgcaaccaca
     4561 cgctgtaatg ttgccattcg tcagcctgtg gcggttggct ttccagttcc gtcagccact
     4621 ctggatgcgc aatcacgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct
     4681 gtgcactaag tgattcctcg gctaaaagct ctggcagccg ctcaacaacg gtctgccagt
     4741 actgctgtaa cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt
     4801 tttccgctgt gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgttcaatt
     4861 tcaatgcgtt gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggct
     4921 tgcgcgacga caggatcata gtaacccgcc agcaacagta ttgagtcaat atcacgcatc
     4981 aggcgtggca actggtcacg gtagcgatcg cctaacggct ggcagaaaac gcttttcagt
     5041 tcagcggcat ggcgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctaattttg
     5101 ccctgcgctt tggcatctaa aaacggctgc catgctttgc ttaccagcca ttcggtcaac
     5161 gccagcttcg ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa
     5221 gcgatggtcg cttcgcattg agtcagcaaa tcacgtaagt gagtactcgc tttacgcggc
     5281 acaatgccac cgaacagcat cagtgtatga cggactaggc taatggctgc cagcacctgc
     5341 tctttcgccg catcgttgcc gcgtacccac agttcttcat gatactgcca ttgcgctaac
     5401 accagctcga gcgccgcttc cagcccctgt tccacatcgg cttttgccgc aacatgcaaa
     5461 atggtggtcg gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc
     5521 gctttgctca ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc
     5581 agcaccgcgc gcgtgtcgcc gctaagcagc tccagttcca gctcgcagat aggctcagca
     5641 aattcacccg ctttcacttc cccctggtcg agagcgattt caattcggct gccatcgatc
     5701 tctactagcc atttttcgcg ataaaaatcg gtgctaaaca gcgactgcac gcgggaagcg
     5761 agatcggcag gcaattcgcc gttcggccag atttccgtcg gtaactgcgc caggtcgagc
     5821 gtcggttcgc tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt
     5881 cctgcaactt tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata
     5941 tcgtgcctac gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg
     6001 tcatggtgct cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga
     6061 ttaacaataa actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac
     6121 ataagtgacg atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc
     6181 gccaaattgc catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac
     6241 ctgacacaga ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac
     6301 tatcgttcca ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga
     6361 ttaactttac tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt
     6421 tctgacgaac tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc
     6481 acggttaacg ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc
     6541 caggtgaaag acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag
     6601 ccaagcctgc gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa
     6661 ctcaccaata tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg
     6721 cagagcgaca gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg
     6781 attgtcgcgc agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc
     6841 atcatcatgc aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt
     6901 ctggtactgc cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc
     6961 gccttcttcg ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc
     7021 gtgaagattt atctggtcgg tggtgctgtt cgggatgcat tgttaggact atcggtcaaa
     7081 gacagagatt gggtggtggt cggcagtacg ccacaggaga tgctcgacgc gggctaccag
     7141 caggtaggcc gcgattttcc tgtgtttctg catccgcaaa cgcatgaaga gtatgcgctg
     7201 gcacgtaccg aacggaaatc cggttccggt tacaccggtt ttacttgcta taccgcaccg
     7261 gatgtcacgc tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag
     7321 gacgataacg gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg
     7381 ttgcgccatg tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt
     7441 tttgctgcgc gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg
     7501 cgcgagatga cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag
     7561 acggaaagcg ccctcaccac ccgcaatccc caggtgttct tccaggtact gcgcgattgc
     7621 ggcgcacttc gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcacctgcc
     7681 aggtggcatc cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg
     7741 atgctgagtc cgcaggttga tgtccgtttc gcaactttat gccacgatct cggtaaagga
     7801 ctgacgccgc cagaactttg gccgcgtcat catgggcatg gcccggcggg tgttaagtta
     7861 gtggaacaac tatgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg
     7921 gtggctgagt ttcacgatct catccgcacc ttcccaatgc tgaacccaaa aaccatcgtc
     7981 aaattatttg attccatcga tgcctggcgt aaaccgcagc gtatcgagca actggcgctg
     8041 accagcgagg ctgacgtgcg cggcagaacc ggttttgaat cagcggacta cccgcaaggc
     8101 cgttggttgc gcgaaggctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa
     8161 gcgggattta aaggcgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta
     8221 gccagctgga aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttagca
     8281 cgaaccgtcc cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg
     8341 gtgcgaacaa tcacccccac cccggccctc tccctacaga gcgagggaga aaacccggcc
     8401 tgctcccgaa ccttttctca atcaaaagaa cacgacatac accgcagccg ccacaataaa
     8461 gcgataaatg gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat
     8521 cgctatcagc gccaccacaa aagcggtgat aaacccaacg gcaaacatcg ggatatcgcc
     8581 ggttgtcagg aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg
     8641 taccgccagc aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc
     8701 acctgaaatg gtcgccccgg aacgggagaa acccggccac agcgccagac actggaaaca
     8761 gccaatcata aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt
     8821 cggcttcagg cattcggcgg caatcagcaa caaaccgcca acgaccagcg catacatcac
     8881 atttatcggg ttaaacaatg acttaatcgt gtcgtggaat aatagcccca gtaccaccgc
     8941 cggaatcatc cccagcaaaa tgtggatcag cgttaaacga cctttgcttt caccttcgtg
     9001 ctgcaacggg cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac
     9061 tactaccgcc agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc
     9121 ctcaaacccc aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa
     9181 ttctgtcaat ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct
     9241 catcaataaa cccctaaatt attaaaatgt accgcttgtc cgaactactg cagtatgacc
     9301 aggttataac cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc
     9361 acgctcaatg attacgccaa cattcgccgc ccgcgccact gcgcctggct tgctgagttt
     9421 gatacgcacc cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac
     9481 gcgttccacc agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc
     9541 agcgtaactg aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc
     9601 catttcgata tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat
     9661 agtggtgatt accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt
     9721 tggctaaccg gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa
     9781 cacgaatttt caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg
     9841 cgtacctctg cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg
     9901 atccgcgaac cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca
     9961 agggagcagc cgtagcagta ctgattttcg acgttctgaa aggaatgttg cctgtctggg
    10021 gcgcgtatga attaggtgtc agccccttct ggctaggctt aattgccatc gccgcctgtc
    10081 ttggacacat ctggcccgtt ttcttcggtt ttaaaggagg aaaaggcgtt gctaccgctt
    10141 ttggagcaat cgcaccaatt agctgggatc tcaccggagt aatggcggga acctggttac
    10201 tgaccgtgct attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc
    10261 cgttttatgt ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc
    10321 tgatcctgct gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa
    10381 tctggacgaa attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac
    10441 atgacctgat agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat
    10501 tccgcgcaga cacgaatttc atgctgcgat agagcagctc ccggtaaaca gcccagatat
    10561 tggcgctttg tgcatactcc ggcaatactt gcaccagttt gccactctcc agaaacggca
    10621 acacatccca ctcggaacgc agcataatcc ctttgccctc cagcgcccat tgcaacacta
    10681 tctcgccgct attggaggaa agatgcccgc taaccttcac cgattttttc tcctgaccgt
    10741 tccccaactc ccatattccg tgggtcatat cgcgctcttt ggtcaccaga cagtcatgac
    10801 gacttaattc ctgtaatgat tgtggctgcg gatatttttg cagatactca ggtgctgcgc
    10861 ataatattct tttatttttc gttaacagat gcgcaatata ataatcagga atttcgtcat
    10921 taatacgaat atccagatca atattatcct gcactaaatc aatttgccga tcgaacaatt
    10981 caaaatgcac ctgtaactca ggataattgc gcataagttc ggtaatggct ggcgcaatat
    11041 ggctgcgccc aaaaccaaaa ctgcaaccaa tacgaatcat cccctccggg cgcgttttga
    11101 tttgcgtgac gtcatcgacc agtcgctgat attgggtgag gatttccagc gcatgttcgt
    11161 aacaacgttg gccactttca gtcagcgcca cgccgcgagc ggagcggttc agcagcgttg
    11221 ttgccagcgt attttcaagg atttggatcc gcttggtgac aaatgcaggc gtttgcccaa
    11281 gcgtcgccgc tgcagcacta aaactcccgc tgtggacgat ttctaccagt acctgcaagt
    11341 ctttggctaa aggccagcta ttcagcatcg ttaattatcc gcagttgtga taagcgcagt
    11401 gtatttcgca aaacattgcc attccctgct ctggcgcaat attcattctt attttcgcgt
    11461 tgattcacga actgttaatt acatattaac ggtggcaaaa aatataaaac cacattttta
    11521 gtgttagttt gtggcggtga atttttccag acaaatacaa aaactggagt tgccatgatg
    11581 agcgaaagta ataagcaaca ggcagtgaat aagttgacag agattgtcgc taactttacc
    11641 gccatgattt ctacccgaat gcctgatgac gtggtggata aactaaaaca gctaaaggat
    11701 gccgaaacgt cgtcgatggg gaaaattatc taccatacga tgttcgacaa catgcaaaaa
    11761 gcgattgacc tgaatcgtcc tgcctgtcag gacaccgggg agattatgtt cttcgttaaa
    11821 gtcggttccc gcttcccact gcttggcgag ctgcaaagca tactcaaaca agccgtggaa
    11881 gaggcaaccg tcaaagcgcc actacgtcac aatgcggtag aaatttttga cgaagtaaac
    11941 accggcaaaa ataccggtag cggcgtaccg tgggtcacct gggacatcat ccccgacaat
    12001 gacgatgcgg aaatcgaagt ttacatggca ggcggcggct gcacgctacc tggccgctcg
    12061 aaagtgttaa tgccgtcaga aggctacgaa ggcgtggtga aattcgtctt cgaaaatatc
    12121 tccaccctcg ccgtaaacgc ctgtccaccg gtactggtgg gcgtgggcat cgccacctcg
    12181 gtggaaaccg ccgccgtact ctcgcgtaaa gccattttgc gcccgattgg cagccgccat
    12241 cccaatccaa aagcggcaga actggagcta cgcctggaag aaggactcaa ccgtctgggg
    12301 attggtccac aagggctgac cggcaacagt tcagtgatgg gcgcatcgtc gcggcacgct
    12361 gctggttcat gccgatctca cctttgaaaa tctgtctcac acccggagcg cgttatgaaa
    12421 aagatcctga caaccccgat caaagctgaa gatctgcaag atattcgcgt cggcgatgtg
    12481 atctacctga ccggtacgct ggtgacctgc cgcgacgttt gtcaccgccg tttgatcgaa
    12541 ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga ttttccacgc tggccccatc
    12601 gtgcgcaaaa acggcgacaa atgggagatg gtctccgtcg gcccgacaac cagtatgcgt
    12661 atggaaagtt ttgaacgtga atttattgag cagaccggcg taaaactggt ggttggcaaa
    12721 ggtggtatgg ggccgctgac cgaagaaggc tgccagaaat tcaaggcgtt acatgtgatt
    12781 ttcccagcag gctgcgcggt gctggcggca acccaggtag aagagattga agaagtgcac
    12841 tggacagaac tcgggatgcc ggagtcactg tgggtctgcc gggtcaaaga gttcggcccg
    12901 ctgattgtct ctattgatac ccacggcaac aacctgatag ccgaaaacaa aaagctgttc
    12961 gccgaacgcc gcgatcccat cgtggaagag atctgcgagc acgtccatta catcaaataa
    13021 ccctcccgga gaggctcacc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    13081 ctgaatggtg gcgatatctt gcgccgctgg cggtcatcgc cattattgct ctacttccgg
    13141 ttcccgcagg gctggagaac catacctggc tctactttgc cgtttttact ggcgtgatcg
    13201 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atctccatca
    13261 tcgccatact ctctccctgg ctgctgttca gccccgaaca gctcgcccag ccaggcttta
    13321 agttcaccgc caaatccctc tcctgggcag tttctggttt ttctaattcg gttatctggc
    13381 tcattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    13441 ttgcgctgat tctggtgaaa aagatggggc atcgcacgct gtttctcggc tatgcggtga
    13501 tgttctccga gctgatcctg gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggta
    13561 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    13621 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    13681 gcgccatttt cctgacggcg atggcaccta acttgctgtt aattggactg atgaaaagcg
    13741 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagcattt
    13801 tactggttct gctggttccc tggctggctt acgtgctgta cccgccggta ctgaagtctg
    13861 gtgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    13921 gtgaaaaacg gatgctgggg ctgatggtag gcgcgctggt gctgtggatt ttcggcggtg
    13981 attatatcga tgccgcgatg gtcggttaca gcgtggtggc gctgatgctg cttctgcgca
    14041 ttatcagttg ggacgacatt gtcagtaata aagcggcatg gaacgttttc ttctggctgg
    14101 cctcgcttat caccctcgcg accggactca acaataccgg ttttattagc tggtttggca
    14161 aactgttagc aggcagctta agtggttatt cgccgacgat ggtgatggtg acgttgattg
    14221 tggtgtttta tctactgcgc tactttttcg ccagcgccac ggcgtatacc tgcgccctcg
    14281 caccgatgat gatcgccgcc gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    14341 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    14401 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    14461 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    14521 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    14581 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    14641 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaat
    14701 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    14761 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    14821 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    14881 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    14941 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    15001 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    15061 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    15121 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    15181 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    15241 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    15301 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    15361 acagcatcaa tgtcttttgc cgttaaacca gactccttta gcgccgcctg gatcaacggt
    15421 acggttttac gcacatgatc gcgggaggcc agttcaggca cgacgccgcc gtagtcagcg
    15481 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    15541 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    15601 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    15661 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaaggccgcg
    15721 gtgctttaca aagcagcagc aattgcagta aaattccgca ccattttgaa ataagctggc
    15781 gttgatgcca gcggcaaacc gaattaatca aaggtgagag gcacatgccg gtaattaaag
    15841 tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt caagcgttcc tgcgaaaaag
    15901 caggtgttct ggcggaagtt cgtcgtcgtg agttctatga aaaaccgact accgaacgta
    15961 agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa actggctcgc gaaaacgcac
    16021 gccgcactcg tctgtactaa ttccccgaga gcgttgctct ccgatcagac cgagttgtag
    16081 ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt tcgtttatga attgctaaaa
    16141 atcggggcct atggctggac gaatcccacg cgtattcatt aatgatctgc tggcacgcac
    16201 tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa aagcagggca agaatttcca
    16261 cgcgtgttgt ccattccaca acgagaaaac cccgtcattc accgttaacg gtgagaaaca
    16321 gttttaccac tgctttggat gtggcgcgca cggcaacgcg atcgacttcc tgatgaacta
    16381 cgacaagctc gagttcgtcg aaacggtcga agagctggca gcaatgcaca atcttgaagt
    16441 gccatttgaa gcaggcagcg gccccagcca gatcgagcgc catcagaggc aaacgcttta
    16501 tcagttgatg gacggtctga atacgtttta ccaacaatct ttacaacaac ctgttgccac
    16561 gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac gaggttatcg ctcgctttgc
    16621 gattggtttt gcgccccccg gctgggacaa cgtcctgaag cggtttggcg gcaatccaga
    16681 aaatcgccag tcattgattg atgcggggat gttggtcact aacgatcagg gacgcagtta
    16741 cgatcgtttc cgcgaacggg tgatgttccc cattcgcgat aaacgcggtc gggtgattgg
    16801 ttttggcggg cgcgtgctgg gcaacgatac ccccaaatac ctgaactcgc cggaaacaga
    16861 cattttccat aaaggccgcc agctttacgg tctttatgaa gcgcagcagg ataacgctga
    16921 acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg cgcaatacgg
    16981 cattaattac gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca tacaactgtt
    17041 gttccgcgcg accaacaatg tcatttgctg ttatgacggc gaccgtgcag gccgcgatgc
    17101 cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca gacggccgtc agctacgctt
    17161 tatgtttttg ccggatggcg aagaccctga cacgctggta cgaaaagaag gtaaagaagc
    17221 gtttgaagcg cggatggagc aggcgatgcc actctccgca tttctgttta acagtctgat
    17281 gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc tggcactgcc
    17341 attgatatcg caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg aattaggcaa
    17401 caaattaggc atacttgatg acagccagct tgaacgatta atgccaaaag cggcagagag
    17461 cggcgtttct cgccctgttc cgcagctaaa acgcacgacc atgcgtatac ttatagggtt
    17521 gctggtgcaa aatccagaat tagcgacgtt ggtcccgccg cttgagaatc tggatgaaaa
    17581 taagctccct ggacttggct tattcagaga actggtcaac acttgtctct cccagccagg
    17641 tctgaccacc gggcaacttt tagagcacta tcgtggtaca aataatgctg ccacccttga
    17701 aaaactgtcg atgtgggacg atatagcaga taagaatatt gctgagcaaa ccttcaccga
    17761 ctcactcaac catatgtttg attcgctgct tgaactgcgc caggaagagt taatcgctcg
    17821 tgagcgcacg catggtttaa gcaacgaaga acgcctggag ctctggacat taaaccagga
    17881 gctggcgaaa aagtgattta acggcttaag tgccgaagag cgatcgggaa gcccccgaca
    17941 gccgcactga gaggcagcgg caaatatata agtacgccct cgtaattatc gttggcggta
    18001 aacaaccgtt ggatttcagc gttaacggct gaaggacatc gggtcaatcg cccaacacca
    18061 acctcatgaa ataagtgtgg ataccgtctt atggagcaaa acccgcagtc acagctgaaa
    18121 cttcttgtca cccgtggtaa ggagcaaggc tatctgacct atgccgaggt caatgaccat
    18181 ctgccggaag atatcgtcga ttcagatcag atcgaagaca tcatccaaat gatcaacgac
    18241 atgggcattc aggtgatgga agaagcaccg gatgccgatg atctgatgct ggctgaaaac
    18301 accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc tttccagcgt ggaatctgaa
    18361 atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg aaatgggcac cgttgaactg
    18421 ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg aagacgggat caaccaggtt
    18481 caatgctccg ttgctgaata tccggaagcg atcacctatc tgctggaaca gtacgatcgt
    18541 gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg gctttgttga cccgaacgca
    18601 gaagaagatc tggcacctac cgccactcac gtcggttctg agctttccca ggaagatctg
    18661 gacgatgacg aagatgaaga cgaagaagat ggcgatgacg acagcgccga tgatgacaac
    18721 agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac tacgcgctca gtacgttgta
    18781 acgcgtgaca ccatcaaagc gaaaggtcgc agtcacgctg ccgctcagga agagatcctg
    18841 aaactgtctg aagtattcaa acagttccgc ctggtgccga agcagtttga ctacctggtc
    18901 aacagcatgc gcgtcatgat ggaccgcgtt cgtacgcaag aacgtctgat catgaagctc
    18961 tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta ccctgtttac cggcaacgaa
    19021 accagcgata cctggttcaa cgcggcaatt gcgatgaaca agccgtggtc ggaaaaactg
    19081 cacgatgtct ctgaagaagt gcaccgcgcc ctgcaaaaac tgcagcagat tgaagaagaa
    19141 accggcctga ccatcgagca ggttaaagat atcaaccgtc gtatgtccat cggtgaagcg
    19201 aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact tacgtctggt tatttctatc
    19261 gccaagaaat acaccaaccg tggcttgcag ttccttgacc tgattcagga aggtaacatc
    19321 ggcctgatga aagcggttga taaattcgaa taccgccgtg gttacaagtt ctccacctac
    19381 gcaacctggt ggatccgtca ggcgatcacc cgttctatcg cggatcaggc gcgcaccatc
    19441 cgtattccgg tgcatatgat tgagactatc aacaaactca accgtatttc tcgccagatg
    19501 ctgcaagaga tgggccgtga accgacgccg gaagaactgg ctgaacgtat gctgatgccg
    19561 gaagacaaga tccgcaaagt gctgaagatc gccaaagagc caatctccat ggaaacgccg
    19621 atcggtgatg atgaagattc gcatctgggg gatttcatcg aggataccac cctcgagctg
    19681 ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa cgcacgacgt gctggctggc
    19741 ctgaccgcgc gtgaagcaaa agttctgcgt atgcgtttcg gtatcgatat gaacaccgac
    19801 cacacgctgg aagaagtggg taaacagttc gacgttaccc gcgaacgtat ccgtcagatc
    19861 gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt ctgaagtgtt gcgtagcttc
    19921 ctggacgatt aatcggtagg ccggatcagg cgttacgccg cacccggcac tagtctctct
    19981 gcacaaacgc caccttt
//
LOCUS       DAJTRH010000074        19336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTRH010000074 DAJTRH010000000
VERSION     DAJTRH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="OO339_002800"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="OO339_002800"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5927368.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="OO339_002801"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="OO339_002801"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5927369.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO339_002802"
     CDS             2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO339_002802"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5927370.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="OO339_002803"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="OO339_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5927371.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="OO339_002804"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="OO339_002804"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5927372.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="OO339_002805"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="OO339_002805"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5927373.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="OO339_002806"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="OO339_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5927374.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="OO339_002807"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="OO339_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5927375.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="OO339_002808"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="OO339_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5927376.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="OO339_002809"
     CDS             11296..11511
                     /locus_tag="OO339_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5927377.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO339_002810"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO339_002810"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5927378.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="OO339_002811"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="OO339_002811"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5927379.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="OO339_002812"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="OO339_002812"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5927380.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="OO339_002813"
     CDS             complement(16247..16786)
                     /locus_tag="OO339_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5927381.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="OO339_002814"
     CDS             complement(16802..17320)
                     /locus_tag="OO339_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5927382.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="OO339_002815"
     CDS             complement(17631..17822)
                     /locus_tag="OO339_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5927383.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="OO339_002816"
     CDS             17840..17992
                     /locus_tag="OO339_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927384.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18174..19313
                     /gene="pdeF"
                     /locus_tag="OO339_002817"
                     /pseudo
     CDS             18174..19313
                     /gene="pdeF"
                     /locus_tag="OO339_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
BASE COUNT         4554 a         4705 c         5259 g         4818 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa
    18001 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
    18061 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
    18121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
    18181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
    18241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
    18301 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
    18361 cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
    18421 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
    18481 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
    18541 agcgtatatt ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg
    18601 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
    18661 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
    18721 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
    18781 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgagctctgg ctactggcat
    18841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
    18901 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt
    18961 ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
    19021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
    19081 tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag
    19141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
    19201 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
    19261 gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaatat gaggtgtact
    19321 ggcaatagcg gacact
//
LOCUS       DAJTRH010000075        19113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTRH010000075 DAJTRH010000000
VERSION     DAJTRH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..270)
                     /locus_tag="OO339_002818"
     CDS             complement(<1..270)
                     /locus_tag="OO339_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5927385.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQ"
     gene            complement(301..1167)
                     /gene="ydiB"
                     /locus_tag="OO339_002819"
     CDS             complement(301..1167)
                     /gene="ydiB"
                     /locus_tag="OO339_002819"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5927386.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1179..2451)
                     /locus_tag="OO339_002820"
                     /pseudo
     CDS             complement(1179..2451)
                     /locus_tag="OO339_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2634..3839)
                     /locus_tag="OO339_002821"
     CDS             complement(2634..3839)
                     /locus_tag="OO339_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5927387.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(3939..4295)
                     /locus_tag="OO339_002822"
     CDS             complement(3939..4295)
                     /locus_tag="OO339_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5927388.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4431..4538)
                     /gene="rprA"
                     /locus_tag="OO339_002823"
     ncRNA           complement(4431..4538)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO339_002823"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(4724..5836)
                     /gene="ydiK"
                     /locus_tag="OO339_002824"
     CDS             complement(4724..5836)
                     /gene="ydiK"
                     /locus_tag="OO339_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5927389.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6225..9281
                     /locus_tag="OO339_002825"
     CDS             6225..9281
                     /locus_tag="OO339_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5927390.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9278..9688
                     /gene="menI"
                     /locus_tag="OO339_002826"
     CDS             9278..9688
                     /gene="menI"
                     /locus_tag="OO339_002826"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5927391.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            9788..9976
                     /locus_tag="OO339_002827"
     CDS             9788..9976
                     /locus_tag="OO339_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5927392.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10524..10892
                     /gene="sufA"
                     /locus_tag="OO339_002828"
     CDS             10524..10892
                     /gene="sufA"
                     /locus_tag="OO339_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5927393.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            10901..12388
                     /gene="sufB"
                     /locus_tag="OO339_002829"
     CDS             10901..12388
                     /gene="sufB"
                     /locus_tag="OO339_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5927394.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12398..13144
                     /gene="sufC"
                     /locus_tag="OO339_002830"
     CDS             12398..13144
                     /gene="sufC"
                     /locus_tag="OO339_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5927395.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13119..14390
                     /gene="sufD"
                     /locus_tag="OO339_002831"
     CDS             13119..14390
                     /gene="sufD"
                     /locus_tag="OO339_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5927396.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14387..15607
                     /gene="sufS"
                     /locus_tag="OO339_002832"
     CDS             14387..15607
                     /gene="sufS"
                     /locus_tag="OO339_002832"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5927397.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            15620..16036
                     /gene="sufE"
                     /locus_tag="OO339_002833"
     CDS             15620..16036
                     /gene="sufE"
                     /locus_tag="OO339_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5927398.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16185..17189
                     /gene="ldtE"
                     /locus_tag="OO339_002834"
     CDS             16185..17189
                     /gene="ldtE"
                     /locus_tag="OO339_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5927399.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17252..17488)
                     /gene="lpp"
                     /locus_tag="OO339_002835"
     CDS             complement(17252..17488)
                     /gene="lpp"
                     /locus_tag="OO339_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5927400.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(17799..>19113)
                     /gene="pykF"
                     /locus_tag="OO339_002836"
     CDS             complement(17799..>19113)
                     /gene="pykF"
                     /locus_tag="OO339_002836"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5927401.1"
                     /translation="NFSHGDYAEHGQRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKL
                     EGGNDVSLKAGQTFTFTTDKSVIGNSEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVT
                     AIEGNKVICKVLNNGDLGENKGVNLPGVSIALPALAEKDKQDLIFGCEKGVDFVAASF
                     IRKRSDVIEIREHLKAHGGENIHIISKIENQEGLNNFDEILEASDGIMVARGDLGVEI
                     PVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAV
                     MLSGESAKGKYPLEAVSIMATICERTDRVMNSRLEFNNDNRKLRITEAVCRGAVETAE
                     KLDAPLIVVATQGGKSARAVRKYFPDATILALTTNEKTAHQLVLSKGVVPQLVKEITS
                     TDDFYRLGKELALQSGLAHKGDVVVMVSGALVPSGTTNTASVHVL"
BASE COUNT         5002 a         4621 c         4896 g         4594 t
ORIGIN      
        1 ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt ctggcatggt
       61 ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt cggcaaagtg
      121 gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag cttcggattt
      181 cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc ccgcaccaat
      241 gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc ggtcagcctg
      301 tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt tgccagtcca
      361 taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa tcgttttgca
      421 accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca cgcattcagt
      481 gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat tctcaagggg
      541 tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg cttcagcaaa
      601 ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt cgttaacccg
      661 ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga gtttaatttc
      721 ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc cggcccctaa
      781 cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc gaatatggcc
      841 cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg tgttgatggc
      901 acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca gttgtttgtt
      961 cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa ttgctccggg
     1021 aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg ctttttctaa
     1081 ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca tcaatccaat
     1141 caattcgtat tttgcggtaa catccataat taactccttt aacctctatg cttaattgtg
     1201 tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg aataataaaa
     1261 acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag cgcgaaagta
     1321 aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt aatcagcgga
     1381 ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt ggctttgctt
     1441 ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc agctgctgag
     1501 aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag gtagataatg
     1561 gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg ccggaccatt
     1621 tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact gtaataagag
     1681 atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata tttgggcatc
     1741 cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac cgatgaaaca
     1801 ccttccagcc agactaaagg ttttgctgtt catttgcggt aattcattag ctacgctggc
     1861 gtccaccaac tggctgggga atttgctttt caacagcatc agcgtgatca gtacaaacag
     1921 aatacccgga ataatcagcc catagccgta ccagatatta ttgagcaaca tatagctcac
     1981 cagcattggg tagaacattt gcccaaatga caccatcgct ttaaccagta tgaccgccga
     2041 accagaggct ttcggaaagc attccatgag cgcggggtag ccacccgtat ccagcgctga
     2101 gttagcgata cctacgcaca ctgccagacc gtaggcgaga gttaaattcg ggcaagcggg
     2161 aataccaaag aagaatagca gatacattat tactgccatt aatatcaccg cccgacgacc
     2221 aaacttatcg gagatcacac cgaagaataa aatactgatc aatcgcccca aaccgatacc
     2281 ggaaattaaa taggcaatgc ccgcgttgtc agtggaaaac ttttctgaca gagatgtcat
     2341 attttgggca agcgtaataa cactaatgcc gtgcaggaaa tagctgaagt aaatacaaag
     2401 aacagccagg ataaatggcg tgctgaaaga taatttcgtt accgtacgta ttgttctccg
     2461 acggtttaca taattactat actgaagatg attttccctg ggtacatggc ataaagttta
     2521 tgtttccagg aatattgcat gtcatagttt gtttttgatc gcgattttcc ttagttagcg
     2581 cagaaagagt tatttatccg taagatcttg catgacctga aaagaatatt gcattaccca
     2641 cccggagcga cattttcctt tagcgagtga tgccgcgttt ttttgcggct gcgtaagccg
     2701 ataaacagtg ccagtaaaaa accgatggca gtatcgaacc acataatatc ggcaatactt
     2761 ctttgggaca gatgagccgt aatcaacgga atagtaaagg tcgcaatact gcccgtactg
     2821 taatagatcc ctgtagcttt acctttagcg taagggaaac gttcagccat taacgtcagg
     2881 ccaatttgca caacacctcc agcagaggta aaaccaatga caaaagcaaa tattatcacc
     2941 acataaaatg tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac
     3001 agcatcagta atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca
     3061 caaagcagcg aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg
     3121 cctgcaacaa attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt
     3181 gagatatagc catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta
     3241 gagctggtgg ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa
     3301 cggtataaaa acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg
     3361 aaccacagtt cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg
     3421 gaaacaaatg ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc
     3481 aaactgggat aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa
     3541 acataagcga taatgatgtt attggtctgc aggatgtcaa aaaagaaggc catatagcag
     3601 cacatcccga gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa
     3661 agcaagacac ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca
     3721 ttagtctgcc aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata
     3781 ccatgcacca ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata
     3841 ggttctactt agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat
     3901 tattgttgta acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg
     3961 tgtgcatata attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca
     4021 gggtagactt gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg
     4081 cggttattta ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc
     4141 aacagttgct cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag
     4201 ctttcactgt caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa
     4261 atatggcgga gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac
     4321 aataaaatat aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg
     4381 tgcaacatta ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa
     4441 gcccatcgtg ggagatggga aaagactaca cacagcaatt cgttgtttca ctcaggggat
     4501 ttccatgctt ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga
     4561 ctttttgcgt cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg
     4621 tactaccagc tgaatggcgg taagcaggaa aatatgggga agagcaagca aatatcaatt
     4681 agcggtgatt aacaatcagg cggcagagcc gcctgatgag aaattactta ttcggtttct
     4741 cgatttcgcc aagttcttcg agaatttctt ccggttggtc cgtcggtggc gggacttctt
     4801 ccacccacgc ggcaaacaga cgccaggaaa cggctaacag aaccggacca ataaacagac
     4861 cgatcatccc gaaagcaatc aaaccaccaa taacgccaga gaggatcagg atcagcggta
     4921 aatcggcacc catgcgaatt aacattgggc ggatgacgtt atccagcgtg ccaaccacac
     4981 cgctccacac taacaatacc gttccccagg tggtatcgcc ggtccagtag agccagataa
     5041 tcgccggaat cagtaccggc aacgggccaa gctggacaag gcaggagagg atcattaaca
     5101 ccgttagcaa cgttgcataa ggtacgccgg atacggcgag gccgataccg ccaagcactg
     5161 cctgtactaa cgccgtcacc accacaccca gcgccaccgc gcggatagcc tgtgccgcca
     5221 gcagcacggc ggcatctccg cgaacgcctg ccagacgggt tgcaaaatgg cgaatgcctt
     5281 gtgccacctg ttcaccgcgc cagtacagca gggcactgaa gagaagcatc agcgcacaat
     5341 gcaccataaa gcgcccgata tgcgccgcct gcccaacgaa ccaggtggtg gtggtgccaa
     5401 tataagggcg gactttcgcc atgatcgccg taccccccat atccagcaag ttgtgccagc
     5461 ctgcatacag cttcgcgcca atcaccggaa tggtattaag ccacgccaga tcgggtaacg
     5521 tcatgtcgcc gctggaaatg gctttaatta gcgggccgct gccgtcgacg atactgttaa
     5581 ccagcaaagc aatagggatg ataaacacca tcactaataa cagcgtcatc accagaacgg
     5641 cgagagagcg gcggccaaac atgatctttt gcaaacgtaa caataccggc caggtggcga
     5701 taaccaccgt accggcccat gcaaagccga gaataaaggg ttgaacaatc cacagacatg
     5761 ccacaatcat gatggctaaa aacagcaccg aaagcagaat ttgtgcgaca tccctgggct
     5821 gacgaacatt taccataact acttttcacc ttttttgtgc gtcaaatcgt cgacgcgacg
     5881 tgaacacgcg aaactcacct gcataatgat gagcaatttc agcggttttt aacaggccga
     5941 ttctgccctt aattctgatg ggcgtgttag aaaaaaatgt ggtacaacaa ctcaggcaac
     6001 acgcaaacga tttactcgtc gtatttcaag ttgcatgtgc tgcggctacg ttcgttcacc
     6061 ccagtcactt actattgtaa gctcctggag ttcactcgct tgccgccttc ctgcaactcg
     6121 aaatccatag agtaaataaa catcaccttt cagtgcgcta ttgcagccgc ctgaaagaat
     6181 agtgtatcga cctacatcac agaccgcagg aaagggtcaa tataatgatt ccacagattt
     6241 ccctggcacc cggcgtcgtt caactggtgc ttaatttttt gcaagagctg gagcaacaag
     6301 gttttaccgg cgatgcggcg acaagttatg ccgatcgtct gacaatgtcg accgacaaca
     6361 gtatttacca acttctcccc gatgcggtgg tatttccgcg ttcaaccgca gatgtggcgc
     6421 tgatcgcccg tcttgccgcg caggaacgct attcatcgct gatctttacc ccccgcggcg
     6481 gcggcaccgg cactaacggt caggcgctca accaggggat tattgttgat atgtcccgcc
     6541 atatgaaccg catcatcgaa attaaccctg aagagggctg ggtgcgcgtt gaggccgggg
     6601 tgataaaaga ccaactcaat cagtacctga aaccgttcgg ctactttttt gcgccggaac
     6661 tttcgaccag caaccgggca acgctcggtg ggatgatcaa taccgatgca tccggtcagg
     6721 gatcgctggt ctatggcaaa acgtcagatc acgtacttgg cgtacgcgcg gtgttgttgg
     6781 ggggcgatat tctcgatacg caacctttac ccgtcgagct ggcggaaacg ctgggtaaat
     6841 ccaataccac aatcgggcga atttataaca cggtttatca acgttgccgt cagcaacgcc
     6901 agttaatcat cgacaacttc cccaaactta accgctttct taccggttac gatctgcgtc
     6961 atgtctttaa cgatgagatg accgagttcg acctgacgcg cattctgacg ggttcagaag
     7021 ggacgctggc ctttattacc gaagcgcggc tggatattac gcgcttgcct aaagtgcgcc
     7081 gtctggtgaa tgtcaaatat gactcttttg actctgcgct gcgtaacgcg ccgtttatgg
     7141 ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa agtgctgaat ctggcgcggg
     7201 aagatattgt ctggcattcc gtcagcgagt tgattaccga tatgcctgac aaagagatgc
     7261 tcggactgaa cattgtggaa tttgctggtg atgatgaggc gctgattgac gagcgagtta
     7321 atgcactctg tgtgcggctt gatgagctga ttgccagcca acaggcaggt gtgattggct
     7381 ggcaggtgtg ccgcgagctg gcgggggttg aacgtatcta tgcgatgcgc aaaaaagccg
     7441 ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct gaggatacct
     7501 gcgtaccgcc ggaacatctg gccgattata ttgctgaatt tcgcgcgctg ctcgacagcc
     7561 acggcttaag ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac gtccgtccgg
     7621 cactggatat gtgcgatccg caacaagaga ttttgatgaa gcaaatctct gatgacgtgg
     7681 tggcgctgac tgcgaaatac ggtggtttgt tgtggggcga gcacggcaaa ggttttcgcg
     7741 ctgaatacag cccggcgttt ttcggtgagg aactttttgc agaactgcgc aaagtgaaag
     7801 cggcatttga cccgcataac cgactcaacc cagggaagat ttgcccgcca gaaggtctcg
     7861 atgcgccgat gatgaaagtg gacgcggtga agcgcggtac attcgatcgg cagatcccca
     7921 ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt ttatgcttca
     7981 actttgatgc ccgtagtccg atgtgtccgt cgatgaagat cacccagaac cggattcatt
     8041 caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg cttgttggcg gatcgcggcg
     8101 ttgacccact caaactggaa caagaactgc ctgaatccgg cgtcagtttg cggacgttaa
     8161 ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga atatgacttc tcacacgaag
     8221 tcaaagaggc gatgtcgggc tgtctggcct gtaaagcttg ttcgacccag tgccccatca
     8281 aaattgatgt gccggagttt cgctctcgtt ttctgcagct ctatcacacc cgttatttac
     8341 gcccgcttcg cgaccacctc gttgctacgg tcgagagcta cgcaccgctg atggcacgcg
     8401 cgccgaaaac ctttaacttc ttcattaacc agccgctggt gcgcaaactc tcggaaaaac
     8461 atatcggcat ggttgatttg ccactgctgt cggtcccctc gctacaacaa caaatggtgg
     8521 ggcatcgctc ggcaaacatg acgctggaac agcttgaagc tctcaatgca gagcagaaag
     8581 cgcgcacggt gttggtggtg caggacccct ttaccagcta ttacgatgcg caagtggtgg
     8641 cggattttgt ccgtctggtt gaaaaattag gtttccagcc tgtgttactg ccattttcgc
     8701 caaatggcaa agcccagcat atcaaaggct ttcttaatcg ttttgcgaag acggcgaaaa
     8761 agacggcgga tttcctcaac cgtatggcta agcttgggat gccaatggtg ggcgtcgatc
     8821 cggcgctggt actttgttat cgcgatgaat ataaactggc gctgggcgag gaacgtggcg
     8881 agtttaacgt cttacttgcg aatgaatggc tggcaagcgc acttgagtca catccagtgg
     8941 ctacagtcag cggtgaatca tggtatttct ttggtcactg taccgaggtt accgctttgc
     9001 cgggcgcgcc agcacaatgg gccgcgatat ttgcccgttt tggcgcgaaa ctggaaaatg
     9061 tcagcgtggg ttgctgcggc atggcaggga cttacggaca tgaagcgaaa aaccatgaaa
     9121 attcgctcgg gatctatgag ttatcctggc atcaggctat gcagcgactg ccgcgtaacc
     9181 gctgtctggc gaccggatat tcctgccgta gccaggtaaa acgggttgaa ggcacggggg
     9241 tacgccatcc tgtgcaggct ttactggaga ttattaaatg atatggaaac ggaaaatcac
     9301 cctggaagca ctgaatgcta tgggtgaagg aaacatggtg gggttcctgg atattcgctt
     9361 tgaacatatt ggtgatgaca cccttgaagc gacaatgcca gtagactcgc ggacaaagca
     9421 gcctttcggg ttgctgcatg gaggagcatc cgtggtactg gccgaaagta tcggttccgt
     9481 tgccggttat ttatgtaccg aaggtgagca aaaagtggtt ggtctggaaa tcaatgctaa
     9541 tcacgtccgc tcggcacgag aagggcgggt gcgcggcata tgtaaaccgt tgcatctcgg
     9601 ttcgcgtcac caggtctggc agattgaaat cttcgatgag aaagggcgtt tgtgctgttc
     9661 gtcacgattg acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga cgcatattgc
     9721 ttttggtcat tatctgcaca gtaaacggtg gtatactgtg caggtcttgt ctaaaagcga
     9781 ggatgctatg tctactcaac ttgatcccac ccagctggcc attgaatttt tacgccgtga
     9841 ccagagcaat ctttcacctg cccaatatct caaacgtctg aaacaactag aacttgaatt
     9901 tgctgacctg cttactctct cttctgctga attaaaagaa gaaatctact tcgcctggcg
     9961 tctgggcgtg cattgatcct gacttaatgc cgcaagttcg ccaaatcgac ctggattaac
    10021 ctccgtccag gttcgatgac cttcttaata ctgcttatct accgcctgat attgttcaaa
    10081 aataatacta atcgcagttt gtgttaaaac ggcgggttag ctttatgaga ttattcttat
    10141 cgccccttca agagctaagc cactgagagt gccggagata agcgccggat ggggtagaaa
    10201 cccttaagcc tgtgtcgcac agacttaagg gttttcttat ttctgaacgc tgcacttttt
    10261 tacctatctc ttaaatagtc attttcaata caactttttc tttttcatca cagccttaat
    10321 gctccttaga taataaccat tatctaacaa tgagatacct aattcttagc gtgcctgtta
    10381 acccacacat caggggctat gcttattaaa agcaggcaaa aagaatgttt tacggtaaag
    10441 cccctgcgtt tgctgggttg aactgataat cattatcact aacatgctgt tatacgctga
    10501 aagcgatgaa gtgaggtaaa tagatggaca tgcattcagg aacctttaac ccacaggatt
    10561 tcgcctggca aggcttaacg ctgacacccg cagcggcgat acacatccgc gagctggtgg
    10621 caaagcagcc aggtatggtc ggcgtgcgct taggtgtgaa gcaaacgggc tgcgcgggct
    10681 ttggctatgt gctcgacagt gttagcgagc cggacaaaga cgatctgctg tttgaacacg
    10741 acggcgcgaa gctgtttgtc ccgctgcaat cgatgccgtt tattgatggc acggaagtcg
    10801 atttcgttcg tgaaggactt aatcagatat tcaaatttca caaccctaaa gcccagaatg
    10861 aatgtggctg tggcgaaagc tttagggtat aggcggtact atgtctcgta atactgaagc
    10921 aactgacgat gtcaaaacct ggaccggcgg cccgctgaac tataaagaag gattcttcac
    10981 ccagttagcc accgatgagc tggcaaaggg gataaacgaa gaggtggtgc gcgcaatttc
    11041 ggcgaagcgt aatgagccgg agtggatgct ggagtttcgt ctaaacgcct atcgcgcatg
    11101 gctggagatg gaagaaccgc actggttgaa agcgcactac gacaagctga attatcagga
    11161 ttacagctac tactcagcac catcgtgcgg taattgtgac gacacttgcg cgtctgaacc
    11221 tggcgcggtg cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg aggcggcgtt
    11281 tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg gcggtggatg ccattttcga
    11341 ctctgtttcg gttgccacca cttatcgcga aaaactggcg gagcagggaa ttattttctg
    11401 ttcctttggt gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc tcggcaccgt
    11461 ggtgccgggg aatgacaact tctttgcagc acttaatgcg gcggtagcct ctgacggtac
    11521 gtttatttat gtgcctaaag gcgtgcgctg cccgatggaa ctttccacct attttcgcat
    11581 taacgcggaa aaaaccgggc agtttgagcg cacaattctg gtggccgacg aagacagcta
    11641 cgtcagctac attgaaggct gttccgctcc ggtgcgtgac agctatcagt tgcacgcggc
    11701 agtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg tacaaaactg
    11761 gttccctggc gataacaaca ccggcggtat tctcaacttc gtcaccaagc gtgctttgtg
    11821 cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa accgggtcag cgattacgtg
    11881 gaaatatccc agctgcattt tgcgcggcga taactccatt ggtgagtttt actcagtggc
    11941 gctgaccagc ggtcatcagc aagcggatac cggcaccaag atgatccaca tcggtaaaaa
    12001 caccaaatcg accattatct cgaaagggat ctctgccgga catagtcaga acagttatcg
    12061 cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc aatttcactc agtgcgactc
    12121 aatgctgatt ggcgctaatt gtggggcgca taccttcccg tatgtcgaat gtcgcaataa
    12181 cagcgcacaa ctggagcacg aggcaacgac atcacgtatt ggtgaagatc aactgtttta
    12241 ctgcctgcaa cgcgggatca gcgaagaaga cgccatctcg atgattgtta acggtttctg
    12301 caaagacgtg ttctcagaac tgccgctgga atttgccgtt gaagcacaaa aactcctcgc
    12361 catcagtctt gaacacagcg tcggataagg aataaacatg ttaagtatta aagatttaca
    12421 cgtcagcgtg gaagataaag ctatcctgcg tggattaagc ctcgacgttc gtcccggcga
    12481 agttcacgcc attatggggc caaacggttc gggcaaaagt accttatcgg caacgcttgc
    12541 cgggcgcgaa gattatgaag tgacgggcgg cacggttgag ttcaaaggca aagatttgct
    12601 tgcactgtcg ccggaagatc gtgcaggcga aggcatcttt atggccttcc agtatccggt
    12661 ggagattcca ggcgtcagta accagttttt cctgcaaacg gcacttaatg cagtgcgcag
    12721 ctatcgcggc caggaaacgc tcgaccgctt tgattttcag gatttgatgg aagagaaaat
    12781 cgctctcctg aagatgccgg aagatttatt aacccgttcg gtaaacgttg gtttttccgg
    12841 cggcgagaaa aagcgcaacg atattttgca aatggcggtg ctggaaccgg agttatgcat
    12901 tcttgatgag tcggactccg ggctggatat tgacgcatta aaagtggtcg ccgatggcgt
    12961 gaactcgctg cgtgatggca agcgctcatt catcattgtt acgcactacc aacgcattct
    13021 cgactacatc aagcctgatt acgttcatgt gctatatcag ggacgaattg tgaaatccgg
    13081 cgatttcacg ttggtcaaac aactggagga gcagggttat ggctggctta ccgaacagca
    13141 gtaatgcgct gcaacagtgg catcacttgt ttgaagctga agggacaaaa cgctcaccgc
    13201 aagcacagca gcatttacaa caattgctgc gtaccggact gccgacacgt aaacatgaaa
    13261 actggaaata tacgccgctg gaagggctga ccaatagtca gtttgtcagc attgcgggag
    13321 atatatcccc acagcagcgt gatgccttag cgttaacgtt agacgccgtg cggctggtgt
    13381 ttgtcgatgg acgttacgtg cccgcgctga gcgatgcaat tgaaggcagc ggatatgaag
    13441 tgagcattaa cgacgaccgt caggggttac ccgacgctat tcaggcggaa gtgtttctgc
    13501 atttgacgga aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc ggtcaacggc
    13561 cggcaaagcc attgctgtta atgcatatca cccagggcgt ggcaggtgaa gaggtgaaca
    13621 ctgcccatta ccggcatcat ctggatctgg cggaaggtgc cgaagcaacg gtgatcgaac
    13681 attttgtcag ccttaatgat gctcgtcatt ttaccggggc acggttcact atcaatgtcg
    13741 cagcgaacgc ccacttgcag catatcaagc tggcgtttga aaacccggtc agtcaccact
    13801 ttgcccataa cgatttgttg ctggctgacg atgccaccgc atttagccat agtttcctgc
    13861 tgggtggcgc agtgttacga cacaacacca gtacgcaact caatggcgaa aacagcacgc
    13921 tgcggatcaa tagcctggcg atgccggtga aaaacgaggt gtgtgatacc cgtacctggc
    13981 tggaacacaa taaaggtttt tgtaacagcc gacagttgca caaaactatc gtcagcgaca
    14041 aaggccgcgc ggtatttaac ggtttgatca acgtcgcgca gcacgccatc aaaacggatg
    14101 gtcagatgac caacaataat ctgctgatgg gcaaactggc ggaagtggat acaaaaccgc
    14161 agctggaaat ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg gggcgtattg
    14221 atgatgaaca gatgttctat ctgcgctcgc gcgggatcaa tcagcaggat gcccagcaga
    14281 tgatcattta cgcctttgct gccgaactga cggaagcact gcgtgatgag gggcttaaac
    14341 agcaggtgct ggcccgaatc ggtcaacggc tgccaggagg tgcaagatga ctttttccgt
    14401 cgacaaagtg cgggccgact ttccggtgct ttcgcgtgag gtaaacggtt tgccgctggc
    14461 ttatctcgac agcgccgcca gtgcacagaa accgagccag gtgattgacg ccgaggccga
    14521 gttttatcgt catggctacg cggcggtgca tcgcggtatt cataccttaa gcgcccaggc
    14581 gaccgagaaa atggagaacg tgcgcaagcg ggcatcgctg tttattaatg cccgttcggc
    14641 ggaagagctg gtgtttgtcc gcggcacgac ggaagggatc aatctggtcg ccaatagctg
    14701 gggcaacagc aatgtgcggg cgggcgataa catcatcatc agtcagatgg agcaccacgc
    14761 taacattgtc ccctggcaga tgctttgcgc acgcgttggc gcagagctgc gtgtgatccc
    14821 tctcaacccc gacggtacgc tgcaactgga gacgctgcct acgctgtttg atgagaaaac
    14881 tcgcctgctg gcaattactc atgtctccaa cgtgcttggc acagaaaatc cactggcgga
    14941 aatgatcacg cttgcgcacc agcatggcgc aaaagtgctg gtggatggcg ctcaggcggt
    15001 gatgcatcat ccggtggatg ttcaggcgct ggattgcgac ttttacgtgt tctccgggca
    15061 taaactgtat ggccccaccg gaattggcat tctttatgtg aaagaagcct tgttgcagga
    15121 gatgccgccg tgggaagggg gcggttctat gatcgccacc gtcagcctga gtgaaggcac
    15181 tacctggacc aaagcaccat ggcggtttga agccggtaca cccaataccg ggggcatcat
    15241 tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg cttaatagca tagccgagta
    15301 tgaacagaat ctgatgcatt atgcgctatc acagctggaa tctgtaccgg atctcactct
    15361 ctatggccaa caaaacaggc ttggcgttat tgcttttaat ctcggtaaac accacgccta
    15421 tgatgttggc agttttctcg ataattacgg cattgctgtg cgtaccggac atcactgcgc
    15481 tatgccatta atggcctatt acaacgtccc tgcgatgtgt cgggcgtcgc tggccatgta
    15541 taacacccat gaagaagtgg agcgtctggt gaccggcctg caacgtattc accgtctgct
    15601 gggataacag ggaggcacta tggctttatt gccggataaa gaaaaattgc tgcgtaattt
    15661 tttacgctgc gccaactggg aagagaaata tctctacatt attgagctgg gccagcgtct
    15721 gccagaatta cgcgccgaag acggaagccc acaaaatagc attcagggct gccagagtca
    15781 ggtgtggatt gtcatgcgcc agaatgcgca gggaattatt gaattgcagg gcgacagcga
    15841 tgcggcgatt gtgaaagggc ttattgcggt cgtctttatt ctctacgatc agatgacgcc
    15901 gcaggatatt gtcaatttcg atgtgcgtcc gtggtttgaa aaaatggcgc tcacccaaca
    15961 tctcacccca tctcgttcac aaggtctgga agcgatgatt cgcgcaattc gcgccaaagc
    16021 cgctgcactt agctaaacta aagggacagc tttcatccgg ttggctcaca ttgccggatg
    16081 gtcaatgttt acactgaccg gcacaggagt gttttggttt caggtgaaca taagcaccat
    16141 ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa cgcgcgtctt
    16201 tgcttacact cacgcttatc ggcgctttta gcgccatcca ggctgcctgg gcggttgatt
    16261 atccgctacc accaaccgga agccgactgg ttgggcaaaa tcaaacgtat acggtgcaag
    16321 aaggggataa aaaccttcag gctattgccc gacgttttga tactgcagca atgttgatcc
    16381 ttgaagccaa taacactatc gccccggtgc caaaacctgg tacgacgata actattcctt
    16441 cacaactgtt attacctgat gcaccgcgtc aggggattat cgttaacctt gcagagctgc
    16501 gcctttatta ttatccgccg ggagaaaata ttgtgcaggt ttatccaata ggtattggat
    16561 tgcaggggct ggaaacgccg gtgatggaaa cgcgtgttgg gcagaaaatc cctaacccaa
    16621 cctggacgcc tacggcaggc attcgtcagc gttcgctgga gcgtggcatt aaattaccgc
    16681 cagtcgttcc tgccggacca aataacccgc taggacgtta cgcactgcgc ctcgcgcatg
    16741 gtaatggcga atacctcatt catggcacca gtgcgccgga cagcgttggt ttgcgcgtca
    16801 gttcagggtg tattcgcatg aatgcaccgg atattaaagc cttgttctcc agcgtgcgga
    16861 cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata ttccgtggaa cctaacggga
    16921 tgcgttatgt tgaagtacat cgaccactat cagcagaaga acagcaaaac gttcagacaa
    16981 tgccatacac actgccagca ggctttacgc aatttaaaga caataaggct gtagatcaga
    17041 agttagtcga taaagcgttg tatcgtcggg cagggtatcc ggtttcggtg agcagtggag
    17101 caactcccgc agccagcaat gcgccttcag tagagtcagc gcagaatggt gaaccagagc
    17161 aagggaatat gttacgcgcg acgcagtagt ggtaaatagc agacaaaaaa atggcgcaca
    17221 atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta gccatgttgt
    17281 ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg gaacgcattg
    17341 cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg tcagaagaca
    17401 gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc aggattaccg
    17461 cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag ttaatctcca
    17521 tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt tgatgggaag
    17581 gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta gataaaaaaa
    17641 ttgagagaaa acaggatgct tccatcggat tcatcttaaa taaagtgaga ttatatagtg
    17701 aaatccgatt tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca
    17761 gggcgcttcg atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg
    17821 gtgttagtag tgccgctcgg taccagtgca ccagaaacca taactacgac gtcacctttg
    17881 tgtgccagac cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa
    17941 gtgatctctt taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt
    18001 ttttcgttgg tggtcagtgc caggatggtg gcatccggga agtatttacg tactgcgcga
    18061 gcagatttac cgccctgggt agcaaccacg atcagcggag catccagttt ttcagcagtt
    18121 tcaacggcac cacggcatac cgcttcggtg atgcgcagtt tacggttgtc attgttgaac
    18181 tcgagacggc tgttcatcac gcggtcggta cgttcgcaga tggtcgccat gatagaaacc
    18241 gcttccagcg ggtatttacc ttttgcggat tcaccagaca gcatcactgc gtcagtaccg
    18301 tcgaggatgg cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc
    18361 atggaatcca gcatctgggt cgcagtgata acgactttac gtgcacggat acatttttcg
    18421 atcatcatct tctgggcgaa gataacttct tctaccggga tttctacacc caggtcgcca
    18481 cgcgcaacca tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt gaggccttcc
    18541 tggttttcga ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca
    18601 cggatttcga taacgtcaga acgcttacga ataaaggaag cagcaacaaa gtctacgcct
    18661 ttttcgcaac caaagatcag gtcctgtttg tctttttcag ccagtgctgg cagagcaatg
    18721 gaaacgccag gcaggttcac acctttgttt tcgcccaggt caccgttgtt cagcacttta
    18781 cagataactt tgttaccttc aatggcggta acttccatac cgatcagacc atcgtcaacc
    18841 agtacggtgt tgccaacaga caggtcagta gtgaaacctt catacgttac cgcaaccatt
    18901 tcgctgttgc cgataacaga tttatcagtg gtgaaagtaa aggtctgacc agctttcaga
    18961 gaaacatcgt taccgccttc cagtttcatg gtgcggattt ccggaccttt ggtatcaagc
    19021 aggatagcgg cggttttacc agttttgctc atcacgttgc gcagattctg aatgcgctga
    19081 ccgtgttctg catagtcacc atgagagaag ttc
//
LOCUS       DAJTRH010000076        18666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTRH010000076 DAJTRH010000000
VERSION     DAJTRH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(139..3654)
                     /locus_tag="OO339_002837"
     CDS             complement(139..3654)
                     /locus_tag="OO339_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5927402.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO339_002838"
     CDS             complement(4107..5369)
                     /locus_tag="OO339_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5927403.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO339_002839"
     tRNA            complement(5567..5642)
                     /locus_tag="OO339_002839"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO339_002840"
     CDS             complement(5748..6455)
                     /locus_tag="OO339_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5927404.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO339_002841"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO339_002841"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5927405.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO339_002842"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO339_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5927406.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO339_002843"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO339_002843"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5927407.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO339_002844"
     CDS             complement(11639..11914)
                     /locus_tag="OO339_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5927408.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO339_002845"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO339_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5927409.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO339_002846"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO339_002846"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5927410.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO339_002847"
     CDS             13874..14200
                     /locus_tag="OO339_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5927411.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO339_002848"
     CDS             14384..15103
                     /locus_tag="OO339_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5927412.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO339_002849"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO339_002849"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5927413.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO339_002850"
     CDS             16442..17404
                     /locus_tag="OO339_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5927414.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18666)
                     /locus_tag="OO339_002851"
     CDS             complement(17505..>18666)
                     /locus_tag="OO339_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR5927415.1"
                     /translation="VSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMV
                     GRVPGSLGHVNVLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSC
                     ITGLLIPPSNVLIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLS
                     ERATFAQACKAFLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREV
                     KWRHLPKLILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILL
                     LINIVLLIVGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVG
                     SALFVGCSISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4843 a         4552 c         4616 g         4655 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttactctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaaca
//
LOCUS       DAJTRH010000077        18052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTRH010000077 DAJTRH010000000
VERSION     DAJTRH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            446..1129
                     /locus_tag="OO339_002852"
     CDS             446..1129
                     /locus_tag="OO339_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5927416.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1173..5835
                     /locus_tag="OO339_002853"
                     /pseudo
     CDS             1173..5835
                     /locus_tag="OO339_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6203..7858
                     /gene="lldP"
                     /locus_tag="OO339_002854"
     CDS             6203..7858
                     /gene="lldP"
                     /locus_tag="OO339_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5927417.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7858..8634
                     /gene="lldR"
                     /locus_tag="OO339_002855"
     CDS             7858..8634
                     /gene="lldR"
                     /locus_tag="OO339_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5927418.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8631..9821
                     /gene="lldD"
                     /locus_tag="OO339_002856"
     CDS             8631..9821
                     /gene="lldD"
                     /locus_tag="OO339_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5927419.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10017..10490
                     /gene="trmL"
                     /locus_tag="OO339_002857"
     CDS             10017..10490
                     /gene="trmL"
                     /locus_tag="OO339_002857"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5927420.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO339_002858"
     CDS             complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO339_002858"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5927421.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO339_002859"
     CDS             complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO339_002859"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5927422.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO339_002860"
     CDS             complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO339_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5927423.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO339_002861"
     CDS             complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO339_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5927424.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13386..13817)
                     /locus_tag="OO339_002862"
     CDS             complement(13386..13817)
                     /locus_tag="OO339_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5927425.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14062..15606
                     /gene="gpmM"
                     /locus_tag="OO339_002863"
                     /gene_synonym="pgmI"
     CDS             14062..15606
                     /gene="gpmM"
                     /locus_tag="OO339_002863"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5927426.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15616..16899
                     /gene="envC"
                     /locus_tag="OO339_002864"
     CDS             15616..16899
                     /gene="envC"
                     /locus_tag="OO339_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5927427.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16903..17862
                     /locus_tag="OO339_002865"
     CDS             16903..17862
                     /locus_tag="OO339_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5927428.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17849..18052)
                     /locus_tag="OO339_002866"
                     /pseudo
     CDS             complement(17849..18052)
                     /locus_tag="OO339_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4548 a         4664 c         4674 g         4166 t
ORIGIN      
        1 aattcactcc ctttacttcg atttaccgct ggttgatatt ctgaaaatgc atttttaccg
       61 aaatagccat ctataaacaa taaaaatata tagaagatat catttgttaa ttaacaagca
      121 tttacgtctc tttttttcgg atttaattgt gaaattttaa tcatcatatt cacaaaatca
      181 attatctgtg aaatttttcc cagacatggt ttctttcata tttgacattt attaataatc
      241 atcattagaa tccacgaaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa
      301 tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt
      361 aatgtaaagc aacatagctc accaataata caatggacaa ctctaccggc attgagcgct
      421 ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca gtgctgtcac
      481 tgtttcagtt ctctgcgcat taacgggatg tgactacatt gaagaaggca agccagaaag
      541 tagtttactg aaacaacaag aagaacataa taataaaatt gtgctacttg aaaaacaaca
      601 agcacagcta aagagtcaac tcgaaactat tcaaaaacaa caaactggca taataaacag
      661 tacaaagact ttaactcacg taattaaatc cgttaaagac caacaaaata cttttatttt
      721 taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaacggtt cggtggcttt
      781 agcgggtcga gtgttatcca ttgacgccac agagaatggt agtgttattc atatctcact
      841 ggtcaactta ttaagtacac ctatctcaaa cattggtttt aatgcgacat ggggtggtga
      901 aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat
      961 gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg
     1021 tgtatcgccc aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt
     1081 cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt
     1141 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
     1201 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
     1261 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1321 gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag
     1381 ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1441 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1501 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1561 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1621 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1681 ccaaagcgga aggagcaaac gccattgccc tggggcgtag cagtgtagct agcggtactg
     1741 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1801 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1861 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1921 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1981 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     2041 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     2101 gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata
     2161 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2221 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2281 tagcaaaaaa ggcttggagg aggggctgca gtagatgttg atgacggtac tgttacagca
     2341 ccaacctaca atttaaaaaa tggtagcaaa aataacgtag gggctgcgct cgctgtactt
     2401 gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc tcatggtact
     2461 agtagcccaa ctgccagcgt aatcaccgat gttgcggatg gcacgatttc agcctccagt
     2521 aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg acgatgtcga agccaacacc
     2581 accaatatca ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg
     2641 aacgaaacta aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc
     2701 aacgttaaag atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg
     2761 aaaaccacca acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat
     2821 attgccacta acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag
     2881 gatgcgctga aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc
     2941 accagcaaaa taaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt
     3001 aacggctctc agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc
     3061 actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg
     3121 ctgaaatggg ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc
     3181 aaaatcacca atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt
     3241 agccagcttt atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg
     3301 aatactgacg gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac
     3361 gtcggcgatg cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg
     3421 ctttgggatg ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc
     3481 gtgatcactg atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc
     3541 tcacaactcc acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc
     3601 aatgcagacg gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat
     3661 gtcggtgatg ccctgaatgc tatcgatacc actcctgacg acgctctgct ctgggatgcg
     3721 gacgccggtg aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta
     3781 atcactaacg tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca
     3841 caactctata ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac
     3901 gctgacggca ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc
     3961 ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggataagac tgccaatggc
     4021 ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct
     4081 aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg
     4141 aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca
     4201 acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta
     4261 gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc
     4321 gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt
     4381 attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact
     4441 agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt
     4501 gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca
     4561 gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact
     4621 acgccaacca agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa
     4681 gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt
     4741 ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc
     4801 gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag
     4861 actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc
     4921 ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat
     4981 gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc
     5041 attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac
     5101 ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc
     5161 gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc
     5221 gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct
     5281 gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc
     5341 gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg ctacgacacc
     5401 aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg
     5461 actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag
     5521 ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat
     5581 aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
     5641 accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact
     5701 tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg
     5761 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc
     5821 ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct
     5881 gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc
     5941 tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc
     6001 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt
     6061 gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
     6121 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
     6181 cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
     6241 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
     6301 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt
     6361 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt
     6421 cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat
     6481 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
     6541 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
     6601 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
     6661 gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat
     6721 gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca
     6781 gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
     6841 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc
     6901 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
     6961 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg
     7021 cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg
     7081 ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt
     7141 aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga
     7201 gtgggtgatc aacattccgg tgccgtacct caataaactg gttgcccgta tgccaccagt
     7261 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
     7321 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
     7381 cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg
     7441 catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact
     7501 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
     7561 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
     7621 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
     7681 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
     7741 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
     7801 tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
     7861 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
     7921 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
     7981 ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg
     8041 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
     8101 aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt
     8161 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
     8221 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
     8281 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
     8341 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
     8401 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
     8461 gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac
     8521 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
     8581 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
     8641 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
     8701 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
     8761 aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
     8821 tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt
     8881 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta
     8941 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
     9001 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
     9061 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
     9121 gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc
     9181 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
     9241 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
     9301 tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct
     9361 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
     9421 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
     9481 tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc
     9541 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
     9601 acaccgtact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg
     9661 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
     9721 cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
     9781 ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
     9841 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
     9901 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
     9961 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
    10021 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
    10081 gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgaca
    10141 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    10201 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    10261 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    10321 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    10381 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt
    10441 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    10501 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    10561 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    10621 gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg
    10681 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    10741 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    10801 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    10861 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    10921 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    10981 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    11041 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    11101 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    11161 ttcaaccact tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag
    11221 catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    11281 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    11341 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    11401 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtga ctgctgcgct
    11461 cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat
    11521 ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt
    11581 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    11641 tttgtacatc catgccctga ccgagcatca taccaaaacg gcggttacgc gactggttgt
    11701 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    11761 cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac
    11821 gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt
    11881 tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt
    11941 tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg
    12001 aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    12061 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    12121 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    12181 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg
    12241 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    12301 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    12361 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    12421 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    12481 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac
    12541 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg
    12601 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    12661 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    12721 ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    12781 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    12841 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    12901 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    12961 atactgcgca atgtaatacc aacgttcaca cgttatttca gcaggggatc cagtccacca
    13021 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    13081 tggggaaccg tggtgcaacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    13141 tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    13201 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    13261 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    13321 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    13381 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    13441 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    13501 tgcatgccag aaccgtctac cacgataacc gatttgtctt tgtgcttctc aagctcacca
    13561 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    13621 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    13681 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    13741 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    13801 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    13861 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    13921 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    13981 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    14041 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    14101 gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg
    14161 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    14221 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    14281 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    14341 tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta
    14401 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    14461 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    14521 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    14581 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    14641 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    14701 ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    14761 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    14821 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    14881 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    14941 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    15001 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    15061 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    15121 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    15181 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    15241 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    15301 gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    15361 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    15421 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    15481 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    15541 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    15601 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    15661 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    15721 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    15781 gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    15841 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac
    15901 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    15961 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    16021 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    16081 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    16141 caaacgcgtg aagaagtcac catgcagcat gctgaactgg aagagaaaca aagcgagcaa
    16201 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    16261 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    16321 gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga agccgcggcg
    16381 aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa
    16441 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    16501 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg
    16561 catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc
    16621 tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg
    16681 caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc
    16741 tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca
    16801 ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag
    16861 ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    16921 aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt
    16981 gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg
    17041 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    17101 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    17161 cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt
    17221 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc
    17281 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat
    17341 ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt
    17401 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac
    17461 atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc
    17521 attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg
    17581 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct
    17641 acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg
    17701 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc
    17761 gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc
    17821 aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc
    17881 gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct
    17941 ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata cccggaagta
    18001 aatatctcgg caatcatccg ttggcgggta agaatatccg gctctttgcg ca
//
LOCUS       DAJTRH010000078        17386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTRH010000078 DAJTRH010000000
VERSION     DAJTRH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(42..773)
                     /gene="rlmB"
                     /locus_tag="OO339_002867"
     CDS             complement(42..773)
                     /gene="rlmB"
                     /locus_tag="OO339_002867"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5927429.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(953..3394)
                     /gene="rnr"
                     /locus_tag="OO339_002868"
     CDS             complement(953..3394)
                     /gene="rnr"
                     /locus_tag="OO339_002868"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5927430.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="OO339_002869"
     CDS             complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="OO339_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5927431.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4063..5361)
                     /gene="purA"
                     /locus_tag="OO339_002870"
     CDS             complement(4063..5361)
                     /gene="purA"
                     /locus_tag="OO339_002870"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5927432.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5465..5662)
                     /locus_tag="OO339_002871"
     CDS             complement(5465..5662)
                     /locus_tag="OO339_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5927433.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="OO339_002872"
     CDS             complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="OO339_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5927434.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYVFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="OO339_002873"
     CDS             complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="OO339_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5927435.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="OO339_002874"
     CDS             complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="OO339_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5927436.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="OO339_002875"
     CDS             complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="OO339_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5927437.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="OO339_002876"
     CDS             complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="OO339_002876"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5927438.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="OO339_002877"
     CDS             complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="OO339_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5927439.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="OO339_002878"
     CDS             complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="OO339_002878"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5927440.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="OO339_002879"
     CDS             complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="OO339_002879"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5927441.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="OO339_002880"
     CDS             complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="OO339_002880"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5927442.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15981..17120
                     /gene="queG"
                     /locus_tag="OO339_002881"
     CDS             15981..17120
                     /gene="queG"
                     /locus_tag="OO339_002881"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5927443.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17103..17156)
                     /locus_tag="OO339_002882"
     CDS             complement(17103..17156)
                     /locus_tag="OO339_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927444.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3945 a         4959 c         4365 g         4117 t
ORIGIN      
        1 aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg ctgacgaacc
       61 gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc
      121 attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct
      181 tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag tgtatgatcc
      241 gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc
      301 gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc tacttttttc
      361 gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg aacgcctgcg
      421 gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc gtcgaggatc
      481 agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc ctgatactga
      541 cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg
      601 agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc
      661 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
      721 gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta
      781 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat
      841 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
      901 caaagcgcat tttgtcagca atctaaccct cttcttttaa agagggtatt gatcactctg
      961 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
     1021 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
     1081 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga caacgcttgc
     1141 cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
     1201 gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg
     1261 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca
     1321 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
     1381 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
     1441 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
     1501 cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1561 gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat
     1621 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
     1681 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
     1741 tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag catggtttgc agcatttctg
     1801 catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt
     1861 taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
     1921 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     1981 gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac
     2041 gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     2101 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
     2161 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     2221 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     2281 cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     2341 acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca
     2401 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     2461 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     2521 cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat
     2581 caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct tcttccggca
     2641 cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata tacggaattt
     2701 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2761 cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga
     2821 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
     2881 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     2941 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     3001 cagcacccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     3061 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     3121 gaccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     3181 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     3241 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     3301 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     3361 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     3421 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     3481 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3541 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3601 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
     3661 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3721 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3781 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3841 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3901 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     3961 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     4021 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
     4081 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     4141 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
     4201 tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     4261 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     4321 agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     4381 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     4441 acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     4501 agtttcatca aacagttcgg ccgggaaagg acctgcacct acacgagttg agtaagcttt
     4561 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4621 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4681 cgtaccctgc gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4741 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4801 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4861 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4921 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     4981 gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agagcaacgt gataatcaag
     5041 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     5101 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     5161 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     5221 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     5281 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     5341 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     5401 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     5461 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     5521 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5581 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5641 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5701 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5761 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5821 tgccagagaa gctgttctca taagcacgca ggctacggat gaatacgtag aagtccggat
     5881 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcataa
     5941 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
     6001 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     6061 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     6121 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     6181 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg
     6241 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     6301 cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtccagg cgaccaattt
     6361 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     6421 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     6481 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6541 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag
     6601 gctcataaac caaaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6661 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6721 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6781 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6841 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6901 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     6961 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     7021 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     7081 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     7141 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggaata
     7201 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     7261 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     7321 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     7381 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     7441 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     7501 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7561 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7621 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7681 gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7741 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7801 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7861 tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7921 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     7981 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     8041 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     8101 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     8161 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     8221 gctgataaaa acgactcctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     8281 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     8341 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     8401 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     8461 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     8521 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8581 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8641 caccgacatc tgcaacatca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8701 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8761 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8821 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8881 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     8941 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     9001 gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa
     9061 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     9121 ggctcagggc atggtcaaaa agaacgaccg aagcacccat cgctttgaca gcttccgcaa
     9181 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     9241 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     9301 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     9361 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     9421 ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     9481 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9541 tgttactgtg atgagaaacc gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa
     9601 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
     9661 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9721 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9781 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9841 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
     9901 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
     9961 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    10021 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    10081 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    10141 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    10201 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    10261 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    10321 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
    10381 tctgccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
    10441 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    10501 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10561 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10621 gcgttcggct tcgccgtccc gatatccatc cctttgtaaa taaggacaga atcaacgctt
    10681 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10741 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10801 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10861 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10921 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    10981 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    11041 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    11101 aatacccaat tccgctaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    11161 aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt
    11221 caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc
    11281 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    11341 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    11401 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    11461 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    11521 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11581 cgggatggca cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg
    11641 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11701 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11761 cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg
    11821 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11881 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc
    11941 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc
    12001 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    12061 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    12121 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    12181 gtagaacaga tccagcacct ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt
    12241 cacgtccatg tcgcgtcctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    12301 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    12361 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    12421 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    12481 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12541 ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12601 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12661 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    12721 catcggatgc gcaaggaagt aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    12781 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12841 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    12901 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    12961 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    13021 agactgactg ttcgccagca catcacccgc cccacccagc agctccgaca gtttctcgtg
    13081 ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca cccatacgga
    13141 agcgccagtc gcactacggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    13201 gttttgctta tgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    13261 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    13321 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    13381 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    13441 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    13501 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13561 tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac
    13621 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag
    13681 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac
    13741 cagattattg ccgctgaaca acaacggcag tccctgaatc atgcccgttt gtttgatatc
    13801 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    13861 caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc
    13921 acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat
    13981 catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga
    14041 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    14101 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    14161 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    14221 caaatcaaag tgatagacca ttaagttgtc gagcgtatag ggttcgacca gcgtataagt
    14281 ggggcttttg acattaccct gatgacccag agcctgtaaa aagccccggc taaaggtggt
    14341 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    14401 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    14461 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    14521 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg
    14581 cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    14641 taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa
    14701 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    14761 cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag
    14821 cgacggaaca gcctaacaac cgtgcggcct cgctaggatg cggcgtgatc acgcgattgt
    14881 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    14941 tgcgaaaatt ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg
    15001 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt
    15061 catgcaccat caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca
    15121 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    15181 ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag
    15241 tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    15301 tctcctgacc tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa
    15361 catcccgcgc ttttccagtg agcaagccta gtttcagcgc aataaaagtg atggtgtgat
    15421 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    15481 caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    15541 cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt
    15601 cgggccagac aatattcgaa gcatggatct cgccacccgc gtttaaccat gcttcgcgtg
    15661 ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac gtgacctcaa
    15721 tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    15781 cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    15841 cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcacatct gccgcctcgc
    15901 gttctccgcg gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt
    15961 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    16021 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    16081 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    16141 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattaccc ggtacgctgc
    16201 gtgtaatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    16261 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    16321 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    16381 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc
    16441 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt
    16501 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    16561 gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    16621 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact tgaaggggcg ctcccggaag
    16681 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    16741 cgtggaatcg ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    16801 acgcaccgga actcattgag ttatttgcct ggagcgaaga gaagttttta aaagtcacgg
    16861 aaggatcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    16921 taggcaatgc cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc
    16981 acccacttct cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg
    17041 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag
    17101 ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    17161 attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacggaatct tgatttttaa
    17221 cttatttatc aataggttaa aaaaatacca ttcaccgtgc gaagtttctt tgaaattaag
    17281 attacagcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac
    17341 cagcaataac gcatcgcgtc gctgtagata ttttattgag agaaga
//
LOCUS       DAJTRH010000079        17090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTRH010000079 DAJTRH010000000
VERSION     DAJTRH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..508
                     /locus_tag="OO339_002883"
     CDS             <1..508
                     /locus_tag="OO339_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5927445.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO339_002884"
     CDS             complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO339_002884"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5927446.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1778..2866
                     /gene="hcxA"
                     /locus_tag="OO339_002885"
     CDS             1778..2866
                     /gene="hcxA"
                     /locus_tag="OO339_002885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5927447.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2876..3073)
                     /locus_tag="OO339_002886"
     CDS             complement(2876..3073)
                     /locus_tag="OO339_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5927448.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO339_002887"
     CDS             complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO339_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5927449.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO339_002888"
     CDS             complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO339_002888"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5927450.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO339_002889"
     CDS             complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO339_002889"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5927451.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO339_002890"
     CDS             complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO339_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5927452.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO339_002891"
     CDS             complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO339_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5927453.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO339_002892"
                     /pseudo
     CDS             complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO339_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10661..11617
                     /gene="fepB"
                     /locus_tag="OO339_002893"
     CDS             10661..11617
                     /gene="fepB"
                     /locus_tag="OO339_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5927454.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO339_002894"
     CDS             complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO339_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5927455.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12964..13968
                     /gene="fepD"
                     /locus_tag="OO339_002895"
     CDS             12964..13968
                     /gene="fepD"
                     /locus_tag="OO339_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5927456.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLFIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13965..14957
                     /gene="fepG"
                     /locus_tag="OO339_002896"
     CDS             13965..14957
                     /gene="fepG"
                     /locus_tag="OO339_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5927457.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14954..15769
                     /gene="fepC"
                     /locus_tag="OO339_002897"
     CDS             14954..15769
                     /gene="fepC"
                     /locus_tag="OO339_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5927458.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OO339_002898"
                     /pseudo
     CDS             complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OO339_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3994 a         4862 c         4442 g         3792 t
ORIGIN      
        1 ctttcgtgaa aagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa
       61 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga
      121 aatagatggt tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat
      181 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt
      241 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
      301 tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg
      361 tgaacttagc cagttgggat gggatgataa taaaattggc aaagagctgg gaatggacag
      421 cgacgaagtg ctgcgactga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca
      481 atattcccgc gcctggacgg ttaaatagct aaagctggcg caggcgttca gctgctgcca
      541 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg
      601 cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa
      661 actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
      721 cacacggtaa aatctccagc cggctttcat ttaaagcatt caccagaata tcgcgcttct
      781 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa
      841 gcgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt
      901 cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg
      961 tcttgccaaa tgaagaaacc gccactgccc gctcacgcag ctgcggatgc gccagcacac
     1021 tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa
     1081 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     1141 cactggggtt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg
     1201 caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgt ttcactattc
     1261 ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt
     1321 catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg
     1381 ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag
     1441 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     1501 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     1561 gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg
     1621 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     1681 ctgacgaagt tgcgtgttga aggcactata acacgatgcc agtatttggc aatcaagacg
     1741 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     1801 ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacaattt
     1861 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc
     1921 ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg
     1981 tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag
     2041 cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg
     2101 tctcggtctg ccatttgttg ccgctccgac gatcgccgcc acctgcgcag cctggacacc
     2161 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     2221 caattttatg gtgctggtgg aaccggagat tatcctcaat gcaccgcaac aatatctgct
     2281 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     2341 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     2401 cttgttaaac agtagcgaac aggcacttgc cgatcagcaa aatcagcagt taacgcaatc
     2461 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     2521 tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca
     2581 aaccgagaag tttcttcacg gcaccaaagt cgcttacgga attctggtgc aaagcgcctt
     2641 gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc
     2701 gactacgctg gcggagctgg aggtggatat caataatcag gcggagatcg acaaagtgat
     2761 tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga
     2821 tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc
     2881 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct
     2941 cgtaggtcat cggcgtttga tcgggatgat taacccgcat atgttcgaca tagttgtcgt
     3001 aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca
     3061 gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt
     3121 gaacgcttta tccggcctac aaatgctatc aacgcactcg gtcagtcccc tcgccccatc
     3181 ggggagaggg ttagggtgag ggggaaccct cggcactatg ccaacattcg ccaacatccc
     3241 ccctcactct gactttagtg cgcgcctttc gcctgcgcta cgatctcctc gacattttcc
     3301 ggcatcggct catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgcaagt
     3361 gccgtcttaa tcgagaacag tgccaggacc accacgacca ccataaagaa gatggttaac
     3421 ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac
     3481 ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatgaccag gaagccgact
     3541 ttcgcatccg ggctaaacgc tttcagccag tcagcggtca gggtacaaat cagcagccag
     3601 gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg
     3661 gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac
     3721 agagtgttaa taccgcccag cggatcgacc acgctctgat ggaggaagta gccccacgcc
     3781 agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca
     3841 ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct
     3901 accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca
     3961 tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc caacgtcggc
     4021 gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc
     4081 gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca
     4141 tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc
     4201 ggatcgatga tacaggcaga aactagcgcc ataatcgcca cgaaggattc catcaacatc
     4261 ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg
     4321 gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac
     4381 gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc
     4441 atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt
     4501 ttcaggaagg tcgagagata gtcacgcggg gccagcagca accacaccgg cagcaccgcc
     4561 gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac
     4621 ggtgcccagg tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaac
     4681 accaggccaa tcactgacac ttcgccaatg cggcccggac gcagatagcg caggtagatt
     4741 cccataaaaa tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga
     4801 gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag
     4861 caggctacca gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc
     4921 agcgagcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg
     4981 gcgagaacca ccccggcgag cagccagatc atccccggca ggtagcccat ttgcgctgcc
     5041 agcaccggcc ccaccagcgg ccctgctccg gcaatggccg caaaatggtg accgaacagc
     5101 actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata
     5161 cgcgtcgggt caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg
     5221 atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga
     5281 tttaaagcaa tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg
     5341 tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt
     5401 tgtttctata acatctttgt acgatttaac aacacggtaa ccatataaat cggagttgaa
     5461 accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat
     5521 ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa
     5581 cgccgcccct gttcatcgaa aacgacgatt tcccaactct gattttgcct cccaagatgc
     5641 agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta
     5701 agttccgtgc ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc
     5761 agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta
     5821 cgggtatcaa ccggcatttc ggcttccagc acatcatctc ccagaagggt atacacaatt
     5881 cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg
     5941 cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta
     6001 gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg
     6061 gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac
     6121 gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc
     6181 caggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg
     6241 ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata cgcgccgtgt
     6301 gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca
     6361 tggtttgctg gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt
     6421 cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgatcagca
     6481 cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcccggcgt
     6541 cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa
     6601 aacctgtaac tttcgctccc gcctcaacaa acgtcagcgc cgtggcgtag ccgatacctt
     6661 tacctgcgcc ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga
     6721 gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc
     6781 accatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta
     6841 gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac
     6901 ctcacgcagc gccgctttgc tggcggggat aggtgctggc agtaattctt cggtcatcac
     6961 cacccggcca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa
     7021 atcagccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc
     7081 ggtggtcata cagccaatgt gggcatatac tcctgtaata atcagctggt tacgtccact
     7141 ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag
     7201 caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg
     7261 ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt
     7321 cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc
     7381 aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata
     7441 gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt
     7501 attctgcgga atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat
     7561 cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat
     7621 cgactttccc gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt
     7681 taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct
     7741 cttttaccac cagataagca cagctttttt cgcccatcag ctcattttcc atgctcacca
     7801 gtgcggcata gatcactgcc ggatggctca gcagcaggtt ttcgatatct tcggcagcga
     7861 tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct
     7921 ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg
     7981 cattgtgctg cggacttttg taatagccac ggaaggtgta cggcccgcgc gtcatcaggc
     8041 gtccaacttc cccttgcggc agtggatttc cttcggcatc ggcaacccac acttcatcgt
     8101 ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg
     8161 tgtagttcac cagcccttcc gccatgccaa acacctgctg caactggcag ccaatctcag
     8221 cgggaatacg cgctgcaagc gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca
     8281 gcgaggcaag ctgcgcccgg ctttcgcctt cagccagcgc ctgcaaccac aggctgactg
     8341 ccggcggcac cagcgcggta acgttaacct gatgttttgc aatcaacggg aagcaaagcg
     8401 tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc
     8461 ctggcgaact catagtgtag ttatgagccg ccgggatcga gcacaggtag cgcgtctgtt
     8521 gtgtgaactg acaaatctcg gcgctacgac gcacgctgta gtaatagtcg ttatgagtgc
     8581 gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat
     8641 cagcaggtga tggcgtggca gtaaaaccct ctgccggatg gttaatcgca tcctgcaagt
     8701 tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat ggaagaatgc tctgcaacaa
     8761 atgtgttgag gaaatcatcc ccgctaaata gcgcatgctg gcgatcggca atcagtaacg
     8821 ccggttcaat ctggctggcg taggcgttca gttcactacg ctgatggctg aacagcgcca
     8881 gcaccggcgc gacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt
     8941 tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgc aaactacagg
     9001 cgaggttatc cgccgcctga ttcagctccc gataactcaa ctgccgctcg ccgtcgataa
     9061 ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagtggc aaatcctgcc
     9121 agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc
     9181 tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg
     9241 tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac
     9301 gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt
     9361 cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc tccagttcag
     9421 caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca
     9481 gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga
     9541 gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac
     9601 tgcgttcgcg cagtacctct ttcatcgcct gagtccccag ttcatgttca tggcgatctt
     9661 tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac
     9721 gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt
     9781 ccgggctggc ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa
     9841 ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg
     9901 tgatatcaat caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg
     9961 cggcgcgggc acccatctgt tcaaacgtgg tttgttcagg aattgcctag cgttccacca
    10021 cgttcagcga ctggctgcgg gtaaaacagc gggctgaggt ctgtttttcc tgacgggaga
    10081 acgactgcta ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg
    10141 ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga
    10201 gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaaacggg
    10261 cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg
    10321 caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca
    10381 caaaatgata aaggctttaa taatgattat cacttatatt ttcggccgct aacctaaaag
    10441 caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg
    10501 ttgtggttac aatgaaaatg agaagcatta ttgatgaatt cgcataagcg caatgtgatg
    10561 tcctgcgccg ttttgccccc tctccccaaa gggacggatt gtgcgctttg tcgaatttgt
    10621 cattacgccc ttaaccttat taataacagg aagctgactt gtgagactcg ccccgctcta
    10681 ccgcaacgcc cttctattaa caggactttt gctttcagga atagccgtag ttcaggccgc
    10741 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    10801 gcgtattgtt tccaccagcg tcaccctgac cggatcactg ctggcgattg atgctccggt
    10861 gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgca gatgaccagg gctttttacg
    10921 ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag
    10981 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    11041 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    11101 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    11161 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    11221 caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa
    11281 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    11341 gaaattgccc gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    11401 gcttggtggg gaaaatctgg ctgcggggtt aaacggtgaa tcgctattcc tgttcgctgg
    11461 tgatcagaaa gatgccgatg cgatttatgc caatccactg ctcgcgcacc tgcctgcggt
    11521 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    11581 catgcaagtg ctggaacggc ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    11641 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    11701 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    11761 ccaaaccacc cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    11821 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    11881 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    11941 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    12001 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    12061 cgccgagcgg gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt
    12121 cagccagcgc cggatacagt accagcaccg cgctcgccat cgtcaataaa ccacccagca
    12181 gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcaagcg
    12241 gctgcggtgg cggtggcaac tccggaaggc ttaataacgg taacaaggta ataaacgtgc
    12301 ccgccgccgc cagcccgtag ttccaggcta cgccgccggt cgccagcaat aaaccgccaa
    12361 tcatgggcga aatcaccgac cccaggcgca cggtcaacat ggtgatcgcc ccggcctgca
    12421 ttaagttttc acgccctacc agtgcagatg ttgccgccag tagcgccgta acaccaagtg
    12481 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    12541 gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    12601 tcactttttt gcgttcatag cgatccgcca gcacaccgcc aaccatcagg ccaacaaaca
    12661 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgg gtcatcatct
    12721 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag
    12781 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    12841 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    12901 acatgttaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    12961 cgtatatctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    13021 ctgattatcg cgacggcatt aagcctgttc attggggcaa aatcactccc cgcttccgta
    13081 gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    13141 cggctgccgc gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg
    13201 ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc
    13261 ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc cgcacaggaa
    13321 caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc ctttaccggc
    13381 agtcagggtg gagggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg
    13441 gcggtgctgg aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa
    13501 ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    13561 ctgatcccgg ttctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    13621 ttgagcctcg gcagtgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg
    13681 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt
    13741 gcctttatcg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc
    13801 tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc cgatatcatc
    13861 gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca
    13921 ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    13981 gccgattact catcacctgt ttgctgctga tttccgcctg tgtggttgca ggtatctggg
    14041 gattacgcag cggtgccgtc acgctggaaa tctcgcaggt attcgccgcg ttgatgggcg
    14101 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    14161 cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg ctgatgcgta
    14221 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    14281 tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca atggtgggcg
    14341 gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggtatc gacacctttc
    14401 ggttgattat tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    14461 tgctgaaagc gtctttagaa acggcgctaa cagcaggttt gtggaatgcc ggatcgctca
    14521 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    14581 ccgccacctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat accgcctgcg
    14641 cgctgggcgt cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    14701 ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca
    14761 ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg ctgtgcggag
    14821 cgctgttact gctggcggcc gttctctgcg cccaacaact gtttatgccg tatcaacttc
    14881 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    14941 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg
    15001 atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac
    15061 ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct taagccgcct
    15121 gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag
    15181 taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat
    15241 caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg
    15301 gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct
    15361 ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat
    15421 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    15481 cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    15541 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    15601 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    15661 gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag ccggaatgcc
    15721 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    15781 tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca cgctaccaca
    15841 agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc
    15901 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    15961 gacatttgct tttgttaatt gctcgacaag atactgccga ttacgtaatt caccgttcag
    16021 ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc
    16081 gagggaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt
    16141 tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    16201 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    16261 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    16321 atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg taaaacttag
    16381 cgtccaggat gtatacagtg acggttcatc ttttttctta ctagcattgt catcaaccgc
    16441 tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat cgattttcgc
    16501 ctcttttaat tagtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    16561 agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat taacatcgag
    16621 atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa tggcttcggc
    16681 gggagtaata acggctgagc tggtccattt ttgcggcagg atgaaagaga tcagtaaacc
    16741 tgcgcaggca aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaacttc
    16801 gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat actcagaaaa
    16861 atgagcgtca cttccctgtt taatattcaa tgatgacatg gggtataacc tgaaaatgag
    16921 tccaataggg gaaaatctcc acggcgctca ctttaccagt tggtgagaat tatccgaagc
    16981 tgaagtttgt aaattccttc cactgaatgc aacattcact ctatttaaga gaaatattca
    17041 tcaaacctat aaatcaaaca aataaaaaac ccataattat tttctgtaat
//
LOCUS       DAJTRH010000080        17087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTRH010000080 DAJTRH010000000
VERSION     DAJTRH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            446..1012
                     /gene="mntP"
                     /locus_tag="OO339_002899"
     CDS             446..1012
                     /gene="mntP"
                     /locus_tag="OO339_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5927459.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1009..1818)
                     /gene="rlmA"
                     /locus_tag="OO339_002900"
     CDS             complement(1009..1818)
                     /gene="rlmA"
                     /locus_tag="OO339_002900"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5927460.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1984..2193)
                     /gene="cspE"
                     /locus_tag="OO339_002901"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1984..2193)
                     /gene="cspE"
                     /locus_tag="OO339_002901"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5927461.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2206..2349)
                     /locus_tag="OO339_002902"
     CDS             complement(2206..2349)
                     /locus_tag="OO339_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5927462.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3020..3307)
                     /locus_tag="OO339_002903"
     CDS             complement(3020..3307)
                     /locus_tag="OO339_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5927463.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3382..3525)
                     /gene="mgrB"
                     /locus_tag="OO339_002904"
     CDS             complement(3382..3525)
                     /gene="mgrB"
                     /locus_tag="OO339_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5927464.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3684..3923
                     /locus_tag="OO339_002905"
     CDS             3684..3923
                     /locus_tag="OO339_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927465.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4066..4857)
                     /gene="kdgR"
                     /locus_tag="OO339_002906"
     CDS             complement(4066..4857)
                     /gene="kdgR"
                     /locus_tag="OO339_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5927466.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5034..6407
                     /locus_tag="OO339_002907"
     CDS             5034..6407
                     /locus_tag="OO339_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5927467.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6453..7334)
                     /gene="htpX"
                     /locus_tag="OO339_002908"
     CDS             complement(6453..7334)
                     /gene="htpX"
                     /locus_tag="OO339_002908"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5927468.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7526..9574)
                     /gene="prc"
                     /locus_tag="OO339_002909"
     CDS             complement(7526..9574)
                     /gene="prc"
                     /locus_tag="OO339_002909"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5927469.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9594..10292)
                     /gene="proQ"
                     /locus_tag="OO339_002910"
     CDS             complement(9594..10292)
                     /gene="proQ"
                     /locus_tag="OO339_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5927470.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10389..10886)
                     /gene="msrC"
                     /locus_tag="OO339_002911"
     CDS             complement(10389..10886)
                     /gene="msrC"
                     /locus_tag="OO339_002911"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5927471.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11016..12299
                     /gene="yebS"
                     /locus_tag="OO339_002912"
     CDS             11016..12299
                     /gene="yebS"
                     /locus_tag="OO339_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5927472.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12268..14888
                     /gene="yebT"
                     /locus_tag="OO339_002913"
                     /pseudo
     CDS             12268..14888
                     /gene="yebT"
                     /locus_tag="OO339_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14968..16407
                     /gene="rsmF"
                     /locus_tag="OO339_002914"
                     /pseudo
     CDS             14968..16407
                     /gene="rsmF"
                     /locus_tag="OO339_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16525..16761
                     /locus_tag="OO339_002915"
     CDS             16525..16761
                     /locus_tag="OO339_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5927473.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16866..17057
                     /locus_tag="OO339_002916"
     CDS             16866..17057
                     /locus_tag="OO339_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5927474.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
BASE COUNT         4138 a         4302 c         4125 g         4522 t
ORIGIN      
        1 catatcaact caataactta tgaatttagc caaagctatg tttagtgtat ttttaataat
       61 cagacatagc ttaggctata ttatctcttc ccttatttgt tatttatttt aacgtttcat
      121 tgatatataa acataaatga aaatcgttat caataaagca atgaaataat atattccaac
      181 agttgtttta tattctcaaa atatgttaag gttgcgccct catttgggga gtagccgatt
      241 tccagattcc ggaaatgtac gtgtcaacat actcgttgca aaacgtggca cgtacggact
      301 gaagactttc agtcaggcga gaccatatgc acatcaatcg ctatgcctgc atgaacgcga
      361 aaacccatcg cggcttgttg ttcatgtatg catgtttgct gggggcgatg atgtgtttta
      421 tggatacccc ggtcaggacg ttgtcatgaa tatcactgct actgttcttc ttgcgtttgg
      481 tatgtcgatg gatgcatttg ctgcatcaat cggtaaaggt gcccccctcc ataaaccgaa
      541 attttctgaa gcattgcgaa ccggccttat ttttggtgcc gtcgaaaccc tgacgccgct
      601 gatcggctgg ggaatgggca tgttagccag ccggtttgtc cttgaatgga atcactggat
      661 tgcgtttgtg ctgctgatat tcctcggcgg gcgaatgatt attgagggtt ttcgtggcgc
      721 agatgatgaa gatgaagagc cgcgccgtcg gcacggtttc tggctactgg taaccaccgc
      781 gattgccacc agcctggatg ccatggctgt gggtgttggt cttgctttcc ttcaggtcaa
      841 cattatcgcg accgcattgg ccattggttg tgcaaccttg attatgtcga cattagggat
      901 gatggttggt cgctttatcg gctcaattat tgggaaaaaa gcggaaattc tcggcgggct
      961 ggtgctgatc ggcatcggcg tccagatcct ctggacgcac ttccacggtt aataagaacg
     1021 ctgccagagg tgaatattaa agtccgtctg gcagtcgaac acttcttttg ctgccagtgt
     1081 ttgccagact tctggcttcg cacgccaggc aaacggcgtc atctgcaata atgcgacggc
     1141 ttcatcaccg cgaagacgca tcggataaca caactccgca ctctgctgta atgtaaaacc
     1201 ttccagttgt tctgcatgag gtgcatgaag atgtacttca ttgtaaatca gccccttcag
     1261 ctccatcaaa tgtcgcggtc ccggcgtggc agtaatgacc cagccgccgg gcttcactac
     1321 tcgtgctaat tcttctgctt tacacggcgc gtaaatacgt attatggcgt ccatactggt
     1381 atcggaaaac ggcaaacggt ggctggaagc gacacaaaga gtgacctgcg gatagcgttt
     1441 cgccgccgct tttatcgcta ccttcgagac atccaggcca aacgtggtga tttcgggcaa
     1501 cgcatcggca aatgcgtgtg tgtaataccc ttcaccacag ccaatatcca gcaccgccgt
     1561 ggccttttca tcaagccgtt cccgcagttg ggcgacaatt gcatcacgca gcggctgata
     1621 atgtccggca tctaagaatg cgcggcgtgc ttgcatcatt tccgcgctgt cgcccggatc
     1681 acgagaccgt ttatgctgaa cgggcagcag attgacatac ccttctttcg ccatatcaaa
     1741 ctgatgtcgt tggggacaga tatagctgtt tttttcacgc gaaagaggct gatggcaaag
     1801 tggacaggaa aacgacatga caactccggc aggatactaa aggccgcaag tgtaacgcga
     1861 atcacgcggc gggcaaatat ccgaacgcca ggagcgaaga taaagcatag taaaaagcct
     1921 cgcattcgac gaggctttat agcgagattg aagcgtattc acacttcaga tcagtggatt
     1981 cgatcagata gctgttacgt taacagctgc cggacctttc tggccgtcct gaatttcgaa
     2041 ctcaacgttc tgaccttcag ccagagtttt gaagccatta ccctggatag cggagaagtg
     2101 tacgaacaca tctttgctgc catcagccgg agtaatgaag ccaaaacctt tagactcgtt
     2161 gaaccactta acctgacctt taatctttgc catttgaaaa attccttaga ttgttttctt
     2221 cgcccgcagg cataacatag ataaaactga cacattactg catgaggcac caatataagg
     2281 ctcggcagag aagcggtatt caacgtcaac gtgtttactc aggacttctt tactgaaaat
     2341 gccacacata aacagaactg tacctcgttt aacccgaaat cttttatcac atacaacgtt
     2401 aattatggca agccattttt aaacatatct cgatcagacg cacaaatctc gatacgtctt
     2461 ccactttttt tgcacactta tgcaacggaa tacgcgccga tatatcattg tgcttaacct
     2521 tgccagttca ggcagatact taaactggcg tagtttctaa catagttcaa taagttcagt
     2581 tgttccaggc cagagggaga agttattaca tagtgcgtgc aatatcacat tttctgctat
     2641 gcaatgaata aaaagttata tcactttttc tcataaaaca gtcagttaac ggctatcaat
     2701 taccctaaag agaagtcaat ccccaaaggg attgtaaatt taaaataaga aaaattgatg
     2761 agtgagcaaa aaaaatcaag agagaaacgt ttcgctaata aataatacca cgcttaatca
     2821 caaatctgat gtaaacatcc taataaatta atgaggaaaa tatctttttc tgatatcccg
     2881 ttacgcaaat aaataatatg ataattattt gaagtcagac cagagtttta atttgaagca
     2941 gttgcaccat gacagtgcgt ataatcagga tgaattgagt aacccttcaa cgacggggtt
     3001 actcaagatt gaggaaggat tatcgttcgg cgaccaggcg aataatatct tcgtcatcaa
     3061 ccgatttttg actctcaatg gtctttttat ctgctttctc tggttcgttt gcttcgcaca
     3121 aacgacgtaa cagtgcattt tgacgttttt gctgatccag caacgcctca agcagttcaa
     3181 tctgttcgtt agtccgtgaa ctggcacgat tgataaaaaa ccacaagatg agcccgataa
     3241 gaagaaccac caccgataca accaaagacg caatgttcat cacgcctgaa tttacaactt
     3301 cgttcatttc accacctctg agtagaggcg ctattctacc actgctggag aggaagaaaa
     3361 tctagtgctg aaaaaatgat atcaccacgg gataaactgg ttaatggcac aaattccgct
     3421 gaaaaattgt acatcctgat cgcacatcat gttgaatacc tgcgcccaaa gcagcaagca
     3481 agccaacacc acgacaacca gaacgaccca tcgaaacttt ttcactccaa tctccgcttc
     3541 acctacctta tgtcattaaa ctagcatgcg ttagttaaac agcgcctaac tatgtcgata
     3601 ttcgtgcttt ttcggaatga gtagcttgct tcagcgcata ttgccgctac gattaagcga
     3661 acataaaaag agaagagatt gtaatgcgat tcatcattcg cacagttatg ctgatagcac
     3721 tggtatggat aggattatta ctcagcggat atggcgtttt gattggaagc aaagagaatg
     3781 ccgcaggatt aggtttgcaa tgtacctatc tgactgccag aggcaccagc acggtacaat
     3841 acttgcacac taaaagtggt ttcttggaga taacagattg cccactgtta cgtaaaagca
     3901 atatcgtggt cgataatggt taagggtaat aaataaccag attctcactc atgagtaaaa
     3961 tattcttata gcaagcgcgt ttaatataac gcaccttcaa ctttattttt ctcatgctaa
     4021 ataaagaacc tgtctgtttg atattccgac aggttcttac tctcatcaga acggatagtc
     4081 gtgataaccc atttgggcag aaattttgcg cgctgcggta tgcaacattg cgacatattc
     4141 ctgtaaacgc tcttcagaga aacgcaacgt cgggaaggag atgctcaaac cggcaatgac
     4201 cacgccaaag cgatcaaata ccggtaccgc aatgcattgc agcccttctt cctgctcttc
     4261 gttatcttct ccatacccct gctcgcgcac ctggtccaga acgggtaata acgcttctgt
     4321 actggtgatg gtccgctcgg tactgcgttt atactccacg ccctcaagaa tttgcttcac
     4381 ttcatcgcga tcgcgccatg ccagcagtac cttaccaatc gcggtgctgt acagcggatt
     4441 acgacgccca atccgtgaat acatgcgcaa attgtacata gagtcaattt tgtgaatgta
     4501 aacaatactg tcttcgtcca gtgcgccgag gtggatagtt tctttggtca ggcgggagat
     4561 ctcacgcatc tggatatctg cgctacgaat taaatcgacg ttttgtaacg cgcgagcgcc
     4621 cagttcaaac aatttcaggg tcagggaata tttctccgac tccccttcct gcgccacata
     4681 acctaaggtt ttcatggtct gtaaaaagcg ataaacggtg ctttttgaca tcatgacgcg
     4741 ctgcgacagc tcggttatcc ctatttcgcg ctcttcaccc agcgcctgca aaatgccaaa
     4801 aacttttagc acggaagata cagaatcagg ctgtttatcc agatctgcgt tagccattta
     4861 tcacctcatt gcgagtgttt tataaaaatc agaactgttt tttattataa tttcgcacca
     4921 gggtggtcgc aatccatctt ttgccggtta gttacaattc tgcgacatcc accgtgaata
     4981 tcagtgctag aatcataccc ctgttgatta ttcaccaaag atataaaatt cctatgccaa
     5041 aagttcaggc cgacggcctg ccattgcccc agcgatacgg tgcgatatta accattgtga
     5101 ttggtatttc gatggctgtc cttgacggcg caatcgccaa cgtcgccctg ccaacaattg
     5161 ccacggacct tcatgccaca ccagccagtt ccatctgggt agtgaacgcc tatcaaatcg
     5221 ccattgtcat ctccctgctc tcgttttcgt ttctgggcga tatgtttggc tatcgacgta
     5281 tttataaatg cggtctggtc gtttttctgt tgtcttcact gttctgcgcc ctttctgatt
     5341 cgctgcaaat gctcaccctt gcgcgtgtca tacaaggttt cggcggtgca gcgttgatga
     5401 gcgttaatac cgcacttatc cgcctgatct atccacaacg ttttctgggg agagggatgg
     5461 gcataaactc gtttattgtt gccgtctctt ctgctgccgg gccgacaatt gctgcagcaa
     5521 tcctctccat cgcatcctgg aaatggttat ttttaatcaa cgtaccgtta ggtattatcg
     5581 ccctgcttct ggcgatgcgt tttctgccac ccaatggttc tcgcgccagt aaaccccgtt
     5641 tcgacctgcc cagcgccgtg atgaacgcgt taaccttcgg cctgcttatc actgcgttga
     5701 gtggtttcgc tcaggggcaa tcgctgacgt taattgctgc ggaactggtg gtaatggttg
     5761 ttgttggtat tttctttatt cgccgccagc tttctcttcc cgtaccgctg ctaccggtgg
     5821 atttactgcg tatcccgctg ttttcacttt ctatttgcac atctgtttgc tctttctgcg
     5881 cacaaatgct ggcaatggtt tccctgccct tttacctgca aaccgtgctc gggcgtagtg
     5941 aagtcgaaac aggtttactt ctgacaccgt ggccgttagc aacgatggtg atggctcctc
     6001 tggcaggcta tttgattgaa cgcgtacatg caggattgct gggtgcttta gggttgttca
     6061 tcatggctac ggggcttttt tccctggttc tgctgcccgc gtcacctgcg gatatcaata
     6121 ttatctggcc gatgatctta tgtggcgctg gatttggctt attccagtca cccaataacc
     6181 acaccattat tacctccgct ccgcgcgaac gtagcggtgg ggccagtggc atgttaggaa
     6241 cggctcgtct actgggtcag agtagtggcg cggcgctggt ggcgctgatg ctaaatcagt
     6301 ttggtgataa tggtacgcac gtctcgctga tggctgcggc tattctggca gtgattgctg
     6361 cctgtgtcag tggtttacgt atcactcagc cacgagccag ggcataaaaa aagcgcgtcg
     6421 atcaggacgc gctttttagt atttacttca tattacttca ggtattcacc cgtacgcaga
     6481 gcttcaatac gtttatccag cggcgggtga gtcatgaaca actcactgag cgatttcgac
     6541 ttaccgttaa tgcagaaagc catcatgctg gttgcttctt gcggttcata gctggttttc
     6601 aggcgctgca gcgcggcaat cattttctcg cgaccaacca gttttgccga accggcatct
     6661 gcatggaatt cacgatgacg cgagaaccac atggtgataa tgctcgccag aataccaaac
     6721 accagttcca gaaccgttgc aaccgcaaag tagatcagcg ggttgccgtt gctctcttca
     6781 ccttcatcac gatttccgcc cataaaaccc gcggcaagct gcgccagaat acgggaaata
     6841 aagataacga aggtgttcac cacgccctga atcagcgtca tggtgaccat atcaccattg
     6901 gcgatgtggc tgatttcgtg agcaattacc gcctcggctt catccgggct catgttctgc
     6961 agcaaaccgg tgctgacagc aaccagagag gcatcacggc gcgcaccggt tgcaaaagcg
     7021 ttgatgtccg gcgcatggta gatagccact tgcggcatag cgatccccgc ctgacgagcc
     7081 tgggttgcta cagtattgac cagccaacgt tccctttcgt tacgcggttg ctcgatcact
     7141 tccccgccaa cagatcgtaa tgccatccat ttggacatca gaagcgaaac gaaggaacca
     7201 ccaaaaccga acagcaaggc catgatcatc agcccctgaa cgctgctcga ctgtatccct
     7261 gtcaggctca gtaccagccc gaaaacgacc attacggcca ggttcgttag caggaagagc
     7321 gcgattcgca tcataatttt ctttttacct cagtttaaca aaacgcaata tgcgataccc
     7381 acatcgtatg ggttacgcga ctattttcaa gtctggatag tgcgtaagtc accagaaaga
     7441 cacaacttta cattttgtag catctgattt acggcatctt gccgctgtta aaaaatcagg
     7501 cacaatttct tgtgcctgat tgatattact tgacgggagc gggttgttcc gcgggtctgg
     7561 ctttttcaag cttcgccaga tcgagtgcga tattcaccgt ctcatccaga taaggatccg
     7621 gctcctggta atctttcgat agatcatcca gcttcttcaa ctccggttta ccttctcgtt
     7681 taaagcgttc gttcaaacgc gccagacgcg tcgcatcatc ttcattattc tctttctcac
     7741 gcacagcgta gttcagagaa acgatattgc gcttgtcctt catagcgttg aagcgcgcga
     7801 tatccttcat gatgttctgg aactcaggat ctttcgcgat acgcgcatta tgttccttca
     7861 gcagctccgg ttcaaaggca gttaaatctc ctgatttcac ataagtcgcg gcatcgatgc
     7921 tatcccacgg cagcgcgtta tcttcgaatt tctcacccgt ttccgtttct tcattacccg
     7981 tcggcatgat gatgtctggt gttacgcctt tacgttgcgt actgccgccg ttaacgcgat
     8041 agaatttctg gatcgtgtac tgcacagaac ccagcgctgg ccattcagga cgtaacatct
     8101 gatcgtaaat acggttcaat gaacggtatt gctgaacggt gcctttacca aacgtcggtt
     8161 cacccacaac cagcgcacga ccgtaatcct gcattgccgc ggcaaagatt tctgaagccg
     8221 aagcactgaa acggtcaacc agtaccacca gcgggccttt atagaacacc tgcccgtcgg
     8281 tatcgctgtc ttcacgaacc ttgccgttgt tatcgcggac ctgaacaatg ggacccgcag
     8341 gaataaacag acctgagagc gataccgctt cggtcaacgc cccaccgcca ttgctacgca
     8401 ggtcgatgat cacactgctg acattctgtt tttccagttt ctgcagttga actttgacat
     8461 cgtctgtcaa acccacatag aagcccggaa tatccagcac gccgactttc tctttaccga
     8521 cggtcttcac cgacatttta accgcgcggt cttcgagacg aatacgttca cgggtcaacg
     8581 ttacagtacg ggtcttggtc cctttaccag caggtaaaat ttccagacga actttactgc
     8641 ccttcggccc tttaattaag gcaaccacat catcaagacg ccagccaatc acgtcaacca
     8701 tcggcttgcc tgtttgacca acaccgacaa ttttgtcacc aacgctgata gctttactct
     8761 tcgctgccgg accacctgcc accatcgaat tgataacggt gtagtcatca tccatttgca
     8821 gcactgcgcc aataccttcc agtgacaaac tcatttcagt gttgaactgt tcggtattac
     8881 gcggggaaag atagttggta tgcggatcga tttcacgcgc aaacgccgtc attgccagcg
     8941 agaaaacatc ttcgctgttg gtttgcgcca gacgacgaat ggcaaatttg tagcggcgtg
     9001 tcagggtttc acgaatttct ttatccgttt ttcctgtcag cttcaggctt aactcgtcga
     9061 atttgacttt actgtcccac tgcgcgttca actcagcctc gtttttcggc cagggcgctt
     9121 tgctgcggtc aaggttataa gtgtcgttgc cggtgaaatc catcggcttt tccagtaccg
     9181 acaaagcgta ctggtaacgt tcaaaacggc gcttttgcgc cagattgtag agatcgtaga
     9241 aaacgtcgag tttgcctgaa cgcagttcat cgcctaactc ggttttcttt ttcgcgaact
     9301 gttcaacatc gcttgccagc agcacattgt ggctgtaatc gagcagattc aggtagcggt
     9361 caaagatttt ggccgaaaat gcctgatcga ggtcgaactg gcgataatga gaacgggtga
     9421 agcgcgacgt tacgcgctca cttaccgtcg catgctgcgt ctcttccttt aataccggaa
     9481 tttgatcagc acgcgtgata tcttctacag cgaaggtctg gcctgctatt gcaagcaggc
     9541 cagctaacgc ggtaagccta aaaaacatgt tcatgcctgg cccggcctcc gtttcagaac
     9601 accaggtgtt ctgcgcgcac aatcaaagac atacccgaat tcagctggac gcggacgccg
     9661 tctttggtga tttctaatac ggtggcatcc atcgcgtttt gacccgcttt caccttcagg
     9721 gcttgtccga cagtcagagc tgaaatgtca gaaaccgggg tgtgctgttc ttcgcgaggt
     9781 gcttttactg tttttggcgc tttctctacc ggcttttgcg cacgaggttt acgttcagcg
     9841 ccttctttgc ggcgtggcgt ggtcggacgt ggcttgcgtt cgcggcgcgg tgcgtcttct
     9901 ttctcaccag cagctgcggc agcttcgcgt tttttcgctt gctgttcagc acgctgtgcc
     9961 tgaacacgcg ctttcgcttc ttcaagctgc ttgcgagcat gctctacatg ttgctcgtcc
    10021 agctcaccgc atgggttgcc gtcaagatcg acacgcgttg cgccgggttt aacaccgtaa
    10081 agataacgcc agctcgaagt gtagagacgt aaagcggatc gtaattgcgt tttgctcagg
    10141 ttcatttccc cggcaacacg atcgaccaaa tcctgaaaaa taccaatttt cagcggacgc
    10201 gcttcacctt ccgcactgaa acagtgggga aaacgttcgg ccagaaacgc gattacttct
    10261 ttactgctat tcaacttagg ttgattttcc atgaaatttc ctgattacaa cggacgtagc
    10321 caacaagcgc aggcatgaac aggcgtcatt ataatgacgc taccagtaaa tgctacgtta
    10381 tccgttgatt atcctgcgac gctcgcaaag aattttttgt aatccgtcgt tgcaagcact
    10441 ttttcaagct gtgccacaag ctgacgtaag ccttgctcgt cctcgtctgt aaagcgagcg
    10501 aagacagtac tatcgatgtc gagaacacca ataatctgat ttttcaccac cagcggcaga
    10561 acaatttcag aattactcgc cgcatcacag gcaatatgcc cgtcaaacgc gtgaacatcc
    10621 tcgatacgct gcacttgatt gcgggcaacc gctgtgccgc acaccccgcg cccgacgggt
    10681 atccggacac aggcaatttt gccctgaaat ggtccgagta ccagtgtatc gtcctcaagc
    10741 aaataaaaac ctgcccagtt aacgtcagtg agacgctcgt ataacaacgc actggtgttc
    10801 gcaagcgttg ccagaaaact ggtttctccc gccatcagcg cgttaaagtc gcgatttaaa
    10861 tccgcgtaaa attctgtttt gttcattact taatcacttg gttgtcttac ctggaactgc
    10921 cagcctatta aaataagcat taaatgcgtt aatgctcaag atcattccca tcatgggtta
    10981 agattaatgt taattcttat tacatttggc acgtcatggc tcttaacaca ccacaaatta
    11041 cgccgacaaa aaagataaca gtgagggcaa tcggcgagga actgccgcgt ggtgattacc
    11101 aacgttgccc gcaatgtgac atgctgttta gcctgcccga gataaattct catcaaagtg
    11161 cctattgtcc gcgctgtcag gcaaaaattc gcgacgggcg cgactggtcg ctaacgcgcc
    11221 tggcggcaat ggccttcacc atgctgttgc ttatgccgtt tgcctggggc gaaccgctgt
    11281 tgcatatctg gctgttaggc attcgtattg acgccaacgt catgcaaggc atctggcaaa
    11341 tgaccaaaca gggcgatgcg ataacgggat caatggtctt tttctgcgtt attggtgccc
    11401 ccctcattct ggtgtcctcc atagcatatt tatggtttgg taaccgactg ggaatgaatt
    11461 tacgtccggt actgctgatg cttgagcgac ttaaagagtg ggtaatgctg gatatctacc
    11521 tggtcggcat tggcgttgct tctataaagg tacaggatta tgcccatatc caggcgggtg
    11581 tcggcttgtt ctcttttgtg gcgttggtga ttttaacgac ggtgacgttg tcacatctta
    11641 atgttgagga gctgtgggag cgatattatc cgcagcgccc cgctacgcgt agggacgaga
    11701 aacttcgtgt ctgtcttggg tgccatttta ccggctatcc tgatcagcgt ggtctctgcc
    11761 cgcgttgcca tatcccgcta cgcctgcgtc gtcgtcatag tttgcaaaaa tgctgggcgg
    11821 cgctgttagc gtcaatcgtt ttgttgttac cagccaacct attgcctatt tctatcattt
    11881 atctgaatgg tggccggcag gaagatacga ttctttccgg aattatgtcg ctggcaagta
    11941 gcaacattgc ggttgcggga atcgtgttta tcgccagtat tctggtaccg tttactaaag
    12001 tgatcgtcat gttcacttta ctgttgagca ttcattttaa atgccagcaa ggtttacgca
    12061 cacgcattct gttactgcgg atggtgacct ggattggacg ctggtcgata ctcgacctgt
    12121 ttgtcatatc tttaaccatg tcgctgatta atcgcgatca gatcctcgct tttactatgg
    12181 gaccggctgc gttttatttt ggcgctgccg taattttgac tattcttgct gtggaatggc
    12241 tggacagccg cttactttgg gatgcacatg agtcaggaaa cgcccgcttc gacgactgaa
    12301 gcgcagatta aaaataaacg ccgtatctca cctttctggc tgctgccttt catcgcgcta
    12361 atgattgccg gttggctgat ttgggacagt tatcaggacc ggggtaatac cgtcaccatc
    12421 gactttatgt cggcggatgg tattgttccg ggccgtacgc ctgttcgtta tcagggcgtt
    12481 gaagtcggaa cagtgcagga tatcagcctc agcgacgatc ttcgtaagat tgaagtcaag
    12541 gtcagcatca agtccgatat gaaagatgcg ctgcgcgaag agactcagtt ctggctgatg
    12601 acgccaaaag catcgttggc aggtgtctcc gggctggacg ccctcgtcgg tgggaactat
    12661 atcggcatga tgccgggtaa aggtaaagag caggatcact ttgtcgcgct cgatacccaa
    12721 ccgaaatatc ggctggacaa tggcgatctg atgatccacc tgcaagcccc cgctctcggt
    12781 tcgctgaaca gcggttcatt ggtctatttc cgcaagatcc cggtgggaaa agtctacgac
    12841 tatgccatca atcccaacaa gcaaggcgtg gtgattgatg tcctgatcga gcggcgtttt
    12901 accgatctgg tgaaaaaagg tagccgtttc tgaaacgttt ccggcgttga tgccaacgtc
    12961 agtatcagtg gcgcgaaggt gaaactggaa agtctggcgg cactggttaa cggtgcgatt
    13021 gccttcgatt cacctgaaga gtcgaaacct gccgaggcgg aagatacctt tggtctgtat
    13081 gaagatctgg cccacagcca gcgtggagta ataataaaac tggaactgcc gagtggagca
    13141 ggattaaccg ccgactcgac gccgttaatg tatcaggggc tggaagtcgg acagctgact
    13201 aaactggatt taaatcctgg tggtaaagtc actggggaaa tgaccgttga tccctgcttc
    13261 gtgaaaatac ccgcatcgaa ttacgcaacc cgaaattatc ccttagcgat gctaatctca
    13321 gcgccctgct gaccggaaaa acctttgagt tggtgcccgg cgatggcgag ccacgcaaag
    13381 agttcgttgt tgtgccgggc gaaaaagccc tgctgcaaga gccggacgtt ctgacactga
    13441 ccctgacagc accggaaagt tacggtattg atgcgggtca gccgctcatt cttcacggcg
    13501 tgcaggtagg ccaggttatc gatcgtaaac tcaccagcaa aggcgtcacc tttaccgtcg
    13561 ccatcgagcc tcagcatcga gaactggtaa aaggcgatag caaatttgtc gtcaacagcc
    13621 gtgtcgacgt gaaggtgggg ctggatggcg ttgagtttct cggtgccagc gcctcagaat
    13681 ggattaacgg cgggatacgt attctgccgg gcgataaagg cgagatgaaa gccagctatc
    13741 cactgtatgc caatctggaa aaagcgctgg agaacagcct tagcgattta cccaccacaa
    13801 ccatgagttt gagtgcagag acgctgccgg atgtgcaggc aggatcggta gtgctgtacc
    13861 gtaaatttga agttggtgaa gttattaccg tgcgtccgcg agctaacgcg ttcgatatcg
    13921 atctgcacat taaaccggaa tatcgcaacc ttttgaccag caacagcgtg ttctgggcag
    13981 aaggcggggc gaaagttcag ctgaatggta gtggcctgac cgtacaggca tccccgctct
    14041 ccagagcatt aaagggggcc attagcttcg ataacctcag tggtgccagc gccagtcagc
    14101 gtaaaggcga caagcgaatt ctgtatgctt ccgaaacagc ggcccgtgcg gttggcaggc
    14161 agattacgct tcacgctttc gatgccggaa aactggcggt cgggatgcca attcgctatc
    14221 tcggtattga tatcgggcaa atccagacgc tggatctgat taccgcacgc aatgaagtac
    14281 aggcaaaggc ggtgctctat ccggaatatg tccagacctt tgctcgcggt ggtacgcgct
    14341 tctcagtggt cacaccgcaa atctcggcag ctggcgttga gcatctagat actatcctcc
    14401 agccgtatat caacgtcgaa ccaggccgtg gcaatcctcg tcgcgacttt gaattagaag
    14461 aggccaccat tactgattcg cgttacctag atggtttaag cattattgtt gaagcgccgg
    14521 aagccggttc gttaggcatc ggtacgcctg tgctgttccg tggtctggaa gtaggtacgg
    14581 ttaccggaat gacgctgggg acattgtcag atcgcgtgat gattgcgatg cgcatcagta
    14641 aacgctatca acacctggtg cgtaacaatt ccgtcttctg gttggcatcg ggttacagtc
    14701 tggactttgg tctgacgggc ggcgtagtga aaaccggcac ctttaaccaa tttatccgtg
    14761 gcggcatcgc cttcgccacg cctccgggta cgccacttgc accgaaagcc caagaaggca
    14821 aacacttcct gttgcaggaa agtgaaccga aagagtggcg tgaatgggga actgcgcttc
    14881 ccaaataatg cccactgctc cggcgtgcct gcgccggagc gcttatgcta aactgcgcgc
    14941 ctgttttttt gccagtggta catgctcgtg gcccaacaca ccgtttattt cccggacgcg
    15001 tttctgacac aaatgcgcga agctatgcct tcgacgctct catttgatga ttttcttgcc
    15061 gcctgtcagc gcccgttgcg ccgcagcatt cgcgttaata cgctgaaaat ctccgttgct
    15121 gatttcctgc aattaaccgc tccttatggc tggacgctta cgccaattcc gtggtgtgaa
    15181 gaaggtttct ggattgaacg cgacaatgaa gatgcattgc cattgggtag taccaccgag
    15241 catttaagtg gcctgtttta tattcaggaa gccagttcaa tgttgcccgt cgccgccttg
    15301 tttgctgacg gtaatgcacc acagcgggtg atggatgtcg ctgctgcgcc aggctcaaaa
    15361 acgacgcaaa ttgccgcgcg gatgaataac gaaggggcaa tccttgccaa tgagttttcc
    15421 gccagttggg taaaagtgtt acatgccaat atcagccgct gtggcatcag taatgttgcg
    15481 ctcacacatt ttggtggccg cgtgtttggt gcggcagtgc cagaaatgtt tgatgccatt
    15541 ttgctggacg ctccctgctc cggcgaaggc gtggtgcgta aagatcccga tgcgctaaaa
    15601 aactggtcac ccgaaagcaa tcaggaaatc gcagctaccc aacgggaact gattgacagc
    15661 gcctttcatg cattacgtcc tggcggtacg ctggtttact cgacctatac cttaaaccgg
    15721 gaagaaaacg aagccgtttg ccggtggctg aaagagactt accccgacga agtagagttt
    15781 ttaccacttg gcgatctctt ccctggtgca aacaaggcgc tgaccgaaga aggttttttg
    15841 catgttttcc cacaaattta cgactgcgaa ggcttcttcg ttgctcgtct gcgtaaaact
    15901 caggcgatcc ccgccttacc cgccccaaaa tacaaagtgg gcaatttccc gttcagcccg
    15961 gtgaaagatc gcgaagccgg acaaattcgt caggcggctg caagtgttgg cttaaactgg
    16021 gatggaaacc tgcgactctg gcaacgcgac aaagaactgt ggttgttccc ggtaggcatt
    16081 gaagccctga tcggtaaagt ccgattttct cgcttaggga ttaaacttgc cgaaacgcat
    16141 aacaaaggtt atcgctggca gcatgaagcg gttattgccc atgcctcccc cgacaatgta
    16201 aacgcttttg aactgacacc gcaggaagcg taggagtggt atcgcgggcg cgatgtttac
    16261 ccgcaagccg cgccagtggc ggatgacgtg ttggttactt tccagcatca gccgattggt
    16321 ttagccaaac ggattggttc gcgactgaaa aacagctacc cgcgtgaact ggtgcgggac
    16381 gggaaacttt ttaccagtaa cgcatgacag cgcacaaaaa aagcgcactt tttgactggc
    16441 acattcgccg gcctcaacta ggctgaaaaa ttgtgcgatc ggactggtcg tactacaatc
    16501 agcagctaaa tggagagcac gaagatgaaa accagtgtgc gcataggcgc ttttgaaatc
    16561 gacgacggcg aattacacgg tgaatcgccg ggtgatcgaa cgttaaccat tccttgtaaa
    16621 tctgaccccg atttatgtat gcaactggat gcctgggatg ctgaaaccag tatccctgcc
    16681 ctgctaaatg gcgaacactc tgtcctttac cgtacccgtt acgatcaaca atctgatgcc
    16741 tggattatgc gtcttgcctg atccaaaaag aacccgtcgg catggcgggt tatttgtcct
    16801 ggttattccc ccgttgtaaa atctctccta aacttaacgg tacggcacca cacttcgggg
    16861 atgaaatgtt cgcgctggta ctttttgttt gctacctgga tggcggttgt gaagatattg
    16921 ttgtggatgt ctacaacacg gaacagcagt gtctttattc tatgagcgat caacggatcc
    16981 gccatggcag ttgttttccg attgaggatt ttatagatgg tttctggcaa ccagcacagg
    17041 agtatggtga tttttaatta ttgcagttgc acaagagtca gttagcc
//
LOCUS       DAJTRH010000081        16393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTRH010000081 DAJTRH010000000
VERSION     DAJTRH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO339_002917"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO339_002917"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5927475.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO339_002918"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO339_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5927476.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OO339_002919"
     CDS             2339..2554
                     /locus_tag="OO339_002919"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927477.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OO339_002920"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OO339_002920"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5927478.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4453..5847
                     /gene="galP"
                     /locus_tag="OO339_002921"
     CDS             4453..5847
                     /gene="galP"
                     /locus_tag="OO339_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5927479.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5924..6421
                     /locus_tag="OO339_002922"
     CDS             5924..6421
                     /locus_tag="OO339_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5927480.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6516..7223
                     /gene="endA"
                     /locus_tag="OO339_002923"
     CDS             6516..7223
                     /gene="endA"
                     /locus_tag="OO339_002923"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5927481.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7303..8034
                     /gene="rsmE"
                     /locus_tag="OO339_002924"
     CDS             7303..8034
                     /gene="rsmE"
                     /locus_tag="OO339_002924"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5927482.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8047..8997
                     /gene="gshB"
                     /locus_tag="OO339_002925"
     CDS             8047..8997
                     /gene="gshB"
                     /locus_tag="OO339_002925"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5927483.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9106..9669
                     /locus_tag="OO339_002926"
     CDS             9106..9669
                     /locus_tag="OO339_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5927484.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9669..10085
                     /gene="ruvX"
                     /locus_tag="OO339_002927"
     CDS             9669..10085
                     /gene="ruvX"
                     /locus_tag="OO339_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5927485.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10261..11241)
                     /locus_tag="OO339_002928"
     CDS             complement(10261..11241)
                     /locus_tag="OO339_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5927486.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11259..11963
                     /gene="yggS"
                     /locus_tag="OO339_002929"
     CDS             11259..11963
                     /gene="yggS"
                     /locus_tag="OO339_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5927487.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11981..12547
                     /gene="yggT"
                     /locus_tag="OO339_002930"
     CDS             11981..12547
                     /gene="yggT"
                     /locus_tag="OO339_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5927488.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12544..12834
                     /locus_tag="OO339_002931"
     CDS             12544..12834
                     /locus_tag="OO339_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5927489.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12842..13435
                     /locus_tag="OO339_002932"
     CDS             12842..13435
                     /locus_tag="OO339_002932"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5927490.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13428..14564
                     /gene="hemW"
                     /locus_tag="OO339_002933"
     CDS             13428..14564
                     /gene="hemW"
                     /locus_tag="OO339_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5927491.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14878..15841
                     /locus_tag="OO339_002934"
                     /pseudo
     CDS             14878..15841
                     /locus_tag="OO339_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15886..16389
                     /locus_tag="OO339_002935"
     CDS             15886..16389
                     /locus_tag="OO339_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5927492.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4060 a         4222 c         4124 g         3987 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttac atcattgcag atgacagcgt ttaatctatg atgatataac tcaattattt
     4321 tcatgccctt aaatcataac taagataaat gttagtgtaa gcgattacac tgatgtgatt
     4381 tgcttcacat ctttttacgt cgtactcacc tatcttaatt cacaataaaa aataactata
     4441 ttggagggca tcatgcctga cgctaaaaaa caggggcggt caaacaaggc aatgacgttt
     4501 ttcgtctgct tccttgccgc tctggcggga ttactctttg gcctggatat cggtgtaatt
     4561 gctggcgcac tgccgtttat tgcagatgaa ttccagataa cttcgcacac gcaagaatgg
     4621 gtcgtaagct ccatgatgtt cggtgcggca gtcggtgcgg tgggcatcgg ctggctctcc
     4681 tttaaactcg ggcgcaaaaa gagcctgatg atcggcgcaa tcctgtttgt tgccggttca
     4741 ctgttctctg cggctgcgcc aaacgttgaa gtactgattc tttcgcgcgt tctgctgggg
     4801 ctggcggtgg gtgtggcctc ttataccgca ccactgtacc tgtcagaaat tgcgccggaa
     4861 aaaattcgcg gcagtatgat ctcgatgtat cagttgatga tcactatcgg gatcctcggt
     4921 gcttatcttt ctgataccgc cttcagctac accggtgcat ggcgctggat gctgggtgtg
     4981 attatcatcc cggcaatttt gctgctgatt ggtgtcttct tcctgccaga cagcccacgt
     5041 tggtttgccg ccaaacgccg ttttgttgat gccgaacgcg tgctgctacg cctgcgtgac
     5101 accagcgcgg aagcgaaacg cgaactggat gaaatccgtg aaagtttgca ggttaaacag
     5161 agtggctggg cgctgtttaa agagaatagc aacttccgcc gcgcggtgtt ccttggcgta
     5221 ctgttacagg taatgcagca attcaccggg atgaacgtca tcatgtatta cgcgccgaaa
     5281 atcttcgaac tggcgggtta taccaacacc accgagcaaa tgtgggggac agtgattgtc
     5341 ggcctgacca acgtacttgc cacctttatc gcaatcggcc ttgttgaccg ctggggacgt
     5401 aaaccaacgc taacgctggg cttcctggtg atggctgctg gcatgggcgt actcggtaca
     5461 atgatgcata tcggtatcca ctctccgtcg gcgcagtatt tcgccatcgc catgctgctg
     5521 atgtttattg tcggttttgc catgagtgcc ggtccgctga tttgggtact gtgctccgaa
     5581 attcagccgc tgaaaggccg cgattttggc atcacctgct ccaccgccac caactggatt
     5641 gccaacatga tcgttggcgc aacgttcctg accatgctca acacgctggg taacgccaac
     5701 accttctggg tgtatgcggc tctgaacgta ctgtttatcc tgctgacatt gtggctggta
     5761 ccggaaacca aacacgtttc gctggaacat attgaacgta atctgatgaa aggtcgtaaa
     5821 ctgcgcgaaa tcggcgctca cgattaatct cctcaggctt cctcccgtcg cggaggaagc
     5881 cgcctcttgc agtcatcttt tcttcgctct atcctctgcc gctatgaaaa catcccgtct
     5941 ccctatcgcc atccaacagg ccgttatgcg ccgcctgcgg gaaaaactcg cccaggccaa
     6001 cctgaagcta gggcgtaact acccggagcc aaaactctct tacacccagc gcggaacctc
     6061 cgccggaaca gcctggctgg aaagctatga aatccgcctc aatcccgttt tgctgttgga
     6121 aaacagtgaa gcttttattg aagaagtggt accgcacgaa ctggcacatt tgctggtatg
     6181 gaaacatttc ggccgcgtag cgccacatgg caaagagtgg aagtggatga tggaaagcgt
     6241 gctgggcgtt cccgcccgtc gtacgcatca gttcgaactg caatccgtgc gtcgcaacac
     6301 cttcccctac cgctgtaagt gccaggaaca tcagcttacc gtacgccgcc ataatcgcgt
     6361 agttcgtggc gaggccatct atcgctgtgt tcactgcggt gaacagctgg ttgcgaaata
     6421 accacctgaa ctatcaggaa ctttcctgat ctggctgatt gcataccaaa acaactttcg
     6481 ctacgttgct ggctcgtttt aacacggagt aagtgatgta ccgttatttg tctattgctg
     6541 cggtggtact gagcgcagca ttttccggcc cgacgttggc cgaaggtatc aatagttttt
     6601 ctcaggcgaa agccgcggcg gtaaaagtcc acgctgatgc gcccggtacg ttttattgcg
     6661 gatgtaaaat taactggcag ggcaaaaaag gcgttgttga tctgcaatcg tgcggctatc
     6721 aggtgcgcaa aaatgaaaac cgcgccagcc gcgtagagtg ggaacacgtc gttcccgcct
     6781 ggcaattcgg tcaccagcgc cagtgctggc aggacggtgg acgtaaaaac tgcgctaaag
     6841 atccggtcta tcgcaagatg gaaagcgata tgcataacct gcagccgtca gtcggcgagg
     6901 tgaatggcga tcgcggcaat tttatgtaca gccagtggaa tggcggcgaa ggccagtacg
     6961 gtcaatgcgc catgaaggtc gatttcaaag aaaaagctgc cgaaccacca gcgcgtgcac
     7021 gcggtgccat tgcgcgcacc tacttctata tgcgcgacca atacaacctg acactctctc
     7081 gccagcaaac gcagctgttc aacgcatgga acaagatgta tccggttacc gactgggagt
     7141 gcgagcgcga tgaacgcatc gcgaaggtgc agggcaatca taacccgtat gtgcaacgcg
     7201 cttgccaggc gcgaaagagc taacctacac tagcgggatt ctttttgtta acccctaccc
     7261 cacgcgtaca accgcgtggg gagacgacgc ggatttttaa ctatgcgtat cccccgcatt
     7321 tatcatcctg aaccactgac cagccattct cacatcgcgc tttgcgaaga tgccgccaac
     7381 catatcgggc gcgtactgcg catggggccg gggcaggcgt tgcaattgtt tgacggtagc
     7441 aaccaggtct ttgacgccga aattaccagc gccagcaaaa aaagcgtgga agtgaaggtg
     7501 ctggaaggcc agatcgacga tcgcgaatct ccgctgcata ttcacctcgg tcaggtgatg
     7561 tcgcgtggtg aaaaaatgga atttactatc cagaaatcga tcgaactcgg tgtaagcctc
     7621 attacgccac ttttttctga gcgctgcggc gttaaactgg atagtgaacg tctgaacaag
     7681 aagcttcagc agtggcagaa gattgcaatt gctgcctgtg agcagtgtgg tcgtaaccgg
     7741 gtgccggaaa tccgtccagc gatggatctg gaagcctggt gtgcagagca ggatgaagga
     7801 ctgaaactga atcttcaccc gcgcgccagt aacagcatca atacgttgcc tttaccggtt
     7861 gaacgcgtcc gcctgctgat tggcccggaa ggcggtttat cggcagatga aattgccatg
     7921 actgcccgct atcaatttac tgatatcctg ttgggacctc gcgttttgcg tacagagaca
     7981 actgcgctca ccgccattac cgcgctacaa gtacgatttg gcgatttggg ctaacggaga
     8041 agaataatga tcaagctcgg catcgtgatg gaccccatcg caaacatcaa catcaagaaa
     8101 gattccagtt tcgctatgtt gctggaagca cagcgtcgtg gttacgaact tcactatatg
     8161 gagatgggcg atctgtatct gatcaatggc gaagcccgcg cccatacccg cacgctgaac
     8221 gtgaagcaga actacgaaga gtggttttcg ttcgtcggtg aacaggatct gccgctggcc
     8281 gatctcgatg tgatcctgat gcgtaaagac ccgccgtttg ataccgagtt tatctacgcg
     8341 acctatattc tggaacgtgc cgaagagaaa gggacgctga tcgttaacaa gccgcagagc
     8401 ctgcgcgact gtaacgagaa actgtttacc gcctggttct ctgacttaac gccagaaacg
     8461 ctggttacgc gcaataaagc gcagctaaaa gcgttctggg agaaacacag cgacatcatt
     8521 cttaagccgc tggacggtat gggcggcgcg tcgattttcc gcgtgaaaga aggcgatcca
     8581 aacctcggcg tgattgccga aaccctgact gagcatggca cttgctactg catggcgcaa
     8641 aattacctgc cagccattaa agatggcgac aaacgcgtgc tggtggtgga tggcgagccg
     8701 gtaccgtact gcctggcgcg tattccgcag gggggcgaaa cccgtggcaa tctggctgcc
     8761 ggtggtcgcg gtgaacctcg tccgctgacg gaaagtgact ggaaaatcgc ccgtcagatc
     8821 gggccgacgc tgaaagaaaa agggctgatt tttgttggtc tggatatcat cggcgaccgt
     8881 ctgactgaaa ttaacgtcac cagcccaacc tgtattcgtg agattgaagc agagtttccg
     8941 gtgtcgatca ccggaatgtt aatggatgcc atcgaagcac gtttacagca gcagtaaccc
     9001 accttagcga gaaggatctc gttgagactc tgagtgacag cgcccttctt tccacgcata
     9061 ctgggcgctg ttgctttttt gaaccaggaa acagaacctc tgacaatgaa tttacagcat
     9121 cactttctta ttgccatgcc tgctctccag gatccgattt tccgtcgttc cgtggtctac
     9181 atttgcgaac ataataccaa tggtgcaatg gggatcatcg tcaacaagcc gctggaaaat
     9241 ctcaaaattg aagggattct ggaaaagctg aagatcacgc cggagccgcg tgatgaatca
     9301 atccgtctgg ataaaccggt tatgctcggc ggcccactgg ctgaagatcg cgggtttatt
     9361 ttgcatactc cgccctccaa ttttgcttcc agcattcgca tttcagacaa cacggtgatg
     9421 accacttccc gcgatgtgct ggaaacgctc ggcaccgata aacaaccgtc tgacgtattg
     9481 gtggctctgg gctatgcctc ctgggaaaaa ggtcaactgg aacaagaaat tctcgataac
     9541 gcgtggctaa cggccccggc ggatctgaat attctgttca aaacgccgat tgccgaccgc
     9601 tggcgcgagg cggcaaaact gattggtgtg gatattctca ccatgcctgg tgtggcagga
     9661 cacgcctgat gagtggaacc ttactcgcct tcgacttcgg caccaaaagc attggcgtag
     9721 cggtcggcca acgcattacc ggcaccgctc gccctttgcc tgcaattaaa gcgcaggacg
     9781 gtacgccgga ctggaacctc atcgaacgtt tactgaaaga gtggcagcct gacgaaatca
     9841 tcgtcggttt gccgctgaat atggacggca ccgaacagcc gttaaccgcc agagcgcgca
     9901 aatttgccaa ccgtattcat ggtcgtttcg gtgttgaagt aaagctccat gacgagcgtc
     9961 ttagcactgt ggaagcccgt tccggtcttt ttgaacaggg cggctatcgg gcgctcaaca
    10021 aaggcaaagt tgactctgcc tctgcggtta ttattctcga aagctatttc gagcagggat
    10081 attaaggcga tctaaaagcc tggcggatag tcgttaccgc catcttgccg gatgcggcgt
    10141 gaacgcctta tccagcacac atctggcagc ggctgtaggt ctgataagac gcgttagcgt
    10201 cgcatcagac atttattgcc tttataggcc ctataaactt gcgcatcggg catggcaacg
    10261 tcacaaacgc ccttccccca cccgctgctg ataactctgc tgaaacgtta acatccccac
    10321 cttctgcccg gtttgaataa catgcggtaa ctggtgggtt ttcccttcgc gaatcaaatt
    10381 ccccaccgcg ggagtgttaa tcagtaattc aaacagcgcc acgcgtcctt cctgtttatc
    10441 cacttccagc ttttgtgaca gcactgcccg taaactaccc gcaagttgat tacgtacggg
    10501 atctttttcc tgcgccggaa atgaatccac cagtcgctca actgcctgcg cggcaccacg
    10561 tgtatgtaag gttgccagca ccagatgccc ggtttctgcc gccgtcagcg ccagacggat
    10621 tgtctcactg tcacgtagct ctccgagcaa aatcacatca ggatcttccc gcaatgcagc
    10681 ccgcaacccc gatgcgaacg tcatacagtg caaaccaatt tcccgctgct ggatcaaaca
    10741 tcgctggctg gcatagagat attccacagg atcttccagc gtcagaatat gcgcatcggc
    10801 atgttgatta agatagccaa ccatcgccgc cagcgtggta gatttgccac tccccgtagc
    10861 ccccgtcacc agaatcaggc cattctcgct cttgagtaat tccggcaata ccggtggtgc
    10921 accaagctgt tcgagctgtg gacagtgcga aggtaacaac cgtaatgcca gagaaatacc
    10981 ttgccgttgc gcgaacgcac tgccacgcaa ccgctggttt tccgccagcg acacggcaaa
    11041 atccagctgg ccattttcca gcaatattgc ccgctgatcg tcatccagcc actcccgcag
    11101 tagctcttcg acgtccggcg catcaaacgg cgcagcttcc attctgccac gaatgcgcca
    11161 tcgtgcgggc caggcgctgc acaggtgtag atccgagacg ttatgcttta cactaagggc
    11221 cacaatttct tccatattca tactaagatc ctcggaaaat gaacgatatt gcgcataacc
    11281 tggcacaggt ccgggacaaa atctcagcgg ctgcaacgcg ttgcggccgt tctccagaag
    11341 aaattacgct gcttgcagtc agtaaaacaa aacctgcgag cgccatcgca gaagccattg
    11401 atgccgggca gcgtcaattt ggtgaaaact acgttcagga aggggtagat aaaattcgcc
    11461 actttcagga actgggcgta acaggattag aatggcattt tattggcccg ttgcagtcta
    11521 ataaaagccg cctagtggca gagcatttcg actggtgtca taccatcgac cgtttgcgca
    11581 ttgctacccg cctcaacgag cagcgcccgg cagaacttcc ccctcttaac gtactgattc
    11641 aaattaatat tagtgatgaa aacagtaagt ccgggattca actggcagaa ctggacgagc
    11701 tggcagcggc ggtcgctgaa ctaccgcgtt tacgtctgcg cgggctgatg gcaatccctg
    11761 cgcttgagtc agaatatgta aggcagtttg aagttgcacg ccaaatggct gtagcatttg
    11821 ccggactgaa aacgcgctac ccgcatatcg acacgctctc tctgggaatg tcggacgata
    11881 tggaagccgc cattgcggca ggtagcacga tggttcgtat cggcactgca atttttggtg
    11941 cgcgtgatta ctctaaaaaa taaggaatta aaggaacgcc atgaatacgt tgactttcct
    12001 gctttcaacg gtcattgagc tgtatacaat ggtgctgtta ttacgcatct ggatgcagtg
    12061 tgctcattgt gatttttaca cccccttctc acagtttgta gtgaaggtag cgcagccaat
    12121 tatcgggcca ctgcgccgcg ttattccggc aatgggtccc attgacagcg cctcgctgct
    12181 ggttgcctat attctcagtt ttatcaaagc cattgtgctg tttgaagtgg tgaccttcct
    12241 gccaatcatc tggattgccg gtttactgat tctgctgaaa accatcggct tgctgatttt
    12301 ctgggtcctg ctggtgatgg cgattatgag ctgggtaagc cagggtcgta gcccgattga
    12361 atacgtgctg attcagctgg ccgatccgct gctgcgcccg attcgccgct tgctaccggc
    12421 aatgggtggg attgatttct cgccgatgat cctcgttctg ctgctgtatg ccatcaatat
    12481 gggtgtcgca gaagtattac aggcaaccgg aaacatgctg ctgccggggc tgtggatggc
    12541 gttatgagtg ccgtaacagt taatgatgac ggtctggttt tacggctcta tattcagccg
    12601 aaagccagcc gtgattctat tgtcggttta catggcgacg aagttaaagt cgccattacc
    12661 gcgccgccgg ttgacggcca ggccaacagt catctggtga agtttctcgg taagcaattc
    12721 cgggttgcca aaagccaggt ggtgattgaa aaaggcgaac ttggccgcca caaacaaatt
    12781 aaaatcatta atccgcaaca aatcccgcca gaaatcgcgg cgttaattaa ttaggtatcc
    12841 tatgcaaaaa gttgtcctcg caaccggcaa tgccggtaaa gtgcgtgagc tggcgtcgct
    12901 gcttagcgac tttggtcttg atatcgtggc ccaaacagac ctcggcgttg attccgctga
    12961 agaaaccggc ctaaccttta tcgaaaacgc gattctgaaa gcgcgccatg cggcaaaagt
    13021 gaccggttta ccggcaattg ccgacgactc tggtctggcg gtagatgcgc tgggtggcgc
    13081 gccgggtatt tactccgcgc gttattccgg tgaagacgcg accgatcaaa agaatctgca
    13141 aaaactgttg gaaacactga aagacgtacc ggacgaccaa cgtcaggcgc gtttccactg
    13201 cgtgctggta tatctgcgtc acgcggaaga tccgactccg ctggtatgcc acggcagctg
    13261 gccgggcgtg attactcgcg aaccagcggg cactggtggc tttggttatg atccaatctt
    13321 cttcgtacct tccgaaggga aaaccgccgc cgaactgacc cgcgaagaaa agagcgccat
    13381 ttcccaccgt ggtcaggcgt tgaaactgct gctggacgct ttacgtaatg gttaaattac
    13441 cgccgctgag tctctacatt cacatcccgt ggtgcgtgca gaaatgcccg tactgcgatt
    13501 tcaactctca cgcgttgaaa ggagaagtgc cgcacgacga ttatgttcag catctgcttt
    13561 gcgatctgga caatgatgtg gcttacgctc agggccgtga agtaaagaca atctttattg
    13621 gcggtggtac gccgagcctg ctttccggcc cggcgatgca aacgctgcta gacggcgtgc
    13681 gtgcgcgttt gccgctggca gcggatgcag aaattactat ggaagcgaac ccaggcacgg
    13741 tagaagcgga tcgctttgtc gattatcagc gtgctggtgt aaaccgcatc tctattggtg
    13801 tgcagagttt tagcgaagaa aagctgaaac gacttgggcg tattcatggc ccgcaagaag
    13861 cgaaacgagc ggcaaagctg gcgagcggtt tagggttacg tagctttaac cttgatttga
    13921 tgcatggact accggatcaa tcactggaag aggcgcttgg cgatctacgc caggccattg
    13981 aactgaatcc gccgcatctt tcctggtatc aactgactat cgaacctaat acgctgtttg
    14041 gctcgcgacc gccggtgctg ccggacgatg acgcgctgtg ggatattttc gaacaggggc
    14101 atcagttatt aaccgcagcg ggttatcagc aatatgaaac ttccgcttac gccaaacccg
    14161 gttatcagtg ccagcacaat ctcaactact ggcgctttgg tgactacatc ggtattggct
    14221 gcggcgcgca cggcaaagtg accttcccgg atgggcgcat tctgcgtacc actaaaacgc
    14281 gtcatcctcg tggttttatg caaggaaggt atctggaaag ccagcgtgat gtcgaagccg
    14341 cagataagcc gtttgagttc tttatgaatc gcttccgtct gctggaggcc gcgccgcgcg
    14401 tggagtttat tgcgtatacc gggctttgcg aagatgtgat tcgcccacag ttagacgagg
    14461 cgattgccca gggttatctc accgaatgtg cggattactg gcagataacg gaacatggta
    14521 aattattctt aaactcactt cttgaattat tcctcgctga gtaatcatta atgcgcctgc
    14581 gggcgcatta atcacttctt ttatcccccc gttaattaac ctcggcagaa atatatttct
    14641 tactgaataa atatcgacga catttattat atatatttag tgaatattta ctaatcattc
    14701 attgattata aatatgtaca acctggcgca caaatttaat atttaatttc tgactttatt
    14761 tcaagttgtg ataaacatag acaagttgtc agacaggtta accaatttgt cgcacaaatc
    14821 actgataagc acaattagat atcaaccaat ttccatcctt caacagggag tttttttatg
    14881 aaaatgaggt catttacccg ctcattagtc tgcgcatcct tgctggctct ggttagcact
    14941 ggcgtaaacg ccgcagaaaa agtggcgtta aagttagcac acaacctgga aagaagtcat
    15001 gttgtacatc agagttttga agagctggcg aaagaggtaa aacagctgtc aaaaggtaat
    15061 atggtgatcc gtatttatcc cagcagccag atgggtaatg cgcgagaaac tatggaattg
    15121 ttgcaaaatg gcgcactgga tatgaccaaa ggttccgcca gtgatctgga gtcttttgat
    15181 aatatttatg ctatctataa tttgccgttc ttattaagtt gtttttggtg aagttggtaa
    15241 agaaattatg gactcaacaa aagacaaagg cttttttgct ttatcagctt atgtagcggg
    15301 tactcgcagt ttctatgcga aaaaaccgat tacaaagcca gaagatctta aaggactgaa
    15361 aattcgtgtt cagccaagtc cgaccaccat caaaatgatt gaactgatgg gtggttcacc
    15421 cactccaatt tcatttggcg aagtttatac tgcgatgcag caaggtgtag tagatggcgc
    15481 agaaaataac gtgccttcct ggatgcagac tcgacatatt gaaattgcca aagttttttc
    15541 tgaagatgaa cacgcctcaa ttcctgactt tttggttatc tccaccaaaa catggaataa
    15601 attaacccca gaacagcaac aaatcctcga aacagcggcg aagaaatccg aagcttatca
    15661 acaaaaattg tgggaaaaaa ttgacgctga cacacgtgct caggcgaaag ctatgggggg
    15721 tgaaattgta aaagtggata aagcgccatt ccgtaaagcc gtgcaacctc tgtttgacga
    15781 cttcaaaaaa gatccaaaac aagctgcttt gttagagaaa ttcgacaacg ctgctcaata
    15841 atattccggg ccagcctctg gccctgattt tccaggatgc ttacaatgat atttaaccgc
    15901 ctgaaacttg cggtagaccg cgtcattgcc acgttttctg tcgccgtaat gcttgctctg
    15961 gttgtatgcg tagtctggca agtatttagc cgctacgtac ttaaccagcc aagtacatta
    16021 accgatgagc tggcacgctt tttgatgatc tgggtaggat tattaggtgc tgcatatacc
    16081 gtcggcgcgc agcgacatct ctctattgat ttatttgctc tcgccctgaa taagcgcaaa
    16141 caattacttc ttagtatagt cattaacgtt ctcattttag gttttgccgg ttcggtcatt
    16201 gtgactggtg gcctgaaatt aattgataaa acactggcaa catcacaggt atctgccgct
    16261 atgcaaatac cgatgggata cgtatatatc attctgccat taagtggagt ggtaatgatg
    16321 ttttatgcct tgtgttttat taatcaaagc attcaacaac tgaaacaacc tgcgcaggag
    16381 gccagttaat gga
//
LOCUS       DAJTRH010000082        16386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTRH010000082 DAJTRH010000000
VERSION     DAJTRH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            21..2483
                     /locus_tag="OO339_002936"
     CDS             21..2483
                     /locus_tag="OO339_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5927493.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            2499..3532
                     /locus_tag="OO339_002937"
                     /pseudo
     CDS             2499..3532
                     /locus_tag="OO339_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(3614..3952)
                     /gene="rcnB"
                     /locus_tag="OO339_002938"
     CDS             complement(3614..3952)
                     /gene="rcnB"
                     /locus_tag="OO339_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5927494.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(4171..5001)
                     /gene="rcnA"
                     /locus_tag="OO339_002939"
                     /pseudo
     CDS             complement(4171..5001)
                     /gene="rcnA"
                     /locus_tag="OO339_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            5123..5395
                     /gene="rcnR"
                     /locus_tag="OO339_002940"
     CDS             5123..5395
                     /gene="rcnR"
                     /locus_tag="OO339_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5927495.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5487..5583
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5618..6406
                     /gene="thiM"
                     /locus_tag="OO339_002941"
     CDS             5618..6406
                     /gene="thiM"
                     /locus_tag="OO339_002941"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5927496.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            6403..7203
                     /gene="thiD"
                     /locus_tag="OO339_002942"
     CDS             6403..7203
                     /gene="thiD"
                     /locus_tag="OO339_002942"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5927497.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            7268..8089
                     /locus_tag="OO339_002943"
     CDS             7268..8089
                     /locus_tag="OO339_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5927498.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            8137..8883
                     /locus_tag="OO339_002944"
     CDS             8137..8883
                     /locus_tag="OO339_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5927499.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8857..9822)
                     /locus_tag="OO339_002945"
     CDS             complement(8857..9822)
                     /locus_tag="OO339_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5927500.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9819..10822)
                     /locus_tag="OO339_002946"
                     /pseudo
     CDS             complement(9819..10822)
                     /locus_tag="OO339_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            10862..11914
                     /gene="fbaB"
                     /locus_tag="OO339_002947"
     CDS             10862..11914
                     /gene="fbaB"
                     /locus_tag="OO339_002947"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5927501.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            12222..13076
                     /gene="gatY"
                     /locus_tag="OO339_002948"
     CDS             12222..13076
                     /gene="gatY"
                     /locus_tag="OO339_002948"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5927502.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            13105..14367
                     /gene="gatZ"
                     /locus_tag="OO339_002949"
     CDS             13105..14367
                     /gene="gatZ"
                     /locus_tag="OO339_002949"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5927503.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            14377..14829
                     /gene="gatA"
                     /locus_tag="OO339_002950"
     CDS             14377..14829
                     /gene="gatA"
                     /locus_tag="OO339_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5927504.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            14860..15144
                     /gene="gatB"
                     /locus_tag="OO339_002951"
     CDS             14860..15144
                     /gene="gatB"
                     /locus_tag="OO339_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5927505.1"
                     /translation="MKRKIIVACGGAVATSAMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            15148..>16386
                     /locus_tag="OO339_002952"
     CDS             15148..>16386
                     /locus_tag="OO339_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5927506.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFS"
BASE COUNT         4178 a         3819 c         4240 g         4149 t
ORIGIN      
        1 tttaattgca ggagctgcct atgttgagaa tgaccccact tgcatcagca atcgtagcgt
       61 tattgctcgg cattgaagct tatgcagctg aagaaacctt tgatacccat tttatgatag
      121 gtggaatgaa agaccagcag gttgcaaata ttcgtcttga tgataatcaa cccttaccgg
      181 ggcagtatga catcgatatt tatgtcaata agcaatggcg cgggaaatat gagattattg
      241 ttaaagacaa cccgcaagaa acatgtttat caagagaagt tatcaagcgg ttaggcatta
      301 atagcgataa cttcgccagc ggtaagcaat gtttaacatt tgagcaactt gttcagggtg
      361 ggagctatag ctgggatatc ggggtttttc gtctcgattt cagtgtcccg caggcttggg
      421 tggaagaact ggaaagtggc tatgttccac cggaaaactg ggagcggggt attaatgcgt
      481 tttatacttc ttattatgtg agtcagtatt acagcgacta taaagcgtcg ggtaacaaca
      541 agagtacata tgtacgtttt aacagcgggt taaatttact ggagtggcaa ctgcattctg
      601 atgccagttt cagtaaaaca aataacaatc caggggtgtg gaaaagcaat accctgtatc
      661 tggaacgtgg atttgcccaa tttctcggca cgcttcgcgt gggtgatatg tacacatcaa
      721 gcgatatttt tgattctgtt cgcttcagcg gtgtgcggtt gtttcgtgat atgcagatgt
      781 tgcctaactc gaaacaaaat tttacgccac gggtgcaggg gattgctcag agtaacgcgc
      841 tggtaactat tgaacagaat ggttttgtgg tttatcagaa agaggttcct cctggcccgt
      901 ttgcgattac agatttgcag ttggccggtg gtggagcaga tcttgatgtt agcgtgaaag
      961 aggcggacgg ctcggtaacc acctatctgg tgccttatgc agcggtgcca aatatgctgc
     1021 aacccggcgt gtcgaaatat gattttgcgg cgggggcgag caagcaaagt gattttgtcc
     1081 aggcaggtta tcagtatggt tttaataact tattgacgct gtatggcggc acgatggttg
     1141 ctaataatta ctatgcgttt accctcggaa cgggttggaa cacgcgcatt ggcgcaattt
     1201 ccgtcgatgc cacgaagtcg catagtaaac aagacaacgg cgatgtgttt gacgggcaaa
     1261 gttatcaaat tgcctacaac aaatttgtga gccaaacgtc gacgcgtttt ggtctggcgg
     1321 cctggcgtta ttcgtcgcgt gattaccgga catttaacga tcacgtttgg gcaaacaata
     1381 aagataatta tcgccgtgat gaaaacgata tctatgacat tgccgattat taccagaacg
     1441 attttggccg taaaaatagc ttttctgcca atatgagcca gtcattgcca gaaggctggg
     1501 gttctgtgtc cttaagtacg ttatggcgag attactgggg gcgtagcggc agcagtaagg
     1561 attatcagtt gagttattcc aataactggc ggcggataag ctataccctc gcggcaagcc
     1621 aggcgtatgg cgagaatcat catgaagaga aacgttttaa tatttttata tcaattccct
     1681 gtgattgggg tgatgacgtt acgacgcctc gtcggcaaat atatatgtct aactcaacga
     1741 cgtttgatga tcagggtttt gcctcaaata atacgggatt atcaggaaca gtagggagtc
     1801 gggatcagtt caattatggt gtcaacctga gtcatcaaca tcagggaaat gaaacgacag
     1861 ctggggcgaa tttgacctgg aacgcgccgg ttgcgacagt gaatggcagt tatagtcagt
     1921 cgagtactta tcgacagact ggagccagtg tttcaggggg cattgtcgcc tggtcgggtg
     1981 gcgttaatct ggcgaaccgt ctttccgaaa cgtttgctgt gatgaatgcg ccaggaatta
     2041 aagatgctta tgtcaatggg caaaaatatc gcacaacaaa ccgtaatgga gtggtggtat
     2101 acgacggaat gacaccttat cgggaaaatc acctgatgct ggatgtgtcg caaagcgata
     2161 gcgaagcaga attacgtggc aaccggaaaa ttgccgcccc ttatcgcggc gcggttgtac
     2221 tggttaattt tgataccgat cagcgcaagc cctggtttat aaaagcgtta agagcggatg
     2281 gtcaaccatt aacgtttggt tatgaagtca atgatatcca tggtcataat attggcgttg
     2341 tcggccaggg aagccagtta tttattcgca ccaatgaaat accgccatcg gttaatgtag
     2401 caattgataa gcaacaagga ctttcatgca caatcacctt cggtaaagag attgatgaaa
     2461 gtagaaatta tatttgccag taatagtcag gtggttctat ggaaattcgc ataatgctat
     2521 ttatattaat gatgatggtt atgcctgtga gctatgcggc atgttatagt gagttatctg
     2581 ttcagcacaa tttggttgtt caggggaatt ttgcacttac tcaaacacaa atagcgacat
     2641 atgagcataa ttttaatgat tcgtcatgcg tgagtacaaa tactatcacc cctatgagcc
     2701 catcggatat tattgttgga ctttataacg ataccattaa attaaattta cattttgaat
     2761 ggaccaataa aaacaacatc acgttgtcaa ataatcagag cagtttcacc agtggttatt
     2821 cggtaacggt gacacctgcg gccagtaatg caaaagtgaa tatttctgcg gggggcggtg
     2881 gttcagtgat gattaatggt gttgcgacat tatccagtgc ttcatcatcg acacgcggga
     2941 gtgccgcagt acaatttcta ctgtgtttat taggtggcaa gtcatggggt gcatgtgtaa
     3001 atagctacag aaatgcattg gcacaaaatg caggtgtcta ttccttcaat ctgacattgt
     3061 catacaaccc gataaccaca acctgcaaac aggacgattt attaattact ttagacagta
     3121 ttcccgtttt acaattacca gccacaggta acaaagcaac aataaatagt aaaaaaaggg
     3181 atattattct gcgttgtaaa aatttattag gtcaacaaaa tcaaacatca cggaaaatgc
     3241 aggtgtattt atcaagttct gacttgttaa ccaacagcaa cacaatactg aaaggtgcgg
     3301 aagataatgc gtaggattta ttcttgaaag taatggttcg ccagtcacac ttttaaatat
     3361 cactaacagc agtaaaggat acacaaattt aaaagaaatt gcggcaaagt caaaacttac
     3421 agatacaacg gtttcaattc cgataacagc cagttactac gtctacgata caaacaaagt
     3481 taaatctggc gcactggagg caaccgcatt aatcaacgtg aaatacgact aataacaagc
     3541 aaaacgagca agtggctaat aataaaaaat aacaaggtgt ctgcatttcc ccctcatgat
     3601 gagggggctt tttttagcga tgataaaaaa tctcaccgtc gtaggcttta atgattttac
     3661 cgtcggtgtc gctgatcaac acgtacgcgc cacccatata ggtccagtgc gtcccggcat
     3721 caggcgcggg caggttacgt aactgccact gcttaatgtt gtactcatcg gtgcggtaca
     3781 tctctgggac ggtatcacct ggtttgaaat gcttaaagtc agcaatgaaa ctacttaact
     3841 catacttaga aattcccgag gtattggtcg aacccgcggt ggctggtgcg gcccaggcgg
     3901 cactggtaac cagcaaaagc acacccagca acatcttatt tttaatggtc ataaactctc
     3961 cactttgggg atctgtaata tcagtcgcat atctttcctg tcatacaata taactgacaa
     4021 gcgatatttt gttagtgatc ccatgatttc gtaaaaagtg taaatgaaag gatgccctaa
     4081 aagcatcctt agcgagcaga gagtcagcgg aaatagctga tttaagaatc atcctgggct
     4141 gaagataaca tgcaagccta aaggattgag ttatcgcatt atgcccatga agccatgtac
     4201 gcccatatac acaccgacta agccaatcaa cagactggaa aaataggggg cgcgtttagc
     4261 gagagtgtta aatccgctcc agcgttttgc gacctgctga acgctgattg ctgcgccaac
     4321 gcctacggtg acaagcgtta acgccaggcc aatgctgaaa ctgacgacca gtgttgcgcc
     4381 cagtgtcagg gctttcaact gaatgcatat caacagcacg gtaattgctg ccgggcaggg
     4441 gataaagcca ccggttaagc caaataacaa aatttgccag ttgatgacct ctctaccatc
     4501 aaagcgtcgt ttaatgtcat tggcatgggc tcgtgcatgg gcatcctgat actcgccatg
     4561 ttcgtgatgg tgatgatgtc cttggtcgtg gtgatgttcg tgatcgtgat gatggtgatg
     4621 atgctcataa tcatgcccgt gcatattctc cagccagttg cgttcgccgc gctaggtacg
     4681 ccagaacatc cagaacgcgg tgccaataat gatcactgcg gaaatcagct ggagccacgg
     4741 ttctgctgat tgagcagtaa agcgcttact gatcaccatc ccgccaaagg caatcaacca
     4801 gaccaccgcg gtatgcgaaa tggttgctgc cagtccgagc atcaccgcct gtttaatggt
     4861 gcctttgatg gcgatgataa acgccgccat catcgttttt gagtgccctg gttccaggcc
     4921 atgaagcgca ccaagtaaga tggcgctggg gatgaagaac caggcgtttc cttgctgaag
     4981 aagagttgta aattcggtca tgataataat tcttagtatt aattcggcaa tctgattcta
     5041 ctccccccca gtacctgata ctaccccccc agtagattaa tagtgctatg atttttcata
     5101 ttcttgttaa ccaggtgttg ccatgtctca tacaatccgt gataaacaga aactgaaagc
     5161 gcgtgccagt aagattcagg gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca
     5221 cgaatgcgct gcagttttac aacagattgc tgctatccgt ggagcggtaa acggtctgat
     5281 gcgggaagtg attaaaggtc atctgacgga acacatcgtt caccaggggg atgagctaaa
     5341 acgtgaagaa gatctggatg tcgttctgaa ggtgctggat tcatatatca aataatttat
     5401 taacgcgatt gtaaaactgc cgtttttcct cgtttacaac gggggcgctg gacattacca
     5461 tcctcctctg cgatttatca tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc
     5521 tgagaaatac ccgtatcacc tgatctggat aatgccagcg tagggaagtc acggaccacc
     5581 aggtcattgc ttcttcacgt tatggcagga gcaaactatg caagtcgacc tgctgagttc
     5641 agcgcaatct gcgcacgcgt tacacctttt tcaccaacat tcccctcttg tgcactgcat
     5701 gaccaatgat gtggtgcaaa cctttaccgc caataccttg ctggcgctcg gtgcatcgcc
     5761 agcgatggtt atcgaaaccg aagaggccag tcagtttgcg gctatcgcca gtgccttgtt
     5821 gattaacgtt ggcacactga cgcagccacg cgcacaggcg atgcgtgctg ccgttgagca
     5881 agcaaaaagc tctcaaacac gctggacgct tgatccagta gcggttggtg cgctcgatta
     5941 tcgccgccat ttttgtcatg aacttttatc ttttaaaccg gcagcgatac gtggtaatgc
     6001 ttcggaaatc atggcattag ctggcattgc taatggcgga cggggagtgg ataccactga
     6061 cgccgcagct aacgcgatac ccgctgcaca aacactggca cgggaaactg gcgcaatcgt
     6121 cgtggtcact ggcgaggtgg attatgttac cgatggacat cgtatcattg gtattcacgg
     6181 tggcgatccg ttaatgacca aagtggtagg aactggctgt gcattatcgg cggttgtcgc
     6241 tgcctgctgt gcgttaccag gcgatacgct ggaaaatgtc gcatctgcct gtcactggat
     6301 gaaacaagcc ggagaacgcg cagtcgccag aagcgagggg ccaggcagtt ttgttccaca
     6361 tttccttgat gcgctctggc aattgacgca ggaggtgcag gcatgaaacg aattaacgct
     6421 ctgacgattg ccggtactga tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc
     6481 ttctcggcac ttggcgctta tggttgctca gttattactg cactggtggc gcaaaatacc
     6541 cgtggcgtac agtcggtgta tcgcattgag cctgattttg tcgccgccca gctcgattcg
     6601 gtgttcagcg atgtgcgaat cgataccact aaaatcggta tgttggcgga aaccgatatt
     6661 gttgaagcgg tggcagaacg gttgcaacgt tatcagatcc aaaacgtggt actcgacacc
     6721 gttatgctgg caaaaagcgg cgacccgctg ctttcacctt cggcggttgc tacgctgcgc
     6781 agtcgattat tgccacaggt ttcattaata acgccaaact tgcccgaagc tgccgccttg
     6841 ctcgacgcgc cacacgcgcg caccgaacag gaaatgctgg aacaagggcg atcgctgttg
     6901 gcgatgggct gtggcgcagt gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg
     6961 gactggctgt ttacccgcga gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa
     7021 aacacccacg gcactggttg tacactctct gcggcgttgg ctgcactacg cccgcgccat
     7081 acaaactggg ctgacaccgt acaggaggca aaaatctggc tttcatcggc gttagcccag
     7141 gccgacacgc tggaagttgg tcacggtatt ggtccggttc accacttcca cgcctggtgg
     7201 tgagcaccgt actatagctg ctttggttat tgtcagcctt tggcactgac attcagggat
     7261 gaaaccaatg cagctaagaa ttaccagtcg aaaaaaactc accgctctat tatgcgctct
     7321 tgggctaatt tccatcgttg caatttatcc gcgtcagacg gtgaattttt tctattcgac
     7381 agcagttcag attacagact acatccactt ctacggttat cgcccggtta aatctttcgc
     7441 tattcgcatt cctgccagtt acaccattca cggattagat gtttcgcgct ggcaggagcg
     7501 tatcgactgg cagcgggtgg caaaaatgcg cgacaacgat atccgcttac agtttgcttt
     7561 tattaaggct acggaaggcg aaaagctggt ggacccctat ttttcgcgta actggcaatt
     7621 aagccgcgaa aatggcctgc tgcgcggggc gtatcattat ttctccccgt cggtatccgc
     7681 ttcagttcag gcgcgattat ttctgcaaac ggtggatttc tcacaaggcg atttccctgc
     7741 cgtgctggac gtagaagaac ggggaaaatt atcggcaaaa gaattacgca agcgggtaag
     7801 tcagtggcta aaaatggtcg aaaaacgcac gggaaaaaag ccgattattt actcaggagc
     7861 cgttttttat cacaccaatc tggcgggcta tttcaatgaa tatccgtggt gggtggctca
     7921 ctattatcaa cgtcgtccgg acaatgacgg catggcctgg cgcttctggc agcattccga
     7981 ccgtggacag gtagatggca tcaatggtcc ggtggatttt aatgtgttta atggcacggg
     8041 gatgagttgc aggcattcgt tgatgggatt aaagaaacgc cttaaataag aagcagtgat
     8101 atactcccac cagactccct ggcaggaaaa agacaaatgg aacaagcgca tacccagctg
     8161 attgcccaac tgaacgagcg tattttagcg gcggataaca cgccgcttta tattaagttt
     8221 gccgaaacgg taaaaaatgc cgtgcgcagc ggggtgctgg agcatggcaa tattttgccc
     8281 ggtgagcgtg atttaagtca gttaaccggc gtttcgcgca ttacggtgcg caaggcgatg
     8341 caggcgctgg aagaagaggg cgtggtgacg cgttcgcgcg gttacggcac gcagatcaac
     8401 aacatcttcg aatactcgtt gaaagaagcg agaggttttt ctcagcaggt ggtattgcgc
     8461 gggaaaaagc ccgatacgct atgggttaat aagcgtgtcg tgaaatgtcc cgaagaagtt
     8521 gcgcagcagc tggcggccga agcgggaagt gatgtctttt tgcttaagcg tattcgctat
     8581 gtcgatgaag aagcggtatc gattgaggaa tcgtgggttc cggcgcattt aattcatgat
     8641 gttgatgcca tcggaatttc tctttatgac tatttccgca gccagcacat ttacccacaa
     8701 cgtacgcgtt cccgcgttag cgcccggatg ccggatgccg agtttcagtc acatattcag
     8761 ttagatagca aaataccggt gctggtgatc aagcaagtgg cgcttgacca acagcaacgg
     8821 cctattgagt acagcattag ccactgtcgc agcgatctat acgtttttgt gtgcgaggag
     8881 tagttcctcg cgcgttggcg cacaatcacc gccccgatgc ccgacaaccc acgacgccac
     8941 tgcattgccc agtaatacgg catccgccag tggcagacca gaggccagcc cggcaagtac
     9001 gccacctgca tgactgtcgc ccgccccaat ggtgtctaca acttgcgttg gaaatgccgg
     9061 aatgcagcca gaagcgtcgt tgctgaaata ccatgcgcct tctttatcga gacgaacgat
     9121 caacggcgcg gcaaatttct cctgccattg cttgccaagt gttgttatct ctgcggataa
     9181 agcaaaacgt tcggcggcaa tctcagcctc ttgacgattg agtgacacta aaggtcgaca
     9241 ggccatgatc cgcgccagta atgcgtccgg gatatcgcca atacgtgggc aaaaatcgat
     9301 aaacggcgtc acgtcttgca gcttttccag ccattccact aacaattcgc cgcagggcga
     9361 ggccagttga taaccggaaa aatagagcag gttgccaggc gcaacggtta atcgtgccaa
     9421 ccattggcga ttccattgat tttccacacc gctgaatgac ataaaagtgc gttcgccatc
     9481 cggctcaacc agtgccagac accagccgtt atcgctttcg gcgttatcga tcaggctgat
     9541 taacccctct ttcgccatcc ggttgcgaat aatctccgcc cacacgccct gaccgagcgg
     9601 caaggcatta cccgcttcga tgccgaggcg ctttaacgcc acggcaatat tcagtgcgca
     9661 gccgccaacg ttaacgctct gctgtttcag ttcgatatcg cacccacgcc aggggagggc
     9721 ataagcgtcg gcgatcacgt caatgaccgc cgcgccgacg accatcaccg gctgacgcgt
     9781 ggttaattca ggcagcagtg tgtgtaatcg agcgccgctc ataccgcctc ccgttgttga
     9841 cgatatttcg ccagcgctgt ggcatagcgg ttgaaatcaa gctgatttac cgcatccagc
     9901 tccgccttta acgcaggatc gacagcgtta atgccatgca acgcgccgca aatcgccgtc
     9961 gccatagcac ctatggtgtc tgtgtcgccg ccaagattgg cgcacaggac ggcgcagcgg
    10021 tttgggtcgg tttgtgccag ttcaactagc gcaatggcgc acggaacgga ctcaatagtg
    10081 ctggtgcctg cgccaacgac ctggtaaagc tgttcgctgg cggattcggt gccgtcggca
    10141 ttacgcacaa ttttcagcgc aatctccaga cgtgccgcca gtgaggcgct gaacgtggtg
    10201 gtgcgttttt gctgtgcatg tcgcgcaatt gaaggcaatg aatcaacaat cgctgaccag
    10261 cgttctccgt caatggcacg agaaatcgcc catgcgatga ctaccgcgcc tgcaaccgcc
    10321 agatcggatt tatgggtcgg gctggacgcc agcgccacat catcaataaa ggaatcaaca
    10381 tcacgcgctg gaagcaaaca acctaatggc gagacgcgca tcgctgcgcc gttggttacg
    10441 ccgttgtttt ccagttcagc aacgggttta ccgtcgcgaa tggcgttaag cgcaatcttc
    10501 gaggtcggac ctagtacgtt tttgttaaag gcgtcgaaac gcaacgccca gtcgagaata
    10561 ttacgcccaa tcagattcgg atcgatcttg ccttcacatt ccagtaacgc atccgccaga
    10621 cacaggccat cgaggtatcg tcggtgaatt cggcgcggtt aaaataacag gccgcgttat
    10681 tctcctttgg tccaggcaga aaacggtcaa tccagccaaa gtgtgcttta acgcggctgc
    10741 gtggccaaag ctcggagggc atccccatcg catcccctaa cgcctgccca taaagagcac
    10801 cgagaatacg ttctgttttc atttaacttc cccttgaatc ggaatttacg ggagcatagt
    10861 aatgacagat attgcgcagt tgcttggcaa agacgccgac aaccttttac agcaccgttg
    10921 tatgactatt ccttctgacc agctttatct ccccggacat gactacgtag accgcgtgat
    10981 gattgacaat aatcgcccgc cagcggtgtt acgtaatatg cagacgttgt acaacaccgg
    11041 gcgtctggct ggcacaggat atctttctat tctgccggtt gaccagggcg ttgagcactc
    11101 tgccggagct tcatttgctg ctaacccgct ctactttgac ccgaaaaaca ttgttgaact
    11161 ggcgatcgaa gcgggctgta actgtgtggc atcaacttac ggcgtgttgg tgtcggtatc
    11221 gcggcgctat gcgcatcgca ttccatttct cgtcaaactt aatcacaacg agacgctaag
    11281 ttacccgaat acctacgatc aaacgctgta tgccagcgtg gagcaggcct tcaacatggg
    11341 cgcggtggcg gttggtgcga ctatctattt tggctcggaa gagtcacgtc gccagattga
    11401 agaaatttct gcggcctttg aacgcgcgca cgagctgggc atggtgacag tgctgtgggc
    11461 ctatttgcga aactccgcct ttaagaaaga tggcgttgat taccatgttt ccgccgacct
    11521 gactggtcag gcgaaccatc tggcggcaac cataggtgca gatatcgtca aacaaaaaat
    11581 ggcggaaaat aacggcggct ataaagcgat taattacggt tataccgacg atcgcgtgta
    11641 cagcaagtta accagcgaaa acccgattga tctggtgcgt tatcagttag ctaactgcta
    11701 tatgggccgg gctgggttga taaactccgg cggtgctgca ggcggtgaaa ctgacctcag
    11761 cgatgcagtg cgtactgcgg ttatcaacaa acgcgcaggc ggaatggggc tgattcttgg
    11821 acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa ctgattaatg ccgtgcagga
    11881 cgtttatctc gatagcaaaa ttactattgc ctgatacgcc tcatacgaac agcaccaccc
    11941 cgtaggacgg ataagacgtt tacactgcat ccggcaacct atgcctgatg cgacgcggaa
    12001 gcgtcttatc atgcctacat agcactgcca cgtatgttta caccgcatcc ggcataaaaa
    12061 cacgcgcact ttgctacggc ttccctatcg ggaggccgtt tttttgcctt tcactcctcg
    12121 aacaattttc atattgttgt ttttgcgatc attatctcga catttaaaag caaataattt
    12181 cattatattt tgaaatcgaa agcaaacgac aagatatgaa aatgtacgtg gtatcgacaa
    12241 agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca
    12301 atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca
    12361 tcgccggaac gcctggcaca tttactcatg ctggtacaga aaatctgttg gcgctggtca
    12421 gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat
    12481 ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc
    12541 atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct
    12601 ttgatgtcag cgtcgaggcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag
    12661 tcaatgaagt cgatgcgttg tacaccaacc ccgcacaggc gcgtgaattt gccgaggcaa
    12721 ccgggattga ttccctggcg gtcgccatcg gcacggctca cggtatgtat gccagcgcac
    12781 cggtgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc
    12841 tgcatggcgc gtcaggatta tcgactaagg atattcagca aaccatcaaa ctggggatat
    12901 gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctt gcagtcgtta aaaaattacc
    12961 tgaccgagca ccccgaagcg accgatcccc gggattattt gcagtcggct aaatccgcaa
    13021 tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca
    13081 cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa
    13141 catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca
    13201 tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa
    13261 tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc
    13321 gataaagttg ggtttgcacg cgaacgcatt attctcggcg gcgatcatct gggaccaaac
    13381 tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca
    13441 tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat
    13501 accataccgt tagcaccaga aacggttgcg gaacgagctg ctgtgctttg ctttgctgcg
    13561 gaaagtgtgg cgacagattg ccagcgtgag caactgagct atgtcattgg cactgaagtt
    13621 ccggttccgg gcggtgaggc cagcgccatt cagtcagtac acatcaccca tgttgaagat
    13681 gccgccaata ctttacgtac gcatcaaaag gcctttattg cccgtgggct gacagaggcg
    13741 ttaacacgtg tgattgccat cgtggtgcag ccgggtgtgg aatttgatca cagcaatatt
    13801 atccattatc agccgcagga agcgcaggcg ctggcgcaat ggatagaaaa cacccgaatg
    13861 gtttatgaag cacattctac cgattaccag acccggacgg cttattggga attagtccgc
    13921 gatcactttg caatattgaa agtcggtccc gcattaacct ttgctttacg cgaggcgata
    13981 tttgcactgg cacaaattga gcaggaactt atcgcccccg aaaatcgcag cggttgcctg
    14041 gcggtaattg aagaagtgat gctcgacgaa ccgcaatact ggaaaaaata ttatcgcacg
    14101 ggttttaacg attcattact ggatattcgt tacagcctgt cggatcgtat tcgttattac
    14161 tggccgcata gtcggattaa aaatagcgtt gaaacgatga tggtgaatct gcaaggcgtg
    14221 gacatcccac tgggcatgat tagtcagtat cttcccaaac aatttgaacg cattcagtcc
    14281 ggggaattat cagcaatgcc gcatcagctt attatgaata aaatttatga tgttttgcgc
    14341 gcctatcgct acggctgtgc ggaataagga cggtatatga ctaacctgtt tgttcgtagc
    14401 ggaatttctt ttgtcgatcg tagcgaagtt ttaacctata tcggtaatga gatgctcgcg
    14461 aaaggtgtgg ttcatgatac ctggccacag gcattaattg ccagagaagc agaattccct
    14521 accgggataa tgcttgagca gcacgccatt gcaataccgc attgtgaggc gattcatgct
    14581 aagtcgtcag ccatttatct gttaaggcca acaaataaag ttcattttca gcaagcggat
    14641 gatgataacg acgtggcggt atcgttggtt attgcgttga ttgtggaaaa tccgcagcag
    14701 caattgaaac ttttacgctg tttatttggc aagttacaac agcccgatat cgtcgagaca
    14761 ctaattactc ttcctgaaac tcagttaaag gaatacttca caaagtatgt tttagattca
    14821 gacgaataaa tccctctgta acaataaaaa ggactattta tgaaacgcaa gattattgtc
    14881 gcttgcggag gcgcggttgc gacctctgcg atggcggcgg aagaaattaa agagttgtgt
    14941 cagagtcata atattcctgt tgaattaatc cagtgtcggg ttaatgaaat agaaacctat
    15001 atggatggtg tgcatttgat atgtaccact gccagggtgg atcgtagttt tggcgatatt
    15061 ccgttagttc acggcatgcc ttttgtttct ggtgtcggta tcgaagcatt acaaaataaa
    15121 attctgacta tcttacaggg atgacctatg ttttcagaag tcatgcgtta tattctcgac
    15181 ctcggcccta cagtgatgct gccggttgtc atcattattt tttctaaaat attaggcatg
    15241 aaggcaggcg attgctttaa agcgggtctg catatcggga ttggctttgt tggcattggc
    15301 cttgtgattg gcttaatgct ggattccatt ggtccggcgg cgaaagcgat ggcggaaaat
    15361 ttcgacctga atctgcatgt ggtcgatgtc ggctggccgg gctcttcacc aatgacctgg
    15421 gcgtcgcaaa ttgcgctggt ggcgattccg attgcgatat tggttaacgt ggcgatgtta
    15481 ctgacccgta tgacgcgggt ggtaaatgtt gatatctgga atatctggca tatgaccttc
    15541 accggcgcgt tgctgcacct ggcaaccggt tcatggatga tagggatggc gggtgtggta
    15601 attcacgcgg cgtttgttta taagctcggc gactggtttg cccgcgatac ccgaaatttc
    15661 tttgagctgg aaggtattgc tattccgcac ggtacgtcgg cgtatatggg gccgattgcg
    15721 gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta accgaattaa atttagcgcc
    15781 gacgatattc agcgcaaatt tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg
    15841 gggctgatta tcggcatcct cgcgggttac gatgtcaaag gtgtattgca gctggcggta
    15901 aaaacggcgg cagttatgct gctgatgcca cggatgatta aacccatcat ggatggttta
    15961 acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc
    16021 ctgattggcc tggatcccgc attactgctg gggcatacgg cggtggtatc ggcaagcctg
    16081 atttttattc cgctcaccat tttaattgct gtttgtgttc cgggtaatca ggtactgccg
    16141 tttggcgatc ttgccactat cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga
    16201 aatctgttcc gcaccttaat ctcgggtgtc atcattatga gcatcaccct gtggatcgcg
    16261 acgcaaacta ttggtttgca cacccaaccg gcggctaatg ctggggcgtt aaaagccggg
    16321 ggtatggtgg cttcaatgga tcagggcggt tctcccatta cctggttact gattcaggtt
    16381 ttctcc
//
LOCUS       DAJTRH010000083        16316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTRH010000083 DAJTRH010000000
VERSION     DAJTRH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..331)
                     /gene="chbA"
                     /locus_tag="OO339_002953"
     CDS             complement(<1..331)
                     /gene="chbA"
                     /locus_tag="OO339_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5927507.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIEL"
     gene            complement(390..1738)
                     /gene="chbC"
                     /locus_tag="OO339_002954"
                     /pseudo
     CDS             complement(390..1738)
                     /gene="chbC"
                     /locus_tag="OO339_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(1823..2143)
                     /gene="chbB"
                     /locus_tag="OO339_002955"
     CDS             complement(1823..2143)
                     /gene="chbB"
                     /locus_tag="OO339_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5927508.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2442..2780)
                     /gene="osmE"
                     /locus_tag="OO339_002956"
     CDS             complement(2442..2780)
                     /gene="osmE"
                     /locus_tag="OO339_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5927509.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            2982..3809
                     /gene="nadE"
                     /locus_tag="OO339_002957"
     CDS             2982..3809
                     /gene="nadE"
                     /locus_tag="OO339_002957"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5927510.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4039..4926
                     /gene="cho"
                     /locus_tag="OO339_002958"
     CDS             4039..4926
                     /gene="cho"
                     /locus_tag="OO339_002958"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5927511.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(4886..5461)
                     /gene="ves"
                     /locus_tag="OO339_002959"
     CDS             complement(4886..5461)
                     /gene="ves"
                     /locus_tag="OO339_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5927512.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(5664..6149)
                     /gene="spy"
                     /locus_tag="OO339_002960"
     CDS             complement(5664..6149)
                     /gene="spy"
                     /locus_tag="OO339_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5927513.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6479..7447)
                     /gene="astE"
                     /locus_tag="OO339_002961"
     CDS             complement(6479..7447)
                     /gene="astE"
                     /locus_tag="OO339_002961"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5927514.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7440..8773)
                     /gene="astB"
                     /locus_tag="OO339_002962"
                     /pseudo
     CDS             complement(7440..8773)
                     /gene="astB"
                     /locus_tag="OO339_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(8770..10248)
                     /gene="astD"
                     /locus_tag="OO339_002963"
     CDS             complement(8770..10248)
                     /gene="astD"
                     /locus_tag="OO339_002963"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5927515.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10245..11279)
                     /gene="astA"
                     /locus_tag="OO339_002964"
     CDS             complement(10245..11279)
                     /gene="astA"
                     /locus_tag="OO339_002964"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5927516.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11276..12496)
                     /gene="astC"
                     /locus_tag="OO339_002965"
                     /pseudo
     CDS             complement(11276..12496)
                     /gene="astC"
                     /locus_tag="OO339_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            12942..13748
                     /gene="xthA"
                     /locus_tag="OO339_002966"
     CDS             12942..13748
                     /gene="xthA"
                     /locus_tag="OO339_002966"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5927517.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            13915..14625
                     /locus_tag="OO339_002967"
     CDS             13915..14625
                     /locus_tag="OO339_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5927518.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            14630..15307
                     /gene="ydjY"
                     /locus_tag="OO339_002968"
     CDS             14630..15307
                     /gene="ydjY"
                     /locus_tag="OO339_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5927519.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15322..16029
                     /locus_tag="OO339_002969"
     CDS             15322..16029
                     /locus_tag="OO339_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5927520.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16029..>16316
                     /locus_tag="OO339_002970"
     CDS             16029..>16316
                     /locus_tag="OO339_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5927521.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDK"
BASE COUNT         4018 a         4353 c         3995 g         3950 t
ORIGIN      
        1 gaagctcaat taattcagta atcagttcac gcgcaagcat ggacgtcatt aaatgatcct
       61 gagcgtggac cagcaccaga ctcactttca tcttaccttc gcccgcatcg ccttcaatca
      121 gtttcgtctg taccagatgc gcttcattca atgccatccg tgactgatcc atcatggctt
      181 tcgctgcggc aaaatcgccc tgcttcgcct gtttcaatgc ggcatacgcc aggctacgcg
      241 cttgtccaga gttgatgata agccccatca ccacttcttc cagctcttca gcttctgttt
      301 gcgtatcagg aatattatcg agatccatca tacatcgttc ctctctcttc ttaccggcaa
      361 gattacccgt accggcatcg attaaaattt caggggttag cgatatcttc ttcgctctct
      421 tctttatcaa tcgcattttg tgctttgtta gccaccacaa caaagggcag ataaattaac
      481 gttgcgatgc caaggttgaa gagtgcgacc agcaatgcgg cgacgctacc gttggtgtta
      541 aagaaggctc ccagaccggt tggcatggtc cacggtgcaa tattggtcac cggaggaatg
      601 atgcccatgt agtacgctgc gagggtgatt gccgccagaa tcggttgtac cagtacaaac
      661 gggataaaca tcaccgggtt catgataatt ggcagaccaa acagaatcgg ttcgttaatc
      721 tggaagatgc cggacggcag cgccagtttt gccacctgac gataatcagc acggcgagag
      781 gcgataaaga tagccaggat caggcctaaa gtcgcaccac tgcccccaag gaaaataaag
      841 gagtccagca tcggcttggc ccagatatgg aaggtcttac cggctgccag cgccgcttcg
      901 acggaaccat attgctgata ggtcgcgata ttttccagcg cccacggcgt cataatgccg
      961 ttgtccagtg cggtcagcgc cagcgcgcca tgaataccga agaaccagag cagtggaaca
     1021 aagatcacat aggcccagcc caccacgcta cccaacgatg ccagtggggt tgagatggta
     1081 tccataatga tctgatggaa gttggtgccc caggtattca acgcccaggc aataatcccc
     1141 atcacggaaa gaataataaa gccgggaatt aatgccgaga aagaacgcga tactgaagca
     1201 ggtacgctgt cgggcagttt aatgacccaa ttgcggcgga caataaaggt aaacatttct
     1261 gccaccacca ggccaataat aatcccggag atgatattcg ccccacctaa ccagtttgcg
     1321 ccaaccgcat aggcctcacc gacactatat ggggtgacgg tcataaatgc tgcaacggat
     1381 aacaacccag ccgccagcgc atcgacttta cgctcttctg ccagcgccat gccaataaag
     1441 aacggtgcca ttaaagacat tattcctaat gttccgttat atacgttgcc gccaatacct
     1501 ttcagaccat taagtgtttc aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa
     1561 acgacccctc cccaaagctt agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa
     1621 gggttaacgg cattaagcga ataaagccat ttttgattgc attaacgtgt ggctgctttc
     1681 ctattttaac tgcaaaaggg aggagtacct tttcaagcga tgcaataaca ttactcatag
     1741 aaaaataccc ttaaaaaccg caatttaaat attacggtat tgatttatga aataactctt
     1801 tgacgggaaa atttaaaata aattaatttg ctgcggcttt tttaatcgct gcaaccgcag
     1861 ccttaagcac gcctaaacca tcgactttgc cataaagcag cgagtcaatt acttcaaccg
     1921 gtttgttggg taacaaacgc tggatttcgg gcaacatata agcaatctgc ggccctaata
     1981 acacgacatc ggcattctga cctttttcac cagccagtgt ttccggaaat gcttcaataa
     2041 tgaccggaac ttcatatttt tctgcctgtg cgcgcatttt tgataccagt aaagagatag
     2101 acatgcccgc agaacaaaac agataaatgt gtttcttttc cataaaactg ccctcatcga
     2161 cgattatctg tcagccagac actccgcaag ccttgacctg cttccatgct ctgggtaact
     2221 tgcgaaacca acatgacttt ttttgtggct gaaaggagta tacggtatag acctgagcaa
     2281 tagtatttct ttgaccttat aaattgtttc tccttgacct gtggttatga ccctcttcac
     2341 atttcgggca aataattcgc gaagataatt aagaactcag gccaaaaaaa accggcgcaa
     2401 tggccggttt ccgttgttac tcaagctttc agacgaattg attacttcgc agcctgtgga
     2461 tcagtgtcgt attcagcaca ggtctgataa ccggagttga tgacatgtcc ggtatcatct
     2521 aacgcgacaa agtaggtttc tgctttacca tcacgttgac ccaggatgta ggtctggcaa
     2581 gtaccgcgag catggatcat gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc
     2641 tgcgcccggc tcatgccttt tttgacgtct ttcaccacag gctgtacaaa ctggtctttg
     2701 gtacgatcat aagctgtaca acccgccagc atggttaata ccgccgctgc actcagaatt
     2761 cctgccatat tcttgttcat attccgtcct cttgtttatc agcgtgttag ataagcctgg
     2821 aatacattgg gcgctttttc aagcccgtga acgaaacggc tccgctttca gaggattcct
     2881 gtatgacgtt ttaaccacca ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt
     2941 agctttaagg aagttttgtc ttttctgtct ggaggggttc aatgacattg caacaacaaa
     3001 taataaaggc gctgggcgca aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg
     3061 tcgattttct gaaaagctac ctgcaaactt atccgttcat taaatcactg gtgctcggga
     3121 tcagcggcgg tcaggactcc acgcttgccg gaaagctgtg ccagatggcg attaatgagc
     3181 tgcgccagga aactcgcaac gaatcactgc aatttattgc cgtacgcctg ccctatggtg
     3241 ttcaggctga cgaacaagat tgccaggatg ccattgcctt tattcaaccg gatcgcgtat
     3301 taaccgttaa tatcaagggc gcggtattgg ctagcgagca ggcattgcgg gaagcaggca
     3361 ttgaactgag cgattttgtc cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat
     3421 atagcattgc gggtatgacc agcggtgtcg tggtgggcac cgatcatgca gcagaagcca
     3481 ttaccggatt cttcactaaa tatggtgacg gcggtacgga tattaatccg ctgtatcgtc
     3541 tcaacaaacg ccagggtaaa cagttactga cggcattagg ttgcccggaa cacctttata
     3601 agaaagcccc aactgccgat cttgaagatg atcgcccttc tctgccagat gaagtggcac
     3661 tcggcgtgac ctatgacaat atcgacgact atctggaagg gaaaaaccta cctgaacagg
     3721 tcgccagaac aatagagaac tggtatctga aaaccgaaca taaacgccgt ccgccaatta
     3781 acgttttcga tgatttctgg aaaaagtaat aatttgcacc tattggattg tgcgaaaaag
     3841 agtaatttgt tcacgccgga tgcggcgtga acgccttatt ctacctacaa aactatgcaa
     3901 attcaataca ttgcaggagt cgaataggcc tgacaggcgt agcacgtcag acggtgtaac
     3961 ctttgtcatc aacccgcctc ttttttaatc gcctcccgcc tgttacactg gatagataac
     4021 cagcattcgg agtcaacagt ggtacggcgt ttaacttctc cgcggctcga atttgaagct
     4081 gcggcaattt atgaatatcc cgaacattta cattcattcc ttaatgactt acccacccga
     4141 ccaggggtgt atctgtttca tggcgaaagt gacaccatgc cgctctatat cggcaaaagc
     4201 gttaatatcc gcagtcgggt gctttctcat ttacgcaccc cggatgaagc cgccatgcta
     4261 cggcaatccc ggcgaattag ctggatctgc accgcaggcg aaatcggcgc tctgctcctt
     4321 gaagcgcgat taatcaaaga acaacagccg ctgtttaata aacggttgcg ccgcaatcgc
     4381 cagctctgtg ccctgcaatt aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg
     4441 gatttttcac gagcccccaa cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa
     4501 gcattgcaga gcatcgctga tgaacaaaaa ctttgttatg gcctgttggg actggaaccg
     4561 ttaagtcgcg gtcgtgcatg ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc
     4621 ggtaaagaga gccatgagga acatgcgcta cgcttgcgcc agtctctgga gcgtttgcga
     4681 gtggtgtgtt ggccttggca aggtgcggtg gcactgaaag aacagcaccc ggaaatgact
     4741 caatatcata ttattcaaaa ctggctgtgg ctgggggcgg ttaattcgct ggaagaagcg
     4801 acaacgttaa ttcggacacc cgccgggttt gatcacgacg gttataaaat tctttgtaag
     4861 ccgctgcttt ccggtaacta tgaaattact gaacttgatc cggcgaatga ccagcgagcc
     4921 agttgatttc actgaacaac agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc
     4981 catcgaacca gcacacgccc tggtcggtag tgagcagttt gtcgcccagt tgccacgccc
     5041 cgttaatcac aaacaccacc ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa
     5101 ttctgacttt cgccttacag acatccaggc gcgtcataat attgaaatcc atcgacatct
     5161 gccctgccgt cagtttcgct tttaccacct ggtccgccgt aaaggcaaaa ggctgtaacg
     5221 gttttaaggt atggttaaag cggtctgcgc tttcaaggag catctcgccg ccttccagca
     5281 acgtcactat cctttccatg ccgggaaaca aagaaaactc cccattcgcc gcgatggatg
     5341 caatgctggc acgccagtaa aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg
     5401 tttcaccggc agcgtttcgc cacagattca ccgacatttt acgcatatca aagtattcca
     5461 tactcgctcc cggcccgttc ttttcattgt tatcgttcag gtaaccgtgg atggttatcg
     5521 gcaactcggg tgatttacgt tagtggtgat caggcttttt ttacaactgt ttgagaagag
     5581 aaaagaaaac cgccgatcct gtccaccgca ttactgcaag gtagtggaca agaccggcgg
     5641 tcttaagttt tttggctgaa agattattca gcagttgcag gcattttacc ttttgccgct
     5701 ggacgttctg tcagacgctt ctcaaaatta gcattaaatt gctttttctg ttccggcgtc
     5761 aggatgttgt aaattttgtt ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt
     5821 tcttccattt ttgcgatctg cgcttcagct tttgctttat cgaaggtatc gctggcaatg
     5881 atgtcatgca ttgcgcggcg ttcttccagc ggcggacgtt tcatctggtc acgctggcct
     5941 ttcatgattt cgtggatctg ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc
     6001 atgtcctgat gcggaccgaa cttgcctttg tggtgcatca tcggcttcgc gtcagccggt
     6061 gctgcggtag tggtgtctgc ggcatgggcc aggttagccg cgccaagagc cagggtagag
     6121 gcaacaaaca gtgcagttaa tttacgcata ttctatatcc ttcctttcag ttatttatta
     6181 cggctttctt aagtagcgtg ccgtgttgac gagattaact ttactgactt tagcgtcaat
     6241 taatcagagc aacggtaaaa caatgaaagt gtaaaaaaca cttttgcgcc aattatggag
     6301 aaaaaaagaa aatttgatgg agagtgatga gagaatatta caacacgatg attttgcaga
     6361 gattatgaag aactataccg gatgactggt gataaataaa gcaaataacc aggattaatc
     6421 tgtattaatt tataagaaag caacttaata cccgcagaat gatttctgcg ggtaagtatt
     6481 agcttatttt ttcgagcatt aatcccgcgc gtaatcccaa cgctaccaac ggattaggga
     6541 ataacacata ctctacatca tgggttacgg taaaacgttc ctctccgtcc tgcgccagca
     6601 atgttccttt ctcaaacggc ataaaattca gcgtgtcact tgccatatgc atttcgaagg
     6661 acggcgagtg gcgagtaatt tgcgaaacca cccgataacg gagcggcggt gttctcacga
     6721 taccgacact ctcaccagat agcagcgcag caattgcgct ggcagttact gcaaactggc
     6781 gaagatcgtt ttgcccaaag ggcaacgctt tgccaagttc caacgtacag gccagcgcgc
     6841 caaaatgtct gacgctgaaa tgggtaaacg taccaccagg ctcctgatgg aacaccagcg
     6901 cctccagccc cgccgcaccc agccacgtca gaaatttctc gtcccaggga atgtcgcgtt
     6961 gcggtaatac accgaactgc ggatgcaagg agccacgaat tgcggtatgt agatcaaggt
     7021 gccaacgcac agattctttg ccctggtcat aaaaatcttc caggcactgt tccagttcgc
     7081 gcgcccgaca ggtttctccg ctttcagcaa atagctgcca acgaccgcca aacattcggt
     7141 tcatatcgct atggcaataa cgtttccctt gcttcagcgc aggaggattc ccgaggatca
     7201 ccagcaaccg ccaacgtaac gggatctcgc cgtgagatat cgcgccaagc aacgcgtcca
     7261 gcatttccac aggtgccgtc tcattaccgt ggatccccgc agaaatcacc agcgcgcctt
     7321 gcggtggcgt taatggcgtc agttccagca caccatcgcc cagccagcgc cagcgaacgc
     7381 cgttgatttc tcgctcggtg ataaccggtt ttttacccgt taaggtcaga gcaagaaaat
     7441 tatccattgc cccctccctc gcgctggaac ggataaaccg aaccgagatt cagtaattgg
     7501 ctcaatacat ccagtgcttc ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca
     7561 gcagtaagac gatcgcggta gtaacgatcc acccagtcat tgagcgcatt aaacaaggta
     7621 tcgttcatca tcaccgccgg attcaccgcc cggcgttctt cttctgtcaa taccacccgc
     7681 aaccgcaggc acgccgggcc gccgccattc gccatgcttt cacgtaagtc aaagactttt
     7741 agttcgctaa tcgggttgtc agcggcaagg agttcattga gataacccca tactccggcg
     7801 tgttcccgac actcctgagg cagcaccaac atcatggaac catcatcgcg gctcaacagt
     7861 tggctgttaa acagataggt agacaccgca tccgacacgg aaacctgagt tgccggaact
     7921 tctatcgcca taaaaccgtt cacccgcgtg ccagtaactg tgactggcga gcgaacgcct
     7981 gttggtggca aaacagcacc tggcggttac tcacggcaat cacgtcatta tgaaaaacgc
     8041 tctggtcgat aacgtccggg ttttgctggg cgaaaatcac ctgttgggga ttcacctgat
     8101 taagccttgc caccgcctcg ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc
     8161 gggtatcatt gccttcttct cgcccgtaga caaaaagtag catacccggt tcaccgtaat
     8221 gaccgccgag acgattgtgg tttgccgccc cctcatcacc gagcaacgct acctgcggca
     8281 acgccgaatg gacgctaaat ttctcttcgt ccttaaaaat cgcttttaac agcgattcag
     8341 tgacgggcgc ttccagcgaa cggtgaaatt tattgttcag gttggcaacg gtgagatgca
     8401 ctttgccatc cagcgtatcg gcagatggcg cgatcgttgc cgcattggct acccacattg
     8461 gcgaagcgga actgacgctg gaaagccagt gcggtgcctg gcgtgcaact ttttccagta
     8521 cctgctcatc gctaccactg aatcccaact gacgcagcac cggaataaac ggacgctcgt
     8581 gcggcggaat cacggcctgg gggaatcccg catcggcaag ggctttcatt ttcagtaagc
     8641 cctgttttgc cgccagtcgc gggttagaca cctgaaaacg gtgacgggta gaggcttcat
     8701 taccaaacga caggcccgcg taatgatgcg tcagccctac cagcccgtcg aaattaactt
     8761 cccaggcgtt catcgcacca cctcatcgga aaaatccagc ccggggttaa gcgtggcggg
     8821 caatgttaac gagtccgact ccaggctcgc cataggccat gcgcagtaat ctgcggcata
     8881 ccaggcgctg gggcgatggt taccggaagc accaatgccg ccgaatgggg cggtactggc
     8941 agcaccagta agcggtttgt tccagttaac aatccccgcc cgcgcctcca gcaacagttg
     9001 atcgaatttt tcccgctcgg gggaaaccag accgcaaaag agtccgaagc gagtgttgtt
     9061 cgccatccga atcgcctcat caaaagtatc ataacgccag acgcgcaaaa acggcccgaa
     9121 cacctcttca tccggcacac cgccaacgcc cgtcatttca atgatccctg gtgtcagcaa
     9181 cgatgtccct gcttgtaata agcgcggcgc aagcagggtt cgtccgccca tcgcttccag
     9241 ttgctgccag gcagtaacca cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa
     9301 cggctgcggt tcgtcatccc agttgcccgg cgttaatcgc tggctgacgg caaccagact
     9361 agcaagaaac gcatcgccct gcgctccgct tttgagcaat aaacggcggg cgcaggtgca
     9421 gcgttgcccg gctgtgacaa acgccgactg aatggtcaga tggacagccg cgtcgatatc
     9481 cgccacctca tcgataatta gcggattatt accgcccatc tcaagggcga gaattttctc
     9541 cggctgaccg gagagctggc gatgcagctg gtagccggta ttggcgctac cggtaaacag
     9601 caaaccgtcg agatcctcca gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac
     9661 caggttcagc acgcccggcg gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc
     9721 actccacggt gtcagttcgc tgggtttaaa gataatggtg ttacctgcca gcaatgccgg
     9781 aacgatatgt ccgttcggca aatgaccagg gaaattatac ggcccaaaca ccgccagcac
     9841 gccgtgcggg cgatgtcgca ggctcgccgc accgtccggc atttcactac gctgctcgcc
     9901 ggtacgaacg tgatacgcct taattgatat cgcgatttta ttgatcatcg ccgtcacttc
     9961 ggttgccgct tcccagcgcg gcttacccgt ttctctggca ataatcgcgg ttaattcggc
    10021 tttattgctt tccagcaatc cggcaaagcg ttcgacaacg acctgacgtt cagccaatga
    10081 gagccgcgcc cagcgcggaa acgccgcacg ggctgcccga caagcctgtt cgacctgagc
    10141 ggcatcggca tcattgcctt gccataacac ctcgcccgat accggattac gcttcacacg
    10201 cgatgcgccc tggcccgtta tccagtcacc gttaatccat aaagtcatgc tgttttctcc
    10261 tctgcgcaca ggcgcaccag acgaacgcga tcccctgcgt ggcatttgag ggcatccagt
    10321 tgtgcggcgg ttaaaatcaa acgctcggtt gccggatcgg tacgcgccag caccacgcgg
    10381 aaatggtgat aattttcatt ggcgaccagg caggccggaa aatcgccctg cgcaggctgc
    10441 ccttctgcca cttccaccag ccgactttta cggatggcgc gcacgcggtc gatgtcacac
    10501 tcaagcgttg gcccgccgtc gaagatgtcg atatagttgc ggtaacgaaa accttctttc
    10561 tccagcaccg cgcgggcagg cgcggtttgc ggatgtacct gaccgatgac gtcctgggct
    10621 tcctgggata aaaagtgggt atagatcgga tgtttcggca tcagttctgc aataaatgcc
    10681 ttttgcccgg tgccgcagag aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta
    10741 ccgaggcttt gccagaacgg tgaatagccg tgttcgtcaa tcaccccgcg catttcagca
    10801 accactttgt cattaaactt gtcgcgaaaa gccgccataa acataaagcg cgatttcgac
    10861 agcaaatagc cgttgccctc tttgcgccag tccgggtcga gaaacagcgt gcacagctcg
    10921 ctgctgccgg tgtgatcgtt actaagaaac agcgtcggca atgcgttata gacattcagc
    10981 tcttttgagg cgtgaaccaa cgtgccgacg cgatagttgt accagggatc gttcagccca
    11041 accgccacct caatagcgca aatccccgcc acggtgcctg tctcgctatc ttccagcacg
    11101 aacacatagc cctgctcgct tttgggcagt tcaccttgcc aggttttgat tgccctttcg
    11161 atacgcgccg aaagcgtggc ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg
    11221 ctggcaagct gcatcagcgc cgagacatct gagcgctcaa cgggacggat gaccatcatg
    11281 atgaacctcg gctaacaaag tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca
    11341 cctcttcttc gctgacattg agcgcaggcg caaaacgcac cacgttgcca cccgcaatca
    11401 gtaccatcac gcctgctttc gccgcttcca gagagatctg tttcgcttgc ccggcgtaat
    11461 cggcattcag tacacagcca atcaacaaac ctaagccgcg aatttcactg aacaaaccat
    11521 agcggtgatt aatagtatta agacgctcaa caaaccagtc gtgacgctgt ttaacgccat
    11581 taagcatctc tggtgtgttg atgagctcca gcactttgcc tgctaccgcc gaggccagcg
    11641 gattaccgcc ataggtggtg ccatgagtgc caacggtcat cacgcgggcg cactcttcgg
    11701 ttgccaacaa cgcaccgacc gggaaaccgc cgcccagcgc tttggcggtg gttaacagat
    11761 cgggcgtcac gccgtagtgc atataggcat acagctcacc agtgcgcccg acgccggttt
    11821 gtacctcatc aaaaatcagc aaggcattgt ggcggtcaca caattcacgc agaccttgta
    11881 aaaacgcgtt gctggctggc accacaccgc cttcgcccta gatgggctcg acaatcaccg
    11941 cacaggtggc gtcgtcaatc agcgcgctgg cagagttaat atcgttatat gcagcatgac
    12001 gaatatccgc cggcagtggc gcaaaatcct gtgaataggc tggctgccca cccgcactga
    12061 cagtaaacag cgtgcgacca tgaaacgcat ttttgaacgc cacgatgcca ctcttatggc
    12121 tgccgtagcg gtcgtgagcg aatttacgcg ccagttttag cgccgcttcg ttggcttccg
    12181 caccggagtt acaaaagaag acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca
    12241 gtcgcagtac cggctcgttg gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt
    12301 cgttcagcgc ttcacgcagt tccggatgcg catggcccag cgcgttcacc gcaatgccac
    12361 ccgcgaagtc gatatactct ttcccctgct gatcccacaa gcgcgaacct tcgccacgta
    12421 ccggtataaa gggtgccgga gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg
    12481 taattggctg agacatagcg acctctacag taaataattc gttatttgta tgttaacaat
    12541 aagtaatgtt tgcgctgtaa atgtagattg cagggttcgt gccagccagt gataaaagtg
    12601 cataaacggc ggaggctaac tggaaatcaa ggagttataa ccaaaccata tgcatttaaa
    12661 gtgcatataa agtgaatacg tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg
    12721 ttatgcagaa gtaaaatata attctagaat tgtgatcatt gacgaaattt actggaaatt
    12781 actgcgccat tctgacgcag cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca
    12841 tcattaacaa ccatcgatca aatcacttaa caacaggcgg taagcaacgc gaaattctgc
    12901 taccatccac gcactcttta tctgaataaa tggcagcgac tatgaaattt gtctctttta
    12961 atatcaacgg cctgcgcgcc agacctcacc agcttgaagc catcgtcgaa aagcaccaac
    13021 cggatgtgat tggcctgcag gagacaaaag ttcatgacga tatgtttccg ctcgaagagg
    13081 tggcgaagct cggctacaac gtgttttatc acgggcagaa aggccattat ggcgtggcgc
    13141 tgctgaccaa agagacgccg attgccgtgc gtcgcggctt tcccggtgac gacgaagagg
    13201 cgcagcggcg gattattatg gcggaaatcc cctcactgct gggtaatgtc accgtgatca
    13261 acggttactt cccgcagggt gaaagccgcg accatccgat aaaattcccg gcaaaagcgc
    13321 agttttatca gaatctgcaa aactacctgg aaaccgaact caaacgtgat aatccggtac
    13381 tgattatggg cgatatgaat atcagcccta cagatctgga tatcggcatt ggcgaagaaa
    13441 accgtaagcg ctggctgcgt accggtaaat gctctttcct gccggaagag cgcgaatgga
    13501 tggacaggct gatgagctgg ggggtggtcg ataccttccg ccatgctaat ccgcaaacag
    13561 cagatcgttt ctcatggttt gattaccgct caaaaggttt tgacgataac cgtggtctgc
    13621 gcatcgacct gctgctcgcc agccaaccgc tggcagaatg ttgcgtagaa accggcatcg
    13681 actatgaaat tcgcagcatg gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc
    13741 gccgctaatt tagcagctct cctggctcaa actgggtcag gagaattaac cttgagaaaa
    13801 atcaacaaac tgtcagtaat gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt
    13861 ttttagttgt ctgaccactt ctctattatc aagtttgata taggaaactc cacgatgaac
    13921 gctgagcgta actttctgtt tgcctgtctt atttttgcgc tggtcattta cgctatccac
    13981 gctttcggtt tattcgatct gctcaccgat ttaccccact tacagacact cattcgccag
    14041 agcggattgt tcggttatag cctctatatt ctgttattca tcaatgcccc cctctttctg
    14101 ttaccaggaa gcatactggt gatcgctgga ggaatagttt ttggcccgct cttagggaca
    14161 caactctcat taattgccgc aacgctggcc tcctcgtgct cattcctgct ggcgcgctgg
    14221 ctgggacgtg atttactgct gaaatacgtg ggccatagcc ataccttcca ggccattgaa
    14281 aaaggcattg cgcgtaacgg tattgatttt cttattctga cccgcttaat cccgttgttt
    14341 ccttacaata ttcaaaatta cgcttacgga ttcaccacca tcgccttctg gccttatacc
    14401 cttatttcgg cactcacgac cctgcccggt attgttattt ataccgtgat ggcaagcgat
    14461 ctcgccaatg aaggcattac gctgcgcttt attttacaac tctgtctggc gggcctggcg
    14521 ctgtttattc tcatccagct cgcaaaactc tacgcccgac acaaacatgt ggatctgtct
    14581 gcttcgcgcc gcagcccact tactcaccca aaaaatgaag gatagaacga tgtcgcaaca
    14641 ttattcagtg tcatggaaaa aaggactggc tgcactctgc ttactggctg ttgccgggct
    14701 tagcggctgc gatcaaaaag agaatgcggc ggcaaaagtg gaatacgacg gactttcgaa
    14761 cagccaaccg ctgcgcgtcg atgccaataa ccatacggta accatgctgg tgcaaattaa
    14821 tggtcgtttc ctcaccgacg acactcgtca cggtattgtg tttaaagatg gctccaacgg
    14881 acataaatcg ctgtttatgg cttatgcgcc cccgaaagca ttttatgaag ccctgaaaga
    14941 ggcaggtggt acgccgggcg aaaacatgac gatggataat aaagaaacga ctcatgtcac
    15001 aggcagcaaa ctggatattt cggtcaactg gcaaggggcg gcaaaagcgt attccttcga
    15061 tgaagtgatt gttgacagta atggcaagaa actggacatg cgctttggcg gtaatttaac
    15121 ggcagcagaa gagaagaaaa cgggttgcct ggtgtgtctg gatagctgcc cggtcggcat
    15181 cgtcagcaat gcaacataca cttatggtgc ggtggaaaaa cacggcgaag tgaaattcaa
    15241 aggcaatgcc tcagttctcc cggcggataa cacgctggca acggttacct ttaaaatcac
    15301 cgaataaagc caagataaag gatgatgaag atacaatcgc gtaaaatctg gtactaccgt
    15361 ataaccctca tcatcctgtt gttcgctatg ctgctcgcat gggcgctgct tcccggcgtc
    15421 catgagttta tcaatcgcag cgttgcggcg tttgccgccg tggaccaaca gggtatagaa
    15481 cgctttattc agtcttacgg cgcactggcg gcagttgtct cgttcttgtt gatgatttta
    15541 caggccattg ctgcaccgct acctgcgttt ttgatcacct ttgccaatgc gtcgctgttt
    15601 ggcgcgttct ggggcggctt gctgtcgtgg accagttcga tggccggcgc ggcgctgtgc
    15661 ttttttatcg ccagagtgat gggccgcgaa gtggtggaaa aattaaccgg caaaaccgtg
    15721 cttgacagta tggacggctt tttcactcgc tacggcaaac acaccatcct ggtctgtcgg
    15781 ttattgcctt ttgtcccttt cgatccaatc agctatgctg ccggtctgac ttcaatacgt
    15841 tttcgctcgt tttttatcgc caccgggctt ggtcaattac cggcgactat tgtttattcc
    15901 tgggcaggca gcatgttaac aggcggtact ttctggtttg tcaccgaact gtttattctg
    15961 tttgccctga ccgtggtgat ttttatggcg aagaaaatat ggcttgagcg ccagaagagg
    16021 aatgactgat gggtttaccg ccgcttagca aaattccttt cattttacgt ccgcaggcgt
    16081 ggctgcatcg tcgccattac ggcgaggtgc taagcccgat tcgctggtgg gggcggatcc
    16141 cgtttatctt ttatttggtg tcaatgtttg ttgggtggct ggagcgcaaa cgctcaccgc
    16201 tcgatccggt agtgcgatcg cttgtcagcg cgcgcattgc gcaaatgtgc ctgtgtgagt
    16261 tttgcgtgga tatcaccagt atgaaagtcg ccgagcgtac cggcagcacc gataaa
//
LOCUS       DAJTRH010000084        15635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTRH010000084 DAJTRH010000000
VERSION     DAJTRH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            156..1214
                     /gene="citC"
                     /locus_tag="OO339_002971"
     CDS             156..1214
                     /gene="citC"
                     /locus_tag="OO339_002971"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5927522.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1229..1525
                     /gene="citD"
                     /locus_tag="OO339_002972"
     CDS             1229..1525
                     /gene="citD"
                     /locus_tag="OO339_002972"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5927523.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1522..2430
                     /gene="citE"
                     /locus_tag="OO339_002973"
     CDS             1522..2430
                     /gene="citE"
                     /locus_tag="OO339_002973"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5927524.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2441..3973
                     /gene="citF"
                     /locus_tag="OO339_002974"
     CDS             2441..3973
                     /gene="citF"
                     /locus_tag="OO339_002974"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5927525.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3977..4527
                     /gene="citX"
                     /locus_tag="OO339_002975"
                     /pseudo
     CDS             3977..4527
                     /gene="citX"
                     /locus_tag="OO339_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4502..5380
                     /gene="citG"
                     /locus_tag="OO339_002976"
     CDS             4502..5380
                     /gene="citG"
                     /locus_tag="OO339_002976"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5927526.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5431..6893
                     /gene="citT"
                     /locus_tag="OO339_002977"
                     /pseudo
     CDS             5431..6893
                     /gene="citT"
                     /locus_tag="OO339_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7007..7813
                     /gene="rna"
                     /locus_tag="OO339_002978"
     CDS             7007..7813
                     /gene="rna"
                     /locus_tag="OO339_002978"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5927527.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8042..8452
                     /gene="rnk"
                     /locus_tag="OO339_002979"
     CDS             8042..8452
                     /gene="rnk"
                     /locus_tag="OO339_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5927528.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8683..9892)
                     /locus_tag="OO339_002980"
                     /pseudo
     CDS             complement(8683..9892)
                     /locus_tag="OO339_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            10113..10541
                     /gene="uspG"
                     /locus_tag="OO339_002981"
     CDS             10113..10541
                     /gene="uspG"
                     /locus_tag="OO339_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5927529.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10662..12227)
                     /gene="ahpF"
                     /locus_tag="OO339_002982"
     CDS             complement(10662..12227)
                     /gene="ahpF"
                     /locus_tag="OO339_002982"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5927530.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12472..13035)
                     /gene="ahpC"
                     /locus_tag="OO339_002983"
     CDS             complement(12472..13035)
                     /gene="ahpC"
                     /locus_tag="OO339_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5927531.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13407..14153
                     /gene="dsbG"
                     /locus_tag="OO339_002984"
     CDS             13407..14153
                     /gene="dsbG"
                     /locus_tag="OO339_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5927532.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14363..15265
                     /locus_tag="OO339_002985"
     CDS             14363..15265
                     /locus_tag="OO339_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5927533.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15412..15615
                     /locus_tag="OO339_002986"
                     /pseudo
     CDS             15412..15615
                     /locus_tag="OO339_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3848 a         4061 c         3812 g         3914 t
ORIGIN      
        1 gaaatactcc ttaggatgta tagcaaaagg agaaaaagat atacctcgat cacccccctt
       61 tctcccaagt gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat
      121 cgataacaac atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc
      181 gcgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg
      241 atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta
      301 tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc
      361 gcggtgaagg actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc
      421 acagcacgca tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg
      481 gtttttccac gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc
      541 gactgaaacg ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct
      601 gcattgtgat gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg
      661 cggcacagtg cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct
      721 atgaagaccg gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc
      781 atcgcggctc agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac
      841 agagcgtcat taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg
      901 ctcccgcgct tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg
      961 cccagtacaa ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca
     1021 tcgaactggt tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg
     1081 tacgtcaact gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca
     1141 cgctgcatta tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa
     1201 agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac
     1261 ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct
     1321 gcaaatcaat agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga
     1381 cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact
     1441 ggactgcatt ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc
     1501 tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc
     1561 cgccgcagca tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc
     1621 tacccggctg atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac
     1681 accgcccgcc gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc
     1741 attgtgcgtg tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt
     1801 cgcggtggtg cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat
     1861 attgaaaaag agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc
     1921 ctgctggcgg cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct
     1981 tccgagcgtt tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca
     2041 gaacgctccc cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg
     2101 cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga
     2161 ttcctgcaag aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac
     2221 ccgcgtcaga ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac
     2281 gcccaccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc
     2341 ctgaacggca agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt
     2401 gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca
     2461 atctcaacga caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc
     2521 tttccagaac tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg
     2581 cgccaacctg gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt
     2641 ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa
     2701 gatgggcttt aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct
     2761 ggtagaacac attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc
     2821 actggcggaa gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg
     2881 cggtcgtgtg catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt
     2941 cccgtcctgt gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc
     3001 cctcggctat gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga
     3061 actgctgcct tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt
     3121 caaagttaat cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac
     3181 taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta
     3241 cttcaaagaa ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg
     3301 tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat
     3361 taccgcgacg atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca
     3421 gagctttgac agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag
     3481 cgccaaccag tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt
     3541 ggtactgagc gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga
     3601 cggcgtactg cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc
     3661 catcatcgtc gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac
     3721 ctgcatcacc ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa
     3781 cccggcacgt ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat
     3841 tgagtggctg cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac
     3901 cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca
     3961 ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat
     4021 tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca
     4081 ccatcctgtt ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga
     4141 ggtcacacgc cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca
     4201 gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt
     4261 gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca
     4321 tcctctcggg cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg
     4381 ccgcgactat tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc
     4441 gcgcgtggaa aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac
     4501 gatgtcgatg cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga
     4561 gtacgccctg ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg
     4621 cctcgtggat cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg
     4681 cagcgcgctg gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc
     4741 agaaatggca ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg
     4801 tgatatgttc cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg
     4861 gctgctatgt gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac
     4921 cgtttgttct acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa
     4981 taattcacga ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg
     5041 cggtgaagcc gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac
     5101 tctgctggat caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat
     5161 ggctaccaac ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct
     5221 acagcgcgag gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga
     5281 ttatctccgg cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc
     5341 cgacctgctg atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa
     5401 tttggaaata ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa
     5461 ctattggccc cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg
     5521 ccgccgcagg catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc
     5581 gagccaattc cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt
     5641 aattacctgc tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag
     5701 gcgctgaaat ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca
     5761 tttatttttg cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg
     5821 gtgaaattca tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt
     5881 ctgctggcac cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc
     5941 attaaaaacc tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt
     6001 ggcggctatt tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc
     6061 accggtgcgg accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga
     6121 ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg
     6181 cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa
     6241 cctgggcggg tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga
     6301 ttggccttgt attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta
     6361 ctgcggttgg tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag
     6421 acattacccg ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga
     6481 tggctaacgg cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc
     6541 acctggaagg attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg
     6601 cacactacct gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg
     6661 ccgtcggtaa aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt
     6721 ctatcggtat catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg
     6781 gctgtggcta tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt
     6841 acatctctat gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc
     6901 gccaaacgct gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat
     6961 gatttaatag atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg
     7021 ccgtaacgcc gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc
     7081 gttgcaggca aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac
     7141 cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca
     7201 aaccgaaacg accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc
     7261 taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac
     7321 tcgcccaatc ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac
     7381 cgggttatca ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg
     7441 ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga
     7501 cgcatacttc ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa
     7561 attccttgcg gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc
     7621 caaaagctgg ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc
     7681 gtatttgact gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc
     7741 aaactcattc ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa
     7801 agcgggttat taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg
     7861 attaagaatt atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact
     7921 tcaaagagtg agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg
     7981 agggttttct tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa
     8041 aatgtccaga ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct
     8101 ggagcaaccc gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg
     8161 cgcccaaatg tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt
     8221 taaattccgc aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa
     8281 aatgaccgat agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg
     8341 gctgcgcgtt ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga
     8401 agtgctggaa ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct
     8461 gcacaagcga taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca
     8521 gcaatttttc ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg
     8581 ggaataacca tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt
     8641 atcgtcatgg ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg
     8701 catcgcattc accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg
     8761 taccagaatc accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc
     8821 ggcctcttca aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc
     8881 aattaacggt aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt
     8941 caacccttta tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc
     9001 cggtacgcta acaatgccgc aacgctttac cgctgctgcc ctccagtttc aggttagtca
     9061 gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac ggcgtctatt actgcatcca
     9121 cgccccggtg acctgccgtt tgttcaataa ttgactgtgc cggatcgcta tcttcatcga
     9181 aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg caagcggtag ggatgatgat
     9241 caacaacaaa aatctgttcc gcaccgagca accgtgcaca ggcgattgtc aacaatccca
     9301 caggaccagc accatagact gcaacgcttg aaccttgttg gatctgcaca ttttttgctg
     9361 cctgccatgc cgttggcaga atatcagaaa ggaaaagggc tttatcatct gaaagcaaag
     9421 gcggtacttt aaacggcccc acattccctt tcgggacgcg gacatattcc gcctgcccgc
     9481 caggaacgcc accatacagg tgactataac caaacaatgc cgcgggagca ggtatctgtt
     9541 ttttattgag cgcagcgcct ttacccgcat tggtattttc gcaggcggca tattgctgta
     9601 atcgacagaa aaaacagtcg ccacaagcaa tgacgaacgg aattaccacc cggtcgcctt
     9661 tttgcaaatt ttttacgtcc ttcccggttt caacgacttc ccccataaat tcatgaccaa
     9721 aaatatcgcc atgtttaacc tggggtattt tgcctcgata aagatggagg tcagagccac
     9781 agatcgccgt taccgtaata cgcagaataa tatcatctgc ctgttcaatg cccggatcgg
     9841 gaacattttc tacctgaacg tgatgtgggc cgtgataagt caatgctttc atgcaacctc
     9901 cagagacagt tcaaaaaaga gtcagtcttt aagggtagct atcacagcgg gaaaacgggc
     9961 ggtaccgcag gttttgggag aattcctagt gttcagtgag aatatattaa tcacagaaac
    10021 cgttcgtaag gaaaaagaac cagacaaata tcagggataa atattataat catggagaat
    10081 gatttgttca tgattaacag ggagaaaggg ttatgtataa ggcaatcatt atgccagttg
    10141 atgtatttga aatggaattg agcgacaaag ctgttcgcca cgctgaattc ctcgcccagg
    10201 atgacggagt tattcatcta cttcacgtac tacccgggtc agccagcctg agcctgcacc
    10261 gttttgccgc tgatgtgcgt cgttttgaag agcatctgca acatgaagca gaagaacgtc
    10321 tgcaaacgat ggtcagccac ttcaccatcg atccttcccg cattaaacaa catgtccgtt
    10381 ttggtagcgt gcgggatgaa gtcaatgagt tggcagaaga actgggggct gatgttgtag
    10441 ttatcggttc tcgcaaccca tcgatttcga cccatctgtt aggttctaac gcctcgagcg
    10501 tgatccgcca cgccaatctg cctgtactgg tcgttcgcta acagataaaa gtatttatgc
    10561 cgccctgcct ggaacatccc ggcagggctt tttaatgcca taaaaaagcc gccaggtttg
    10621 accccggcgg ctaagcaatt gcaggtgaat cttacttctt cttatgcagt tttggtgcga
    10681 atcaggtagt caaaagcact cagagaggct ttggcacctt cgccagtggc gatgatgatc
    10741 tgcttgtacg gaaccgtcgt acagtcaccc gctgcgaaca cgcctttgac gttggtttca
    10801 catttcgcat caatgataat ctcgcccatg cggttacgtt cgactgcgcc ttccagccag
    10861 ttggtgttcg gcagcagacc aatctggaca aaaataccgg ccagttcgat gttgtgaata
    10921 ttgccgctga cacgatctcg atattccaga ccaacgactt tgctgccgtc gcctttcact
    10981 tccgtggttt gcgcattcag aataatgtcg acgtttttca ggctgcgcag tttgtcctgc
    11041 agaacctggt cggctttcat ttctggcgca aattccagca gcgttacgtg ctcaacgata
    11101 cccgccaggt cgattgccgc ttccacgccg gagttaccgc cgccgataac cgctacgcgt
    11161 ttgcctttaa acagcgggcc gtcgcagtgc gggcagtagg tcacgccttt agtgcgatac
    11221 tgatcttcgc ccggaacgtt catgttgcgc cattttgcac ccgtcgccac gataatgctg
    11281 cgtgctttca gtaccgcgcc agaagctgtt tcaatctggt gtagaccacc ttcaaccgct
    11341 gccgggatca gtttgctggc gctctggctg tcgatcacat caacgtcgta ttcatcaacg
    11401 tgaactttca gtgcgcctgc caacttctgc ccttcagtct tcggtacaga aatgtagttt
    11461 tcgatatcaa cggtatcgag gatctgacca ccaaaacgtt cgcccatcag accggtacgg
    11521 atgcctttac gtgcggagta aattgctgcc gctgcacccg ccgggccgga accaacgatt
    11581 aatacgtcat aagcatcacg cttgttcagc tcttctgccg cacgattttc cgcgccagta
    11641 tcaattttgg caacgatttc agtcaacgtc atgcggccct gaccaaactc tttcccgttt
    11701 acgaacactg ccggaacgcc catcacgttg cgatcggtga tttcgttctg gaaggtgccg
    11761 ccgtcaattg cagtgtgctt gatgcgcggg ttcaatacgc tcatcaggtt cagcgcctgc
    11821 accacgtccg ggcagttatg gcaagagagc gagtaatagg tttcgaattc aaaatcaccg
    11881 tcaatatggc gaatctgctc cagcagagac tgcgcttctt tcgacggatg accaccggtc
    11941 cacagcaacg ccagtaccag cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt
    12001 ggtccctggt tggaacctgg gttggtgatc aggaaagacg gcttacgcac tggcaagctg
    12061 ttatcttctt taaaggtgac tttgtctgac agttctgcga tttcagccaa cagttccttg
    12121 atttctgccg atttagcgct gtcatccagc gtggcaatta actcaacagg cttggtcaat
    12181 ttctcaaggt aagccttgag ttgagttttc atatttgtgt cgagcatgtt tatctcctgg
    12241 gctttaaaca tcatcatgca agcggcctct gcggctacct ggatgcagct tgcatcatgg
    12301 tgcggggaag aaaatcgggt gccgcagcac ccgaagaatt aggtgaattt ccgaagtatt
    12361 tcgcgtcaga gcaaggcgac tagcctgtga atccccggga gcttacacaa gtaagtgacc
    12421 ggggtgagcg ggcgacgcca acgccgctat ggcgtgaaag acgaaggaaa tttagatttt
    12481 accaaccagg tccagagacg gagccagagt tgcttcacct tctttccatt tagccgggca
    12541 aacttcacct gggtgagaag ctacgtactg agctgctttg attttacgca gcagatcaga
    12601 cgcgtcacgg ccaatgcctt cagcggtaac ttcgattgcc tggatgatac cctgcgggtc
    12661 aacaacgaag gtcgcacggt cagccagacc ttcatcttca cgcatgttgt cgaagttacg
    12721 ggtcagggcg ccagtcgggt cgccgatcat cgcatatttg attttagcga tggtttcaga
    12781 gctgctgtgc catgctttgt gggtgaagtg agtatcggta gatactgcgt atacgtctac
    12841 gcccagtttc tgcagttctt cgtagtggtc agcaacgtca cccagttcgg tcgggcatac
    12901 gaaagtaaag tcagccgggt agaagaagaa gacgctccag cggccttcgg tatctttttc
    12961 ggtgatttcg atgaattcgc cgtttttgaa tgcctggttt ttaaaaggtt taattttggt
    13021 gttaatcaag gacatctata cttcctccgt gttttcgatg agatgtaagg taaccaattt
    13081 ttgccgattc ggctaatgcg tttccattat caaatcgata agttttacct tacaaccttc
    13141 gtaagacaac tttgtgaact ttgcaccgcc agcggcgata aaaagtaaaa tggccccctt
    13201 tccaggtgac cgtgtaatct gaactaccct ttggtatctt cagcgcctgc ctgcaggctg
    13261 aggtgttgcc cttacaaatg caacaacgac atggattaca acaccctcat aaacaaaggg
    13321 caatcacctg atctaagctc ttacctatga cagtgatagg ttatgccttt tactcgactt
    13381 ttgcactgac tgaaaagaac aaattaatgt taaaaaagat acttttactg gctctgcttc
    13441 ctgcaatcgc cttcgcagag gaacttcctt ccccagtaaa agcgattgaa aaacagggca
    13501 ttacaatcat caaaacgttc gatgcccccg gaggaatgaa aggttatctc ggaaagtatc
    13561 aggatatggg cgtcaccatc tacctgactc ccgatggtaa gcacgctatc tctggttaca
    13621 tgtacaacga gaaaggtgaa aacctgagta acacacttat cgaaaaagaa atttacgcac
    13681 cagccggacg cgaaatgtgg caacggatgg aacaatccca ctggctcctc gacggtaaaa
    13741 aagatgcgcc ggtcattgtc tacgtcttct ccgatccgtt ctgcccatat tgtaaacagt
    13801 tctggcagca ggcgcgcccg tgggtagatt ctggcaaagt gcaattaaga acattgttgg
    13861 ttggggttat caagccagaa agcccggcga cagcagcggc aattcttgcc tccaaagatc
    13921 ccgcaaaaac ctggcaacaa tatgaagcct ctggttgcaa gcttaagcta aacgtgcctg
    13981 caaacgtaag tacagagcaa atgaaagtgt taagtgacaa tgagaaactg atggacgatc
    14041 tgggggcaaa tgtcacgccg gctatctatt acatgagtaa ggaaaatacg ctgcaacagg
    14101 ccgtggggtt gcccgatcag aaaacgctta atatcattat gggaaataaa taaaaggtaa
    14161 ttcatggatg gttgaactat atccctgctt ttcaaccatc ctaaaaaagc atgaatttct
    14221 cgctctaccc taagtggctg tgctatttta gaaaaactta atcctgagtt aagcaaaaaa
    14281 acataatcaa taaaaataca tacgcaaaaa acattgatta agtgaatata ccatggaaga
    14341 aaaatataaa ccggagtagt gtatggccaa tctctacgac ttgaaaaagt tcgacttaaa
    14401 tcttcttgtc atatttgagt gtatttacca gcatttaagc attagcaaag ctgctgaatc
    14461 actatatatc accccatcag ccgtgagcca gtcgttacaa cgcttacgtg cgcaatttaa
    14521 tgacccttta ttcattcgct caggaaaagg tatcgcgccg acgacaacag ggttgaatct
    14581 gcatcaccat cttgagaaaa acctcaaagg ccttgaacaa acaattaata tagtcaataa
    14641 gtccgaacta aagaaaaact ttattatcta cggccctcag ctcatttctt gctctaataa
    14701 tagtatgctg atccgctgcc tacggcaaga tgcctctgtc gagattgagt gccatgatat
    14761 cctcatgtca gcagagaatg ctgaagaact gttggttcac cgcaaagccg atctggtgat
    14821 cacccaaatg ccagtgatca gccgttccgt catttgcatg ccattgcata caattcgcaa
    14881 tactctaatt tgtagtaata agcatccgcg aattactgac aatagcactt atgaacaaat
    14941 tatggcagaa gagttcaccc aattaatagc caaatcagca ggagttgatg atattcaaat
    15001 ggaaatagac gagaagttta tgaatcgcaa aatttcattt cgtggttcct ctttgctgac
    15061 aataattaat agcattgcag ttacagattt actgggaata gtcccctatg aactttataa
    15121 ctaccaccgt gactttctta atttaaaaga gataaaacta gaacatccgt taccttcaat
    15181 aaagctttac atttcctata ataaatcttc tttaaataat ttggtgttct cccgatttat
    15241 tgaccgtcta aatgagagtt tttgatatat aactatagac agctactata actttcatct
    15301 atttattcac agcgcgaatg aaaaataaaa cactctgctt tacagtgttt actttataat
    15361 taacagtctt atctcactgt tcaccctgta acgttcagag agcgtcttca gatgtctatt
    15421 tataaaattc ctcttccgct caatattctg gaagctgcac gagagcgtat tacctagaca
    15481 ctcaacaccc taccccgcgt ttgtgtctca ttttctggcg gcaaagactc tggtttaatg
    15541 ctgcatctga cagccgaact tgcccgacaa atgggcaaaa aaatctgcgt tttgtttatc
    15601 aactgggagg cgcaatgaat cgccacggat aatct
//
LOCUS       DAJTRH010000085        15581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTRH010000085 DAJTRH010000000
VERSION     DAJTRH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..74)
                     /locus_tag="OO339_002987"
     CDS             complement(<1..74)
                     /locus_tag="OO339_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5927534.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="OO339_002988"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="OO339_002988"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5927535.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="OO339_002989"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="OO339_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5927536.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="OO339_002990"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="OO339_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5927537.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="OO339_002991"
     CDS             2704..2940
                     /locus_tag="OO339_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5927538.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="OO339_002992"
     CDS             2943..3800
                     /locus_tag="OO339_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5927539.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="OO339_002993"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="OO339_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="OO339_002994"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="OO339_002994"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5927540.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="OO339_002995"
     CDS             complement(6415..7311)
                     /locus_tag="OO339_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5927541.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="OO339_002996"
     CDS             complement(7360..7599)
                     /locus_tag="OO339_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5927542.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="OO339_002997"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="OO339_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5927543.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="OO339_002998"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="OO339_002998"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5927544.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="OO339_002999"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="OO339_002999"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5927545.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="OO339_003000"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="OO339_003000"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5927546.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..12880
                     /locus_tag="OO339_003001"
     CDS             10766..12880
                     /locus_tag="OO339_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5927547.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12890..14182
                     /locus_tag="OO339_003002"
     CDS             12890..14182
                     /locus_tag="OO339_003002"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927548.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15132..15224
                     /locus_tag="OO339_003003"
     CDS             15132..15224
                     /locus_tag="OO339_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5927549.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15268..>15581)
                     /locus_tag="OO339_003004"
     CDS             complement(15268..>15581)
                     /locus_tag="OO339_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5927550.1"
                     /translation="LDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKE
                     NRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQAT
                     A"
BASE COUNT         3572 a         4127 c         4092 g         3790 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt a
//
LOCUS       DAJTRH010000086        15526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTRH010000086 DAJTRH010000000
VERSION     DAJTRH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO339_003005"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO339_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5927551.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO339_003006"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO339_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5927552.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO339_003007"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO339_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO339_003008"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO339_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5927553.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO339_003009"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO339_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5927554.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO339_003010"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO339_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5927555.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO339_003011"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO339_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5927556.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO339_003012"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO339_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5927557.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO339_003013"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO339_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5927558.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO339_003014"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO339_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5927559.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO339_003015"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO339_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5927560.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO339_003016"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO339_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5927561.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO339_003017"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO339_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5927562.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO339_003018"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO339_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5927563.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO339_003019"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO339_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5927564.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO339_003020"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO339_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5927565.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO339_003021"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO339_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5927566.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO339_003022"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO339_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5927567.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO339_003023"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO339_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5927568.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO339_003024"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO339_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5927569.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO339_003025"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO339_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5927570.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO339_003026"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO339_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5927571.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO339_003027"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO339_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5927572.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO339_003028"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO339_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5927573.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO339_003029"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO339_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5927574.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO339_003030"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO339_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5927575.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO339_003031"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO339_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5927576.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO339_003032"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO339_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5927577.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO339_003033"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO339_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5927578.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO339_003034"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO339_003034"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5927579.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OO339_003035"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OO339_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5927580.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3643 c         4130 g         3746 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAJTRH010000087        15344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTRH010000087 DAJTRH010000000
VERSION     DAJTRH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO339_003036"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO339_003036"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5927581.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO339_003037"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO339_003037"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5927582.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO339_003038"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO339_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5927583.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OO339_003039"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OO339_003039"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5927584.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OO339_003040"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OO339_003040"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5927585.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OO339_003041"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OO339_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5927586.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OO339_003042"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OO339_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5927587.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OO339_003043"
     CDS             complement(8064..9206)
                     /locus_tag="OO339_003043"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5927588.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OO339_003044"
     CDS             complement(9215..10228)
                     /locus_tag="OO339_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5927589.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO339_003045"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO339_003045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5927590.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO339_003046"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO339_003046"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5927591.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO339_003047"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO339_003047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5927592.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OO339_003048"
     CDS             complement(12826..13950)
                     /locus_tag="OO339_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5927593.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO339_003049"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO339_003049"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5927594.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4381 a         3444 c         3145 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aatatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaaa
//
LOCUS       DAJTRH010000088        15170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTRH010000088 DAJTRH010000000
VERSION     DAJTRH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..935)
                     /locus_tag="OO339_003050"
     CDS             complement(<1..935)
                     /locus_tag="OO339_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5927595.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVG"
     gene            complement(1134..1218)
                     /locus_tag="OO339_003051"
     tRNA            complement(1134..1218)
                     /locus_tag="OO339_003051"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1182..1184),aa:Leu,seq:caa)
     gene            1414..2433
                     /gene="ahr"
                     /locus_tag="OO339_003052"
     CDS             1414..2433
                     /gene="ahr"
                     /locus_tag="OO339_003052"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5927596.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2562..4064
                     /locus_tag="OO339_003053"
     CDS             2562..4064
                     /locus_tag="OO339_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5927597.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4183..5265)
                     /gene="lptG"
                     /locus_tag="OO339_003054"
     CDS             complement(4183..5265)
                     /gene="lptG"
                     /locus_tag="OO339_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5927598.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5265..6365)
                     /gene="lptF"
                     /locus_tag="OO339_003055"
     CDS             complement(5265..6365)
                     /gene="lptF"
                     /locus_tag="OO339_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5927599.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6632..8143
                     /gene="pepA"
                     /locus_tag="OO339_003056"
     CDS             6632..8143
                     /gene="pepA"
                     /locus_tag="OO339_003056"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5927600.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8277..8720
                     /gene="holC"
                     /locus_tag="OO339_003057"
     CDS             8277..8720
                     /gene="holC"
                     /locus_tag="OO339_003057"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5927601.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8720..11575
                     /gene="valS"
                     /locus_tag="OO339_003058"
     CDS             8720..11575
                     /gene="valS"
                     /locus_tag="OO339_003058"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5927602.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11629..12825)
                     /locus_tag="OO339_003059"
     CDS             complement(11629..12825)
                     /locus_tag="OO339_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5927603.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13018..13521
                     /locus_tag="OO339_003060"
     CDS             13018..13521
                     /locus_tag="OO339_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5927604.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13567..13983)
                     /gene="rraB"
                     /locus_tag="OO339_003061"
     CDS             complement(13567..13983)
                     /gene="rraB"
                     /locus_tag="OO339_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5927605.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14145..15158
                     /gene="argF"
                     /locus_tag="OO339_003062"
     CDS             14145..15158
                     /gene="argF"
                     /locus_tag="OO339_003062"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5927606.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3921 a         3846 c         3926 g         3477 t
ORIGIN      
        1 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
       61 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      121 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      181 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      241 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      301 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      361 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      421 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      481 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      541 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      601 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      661 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      721 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
      781 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
      841 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
      901 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
      961 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1021 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1081 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1141 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1201 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1261 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1321 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1381 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1441 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1501 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1561 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1621 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1681 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1741 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     1801 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     1861 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     1921 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     1981 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2041 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2101 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2161 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2221 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2281 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2341 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2401 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2461 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2521 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2581 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2641 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2701 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     2761 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     2821 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     2881 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     2941 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3001 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3061 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3121 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3181 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3241 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3301 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3361 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3421 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3481 ttccggataa tgtgttcggc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3541 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3601 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3661 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3721 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     3781 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     3841 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     3901 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     3961 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4021 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4081 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4141 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4201 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4261 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4321 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4381 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4441 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4501 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4561 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4621 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4681 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4741 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     4801 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     4861 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     4921 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     4981 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5041 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5101 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5161 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5221 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5281 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5341 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5401 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5461 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5521 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5581 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5641 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5701 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     5761 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     5821 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     5881 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     5941 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6001 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6061 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6121 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6181 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6241 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6301 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6361 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6421 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6481 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6541 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6601 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6661 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6721 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     6781 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     6841 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     6901 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     6961 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7021 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7081 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7141 tgagcgcgcg atccagcacg gtcaggcgat tgccgccggg ataaaagcag cgaaagatct
     7201 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7261 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7321 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7381 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7441 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7501 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7561 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7621 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7681 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7741 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     7801 tcatattact ggtctgatga cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     7861 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     7921 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     7981 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8041 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8101 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8161 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8221 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8281 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8341 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8401 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8461 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8521 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8581 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8641 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8701 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     8761 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     8821 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     8881 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     8941 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9001 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9061 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9121 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9181 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9241 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9301 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9361 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9421 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9481 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9541 ctgcgtgaag atcacaccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9601 cctgccgatg atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt
     9661 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9721 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
     9781 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
     9841 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
     9901 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
     9961 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10021 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10081 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10141 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10201 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10261 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10321 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10381 gatgtccaaa tccaagggta acgttattga cccgctggat atggttgacg gtatttcgct
    10441 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10501 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10561 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10621 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10681 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10741 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    10801 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    10861 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    10921 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    10981 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11041 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11101 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11161 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11221 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11281 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11341 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11401 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11461 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11521 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11581 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11641 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11701 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    11761 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    11821 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    11881 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    11941 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12001 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12061 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag gtaataaagc
    12121 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12181 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12241 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12301 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12361 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12421 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12481 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12541 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12601 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12661 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12721 cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac
    12781 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    12841 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    12901 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    12961 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13021 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13081 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13141 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13201 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13261 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13321 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13381 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13441 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13501 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13561 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13621 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13681 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13741 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    13801 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    13861 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    13921 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    13981 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14041 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14101 aattgagttt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14161 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14221 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14281 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14341 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14401 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14461 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14521 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14581 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14641 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14701 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    14761 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    14821 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    14881 aacgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    14941 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15001 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15061 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15121 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DAJTRH010000089        14532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTRH010000089 DAJTRH010000000
VERSION     DAJTRH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(113..379)
                     /locus_tag="OO339_003063"
     CDS             complement(113..379)
                     /locus_tag="OO339_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5927607.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="OO339_003064"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="OO339_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5927608.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="OO339_003065"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="OO339_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="OO339_003066"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="OO339_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5927609.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="OO339_003067"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="OO339_003067"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5927610.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO339_003068"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO339_003068"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5927611.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO339_003069"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO339_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5927612.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="OO339_003070"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="OO339_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO339_003071"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO339_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14095)
                     /locus_tag="OO339_003072"
     CDS             complement(12641..14095)
                     /locus_tag="OO339_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5927613.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14532)
                     /locus_tag="OO339_003073"
     CDS             complement(14092..>14532)
                     /locus_tag="OO339_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5927614.1"
                     /translation="QDEQGRFSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGTSLVS
                     LVPVSELWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIP
                     IENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3872 a         3670 c         3431 g         3559 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgcac gaaccacggc agcctgacgg ctgcaaattg aaaaccgccc
    14521 ctgttcgtcc tg
//
LOCUS       DAJTRH010000090        14482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTRH010000090 DAJTRH010000000
VERSION     DAJTRH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(52..507)
                     /gene="csgB"
                     /locus_tag="OO339_003074"
     CDS             complement(52..507)
                     /gene="csgB"
                     /locus_tag="OO339_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5927615.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1261..1911
                     /gene="csgD"
                     /locus_tag="OO339_003075"
     CDS             1261..1911
                     /gene="csgD"
                     /locus_tag="OO339_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5927616.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1916..2305
                     /gene="csgE"
                     /locus_tag="OO339_003076"
     CDS             1916..2305
                     /gene="csgE"
                     /locus_tag="OO339_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5927617.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2330..2746
                     /gene="csgF"
                     /locus_tag="OO339_003077"
     CDS             2330..2746
                     /gene="csgF"
                     /locus_tag="OO339_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5927618.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2773..3479
                     /locus_tag="OO339_003078"
                     /pseudo
     CDS             2773..3479
                     /locus_tag="OO339_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3612..4103)
                     /locus_tag="OO339_003079"
     CDS             complement(3612..4103)
                     /locus_tag="OO339_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5927619.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4205..4759)
                     /gene="ycdY"
                     /locus_tag="OO339_003080"
     CDS             complement(4205..4759)
                     /gene="ycdY"
                     /locus_tag="OO339_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5927620.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4783..5520)
                     /gene="ycdX"
                     /locus_tag="OO339_003081"
     CDS             complement(4783..5520)
                     /gene="ycdX"
                     /locus_tag="OO339_003081"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5927621.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5575..6513)
                     /gene="ghrA"
                     /locus_tag="OO339_003082"
                     /pseudo
     CDS             complement(5575..6513)
                     /gene="ghrA"
                     /locus_tag="OO339_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6748..6835
                     /locus_tag="OO339_003083"
     tRNA            6748..6835
                     /locus_tag="OO339_003083"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6782..6784,aa:Ser,seq:gga)
     gene            complement(6985..7266)
                     /locus_tag="OO339_003084"
                     /pseudo
     CDS             complement(6985..7266)
                     /locus_tag="OO339_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7548..8552)
                     /locus_tag="OO339_003085"
     CDS             complement(7548..8552)
                     /locus_tag="OO339_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5927622.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8624..9184)
                     /locus_tag="OO339_003086"
                     /pseudo
     CDS             complement(8624..9184)
                     /locus_tag="OO339_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9269..9466)
                     /locus_tag="OO339_003087"
     CDS             complement(9269..9466)
                     /locus_tag="OO339_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927623.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9478..9969)
                     /locus_tag="OO339_003088"
     CDS             complement(9478..9969)
                     /locus_tag="OO339_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927624.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(9966..10340)
                     /locus_tag="OO339_003089"
     CDS             complement(9966..10340)
                     /locus_tag="OO339_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5927625.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10387..10761)
                     /locus_tag="OO339_003090"
     CDS             complement(10387..10761)
                     /locus_tag="OO339_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5927626.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(10924..11145)
                     /locus_tag="OO339_003091"
     CDS             complement(10924..11145)
                     /locus_tag="OO339_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5927627.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11208..11684)
                     /locus_tag="OO339_003092"
     CDS             complement(11208..11684)
                     /locus_tag="OO339_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5927628.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11700..12173)
                     /locus_tag="OO339_003093"
     CDS             complement(11700..12173)
                     /locus_tag="OO339_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5927629.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12515..13336)
                     /locus_tag="OO339_003094"
     CDS             complement(12515..13336)
                     /locus_tag="OO339_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5927630.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13436..13609)
                     /locus_tag="OO339_003095"
     CDS             complement(13436..13609)
                     /locus_tag="OO339_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5927631.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13729..14178)
                     /locus_tag="OO339_003096"
     CDS             complement(13729..14178)
                     /locus_tag="OO339_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927632.1"
                     /translation="MSWSHYLSPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGD
                     ATYRAAIDKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDY
                     IVTLYNPKTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            complement(14175..>14482)
                     /locus_tag="OO339_003097"
     CDS             complement(14175..>14482)
                     /locus_tag="OO339_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HCR5927633.1"
                     /translation="RKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRL
                     KGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         3695 a         3555 c         3465 g         3767 t
ORIGIN      
        1 aaaagtttca tgtaaaaccc ccatcggatt gatttaaaag tcgaatggta attaacgttg
       61 tgtcacgcga attgccattt gcgactgtct ctgcactaca actgccgttt tttgagtacc
      121 atactgtgta atatttgctt tattaccaga acctttctgg ataatcatcg cagtattacc
      181 ataagcacct tgcgaaatac tggcatcgtt ggcactgccc gcctgatcaa tatatgcaag
      241 gttataatct cctgtctggt caatctttgc ccgattgcta ctaccttctt gcgcaacaac
      301 cgccaaaagt tttgagcctc cctgccgtaa ctgagcacta ttattagtcc cagcctgacc
      361 aattatggct gcctgattaa atgaagactt actcaattca tttaccgcga agttatattc
      421 tgaattagct aaatcataac ctgctgcggc tgcaatccca ggcgcaccca gtattgttaa
      481 catcataaat aacaatttgt ttttcatgtt gtcaccctgg acctggtcgt acatttaaga
      541 aattaaatca tttcaacttg gttgttaacg caacctgtat tttgttaacg ctgcgttacg
      601 atggaaagta tgtctgcgga aatattttta ataactcacc cgcgcgttgt atttttcttt
      661 ttttgttcat accaaagtat taaaacccgc gtaaatacgc tgttatctac gcaaaaaata
      721 ttttgttttc tcttaaatct ccgttttccg ctatcaaaaa tcaccagaca gtcattcttc
      781 ttgcccgtcg ctgattgctg cgatatgtct tgcgcacaag ccgtgacaaa cctcgtcttt
      841 ttgcagtaaa aaaattgtcc acggaggtgt ggagaaaaaa caaaaacgtt ttacatgacg
      901 aaaggactac accgaaatat tttttatatg tattattagt aagttagcac catttgtatg
      961 attttttaaa attgtgcaat aaaaaccaaa tgtacaactt ttctatcatt tctaaactta
     1021 ataaaacctt aatgttaaca ttttaatata atgagttaca ttttgttaca tgtttaacac
     1081 ttgatttaag atttgtaatg gctagattga aatcagatgt aatccattag ttttatattt
     1141 tacccattta gggctgattt attactacac acagcagtgc aacatctgtc agtacttctg
     1201 gtgcttctat tttagaggca gctgtcaggt gtgcgatcaa taaaaaagtg gggtttcatc
     1261 atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac taaaccttct
     1321 ttgcaggcga cagctctctt gcagcacctt aaacaatcgc tagcaattac aggaaaatta
     1381 cataatattc aacgttctct ggacgatatc tcttcaggct ctattattct tctggatatg
     1441 atggaagcgg ataaaaagct tatccattat tggcaggata ctttgagcag gaaaaacaac
     1501 aatatcaaaa tattgttgct aaatacgcct gaagattacc cgtaccgcga cattgaaaac
     1561 tggcctcata tcaacggcgt tttttatgcc atggaggatc aagaacgtgt tgtcaatggg
     1621 ttgcaaggcg tcctgcgtgg cgaatgctac tttacgcaaa agcttgccag ttacctgatt
     1681 acacattctg gtaactatcg ttataacagc acggaatcag ccctccttac tcatcgggaa
     1741 aaagagatcc tgaataagct gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg
     1801 ttcatcagcg aaaatacggt taaaacgcat ctttataatc ttttcaagaa gatagccgta
     1861 aaaaaccgga cacaagcggt ttcctgggca aacgataacc tcaggcgata aagccatgaa
     1921 acgttattta cgctggattg tggcggcaga atttctgttc gccgcaggga atcttcacgc
     1981 cgttgaggta gaagtcccgg gattgttaac tgaccatact gtttcatcta ttggccatga
     2041 tttttaccga gcctttagtg ataaatggga aagtgactat acgggtaact taacgattaa
     2101 tgaaaggccc agtgcacgat ggggaagctg gatcacaata acggtcaatc aggatgttat
     2161 tttccagact tttttattcc cgttgaaaag agactccgag aaaactgtcg tctttgcact
     2221 gattcaaact gaagaagcac taaatcgtcg ccagataaat caggcgttat taagtacgga
     2281 cgatttggcg catgatgaat tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa
     2341 acatgcagta gttctactca tgcttatttc gccattaagt tgggctggaa ccatgacttt
     2401 ccagttccgt aatccaaact ttggtggtaa cccaaataat ggcgcttttt tattaaatag
     2461 cgctcaggcc caaaactctt ataaagatcc gagctataac gatgactttg gtattgaaac
     2521 accctcagcg ttagataact ttactcaggc catccagtca caaattttag gtgggctact
     2581 gtcgaatatt aataccggta aaccgggccg catggtgacc aacgattata ttgttgatat
     2641 tgctaaccgc gatggtcaat tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc
     2701 gaccatccag gtttcgggtt tacaaaataa ctcaaccgat ttttaagccc cagcttcata
     2761 aggaaaataa tcatgcagcg cttatttctt ttggttgccg tcatgttact gagcggatgc
     2821 ttaaccgccc cgcctaaaga agccgccaga ccgacattaa tgcctcgtgc tcagagctac
     2881 aaagatttga cccatctgcc agcgccgacg ggtaagatct ttgtttcggt atacaacatt
     2941 caggacgaaa ccgggcaatt taaaccctac ccggcaagta acttctccac tgctgttccg
     3001 caaagcgcca gggcttacaa aacctgctta acgagcgcaa ggttattcgt gcggcacaag
     3061 aaaacggcac ggttgccatt aataaccgaa tcccgctgca atctttaacg gcggcaaata
     3121 ttatggttga aggttcgatt atcggttatg aaagcaacgt taaatcgggc ggtgttgggg
     3181 caagatattt tgggatcggt gcagacacgc aataccagct cgatcagatt gccgtgaacc
     3241 tgcgtgtcgt caatgtgagt accggtgaga tcctctcctc ggtgaacacc agtaagacga
     3301 tactttccta tgaagttcag gccggggttt tccgctttat tgactaccag cgcttgctgg
     3361 aaggggaagt gggttacacc tcaaacgaac ccgttatgct gtgtctgatg tcggctatcg
     3421 aaacaggggt cattttcctg attaatgatg gtatcgaccg tggtctgtgg gatttgtaaa
     3481 ataaagcaga acggcagaat gacattctgg tgaaataccg ccatatgtcg gttccaccgg
     3541 aatcctgaca gataaaaatg ccggaattaa taatccggca tttactgttt aagcgaaagc
     3601 gtgagtgccc ctcacgcttt atttttgatt tcctgctcac ggtgagccgt ctttgcacta
     3661 cgcgccgcca gccacaggcc gctgtttttc attgcgtaac caaataccag cccaagcgcc
     3721 agcgaaggta acaccagttt ccagtcaccc tgcccggcaa acgtcgcgca tgcgcctata
     3781 aacgtccccg gaacaaatga aagcagcagc tgtttggcct gaatacacat cagaaacgcg
     3841 acaataccgg ttatgacata accgagaatt tccagatgtg gtgccagtgc actaccgtaa
     3901 ataatgacca tcgcccacac cacgccacta agcagggttg cagcggagat cgccagccct
     3961 ttcagcccac cttgcgggca agcaaagtag gccgtacagc ccaggaagcc cgcccagctt
     4021 agtaagccca gggaaacagc cacccatccc cagataccgg agagaatgcc tgttgtgatt
     4081 gcaatagaga gaagtatgtt catgccgcgc atgatagcag aaaacgcggc catgaatgag
     4141 atcgagcaca cattttatgc aacaacaaat tatctgttgc agttatgagt gacgatgatc
     4201 acatttactc ttcagaatct tcttccagct cgtcccacat tgcactaatg gcatcacggg
     4261 ttagcggtgc catggtgcgc cagaaaggcg tggttgcatg ggcctccact ttgccgagga
     4321 acgcaccaca ccagggtaac agatactcac tgaacagtgt ttccagtgct tcgctctcat
     4381 cttccgttga ctgatcttcc agccaggaag ccgcaagcag caatgtgccg atgtgatcgg
     4441 ctggcgtatc cgccagtagc atccctcgtt cggaaagaaa agcgcgcact tccgcttccg
     4501 ttgcgtcctc aacccatgcg ctacgatatg gcggcacggc acattcatcg ccgataaaca
     4561 acgcattgta atcggcagag acttgcgtca tatcacagct tttctgtaaa cgcgtcagta
     4621 actcatcctg ctccagtggc cagttcgcag ccagtttccc ctcacgaatc agagtaaaca
     4681 gcggcaccag taaaggatct tgcggttggc ggtaatagag cgaacccagc acacgacaga
     4741 ggatagaaaa ctcgttcatt aatatatttc cattactagt aattaaaggt ctgcaaattc
     4801 cgcaatcggt gccataccgc gagattcaag gaagttcagt aagcggcgcg gagaaacatt
     4861 caaaatgcgc tctagcggaa aatctaccgc gtcgaggatt ttaagacact cttcaaattc
     4921 ccccatggta aacgcggtgt gagaatccga gcctaatgcc acccaacctc cagcatcacg
     4981 taccgctgca gctaccgcac gacagttgtc ttcactgccc ttacgtgagt gtaaaaatga
     5041 ggaattattg atttccagcg ccacctgatg tttcgcggct gcttcagcaa cggctttaac
     5101 atctatttca tatttcggat ttccgggatg gcttattata tgcacattgc cgctggcgat
     5161 agtggagatc atcgcttgtg tattggtcgc tttgtcatgt ggcgcaaaaa ccggctcatg
     5221 aaaaccggca ataatcagat ccagcgagtc aaacatttta ccgctgcagt caatttcacc
     5281 atcaacattt ttaatgttag cttcaatgcc gcgcaggatc cctaccccat caaccactcg
     5341 cggccagata cgcatgttaa tgaagtgcca gtgatgcggc gcatcttcca tatccgggcc
     5401 atgatcggtg atcgcaaaaa gtttaatgcc cttttgtttg gcctgggcaa tgtaatcact
     5461 caatgtgcta tatgcatgtg tgctggcaac ggtatgcata tgaaggtcga cgggatacat
     5521 aactctctcc tgtattcaat tcccgccaag gatagcagga atcctgatgc tttattagta
     5581 gccacgtgcg cggtcgactt gcccgcagac cctctcccct ttttcgagct gggcaatagt
     5641 gcgagaaatg tactccacag cttcagcggg acgggtaatc gcggcgacat gtggtgttat
     5701 cgtcacgcgt ggatgttgcc agagcggact ttcaggcggt aagggttcac gattaaaaac
     5761 atccagcatt gcgcctttaa ctttcccgct atccagcgcc gcgagcaggt catcttccac
     5821 aacatgaaca ccacgcgcca ggttgaggag atacgcgcca tcaggtaatt tttcgagtaa
     5881 ttgttgatta ataatgccga cggtttcagg ggtattcggt aacaaattaa tcaatacccg
     5941 acattggctc agaaatgcag acagttcttc ccgtccggca aagctttgca cgccaggcca
     6001 cgatttacgg gttcgactcc agcaacgcag cggaaagcgc taggtttgca gactctgagc
     6061 aactttacag cccagtacgc ctgcgcccaa aatgccgatg gtaaaatctt cccgatgata
     6121 ttcaggcagc ggttgccaat gcgaactatt ttgctggatg cgataatcgt caaaacgtcg
     6181 aaaccaatgc agcacctgac tgacagcata ttcctgcatt tgctcgccca taccggtatc
     6241 ttccaggcga aaaagtggaa cagaagggtt cagcatttca gggtgtgcct gtagcttgct
     6301 caaaatagaa tcaacaccgg ccccgagtgc gaacaccgct ttaagatcgc gccctgccag
     6361 catttcaaca ggaggatgcc agactaaaac ataatcagca gagtcattat ctccgctttt
     6421 ccatgccctg actcttgcct gaggaatagc tttgcgcagt gcctcaatcc accattgggt
     6481 atcgaacgtt gggtgataaa agatgatatc catactgact cccgaaaagc gttgtgcgaa
     6541 atttattcgc acttatcgtt atgatctaca aaggccacca gcataacaaa tccgtggtcg
     6601 gtggcaaaaa aagcagattt cgcttattaa aaccacacat tgattgaatg tttgaataaa
     6661 cgcgcgattt tttcaaaaag tttgttgacc tcaggtcatg atttccctaa attagcgccc
     6721 gttccagcaa gacaggaacg acaatttggt gaggtgtccg agtggctgaa ggagcacgcc
     6781 tggaaagtgt gtatacggca acgtatcggg ggttcgaatc cccccctcac cgccagatta
     6841 taagtgcatg ttattgttta acatgcgctt ttttattttt ccttccagac agtatgccat
     6901 cgggtatgcc atctgaaacc ggtttatatg ccccgcgatc atatcttatc ggacaatttc
     6961 cgatctttga gcatactggc aaagctatac tgccagcata tcaggatagt gttttgcgat
     7021 aatatcattc agtctgtcaa ccagttctgg ttttctgaac gtgatatgcg cggagccctt
     7081 cttaaagtat ctgatgctga acatctcatc ttcatagctc tctttaccct gaacagactg
     7141 aatgtgatca tccagacgaa gggtgatgtt tccccggttg tcggggattg gtttaccgct
     7201 gaacaggtgc aacattcttt ccagatcggc gagtcggtca gcctgttgtc cggtgataag
     7261 atgaaatcta tggtcacccc cgtttttgca acactgattt ttgattaatg gttggcttgc
     7321 ttaaatctat ccggcgtctc tgtgggcaag gatgcccgcg ccccgatgag ttccgcacct
     7381 gataatccta aaaatcccct cggcttcagt gagccatttc atctgttagg tttttaatca
     7441 ggtcgatttg ccgttcatgt catcaataat cagtcttcgc aaaaccagaa aggtaacttg
     7501 tatcttattg tttaaaataa actaattgag ttatatcagc atctatatca ggcctgatat
     7561 tcttttttct gagtagtgat tgcccaggca attcgagcca gtttatttgc cagagcgcag
     7621 gtaaccacat tcgaatgctt cttgttgagt tgctcccgaa cccactcagc aagttttcca
     7681 tgcttgcgtt caagtcgcat tatgaatgag cgggcacact gaaccagtaa ccggcgtaaa
     7741 tttttatcac ctcgcttact gatacccaac agagtcgatt ttcctccagt gctatactgc
     7801 cggggaacaa gtcctgtgga agctgcaaaa tccctgctgc atgagaactg tttcccgtca
     7861 ccaagttgcg acgatagtaa gctggcagta atcggaccaa caccaggaat tgtcataatg
     7921 cgctgagcgg tatcatcagc gttgattgac tgacttaatt ctgattccag ctctgctatt
     7981 tgctcaacaa ggtaaaagta gtgggtatgc aacctcacca aaaggcggct cagatattcg
     8041 gggatttcgt gttcagccaa cacgagagac aaccgtttta ttacagcatc acctatcggc
     8101 aggctgattc caaattccag caagaaaccg tggatctggt tggttgtttt caccttatct
     8161 ctgatgagcg attctctgac ccggtgtaac gccctcatgg cctgctgggt ctcagttctt
     8221 ggctggacaa aacgcattga tggtcgtgaa gccgcttcgc atatggcttc tgcatcaaca
     8281 aaatcattct tattactttt gacaaacggg cgaacgaact gtggagaaat cagcttagtt
     8341 tcatgcccaa aatcagagat acagcgagcc ataaagtgtg cacccgcaca ggcttccatt
     8401 acaacaactg agggcggaca tgtcgccaga aactgcatca gtttagtacg ggtaaatttt
     8461 ttacgcagaa gagccttacc agacttgtct tgacaatgaa tgtggaagga gtgctttccg
     8521 agatcgatac caataagtgt gatgttctgc atgatggtct ctccgttaaa gggatttacc
     8581 ttaacagcgt actgctgtta aggtgggggt gaccatatca ttatccccac cggtcccatc
     8641 tcaccagatt gttgacgata attttactgc caaatttaca ggggcaattg gttttgtaat
     8701 tccagttcag ccctctgaag acgttgatca cccctcgctc aaacacctca tccttattct
     8761 ggtgcagttg ttcaaatgtg ctcagaatgt tcgcttcgct gatttccgga aaattatcgt
     8821 actccagtga ccggtaccat gtatcacggg cctgcgtatc cattaatgac agcatcccgg
     8881 aacgttgcat caggtcacgc cagatttcgc gatcaagatt ccgggtaatg gctttcattg
     8941 ctgtttcggg tttctccatc agccagcagc cgcagcgaaa atcctgtttc atggcccagt
     9001 ccggggcagt tttacctccg atgctttccg tcagcaccga gatttcagcc agttgtcgga
     9061 taagtgtgtc gatttgcaac agtgtactct ttcgtacagc cagaatgtgg cgaatgctgg
     9121 ttgagcagat aacgtccgta tgcccgttga ccacttccgg acgggagatt gtgatatctg
     9181 acataatcga tattcctttt tgcagataaa tgaaaacgcc agccctgtgc aggctggcgt
     9241 cagaggaatg aagtctggtg agtgagcttc accggttctg gtgcagaagc tgcagatgac
     9301 gcagggtacg gacatcctcc cgggcctgtt cgatacaggg caacagtgct gctgaatccg
     9361 ttttatcctc atcgttgtca aagataattc ccgatccgca gtcgatattg tcctgcagcc
     9421 acgcaatcag aatatccagc gctgtttccg tggttaatga tttcatgttg ttcctggtta
     9481 ccagtcgaaa gtgggtaaac ccggcaggca ttcagcgctg gcatccagat gaatgagact
     9541 gacgccataa cgccggatga gtgtgacgac cagacgacgg aacgtttttg acagccccag
     9601 gcgtttaagc cgcagaaccg ggtatgacca cgcatcggta cgtaacagat atccgctacc
     9661 tgtaaaatga atccattctg attcaccgaa gtcactggtc tggtgtgaca gcgagtacaa
     9721 ccaggcgttg tccgtttccg tgatatgtgc ggtactgcag cgtatgccgg taagggtagc
     9781 aaatggtggc gtgcattctg ctgaggtctg gtcagattca tcaacaacac gaagggagta
     9841 gccgttatcg cagaccatgt taatcagttc agcgagctca ataccatcga cgtcaacgac
     9901 aatgcgccct atgttcagtg cctgtacgtt aacgctgtcg gcttccagtg tcagggccag
     9961 tttcatcatt tcgcctccgg atacttaccc agggtaatgt tatttaccgt tctgtaattg
    10021 tcgcgggtca tcaggccggt cgcccggcga gcccggagga tatcaatact gtttattaac
    10081 tgagagcggg gacaggtgct gaatcccggc tggtcggtac gcaccagcgc gtatttttcc
    10141 acgagaaaat taactgcatc acacagtgaa atgcctgcct caatatgctg ctcaatcaca
    10201 cgttcatcga caaacggtgt gtcattcagt gtgaggccat aatgctggtc cagcagtcgg
    10261 atgagcagtg tctgccagat ggtgacggga gacgggcagc gagatacctc ccgtacatgt
    10321 gtgtcggata atgttttcat cttaagttct ctgtaactgg tagtgctaaa ggtgagagct
    10381 tcggggttat gaggtggttg cgggtgctgg tgtcggataa acagccagat aaacgtaacc
    10441 acaggagccg agggtgtcgg cttcgcaggt cagccctttt gcatacagcg tgacggtatg
    10501 ctgatatcgg ggattcagtt caccgctggt gagcatgagt tccagttgtt tcatcagcag
    10561 cggaaaggcc tggtccagat ggtatgcatc tgcgtcgctg aagcggcctc tgataccggc
    10621 gcggtcggca aggtaataca ggcggttacc ctcttgcacc agacgagcgc caaaacaggg
    10681 cctcactgtg cagggaagcc cccaccaggg gcggtcgtga ttgtcatcgg gatgagttat
    10741 cccggagagt ttgtctgaca cgataaaatc ctctctgaaa agtggttaag aatggtttaa
    10801 cgatgacagg tcagccggcg cgctgaccgg tttcggtgaa gatgtatgca ttgatgagaa
    10861 tggcctcatc cacagcggcg tctgaggtca tctggtcgta ctcgtttccc gggtgacgga
    10921 acatcagttc agggtgacac tcatcagccg gacatacggg ccaaaactgt ccttacggcg
    10981 ttctgcaaac acggcaagaa cgccgggaat atcctgcact tcacgaccag tatacgcttc
    11041 agcactgccg tgccagcgat acttaccggt acagaacgga aaaagacggg atgcaggatg
    11101 ctgttggtga atacgcatgg cttcaccacg ggtgatgatt ctcatgatgt gatacctctg
    11161 aagacagaag ataaaagtga aaacaggtgt gatgtggttg tgacgggtta aagcagacca
    11221 tgttcggcaa aggagaaaac ctggttgcca ccgactatca gatggtccgg cacccggata
    11281 tccaccaggc ccagtgcctg taccagacgt tcggtgataa gccggtctgc cttactgggt
    11341 gtgacttcac cggacgggtg attgtgcgcc agcaccacgg cagcggcatt gtggtacagg
    11401 gcgcgtttaa tcacttcccg gggatgaact tccgtacggt tgatggtgcc ggtgaagagg
    11461 gtttcaccgg caatcagctg attctggttg ttcagataca gcacccggaa ctcttcccgc
    11521 tccagtcccg ccatgttcag aatcagccat tcccgtgccg cacgggtgga ggtgaaggcc
    11581 acgccgggtt catgaagatg gcggtccagg gtttgcaggg cccgcagaat gagactgcgc
    11641 tcgccgggcg tcatgtttcc gggcagaaag gaaagctgct gcattgtccc tgcctccatt
    11701 cagtcgatga tgcgcataat ggcgctgcat tccggatgct gcagggcgta atcccgcagc
    11761 cggtaataat ggaccgtcat ggcataacac tccgtacgac aggcatgatg actgtacgtc
    11821 atcagacagg cagcaatgcc ggcggcttcc gggctcattt cagcgcggtt accgttcatg
    11881 gcattgaaca gtacccatgt ttcgtcatca tcgtcatccg gttcgggtgc cataaatgcc
    11941 ccgccgttgt tcagggtgta cagattccag ataccaccgc agtagtcttc acacagacgg
    12001 tccatccagc cgaagacacg gggttccagg gtgacccact gtggaatgag gccaaaatgc
    12061 tgcggccaga agctgatgcg ctgttcatca gggacgggag tggcaaccag ttgttgcagg
    12121 ccagtctccg gtgcacaggc cgaagcggtg gtgttctgag acaatgtttt catgtatgaa
    12181 atccttgtaa tgtgaataaa tgagagggag taaaggtcgg gagctggcaa catcacgttc
    12241 atcactgacc gtgatgaccc ggtaatatca gggatgaatg atattcggct gaaattccgg
    12301 acagccgtac gcgtaaccgt tccggtcaca gacggatggc cagatgccat tgcctgtata
    12361 ctcagtctgc acagactgat ttctccggga cggtaaagca gacgggtaca ccaggcgcac
    12421 aggtcatcat cagaaatgga acgacacccc ggcagcgcct ccggggcgtc gggtgattct
    12481 ggtgcctgaa tcacgccttc agggacatgc tgcctcagcg caggctctcc agcagcgttt
    12541 ctgccattac ccacaatgcg cggttgagct taatatcggt gtcgatgttg tgaatggcac
    12601 gggtgtggat acgttttcct tttgcactgc ggccggaaat cccgcctttc agcatattct
    12661 cctggatggt ctgatatgcg ctccacaggt ctttaccgta atcctccctg cgtcgtggtg
    12721 tcagaatgtc agcggtggtg actggctgat gttcgtcacc ataacggtaa gtcagtgccg
    12781 cctgtgccag cgcctggcgt gccggtggcg gcaggaccag cgactgcatg gcatcacgct
    12841 tctcctctat ccggtcaaac acgcccacca cctcgtaagc cccttcaata actttctcca
    12901 ccacatttcc ccggtgtgga acacgcaatt ctcccagaga ctgaccgcag accagtccat
    12961 tggtacaaat ggcacgaaaa tatccgggta acatctggta gctggatgaa ccgtcatgag
    13021 agttgagcag aataatttca gggacatgct gaccggttat ctgtccggcc cgccgcagac
    13081 gcagcatgtg ttttgtgtat tcccggcgac ccgggtcacg cacacgtgtc tggcaggcga
    13141 agaatggctg aaagccttcc cgctgcaggc tttccagtac ggtgatggtg ggagtgtacg
    13201 catagcgttt actgcgggaa gtatgccggt cttcaccgaa aatactggga acatagtgca
    13261 tcagttcttc gtgtgtcagc ggacggtcac ggcgtatctg gtttgcagca taaccaaaac
    13321 gactggctag tcgcataatt tgctccttat cggtggttaa gatttactgg tgtaataaat
    13381 gaaaaagcca cgtctcccgg agaagacgcg gcctgatgga cgaaattaat gaggtttatt
    13441 gtctgagaag accgtcactg gcgaaccttc cggcgtcagt ctctgcgttg cggataaccc
    13501 gacggaaatg agttccctgg tcatcgtcga taaacacatt gcggtaagct gttgtgatga
    13561 caacagcctg ctcgcgtgag aaagggactt cgggcaataa acgtgacata caaccagacc
    13621 tgtatttggt tccctgaaaa tataagtccc tgtcatcatg gcagagccgt atttactttt
    13681 gatatcaata aattaataac gcgaagatga accgcataac ttacgccttt aatttgtttt
    13741 gtttacgtag tccctgacca ggtacgcgaa ttcgtgaacg ccagtttcac caaaccaaat
    13801 caggtaacca gggtcgatgt tgtaatagta accaacgact ttatgcgttt ttgggttata
    13861 gagtgttacg atatagtccc agccgcaggc gtgggcttta caaccgccac cttctaccca
    13921 ggtcgttccg ttaatgttta caggatacgc accaccatca ggaccatcca tatagccgtc
    13981 ttccccaaac attccgttta acccaacagg gtttaatgct ttgtcgatag cagctctgta
    14041 ggtagcgtca ccttcgtata actcgtacgt tgttggacat gcaccaggct ggtcagcccg
    14101 ggcttttgag tcgcatttaa gtgtctgatt tacagtggcg gcgtttgtgt caggacttag
    14161 gtaatgactc caacttattg atagtgtttt atgttcagat aatgcccgat gactttgtca
    14221 tgcagctcca ccgattttga gaacgacagc gacttccgtc ccagccgtgc caggtgctgc
    14281 ctcagattca ggttatgccg ctcaattcgc tgagtgtaac gcttgctgat tacgtgcagc
    14341 tttcccttca ggcgggattc atacagcggc cagccatccg tcatccatat caccacgtca
    14401 aagggtgaca gcaggctcat aagacgcccc agcgtcgcca tagtgcgttc accgaatacg
    14461 tgcgcaacaa ccgtcttccg ga
//
LOCUS       DAJTRH010000091        14177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTRH010000091 DAJTRH010000000
VERSION     DAJTRH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..924)
                     /gene="fabY"
                     /locus_tag="OO339_003098"
                     /gene_synonym="yiiD"
     CDS             complement(<1..924)
                     /gene="fabY"
                     /locus_tag="OO339_003098"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5927634.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEG"
     gene            complement(969..1406)
                     /gene="dtd"
                     /locus_tag="OO339_003099"
     CDS             complement(969..1406)
                     /gene="dtd"
                     /locus_tag="OO339_003099"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5927635.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1403..2275)
                     /locus_tag="OO339_003100"
     CDS             complement(1403..2275)
                     /locus_tag="OO339_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5927636.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2269..2868)
                     /gene="yihX"
                     /locus_tag="OO339_003101"
     CDS             complement(2269..2868)
                     /gene="yihX"
                     /locus_tag="OO339_003101"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5927637.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(2967..3752)
                     /locus_tag="OO339_003102"
     CDS             complement(2967..3752)
                     /locus_tag="OO339_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5927638.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3786..4682)
                     /gene="yihV"
                     /locus_tag="OO339_003103"
     CDS             complement(3786..4682)
                     /gene="yihV"
                     /locus_tag="OO339_003103"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5927639.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            4850..5746
                     /gene="yihU"
                     /locus_tag="OO339_003104"
     CDS             4850..5746
                     /gene="yihU"
                     /locus_tag="OO339_003104"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5927640.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5770..6648
                     /gene="yihT"
                     /locus_tag="OO339_003105"
     CDS             5770..6648
                     /gene="yihT"
                     /locus_tag="OO339_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5927641.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6665..7906
                     /gene="yihS"
                     /locus_tag="OO339_003106"
     CDS             6665..7906
                     /gene="yihS"
                     /locus_tag="OO339_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5927642.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8044..8946
                     /locus_tag="OO339_003107"
     CDS             8044..8946
                     /locus_tag="OO339_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5927643.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9145..11181
                     /gene="yihQ"
                     /locus_tag="OO339_003108"
     CDS             9145..11181
                     /gene="yihQ"
                     /locus_tag="OO339_003108"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5927644.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11227..12601
                     /locus_tag="OO339_003109"
                     /pseudo
     CDS             11227..12601
                     /locus_tag="OO339_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12644..14047
                     /locus_tag="OO339_003110"
     CDS             12644..14047
                     /locus_tag="OO339_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5927645.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
BASE COUNT         3408 a         3708 c         3582 g         3479 t
ORIGIN      
        1 gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc cttcaaacac
       61 tgcacccggc gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg cttttcgtcc
      121 gcgcgccaga cggtcgagat cgccgcttaa ggcaccaagg tcggctaccg catggggttt
      181 accgctaatc ggtttgctgt agcggatatg cgcatccgcc agaataatcg ttccgccgag
      241 gtggcgttca cgcagcatca gccagataag cccccaaccg gtgagcgtcg ccagcgagaa
      301 taaactcccg gcaaacagcg tatggtgcgg attctgattg ccggtttccg gcatggtggt
      361 gataaatttt tgcccggtat attgttgaat acgcacgccc attttttcac taagcgggat
      421 atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca aaatatcatc
      481 cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg ttggcgtggt
      541 gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt cttcacgggc
      601 gctacaggtc acgcgcttaa cgccttcctg acgcgccacc gactccaggg tcatcgccat
      661 cagcgttcct aaccctttgt cctgcacgtc cgggtgaacg accataaagc gaatggacgc
      721 ttcattgtcg gcattaatat acaatcggcc taccgccacc agattaccct gctcgtcgac
      781 gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt ttggttgatg
      841 caggggctta cgcaacattt cccagcgaaa gtgatagtaa cgctctaact cttcttctgt
      901 ttgtggaacc tgaaggtgat acatagcgat actctctctt gtttcccgtg acaaccctgg
      961 aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag cgatacctgc
     1021 atatcggcag cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca gcgttcgacg
     1081 aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa acttgggcgc
     1141 atcccccgtt cggtatctgc ggcgagggta aactgggaaa ccaccagtac actgccgccc
     1201 gcctgttgca cgttgagatt catcttgcct tcggcatcgc taaagatgcg gtagccgagc
     1261 acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac acccaataac
     1321 accaaaagtc ccgcgccaat ttcgcccgtc acttctccct ccacggtgac gctggcacgg
     1381 gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca gctgcttgtt
     1441 ttagtttgcg gtattccccg agagtgacag taatttccgc gccaagcaag acgatacacc
     1501 atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg taaatgagct
     1561 gatatgacgg gaacatggtg atataaagcg cgaaaccttt ctttcctgct tcgaacagga
     1621 gtgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg gtaggaacaa
     1681 tgctgtacag caaccagaag gagatccacg acaacagcaa cggaaaaata cgcagcacgt
     1741 tgtcgatgac agtattgaga tcgctcgccc agcgcaggga gagcaaatag gaactgatcg
     1801 ccagactggc ccctgccagc agcggcccca gcgttaaaat catccagtac acggcgaacg
     1861 agtaaatttt tggtcgcgct cgtttactgc gccagatggt attcaacgcg ctatcgatgg
     1921 agtacatcaa caataacgcg gtgacgatca gcccgcacgc cccaacggcg gtcatcttgt
     1981 tggaattagc aacaaattgt tcgatatacc gctgaataac atcgccagta gcaggcagaa
     2041 agttggcaaa aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg ggaaaagcgg
     2101 cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac acataggcaa
     2161 ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt agttttagcc
     2221 aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt agcataacac
     2281 cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg taatgcctag
     2341 ctgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat cgctgggtga
     2401 aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc gcatccccag
     2461 atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt cttccggcca
     2521 gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc cctgctcacg
     2581 cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg cctgccagcc
     2641 gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg cctctgcgaa
     2701 cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca tatgaaaact
     2761 cttcttaagc gatgccagcg gaatacgcgt taaatcgctc caggctccca gcacacggtt
     2821 aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt ttgcctcctt
     2881 tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt ggcaaggtat
     2941 gaagccaggt taacggtcac aaaaatctac gcgtcttcct ggggaagaat gatttcaatt
     3001 tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt aaagagcgca
     3061 gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt agtgtgatcg
     3121 gcgaccagca gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt agcttcgttt
     3181 acatcaaact ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt taccagataa
     3241 tcagcacgaa aatcagccac aaaggacgcc gctgaagggc caatgatccc gctattatga
     3301 gagcgcaacg taccgccggg caccatcact tcaaagcgcg ggttgtggta aagaatatgc
     3361 gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa cgcccgggca
     3421 acatgctcaa cagtcgtacc aatggtgata aatattgttg aaccatcagg aatatagtct
     3481 gccaccgctt cggcaatcgc tttttttttc tcggtttgcg aaacctcacg ctgctcgaac
     3541 gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt aatcaggcct
     3601 tgctcgctta atttacgaat atcccggcgg accgtctgcg tggagacatc cagcagattt
     3661 gccagctcat caatattcat atacccacgc tcggcgatca gcatgaggag ttggtcgtgc
     3721 cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac catcaccctg
     3781 gcattttata caaaaagtga caaaaaagat cgggtttgat cacagtcagg gatcccggct
     3841 cgtccaccgg gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg gactgactcc
     3901 gctaaatccc cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac atctcccgca
     3961 cctgtggtat ctaccacatc aactttgaag gccggttgat gctgacgccc accattttcc
     4021 agccagtcgc agcctgcgct accctgggtc acatagacat gaccatttgt gagcgtttgt
     4081 gcctgtttta gcgcactggc catctctttc acgcccgtta agcgcgccag acccggttct
     4141 gaaaaggccg cgtgatcgct taatgccacc agctcactga tatcctgcgg cgtaatgtcc
     4201 ccgtccagaa cggtcatcac acccgcctga cgggccaggg tgaaggcttt tttagcgccg
     4261 tcgtgccagc gtacatctgc cagcacaaca tcccactgag agaaatcaat ttcctccaac
     4321 cactctgcgt caggcagcag atccgggctg gggtagttaa tgattatccg ctcgcctttg
     4381 gtatccacca tgatggcgga ttgcgaagat ttcgcctggt tataccgttt ggtgtaacgg
     4441 gtgttaacgc cccaggattc cagctctgcc agcaggctgt tgcccgcgtc gtcatcacct
     4501 acgcgaccaa taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc tgccgtcgcc
     4561 gctggcccgc caccaacttc cgtataattt ctcgccacgt atttaccgct ctccgtcggt
     4621 aacccttcca cgtaatagat gcgatccatc acggttatac ctacacaagc aacacgaatc
     4681 atggtcattc cttaaacatt ttgggatgcg ttcattttaa tttcaccaat tgtttaaaaa
     4741 gtgacggctg tcaatttttt gacgataaat tacaaattcg atcaaaaaca gacaagcaaa
     4801 gcatgtcata aatgacaaaa agtgacatca ctgtattcag gagagggtta tggcagcaat
     4861 cgcgtttatc ggtttaggac aaatgggttc gccaatggcg agcaatttat tgcagcaagg
     4921 gcatcaactt cgcgtctttg atgtgaacgc cgaagctgtg cggcatctgg tagacaaagg
     4981 tgcgactccc gccgccaacc cggcgcaggc cgctaaagat gccgaattta tcattaccat
     5041 gttgccgaat ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg tttgcgaagg
     5101 cttatctacc gatgcgctgg tcattgatat gtccaccatc catccgctgc aaaccgataa
     5161 attgattgcc gatatgcaag ccaaaggctt cagcatgatg gatgttccgg taggccgtac
     5221 ttctgcaaat gccattaccg gtactctgtt actgctggct ggcggcaccg ctgaacaagt
     5281 tgaacgtgcc acaccgatcc tgatggcgat gggcagtgag ttgatcaacg ctggcggtcc
     5341 gggcatgggg atccgcgtta agctcatcaa caactacatg agcatcgcgc tcaatgcgct
     5401 ttcggcagaa gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg ttgccgtcaa
     5461 agtgatgagc ggtaccgccg ccggtaaagg tcacttcacc acttcctggc caaacaaagt
     5521 cctcagcgga gatctttctc ccgccttcat gatcgatctt gcccataagg atcttggcat
     5581 cgcccttgat gtcgccaacc agctgcatgt gccaatgccg ctgggggccg cctcacggga
     5641 ggtttatagc caggcgcgcg cagcgggtcg cggtcgccag gactggtccg ccattctgga
     5701 acaggtccgt gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac tggataaagg
     5761 aataaatgaa tgaataagta caccatcaac gacattacgc gcgcatcggg cggttttgcc
     5821 atgctggcgg tcgatcagcg cgaagccatg cgcatgatgt ttgccgcggc tggggcaccc
     5881 gctccggtag ccgatagcgt tttaactgat ttcaaagtta acgctgcaaa ggccctctcg
     5941 ccttatgcct cggcgattct ggtagatcaa caattctgct accgccaggt ggttgagcaa
     6001 aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg atgagttcat tcctggcaac
     6061 ggtattccgg tcgatagcgt ggttattgac cgcaaaatca atccgctaca gatcaaacaa
     6121 gacggtggca aagccttaaa actgctggtg ctgtggcgta gtgatgaaga tgcgcagcaa
     6181 cgtctggata tggtgaaaga gttcaacgaa ctgtgccact cacacggtct ggtaagcatc
     6241 attgagccag tcgtccgccc accgcgtcgt ggcgataaat tcgatcgcga acaagcgatc
     6301 atcgatgccg ccaaagagct gggcgacagt ggcgctgacc tctacaaagt tgaaatgccg
     6361 ctttatggca aaggtccgca acaagagctt ctctgtgctt cacaacgtct gaatgaccat
     6421 atcaatatgc catgggtgat cctctcttcc agtgtcgacg aaaaactgtt cccgcgtgcc
     6481 gtacgcgtgg caatgacggc gggcgcatcg ggattcctgg caggccgtgc agtctgggca
     6541 tcggtcgtcg gtttaccaga caacgagctg atgctccgtg acgtttgcgc accgaaatta
     6601 caacaacttg gcgatatcgt cgacgaaatg atggctaaac gccgctaaga tgaggataaa
     6661 aggaatgaaa tggtttaaca ccctaagcca caaccgttgg ctggaacagg aaaccgaccg
     6721 catctttgat tttggtaaaa attccgtagt gccgactggt tttggctggt taggcaataa
     6781 agggcaaatc aaagaagaga tgggcaccca tctgtggatc acagctcgta tgttgcacgt
     6841 ttattccgtt gctgcggcga tgggtcgacc tggcgcttac gcgttggttg atcacggtat
     6901 taaagccatg aacggcgcac tgcgtgataa aaaatatggc ggctggtatg cctgcgtgaa
     6961 tgacgagggc gtggtggatg cctccaaaca gggctatcaa cacttctttg ctctgctggg
     7021 tgccgccagc gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg attacaccat
     7081 tgagattatc gagaaatatt tctggagcga agaagagcag atgtgcctgg agtcctggga
     7141 cgaagccttc agcaaaaccg aagagtaccg cggcggcaat gccaatatgc acgcggtgga
     7201 agctttcttg attgtttatg acgtcactca cgacaaaaaa tggctggatc gcgcgattcg
     7261 cgtggcttcc gtgattatcc acgacgtcgc cagaaataat cattatcgcg ttaacgaaca
     7321 cttcgatacc cagtggaatc cgctgccgga ttacaacaaa gataacccgg cgcatcgctt
     7381 ccgcgcgttc ggaggtacac caggccactg gatcgaatgg ggccgtttaa tgctgcacat
     7441 ccatgctgcc ctggaagccc gttgcgaaca accaccagca tggctgctag aagatgccaa
     7501 aggtctgttt aacgccaccg tgcgcgatgc ctgggcaccc gatggagcgg acgggattgt
     7561 ttataccgtt gactgggaag gaaaaccggt ggtccgcgaa cgtgtacgtt ggcctatcgt
     7621 cgaagcaatg ggtacggtct acgcgctcta caccgtcact ggcgatcgcc agtatgaaac
     7681 ctggtatcaa acatggtggg agtactgcat taagtacctg atggactatg aaaatggttc
     7741 ctggtggcag gagctggatg cggacaataa ggtcatcacc aaagtctggg acggcaaaca
     7801 ggatatttat cacctgctgc attgcctggt gatcccgcgt atcccgttag cgcccggcat
     7861 ggctccagcg gttgcagcgg gtctgctgga tattaatgcg aaataactga tctgaaaata
     7921 gggtgcgggt tttcccctca ccctaaccct ctccccaaag gggcgagggg accgtccggt
     7981 gcagtttttg ggtttgacat cagcctcacg ggaggctgta tgtcgttaat taaggtgcct
     8041 tgtatgcaaa tcaccaatat gcattgcagt ggacagaccg tttcacttgc cgcgggggat
     8101 tatcacgcga ccattgtgac cgtcggtgct ggtctggctg aattaacctt tcagggttgt
     8161 catttagtca tcccgcacaa acccgaagag atgccgctgg cgcatctggg taaagtgctt
     8221 attccgtggc cgaaccggat cgccaacggc tgttaccgtt atcaggggca ggaatatcag
     8281 ttacccatta atgaacatag ttcaaaagcc gctattcacg ggctgcttgc ctggcgagac
     8341 tggcaaatta gtgaattaac caccaccagc gttaccctga cagcttttct gccgcccagt
     8401 tacggctatc cgtttatgct ggcttcacaa gttgtttatt cgctcaacgc acacacgggt
     8461 ctttccgttg agatcgcaag ccagaatatc ggcactgtcg cagcgcctta tggcgtgggt
     8521 attcatcctt atttaacctg caacttaaca tctgttgatg aatatctgtt ccagcttccg
     8581 gcaaaccagg tttatgccgt cgatgaacac gtaaatccga caacgcttca ccacgttgat
     8641 gagctggatt taaatttcac gcaggcgaaa aaaatagccg cgacaaaaat cgaccacacc
     8701 tttaaaaccg ctaatgattt gtgggaaatg accattaccc atccacagca ggcgctttcg
     8761 gtaagccttt gttccgatca accctgggtg caggtttata gcggtgaaaa attacagcgt
     8821 cagggactgg cagttgaacc tatgagttgc ccgcctaacg cctttaattc aggtatcgac
     8881 ttattattac tggaaccagg aaaaccgcac cgcctgtttt ttaatattta cggacagaga
     8941 aaataacttg tattgattag cagtcaaaat cattgaagaa catttcactc gctaaaggca
     9001 acattatgta tcttcaattt attaagttaa aaacagctca gcttctgtaa agtttttata
     9061 tatcagtttt atgagaatca ttttacataa gcctgtaata acaggaaggc aacaatctcg
     9121 ttctcaactc tatggagatt agttatggat acgccacgtc cacagttatt agattttcaa
     9181 tttcatcaga ataacgacag ttttacccta cattttcaac aacgtcttat tttaacccat
     9241 aacaaagata atccttgttt atggattggc tcaggtatag cggatatcga tatgttccgc
     9301 ggtaatttca gcattaaaga taaactacag gagaaaattg cgcttaccga cgccatcgtc
     9361 agccagtcac cggatggttg gttaattcat ttcagccgtg gttctgacat tagcgccacg
     9421 ctgaatatct ctgccgacga tcagggccgt ttattgctgg aactacaaaa cgacaacctt
     9481 aaccacaacc gtatctggct gcgccttgcc gctcaaccag aggaccatat ctacggctgc
     9541 ggcgaacagt tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg gaccagtgaa
     9601 caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg caaagaaaat
     9661 gcgggcggcg actattactg gactttcttc ccacagccta cgtttgtcag cacgcagaag
     9721 tattactgcc atgttgataa cagttgctac atgaacttcg actttagcgc cccggaatac
     9781 catgaactgg cgctgtggga agacaaagct acgctacgtt ttgaatgtgc tgacacatac
     9841 atctccctgc tggaaaaatt aactgccctg ctgggacgcc agccagaact gcccgactgg
     9901 atttatgacg gggtaacgct cggcattcag ggcgggacgg aagtgtgcca gaagaaactg
     9961 gacaccatgc gtaacgcggg cgtgaaggtt aacggcatct gggcgcagga ctggtctggg
    10021 atccgtataa cctcttttgg caagcgcgtg atgtggaact ggaagtggaa cagcgaaaac
    10081 tacccgcaac tggattcacg cattaagcag tggtatcagg agggcgtgca gttcctggcc
    10141 tatatcaacc cgtatgttgc cagcgataaa gatctctgcg aagaagcggc acaacacggc
    10201 tatctagcaa aagatgcctc tggcggtgac tatctggtgg agtttggcga gttttacggc
    10261 ggcgttgtcg atctcactaa tccagaagcc tacgcctggt tcaaggaagt gatcaaaaag
    10321 aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta tctgcccacc
    10381 gacacgtacc tgcataacgg cgtcagtgcc gaaattatgc ataacgcctg gcctgcgctg
    10441 tgggcgaagt gtaactacga agcccttgaa gaaacgggca agctcggcga gatcctcttc
    10501 tttatgcgcg ccggttctac cggtagccag aaatactcca ccatgatgtg ggcgggcgac
    10561 cagaacgtgg actggagtct cgacgatggc ctggcgtccg tagtcccggc ggcgctgtcg
    10621 ctggcaatga ccggacatgg tctgcatcac agcgacattg gcggttacac caccctgttt
    10681 gagatgaagc gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc cttcacgccg
    10741 atgatgcgca cccacgaagg taaccgtcct ggcgacaact ggcagtttga cggcgatgcg
    10801 gaaactatcg cccattttgc ccgtatgact accgttttca ccaccctgaa accttatctg
    10861 aaagaggccg tcgcgctgaa tgcgaagtcc ggtctgccgg tcatgcgccc gctgttcctg
    10921 cattacgaag acgatgcaca tacctattcc ctgaaatatc agtacctgtt aggccgcgac
    10981 attctggtcg ctccggtaca cgaagaaggt cgcagcgact ggacgctcta tctgccggag
    11041 gataactggg tacatgcctg gacgggtgaa gcgttccggg gcggggaagt taccgttaat
    11101 gcgcccatcg gcaagccgcc ggtcttttat cgcgccgata gcgaatgggc ggcactgttc
    11161 gcgtcgttaa acagcatcta atcacatccg cccgtgggga aactcatggg caaagggaga
    11221 acatcgatga gtcacatcac aacggaagat ccagcaactt tacgcctgcc ctttaaagag
    11281 aaactctctt acggtattgg cgacctggcc tctaacatcc tgctggatat cggtacgctt
    11341 tatcttttga agttttatac cgacgttctg gggctgccag gcacctatgg cggcattatc
    11401 tttttgattt caaaattctt tactgcgttt accgatatgg gtaccggcat tatgttggat
    11461 tcccgacgca agatcggtcc aaaaggtaag ttccgtcctt ttattctgta tgcgtcattc
    11521 ccggtcacct tactggcgat cgccaacttt gtcggcacac cgtttgatgt caccggtaaa
    11581 acggtgatgg ccactattct gtttatgctt tacggactgt ttttcagcat gatgaactgc
    11641 tcctacggcg caatggtccc ggcaatcacc aaaaacccca acgaacgcgc ctcactggcg
    11701 gcatggcgtc agggcggcgc tacgctgggc ctgctgctgt gcacggtggg attcgtgcca
    11761 gttatgaatc ttatcgaagg taatcagaaa cttggctata tcttcgccgc cacgctgttt
    11821 tcactgttcg gcctgctgtt tatgtggatc tgctactcag gcgtgaaaga gcgttatgtc
    11881 gaaacccaac caaccaatcc ggcgcaaaag cctggcctgt tgcaatcttt ccgcgcaatt
    11941 gccggtaacc gcccactgtt cattctgtgt attgccaacc tctgcacctt aggggcgttt
    12001 aacgtcaagc tcgccattca ggtctattac acccagtacg tacttaacga tcccatcctg
    12061 ttgtcgtata tgggattttt cagcatgggc tgtattttca tcggcgtgtt cctgatgcct
    12121 ggcgcagtca gacgttttgg taagaagaag gtctatatcg gcggcctgct gatttgggtg
    12181 ctgggcgatc tgctcaacta tttcttcggc ggcggctcgg tcagctttgt ggcgttctcc
    12241 tgcctggcgt tcttcggctc agcgcctgaa ctgggcgctg gtttccgaca ccgtcgagta
    12301 cggcgagtgg cgcaccggcg tgcgttcgga aggcacggtc tacaccggct tcaccttctt
    12361 tcgcaaagtg tctcaggcgc tggctggttt cttccccggc tggatgctga cgcaaattgg
    12421 ctatgtgccg aacgtcgcac aggctgacca caccattgaa gggttacgcc agttgatctt
    12481 catctaccca agcgcactgg cggtagtcac cattgtggcg atgggttgct tctacagcct
    12541 gaacgagaag atgtatgtcc gcattgtgga agagatagaa gcccgtaaac gcacggcgta
    12601 ataataataa acgacgccct gcggggcgtt ataaggagtg attatgtctg accataatcc
    12661 actgacatta aaactgaatc tgcgggaaaa aatcgcctat ggtatgggcg acgtcggttc
    12721 gaatttaatg ctctgcatcg gtactctgta tctcctcaaa ctttataccg atgaattagg
    12781 gatgcctgct tactatggcg ggattatctt tctggtcgcg aagtttttta ccgcgtttac
    12841 cgatatgctc accggatttt tactcgactc gcggaaaaat attgggccta aaggcaaatt
    12901 tcgtcccttt attttatatg cagcagttcc ggcggcgtta attgcaacgc tccagtttat
    12961 cgccaccacc ttttgtttgc cggttaaaac gacgattgcc accacgctgt ttatgatgtt
    13021 tggactttca tacagtctga tgaactgctc gtatggtgcg atgatcccgg caattaccaa
    13081 aaacccgaat gagcgcgcgc agctcgcggc ttaccgccag ggtggtgcaa ccatagggct
    13141 attgatttgt accgtggcgt ttattccgtt gcagtcgctt ttttctgact caaccgtcgg
    13201 ttatgcctgt gcggcactta tgttctccat tggcggcttt atttttatga tgctgtgcta
    13261 cagaggcgtc aaagagcatt atgtggacac agcgccaacc ggacataaag ccagtattct
    13321 caaatctttt tgcgcgatat ttcgtaatcc accattgctg gttttatgca ttgctaacct
    13381 gtgtactctg gcggcattta atatcaaact ggcgattcag gtctattaca cccagtatgt
    13441 gctgaatgat attaatttat tgtcgtggat gggattcttc agtatgggat gcatcctcgt
    13501 cggcgtatta ctggtgccag taactgtaaa atgttttggt aaaaaacagg tttatttagc
    13561 tggcatggtg ctatgggcgg tgggtgatat actgaattat ttctggggaa gtaactcttt
    13621 cactttcgtc atgttctctt gtgtcgcctt ttttggcacg gcctttgtaa acagcctgaa
    13681 ctgggcactg gtaccagata ccgtagatta cggtgaatgg aaaaccggta ttcgtgccga
    13741 aggttctgtt tataccggtt ataccttctt tcgtaaaatt tctgccgcac ttgctggctt
    13801 cttgccaggc attatgctga cgcaaattgg ttatgttccc aacatcgcgc aaagcgatgc
    13861 gacattacag ggtttgcgtc agctcatttt tatctggcct tgtgcactgg caattattgc
    13921 tgcattgacg atgggattct tttacacact caatgaaaaa cggtttgcat tgattattga
    13981 ggaaatcaac caacgcaaaa ataaagagat ggcgacagaa aaaaaaacgg cttccgtaac
    14041 gttataaatg tcaatagccg ctatttccat cctggtgggt ggcggcctcc ctacgtttaa
    14101 aaaatggact tattatgaaa aagattaatg caataatttt attatcttct ctgacctctg
    14161 cctcggtatt tgctggc
//
LOCUS       DAJTRH010000092        13890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTRH010000092 DAJTRH010000000
VERSION     DAJTRH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..2635
                     /locus_tag="OO339_003111"
                     /pseudo
     CDS             1..2635
                     /locus_tag="OO339_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2645..6291
                     /locus_tag="OO339_003112"
                     /pseudo
     CDS             2645..6291
                     /locus_tag="OO339_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6352..6759
                     /locus_tag="OO339_003113"
     CDS             6352..6759
                     /locus_tag="OO339_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927646.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7301..8389
                     /locus_tag="OO339_003114"
     CDS             7301..8389
                     /locus_tag="OO339_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5927647.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8400..10679
                     /locus_tag="OO339_003115"
     CDS             8400..10679
                     /locus_tag="OO339_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927648.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10672..11808
                     /locus_tag="OO339_003116"
     CDS             10672..11808
                     /locus_tag="OO339_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5927649.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            11805..13808
                     /locus_tag="OO339_003117"
     CDS             11805..13808
                     /locus_tag="OO339_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927650.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3418 a         3663 c         3567 g         3242 t
ORIGIN      
        1 ccccgatttg ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat
       61 catccgttcg cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat
      121 cgaatgacga aagcctgcgc tgcgttccca cacgcagcac tggcagcact ggcggaactt
      181 ctagtgcaaa aagaagagaa tagttggcgc attatgctaa tgacgatgtt tatctcgcaa
      241 ccagcactgg cagaacaggt cattccctgg ctttcgacac ccgcagttgc agtgctgaaa
      301 tcatgccagc accaactgac acagccctca aaccatgcca gcgccgatct actgccagcc
      361 atagtagtct ccccaccatg gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat
      421 ttagcgccat taggcatcga gccaatctgt tatctgacag aagaaatcag taatcaactt
      481 ttggcgaaat atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc
      541 aacctgttgg caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc
      601 gaagcggtag ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag
      661 gtgtttcatt cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg
      721 gaggctggag aaagcagtaa aagcatggaa tgcccttact ctatctccac ataccgatac
      781 cgaatacgcc atgttacatt ttggactcaa aggtttacct ggtttagtta actcaattac
      841 acgctatcca caggaagcct tgcccatcac gaattacttc gcagcgagtg agctggctcc
      901 tgccgtcgcc cgtgccttca acaaactcaa aacgctacgc gaaaacgccc gcacctggct
      961 gttgaaatac ccggaacatg cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg
     1021 tgaagcacag gataacgccc gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc
     1081 attactgcaa gaaatcgccc ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc
     1141 tctgcttgcg ctcgatccct tagataatca cccgacaaaa atccccactc ttccgacctt
     1201 ttatcagcca tcgatctgga cgcgcccggt attaaaagca aacgcccaat cactgccaga
     1261 taacgccctc ctccacctcg gcgaaatgct ccgcttccct caggaagagg ctctgtatcc
     1321 gggattattg caggtgaaag acgcctgtac cgctgactct ctggctgaat ttgcctggga
     1381 tctgtttacc gcctggcaga ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc
     1441 gttaggcgtt ctcggtaacg atgacaccgc ccgcaaactg acgccattaa tacgcgcctg
     1501 gcctggtgaa tcccagcata aacgcgccac cgttgggttg gatattctcg ccgctatcgg
     1561 tagtgatatc gccctgatgc agcttaacgg cattgctcag aaactgaaat tcaaagcatt
     1621 acaggagcgg gcaaaagaaa agattgccga cattgccgag agccgcgaac tcacggtagc
     1681 ggagcttgaa gatcggttag caccggatct cggtctggat gataacggtt cgttgctgct
     1741 ggattttggc ccacgccagt tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg
     1801 tgatgtttcc ggcagccgcc tgaaagacct gccaaaacca aacaaaagcg attatgagtc
     1861 acaggcgaac gatgctgtta accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc
     1921 ggcccagcag gtagcaaggc ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga
     1981 aaacttccaa ctcttcctgg ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat
     2041 ttggggcgtt tatagcgccg acaaccagct actggcctgt ttccgggtag cggaagataa
     2101 cagctacagc accgctgacg atgatctttt caccctgccg gaaggcgata tctctatcgg
     2161 cattcctcac gttctggaaa tatcaccgac ggatgctgtc gcctttggtc agctttttgc
     2221 cgactacgaa ctgctaccac cgttccgtca gcttgaccgt aacagttacg ccctgacaga
     2281 agccgagcgc aacgccagcg aactgacccg ctgggcaggc agaaaatgcc cgagcggccg
     2341 ggtcatgggg ctggcaaata aaggctggat aaagggcgaa ccacaggatg gaggctggat
     2401 gatcaaacct ctcggtcgct ggtcgttaat catggaaatc gacgaaggtt ttgcggtggg
     2461 tatatcacca gccgaactta gcgctgagca gctcttaagc aagctgtggc tatgggaagg
     2521 caaagcggaa agctatggct gggggagtaa ttcaacacag gaagcgcagt tctccgtact
     2581 cgatgccatc accgccagcg agctaattaa cgatattgaa gccctgtttg aataaggaaa
     2641 gagcatggac aaggaattac cgtggctggc ggataacgcc caactggaac tgaaatataa
     2701 aaaaggcaaa acaccactca gtcatcgcaa ttggccgggc gaaccagtgc ctgttatcac
     2761 tgaaagcatc attcagacac tgggtgatga attgctacaa aaagctgaga agaaaaaaaa
     2821 cattgtctgg cgttatgaaa atttttcacc ggagtggcag tccgccatta cgcaggccat
     2881 caacttgatc ggcgaacaca aaccctcagt cccggcccgc acaatggcgg cactagcctt
     2941 tatcgcgcaa aatgacaacc aacagttgct cgacgaaatc gtccaacaag aggggctgga
     3001 atatgcgact gaggtggtga ttgcacgcca gtttattgca cggtgttatg agagtgatcc
     3061 tctggtagtc acattgcagt atcagaacga ggactatggc tatggttatc gctcagaaac
     3121 ctataacgaa ttcgatctcc gactgcgtaa acacctctct ctggcagagg aaagctgctg
     3181 gcagcgttgc gccgacaaac tcattgccgc actgccagga ataaccaaag ttcgccgccc
     3241 ttttattgcg ctgatcctcc ccgaaaaacc agaaatcgcc aatgagttgg taagccttga
     3301 atgcccgcgg actcattttc attctaagga gtggttaaaa gttgttgcta ctgaccccaa
     3361 agcggtgaga aaactcgaac gctactggag ccaggatata tttagcgatc gagaagccag
     3421 ctacatgtcg catgaaaacc acttcggcta cgcggcctgc gctgcccttt tgcgcgaaca
     3481 aggactggca gtcgttccgc gcctcgcgat atatgcccat aaagaagatt gcggcagtct
     3541 gctggtacaa attaaccatc cgcaagtcat ccgcaccttg ctgttagttg ctgataaaaa
     3601 caaacccagc ctgcaacgtg tggctaaata cagtaaaaac ttcccccatg cgacactcgc
     3661 cgcactggca gaactgctgg cgttaaaaga accaccagcc cgcccaggtt atccaatcat
     3721 cgaagacaaa aagctgcctg cacagcaaaa agctcgagat gaatactggc gtacgctgtt
     3781 acaaacgctg atggcatcgc agccacaact ggcagcagaa gtgatgccgt ggttaagtac
     3841 tcaagcccgg gcagtgctga atagttattt atcggcaccg cccaaaccag ttattgatag
     3901 caccgataac agccaaatgc ctgaaatcct cgtttcacca ccatggcgtg ggagaaagaa
     3961 aaagacttcc atacgtcact tttcgctgcc tgatcttacg cttgcaccgc aaacgtacag
     4021 gtcgccgaat aaacactgga cgccggaaca gcaaactgca attgaccatt ttagcgccat
     4081 gtcttttgac gaacgtcttg ctacgagagg agcggatatc ttcttgcggg aattaggttt
     4141 tgacgttcat agcctggaat ttaataagcg tcttcttaat ggacagttgg atgcctttga
     4201 tgctcagcaa ctgcaagaat acacatcaat aggactattg cgtacttatc acgcgcatca
     4261 ggccaaatct aaacagtacc gggaagacgc caccaatgca ttactggcac aagatagcat
     4321 tgcgttgatg ggagggtggc gaacttttca gcacggattt tactatggta atgaacagcg
     4381 ttgggggatt tggaatctgt accttatcgc gcagatgccg cgcgaaatgg cggtatcgtg
     4441 ctggcaacaa attgtcgagg ctgattttca ttacaccggg gtggaatatt tgttaagtgt
     4501 gctgggtacc gatgctttgc ctggattaaa caccgccttt gcccgacatc caaaagagat
     4561 atttccgctg ctgattcatt tcggcgcaac agaactggcc ctgcccattg cccgcgtctg
     4621 gcaccttttt gcgggccagc gtaatctggc tcgccagtgg attttacaat ggccggaaca
     4681 tacggctact gcacttatcc ctcttgtctt caccaaatct agcgataaaa gcgaagctgc
     4741 attacttgcc ctgcgtttac tttacgaaca tgggcatggc gaatggctac aaactgtagc
     4801 caatcgttgg cagcgtacag atctatggcc tgccctggaa caattactta aacagagtcc
     4861 aatagagatt tacccggcac gcattccaaa agcccctgat ttctggcatc cgcaaatgtg
     4921 gtccaggccg cgccttatca ctaataatca acctgttacc gatgacgctc tggaaattat
     4981 cggcgaaatg ctgcgcttta cccagggggg acgtttttat agcgggctgg aacaactgaa
     5041 aacgttctgc cagccacaaa cgctggcagc ttttgcctgg gatctcttca ctgcgtggca
     5101 acaagctggt gcccccgcaa aagacaactg ggcatttctg gcgttaagtc tctttggtga
     5161 cgaaagcacg gcacgggatc taacgacaca tatcctcgcc tggccacaag aaggcaaatc
     5221 tgcccgtgcg gtcagtggca tgaacatcct taccctgatg aataatgata tggcgctgat
     5281 gcagctgcat catatatcgc aacgggcgaa atcccgcccc ttacgtgata acgcggcgga
     5341 atttcttcag gtagtcgcag aaaatcgcgg gctaagccag gaagagttag cggacagatt
     5401 agtcccaaca ctgggcctta atgatccgca ggcgttgagt tttgattttg gtccccggca
     5461 gtttaccgtt cgcttcgatg aaaacctcaa cccggttatc tttgatcagc aaaacgttcg
     5521 ccagaaaagt gttccccggt tgcgcgccga tgacgatcaa ctgaaagcgc ccgaggcact
     5581 ggcccgacta aaagggttaa aaaaagatgc tactcaggtg agcaaaaacc tgctcccgcg
     5641 tcttgaagct gccctacgta ccacccgacg ctggtcgctg gcagattttc attctctgtt
     5701 tgttaatcat ccctttaccc gtctggttac ccagcgatta atatgggggg ttatccggca
     5761 aatgaaccgc gtcgtttact caacgccttt cgtgtggccg cagaggggga gttctgcaat
     5821 gcgcaagatg agccaattga cctgcctgcg gacgctctga ttggcattgc ccacccgtta
     5881 gaaatgacag cagaaatgcg cagtgaattt gcacagcttt ttgccgatta cgaaattatg
     5941 ccgccttttc gccagttgtc gcgccgcacg gtgctgctca cacctgacga gtcaaccagt
     6001 aacagcctga ctcgctggga aggtaaatcc gctaccgttg ggcaacttat gggaatgcga
     6061 tacaaaggct gggagtcagg ttatgaggac gcatttgtct atgacctggg tgagtaccgg
     6121 ctggtcctta agttttcacc cggttttaac cactacaatg ttgatagcaa agcgctaatg
     6181 agcttccgtt ctcttcgagt gtaccgtgac aataaatccg tcacttttgc cgaacttgat
     6241 gtgtttgatt tgagtgaggc gttaagcgca cctgacgtca ttttccatta aaacaaagcg
     6301 ggcagtgatg cgactgcccg ttatctacac gacttaccag cggggaaagc gatgatcgtg
     6361 caaaaagagc tggttgctat ttacgattat gaggtccctg tacctgaaga tccgttttcc
     6421 ttcagacttg agatccataa atgctctgaa ttatttacag gttccgtcta ccgactggaa
     6481 cggttccggc tacgtccaac atttcatcaa cgtgatcgag aggatgttga cccgctaata
     6541 aatgatgcgt tgatttatat aagagatgag tttattgatg agcaaaaatt acgaggcgaa
     6601 tcacctgaac ctgtaatagt aatttttaat tgtaaactac agaatatatt caaccaacaa
     6661 atagaattat atactctaga taattcaagt cgcagcacat gcaacttgaa gtatgacgag
     6721 tatagcttta ttaaaggggg gaacggtatt cccccttaga tgcgtttcaa tttgcccgtt
     6781 acttcaacaa atcaatcaac cgtaacagag ccactaatag ctccagtaca gcaatgagca
     6841 cggttaactt ttgtgtcagc gtgtccatcc gctctcctta ttaaggagca caacaccagc
     6901 accttaccct tacactgcct gtcgatactg gtttacgttt gttgagtcgc catgttgcac
     6961 tccggatcag agcactacct gcggcaccac ccgtccggta gccaggactt gaaataggtt
     7021 cgatccggcg caacttccca acagtcagga ttataaatct cattatctta attgcaacac
     7081 tgtccagagg tggttataat tcgttcgttg gaacacgatg ttgcacaggc agtggtgtta
     7141 ggcctgaaaa tagtaaataa aagaacctgc ctcaccagca gtttttttta tttactgtga
     7201 tctgctttcc agatattttt cgctcaaaca actaatgcgc caaacattta ttgcgcgtaa
     7261 aatatcgttt atttcattaa tacatttcag ggatgaatat atgtcaccgc agaataatca
     7321 tctgcagcgt ccgcctgctg ctgtgttata cgccgatgaa ctggcaaaat taaaacaaaa
     7381 tgataacgca ccttgcccgc ccggttggca gttaagtttg cctgcggccc gtgcttttat
     7441 ccttggcgat agcgcgcaaa atattagccg taaagtggtg attagtccct ccgctgtcga
     7501 acgtatgtta gtcactcttg ccaccggacg cggtttgatg ttggtggggg aacctggcac
     7561 cgcaaaatct ctcctgtctg aattactggc aacctctatt agcggcgatg ccgggttaac
     7621 catccagggc ggcgcatcta ctaccgaaga tcaaatcaag tacggctgga actacgccct
     7681 gcttatcaat catggtccat caacggaagc gctggtccct gccccgcttt accagggaat
     7741 gcgcgacggc aaaatcgtcc gttttgaaga gataacgcgt acaccgctgg aagtgcaaga
     7801 ctgtctgtta ggtatgcttt ctgacagggt gatgacggtg ccggaactca ctggtgaagc
     7861 cagtcagctc tatgcgcgag aaggctttaa tattattgcc actgccaata cccgcgatcg
     7921 cggcgtcaat gaaatgagtg ctgcgctaaa gcgtcgcttc gattttgaaa ctgtctttcc
     7981 gattatggat ttcgcccagg agctggagct tgtcgccagt gcttcggcgc gtttgctggc
     8041 ccatagcggt attccgcata aagtgcctga cgcggtactg gagttactgg tccgcacctt
     8101 ccgcgatctg cgtgccaacg gcgaaaagaa aacgtcaatg gatacactaa cagcgattat
     8161 gtccaccgct gaagccgtga acgtggccca cgccgtaggc gtccgcgcct ggtttttagc
     8221 gaatcgcgca ggtgagccag ccgacctggt ggactgcatt gccggaacca tcgtcaaaga
     8281 taacgaagaa gatcgcgctc gtctgcgccg ttacttcgaa cagcgtgtcg ccacacataa
     8341 agaagctcac tggcaggctt attatcaagc ccgccaccgc ctgccgtgag gaaagatgca
     8401 tgagcgagcc gttaattgtc ggcatccggc atcatagtcc ggcctgcgcc cggctggtga
     8461 aatcgttaat cgaaagccag cggccacgat acgtgttgat tgaaggccct gctgatttta
     8521 atgaccgggt agacgaactg tttttagccc accagcttcc ggtagctatt tacagttatt
     8581 gccagtatca ggacggtgca gcccccgggc gtggtgcctg gacgccattt gctgaatttt
     8641 cgccggagtg gcaggcgcta caagccgatc gtcgtattca ggcacaaact tacttcatcg
     8701 atttgccttg ctgggcgcag agtgaagaag aggacgattc gcctgatacg caagaggaaa
     8761 gccagacctt gctgctgcgt gccacccgca tggataacag cgacaccctg tgggatcact
     8821 tgttcgaaga tgaaagccag caaactgcat taccctctgc actggcgcac tattttgccc
     8881 aactgcgggg cgactccccc ggcgatgctc taaatcgtca gcgcgaagcc tttatggccc
     8941 gctggattgg atgggcgatg cagcaaaata atggcgacgt gttagttgtc tgcggtggct
     9001 ggcacgctcc ggcactggca aaaatgtggc gcgaatgtcc gcaggtaatt aacacgccag
     9061 aattgccgtc gctggcagat gccgttacag gttgttatct caccccctac agtgaaaagc
     9121 gccttgatgt gctggcagga tacctttcag gaatgcctgc cccggtctgg caaaactggt
     9181 gctggcagtg gggcttacag caggcgggag aacaactgct aaaaacggtt ctcacccgtt
     9241 tgcgccagca caagttgcct gcttcgaccg cggatatggc tgccgctcat ctgcatgcga
     9301 tggcactggc acagttgcgc ggtcatacac taccgttacg cactgactgg ctggatgcca
     9361 tagcaggatc gctgattaaa gaagccctga atgcgccgtt gccgtggagc tatcgcggcg
     9421 ttattcatcc cgataccgat ccgattctgc taacgttgat agacacatta gcgggtgacg
     9481 gattcggtaa acttgcccct tctacgccac aaccgcctct gccaaaagat gtcacttgcg
     9541 aactggaacg taccgcaatc tctcttccgg cggagcttac cttaaatcgc tttaacccca
     9601 atggactagc gcaaagtcag gtgttacatc ggctggcaat actggagatc ccagggattg
     9661 tacgccagca gggaagcaca ctgacacttg caggcaacgg tgaagaacgc tggaaattaa
     9721 cccgcccgct tagccagcat gcggcattga ttgaggccgc ctgctttggt gccacactcc
     9781 tggaagccgc acgccataaa ttagaagccg atatgctgga cgcgggtgga atcggcagca
     9841 ttaccacatg tcttagccaa gcggcgtcag cgggtctggc gtccttcagt caacaattac
     9901 tggagcaact cacattatta atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc
     9961 tggaagtgct atatgcctta tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga
    10021 tattacaaac gacgttatgc gcggctatcg atcgcacgct gtggctgtgt gaatccaacg
    10081 gcagaccgga tgaaaaggag tttcacgctc acttgcatag ctggcaagcg ttgtgtcata
    10141 tcctgcgcga tctacatagc ggtgtgcaac tccccggcgt ttcgctttct gcggcggtag
    10201 ccttgctgga gcgacacagt caggcgattc atgtcctggc actggatcgc ggcgcgactc
    10261 ttggtgcgct gatgcgtctg gaacatccca acgctagtgc cgaagcggcg ctgacgatgc
    10321 tggcgcagtt atccccggca caatccggcg aggcgctgca cggtttgctg gcattagccc
    10381 gtcatcaact ggcctgtcag ccggcattta tcgccggttt cagcagtcat ttaaatcaac
    10441 taagtgatgc cgattttacc aatgccctgc ccgatttacg cgcggcaatg gcctggctac
    10501 cgccacgaga acgcgggacg ctggcgcatc aggtgcttga gcattatcaa ctggcgcaac
    10561 ttcccgtttc ggcactgcaa atgccgttgc attgtccacc gcaagccatt gcacatcatc
    10621 aacaactcga acagcaggca ctggcatcgc tgcaacactg gggagttttc catgtctgaa
    10681 ctgaacgatc ttctgaccac ccgtgagcta caacgctggc gattaattct tggcgaagcg
    10741 gcagaaacga cgctttgtgg gctggatgac aacgcccggc agatagatca cgcgctggag
    10801 tggctgtatg ggcgcgatcc tgaacggctc cagcgtggtg aacgctctag tggattaggt
    10861 ggctcaaatc tcaccactcc tgagtggatc aacagtattc acacgctgtt tccgcaacag
    10921 gtgattgagc ggctggaaag cgatgccgta ctgcgctacg gcattgaaga tgtggtgaca
    10981 aatctcgacg tgctggaacg tatgcagcct tccgaaagcc tgctacgcgc cgttttgcac
    11041 accaaacatc tgatgaaccc cgaagtactg gctgccgccc gcaggattgt gcgccaggtt
    11101 gtggaagaaa ttatggcgcg actggcaaag gaagttcgtc aggctttttc tggtgtccgc
    11161 gatcgtcgtc gccgctcatt tattccactg gcgcgaaact ttgatttcaa aagtactctg
    11221 cgcgccaacc tgcaacactg gcacccgcaa cacggcaagt tgtatatcga attcccccgc
    11281 tttaacagcc gcattaaacg ccaaagcgag caatggcaac tggtcttact ggttgatcaa
    11341 agcggatcga tggtcgattc ggtgatccac tctgcggtga tggcggcctg tttatggcag
    11401 ttacccggca ttcgtaccca tctggtggcg tttgacacaa gcgtcgttga tctcacggca
    11461 gacgttgccg atccggtaga gttattaatg aaagtgcaat taggcggcgg aaccaatatc
    11521 gccagtgcca tggagtatgg tcggcaactt attgaacaac ctgcaaaaag cgtcattatc
    11581 ctcgtgagcg atttttatga agggggttca tcatcattgc tgacgcacca agtgaaaaag
    11641 tgtgtccaga gcggcatcaa agtgctggga ctggcagcgc tcgatagcac agcaacacct
    11701 tgctatgacc gcgatacggc ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg
    11761 acaccgggcg agctggcatc atggcttgcg gagaatcttc agtcatgaat tcactacgtc
    11821 cggaattatt agaactgaca ccgcaggcac tgacggcgtt aagcaatgcc ggttttgtta
    11881 agcgcagcct taaggaactg gaaaatggca acgtcccgga aatcagccat gagaacagcg
    11941 ctttaatcgc caccttcagt gacggtgtcc gtacccagct ggcgaacggc caggcactga
    12001 aagaggctca gtgcacctat ggggccagcg gcatgtgccg tcatcgcgtg atgctggtgt
    12061 taagttatca acgactttgt gctaccgccc agcctacggg aaaagaagag gaatgggatc
    12121 cggcaatctg gctggaagaa ctggctaccc ttcccgatgc cacccgcaaa cgcgcacagg
    12181 cgctggtcgc taaaagcatc accattgagt tgttctgtgc gccgggtgaa attccctctg
    12241 cccgcttacc gatgagcgat gtgcgttttt attcccgcag cagtattcgt ttcgcccgtt
    12301 gtgattgtat tgaaggcaca ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc
    12361 aggcaaaagc gcagcaagcg gaattgactc atttaatctg gcagatgcgc agcgaacacg
    12421 tcacatcatc tgacgatccg tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc
    12481 aacaactaag ccaggcatta tggctgagcg gcatcagcca gccgcttatt cactacgagg
    12541 ccgctttcag ccgcgcgcag caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat
    12601 cgctacggca actgcgcgcc agcgttgatg ccttccacgc ccgcgccagc cactatcatg
    12661 ccggagaatg cttacgtcag cttgcggcat taaacagtcg attaaattgc gcacaagaga
    12721 tggcccggag cgacagtgtt ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct
    12781 ctggcattgc cggagaagca aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt
    12841 gctggcagga tattgagcat tatggtttac gcatctggtt taccgatccc gacaccggca
    12901 gtattttgca cctttcgcgc agttggccgc gaagtgaaca ggaagactca ccggcagcta
    12961 cgcgtcggct gtttagtttt caggctggcg cactggcggg tgggcaaatt gtttcacaag
    13021 cagcaaaacg cagtgccgat ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg
    13081 ttgtaccgct gtcgcctgat gcctggcaaa tgttgagcgc gccgttacgc cagccgggca
    13141 ttgtggcttt gcgggaatat ttacgccagc gtccccccgc ctgcatacgg cctcttaatc
    13201 aggtcgataa cttatttatt ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca
    13261 gccgccagac gctggatgcg caggtaatca gcggcgaagg tgaagataat ctgctgacgt
    13321 tatcactgcc agcctcagcc agcgcgcctt atgccattga acgcatggcg gcgcttttgc
    13381 aacaaacaga cgaccccgtg tgtctggttt ctggctttgt cagttttgtt gatgggcaat
    13441 tgacactgga accacaggta atgatgacaa agactcgtgc ctgggcgctg gacgcagaaa
    13501 ctgcgcctgt ggtggtatca ctaccttctg ccagcgttct gcctgtgccg tctactgctc
    13561 atcagttgct gatgcgctgc caggcgttac ttattcaact gctccataac ggctggcgct
    13621 atcaggaaca gagcgctatt aatcaggcag agttgctggc gaatgacctc accgcggtgg
    13681 gtttttatcg gctggcacat gtgttggcac aatttcgtaa tacagaaagc gaggcacggg
    13741 tagaagcaat gaataacggt gttttgcttt gcgaacaatt attccccatg cttcagcaac
    13801 aaggatgaaa tagtgctttt tactaagagt tctactccag ttccggactg ctcacgccac
    13861 ggtattaggc atatcctata tagcccctgg
//
LOCUS       DAJTRH010000093        13636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTRH010000093 DAJTRH010000000
VERSION     DAJTRH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..1713
                     /gene="ldtD"
                     /locus_tag="OO339_003118"
     CDS             <1..1713
                     /gene="ldtD"
                     /locus_tag="OO339_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5927651.1"
                     /translation="EQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPV
                     YLNQLQLLYAARDMQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGM
                     ARDVVLSDAMMGYLHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPT
                     FVAGLAPQHPQYAAMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTG
                     MLDGGPKITLPGDDTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNEL
                     VEAVKRFQAWQGLGADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMV
                     NIPAYSLLYYQNGNQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDIL
                     PKVRNDPGYLESHGYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGR
                     YKFNMPSSEAIYLHDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRIS
                     DALKQGDTRYVNIRQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAE
                     QLIR"
     gene            1894..2442
                     /locus_tag="OO339_003119"
     CDS             1894..2442
                     /locus_tag="OO339_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5927652.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2469..3116
                     /gene="gloC"
                     /locus_tag="OO339_003120"
     CDS             2469..3116
                     /gene="gloC"
                     /locus_tag="OO339_003120"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5927653.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3339..4529)
                     /gene="aspC"
                     /locus_tag="OO339_003121"
     CDS             complement(3339..4529)
                     /gene="aspC"
                     /locus_tag="OO339_003121"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5927654.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(4714..5802)
                     /gene="ompF"
                     /locus_tag="OO339_003122"
     CDS             complement(4714..5802)
                     /gene="ompF"
                     /locus_tag="OO339_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5927655.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6403..7803)
                     /gene="asnS"
                     /locus_tag="OO339_003123"
     CDS             complement(6403..7803)
                     /gene="asnS"
                     /locus_tag="OO339_003123"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5927656.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(7972..9174)
                     /gene="pncB"
                     /locus_tag="OO339_003124"
     CDS             complement(7972..9174)
                     /gene="pncB"
                     /locus_tag="OO339_003124"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5927657.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKQGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9440..12055
                     /gene="pepN"
                     /locus_tag="OO339_003125"
     CDS             9440..12055
                     /gene="pepN"
                     /locus_tag="OO339_003125"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5927658.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12262..13029)
                     /gene="ssuB"
                     /locus_tag="OO339_003126"
     CDS             complement(12262..13029)
                     /gene="ssuB"
                     /locus_tag="OO339_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5927659.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13026..>13636)
                     /gene="ssuC"
                     /locus_tag="OO339_003127"
     CDS             complement(13026..>13636)
                     /gene="ssuC"
                     /locus_tag="OO339_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5927660.1"
                     /translation="LAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQMLR
                     NVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYGL
                     SGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQT
                     DVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3469 a         3496 c         3440 g         3231 t
ORIGIN      
        1 gaacagggcg aggcactgcc gcaggcgcaa gccacggcaa taatggcggg gatccagcca
       61 ttgcctgaag gtgcggcaga aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt
      121 tacaagccgg tttatcttaa ccagcttcaa ctgttgtatg ccgcacgcga tatgcaaccc
      181 atgtgggaaa accgtgatgc tgttaaagcc ttccagcaac agctggcaga ggtggcgatt
      241 gctggtttcc agccgcagtt taataaatgg gtagagttac tgaccgatcc tggtgttaac
      301 gggatggcac gtgacgtggt gctctctgat gcgatgatgg gctatctcca tttcattgca
      361 aatattccga tcaaaggcac tcgctggcta tatagcagta aaccttatgc gctttcaacg
      421 ccgccgcttt cggtgattaa ccaatggcag ctagcgctgg ataaaggtca attgcctacg
      481 tttgttgcag gactggcacc gcagcatccg caatatgcgg cgatgcatga atcgttactg
      541 gtcttactca gtgacaccaa accgtggccc caactgaccg gcaaagcaac gttgcgccca
      601 gggcagtgga gtaacgacgt accggcgttg cgcgaaatat tgcaacgcac aggcatgttg
      661 gacggggggc cgaaaattac tctacctggc gatgacacgc caactgacgc ggtagtcagc
      721 ccatccgctg ttactgttga aacagcagaa actaagccga tggacaagca aacgacgtct
      781 cgtagtaaac ctgcgcctgc cgttcgtgcc gcctacgata atgaactggt ggaagccgtt
      841 aaacgttttc aggcatggca aggattggga gcagatggtg ctattggccc ggcaacgcgt
      901 gactggttaa acgtaacgcc cgcccagcgt gctggcgtgt tggcactcaa catccagcga
      961 ttgcgcttgc tgccaacaga gctttctacc gggatcatgg ttaacattcc agcctattcg
     1021 ctgctctact atcagaacgg caatcaggtg ctggattcgc gagtcattgt cggtcgcccc
     1081 gatcgcaaaa cgccgatgat gagcagtgca cttaacaacg tagtggtaaa cccgccgtgg
     1141 aacgtaccac caactctggc acgcaaagat attctgccga aagtgcgcaa cgatccggga
     1201 tatctcgaaa gccatggcta tacggtgatg cgcggctgga acagcagaga agcgattgac
     1261 ccatgggagg ttgactggtc tacaatcacg gcctcgaatt taccgtttcg cttccagcag
     1321 gctccaggcc cacggaactc gctggggcgc tataaattca atatgccgag ttcagaggcc
     1381 atttatttgc atgacacgcc gaaccacaat ctgttcaagc gtgatacacg cgcattgagc
     1441 tcaggctgtg tacgagtgaa taaagcttcc gatctggcga atatgctgtt gcaggatgca
     1501 ggatggaatg acaaacgtat ttctgatgcg ctgaagcagg gtgatacacg ttacgtcaat
     1561 attcggcagt cgattccggt gaatctctac tacctgacgg cctttgttgg tgcagatgat
     1621 cgtacccagt atcgtacaga tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa
     1681 atcgtatcga aagcggaaca attaatcagg taaatgaagt agttcgggga aatcaattgt
     1741 cgtaataatt agcatgattg ggggcgattc tctgcagccc ccgtcactgc tggggttgag
     1801 tcatcttgac gtctgcttta cgggcggtta aggtgcctct tgtgcgccag aagtgcatat
     1861 aaacgataac attgagctgt agacttgatt atcatggaca aattcgacgc taatcgccgc
     1921 aaattgctgg cgcttggtgg cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt
     1981 gcaacactct ctaccccacg cccgcgcatt ttgacactca ataatcttca taccggagag
     2041 tcaatcaaag cggagttttt cgatggcaga ggctatattc aggaagaatt ggcaaaactt
     2101 aaccattttt tccgcgatta ccgcgcgaac aaaataaagt ccatcgaccc aggattattc
     2161 gaccagttgt atcgcctgca agggttgtta ggcacgcgca aaccggtgca actcatttcc
     2221 ggttatcgtt ccattgatac caacaatgaa ctacgcgccc gcagccgtgg agtagcgaag
     2281 aaaagctatc acactaaagg ccaggcgatg gatttccata ttgaaggtat cgcgttaagc
     2341 aatattcgca aagccgcgtt atctatgcgc gcaggtggtg taggatatta cccacgtagt
     2401 aactttgtgc atattgatac cgggccagca cggcactggt agtaatcgct taacgaaaca
     2461 ggggcagtat gaactatcgt attattccgg tcaccgcatt ctcccagaac tgttcattga
     2521 tctggtgtga acaaacccgc ctggccgcac tggtcgatcc tggcggcgat gcggaaaaaa
     2581 tcaaacagga agttgatgac agcggcctga cactgatgca gatcctgctg acgcatggtc
     2641 atctggacca cgttggcgca gcggcggaac tggcgcaaca ttacggcgtg ccggtttttg
     2701 gcccggaaaa agaagatgag ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg
     2761 gtctggaaga gtgccagccg ctgacgccag atcgttggct gaacgaaggc gataccatca
     2821 gcatagggaa tgtgacttta caggtgttac attgccctgg gcatacgccg ggtcatgtcg
     2881 tgttttttga tgatcgggca aagctgctga tttctggcga tgttattttc aaaggcggag
     2941 tagggcgcag tgacttcccg cgtggcgatc ataatcaact gatttcttca atcaaagata
     3001 aattgctgcc actgggggat gacgtgacat ttattccggg tcacggacca ttatctacac
     3061 ttggttatga gcgcctgcac aatcccttcc tgcaagacga aatgcccgtc tggtaaaggc
     3121 gcataaaaaa gtccactttt aatgcgggcc tggatttctg gcaaagtgcg ctttgtttat
     3181 gccggatgcg gcacgagcgc cttatccggc ctacaaaatc gtgcaaattc aaaatattgc
     3241 aggggacgcg taggcctgat aagcgtagcg catcaggcaa tgttgcgttt gtcatcagtc
     3301 tcagcccgct tttcagcggg cttcattgtt tttaacgctt acagcactgc cacaatcgat
     3361 tcgcacagcg gagccatgtt atctggtgtc atcccggcca catttacgcg accagaagca
     3421 acagcatata cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt cagaccactg
     3481 aaggagaaca tgccgttctg tttgatgata aagctgaagt cgcggtttgc acctttttcc
     3541 tgcagcgtat tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc
     3601 tcttgttccc aaatcgcacg taatgcatcg ctgctcagga tggtggcaac aacagaagcg
     3661 ccgtgtgctg gagggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg
     3721 cgatcaacga tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac
     3781 aggccaaagt ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa
     3841 gcgcgcagtc cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca
     3901 aacagcggta accagccttt ctcaacggag agttgtgcca gtgtctgcca ttgttccagc
     3961 gtagggtcga taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc
     4021 tgagcttcgt tcagactgtt aatcagtgca tcgaagtcaa gggtgtgatt ttccgcatca
     4081 taataagcgt attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc
     4141 cagcttgggt tgctcaccca cacacgctta acgctggtat tttttgccag gaagtcggca
     4201 gccacgcgta gtgcgccagt gccacccgga gtctgtgccg tgcgagcacg tttgtcattg
     4261 atcagggcgc tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcaaggatg
     4321 ccgtcaatgc cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt
     4381 ttcacgctgg tcagtaccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg
     4441 ttaattttgc cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg
     4501 gcaggagcgg cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa
     4561 gtccgctatc agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag
     4621 gctgtttttg caagacgtga gattgctctg gaaggcataa aaaaaacagg accaacgtcc
     4681 tgttttttcg gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc
     4741 aacggtgtcg tctgaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta
     4801 gtcaacatag gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt
     4861 caccagatca acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat
     4921 ggacggacgc agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt
     4981 gttggcgaag ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc
     5041 accgtagttc gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg
     5101 ttcagctttt ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc
     5161 accataagca ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc
     5221 gtcgccgtta gaacggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa
     5281 gttcaggcca tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac
     5341 acgaccaacg aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc
     5401 ggtgtaaccc agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaac
     5461 gtcagcgtat ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc
     5521 gccttcagag ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga
     5581 attgatttga gtttcccctt taaaaccaag acgggcatag gtcatgtcgc cattgccacc
     5641 gtaactgttt tcaccgttac ccttggaaaa ataatgcaga ccaacagttt taccgtacag
     5701 atctactttg ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa
     5761 cagagcaggg acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg
     5821 tttttttatg acacctgcca ctgccgtcaa taagttctgt caataaaaat ttacggaact
     5881 attgatgaga gtttggtgtc tttatgtgtc tgcaggcatc tttccattca aactaacgtt
     5941 tcgctaccgt gaaagtgcta caaagataaa gatttagttt caaaaagaaa aaatatgtaa
     6001 ccaaaagtaa aaattaagga gctttgtgaa caccgtcata tttccatagg aacgtgatga
     6061 tatttacagc aatttttatc tatttatatg atttacttat atttaaatta actaaacgga
     6121 aattttgttt ctgatggaaa ctttatcgac ctggcacaaa atcctctttc agacatccag
     6181 aatgccaaaa aatagtatga aaattgtgct atcagctaag aaaaaactaa tccgcaataa
     6241 gagtacgggt tattttttgt gcgtaaatgt tgcaatgtaa tattccgtaa caggatgatc
     6301 gttatggcgg gggtgcaaat agattgcttg acttaacaaa cagataactt gacagaaagg
     6361 ataaaaaaga gccagcaggc gctggctctt tgaggaagaa aatcagaagc tggcgttgcg
     6421 cggagtacgt gggaacggaa tcacatcacg cacgttttgc acgccagtta cgtaagcaat
     6481 caggcgttca aaaccaagac cgaaacctga atgcggaaca gtaccgtagc gacgcagatc
     6541 gcgataccac cagtaatctt ctttattcag gcccatttcc agcatacgct cgtccagaac
     6601 gtccagacgt tcttcacgct gggagccacc aatgatctca ccgatgcccg gagccagaac
     6661 gtccatagcc gcaacggttt taccgtcttc gttgaggcgc atatagaacg ctttaatatc
     6721 tttcggatag tttttaacca ctaccggtgc tttaaagtgt tcttccgcca gataacgctc
     6781 atgctcagaa gagagatcga cgccccagta aaccgggttt tcaaacttcc tgccgcagtt
     6841 ttcgagaatg gtcactgcgt cggtgtaatc cacctgcgcg aaatcggctt caatgaagcg
     6901 ttccagacgt gaaacggcat ctttatctac gcgttcagcg aagaatttca tgtcgtctgc
     6961 gcgttcttcg agaaccgctt tgaagacata tttcagcatg gcttcagcca gacccgcgat
     7021 atcgttcagg ttagcaaacg ccacttccgg ctccagcatc cagaattccg ccaggtgacg
     7081 gctggtgttg gagttttcag cacggaaagt cgggccgaag gtataaattt tggataatgc
     7141 gcaagcgtag gtttcgccgt tcaactggcc agatacggtc aggaaagact ctttaccaaa
     7201 gaagtctttg tcgaaatcca ctttgccctg atcgttacgc ggcaggtttt ccagatccag
     7261 cgtagaaacg cggaacattt cgcctgcacc ttcggtatca gatgcggtaa tcagtggcgt
     7321 tgaaacccag aagaatccct gctcgttaaa gaagcgatgc agcgcctgcg ccagcgtatg
     7381 gcgaacgcgc gcgacggcac caatcaggtt tgtgcgcgga cgcaggtgag cgacttcacg
     7441 cagatactca atgctgtggc gttttgccgc catcgggtaa gtgtctggat cttcaaccca
     7501 accagcaact tcaaccttgc tggcctgaat ttcaaattgt tgcccctggc ccggcgatgc
     7561 cacgacttta cccgtcacaa tgaccgagca gccggtggtc agacgcagga cgtcttcatt
     7621 gtaattgggc agagaattat tgatgacagc ctgtacagga tcaaagcagg aaccgtcata
     7681 aacggcgagg aaggagatgc cagcttttga atctcggcgg gtacgtaccc atccgcgtac
     7741 ggtgacttcg ctgtcaacgg ctacgcggcc ctggagtacg tcggctacag gcacaacgct
     7801 cataatattc tctctgttaa tagtcggaaa aaataaacac ttgtccaccc gtaaatgggg
     7861 gggataccta tgttacctgg catctgcaat cagacaagca gaattcgcaa atgcagcgaa
     7921 agatttcgga attaaaggta aaaaagggag ccgattagct ccctgatgat attaactggc
     7981 ttttttaata tgcggaaggt cgaacgcttt gcgcagcgcc cgaacaaacg ctttatcatg
     8041 gcagatagtt ttgccagggc tgtcagaaag tttcgccacc ggtttaccgt tacactctac
     8101 caacttaatc acaatattca ggggttttac ctgggggata tcgcaggtca ggcgagtccc
     8161 aataccaaaa cttaattgca cgcgggaaga gaagtggcgg tatagctcaa ccgctttgcg
     8221 taaatccaga ttgtcagaga aaaccagcgt tttactctgt ggatcaattc cctgcttttc
     8281 ataatgtgca atggcttttt caccccattc aaccgggtcg ccagagtcat gacgcaggcc
     8341 ctgataccga ctagcgaact cgacaccgaa atcacgcagg aaagcatcca tagtgatgca
     8401 gtcggttaat gcaatgccaa gttggtcggg atactcttcc agccaggcag caagtgcagc
     8461 tcgctggctg ttggctagat ccgggctgat ttgctgatgt gcctggaacc attcgtgtgc
     8521 ctgtgttccc atcggcgtga gggaaagccg acgcgccaga tcgtagttgc tggtgcccac
     8581 gaaccaggat tcctgttgca gacgcttaac gatggtttct tgtacttcgc gagaaaaacg
     8641 gcgacgggtg ccaaaatcca tcagatggaa gcgcgacata tcaagaccgg cggttaacgc
     8701 cgagaagtcg actaatttgc tttccagcgt gtcgagggct tgcgcaacgt cggcctgcgg
     8761 tgagcgatag cgatgtacca tttcactgat aaccgccagc aaaggaactt cccagaggat
     8821 gacttcacgc cacggaccgc ttaagcgaat atccagcttg ccattatcgt tggacacggt
     8881 gacttgttcc gggttaaagc ggaactcgcg taaccagtta agatagtcgg ccttaaagaa
     8941 aggcagggca gagagccact gatattcatc atcctgcagg cgcaggtgct gcatcgcctg
     9001 aatctgttca cgaatagcat cggcataaat acccagcaga tcgtcacctc ggcaacgaaa
     9061 ctccgccgcg acatgcacat cgtaatagtg atgaaacacg gcttgctgca tatgcaactt
     9121 ataagcatct gtatccagca acgagtgcag aacaggagaa gcgaattgtg tcataggtgc
     9181 gcagtagcgt cctcgtacag gagcgtttag tacaataaac atcttcagga actgctggag
     9241 tataccttgt ttcgcaattt attgaacccc gatcacacca tatgccacct ttctggtcga
     9301 tggcattacg cggtgtatgt tataaaaatg tagcaataaa ggcgtttgta cctgaaaaga
     9361 tgaagattct gcatagcgcg atttacgcaa caggaataga ctgaacacca gactctataa
     9421 aagatgctaa aggttattta tgactcaaca gccacaagcc aaataccgtc acgattatcg
     9481 tgcgccggat taccagatta ctgatattga cttgaccttt gacctcgacg cgcaaaagac
     9541 ggtcgttacc gcggtcagcc aggctgtccg tcatggtgca tcagatgctc cgcttcgtct
     9601 caacggcgaa gacctcaaac tggtttctgt tcatattaat gatgagccgt ggaccgcctg
     9661 gaaagaagaa gaagagggcg cactggtcat cagtaatttg ccggagcgtt ttacgcttaa
     9721 gatcattaat gaaataagcc tggcggcgaa tacggcgctg gaagggcttt atcagtcagg
     9781 cgatgcgctt tgcacccagt gtgaagccga aggtttccgc catattacgt attatctcga
     9841 ccgcccggac gtgctggcgc gttttaccac caaaattatt gccgataaaa tcaaatatcc
     9901 cttcctgctt tccaatggta accgcgttgc gcaaggcgaa ctcgaaaacg gacgccattg
     9961 ggtacagtgg caggacccgt tcccgaaacc gtgctacctg tttgcgctgg tggcaggcga
    10021 ctttgatgta ctgcgcgata cctttaccac gcgttctggt cgcgaagtag cactggagct
    10081 gtacgtcgat cgcggcaacc ttgatcgcgc gccgtgggcg atgacctcgc tgaaaaactc
    10141 catgaaatgg gatgaagaac gctttggcct ggagtatgac ctcgacatct atatgatcgt
    10201 cgcggtggat ttcttcaata tgggcgcaat ggagaataag ggtctgaata tctttaactc
    10261 caaatatgtg ctggcccgca ccgacaccgc caccgacaaa gattacctcg atattgaacg
    10321 cgttatcggc catgaatatt tccataactg gaccggtaac cgagtgacct gccgcgactg
    10381 gttccagctc agcctgaaag aaggtttaac cgtcttccgc gatcaggaat tcagctctga
    10441 ccttggttcc cgcgcagtta accgcatcaa taatgtgcgc accatgcgcg gattgcagtt
    10501 tgcggaagac gccagcccga tggcgcaccc aatccgcccg gacatggtca ttgagatgaa
    10561 caacttctac accctgaccg tttacgagaa gggcgcggaa gtgatccgta tgatccacac
    10621 cctgctgggc gaagaaaact tccagaaagg gatgcaactc tatttcgagc gtcatgatgg
    10681 tagcgcagcg acctgtgacg actttgtgca ggcgatggaa gatgcgtcga atgttgatct
    10741 ctcccatttc cgccgttggt acagccagtc cggtacgccg gttgtgaccg tcaaagacga
    10801 ctacaatccg gaaactgagc agtacaccct gaccatcagc cagcgcacgc cagccacgcc
    10861 ggatcaggca gaaaaacagc cgctgcatat tccgtttgcc atcgaattgt atgacaacga
    10921 aggtaaagtg atcccgttgc agaaaggcgg tcatccggtg aattccgtgc tgaacgtcac
    10981 ccaggcggaa cagacctttg tttttgataa tgtctacttc cagccggtgc ctgcgctgct
    11041 gtgcgaattc tctgcgccag tgaaactgga atataagtgg agcgatcagc aactgacctt
    11101 cctgatgcgt catgcgcgta atggtttctc ccgttgggat gcggcgcaaa gtttgctggc
    11161 aacctacatc aagctgaacg tcgcccgtca tcagcaaggg cagccgctgt ctctgccggt
    11221 acatgtggct gacgctttcc gcgcggtgct gctcgatgag aagattgatc cggcgctggc
    11281 ggcagaaatc ctgacgctgc cttctgtcaa tgaaatggct gaactgttcg atatcataga
    11341 cccgattgct attgccgaag tacgcgaagc actcactcgt actctggcga ctgaactggc
    11401 ggatgagctg ctggctattt acaacgcgaa ttaccagagc gagtaccgtg ttgagcatga
    11461 agatattgca aaacgcactc tgcgtaatgc ctgcctgcgc ttccttgctt ttggtgaaac
    11521 gcatctggct gacgtgctgg tgagcaagca ataccatgaa gcaaacaata tgaccgatgc
    11581 gctggcagcg ctttctgcgg cggttgctgc acagctgcct tgccgtgacg cgctgatgca
    11641 ggagtacgac gataagtggc atcaggacgg tctggtgatg gataaatggt ttatcctgca
    11701 agccaccagc ccggcggcga atgtgctgga gacggtgcgt ggtctgttgc agcatcgctc
    11761 atttaccatg agcaacccga accgtattcg ttcgttgatt ggtgcgtttg cgggcagcaa
    11821 cccggcagcg ttccatgccg aagatggcag cggttaccag ttcctggtgg aaatgcttac
    11881 cgacctcaac agccgtaacc cgcaggtagc ttcacgtctg attgaaccgc tgattcgcct
    11941 gaaacgttat gatgccaaac gtcaggagaa aatgcgcgcg gcgctggaac agttgaaagg
    12001 gctggaaaat ctctctggcg atctttacga gaagataact aaagcactgg cttgatactt
    12061 ttgagccaaa atgcctgatg cgacgcttga cgcgtcttat caggcctaca actccgttga
    12121 aattgctggt tttgtaggct ggataaggcg ttcacgccgc atccggcagc cgtgcccaac
    12181 tgcctgatgc gatgcttgac gtgtcttatc aggcctacaa gtccgttgaa aatgctggtt
    12241 ttgtgcgcca catccggcat attaaccatg acgccgaatc agttgttcag aatgcccacg
    12301 ccgcattacc cgttgcaata cttccgcttc cagctccgct aatcgcaccg accctaatcg
    12361 acgcggacgg gggatatcca ctgtcaaatc cagactaatt tttccctctt caattaacaa
    12421 cacccggtca gccattgcta ccgcttcgct cacatcatgc gtcaccaaca gtacggtaaa
    12481 gccgtgctgc tgccaaagtg acacaatcaa atcctgcatc tcgagtcgcg ttaaggcgtc
    12541 cagtgccccg agcggttcat caagcaacaa taaacccggt cgatgaatca acgctcttgc
    12601 cagcgccaca cgctgtttct gcccgccaga aagtgcggca ggccattccc cggcgcgatt
    12661 ctccagtcct gccgcagcca acgcccgacg cgcggcatcg cgccactgac ctttaaggcc
    12721 taacccaacg ttatcaatca ccgatttcca cggcagcaga cgcgcatcct gaaacatcat
    12781 tcgcgtatca tcctgaattt cagccagcgg tgtggtgccc gctaacacat cgcctgcggt
    12841 tggcgtttcc agaccggcca gcaggtgcag cagggtactt ttaccaccac cgctgcggcc
    12901 caccaccgcc acaaactgac ctgccggaat atgtaaatcc agttggttca ggacgatatt
    12961 ttccgcgtaa tgtttgctta ctgcattgag caacaatggt gtgccctggt tcagacgagc
    13021 agtattcata tcgtggcctc cttcaaatga taagccgggt tccagcgcaa ccagaggcgc
    13081 tctaacagct gcgcgctgac gtcagcaagt ttgccgagca gggcgtaaag aataatagcg
    13141 actaccacca cgtctgtttg caaaaactct cgcgcattca tcgccagata accaatgccg
    13201 gaattggcag aaatggtttc ggcaacaatc agcgtcagcc acatcaggcc caacgcaaaa
    13261 cgcacgccga ccataattga gggcagggca tcgggcagga tcacatggat aaacagcggt
    13321 atgccggata atccatagct acgcgccatc tccaccagcc cgcgatcgat attacggatg
    13381 ccatgccagg tgttgatata aatggggaac agcgtaccga gcgccaccag aaagattttc
    13441 gcggattcat caatgccaaa ccacaaaatc accagcggga tcagcgccag atgcggcacg
    13501 ttgcgcaaca tctgaattga ggtatccagc agcctttctc cccagcgcga cagtccgcta
    13561 atcagtccca gaatcaggcc cagcgatccg ccaattgaaa agccaatcag cgcccgccag
    13621 gaactgatcg ccagat
//
LOCUS       DAJTRH010000094        13618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTRH010000094 DAJTRH010000000
VERSION     DAJTRH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO339_003128"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO339_003128"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5927661.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO339_003129"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO339_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5927662.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO339_003130"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO339_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5927663.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO339_003131"
     CDS             3807..3995
                     /locus_tag="OO339_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5927664.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO339_003132"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO339_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5927665.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO339_003133"
     CDS             4756..5115
                     /locus_tag="OO339_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5927666.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO339_003134"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO339_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO339_003135"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO339_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5927667.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO339_003136"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO339_003136"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5927668.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO339_003137"
     CDS             10913..11254
                     /locus_tag="OO339_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5927669.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO339_003138"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO339_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO339_003139"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO339_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5927670.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
BASE COUNT         3195 a         3474 c         3716 g         3233 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaag
//
LOCUS       DAJTRH010000095        13491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTRH010000095 DAJTRH010000000
VERSION     DAJTRH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..1285)
                     /gene="aldB"
                     /locus_tag="OO339_003140"
     CDS             complement(<1..1285)
                     /gene="aldB"
                     /locus_tag="OO339_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5927671.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALEL"
     gene            complement(1450..2601)
                     /gene="yiaY"
                     /locus_tag="OO339_003141"
     CDS             complement(1450..2601)
                     /gene="yiaY"
                     /locus_tag="OO339_003141"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5927672.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(2791..4635)
                     /gene="selB"
                     /locus_tag="OO339_003142"
     CDS             complement(2791..4635)
                     /gene="selB"
                     /locus_tag="OO339_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5927673.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(4632..6023)
                     /gene="selA"
                     /locus_tag="OO339_003143"
     CDS             complement(4632..6023)
                     /gene="selA"
                     /locus_tag="OO339_003143"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5927674.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(6121..6729)
                     /locus_tag="OO339_003144"
     CDS             complement(6121..6729)
                     /locus_tag="OO339_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5927675.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(6800..7885)
                     /locus_tag="OO339_003145"
     CDS             complement(6800..7885)
                     /locus_tag="OO339_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5927676.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(7888..8250)
                     /locus_tag="OO339_003146"
     CDS             complement(7888..8250)
                     /locus_tag="OO339_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5927677.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            8787..10700
                     /gene="mtlA"
                     /locus_tag="OO339_003147"
     CDS             8787..10700
                     /gene="mtlA"
                     /locus_tag="OO339_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5927678.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            10795..11943
                     /gene="mtlD"
                     /locus_tag="OO339_003148"
     CDS             10795..11943
                     /gene="mtlD"
                     /locus_tag="OO339_003148"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5927679.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            11943..12530
                     /gene="mtlR"
                     /locus_tag="OO339_003149"
     CDS             11943..12530
                     /gene="mtlR"
                     /locus_tag="OO339_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5927680.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(12542..12751)
                     /locus_tag="OO339_003150"
     CDS             complement(12542..12751)
                     /locus_tag="OO339_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927681.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13035..13397
                     /locus_tag="OO339_003151"
     CDS             13035..13397
                     /locus_tag="OO339_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5927682.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3259 a         3621 c         3426 g         3185 t
ORIGIN      
        1 ccagctccag cgcttcttcc atcgttttga aggtggtcac cgccagcacc gggccaaaaa
       61 tctcctcctg gaacacccgc atattgttct gaccaaacag aatcgtcggt tcgaggtagt
      121 agccgtcttt cagttcacct tccagcagct tgcgccgccc gcctgtgagc acgtcagcgc
      181 cctctttttt accgatatca atgtagttga ggatggtttc cagttgcccg tgagaaacct
      241 gcgcgcccat ttgcgtcacg ctgtcgagcg ggttaccgct gcgaatgctt tcgacacggc
      301 ggatggcgcg ttccataaag cgttcgtaga ttgactcctg cactaaggca cgactcggac
      361 aggtgcaaac ttcgccctgg ttaaaggcaa acagtgcaaa gccttccagc gctttatcga
      421 aaaaggcatc ttcttcatcc atcacatcag caaagaagat atttggcgac ttaccgccca
      481 actccagcgt caccggaata atgttttgcg ttgcgtattg cataatttgt tggcccactt
      541 ccgttgagcc ggtaaacgcc actttggcga tgcgtttcga ggtcgccaga tattcgccaa
      601 tttccccacc tgcgccattg accacgttca ccacgcccgg cggcagtaaa tcaccgacaa
      661 tttccattag cagcagtaca gaaagcgggg taagacgtgc gggtttcagc accacacagt
      721 tgcccgccgc cagcgcggga gccattttcc agctcgccat cagcagcggg aagttccacg
      781 ggataatctg ccccaccacg cctaacggtt catggaaatg ataggccacg gtttcgctat
      841 caacttcact gatcccacct tcctgcgccc gaatacacga ggcgaaatag cggaaatggt
      901 caatcgccag cggtacatcc gcagcactgg tttcgcgaat gggtttgccg ttatcccagg
      961 tttcagctgt cgctaacagc tcgaggtttt gttccattcg atcggcaatc ttaaacagaa
     1021 tcgccgcgcg atcctgcacc gaggtgtgcg cccatttatc tttcactttg tgcgcagcat
     1081 ccagcgccag atcgatgtct cgtttgcccg aagacgccac ttcgcacagc agctgcccgg
     1141 tcaccggcgt cagattctgg taatactcac catcggcagg ggctacccat tcaccgccaa
     1201 taaagttgtc gtagcgggct tttaacttga tggggaaacc atactcgccg ggcttaatct
     1261 gtgctgaagg gggattattg gtcatgatcg tctccttgcg gtgtgaggta taacaagggt
     1321 agacgtgact ggcgaaatct tcgccagtcg gtaacagctt tacgacagct atcacgaatt
     1381 tacgggcaag gattacagag atagttgaaa aagcggctga caatttgcca gccgttgttg
     1441 gaatgatgat tacattgcgg cgcgataaat caccacaatt tcttcgtgag ttgcctggat
     1501 cgggttagta aagccacagg catctttcag ggcattagtc gccagaaccg cgaaatcttc
     1561 ttctttcacg ttcaggtcgc gtaggcctgc cgggatatcc actttcttcg ccagttcacg
     1621 gatggcgtta atgcaggctt cagcaccttc cgcgtcgttt ttacctgtca cgttcacgcc
     1681 cattgcagcg gcacagtcac gcagacgtgc agcggcgact ttgctgttga atacctgaac
     1741 atgcggcagc agaacggcgt tacatacacc gtgtggcagg ttgtagaaac cgcccagctg
     1801 gtgcgccatc gcatgaacat aacccagaga agcattattg aacgccatac cggcaaggaa
     1861 ctgggcataa gccattgctt cacgcgcttt cgcattactg ccatcttcaa cggctaacgg
     1921 caggttttcg gcaatcatgg tcacggcttt cagtgcacaa gcgtcagtga tcggcgtggc
     1981 ggcaatagaa acatacgctt cgatagcgtg cgttaaggca tccatacccg ttgcggcggt
     2041 cagtgacttc ggcataccaa tcatcagaga ggagtcattg acagaaagca gcggagtcac
     2101 gtgcttatca acaatcgcca ttttgttatg acgcgcttcg tcagtgatga tgcagaaacg
     2161 ggtcatttca gaggccgtac ccgccgtggt attgatggcg atcatcggca gctgcggttt
     2221 tgcagagcgg tcaacgcctt cgtaatcgcg aatatcgccg ccattggctg ccaccagcgc
     2281 aatacctttt gcgcagtcgt gtggagaacc accgcctaag gagatcacgc tatcgcaatt
     2341 attttcttta agtaatttca aacctgcggc gacgttttcc gtggtggggt taggttgggt
     2401 gccatcataa ataacgctaa aaatattgcg ttcttccagt gctttttgca catcacccgc
     2461 catacctaat ttcgttaaca tattgtcagt gacaattaag gtacgggtaa atccataatc
     2521 tgccatcata ttcattgcat cagtcaatga atcagcgccg atgacattca cagaaggaat
     2581 aaagaaagtt gaagatgcca taacacactc ctgaaagtgt aaaaaagggt gggcagctta
     2641 ctgaggattt tcattcgaaa acatgatcat tattattaat tatcaaaatt aatctaataa
     2701 aaagcacatt gtattcatta aacaatgtgc tttatttaaa atcataagaa atttgtatta
     2761 atttatttta aaaattaatt aatcatttcc ttatttttcc ggaaataata atgcgtcgcg
     2821 caataaatga tcatttccac gacgacgcgt aaagccaatg cggtcaaaat attccagaat
     2881 ctgaattgcc agctttcggc ctacgcctaa gcgatcgcgg aaatccgccg cgcaggttga
     2941 accacactcc tgatcgagat cgcggatcat attggcaaat tcgacaatcc gatcgttacg
     3001 gtaataacga tctttgacga tcgcggtaat tattccttgc tgcgccgcct ggcgtagcgt
     3061 caggcgcatt gcctgctcgt cggtacccgt ctcttttgcc agatcacgca cccaccacgg
     3121 ctcgtcacca aacagcggct ctgctttttg ccagatggcc tgctgctctt cgctgaagcc
     3181 cgctttgtga tccggcagat gcagccaacc gtgatgactg aggatgtcgc cgctttcgcg
     3241 catcttttca atcagcaaca gtactagcgc ttcatcttcc attggcaacg ccatacgtcg
     3301 cagacgttcg cgcccaggcc caggttcatc gcgatgttgc tcatgataag tcgctaatgt
     3361 gtcgagaatt ttccgttgcc agcgagcggc aaccggcgca ttcaacaagc tataaccagc
     3421 ctgaatatag ccaggctgtt gcagcagttc gcgcatccct tcgccgttga gctggcgcgc
     3481 ccaagcgaaa tcaggaagat taaccgcgcc gcgttgcagg tgaacggcta acgcttcgct
     3541 atcattctgt gccctggcga gggaagccag ccattgcaga tactctggtt tacgtttacc
     3601 gcgacgcggc gggttaagca tcacgacgcg cgctccggcc agcgtgttgc gggcagagat
     3661 atcgcgcaat accaggcggt cgttatctgc cagccataac ggggtgtcga agaccagttc
     3721 agcaaggtta tcttccagca gtgaaacgcg tcccgtgacg tggctggcgg cgtggtgaat
     3781 atgcagcggc tgccactggg tcagcggtgc atgggtttga agctcgacaa tcacccgtgt
     3841 gaacggctct ggcggcacat cggcaagcag ccagtcgcca cggttaatct gctctttttc
     3901 cgcatcaccc gcgatgtcaa gcgcgatacg ctgcccggca ttggcggttt ctgttggctg
     3961 gttttgcgca tgcagcgcac gtacacgcat cggtttattt acaccagtca gccagagtga
     4021 atcgcctacc ttcacttccc cgcttaacgc cgtaccggtg acgaccagcc cggcaccttt
     4081 tacggtaaat gcgcggtcaa tcgcgaggcg gaaactatgt tggctggcgt gctcgcgttc
     4141 cggcaactga agcagatgct cgcgcagggc atcaattccc cgaccttcgg ttgctgcggt
     4201 gataaacagt tttgcctcag caaaaccgta ttcccgcaga acctccttta cctggcgttc
     4261 aacctcatca acacgcgctt cgtccacgcg atcggctttg gtcagcgcca ctgtcagcat
     4321 cgggttaccg gtcagctgca aaatcgccag atgctcacgg gtctgtgcca tcacgccgtc
     4381 atcgcacgcc accaccaaca gcgcgtgatc gataccacca acgcccgcca gcatgttgga
     4441 aagaaacttt tcatgaccgg gaacgtcgat aaaaccaggc acgcgaccat ccggctgcgg
     4501 ccagtaggca tagccgagat cgatggtcat gccgcgcttt ttttcttccg gcagacggtc
     4561 agcatttacg ccagtaatcg cctgcaataa ggttgttttg ccgtggtcaa cgtgtccggc
     4621 agtcgcaata atcatttcaa caacatctcc aaaaaccgtt gctcatcttc aaggcagcgt
     4681 aaatccagcc acaatcgtcc gtcataaata cgaccaatca ccggcactgg caattcacgc
     4741 cagcgggcgg ctaatgactc aaggtggcta ccgcgtccat catggggtgt aaacgttaat
     4801 gccgcgctcg gcaggcgatc aaccggcagc gaaccactgc caatctgcga aagacatggc
     4861 ataacctgta ccgcaaactc cgcgccgtaa tgtgcgacaa ggggagcctg taaacgttgt
     4921 gcctggattt gaatgacctc tgcgctgcgg gtaagcaggc gcagggtcgg taatttttca
     4981 ctcagagctt cagggtgtaa ataaagacgc aacgtggctt ccagcgccgc gagggtcatt
     5041 ttatccgcgc gtaatgcacg cttcagcggg tggctttgca ggcgggcgat catctctttt
     5101 ttaccaacga taatcccggc ctgcggcccg cctaacaact tgtcgccgga gaagctcacc
     5161 agactgacgc ccgccgcaat caactcctgc ggcattggct ctttcggcaa accgtactgg
     5221 ctaagatcga ccagcgagcc actgcctaaa tcagtcacca ctggaacatc cagctctttg
     5281 ccgagcgcca ccagttcagc ttcgtctatc gctttggtga acccctgaat gctgtagtta
     5341 ctggtatgta ctttcatcaa cagtgcggta ttttcattca ccgcctgacg ataatcattc
     5401 gcgtgcgtgc ggttggtggt ccctacttcg tgtagggtgc agcctgcctg acgcataaca
     5461 tcgggaatac gaaacgcgcc gccaatctcc accagttcgc cgcgagatac caccacctct
     5521 ttcccgctgg cagtggccgc caacatcaat aacaccgccg ccgcattgtt attgacgata
     5581 caggcatctt ccgcccccgt aatacggcac agcagctgcg ccagcgcccg atcgcgatgt
     5641 ccgcgtccgg cgtcgtccag atcgtactcg agggtcactg gcgaacgcat agcctgcgca
     5701 acggcttcca ccgcggcttc cgcctgtaaa gctcgcccaa ggttggtatg cagcacggtt
     5761 cccgtcaggt tgatcaccgg acgcagcgcg ctctgcgcct ctttcgtcaa ccgggcatcg
     5821 acttcttgcg cccagttttc acaccacgca ggcagcgtct ggctgccacg aatcacttct
     5881 cgcgcttcgt cgagcatctg acgcaacaat tccaccacgc gggtgtgacc ataagtatca
     5941 cgcaaagaaa ggaaggagct atcgcgcaat aagcgatcaa tagccggaag ttgactatag
     6001 agggaacgcg tttcggttgt cataggaaac ctggctgatc aaggccctct cacacggaga
     6061 agggcgttta acataaccac ggattgtaac gtgagatggg tcaggaggac atatcgcgca
     6121 tcaagccttt ggcggttcgg tgcgagcaaa actttcgcgg ctaaacaggt tttcgaccag
     6181 tttgactaag tgtgggcggt cgacacacca acctggcgca acgcggcgga aattgagata
     6241 tccgacagca caggcaatgg cgatagtcgc cagattgacc gtatcggttt tgagtgtgcc
     6301 atcgaccaga tatccttcca gcacatccag actgcggttg attttctccc gctggcgtaa
     6361 caattcatct tcagactgct gcgccgccgg acgcgcctgt tcacgcaccg ataccagccc
     6421 ggcatccata atgccatccg ccagcgcctc aattttgcgc acccgcaacg actccagcgg
     6481 atcgcgcggc aacatcgccg gagcgacatt cattaactca atatattcag cgatgatcgg
     6541 cgaatcaaac cagcattcgc cctcttcggt caccagcacc ggcactttcc ctaacgggtt
     6601 aaattgcgcc acgccgttat ccgcgttata gggcagttca ttaataaatt cgaaagttat
     6661 gcccttttct aacaacagaa taaaaagttt gcgtacaaac gggctggtgt agctaccgac
     6721 gagtttcatg ccgagtcctt tgtgcgagga aaaatatcag tatggctcag gctcacgcaa
     6781 aacacaggtg atataagtct caatgatcca aataaagata atgcatccaa ctggtcattc
     6841 ttagcagcac tttccgcatc accgaaacat ggctgaaatg gtcggagtaa accgccacct
     6901 gggcgtagat gccgtcgggt aaggcatcga tatcatcgtt agggtccagt tcaatggttc
     6961 ccagcacacc gtccgtgccg ggaacgaccg ttaatgattg caataccccc tgcgcctgat
     7021 aagaaccgcc tggcacgaca ggtaaaatac tagtcaattt gccgtggaac acctgcccag
     7081 gtagcgcgtt aaacaccact tccgcatcat cgccaggttt cagacgtaac agcgagtttt
     7141 gccgaaattg ggcgacaatt tgccgttttt gctcggggat gaagaccatc accggacgca
     7201 gcggcaaggc agctgcgtat gtacctgggc ggatcagtac ctgagtgacg tagccattgc
     7261 ttggtgcgcg aatgacagtc tgctcaagat tatattttgc ttcagtaagt tgcgctctta
     7321 agctcacaat ctgcgattgc tcgccgttga ccatactgtc gagctgactc tggatctgcg
     7381 cctgctccgc caccgagcct ttcactaacg catcctgcgc gaggaaattt tgccgcgcat
     7441 cgtcgatgtc acgttccgag aacggattca ccgccgcctg gctgcctttc aggtaacgtt
     7501 gataattttt aaacagacgg tcgcgctccg ctgaaacctg ggtggtgttg gcctgcgctt
     7561 cagtgagctg cgcacgcagc gtctttatat tatgcgtcgc cgtcatcagg tcagcctgaa
     7621 gtctgtcaac tcgcgcctgg taacgaaccg ggtcgagctt aaaaagcacc tcgccctttt
     7681 gaataagctg attattcttg tcagtgactt cagtaacaat tcccgtcacc tgtggcgtga
     7741 tagggatcgc tatcactgcc ttttgcgcgg taaaagtgta agggtggttg tagttcatca
     7801 acaaaatcaa accactcacc agaaacacgc ctcccagcgc cgccgtcgcc agcgccacgt
     7861 aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt
     7921 tttctcggcg gcgagttgat gctccagccg ggaaattcgg tcagaaagtg cggcgatttc
     7981 cggttcagtc gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg
     8041 ttgatagagc gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct
     8101 cacccagccc gccgtatgaa tagcgtcggc atgggggtga ttgcgctttt tggcaatcag
     8161 ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag
     8221 cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt
     8281 gttttttggc tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg
     8341 cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct tagttacata
     8401 acgattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa
     8461 atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacatta
     8521 ctcacattta aatgccattt ttagcgaaaa tcaccgcctt gtttcttttt tatacaggcg
     8581 ttttgtgatg agcgtcacgt caattacctc tctaccccct atatttatgt gactgatatc
     8641 acacaaaagc ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac
     8701 aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga
     8761 cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct
     8821 ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta
     8881 tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg
     8941 tcgggccgat gatcacttat ctcctaccgc tgctgatcgg ttataccggt ggtaagctgg
     9001 taggcggcaa acgtggtggc gtagtcggtg ccattaccac catgggcgtt atcgtcggcg
     9061 cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta
     9121 agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata
     9181 acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc
     9241 cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg
     9301 acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg
     9361 ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat
     9421 caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca
     9481 tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc
     9541 tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg
     9601 cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg
     9661 tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt
     9721 atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta
     9781 ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta
     9841 tgcaggacat gaaagctgag tctaaaggca catctccgct atctgcgggc gatgtgacta
     9901 acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttcaagtg
     9961 cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtcg cagatttcgg
    10021 tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg
    10081 acctgaccga acgcgctatg cgccaggttc cgcaggcaca gcatatttcg ctgaccaact
    10141 tcctcgacag cggcctgtac accagcctaa ccgaacgtct cgttgctgcc cagcgccata
    10201 ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat tccagtgcta
    10261 acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca accaaagaag
    10321 aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggaatacg
    10381 ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg
    10441 tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg gtgttctgcc
    10501 agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt ctggtgattg
    10561 gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc aatgcactgg
    10621 atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac
    10681 tgctggcagg tcgtaagtaa tccaatccta ccctctcctc acggagaggg tttgggtgag
    10741 ggaaaagcct caccccagcc ctctcgggta aaaacattga tgaaggttaa tactatgaaa
    10801 gcattacatt ttggcgcagg taatatcggt cgtggcttta tcggtaaact gctggcagac
    10861 gcgggtatcc aactgacgtt tgccgatgtc aatcaggtgg tacttgatgc cctgaatgcc
    10921 cgtcatagct atcaggttca tgtggtcggt gaaaccgagc aggtagatac cgtttccggc
    10981 gtcaatgctg tcagcagcat tggtgatgat gtcgttgatc tgattgctca ggtcgattta
    11041 gtcactaccg ccgttggccc ggttgtgctg gaacgtattg ctccggctat cgccaaaggg
    11101 ctggtgaaac gcaaagaaca aggtaatgaa tccccgctga acatcatcgc ctgtgaaaac
    11161 atggtacgcg gcaccacgca gctgaaaggt catgtgatga acgccctgcc ggaagacgcc
    11221 aaagcgtggg tagaagagca cgttggcttt gtcgattccg ccgttgaccg catcgtaccg
    11281 ccttcggctt cggcaactaa cgatccgctg gaagtgacgg tagaaacttt cagcgaatgg
    11341 attgtcgata aaacgcagtt caaaggcgca ctgccgaaca tcccaggcat ggagttaacc
    11401 gacaacctga tggcatttgt cgaacgtaaa ctcttcaccc tgaacacggg tcatgctata
    11461 accgcgtacc tcggaaaact ggccggtcat cagaccattc gtgacgctat tctcgacgag
    11521 aaaatccgcg cggtggtaaa aggtgcgatg gaagaaagtg gtgcggtact gatcaagcgc
    11581 tacggctttg acgcagacaa acatgcggcg tacatccaga aaatcctcgg tcgttttgag
    11641 aacccgtatc tgaaagatga tgtagagcgc gtaggccgtc agccgctgcg taaactgagt
    11701 gctggcgacc gtctgatcaa gccactgctc ggtacgctgg aatacagcct tccgcacaag
    11761 aatctgattc aggggattgc tggtgcaatg cacttccgca gtgaagatga tccgcaggct
    11821 caggaactgg cagcactgat cgctgacaaa ggtccgcagg cggcgctggc acagatttcc
    11881 ggtcttgatg ccaacagcga ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa
    11941 taatggtgga ccaggcgcag gacaccctgc gcccgaataa cagattgtca gatatgcagg
    12001 caacaatgga acaaacccag gccgttgaaa accgtgtgct tgagcgtctg aatgctggca
    12061 aaaccgtgcg aagctttctg atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc
    12121 tggtgcttca ggtattccgc aaagacgatt acgcggtgaa gtatgctgta gaaccgttac
    12181 tcgacggcga cggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg
    12241 gcgtcattaa ccgccaggaa tacgaagatg cggaactgct gatggcattg cgtgaagagc
    12301 taaatcacga cggcaacgag tacgccttta ccgacgacga aatccttgga ccctttggtg
    12361 aactgcactg cgtggcggcg ttaccaccgc cgccacagtt tgaaccagca gactccagtt
    12421 tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg atcaacaatg gtcctttcac
    12481 tgactgagct gatttccaaa atcagcttaa aaaaagcctt tcaaaagtaa gcaacgtctg
    12541 cttactgccc ctctacctgc ttcggccgat aaagccgacg ataatactcc agacgttgta
    12601 gatataacgg cacgctttca tcgggtattc cggacggaat cgcgttacgg ggaggaagtt
    12661 ttttcagata ctcccggaac gcctggcttg atgccatgaa atctacggct ttatcgataa
    12721 gaagcggaac gttttcacct aatttgccca tgatgatatc tccgtattac cccgcgccgg
    12781 gaatgcgcgg ccgccaattt tagtttaggt tcccggtaaa gatgacatta aggaaaacgt
    12841 gctgaatcct caataatcat ccgctgctgt ttattatctt tttctataga tttcttaatt
    12901 aatcaacgaa ttgcgtagtt tttgagcatg ttaatgatta cgttgcagaa ctattacaat
    12961 gtcatcagtt gtgaacaaag cacctggtcg cgcatactag gggctataaa tttatcttta
    13021 tcagaagcca tcacatgaaa gaagtcgaaa aaaacgaaat caagcgtctc agcgatcgcc
    13081 tggacgccat ccgccaccag caggccgatc tgtcgctggt tgaagccgca gacaaatatg
    13141 ccgagctgga aaaagagaaa gccacgctgg aagcagaaat tgctcgcctg cgtgaggttc
    13201 atagccaaaa actgagtaaa gaagcacaga agctgatgaa gatgccgttc cagcgcgcaa
    13261 ttaccaaaaa agagcaggct gatatgggca agctgaagaa aagtgttcgc ggactggtgg
    13321 ttgtgcaccc aatgaccgcg ctggggcgcg aaatgggcct ggaagagatg actggatttt
    13381 ctaagactac gttttaagcc cctcagacga ctggttcgat cgtatcaacc cgcatctggc
    13441 ttgccttagc tggtcaatgc ggggactctc ctgtctactc gcctaattac c
//
LOCUS       DAJTRH010000096        13250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTRH010000096 DAJTRH010000000
VERSION     DAJTRH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(17..999)
                     /gene="kdgT"
                     /locus_tag="OO339_003152"
                     /pseudo
     CDS             complement(17..999)
                     /gene="kdgT"
                     /locus_tag="OO339_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO339_003153"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO339_003153"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5927683.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="OO339_003154"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="OO339_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO339_003155"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO339_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5927684.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO339_003156"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO339_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5927685.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="OO339_003157"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="OO339_003157"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5927686.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="OO339_003158"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="OO339_003158"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5927687.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="OO339_003159"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="OO339_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="OO339_003160"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="OO339_003160"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5927688.1"
                     /translation="MIRKTFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9657..10103
                     /locus_tag="OO339_003161"
     CDS             9657..10103
                     /locus_tag="OO339_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5927689.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10114..11565
                     /locus_tag="OO339_003162"
     CDS             10114..11565
                     /locus_tag="OO339_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5927690.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11555..12625
                     /locus_tag="OO339_003163"
     CDS             11555..12625
                     /locus_tag="OO339_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5927691.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12625..>13250
                     /locus_tag="OO339_003164"
     CDS             12625..>13250
                     /locus_tag="OO339_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5927692.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3160 a         3458 c         3491 g         3141 t
ORIGIN      
        1 gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt
       61 tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg
      121 ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg
      181 caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag
      241 tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa
      301 ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa
      361 tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa
      421 attctcgtaa ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg
      481 cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg
      541 tcatgagcgg cccggactcc aacgacatca acacaaatgc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataggacca ttgtttttat tttggaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacatca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaaacctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagcg tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaattgatt aaaatatatt ttatggagtc cgattaatgg
     9661 cagctcttac tgcaagctgt attgacctga atattcaggg caatggcgct tattccgttc
     9721 tgaagcagtt ggcgacaata gcgttacaaa acggttttat caccgactca caccagttcc
     9781 tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg atttggttcc ggtgtcgcag
     9841 tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt attattcgcc cgcaaagcgc
     9901 aggctattga ctggaaagcc agcgatggcg aagacgtcaa ttgctggatc tgcctcggcg
     9961 tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg cacactgtgt cgcaaaatta
    10021 ttcaccagga atttattcat caactgcaac agggcgatac cgaccaggtg ctggccttgt
    10081 taaatcaaac cctcagctca taaggaagtg gcgatggagt catccttacg tattgtcgcg
    10141 atcaccaact gccccgccgg gatcgctcac acctacatgg tggcggaagc cctggaacag
    10201 aaagcccgtt ctctcggcca taccataaaa gtggaaactc aagggtccag tggcgttgaa
    10261 aaccgcttat ccagcgaaga gattgccgct gccgattacg tcattcttgc taccgggcgt
    10321 ggcctgagcg gtgatgatcg cgcgcggttt gccgggaaga aagtttatga gattgccatc
    10381 tccctggcgt tgaaaaatat cgaccagatt ttcagcgaat taccgacaaa ctcgcagctt
    10441 tttgccgcag atagcggcgt gaagcttggc aaacaggaag tgcaaagcgg cagcgtgatg
    10501 agccacctga tggcgggcgt ctctgcggcg ctgccgtttg tcatcggcgg tggtatcctg
    10561 gtggcgcttg ccaacatgct ggtgcagttc ggtttgccgt ataccgatat gtcgaaaggc
    10621 gcgccgtcgt ttacctgggt agttgaatcc atcggttacc ttggcttcac ctttatgatc
    10681 cccatcatgg gcgcttatat tgcctcgtcg attgccgata aaccggcttt cgctccggcg
    10741 tttctggttt gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga
    10801 ttcctcggcg cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgcaaa
    10861 gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa tgctgatccc gttcgttact
    10921 ctgctggttt tcggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg
    10981 ggcgggctgc tccacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt
    11041 ctggtcggtg cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc
    11101 ttctgcttct cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc
    11161 gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca ttgcgcccaa actgtttacc
    11221 cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg gtgccacagt cgcaaccgaa
    11281 ccggctatac cttacgctct ggcagctccg ttgccgatga ttaccgccaa tacgctggcg
    11341 ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca aacgtctggc acctggtttg
    11401 ggtatctttg acccgctgat cggcctgatg tcgccggtag gttcgtttta tctggtgctg
    11461 gcgattggcc tcgcgctgaa catttcattc attatcgtcc tgaaaggatt gtggttacgc
    11521 cgcaaagcga aagccgcgca acaggagctt gtccatgaac attgagttac tgcaacagtt
    11581 gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt cgcgacatcc tgataaacac
    11641 gctggaacct tgcgttaatg aaatcacctt tgatggtctg ggcagctttg ttgcccgtaa
    11701 gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg gatgaagtcg gctttatggt
    11761 cacccacatc gacgagagcg gttttctgcg tttcaccacc attggtggct ggtggaatca
    11821 gtcgatgctc aaccatcggg taaccatacg cacacacaag ggagtgaaaa tccctggtgt
    11881 gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa aggcaacagc cgctgtcatt
    11941 tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat gaggtggaaa aacgcggtgt
    12001 ggcgatgggc gattttatta gcccggaagc caattttgcc tgctggggcg aagataaagt
    12061 ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg atggctgaac tattgcagac
    12121 ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc gtggaagaag aagttgggct
    12181 acgcggggca caaacctcgg cggaacacat taaaccggat gtagtgatcg tgctggatac
    12241 cgctgtcgca ggcgatgttc cgggcattga taacattaaa tacccgctga aactgggcca
    12301 ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc aaccagaaac tggtagcagc
    12361 gttaaaaaac tgtgccgcac ataacgattt accgctgcaa ttttccacca tgaaaaccgg
    12421 agcgacggat ggcgggcgct acaacgtgat gggcggcggg cgtccggttg ttgcgctgtg
    12481 cctgccaacc cgctatctgc acgctaacag cggcatgatt tcaaaagccg attacgatgc
    12541 tctgctcacg ttgatacggg gttttctgac gaccttaacc gcggagaaag tcaacgcgtt
    12601 tagccagttc cgtcaggtgg attaatgcta aacgaacgcc agttaaagat tgtcgatctg
    12661 ctggagcaac agccgcgcac gcctggtgag ctggcgcaac agactggcgt ttcaggcagg
    12721 accatcctgc gtgatattga ctatctcaac tttaccctta acggcaaagc ccgcattttc
    12781 gccagtggca gtgcgggcta tcagctggaa atcttcgagc gccgcagctt ttttcagttg
    12841 ctgcaaaagc acgataacga cgatcggctg ctggcgctgt tattactgaa tactttcact
    12901 ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa cgtgggtagc agagcgtctg
    12961 ccccagttaa aacagcgtta tgaacgcact tgttgcctgg ccagccgccc tggtttgggc
    13021 catttcattg atgagacaga agagaaactc gttatcttgc tggcgaactt gctgcgcaaa
    13081 gatccgtttt taattccgct ggcgggcata acacgagaca accttcagca tttatccacg
    13141 gcctgcgaca accaacaccg ctggccgctc atacagggtg attatctctc cagcctgatt
    13201 ctggcgattt acgccctgcg taatcaactg accgatgagt ggccgcaata
//
LOCUS       DAJTRH010000097        13121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTRH010000097 DAJTRH010000000
VERSION     DAJTRH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..880)
                     /locus_tag="OO339_003165"
     CDS             complement(<1..880)
                     /locus_tag="OO339_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5927693.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHV"
     gene            1121..1921
                     /locus_tag="OO339_003166"
     CDS             1121..1921
                     /locus_tag="OO339_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5927694.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1999..2769
                     /locus_tag="OO339_003167"
     CDS             1999..2769
                     /locus_tag="OO339_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5927695.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2817..4163)
                     /gene="mltD"
                     /locus_tag="OO339_003168"
     CDS             complement(2817..4163)
                     /gene="mltD"
                     /locus_tag="OO339_003168"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5927696.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4235..4990)
                     /gene="gloB"
                     /locus_tag="OO339_003169"
     CDS             complement(4235..4990)
                     /gene="gloB"
                     /locus_tag="OO339_003169"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5927697.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5024..5746
                     /locus_tag="OO339_003170"
     CDS             5024..5746
                     /locus_tag="OO339_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5927698.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5743..6210)
                     /gene="rnhA"
                     /locus_tag="OO339_003171"
     CDS             complement(5743..6210)
                     /gene="rnhA"
                     /locus_tag="OO339_003171"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5927699.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6275..7006
                     /gene="dnaQ"
                     /locus_tag="OO339_003172"
     CDS             6275..7006
                     /gene="dnaQ"
                     /locus_tag="OO339_003172"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5927700.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7139..7215
                     /locus_tag="OO339_003173"
     tRNA            7139..7215
                     /locus_tag="OO339_003173"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7173..7175,aa:Asp,seq:gtc)
     gene            complement(7346..8392)
                     /locus_tag="OO339_003174"
     CDS             complement(7346..8392)
                     /locus_tag="OO339_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5927701.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8406..8965)
                     /gene="tssG"
                     /locus_tag="OO339_003175"
                     /pseudo
     CDS             complement(8406..8965)
                     /gene="tssG"
                     /locus_tag="OO339_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8962..10845)
                     /gene="tssF"
                     /locus_tag="OO339_003176"
     CDS             complement(8962..10845)
                     /gene="tssF"
                     /locus_tag="OO339_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5927702.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10861..11355)
                     /gene="tssE"
                     /locus_tag="OO339_003177"
     CDS             complement(10861..11355)
                     /gene="tssE"
                     /locus_tag="OO339_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5927703.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11352..12182)
                     /locus_tag="OO339_003178"
                     /pseudo
     CDS             complement(11352..12182)
                     /locus_tag="OO339_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12169..13083)
                     /gene="tagK"
                     /locus_tag="OO339_003179"
                     /pseudo
     CDS             complement(12169..13083)
                     /gene="tagK"
                     /locus_tag="OO339_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3016 a         3404 c         3486 g         3215 t
ORIGIN      
        1 ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc gcacggtcgc
       61 tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc attaactcca
      121 ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac gcaatcccgt
      181 tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa ccgtacttca
      241 cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa gcgggttcag
      301 tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc ccgtagcggg
      361 aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc ctggcacgta
      421 agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt tcgatcaaat
      481 taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt tcacggaaag
      541 gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg atccgtaaca
      601 gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc atctcctgca
      661 aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta agttgccgcg
      721 tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc gcccggctta
      781 ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg ctttcgacga
      841 ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct gttgcaaaat
      901 agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat atgtcatccg
      961 atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc ttacataaca
     1021 atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga accctcccga
     1081 gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa acacctatgc
     1141 catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt cttttgcgag
     1201 catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta ttcggatcct
     1261 ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa agaactacgg
     1321 caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt tagtacagtt
     1381 tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc cacaacatcc
     1441 ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt tacgtgaacg
     1501 agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat tacctgacac
     1561 ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg tggatgtcta
     1621 tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc cggtcattat
     1681 ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc gctttgcgcg
     1741 ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg cgtttggccg
     1801 cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg tattggttac
     1861 gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc ctgataaata
     1921 aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt tatactcaaa
     1981 caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga aaagcagttt
     2041 agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg agatttacag
     2101 cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat gggctgcgga
     2161 gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc gtatgactta
     2221 tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact gaaaaatatc
     2281 accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt tgatattgtt
     2341 atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg tgaagtgaac
     2401 cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc gggtcatcca
     2461 gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca cgtacgaaac
     2521 tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt tgataattta
     2581 attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg tacgccagaa
     2641 gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt gagaacgtat
     2701 tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga agcacataaa
     2761 gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct ggtttgtcag
     2821 gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg ttgcagattc
     2881 gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg tttagcaatg
     2941 cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt gtttgccagt
     3001 cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg cagtttgttc
     3061 cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga aagcgtgtcg
     3121 ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac cagcgtcgac
     3181 tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc atgcttcttt
     3241 ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac gccagcgttg
     3301 aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc catttcaacc
     3361 gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg cagacgtacg
     3421 ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg cacgtactgc
     3481 ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg cgctttgttc
     3541 gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac ggtcagaagc
     3601 cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc agttgttgaa
     3661 gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata attgcgcccc
     3721 gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc gtgaggatca
     3781 aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg ttttttaact
     3841 tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg gagatagctc
     3901 ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg aattcccatc
     3961 tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga cgtcccatcg
     4021 tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc tgcagaaagg
     4081 ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag gacagaggcg
     4141 agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac gaccgatcat
     4201 aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc tttgacctta
     4261 accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt ccattaatta
     4321 aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc agaattacgg
     4381 gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa tcatttatgg
     4441 acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta tattcatgag
     4501 cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat tgatacattt
     4561 gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg tcgccgcaaa
     4621 atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt gtggtaatta
     4681 cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc tgtgttgttc
     4741 ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt tccaccagtt
     4801 cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc tccggttgcc
     4861 agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg acaatcaggc
     4921 agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg ggaatactgt
     4981 taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac cggcaagagt
     5041 ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag agctttatcg
     5101 caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt ttcatctgct
     5161 taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt ctcatcaagt
     5221 gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc atcttccttt
     5281 tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt gcaccgatcc
     5341 gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc tggtgattag
     5401 tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat tgcgcaaaac
     5461 ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc tctctttgtt
     5521 gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca aacacggagg
     5581 aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg ttgcccggaa
     5641 acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc cacgaattcg
     5701 ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt cgacttggta
     5761 gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat cacagcgttc
     5821 gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat gctgccccaa
     5881 tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt ctgctgtttt
     5941 ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga catactggct
     6001 gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga caatagcggc
     6061 catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg ttttctcgcg
     6121 tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca gacacgaacc
     6181 atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa ttgaatcgaa
     6241 ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta cacgccagat
     6301 cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg aaggccacaa
     6361 gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca ataacttcca
     6421 tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac atggtattgc
     6481 tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt ttatggacta
     6541 tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct ttatggacta
     6601 cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta aggtcaccga
     6661 tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg atgcgctatg
     6721 tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac tcgatgccca
     6781 gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg cttttgcgat
     6841 ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg tacgtcaggc
     6901 aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg aagcccgtct
     6961 cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac atgtaaaagg
     7021 cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat gagaaaaatc
     7081 gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa cacaacgcgg
     7141 agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg ggttcgagtc
     7201 ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc tttcgtcgtt
     7261 ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa aaatggccag
     7321 acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc agcagttcta
     7381 ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct gcctggcgca
     7441 gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg atgacacgca
     7501 ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg gtcatcggtg
     7561 atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc tgttcagtat
     7621 gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc tccggcgtgg
     7681 tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag aagtccggtg
     7741 cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc tctgctgcct
     7801 ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt tgctggcgta
     7861 ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc tccacatccc
     7921 gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata tccgccatta
     7981 caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca gcatcttcgg
     8041 cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac gtggagcaca
     8101 acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc tggatacggc
     8161 agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga cgatgggcgt
     8221 cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg ccatattgcg
     8281 cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg tactccagat
     8341 ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc atttaattat
     8401 cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg caccagatgg
     8461 gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc ccgcaaatcc
     8521 tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt atccagaccg
     8581 cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca gcggtgatga
     8641 aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc agttcggtgt
     8701 agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga ctgatgtgca
     8761 ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc agcgcgggcg
     8821 tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc ggaacggtac
     8881 acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag gggggggcct
     8941 gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga caggccaggt
     9001 cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat taatggaaac
     9061 atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga tgccggaaaa
     9121 accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca gtagtccgga
     9181 accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc gcacctggcg
     9241 cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca acgcctggcc
     9301 gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact ggcggatcaa
     9361 ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg ggcggataag
     9421 ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc cattgcgggc
     9481 aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt ggcgcagatt
     9541 ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg agcccgtata
     9601 gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct cccggcgcag
     9661 ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga gcgggcgaaa
     9721 ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg agaacacctc
     9781 ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc tgtgcgtgac
     9841 atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga acaggctgaa
     9901 ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca gcaggataac
     9961 aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg tcggggtgaa
    10021 aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac cgtgaaacac
    10081 gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca taacgggctg
    10141 ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca ggggcgcgat
    10201 ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc cgcagagata
    10261 aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa aggtgcgcag
    10321 ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt gcagggcagg
    10381 catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc acagcgttac
    10441 atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg cggagaaaaa
    10501 ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg tatcgggatg
    10561 caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga ccacttccag
    10621 caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc gggcggtcag
    10681 gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg ttccctgcat
    10741 gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg tttcccgcag
    10801 atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc tctctctgaa
    10861 ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc ggaatgcagt
    10921 cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt tatggccact
    10981 ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca gccggggttc
    11041 aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt gctcatacag
    11101 aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg cgtacggata
    11161 acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg acaaatcccg
    11221 gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc gtttctggtt
    11281 tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc ctggacgctg
    11341 gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt ggacaaatcc
    11401 agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc ctgtaccagt
    11461 gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga gtcggcagcc
    11521 ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc attgttcagg
    11581 gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg cgatttcagc
    11641 gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag ttcgagtacc
    11701 ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt ccactgcccg
    11761 gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc ctgcgcttca
    11821 ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg cggcgggaga
    11881 ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc acagcggatc
    11941 agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc gcatagctgg
    12001 atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa caggctgtgg
    12061 cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg aatgccctcc
    12121 accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt attgtttgtc
    12181 atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa tgccaatgat
    12241 gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt ccggggcgta
    12301 ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc agatagtttc
    12361 atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc caccagtgac
    12421 catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca gatcctgcga
    12481 ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt gttcgggaga
    12541 taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt tttccgcacc
    12601 ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt gtgatgtcag
    12661 atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg acgtgatggc
    12721 agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc catgtacaag
    12781 gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca tacggtgatg
    12841 aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat tgataatcca
    12901 ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat tcaccttaaa
    12961 aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag tcgcgtaaat
    13021 tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa tatttggttt
    13081 cattgtgtat agactggccc cctgaatctc cagacaacca g
//
LOCUS       DAJTRH010000098        12762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTRH010000098 DAJTRH010000000
VERSION     DAJTRH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(270..989)
                     /locus_tag="OO339_003180"
                     /pseudo
     CDS             complement(270..989)
                     /locus_tag="OO339_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1005..1337)
                     /gene="cusF"
                     /locus_tag="OO339_003181"
     CDS             complement(1005..1337)
                     /gene="cusF"
                     /locus_tag="OO339_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5927704.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1361..2743)
                     /gene="cusC"
                     /locus_tag="OO339_003182"
     CDS             complement(1361..2743)
                     /gene="cusC"
                     /locus_tag="OO339_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5927705.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2900..3583
                     /gene="cusR"
                     /locus_tag="OO339_003183"
     CDS             2900..3583
                     /gene="cusR"
                     /locus_tag="OO339_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5927706.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3573..5015
                     /gene="cusS"
                     /locus_tag="OO339_003184"
     CDS             3573..5015
                     /gene="cusS"
                     /locus_tag="OO339_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5927707.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5165..7402
                     /gene="nrfB"
                     /locus_tag="OO339_003185"
     CDS             5165..7402
                     /gene="nrfB"
                     /locus_tag="OO339_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5927708.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7389..10357
                     /gene="nfrA"
                     /locus_tag="OO339_003186"
                     /pseudo
     CDS             7389..10357
                     /gene="nfrA"
                     /locus_tag="OO339_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10358..11248
                     /locus_tag="OO339_003187"
     CDS             10358..11248
                     /locus_tag="OO339_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5927709.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11431..12192
                     /gene="envY"
                     /locus_tag="OO339_003188"
     CDS             11431..12192
                     /gene="envY"
                     /locus_tag="OO339_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5927710.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12238..12314)
                     /locus_tag="OO339_003189"
     tRNA            complement(12238..12314)
                     /locus_tag="OO339_003189"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12278..12280),aa:Arg,seq:tct)
     gene            12557..>12762
                     /locus_tag="OO339_003190"
     CDS             12557..>12762
                     /locus_tag="OO339_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5927711.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3124 a         3309 c         3238 g         3091 t
ORIGIN      
        1 attcttgtgg gccagaatat ggtttaccat aaatagtacg cagttttgtg tcgcttatag
       61 ccataaataa tatttggtac gctcagaaat aatggtttgg tacacatctg gtacacaata
      121 tcacatggac aacagcgcac agtaaacaac tatatttgac gttcgtagac atactcaggt
      181 gaaaaaaagg ttgtttttcg agttataaca gacagttaat taactacagt ggacaatgct
      241 agacaatgac agacacagat aagcaaccta ccttcctctt tcgcgaccac gttatctttg
      301 gcgatattca ttcccgcgcg cagatcaaac gcggtgatca cgccatcaat gggcgctttg
      361 agcgtaaagc gagtctggat tttttgcgtg gcgatcaggt ggcgaatatc cgcctccggc
      421 attcccgcca gtcgcagccg ctcaagaatg ccttcagtct gggtcgccgt accgccggtt
      481 tcgcgcaaca gtaaatactc actctgcgct tccacccagt cgggaatggt caggtcgaga
      541 agcggtgtgc ccttttgtac tttatcgccc acggtaagcg gatacacctt gtcgataaac
      601 ccggcagcgc gagcctgcac aatggcatac tgatactcgt tgtaactgac attcgtcggg
      661 aaactctggg caaaagtcag cggcccgcgc gtgacggtag ccgttttcac ccccagattc
      721 tgcgtctgag tcgggtcaat gcgcacacca gacgcagaac tctcttcatc ggcatatttc
      781 ggcaccagat ccatatccat aaacggcgat ttacctggtt tatcgaaccg cgtattggga
      841 tacatcgggt cgtaccagaa taagatttta cgttctgcgg tcggcatttt ttctgcgtgc
      901 ggttccgcct ggtcaaacca ggtaaaacct gccgctgaaa taataccgcc cgcgatcatg
      961 ctgccgataa taagcgcgat ttttttcatc tcattaaacc tgggttactg gctgacttta
     1021 atatcctgta ataaagaaag gttgccctgc tggacaaaat taaacgccac tttgtcgccg
     1081 gttttaattt cactcatttt cgtctgcggg gtgatggtaa agcgcatggt catctccggc
     1141 cagttgacgg cagcaatcgg atcgtgatgg atggtgattt ttttgctttc cagatcaaca
     1201 ccttttacca caccggtggc actaatgacc tgtggttgtg cttcgctcat ggtttcatga
     1261 tgatgttcgt tagcctgggc attaaagcca ataacggtaa acagactgaa cattgcgact
     1321 tgcagtgctt ttttcattta ttctctcctg gagttaaaaa ttattgctgc caaccgcccc
     1381 ctaaggcggt atacaaataa atttcgttaa cctgacgggc ataattcagg tcaagtaaag
     1441 tttgtcgggt tgcaaataaa gaacgctcgg catccagcac ttccagataa cttaccgcgc
     1501 cgtgctgata taatgcccgc gcccgttgca gggtaatttg cagcgacgcc agataacgct
     1561 gctgggcgct gatttgatcg tccaggcttt gacgtaatgc cagcgcatct gccacctctt
     1621 taaaggcgtt ctggattttc tgttcataat tcaccaccga ctgctgctgg cgaatttcgg
     1681 cgatatccag attggcctgg ttgcgtccgg cattaaaaat ggggatctca attttaggaa
     1741 taaaattcca catcccgctg ctagcgttaa ataatgacga cagatcgctg ctggcggttg
     1801 atattccgct ggtcaggctg atagacggga aaaatgccgc acgcgcagca ccaatattag
     1861 cattagccgc cattaacgcg tgttcagctt ccataatatc cgggcgctgc aataagattt
     1921 gcgacgacaa gcccgccggt aatttaacgc tttgcaggct gccgctgttt actgtctgcg
     1981 cttgcggtag cttgccgtag cttcccaata acagttgcaa tgcattattc gcctgcgcca
     2041 gttccccctg acgtttagcg atgtcactgc gggtactttc tatcaccccg cgagcctgtt
     2101 ccagcgccag aacattgctg ctaccggtca acagttgttt ttcgacaaac gcatatgact
     2161 gctgataatt acgcagcgtt tcttcggcta tttgcaattg cgcatacgcc agctgctgat
     2221 tgaaatagct ttgcgcgaca ttagaaacca gcaaaatatg caccgcgcgc tgggcttcct
     2281 cagtggctaa ataattttgg cgctcggctt cgctcatgtt ctttaagcga ccgaaaaaat
     2341 cgagatcaaa gctggcgtta aggccagtcg agaactcccg cgtcgtggct gaatcgcctt
     2401 taagattgcc gctccagctg ccgctgccct cgccattgag ctgtgggtag cggtcggcat
     2461 cggtcagacg atattgcgcc cgtgcctcct gcactttcag cgtcgccatg cgcaaatccc
     2521 ggttattcac cagcgcctcg ctaatcagcg tcttcacctg attatcaaca aaaaaggtgc
     2581 gccagcccac gttctggtag ttatccgctg cgttaaccag gccgttctgg ctgagtgaga
     2641 actgctgcgg tacgggcatt accggacgct gataatccgg tgccagtgaa caaccagtta
     2701 gcgcaagggc cacacaaaat ggcagaagtt tacaaggaga cataggctca taatttctgg
     2761 tgattttatg tcgccaactt tactcgccag gctctgattt tccggtgaca ggaaaatgac
     2821 aaaattgtca ttttgccaat aagcgattgc catctgatcc cgctactcta gaattgcccg
     2881 ggcaacatgc ggaggaaata tgaaactgtt gattgtcgaa gatgaaaaga aaaccggaga
     2941 atacttgacc aaagggttaa ccgaagccgg ttttgtggtc gatttggccg acaacgggct
     3001 gaatggctac catctggcga tgaccggtga ttatgacctg ataatcctcg atattatgct
     3061 gccagacgtg aacggctggg atatcgtgcg catgttgcgc tccgccaata aagggatgcc
     3121 gattctgttg cttaccgcgc ttggcaccat tgaacatcgc gtcaaggggc tggagttggg
     3181 ggcagatgac tacctggtga aaccgttcgc ttttgctgaa ctgctggcgc gggtgcgcac
     3241 tctcctgcgg cgcggggcgg cggtgattat cgaaagtcag tttcaggttg ccgatctgat
     3301 ggtcgatctc gtcagccgca aagtcacccg cagcggcacg cgcatcactc tgaccagtaa
     3361 agaatttact ctgctggagt tttttctccg ccatcagggc gaagtgctgc cccgctcgct
     3421 tatcgcctcg caggtatggg acatgaattt cgacagcgac actaacgcca ttgatgtggc
     3481 ggtgaagctg ctacgcggca aaatcgacaa cgactttgag ccgaagctga ttcaaaccgt
     3541 gcgcggcgtg ggttacatgc ttgaggtgcc ggatggtcag taagccattt cagcgcccgt
     3601 tttcgctggc aacccgcctg acctttttta tcagccaggc caccatcgcg gcgtttttcg
     3661 cctttgcatg gatcataatc cactcagtaa aagtgcattt tgccgagcag gacattaatg
     3721 atttaaaaga gattagcgcc acccttgaac gggtactaaa tcaccctgac gaaacgcaag
     3781 cccgacgctt aatgacgctg gaagatatcg tcagtggtta ttccaacgtg ttgatttccc
     3841 tggcagatag ccacggcaaa acggtgtatc actcccccgg tgcgcctgat atccgcgagt
     3901 ttgcacgtga tgccataccc gataaagacg cgcggggtgg cgaggtgtat ctcctttccg
     3961 gcccgacgat tatgatgcca ggccacggtc acgggcatat ggaacacagc aactggcgga
     4021 tgattaactt gccggttggc ccgttggtgg acggcaaacc gatttatacg ctctacatcg
     4081 cgctgtcgat cgattttcat cttcattaca taaatgattt gatgaataaa cttattatga
     4141 ccgcatcggt aatcagcatc ctgatcgtct ttatcgtgct gttggcggta cataaaggtc
     4201 acgcgccgat ccgcagcgtc agccgtcaaa tccagaatat cacctcgaaa gatctcgacg
     4261 ttcgccttga cccgcagacc gtgccgattg agctggaaca gctggtactg tcgttcaacc
     4321 atatgatcga gcgtattgag gatgtcttta ctcgccagtc caatttctca gcggatatcg
     4381 cccacgaaat tcgcacacca attactaatc tcattacgca aacggaaatc gccctcagcc
     4441 agtcgcgcag ccagaaggag ctggaagatg tgctctactc taatctcgaa gagctgacgc
     4501 gaatggcgaa aatggtcagc gatatgctgt ttctcgctca ggccgataac aaccagctaa
     4561 tccccgaaaa gaaaatgctc aacctggcgg atgaagtcgg caaagtgttc gattttttcg
     4621 aggcgctagc ggaagatcgc ggcgtggagt tgcggtttgt tggcgacaaa cgtcaggttg
     4681 cgggcgatcc gttgatgctg cgtcgggcgc taagcaacct gctttctaat gcccttcgtt
     4741 atacgccaac cagagagaca attgtagtac gctgtcagac ggtcgatcac caggtgcaag
     4801 tttccgtcga aaaccccggt acgcccattg cgcccgagca cttaccgcga ttgtttgacc
     4861 gtttctatcg cgttgacccc tcccgccagc gaaaaggtga aggtagcggt attgggctgg
     4921 cgatagtgaa atcgattgtt gtcgcgcata aaggcacggt tgcggtaacg tcagatgcgc
     4981 gggggacaag gtttgttatc acattacccg cttaattatt cagcaaacgg caacttttat
     5041 aaccagtgta aaaataacgt gccgcaatag tcatacatat taatggtaaa aagctgtcac
     5101 aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa tgtaaggttt
     5161 ttgtgtggac tggcttcttg atgtttttgc tacctggctc tacggcttaa aagtaatcgc
     5221 gataacgtta gcggtcatca tgttcatcag cgggctggac gattttttta ttgatgtcgt
     5281 ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc cgcgaatgag
     5341 ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc cggcgtggaa
     5401 tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg actacgaaaa
     5461 ctatcatatc tttgttggca cctaccccaa cgaccccgat actcaacgtg atgttgacga
     5521 agtgtgcgct cgctttccga acgtgcataa ggtagtctgc gcgcgtcctg gtcccaccag
     5581 caaagccgac tgcctgaaca acgtgctgga cgccatcacc cagtttgaac gtagcgccaa
     5641 tttcgctttt gctggtttta ttctgcatga cgccgaagat gtgatttcac cgatggaatt
     5701 gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccag tgtatccgtt
     5761 tgaacgcgaa tggacgcact tcaccagcat gacttacatt gatgagtttt cagaactgca
     5821 tggcaaagat gttccggtgc gtgaagccct cgccggacaa gtgcccagcg caggcgtcgg
     5881 cacctgtttc agccgccgcg ccgtgaccgc actgttagct gacggtaacg gtattgcttt
     5941 cgacgtgcag agtcttactg aagattacga cattggcttc cgcctgaaag aaaaaggtat
     6001 gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg agcagcgtaa
     6061 atttttacag cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt tccccgatac
     6121 cttttcgact gcggttcgac aaaaatctcg ctggatcatc ggcattgttt tccagggctt
     6181 taaaacccat aaatggacct ccagcctgac gctgaactac tttctctggc gcgaccgcaa
     6241 aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgatcc agcttttgct
     6301 gttgctggcg tatgaaagtt tgtggcccga tgcctggcat ttcctttcta tttttagcgg
     6361 cagcgcatgg ttaatgaccc tgctgtggct aaactttggc ttgatggtta accgcatcgt
     6421 gcagcgagtg attttcgtca ctggctacta cggcctcacg cagggcctgc tttccgtcct
     6481 gcgtcttttc tggggcaacc tgattaactt tatggctaac tggcgcgtgc tgaaacaggt
     6541 tcttcagcac ggcgatccgc gtcgcgtcgc gtgggataaa acaacgcatg acttccccag
     6601 cgtcactggc gatacccgct cgttgcgccc gttaggtcaa attctgctgg aaaatcaggt
     6661 catcactgaa gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc tacgcctggg
     6721 cggttcaatg ctgatgcagg ggctgattag cgccgagcag ctggcacagg cgctggcaga
     6781 gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccgtcct cgctgattgc
     6841 cgaaatgcca gcctccgtag cgctgcatta tgcagtactg ccgctgcgtc tggaaaatga
     6901 tgagttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg ccctgacgcg
     6961 taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg tcacagggtt
     7021 acgccactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt acaatgcggt
     7081 tcagcatcag tggctcacgg aacagcagac cggtgaaatc tggcggcaat atgtgccgca
     7141 tcagttcctg ttcgccgaaa tactgaccac gctcggtcat attaatcgtt cagcaattaa
     7201 cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt tggtcaccga
     7261 aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg aattacaagt
     7321 ttcgatgcaa tcactattac tcaaagcagg tttaaacaca gaacaggttg cgcaactgga
     7381 gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt ttactgctga
     7441 cgtctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca gaagagctgg
     7501 ggctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg ctgaaggcac
     7561 agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac caacaggtgc
     7621 cggataatat tccgctgact ttataccttg cggaagccta tcgtcatttt ggtcatgatg
     7681 accgggcgcg gctgttgctt gaggatcaac tgaaacgtca cccaggagat gcccgacttg
     7741 agcgcagtct ggcggctatt ccagttgaag tgaaaagcgt tacgactgtt gaagaactgc
     7801 ttgcccagca aaaagcgggc gacgctgcgc cgaccctgcg ttgtcgcagt gaagtcgggc
     7861 agaatgccct gcggctggca caattacctg tcgccagagc gcaactgaac gatgcgacgt
     7921 ttgctgcatc gccggaagga aaaacgctgc gaaccgatct gctgcaacgg gcaatctacc
     7981 tgaaacaatg atcccaggca gatacgctat acaatgaagc acgccagcag aacacattaa
     8041 gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc cgggcagctg gacgatcgga
     8101 tcctggcact gcaatcacag gggatcttca ccgatcctca gtcatatatt acttacgcga
     8161 ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcatt gaaaataagc
     8221 cgctgtttac cacgggcgca caagagaaaa gttggctcta tctgttatct aaatacagcg
     8281 ccaaccccgt tcaggcattg gcgaattata cggtgcagtt tgccgataac cgccagtatg
     8341 ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacgacgca gcgcaaaaac
     8401 tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc agcgtggcga
     8461 cccgtaacaa ggctgaatct ctgcgtctgg cacgattgct gtatcagcaa gaaccgacaa
     8521 atcttccccg cctggatcaa ctgacctggc aactgatgca gaacgagcag tcacgcgaag
     8581 ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtgtc agccagactt
     8641 taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctagcaacg ccggcgaagg
     8701 tggcgatttt atcgaaaccc ttaccgctgg cggagcaacg tcagtggcaa agtcagttgc
     8761 cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg tcgccttcct
     8821 acgatgctgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta cccggtgtgg
     8881 cgttgtatgc atggcttcag gccgaacaac gacaaccgag cgcctggcaa catcgtgcgg
     8941 tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc tggcagaaaa
     9001 tcagtcttca cgacatgagc aatgatgatc tgctcgccgc cgccaacacc gctcaggcag
     9061 cgggaaatgg cgcagctcgc gatcgctggc ttcaacaggc agaacaacgt gagctgggaa
     9121 acaatgctca atactggtgg ctgcatgcgc gacgttatat cccagctcag ccagaactcg
     9181 cactgaacga tctcacgcgc tcaatcaata ttgccccttc tgccaactct tacgttgcgc
     9241 gggcgacaat ttatcgccaa cgtcataatg ttccggccgc ggtgagtgat ttgcgcgccg
     9301 cgctggaact ggaaccaaat aatagcaaca cccaggcagc gcttggttac gccttgtggg
     9361 atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa gggcttccgg
     9421 acgatccggc actgatccga caactggcct acgtgaacca gcgtctggat gacatgcctg
     9481 cgacgcagca ctacgcccgg ctcgtgattg atgacattga taatcaggca taaccccact
     9541 gaccccagag caaaatcagc aacgcttcaa tttccgccgt ttgcatgagg aggtcggtcg
     9601 ccgctggacg ttcagtttcg attcttccat cggcttgcgt tccggcgcaa tgagtaccgc
     9661 taacaataat gtcggcggtg cagcgcctgg gaaaagctat cgtagctacg gacaactgga
     9721 agccgagtac cgcatcggac gcaatatgct gctggaaggc gacctgctct cagtttatag
     9781 ccgcgtcttt gccgataccg gagaaaacgg ggtgatgatg ccggtgaaaa atccgatgtc
     9841 cggcaccggt ctgcgctgga agccgctgcg cgatcagatc tttttcctcg ccgtcgaaca
     9901 gcagttgccg ctgaacgggc aaaatggcgc atccgatacc atgctgcgcg ccagcgcctc
     9961 attctttaat ggcggcaaat acagcgacga atggcacccg aacggttcag gctggtttgc
    10021 ccaaaacctg tacctcgatg cggcgcaata tatccgccag gatattcagg cgtggacggc
    10081 agattatcgc gtcagctggc atcagaaggt agctaacgga cagactattg agccttacgc
    10141 tcacgttcag gacaacggct atcgtgataa aggcactcag ggcgcgcagc ttggcggtgt
    10201 cggggtccgc tggaatattt ggaccggcga gacgcactac gacgcctggc cgcacaaagt
    10261 cagtctcggc gtcgagtatc aacatacctt taaggcgatt aatcaacgta acggagagcg
    10321 caacaacgcg tttctcacca ttggagtgca ctggtaaatg cgtaagttca ttttcgtatt
    10381 gctgacactg cttttggtca gccctttttc ctttgcgatg aaaggtatta tctggcaacc
    10441 acaaaaccga gatagtcagg ttaccgatac ccagtggcag gggctgatga gtcagttacg
    10501 ttttcaaggc ttcgataccc tggttttgca atggacccgt tacggcgatg catttaccca
    10561 gccagaacag cgcacgttat tgtttaagcg tgccgcagct gcgcaacaga ctggcctgaa
    10621 gctgattgtc gggctgaacg ccgatccgga attttttatg caccagaaac agtcgtctgc
    10681 agcgctggaa agctatctta atcgcctgct ggctgccgat ctccagcaag ccagattatg
    10741 gagcgccgcg cctggcataa cgccggatgg ctggtacatc agcgcggaaa ttgacgacct
    10801 gaactggcgc agcgaagccg cccgtcaacc tttgctaaca tggttaaaca acgcgcagcg
    10861 gctgattagc gatgtttcag caaaaccggt ttatatcagt agttttttcg ccggaaacat
    10921 gtcgcccgat ggctatcgcc aactgctgga acaagttaaa gcaaccggag ttaatgtctg
    10981 ggtacaggat ggcagcggcg tggataaact taccgctgaa cagcgtgaac gttatctaca
    11041 ggccagcgcc gattgccaaa gctccgcccc agcaagcggc attgtttatg aactgtttgt
    11101 cgccggcaaa ggcaaaacct ttacagcgaa accgaaaccg gacgcagaaa ttgcctcgct
    11161 gttagcgaaa cgttcctctt gcggtaaaga catgctctat ttctctctgc gctatttgcc
    11221 cgtcgcgcac ggcattctcg agtattaaat cccctccagg taagtcgggt acgacctggc
    11281 ttacctcttt cgctctcgtc agaattccat caagattaat tgctaaaaag catgctaaag
    11341 aaagaagatc gttatcggcg aacgggccga tcactcatgc aggaagcata gatcctttga
    11401 gcggagtttc tgatatctga ggagtgcgaa atgcaattga gcagcagtga accttgcgtg
    11461 gtgatcctga ccgaaaaaga ggtagaggta agcgtcaata accatgctac gtttaccctt
    11521 ccgaaaaact acctggccgc cttcgcgtgc aacaataacg tcattgaact ctcaacgtta
    11581 aatcacgtat taatcaccca catcaaccgt aacatcatca acgattatct gttgttttta
    11641 aataagaaat taacctgtgt aaagccctgg tcgcggctag caaccccggt tatcgcttgt
    11701 catagccgta caccggaagt gttccggcta gccaccaacc acagcaagca gcaatccagc
    11761 aaaccctgcg aagcggagtt gacgcgcgca ttacttttta ccgtattgtc taactttctt
    11821 gagcaatcgc ggtttattgc cctactgatg tatatcttac gcagcagcgt ccgcgacagc
    11881 gtctgccgca ttattcaaag cgatattcag cattactgga atctgcgaat tgtcgccagt
    11941 tcgctatgtt taagccccag cctgctcaaa aagaaattaa aaaacgaaaa taccagctat
    12001 agccagattg tcacagagtg tcgtatgcgt tacgccgtac agatgttatt gatggataac
    12061 aaaaatatca ctcaggtggc gcaattatgt ggctatagca gcacgtcgta ctttatctct
    12121 gtttttaagg cgttttacag cctgacaccg ttgaattatc tcgccaaaca gcgacaaaaa
    12181 gtgatgtggt gaagggcaaa gcggaaacgg ataagacggg cataaatgaa gaagaaatgg
    12241 cgcgccctgc aggattcgaa cctgcggccc acgacttaga aggtcgttgc tctatccaac
    12301 tgagctaagg gcgcgttgat accgcaatgc ggtgtaatcg cgtgaattat acggtcaacc
    12361 cttgctgagt caatggcttt tgatctggtt gctgaacaag tgaacgaccg cgtctgattt
    12421 tctgatttat ttcgctatag cggcaaacaa acgcacaccg ctgcgcgtct gaatcaagaa
    12481 aacccgtatt ttcatgtatc aaagtacaat ttaccgccct aacggaaaat tgtccgctcc
    12541 tatgagactg gtaactatga aaccaacgtc ggtgatcatt atggatactc atcctatcat
    12601 cagaatgtct attgaagttc tgttgcaaaa aaacagtgaa ttgcagattg tcctgaaaac
    12661 ggatgattac cgcataacca tcgattatct ccgaacccgt cctgttgatt taatcattat
    12721 ggatatagac ttgcccggaa cagacggttt taccttcctg aa
//
LOCUS       DAJTRH010000099        12624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTRH010000099 DAJTRH010000000
VERSION     DAJTRH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            758..841
                     /locus_tag="OO339_003191"
     CDS             758..841
                     /locus_tag="OO339_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5927712.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO339_003192"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO339_003192"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5927713.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO339_003193"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO339_003193"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5927714.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="OO339_003194"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="OO339_003194"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5927715.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO339_003195"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO339_003195"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5927716.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO339_003196"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO339_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO339_003197"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO339_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5927717.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="OO339_003198"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="OO339_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5927718.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO339_003199"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO339_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5927719.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="OO339_003200"
     CDS             10967..12166
                     /locus_tag="OO339_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5927720.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3106 a         3167 c         3356 g         2995 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggtgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttat
//
LOCUS       DAJTRH010000100        12604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTRH010000100 DAJTRH010000000
VERSION     DAJTRH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            70..1767
                     /gene="maeA"
                     /locus_tag="OO339_003201"
     CDS             70..1767
                     /gene="maeA"
                     /locus_tag="OO339_003201"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5927721.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1901..2911
                     /gene="adhP"
                     /locus_tag="OO339_003202"
     CDS             1901..2911
                     /gene="adhP"
                     /locus_tag="OO339_003202"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5927722.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3057..3341
                     /locus_tag="OO339_003203"
     CDS             3057..3341
                     /locus_tag="OO339_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5927723.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3569..4222)
                     /gene="fdnI"
                     /locus_tag="OO339_003204"
     CDS             complement(3569..4222)
                     /gene="fdnI"
                     /locus_tag="OO339_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5927724.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4215..5099)
                     /gene="fdnH"
                     /locus_tag="OO339_003205"
     CDS             complement(4215..5099)
                     /gene="fdnH"
                     /locus_tag="OO339_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5927725.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5112..8159)
                     /gene="fdnG"
                     /locus_tag="OO339_003206"
     CDS             complement(5112..8159)
                     /gene="fdnG"
                     /locus_tag="OO339_003206"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7572..7574),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5927726.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8391..9272
                     /gene="yddG"
                     /locus_tag="OO339_003207"
     CDS             8391..9272
                     /gene="yddG"
                     /locus_tag="OO339_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5927727.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9527..10627
                     /gene="ompC"
                     /locus_tag="OO339_003208"
     CDS             9527..10627
                     /gene="ompC"
                     /locus_tag="OO339_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5927728.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10791..11276
                     /locus_tag="OO339_003209"
     CDS             10791..11276
                     /locus_tag="OO339_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5927729.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11386..12375
                     /locus_tag="OO339_003210"
                     /pseudo
     CDS             11386..12375
                     /locus_tag="OO339_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT         3328 a         3116 c         2927 g         3233 t
ORIGIN      
        1 acgccctggc ggtaaagcaa agacgataaa agcgtgccag ggatggatat tcaaaaaaga
       61 gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc ttacgctggc
      121 cctgtactgc tggaatttcc gttgttgaat aaaggcagtg ccttcagcat ggaagaacgc
      181 cgtaacttca acctgctggg gttactgccg gaagtggtcg aaaccatcga agaacaagcg
      241 gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca catctacctg
      301 cgtaacatcc aggacaccaa cgaaaccctc ttctaccgtc tggtaaacaa tcatcttgat
      361 gagatgatgc ctgttattta taccccaacc gtcggcgcag cctgtgagcg tttttctgag
      421 atctaccgcc gttcacgcgg cgtgtttatc tcttaccaga accgccacaa tatggacgat
      481 attctgcaaa acgtgccgaa ccataatatt aaagtgattg tggtgactga cggtgaacgt
      541 attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg taaactgtcg
      601 ctctataccg cctgtggcgg catcagcccg gcgtataccc ttccggtggt gctggatgtc
      661 ggaacgaaca accaacagct gcttaacgat ccgctgtata tgggctggcg taatccgcgt
      721 atcactgacg atgaatacta tgaattcgtt gatgaattta tccaggctgt gaaacaacgc
      781 tggccggacg tgctgttgca gtttgaagac tttgctcaaa aaaatgcgat gccgttactt
      841 aaccgctatc gcaatgaaat ttgttctttt aacgatgaca ttcagggcac tgcggcggta
      901 acagtcggca cactgatcgc agccagccgc gcggcaggtg gtcagttaag cgagaaaaaa
      961 atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat catcgcccag
     1021 acccagcgcg aaggattaag cgaggaagca gcgcggcaga aagtctttat ggtcgatcgc
     1081 tttggcctgc tgacggacaa gatgccgaac ctgctgcctt tccagaccaa actggtgcag
     1141 aagcgcgaaa acctcagtga ctgggatacc gacagcgatg tgttgtcact gctggatgtg
     1201 gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg gctgtttacg
     1261 gaagagatca tccgtgagat gcataaacac tgtccgcgtc cgatcgtgat gccgctgtct
     1321 aacccgacgt ctcgcgtgga agccacaccg caggacatta tcgcctggac cgaaggtaac
     1381 gcgctggtcg ccactggcag cccgtttaat cctgtggtat ggaaagataa aatctaccct
     1441 atcgcccagt gtaacaacgc ctttattttc ccgggcatcg gcctgggtgt tattgcttcc
     1501 ggcgcgtcac gtatcaccga tgagatgctg atgtcggcaa gtgaaacgct tgctcagtat
     1561 tcgccgctgg tcctgaacgg cgaaggtctg gtactaccgg aactgaaaga tattcagaaa
     1621 gtctcccgcg caattgcgtt tgcggttggc aaaatggcgc agcagcaagg cgtggcggtc
     1681 aaaacgtctg ctgaagcttt gcaacaagcc attgacgata atttctggca agccgaatac
     1741 cgcgactacc gccgtacctc catctaagcc tgcacccggt agtgaaggct accgggctat
     1801 ttccctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa tctcatttgc
     1861 ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaagactg cagttgttac
     1921 gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac atggcgaagc
     1981 cctgctgaaa atggagtgtt gtggtgtatg tcataccgat cttcatgtta agaatggcga
     2041 ttttggtgac aaaaccggcg taattctggg ccatgaaggc atcggtgtgg tggcagaagt
     2101 tggcccaggt gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt ggttttacga
     2161 aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc gttcagttaa
     2221 aaatgccgga tacagcgttg atggcgggat ggaggaagag tgcatcgtgg tcgccgatta
     2281 cgcggtaaaa gtgccagatg gtctggactc ggcggcgccc agcagcatta cctgtgcggg
     2341 ggtcaccacc tacaaagccg ttaaactgtc aaaaattcgt cccggtcagt ggattgctat
     2401 ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcaaagaatg tctttaacgc
     2461 caaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatggatgc
     2521 agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg agaaaaccgg
     2581 tggcgctcac gctgcggtgg taacagcagt agctaaagct gcgtttaact cggcagttga
     2641 tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt ctatgagcct
     2701 ggatatccca cgtcttgtgc tggatggcat tgaagtggtc ggttcgctgg tcggcacgcg
     2761 ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc
     2821 cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg
     2881 tggccgtatg gtgattgatt tccgtcacta agaggtcttt gctgcgactc ccatgttcgg
     2941 gtcgcagcat cgcacactct ccaacatgaa atggctaaat ggattattgt tgcgtaggaa
     3001 ggaacaataa tgatagtgtt cccagagaag taacgctact gagagtaaag acactaatga
     3061 aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac gaacttaatg
     3121 tcagcctgcg cgagtttgcc agagcaatgg aaattgcgcc ctcaacggca agccgattgc
     3181 tgaccggaaa agcagctttg acgccagaaa tggcaattaa actttccgtg gtgatcggca
     3241 gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag gcagaaaaaa
     3301 cggtggttgt gtcgcgatta cgccgtctgg ttacgcaata aaaaatggca ccctataaag
     3361 cgccatttga aactgatgac aaacgcaaca ttgcctgatg cgctacgctt atcaggccta
     3421 cacgttcact tcaatctatt gtatttgaag ggttttcatg ccggataagg cattcacgac
     3481 gcatccggga tgaacaaagc ccacgttgtc tacaatatga aaatggcgcc ttttacagcg
     3541 ccattttcag cttaaagttc aaaagagatt atatcccttc ttcactctct tttttcgctt
     3601 ctgccttctc gatttcacga taccagcgcg gatggtgttt cttcgcccag cgacggctca
     3661 ccttcccttc gatcatccct ttaatcgacc ctttcaccca gaatgccata tacatatgga
     3721 tcaggatggc gtggatcagg atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa
     3781 caacctgcat cgggaagtac tgcgcaaagt acggacgcca gataatcacc ccggtcacca
     3841 gcagcacgaa aatcatgctc atgatcgacc agaacatcat cttttgcccg gcgttgtact
     3901 taccaacatc cgccactttg tgctcattgc cttttaatac ttcgacaatg ttcaacagcc
     3961 acggaatatc tttcttatcc gggatgttgt gatgcacaaa acgcacaaac ataaacatca
     4021 gtgcgacgaa aatcgcaatg ccgaagaagg ggtgcaaaat gcgtcccatc tgcggcgtac
     4081 cgaaggtttg cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca
     4141 ccaggaagaa gcaaatcacc acggtccagt gacaggcgcg atcaataaat ttggtgcgca
     4201 caatcatttt cgacttactc atgatgatcc tcctcgtcat cgtccacttc cttattcggg
     4261 ccaataccga tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc agccgctgcc
     4321 agcggtttca acgcgccttt ccacaggctt accgaggtgt cgatcttcgg atctttcggc
     4381 agaccgtgat acagctccgg ctgatcggcg tgatgcagca cgtacataac gtgcgtacca
     4441 ccgacccctt ccgggttgta gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc
     4501 acgcgctgtt ccgccagctc cagcatctcc ttcttggtgc cgaagtggat agccccggtc
     4561 ggacaggttt tcacacaagc cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg
     4621 catttatata cccggttatc ctctttgttg aggcgcggaa tattaaacgg acacccggca
     4681 atgcagtaac cacagccgat gcagttttcc gactggaaat cgacaatccc gttagcgtac
     4741 tgaatgattg caccagcaga cgggcacgcc ttcaggcagc ccggatcttc acagtgcata
     4801 cagccgtctt tacggatcag ccactccagc ttgccattct gttcggtttc gctaaagcgc
     4861 atcaccgtcc aggacttggc gctcagatcg gcggggttat cgtaaacccc gacgcagtgc
     4921 cccacttcat cacggatgtc gttccactcc gaacacgcca cctgacaggc tttacagccg
     4981 atacaggtgg aaacgtcgat aagtttcgcg acttctgctt tgtaatcacg cacctgagaa
     5041 ggcggcgtga tggagttagt tgcggacctt ttgataatat cctgcgtttc catagccatc
     5101 tgttcgcccc cttacgcctt ctcgatgttg actaagaacg ctttatattc cggcgtttgc
     5161 gagtttgcat caccgacatt cggcgtcaga gtgttagcga tataaccttt acgcgcgaca
     5221 ccctcaaagc cccagtggat tggaataccc accgtttcaa cctgctgacc gtttacattc
     5281 agcggtttca gacggcgcgt taccactgcc accgcgcgga taaagcctcg cttgctggag
     5341 acagtgacac gatcgccatt attaatgcct ttcgccgccg cctgcgtttc gctgatttcc
     5401 acaaactgtt ccggctgagc aattgcgttg agcaacgcgt gcttggtcca ggtgtggaag
     5461 tgctcggtca gacgataggt cgtacccaca tacgggaact gctctttttt acccatccgc
     5521 agcgcgtctt gttcatacag acgaacaacc gggttagaca ccacgttcgg atgcagcggg
     5581 ttagtgccca gcggcgtttc aatcggctcg tagtgttccg ggaacggacc ttccgccatt
     5641 ttgttgatgg caaacaggcg tcccatccct tccggctgca taataaacgg cccggttggc
     5701 gtacccggtg cggcattgcc gaagtccgga atatcgttac ccgtccactt gctgccgttc
     5761 cactggatca gcatccgttt cggatcccac ggtttaccgt tgatatccgc cgaagcacgg
     5821 ttgtagagta cgcgacggtt gagcggccac gcccaggccc atcccagcgt attccccaga
     5881 ccggacgggt cggagttatc gcggtttgcc atctggttac cctgctctgt ccagctaccg
     5941 gtgtagatcc agcaagaaga tgcggttgta ccgtcatcac gcagatgcgc aaagctactc
     6001 agcaactgac ctttcttcgc aatcagcacg ccattagcat catagagatc ttccaacgca
     6061 tagccgttgt tctctttagc tacttcgtca gattgcggtt cgtgcggctg cttgtagttc
     6121 cagctcatct tcatcagcgg ttctacgcct ttaccacctt cggcctggta caactcgcgc
     6181 agatgatggt agatacccgc cagaatttcg ccgtcgttac gcgcttcgcc cggcgcgtcc
     6241 tgacctttcc agtgccactg caaccagcga ccggagttag cgatagaacc atcctcttca
     6301 gcaaagcagg tcgaaggcag acggaatact tcagtctgaa tagacgccgg atcgacatcg
     6361 ttcgactcgc cgtggttctg ccagaaggta gaggtttcag tcaccagcgg atcgataacc
     6421 accatgtact tcagtttgct caggcagctc accactttgt ttttatccgg gaaggacgca
     6481 accgggttaa agccctggca gaaataaccg gtaactttgc cttcatccat catgttgaaa
     6541 tacttgatga cgtcgtaggt ctggtcccac ttcggcagcc agtcatagcc ccagttgttc
     6601 tctttctgcg cggcatcgcc atagaaagat ttcatcaggc taacgaagaa cttcggatag
     6661 ttgctccagt agttcacctg atcagccagc gtcgctttcg gcgtgttcgc ttccagatac
     6721 gactgcaaat caacctgttt ttctgacggc agcgtcagat aacctggcag gctggtagag
     6781 agcagaccta agtcagtcag cccctgaatg ttggagtgac cacgcaatgc gttcacgccg
     6841 ccaccggcca tacccatgtt accgagcagc aactggatca tcgccatagt acggatgttc
     6901 tgcgcaccca cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc
     6961 ggtgcgctgg tggaggccag cacttcacac actttcagga agtcggcttt tggcgtacca
     7021 cagatgtttt caacaacgtc cggcgtgtag cgggaaacgt gcgctttcag caggttccac
     7081 acacagcgcg gatgagtcag tgtttcatcg cgtttcgcat agccgttttc atcgaactga
     7141 tagttccagg acgatttgtc gtactggcgt ttttcagcgt cataaccgct gaacaggcca
     7201 tcttcgaaag caaaatcatc acgcaccagt aggctggcgt tggtgtaatg tttaacgtat
     7261 tcggcgttga ttttgttgtt ttcgatcagg tagcgcaaaa tgccagacag gaacgtaatg
     7321 tccgtaccgg aacgaatagg tgcgtaaata tccgccacag aagcggtacg cgtaaaacgg
     7381 ggatcgacaa cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa
     7441 ccgacgggat gcgcttcagc agcgttaccg cccattacca ttacgacgtt agcgtttttg
     7501 atatccaccc agtggttggt catagcaccg cgaccaaatg ttggagcaag acttgctacc
     7561 gttggtccgt gtcagacgcg cgcctggttg tctaccgcca gcatcccgag ggagcgggca
     7621 aatttctggg tcagcatccc ggtttcgttg ctggcaccgg aggcacacag cataccggta
     7681 gaaagccaac ggtttaccgt tacgccctgc tcgttctttt caataaagtt agcgtcacgg
     7741 tcagctttca tcagcttcgc aatacgggag aatgcttctt cccagctaat gcgctgccat
     7801 ttgtcagaac ctggcgcacg atatttcggg tagcgcagac ggttttcact gttgacgtaa
     7861 tccagcaaac cggccccttt cgggcacagc gcaccacggc ttaccggatg atccgggtca
     7921 ccttcaatgt gataaatcgc ttctctggcg ttttttgcgc catcacccag gctatacatc
     7981 aatagcccgc aacctacgga acagtatgtg caggtgttac ggatctcttt agcgcgtaat
     8041 aatttgtagt ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgccgctacc
     8101 gttgttccag ccataccgcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt
     8161 gctttcctct tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc
     8221 tttttgcgcg aaatcaaaaa gaacacgtgt ttgccacaaa ataatccgcc tgcgcaaatt
     8281 gacctacctc aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg
     8341 ctataaaatt cagcttaatt tttaacggca agagagacaa aacagcgagc atgacacgac
     8401 aaaaagcaac gctcataggg ctaatagcga tcgtcctgtg gagcacgatg gtaggattga
     8461 ttcgcggtgt cagcgagggg ctcggcccgg tcggcggcgc agctgctatc tattcattaa
     8521 gcgggctgct gttaatcttc acggttggat ttccgcgtat tcggcagatt ccgaaaggct
     8581 atttactcgc cgggagtctg ttattcgtca gctatgaaat ctatctggcg ctttccttag
     8641 ggtatgcggc gacccgtcat caggcgattg aagtgggtat ggtgaactat ctgtggccca
     8701 gcctgacaat tctctttgcc attctgttta atggtcagaa aaccaactgg ctgattgtac
     8761 caggattatt attagccctg gtcggcgtct gctgggtgtt aggcggtgag aatgggttac
     8821 attatgatga aatcatcaat aatatcacca ccagcccatt gagttatttc ctggcgttta
     8881 ttggtgcgtt tatctgggca gcctattgca cagtaacgaa taaatacgca cgcggattta
     8941 acggaattac cgtttttgtc ctgctaacgg gagcatgtct gtggatttac tattttctca
     9001 cgccacaacc agaaatggta tttagcacgc ccgtcatgat taaactcatc tctgcggcat
     9061 ttaccttagg atttgcttat actgcatgga atgtcggtat attgcatggc aatgtcacca
     9121 taatggcggt aggctcgtat tttacgcctg tactttcctc agcgcttgca gccgtgctgc
     9181 tcagcgcccc gctgtcgttc tcgttctggc aaggcgcact aatggtctgc ggcggttccc
     9241 tgctctgctg gctggcgaca cgtcgtggtt aaaataattg ccgggtctac ggcccggcag
     9301 ttaatcagat aaattccgac aatattaaaa aataaatttc aaataccagt aattcacctt
     9361 atatctcatt tcgacttcga tcacatatcg ataaataata ttattctatt tttctcattg
     9421 tttttaatca ccattctatt ttaaaaacag tatgtcattt ataaatgacg ccgtttaaaa
     9481 ttcgtttaga aaatacgttt aacgataatt aaaaggattc tgttaaatga aattaaaaat
     9541 agttgcggtg gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta
     9601 taacaaggat ggtaataaac tcgaccttta tggcaaggtt accgctctac gttattttac
     9661 tgatgataag cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac
     9721 gcaaatcaat gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa
     9781 cgatgaagcc aacggctcgc gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat
     9841 tagtgaattt ggctctctgg actatggtcg taactacggt gtcggctacg acattggttc
     9901 atggaccgat atgttgccag aatttggtgg cgatacctgg agtcagaaag atgtcttcat
     9961 gacataccgt accaccggtc tagcaaccta tcgcaactac gatttctttg gcttaattga
    10021 agggctgaac tttgccgcgc aatatcaagg caaaaataaa cgtactgaca acagtcatct
    10081 ttatggtgct gacgacacgc gtgccaacgg tgacggtttc ggtatctcct caacttatgt
    10141 ttatgatggc tttggtatcg gtgcggtata taccaaatcc gatcggacaa atgcgcagga
    10201 aagagccgca gctaatcctc tcaatgcctc cggtaagaat gcagaactat gggccacagg
    10261 tataaagtat gatgccaaca acatctactt tgcagctaat tacgatgaaa cattaaacat
    10321 gaccacctat ggggatggtt atatctctaa caaagcacaa agttttgaag tggtggcgca
    10381 atatcaattc gacttcggct tgcgcccatc actcgcttac ctgaaatcga aaggcagaga
    10441 tctgggccgc tacgccgacc aggacatgat tgagtatatc gacgttggtg cgacgtattt
    10501 cttcaacaaa aatatgtcga cctatgttga ttataaaatc aacctgattg atgaaagcga
    10561 ctttacccgt gccgtagata ttcgcaccga taacatcgtc gctacgggca ttacctatca
    10621 gttctaacgt cctctcctga ccagcccttc tctgggctgg tttttgattt aagtgctgct
    10681 gtatgactcc ttaagtaaaa atgagcaatt cttgatattt ctggtcattc acctcaatta
    10741 atattattcc atatcctccg gggacatctt actataatat ttattgatga gtgattatga
    10801 ccaatattaa tacagcttgt gtaaaaaata atgccagtta tcaacttaat aacgcattac
    10861 ctaacaaaga aaccatctcc agcaattttt gtgaacgact agaacaatgg ggtaataaat
    10921 cggttaataa tggtgaagaa agagcaattg ccgtggagcg aattaaagaa gcttacaatt
    10981 cgaacatggc atctcttgat ttgtcatatc ttgatttgag tgaattaccg cctatcccct
    11041 cgacagttaa tacgttaaat ctggaaaaca attgtctaac ttgtcttgac ttagctgaca
    11101 atgccagtct cgttaatatc aacctcagcg ttaataaaat taacacgata acctttccaa
    11161 aagaaccaaa actggaaaac atttatatta atcataacaa tctggaaagt ttagatttaa
    11221 aaaatcagca ttcattggtt aatctggaag cgcaaaataa tagcctaaaa aaatgatatt
    11281 tctgatagtt ataaactgaa atttcttaat cttgattata acaagctagc atcgctggat
    11341 gtctcccggc aagaatctct gattgagtta agtgcccatc acaatatgat cactgacctt
    11401 atattacaca atcatcccag gatgaaaaca atcactttaa acgacaacca tattgcacat
    11461 ttaaacgcca aaaacactac aaaactggaa tatttaaact taagcaataa caatttactg
    11521 ccaaccaatg acattgatca actaatatca tcaaaacatc tttggcatgt attagttaac
    11581 ggcatcaaca atgatccact tgcacaaatg cagtactgga ctgcagtaag aaatataatt
    11641 gatgacacta atgaagtgac cattgattta tcaggactta atttaaccac tcaaccacca
    11701 gggctgcaaa acttcacctc tatcaatctt gataataacc aactcacaca ttttgatgca
    11761 accaactacg atagactcgt aaagctaagt ctgaatagta atgctcttga gtcaataaat
    11821 tttcctcaag gcagaaatgt aagtattaca catatatcta tgaataataa tgctctcaga
    11881 aatattgata tagataggct ttcatcagtt acttatttta gtgcggcaca taatcaacta
    11941 gagtttgtgc aattagaatc ttgcgaatgg ctgcaatacc tgaatctcag ccataatcaa
    12001 ttaactgata ttgttgcagg taataaaaat gaactcttac tgctggatct atcccataat
    12061 aaactaacaa gtttacacaa tgtcttattt cccaacttga atacgttact tattaacaac
    12121 aatttgcttt cttaaattaa aatattctat agcaacttcc gcaatgttca gacattaaac
    12181 gctgctaaca accagttgaa atatataaat cttgatttcc tgacttatct tccatctatc
    12241 aaaagtttaa gactggacaa taataaaata acccacattg atactaataa tacatccgat
    12301 attggaactt tattccccat aataaaacag agcaaaaact taaatttttt aaatgtttct
    12361 gggaagaaca attgacctac tatgcagctc atgttattta atttgttttc cccagcactt
    12421 aagcttaata ctggcctggc aattctttcg cctggtgcat ttgaagttca ctctgacgga
    12481 atagatgcgg ataacgaatt gtttcactat actattaaaa aagcatatac cccatataat
    12541 atacacactt acaagacaga ggaagttgta aaccagagga atataaaagt taaaaacatg
    12601 acct
//
LOCUS       DAJTRH010000101        12424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTRH010000101 DAJTRH010000000
VERSION     DAJTRH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO339_003211"
     CDS             complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO339_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5927730.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1033..2124
                     /gene="rutA"
                     /locus_tag="OO339_003212"
     CDS             1033..2124
                     /gene="rutA"
                     /locus_tag="OO339_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5927731.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2124..2816
                     /gene="rutB"
                     /locus_tag="OO339_003213"
     CDS             2124..2816
                     /gene="rutB"
                     /locus_tag="OO339_003213"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5927732.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2828..3214
                     /gene="rutC"
                     /locus_tag="OO339_003214"
     CDS             2828..3214
                     /gene="rutC"
                     /locus_tag="OO339_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5927733.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3222..4022
                     /gene="rutD"
                     /locus_tag="OO339_003215"
     CDS             3222..4022
                     /gene="rutD"
                     /locus_tag="OO339_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5927734.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4032..4622
                     /locus_tag="OO339_003216"
     CDS             4032..4622
                     /locus_tag="OO339_003216"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5927735.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4633..5127
                     /gene="rutF"
                     /locus_tag="OO339_003217"
     CDS             4633..5127
                     /gene="rutF"
                     /locus_tag="OO339_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5927736.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5148..6476
                     /gene="rutG"
                     /locus_tag="OO339_003218"
     CDS             5148..6476
                     /gene="rutG"
                     /locus_tag="OO339_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5927737.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6558..6665)
                     /locus_tag="OO339_003219"
     CDS             complement(6558..6665)
                     /locus_tag="OO339_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927738.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7038..7634
                     /gene="wrbA"
                     /locus_tag="OO339_003220"
     CDS             7038..7634
                     /gene="wrbA"
                     /locus_tag="OO339_003220"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5927739.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7655..7882
                     /locus_tag="OO339_003221"
     CDS             7655..7882
                     /locus_tag="OO339_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5927740.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7920..9161)
                     /gene="agp"
                     /locus_tag="OO339_003222"
     CDS             complement(7920..9161)
                     /gene="agp"
                     /locus_tag="OO339_003222"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5927741.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9454..10710)
                     /locus_tag="OO339_003223"
     CDS             complement(9454..10710)
                     /locus_tag="OO339_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5927742.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10971..11891
                     /gene="cbpA"
                     /locus_tag="OO339_003224"
     CDS             10971..11891
                     /gene="cbpA"
                     /locus_tag="OO339_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5927743.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11891..12196
                     /gene="cbpM"
                     /locus_tag="OO339_003225"
     CDS             11891..12196
                     /gene="cbpM"
                     /locus_tag="OO339_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5927744.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12289..>12424)
                     /gene="torD"
                     /locus_tag="OO339_003226"
     CDS             complement(12289..>12424)
                     /gene="torD"
                     /locus_tag="OO339_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5927745.1"
                     /translation="KTLTALRQWLPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR
                     "
BASE COUNT         2969 a         3160 c         3135 g         3160 t
ORIGIN      
        1 tgaacgttcg ctgagtgtga ataccgctgc cgctggcggt aacgccagtt taatgacgat
       61 cggttaaaca ggtagccgga ggatgttaca acctcctccg tcatctttaa cgtggtcgaa
      121 tcccctcaat aataatccgc tgcacgtttt caaccgtttg attgaaaaat acctcatcgc
      181 gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg
      241 aagcccaaat cataaaaatc aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga
      301 cccaaccggc aatcagcgcc gatttctcat caattaatgc cttcaaatcg cccgtcagtt
      361 catccattaa caacggcgcg cctgccagca tctccataca gaacaggcgc gaagcctgcg
      421 gataatcgcg tgagacttcc agcttcagac ggatgtactc ttttatcgcc gccagcgggg
      481 cgaaatcttc acgaaacgct tttaacggtg ccagccagat atcgagaatc tgccgcagca
      541 cggcaatata cagcgcctct tttgacggga agtaatacag cagattggtt tttgaaacac
      601 ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt
      661 ccagtgctgc gctaagaatc gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg
      721 ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt gtagattggt aacaggatag
      781 caaaaccccc gcccggcctc gaccttctgc actctcatcg cgcacggatg catgttttat
      841 gtgcaactgt tttgaccgtt tagtccactt tttaccagat attttagata acacaatgct
      901 aacacattaa taatattcac tttttaaaaa ctggcatccg ctttgcgaac aagccaatac
      961 gcggctgaaa aggatatgca ggatgcagcg ccccgtctga ctttcacttt acgagacgaa
     1021 gagaggttga tgatgaaaat tggcgtattc gtacctattg gcaacaacgg ctggctcatt
     1081 tcgacccacg cgccgcagta catgccgacc tttgaactga ataaagccat tgcgcaaaaa
     1141 gcggagcatt accatttcga tttcgccctg tcgatgatca aactgcgtgg ctttggcggc
     1201 aaaactgagt tctgggatca caaccttgag tcgttcacct tgatggcggg tctggcagcc
     1261 gtgacctcgc gcattcagat ttacgccact gctgccacct taacgttacc tccggcaatc
     1321 gtcgcccgta tggccgcaac catcgactcc atctctggcg ggcgttttgg cgtcaacctc
     1381 gtgactggct ggcaaaagcc cgagtatgag cagatgggta tctggcctgg cgatgactat
     1441 ttctcccgtc gttacgacta tctcaccgaa tatgttcagg tgctgcgcga cctgtggggc
     1501 acggggaaaa gcgattttaa aggcgatttt ttcaccatga atgattgtcg cgtcagtccg
     1561 caaccgagta tccccatgaa agtgatctgc gccgggcaaa gcgacgctgg catggcgttc
     1621 tccgcccagt atgccgattt caacttctgt ttcggcaaag gcgtaaatac acccacggct
     1681 ttcgccccga ccgctgcgcg gatgaaacag gccacagagc aaaccggacg cgacgttggc
     1741 tcttatgtgt tgtttatggt gattgccgat gaaaccgacg atgccactcg cgccaaatgg
     1801 gaacactaca aagcgggcgc ggatgaagag gcgttaagct ggctaaccga acaaagtcag
     1861 aaagataccc gctccggtac tgacaccaac gttcgtcaga tggccgatcc cacttcggcg
     1921 gtaaacatca atatggggac gttagtcggt tcttacgcca gtgtcgcgcg catgttagat
     1981 gaagtcgcaa gcgtgcctgg tgcagaaggc gtgctgttga ccttcgatga ttttctgtcg
     2041 ggaatcgaaa ccttcggcga gcgcattcaa ccactgatgc agtgccgcgc ccatctctct
     2101 gcgctgactc aggaggtggc atgatgacaa ccttaaccgc tcgaccggaa gccattacct
     2161 tcgatccgca gcaaagtgcg cagatcgtgg tggatatgca aaacgcttat gccacgccag
     2221 gcggctactt agatctcgcc gggtttgatg tctcaaccac tcgcccggtc attgccaaca
     2281 ttcaaaccgc tgtgaccgca gcgcgagcgg cagggatgtt gatcatctgg tttcaaaatg
     2341 gctgggatga acagtatgtc gaggctggcg gccccggctc accgaatttt cataaatcga
     2401 acgccctgaa aaccatgcgt aagcagccgc agctgcaggg gaaattgctg gcgaaaggct
     2461 cctgggatta tcaactggtg gatgaactgg tgccgcagcc tggcgatatt gtgctgccga
     2521 agccgcgcta cagcggtttc ttcaatacgc cgctggacag cattttacgc agccgtggaa
     2581 tacgccatct ggttttcacc ggtatcgcta ccaacgtctg cgtcgaatcg acgctacgcg
     2641 atggcttttt tctggagcat ttcggcgtgg tgctggaaga cgcaacacac caggcggggc
     2701 cggaatttgc acagaaagcc gcgttgttca atatcgaaac cttttttggc tgggtcagcg
     2761 acgtcgaaac gttctgcgac gcgctttctc ccacgtcctt tgctcgtatc gcttaaggag
     2821 tttagcgatg ccaaaatccg taattattcc cgctggcagc agcgcaccgc tggccccctt
     2881 cgttcccggc acgctggctg atggcgtggt gtatgtctcc ggtacgctgg cttttgatca
     2941 acataataac gtgctgtttg ccgatgaccc aaaggcgcaa acccgccacg ttctggaaac
     3001 tatccgcaag gtgatcgaga cggcgggtgg cacgatggcg gatgtgacct tcaacagcat
     3061 ctttattacc gactggaaaa attacgccgc gattaacgaa atctacgccg agttttttcc
     3121 gggtgataaa ccggcgcgat tctacattca gtgcggactg gtaaaacctg acgcgctggt
     3181 ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc gatgaaactt tcactctcat
     3241 ctccccctta tgctgatgca cccgtagtgg tgttgatttc gggtcttggc ggtagcggca
     3301 gttactggtt accgcaactg gcggtgctgg agcaggagta tcaggtagtc tgttacgacc
     3361 agcgcggcac cggcaataat cccgacacac tggcagaaga ttacagtatc gcccagatgg
     3421 cagcggaact gcatcaggcg ctggtagccg cagggattga gcgttacgcg gtggtcggcc
     3481 atgcgctcgg tgcgctggtg gggatgcagc tggcgctgga ttatcccgcg tcggtaacta
     3541 tgctggtcag cgttaacggc tggctacgaa taaacgccca tacgcgccgc tgttttcagg
     3601 ttcgcgaacg attactgtat agcggcggcg cgcaagcatg ggtggaagcg cagccgttgt
     3661 tcctctatcc cgccgactgg atggcggccc gcgcacctcg cctggaggca gaagacgcgc
     3721 tggcactggc gcattttcag ggcaaaaata atttactacg tcgacttaac gccctcaaac
     3781 gcgctgactt tagtcaccat gcggatcgca tccgctgccc ggtgcaaatc atctgcgcca
     3841 gtgatgatct gctggtgcca acagcatgtt ccagtgaact tcatgccgcc ctgcccgata
     3901 gccagaaaat ggtgatgccc tatggcggac acgcctgcaa cgtgaccgat cccgaaacgt
     3961 ttaatgctct gttactcaac gggcttgcca gcctgttaca tcaccgtgaa gccgccctgt
     4021 aaggaattgc tatgaacgaa gccgttagcc caggtgcgct tagcaccctg ttcaccgatg
     4081 cccgcactca caacggctgg cgggagacac ccgtcagcga tgagacgtta caggagcttt
     4141 atgccctgat gaaatggggg ccgacatcag ctaactgttc tccggcacgg atcgtgttta
     4201 tccgcactgc agaaggaaaa gaacgtctgc gcccggcgct ttccagcggc aatctgcaaa
     4261 aaaccctgac cgcgcccgtc accgctatcg tcgcctggga cagtgaattt tatgaacggt
     4321 taccactact gtttccccac ggtgatgccc gcagttggtt tacctccagc ccacaacttg
     4381 ccgaagaaac agcgtttcgc aacagttcca tgcaggcggc ttatctgatc gtcgcttgcc
     4441 gtgcgctggg actggatacc ggcccgatgt cgggttttga ccgtcaacac gttgacgacg
     4501 ccttttttgc gggcagcacg ctgaagagca atctgctgat taatatcggc tatggcgata
     4561 gcagcaaact ttttgcgcgc ctgccacgtc tgtcctttga agaagcctgc gggctgttgt
     4621 aaggagcaaa ctatgaacat agttgatcaa caaacctttc gcgatgcgat gtcctgtatg
     4681 ggcgcggcgg tcaatatcat caccacggac ggtccagccg ggcgcgccgg gttcaccgcc
     4741 agcgccgtct gcagcgtgac cgacacgccg cccacattac tggtgtgcct gaatcgtggg
     4801 gcgtccgtct ggccggtatt caatgaaaat cgaacgctgt gtgtaaatac actgagcgcc
     4861 gggcaggagc cgctttcaaa tctttttggc ggcaaaacgc ccatggaaca ccgctttgcc
     4921 gccgcccgct ggcagacggg tgtgaccgga tgcccgcaac tggaagaggc tctggtttcg
     4981 tttgactgcc gtatcagcca ggtggtgagc gtcggcaccc acgacattct gttttgcgcc
     5041 atcgaagcga ttcatcgtca caccacaccc tacgggctgg tgtggttcga tcgcagttat
     5101 cacgcgctga tgcgccctgc ttgttaacct ccttaaggag acagctgatg gcaatgttcg
     5161 gttttcctca ctggcagtta aaatcgacct ctacagagag cggcgtggtc gcgccggatg
     5221 aacgattgcc gtttgctcag acggcagtaa tgggcgttca acacgcggtg gcgatgtttg
     5281 gcgcgacggt gttaatgccg attctgatgg ggctggatcc caatctttcc attttaatgt
     5341 cggggatcgg cacgttactg tttttcttta ttaccggcgg gcgcgtcccc agttatctcg
     5401 gctccagcgc ggcttttgtc ggcgtggtga ttgccgcgac gggctttaac ggtcagggca
     5461 ttaacccgaa tatcagcatc gcgctgggcg ggattatcgc ctgtgggctg gtttataccg
     5521 tcattggcct ggtggtgatg aaaattggta cacgctggat tgaacgacta atgccgccgg
     5581 tggtgacggg tgcggtggtg atggcgattg gcctgaactt agcgcctatc gcagtgaaaa
     5641 gtgtatcggc ttcggccttt gatagctgga tggcggtaat gacggtgctg tgtattgggc
     5701 tggtggcggt gtttacccgt ggcatgatcc agcggttatt gattctcgta ggattaattg
     5761 tcgcctgtct gctgtatggc gtgatgacca acgttttagg gctgggaaaa gccgtcgatt
     5821 ttactctcgt cagctatgct gcctggttcg gtctgccgca tttttccact cctggtttta
     5881 atggccaggc gatgatgttg attgcgcccg tggcggtgat tctggtggcg gaaaacttag
     5941 gtcatctcaa ggcggtcgcc gggatgaccg gacgcaatat ggacccgtat atggggcgag
     6001 cattcgtcgg tgatggtctg gcaacgatgc tttccggctc tgtcggcggt agcggcgtca
     6061 ccacctatgc tgaaaatatc ggtgtgatgg ccgtgacaaa agtctactcg acgctggtgt
     6121 ttgttgcagc tgccgtcatc gctatgctgt tgggcttttc gccgaaattt ggcgcgctga
     6181 tccataccat tccggccgcg gtgattggtg gtgcatcaat tgttgtgttc gggctgattg
     6241 ccgtcgcagg cgcaaggata tgggtacaaa accgtgtcga tttaagccag aacgctaatt
     6301 tgattatggt cgcagtaacc ttcgtgctgg gcgcgggtga ttttgctctc acgcttggcg
     6361 gttttacgtt gggagggatc ggtacagcaa cctttggcgc gattttactc aatgcgttat
     6421 taagcagaag gttggttgac gttccaccgc cagaagttgt tcatcaggag ccataaacag
     6481 aaaaccgccg ggttaccggc ggtgatgact ttttgacgag cttaagaaaa aaatgcattc
     6541 gtcattgatt agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta
     6601 cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc tcgatggttt
     6661 gccattactg acctccgtct gggtatagac atcatattgc atagaattaa cgcggcgtat
     6721 ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc ggacagaccc
     6781 gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct ccaataatta
     6841 tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt cattttgcga
     6901 caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga aatttgcact
     6961 ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat cgctacggaa tagagataac
     7021 acgaggagtg gttagaaatg gctaaagttc tggtgcttta ttattccatg tacggacata
     7081 ttgaaacgat ggcacgcgca gtcgctgagg gtgcaagcaa agtggatggc gcagaagttg
     7141 tcgttaagcg tgtaccggaa accatgccgc cgcaattatt tgaaaaagca ggcggtaaaa
     7201 cgcaaactgc accggttgca accccgcaag aactggccga ttacgacgcc attatttttg
     7261 gtacacctac ccgctttggc aacatgtccg gtcaaatgcg taccttcctc gaccagacgg
     7321 gcggcctgtg ggcttccggc gcactatacg gaaaactggc gagcgtcttt agttccaccg
     7381 gtactggcgg cggtcaggaa caaaccatca catccacctg gacgaccctt gcgcatcacg
     7441 gcatggtgat tgtccccatt ggctacgcag cgcaggaatt atttgacgtt tcacaggttc
     7501 gcggcggtac gccgtacggc gcaaccacca tcgcaggcgg tgacggctca cgccagccaa
     7561 gccaggaaga actgtctatt gctcgttatc aaggggaata tgtcgcaggt ctggcagtta
     7621 aacttaacgg ctaatcttca acaggaggat acgcatgcca actcaagaag cgaaagctca
     7681 tcacgtcggt gaatgggcat ctctgcgcaa tacgtcgccg gaaatagccg aggccatttt
     7741 cgaagtcgcc gggtacgacg aaaaaatggc agaaaagatt tgggaagaag gtagtgatga
     7801 agtgttagtc aaagcctttg ctaagaccga taaagattcg cttttttggg gcgaacagac
     7861 catcgaacgt aaaaacgttt aaccagcgac tcccccgctt ctcgcggggg agttttctgt
     7921 tatttcaccg cctcattcaa cacgctatca aacttatcca tcgggcagaa accgttggca
     7981 tctatcgggc aaccgcttaa ttccagcgtc acgcgctgcg caggtgcctg cagggttaac
     8041 gcatcggcat tacgtaactg ttccgcactc tggtacacat attcaatttt catcaaatcg
     8101 cgattggctt tgctgtcatg ccaacgctgg aaaacgattt tgccgccaat cggcgtgcgt
     8161 tcgttctggt catgcaactg atacggtttg aaatccagcg ccgttaacag agaggctatg
     8221 ttggagtcgt gcccaactaa caccgtgatt ttcggagcac tggcgcgatc ggtgaccagg
     8281 gctttgtcga tataactgac cagcggtttc gcaacattgc gcgccacttc cggtgaggta
     8341 aacagactgt cctggtaacc gtttttcagc ttcgacaata ccttccactg ctggtcagat
     8401 ttgatttctc cccaggccac ctgatccatc ggaaaacctt cgtagtattg caaagtaaac
     8461 gcatctacca gcgagttgcc gactttcagc ggcccggaaa cacctggttc ttgttgatac
     8521 ttggcgctaa aggtattttt gccatccacc agcgaacact gttgtttctc tttacaggca
     8581 ggggaatctt tatagttaac gattttttcc agtagctggt agctgtcggt aagctggagt
     8641 ttgctgagct ctttctccat tgccgccacc gcctgttcac tgaatgcggc ggaatcatcg
     8701 gtgatcatcg ggttaaaggt tgggtccata gtgcccattt tttcctggtg atgcacagga
     8761 atatcacacc cagggaatgc accagtaata aagaactggg cggtagcgac ggtacgttgc
     8821 agactattgg cataggcata aacggtgtcc ggcggcgggc attcccccga tttcactatc
     8881 ccctgctctg ccagccattc acgcatgtaa tggcccatat acacttcgag cacgccacct
     8941 ttggtggtga gttgcccacc ggggacgtcc cattcgggcc atttattcgg cgtcgactgc
     9001 tccagcacac tgccattgtt cgccagcggc gcacgtaagt tatggcggct catcatgagc
     9061 acttgctgta gctgatagcc ttccggtacg gtttgtgcct gagcatttga agcgagtaaa
     9121 actatccctg ccacagttgc ggcgattagc gttttgttca ttcctggcac ctcttttgtt
     9181 atcaatcatt aaagagtgtg acagaaatat gacttttttc cgggaacagg ttcgcaaaat
     9241 ggcttgttga acgcctgttt ttctatatac atcttcaaaa acaggcaagg tttgacgcaa
     9301 actatgcctg attagcccct gcctcgcgta cgccgctgat aattgcacct gtggctaaac
     9361 agaatatctg cggcgttacc aatacagaga caatatttac caataaaaaa gtgtgagact
     9421 aataacaatg gtttttagca tatgcctttg ccattaagtt gcataacgat ttaaatagtc
     9481 aagttgcgca ttcatttcct tattttttaa cttataagcc tgagaaaaaa gttcaggagg
     9541 gaggtaaaca tcataatgat tgccagtgtt tgttaaatat ccatactcaa gatcttcatc
     9601 tgttgaaaag gattcatttc ccaaggagat tgatgccaca ttactattaa caataaataa
     9661 tgagcaaatg ctatctgaaa gaacagcaag aaaattcttc accgctttaa tcctatccga
     9721 cgtgtcggta tctaaagcaa gcaaattctt gttatcatta aatataaact cgggtaaatg
     9781 cagtatgcat tttttgtcat tcaagcaact atcaatgaat tgttttttaa aaaacagaaa
     9841 gctccatgac ataaaactac ccacaccctg aattttgttg tcagattgca gatcaacgcc
     9901 aaataatgtc tttacatctt ccctgagatt atagaatgcc gtatcaacaa gagcctcgat
     9961 cgtttgttta tcaacaattt taataaactc ttcgtatact tgcttttgcg tcttatggct
    10021 gcccagcgcc catgaatact catgttcttt taaaaccagt atggctctta acagacaata
    10081 tccatcgcca tctactggag cgtattttaa tccatatgtt ttattaattt cactctgaat
    10141 aaaagatatg ccagacaaat caataccagt atctaaatca gatattcccg gcatatttat
    10201 ctgttctggc tggatactca tattttcatt atctaaacaa tactcagaaa aaacatcatc
    10261 acacgcatta atgttttcaa tatattccat aaagccatac caataataac ctttaaaatc
    10321 tgtgatagac tctgaacctc tgcccggcaa ggacactcta acaatatcaa catcttgaac
    10381 atattgtata gtatatgtaa tacctcctga aacccctgaa taaatttgtg tatcattagt
    10441 acgcaatgtt ctcataaata atgatagatc attatcattc tgcatttcct caaattcttt
    10501 ttctagtgat tcacattcca gacatggagt atcctcttca tttactttac gtctgagttc
    10561 attgtacaac cgcataaaac acaccagtgt gttttcaaaa agcgtgattg aatatgtttg
    10621 atttttttcg tactcatttt ttatatcgtt ccaggcctgt ttcaaataat tatttgcagt
    10681 gcaactaatt ccatgtatat tactacccat atatagcgtc tataaaattt aataaataat
    10741 gacgccctag ttaaacttaa agtgcctggt tcaactatca aaaatcgctc accctttttc
    10801 acctgtttaa aatatgttca gcaacccatc ttgatggcga cctcctctcc gcgatgattt
    10861 caataacata ttctgtgttg gcatatgaaa ttttgaggat taccctacac ttataggagt
    10921 taccttacag gggttccttc aatttgtgtt gatttacgcg agataacgct atggaattaa
    10981 aggattatta cgccatcatg ggcgtgaaac cgacggacga tctcaagaca atcaagaccg
    11041 cctatcgtcg actggcccgt aaataccatc ctgatgtcag caaagaaccg gatgccgaag
    11101 cccgcttcaa agaggtcgct gaagcctggg aagtgttaag tgatgaacaa cgtcgcgctg
    11161 agtatgatca gatgtggcaa catcgcaacg atccgcaatt taaccgtcag ttccatcatg
    11221 gcgacggtca gagttttaat gccgaagatt ttgacgatat cttctcgtca attttcggtc
    11281 agcatgcccg ccagagccgt caacgccccg ccgcacgtgg ccacgatatt gaaatcgaag
    11341 tggcggtatt cctcgaagaa acgcttactg agcataagcg taccatcagc tataacctgc
    11401 cggtttataa cgcctttggc atgatcgaac aggaaatccc gaaaacgctg aatgtgaaga
    11461 tcccggcagg cgtcggcaat ggtcaacgca tccgcctgaa aggccagggg acgccgggcg
    11521 aaaacggcgg tccaaatggc gatttgtggc tggtgattca tattgcgcca catccgctgt
    11581 tcgatattgt cggccaggat ctggaaattg tggtgccggt tagcccgtgg gaagcggcgc
    11641 tgggtactaa agtcaccgtt ccaacactga aagaaagcat tttgctgact atcccgccag
    11701 gcagccaggc cgggcaacga ttgcgcgtta aaggcaaagg tctggtgagc aaaaaacaga
    11761 ccggcgatct gtatgcggta ctgaaaatcg tgatgccgcc gaaaccggat gaaaacactg
    11821 ccgcgctgtg gcagcaactg gcagacgccc agtcgtcttt tgatccacgt aaagattggg
    11881 ggaaagcata atggctaatg ttacggtgac ttttactatt accgaatttt gcctgcatac
    11941 cggcatctct gaagaggagt tgaatgaaat tgtcggtttg ggggtggttg aaccgcgtga
    12001 gatccaggaa acaacctggg tatttgacga ccatgccgcc attgtggtgc aacgcgcggt
    12061 acgcctgcgt catgaactgg ctctggactg gccggggatc gcggtggcgc tgacgttaat
    12121 ggatgatatt gcgcacctga agcaggaaaa ccgcctgcta cgccagcggc tttcccggtt
    12181 tgtagctcat ccgtgatgtg tgtttgccgg atgtgatgac gcatccggca actgcgccat
    12241 aatgcctgat gcgacgctaa acgcgtcttt tcaggcgcat aaacgacgtt atctgttttg
    12301 gtggtcgcac tccactaaca ccagcaataa ctggcttagt gccgcgtaaa aaccaaagct
    12361 gtcatactgt cgacaacgcg cagcaaactc tggtaaccat tgccgcagcg ccgtcagtgt
    12421 tttt
//
LOCUS       DAJTRH010000102        12295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTRH010000102 DAJTRH010000000
VERSION     DAJTRH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..1035
                     /locus_tag="OO339_003227"
     CDS             <1..1035
                     /locus_tag="OO339_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5927746.1"
                     /translation="FPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHG
                     VWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTI
                     YDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFY
                     VIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAW
                     PHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKV
                     FNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIP
                     APIRPIARRT"
     gene            1036..3201
                     /locus_tag="OO339_003228"
     CDS             1036..3201
                     /locus_tag="OO339_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5927747.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3215..4126
                     /locus_tag="OO339_003229"
     CDS             3215..4126
                     /locus_tag="OO339_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5927748.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4126..5421
                     /locus_tag="OO339_003230"
     CDS             4126..5421
                     /locus_tag="OO339_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5927749.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWNFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5466..5696
                     /locus_tag="OO339_003231"
     CDS             5466..5696
                     /locus_tag="OO339_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927750.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5674..6174
                     /locus_tag="OO339_003232"
     CDS             5674..6174
                     /locus_tag="OO339_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5927751.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6174..7592
                     /locus_tag="OO339_003233"
     CDS             6174..7592
                     /locus_tag="OO339_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5927752.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7592..8440
                     /locus_tag="OO339_003234"
     CDS             7592..8440
                     /locus_tag="OO339_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927753.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8440..8895
                     /locus_tag="OO339_003235"
     CDS             8440..8895
                     /locus_tag="OO339_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5927754.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8898..9590
                     /locus_tag="OO339_003236"
     CDS             8898..9590
                     /locus_tag="OO339_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5927755.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9600..10931
                     /locus_tag="OO339_003237"
     CDS             9600..10931
                     /locus_tag="OO339_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5927756.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10932..>12295
                     /locus_tag="OO339_003238"
     CDS             10932..>12295
                     /locus_tag="OO339_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5927757.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPS"
BASE COUNT         3298 a         3057 c         3234 g         2706 t
ORIGIN      
        1 tttccgaacc ttgttgatca ccttctggtt aagcatcaca cggctgatgg cgttgaagat
       61 ggcatttcaa tctgctactt caagccatac tcgcaaggcc gtgctcgctg gcagggtgac
      121 acaatccacg gcgtgtggtt tgacgaagag ccaccataca gcatttatgg cgaaggtctt
      181 acccgtacca acaaatacgg gcaattctca attctgacgt ttaccccgct gatggggatg
      241 tctgacgttg ttaccaagtt cctgaagaac cccagcaagt cgcagaaagt ggtcaacatg
      301 accatctatg acgctgagca ctataccgac gagcagaaag agcaaatcat cgcatcatat
      361 cccgagcatg agagagaggc gcgtgctcgc ggtattccta cgatgggtag tggtcgaatc
      421 ttccagatac cggaagagac gattaagtgt cagccgttcg agtgtcctga tcacttctac
      481 gtaattggcg ggatggattt cggatgggat cacccacagg cgcaagttca gctttggtgg
      541 gataaggagg cagacacaat ctacgtttca cgcgtgtgga aggcgaaaga aaaaacagcc
      601 gttcaggcgt ggggagccgt taaatcatgg gcgcataaag tgccaaccgc atggcctcat
      661 gatggaaacc agcacgagaa gggcggcggt gagcagctca aagggcagta tgcagacgct
      721 ggatttatga tgttgcagga gcatgcgaca tggcctgatg gcggtaacgc tgttgagcca
      781 ggcatcactg aattgcgcga catgatgctc gatggtcgct tcaaagtatt caatacctgt
      841 gagccattct ttgaggagtt ccgcctctat caccgtgatg aaaacgggaa aatcgtcaag
      901 cttaacgacg acgtgctatc cgccgttcgc tatgcataca tgatgcgccg cttcgcaaaa
      961 atgatgcgcg acatcaaaaa accaaaagag aaaaagatac cagccccaat caggcccatc
     1021 gcacggagaa cttaaatggc cgacgaaaac agactcaatt ccattctgtg taagtttgac
     1081 gcagactgga tggcgagcga tgaagccaga accgaggcga caaatgacct gtattttagc
     1141 cgagtgtcgc aatgggatga ctggctatca aactacacta ccctgcaata tcgcggacaa
     1201 ttcgatgttg ttcgcccgga ggtcaggaaa ctggtcgcag agatgcgccg gaaccctgtc
     1261 gacgttctct tccgacccaa agacggcgct aatcctgatg cagccgatgt attgatgggg
     1321 atgtatcgta ctgatatgcg ccataacacg gcaaagattg ccgttaacgt tggcgttcgt
     1381 gagcagatag agtcaggcgt tggtgcatgg cgtctggtca cgcagtacga agacaacgat
     1441 ccaacaagca acaatcaggt aatccgacgc ctgccaatcc atgaagcctg ctcacacgtc
     1501 atatgggacg ccaacagcaa gcagatggat aagagcgacg ctaagcactg cacggtgatt
     1561 aacgccttgt cgcgcaatgg ctggaaagag ttcgcagagg attacggtat tgacccggat
     1621 accctgccat ctttccagaa tcctaacgat acatggctat tcccgtgggt gtcgaatgat
     1681 gtcgtctacg tcgctgagta ttacgaggta gaagaggaga aggagaaggt cttcatctac
     1741 cgcgacccgc tgacaggtga gccggtcagc tattaccagc aggatatcaa agacgtcatc
     1801 gacgacctgg ctaatcgtgg attcattaag gtagcagagc gcaaggtgaa gcgtcggcgt
     1861 gtgtataagt cgatcatcac ctgtacgcag atactgaaag accgcgagaa gatagccgga
     1921 gagcatattc caatcgttcc agtgtatggc gaatggtcat tcgctggtga caaggagtgc
     1981 tacgagggtg tggtaaggct gacgaaagac ggtcaacgcc ttcgtaacat gattatgtcg
     2041 ttcaacgccg atattgttgc tcggtcaccg aagaagaaac cgaccttctt ccctgagcaa
     2101 atcgaaggct acgaatacat gtacggcgga aatgatgact atccgtacta tctgcagaac
     2161 aggactgatg aaaacggtaa cgacctgccg attggtccaa tctcctacat ggaaaaccct
     2221 gaagtgccgc aagccaacgc ttacatgctt gaggctgcca ccaacgcagt gaaagaagtg
     2281 gctagtcttg gcgtggatgc gcaggcggca aatggtcagg tcgcttttga taccgtcaat
     2341 caactgaaca tgcgggcaga ccttgagaca tacgtgtttc aggataacct ggctaccgca
     2401 atgcgacgtg atggcgagat ttatgcctca atggtcaacg atatttatga cgttcctcgt
     2461 catgtaacgc tgacacttga agatggaagc gagaaagacg ttcaactcta tgcgcaagtt
     2521 gtcgattacc agtccggcaa tgtggtcaca ctcaacgaca ttcgcggtcg atatgagtgc
     2581 tatacagacg ttgggccatc cttccagagt atgaaggaac agaaccgcgc agagattcag
     2641 gagttgctca ccaaggttcc gcaaggtact ccagagttcc agatgctgat gctgcaatac
     2701 ttcacgctgc ttgacggtaa aggcgtcgag atgatgcgag agtacgcgaa caagcaactg
     2761 gtgatgatgg ggctgaagaa accagaaaca cctgaagaga tggagatggt gcagcaggca
     2821 caacaacagc cgcagcagcc atcagcagag caaattcagg cgcagggtat ccttctgcaa
     2881 ggtcaggctg aattgctcaa ggcagagaac caacaggcgc agattcaggt tgaagctgcc
     2941 aaggttgaag cccaaaacca actcaacgcc gcgaagattg cagaaatatt caacaatatg
     3001 gacctcgaca agcaggcaga actgcgtgag tacctcaggc tcgtaggtca attccagcaa
     3061 cagcgcagca aagatgctcg tgctaacgct gagctgcttc ttaaagatgc agaccagact
     3121 cattcacaac gcatggattt cgcgaatctt atgcgtcaag ttcaaatccc ctccggcgga
     3181 gtagccgaga cacctcaata agagagagtt aatcatggac caaaccaccg acattcaggc
     3241 ttctgaagaa ttaaccctgc ccggcaatca tgcagcggca tctgctgatg gcttagttgt
     3301 cgataatgcc aacgacagcg caggtcagga agaaggcttt gagattgtcc ggaaagacga
     3361 tgagaaacca aaacaagacc cggcaactaa tgctgaattt gcccgtcgcc gcatcgaacg
     3421 caaacgccag cgtgagcttg agcagcagat ggaagcggtt aagcgtggag agttgccgga
     3481 gcacctgcgg gtgaaccctg agttaccaaa acaaccagac cctaacgatt atctttccga
     3541 agatgcactg gctaagtacg actatgacca gagccgcgca ctggctgcct tccagcaggc
     3601 aaacagtgaa tggcagatca aggctatgga cgcacgaagc caggctgtcg ccgagcaggg
     3661 tcgcaaaact caggagttca cccagcaatc agcgcaatac gtcgaggcgg cccgtaagca
     3721 ctacgaggca gcggaaaagc tcaatatccc tgactatcag gagaaagagg atgcattcat
     3781 gcaactggtg ccgccagcag tcggtgccga catcatgcgc ctcttcccgg agaaatccgc
     3841 cgctctcatg tatcaccttg gtgctaatcc tgagaaaaca cgccagttgc tggcgatgga
     3901 cgggcaatcc gcgctgattg aactcactcg actgtcagaa cgtttaactc tcaagcctcg
     3961 agccaagcct gtttcagaag ccccgttacc tgatgaaccc attcagggac atgctgttgc
     4021 tgcaaatatc tctgcgattg aaaagcagat ggaggcggca gcaaacaaag gggatgtaga
     4081 gacgtaccgc aagctcaagg cgcaactgaa taaaggaatt cgataatggc attaaatgaa
     4141 ggtcaactgg tcacgtatgc tctggatgaa atcatcggaa ccgtccagaa cctgacgcca
     4201 atggcgtcaa aagtgacaaa atacccccct ccggcagaat ccatgcagcg ttcaagcaac
     4261 accgtgtgga tgcctgttga gcaggaagcg ccaacccaga ctggctggga tttaactggc
     4321 aacgcaaccg ggattctgga actctccgtg aagtgcaaca tgggcgatcc ggataacgat
     4381 ttcttcgagc ttcgtgcaga tgacctgcgt gatgagcgtt cttaccgtcg ccgcatccag
     4441 gcatccgcca aaaaactggc gaataacatt gagtcagcga ttgccaaaca ggcaactgaa
     4501 atgggctcgc ttgttgttca cgatacccgc gcaattggtc catcgactgg cctgcctggc
     4561 tggaattttg tgtctgatgc agagcgactg atgttctccc gtgagctaaa ccgcgacatg
     4621 ggcatcagtt acttcctgaa ccctgacgat taccgcaaag caggccgcaa cctggtagat
     4681 ggtgacatct tcgggcgcgt tcctgaagaa gcatatcgaa acggtactat tcagcgtcag
     4741 attgctggct ttgatgaaat tcttcgctca ccgaaacttc cggcagttac caagtcaacc
     4801 gcgactggtg taactgtttc tggtgcgcag aagtttaagc cgcaggcata cacccttgat
     4861 accgatggta acaaagagaa cgtcgacaac cgtgttgcaa cggtgaccgt atcctccacc
     4921 accggattta agcgcggcga caaaatcagc ttcattggtg tgaagttcct gtctcagatg
     4981 gcgaagaatg tgctgactga tgacgcgact ttctcaatca cccgtgtgat cgatggtact
     5041 cacatcgaaa tcacgccgaa gccgattgcg ctggatgacg cgtcactgac aaaagaagag
     5101 aaggcttacg ctaacgtaaa cacctctctt gctgatacca ctccggtaaa cgttctgaac
     5161 gtggcaacaa ccaccgctaa cgtgttctgg gctgatgact caatccgcct gctgtctcag
     5221 ccgatcccgg taacccatga actgtttgct ggcatgaaaa cgtcttcctt cagcattcca
     5281 ggtattggtg ttaacggcat cttcgcaacg cagggtgata tcaacactct gtctggtaag
     5341 tgccgtattg ctgtgtggta ttcagcatgt gctgtacgac cagaggcaat tggtgttggt
     5401 ctgcctaacc agaccgcgtg ataaccagag ggagcttcgg ctcccgtttt tatctggaga
     5461 caagcatgac acacatgatc tttcgtcatg gcgacatgaa gaagtggaaa ggcgttggct
     5521 acgactttga aatcgtgaaa gccgaagagc ttcaggaata tctggatgct ggctggtttt
     5581 cacatcctga tgaccttttg aaggatgttg cagagccaga gccagaagaa aaacagcgta
     5641 aaaagcctgg tcgaaaacct aaggcggcag cagatgaacc tgacaacgaa gggtgattta
     5701 gttcttgcgg cattacgtaa gctcggtgtt gcatcaaatg ccacgttaac cgatgtcgaa
     5761 ccgcagtcta tggaagacgg cgtcaacgac cttgaaatga tgatggctga atggcttggc
     5821 ggtgatgcgt cacctggtat caacgttggc tacatttttg ctgatgcaga tgttgctccg
     5881 gatccgggcg atgagcacgg tttatcaaat aacgctatca atgccgtcat tttcaacctt
     5941 gcctgccgaa ttgctccaga ttatgcgctg gaagcgtctg caaaacttat aaccactgcc
     6001 agatacggga aagagcgact cgtcaaactg tctgcaatgg acagggcaaa agcagctaaa
     6061 tgtaagtccg gttatccaaa ccgtatgcct gttggtagtg gtaaccagtt ggcgaagtgg
     6121 aatggttgga attacttcca ccgaaaggaa ccttgcgata acgggagcga ataatgccta
     6181 ttcagcaact tccgcttatg aaaggtgtcg gcaaagactt ccgaaacgcc gactatatcg
     6241 actatctgcc agtgaatatg ctggctacac ccaaagaaat cctgaacagc agcggatatc
     6301 ttcgctcatt cccgggcatt gccaaacgtt ctgacgtgaa cggtgtatct cgcggcgtcg
     6361 agtacaacat ggcgcagaat gctgtttatc gcgtgtgtgg cggcaagctg tacaaaggag
     6421 aaagtgaagt cggtgatgtt gccggaagtg gtcgtgtatc aatggcgcat ggtcggacat
     6481 cacaggcggt aggcgttaat ggtcaactgg tcgagtatcg ctatgatggc acggtcaaaa
     6541 ccgtctcaaa ctggcctaca gacagtggat tcactcagta tgagttaggt tcagttcgcg
     6601 acattacgcg cttacgtggg cgttatgcgt ggtcaaaaga cggaactgat tcatggttta
     6661 tcactgacct tgaagacgaa tctcatcctg accgttacag cgcacaatat cgtgccgagt
     6721 ctcagcctga cggcatcatc ggtatcggga catggcgaga cttcatcgtc tgctttggtt
     6781 catcgactat tgaatatttc tccctgactg gcgcaaccac cgttggtgcc gctttgtatg
     6841 ttgcacaacc atcgctgatg gtgcagaaag gcattgccgg aacttactgc aaaacgccat
     6901 tcgctgattc gtatgcgttt atcagcaatc cggcaacggg tgcgccgtct gtgtatatca
     6961 tcgactccgg tcaggtgtca ccaattgcca gcgcgagcat tgagaaaatc ctccgctcct
     7021 acactgctga tgaactggct gatggtgtga tggaatcgct gcgatttgat gctcatgagt
     7081 tgctgattat ccaccttccg cgtcacgtcc tcgtgtacga cgcatcttca atcgccaatg
     7141 gcccacaatg gtgtgtgctg aaaacaggtc tgtatgacga tgtgtaccgc gctatcgact
     7201 tcgtttacga aggcaatcag ataacgtgcg gcgataagct ggaatctgtt accgggaaac
     7261 tgcaattcga tatcagcagc cagtacgaca agcaacagga acacctgctg tttactccac
     7321 tcttcaaagc ggataacgcc agagtgttcg accttgaggt tgaatcgtca actggcgttg
     7381 cgcagtacgc cgaccgcctt tttctctctg caaccactga ctgcatcaat tacgggcgtg
     7441 agcagatgat tgagcagaat gaaccgttcg tttacgacaa gcgtgttttg tggaagcgag
     7501 tagggcgcat caggaaaaat gtcggcttca aattgcgcgt tatcacgaag tcacctgtca
     7561 ctctgtctgg cgctcagata aggattgagt aatggctgat tcgaatctca atgtgccggt
     7621 aatcatccaa gctacgcggc tcgatacatc agtccttcca cgcaatatct tctcgcagtc
     7681 gtatctgctt tacgttatcg cacagggcac tgatgttggt aacgtggcta acaaagccaa
     7741 cgaggccgga cagggcgctt atgatgcaca ggtcaggaac gatgagcagg atgtgattct
     7801 cgctgaccat gagcagcgaa tttctgctgc ggaagcaacg cttgttaatc atgaggagcg
     7861 aatcagacag gcagaatcaa ctcttcagga ccatgaaaca cgaatagctc agaatgaaag
     7921 cgatatttcg tcgcttgaca caagagttca gtcgctggag tcgcaggttt cagaccatga
     7981 aacgcgcatc gatgctctgg agtatgcaac cacacgcaag aagtcagagg ttgtttactc
     8041 tggtgtatct gtaaccatcc cgacagcgcc gaccaacctt gttagcctgc tgaaaacgct
     8101 cacaccgtca tccggctcgt tggcaccatt cttcgacacc gttaacaaca agatggttgt
     8161 gttcaacgag aacaaaaccc tgctcttcaa gttgtcgatt gtcggcacgt ggcccagcgg
     8221 aaccgccaac aggtcaatgc agctaacctt ttccggctct gttcctgaca cactggtcag
     8281 cagtcgtaat gcggcgacaa caaccgacaa catcctgtta gctacgttct tcagcgtgga
     8341 taaagacggc tttcttgcca caaatggcag tacgttaacc attcagtcga atggtgcggc
     8401 gtttactgcc acaaccatca agataatcgc ggagcagtga tgattacatt caaaccaacg
     8461 cgaaacatcg acctgataga agcagtcgga aatcaccctg acattatcgc cgggagcaac
     8521 aacggtgatg gatacgacta caaacctgaa tgccgttact ttgaggtgaa cgtgcacggt
     8581 cagtttggcg gcattgttta ctatcaggag attcagccgc tgacattaga ttgccacgcc
     8641 atgtacctgc cagagattcg cggcttcagc aaggaaattg ggctggcgtt ctggcgatac
     8701 attctgacta acaccaccgt tcagtgcgta acatcgttcg ctgcacgcaa attccgacac
     8761 ggtcagatgt actgcgcaat gattggcctt aagcgtgtag gaaccatcaa gaaatacttc
     8821 aaaggcgtgg atgacgtgac tttttacagc gccacacgcg aagaactaat cgacttcctg
     8881 aatcacggga gatagccatg ttatatgcat ttaagctggg cagaaaactg cgcggcgagg
     8941 aaccttattg ccctgaaaaa ggcgggaaag gtggcagttc tgataaaagc gcaaagtatg
     9001 cagcagaagc ccagaagtat gctgcagacc tgcaaaatca gcagtggcag acgatcatga
     9061 aaaaccttgc tccgttcacg cctcttgcgg agcagtatgt taaccagctt cagaaccttt
     9121 ccagtttaga aggtcagggg caggcactta atcagtatta caactctaag cagtataaag
     9181 accttgcagg gcaggcgcgt taccagagtc ttgctgctgc ggaggcgacg ggtggacttg
     9241 gttcgacagc cacaagcaat caactggcta cgatcgcgcc gacactcggt cagtcttggt
     9301 tatcaaatca gatgagcaat tacaacaatc tggcaaacgt tgggcttggt gcgctgcaag
     9361 gtcaggcaaa cgccgggcag acgtacgcca acaacatgag cagcattgca cagcaaagcg
     9421 cagctcttgc cgctgctaat gccaataaac catcaagtct tcagactgca attagcggtg
     9481 gcacgtctgg tgcgattgcc ggtgcaggtc ttgccagcct tttgggaaca tcaacgcctt
     9541 ggggcgctgg tattggtgct ggtatcggat tgcttggctc gttgttttaa gggggaatca
     9601 tggctacttg gcaaggaaca aacggcggat tgttggctgg tatcggcggc gtcaactcaa
     9661 acgctccgag cgtaaatgac atcggcaata cgcttcagct tatcaggcag aacaatgata
     9721 ttgagcgttc aggcgctaac aatgttgggc tgactgcttt gcaaggtctt tcaggtattg
     9781 cgggtgtttt tcagcaggaa aagcaggctc agcggcagaa agaatttcag caggcgtacg
     9841 ctaatgctta tgcgtctggt gatcgcggtg ctttgcgtca gttggctact caatatccag
     9901 accagattga atccgttcgt aaaggcatgg gattcattga tgaagagcag cgtaattcta
     9961 tcggcacctt agcggctggc gcacgccttg cgtcatcgtc tccagaagca atgcaatcat
    10021 ggctgcaaaa caacgccggt gagttagctc gtgttggcgt taatcctcat gacgtcgctc
    10081 agatgtacca acagaacccg cggcagttcg gcgaatttgt cgatcacctg gggatgaaca
    10141 gtctcgggcc cgaaaaatac tttgacctac aggataaaat gcagggtcgc caggttacca
    10201 tgcgcggtca ggatctggat tcgcaaaccg ccgctcggaa tcaggcaatc acaatgcgcg
    10261 gacaagatat ccaggcgaat ttaggtcagc agcgcattaa tctggacgca gaaacaaacc
    10321 gcattaacaa cgaaaataag cgccttgacc ggatgctatc agcagaaact aacgacctga
    10381 agcgccagga aatacagagc cgcatagcag ccaacaacca gcagttgcag cagaagcagc
    10441 aagcgctaaa tgatggctac aaagacggca tcaacaccct cacaaccagc atgttcactc
    10501 tgaacgatat cgttagttct ccttcactta agagcattac aggcttacgt ggagtaatcc
    10561 ccaacgttcc aggctcacag gctgcagaca ctcaggcacg acttgatacc tttaaatcac
    10621 aagcatacct gacagcggtt caggccatgc gaggcatggg cgcactttct gatgccgagg
    10681 gcaaaaagct cgaccaggct gttggttcgc tgcagaactc gcagagcgag gagtcctttc
    10741 gtcgcaacgc tggcgtcatc ctgaacacgc tcaaccagaa gcgtaatgag gcggttggta
    10801 agtacgttca gcaaaacggt atcaagcgag tggaagcgcc tcaggcttct atagattacc
    10861 tgaagcagca ccccgagctg tcaatcgact tcattaatcg ctacggatat cttccatctt
    10921 tggggcagta aatggctaat taccgtgatt tgttagagca ggctggcgca cgttacggtg
    10981 tgccagaagg gttgatgact gcactgggtg ccaaggagtc ttcttacaac cctgccgcag
    11041 taagctccgc cggggctgta ggattgactc aggtcatgcc tgggacatgg cgtgatatgg
    11101 gttataccga tgagcaaatg caaaaccccg aatatcaggc tgacgctggc gcgcgctatc
    11161 tggcaaagat gtaccagcag tttggtaact ggcgtgactc tcttcaggct tatcacgacg
    11221 gtcccggcaa cgttatgaag gcaaagcgtg gtgaatatac gccaggacct gaaggccgcg
    11281 gttacgttga tgatcgcttt gctcaatggg ctggtgaccc ggtgacagac tcaacagtcg
    11341 aacagcgcgc cacctctgca aaggtacatc ctcagcaaga ccctaacaac ccgtttgcac
    11401 aactggaagt acagtcatcc gaacaagtat cggcatcagg tgtgcagtca gacccaaata
    11461 atccatttgc tcagattgag cagcaggcag ccagtcagca gccacctcaa cccgtaagtt
    11521 ctgtcgcacc gaaacctgtt cagcaacaaa cagttaatca ggccaataat gcaccaacac
    11581 gggaagaacc atcattgatg caacaagctg gcgattggct cactggtggt caaagtgcag
    11641 ggcaaattgc agaacaggct ggtcgtggtc tggtaaacat accatttgac gtattgcagg
    11701 gtggcgcaag tctgattaat gcaatcagtc aggggcttgg ggggccaaaa gtattggatg
    11761 atgtttatcg tccagtagac agaccgacag acccctacgc gcaagctgga gagtcaatag
    11821 gcggttatct tgttcctgga gcaggtgtag ctggaaacat ggtcattggt tctctcgctg
    11881 acgcggcgaa tcaacggggt gattttgccg aaaatgccgc tattaatgcc ggacttagca
    11941 ttgctacgca tggcctgata aatggcgtta cccgtggtgt tcgtggtgca tcaaatataa
    12001 ttagtggcaa taaaacatct gcacagagag cgaccactgc gccaacagaa acatcaccat
    12061 tctccggtga tgccgctgca gcaacaaatc ctgcggttca tgccgcagag gcaagagtag
    12121 cacaaggtgt accaatgacg cctgcgacga agaacccaga ggaagtcgtt cgcacagtag
    12181 cagcacaaaa aaggccaaat ctcgcttcat cgcttgatga actagatatc aatcctcagg
    12241 ctgaagttct ggagtctgct gaaaggctta atgttgattc attactccct tcaca
//
LOCUS       DAJTRH010000103        12174 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTRH010000103 DAJTRH010000000
VERSION     DAJTRH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..592)
                     /locus_tag="OO339_003239"
     CDS             complement(<1..592)
                     /locus_tag="OO339_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5927758.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLN"
     gene            1112..2011
                     /locus_tag="OO339_003240"
     CDS             1112..2011
                     /locus_tag="OO339_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5927759.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            2060..3073
                     /gene="yhjD"
                     /locus_tag="OO339_003241"
     CDS             2060..3073
                     /gene="yhjD"
                     /locus_tag="OO339_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5927760.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3484..4806
                     /locus_tag="OO339_003242"
     CDS             3484..4806
                     /locus_tag="OO339_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5927761.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4988..7048)
                     /locus_tag="OO339_003243"
     CDS             complement(4988..7048)
                     /locus_tag="OO339_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5927762.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7118..7885)
                     /gene="pdeH"
                     /locus_tag="OO339_003244"
     CDS             complement(7118..7885)
                     /gene="pdeH"
                     /locus_tag="OO339_003244"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5927763.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            8117..9046
                     /gene="kdgK"
                     /locus_tag="OO339_003245"
     CDS             8117..9046
                     /gene="kdgK"
                     /locus_tag="OO339_003245"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5927764.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9142..10638)
                     /locus_tag="OO339_003246"
     CDS             complement(9142..10638)
                     /locus_tag="OO339_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5927765.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10859..12145)
                     /gene="dctA"
                     /locus_tag="OO339_003247"
     CDS             complement(10859..12145)
                     /gene="dctA"
                     /locus_tag="OO339_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5927766.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
BASE COUNT         2916 a         3309 c         2995 g         2954 t
ORIGIN      
        1 tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga tacagggatt
       61 ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc tccttatttg
      121 attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac gatttgagat
      181 ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc atccccatcg
      241 ccgcactatt taccgccaac gcaaatgtct cgacggttga atcgcgaggc acaatggcca
      301 gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc gccgtgatta
      361 acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag caaaactcac
      421 catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc ttttgccata
      481 actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc tgtaaactga
      541 tttttattcc atgaataaat attgactgcc tgtcaaacat gactatttgc ataactgaat
      601 ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg cgagaaaaaa
      661 taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa aatagttacc
      721 gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta tttaaaacaa
      781 ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg aaattccgcg
      841 actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata gccaatacgt
      901 cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg gattcacctt
      961 tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt catgacaaca
     1021 ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat gcgcgttact
     1081 gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa tgcagttgtt
     1141 catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg ctttgccaaa
     1201 gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc agcttctcca
     1261 gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc aacgggcaaa
     1321 agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg ccaccagtat
     1381 cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt tactgccgcg
     1441 tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta gccataaccg
     1501 tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg cgttaccgga
     1561 agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg ccagtccgca
     1621 ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac acgcaatagt
     1681 gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca gcgttaatgg
     1741 cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa actatctcgc
     1801 cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc gcgaagccct
     1861 gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc tctccctttc
     1921 gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta tgcagtggct
     1981 ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga atgaccacga
     2041 cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca cccaggatct
     2101 ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag ttagccaggc
     2161 gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta ttgcgcacct
     2221 tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg cggctatcac
     2281 ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg cagggggctt
     2341 tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc tgcaaaacat
     2401 cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg ttcagcagcg
     2461 tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca actggatggg
     2521 taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct cgccgcaaga
     2581 tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg gtttgttgat
     2641 tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc aaatgattat
     2701 tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat tgattggcct
     2761 ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc ggttgccgcg
     2821 ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta ttggttttga
     2881 agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc cctctggcgc
     2941 agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc gtttgacgct
     3001 gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa tgccagggaa
     3061 aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat acgattttat
     3121 tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg ctataatgcg
     3181 cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg ggagcaaaca
     3241 atttcactcc aactcataac cccagcatat aaatccagtt ggtaactttt atttaacctg
     3301 aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa gaaaaatcac
     3361 tggcctaaac attatcccct ttttgcctgg tttttgacca tttccgcgat ttgttacaca
     3421 ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa ataagaaaag
     3481 attatgcaag caacagccac aacactcgac cacgaacaag aatacacgcc gatcaactcg
     3541 cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttctt cgacttttac
     3601 atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca gggcgatcca
     3661 acggcagcaa cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc gcgccccatt
     3721 ggctctgccg tttttggtca ttttggcgat cgcgttgggc gtaaagcgac gctggtcgcc
     3781 tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg ctatgccacg
     3841 attggtattt tcgccccact gctgctggcg ctggctcgat ttggtcaggg tctgggctta
     3901 ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc gcgcaaacgt
     3961 gcactgtatg gctccttccc acagctaggc gcaccgattg gcttcttctt tgccaacggc
     4021 actttcttgc tgctttcctg gctactgacc gatgagcagt ttatgagctg gggctggcgt
     4081 gtgccattta tcttctcggc ggtgctggtc attatcggcc tgtatgttcg cgtgtcgctg
     4141 catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa gatcccgctg
     4201 ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat tatgctggca
     4261 acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac cgccgccgcg
     4321 ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat ggcagttatt
     4381 ggttttggcg tgatggtgcc agtcgccgga ttactggctg atgcctttgg tcgccgtaaa
     4441 agcatggtga tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt taacccgctg
     4501 ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt aagtctgatg
     4561 gggctgacct tcgggccgat gggtgcgctg ttaccagagc tgtttccgac agaagtgcgt
     4621 tacaccggag catcgttctc ttacaacgta tcgtcgattc tcggggcttc cgttgcgccg
     4681 tatatcgcag cctggttgca ggctaactac gggttaggtg cggtggggtt atatctggcg
     4741 gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg acatcagtcg
     4801 ttgtaataag tgtgaaaagc ctgatgcgct acgcttatca ggactgaatt tccatacaat
     4861 atattgaatt cacaaggatt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
     4921 caaagcacac tttgccaaaa atctaaatct gtttaattgc cggatgtcag acatccggca
     4981 attatcatca cttcttcatc tgcgacaata tcgtccgaca ctgattagcc tccccttcgg
     5041 aaggtgagat cagtgccagt aacgccgccg ccggtgttac cagcgttgcc agtgccgcag
     5101 caacagcacc acgggcaatc agcggtccgg ctttcacccc agcctgcgga tttttaaacg
     5161 tcccacgcac atacagcggc gaacgcagtg tgataatccg aatcccttta ctctccggat
     5221 caatagtcaa atccagctgt tccgaagcaa aacttgccgt gccggtaaca ttaatcaacg
     5281 cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac gccgttggca atattcagat
     5341 tcgccgccgc gcagttcacc cgcacctcat cgtcaccaaa tatcgcaccg acaatgtagt
     5401 tgccgacatt cagcccaaca atctccatca ggttgcggct caccagcccg tcattcatca
     5461 acagtttcag gttgccgtta ctgttgccta aaagcgccgc caccgagtta ccgctaccgc
     5521 gtagttccgc gtcaccgttc atttccccca gcgtcttctg catcagttcc acatcgggca
     5581 tcagttcttt cagtttcagt cgacgagcct gaatatctgc ccgcccctgc atcggctttt
     5641 tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca aatttcagcg
     5701 gttgcaggcg caggtcagca tttttgagga tgatatgagt agaaagatcg ctaatcggca
     5761 ggctactgcc atgctcaatg cgccgccctt tgaagcgaac atcggcatcc ataacgtccc
     5821 atttatcggt ttcgaagcgg tcataaggca gcactttgcc cgcaggctga acgctttttt
     5881 cgcccttctt ctgttcagac cgtttcgact tttctgcccc tttcccggaa tcaacgccaa
     5941 tcaacggtcc caggtccgcc agccgcaatt gccgcgactc gacatcacct tccagttttg
     6001 gtcgtggctt tccggtggtg tagaccagag aaccgtggat atcgctatcg ccaattcgcc
     6061 cattaaaacc gcgataatca aagaccgacg atttttcagt gtcgattttc gctaccagcc
     6121 gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt tcatagagat
     6181 cacccagtga atcgccagaa aatttaagcc gtaaatcgac accgcccatc ttcattgggt
     6241 cattcacgac gccatcaaaa gcaacacggg tattacctga acggaaatca gcctgtaccg
     6301 gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc tatttttccc gtacccgtga
     6361 gcggttcacc gttataacgt ccctgcgcct tcaggccaaa aatgtaatcg cccacctttt
     6421 ctttatccgc tttacctttc gatccagtaa cttcgctgaa tggcagcggc ttgcctaacg
     6481 gatcaacaaa aatttccaga tccgctttgc ttactttgtc atcaatggcg atccgccctt
     6541 gatcgaaaag aatattatcc agccgaaacg accatgccga cggctttgca ttcgcgtctt
     6601 tgttgtcatc gttggcaaga ttaaacgtcc agttattgtt cttttcagag aggcgaatca
     6661 ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca gaccgttttg gtcagcagcg
     6721 ccagcggtgc cagcgttgcc tctactcgcg gcaaatgcac catcgtgact tcgggaatat
     6781 ccggtggatt accaagaatg atgtcttctg catgtacatg aggccacggc acccagctgc
     6841 gccagccagt ttcttgtttt tgccgctccc acaccacgcc cagattgcca cggatagcga
     6901 acggacgatt caactccgca gagactttct ggttgatggt cggtttgagt cgattccagt
     6961 caaaagttgc aatcaaaatg atcgccacga caatcaacaa caagaaagcc cctgaaatcg
     7021 cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct catattcttc ctgtgccagt
     7081 cctaaagata gtccagcccg gcggaaaatg aggcagctta tagcgccaga accgccgtat
     7141 tcagcgtttc catcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg aatgcgggcg
     7201 agttctgaac atcacgccac tcttccggtg tttctacgcc ctcgacaatc accccgcgac
     7261 aatagcgatt cattagatgt aaaagctgag aaaagagtgt gcggccttcc ggcgactgac
     7321 gcagcatcac aaacagttct cgcgcgattt tgatgtagtc ataacgcact tcacttagtg
     7381 cagagaaatt tgccatcccg gtaccaaaat catccagcca cagcgggcca aattcacaca
     7441 tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc caccagttcg aaacgcagcc
     7501 agggaagacg ctcaatctgg cgcaggattt ttggttgctg acgcagggcg atgagcgtag
     7561 ggccatcaat attgaccgat gccagcaggc cgtgctctac aaagaagtcg gctttttgcg
     7621 ccagcaaatc aatctgctct ttcacaacct ccatccgatg gctgacggtg atttcagtaa
     7681 aatagcgatc cggcggcagg cgttgcgaag ggttcaaggg atgcgtgacc accgttaata
     7741 gctccacggc cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg taagcacgct
     7801 cacactgcaa ccaaaaacgc cgttcctgca agctctcgat gcttgcttca gggttgctta
     7861 ttcgctggat aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg atgctgcccg
     7921 gactcgacaa agattatcgg cgcgtaaggg cagaacttta ccgcctggcc ggggtaaatt
     7981 tcctcactcc acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa taatggaaca
     8041 ctgttttaat atggttgacc agcaaaccac cacagcgcaa actaacgcta attttttaca
     8101 gatcaggttc acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca tgattgagct
     8161 ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc gataccctga acacttccgt
     8221 ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa cggcgctggg
     8281 aacggacagt tttagccagc agatgctgga cgcctggcac ggcgagaacg ttgatacttc
     8341 cctgacccaa cggatggaaa accgtcttcc gggcctttac tacattgaaa ccgacagcac
     8401 cggcgaacgt acgttctact actggcgtaa cgaagccgcc gccaaattct ggctggcgag
     8461 cgagcagtct gcggcgattt gcgaagagct ggcgaatttc gattatctct acctgagcgg
     8521 gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc tgctgcgcga
     8581 atgccgcgcc aaaggcggaa aagtgatttt cgacaataac tatcgtccgc gcctgtgggc
     8641 cagcaaagaa gagacacagc aggtgtacca gcaaatgctg gaatgcacgg atatcgcctt
     8701 cctgacgctg gacgacgaag acgcgctgtg gggtcaacag ccggtggaag acgtcattgc
     8761 gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg attcttgcct
     8821 ggtgtccatt gctggtgaag cgttagttga tgttccggcg gtgaaactgc cgaaagaaaa
     8881 agtgatcgat accaccgcag ctggcgactc tttcagcgcc ggttatctgg cggtacgttt
     8941 gacgggcggc agtgcggaaa acgcggcgaa acgcgggcac ctgaccgcaa gtaccgttat
     9001 tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc agcatatgca
     9061 aaaggctgga taaagtgtta cccctctatc cagctcatcc ataaatacct gatggttgtg
     9121 ctaactcatc accatcaggc attactgtgc aggtggaata tccgtcactt caggatgtac
     9181 gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat ggggccatga tttgatccca
     9241 ggccgcctgc aacgctttca tgttaaattc cggctcgcct ttcggctgca gcagtattaa
     9301 cgccatatca ttcgacaact gctgacgcag atcctgattt aacatctcca cagtcaggct
     9361 gttaaggaaa tcctgccgca gtttctgata ctgctccggc gcgatatcga cgacctgatt
     9421 ttgcaacgaa cgcatccgct ggcccatcag aatatcggta tcagcccgtg cataggcggc
     9481 aaacagtttc tgcagctcca gtttcttttg cgccactaac gcattgaact cttcttccgg
     9541 cagaccttta tcgcgaacct tcgccagttc acgcgccacc agattaaggt tgctgttcag
     9601 cttgtcgttt ggtgattcga tgttgatggc acactgcgca cgcagataca gcacacggca
     9661 gtcaaatcca agaccgatgt ctttgctgtc actggcgctt aacgcttgct gaacatgcca
     9721 gaacagcgcc tcgcgggcca ggtccgcacg ccagtagcgc agcagtgcgg ctgattcacg
     9781 aatcggctgc cacggcgtat cccacatgat agataaccgg tcctgacgca ccgcgtcagt
     9841 cataatactc accgcttccg cacgcagcgg agaaagcgtc ggcaccggag ctggcgtttc
     9901 acgtttgcct ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg agcgcgcatc
     9961 cacgtttccc accaccagta gcgtcattgc atccggggtg taccatttct gatagaaatc
    10021 tttaattttt tccgcttcaa cgggttgttt cagcggatcg gcaggatcgt gacctaacaa
    10081 ggttgaccct ttcagacgat agcgccacca gccctcttta gtatcggcag gccaggttgc
    10141 caccatgtcc tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg tcaatttgcc
    10201 agtggcattt gccagataag agagcgcttc tttcagcaaa tcgttacggt tattgggcaa
    10261 actcagatta aacagcgtgg tgtcataaga gacaattacc ggcggcatcg ggcgtttagg
    10321 gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac cgctttgcgt
    10381 tagcgcaata cgagggatgg cgtgactgta accgctctgt tgtgtacttt cggcgagcga
    10441 accggtatta accagcaggc gaatttcaac acgatcgctg ggacgctggg gggtggtcag
    10501 cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc catgctggat caggctggag
    10561 cgcatctgcc tgcacatagc cagcagtggc catcatcagc aaaccgcccg ctaaaagtcg
    10621 aatttttatg ccctgcatgt gaacccctga tcaactatcc tggtaataaa aaaactgccc
    10681 gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa gaacatgaaa
    10741 atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc aggcacaggg
    10801 gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag tggtgagatt
    10861 aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca tcgtccagtt
    10921 ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg ccgttaccga
    10981 ccaggttagt tagcgcacga gcttctgaca taaagcggtc gataccgagg atcagcgcca
    11041 gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg ataaagccac
    11101 tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac agcgtgattt
    11161 ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc gccattgtca
    11221 ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc agccccacca
    11281 ccgatttacg gcagccgagt ttctccatct tgtcgagcat acgcggcagc gccgactcgg
    11341 aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata aatttgaaga
    11401 tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac aggatacagg
    11461 taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg ccgtatttac
    11521 cgatggtaaa cgccattgcc ccgagcgcac caataggtgc cagacgcatg atcatattga
    11581 tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt tggcctttgc
    11641 tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc tgcagaatgt
    11701 taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg aaggcgacaa
    11761 tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca agcgttgccg
    11821 gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga ccaataatca
    11881 gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta cgaccgaccg
    11941 ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg acaggagcga
    12001 tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc tcgcctattt
    12061 caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc tgaaagtaaa
    12121 ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag gtct
//
LOCUS       DAJTRH010000104        12162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTRH010000104 DAJTRH010000000
VERSION     DAJTRH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..435)
                     /locus_tag="OO339_003248"
     CDS             complement(<1..435)
                     /locus_tag="OO339_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5927767.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFD"
     gene            complement(466..1158)
                     /gene="fimC"
                     /locus_tag="OO339_003249"
     CDS             complement(466..1158)
                     /gene="fimC"
                     /locus_tag="OO339_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5927768.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1378..1920)
                     /gene="fimA"
                     /locus_tag="OO339_003250"
     CDS             complement(1378..1920)
                     /gene="fimA"
                     /locus_tag="OO339_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5927769.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2401..3267
                     /gene="folD"
                     /locus_tag="OO339_003251"
     CDS             2401..3267
                     /gene="folD"
                     /locus_tag="OO339_003251"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5927770.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3269..3481
                     /gene="ybcJ"
                     /locus_tag="OO339_003252"
     CDS             3269..3481
                     /gene="ybcJ"
                     /locus_tag="OO339_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5927771.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3589..4098
                     /locus_tag="OO339_003253"
     CDS             3589..4098
                     /locus_tag="OO339_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5927772.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4134..5519)
                     /gene="cysS"
                     /locus_tag="OO339_003254"
     CDS             complement(4134..5519)
                     /gene="cysS"
                     /locus_tag="OO339_003254"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5927773.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5693..6187
                     /gene="ppiB"
                     /locus_tag="OO339_003255"
     CDS             5693..6187
                     /gene="ppiB"
                     /locus_tag="OO339_003255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5927774.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6190..6912
                     /gene="lpxH"
                     /locus_tag="OO339_003256"
     CDS             6190..6912
                     /gene="lpxH"
                     /locus_tag="OO339_003256"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5927775.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7030..7539
                     /gene="purE"
                     /locus_tag="OO339_003257"
     CDS             7030..7539
                     /gene="purE"
                     /locus_tag="OO339_003257"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5927776.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7536..8603
                     /gene="purK"
                     /locus_tag="OO339_003258"
     CDS             7536..8603
                     /gene="purK"
                     /locus_tag="OO339_003258"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5927777.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8740..9633)
                     /locus_tag="OO339_003259"
     CDS             complement(8740..9633)
                     /locus_tag="OO339_003259"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5927778.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9630..10277)
                     /locus_tag="OO339_003260"
                     /pseudo
     CDS             complement(9630..10277)
                     /locus_tag="OO339_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10280..11512
                     /gene="allC"
                     /locus_tag="OO339_003261"
     CDS             10280..11512
                     /gene="allC"
                     /locus_tag="OO339_003261"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5927779.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11523..>12162
                     /gene="allE"
                     /locus_tag="OO339_003262"
     CDS             11523..>12162
                     /gene="allE"
                     /locus_tag="OO339_003262"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5927780.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2949 a         3144 c         3103 g         2966 t
ORIGIN      
        1 atcaaagtta attgtggcat caggtacagc tttcagtaaa ttgatgcatt tattgttttg
       61 ctgctgggct aactcgggaa atgcactgct attaaggcca attcgttcaa ggagtacctg
      121 gttaaaacag ggcattaacc cacctgattt atcacctgta ttttctgttg tcgattcaaa
      181 tacgatatcc tgtgaaccaa tgaactcatc attacgccag agatccaccc gatacacgcc
      241 cgcaggttga tgattacctc tctcaaagcg cgataaatca gcaacggatg tgccattctc
      301 taataaaaat gccgggttga aataactttc cgcatagctt aaaaaggagg gtaaaatggc
      361 aagagatgaa taacaaattc cggccagaca ccaggtatac ctctgcggaa tatccgtagt
      421 agtgggtatt ttcataatta gctctgatat tgtcagatga ttcggttagt ttaagttcac
      481 ttctctgacc ggggttactg aaccataatc attaacggtc tgaaatttca gctttccctg
      541 cactcctgag ggtaagggaa tttgtgaact aactctgggt gccgccatag cattccccaa
      601 cttttggctg cccacttgca ggttaacgag ggtgacataa aaaggtgatg gattatgaac
      661 attgatatag ttaccggatc gcgaaaactt gagtgtgtcc ggcgcttctg cgggtaattc
      721 ttgtaattga attggacgga gaaataattt catgcgcgat aaaatcgcca gttgcaaaac
      781 attcctgccg ttcaatgcat ttttatctac cgaagggatc gtcttaacat tcatccagaa
      841 cagagactca cgatctgccg ccagcggtgg accggtgtaa ataatacgca aagtattttc
      901 gcttttgggt tcactaacaa atagtggcgg tgtaatgatg aatgactttt cttttacacc
      961 gctgctgttt tcaatccacg aattgaccag aaagagctca ttggtatggc tatttttaat
     1021 ccataccgca gtctgtttag catcagcggg ataaataata cgcgtggcac ctaatgcgat
     1081 ccctccggca gcatgggcgc tggccgaaat gattaaataa aatatcatga gcatcaataa
     1141 ctttatttta gtcatcatag ttaatacgcc ttgtgtattc ctgacggcaa aaataaggag
     1201 tataaagaaa tgattgcttt ggcttcttac aacacatcat tcccttttca tttaataaag
     1261 cggcatgata caaagtgaac gtggtttcga tttacattgc caccataccg cttcagacaa
     1321 ccggtacatt actttgttat aagacgtgat atataattca aaacaacgtg gttttgatta
     1381 ttcgtatctc ataataaaag tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc
     1441 ggtgccttta taacgtgcag aaaaatgaag aacgttggtc ccggggatca agttctggtt
     1501 ggttgagaag ctattcccat caggtttgag aattgcggat gtattatcaa gtatttcaat
     1561 accgacaccc gttgctgttg ttgtatttgt actggataca atggccaata aattatcatt
     1621 gattgcatca gcctgaccag aaaatgcaac ggcagcatta gcggcaacaa caggatcgca
     1681 gtcgttcaac tgaatggtga atggaattaa tgcagaggta ttaccaacag cattgaaaat
     1741 atcggtacga tattgaccga gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca
     1801 ggcagcattc accagttcgc cttcaaagtg aattgtaccg ccatctacaa cggcagcata
     1861 actacccgta gcggcgaata gtgcggcagc cagcgcagac gaaataaatc ttaatttcat
     1921 atatattcct tcaatctcat ttatcgactc cacatccgta tataaccgat tactttattt
     1981 aagacactga tagtagtaaa attccttttt atcctctaag aatgtcttaa ttgaaaatat
     2041 gcactctatt ctaaaaaata gagagccccg ttagatgaat acttccgcgc aaaatatatt
     2101 caccacaaat atagacctga agcggtaaat taccaggctg aaaattcttt ttatattgtc
     2161 aggtatttct taaattatct taatccttaa acaaggaaat aaatcagttc cagatttaca
     2221 acgccatcat gtacgaaaaa tgaagctttc aatctcagcg acctcagcga cggtgcgcct
     2281 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
     2341 gcttctgaca aaatagacgc atccccttcg acctacgtaa cagatggaat cctctctctg
     2401 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
     2461 caaaaagttc aggctcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
     2521 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
     2581 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgctg
     2641 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
     2701 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
     2761 gacggtttcc atccttacaa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
     2821 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctc
     2881 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgcc caatgagcat ggaactgttg
     2941 ctcgcaggtt gcaccaccac cgtgactcac cgcttcacta aaaatctgcg tcatcacgta
     3001 gaaaatgccg atctgttgat cgttgccgtt ggcaagccag gctttattcc cggtgactgg
     3061 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
     3121 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccc
     3181 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt
     3241 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     3301 cgttgagctg tgcgacttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     3361 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     3421 caaaatcgtc gccggtcaga cagtgagttt tgcaggacac agcgtacagg ttgttgcctg
     3481 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     3541 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     3601 attacgcacg ccgccgttcc cctttgcatt ggtttagggt tgggttcgaa aggtctgtta
     3661 tttgccggaa tcatcctggc gatgctgccc gatgccgacg tattgtcgtt taaatttggc
     3721 gttgcttacg gcaatgtttt tggtcatcgc ggttttaccc attcgctggt gtttgcgttt
     3781 gttgtcccgc tcttatgtgt gctgattgga cgtcgatggt tcagggctgg gctgattcgc
     3841 tgctggctgt ttttaaccgt ctcgttgcta tcgcacagct tactggattc ggtaaccact
     3901 ggcggtaagg gcgttggctg gctgtggccg tggtcagatg aacgcttttt cgctccctgg
     3961 caggtgatta aagtcgcgcc gtttgcattg tctcgttaca ccacagcgta cgggcatcag
     4021 gtgattattt ccgaattgat gtgggtgtgg ttgccgggga tgctactgat gggaatgttg
     4081 tggtggcgca gacgataacc ggatgcgaaa actcgcatcc ggcaattgcg caattactta
     4141 cgacgccagg tggtcccttg cgggccatct tccagcacga tccccatctc gttaagacgg
     4201 tcacgtgccg catctgccgc cgcccagtct ttcgctttac gggcatccag acgctgttga
     4261 attaacgctt caatctcagc cacttcgctg tcgtctgcct gcgcgccgct ttgcaggaac
     4321 gcttccggtt cttgctccag caggcccaat acagcggaaa gtttacgcag gtgagatgcc
     4381 attgcattcg ccgctgccat atcttctgct ttcagacggt tcacttcacg cgccatatca
     4441 aacagcacgg aataggcttc cggggtgttg aaatcgtcgt ccatcgcttc aataaagcgc
     4501 gcttcaaacg cttcgccacc ggcaggcgca acggttttat ctgtgccgcg cagcgcagtg
     4561 tagaggcgct ccagcgccgc acgcgcctgc ttcaggttct cttcgctgta gttcagctgg
     4621 ctgcgatagt ggcccgacat caggaagtaa cgcacggttt ccgcgtcgta gtatttcagc
     4681 acatcgcgca cggtaaagaa gttacccagc gatttggaca tcttctcgcg gtcaaccatc
     4741 accattccgg agtgcatcca gtagttcaca tactgaccat catgggcaca ggtggactgc
     4801 gcgatttcgt tctcgtggtg cgggaacatc aggtctgaac cgccgccgtg gatatcaaag
     4861 tggttaccca gctgcttgca gttcatggcc gaacattcaa tgtgccagcc aggacgaccc
     4921 gcgccccacg gagacggcca gctcggttcg ccctctttcg acatcttcca cagaacgaag
     4981 tccatccggt tgcgtttgtc atcaaccacg tcaacgcgcg cgccagcctg cagctggtcg
     5041 agatcctgac gcgacagcac gccataagtt ggatcggtcg ggacgtcgaa catcacgtcg
     5101 ctgttgtccg ccacataagc gtgacctttg gcgatcagtt gttcagtgag ttcaataatt
     5161 tctgcgatat ggtgcgtcgc gcgcggctcc atatccgggc gcagaatgtt caaagcatca
     5221 aaatctttgt gcatttcggc gatcatgcga tccaccagcg ccacaaagct ttcgccattt
     5281 tcattggcgc gtttgatgat tttgtcgtcg atatcggtaa tgttgcgcac atacttcagt
     5341 ttatagccga ggaaacgcag atagcgcgca accacgtcaa aagcaacaaa ggtacgcccg
     5401 tgaccgatat gacagagatc gtaaacggtg attccacaca cgtacatgcc gacttccccg
     5461 gcgtgaatag gcttaaattc ctctttttgg cgtgtcagag tattgaagat ttttagcatc
     5521 gaagattccg tttagacatg tgtgggtaat tgagttgcgt ataataccca tatttcccgc
     5581 gcgaatcagc atacattgcg tgatgatccg atcgtgcggt tatgctataa caccacccta
     5641 tatatgaccc gaactgggtt gaagcaccaa tcaaacggaa caggatgcaa aaatggttac
     5701 tttccacacc aatcacggcg atattgtcat caaaactttt gacgataaag cacctgaaac
     5761 agttaaaaac ttcctggact actgccgcga aggtttttac aacaacacca ttttccaccg
     5821 tgttatcaac ggctttatga ttcagggcgg cggttttgaa ccgggcatga aacaaaaagc
     5881 caccaaagaa ccgatcaaaa acgaagccaa caacggcctg aaaaataccc gtggtacgct
     5941 ggcaatggca cgtactcagg ctccgcactc tgcaactgca cagttcttca tcaacgtggt
     6001 tgataacgac ttcctgaact tctctggcga aagcctgcaa ggttggggct actgcgtgtt
     6061 tgctgaagtg gttgacggca tggacgtggt agacaaaatc aaaggtgttg caaccggtcg
     6121 taacggtatg caccaggacg tgccaaaaga agacgttatc attgaaagcg tgaccgttag
     6181 cgagtaatcg tggcgacact ctttattgca gatcttcatc tctgcgtgga agaaccggcg
     6241 atcaccgccg gttttctgcg ttttttagcg ggggaagccc gcaaggccga cgcgctgtat
     6301 attcttggcg atctgtttga agcatggatt ggcgacgacg atcccaaccc actccatcgc
     6361 cagatggcgg cagcgatcaa agcggtgtcc gattccggcg ttccctgtta tttcattcat
     6421 ggcaaccgtg attttctgct cggcaaacgc tttgcccgtg aaagcggcat gacattattg
     6481 ccggaagaaa aggtgctcga actttatggt cgccgggtgt tgattatgca tggcgacacg
     6541 ctgtgcaccg atgacgcggg ttatcaggct tttcgcgcca aagtccacaa accctggctg
     6601 caaatgctat tcctcgccct gccgttgttt gtgcgcaaac gcattgccgc gcgaatgcgc
     6661 gcgaacagca aagaagccaa cagcagtaaa tcgctggcga tcatggacgt taaccaaaac
     6721 gctgtggtca gtgcgatgga aaaacatcag gtgcaatggc taatccacgg gcatacccat
     6781 cgcccggcgg tgcatgaact tatcgccaac cagcaacctg cttttcgcgt ggtactgggt
     6841 gcctggcata cggaaggttc gatggtgaaa gtcacggctg atgacgttga gctgattcat
     6901 tttccgtttt aaaaaaccca caactttgct gatttcacag ccacgcaacc gttttccttg
     6961 ctctctttcc gtgctattct ctgtgccctc taaagccgag agttgtgcac cacaggagtt
     7021 ttaagacgca tgtcttcccg caataatccg gcgcgtgtcg ccatcgtgat ggggtccaaa
     7081 agcgactggg ctaccatgca gttcgccgtc gaaatcttcg aaatcctgaa tgtcccgcac
     7141 cacgttgaag tggtttctgc tcaccgcacc cccgataaac tgttcagctt cgccgaaagc
     7201 gccgaagaga acggttatca ggtgattatt gcgggcgcag gcggcgcagc gcacctgcca
     7261 ggcatgattg ccgccaaaac gctggtgccg gtgctgggcg tgccagtaca gagcgccgca
     7321 ctgagcggtg tcgatagcct ctactccatc gtacaaatgc cgcgcggcat tccggtgggt
     7381 acgctggcga ttggtaaagc tggcgcggca aacgcggcat tactggcagc acaaattctc
     7441 gcgactcacg ataaagagct acaccagcgt ctgaatggct ggcgcaaagc ccagaccgac
     7501 gaagtgctgg aaaacccgga cccgcgaggt gcggcatgaa acaggtttgc gtcctcggta
     7561 acgggcagtt aggccgtatg ctgcgtcagg caggcgaacc gttaggcatt gctgtctggc
     7621 cggtcgggct ggacgctgaa ccggcggcgg tgccttttca acaaagcgtg attaccgctg
     7681 agatcgaacg ctggccggaa accgcattaa cccgcgagct ggcgcgtcat ccggcctttg
     7741 tgaaccgcga tgtgttcccg attattgctg accgtctgac tcagaaacag cttttcgata
     7801 agctccacct gccgaccgcg ccgtggcagt tacttgccga tcgcagcgag tggcctgcgg
     7861 tgtttgatcg tttaggtgaa ctggcgattg ttaagcgtcg cactggtggc tatgacggtc
     7921 gcggtcaatg gagtttacgc gccaatgaaa ccgaacagtt accggcagag tgttacggtg
     7981 aatgtattgt cgagcagggc attaacttct ctggtgaagt atcgctggtt ggcgcgcgcg
     8041 gctttgatgg cagcaccgtg ttttatccgc tgacgcataa cctgcatcag gacggtattt
     8101 tgcgcaccag cgtcgctttt ccgcaggcca acgcacagca gcaggcgcaa gccgaagaga
     8161 tgctgtcggc gattatgcag gagctgggct atgtgggcgt gatggcgatg gagtgttttg
     8221 tcaccccgca aggtctgctg atcaacgaac tggctccgcg tgtgcataac agcggtcact
     8281 ggacacaaaa cggtgccagc atcagccagt ttgagctgca tctgcgggcg attaccgatc
     8341 tgccgttacc gcaaccagta gtgaataatc cgtcggtgat gatcaatctg attggtagcg
     8401 atgtgaatta tgactggctg aaattgccgc tggtgcatct gcactggtac gacaaagaag
     8461 tccgtccggg gcgtaaagtg gggcatctga atttgaccga cagcgacaca tcgcgtctga
     8521 ctgcgacgct ggaagcctta atcccgctgc tgccgccaga gtatgccagc ggcgtgattt
     8581 gggcgcagag taagttcagt taagcaggtg ctcatttgat tgccggatgc ggcgtaaacg
     8641 ccttatccgg cttaccgctc cggtacccat tgtaggcctg ataagacgcg tcaagcgtcg
     8701 catcaggcat ggtgcaccga ttgccggatg ctctcatcac tacagcgaaa tacaggtccc
     8761 cgcttcgccc gccagcgtct cttcaattcg cgataacgcc ccaatccacg cgggtttact
     8821 acggcttctg acataaccac tcaccgccgt taccttcggc cccatcgaac catcggcttt
     8881 ggcaaatggc gctaactcat ccggtgtggc atggcgaatg gcacgttgct gcggcgttcc
     8941 ccagttttca tataccgcat cagcatcggt gaggatcacc agtccatcgg cattaatctg
     9001 ctcggcgagc aacgcagcgg cgagatcttt atcaatcacc gcttcactcc ctgccccatc
     9061 ttccgtcaca ggcacaccgc cgccgccact gcaaatcacc acatgcccct ctttgagcaa
     9121 caactcgatg gcttcgctgt cgagaatttt acgcggttgc ggagacgcca ccacccggcg
     9181 caaatattta ccatcacgtt tcatctgcca gccgtaagcc gcttccagtg cctcttgttc
     9241 ttctggctga taaaccggac caataaattt ctctggctgc aaaaacgccg gatcatcagg
     9301 cgaaacctca atgcgcgtca gcaccgtcgt aacaggcggc atctgcggct gtgcgctcaa
     9361 gctctgcgcc agcatatagc caatcatccc ctggctttcc gccaccagta catccagcgg
     9421 atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca cctgcggccc
     9481 gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg cgggtacagc
     9541 actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct ggagtaaggc
     9601 gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa ttgttggcca
     9661 agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag cagcgaatca
     9721 atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag gagtggcgag
     9781 gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt ggtaacgagt
     9841 tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa agcgccataa
     9901 taccaggccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt taatccgggg
     9961 cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc ctgaaaacag
    10021 tgacgaaact ggcgtaattc agcgggcagc ggatcgcttg ccgccatcgc cagtggcccg
    10081 aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg aggatgcgaa
    10141 ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa acgcccgagg
    10201 cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc gcataacgca
    10261 tcgaattgcg tgcggcgaaa ttacacattc ccgtcaagct atagaagaaa cgctgccctg
    10321 gctttcctct tttggcgctg acccaacggg tgggatgacc cgtttacttt attcgccgga
    10381 atggctggaa acccagcagc aatttaaaaa aagaatggca gcaagtgggc tggaaacacg
    10441 tttcgatgaa gtggggaatt tatacggtcg cctgagtggc accgaatatc cacaggaagt
    10501 ggttctgagc ggttcgcata tcgataccgt ggttaacggc ggtaacctcg acgggcaatt
    10561 cggcgcgctg gcggcgtggc tggcaattga ctggttgaaa acgcaatacg gcgcaccgtt
    10621 acgtacggtc gaagtggtgg cgatggcaga agaagaaggc agccgcttcc cgtatgtctt
    10681 ctggggcagt aaaaatatct ttgggctggc gaatcctgac gacgtgcgga atatctgtga
    10741 tgccaaagga aatagttttg ccgatgcgat gaaggcttgc ggatttactc tgccgaacgc
    10801 cccactaact ccgcgtcagg ctataaaagc ctttgttgaa ctgcatattg aacagggctg
    10861 tgtgctggaa agtaatgggc aatcaattgg cgtggtgaat gcaattgtcg ggcagcgtcg
    10921 ctatacggtg acgctgaacg gcgaatcaaa ccatgcaggc accacgccga tgggttatcg
    10981 tcgtgatacg gtttacgctt tcagtcgcat ttgccatcag tcgatcgaaa aagcgaaaaa
    11041 gatgggcgat ccgctggttc tgacctttgg caaagtagag ccgcgcccga atacggtaaa
    11101 tgtggtgccg ggtaaaacca cgttcaccat tgattgtcgt cataccgacg ctgccgtgct
    11161 gcgcgatttc acccaacagt tagaaaacga catgcgggcg atttgcgatg aaatggacat
    11221 tggtattgat atcgatttat ggatggacga agaacccgtg ccgatgaata aggagctggt
    11281 cgccaccctg acagaattgt gtgaaagaga aaaactgaat taccgggtga tgcacagtgg
    11341 tgccgggcac gacgcgcaaa ttttcgcgcc tcgcgtacca acctgcatga tttttatccc
    11401 cagcatcaac gggatcagcc ataacccggc ggaacgcacc aatattactg accttgccga
    11461 aggggtcaaa acgttggcac tcatgcttta tcaacttgcc tggcaggaat aaggagtcat
    11521 aaatgggata tttaaataac gtcaccggtt accgcgaaga tttactggct aaccgtgcga
    11581 ttgttaaaca cggtaatttc gcactgttaa ccccggatgg tctgataaaa aatattattc
    11641 cggactttga aaattgtgac gcgacaatcc tctccacgcc aaagctgggt gcctcttttg
    11701 ttgattatct ggtcacactg catcaaaacg gtggcaacca acagggcttc ggtggcgaag
    11761 gcattgaaac gttcctgtat gtgatctctg gaaatatcac tgccaaagcc gaaggcaaaa
    11821 catttgcctt aagcgaaggt ggctatcttt attgcccacc aggctcctta atgacgtttg
    11881 ttaacgccca ggccgaagac agccaaatct ttttatataa gcgccgctat gttccggtag
    11941 aaggccatgc accgtggctg ttttctggca atgccagcga actggaacgc attcactatg
    12001 aaggtatgga tgatgtgatc ctgctggatt ttctgcccaa agagttaggt tttgatatga
    12061 acatgcatat cctctctttt gcaccaggtg ccagccacgg ttatatcgaa acacacgttc
    12121 aggaacatgg tgcctatatt ctttccggtc agggggttta ta
//
LOCUS       DAJTRH010000105        11959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTRH010000105 DAJTRH010000000
VERSION     DAJTRH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO339_003263"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO339_003263"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5927781.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO339_003264"
     CDS             complement(1806..3224)
                     /locus_tag="OO339_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5927782.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OO339_003265"
     CDS             complement(3378..3722)
                     /locus_tag="OO339_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5927783.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO339_003266"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO339_003266"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5927784.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO339_003267"
     CDS             complement(4712..5647)
                     /locus_tag="OO339_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5927785.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO339_003268"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO339_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5927786.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO339_003269"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO339_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5927787.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO339_003270"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO339_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5927788.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="OO339_003271"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="OO339_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5927789.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="OO339_003272"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="OO339_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5927790.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="OO339_003273"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="OO339_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5927791.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO339_003274"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO339_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5927792.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..>11959
                     /locus_tag="OO339_003275"
     CDS             11365..>11959
                     /locus_tag="OO339_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5927793.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFV"
BASE COUNT         3184 a         3096 c         2708 g         2971 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcacacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaa
//
LOCUS       DAJTRH010000106        11926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTRH010000106 DAJTRH010000000
VERSION     DAJTRH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..2342)
                     /locus_tag="OO339_003276"
     CDS             complement(<1..2342)
                     /locus_tag="OO339_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5927794.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OO339_003277"
     CDS             complement(2276..2899)
                     /locus_tag="OO339_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5927795.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OO339_003278"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OO339_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO339_003279"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO339_003279"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5927796.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OO339_003280"
     CDS             7507..8229
                     /locus_tag="OO339_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5927797.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO339_003281"
     CDS             complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO339_003281"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5927798.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10301..11896)
                     /locus_tag="OO339_003282"
     CDS             complement(10301..11896)
                     /locus_tag="OO339_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5927799.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3309 c         2945 g         2915 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta
    11641 aatcgacggc accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg
    11701 cgacgttacc ggaaaaaata acatcagtaa gattcagcgt tgagttttct ttagcaaata
    11761 tggccccgcc gttattatat tctcctgtaa cggtgttatt ggcaaacaga gtcattccag
    11821 tttcattttc tggtaatagt gaaaattccg ctccttgttg caaaaatacg gccccaccgc
    11881 tggcattatt ggtcatatcc gaaaaaacca gccattgccc atcggc
//
LOCUS       DAJTRH010000107        11822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTRH010000107 DAJTRH010000000
VERSION     DAJTRH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO339_003283"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO339_003283"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO339_003284"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO339_003284"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5927800.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO339_003285"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO339_003285"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5927801.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO339_003286"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO339_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO339_003287"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO339_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5927802.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO339_003288"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO339_003288"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5927803.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO339_003289"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO339_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5927804.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO339_003290"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO339_003290"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5927805.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO339_003291"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO339_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5927806.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO339_003292"
     CDS             9553..10332
                     /locus_tag="OO339_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5927807.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO339_003293"
     CDS             10322..11131
                     /locus_tag="OO339_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5927808.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO339_003294"
     CDS             11218..11652
                     /locus_tag="OO339_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5927809.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>11822
                     /locus_tag="OO339_003295"
     CDS             11649..>11822
                     /locus_tag="OO339_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5927810.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHG"
BASE COUNT         2825 a         3187 c         3001 g         2809 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gt
//
LOCUS       DAJTRH010000108        11713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTRH010000108 DAJTRH010000000
VERSION     DAJTRH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            125..994
                     /locus_tag="OO339_003296"
     CDS             125..994
                     /locus_tag="OO339_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5927811.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1197..1550)
                     /locus_tag="OO339_003297"
     CDS             complement(1197..1550)
                     /locus_tag="OO339_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5927812.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1688..3334)
                     /gene="groL"
                     /locus_tag="OO339_003298"
     CDS             complement(1688..3334)
                     /gene="groL"
                     /locus_tag="OO339_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5927813.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3378..3671)
                     /locus_tag="OO339_003299"
     CDS             complement(3378..3671)
                     /locus_tag="OO339_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5927814.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3947..5203
                     /gene="yjeH"
                     /locus_tag="OO339_003300"
     CDS             3947..5203
                     /gene="yjeH"
                     /locus_tag="OO339_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5927815.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5219..5695)
                     /gene="fxsA"
                     /locus_tag="OO339_003301"
     CDS             complement(5219..5695)
                     /gene="fxsA"
                     /locus_tag="OO339_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5927816.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6032..7468
                     /gene="aspA"
                     /locus_tag="OO339_003302"
     CDS             6032..7468
                     /gene="aspA"
                     /locus_tag="OO339_003302"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5927817.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7586..8887
                     /gene="dcuA"
                     /locus_tag="OO339_003303"
     CDS             7586..8887
                     /gene="dcuA"
                     /locus_tag="OO339_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5927818.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9003..9341
                     /gene="cutA"
                     /locus_tag="OO339_003304"
     CDS             9003..9341
                     /gene="cutA"
                     /locus_tag="OO339_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5927819.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9317..11014
                     /gene="dsbD"
                     /locus_tag="OO339_003305"
     CDS             9317..11014
                     /gene="dsbD"
                     /locus_tag="OO339_003305"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5927820.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11051..11626
                     /locus_tag="OO339_003306"
     CDS             11051..11626
                     /locus_tag="OO339_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5927821.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2814 a         3024 c         2881 g         2994 t
ORIGIN      
        1 tctgtttata tagagtgagc acggctattt atgttatttt aacaactatt actaccaagc
       61 ccatttatct ggcttataaa ttaaaattaa ttatttaatt gagaaatggt tagggagaac
      121 ctacatggcc ataagcatca agggtgttaa taccggtgtt attcgcaaga gtaataattt
      181 cattgcactg gcgctgaaaa ttaaagaacc tcgcaataaa gaatcgttgt tctttatgtc
      241 tgtcatggaa ctgcgcgacc tgctgattgc gctggaaagt cgtctgcatc aaaagcataa
      301 actggatgct gctgcacgcc ttcagtatga gcaagctcgc gataaagtca ttaagaaaat
      361 ggcagaaaac atcccagaaa ttctggttga tgagcttaaa aatgccgata ttaatcgtcg
      421 ggttaacacg ctggaactta ccgataatca aggcgaaaat cttaccttcg tattaacgct
      481 acatgacggt agcaaatgtg agttagtcgt caatgagttg cagattgaaa tgctggcgcg
      541 agcaatcatt catgcaatta ataatgcgga aatgcgtgaa ctggcattac gtattacctc
      601 actgttagat ttcctgccgt tatacgacgt tgactgccag gacaatggca atctggaata
      661 tgacacctat tcccaaccgg aatggaaaca taacttgttc gaccattatc tggcggtgct
      721 ttaccgcttt aaagatgaaa gtggcaaaga acaatttagc ggcgcagtgg tgaaaacgcg
      781 tgaagcaacg ccgggtaaag aaatagaggc gatcactcgc cgaatgcttg atttcagccc
      841 acgactgaaa aaactcgcgg gtgtgccatg tcaggtctat gttcgcaccg tagcggcgaa
      901 taacgcccaa ccgctaaccc aggatcaatg cttacgcgcg ctgcatcact tacgtgtaca
      961 atccaccagc aaaactgcac cacaggcgaa ataatctcat ctttgctaat gcgaggctgg
     1021 agccatccag cctgaaatga ctgataacgt gatcgttggt catgccggat gcggcgtaaa
     1081 cgccttatcc ggcctacaaa ttctcaagca ttcaatcagt tgcaatgcct gctgtaggcc
     1141 tgataagcga agcgcatcag gcaataatac gtttgtcacc cagcctctgg atcacatcag
     1201 ttcgggcact tataaacctg accgataatc tggctatccg tcgggacaaa actggacaac
     1261 attccctgcg acgggctgct gatgccataa atcacgttac cgcccattgc agccgcctgg
     1321 ttgcgcagat cgtttgctgc gccgcgcata gaaccgccct cttctccgtg ttgcccggaa
     1381 agccagttgc tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc
     1441 tcgtccacaa tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc
     1501 gccatcagta gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc
     1561 ataatcaatg agctggacgc cactcgcgtc atccgtgtct gaatcttata ctagaaagat
     1621 gaccaaaaga aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaagtgc
     1681 agggcaatta catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta
     1741 agtcagctgc atcgtttttc ggcaggtcgg taaccatgca ttcggtggtg atcatcaggc
     1801 cagccacaga agctgcgtac tgcagagcag aacgggttac tttggttggg tccaggatac
     1861 ccatgtcgat catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc
     1921 cgcctttaac ggtgttagca acaacagacg gttcttcgcc gcagttcagg acgatctgac
     1981 gcagcggagc ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct
     2041 gaccacgcag gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa
     2101 ccacgccttc ttctaccgca gcacgggtcg cgtgcagggc atcttcaacg cgtgcttttt
     2161 tctctttcat ttcaacttcg gtagcagcac ctactttgat aactgcaacg ccgcctgcca
     2221 gtttcgctac gcgctcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct
     2281 gctgacggat ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcgatga
     2341 tagtggtggt gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg
     2401 ctttttccag ctccataccg atctcttcag agattacggt accgccagtc agggttgcga
     2461 tatcctgcag catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt
     2521 tcacgatgcc acgcatggtg ttaacaacca gagttgctag cgcttcgcct tcaacatctt
     2581 cagcgatgat cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt
     2641 cgcggatgtt ggagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg
     2701 cgccagtttc cggcttgttg atgaagtaag gagacaggta gccacggtcg aactgcatac
     2761 cttcaaccac gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt
     2821 ctttaccgac tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag
     2881 cggagatggt accaacctga gcaatcgctt tagaatcaga gcacggtacg gacagcgctt
     2941 tcagttcttc aactgcagcg gtaaccgctt tgtcgatacc acgtttcagg tccatcgggt
     3001 tcatgcccgc agcaacagct ttcagacctt cagtgatgat agcctgagcc agtacggttg
     3061 cagtggtggt accgtcgcct gcagcgtcgt tcgctttgga ggcaacttct ttcaccatct
     3121 gcgcacccat attttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat
     3181 ctttggtgat ggtcggtgca ccgaaagatt tatccagaac tacgttacgg ccttttggac
     3241 cgagggtaac tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag
     3301 cgtcgttacc gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt
     3361 tcagtgtcgt gcgcggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc
     3421 aacacttctt cattgtcgat cttctcggat ttcacaccgt agccatcgtt gaaaataacg
     3481 atgtcgccaa ctttcacgtc cagcggcttc acttcgccat tttcaaggat acggccattg
     3541 ccgacagcca gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg
     3601 ccgccagcag atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga
     3661 cgaatattca ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga
     3721 gcttacgtgg tttcccggct ggtgaccaga gaaatgggga tgaggcttcg ccccttcaag
     3781 ggggaaaaag aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct gattcacctc
     3841 acatttcatc gcaatttctt catcgccgtc ataagcgaat ctgattgtgc taccatcgaa
     3901 aatctacgca gttgcccaaa atttgggcgc aatcggatat caacccatga gtggactcaa
     3961 acaagaactg gggctggccc agggcattgg cctgctatcg acgtcattat taggcactgg
     4021 cgtgtttgcc gttcctgcgt tagctgcact ggtagcaggc aataacagcc tgtgggcgtg
     4081 gcccgttttg attatcttag tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca
     4141 ctatcccagc gcaggcggcg tcgcgcactt cgtcggtatg gcgtttggtt cgcggcttga
     4201 gcgagtcacc ggctggctgt ttttatcggt cattcccgtg ggtttgcctg ccgcactaca
     4261 aattgccgcc ggattcggcc aagcaatgtt tggctggcat agctggcaac tgttgttggc
     4321 agaactcggt acgctggcgt tggtgtggta tatcggtact ctcggtgcca gttccagtgc
     4381 taatctacaa accgttattg ccggacttat cgtcgcactg attgtcgcta tctggtgggc
     4441 gggcgatatc aaacctgcga gtatcccctt ccccgcgcca ggaaatatcg aacttaccgg
     4501 gttatttgct gcgttatcag tgatgttctg gtgttttgtc ggtctggaag catttgccca
     4561 tcttgcctcg gaatttaaaa atccagagcg tgattttcct cgtgctttga tgattggtct
     4621 gctgctggca ggattagtct actggggctg tacggtagtc gtcttacact tcgacgccta
     4681 tggtgaacaa atggcggcgg cagcatcgct tccaaaaatt gtagtacagt tgttcggtgt
     4741 aggagcgtta tggattgcct gcgtaattgg ctatctggcc tgctttgcca gtcttaacat
     4801 ttatatacag agcttcgccc gcctggtctg gtcgcaggcg caacataatc ctgaccacta
     4861 cctggcacgc ctctcttctc gccatatccc gaataatgcc ctcaatgcgg tgcttggctg
     4921 ctgcgtggtg agcacgttgg taattcatgc cttagagatc aatctggacg ctcttattat
     4981 ttatgccaat ggcatcttta ttatgattta tctgttatgc atgctggcag gctgtaaatt
     5041 attgcaaggt cgttatcgac tactggcggt ggttggcggg ctgttatgcg ttctgttact
     5101 ggcaatggtc ggctggaaaa gtctctatgc gctgatcatg ctggtggggt tatggctgtt
     5161 tctgccaaaa cgaaaaacgc cggaaaatgg cataaccaca taatccggcg tttcgacatt
     5221 aatcctggcg atcgtcttta tgatcaaggc ggtcgcggtc atcatccttt cgctgatact
     5281 caccatcaaa agtattaccg ccaccggtcc cggcgctaaa accgccgcct ggcatgcgag
     5341 aaaagcgcaa atgtggcatc aacttcactg tcagatgctt ttgcaccggc ggcaataaaa
     5401 gcagaagacc gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa ccggcaataa
     5461 tcagcgaaac acttttaatc atctccgccg cagggttttc acccgccgcc attttttgct
     5521 gcatcagcac gaaattctta aagccctggt tacgtaccag tgacataccg ataactgacg
     5581 tgaatataac cagcacgagg gtcagcaata cccccaatac atgggcaacc tgaataaaga
     5641 ttgaaatctc aatatagaca taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt
     5701 tctcctgtaa tagcagccgg ttaaacccgg ctacctgaat gggttgcgaa tcgccttcag
     5761 cttatattgt ggtcattagc aaaatttcaa gatgtttgcg caactatttt tggtagtaat
     5821 cccaaagcgg tgatctattt cacaaattaa taattaaggg gtaaaaaccg acacttaaag
     5881 tgatccagat tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc
     5941 aggggataat cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca
     6001 aatcattggc agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga
     6061 tctgttgggt accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc
     6121 gattgaaaac ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg
     6181 tatggtaatg gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa
     6241 aagtgtagcg aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat
     6301 ggatcagttc ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac
     6361 caacgaagtg ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca
     6421 gtacctgaac ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac
     6481 cggtttccgt atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact
     6541 gcgtgaaggc tttgaacgta aagctgtcga attccaggac atcctgaaaa tgggtcgtac
     6601 ccagctgcag gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct
     6661 gctgaaagaa gaagtgaaaa acatccaacg taccgctgaa ctgctgctgg aagttaacct
     6721 tggcgcaaca gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt
     6781 gaaaaaactg gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc
     6841 gacctctgac tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa
     6901 gatgtccaaa atctgtaacg acctgcgctt gctctcttct ggtccacgtg ccggcctgaa
     6961 cgagatcaac ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc
     7021 ggttgttccg gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt
     7081 taccatggca gcagaagcag gtcagctgca gttgaacgtt atggaaccgg tcattggcca
     7141 ggccatgttc gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg
     7201 cattaacggc atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg
     7261 tatcgttact tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat
     7321 ctgtgccgaa accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga
     7381 agcggaactt gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa
     7441 acgctatact gatgaaagcg aacagtaatc gtacagggta gtacaaataa aaaaggcacg
     7501 tcagatgacg tgcctttttt cttgtgagca gtaacttaaa aataacaatc taatatcaac
     7561 ttgttaaaaa acaaggaagg ctaatatgct agttgtagag ctcatcatag ttttgctggc
     7621 gatcttcttg ggcgccagat tggggggaat aggtattggt tttgcaggcg gattgggggt
     7681 gctggttctt gccgctattg gcgttaaacc cggtaacatc ccgttcgatg tcatttccat
     7741 tatcatggcg gttatcgccg ctatttctgc catgcaggtt gctggcggtc tggactatct
     7801 ggttcatcag acagaaaagc tgctgcgccg taacccgaaa tacatcacga tcctcgcacc
     7861 gatcgtgacc tatttcctga ctatctttgc tggtactggc aacatctctc tggcgacact
     7921 gccagttatc gctgaagttg cgaaggaaca aggcgttaaa ccttgccgtc cgctctctac
     7981 tgcagtggta tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg cagtggttta
     8041 catgtcttcc gtgatggaag gtcatggcat cagctacctc catctgctct ccgtggtcat
     8101 cccgtccacc ctgctggcgg ttctggtgat gtccttcctg gtcactatgc tgttcaactc
     8161 caaactctct gacgatccga tttatcgcaa gcgtctggaa gagggcttgg ttgaactgcg
     8221 cggtgaaaag cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct
     8281 gggcgtagtt ggcgtggtta tctatgcaat catcaacagc ccaagcatgg gtctggttga
     8341 aaaaccgctg atgaacacca ccaacgcaat cctgatcatc atgctcagcg ttgcaactct
     8401 gaccaccgtt atctgtaaag tcgataccga caacatcctc aactccagca ccttcaaagc
     8461 aggtatgagc gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc
     8521 caacaacatc gactggatca aagataccgc tggtgaagtg attcagggtc atccgtggct
     8581 gctggccgtc atcttcttct ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa
     8641 agcactgatg ccgatggctc tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt
     8701 cgctgcggtg tctggtctgt tcattctgcc gacctacccg acgctggttg ctgcggtaca
     8761 gatggatgac acgggtacta cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat
     8821 cccgggtact ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat
     8881 gctgtaatga tcaatcgcgg ggcgttcacg ccccgctttc tttcccgccg actaacatcc
     8941 ttcccccgtc cgttgtatag tgacctctct cttgcggttc catctgttct tgcgaggtgt
     9001 ttatgcttga tgaaaaaagt tcgaatacca cgtctgtcgt ggtgctatgt accgcaccgg
     9061 atgaagcgac agcccaggat ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg
     9121 cgaccttgat ccccggcgct acctctctct attactggga aggtaagctg gagcaagaat
     9181 acgaagtgca gatgatttta aaaactaccg tatctcacca gcaggcactg ctggaatgcc
     9241 tgaagtctca tcatccatat caaaccccgg aacttctggt tttacctgtt acacacggag
     9301 acacagatta cctctcatgg ctcaacgcat ctttacgctg attctgctac tttgcagcac
     9361 ttccgttttt gccggattat tcgacgcgcc gggacgttca caatttgtcc ccgcggatca
     9421 agcctttgct tttgattttc agcaaaacca acatgacctt aatctgacct ggcagatcaa
     9481 agacggttac tacctctacc gtaaacagat ccgcattacg ccggaacacg cgaaaattgc
     9541 cgacgagcag ctgccgcaag gcgtctggca tgaagatgag ttttacggca aaagcgagat
     9601 ttaccgcgat cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt
     9661 aactgtcacc taccagggct gtgctgatgc cggtttctgt tatccgccag aaaccaaaac
     9721 cgttccgtta agcgaagtgg tcgccaacaa cgcagcgtca cagcctgtgt ctgtttcgca
     9781 gcaagagcag cacaccgcgc aattgccctt ttccgcgctc tgggcgttgt tgatcggtat
     9841 tggtatcgcc tttacgccat gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct
     9901 gggtggtaaa cagcggctct ccactgccag agcattgttg ctgaccttta tttatgtgca
     9961 ggggatggca ctgacttaca cggcgctggg tctggtagtt gccgccgcag ggttacagtt
    10021 ccaggcggcg ctacagcacc catacgtgct cattggcctc gccatcgtct ttaccttgct
    10081 ggcgatgtca atgtttggct tgtttactct acaactcccc tcttcgctgc aaacacgtct
    10141 cacgttgatg agcaatcgcc aacagggcgg ctcacctagc ggtgtgtttg ttatgggggc
    10201 gattgccgga ctgatctgtt caccatgcac caccgcaccg cttagcgcga ttctgctgta
    10261 tatcgcccaa agcgggaaca tgtggccggg cggcggcacg ctttatctct atgcgttggg
    10321 catgggcctg ccgctgatgc taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg
    10381 cccgtggatg gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt
    10441 cctgctggag cgagtgattg gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt
    10501 cgcattcttt ggctgggcct ttatcaccag cctacaggct aaacgcggct ggatgcgcgt
    10561 ggtgcaaata atcctgctgg cagcggcatt ggttagcgtg cgcccacttc aggattgggc
    10621 atttggtgcg acgcataccg cgcaaactca aacgcatctc aactttacac aaatcaaaac
    10681 ggtagatgag ttaaatcagg cgctcgttga agccaaaggc aaaccggtga tgttagatct
    10741 ttatgccgac tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca gcgacccgca
    10801 ggtgcaaaaa gcgttagcag acacggtctt acttcaggcc aacgtcacgg ccaacgacgc
    10861 acaagatgtg gcgctgttaa agcatcttaa tgtccttggc ctaccgacaa ttctcttttt
    10921 tgacggacaa ggccaggagc atccacaagc acgcgtcacg ggctttatgg atgctgaaac
    10981 cttcagcgca catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga
    11041 ggaaaatagc gtgcaacgtg aagatgtact gggagaagcc ctgaaattat tagaattaca
    11101 agggattgcc aacaccacgc tggagatggt tgctgaacgt gtggattatc cactggacga
    11161 gctacgccgc ttctggccag acaaagaggc aatcctctac gatgcgctgc gttatcttag
    11221 ccaacagata gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca
    11281 aaagctgctg gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg
    11341 ctgtctattt atcgctgcct gtacgtttta tcccgatcct ggccacccta ttcatcaact
    11401 ggccgatcag caaaaaagcg ctgcctacga tttcacccac gaactgttaa ccacgctgga
    11461 agttgacgat ccggcgatgg tagcaaagca gatggaactg gtgctggaag gctgtttaag
    11521 ccgaatgctg gtgaatcgta gccaggcgga tgtcgacact gcacatcggc tggcggaaga
    11581 tatcctgcgc ttcgcccgct gccgccaggg tggtgcattg acctgacaga aacacagaaa
    11641 agaagcgatt tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt aggttgacga
    11701 gatgtgcaga tta
//
LOCUS       DAJTRH010000109        11699 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTRH010000109 DAJTRH010000000
VERSION     DAJTRH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..363
                     /locus_tag="OO339_003307"
     CDS             <1..363
                     /locus_tag="OO339_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5927822.1"
                     /translation="GSITGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDR
                     AVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRL
                     SEQIQNDLLLRATGGVCV"
     gene            complement(480..755)
                     /gene="yjfN"
                     /locus_tag="OO339_003308"
     CDS             complement(480..755)
                     /gene="yjfN"
                     /locus_tag="OO339_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5927823.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(904..1233)
                     /gene="bsmA"
                     /locus_tag="OO339_003309"
     CDS             complement(904..1233)
                     /gene="bsmA"
                     /locus_tag="OO339_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5927824.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1415..2164
                     /gene="yjfP"
                     /locus_tag="OO339_003310"
     CDS             1415..2164
                     /gene="yjfP"
                     /locus_tag="OO339_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5927825.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2161..2916)
                     /gene="ulaR"
                     /locus_tag="OO339_003311"
     CDS             complement(2161..2916)
                     /gene="ulaR"
                     /locus_tag="OO339_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5927826.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3024..4088)
                     /gene="ulaG"
                     /locus_tag="OO339_003312"
     CDS             complement(3024..4088)
                     /gene="ulaG"
                     /locus_tag="OO339_003312"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5927827.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4443..5840
                     /gene="ulaA"
                     /locus_tag="OO339_003313"
     CDS             4443..5840
                     /gene="ulaA"
                     /locus_tag="OO339_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5927828.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5856..6161
                     /gene="ulaB"
                     /locus_tag="OO339_003314"
     CDS             5856..6161
                     /gene="ulaB"
                     /locus_tag="OO339_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5927829.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6171..6635
                     /gene="ulaC"
                     /locus_tag="OO339_003315"
     CDS             6171..6635
                     /gene="ulaC"
                     /locus_tag="OO339_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5927830.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6649..7299
                     /gene="ulaD"
                     /locus_tag="OO339_003316"
     CDS             6649..7299
                     /gene="ulaD"
                     /locus_tag="OO339_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5927831.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7309..8163
                     /gene="ulaE"
                     /locus_tag="OO339_003317"
     CDS             7309..8163
                     /gene="ulaE"
                     /locus_tag="OO339_003317"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5927832.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8163..8849
                     /gene="ulaF"
                     /locus_tag="OO339_003318"
     CDS             8163..8849
                     /gene="ulaF"
                     /locus_tag="OO339_003318"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5927833.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8978..9253)
                     /locus_tag="OO339_003319"
     CDS             complement(8978..9253)
                     /locus_tag="OO339_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5927834.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9580..9975
                     /gene="rpsF"
                     /locus_tag="OO339_003320"
     CDS             9580..9975
                     /gene="rpsF"
                     /locus_tag="OO339_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5927835.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9982..10296
                     /gene="priB"
                     /locus_tag="OO339_003321"
     CDS             9982..10296
                     /gene="priB"
                     /locus_tag="OO339_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5927836.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10301..10528
                     /gene="rpsR"
                     /locus_tag="OO339_003322"
     CDS             10301..10528
                     /gene="rpsR"
                     /locus_tag="OO339_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5927837.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10570..11019
                     /gene="rplI"
                     /locus_tag="OO339_003323"
     CDS             10570..11019
                     /gene="rplI"
                     /locus_tag="OO339_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5927838.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11090..11653)
                     /locus_tag="OO339_003324"
                     /pseudo
     CDS             complement(11090..11653)
                     /locus_tag="OO339_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2770 a         2876 c         3092 g         2961 t
ORIGIN      
        1 ggcagcatta ccggttccgg caatattatg tgcctggatg tgttgcgcgt tctcaataag
       61 caagcgggcg tatacgactt attgtcggaa gcatttgtgg aagtgaaagg gcaggatcgc
      121 tattttgatc gcgcggttcg tcgtttacag cagcagctgc gtaagccagc tgaagaactg
      181 gggcgagaga ttactcatca gctattcctg ctgggctgcg gtgcgcaaat gttgaaatat
      241 gcttctccgc caatggcgca ggcgtggtgt caggtgatgt tagatacgcg cggcggcgta
      301 cggttgtcag agcagatcca gaatgattta ttgctgcggg cgacgggggg agtgtgtgtg
      361 taagcgtata cgactgatgc gacgctggtt tcgattaact aaatgaaata tgtgaaaatt
      421 gtaggccgga caaggcgctc gcgccgcatc cggcattgtt catttgctgt agatgacact
      481 catgcataca gtatcgcctg tacgcgccag ttatcaggct gctggtcttc gtacatggta
      541 ataatgcggt accatgacgc accttgttca tcggccttta acgccacgat acgttccacg
      601 tcctgcggat cccctgaaat attgctaact gagatctgac ctatttcatt caggcccgtc
      661 acgcagtcag cactggcgaa ttctgcagac tgtgccgtgg cgctcgcagg ataagtcact
      721 aattgcagcg agggtgaggc aagtaattgt ttcatcgtca gctccatttt tcgtttcccc
      781 acatgttgca gggcattcca tcgcggtaat aaacgcccaa gcttccagcc ggtaatttat
      841 tgtgcacagg taaacgcata gggacgcaaa gcaatgtaaa gctgactatg taagttgtct
      901 gatttattta cggtacaaaa tggcttgtga ataccactgt cctgtcacga tggtttcgtc
      961 caccatgacg acaacgtaat aatcagcttt tgcagcgaca gcttttgctc tgatttctgc
     1021 taatgcgtca tccggagaac cccgaaccat cgtgcttacg ctacctattc gctgtaaccc
     1081 ttgcgtctgg tcgcggcgaa tctcttgcgg atggtccgtt actggcggtg ctggctgtgg
     1141 cgtaccttgc agtgcgctac aggcacttaa catcaacacc aataataaac tggcaaaccg
     1201 gtaaataacg ctattacgtt tcctgctaac catagtgtag tgccttatta atttaacttt
     1261 gggggaatgt tcccgaattg ttacctggta cgcgattttc gaatgaaaac gtgacgaaag
     1321 cgtaacccac atctcaacat tatgaactaa taccgccagc agaaacttgt gctaaggatt
     1381 tcttcatgtc ataccagaca aaaaggagag acagatgatt gaaatagaat cacgcgagct
     1441 ggcagatatt cccgttcttc atgcttatcc tgtcgggcaa aaagataccc cgttaccgtg
     1501 cgtaattttt tatcacggct ttacttcatc cagcctggtg tatagctatt ttgccgttgc
     1561 gctggcgcag gctggtttgc gggtgatcat gccggatgcg cccgatcacg gtagccgttt
     1621 tagtggcgac gaagcgcggc ggttaaatca attctggcaa atcttgctac aaagtatgca
     1681 ggaattcact actttacgtg cggcaatagc cgaagaaaac tggctgcttg atgaccgtct
     1741 ggcagtcggt ggcgcgtcga tgggcgcgat gacggcactg ggaattaccg ctcgccaccc
     1801 cacggtgaaa tgtaccgcca gcatgatggg atcgggctat tttacatcgc tcgcccgttc
     1861 actgtttcca ccgctgatac ctgaaacggc agcacagcag aatgaattca ataaaattgt
     1921 cgcgccgctg gcagagtggg aagcgacaaa ccacctggaa caacttgggg acagacctct
     1981 actgctgtgg catggcctcg acgatgacgt tgtgcctgcc gacgaatcac tacgtttgca
     2041 gcaggcctta agcgagacgg gacgggataa actgctaacc tgttcatggc agccaggcgt
     2101 gcgtcaccgc attacgcctg aggcgttaga tgctgccgtg acatttttcc gccagcatct
     2161 ttaaacacgc agaatgctga ccccttgcgc ttccagttgt tgcaggattt ccgggttagc
     2221 atttttgccg gtgatcagca tatcgatttg atcggcacgg ctaaaaagca ttcccgcgcg
     2281 ttcgccaatc ttactgctat caaccagtac caccagtttc cctaccacgc tcagcatctt
     2341 ctgctctgcc attgctgtca gcatatcggt tttatacaac ccttctgcgg tcagcccttt
     2401 tccgctggta aacatccagt gcccggcata gagactgttt tcgctgccct gcgggcttaa
     2461 agtgatggac tgacttttgt tgtactgtcc acccataatg atcacgctgt catgttcttg
     2521 atcgatcagg taatttgcca gcggtagata attagtgatg atttgcactg gcttgccaca
     2581 catttcccgc ccaagcagaa acgcggtgga gccgcagttg atgactacgc tttcgcccgg
     2641 attaaccagc tgcgaggccg ctttagcaat acgtactttt tcatcgtgat tctgcgcctg
     2701 atgcagattc atcggcgtcc agcgcgggcg ctgttgggta atagcttctg cgccattgcg
     2761 cacctttttc agtttgccgc tttcgccaag tttattgata tcgcgtcgcg cagtggcagg
     2821 cgaaattccc agacgctcaa cgactttctc aacggtcaca aagcccaatt gtgcgagcat
     2881 ttccaggagg atttgatgtc tttgtgcttc agtcatgagc tattccgata aaatttgatt
     2941 tttttagatg atatttgaaa tagccaggaa atactacgcc ggatagcgcg aacttctcca
     3001 cgctatccgg tataaacgac agattacagg aatgacttga acggcagatc cggttcaata
     3061 gtgaagcaat catcgaaacc gcgcggatag tggtactcga agttgtcttt atccagcggc
     3121 caggtaaatt tgccgccaac ctgccagata aacggcttga agccatactt cagacgatct
     3181 tttttgatct cccacagcac gcggatctct tgcggatcgg cctggaagtt tgaccagatg
     3241 tcgtggtgga acgggataac cactttcgca ttcagggctt cacccatacg cagcatatcg
     3301 gcgctggtca ttttgtcggt aataccgcgc gggttctcac catacgatcc taacgccaca
     3361 tcgatctgat gttcgttacc gtgcttcgca taatagttag agtagtggga atcgccactg
     3421 tgatataggg aaccgccagg ggttttgaac aggtagttta ctgcgcgatc gtccatgcca
     3481 tctggcagta cgccagccgc tttttgatcg gcaggcaggg tgatcagtgc agtacggtcg
     3541 aaagcgtcaa gcgcatgaat ttcaatgtct ttcactttta caacatcgcc cggtttgacc
     3601 acgatgcaac gctctttcgg tacgccccag ccaatccaca aatcaacgca ggttttcggt
     3661 ccaatgaatg gtacgtcatc agcacagttc tgcatcacgg cagcagcgac gttaacgtcg
     3721 atatgatcgt tgtgatcgtg agtcgccagt accgcgtcga tctggcgaat cgcaaacgga
     3781 tcaagaacaa acggggtggt gcgtaggttt ggctgcagtt ttttcacgcc agccatgcgc
     3841 tgcatctggt gaccctgttt cattaacggg ttaccgtgac tttgtttgcc agtgccgcac
     3901 cagaaatcaa cgcaaacgtt ggtgccacct tctgatttca accagatccc ggtgcagcct
     3961 agccaccaca tcgcaaatgt gccaggggcg acctgctctt gttcaatttc ttcattcagc
     4021 cagctacccc actccgggaa agtgctcagg atccaggatt ctcgggtgat acttttcact
     4081 ttactcatcg ccatttacct tcatgatagt tcaattcgaa tcaacatgtg attggttttg
     4141 attaatcctg acactattcc ttcaggaagg taatgaccat tttttgactt tttgccaggg
     4201 aagttgttgt tgattttgag tggggaaaga tttaatggaa tgtgtagtta aattaactaa
     4261 atgaatttaa ataaataatt gtttcgttgt gtgaatccca ctctatccat gtggaattat
     4321 ttgcgggtcg cgtcacattt aatcataaat aatcttgttg tgattacttt tgaaaattag
     4381 agtgagtgca caacattccg ggtgtgcgga atacccggtt acctcttctt caggagatcg
     4441 ttatggagat cctctacaac atctttaccg tgttttttaa ccaggtcatg accaatgccc
     4501 cgttgttgct gggtattgtg acctgtctgg gctacatcct actgcgcaaa agtgtcagcg
     4561 ttattattaa aggcacgatt aaaaccataa ttggtttcat gttgttgcag gcagggtccg
     4621 gcatcctcac cagcaccttc aaaccggtgg tggcgaaaat gtccgaagtc tacggcatta
     4681 acggcgcaat ttccgatact tacgcgtcga tgatggcaac catcgaccgc atgggcgatg
     4741 cctatagctg ggtaggttac gcggtgttgt tagcgctggc gctgaacatc tgttacgtgc
     4801 tgctgcgtcg cattaccggt attcgtacca tcatgctgac tggtcatatc atgttccagc
     4861 aggcggggct gattgccgtt acgttgttta ttttcggcta ctccatgtgg acaaccatta
     4921 tctgcacggc gattctggtt tcgctctact ggggcattac ctccaacatg atgtacaagc
     4981 cgactcagga agtgacggac ggctgcggtt tctccatcgg tcaccagcaa cagtttgcat
     5041 cattaattgc ctataaagtt gcgccgttcc tcggcaaaaa agaggaaagc gttgaagacc
     5101 tcaaactgcc aggctggctg aatattttcc acgacaacat cgtctccacg gcgattgtca
     5161 tgaccatctt ctttggtgcc attctgctct ccttcggtat cgacaccgtg caggcgatgg
     5221 caggcaaagt gcactggacg gtctatatcc tgcaaaccgg tttttccttc gcggtggcga
     5281 tcttcatcat cactcagggt gtgcgcatgt ttgtggcgga actctctgaa gcgtttaacg
     5341 gtatctccca gcgcctgatc cctggtgcgg ttctggcgat tgactgtgcg gctatttata
     5401 gcttcgctcc gaacgccgtg gtctggggct ttatgtgggg caccatcggt cagctgattg
     5461 cggttggcat cctggtcgcc tgcggttcct cgatcctgat tatcccaggc tttatcccga
     5521 tgttcttctc caacgccacc atcggcgtgt tcgctaacca cttcggcggc tggcgtgcgg
     5581 cgctgaagat ttgtctggtg atggggatga tcgaaatctt tggttgcgtc tgggtggtga
     5641 aacttaccgg tatgagtgcc tggatgggga tggcggactg gtcgattctg gcaccgccga
     5701 tgatgcaggg cttcttctcc atcggtatcg cctttatggc cgtcatcatt gtaattgcac
     5761 tggcttatat gttcttcgct ggccgcgcac tgcgcgcaga agaagatgca gaaaaacaat
     5821 tggcagaaca gtctgcttaa taaggagttt tgattatgac cgtacgtatt ctggctgtgt
     5881 gtggcaacgg acaaggcagt tccatgatca tgaagatgaa agtggaccag tttttaaccc
     5941 aatcaaacat tgaccacacg gtaaatagct gcgcggttgg cgaatacaaa agcgagttga
     6001 gcggcgcgga tatcatcatc gcttctacgc acattgcggg cgaaatcacc gtgaccggca
     6061 acaaatacgt ggttggcgtg cgcaacatgc tctctcctgc cgactttggc ccgaaactgc
     6121 tggaagtgat caaagcgcat ttcccgcagg atgtgaagta aggacgcgcc atgaaattac
     6181 atgattcgct ggcggagaat aaatccatcc gcctgcaggc tgaagctgag acatggcagg
     6241 acgcagtaaa aatcggcgtt gacctgctgg tggcggcgga tgttgtcgag ccgcgttact
     6301 accaggcgat tctggatgcg gttgaacagc acggtccgta ctttgtgctc gcaccaggcc
     6361 tggcaatgcc gcacggtcgc ccggaagagg gcgttaagaa aaccggtttt gcgctggtga
     6421 cgctgaaaaa gccgctggag ttcaaccacg aagataacga cccggtggat atcctcatca
     6481 ccatggcggc ggtcgatgcc aacacgcacc aggaagtggg catcatgcag atcgtcaacc
     6541 tgtttgaaga tgaagaaaat ttcgaccgtt tacgtgcctg ccgtaccgag caggaagtac
     6601 tggatctcat tgaccgcacc aacgcggcag cttaagaagg aattgaagat gtcattaccg
     6661 atgttgcaag tcgcgctgga caaccagact atggatagcg cctacgaaac cactcgcctg
     6721 attgccgaag aagtcgacat tatcgaagtg ggtaccattc tgtgcgtggg cgaaggcgtg
     6781 cgtgcggttc gcgatctgaa agcgctctac ccgcacaaaa tcgtgctggc agacgccaaa
     6841 attgccgatg cgggcaaaat tctttctcgt atgtgcttcg aagccaacgc tgactgggtg
     6901 accgtgattt gttgtgcgga tatcaacacc gccaaaggtg cgctggacgt agcaaaagag
     6961 tttaacggcg acgtgcagat cgaactgacc ggttactgga cctgggaaca ggcgcaacag
     7021 tggcgcgacg caggtattca gcaggtggtt tatcaccgca gccgtgacgc gcaggccgct
     7081 ggcgtggcgt ggggtgaagc cgatatcact gcgatcaaac gtctttccga tatgggcttc
     7141 aaagtcaccg tcaccggtgg cctggcgctg gaagatctgc cgctgttcaa aggcattcca
     7201 attcatgtgt ttatcgccgg tcgcagcatc cgtgatgccg cttctccggt tgaagccgcg
     7261 cgtcagttca aacgttccat cgccgaactg tggggctaag gagcggatat gttgtccaaa
     7321 caaatcccgc ttggcatcta tgaaaaagcg ctccccgccg gggagtgctg gctggaacgc
     7381 ctgcgactgg caaaaacgtt gggcttcgat tttgtcgaga tgtcggtaga tgaaactaac
     7441 gagcgcctgt cgcgccttga ctggagccgc gaacagcgtc tggcgctggt gaatgctatt
     7501 gttgaaaccg gcgtgcgtgt gccgtccatg tgcctttctg cccatcgtcg tttcccgttg
     7561 ggcagcgaag atgacgcggt gcgggcgcag gggctggaga ttatgcgtaa agccatccag
     7621 ttcgcccagg acgttggcat tcgcgtgatc cagctggcgg gctatgacgt ttactatcag
     7681 gaagccaata acgaaacgcg tcgtcgtttc cgtgacggcc tgaaagagag cgttgagatg
     7741 gcgagccgcg cgcaggtgac gctggcgatg gagatcatgg attatccgtt gatgaactcc
     7801 atcagcaagg cgctgggtta cgcgcactat ctcaacaacc cgtggttcca gctctacccg
     7861 gatatcggca acctgtcggc gtgggacaac gatgtgcaga tggagttgca ggccggaatc
     7921 gggcatatcg tcgcggtgca tgtgaaagac accaaaccgg gcgtcttcaa aaacgtgcca
     7981 tttggcgaag gtgtggtgga tttcgaacgt tgcttcgaaa cgctcaaaca gagtggctat
     8041 tgcgggccgt acctgattga gatgtggagc gaaacggcgg aagacccggc ggcagaagtg
     8101 gtgaaagcgt gtgattgggt gaaagcgcgc atggccaaag caggcatggt ggaggcggca
     8161 taatgcaaaa gctaaaacag caggtgtttg aagccaacat ggatctgccg cgctatgggc
     8221 tggtgacctt tacttggggc aacgtcagcg ctatcgaccg cgaacgcggg ctggtggtga
     8281 tcaagcccag cggcgttgcc tacgaaacca tgaaagcaga cgatatggtg gtggtcgata
     8341 tgagcggcaa ggtggtggaa ggggagtatc gcccatcttc cgacactgcg acgcatctcg
     8401 aactctaccg tcgttacccg tcgcttggcg gcattgtcca tacccactcc actcatgcca
     8461 ccgcatgggc gcaggcgggg ctggcgatcc cggcattagg caccacgcac gctgactact
     8521 tctttggtga tatcccgtgt acgcgtggat taagcgaaga agaggtgcag ggtgagtatg
     8581 aactgaacac tggcaaagtg attatcgaaa cgctgggcaa cgccgagccg ctgcatacgc
     8641 cgggaattgt ggtgtatcag cacggaccgt tcgcctgggg gaaagatgcc cacgacgcgg
     8701 tgcataacgc ggtagtgatg gaagaagtgg cgaaaatggc gtggattgcg cgcagtatta
     8761 atccacaact caatcatatc gacagctacc tgatgaataa acacttcatg cgtaagcacg
     8821 gtcctaacgc ttattacggg cagaagtaaa acacgcgctg cggaaatttc cttcctcggg
     8881 agataactgg tctaattccg cagccgtttt tcaaaaaaag ccccctgcga agggggcaaa
     8941 gcaaactatg gcaatgtttc gttggttata cctggtgcta gcgataaata tccgcgctgg
     9001 cgtgcatatt gccgttactc cccggttccc gcatcagaat tacgtggtag tacgttgcgc
     9061 cttgcgcatc tgtttcttca tttaatgcct gacgtgcttc gctttcagtg gcgaaattat
     9121 gattgatata aatcacgcct aagctttgca catcgtccat atttctggcc tggtggttat
     9181 taatttcaat ggctgcccat gtatttgcac ttagcaaaag cacagccaga agggctaaaa
     9241 ctcgactgaa catagatacc tcctcgacgg ctgactttgt gtgctctcct ctgtgatgat
     9301 cttctgattt aattttaatc aatgataaag aagttgatgg tgaccatttc tgatgcagtt
     9361 gttcaaaaaa acactgtgac ggaatgtgat gaacttcaaa tcagcggttt agaggttatt
     9421 tgcgaaaggg gagatttatt tctgctccgc ccttgagttt agcggggtat gccagtacta
     9481 taacgcgtca tttttcagcc gacctttaac acgttccttg cctccccggg attcggctga
     9541 cccagacagg aggctgaata atccgtaagg agcaattcga tgcgtcatta cgaaatcgtt
     9601 tttatggtcc atcctgacca gagcgaacag gttccgggca tgatcgagcg ctacactgct
     9661 gccatcactg gtgcagaagg caagatccac cgtctggaag actggggccg ccgtcagctg
     9721 gcttacccga tcaacaaact gcacaaagca cactacgttc tgatgaacgt tgaagctccg
     9781 caggaagtga tcgatgagct ggaaactacc ttccgcttca acgatgccgt tatccgcagc
     9841 atggttatgc gtaccaagca cgctgttacc gaagcatctc cgatggttaa agcgaaagac
     9901 gagcgccgtg agcgtcgcga tgatttcgca aacgaaaccg ctgatgatgc tgaagctggg
     9961 gattctgaag agtaatttct gatgaccaac cgtctggtgt tgtccggcac cgtgtgcagg
    10021 actccccttc gaaaggtcag tccatcagga attcctcact gccagttcgt gcttgagcat
    10081 cgttctgtgc aggaggaagc cggctttcac cggcaggcat ggtgtcaaat gcccgttatt
    10141 gttagcggac acgaaaacca ggccattact cacagtataa cggtcggcag tcgcataacc
    10201 gttcaggggt tcatttcatg ccacaaggca aagaacggac tgagcaaaat ggttttgcat
    10261 gccgagcaga ttgaattgat agattctgga gactagccat atggcacgtt atttccgtcg
    10321 tcgcaagttc tgccgtttca ccgcggaagg cgttcaagag atcgactata aagatatcgc
    10381 tacgctgaaa aactacatca ccgaaagcgg taagattgtc ccaagccgta tcaccggtac
    10441 ccgtgcaaaa taccagcgtc agctggctcg cgctatcaaa cgcgctcgct acctgtccct
    10501 gctgccgtac actgatcgcc atcagtaatc ggtcacggtc cattaatacg actttgagag
    10561 gataaggtaa tgcaagttat tctgcttgat aaagtagcaa acctgggtag cctgggtgat
    10621 caggtaaacg ttaaagcggg ctatgctcgt aacttcctgg taccgcaggg taaagctgtt
    10681 ccagctacca agaaaaacat tgaattcttc gaagcacgtc gcgctgaact ggaagctaaa
    10741 ctggctgaag ttctggcagc tgctaatgct cgcgctgaga aaatcaatgc actggaaact
    10801 gttaccatcg cgtctaaagc tggcgacgaa ggtaaactgt tcggttccat cggtactcgc
    10861 gacatcgctg acgctgtaac tgcagctggc gttgaagtgg ctaagagcga agttcgtctg
    10921 ccgaacggcg ttctgcgtac cactggcgaa cacgaagtga gcttccaggt tcacagcgaa
    10981 gtattcgcga aagtgatcgt aaacgtagta gctgaataat tcgttattca acgagacgta
    11041 aaaacgccga ccattggtcg gcgttttgct ttctattttt cgtcaggcat taatttcgca
    11101 agtagatcca ttcaccattc tcttgtgtcc ataagtcagg atggtttgct ggcgttagat
    11161 gcatacgatc actccttatg tctcggtcct gcggcactcg ctcgaaacct aaacccgagt
    11221 agattgcggc tgaatttgat aaggaacgta gaccaatttt caaatcatgg ggtaaatcag
    11281 aatacgcgtt tagcaatgca gcaatgatga atccgagggc attagaaacg cctgtaacag
    11341 tgtgaagttg gtcgatataa agatcgattg ttggtagttc agatgagcag caattaggca
    11401 tttgggcagg aagggttaca acgtatccta tcgtttggcc ggtatagtct ggagtactat
    11461 aaccaggtcc caaaacaacc tcgtgtattg tgtatttact gttgggttcg aaggattcga
    11521 catcttgcaa taaatatttc agttctgcgg ttaacctcgc acccgcgtaa gctttattgt
    11581 atttctgggc gtgaatatta tctaatgtag cttcatggtt ccatccaaaa gaattatcac
    11641 cattcattgt tactgaatcg ccacgggttt aacagacacc tcagagtcat ttaagatgg
//
LOCUS       DAJTRH010000110        11639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTRH010000110 DAJTRH010000000
VERSION     DAJTRH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            597..794
                     /locus_tag="OO339_003325"
     CDS             597..794
                     /locus_tag="OO339_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927839.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO339_003326"
     CDS             complement(744..1937)
                     /locus_tag="OO339_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5927840.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO339_003327"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO339_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5927841.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO339_003328"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO339_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5927842.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO339_003329"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO339_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5927843.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO339_003330"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO339_003330"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5927844.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO339_003331"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO339_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5927845.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10781..11117)
                     /locus_tag="OO339_003332"
                     /pseudo
     CDS             complement(10781..11117)
                     /locus_tag="OO339_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2951 a         2876 c         2983 g         2829 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgaa gaacttcttc agcacactgc taaaacgcat agtgatcgag
    10681 cgctgattct ggcgaacaac tgaactaata catcagaatc tgcattatgt taaataaata
    10741 tgaaaagatg gtttaaatac cccgttactt ttgacttaca ctatacggta tcgcatcgtt
    10801 taatattcgc accggccaga tttttatttc tattagttgt cacaatactg aatgcgtacg
    10861 accacagtat tctggctcct gtgtggttat gctttaattc tgcgttccgg gcagataagc
    10921 agttgcttgc aggaatcctt cttgtgttaa tgtcagttcc cctttttacc agtgctgatt
    10981 tccacattcc gtccaacaga gcttatagcc tttccctgga ttatagcatt gtccggctga
    11041 agttcttttt gaataataat agaagcactg ctggcagatc cagtccgttt ttcataaccc
    11101 actgtactga taaccataat ctaatcagta gaaattgagt cgaaaataag cactactcta
    11161 tacaggataa ttagaggtca gtttgattat tcacaattca tcatcagcat tttctatttc
    11221 tgacgaaatc aacatgaaaa taaccatata tgataattat tataataacg gctttaattg
    11281 gaatacatat attacaacgt attatatata attggtattc tgggaactat attctcaaaa
    11341 tacagtagaa acgaggtatg tttctggtgg aaaggacagt gggattaaaa agtaagaatt
    11401 gataaaaaaa acgccagcaa cacatagtgc cggcggaggg aataccccat ggagaaaatg
    11461 tgatgcctag agcatcatga atatataatt aaaaatagtt agcgttgtca atactttcac
    11521 aaaaataatt ttcgtagtat agaaagatat ttttatgcat gacctacctg aatttgctcc
    11581 gggtagaggt tataaataaa aattgaatca cgacaaacac aatattcata gtatggcga
//
LOCUS       DAJTRH010000111        11525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTRH010000111 DAJTRH010000000
VERSION     DAJTRH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(217..292)
                     /locus_tag="OO339_003333"
     tRNA            complement(217..292)
                     /locus_tag="OO339_003333"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
     gene            851..3103
                     /locus_tag="OO339_003334"
     CDS             851..3103
                     /locus_tag="OO339_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927846.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3439..4371
                     /gene="csiD"
                     /locus_tag="OO339_003335"
     CDS             3439..4371
                     /gene="csiD"
                     /locus_tag="OO339_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5927847.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4391..5659
                     /gene="lhgO"
                     /locus_tag="OO339_003336"
     CDS             4391..5659
                     /gene="lhgO"
                     /locus_tag="OO339_003336"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5927848.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5682..7130
                     /gene="gabD"
                     /locus_tag="OO339_003337"
                     /pseudo
     CDS             5682..7130
                     /gene="gabD"
                     /locus_tag="OO339_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7138..8424
                     /gene="gabT"
                     /locus_tag="OO339_003338"
                     /pseudo
     CDS             7138..8424
                     /gene="gabT"
                     /locus_tag="OO339_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8662..10062
                     /gene="gabP"
                     /locus_tag="OO339_003339"
     CDS             8662..10062
                     /gene="gabP"
                     /locus_tag="OO339_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5927849.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10083..10745
                     /gene="csiR"
                     /locus_tag="OO339_003340"
     CDS             10083..10745
                     /gene="csiR"
                     /locus_tag="OO339_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5927850.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="OO339_003341"
     CDS             complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="OO339_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5927851.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11279..11437)
                     /locus_tag="OO339_003342"
     CDS             complement(11279..11437)
                     /locus_tag="OO339_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5927852.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2962 a         2832 c         2874 g         2857 t
ORIGIN      
        1 tttgcccgct cggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaaacatt tttatgtttt cgtatacatc atattactct accattagag
      841 gaacgttatt atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac
      901 attgttatca tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata
      961 tctaaactgt ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga
     1021 cagagaaatt atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga
     1081 ttataaaatt tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag
     1141 tagcattaca gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac
     1201 aatggttgaa agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac
     1261 tcacattata tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat
     1321 tcctgactat gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca
     1381 gcaagaaaca tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa
     1441 atctcttact gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga
     1501 agcgattctt catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt
     1561 tgcacgttcc cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg
     1621 accagaaact cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct
     1681 ttgtaccatc cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc
     1741 taaaaaattt ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg
     1801 aagtggtaaa aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga
     1861 tatagaaagt ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga
     1921 tccagtaaca aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga
     1981 cttcgaagca agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa
     2041 agaaaagagt ttaaatggaa tgcctaaaac aacccccaaa agtgaccagg ttattatgtt
     2101 gcaaaatcaa tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc
     2161 agcaaaacac tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta
     2221 taatgagcga ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat
     2281 gaattacatg gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct
     2341 ttatgaaaga gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc
     2401 cttttcattc gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag
     2461 catcccatca gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa
     2521 tcttttcaat gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat
     2581 ggccgctctc ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac
     2641 gactgaaatt aaaaattatg atgggcccac gcgtgtcggt ggtgaggcgt ggcttaataa
     2701 aggttgctct acctatcagc agctctacaa tgaattccac gcattatata tagataaagc
     2761 aggaatatgg agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg
     2821 tggggattct gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg
     2881 aaacgaagaa gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat
     2941 ggggagtaat agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa
     3001 agtatttacg cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct
     3061 tttagggaaa acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc
     3121 cccgataaaa cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc
     3181 atttactgtt ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt
     3241 tgctttagtt attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta
     3301 atttgttgct tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga
     3361 aatgtagata tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc
     3421 ataagaggat cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca
     3481 ggccaggact atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa
     3541 ctcaccttca ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg
     3601 caggcgctgg agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg
     3661 tgtgcgaatc agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg
     3721 ctgttgatca atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg
     3781 gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat
     3841 tacgcgcgtt tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac
     3901 cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg
     3961 atgatgaaaa tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt
     4021 cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca
     4081 gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag
     4141 ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc
     4201 aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc
     4261 tggctgcacg gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt
     4321 cagcgtggct atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg
     4381 aattgagcgg atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc
     4441 catgcaactg attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg
     4501 cccggcctgt caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac
     4561 gcccggcagc ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg
     4621 cgatcaaaac ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct
     4681 cgaaatggaa cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga
     4741 gtggataaac gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat
     4801 ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat
     4861 cttccaggcc agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca
     4921 taaaaacggc gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat
     4981 tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt
     5041 tattatctgc ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt
     5101 taaccacctg atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac
     5161 ccgcatgatc gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga
     5221 aggctatcgc aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg
     5281 gattcgccgg gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct
     5341 gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga
     5401 tctccagccc tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat
     5461 tgacgatttt ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc
     5521 ggcagcgaca tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt
     5581 ggcaagccag agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca
     5641 cgccgcattt aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta
     5701 acttattccg ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag
     5761 tcatcgacgt caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg
     5821 cagatgaaac ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc
     5881 tcaccgccaa agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc
     5941 aggacgattt agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag
     6001 gcgaaatcag ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt
     6061 atggcgacac cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga
     6121 ttggcgtcac cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag
     6181 ccggtccggc gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt
     6241 tctctgcgct ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta
     6301 acgtggtcac cggttcggca ggcgtggtcg gtaatgaact gaccagcaac ccgctggtac
     6361 gcaaactgtc gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga
     6421 aagacatcaa aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg
     6481 atgccgatct cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc
     6541 aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg
     6601 aaaaattaca gcaggcgggg agcaaactgc acatcggcga cgggctggag aaaggcgtca
     6661 ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg
     6721 cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact
     6781 tcttccagcc aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga
     6841 cgttcggccc cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag
     6901 ccaatgacac cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct
     6961 tccgcgtcgg cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca
     7021 atgaagtggc cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt
     7081 atggcatcga agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg
     7141 cgaaggaaca gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt
     7201 gggcaaattc acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgtttaa
     7261 ggccgtgagt atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat
     7321 ccgaaggtgg tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag
     7381 gtgctggctt acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc
     7441 gatttcgcca agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta
     7501 aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac
     7561 gggcgcacgc attacacgct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg
     7621 gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc
     7681 gaggatgacg ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat
     7741 atcgccgcca tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca
     7801 gcctttatgc agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat
     7861 gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg
     7921 gcgccggatc ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc
     7981 accgggcgcg cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc
     8041 ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat
     8101 ctgctgcaaa aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc
     8161 gaaaaacacc cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg
     8221 tttgaagatg gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc
     8281 gcccgcgata agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc
     8341 cttgtaccac tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag
     8401 tgttttgatg aggcgaagca gtagcaccgt ccccatgcag gaggcgacgc tgcgcgtctt
     8461 gtccggccta cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac
     8521 gtgaacagga ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa
     8581 tcaataacaa ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac
     8641 acactttcga gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg
     8701 ctgaaatcac gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt
     8761 gtcggttcca gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc
     8821 gccggattac tggtggttat gattatgcgg atgctggcgg aaatggcagt tgccacgccc
     8881 gataccggtt cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc
     8941 atcggctggt tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc
     9001 gccatgatcc tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt
     9061 accctcgcct taactggcag taatttatta agcgttaaaa actacggcga atttgagttc
     9121 tggctggcgc tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca
     9181 attagcggtt tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc
     9241 ggctttatgc ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg
     9301 tttatgggcg cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat
     9361 attgtccgcg ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt
     9421 tttgtcgtcg tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat
     9481 cgctcggttc tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata
     9541 ttactttccg taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac
     9601 tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc
     9661 ccgtacgtgg cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat
     9721 tacgcgcctg cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg
     9781 gtttatttag tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga
     9841 agcgaaattc gcttgcgaat gtggcttgat ccctggctca cctggctggt catcggcttt
     9901 attacctttg tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc
     9961 accggcttat tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag
    10021 ctggtattgt ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca
    10081 ccatgaccat tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta
    10141 attttcaacc ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg
    10201 ttgggccgtt gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga
    10261 atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg
    10321 cccgcgccaa tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat
    10381 gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca
    10441 gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg
    10501 cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc
    10561 gttatcgatt tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac
    10621 acgatcagca ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg
    10681 agttaatgcg ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca
    10741 attaactact cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt
    10801 agcatcggtt tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt
    10861 ttggaaatgg cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct
    10921 ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca
    10981 acaaggattt tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg
    11041 tcggcaattt gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc
    11101 tgcaccttct tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt
    11161 tctccggcat ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac
    11221 actcagaaag tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt
    11281 aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca
    11341 gcagaatatt aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg
    11401 gcagaatgat ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta
    11461 ctcattgttt aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag
    11521 tttca
//
LOCUS       DAJTRH010000112        11372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTRH010000112 DAJTRH010000000
VERSION     DAJTRH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(89..733)
                     /gene="cheZ"
                     /locus_tag="OO339_003343"
     CDS             complement(89..733)
                     /gene="cheZ"
                     /locus_tag="OO339_003343"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5927853.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLMHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(744..1133)
                     /gene="cheY"
                     /locus_tag="OO339_003344"
     CDS             complement(744..1133)
                     /gene="cheY"
                     /locus_tag="OO339_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5927854.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1148..2197)
                     /gene="cheB"
                     /locus_tag="OO339_003345"
     CDS             complement(1148..2197)
                     /gene="cheB"
                     /locus_tag="OO339_003345"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5927855.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2200..3060)
                     /gene="cheR"
                     /locus_tag="OO339_003346"
     CDS             complement(2200..3060)
                     /gene="cheR"
                     /locus_tag="OO339_003346"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5927856.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3079..4680)
                     /gene="tap"
                     /locus_tag="OO339_003347"
     CDS             complement(3079..4680)
                     /gene="tap"
                     /locus_tag="OO339_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5927857.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4726..6387)
                     /gene="tar"
                     /locus_tag="OO339_003348"
     CDS             complement(4726..6387)
                     /gene="tar"
                     /locus_tag="OO339_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5927858.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6532..7035)
                     /gene="cheW"
                     /locus_tag="OO339_003349"
     CDS             complement(6532..7035)
                     /gene="cheW"
                     /locus_tag="OO339_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5927859.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7056..9020)
                     /gene="cheA"
                     /locus_tag="OO339_003350"
     CDS             complement(7056..9020)
                     /gene="cheA"
                     /locus_tag="OO339_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5927860.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9025..9951)
                     /gene="motB"
                     /locus_tag="OO339_003351"
     CDS             complement(9025..9951)
                     /gene="motB"
                     /locus_tag="OO339_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5927861.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(9948..10835)
                     /gene="motA"
                     /locus_tag="OO339_003352"
     CDS             complement(9948..10835)
                     /gene="motA"
                     /locus_tag="OO339_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5927862.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(10962..>11372)
                     /gene="flhC"
                     /locus_tag="OO339_003353"
     CDS             complement(10962..>11372)
                     /gene="flhC"
                     /locus_tag="OO339_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5927863.1"
                     /translation="LPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYR
                     LYLEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACS
                     LCQPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
BASE COUNT         2585 a         3215 c         2778 g         2794 t
ORIGIN      
        1 accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct tggcatcatc
       61 gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac aaatcgtcca
      121 cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt ccattaagca
      181 aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc aacagcacca
      241 tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata acctgcccgg
      301 tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta gtaaagctgg
      361 tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca cgggcgtcgg
      421 caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct tttgctgatt
      481 tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc cgctccgcag
      541 cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg atggcttccg
      601 ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc agcatatgca
      661 tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca ggtttgattg
      721 atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt gttgagtttt
      781 tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc cgcttgcgcc
      841 gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg caatgccgac
      901 atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt gggcatgttc
      961 cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc gtcgacacca
     1021 tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg cactatgcgt
     1081 cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc catttcacac
     1141 tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt tgctggctta
     1201 cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg cgcggcatgc
     1261 cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc tgacgcatcg
     1321 ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca accgcattac
     1381 gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga tgacggttaa
     1441 ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc agttccatat
     1501 gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg tcttcggctt
     1561 ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg gtgaaaccgg
     1621 gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt tgcagtacgt
     1681 gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt tcagaactca
     1741 acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct gcaaggctcg
     1801 cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc attccttcgc
     1861 gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc gcgcgcagcg
     1921 tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt ggacgcaaac
     1981 gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca tccagcgtca
     2041 gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca ggcgcggtcg
     2101 ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg cgcatcagtg
     2161 ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac ttagcgcata
     2221 caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt cagagtgacc
     2281 cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa tctcctgctg
     2341 ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa acggccccgg
     2401 cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt tcgccagctc
     2461 ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat accgttgcag
     2521 ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc tggctttttc
     2581 cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca cggtgcccaa
     2641 tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag ccgccgcgct
     2701 ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag ggaaatgatg
     2761 agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg cctgccactc
     2821 accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg tcagtcccag
     2881 agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt cagccagaac
     2941 gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt cggaaagcgc
     3001 caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag atgaagtcat
     3061 agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg ccgactcatg
     3121 tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa gatggtcggc
     3181 ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg agttctgctg
     3241 agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc gccgttgttc
     3301 ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg aactgacaat
     3361 atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga cacgattgac
     3421 tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc tggcaagatt
     3481 gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc gagccgcttc
     3541 cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga taacgctgat
     3601 aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag tcatggtact
     3661 gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt ccgacgcctg
     3721 tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac tggcggccgt
     3781 ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat tgcctgcgac
     3841 aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac tcaccgtccc
     3901 acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct cattacgacc
     3961 atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat ggctaccgat
     4021 aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc tgatgtagat
     4081 cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt tacgctggct
     4141 ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct caaagttaac
     4201 gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa gttgattact
     4261 ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac ttttctccgt
     4321 ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa aacttttaaa
     4381 cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt taatgtcatc
     4441 cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac tggcctgtaa
     4501 cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct cgccgtgatt
     4561 cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc caatctgcaa
     4621 gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac gattaaacat
     4681 caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa atgtttccca
     4741 gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct cactggcagg
     4801 acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga acgcggaaac
     4861 cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag ctgattcctg
     4921 caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca atgcgacttg
     4981 atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa tgtcagtcac
     5041 gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga ccagcaccga
     5101 accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt ttgccgccag
     5161 ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac cacggccctg
     5221 ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag tctggaaggc
     5281 aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg cgatctcatg
     5341 catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt cggaggcact
     5401 ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg tcgcggtgag
     5461 ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag tacgggagga
     5521 aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat ctgaaccttc
     5581 gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc tctgcgccag
     5641 gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac caccggcgat
     5701 ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc gaatgccgta
     5761 ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt gccactgggc
     5821 aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt cactgctgag
     5881 ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg gctgagcgaa
     5941 ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg ctgtgtaata
     6001 gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta acggtgccat
     6061 gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg cgctatcgag
     6121 caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc gcaccgctga
     6181 acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca gctcgccctg
     6241 ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa gggaagaaaa
     6301 aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca ttaccaacag
     6361 cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt tgatctcgtt
     6421 atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat tgaaataaaa
     6481 aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga attacgccac
     6541 ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt tttcgatgtt
     6601 caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt ctgttgaaag
     6661 cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa gcacgtctga
     6721 aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga taactaccgt
     6781 gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa caatcggcac
     6841 aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg caatccgtgt
     6901 tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt actcttcatc
     6961 accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct ttgttacatt
     7021 cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt cgccatacgt
     7081 tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac gctgccgtcg
     7141 ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc aaggttttta
     7201 accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg gcgaccgcca
     7261 ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc gacgttgaac
     7321 actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac ccgctcgccg
     7381 ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac agcattcagc
     7441 ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat ggccagcgtc
     7501 agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat ttcgacatga
     7561 ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg cccggagacg
     7621 tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc aacttcgtcg
     7681 tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag aattcgctca
     7741 cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc ctgatgttcg
     7801 gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt ttctggcagt
     7861 tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat aatgcgttct
     7921 atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac ctgcttgccg
     7981 agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata ttccatcggc
     8041 atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg taactgcccc
     8101 atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg ctgggcaagc
     8161 atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac cttttctacc
     8221 gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc cacgcggccg
     8281 gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt tggcgaaact
     8341 tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac cgctgtgatg
     8401 tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc tttcaccacg
     8461 tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc gccggccttc
     8521 aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc ttgcggttca
     8581 cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt cgcttctaat
     8641 gccagttgac gcaaggcctg gcagatataa tcgtagctgg ctgcatccgg ctcttgcgac
     8701 tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa cagattgata
     8761 atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt ttccatcaga
     8821 tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt gatcgagtgg
     8881 gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg caaaaccagc
     8941 agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt ctgataaaaa
     9001 tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg tgggaacact
     9061 gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct ggctttcggc
     9121 gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc tgatgcgacg
     9181 gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca tgccgacgac
     9241 acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg atgcattggc
     9301 ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt agggaaaatc
     9361 atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag gcgcgatggc
     9421 gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa acatcgggcg
     9481 attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga ttttgagatg
     9541 gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat caccccgcaa
     9601 tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg gctgcttatt
     9661 cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc tttcactatt
     9721 ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt actccgcaat
     9781 ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa aaaaggccat
     9841 catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg ccccgtggct
     9901 tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca tgcttcctcg
     9961 gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag ttcaataaac
    10021 gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat aggcggtgcg
    10081 taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt gctggtttcg
    10141 gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc caataaaata
    10201 ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc ggcaggacga
    10261 tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc aaacgccgga
    10321 agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct ttcgtgcgtc
    10381 tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc gctgataatc
    10441 aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat gcgtgggtag
    10501 ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga aaacatcccc
    10561 atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat atacattgct
    10621 ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc tttaatcgct
    10681 ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat caccagttca
    10741 gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc gaaaactgta
    10801 ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca ctgttgacca
    10861 tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa agcagcggta
    10921 cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc ctgtactctc
    10981 tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt acgtcttttt
    11041 actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg ctggtgagcg
    11101 tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa tccactttca
    11161 acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc ttctgcttgt
    11221 gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc attacacaaa
    11281 ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac gttttgttcc
    11341 caggtcataa accagtcggt tgagaatggc ag
//
LOCUS       DAJTRH010000113        11322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTRH010000113 DAJTRH010000000
VERSION     DAJTRH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..148)
                     /locus_tag="OO339_003354"
     CDS             complement(<1..148)
                     /locus_tag="OO339_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5927864.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKH"
     gene            524..2164
                     /gene="trg"
                     /locus_tag="OO339_003355"
     CDS             524..2164
                     /gene="trg"
                     /locus_tag="OO339_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5927865.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2202..3125)
                     /locus_tag="OO339_003356"
     CDS             complement(2202..3125)
                     /locus_tag="OO339_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5927866.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3342..4685
                     /locus_tag="OO339_003357"
     CDS             3342..4685
                     /locus_tag="OO339_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5927867.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4910..6565
                     /gene="opgD"
                     /locus_tag="OO339_003358"
                     /gene_synonym="mdoD"
     CDS             4910..6565
                     /gene="opgD"
                     /locus_tag="OO339_003358"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5927868.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6705..6929
                     /locus_tag="OO339_003359"
     CDS             6705..6929
                     /locus_tag="OO339_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5927869.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6992..7528
                     /gene="rimL"
                     /locus_tag="OO339_003360"
     CDS             6992..7528
                     /gene="rimL"
                     /locus_tag="OO339_003360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5927870.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7523..8503)
                     /locus_tag="OO339_003361"
     CDS             complement(7523..8503)
                     /locus_tag="OO339_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927871.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8627..9619
                     /gene="tehA"
                     /locus_tag="OO339_003362"
     CDS             8627..9619
                     /gene="tehA"
                     /locus_tag="OO339_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5927872.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9616..10209
                     /gene="tehB"
                     /locus_tag="OO339_003363"
     CDS             9616..10209
                     /gene="tehB"
                     /locus_tag="OO339_003363"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5927873.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10512..11168
                     /locus_tag="OO339_003364"
     CDS             10512..11168
                     /locus_tag="OO339_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5927874.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11186..>11322)
                     /locus_tag="OO339_003365"
     CDS             complement(11186..>11322)
                     /locus_tag="OO339_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5927875.1"
                     /translation="GGAPISVIRQYIEQQQTPGQVENRALYPRPEGWGFTAHRIKSAI
                     "
BASE COUNT         2919 a         2643 c         2852 g         2908 t
ORIGIN      
        1 catgtttttc atagagggca acccatgtcc tgacctgggt tcgggggaca ccaaaacgtg
       61 ccgagatgat cctgtaacca tcatcagtag tgaagtagtg attcacgact tcaaggcgct
      121 tttcaaaagg gtattttggc tttgacatat taggggctat tccatttcat cgtccaacaa
      181 aatgggtgca gtacagttga tagaaatatc ggtcggggcc ttcgtctttc tgattcccgg
      241 ttagcctgaa aacagaaagt ctcaggcacc cgcaggcatc ctatgaggtt tccttaggga
      301 cgaaaataat cacttcacga aattgcgtgc tgttttccag aatttttcgt cattcgggtt
      361 agccagttta gccattcgtt actctcttca ttccaatagc attaattttc tctgcaataa
      421 ttgttgtaaa aatgtgacgc aaagaggttt ttggtcataa gtaattaccg tcaagtgccg
      481 atgactttct atcaggagta aacctggacg agagacaacg gtaatgaata caactccctc
      541 acagcgatta ggttttttgc atcacatcag gctggttccg ttatttgcct gcattcttgg
      601 cggtatctta gttctattcg cattaagttc tgctctggct ggctatttcc tctggcaggc
      661 cgatcgcgat cagcgtgatg ttactgcgga gattgagatt cggaccgggt tagcgaacag
      721 ttcagatttt ttgcgttcag cccggatcaa tatgattcag gccggggctg cgagtcgtat
      781 tgcggaaatg gaagcaatga agcgaaatat tgcgcaagcc gaatcggaga ttaaacagtc
      841 gcagcaaggt tatcgtgctt atcagaatcg accggtgaaa acacctgctg atgaagcctt
      901 cgacactgaa ttaaatcaac gctttcaggc ttatatcacg ggtatgcaac ctatgttgaa
      961 atatgccaaa aatggcatgt ttgaagcgat tatcaatcat gaaagtgagc agatccgacc
     1021 gctggataat gcttataccg atattttgaa caaagccgtt aagatacgta gcaccagagc
     1081 caaccaactg gcggaactgg cccatcagcg cacccgcctg ggtgggatgt tcatgattgg
     1141 cgcgtttgtg cttgccctgg tcatgacgct gataacattt atggtgctac gtcggatcgt
     1201 cattcgtcca ctgcaacatg ccgcacaacg gattgaaaaa atcgccagtg gcgatctgac
     1261 gatgaatgat gaaccggcgg gtcgtaatga aatcggtcgc ttaagtcgtc atttacagca
     1321 aatgcagcat tcactgggga tgacagtagg gactgttcga cagggcgcgg aagagattta
     1381 tcgtggcacc agcgaaattt cagctggcaa tgcggacctg tcatctcgca ccgaagaaca
     1441 agcggcggct atcgaacaaa ctgccgccag catggagcaa ctcactgcga cggtgaaaca
     1501 gaatgcggat aacgcgcatc atgccagcaa actggcgcaa gaggcttcta ttaaagccag
     1561 cgatggcggg cagacggttt ccggtgtagt aaaaacgatg ggcgctatct ccacgagttc
     1621 gaagaaaatt tctgagatca ccgccgtcat caacagtatt gctttccaga cgaatattct
     1681 ggcactgaat gctgccgttg aagccgcgcg agcgggtgag caagggcgtg gatttgccgt
     1741 tgtcgccagc gaagtacgga cactcgcaag ccgcagcgct caggcagcga aagagattga
     1801 aggcttgatc agtgaatcag tcaggttaat tgacctgggg tcggatgagg tggcaacggc
     1861 agggaaaacc atgagcacta ttgttgatgc cgtcgcgagt gtcacacata tcatgcagga
     1921 aatcgccgcc gcctcggatg aacaaagtag aggcataact caggttagcc aggcgatttc
     1981 tgaaatggat aaggtgacgc aacagaatgc ttctctggta gaagaggcct cagcagcggc
     2041 ggtgtccctt gaagaacagg ctgcacgatt aactgaggcg gtgaacgtat tccgtctgaa
     2101 caaacattct gtgtcggcag aacctcgcgg agcgggtgaa ccagttagtt tcgctacggt
     2161 gtgaaaatgt tcaaggaggg atcgacagat cccttcacct ttcagaacgg cattgatttt
     2221 cgaatagcgt taatcatcaa ctggcaacca gaagagaacg tcgcatctac gcgggtcagt
     2281 attccaatcg gttcgcctgc accatgtccc ggaacaggca gggccaccag cgtggcatga
     2341 cgcaggtcgt cttttacagc gccagaaggg acaaaccaca cgtaatcgta ttcaacggta
     2401 agttgacgag atagcgaagt agacagcgtt tcgatacaac ccgaaggaat tttacagccc
     2461 tggctctgca ctaatgcatc tgaatgctgg cgtggcgcag tgccttctgg tgatacaacg
     2521 accggccatt ccagcacccg gcttagcgtt acgttctcct gaagtagcgg gtgattaggg
     2581 cggacaacca gcttcaacga ttcaagaaac agcagttcgt aattaagccc ggtcatcagt
     2641 tcaggatctg acatccgacc aatgccgata tcgatttccc cggtttttaa acccgccaga
     2701 atcatagggt tactcattgt cgcaacttgc aaggtcgtct ctttttgttg ctgatgaaac
     2761 tgacctataa ccgaaggtaa tatccccagt gccgcagtag gtagtgcacc aaccctgacg
     2821 acatcattat taagaccttc tttacgatga agcgactgtc cggcagtgtt gatggcgtca
     2881 agaactctga ctgcatgcgt taaaaattgt tcgccgggta aggtaagttg cgccccctga
     2941 cgaccacgct caaacaagcg agcgcccgtc agctgctcca gttcattcaa tgtattagag
     3001 agcgcaggtt gactcaaatt aagggtttca gctgcgcgcc ccaaagttcc ttgttgtgcg
     3061 acagctacga atgtatgaag gtggcgcaaa cggatgcgct gactaaacag actatttttt
     3121 tccataagcg atgttaaaaa cgaagcggtg tcgctgacaa gtgaagttgt ttgattatga
     3181 taacttgatt gcaaaatatt attaacaatt aaagcaatta tgttacagca aaatggataa
     3241 tattgatgtt ttcgcggcga gatcacagtt tgtaaattct tcccgccaga gtgaatgcgg
     3301 ttacctacac tccagattac tgaccactgg aggcagacac tatggcgaac agcatcacgg
     3361 cggatgagat tcgggaacag ttttcgcagg caatgtcagc catgtaccag caagaagttc
     3421 cgcaatatgg cacgctgctg gaactggtag ctgatgtgaa tctggctgtg ctggaaaaca
     3481 atcctcaact gcacgaaaaa atggtaaatg cagacgagct ggcgcgactg aatgttgaac
     3541 gtcatggggc gattcgcgtt gggactgcac aagagcttgc tactcttcgg cggatgtttg
     3601 ccattatggg gatgtacccg gtgagctatt acgatctctc gcaggcaggg gtgccggtac
     3661 attcgacagc atttcggccc attgatgatg cttctctggc gcgtaatccc ttccgcgttt
     3721 ttacatcctt actccgcctt gagcttatcg agaacgaaat tttgcgccag aaagcggcgg
     3781 agattctgcg tcagcgcgat atcttcaccc cacgttgtcg acaactgtta gaggaatatg
     3841 agcagcaggg cggttttaac gaaacacagg cacaggagtt tgtgcaggaa gccctggaaa
     3901 cgtttcgctg gcaccagtta gcaacggtag atgaagaaac ctatcgcgcc ttgcacaacg
     3961 aacatcggtt gattgctgat gtggtctgtt ttcctggatg ccatatcaac cacctgacgc
     4021 cacgtacgct ggatattgac cgggtgcagt cgatgatgcc tgaatgcgga attgaaccca
     4081 aaattctcat cgaggggccg ccgcgccgcg aggtaccgat tttactacgc cagaccagct
     4141 ttaaagcact ggaagagacg gtgttgtttg cggggcagaa acagggcacg cataccgcgc
     4201 gctttggtga aattgagcag cgtggcgtgg cattaacgcc gaaagggcga caactgtatg
     4261 atgatcttct gcgtaacgct ggaaccgggc aggataatct cactcaccaa atgcatttac
     4321 aggaaacctt ccgcactttt cctgacagtg agtttttaat gcgtcagcaa gggttggcat
     4381 ggttccggta ccgtctgacg ccttcgggtg aggcgcatcg tcaggcgatt catcctggag
     4441 acgatccaca gcccttaatt gaacgtggtt gggtagtggc gcaacccatc acctatgaag
     4501 atttcttgcc cgttagcgcg gcggggatct tccagtcaaa tctgggtaat gaaacgcaga
     4561 cacgcagtca cggtaatgcc agtcgcgaag catttgagca ggcgttgggt tgtccggttt
     4621 tggatgagtt ccagctttac caggaagcgg aagaacgcag taaacgtcgc tgtggtttgc
     4681 tttaaattct gaccatccgc ctttgcaaaa atttgcctga ttttacaaac gaatcaggct
     4741 catcccatcg acataaaaaa aatgccgatt tatgcatatt ctctcagttc aacaattgga
     4801 ttattaataa atattgtcta gagtgagcgg tcataaataa gcactttctt gccgctgaaa
     4861 acgaccagcg cgggaccatt cacaacacca gaaggactca ctttcaggta tggatcgtag
     4921 acgatttatt aaaggttcaa tggctatggc cgccgtgtgc ggtaccagcg gcattgcttc
     4981 tcttttttct caggcagcat tcgcggcaga ttctgatatt gccgacgggc aaacccagcg
     5041 ttttgacttc tccattctac agtcaatggc gcacgactta gcgcaaacag cgtggcgtgg
     5101 tgcgcctcgt ccgttacctg acacgctggc gacaatgacg ccgcaggctt ataacagtat
     5161 tcaatacgac gccgaaaaat cgctctggca taacgttgag aaccgtcaac tggacgctca
     5221 gttcttccat atgggaatgg gattccgtcg ccgcgttcgt atgttttctg tagatccagc
     5281 aacacatctg gcgcgtgaaa ttcactttcg cccggagttg ttcaaataca acgatgcagg
     5341 tgttgatacc aaacaattag aagggcaaag cgatctcggc tttgccggtt ttcgcgtgtt
     5401 taaagccccc gaactggcgc gccgtgatgt agtatcattt ctcggcgcga gttatttccg
     5461 cgccgttgat gatacatatc aatacggttt gtcggcccgc ggcctggcga tcgacactta
     5521 caccgacagt aaagaagagt tccccgactt taccgccttc tggtttgata cggtaaaacc
     5581 gggggcaact acctttaccg tttatgcgtt gctcgatagc gccagcatta ctggtgccta
     5641 taagttcact atccattgtg agaaaagtca ggtgattatg gatgtggaaa atcacctgta
     5701 tgcgcgcaaa gacattaaac agctgggcat ttcgccgatg accagtatgt tcagctgcgg
     5761 tactaatgaa cgtcggatgt gcgatacaat tcatccgcaa attcatgact ctgatcgtct
     5821 gtccatgtgg cggggcaacg gcgagtggat ttgccgtccg ctgaataatc cgcaaaaatt
     5881 gcagttcaat gcttacaccg acaacaaccc gaaagggttt ggtttattgc aactggatcg
     5941 tgacttctcc cattatcagg acattatggg ctggtataac aaacgcccaa gtctgtgggt
     6001 ggaaccgcgt aacaagtggg gtaagggcac catcggcctg atggaaatcc caacaacggg
     6061 cgaaacgctg gataacattg tctgcttctg gcagccagaa aaagctgtaa aagcaggtga
     6121 tgagtttgca ttccagtatc gtctgtactg gagtgcgcaa ccgcctgttc attgcccatt
     6181 agcgcgcgtt atggcgacgc gtaccggcat gggcggtttc ccggaaggtt gggcgccagg
     6241 tgaacactat cccgaaaaat gggcgcgtcg ttttgccgtc gatttcgttg gtggtgatct
     6301 gaaagctgcc gcgccaaaag gcattgagcc ggtgattacg ctttccagtg gggaagcgaa
     6361 gcaaatcgaa attctctata ttgaacccat cgatggttat cgtattcagt ttgactggta
     6421 tccgacttcg gactccactg atccggtcga tatgcggatg tatctacgtt gtcaggggga
     6481 cgctatcagt gaaacatggc tgtatcagta tttcccgcca gcgccggata aacgtcagta
     6541 tgttgacgac cgcgtgatga gttaatcgtt ttttcttcgg caccttcttc gggaggtgcc
     6601 gtctggttaa acacgatccc gctcgcattt ttccctaagt taaatgagta atctgatggt
     6661 gtgtatttca gatacaccgt gtcagccact aacagggagt gcgtatgttt ccagaatacc
     6721 gagatttaat atcccgtctg aaaaacgaaa atcctcgctt tatgtccttg ttcgataaac
     6781 acaataaact tgatcatgaa attgccagaa aggaaggttc cgacggtcga gggtacaatg
     6841 cggaagtggt ccgcatgaaa aaacaaaagc tacagttaaa agatgagatg ctcaaaatcc
     6901 tgcagcagga gagcgtcaaa gaggtgtaaa ctttcctaag ccgccagcca ggcggctttt
     6961 ttaacaacag catggattga ctggagataa gatgactgaa acgataaaag taagcgaatc
     7021 acttgaatta catgctgttg cggaaagtca cgtcacacct ctttatcagt taatctgtaa
     7081 aaataaaacc tggttacagc agtcgctaaa ctggccgcag ttcgttcaga gtgaagagga
     7141 cacgcgaaaa acggtgcagg gtaatgtgat gttgcatcaa cgcggctatg ccaaaatgtt
     7201 catgattttc aaagaagatg aacttatcgg cgttatctcg tttaatcgta ttgaaccact
     7261 gaataaaact gctgaaatcg gctactggct ggacgaatct catcaggggc aggggatcat
     7321 ttctcaggcg ctgcagacat tgattcatca ttacgctcag tctggtgaac ttagacgctt
     7381 cgtgatcaaa tgtcgggtgg acaatccgca aagcaaccag gtcgctttgc gcaatggttt
     7441 tatccttgaa ggttgcctga aacaggctga gttcctgaat gatgcctatg atgatgtgaa
     7501 cttatacgcg cgtattatcg attcataata accctgccag cggcgttcgc gtaatgcgct
     7561 cctcgccgtt aatgactttt ggaccacgga catgaaccgt gtcgccatca aaggcttcaa
     7621 cgacggcgtg gtcggttaac tccacatggt tttcaattat caccgcacct gtaatacggc
     7681 tctcaccttg tattacaacg tgctcgtcga gcagaatcgg tccaccacgt accacggcat
     7741 ttccgccaat caagacatga tgttttaaca cacaattacc ttccacaatg gcatattccg
     7801 ccacctgcga actgtaatga atcgtcggaa tggcatcttc ttctatgcca gctttcacct
     7861 gcgcgtgacc gtagacttta gcgcaatcgc atagccagac gttgttctct tcattacctt
     7921 caacactggc aaaatcaaaa acttcggcgc gatgttcaat aaaagcatac cggacgacgg
     7981 catcgccata aatttgtgcc tggtggacaa tacgcgaggc gctaaccctt gcgcgatcat
     8041 aaatttggag caggagttga tggtcgggcg ttaagccttg tgcggcgacg atcatagaat
     8101 gttgatcaat taaggcgtga ccaaatattc tacattgcac gtaaactaat gagtcatgaa
     8161 tggtgacact gtcacttata tatgcaccct ggctgatttc actattatcg atccagacgt
     8221 tatccgttgc gtaaacctct ccccacagca cgctggtgcc ggtgatacgc gtattaccgg
     8281 aaatcaccgt tccgccgaac gcaatagcat tctggtcgta aatccagcaa tttccttcct
     8341 gtgctaacac cgtttcgcga tcgatccagc cgccagcggt tcctgctatc acatcgttaa
     8401 aatcgctaat ggcgataatc tgccgtaata acacactttt tttagtgcca tcttcctgat
     8461 aactaaaggc tcgctgttct tcacttaggc gatatttgcg catagcgttt tcccacaggt
     8521 gacttactat aaccgtagca aattctgcgg ctctggctat gctcgagaaa attccataaa
     8581 atgcatttca aatatacttt ataaattaaa caaaatgagt aagaagatgc agagcgataa
     8641 agtgctcaat ttgccggcag gctactttgg tattgtgttg gggacgatag ggatgggatt
     8701 tgcctggcgc tatgccagcc aggtttggca ggtcagccac tggttagggg atgggctggt
     8761 gattctggcg atgatcatct ggggattatt gactagcgca tttattaccc gactcatacg
     8821 ctttccgcat agcgtgctgg cggaagttcg ccatccagtg ctgagcagtt ttgtgagttt
     8881 gtttccggca acgacgatgc tggtggcgat tggttttgtt ccgtggtttc gcccactggc
     8941 ggtgtgcatg ttcagttttg gtgtcgtggt tcagttggct tatgccgcct ggcaaactgc
     9001 gggattatgg cgcggatctc accctgaaga agctaccacg cctggactgt atctgccgac
     9061 agttgccaac aactttatca gcgcaatggc ctgtggtgcg ttgggctaca ccgacgccgg
     9121 tctggtgttt ttaggcgcag gcgttttctc atggctaagc cttgaaccgg tgatcttgca
     9181 gcgtctgcgc agttcgggag aattacccac ggcactgcgg acatcactcg gcattcagct
     9241 cgctcctgcg ctggtggcct gtagtgcctg gctgagcgtc aacggcggcg agggtgacac
     9301 gctggcgaaa atgcttttcg gttatggact gctgcaactg ctgtttatgc tacgtctgat
     9361 gccatggtat ctctcccagc catttaatgc ttcattctgg agtttctcgt tcggcgtatc
     9421 tgcactggca accaccggtt tgcatctggg gagtggcagc gataatggat ttttccatac
     9481 gctggcggtg ccgctgttta tctttaccaa ttttattatt gcaatactgc tcatccgtac
     9541 ttttgcgctt ctgatgcagg gaaaattgtt agtcagaacc gagcgcgccg ttttaatgaa
     9601 agcagaggac aaagaatgat cattcgtgac gaaaactatt ttactgataa atatgaatta
     9661 acccgcacac actctgaagt actggaagcg gtgaaagtgg ttaaaccggg taaaacgctg
     9721 gatctgggct gtggcaatgg tcgtaacagt ctttacctgg cagccaatgg ttatgatgtt
     9781 gacgcatggg ataaaaatgc catgagtatc gccaacgtcg agcgcattaa atccattgaa
     9841 aatctggata atttacacac ccgggtcgtt gatctgaata acctcacatt tgatggacag
     9901 tacgatttta ttctttcgac tgtggtgctg atgttccttg aggctaaaac catcccaggg
     9961 ttgattgcca atatgcaacg ttgcactaaa cctggtggtt acaacctgat tgtggcggcg
    10021 atggataccg ctgattatcc atgtaccgtc ggcttcacgt ttgccttcaa agagggagaa
    10081 ttacgtcgat attacgaagg ctgggagagg gtgaaataca atgaagacgt cggcgagctg
    10141 caccgcaccg acgccaacgg taatcgtatt aaactgcgtt tcgccacgat gctggcacgt
    10201 aaaaaatgac ccggtaagca caaaacgcgt gaaaattccc cacgctgaga tgatttactg
    10261 ttcttctttt cggtaagcat attttttatc gaagggatgt gaaattaatc acagtagtcg
    10321 aagtttttta gcagcttaac ttactgaaat ttaactactg atgattgact tagccccttt
    10381 ttcggcattg actatgtcgt ctgaaaaggg gctgaaaaat ttattttcac caacactttt
    10441 tttgccacaa cacgaagcgg cgctttttgc tataacttag aaagtaatat aatcatctca
    10501 ggaaactatt catgcgtacc acatcatttg cgaaagttgc agctttatgc ggcttgttgg
    10561 ctctgtctgg ttgtgcctct aaaatcaccc agccagataa atataacaat tactctgatt
    10621 taaaagaaac aacctcagca acaggtaaac ctgttttacg ttgggtagac ccgagttttg
    10681 atcaaagcaa atatgacagc atcgtctgga acccaatcac ttattatccg gtaccaaaac
    10741 cttctaccca ggtagggcag aaagttctgg ataaaatttt gaactatacc aacaccgaaa
    10801 tgaaagaagc gatagcgcag cgtaaaccag tggttaccac cgctgggccg cgtagtctga
    10861 ttttccgtgg ggccattacc ggtgtagata ccagcaaaga agggctgcaa ttctatgaag
    10921 tggttcctgt tgcattagtg gttgcgggga cgcaaatggc tacaggccac cgtaccatgg
    10981 atactcgcct ctattttgaa ggtgagctga ttgatgcagc gactaataaa ccggttatca
    11041 aagtcgttcg tcagggcgaa ggtaaagacc tgaataacga aagtacgcca atggctttcg
    11101 aaaatattaa acaagttatt gatgacatgg cgaccgatgc caccatgttt gacgttaaca
    11161 aaaagtagtt ccagacgcgc catcgttaga tggcgctttt tatccggtgc gccgtaaaac
    11221 cccatccttc agggcgggga tataaggcgc ggttttccac ctgaccaggt gtttgctgtt
    11281 gttcaatgta ttggcggatg acggatattg gcgcacctcc gc
//
LOCUS       DAJTRH010000114        11299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTRH010000114 DAJTRH010000000
VERSION     DAJTRH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(194..1609)
                     /gene="phoA"
                     /locus_tag="OO339_003366"
     CDS             complement(194..1609)
                     /gene="phoA"
                     /locus_tag="OO339_003366"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5927876.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1710..1970)
                     /gene="iraP"
                     /locus_tag="OO339_003367"
     CDS             complement(1710..1970)
                     /gene="iraP"
                     /locus_tag="OO339_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5927877.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2152..2355)
                     /locus_tag="OO339_003368"
     CDS             complement(2152..2355)
                     /locus_tag="OO339_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927878.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2433..3527
                     /gene="ddlA"
                     /locus_tag="OO339_003369"
     CDS             2433..3527
                     /gene="ddlA"
                     /locus_tag="OO339_003369"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5927879.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3551..3763)
                     /locus_tag="OO339_003370"
     CDS             complement(3551..3763)
                     /locus_tag="OO339_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5927880.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4023..4331
                     /locus_tag="OO339_003371"
     CDS             4023..4331
                     /locus_tag="OO339_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5927881.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4390..5484)
                     /gene="yaiW"
                     /locus_tag="OO339_003372"
     CDS             complement(4390..5484)
                     /gene="yaiW"
                     /locus_tag="OO339_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5927882.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5497..6717)
                     /gene="sbmA"
                     /locus_tag="OO339_003373"
     CDS             complement(5497..6717)
                     /gene="sbmA"
                     /locus_tag="OO339_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5927883.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7069..8226
                     /gene="ampH"
                     /locus_tag="OO339_003374"
     CDS             7069..8226
                     /gene="ampH"
                     /locus_tag="OO339_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5927884.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8227..8730)
                     /locus_tag="OO339_003375"
     CDS             complement(8227..8730)
                     /locus_tag="OO339_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5927885.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(8818..9345)
                     /locus_tag="OO339_003376"
                     /pseudo
     CDS             complement(8818..9345)
                     /locus_tag="OO339_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9365..10512)
                     /locus_tag="OO339_003377"
     CDS             complement(join(9365..10275,10275..10512))
                     /locus_tag="OO339_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5927886.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
     gene            10570..11299
                     /locus_tag="OO339_003378"
                     /pseudo
     CDS             10570..11299
                     /locus_tag="OO339_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT         2798 a         2902 c         2687 g         2912 t
ORIGIN      
        1 ggttctctcg gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag
       61 taatgttatt ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt
      121 gatctgccat taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact
      181 gccgggcgca gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct
      241 ggtcggtcag tccaacaaca ttggcggcat gcgggccata cgccgcaata cgcaactgac
      301 tgccggtatg ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc
      361 catctttagt atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct
      421 ggctggcgtg ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga
      481 attccagcgc ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag
      541 ggttcgcagc gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt
      601 tctcattttt actcaacaat tcaatggctt tgtcggtcat ctgtgccagg gttggtacac
      661 tgtcattacg ttgcggatta ggcgtatagg tgactgcggg cttatcgata ttgccgtggt
      721 acgttgcttt cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca
      781 ggggtttttg ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact
      841 gataaccacg cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg
      901 ccgtttcagc aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca
      961 gctgttcggt aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac
     1021 tggtcgcgct cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg
     1081 tggcatcctg caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca
     1141 tttccagaat cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg
     1201 ttttgacatc ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt
     1261 tgccagtttt tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga
     1321 tacctttaaa aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat
     1381 cccccatccc atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat
     1441 cacgcagagc ggcggtctga tcacccgtta agcggcgagc accgccgggt gcagtaatat
     1501 cgccctgagc agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag
     1561 gggtaaacag taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc
     1621 catgtacaaa tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact
     1681 ttatgacagc tgttgaaaag attaactttt tactgacaag gatgcttcaa taacttcttt
     1741 acgtaatcgc gcagcagctc tgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg
     1801 tacagcgccc cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga
     1861 agcattgcag tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct
     1921 tcttcttttt gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt
     1981 ccttatccaa agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt
     2041 attattttta tgacaaagga aatactttgc gcaaatattt tttgtttgat tgccagcgaa
     2101 acgtttctct tatttaaata attacccaca caaaatatag gcttttaatt atcaaccttt
     2161 attataaata aaagctatga aatatattct cttcgttaac ttataattaa gagaaaaaaa
     2221 ctcaccgcat tgccatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat
     2281 cttgcgcaca tctttgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc
     2341 agaagattgc aacaatcttt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat
     2401 gcactttgtg ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt
     2461 ttggtggtaa atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg
     2521 ccattgataa aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc
     2581 acgtcagcga tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc
     2641 gcccttcggc gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag cttatcgacg
     2701 cgcaaaacgg tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc
     2761 tgggcgaaga tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt
     2821 ctgatgttct ggcttcagca gcctgtatgg ataaagatgt caccaaacgt ctgctacgcg
     2881 atgccgggct gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca
     2941 gttttgccga agtggagtct aaactggggt taccactgtt tgtaaaaccg gctaatcagg
     3001 gctcttctgt tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc
     3061 tggcgttcga gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg
     3121 aatgcgcagt tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca
     3181 ccagcgattt ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag
     3241 ttccggcagc cattgcacca gagatcaacg ataagatccg ggcgattgcc gttcaggctt
     3301 atcagacgtt gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg
     3361 aagtggtgat caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga
     3421 agctgtggca agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg
     3481 cgctggagcg tcacgctgcg gataacgcat tgaaaaccac aatgtaatat tttaacgccc
     3541 gataatattc ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg
     3601 ccagccagaa actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg
     3661 cttcaaatcc cagtaccccc accacgccat taagaatgaa tataaggcca atggcgaacg
     3721 catagtagtg ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga
     3781 aaaacaacat ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac attttcgtaa
     3841 ttattaataa atttaactta aggttgccat tttgtgactc acaacgaaga ttcacgcttc
     3901 gggaatattc ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc
     3961 agacttgcca taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt
     4021 ttatggctga tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt
     4081 caacgcctgg caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc
     4141 aattgctgaa cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg
     4201 aaacaggtcg cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc
     4261 cgtttttagc aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc
     4321 gccgccagta aaatttacgt tgccagatgc ggcctgaaac gccttatctg gcctgacatt
     4381 cctgtttgat cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca
     4441 ggccgtagtc aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc
     4501 gcggggtagt gatttacccg ttttcgcttc ggcaagttga taaactttct tgtacagcgc
     4561 cgtctcctca aaagagaagc tgtcaccttt ctctaactga cggcgaattt cgctgtcgct
     4621 catccccagt tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact
     4681 gtcgtaacga attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgactgc
     4741 gttctgaaac gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaagcggta
     4801 tatcggtgcg ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc
     4861 gccgcggcga ctgaagactt cctgacgaac tgtaccgtcc attttccacg gatacccttt
     4921 ggtatgctgt tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt
     4981 aagcgaacca aacagcgtct gccccattgg taccatgctg atcagatcgt caaaaatcgc
     5041 gctcaattgc ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg
     5101 cgatttgatt tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc gttcagcacg
     5161 acggtcaatc tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata
     5221 atttgactcc tgctgcgcca ccgccagcac cgagtagacg ttttccagcg tcggtgccag
     5281 cccctgactt tcaaaggtgg tggcgagatc tttcgcccag gcatcgcggt ctttcacgct
     5341 cgcaggcatc ttctgccgca cgacgctcgc tacatctatc gctttttcgc cctttttcaa
     5401 cggttgcgga gcctgactac tacaccccgc taaaaccaga accgccaaca atgacagcga
     5461 agatagatga ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc
     5521 ctgaattttg tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat
     5581 caactcaacc agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc
     5641 aaaaacgttg gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg
     5701 aaacagcaag aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt
     5761 gaaatacata tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc taaacagctc
     5821 gcgtaccgta ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg
     5881 gtaggcggcc tctactcgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac
     5941 tgccagcaat ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg
     6001 aatgtgcccg ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag
     6061 gaaggcgatc aacgtcatga tggcgttgat aaaactgacg cccatattct ccagcgttga
     6121 agcaaaacgc atggtgtctt cctgcacacg ctgtgcggca ccttcgatat gacgcagttg
     6181 ttgccagttc gccatgtaat attcgttcat cgccgtacgc cagcggaaca cgtagtgact
     6241 gacaaagaag ttgttcagta cactgatcac caccgcgatc agcgcaatcc ccagaaagac
     6301 gcccacttcg cggtaaaatt gttcgatagt gactttatgc ggcgaactta gcgcggtttg
     6361 aatcagatca tagaacggcg cataccaggc gttgacggcg accccgactt ccaccaaaaa
     6421 ccaagtgacg aagataatca gcgcagtacc gagtattgac cagtattgcc aacggtgcgg
     6481 gctgtagata aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa
     6541 aatcaggaaa tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc
     6601 ggtgatacgt gccacccagt cacccccacc ggcttgccag aagataacgg caatcaatgc
     6661 ccaaacaaag gccgagagaa aaaacgttcc cggctttggg aaaaaagact taaacatatg
     6721 cactcctgct aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccgctac
     6781 aaagcgtcag ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag
     6841 cctgaccata agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga
     6901 ttaacacgcg caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg
     6961 cgccgttacg tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag
     7021 tagtataaat acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt
     7081 ctgctttttt ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg
     7141 atcactgaac cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac
     7201 ggcagcggtg ccacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgt
     7261 agttatggtg aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt
     7321 attgcttccc tcaccaaact aatgaccagt gaaatgctgg tgaaattgct cgaccagggg
     7381 accgtaaagt taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccgacc
     7441 tacaatggga cgcccattac cctagtgaat ctggcaaccc ataccagcgc cctgccccgt
     7501 gaacagcccg gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc
     7561 tggaaatacc tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct
     7621 aaccttgcgt tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc
     7681 cagttgtttg aagagcagat tacccatcca ttggggatga aagacaccac ctacaccccc
     7741 tcaccggatc agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac
     7801 acgctggcgg caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg
     7861 atgcagcagt atttgtcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag
     7921 acgttgattt atcagcgcgc gcagtttacg aaagtgattg gcatggatgt ccccggcaaa
     7981 gcggatgcgc ttggtttagg ctgggtatat atggccccga aagagggtcg tccgaggatt
     8041 attcagaaga caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag
     8101 aatattggcg cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc
     8161 gatggcatca atgatctggt gaccgagcta agcgggaata aaccgttggt tatccccgca
     8221 tcctgattaa tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc
     8281 gatatagccg cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg
     8341 tgtacgttga tggatataat tcatcacacc aatacgtgat cgcaattctt cattccagtc
     8401 aatcattgat aataatgttg cgcgggtttg tttggctggt agatgatatc ccgtacaatt
     8461 tccttctgat attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat
     8521 aatataagga gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac
     8581 tccttctaaa ataacgaaag tatcttcgtt attgacatca ctggaaaata taacttgctt
     8641 ttcattatta aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa
     8701 ttcctgaaga ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa
     8761 atccttgacg ggcattattt agattaaatt accagtattt cttcggagtg aagaatatta
     8821 ccaagtatat ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag
     8881 gtagttagca tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc
     8941 aacacgtacc gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc
     9001 gttgttagag tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc
     9061 gctgtaggtg aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaagg
     9121 ctgaccgtca actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc
     9181 agaaatgctg ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt cgtaaccggt
     9241 tttcaaaccg aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag taccgttgct
     9301 catggttgca gacaggtcgt tgttgaagtg agagtagctc aagctatgaa tcgccacgga
     9361 taatctagac acttccgagc cgttgataat actggttttc atattctgtc ggtgacatct
     9421 gttcgctaga accatgccga cgcttactgt tataaaacat ttcgatgtaa tcaaaaatat
     9481 cactgcgggc ttcttcccgc gttccgtaga tctttttctt tatccgttca cgtttcaaca
     9541 actggaaaaa actttctgca accgcattat catggcagtt accgcgacgg ctcatgctac
     9601 cctccaggcc gtgtgatttc aggaacgact gccactcatg gcttgtgtac tgactgccct
     9661 gatccgaatg aaccagcacc tgtttttcgg gattacgccg ccatacagcc atcagcagtg
     9721 cgttcaggac aatgtccttt gtcatccggg attgcatgga ccagccgata attttgcgtg
     9781 agaacagatc aacaacaacg gcaagataca gccagccttc gtgggtcctg atgtaggtta
     9841 tgtccgttac ccaacgctca tcaggagcat ccggattgaa ctgtcgctgg agcctgttgg
     9901 gtgacacgat actggcctcg cctttacgtg cccgcgggct tcggtatccg acctgagcct
     9961 ttattccgac acgtttcatc agtctccaga ctctgtttac tccgcactgt tgcccgctgt
    10021 cacgcagatc cagatggatt ttgcgataac catagacgca tcccgattcc agccagaact
    10081 gtttaatctg tcctgtcagt ctcaggtctg cctgatggcg ttgtgaatgc ggctgctgaa
    10141 gccaggcgta aaaaccactg ggatgaacat ccagcacccg acagagcagg cgaacaggcc
    10201 agcaacagga gttgtcacgg ataaaggcgt acctcagtcg gacagctttg cgaagtacgc
    10261 cgcggctttt tttaatatgt cccgttcgtc ggtaacccgt ttcagctctt tctggagacg
    10321 gcggatctcg gcctgagcat ctgactgttc tttattagtg gaagaatccg gaccgtactt
    10381 ctttatccag gcataaaggc tgtgggtggt gatatcgaga cgtgttgcaa cgctggcaac
    10441 agaataaccg cgatcaacaa cctgtttgac tgcttcagtt ttaaactctt cgggataacg
    10501 cttaccgctc atgggcacct ctctttaagc catcttaaat gactctgagg tgtctgttaa
    10561 acccgtggcg attcacaatt ctcttgcacc attaactatg ttcagtccct gcgcgagttg
    10621 tacaccgatg tcatcgaaga gttttactgg gttgcgctcc cgcttacgac gcaaaattcc
    10681 ctttcacaat accaacccga atggcagtgc tgggaacctg atgtcgaatg ggtgcgtcag
    10741 cccccgcaag atgcgataac cgctcctgac tttttctgct tttaccagcc aggcatgacc
    10801 ttcgaacaat ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc ggcggcgatg
    10861 atgatcggca tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc cagtttaaat
    10921 aaacaacgtt ttgccgacga taaaccatgg actacggccg caccaggcgg tcatagctgg
    10981 tacatttacc ccatttacga ctggaaagtg gctgatatct ggacctgata tgcaaatcat
    11041 caaaatctct gcaacccact gtataaccta atgtatcagg caggcgttcc tctgcgtcat
    11101 atgcgaattt gcgaaccttt tggcccggag caacgacaag gattatggct ctgtcacgtt
    11161 atcgaaccgg atcgctgggc tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc
    11221 atttacgccg gacatgacaa tcatttctat gggcatcgga aaatcctcaa accagaacat
    11281 ttagactggc aagaatatg
//
LOCUS       DAJTRH010000115        11275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTRH010000115 DAJTRH010000000
VERSION     DAJTRH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..612)
                     /gene="hdhA"
                     /locus_tag="OO339_003379"
     CDS             complement(<1..612)
                     /gene="hdhA"
                     /locus_tag="OO339_003379"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5927887.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITPE"
     gene            complement(724..1752)
                     /gene="malI"
                     /locus_tag="OO339_003380"
     CDS             complement(724..1752)
                     /gene="malI"
                     /locus_tag="OO339_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5927888.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1927..3519
                     /gene="malX"
                     /locus_tag="OO339_003381"
     CDS             1927..3519
                     /gene="malX"
                     /locus_tag="OO339_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5927889.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3529..4701
                     /gene="malY"
                     /locus_tag="OO339_003382"
     CDS             3529..4701
                     /gene="malY"
                     /locus_tag="OO339_003382"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5927890.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4805..5806
                     /gene="add"
                     /locus_tag="OO339_003383"
     CDS             4805..5806
                     /gene="add"
                     /locus_tag="OO339_003383"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5927891.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5842..6882)
                     /locus_tag="OO339_003384"
     CDS             complement(5842..6882)
                     /locus_tag="OO339_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5927892.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7125..7250
                     /gene="blr"
                     /locus_tag="OO339_003385"
     CDS             7125..7250
                     /gene="blr"
                     /locus_tag="OO339_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5927893.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7523..7738
                     /gene="ydgT"
                     /locus_tag="OO339_003386"
     CDS             7523..7738
                     /gene="ydgT"
                     /locus_tag="OO339_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5927894.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7824..8264
                     /locus_tag="OO339_003387"
     CDS             7824..8264
                     /locus_tag="OO339_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5927895.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8341..8922
                     /gene="rsxA"
                     /locus_tag="OO339_003388"
     CDS             8341..8922
                     /gene="rsxA"
                     /locus_tag="OO339_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5927896.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8922..9500
                     /gene="rsxB"
                     /locus_tag="OO339_003389"
     CDS             8922..9500
                     /gene="rsxB"
                     /locus_tag="OO339_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5927897.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9493..>11275
                     /gene="rsxC"
                     /locus_tag="OO339_003390"
     CDS             9493..>11275
                     /gene="rsxC"
                     /locus_tag="OO339_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5927898.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAA"
BASE COUNT         2626 a         2905 c         2842 g         2902 t
ORIGIN      
        1 ttctggtgta ataacggatt tcagggcatc ggttaatatt gcccccggcg caatgctatt
       61 cacccgaata tttttttcac ccaggtcaaa tgccatattt ctgaccagat gactggccgc
      121 agctttagat gatgcatagg aagtcatgtt tatattttta ttttctgccg ccatagaagt
      181 gatggtcaga ataacgccac ctccattttt ttccatttct ggcgcaacaa gttgtgacag
      241 atggaaaaaa gaaaacacat tcagttcata agcacggcga aaatctgcca ttggcatatc
      301 aaacggttta ggaccaccgc caccggcgtt gttaaccagg atatcaactt tacccagatt
      361 actgacggca aagtctgcca gtgcagagag ttcctgttcg gaagtaatat cacaacgcca
      421 ggcaaatgcc tgaccatcca gttgttgaat ttcgtctaca acatggttag ctgcgtcggc
      481 gttaatatca ctgaccacca cagatgcgcc agctgtcgcg aatgtaatgg cgatttcttt
      541 accaatacct gcacccgcac ctgtgatgat ggcgcatttt ccgtcgagtc tcaggttgtc
      601 agaattaaac acataaacct cctgtgaact tcagtaaaaa taaaaggcac cgcctgtgtg
      661 ccacattttt tatcattaac cttgaggaaa ggacactacc aagtatagca gataaagaga
      721 ggattatttc gccgcaatga gccgggcggg aataataaga ttgcgtgaat gcgtctcttc
      781 atgggtgatt ttttgcatca tgcgatccgc aagtgtgata ccaagttccc gcgctggcgt
      841 gctggcccag gtaacaggta tatcatcaag tgtggttggt gtcgcatcgg taaatgccgc
      901 cagcgaaacc tgttgctcaa agtaacgatc gactccgctt tccccgcttt gccgccctgc
      961 tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg ttatagcaaa ccacggcact
     1021 gatggtcggg ttatgacgta aaagcgccgt gatagcttcc gcggcttgct tctggctgga
     1081 agtgcactcc aacacccaat cgctgtgaaa cggcaggcca aattttagta gagttgcaca
     1141 atagcccccc acccgttctg cacgggttaa tgaggaactt tgccctccca gccaggcgat
     1201 ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac tgtgcagcct gcatgttgtc
     1261 cgggcgaacc gtatcaacat catcaagata actggcacgg gaagcgaaaa tcaccgggat
     1321 agctttttct tctgccatcc gccgcaggtc atcgctgctt cctgcagccc cggcaattac
     1381 cacaccatcg acaccctgat tcagtaacag tgaaaaccgc tgtgccagct gctcgccgtc
     1441 tttaccgccg tgaagcaaaa aaaccatccg tccctgtgct tccagagctt ccgtcaatcc
     1501 ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg acgatcaaac caatgacgcc
     1561 gctttgcccg ccgcgcagcg ccgacgcctg gcgattgcgc acaaatccca gctcttcaat
     1621 ggcggcgtta acgcgttctc ctgtggcggt agagattcgc cctttgccat taagcaccag
     1681 cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca tcatgaatgg ttattttttt
     1741 ggcggtagcc atgcagatga cctactccct gattatgtga cagataaaac gttttacctt
     1801 ttatttatct tatacccgct attatcgttg cgtaatgtga tttatgactc actaaaattt
     1861 gataaaacgt tttatcttct cgcgcaattt actgaatcca gattgttctc tacgaggagt
     1921 cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt gggagttctt ccagcagtta
     1981 ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct gcggcattat gctcggcatt
     2041 ggtagttctc ttagcagcca tgatgtcata accctgatcc cggtcctggg caaccccgtg
     2101 ttgcaggcta tctttacctg gatgagtaag attggctcgt ttgcttttag tttcctgcct
     2161 gtcatgttct gtatcgccat cccgctgggc ctggcacgcg aaaataaagg cgtagcggca
     2221 ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg taaacttctg gttgaccaat
     2281 aaaggcattc tgccaaccac agatgccgcc gttctgaaag ccaataacat ccagagcatt
     2341 cttgggatcc agtcgatcga taccgggatc ctcggtgcgg tgatcgccgg tattatcgtc
     2401 tggatgctgc atgagcgttt ccataatatc cgcctgccgg atgcgctggc attcttcggc
     2461 ggtacgcgct tcgtaccaat tatctcctcg ctggtgatgg gccttgtcgg cctggtgatt
     2521 ccattagtct ggccgatttt cgccatgggt attagcggct tgggccatat gataaacagc
     2581 gcgggtgatt tcggaccgat gctgtttggt accggtgaac gtctgctgtt gccgtttggt
     2641 ctgcatcaca ttctggtggc attaattcgc tttaccgacg caggcggcac gcaggaggtc
     2701 tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg cgcaattgag ttgcccgacc
     2761 actcacggtt tttctgaaag cgtcacgcgt ttcctttcgc aaggtaaaat gcctgcgttt
     2821 ctcggcggtc taccaggtgc agcgttagct atgtatcact gcgcgcgccc ggaaaatcgc
     2881 cataaaatta aaggtctgct gatttctggc ctgatcgcct gcgtcgttgg cggcactacc
     2941 gaaccgctgg aattcctgtt cctgttcgta gcgccagttc tgtatgtcat ccacgcgctg
     3001 ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg tcaccatcgg taataccgac
     3061 ggcaatatca tcgacttcgt ggtgttcggt attttgcatg gtctgtcaac caagtggtac
     3121 atggtgccag tggtggcggc aatctggttt gtcgtttact acgtcatctt ccgtttcgct
     3181 atcacccgct tcaatctgaa aaccccgggg cgcgatagcg aagttgccag ctcaatcgaa
     3241 aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg ttcctgcaat cctcgaagca
     3301 ttaggcggtg ccgacaatat tgtcagcctc gataactgca ttacccgtct gcgtttgtct
     3361 gtggaagata tgtcgcttgt taatgtgcag gcactgaagg acaatcgggc aattggcgta
     3421 gtacaactta atcaacataa cctgcaggtt gttatcgggc cacaagttca gtcagtaaaa
     3481 gatgaaatgg ccggtctgat gcatactgtc caggcataag gataagatat gttcgatttt
     3541 tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt gggattatgt cgctgaccgt
     3601 ttcggcactg ctgacctgtt accgttcacg atttcagaca tggattttgc cactgccccc
     3661 tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg tatttggcta cagccgctgg
     3721 aaaaacgatg agtttctcgc ggctattgcc cactggtttt ccacccagca ttacaccgca
     3781 attgatacgc agacggtggt gtatggccct tctgtcatct atatggtttc agaactgatt
     3841 cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca cgcccgccta tgacgcattt
     3901 tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg ttgctttaga gaagcaggct
     3961 gacggctggt tttgcgatat gggcaagctg gaagccgttt tggcgaaacc agaatgtaaa
     4021 attatgctcc tgtgtaaccc acagaatcct accgggaaag tgtggacgtg cgatgagctg
     4081 gagatcatgg ctgacctgtg cgagcgtcat gatgtgcggg ttatttccga tgaaatccat
     4141 atggatatgg tctggggcga gcagccgcat attccctgga gtaatgtggc gcggggagac
     4201 tgggcgttgc tgacatcggg atcgaaaagt ttcaatattc ccgcgctgac cggtgcttac
     4261 gggattatag aaaatagcag cagccgtgat gcctatttat cggcactgaa aggacgtgat
     4321 gggctttctt ccccttcggt actggcgtta actgcccata tcgccgccta tcagcaaggt
     4381 gcaccgtggc tggatgcctt acgcgtctat ctgaaagata acctgacgta tatctcagat
     4441 aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc cgcaatccac ttatctggcc
     4501 tggcttgatc tacgtccgtt gaatattgac gacaacgcgt tgcaaaaagc gcttatcgag
     4561 caagaaaaag tcgcgatcat gccggggtat acctatggtg aagaaggtcg tggttttgtc
     4621 cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag gtgtggctgg attaataaac
     4681 gccatccgcg ctgttcgcta accccaattg cgcaacgtaa aaaatcgttg cgcaatcgtg
     4741 gatttttacc ctgctttgtt tttataatgg tgcgcacttt tatatccaga aaaagagtgc
     4801 gaccatgatt gataccaccc tgccattaac tgatatccat cgccaccttg atggcaacat
     4861 tcgtccccag accattcttg aacttggccg ccagtataat atctcgcttc ctgcacaatc
     4921 ccttgaaaca ctgattcccc acgttcaggt cattgccaac gaacccgatc tggtgagctt
     4981 tctgaccaaa cttgactggg gcgttaaagt tctcgcctct cttgatgcct gccgccgcgt
     5041 ggcatttgaa aacattgaag atgcagcccg taacggcctg cgctatgtcg agctgcgttt
     5101 ttcaccaggc tacatggcaa tggcacataa gctgcctgta gcgggtgttg tcgaagcggt
     5161 gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg caggcgaagc ttatcggcat
     5221 tatgagccga accttcggcg aagccgcctg tcagcaagag ctggaggcct ttttagccca
     5281 ccgtgaccag attaccgcac ttgatttagc tggtgatgaa cttggattcc cgggaagtct
     5341 gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg catattaccg tccatgcagg
     5401 cgaagctgcc gggccggaaa gcatctggca ggcgattcgt gaactgggtg cggagcgtat
     5461 tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg gattttctcg ccgagcaaca
     5521 aattggtatt gaatcctgtc tgacctccaa tattcagacc agcaccgtag cagagctggc
     5581 tgcacatccg ctgaaaacgt tccttgagca tggcattcgt gccagcatta acactgacga
     5641 tcccggcgta cagggagtgg atatcattca cgaatatacc gttgccgcgc cagctgctgg
     5701 gttatcccgt gagcaaatcc gccaggcaca gattaatggt ctggaaatgg ctttcctcag
     5761 cgcagaggaa aaacgcgcac tgcgagaaaa agtcgccgcg aagtaacaaa aatggatggt
     5821 gcaaattgca ccatccattt ttcacgcaag gcacaacgtc gcgcgatgtt tggcggattc
     5881 gatgcccagc tcaatcaact ccattacctg gattgcctgg cttgccggaa ccggattttc
     5941 accatcgcca tttaacgcat cacgaatcgc cgcatagtaa gccggataat tcccaggcac
     6001 cgtcaacagc gtttcttcga cacgttcctc accttccacg cgggtaagta cgccatcacg
     6061 catatcgtag ccccagtctt cctgcggcag acgctcgcca tttttcagac gttcttcctg
     6121 tggatcgagg ccatatttca cataactgcc tcgggatcca tgcacgatat accgtgctga
     6181 ctcagcggct gccagcatgg taccgtgtaa aatgactcgc cgctgtggat aggacaagat
     6241 ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac tgtgccaaat ctaccgtcat
     6301 gctgaccggt aaaccaaata gcgtaatggc ctgatcaaga agatgcggtg ctaaatcgta
     6361 ccagataccg ctgcctgggc cgccctgttc acgccaacga tcgcgcacct gcggacggaa
     6421 gcggtcaaaa tgagactcaa agtaagcaac ttcacccagc acgccttccg cgagcaaacc
     6481 ttttagcgtc aagaaatcgc tatcccaacg gcggttatgg aatacagaca gcacacgccc
     6541 caggcttttt gccagcgcat ccagctctcg cgcttgtgac agtgtcacgg taaagggttt
     6601 atcaacgacc acatgtttac ccgcctcaag cgccgctttg gctaacggga aatgggtatc
     6661 gttgggtgta ggaatgacaa tcaggtctat gttgggatcg ttaaacagat gcttcggctc
     6721 agagacaacc gtaaccgttg gccagtcggc ttttactttt gtttcatcac tgctggagat
     6781 taccgccagt tccagcccgg gcgtgcccgc aatcaggggc gcatggaagg ttttgctcgc
     6841 ataaccatac ccaatcaacc caacacggat gttgtcgctc atgtcattgc ctctcattga
     6901 agtatgatgg ctatttgaca ctatccttta cccacgctca acagtttaat aacctgccag
     6961 caataaggga tgttgtttaa cttaagtcaa aaaaatagcg aattttccaa cgacaaaagc
     7021 taaatatcgc aaaaacctca gtaaaaatct tgctggagct attattgcta agtaacattt
     7081 accccctgaa gttaatggat caatcaagag agatgtgggc tgtaatgaat cgtcttattg
     7141 aattaacagg ttggatcgtt cttgtcgttt cagtcattct tcttggcgtg gcgagtcaca
     7201 ttgacaacta tcagccacct gaacagagtg cttcggtaca acacaagtaa gctctgcact
     7261 tgtggagcga catgctgccc gtccgggtgc atgttttcac ttgtcggata ttaaaccagg
     7321 aatttattat cttgttcgat gttgttggtg attgtcaggg atagtaaagc gcgttactct
     7381 tttcagagca gtttttcatc cctgaatatt tgccatcagc gcattcttat tattgtaata
     7441 acacaagcgt tcacttttgg tgaaatttta tcatcactaa taatacctac ccctttggta
     7501 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     7561 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     7621 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7681 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7741 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7801 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7861 tgcttggccc gttggcctgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7921 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7981 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     8041 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     8101 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagttg
     8161 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     8221 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     8281 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     8341 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     8401 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     8461 atggggctgg caacaacgtt tgtgatgacg ctgacgtcta tttgcgcctg gcttatcgat
     8521 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     8581 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     8641 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8701 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8761 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8821 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8881 tctctggcct ttatgggctt tagtggtttg gtgaagttgt aatgaatgct atctggattg
     8941 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
     9001 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     9061 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     9121 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     9181 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     9241 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     9301 gtccggtaga cgccatcgtt ggcgctaccc gtgtcatgca tacggtaatg agtgatctct
     9361 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     9421 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     9481 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     9541 aacggcggca tccatccacc ggagatgaaa acccagacca acggtacccc cctgcgccag
     9601 gtactactgg cgcagcattt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9661 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9721 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9781 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9841 tgctggatcc cgcgcgacgg ctgggtcgat tatcgctctc gcagtcgcga agagttaatc
     9901 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg ccggattccc gacaggcgtt
     9961 aaattgcagg gtggtggaga taagattgaa acgttgatta tcaacgctgc tgagtgcgag
    10021 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
    10081 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
    10141 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
    10201 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
    10261 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
    10321 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
    10381 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
    10441 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
    10501 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
    10561 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
    10621 tgcatccggt gtagcgcctg tgctgacgcc tgccctgtgg atcttttgcc gcaacagttg
    10681 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgccgactgc
    10741 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccttggt gcaatatttc
    10801 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10861 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcgac tcgccttgaa
    10921 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10981 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    11041 gaacgcccgg ataacagtgc aattattgca gaacgggaag cccgtaaagc gcaagccaga
    11101 gcgaaacagg cagaactgga gcaaactaac gacgcagcaa ccgttgctga accacgtaaa
    11161 actgccgttg aagcagctat cgcccgctcc aaagcgcgca agctggaaca gcaacaggct
    11221 aatgcggaac cagaagaaca ggtcgatccg cgcaaagccg ccgtcgaagc cgcta
//
LOCUS       DAJTRH010000116        11110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTRH010000116 DAJTRH010000000
VERSION     DAJTRH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO339_003391"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO339_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5927899.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO339_003392"
     tRNA            complement(621..695)
                     /locus_tag="OO339_003392"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO339_003393"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO339_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO339_003394"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO339_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5927900.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO339_003395"
     CDS             complement(2932..3990)
                     /locus_tag="OO339_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927901.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO339_003396"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO339_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5927902.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO339_003397"
     CDS             6068..6352
                     /locus_tag="OO339_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5927903.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO339_003398"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO339_003398"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5927904.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO339_003399"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO339_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5927905.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO339_003400"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO339_003400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5927906.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2778 a         2542 c         2747 g         3043 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt
//
LOCUS       DAJTRH010000117        11014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTRH010000117 DAJTRH010000000
VERSION     DAJTRH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..760)
                     /locus_tag="OO339_003401"
     CDS             complement(<1..760)
                     /locus_tag="OO339_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5927907.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANN"
     gene            1251..1382
                     /locus_tag="OO339_003402"
     CDS             1251..1382
                     /locus_tag="OO339_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5927908.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            1642..2637
                     /gene="pgl"
                     /locus_tag="OO339_003403"
     CDS             1642..2637
                     /gene="pgl"
                     /locus_tag="OO339_003403"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5927909.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2678..3631)
                     /locus_tag="OO339_003404"
                     /pseudo
     CDS             complement(2678..3631)
                     /locus_tag="OO339_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3815..4867
                     /locus_tag="OO339_003405"
     CDS             3815..4867
                     /locus_tag="OO339_003405"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5927910.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4942..6375
                     /locus_tag="OO339_003406"
     CDS             4942..6375
                     /locus_tag="OO339_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5927911.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6558..8738
                     /locus_tag="OO339_003407"
     CDS             6558..8738
                     /locus_tag="OO339_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5927912.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDRHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8879..10162)
                     /locus_tag="OO339_003408"
     CDS             complement(8879..10162)
                     /locus_tag="OO339_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5927913.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(10297..10989)
                     /locus_tag="OO339_003409"
     CDS             complement(10297..10989)
                     /locus_tag="OO339_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5927914.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAATRA
                     AKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         2746 a         2481 c         2734 g         3053 t
ORIGIN      
        1 ggttatttgc aatctcaata gttatatgag gatctaaacg aaaaagattt gtcgggattg
       61 tagttatttg gttgttttca aaataaaaaa atcttctcat gataatattt tcttggatgt
      121 ttggtaagtt gcttaatcta ttgctatatg caaaaagaag ttgcagtctc ctaggtaaag
      181 ggggcagttc agtaagatgg ttgtgcgcaa ccttaagtat cttaagatta gaaggcagag
      241 atggaagagt ttgcagttcg ttgttcctaa cctcaagttt ttttaaattc aagggtaatg
      301 aaggcagtgt ggataaccta ttatactctg cgcttagtga tttgatggtg tctggtaata
      361 acggcagatt ctctaattga ttttcggaca catccaaagt tattagataa tgtggtaatg
      421 cagatatatg tgttaagaaa ttatctcttg caataagttc tttaatattt tcaggtaaat
      481 cagtaaggat ggttaattta ttacagctca catcaagctt tttaatgcaa gcagggagta
      541 caggtagcga ggaaagcgcc aaggctcgca ggtccagttt atgcccctta gaggtaagac
      601 atgcacgaag tcttcttacc gcctcagtac gctgttcttc cggtaacccc tgtttctccc
      661 aatcatccca ttcggacaaa taatggttgg gggatgtaat tgtgctgata tgtggaaata
      721 aacaggatga aatgtttctg agcatattaa tttctcgcat atggatatgg aattagattg
      781 tgaataaatt gtatttaatg tatctctcct aatgaggagg gtgtttcaga atattcgcca
      841 taagggaatg agtgttatat catgctttct accctagtaa gtatatttgc ttttttcgta
      901 tatttggttc atcttttttt gatgttttac atattcacaa tgcagaggat ttataccaga
      961 agacattagt cttgagtaag atactacaac atctccaact attttagcca gcctgactac
     1021 ttgacttggt aatatttgtt gctaataatc atgtataaaa taagcttata tacatagtaa
     1081 cttcagcaaa ggatttagta atgatcaaaa tcatatctca atgtactgtt tttttcttta
     1141 aaaaaacagt acattctatt ttataatgac tgtgtattcc catggaagga gatcacccaa
     1201 ttctcccccc cgacttttga cgcagtgtct tgttaacagg gggattgacc atgcttattg
     1261 gctatgtacg cgtatcaaca aatgaccaga ataccgatct acaacgtaat gcgtttattt
     1321 gtgccggaag tgagttgatt gttgcgcgaa tgggaagttg gccagaagtt gttatggcat
     1381 agtgtttacg gtcgttgata caggtaaaca aaagcgggga gagtcatact ccctgaatag
     1441 tcgcgaaaat atctgtggca ttgtcaaatt cgtgtcggtg actaatattt tagtaatatg
     1501 ccattcgaaa tggagtgcta acttattgat ttaattgatg tgcgatattg tattttatcg
     1561 tgttttgttg tctgtttttt atccagtaag tgttgttata tgtatgaaag ataagggatt
     1621 tatcattttt gaggtgtgtt catgaagcaa acagtttata tcgccagccc tgagagccag
     1681 caaattcacg tctggaacct gaatcatgaa ggcgcactga cgctgacaca ggttgtcgat
     1741 gtgccggggc aggtgcagcc gatggtggtc agcccggaca aacgttatct ctatgttggt
     1801 gttcgccctg agtttcgcgt cctggcgtat cgtattgccc cggacgatgg cgcactgacc
     1861 tttgccgcag agtctgtgct gccgggtagt ccgacgcata tttccaccga tcaccagggg
     1921 cagtttgtct ttgtaggttc ttacaatgcg ggtaacgtga gcgtaacgcg tctggaagat
     1981 ggcctgccag tgggcgtcgt cgatgtagtc gaggggctgg acggttgcca ttccgccaat
     2041 atctcaccgg acaaccgtac gctgtgggtt ccggcattaa agcaggatcg catttgcctg
     2101 tttacggtca gcgatgatgg tcatctggtg gcgcaggacc ctgcggaagt gactaccgtt
     2161 gaaggggccg gcccgcgtca tatggtattc catccaaacg aacaatatgc gtattgcgtc
     2221 aatgagttaa acagctcagt ggatgtctgg gaactgaaag atccgcacgg caatatcgaa
     2281 tgtgtccaga cgctggatat gatgccggaa aacttctccg acacccgttg ggcggctgat
     2341 attcatatca ccccggatgg tcgccattta tacgcctgcg accgtaccgc cagtctgatt
     2401 accgttttca gcgtttcgga agatggcagc gtgttgagta aagaaggctt ccagccaacg
     2461 gaaacccagc cgcgcggctt caatgttgat tacagcggca agtatctgat tgccgctggg
     2521 caaaaatctc accacatctc ggtatacgaa attgttggcg agcaggggct actgcatgaa
     2581 aaaggccgct atgcggtcgg gcagggacca atgtgggtgg tggttaacgc acactaaccg
     2641 ctgatttaac cggcgcagtc tctcctgcgc cggtgtatta acttatctcc tgtaacgcat
     2701 gtctctggcg ttcgacgata ttggtccaca aattgtcttt atcgtcagtc cacaaattaa
     2761 tcagcaagga aaaaaagcgt tcagctgccg gagaaagcac ggcatctttg cggcgaataa
     2821 tccccaatgt ccgacgtata acgggttcca ccagtgggat accaataatc gaagaatagg
     2881 gcgcatgggg cattgccaga ccaggaagcg ccgaaatccc cagtcctgcc tccactagtc
     2941 ctaatgacgt cgaaagatga cgcacttcgt aaaaccagtc cagcttccag ggcttgtcgg
     3001 ctagctgttg ttctatcaac aatcggttgc cgctggagga acgtacgcca atcattttgt
     3061 aacccactaa ctcctgccat tctacaagct actttttggc caatggatgg tcacgccgac
     3121 aggccagcac gaatggctcg ttgaccagtg gggtaaaatc aatggatgaa tttgtgacgt
     3181 tgttcatgtt tatgccaaag tctgattcgt tacacagcac cgattccatg caattattcg
     3241 tgccttgttc cagaatccgc actttaatat tgggatacag ctcattaaat ttaccgattg
     3301 ccagcggtaa aaaataaaat actgcggtcg gaatacacgc taatgtcacc ataccacgat
     3361 gataagcatt catatcacga atattaaaaa gcgtttcatc gaatttcttt attaattccc
     3421 gcgcctccgg gagcaacctt tttcctgctt ttgttaacgt aaccttgcga gttgtacgct
     3481 caaatagttg aatatgtaaa tcttcctcca tcttttttat tcttcgcgtt aatgcaggtt
     3541 gcgtaatatt gagtaattta gcggcattgt taaatgaact ggactctgcc agtatgacaa
     3601 atgccttcat acttgataat tcatgcttca ttattactcc ggaaaatgga agcgacgatt
     3661 ttggggggct ggtcgttaaa aattttaact gcatttagcc aacttaaatt aatgaaaaaa
     3721 tgttattaat cgttgagcta aagtcattag agatgctttg tccttaatgt aaccatatcg
     3781 caataagtta tgtttttaaa ttgagggcat tattatgaaa aaaataccct gcgtgatgat
     3841 gcgaggtgga acctcgaggg gcgcgttcct gttagcggaa catttacccg aagatcaaac
     3901 gcagcgcgat aaaatattga tggcaattat gggttccggt aacgatctgg aaattgacgg
     3961 tattggcggc ggtaatccac tgaccagtaa agtcgccatt attagccgtt ccagcgatcc
     4021 gcgtgctgat gtcgattatc tgtttgctca agttatcgtc catgagcaac gtgtcgatac
     4081 cacgcctaac tgcggcaata tgctgtctgg cgtaggggca ttcgccattg aaaatggttt
     4141 gattgcagcg acttcgccag ttacccgtgt acgtatccgc aacgtcaata cgggtacgtt
     4201 catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag tacgagggta gcgccagaat
     4261 tgacggcgta ccgggtactg ccgcaccggt tgcgctcact ttcctgaatg ccgctggaac
     4321 gaaaactgga aaagttttcc cgactgataa tcagattgat tattttgacg atgtcccggt
     4381 gacctgtatc gatatggcga tgccagtcgt cattattccg gctgaatatc tgggaaaaac
     4441 aggttatgaa ttaccggcgg aactggatgc cgacaaagca ttattagccc gcattgaatc
     4501 tatccggcta caagcgggta aagcaatggg cttaggtgat gtcagtaata tggttatccc
     4561 taaacctgtg cttatttctc cagcgcagaa aggcggggca attaatgtgc gttattttat
     4621 gccgcattct tgccatcgcg cgctggcgat aaccggtgct attgctattt ccagtagttg
     4681 tgcattggaa ggcaccgtca cccgacaaat cgtcccttct gtaggatatg gcaatatcaa
     4741 tattgaacac cccagtggtg cgctcgacgt tcatttaagt aatgaaggtc aggatgccac
     4801 gacgttacgc gcatctgtta ttcggacaac cagaaaaata ttttccggtg aagtttatct
     4861 tccctgaaaa attcgttgtc aggataagga caatcaataa aggacttctg tatgagtcat
     4921 acagaaagaa caggatttta aatgaataag aaatcgttat ggaagctaat tctgatatta
     4981 gcgatcccat gtattattgg ttttatgcca gctccggcag gattaagcga actggcgtgg
     5041 gtgctttttg gtatttacct ggcggccatt gtgggtctgg ttatcaagcc tttcccggaa
     5101 cctgtcgtac tgttaattgc cgttgctgcc tcaatggtgg tggtcggtaa cttatccgac
     5161 ggtgcgttta agaccaccgc cgtattaagc ggttactctt caggtactac ctggctggtg
     5221 ttctcggcgt ttaccttaag cgccgcattt gtgaccaccg gtttaggtaa acttattgcc
     5281 tatctgctga ttggtaaaat cggtaacacc atgctgggtc tgggttacgt tacggtattc
     5341 ctcgatctgg tactggctcc ggcaacaccg tctaacaccg cgcgtgcggg cggcattgtg
     5401 ttaccgatca tcaacagcgt ggcggtggct ttggggtccg aaccggaaaa aagtccgcgt
     5461 cgtgtcggac attacctgat gatgtccatt tacatggtca ccaaaaccac cagctatatg
     5521 ttctttaccg caatggcggg gaacattctg gcgctgaaaa tgatcaacga cattctgcac
     5581 ctgcaaatta gctggggtgg atgggcgctg gccgccggat tgccgggcat cattatgctg
     5641 ctggtcaccc cgctggtaat ttacaccatg tatccaccag aaattaaaaa ggtggataac
     5701 aaaaccatcg ctaaagcggg ccttgccgaa ctgggaccga tgaaaatccg cgaaaaaatg
     5761 ctgctcggtg tctttgtgct ggcgctactg ggctggattt tcagtaagtc tctgggggtt
     5821 gatgaatcca ctgtggcaat cgttgttatg gcgaccatgc tgctgctggg tatcgttacc
     5881 tgggaagacg tggttaaaaa taaaggcggc tggaatacct taatctggta cggcggtatt
     5941 atcggcttaa gctccttatt atcgaaagtt aaatttttcg aatggttagc tgaagtcttt
     6001 aaaaataacc tggcatttga tggtcacggt aacgttgctt tcttcgttat tattttcctc
     6061 agcatcatcg tgcgttattt cttcgcttcc ggtagtgcct atatcgttgc tatgttacct
     6121 gtatttgcca tgctggcgaa cgtctccggc gcaccgttaa tgttaaccgc gctggcactg
     6181 ttgttctcca actcctatgg cggcatggtt actcactatg gcggcgcggc aggtccggtc
     6241 atctttggcg tgggttacaa cgatattaaa tcctggtggt tggtcggtgc ggtactgacg
     6301 atattaacct tcttggtgca tatcaccctc ggcgtgtggt ggtggaatat gctgatcggc
     6361 tggaacatgc tgtaaatata ctcgtcatac ttcaagttgc atgtgctgcg tctgcgttcg
     6421 ctcaccccag tcacttactt atgtaagctc ctggggattc actcacttgt cgccttcctg
     6481 caactcgaat tatttagagt atatccattt attatctttc tgcgcacttc acggtgcgca
     6541 gatatctgga gcatttgatg atcaagttat ctgaaaaagg cgtgtttctc gccagtaata
     6601 acgaaataat tgccgaagaa catttcaccg gcgaaattaa aaaagaagaa gcaaaaaaag
     6661 gcactattgc ctggtctatt ctctcttctc ataatacgtc cggaaatatg gataaactta
     6721 aaattaagtt tgattcatta gcctctcacg atattacctt tgttggtatt gtacagaccg
     6781 ctaaagcgtc cggaatggaa cgtttcccgc tgccgtatgt gctgaccaac tgccataact
     6841 cactctgcgc cgtcggcggc accattaacg gtgatgacca tgtttttggt ttatcggcag
     6901 ctcagcgtta tggcggtatt tttgtgcctc cgcatattgc ggtcatccat caatatatgc
     6961 gtgagatgat ggctggcggg ggcaaaatga tcctcgggtc agacagacac acccgttacg
     7021 gtgcattagg gacaatggca gtcggtgagg gtggcggtga gttggtaaaa cagctgctta
     7081 atgacacctg ggatatcgac tatccgggcg tggttgcggt gcatctgacc ggaaaacctg
     7141 cgccgtatgt ggggccacag gatgtggcgc tggctatcat tggcgcggtg ttcaaaaacg
     7201 gttacgtcaa aaacaaagtc atggagttcg ttggaccggg cgttagcgcg ctctctaccg
     7261 atttccgtaa cagcgttgac gtgatgacca cggaaaccac ctgtttaagc tccgtctggc
     7321 aaactgatga agaagtgcat aactggctgg cgctgcacgg tcgcggtcag gattactgcc
     7381 agcttaaccc tcaaccgatg gcgtactacg atggctgcat cagcgttgat ttaagcgcca
     7441 tcaaaccaat gattgcgctg ccgttccacc cgagcaacgt gtatgaaatc gacacactga
     7501 accagaacct gaccgacatt ctgcgtgaga ttgaaattga gtccgaacgc gtggcgcacg
     7561 gtaaagccaa actctcgctg ctggataaag tggaaaatgg tcgcctgaaa gtgcagcagg
     7621 ggattatcgc gggctgttct ggcggtaact acgaaaacgt catcgcggcg gcgaatgcac
     7681 tgcgcggtca atcctgtggc aatgacacct tctcgctggc ggtttacccg tcatcacagc
     7741 cggtgtttat ggatctcgcc aaaaaaggtg tggtagcaga tttgattggc gcaggcgcaa
     7801 tcatcagaac cgcattctgt ggcccatgct ttggcgcggg cgatacgcca atcaacaacg
     7861 gtttgagtat tcgccacacc acgcgtaact tcccgaaccg cgaaggctct aagccagcca
     7921 atgggcagat gtcagcggtg gcgttgatgg acgctcgttc tatcgctgcg actgcggcaa
     7981 acggtggcta tttaacctct gccagcgaac ttgattgctg ggacaacgtg ccggagtacg
     8041 ccttcgatgt aacgccgtat aaaaaccgtg tttatcaggg ctttgtgaaa ggggcaactc
     8101 agcaaccgct gatttacgga ccgaacatta aagactggcc ggaattgggt gcgctgactg
     8161 acaatatcgt cctgaaagtg tgctcgaaga tcctcgacga agtcaccacc accgacgaac
     8221 tgattccttc cggtgaaacc tcttcttatc gttcaaatcc gattggtctg gcggggaatg
     8281 tcagcgagct gacagaggtg tttgcgcgca ttaagcagat tgctggtcag gagcatattg
     8341 atccgctgca aactgaaatt ggtagcatgg tctatgcggt gaaaccaggc gatggttctg
     8401 cgcgtgaaca ggcggcgagc tgccagcgtg tgattggcgg tctggcgaat attgccgagg
     8461 agtacgcgac taaacgctac cgttctaacg tcatcaactg ggggatgtta ccgctgcaga
     8521 tggcggaagt gccaaccttt gaagtggggg attacattta catccctggc attaaagcgg
     8581 cgctggataa tccgggtacg acgtttaaag gttatgtgat ccatgaagat gcgccggtaa
     8641 cggaaattac gctttacatg gaaagcctga cggctgaaga gcgcgagatt atcaaggcgg
     8701 gtagtttgat taacttcaat aaaaaccgtc agatgtaaaa agcgccatgt gaatgtaggt
     8761 cgcattcggc acttattgtc ggatgcgatg ctggcgcatc ttatccgacc tacaaatcgc
     8821 atcgaaccgt aggccggata aggcgtttac gccgcatccg gcaaatagtt aatagctctt
     8881 acttcttctc ctctgcaacc actttgctgc ccacgccgcg gttattgtat tcccacatgc
     8941 ggttgtagtt agtgtcattc agattgcgct gtatttcgtc gttatcatct acgctgccgg
     9001 tattacccgc aaacggacga ttagagatca ccgcatcggc ccacggttta gccgtgttaa
     9061 aaccttcgtt gatggcgcta tcacggatca ccacctgacc gttggtatta gcatcaacat
     9121 ccagcgagcg gcccagttgc gccacgccat caccggaagc attgaaacgg ctgtttacgg
     9181 cgaggaaacc gtaatagatg ttagacagcg tagccggtgc aaacacatat gcttcttgct
     9241 gggtacgtga gttcaccacg cggaattcgg tgttatcgaa caccactgcg ccgcgaccag
     9301 aaacgatatc cacatcccct tcaatatagc tgttggtcac cagcgtacgc ggctgacggt
     9361 tggtttccag acggttctgc acaccgctgt tggtgacaaa gaaggtgttc tgacgaccga
     9421 gaatgttaac gttgttaatc tgtacttggt caccgtcagt acgcagtgct accgccggat
     9481 ggttacctgc atctacgcta tcgcccagcg tgttttcgat ggtcagattt tgcagttgca
     9541 gtccattgtt ttgtgaccag aagaccgcag aacacagaac accgatactg tcgctgcgtt
     9601 tgctctggca gctatcgtac atataccacg ctggtttacc tggcatatat ttgccgcgcg
     9661 ggttgacgtc gtgacgccag tcggcagggc tcatgccccc atcaagggaa agcccaatct
     9721 tcacatcaat cggtttttca cctgtaccgt acagagtgat tccacctgga gcagcaggga
     9781 catacaccgt tccctgatac tcaccaggca tcacggcaat atactggcgc ttgttggtgc
     9841 gcttgataat tgccgcatct accgccgcct gaatcgtggt atgcgttaca ccttgagtac
     9901 ccgccgggcc gacaacaaag tcaggttgcg caggcagggt tatcggggac ggattccacg
     9961 ctgcagcacc tggtgtcagg gatgcaaaat agtgttgcgc atcgaaattc agcgcttctt
    10021 ttgccgacag aatcgggcga gaagaggtac caggcgcagt ttgatcagaa ggacgttgat
    10081 cgggtggtgt tgagctacag gcggtcagcg tcacgccaaa agccaatgcc agcgccagac
    10141 gggaaactga aaatgtgttc acaggttgct ccgggctatg aaatagaaaa atgaatccgt
    10201 tgaagcctgc ttttttatac taagttggca ttataaaaaa gcattgctta tcaatttgtt
    10261 gcaacgaaca ggtcattatc agtcaaaata aaatcattat ttgatttcaa ttttgtccca
    10321 ctccctgcct ctgtcatcac gatactgtga tgccatggtg tccgacttgt gcccgagaag
    10381 atgttgagca aacttatcgc ttatctgctt ctcatagagt cttgcagaca aactgcgcaa
    10441 ctcgtgaaag gtaggtggat ccccttcgaa ggaaagacct gatgcttttc gtgcgcgcat
    10501 aaaatacctt gatactgtgc cggatgaaag cggttcacga cgagtagatg caattatggt
    10561 ttctccgcca agaatctctt tgcatttatc aagtgtttcc ttcattgata tcccgagagc
    10621 atcaacatgc aatgttgtag ggatggcaat ttttacgcct gttttgcttt gctcgacata
    10681 aagatatcca tctacgatat cagaccactt catttcgcat aaatcaccaa ctcgttgccc
    10741 ggtaacaaca gccagttcca ttgcaagtct gagccaacat ggtgatgatt ctgctgcttg
    10801 ataaattttc aggtattcgt cagccgtaag tcttgatctc cttacctctg attttgctgc
    10861 gcgagtggca gcgaccgggt ttgttgttat atggccttca gctattgcct ctcggaatgc
    10921 atcgctcagt gttgatctga ttaacttggc tgatgccgcc ttgccctcgt ctatgtatcc
    10981 attgagcatt gccgcaattt cttttgtggt gatg
//
LOCUS       DAJTRH010000118        10909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTRH010000118 DAJTRH010000000
VERSION     DAJTRH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            272..1102
                     /locus_tag="OO339_003410"
     CDS             272..1102
                     /locus_tag="OO339_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5927915.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1243..2016)
                     /gene="uxuR"
                     /locus_tag="OO339_003411"
     CDS             complement(1243..2016)
                     /gene="uxuR"
                     /locus_tag="OO339_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5927916.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2231..3691)
                     /locus_tag="OO339_003412"
     CDS             complement(2231..3691)
                     /locus_tag="OO339_003412"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5927917.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3772..4956)
                     /gene="uxuA"
                     /locus_tag="OO339_003413"
     CDS             complement(3772..4956)
                     /gene="uxuA"
                     /locus_tag="OO339_003413"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5927918.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5296..6639
                     /gene="gntP"
                     /locus_tag="OO339_003414"
     CDS             5296..6639
                     /gene="gntP"
                     /locus_tag="OO339_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5927919.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6890..7792)
                     /gene="fimH"
                     /locus_tag="OO339_003415"
     CDS             complement(6890..7792)
                     /gene="fimH"
                     /locus_tag="OO339_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5927920.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7812..8315)
                     /gene="fimG"
                     /locus_tag="OO339_003416"
     CDS             complement(7812..8315)
                     /gene="fimG"
                     /locus_tag="OO339_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5927921.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8328..8858)
                     /gene="fimF"
                     /locus_tag="OO339_003417"
     CDS             complement(8328..8858)
                     /gene="fimF"
                     /locus_tag="OO339_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5927922.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8868..>10909)
                     /gene="fimD"
                     /locus_tag="OO339_003418"
     CDS             complement(8868..>10909)
                     /gene="fimD"
                     /locus_tag="OO339_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5927923.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2633 a         2806 c         2664 g         2806 t
ORIGIN      
        1 aataccattc acaggcatta ttaatataga cctcaatata gcaatatcaa attccaatta
       61 gtaataagat atgcttttaa attgcatgca aataatttta aatgagcgtt ttttgatact
      121 ttagagtgtt tcctaccctt taatatcact gtgtagagtt gatatcacta caacagaaac
      181 atgcatgtca tttgtttact tctcttttta ctacagaaaa atttatctat ttttctaaac
      241 atcagttaaa tactcataaa aggtaactat tatgtctatg ccattaagca atgccgctaa
      301 taaccaattc aatacaaata atcacttcct tcatcatcca aaagtcgaca gtgagttaac
      361 acgtcgatat gaatatgcac gcctggatac ggaaaatatc tatcttctgc ctcttgctcg
      421 tggcaataac cataattacg atggaaaaag cgttgtcgaa attagaaaac ttaatattgg
      481 caaacagtcc tggccattta attatgtgac agatacatgc agagaattca atggtattac
      541 aacaacagga cgtatgctct atcgaaactt gaaaatcacc tcagctttag acgaaatata
      601 tggtggtata tgtaaaaagg cgcatgctgc tacagagtta gcagaaggtt tacggctaaa
      661 tttatttatg aagagccctt tcgatcctgt tgaggactat acagttcatg aaataacatt
      721 aggccctggt tgcaatgttc ctggttatgc cggaaccacg ataggatata tttcaaccct
      781 tccagcttct caggcaaaaa gatggaccaa tgagcagcca agaattgata tctatattga
      841 tcagataata actgtctcag gggtagcaaa ttcttccgga ttcgcccttg ccgcattact
      901 taatgctaat attgagatgg gaaatgaccc aatcattggt atagaagcct atcctggtac
      961 cgcggagatc cattcaaaaa tgggatataa cgttatcccc ggtgatgaag atgcgccact
     1021 caaacgcatg actctgcaac cgtcatctct tccagaactg tttgaattaa aaaatggtga
     1081 atggaattat attggtaaat agccactgag aagaatgagt actaatattc aggttataca
     1141 gatatttaaa caaaaccctg caatttacaa agtaatactc attgcgactt cttaatttaa
     1201 gaaagcgcaa ggaacgttta cccttgcgct tattataata agtcaggcgt cgactttatc
     1261 cagcggccat gaatcaaaga gatagccgtc aaaataaata tcgtcaacgt tcgagaattc
     1321 cagcagacgt tgcttaacgt tttccagatg ctgccacatt gccagcttcg ccgctcgggc
     1381 atcttttttg attaacgcgg cgaggatctg tttgtgatcg cccaaccact ctttgcgata
     1441 caggctgtca tccagatggc tgtgcaactg gatccacatt ggattgtttt cccgccactg
     1501 ccaggactga cggaataact ccaccagcat gctgttatgc gttgcttcag caatggcgag
     1561 atggaactgc atgtcaccgc tttcgctgct acccggcgcg ctggaagcca gttcacgctc
     1621 ttccagttgc aatgcctgac gcattttgac gatatcttcg cgggtagcct gcagtgcggc
     1681 aaactcggcg atgttacttt ccagtaactg acgcgcctgt aacagctcaa aaggacccgc
     1741 atcattgcag acgttggcat ccggactgtc tgtgttgtgg ctgcctgagc tatcaagcac
     1801 atagataccg gcaccccggc gcacttccac cagccctttg atctccagca tgatcagcgc
     1861 ttcacgtacc accgtccgcg tgacatcaag catttctgca atttcacgct ccggcggcag
     1921 ccgttcgcca ggattgtacg gcgtctttat gatcagatcg tggatcatcg ccccgacttc
     1981 ctggtaaggt ctttgcgcag aggtgacaga tttcatggtg ttgtgtccgt taggtgcggt
     2041 taatcaaatt tttgccggat gcggcgtgaa cgccttatcc ggcctacaac gggcaatttt
     2101 acggcccccg ttaatctcaa actataccat tagcactgga agttggcacg tacaggtggg
     2161 caaaaaaacg aaaactggtc aaccaaatat cagtttgatt gaccagtaag tctgtagggt
     2221 aatcagttag ttacagcgca gccacacact cgcgcgcacc gcgttcgcac agctgctgat
     2281 atgccgacgt caccgcgcca acaaagtcgg cattctgcgg cagatcatcg gcaaaaatac
     2341 cgctcaggcc cagcagcgct ttcacgcggt ctgcgccctg atactgcgcg ttaatcttct
     2401 ggaactcggc cagcatcggg tcgaccacgt caatggcatt accctgctca tccacgccct
     2461 gggtgtaacc catccagcca gccacgccca gcgccaggtg acgccagctg ccgccgtttt
     2521 gcaggtgcag acgcaccggg tccagtagac gctgcggtaa cttctggctg ccgtccatcg
     2581 caatctgcca ggtacggtga cgcagagacg ggttgctgaa acgctcgatc agcagcgtcg
     2641 cataggcgtt caggtccgta ccttccggca tcgacagcgt tggcgcttgt tcctgcatca
     2701 tcagggcaaa ggccgctttg cgataatccg ggttagtcat ggtgtcggca atggtttcat
     2761 agccgccgag gtaaccgagg tacgccagga aagagtggct gccgttcagc atacgcagct
     2821 tcatcatttc gaacggcaca acgtctgcaa cgaactgtgt gcccacttta tcccagtccg
     2881 ggcgaccatt aacgaagtta tcttcaatca cccactgacg gaacggttcg caggcaatgg
     2941 cgcacgggtc gtaaacaccc agctggtcag caatttcctg taaggtttct ggcgtcgccg
     3001 ccggaacgat gcggtcaacc atggtgcacg ggaaggtgac gttctcttca atccatgccg
     3061 ctagctgcgg gtcacgcgcc tgagccagcc ccagtaccgc gaccttcgcc acatggccgt
     3121 tttcacgcac gttatcgcag gacatcaccg taaatgcttt cagccctttt tcacgacgca
     3181 gacgcaaggc ttcgacgatg taaccaatcg cggacttcgg cgcagtcggg ttttccagat
     3241 cgtgcttgat cagcgggtta ttgagatcca gttgaccgct tgccgcatca gcgcagtagc
     3301 ctttttccgt gaccgttaga gagacaatcg ccgtttgcgg acgcgccatc gcgttgagaa
     3361 taccttcgca gccgtcgatt tccggatgca gcgcttcttt catcgaaccg ataattttca
     3421 gctcggtgct ttctgcgcct ttttccgcta cggtgtacag cagttgctgt tttttcaggt
     3481 tttcgatcag catgcggtcg ttgcctggca tcaggttaac ttcgcagatg ccccagtcgc
     3541 tgtcggtgct ttccagcaga tgatgggtat acagcgcctg gtgcgcgcgg tgaaacgccc
     3601 cgcaaccgag atgcacaatg cgtgattcca gacgagaatg atcccatgac gggcgggcaa
     3661 ccggcagatt gctgtcaaca atagtagtca tcgtaaactc cagaatccgt cttcagagag
     3721 tgacggcaag gaagaaggaa ccggcacggc gcgcagccat gccggtgggt attaacggat
     3781 aaagaaagcg cgctggatcg ccagttcgac accgcgaact tcggccaggc ctttcagacg
     3841 accaattgcg gagtaaccag ggttggtttt cttcttcagg tcgtccagca tctgatgacc
     3901 gtggtccgga cgcatcggga tcaggtcttc tttgccttcc gctttacgac ggtgttcttc
     3961 ttcaacaatc gctttcacca ctttgtacat atcaacgtca ccgttcaggt gcgccgcttc
     4021 gtggaaggtt ttcgggttat cttcacgcat ggtggagcgc agatgggtga agtaaatacg
     4081 cggaccgaac tgcttgatca tatcaaccag atcgttgtca gcacgcacgc cgtaggaacc
     4141 ggtgcacatg gtgaaaccgt tcgccatgct gtttacggta tcaaccatcc actgcatatc
     4201 ttcaatggtg gaaacaatgc gcggcaagcc gaggatcggg cgcggcggat cgtccgggtg
     4261 aaccgccata cgcacgccaa cttcttcagc aactggaata atcgctttca ggaagacggc
     4321 aaagttttcg cgcagtttgg ctttgtcgat atctttgtac agctccaggt gcttacggaa
     4381 ctggtcgagg gtataccctt cttccgcgcc cggcagaccg gcaatgatgt tacgcgtcag
     4441 acgcgctttg tcttcgtcgc tcatagtggc gaagcgttca gctgcctgag caatttcttc
     4501 ttcggtgtaa tccgcttccg cgcctggacg tttcaggata tgcatttcga atgcagcgaa
     4561 ttcgatctgg tcgaagcgca gagctttgga gccgtctggc agcacgtatt cgaggtcagt
     4621 acgggtccag tcgagcaccg gcatgaagtt gtagcacacg gtgcgaatgc cacactgcgc
     4681 caggttgcgc agagtctgct gatagttagc gatccactgc tcatagttgc cagtgtgggt
     4741 tttgatatct tcgtgaattg gtacgctttc tacgacagac cacaccaggc ctgcgtcttc
     4801 aacgatcgcc ttgcgtttga ggatctcttc tacggaccat acttcgccgt tcgggatatg
     4861 gtgcagcgcg gtaaccacgc cagttgcgcc cgcctgacgg acatcagcta aagaaaccgg
     4921 atcgtttggg ccgtaccagc gccaggtctg ttccatgatt tttcctcttt cacatagtgg
     4981 gttccacagc aaagagacgc ggaaccttgg agttgtttag gtatccggat gaacaaccag
     5041 accgcagccg tggcgttgca tcggatgaga tggcgtataa gttctacctt ttggtaagac
     5101 atgtcaacca gaattctgaa attggttaac cgcgtcgcaa gaatttcact ctcatggttg
     5161 ttgctgcaaa ttgcagcaaa gggtaatcga tggttattga tcagaggatg tgacattcat
     5221 cgcaacaatg gttgaccaat ttacataaca tgtcgcccaa aataacaacg gttcaaccaa
     5281 gtaaggtaaa acgttatgca tgtgcttaac attctctggg tggtattcgg cattggcctg
     5341 atgctgatac tgaatttgaa gttcaaaatc aattcaatgg tggctttgtt ggtggcggcg
     5401 ctgtccgtcg ggatgctggc aggcatggat ttgatgtcgc tgctgcacac catgaaagcg
     5461 ggcttcggca acacgctggg agaactggct atcatcgtgg tgttcggtgc ggtcatcggt
     5521 aaattgatgg tcgactccgg cgcggctcac cagatagcac atacgctgct ggcgcgtctc
     5581 ggtctgcgct atgtacagct gtcggtgatt atcatcggcc ttattttcgg tctggcgatg
     5641 ttctatgaag tggcctttat catgttagcg ccgctggtta ttgttattgc cgccgaagct
     5701 aaaattccgt tcctgaaact ggcgatcccg gcagtagcag ctgccactac cgcacattca
     5761 ctgttcccac cgcagccggg tccggtggcg ctggtgaatg cttatggcgc ggatatgggg
     5821 atggtttata tctatggcgt actggtgacg atcccaagtg taatctgcgc aggtctgatc
     5881 ctgccgaagt tcctcggcaa tcttgagcgc ccaacgccat cattcctgaa agcagatcaa
     5941 ccggtagata tgaataatct gccctctttc ggcgtttcga ttctggtgcc gctgatcccg
     6001 gcgatcatta tgatctctac caccatcgcc aatatctggc tggtaaaaga tacccctgcc
     6061 tgggaagtgg ttaactttat tggttcctcg ccgattgcga tgtttattgc gatggtggtt
     6121 gcattcgtac tctttggcac cgcgcgtggt catgacatgc agtgggtgat gaacgctttt
     6181 gaaagcgcgg tgaagagtat tgcaatggtg attctgatca tcggtgcggg tggcgtgctg
     6241 aagcagacca tcatcgacac cggcattggc gacaccatcg gcatgttgat gtcccacggc
     6301 aatatctcgc cctacattat ggcatggctg atcactgtgc taattcgtct ggcgacgggt
     6361 cagggtgtcg tttcggcgat gaccgccgcc gggattatca gtgctgcaat ccttgatcca
     6421 gcaaccggtc agctggttgg cgtgaatccg gcgctgctgg tactggcgac ggctgcgggt
     6481 tccaacaccc tcacccacat taatgatgca tctttctggt tgttcaaagg ttactttgac
     6541 ctgtcagtaa aagacacgtt gaaaacctgg ggtctgctgg agctggtcaa ctccgtggtt
     6601 gggctgatta ttgtgctgat tattagcatg gtagcgtaaa agaacaccag agcctgccaa
     6661 tggcaggctc agactgatga ctaaagtcta aaaatgtgcc cggacagtcc cctcgcccct
     6721 ccggggagag ggttagggtg aggggaacag accagtaaag tgcgctttgt ttatgccaga
     6781 tgcgacgctg acgcgtctta tctggcctac aaagggctaa cgtgcaggtt ttgtaggtct
     6841 gatagcgtag cgcctcaggt accagcatta gcaatgcgct gtaatttttt tattgataaa
     6901 caaaagtcac gccaataatc gattgcacat tccctgcagt cacctgccct ccggtacgtg
     6961 cgtaatttgc cgttaatccc agacttaccg ccgaagttcc tactgctcct aacgataccg
     7021 tgttattcgc tggaataatc gtaccgttgc gcgtcaactg tacgccgacg ccctgcgctg
     7081 gtgaaaacga cgcggtattg gtgaaaatcg agttgcccgc atctgcggtt gtgccggaga
     7141 ggtaataccc caggttttgg cttttcgcac aataaacggt aagaggaatt ggcactgaac
     7201 cagggtagtc cggcagagta acggtgacat cacgagcaga aacatcacaa ccgccagtgg
     7261 gcaccaccac atcattattg gcgtaaatat tccacacaaa ctgaaaatca tcgctgttat
     7321 agttgttggt ctgtcgcaaa ataagcacgg caattaatga accagcttta atagccaccc
     7381 cgcccgcact gctcacaggc gtcaaataaa gcgccaccgg ccacggctta tccgttctcg
     7441 aattataaac aacccgcggc gtttcgctgg tggtcggaaa tggatagcta ctgccactat
     7501 attttacggt cccggaaaaa ttagataaca cgccgccgta agccgagcct cgttgcagtg
     7561 tgacatagtc tgtaatggtt tccggataat cgttatggca aaagatttgc gtcgaaagat
     7621 ctacgaccag gttttgcccc acattcacga caggcgcaag gtttacataa acattagcgc
     7681 tgccaccgcc aatagggata gcggtaccat tggcggtttt acaggcgaat gaccaggcat
     7741 ttaccgacca gcccatcagc agtacagcaa acagggtaat agctcgtttc attacaatta
     7801 tctcttcggg ttcagctgta ggtataggtg atgctaatca ctgcctgaat ggttccctga
     7861 gtggctccgc catttaccgt caatgctctg acctgtaacg ggaagtgcgc tgattgtgag
     7921 gaatcatcca cctgaactgt tttggttgcg ccagtattca atgtgttgcc actgtcatcc
     7981 tgtagctcta actggatgtt ttgcgcggtc ccctggtttt tataatatcc ggtgctgtcg
     8041 gctgccccgc tgaagctggc agtgaccctc gacgttccca ccggacaatt agtcaactca
     8101 agcgcaacat catgccaggc cgatgccgcc ccggcagaca taagactgaa agaataaaga
     8161 tcgccgagat ctaccgtggc attggtggtg gaaaccgtac acggtttggc gacgacctta
     8221 ccgttcaccg tgatggtgac atcggctgcc tgtatcgtcg cacttgcgag cgccaacatt
     8281 gccgccaata catacccacg tttgcaccat ttcatgagca tctccagtta ctgatattca
     8341 agagtgaagg tagccgtagc attgatatgc cccgcagtga caggcacctg tgtcgccatt
     8401 agccgggcgt aaaaattcag cgtatttggt ttacccggcg tcagggtcgt ccacgaaatc
     8461 gcagacgatg gagcattaag gggtatttga ttttgctgct cattcagaag ctgtattccc
     8521 agtcccgcag ccgctgacac cgtattttca agtgcaagca ggttggcatt gtggctatct
     8581 gcaacgccgg taaacccaac ttttacggca gaaacggcat taccacaggg tgacagcaaa
     8641 atacgaaatg gaacgacagg agtcgtcgcg ccaatgttgt taaattgctt cgccgcgttt
     8701 tccatcagat caacagtaaa attggttgat tcagcggcca cactacagcc gttatctctg
     8761 acatagccgc ggatagtaat cgtgctatcc gcagccaaaa cgcgacttac cgccagccac
     8821 aaaaaagcgc acagaagata aaaaggtttg tttctcatca cgccccctta acgacattca
     8881 gctgatagct gggttaataa ctgctgctga ctctctggtg gcagttgata attggcgaca
     8941 cagtgagcat tttcctcttc tccccatttc acctgaactt ttcccgctaa aggcattccg
     9001 ctgaggtaaa cctgaccatt atccgcaaca atgccgctac tctggctact ctctgatgtc
     9061 accatcgccc caaacggcag cggcttatta ttgtgagtta gcgtcatgag cagttttatc
     9121 ccaacgcgcg ctttaaactc tgctcgcacg atcgccccac gagtgggaac aacgttagcg
     9181 accgcgttat ctaaatcgac gttatcagcc aaggtattgg tatccagcgc cactctattt
     9241 tcccgatatt cagtggcata aggcagcacg gcataaccac gccagtcggt acgcaccccc
     9301 gtctggtttt cgacttttgc atcttttgcg ccaggcgctt taacaagcac caccgtatcg
     9361 tttaacggct gccccagcgt tacgccattg gcatgagcca ataccccacc gctgactccg
     9421 taatagagct gcttaatatc atcgctatgg ctgtaaccga tattggcatt gccgtaacca
     9481 ccgcgataat tcagcgtggc gtagcctgta cttccgctat taccatcgcc tcccccggca
     9541 tagccggttt gcacgctata actgaggttg ttgtcttcca gcaacgtacc gtatacacca
     9601 gccagattgg tcatccgacc gttgagatcg tgtgacatgc tgtagctggc actggcatgt
     9661 cgccactgag atttactgtc agaacgcagc cagtggctga aaggaatatt gacgttaagc
     9721 gctaacatct gatcccgtcc tttttgccag gcgtttttcg tcaggctata gctgagcgtc
     9781 cagttgatat cttcgaacgc agtatttaat ccagcctgga attgctcatc gacattactc
     9841 gttccccaat aagtttgatg gctaccactc aaatacagtg ttgatgtgcg cccgagttgc
     9901 tgagtaacgg tgagctgtaa tttcccgcgt ttgttataag cgaggttgta atagtcggtg
     9961 aatttcggct taacctgaat aactccatcc tgtgtttcga tgttgtagcc attcattcga
    10021 ctgtatgttg tatcagcgaa attaaaatat ccgctggtcg aataacggta acccactaac
    10081 tgaatattcg tgcctgattc attgagcgat ttgttataga gaaaacgcac cgattgtccg
    10141 tcatgctgac tgtcatcggg aagtgtggaa ttagcctgcg tcatatccac agacagagcg
    10201 cccagtgcct ccatgttttt cccgataccg aaattaaaag cacgataacg atccgccagt
    10261 tgcgttccac catatattgt ccagccagcc ggaaggccgt ggagtaatgt actctggaaa
    10321 aagcggggtt tttcctgctg cgcatttcca ctacggtatt ctcctgccgt aatggaataa
    10381 cgagtatgcc cttcacgttg caaaagtggg actgacgaat agggtacggt aaaaatctgc
    10441 gtgctgccgt cagcctcttt gatcgttacc tgcaagtcac cactattacc tgcggcatag
    10501 atatcgttga tggtaaaagg ccccggtggc accgtactat tataaatgcc atatccattt
    10561 tgtttaatag tgacctgtgc agtaccacga gcaataccgt ggatcaccgg ggcaaatcct
    10621 ctttggctat cgggtaacat attgtcatct gaggccaatt gtgcgccgcg aaagttaata
    10681 ccatcgaaaa tatcgccctg ggtataacca tcacccaacg tcagccggga acgtaaagga
    10741 attatgtctc gctcaatcca ggtaatgata tgctgccatt tatttttgct acctgatgaa
    10801 ctgtcgctac tgttataact ccaggtggta ttgtcgcgta aacgccacgc accaatattt
    10861 aacccactct gtaggtttaa atatgcataa tggctgttac ccccaatcc
//
LOCUS       DAJTRH010000119        10880 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTRH010000119 DAJTRH010000000
VERSION     DAJTRH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..204
                     /locus_tag="OO339_003419"
     CDS             <1..204
                     /locus_tag="OO339_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927924.1"
                     /translation="FGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIA
                     IYLLAGCLISAMTALLMKDNQRS"
     gene            306..1760
                     /gene="amn"
                     /locus_tag="OO339_003420"
     CDS             306..1760
                     /gene="amn"
                     /locus_tag="OO339_003420"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5927925.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2103..2819
                     /locus_tag="OO339_003421"
     CDS             2103..2819
                     /locus_tag="OO339_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5927926.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3275..3350)
                     /locus_tag="OO339_003422"
     tRNA            complement(3275..3350)
                     /locus_tag="OO339_003422"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3315..3317),aa:Asn,seq:gtt)
     gene            complement(3451..5078)
                     /gene="yeeO"
                     /locus_tag="OO339_003423"
                     /pseudo
     CDS             complement(3451..5078)
                     /gene="yeeO"
                     /locus_tag="OO339_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5083..5158
                     /locus_tag="OO339_003424"
     tRNA            5083..5158
                     /locus_tag="OO339_003424"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5116..5118,aa:Asn,seq:gtt)
     gene            complement(5196..6146)
                     /gene="cbl"
                     /locus_tag="OO339_003425"
     CDS             complement(5196..6146)
                     /gene="cbl"
                     /locus_tag="OO339_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5927927.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6248..7165)
                     /gene="nac"
                     /locus_tag="OO339_003426"
     CDS             complement(6248..7165)
                     /gene="nac"
                     /locus_tag="OO339_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5927928.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7493..7568
                     /locus_tag="OO339_003427"
     tRNA            7493..7568
                     /locus_tag="OO339_003427"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7526..7528,aa:Asn,seq:gtt)
     gene            complement(7624..8559)
                     /gene="ldtA"
                     /locus_tag="OO339_003428"
     CDS             complement(7624..8559)
                     /gene="ldtA"
                     /locus_tag="OO339_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5927929.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8621..9700)
                     /gene="cobT"
                     /locus_tag="OO339_003429"
     CDS             complement(8621..9700)
                     /gene="cobT"
                     /locus_tag="OO339_003429"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5927930.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9712..10455)
                     /gene="cobS"
                     /locus_tag="OO339_003430"
     CDS             complement(9712..10455)
                     /gene="cobS"
                     /locus_tag="OO339_003430"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5927931.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10452..>10880)
                     /gene="cobU"
                     /locus_tag="OO339_003431"
     CDS             complement(10452..>10880)
                     /gene="cobU"
                     /locus_tag="OO339_003431"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5927932.1"
                     /translation="MAARIEHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECV
                     TTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGI
                     VPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3020 a         2770 c         2354 g         2736 t
ORIGIN      
        1 tttggtgcca gttatcgcta cagtggtgct ggagtcggtt atcaggttgc cagtgtggtt
       61 ggcggtggat ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg
      121 catagcatcg ccatttattt gctggctgga tgcctgattt ctgcaatgac cgctttattg
      181 atgaaagaca atcaacgctc gtgatagcct ggcgaagatc atccgatctt cgccttatac
      241 ttttgtttca cattcctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga
      301 cacacatgaa taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg
      361 cgctgtatga gcaatctgta gttgctttac gcaacgccat tggcaaatat attacaattg
      421 gcgaattacc tgatgaaaac gcccgtaaac aaggtctttt tgtctatcca tcactgaccg
      481 taacctggga cggtagcaca acaaatcccc ccaaaacgcg cgcatttggt cgttttactc
      541 acgcaggcag ctacaccacc acgattactc gccctactct ctttcgttcg tatctgaatg
      601 agcaactaac gttgctttat caggattatg gtgcgcatat ctctgtgcaa ccctcgcaac
      661 atgaaatccc ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga
      721 gcgctgattt aactcgctac ttcccgacaa cagaactggc gcaaatcggc gatgaaactg
      781 cagacggcat ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg
      841 tcgatttttc cctcgcacgg ttgcgccatt ataccggtac gccagtagaa cattttcaac
      901 cgttcgtctt gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca
      961 gccagatcct cgatcctgat agtccctaca ttgccctttc ttgtgctggc gggaactgga
     1021 tcactgccga aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc
     1081 agatgccagc atggcattta attaccgctg atggtcaggg tattactctg gtgaatattg
     1141 gcgtgggacc gtccaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggata
     1201 tctggttgat gattggtcac tgtggcggat tacgtgaaag ccaggccatt ggagattatg
     1261 tccttgcaca cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata
     1321 tacctattcc gagcattgct gaagtgcaac gtgcgcttta tgacgctacc aagttagtca
     1381 gcggcaggcc tggtgaggaa gtcaaacagc ggctgcgtac aggtactgtg gtaaccacag
     1441 atgacaggaa ctgggagtta cgttactcag cttctgcact tcgttttaac ttaagccggg
     1501 ccgtagcaat tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc
     1561 catacgggac actactgtgt gtttcagata aaccgttaca tggtgagatt aagcttcccg
     1621 gccaggctaa ccgtttttat gaaggcgcta tttccgaaca cctgcaaatt ggcattcggg
     1681 cgatcgattt gctgcgcgca gaaggcgacc gactgcattc acgtaaatta cgaaccttta
     1741 atgagccgcc gttccgataa taagttatga accttcttat cagaaataga tgtgaaggat
     1801 taagtaagac atctggcaaa tagcctgcaa taaacgcggg ctgcttgctt ccttgggcag
     1861 atgcgagttt tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa
     1921 acacaattat aaaaaatccc gccaacaaca ttagttttta taaaattaaa tataagatta
     1981 tggctttaga atatttttat ttctaataga cgagattttt cctgttatga tataatatgc
     2041 cgaattaaca catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc
     2101 cagtgggacg taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt
     2161 ctaaaattta tgcaaaattc ggtgtagaaa tctatgctgc tgccaaacaa ggtgaacccg
     2221 atccagaatt aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc
     2281 caaagcacgt tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg
     2341 tacagggacg ttatgaaggc tttggtccta atggctcaat gattattgct gagacattga
     2401 cttcaaatgt taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca
     2461 atatcggagc ggcaggttct gtcagctata tgtttgacaa tacgggtgtg attgtattta
     2521 aaggaacaga ccctgaccat atttttgaaa ttttgcttga agctgaagtt gatgttcgtg
     2581 atgtaactga agaagaaggt aatattgtta tttatactga acctgctgac cttcataaag
     2641 gaatcgcggc gctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga
     2701 ttgctcaatc tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg
     2761 atgcccttga agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat
     2821 tatattttaa agaaatctgt ctttatggca gatttcttta atctcatata attcttataa
     2881 aaaatataat atttaactcg tcatattgct tataaaaaat ccccgtcccc agagaaataa
     2941 tatttattaa aatggcaggt cttctttttc tgattaccga agacaaagtg gcaattacgt
     3001 atagtttccc gataaagacg cgacagctac gtcccgcata aggcattttt ctctttatct
     3061 ttgtacggta cttcatggaa cagaattttt gaccttgtga atcgtgatat ctgttgggga
     3121 ggggcaattt gctcactgaa gcgtgagact cgattaagcg cacgaaacac agaaatcaaa
     3181 aaacccggcc acttttttac aaagtggccg ggtaaaaata atttttattt tttaactgtc
     3241 ctgagtctca tagagatgtc tcaaaactaa aatttggctc ctctgactgg actcgaacca
     3301 gtgacatacg gattaacagt ccgccgttct accgactgaa ctacagagga atcgtgagaa
     3361 cgaggcgaat attagcgatg cccacccaca atgtcaaagc ctgtttttta aatttgaaat
     3421 cgtttgctga aataatctgc attttgtcgt ttattccgac acaactggct ttttttcaca
     3481 cttttgcggc tcggatcgag ggtatttcca cagccaacgt ccagtaacca ttcgccagta
     3541 aaacagcacg gcccgcacag cccagtcggc aaacattccc atccagacac caaccacacc
     3601 ccagccaagc atgattccct gcacataacc gactacaacc cggcaacccc acatgctcaa
     3661 catagaaacc cacatggcgt aacgggcatc acgagccccc ttaaatccag cgggtagcac
     3721 ccatgaggcg gaccaaatag gcataaataa agcatttagc taaatcagaa tcacaacgac
     3781 gtgtttaacc tgtggatcct gggtgtaaaa cgatgccata accccggcaa agggagccgt
     3841 tagccaggcg atggccgtta atccaagagt ggacagccag aacacatgcc gcaactgaat
     3901 ctctgcttgc gctatctgcc ctacccccaa ccttcggcct gtaatgatcg tagaagcaga
     3961 accgagcgca cttccgggta agttgataag agccgcaatt gagaacgcga taaaatttcc
     4021 ggcaataacg ctggtcccca tcccggcaac gaacatttgg gttaataacc gaccactggt
     4081 aaataacact gattcgacac tcgctggaat accaatcccc atgacttccc agataatgct
     4141 aaaattcagc ggtttaaaat agctctttaa cgaaatcctt agcgcaggat taaaaccaat
     4201 cgccagcacc cacaaaattg caactgcgcc aatataacga gaaatggtta aacccaaccc
     4261 agccccgacc aggagaaaag gccgtaaatc aatatgccgc taataataat attaagaata
     4321 ttcaggctac cgttaatcag tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg
     4381 ctaccaataa gagtgatggc agcggctgga taactgagta cggtcagctc cagataagtc
     4441 aacgccagtg ctttaacttc tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg
     4501 ccaaaatgat gaattagcgt tgccaacagt acggcaaaca acgtcatgat caccaatgac
     4561 tgccgcgtcg ccaccctcgc tcgtcgtcga tcccgcttac cgagactaaa tgctacaacg
     4621 acagtagtac caagatcgat agctgcaaaa aaagccataa tgaccatatt gaagctgtcc
     4681 gccaacccca cgccggccat cgcatctttt cccagccaac tgaccagaaa agtgctcagg
     4741 acccccatca acaggacaca ggcgttctcc atgaagatag gaacagcaag cggggttatc
     4801 tcgcgctaga acaacacttt gtagctcttg cgtttagagt gccagcgagt gccgtgaaca
     4861 acctggcgta aagcagagga gatattcaaa gccgacctta attgcagaaa gtgaaaccac
     4921 atttcaaatg atgagggaga atcagcaaag ctgcaaagat tttcgccaac aaattgtctg
     4981 caaatgcaac aaactgttga tagaaacggc aaacagttgg gaaatttaaa aatcgggttt
     5041 gacaaaatat ttttcgccgt taagatgtgc ctcaacagcg attcctctgt agttcagtcg
     5101 gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa
     5161 tttctgaaaa gcccgctttt atagcgggct ttttgctata tctgataatc aatttcctct
     5221 tcactgtttt ccatcacctg gcgcttgata tcctcaactg acagtcctgc attacaaagt
     5281 tccagaaagc gccaaacata gttacgctga agttgtcctc gcttcagtcc caaccagaca
     5341 gtattagcat cgaaaagatg ccgcgtatcc aggcggatta aattctcttc ctcttgttcg
     5401 ccgctggatt gttcggcaac taatccgatc ccaagcccaa gagcaacata ggttttaatg
     5461 acatcagaat cctgtgcact taatacaata tctgccggca aacctttgcg ggtaaatgcg
     5521 tcatcagtgc gtgagcgccc cgtaattccc tgtcggtaag taattaacgg ccacttcgct
     5581 attgattcca gcgtcaatgg tgtaatttgc gttaagggat gatcgagtgg aacaagcaaa
     5641 ctatggtgcc aacaaaacca tgggaaggcg acgagctgcg ggtcattact caaacgctcg
     5701 ctggcgatac caatatcagc ttcgccattt tgcaacaacg tcgcaatttc ctgtggcgaa
     5761 ccctggatta gctcgagccg aacctccggg aaaagttcgc gaaaagcttt aatgacctct
     5821 ggcaagctat aacgtgcctg agtatgcgtc gttgcaatag tgagaacacc agacgtatcg
     5881 ttggtaaaca ggtctgcaag ccgacgaaca ttactggctt cattcagaat acgttctgca
     5941 atgaccagta atgctttgcc cggttcagtc atccccagca gtcgcttacc tcgtcgaaca
     6001 aatatttcga tgccaaattc atcctccagt tcccgaatat gacggctaac gcctgactgt
     6061 gaggtaaaaa gcatattcgc aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg
     6121 attatcttta gttgttggaa attcacggta aactccgggc agttcagata tcccgttatt
     6181 gttaaagtct aatgcccggc ataacaaata ataaaaaccc gcatcttatt ccatcccgat
     6241 ataacgctta gctcaccaat tgccactgcc ttttttccat cactggagaa ctaatcactg
     6301 acattaacaa ctctttcact gcctgtgcct gtggtgacaa gtttgctctg gcgggtaaat
     6361 ttaaggacaa agagagactc attgaaggag tggtaatgcg tgacatccac ccatttactg
     6421 cgccacataa cgaacgcgca gccgattcag gtaatactgc aacgcccatg ccgctggcaa
     6481 tcgctgcggt aagcgtggca atagactcaa tttcaccaat gacttttgcc gtgagccgcc
     6541 gtagggaaaa agcctcatca acacgaagtc taacagcgct gtaatcactg gggagaaaaa
     6601 ggttcatttg cgcaatagca ttaacatcaa cgttttgccc cgggcaatct tgagttccta
     6661 ccaggaaaag atcttctttc agcaaagcct gactggatac cccagccaca ggggaatgct
     6721 cataaatcac cgccatatcg agttggtgat ttatcagttt ttcgttaagc actgcaccac
     6781 tattttcatg aagatagata acgacctccg ggaattcagc gcgaaccgcc tgtaataagg
     6841 gcatggtgat ggatgacgca gcggttcctg gcgcaaagcc aatcgagact tgccccgata
     6901 atgactgacc aacgttatgc accgccagtt gggcctgctc acactgacgt aaaatggccc
     6961 gtgcatgggt atagagaatt tttccggcgt ctgttggcgt aacgccccgt tttgtgcgaa
     7021 tcaaaagctg ttgatttaac tcaccttcca gtatggcaac ctgttggctg agcgctggtt
     7081 gtgcgatatg caatacttca gcagcctggg tcaggctacc aatatctaca atttttacga
     7141 agtatttcag gcgtctgaag ttcatgttgc ctccggtttt taagaatcgg cccaagtgcc
     7201 gccattactt acaaccagta tgcaagatgc ctgccagttc tattttggcg ttgatgcgca
     7261 agctaaccaa ctgtcaaata agagattatg atagattcgt cattggccct ttttatcagc
     7321 agtcgcattc acctgcccta taaaaggagg gtatgcacca cgatggttca ttacccaata
     7381 agattgaaag ctcaccactt tgttgaaatt ggcagcaaac aaacaaaaaa aatgcatttc
     7441 accctttgac atcaccatgc actgccatta atatgcgccc cgttcacacg attcctctgt
     7501 agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag
     7561 aggagccaaa ttcaaaaaag cctgctttcg agcaggcttt ttactttcta attaccaacg
     7621 ctcttaaaac atctgtcttg aaccagaact aatttgcaca ggcattcccg atcgatgttg
     7681 caacgcagca tttgcgcgat ttacatcaac ttcttgcccg ttgataaacg cccgcaaaga
     7741 tggggttacc ggcaatggca cttttcggtc agactcatat tctgcacgat tgcgcgacag
     7801 cggctcatga acttccagcc actttgaacc atccggttcc gttgtgtatt tcactggctg
     7861 atcaataatt tgtacacgag tgcctacagg aacattatca aacagatatt tgatatcgtc
     7921 attacgcaga cgaatacagc cctgacttac acggagccca ataccaaaat tggcattggt
     7981 accgtggatg gcatacaacc tgccaatata aatcgcgtac agccccatgg ggttatcggg
     8041 ccccgcagga acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc gccggatgtt
     8101 cggcgttggc gtccaggttg gtgcttcttg tttacgttca acggtagtca cccagttacg
     8161 cggggtttct cgcccagcct ggccgatacc aataggaaag acttccactg tattactgtc
     8221 tggcggatag taataaagac gcatttcagc gacgttaacc acaatccctt tacgaacagt
     8281 ggcgggcaaa atcagttgct gcggaatggt gagttgtgag ccaggcttcg gcaaaaaaac
     8341 atcagcgccc ggattcgctt ccagcatatt acttaaccct tgcccgtatt gtgcggcaaa
     8401 agtctccagc ggctgagtat tgtgatcagg aacagttaca gtaagcgact gccccactaa
     8461 acggctaccc tctggaggta atggataagt taccgccagg ctattttggc tggcaaaaag
     8521 cagagcaaat gagcaaagaa tatttacacg acgcatcatg tccctttcct aagtcgcgaa
     8581 tactatccga taagtatagc ttttatcaga cttttcgttt ttagctgttc aaatcagaag
     8641 tcgtattccc cggtagaaca atattactgg cagcaagttc gcccatgttg ttgtatatcg
     8701 cacaagcagc ttcgatgatg gacatcgcca gagctgcacc actcccctca cctaaacgca
     8761 tatccatatt gagataaggc tccagcccca aatgcgagag cgctatacgc gcgccttttt
     8821 ctgccgacaa gtgagaagga atgagatacg gtttgattgc aggagacatc tggcaggctg
     8881 cgagcgcagc agcataagaa agaaatccat ccagcaacac gggtaaaccg caggaagcag
     8941 cacctaacat cactccagct attccgacca aatcaaatcc acccactttt gccaggacat
     9001 caacaccatc ctgaggattt ggttgattca acgtaatcgc ccgacgcaca acatcaattt
     9061 tattagccag tttatctgtc ggcaggtttg cgccaatccc aaccacttct tcaggatccc
     9121 ggccagtgat tgtgctgact attgccgctg ccggtgtcgt gtttgccatc cccagttcac
     9181 ctacaccaaa cagcgtgaca ccgtttttcg ccagctcccg cgtataacat atgacgtcca
     9241 aaagcaactt ttcagcctga cggcgactca ttgccggagc tgaagcaata ttgccgctac
     9301 ctcgtgcgac acgcatgttg ataagcccgg ggataggctc agcagtatca ataccaacat
     9361 caattacgtg gacgttagcg cccgcttgtg ctgccagcac acacacgccg gttgttccac
     9421 gggtcatatt ttcagcctgt atggctgtca cttcttttgg agaaatagcg accccttcct
     9481 cccagacgcc gtgatcggca cacataacca gtaccgcttt tttgcccaca tgcggtatgc
     9541 cattcacccc cggcattcct gccagttgta tggcaagcgc ctccagcttt cctaggctac
     9601 caacaggttt gaataacccg tcaacatgcc gttgtgcacg cgacatagcg gcagaattga
     9661 tggctgggat cgtattcagt aaatcggcaa ttatttgcat gtcacgtttc tttatagcag
     9721 tgccagcagg aagaccagtt caccaagctc tatggctgct cccagcgtat cgcccgtttg
     9781 cccgcccagc gtacgtttga gaagctgacc gaggatgaaa atcgccacca tcgtcaccac
     9841 catagcagcc acaccatgca tacccagcaa taatacggca gcaaaaatag cggctaagcc
     9901 gagtgtgacg caggtttgtc gcccatcaat tttgccaatg aacacattgc caagcccttc
     9961 ttcccgcgca taacgatgac gatacatcaa taatgcggca gtcccgcggc tgaccgcaca
    10021 tgctgccgcc agcgatgcaa ggatcggttc gccacgcaga gccaactcgc ttaacaccag
    10081 aatctttgcc agtaccacaa aaatcaatgc cagaccgccg tgggtgccta aacggctatc
    10141 acgcatgatt tccaacatgc gatcgcgact acgtgcagaa aatacgccat cacaggtatc
    10201 agcgaggccg tccagatgga attccccggt catcagcgcc agcacaagta cgctaaacag
    10261 tgccgccagt ggtgcgccac accatgcctg cagcaccatg aagaccagcc cgctaatcgc
    10321 gccaagcaat aatccaatca aaggaaaagt aataataccg cgagaataat gttcgaaatc
    10381 cagtccctgg gaccagcgac gcggtacagg cagacgcgta ataaatgaga gcatcgccca
    10441 aaataatttg ctcatttgat ttttactcca atacccgaaa ccaccagcca tacttcattt
    10501 gccgcagcgg ccaactgctg atttacccgc ccggcaatat cacgaaaatg tcgtgccaga
    10561 cgactctccg gcacaatccc cattcccact tcgttagtca ctaatacaac ctttgcgggg
    10621 caacgttggc aggcagcaat caacgactga atctcagcat taatcgcctg ttccatcgcc
    10681 tgataatccc attcatcagg gtctttatcg ccgccataat caaacaacag attagtcacc
    10741 attgtggtaa cgcattccag caacacagcc tcattcgggt taatgtctgc atgaattaat
    10801 tcatcaagat gttgccagcg ctccactgtg cgccagtgct ccgggcggct ttgccgatga
    10861 tgttctatcc gtgcagccat
//
LOCUS       DAJTRH010000120        10629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTRH010000120 DAJTRH010000000
VERSION     DAJTRH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            154..504
                     /locus_tag="OO339_003432"
     CDS             154..504
                     /locus_tag="OO339_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5927933.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OO339_003433"
     CDS             557..952
                     /locus_tag="OO339_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5927934.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OO339_003434"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OO339_003434"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5927935.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OO339_003435"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OO339_003435"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5927936.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO339_003436"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO339_003436"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5927937.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OO339_003437"
     CDS             complement(5323..6423)
                     /locus_tag="OO339_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5927938.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO339_003438"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO339_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5927939.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="OO339_003439"
     CDS             complement(7571..8137)
                     /locus_tag="OO339_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5927940.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="OO339_003440"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="OO339_003440"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5927941.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..>10629
                     /locus_tag="OO339_003441"
     CDS             10263..>10629
                     /locus_tag="OO339_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5927942.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLS"
BASE COUNT         2585 a         2720 c         2555 g         2769 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtg
//
LOCUS       DAJTRH010000121        10158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTRH010000121 DAJTRH010000000
VERSION     DAJTRH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..380
                     /locus_tag="OO339_003442"
     CDS             <1..380
                     /locus_tag="OO339_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCR5927943.1"
                     /translation="GWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVGVTGLT
                     VAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKGVKQGS
                     VSIVGMTSDGEFVAVAAVTVSAP"
     gene            439..825
                     /locus_tag="OO339_003443"
     CDS             439..825
                     /locus_tag="OO339_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5927944.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            834..1163
                     /locus_tag="OO339_003444"
     CDS             834..1163
                     /locus_tag="OO339_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5927945.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1135..4176
                     /locus_tag="OO339_003445"
     CDS             1135..4176
                     /locus_tag="OO339_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5927946.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4176..4505
                     /locus_tag="OO339_003446"
     CDS             4176..4505
                     /locus_tag="OO339_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5927947.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4505..5203
                     /locus_tag="OO339_003447"
     CDS             4505..5203
                     /locus_tag="OO339_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5927948.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5208..5951
                     /locus_tag="OO339_003448"
     CDS             5208..5951
                     /locus_tag="OO339_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5927949.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            5888..6490
                     /locus_tag="OO339_003449"
     CDS             5888..6490
                     /locus_tag="OO339_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5927950.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6551..10030
                     /locus_tag="OO339_003450"
     CDS             6551..10030
                     /locus_tag="OO339_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5927951.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10098..>10158
                     /locus_tag="OO339_003451"
     CDS             10098..>10158
                     /locus_tag="OO339_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HCR5927952.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
BASE COUNT         2384 a         2404 c         3272 g         2098 t
ORIGIN      
        1 gcggctggct gagctcactg ggtaaaacca ttgcctcaaa agacgtgatg acccgcactg
       61 tgaaaatcag cggtgtgggg cgtccgtatc tggcagagga aggcactgaa accgtgggtg
      121 ttaccggact gacggtggca ccggcatctg tcagtgtcaa agcgggagca accaccacac
      181 tgacctttac agtaaaacct gacggggcca gtgacaaagc gatcagtgtg cattcgtcag
      241 atcctcagac tgcttcggtg accctgagcg ggcttgtggc cacggtgaaa ggcgtgaagc
      301 agggcagtgt cagcatcgtg ggcatgacct ctgacggaga gtttgtggca gtggctgcgg
      361 tgaccgtcag cgcaccataa caggacgata ctcatcattg ccccggttat ccggggcttt
      421 ttgcatccgg agaacatgat gtttctgaaa caggatacgt ttaattatga aaaacagtcc
      481 gtggtgctca gtgagctgtc cgggctgcag agaattgaat atctgacgtt tgttcagcag
      541 cgaacggcaa agtttgatgc acaggaggga gaactgccgg aggctgaacg acagattgct
      601 tttctgcgta tgggaatgga tatcaatgcc tggctggttt cccgctcact gtggaatgct
      661 gagcagtctc aggatgttga gacgctttgc gcatccatta tgacaacatg gtcgtatgat
      721 gcgctgggcg cgggggcgga gagggttctg tcgctgagcg gtatggggac cattgagaat
      781 gccggggatg atgatcatga ggcgctgacg ccggaaaagt cctgacgcgg gaaatgcagt
      841 ttgtcatgcg gcttgcccgg gagttccggc gggcagactg gcggcggatg ctgtcggata
      901 tgtcggccac tgagcttggt gagtggggcg attatttccg gatgcagagc ttcagtgatg
      961 tgtggatgga tgcgcagttt gcctcgctga aggcattgat cgtgagaatg gtgtccggca
     1021 gcagcgatgc tgcggtggct gatttcagcc ttttaccgga agagaacggg ataccggagc
     1081 gaacggacga agaactgatg catcttgggg aaggtatttc cggaggtgtg cgttatggac
     1141 cagatagcca acctggtcat tgatttgggg attgatgcgg cagagtttaa aaatgaaatt
     1201 ccccgtatca aaaaccttct gaatggtgca gccagcgatg cagaacggtc ttctgcccgt
     1261 atgcagcgtt ttatggagcg tcagactcag gcggcccggc agacaatgca ggcggcttct
     1321 tcggctgcaa cagcagcatc agcccatgcg cagacggtgg agaagaatgc acgggctcat
     1381 gaacgcatgg cccgtgaggt ggaacaaacc cgtctgcgcg tggatgccct gaatcagaaa
     1441 atgcgcgagg aacaggcgca ggccagggca ctggcggagg cgcaggataa agcggctgcc
     1501 gccttttatc gccagattga cagtgtgaaa caggccggtg cggggcttca ggaattacag
     1561 cgtattcagc agcagatccg acaggccaga aacagtggcg gggttggtca gcaggattat
     1621 ctggcgctga tttcggagat cacggcgaaa acccgtgccc tgacgcaggc agaggaacag
     1681 gccacccggc agaaagcggc gtttatccgc cagcttaaag agcaggcaac ccgccagaat
     1741 ctgtcgtctt ctgagttgct tcgcgccagg gcggctcagc tgggggtaag cagtgctgca
     1801 gaagtgtata tccgcaaaat ggagcgggca ggaaaagcca cacattcgct ggggctgaaa
     1861 agtgcggcag cccggcggga gctgggggtg ttaatcagtc agatggcgcg aggcaatttt
     1921 ggtgcgctga ggggatccgg gataacgctg gctaaccgtg ccggatggac aggcgcactg
     1981 atgtcgccga aaggcatgat gactggcggc gttattggcg gacttgtcgc ggcggtcctg
     2041 ggtctgggta aagcctggca tgacggccgg aaggagggcg agaaatttaa ccgtcagctg
     2101 gcgctgacgg gacattatgc cggtgtcact gtcgggcagt tgtggaaact cagccgggcc
     2161 atatccggga atggtatcac gcaacatgcg gcagccggtg cgctggcaca ggtggtgggc
     2221 agtggggcat ttcatggaaa cgatattggt atggtggcga aagctgccgc acagatggag
     2281 cgatcggttg gccagtcggt cagcgatacc ataaatcagt ttaagcggct gaaggatgat
     2341 cctgtaaatg ccgcgaaggc gctggacaat gcgctgcatt ttttgaatgc cactcagctt
     2401 gagcagatac gcgtccttgg ggagcagggg cggtccagtg atgcggcccg gatcgccatg
     2461 tctgcgctgg cagaggaaac cggtaaacgc acatctgata ttgataataa tctcaatgcg
     2521 ctgggtagta cgctgcaaac cttgtctgac tggtggaagc agttctggga tgcggccatg
     2581 aatattggtc gtgaagactc gctggatgcg cagattgatg cgttacagga aaaaattcag
     2641 cgcgcgaaaa aatatccgtg gacaaacgcc tccacacagg tggagtacga tcagcagcgt
     2701 cttaacgatc ttcaggagaa aaaacgccgg aaggatttgc aggatgcaaa agcgcaggca
     2761 gaacggaatt accaggagca acagaaacgc cggaatgctg aaaatgccgc gctgaaccgg
     2821 atgaatgaaa cggaagctgc acgacatcag cgggaaattg cgcgtattaa tgccatgcag
     2881 tacgccgacc aggctgtcag ggatgcggcg atacagcgtg aaaatgaacg ttacgaaaaa
     2941 gccattaaga aaaatacacg ggcaacccgt aatgatgagg ccacccggtt attgctgcag
     3001 tacagtcagc agcaggcaca ggtggaaggg cagattgctg ctgccagaca gtcagcaggc
     3061 attgccactg aaaggatgac tgaagcgcat aaacagcttc tggccctgca gcagcgcatc
     3121 agcgacctgg acgggaaaaa gctgacggca gatgaaaaga gtgtgctggc ccgtaagaat
     3181 gagctgattc aggcgctgac gctgctggat gtgaaacagc aggagctgca gaaacagaca
     3241 gcgcttaacg acctgagaaa aaaaacggtt cagctgacca gccagctggc agacaaagaa
     3301 cgtgcactgc gtgagcagca caatctggat attgccactg caggtatggg ggataagcag
     3361 cggcagcgct accaggcaca gttgcgcatc cggcaggaat accggcaaca gttgcaacag
     3421 cttgagaatg acagtcgcca gaaaggcact tacgggacgg aggactaccg gagggctgag
     3481 gaggtgctga aggggagcct gaagcgacaa ctgaatgaaa acaaacgcta ctggcaggaa
     3541 ctggaagtgg cgcagggcga ctggaaaaac ggtgccatgc gggcgtttca gaattttacg
     3601 gcggatgcgg ataatgcggc gggaacggca gaacagatgt ttacagtggc attcagcagt
     3661 gccggtaatg cactggcgac attctgtacc accggtaagc tgaattttaa atccttcacc
     3721 tcttccctgt tgtcagatat ggccagaatt atggcacaga tggccatgat gcaggcggta
     3781 aagggcgtcg gttctttatt cggcttcacg actaatgctg atggcggtgt ttaccagtct
     3841 gctgatttga gtcgctacag tggcacggtg gttaaccgtc cgacgttttt tgcttttgca
     3901 aaaggcgcgg gtgtgatggg ggaagcggga cctgaagcca ttctgccact gcgtcgcggt
     3961 gctgatggta agctgggggt tgtggcggat actggtggtt caggcatggt gatgtttgcc
     4021 ccgcagtaca acattgagat caataacgac ggcacgaacg ggcagatagg tccggctgcc
     4081 ctgaaggtgg tttatgacct cgggaaaaaa gcggccgcgg actttatgca acagcaggcc
     4141 cgtgatggtg gccggttaag tggagcatac cggtaatgga gacgtttcac tggaaagtgc
     4201 gcccggatat gaatgtggta tcagagccga aagtggtgac agtgaagctg ggcgatggtt
     4261 atgaacagcg tcgtgcggcg ggactgaata accagttgtc gacttacagc gtgacgatac
     4321 gtgttcgtaa aggtgaacac ccatctttaa aagcctttct ggaacggcac ggtggcgtcc
     4381 gcgcatttca gtggacgcca ccttatgact ggaaactgat cagggtggtt tgtcgtaaat
     4441 ggtcggcaag cgtgggggcg ttatgggtga ctgtaacggc cgattttgaa caggtggtga
     4501 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcagc
     4561 ccgggtggag ctctgggaaa tcgatctgac agaggtcggt ggtgagcgtt attttttctg
     4621 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcggcagt atcaggcata
     4681 ccccattcag gggtcggggt ttgaactgaa cggcaggggc tgtgctgccc gtccgacact
     4741 gacggtctct aacctgcacg gcatggtcac cgggatggcg gaagatctgc agagtctggt
     4801 cggcggaacg gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt
     4861 caacggaaac agcgacgccg atccggaaca ggaggtgatc agccgctggc gcatcgagca
     4921 gtgcagcgaa ctgagcgcgg tcagtgcctc ttttgtgctg gccacaccaa cggagacgga
     4981 tggcgcggtt ttcccggggc gtatcatgct ggcgaatacc tgtatgtgga cttaccgttc
     5041 tgatgagtgt ggttacacgg gcagggcagt ggctgacgag ttcgacaaac caacaacgga
     5101 tatccggaag gacaaatgca gcaagtgtat gcgcgggtgt gagttgcgca acaataccgg
     5161 taatttcggc ggtttccttt ccatcaataa actttctcag taaatccatg acacagacag
     5221 aatcagcgat tctggcgcac gcccggcgat gtgcgccagc ggagtcgtgt ggcttcgtgg
     5281 tgagaacgcc ggagggagac aggtatcttc ccagcgagaa tatctccggt gagccggagg
     5341 aacggttccg gatggctccg gaggactggc tgcgggcaca aatgcagggt gagattgtgg
     5401 cactggtcca cagtcatccc ggtggtctgc cctggctgag tgaggctgac cggcggctgc
     5461 aggtgcagag tgatttgccg tggtggctgg tctgccgggg gacgattcac aagttccgct
     5521 gtgtgccgca tctcaccggg cggcgctttg agcacggggt gacggactgt tacacgctgt
     5581 tccgggatgc ttatcatctg gcggggattg agatgccgga ttttcatcgc ggggatgact
     5641 ggtggcgtca cggtcagaat ctctatctgg ataatctgga ggccacaggg ctgtatcagg
     5701 tgccgttgtc atcagcacaa ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc
     5761 cgaatcatgc cgccatttac tgcggcgatg gtgagctgct gcaccatatt cctgaacaac
     5821 tgagtaaacg agagaggtat accgacaaat ggcagcgacg cacacactcc ctctggcgtc
     5881 accgggaatg gcacgcatct gcctttacgg ggatttgcaa cgatttggcc gccgcatcga
     5941 ccttcgtgtg aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggcgtt
     6001 tcgtcagaaa ctgagtgacg gctggtatca ggtgcgcatt gccgggcgtg atgcaggtga
     6061 aaccgaattg tctgcccgtc ttaatgagcc gctggcaaat ggtgcagtga tccacatagt
     6121 accgcgtctg gtgggagcta aaagtggcgg tgtgtttcag gcggtgctgg gggcagctgt
     6181 tatggcggtt gctatatgga tgccgggggt aggaattatg gcgagtaatc tgctgttttc
     6241 tctcggtgcc agtatgacgc ttggcggtgt tgcacagatg ctggcaccga aagccagaac
     6301 tccccgtaca cagacaacgg ataacggcaa acagaacacc tgtttctcct cactggataa
     6361 catggttgcc cagggcaatg ttctgcctgt tctgtacggt gaaatgcgcg tggggtcgcg
     6421 ggtggtatct caggagatca gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat
     6481 tggtcgctga tgcaaaatgt ttcatgtgaa accgcctgcg ggcggttttg tcgtttatgg
     6541 agcgtgagga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
     6601 cctgaaatcc acgcagttgc tgagtgtgat tgatgccatc agcgaagggc cggttgacgg
     6661 tccggtggat ggattaaaaa gcgtgctgct gaacggtacg ccggtcctgg atagcgaggg
     6721 gaagacaaac ttttccggtg ttacggtggt gttccgcgcc ggcgagcagg agcagacacc
     6781 gccggagggg tttgaatctt ccggctcaga gactgtgctg ggtacggaag tgaaatacga
     6841 cacgccgatc acccggacca tcacgtcggc aaacattgat cgtctgcgcc tgaccttcgg
     6901 tgtgcaggca ctggtggaaa ccacctcaaa gggggaccgg aatccgtcgg aagtccgcct
     6961 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
     7021 caaaaccact tcgcagtatc tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt
     7081 taatatccgg atgcgcagga tgacaccgga cagcaccaca gaccagctgc agaacaaaac
     7141 gctctggtcg tcgtacaccg aaatcatcga tgtgaaacag tgctacccga acacggcgct
     7201 ggtcggcgtg caggtggatt cagagcagtt cggtaaccag caggtgagtc gcaattatca
     7261 tcttcgcggg cgcattctgc aggtgccgtc gaactataac ccgcagacgc ggcaatacag
     7321 cggtatctgg gacggaacgc ttaagccagc atacagcaac aacatggcct ggtgtctgtg
     7381 ggacatgctc actcatccgc gctacggcat ggggaaacgt cttggtgcgg cagatgtgga
     7441 caaatgggcg ctgtatgtca tcggccagta ctgcgaccag tcagtgccgg acggatttgg
     7501 cggcacggag ccgcgcatca cctgtaacgc ttacctgacc acacagcgta aggcgtggga
     7561 tgttctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
     7621 gacgttcgtg caggaccgac cgtcggataa agtgtggacc tataaccgca gtaatgtggt
     7681 gatgccggat gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa
     7741 tgtcgttgag gtgaactgga ttgatccgga caacggtcat gagacggcga cagagcttgt
     7801 ggaggacacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
     7861 ctgtaccagc cggggacagg cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct
     7921 ggaaacgcag accgtggact tcagcgtcgg cgcagaaggg cttcgccatg taccgggtga
     7981 tgtcattgaa atctgcgatg atgattatgc cggtatcagc accggtggtc gcgtactggc
     8041 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc cgtcctccgg
     8101 taccacgctg ataagcctgg ttgacggaaa tggcaatccg gtcagcgtgg aggttcagtc
     8161 cgtcaccgac ggcgtgaagg tgaaagtgag ccgggttcct gacggcgttg cagaatacag
     8221 cgtgtggggg ctgaagctgc cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg
     8281 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaggc
     8341 catcgtggat aacggggcac actttgacgg tgaccggcgc ggcacggtga atggtgtcac
     8401 gccgccagcg gtgcagcatc tgaccgccga agtcaccgca gacagcgggg aatatcaggt
     8461 gctggcgcga tgggacacgc cgaaggtggt gaagggtgtg agcttcctgc ttcgcctgac
     8521 cgtggcagcg gacgacggca gtgagcggct ggtcagcaca gccaggacga cggaaaccac
     8581 ataccgcttc aggcagctgg cgctggggaa ttacagtctg acagtccggg cggtaaatgc
     8641 ccgggggcag cagggcgatc cggcgtcggt atcgttccgg attgcggcac cggcagcgcc
     8701 tgtcactatt gaactgatac cggggtattt tcagataacg gtggtcccga aactggctgt
     8761 atatgacccg acggtgcagt ttgagttctg gttttcggaa aagcggattg cggatatcag
     8821 gcaggttgaa accagcgccc gctatcttgg cacggcgctg tactggatag ccgccagtat
     8881 caatatcagg ccgggccatg attattattt ttacgttcgc agtgtgaaca ccgttggcaa
     8941 atcggcattt gtggaggccg tcggtcgggc gagcgatgat gcggaaggtt atctgagttt
     9001 ttataaaggg ttgatcaata aaacgcatct cggcaaggag ttgtggacgc agattgataa
     9061 cggtcagctt gcgccggacc tgactgaaat caggacgtcc attacgaatg tcagcaatga
     9121 aatcacgcaa accgtcaata aaaaactgga aaatcagagt gctgcaatcc agcagataca
     9181 gaaagttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcaaca
     9241 gatgaaggac ggacgccttt atattgcggg tatcggtgcc ggtattgaga atacgccagc
     9301 aggaatgcag agtcaggtgc tgctggcggc agacaggatt gcgatgatta atcctgcgaa
     9361 tggcaacaca aagccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
     9421 cctgaaacgc ctgacggctc cgaccattac cagcggcggt aatcctccgg cattttccct
     9481 gacacctggc ggacggctga cggcgaaaaa tgccgatatc agcggtaacg tgaacgcgaa
     9541 ctccgggacg ctcaacaacg tcacgattaa taagaactgt cgggttctgg gaaaattgtc
     9601 cgcgaaccag attgaaggcg atctcgttaa aacagtgggc aaacctttcc cacgggactc
     9661 ccgggcaccg gagaggtggc catcagggac cattaccgtc agggtttatg acgatcagcc
     9721 gtttgatcgg caaattgtta ttcccgcggt ggcgtttcgc ggtgctaaac atgagcggaa
     9781 gaataacaat atttattcgt catgccgcct gatagtgaag aaaaacggtg ctgaaattta
     9841 taaccgaacg accctggata atacgctgat atatacgggt gttattgata tgcctgccgg
     9901 tcacggtcac atgacgctgg agttttctgt atcggcatgg ctggtaaatg gctggtatcc
     9961 cacagcaagt atcagcgatt tgctggttgt tgtgatgaag aaatccacag caggtatcag
    10021 tatcagctga attttataac ccagatacgg gcaccagaaa tggtgccttt tttattgcag
    10081 aaaagcgaga ggtaattatg cgtaaacttt atgctgccat tttgtccgca gccatctgtc
    10141 tggctgtatc cggtgcgc
//
LOCUS       DAJTRH010000122        10156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTRH010000122 DAJTRH010000000
VERSION     DAJTRH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(150..712)
                     /locus_tag="OO339_003452"
                     /pseudo
     CDS             complement(150..712)
                     /locus_tag="OO339_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(916..1122)
                     /locus_tag="OO339_003453"
     CDS             complement(916..1122)
                     /locus_tag="OO339_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5927953.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1229..1792)
                     /locus_tag="OO339_003454"
     CDS             complement(1229..1792)
                     /locus_tag="OO339_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5927954.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3167..5002)
                     /locus_tag="OO339_003455"
     CDS             complement(3167..5002)
                     /locus_tag="OO339_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927955.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5103..5390
                     /locus_tag="OO339_003456"
     CDS             5103..5390
                     /locus_tag="OO339_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5927956.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5362..6891
                     /locus_tag="OO339_003457"
     CDS             5362..6891
                     /locus_tag="OO339_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5927957.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7061..8278)
                     /locus_tag="OO339_003458"
     CDS             complement(7061..8278)
                     /locus_tag="OO339_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5927958.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8645..9631)
                     /locus_tag="OO339_003459"
     CDS             complement(8645..9631)
                     /locus_tag="OO339_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5927959.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9628..10156)
                     /locus_tag="OO339_003460"
                     /pseudo
     CDS             complement(9628..10156)
                     /locus_tag="OO339_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3163 a         2324 c         1903 g         2766 t
ORIGIN      
        1 attaatttaa actgtaataa tttaatttta ggatgtgtag cagcagcccg atacagagtt
       61 gcaaggacac acttttgcca gagggcgtta caggaaagct taatgtttga ttctgtatac
      121 ataataaatc accttatagt tacaataggt caaaaaccgc tgtagccaaa gttacgctgg
      181 cctgatgctt tagtgtcgag cttcgtcaaa taatccagac gtaccaataa gcgctggtac
      241 tgctctggga aattaggatc gttaatatcc aggatgtaac gtccattagc agggaaatga
      301 actaatccct ggtgatcttc cagttccaac cctaaggcac tgtaccaggc tccggtaatt
      361 aaccctctca gggtgtcaga gcgctcatag tttcccagat ggtaataggc gtctttgtta
      421 aaaaaaggca gacgtgataa tgacatttaa aggactgcgt atattctttc gcccagataa
      481 tacaaaccga cgttgcgtag gtgcgtttgc cttgttgttt tttacggtgt tgatctgcat
      541 taattttcgc attcagcgag ttacggaaac gagatatctc tccggggatc aggttaggga
      601 aacaggtaat gatatccccg ttgtcaatca caggagggta atggagatcc ccccgtattg
      661 ctgttattct ggaagtgttt ttaatattcg tattgatagt tctcagagat atatcacact
      721 ctagatattt taattaagta tcagttcgat atcggcatat tgtacactac ctcaggtaga
      781 tgatgagatc agatacaggg atcaactgac ggtgagcgca gtccccacat tttatccagg
      841 gcttactgca tattcccaac agccattcgt tagccaggta gacgaatacc ctgatttgcc
      901 gctggttttg ctttcctatc gaagggccca tacatcatca cgcccacgaa acaaccgacg
      961 aaataacgca actttctcat ccgtggataa tacgaaaacg cactgcacag ggctctgcgg
     1021 tttacgtcgc cattcaatcc catgagcttc aagtaatgag accagccggg tattttctgc
     1081 ccgcagtact gctagttcat cgctgtcaga tttataaacc atatgcgtta tcacttgtac
     1141 ctgtctcccg gcggcaccac gactgaaatt cgtgtgaagg caaatccacg cgataactta
     1201 cgatagttcg gacataagcc cccataattc agttatgtcg tggtcgaccc cggttacttt
     1261 tttcgggaag cctgctacgg cccagaataa taccgtgttc tttctgatcc tgtttagcca
     1321 gatagctaag ggcgtaacgt aagtcgtcta ccgcagattt atcactatag tgaatcacgt
     1381 gatcgatacg caccgggtat ttgtctttag cccggcacag atgaaaataa ccctcccctt
     1441 ccgtgatccg cctccagata tctcccagtt gcctggatat ctgataagag ttcttgtgac
     1501 gttgtccatc caggtagccg ataaaatgaa tatgaagccc tttgtctggt gtatattcca
     1561 ttacccagta atatcccgcc aacatcgtct gggtttcgct aagtaaacgg tatatttcca
     1621 tacacatact gtgcttacag gaatgcccga aactgggcgt gtctttcctg taagcaaaat
     1681 caattctgaa aggtaacagt ttagaaaaac gttgaaacat accatccatg tgttcattca
     1741 cgtctttcag aatcatgaaa tccattttgt aattgggatt ggcattatac attttatagt
     1801 ccttacttta taaaactcac aggaagagag aattacgtaa atactgagag tagagaaacg
     1861 ccgtaccacg gcattaataa acgaagtaaa ccagcagcac agacaacctg ataaaactgt
     1921 tttgtattac cgcattactg tgatgaggta atacttcaga atacagtgag actctcttaa
     1981 actgagaacc agtacgaatt acaatggaaa aacacacctc atagaattga gtttcaggaa
     2041 gcaccactaa attctttaca ggaagtaatc tgacattcac aaaactcagt atcggctagc
     2101 tacaggcaac atcgctatta atattgtcga accattaagt aaagccgaga gagaccacgg
     2161 gtatttaatc attagcataa aagaataatg gtatacagaa catacccaca caccataaga
     2221 agattgttcc atacagaaaa gatcatattt acctatacct ataaactgta acggagtcat
     2281 aacacgctct aatagtgttc cgatacgtaa ccagtgttaa gtacgtacag gacaacatta
     2341 cacacataat tcaccataca gcttccccca tatgcttcac tgcgtagtgt atggagatat
     2401 atgcattaca cacatacaga atacttagca taaagaggag tgacattacc acgtaaagag
     2461 ataagttata caacaaacat acaaacaata cggcacagac acacaatacc ataactccaa
     2521 ctaataatac taattaacct taattattta tggtatatga aataaatgtg attctcccgg
     2581 aactaactaa cctaaccaga cagatgatca ctgtataaaa acttcagtta atactcattt
     2641 gttttgtcga ctcagtatat aatgagtgac cagtaatcag gataatcaca cttacataac
     2701 ctgtaaaaaa gaacaagaag aataagccac taacataatg caaaatggca tctgaatcga
     2761 tcgcagtttg gcattcgaat cgatcatgat ttggcccaat ccgtgatcga atagagcgga
     2821 atacgcaaca taattcacca gacaagagct tacaccgaat tacagattat gtattaatgt
     2881 caaaactggc atattttaca gatacaatga acatgcaacc catcaatcta ataggggcgg
     2941 tatgtacatc tgcccatgaa tgggatggcg ctaagaatac aaaacaatta gacatagatc
     3001 acaaaaacac accattctaa ttgaaaaaca aaacaaacaa ataaatcata ctattataga
     3061 attttctctt gccagacata gataataccc ctgctaattt tgctaatggt tagcaaaatt
     3121 agcagggtat ataaagatta ttgacccaaa acacactcag atgaattcac gacagctcag
     3181 gagggttatt cctgatgtca tcttcacaaa taatggttaa cgcatcatat atcattctgc
     3241 gtctgttcct gttttcggcc ttatcgccat acaacatcat cacattttta atttcatcga
     3301 ccggtgattt cacatagtga actaatacac caagcttctc aacacattgc agtaaagtat
     3361 atttcttact gcctgtgaaa agccctatca tagtttcttt acgttcgcga ctggataata
     3421 ctttgttttc cataatgact tctgccagcg tcttaccatc ataaacatac tctttatcta
     3481 tcagaatatt tatcttatcc agcaccttct ctacgtcatc aagcaaacct gtaatatatg
     3541 ggggcgcaat atatggtttc gtactgatct tttcaagcat acaattatac atcttaataa
     3601 cacgcatcag ggtaaaattg ttcgcatcat actgtatttc accctcctta tatacaggat
     3661 caataagtgg tattttttca ataaatgcat cctgataggc ttttacatgg tcaagccaat
     3721 aaaacaacga gccatacttt atattttttc tatttacttt aacctccagt gctgtccgaa
     3781 gcaacgataa tgcccccctg acatatcctg aaggtaattc accaaacaat ccttttcttt
     3841 ccatgtcatc ataatgctgc agcacatcat taattttatt tttccttatc agacaaatgt
     3901 acaccgcaag tgtttcgtag cagctggtca catgaacagc ctcattctgc aactgcccaa
     3961 aatagcttat aatctttttc agatatgcgt catcacaacc attcttatca ttaatattaa
     4021 atacaagatc tttcagacgg gaaatatatt cctgagattt atcatcaagt gaaacactat
     4081 aacgagacaa tgttccttct gcactgtttt gaaacttata aaacatttcc agtaattttt
     4141 ttgcataagg cgttgtatca aagctggtaa agtcataaag atattgcgca gcgtaaccat
     4201 acaaaccatg aatccagagt ccttctctca ccaacactct gagataatat gcaaggcttt
     4261 gaagtatctt cgtctttcgt tgtatatcaa gtaagacact taatgaataa aatgcattat
     4321 agaatttatc aatattcctg agcactttct ccagttcatc cttatccagc ttcttctccc
     4381 ggtaatcctt tttatattca tttttgatag ccatgatata ttgttgcatc aggctcttgc
     4441 ttttcccgtt atccttccgt atagttcctg tatatttccc cagagcatac agctttccat
     4501 tttcatgaaa atcttccttg ttgaatccca gttcagccag atattcccga agataacgtt
     4561 taacagcagc ttcatcgctt tctgtatcag cgtgatcaag acaaggacaa tacgatttct
     4621 gtaaaaaatt ataaatatga taataaaaag cagagtcatt ctctggtctg tatgtattat
     4681 acatatctac aacaagaaaa ataaccccaa aagtgcgcag acaatagctg cgaaggaatt
     4741 caggttttag tgtggaatgc actggataag aattcatcat gtaataaaaa tgttcatatc
     4801 gggcatatat gatccgccgt tgctcctgac tgaacacccc ttcagcatca ataatacgac
     4861 tgatctcatt ataacgttga tcccagaatt tttgatgcat ttcaaaattg gtactcttcc
     4921 gcttgtgtgc cggattctcg ccttccttaa acaaccccaa aacagtacaa atatcataca
     4981 acatatttgt aaatgtgcgc atatgctgtg ctctccggtt ggtttgcatt ttatcgcaga
     5041 ggacattctg agcttaacgt atcaaactga cacaacgaca tgcaatgcat atggagaaca
     5101 atatgtcaaa tcaaaccgga cagctttaca cgccttcacg catcctgcgt atggctgaac
     5161 ttacccgact gctgggtata tcaagatcta ctatttacgt gaaaataaat ccggcctcga
     5221 aataccacga ccctaccttt cccaaaccaa taaggcttgg tagtgcatcc gtaggctggc
     5281 gggcatctgc catcgatgag tggctgatgt tacatacggc accagcctgg tctgaacctg
     5341 aaaacaaact aaaggaactt aatgatgacg acaaaactat cactccctga acttgacaca
     5401 actaaactgc ctgacaggtg ccaggcatta cttgatgaaa tgcatgaaga aacaggaata
     5461 cgccgtgaaa tacttctgtc tgttatgctg acggtcatgg ctgcctcggt ccaggatacg
     5521 catgaagttg aactttccgg agggcaacgt accagccttc agatatttat gtgtctttca
     5581 tcagcatcag gcagtggaaa aacctctgcc tgcgcaaaat taatcgctcc tgttcatgaa
     5641 acagaagaag aactacatca ggcatatatc gacgataaaa aaaactatga ccgaatgatg
     5701 gaaatgtgga caacagataa aaaaatcctg gagcggagat ataaaaagga aattgaaaga
     5761 tccccggaaa atgctgctgc tgcacgagca gcacttgaaa agtgtatagc aaataaaccc
     5821 gaacctcccg tacaacaggt tctcatcgtg aatgacgcga caccagaagg gatagctttg
     5881 aaactcagcc agtcaccctc tcttctgttg ctgtctgatg aaggaggtac tattctggat
     5941 aagcgtttcg aacgcaaatc ggcactgtac aacacattat ggagtgggca accagtcaac
     6001 gttgaacggg catccagacc cgggttccgg ctgaatgatg tccgtctcac tatacttata
     6061 ctgactcaac cggtaatatt taataagttt tttactctca ctggcgatca gatccggggc
     6121 aatggctttc tggcccggtt gctattttgc gaacccggag caattaaaat aatgacgaca
     6181 gaaccgcata ctgccactcc tgttgtgacg caacaatgtg caagctgttt tcgtaagaaa
     6241 agttttggca gtcagatcag agactctctg agagcatcca gagaaagacg tgcaaaaagt
     6301 aaagagcgca tctgcatgac attatcaaat gcagcatccc gcgcactgga gcgctttcac
     6361 aaagaaaata tggatactgt cagacagaat cctcatatgg gtacatttga agacattatt
     6421 gtcagaaaaa gagagcaggt tgtcaggata gcagcacttc tggaactgga gaaagatcca
     6481 gactcgacag taataacgct ggaaagtact aatagcgcca tttatctcat tgaattttat
     6541 ttcaagcatc ttatatataa acttgaatcc cttcgggaaa tatccccggc tgagaaactt
     6601 gataaatggc ttcagaaaag aattatcaca acagcaggtt acatattcca gaaaagttat
     6661 attttacaat atgccccata tgctctcagg aaaaaatgtg ttctggatga agcacttgac
     6721 atactggcgg aacaaaggaa aataaggatt gatgataacc tggttgttta tataggaaac
     6781 acgattacac catccgaact ggcaaaaaaa ctaaatattc ctgcattcga tgccggagtt
     6841 ttcatttgtg atcatcaaaa catactgaag tatcaccgaa acagactata gatactatcg
     6901 gacaaaaaat aaattatcac aagtgtatta ttcgttataa taatgaataa tcgttatctg
     6961 agtgcaatgc caggttttca ttacgaactg gcattgcatt tatttttcaa aaaagaaagc
     7021 agtaaccatt tggctactct gagtatgcca gtccatgaaa ctaagcgaat tccttagtgt
     7081 ttttagcaag ttgagacatc acataatccg cccatgcctg catcatcggt acacgctgct
     7141 ccagtagatc agtacgatga tatgccgcct caaccttatt tttcagcgta tgagcgagcg
     7201 ccctttccgc cagatcccgc gaatacccct gttcactaca ccagtccctg aatgttgagc
     7261 gaaaaccatg tgccgtggca actcgccccg gaatgtcact gacggctttc tttttacgca
     7321 gaaaacttgt caacaccata tcggaaagga tctgctgctt tctgggtgaa gggaacacca
     7381 gttcatcatg caggccacgt atattttcca gaatgtaaat agcctgccgg gataaaggaa
     7441 cacgatgctg tagcctagct ttcattcttt ctgcaggtat agtccatacc cgcttatgaa
     7501 aatcaatttc agcccagcgc attcccctgg cttcgcccga gcgagttgct gtaagtatca
     7561 ccattaataa cagtgcgcgg gtaacattat aaggttcatc actatacaca ctggtcgcca
     7621 caaaaagcgg taactgcctc cagggcattg cgggttggtg ttcatcacgt cctcttgtct
     7681 gctgaggaag caaatggtca accacatcaa caggatttgc cacacaaaaa ccgtgcgccc
     7741 atccccactg cataacaaca tgaatgcgct gtttaacccg gcttgccgtt tctgacaagg
     7801 ttaaccagac tggacgcagt gtttctgcca cgtccgcagc cgtaatcgaa tccagcgttt
     7861 ttgctcccag ttgaggaaac gcgtaattct caagcgtcga taaccactgc cttacatgct
     7921 ttggattctc ccatccagga gacagttctg catgtacacg cctggctgca tcggcaaatg
     7981 ttgggataac gactttctca gattcagcct ttttaatctc cagaggatca tcacctgcag
     8041 caagttgctc tcgcattatc cgggcagtac gtgcagcttc agcaatactg acctctgggt
     8101 aagttcccaa tccagcatta cgtctttttt gtgtcaccgg acttacataa cgaaaaaccc
     8161 atttcccccg cccctttact gaagaaggat gaagggtcag tccggtaatt cccccatggg
     8221 gcaatggttt gtcatcaggt ttgatatgtc ttgctttcgt atccgtcaat actgccatac
     8281 gctaattccc gtcactctgg tatgccatcc agtatgccat tagtccctcg cttcactaag
     8341 aatacattag ataacatcgg acaacaaaaa gtgtaataca ttgtttataa agaaattata
     8401 taacacccct ggattaagtc agatttattt caggcggtcc ccctcaccgc catatttaaa
     8461 gaagagcgcg tacgaaagta cgggcttttt tttcgtatat tgtacacacc gggtgccccc
     8521 gcccacgtct gttagggcga ggggaaattg tgcacatcaa ctgtctggtt actcagtcaa
     8581 caatcaaaat gcgtaagata gtacagaaca tatatgcgtt atctggtcac ttcctgtaac
     8641 ttcctcactt attaaaatgc ccccaacgga aatacatgtc acatgccatc ataaaatcgt
     8701 tatgccagag gaatcgacaa tggaaaaaat agtaaattaa gcacggtaca tagcagagta
     8761 aaaaataggc ttcaaaccaa atccgccatt ccacaagcgt tgccaatacc gacaccagcg
     8821 taatccacat catttcaata acccgcaata tgaataaccg aagccaaagc ggcttggcaa
     8881 aaaaggtcga caacgtattt tcactggaga aaaacagatc gatttcctgc aaaaaaaacc
     8941 tgcccagaaa agcgggccgc ttggagaagc gcagaaaaaa cagattaatc gccaacacta
     9001 cgccacaaag aatgagcaat gcaattatcg atttaaacgt taaccactgc ccctctgtca
     9061 caaaagaatc actgttacgc agcgggctga tagtaagcag gttaacaacc gttgagatgc
     9121 acaccgaaag cagcacatag cgagtcagac ttttatattt tacttttttc gctactgaaa
     9181 tagcgacctg cttgacataa ctggtgcgga tcatccgaaa cagcagaaag atgaccaacg
     9241 gcccaagtaa cgaaatcagg aacagcgcat ataacgaggg tagaagctgg tatagccccc
     9301 aactcattgc actcaacatg attaacgaca gaaatcctgc caccagaggt tgtgtaagaa
     9361 ataattctat tttgtggttc tgctttttct taaaccacga aaaatcgata ttcttcgtga
     9421 cgcatttata agcccagtac gccttgagat caaaaataaa attatacagc accagggcca
     9481 gaatcccact aactacacca atatcctctg atggaaaagt aaaccctgca tttttccaat
     9541 aaaagcctat cagaataagt ccaatagcat aaatgtatat cagatttcgt ttatcctgaa
     9601 agcgaataaa agttaaataa tcaggaatca tttgaatacc tttgcatcac taaatgcttt
     9661 acgacaatgc ataactcgtt gctgatccac gccagcaaaa tcatttaaca tattccccaa
     9721 atagggctta ttacttattt caaggaaagc taccggggtt ggcatattca gaatatgtat
     9781 cgagagattc agtccatttc accgtatgag ttaagtgtaa tgagagattt tctttgataa
     9841 ttgacggagc gacctcgctt tttgctgtta cgttcatcag aacctgatgc tctggcgaag
     9901 cgatatccag tccggcaaga taatcgcgca ttgcctgaac gccgtcttcc atcaaacgcg
     9961 tgtgccaggc accgctcaca ccgagtttaa ccggttcgta tccagcggcc atcagcagcg
    10021 tggtaaattc attcaacgca gcctgcgttc ccccaataac ctgctggcgc ggcgtgttat
    10081 cacagctaat atccagcgca atgcctgatt ccgtaatcat cgtctgcagt tgttcgcggt
    10141 tgatgccttt aaccgc
//
LOCUS       DAJTRH010000123        10055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTRH010000123 DAJTRH010000000
VERSION     DAJTRH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..198
                     /locus_tag="OO339_003461"
     CDS             <1..198
                     /locus_tag="OO339_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5927960.1"
                     /translation="LPRGAFFISALFFFNAVCIVSDNLLIIESFGKMAYNISYLTRVP
                     GTNTLLACCCLSRPDEVNSEY"
     gene            188..361
                     /locus_tag="OO339_003462"
     CDS             188..361
                     /locus_tag="OO339_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5927961.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(410..1483)
                     /locus_tag="OO339_003463"
     CDS             complement(410..1483)
                     /locus_tag="OO339_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5927962.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1585..4296)
                     /gene="torS"
                     /locus_tag="OO339_003464"
                     /pseudo
     CDS             complement(1585..4296)
                     /gene="torS"
                     /locus_tag="OO339_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4379..5407
                     /gene="torT"
                     /locus_tag="OO339_003465"
     CDS             4379..5407
                     /gene="torT"
                     /locus_tag="OO339_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5927963.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5380..6072)
                     /gene="torR"
                     /locus_tag="OO339_003466"
     CDS             complement(5380..6072)
                     /gene="torR"
                     /locus_tag="OO339_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5927964.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6159..7331
                     /gene="torC"
                     /locus_tag="OO339_003467"
     CDS             6159..7331
                     /gene="torC"
                     /locus_tag="OO339_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5927965.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7331..9871
                     /gene="torA"
                     /locus_tag="OO339_003468"
                     /pseudo
     CDS             7331..9871
                     /gene="torA"
                     /locus_tag="OO339_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9868..>10055
                     /gene="torD"
                     /locus_tag="OO339_003469"
     CDS             9868..>10055
                     /gene="torD"
                     /locus_tag="OO339_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5927966.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2416 a         2590 c         2696 g         2353 t
ORIGIN      
        1 ctccctcgtg gagccttttt tatatctgcc ttatttttct tcaacgctgt atgtatagta
       61 agcgataacc tgttgattat tgaatctttc gggaagatgg cttataacat ttcttacctg
      121 accagggtac cgggaaccaa caccttactg gcgtgttgct gtctttcaag accagacgag
      181 gttaacagtg aatattgaag agttaaaaaa acaagccgaa acggaaatcg ccgactttat
      241 cgcgcaaaaa atcgccgagc tgaacaagaa tacagggaaa gaagtctctg aaattcgctt
      301 catcgcacga gaaaaaatga ccgggcttga aagttatgat gtcaaaatca aaataatgtg
      361 attttgtgaa catcaccccg tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa
      421 tcattggcgc ttgtggaatt aagcgtcggt acaattcctc cggcaccggg ctttgccata
      481 ctcccgcata cattgcgtaa ccaatcaccg caaacataat ccccagaacc agtagcgtca
      541 ttaaccagcc agacaacgca aaggcttttt tatttgctgc aggtttttgc agtgaaaagg
      601 tcaatgttga ggctaccgga catgactcta cgcaagtcat acagccggta cattccactg
      661 ttcgtacctg aattaattta tctaccggga tccgtgatgg gcactttttt gcgcatttgc
      721 cacaatcgat acaactttcg gcattgcgac gaatcttaaa cggcgacaat agcgaaacca
      781 cgcccatcag cgcgccatat gggcaaagat aacgacacca ggcatggcga ataaacaggc
      841 tggcaatcag caaaacggtc acgctgatta atgtcgcggt ccccatatga cgaaagaaat
      901 cgagcatttt aacgtccatc accacgctat agggcgacaa cataaaatag tgaatcgcct
      961 gagcgggcat caataacgcg atatagagaa ataaactcaa cagcaaatac ttcacgccgc
     1021 gcagaggaat atccagccag cggggaagga cacattgccg accaaacagt ttgttaccga
     1081 gatcgccgat taattcagaa agcgtaccaa ccgggcataa ccatgagcaa aaggcctttt
     1141 tgagtaatag actgatgacg ataaaagcga ccaataacag catcgcggcg gcgtggacgg
     1201 acggtaactg acctgttgca aggctatatt tcagattcat cagaccggca atcggtagcc
     1261 agccttcgat acctcccggt ctggcgacaa atgtcgtact acctgccgtt tcgtaatagc
     1321 gcacccaata ccagaacgtg atggcaatat aaatattcat tgccaatagt aagaattgcg
     1381 tcgctttacg ccaggtcgtg gcattgcgcc agtcattcca cggtaatttg ccgcccgtcg
     1441 tgcctggccg ccgctgccag cgggttcttt ttttctctgc catgattttg ccagtccgtt
     1501 aagttgcata ccaaatgcca ctattctagt tattcttaac tggctgatat tgattcaaat
     1561 cgcgttcagg tctttcttat tcaatcatgc ttccagagcg gcaacactgc gtgtaatttc
     1621 ttcgtgtgga aggggggcag ataatggctg ctgctccagt tgtgcgcata gctgactggc
     1681 gctacgcatt cccagactcg agcaactgct ttttagctga tgcgcggcac gctttatttt
     1741 ctcgctgtcc tggctggcgc gggcaatgtc gatttcatcg agaagcggca gggcatgttg
     1801 tttaaataat gccagccatt cgtggatctt ctccgtcccc attaactgag cgtcttcatt
     1861 gagttgcgag acatccagtg gtagatcgtt attggcttgc agttggagat agtgcgccag
     1921 taactgaccg agcacttcac gcggcaccgg tttagggata atcccgcgaa atagtgaact
     1981 ggtacgctgg cgcagcgttt cgtcaatgac atgggcgcta aagccaatca aaaccagcga
     2041 cggatattgc tgtgccagtt gccgggcaag ggtaatgccg tcgatatccg gcagatcaaa
     2101 atccaccagt gcggcagcaa acggttcgct attttgcagt gtctctaaag cctgcgcggc
     2161 attgccaata gcaacaacct gcgcaccact ggtgttcagc atctcaacgg taattcgctg
     2221 ggttagcggg ttatcttcaa ttaacagcaa acgtaaaccg tcaagacgca ccgcctgatt
     2281 ggctgttttt ggcacgggtg cagtggcaac acgtaacggc aagcgtaaac aaaaacagct
     2341 tccaacctcc ggcgtgctgg tggcgctcag ttcgccgccc atcgcctggg ccagacggct
     2401 actgatagtc agccccagcc cggtgccgcc gcgtttgccg cttacctgca caaatggctg
     2461 gaagatttct gccagtttcg cgggatcaat accgcagccg ctgtcttcca cttcgaccag
     2521 ccattgctcg ccatcagtgc gactacgcag gataatgtgc ccttcgtcag taaaacgcag
     2581 ggcattgctc aataggttgg taatgacctg acgaatacgt cgtggatcgc ccattaacgc
     2641 gctcggtaca tcatcggcaa ttgccgttgc caggcgaatc gggcgacctt tcacccgtcc
     2701 gctcattaat tgcagggtac tttccagcag cgggcgcggt tcaaagggct catcgctgac
     2761 tgaaacattt ttgccacctg cttcgatagc cgaataatcg agaatatcgt tgaggatggt
     2821 cagcaacgat tcgccagagt cggtaatcgc ctgcaaatca tcacgctggg cgttaagtgc
     2881 ggggttatct gccagcagtt gagcagtgcc gagaataccg tacagcggtg tgcggatctc
     2941 atggctcatc gccgtcagaa acgccgattt tgcctggctg gctttttctg cttccgcccg
     3001 tgcctgtcgg tgttctatca ccagttcctg caattcagct gtacgcgctt tgacctgcgc
     3061 cgccagctgt tcgcggtggc gattcagtgc atgaacactg ctgcgaaacg catccatcag
     3121 ccgcccgatg gtatccagct cccgtacgcc agcggtttcc gggaaagggg agtcaatatc
     3181 accgtccagc agccgttgca gcgcctgcgt ttgttcggca agtggacgcg tgaatgagcg
     3241 ataaaccacg cgccagagga tcataatcag tgcgcaaagt gaaaccatcc ccagcaataa
     3301 caggctgtat tgcccgcgtg cactggcttt ttccagatgt gccagtccat gctgattgtg
     3361 cagctcaatg gtgtcgacca gctgactgac ttcgctacta aactgcgcga actgggcgat
     3421 attattttgg gcgagagttt gcaggtgatt gctgatttca ctgtcctgct ggaacagcgc
     3481 caacagatcg ctatattggc taacggtagt taacgttgtt gcgacctgcg cacgaactcc
     3541 cggatcttca atgcgtattt gccgacgttg cagaattttc accgcattat tgagctgctt
     3601 ttccagcgtt ggtgcatttt tctggatctg ctccagcccc agattcatca ccatttgctg
     3661 cacccgcaga gcgctaaggc gcagttcatt catctggtta acatactcaa gatcgatatc
     3721 aatcagccga tcgagtgcac tttcagcagc ctgacgctga tcttgttcga tcaaatcgta
     3781 aatcccggcc tgggtcgctc cagcggaagt tgtcgcatta ttcgcctgac cttgctccag
     3841 gcgtgcgatc tcatcggcgg cagctatctg ctgactgagt tgccgttgtt gctggcgtag
     3901 ttgcagacgc cgccccacca gttccccttg ctgacgtaat gaacgggaga tctcctgctc
     3961 ctgttgttca atagcggtgg tatcaaaacc ttgttcccgt aacgcttgca gcaacgcatt
     4021 aatcttcagg ctttatgcgg tgagcattcg cccctgcgcc tgccacatct tttcgttatc
     4081 ggcactggtc aggttctgcg cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg
     4141 cgccatattc atggtaggaa tcaatgcctg agtattgtct ttttccacct ggctgataaa
     4201 gcgcaggttg taccatccca ccagggtact ggtcagggtt aacagcgcca tcagggcaaa
     4261 gcccatccag agtcttcggg tcagggttaa attcacggtc ggtgcactta aggtgaaaaa
     4321 ggttgagtcg caaagtggaa tgcatctagc ataaagcctt attattgatg aggctatcat
     4381 gcgcgtactg ctatttttac ttctttccct tttcatgttg tcggcatttt cggctgataa
     4441 cctgttgcgc tggcatgatg cgcagcattt cacggtgcaa gcctctatgc cgcttaaagc
     4501 caaacgcgta tggaaactgt gcgcgcttta tcccagcctg aaagattcat actggttatc
     4561 gttgaactat ggtatgcagg aggctgctcg ccgctacggt gtggatttaa aagtgctgga
     4621 ggcaggcggc tacagccagt tggctaccca gcaagcacaa atcgaccagt gtaaacagtg
     4681 gggcgcagag gccattttgc tcggtagtag cacgacgtca tttcccgacc tgcaaaagca
     4741 ggtagcaaat ctgccggtga tcgaactggt aaatgctctt gatgatcccc aggtgaaaag
     4801 ccgcgttggt gtaccctggt ttcagatggg ctatcaacct gggcgatatt tggtgcaatg
     4861 ggcgcacggt aaaccactga acgtgctgct gatgcccgga cccgataacg ccgggggcag
     4921 taaggagatg gtcgagggtt ttcgcgcagc cattgccgga agcccggtgc gtattgttga
     4981 tattgcgctt ggtgataacg atattgaaat ccagcgcaac ctgttgcagg agatgctgga
     5041 acgccaccca gaaatcgacg tcgttgccgg aacggccatt gcggcagagg cggcaatggg
     5101 tgaagggcgt aacctgaaaa cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt
     5161 gtatcgcggg ctgaagcggg gaagagtgat tatggctgcc agcgatcaaa tggtctggca
     5221 gggggaactg gcagttgagc aggccatcag gcaattacag gggcaatcgg tgtctgataa
     5281 tgtcagccca ccgattttag ttctgacgcc gaaaaatgcc gaccgagaac atatccgccg
     5341 ctcgctgtca ccggggggat ttcgtccggt ctatttttat cagcacacat cagcggctaa
     5401 gaaataacct tcaccatgtt gcgtcaccag taaatccgcg ctgagtttat gacgtaaacg
     5461 acgaattaac acatcgacgg tgcgcaggtc agggttttcc acccgacgcg cagaaagcat
     5521 acgcagcaga cgctcacggc tgagaatttc gcccggatta gtcacaaatg ccaccaacat
     5581 ttcatactct gcgcgggtca gtttaatcgg ctcgccatcc cgctccagcg tatggcgcga
     5641 cacattcagg caataacccg caaagcgata gcagtagtcc tgagtgtgcg gttgagcttg
     5701 tcgcgcgagg tcgattcgcc agagcagatt tttcacccgt actaccagtt cgcgcagttc
     5761 cagcggtttg gtgacgtaat cgtctgcgcc catttccagc ccaacaatac ggtcaatccg
     5821 atcgctgcgt ccggtaacca gaataatccc caccgttgag cgttctcgca gggcgcgggt
     5881 taacatcagg ccattttcat cgggtaagtt gatatccagc agaattaaat ctaccggctg
     5941 attctgcata atttcccgta gcccggcacc gctcgccgta acggaaacgg tatacccctc
     6001 ctgagtgaag taggattgta atcgcgcctg ggtaaccggc ttatcttcaa caataacaat
     6061 gtgatgtggc atcagagggt tttactcatt ctgttcatat ctgttcatat ttgctcatta
     6121 agatcgcttc actaaaccat aattctacag gggttattat gcggaaactc tggaacgcgc
     6181 tacgccgacc cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg
     6241 gcattgcgct gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt
     6301 gtgtcagttg ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc
     6361 agaacgcctc cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag
     6421 gcatggtgaa gcgcaaactg gaagcgagca acgacattta ccagaccttt attgcccact
     6481 ccattgatac acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg
     6541 cgcgaatgaa agaaaacaac tcggcaacct gtcgctcctg ccataactac gatgcgatgg
     6601 atcatgccag gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc
     6661 aatcctgcat cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct
     6721 tccgtaagca gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact
     6781 ctattgatat taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc
     6841 tgcctgcttc ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta
     6901 ccggctggac ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca
     6961 tctttgttgc ctcgattcgt ggtgatgtgc agcagcaggt gaaaacgctg gagaaaacca
     7021 ccgttgccga caccaatacc gagtggagca agttgcaggc caccgcgtgg atgaagaaag
     7081 gcgacatggt gaacgatatc aaaccgatct gggcctatgc ggattcgttg tacaacggca
     7141 cctgtaacga gtgccacggc gcaccggaaa tcgcccactt tgacgctaac ggttggatcg
     7201 gcacgctcaa cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcactttgt
     7261 tgaaatatct gcaaatgaat gcgtctgaca ccgcaggtaa ggctcacggc gataagaagg
     7321 aagaaaaata atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca
     7381 actcggcggc ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc cgcgccgtgc
     7441 aactgcggcg caagcggcga ctgaggctgt catctcgaaa gagggcattc ttaccgggtc
     7501 gcactggggg gctatccgcg cgatggtgaa ggatggtcgc tttgtggcgg caaaaccgtt
     7561 cgaactggat aaatatccgt cgaaaatgat cgccggattg ccggatcacg tacacaacgc
     7621 ggcacgtatt cgttatccga tggtacgcgt ggactggctg cgtaagcgcc atctgagcga
     7681 cacctcccag cgcggtgata accgttttgt gcgcgtgagc tgggatgaag ccctcgacat
     7741 gttctatgaa gaactggaac gcgtacagaa aactcacggg ccgagtgcct tgctgaccgc
     7801 cagtggttgg caatcgacgg ggatgttcca taacgcttcg gggatgctgg cgaaagctat
     7861 tgccttgcat ggtaatagcg ttggtacggg cggagattac tctaccggtg ctgcgcaggt
     7921 gatcctgccg cgcgtagtcg gttcaatgga agtgtatgaa cagcaaacct cctggccgct
     7981 ggtattgcag aacagcaaaa ccattgtgct gtggggctcc gatttgctga aaaaccagca
     8041 agcgaactgg tggtgcccgg atcacgatgt ttatgaatat tacgagcagc taaaagcgaa
     8101 agtcgccgcc ggtgaagttg aggtcatcag catcgatccg gttgtcacat ccacccatga
     8161 gtatctgggg cgcgagcatg tgaagcacat tgcggttaac ccgcaaactg acgtgccgct
     8221 gcaactggcg ctggcacata cgctgtacag tgaaaacctg tacgacaaaa acttcctcgc
     8281 taactactgt gtgggttttg agcagttcct gccgtatctg ctgggtgaga aagacggtca
     8341 gccgaaagat gccgcatggg ctgaaaaact gaccggcatt gatgccgaaa ccattcgtgg
     8401 gctggcgcgg cagatggcgg cgaacagaac gcaaattatt gctggctggt gcgtgcagcg
     8461 tatgcagcac ggtgaacagt gggcgtggat gattgtggtt ctggcggcga tgctggggca
     8521 aattggcctg ccaggtggtg gttttggctg gcactacaac ggcgcaggca cgccggggcg
     8581 taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa
     8641 cagtgactac aaaggctaca gcagcactat tccgattgcc cgttttatcg atgcgatcct
     8701 cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc cgctgaaaat
     8761 gtgtattttt gccggaacta acccattcca tcgccatcag cagatcaacc gcattattga
     8821 aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct caacctgccg
     8881 ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta
     8941 cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc cgtagttcga
     9001 ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc gtgaagaagc
     9061 ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca
     9121 acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga
     9181 gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc
     9241 ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc
     9301 cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc
     9361 ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt
     9421 ccgacttcac tcgcagctat gtgagtcgga aacgctgcgt cagcaatata cggtagcggg
     9481 taaagagcca gtgttcatta acccgcagga tgccagcgcg cgcggtattc gtaacggtga
     9541 tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggagcagtag tttctgaccg
     9601 ctatgcaccc ggcgtggcgc gaattcacga aggggcatgg tacgatccag ataaaggcgg
     9661 cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ctgaccatcg acatcgggac
     9721 ttcgcagctg gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa
     9781 tggaacagtg gagcaggtga cggcgtttaa cggccccgtg gagatggtgg cgcagtgcga
     9841 atatgttccc gcgtcgcagg tgaaatcatg accacgctga cagcacaaca gattgcctgt
     9901 gtttacgcct ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa
     9961 atcgccagtg cgcagatggc tgaatggttt tcgttgctga aaagcgaacc gccgctcact
    10021 gcggcggtga atgatctgga aaacagtatt gccac
//
LOCUS       DAJTRH010000124         9881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTRH010000124 DAJTRH010000000
VERSION     DAJTRH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..1015)
                     /gene="bglB"
                     /locus_tag="OO339_003470"
     CDS             complement(<1..1015)
                     /gene="bglB"
                     /locus_tag="OO339_003470"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5927967.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQID"
     gene            complement(1034..2911)
                     /gene="bglF"
                     /locus_tag="OO339_003471"
     CDS             complement(1034..2911)
                     /gene="bglF"
                     /locus_tag="OO339_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5927968.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3045..3866)
                     /gene="bglG"
                     /locus_tag="OO339_003472"
     CDS             complement(3045..3866)
                     /gene="bglG"
                     /locus_tag="OO339_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5927969.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4153..4878)
                     /gene="phoU"
                     /locus_tag="OO339_003473"
     CDS             complement(4153..4878)
                     /gene="phoU"
                     /locus_tag="OO339_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5927970.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4893..5666)
                     /gene="pstB"
                     /locus_tag="OO339_003474"
     CDS             complement(4893..5666)
                     /gene="pstB"
                     /locus_tag="OO339_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5927971.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5758..6648)
                     /gene="pstA"
                     /locus_tag="OO339_003475"
     CDS             complement(5758..6648)
                     /gene="pstA"
                     /locus_tag="OO339_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5927972.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6648..7607)
                     /gene="pstC"
                     /locus_tag="OO339_003476"
     CDS             complement(6648..7607)
                     /gene="pstC"
                     /locus_tag="OO339_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5927973.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7694..8734)
                     /gene="pstS"
                     /locus_tag="OO339_003477"
     CDS             complement(7694..8734)
                     /gene="pstS"
                     /locus_tag="OO339_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5927974.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8982..>9881)
                     /locus_tag="OO339_003478"
     CDS             complement(8982..>9881)
                     /locus_tag="OO339_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5927975.1"
                     /translation="NTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
BASE COUNT         2548 a         2571 c         2286 g         2476 t
ORIGIN      
        1 gatcaatttg ccacccccac tctgaacttt tcagatgcgg attggggatc atattcagta
       61 tgttgccctg cgcattttta ttaatgcttt cgtcgtggga aacacaacca gtcatgtaat
      121 aactaaaaga gatgaaatct acagtatgtt ttaaatcttc tgcgtcactt tcagtcatct
      181 caatggtgat attgtggtcg cggaagaaac gctgcatata gccgggatat tggccacgcg
      241 cctgaacatc accaaagaac atccagcgcc ggttctcttc catggcctgc aacatatcct
      301 gtggctggca ggtgaggggg taaaccagcc caccgagaag catattgccg attttcgctt
      361 cggggatcag gctatgatag gctttaactg cccgcgcact ggcaaccagt tgatgatgga
      421 tcgcctgata aacttccgcc tcgccactct cttctgccag ccccacgccc gtgaatggcg
      481 cgtgtaacga catattgatt tcattaaacg tcagccataa cgctacttta tgttggtagc
      541 gggtaaagac cgtgcgggcg taatgctcga agtgatcgat gaccgcccga ttagcccaac
      601 cgccgtagtt tttcaccagc ccatatagca tttcgtaatg ggataacgtt accagcggct
      661 tgatccccgc ctgcgccatt tcatcaaaca gccgatcgta aaacgctaac cccgcttcat
      721 tcggttcggc ttcgtcgccc tgagggaaaa ttcgcgccca ggcaatggaa atacgcagac
      781 aggtgaagcc catctcggca aataacgcga tatcttccgg gtaacggtaa taaaaatcga
      841 tggcgacatc tttgatattc tctttcccca ggatgcgcgg ttccattttt cccattacgc
      901 catgaggctg taaatctgag gtcgtgatcc ctttgccatc ttccagccag gcaccttcca
      961 cctgattggc agctgttgcg ccaccccaaa gaaatgtttc tggaaatgct ttcataatta
     1021 actcctttta tcgttagcga atgatggata acagcggttc acctgcgctt atctgcgccg
     1081 tgccgtgggg taatacgtct gtaaaatcat cgctattact gattaatacc ggcgtcgtca
     1141 gatcaaatcc ggcctcgcga atagcaggga tatcaaaaga aatcagccga tcgcctgtat
     1201 tgaccttgtc acccacgttg acgtgagcgg aaaagaattt gccgtccagt tttacggtgt
     1261 cgataccgac atgaatcagg atctccacac catcatctga ctcaatgcca atggcgtgta
     1321 atgtggcgaa caacgaagca attcgacccg caaccggaga acgcacttca ccaaccgagg
     1381 gcagaatggc gataccttta cccaacaggc cactggcaaa cgtggtatca gcgacgtgaa
     1441 tgagcggcac aatctctccc gtcatcggtg aacagatacc gccctgctca ggtggtgtaa
     1501 taacctctgg tgttttctct tgcggggcac cctgcgctgg ctgacgttta ctggtgatga
     1561 aatgaagcat caacgtacca acaaatgcgc aacctatggc aatgacaccg ccaataacgc
     1621 tggcccagac ggtgaaatca atccccgttg acgggatggt ctgcatgaag gtgaaaatac
     1681 ttggcaaacc aaaggagtaa actttcgttt gcgcgtagcc aatgatggtg gcccccaatg
     1741 ctccactgat acaggcgata acaaaggggt acttacgcgg caggttgacg ccatataccg
     1801 ctggttcggt gataccaaac agacccgtca acgccgctga tcccgccacc acttttttct
     1861 gcgcatcgcg ttcgcagagg aagacgccga gcgccgcccc gacctgcgcc ataatggcgg
     1921 gcattaacag cgggatcatg gtgtcgtagc ccagcacggt gaagttattg atacacagcg
     1981 gcaccaggcc cctgtgcagt ccgaacatga cgaagatttg ccagaagccg cccattaccg
     2041 cgcccgcaaa tgcaggaacc gcctgataaa gccagagata accggcggca atcatttcgc
     2101 ttatccaggt tgatagctgc cccaccagca gaaaggtgac gggtgtgata accatcagac
     2161 atagcaatgg tgtgaagaaa tttttgattg ccgacggtaa ccacgcatta agtcggcgtt
     2221 ccagaatgct gcacaaccag gcagaaaaaa taatgggaat aaccgatgac gagtaattca
     2281 acaatgtgac cggaataccc aggaaatcca gccccggcgc atccgctttt tgcccgttct
     2341 cgaaggcagt cagaattaat ggatgcacta acgctccacc aatcaccatg gcagtaaatg
     2401 gattaccgcc gaagcgtttg cccgcggtgt atcccaggat tatcgggaag aacccaaaca
     2461 aggcatcact ggcgctgaat aaaattaaat aagtaccact ttgttcggtc gtccactgaa
     2521 aagtgagcgc cagagccagc atacctttca ggatcccggt tgccgccatc agaccgatca
     2581 gaggcgtaaa aatacctgaa ataacataaa caaagcggtt tagcagatta cctttctcat
     2641 cattttccgg tgcctgttgc gctttttcgc caaggcctgc cacgctgtta accgccagga
     2701 agacatcggc cacatggtta cctatgacca cctgaaactg accaccgctt tccaccacca
     2761 taataatacc gggggtcttt ttcagtacct cagcttgcgc tttgctttca tcctttaact
     2821 taaaacgtaa tcgcgttgcg caatgcatca gactcacaat gttatctgcg cccccgactc
     2881 ctgcgactat ttttctggct aactccgtca taacttgccc tctaccgctt tgcggcaaaa
     2941 ctccaaaaaa aaacctgaaa aaaacggcct gacgtgaatc aagcaatttt tttcaggttt
     3001 tgcccgctta gtgcggtaac aatcctttac tcagtaataa tatttcagtg ttctttgcgc
     3061 acgcgctcta tatttatggc taaaaacata atctctgcgg gtgaaatttt acgttgatac
     3121 tgcaaaccaa taaaaatggc gatccgttcc gcacattgcc atgcttgcgg gtaattttgt
     3181 tttactgctt gttgtaatga ttcatcacta tcgttaattg aagcatgttc aagaatacgc
     3241 caggataaaa acttcagatg tgtaaccagt cgctgataac tcaagctttc ttcctggtaa
     3301 ttaaggctga actgaaattt tattaattgc agcatttcgc gcattaactg cgtgacacct
     3361 gcaacatcct ccatatttcc gctcatttgg gcactgacca gatgcatggc aataaagccc
     3421 acttcatctt tcggtaactg cacgcccaac cgtttatcaa taatggttaa cgcttcttcc
     3481 cctagctgga actctttcgg gtaaagccgc tggatatccc acagcaacac gttttgctga
     3541 aagcgtttaa tcgcaaactg gcaatggtca gttagcgaga tataaatact gtcctgtaat
     3601 tttcccaagc gctcctgcgc taaagagata agacgatcac aggttgccat cacctcaaga
     3661 ggaatatgac ttaagagttc gcttaatcgc ccgttcagtt catgactgct caaggcatac
     3721 tctttttcta ttccacttga gttaattctt tcgccagcgc gtttttgaaa gccaattccg
     3781 cgccccatga cgactttttc ccgctgttga tcatcaataa ccaccacaac attattgttg
     3841 agaattttgg tgatttgcat gttcatagca aggacctttt ttataaacaa aaaaaaaccg
     3901 acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta acaatccagt
     3961 catttattaa tggtttttat aacgaacatc caggttcgga aattaattta attgcgtgca
     4021 gagaaaatag caatgcgcta ttgataaaaa tatgaccatg ctcgcagtta ttaactttgt
     4081 gtaattttag gaatttataa agttatatat aacaaatccc aataattaag ttattgggat
     4141 ttgtctggtg aattatttgt cgctatcttt tcccgccagc agtttatcca gctcatcacc
     4201 gcctacatga cggaaatcct gacccttcac gtagtagaag ataaactcac aaatattctg
     4261 gcagcggtcg ccgatacgtt cgattgaacg cgcacagaac agcgcggtaa gtacgctcgg
     4321 aatagtgcgc gaatcttcca tcatgtaggt catcagttga cgaacaatac cttcgtattc
     4381 ctgatcgact tttttatctt cacgataaat acgtaccgct tcgtcaatgt ccatccgcgc
     4441 gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga cggcccagcg actccagact
     4501 taccagcaac ggctgatgct gctgggagaa tttctccagc gcagtacggc agattttgtc
     4561 cgccacgtcg ccaatacgct ccagctcggc aatggtttta ctgatcacca taaccagtcg
     4621 caggtcgctc gccgtcggct gacgtttggc gataatgcgc acgcaggctt catcgatcgc
     4681 cacttccatc atgttgacgt tcttgtcgcc ttcgatcacg cgcttcgcca gatcgctgtc
     4741 ctggttatgc atcgcggtga ttgcatcaga aagctgctgc tccaccatgc cgcccatggt
     4801 catcacctgc gtgcggatac tttccagttc ggcgttgaac tggccggaaa tatgtttatt
     4861 aagattgaga ctgtccataa cgcactcctg aatcaaccgt aacgaccggt gatgtagtct
     4921 tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa ctcaatcaat
     4981 tcgcccaggt acataaatgc cgtgtggtcg gaacaacgcg cagcctgctg catgttgtgg
     5041 gtgacgatca ccacggtgta atcctgcttc agttcggtga tcagctcttc aatacgcccg
     5101 gtagagatgg ggtcgagcgc cgaacacggt tcgtcgagca gcagcacttc cgggcgaatg
     5161 gcgataccac gcgcaataca cagacgctgt tgctgaccac cagagagaga gtaaccgctc
     5221 tggtgcaatt tatctttggt ttcgttccac aatgcggctt tggtcaatgc ccactgcacg
     5281 cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac gaacgccaaa ggcgatgttg
     5341 tcgtagatgg acatcgggaa cggcgtcggt ttctggaaca ccatgcccac tttcgcacgc
     5401 agcagggcga tatcctgaga gttggtcagg atgttgtcgc catcaagcag aatttcacct
     5461 tccgcacgct gctccgggta cagttcaaac attttgttga aggtacgcag cagcgtcgat
     5521 ttaccgcagc cggacggccc gataaacgcc gtaacctggt ttttggcgat atccaggttg
     5581 atgtttttca gggcatggaa tttgccgtag tagaagttca aattacgaac ctgaatttta
     5641 ctcggggcag tttcaaccat actcattgca atcttttcct catcgtacct gatgcttttc
     5701 gctaatcagg cttaccgtag ggccgggtaa ggcgttcacg ccgcatccgg aaaagtgtta
     5761 accgtgttta ttcttcgcaa aaacaacgcg cgccagaatg ttcagcagca ggacgcacag
     5821 ggtaatgatc aataccccgg cccaggccag ttgttgccat tccgcaaacg ggctcatcgc
     5881 aaacttaaag atcgtcaccg gcaggttggc gatcggctgc atcatgtccg tgctccagaa
     5941 ctggttggag agcgcggtaa acagcagcgg cgcggtttca ccggcaatac gggcaatcgc
     6001 cagcaggata ccggtcataa tcccggacac cgacgctttc agcgtaatcg cggagatcat
     6061 cttccacttc ggtgtaccca gcgcataagc cgcttcacgc aggctgtccg gcaccagttt
     6121 cagcatgttc tcagtggtgc ggataacgat cggcacctgc aacagcgcca gcgcaattac
     6181 gcccgcccag ccggagaagt gctccatctg tgccaccacg atggtgtaga caaacagacc
     6241 aaccacaatc gacggcgcgg agagcagaat gtcgttaatg aagcgaatca cttctgccag
     6301 ccaggattta cgaccatatt ccgccagata aatccccgcc ataatgccca gcggcgtacc
     6361 gaataccgtg gcccacaaaa ttaacagccc gctacccgcc agggcgttcg ccagaccacc
     6421 accttccgta ttgggcggcg gcgtcatttc agtgaacagc gccagcgaca taccgtcgat
     6481 accgcgagtg atggtggaca ttaaaatcca gatcagccag aacaggccga aggccatcgt
     6541 cgccatcgag agcgtcagcg caatacggtt tttgaggcgg cgacgcgcct gcattttgcg
     6601 gcgagattca gccagcgccg cagtggtttg catttcaacc atagccatta gcgtgccccc
     6661 tcattcttag ccaggcgcat aatcataaac ttcgatgcgg cgaggacgat gaaggtaatc
     6721 acaaacagga tcaggcccag ttccatcagt gcggcaacgt gcagaccgga ttccgcttcc
     6781 gcaaattcgt tcgccagcgc agaggtgata ctattgcccg gcatgtacag cgaggcgctg
     6841 tcgagctggt aggtgttacc gatgataaag gtcaccgcca tggtttcacc gagcgcgcgg
     6901 cccagcccca gcatgatgcc gccgataaca ccatttttgg tgaacggaag aacgatacgc
     6961 cagataactt cccaggtggt gcagccaata ccgtaggccg actctttcat catcaccggg
     7021 gtttgttcga acacatcacg cattaccgcc gcaatgtacg gaataatcat gatggcgagg
     7081 atcacgcctg ccgcgaggat accgatacca aatgcggggc cagagaacag cgcgccaaca
     7141 atcgggatat tcgacatgat attgccgacc ggctcctgaa agtaaacggc gaacagcggc
     7201 gcaaagataa acaggcccca catgccgtaa acgatacttg gaatggctgc cagcagctca
     7261 atggcgatac ccagcgggcg tttcagccag ccaggcgcaa gctcagtcag gaacagggcg
     7321 ataccgaaac tcaccgggac ggcgatcagc agcgcgataa acgaagtcac caacgtaccg
     7381 tagatcggca ccagcgcccc gtagatatcg ttcggtgcat cccactcttt ggtccatagg
     7441 aaagccagac caaatttctg aatgctcggc caggaggaga tgatcagaga gacaataatg
     7501 ccacccaaca tcaatagcac aatcagcgcc gccagtttta ccagcacgct gaaaattatg
     7561 tcgccctttt tacccggtgg gttaaaagcg ggcttggttg cagccataag ttactcttca
     7621 gttaaacgcg tttacaaagt cattttgtaa tgccggatgc ggcgtaaaac accgtacccg
     7681 gcctggagtt ttattagtac agcggcttac cgctactgtc tttaatattg gtcttccacg
     7741 cagcgcgaac ctgttcaact acactatccg gcaggctggc gtaatccagg tcgttcgcct
     7801 gtttagcccc ggttttgtac gcccagtcga agaatttcag cacctctgtg ccttgttctg
     7861 gtttcttctg atctttgtgg atcagaatga acgtggtaga ggtaataggc catgcatctt
     7921 cgcctttctg gttggtcaga tcctgagcga aggttttgct ccagtctgca ccttttgctg
     7981 cattagcgaa gttttcttcg gtcggactaa ccggtttacc atcagcggag atcagtttgg
     8041 tgtacgccag gttgttctgc ttcgcgtaag catattcaac ataaccaatt gcacccggca
     8101 gacgctgaac gaacgcggcg ataccgtcgt tacctttacc gcccagaccg atcggccatt
     8161 ttacggtaga gccagtacca acgttgtttt tccactcttc gttcactttc gccaggtagc
     8221 tggtgaagac gaaggaagtc ccggagccat ctgcgcggcg tactacagca atgttttgtg
     8281 aaggcagttt cagacccgga ttcagtttgg cgatggcttc atcatcccac ttcttgattt
     8341 tgcccaggta gatgtcgccg agggttttac catccagcac cagttcgcca gacttcagcc
     8401 ctggaatgtt aaccgccagc accacgccgc caatcacggt cgggaactgg aacagacctt
     8461 cctgagccag tttttcgtca gacagcggcg cgtcagaggc accaaaatca acggtattag
     8521 cgataatctg ttttacgcca ccggaagaac cgataccctg gtagttaact ttattaccgg
     8581 tttctttctg gtaagtgtca gcccatttgg catacaccgg cgcagggaag gttgcacctg
     8641 cacctgtcag gcttgcttct gcaaacacag agaaagcact catcgataag gtcgcggcga
     8701 caacagttgc gacggtggta cgcataactt tcataatgtc tcctgggagg attcataaag
     8761 cattgtttgt tggctacgag aagcaaaata ggacaaacag gtgacagtta tatataagga
     8821 atatgacatt tttatgacag aaaggaatta ccaaataact tactgatttt aaatgtgaag
     8881 atgaaaattc cacatgccat ctttttgtca tgatgcattt aaaagtgata aagaaatggg
     8941 tgagatgaca acgatgtgat gaacaatcgt tgtcagacct attagcgcac aatctcaaca
     9001 gttatcgtcg ctgttgcagt aaattgcccc ggggctgggc gagctccagt aaggctcgca
     9061 ggagcagctg aaaaagttac actacctgat ttttcaccat acactgttga tttatccatg
     9121 tctgttggca actcaccacc gttcactttg atttccttat tatttttgtc gtaaattttt
     9181 atccccacgt tagagtttga agttacgacc attgaattgt ccgtattggg ttcggtagca
     9241 taaaaagacg tactgatatc atcggtattc agtatcccca ggttttcaca ctcaatagtg
     9301 actgttttag taattttatg tgttgttacc gcacttccaa caacagatga aaaatctgca
     9361 gctggtattt ctttaaaatc aaaacaaact ggctttaaat tatttatttc gcaactttgc
     9421 ggagctgtaa tatcgccccg aatataaaca tcagccatcg gctgtgagta atccaccggg
     9481 gtgtcacggc taattgttcc atacaatgtc actatctttg tttcaggaat aatcaatttt
     9541 cctgcaaatg tcttcttgat ataaaaatat actatcgctt tagccccact ggatatactt
     9601 aaaggttcaa tgcggttgca attgtacttt gtattaccag acgggttgtt agcatgatat
     9661 tcaaaaggtg cattcacata cccaacccca agaatatcta tagcaatccc aacatcaagg
     9721 ttcggattaa gatcataata attaatatta gagtgaaccc cagcataagt taatgcctgg
     9781 tttatttttg acgtataata gatatagggg aattctccta cgttaccagt atcacaatga
     9841 cagcgtccaa gatatgagga tccattagtt acagtggtgt t
//
LOCUS       DAJTRH010000125         9636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTRH010000125 DAJTRH010000000
VERSION     DAJTRH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO339_003479"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO339_003479"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5927976.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="OO339_003480"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="OO339_003480"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5927977.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="OO339_003481"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="OO339_003481"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5927978.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="OO339_003482"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="OO339_003482"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5927979.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="OO339_003483"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="OO339_003483"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5927980.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="OO339_003484"
     CDS             5060..6193
                     /locus_tag="OO339_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5927981.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="OO339_003485"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="OO339_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5927982.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO339_003486"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO339_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5927983.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO339_003487"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO339_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5927984.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO339_003488"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO339_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5927985.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO339_003489"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO339_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5927986.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="OO339_003490"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="OO339_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5927987.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2447 a         2472 c         2458 g         2259 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgcgc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aaccactgag
     9541 aaatttaatc tttgcacgtt gcataacctt taattattca tatgcattgc tgttgttact
     9601 ttttatatag ctaacaccaa taattaacct ttcttt
//
LOCUS       DAJTRH010000126         9518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTRH010000126 DAJTRH010000000
VERSION     DAJTRH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            137..1768
                     /gene="oppA"
                     /locus_tag="OO339_003491"
     CDS             137..1768
                     /gene="oppA"
                     /locus_tag="OO339_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5927988.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1854..2774
                     /gene="oppB"
                     /locus_tag="OO339_003492"
     CDS             1854..2774
                     /gene="oppB"
                     /locus_tag="OO339_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5927989.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2789..3697
                     /gene="oppC"
                     /locus_tag="OO339_003493"
     CDS             2789..3697
                     /gene="oppC"
                     /locus_tag="OO339_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5927990.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3709..4722
                     /gene="oppD"
                     /locus_tag="OO339_003494"
     CDS             3709..4722
                     /gene="oppD"
                     /locus_tag="OO339_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5927991.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4719..5723
                     /gene="oppF"
                     /locus_tag="OO339_003495"
     CDS             4719..5723
                     /gene="oppF"
                     /locus_tag="OO339_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5927992.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5776..6105)
                     /locus_tag="OO339_003496"
     CDS             complement(5776..6105)
                     /locus_tag="OO339_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5927993.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6140..7600)
                     /gene="cls"
                     /locus_tag="OO339_003497"
     CDS             complement(6140..7600)
                     /gene="cls"
                     /locus_tag="OO339_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5927994.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7743..7916
                     /locus_tag="OO339_003498"
     CDS             7743..7916
                     /locus_tag="OO339_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5927995.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(7971..9224)
                     /gene="kch"
                     /locus_tag="OO339_003499"
     CDS             complement(7971..9224)
                     /gene="kch"
                     /locus_tag="OO339_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5927996.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2474 a         2245 c         2373 g         2426 t
ORIGIN      
        1 aataaagtcg gtgatagtaa tacgtaacga taaagtaacc tgacagcaga aagtctccga
       61 gcttgtgcag ggtcccaatc cgggattaca catgctggtt aataccagta attataatga
      121 gggagtccaa aaaacaatga ccaacatcac caagagaagt ttagtagcag ctggcgttct
      181 ggctgcgcta atggcaggga atgtcgcgct ggcagctgat gtacccgcag gcgtcacact
      241 ggcggaaaaa caaacactgg tacgtaacaa tggttcagaa gttcagtcat tagatccgca
      301 caaaattgaa ggtgttccgg agtctaatat cagccgagac ctgtttgaag gcttactggt
      361 cagcgatctt gacggtcatc cagcacctgg cgtcgctgaa tcctgggata ataaagacgc
      421 gaaagtctgg accttccatt tgcgtaaaga tgcgaaatgg tctgatggca cgccagtcac
      481 agcacaagac tttgtgtata gctggcaacg ttctgttgat ccgaacactg cttctccgta
      541 tgccagttat ctgcaatatg ggcatatcgc cggtattgat gaaattcttg aagggaaaaa
      601 accgattacc gatctcggcg tgaaagctat tgatgatcac acattagaag tcaccttaag
      661 tgaacccgtt ccgtacttct ataaattact tgttcaccca tcaacttcac cggtgccaaa
      721 agccgctatc gagaaattcg gcgaaaaatg gacccagcct ggtaatatcg tcaccaacgg
      781 tgcctatacc ttaaaagatt gggtcgtaaa cgaacgaatc gttcttgaac gcagcccgac
      841 ctactggaac aacgcgaaaa ccgttattaa ccaggtaacc tatttgccta ttgcttctga
      901 agttaccgat gtcaaccgct accgtagtgg tgaaatcgac atgacgtata acaacatgcc
      961 gatcgaattg ttccagaagc tgaaaaaaga gatcccggac gaagttcacg ttgatccata
     1021 cctgtgcact tactattacg aaattaacaa ccagaaaccg ccattcaacg atgtgcgtgt
     1081 gcgtaccgca ctgaaactag gtatggaccg cgatatcatt gttaataaag tgaaagcgca
     1141 gggcgacatg cccgcctatg gttatactcc accgtatact gatggcgcaa aattgactca
     1201 gccagaatgg tttggctgga gccaggaaaa acgtaacgaa gaagcgaaaa aactgctggc
     1261 tgaagcgggt tataccgcag acaaaccgtt gaccatcaac ctgcggtata acacctccga
     1321 tctgcataaa aagctggcga ttgctgcctc ttcattgtgg aagaaaaaca ttggtgtaaa
     1381 cgtcaaactg gttaaccagg agtggaaaac gttcctcgac acccgtcacc agggcacttt
     1441 tgatgtggcc cgtgcaggct ggtgtgcaga ctacaacgaa ccaacttcct tcctgaacac
     1501 catgctgtcg aacagctcga tgaataccgc gcattataag agcccggcct ttgacagcat
     1561 tatggcggaa acgctgaaag tgactgacga ggcgcagcgc acagctctgt acactaaagc
     1621 agaacaacag ctggataagg attcggccat tgttcctgtt tattactacg tgaatgcgcg
     1681 tctggtgaaa ccgtgggttg gtggctatac cggcaaagat ccgctggata atacctatac
     1741 ccggaatatg tacattgtga agcactaatg gcaatacgtg gggcaggagt gtcctgctcc
     1801 acggtgtctg atttttatcg cattacagaa ggcacaggcc agaaggtagg gcaatgttaa
     1861 aatttattct acgtcgttgt ctggaagcga ttccgacgct atttattctt attactattt
     1921 cgttctttat gatgcgcctc gcaccgggaa gcccttttac cggcgaacgt actttaccgc
     1981 cagaagtgat ggccaatatc gaagcgaaat atcatcttaa tgatccgatc atgacacagt
     2041 atttcagcta cctgaaacaa ctggcgcatg gcgatttcgg tccatcgttt aaatataaag
     2101 attattcggt caatgacctg gtggcatcca gttttcccgt ttctgccaaa ctgggagccg
     2161 ccgcattttt ccttgcggta atactgggtg ttagtgctgg cgttattgcc gcattaaaac
     2221 aaaacaccaa atgggactat accgtgatgg ggctggcaat gaccggggtt gttatcccca
     2281 gttttgtggt tgcgccatta ttagtcatga tatttgcgat cattttgcat tggctgccgg
     2341 gcggtggctg gaatggtggg gcgcttaaat tcatgatatt gccgatggtg gcgttgtcac
     2401 tcgcctatat cgccagtatt gcgcgtatta cccgtggctc tatgattgaa gtattacact
     2461 ccaactttat tcgtactgcc cgggcgaaag ggttacctat gcggcggatc attttacgcc
     2521 atgcattaaa acctgctctg ttacccgtgc tctcctatat gggccccgca tttgtcggca
     2581 ttattaccgg ttctatggtc atcgaaacca tttatggttt gccggggatt gggcaactgt
     2641 tcgttaatgg cgcattgaac cgtgactatt ccttagtgtt aagcctgacc atcctggttg
     2701 gtgcgttaac cattttgttt aatgccattg tcgatgtgct atatgcggtt atcgacccga
     2761 aaatccgtta ctgatactgg agctcgcgat gatgttaagt aagaaaaaca gcgagacgct
     2821 ggaaaatttc agtgaaaagc tggaggtcga agggcgcagc ttgtggcagg acgcacgtcg
     2881 acgttttatg cataaccgtg cggcggttgc cagtctgata gtgctggtgc tgatcgcgtt
     2941 atttgtaatc ctggcaccga tgctttccca gtttgcctat gacgatactg actgggcgat
     3001 gatgtccagc gccccggata tggagtccgg tcactacttt ggtactgact catccggtcg
     3061 cgacctgctt gtgcgcgttg cgattggcgg gcgtatctca ctcatggtcg gtgttgctgc
     3121 ggcactggtg gcagtggtcg tggggacact ttatggttcg ctttccggtt atctgggcgg
     3181 taaagtggat tcggtaatga tgcgtctgct ggaaatcctc aactccttcc cattcatgtt
     3241 cttcgtcatt ttgctggtga cctttttcgg ccaaaacatc ctgctgattt tcgtggcgat
     3301 tggcatggtt tcctggctgg atatggctcg tattgtgcgt gggcaaaccc tgagtctgaa
     3361 gcgcaaagag tttattgagg cggcacaagt tggcggtgta tcgacgccgg gcattgttat
     3421 tcgccacatt gtgccgaacg tactcggtgt ggtggtggtc tacgcatcgc tactggtgcc
     3481 cagcatgatc ctctttgaat ctttccttag cttcctgggg ttgggtacgc aagagccgtt
     3541 aagcagctgg ggggcattgc tgagtgatgg cgcgaactcg atggaagtct ctccatggtt
     3601 actgttgttc ccagcgggat tccttgtggt gacactgttt tgtttcaact ttatcggcga
     3661 tggcttgcgt gatgccctcg acccgaaaga tcgttaagga gtgcagccat gagcgtaatt
     3721 gaaactgcaa ctgtgccgct cgcacaacaa caggctgacg cactgctgaa cgtgaaagat
     3781 ttgcgtgtca cctttagtac cccggacggc gacgtcacgg cggtcaatga tttaaatttt
     3841 tccctacgtg ccggagaaac gctgggcatt gtaggtgagt ctggttcggg taaatcgcaa
     3901 actgcatttg cgttgatggg cctgttggct gccaacggac gcattggcgg atcggcaacc
     3961 ttcaatgggc gtgaaatcct caatttgcca gagcgtgaac tcaataaact gcgcgctgaa
     4021 caaatctcaa tgatttttca ggacccaatg acttcgttga atccctatat gcgcgtcggt
     4081 gagcagttga tggaagtgct gatgctgcat aagaacatga gcaaagctga ggcgtttgaa
     4141 gagtcggtgc ggatgctcga tgcggtaaaa atgccggaag cacgtaaacg catgaaaatg
     4201 tacccgcacg aattttctgg cggcatgcgt cagcgagtca tgattgcgat ggcattgcta
     4261 tgtcgaccta agttgctgat tgcggatgaa cccactacag cgctggacgt caccgtacag
     4321 gcgcagatca tgacgctatt gaatgaactg aagcgggaat ttaatactgc catcattatg
     4381 atcacccacg atcttggcgt ggtggcgggg atctgtgaca aagtgctggt aatgtacgcc
     4441 gggcgcacga tggaatatgg caatgcgcgc gatgtctttt atcaacccgt tcatccttat
     4501 tctatcggtt tgctcaacgc ggtgccgcgt cttgatgcgg aaggtgaaac aatgttgacc
     4561 atcccgggta atccgccaaa cttgctgcga ttaccgaaag gttgcccgtt ccagccacgt
     4621 tgtccacatg cgatggaaat ttgtagtagc gcaccgccgc tggaagagtt tacgcctggc
     4681 cgtctgcgtg cttgctttaa accggtggag gaactgttat gaatgctgta actgaaggaa
     4741 gaaaagtcct ccttgaaatc gccgatctta aagtgcactt tgaaatcaaa gatggcaaac
     4801 agtggttctg gcaaccgccg aaaacgctca aagccgtcga tggtgtcact cttcgcctgt
     4861 atgaagggga aacattaggt gtggtagggg aatcgggatg cggtaagtcc acctttgctc
     4921 gcgccatcat cggtttggtc aaggcgaccg acggtcatgt tgcctggtta ggtaaagagt
     4981 tgctgggcat gaagcccgat gaatggcgtg ccgttcgcag tgatattcag atgattttcc
     5041 aggatccgtt ggcatcgcta aacccgcgta tgaccatcgg cgagatcatc gctgaaccac
     5101 tgcgtactta tcatccgaaa atgtcacgcc aggaagttcg cgagcgcgtg aaggcgatga
     5161 tgctgaaagt cgggttattg cctaacctga ttaaccgcta tccgcatgag ttctccggtg
     5221 ggcagtgcca gcgtatcggg attgctcgtg ctcttattct tgaaccgaag ctgattatct
     5281 gcgatgagcc ggtttcggcg ctggacgtgt caattcaggc gcaggtggtt aacctgctcc
     5341 agcagctgca acgtgagatg ggattgtcat taatttttat tgctcatgac ctggccgtgg
     5401 taaaacacat ttccgatcgt gtgttggtga tgtatctcgg ccatgcagta gaactgggga
     5461 cctatgatga ggtctaccac aatccactac atccttacac cagggcattg atgtcggcag
     5521 tccccatacc tgatccggat ctggagaaga acaaaaccat ccagttactg gaaggggaat
     5581 taccgtcgcc gatcaacccg ccttccggtt gtgttttccg tacccgttgc ccgattgccg
     5641 gtccggagtg cgccaaaaca cgtcctgttc tggaggggag tttcagacac gccgtttctt
     5701 gcctgaaagt cgatccgctt taaaaacaat aagggctgac agttgtcagc cctttttcac
     5761 gctaaaagcg attatttatt cccgccagat gatatggcaa agtttgtgat ctttttcgcg
     5821 gcataacaga atgcgggcga aaacatcgct gatttcacca tcttcactgt ccgccagacc
     5881 aatcaccact tcggcaaaaa agtccgggtt caaatcgaaa tcaacatgtt cctgccagtc
     5941 ttccgccgga tcgaataact ccgcgccgcc gcgctcttca aactgaagat tgaacagcag
     6001 aacatcagct ggatcgagat tgtcggcagc cagttcgaga aaaatatcgt aggcctgctc
     6061 aagcgtttca tcttcagtca ggcgattgtt tagatccata tccatagtca ctacctgttt
     6121 aacctctgtt ggcgacgttt tacagcaacg ggctgaagaa gtaaaacagt cgctcggcga
     6181 cacgttgcca taatggacgt tttagccata aacgggcatc gagcagacgt gaacgcgaaa
     6241 tataatcgtc ctgaacggcg gcaaggtctg caccaaaacc tttatcgtcg attgccaagg
     6301 taatctcgaa attcagccac agactacgca tatcaaggtt aacggtgcca accagactta
     6361 gttcgccatc gaccagcacg ctcttggtat gcagtaaccc gccttcaaac tgataaattt
     6421 taaccccagc agcaagcagt tccgtaaaga atgcgcgact ggcccagccg accagcatcg
     6481 agtcattttt tcgcggaagg ataatactga catccacccc gcgctgcgcc gccgtgcaaa
     6541 tcgcatgaag taaatcatcg cttggcacaa agtagggcgt ggtcatgatc aaatattcat
     6601 gcgccgaata agccgcagtc aataatgcct ggtgaatgag atcttccgga aagccggggc
     6661 cagaagcaat tgtgtgaatg gtgtgaccgc tggcctgttc aaacggcata atattgacat
     6721 ctggtggtgg cggcagaata cgttttccgg tttcaatctc ccagtcgcag gaataaataa
     6781 tccccatcgc ggtggcgatg gggccttcca tacgcgccat cagatcaatc cattgcccta
     6841 cgcccgcatc ttgtttgaag tagcgaggat cgaccatatt catgctgccg gtgtacgcga
     6901 tgtaattatc gatcatgatc atcttgcgat gttggcgcag gtccatacgg cgtaaaaaca
     6961 cacgcatcag attgaccttt aaggcttcga ccacttcaat accggcatta cgcattagct
     7021 cgggccacgg gctgcggaaa aaagccacac tcccggcgga gtcgagcatc aatcggcaat
     7081 gaataccacg ccgtgcagcc gccattaatg attcagccac ctggtccgcc atgccgccgg
     7141 gctgccagat ataaaacacc atctcaatat tatggcgcgc gagctggatg tcgcggatta
     7201 acgcctgcat cacatcatct gactcggtca tcagctgtag ctgattccct ttgaccccag
     7261 cgatcccctg acgacgctcg caaagcttga ataatggcgc agcgacactg ctattttctt
     7321 cggcgaagat atgcttacag gctttaaggt cgttaagcca ttttgcggtg gaaggccaca
     7381 tcgctctggc gcgctcagcg cggcgtttgc ctaaatggag ctcgccaacg gcaagatagg
     7441 caataattcc gactaacggc agaatgtaaa taatcaacag ccaggccatc gcggagggaa
     7501 ctgcgcgtcg tttcattaga atgcgtaaag ttacgcctgc aatgagcaac cagtatccca
     7561 gaatggccaa ccaactcacc aacgtataaa cggttgtcat agattagaaa tccttttgaa
     7621 agcgcattgt tatgagttta cgcatatgga ttcatctggc aaataaaaac gcgggaaaag
     7681 cactggtcag cgtcgggggc gggggtataa tgaccattct gttattgcat agagtagtta
     7741 acatgaagcg gagtagaacg gaagtggggc gctggcgcat gcagcgtcag gctagccgac
     7801 gtaaatcgcg ttggcttgag gggcaatcgc gccgaaatat gcgtatccac agcatcagga
     7861 agtgcattct aaacaaacag cgtaactcgt tattgtttgc gatctacaat atctaaatgt
     7921 actgggcacc attgcggtgc ccaataagtt tactcttccg tctttaaact ctatttttgc
     7981 gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctgaa aataccatga
     8041 cctgagttca acaacattga aacgagcata tcattattaa tctcttcacc atttaaaact
     8101 cgcgccagaa tttcgctgcc aaacagttgc ggtgagagaa tgatatccgg atgtaccatc
     8161 ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac atcactgctc
     8221 atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact cagcgccaga
     8281 atggcccggc aacgatcgat tcccgctttc tttaatactg aactgtcatt actgtcaccg
     8341 gggataacat cagcgttatc gcctaagcgt tgctcaagtt gcttgatatc atcttcaggc
     8401 aagttgctga taaccgttac gttttgtccg cgttgattca gttgcaggat cgtattgatg
     8461 gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt atgattgttt
     8521 ccttttaaaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga ggtcatagat
     8581 gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg tgctgattca
     8641 gaaacaggga caatatctcc atagccgacg gttgacatgg tttctatcga aaaatagaac
     8701 gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag cgcaccgtag
     8761 gttgagtaaa acagtaatgt cgtgaaacta ataaatgcaa aaattgtccc ggctgcggcg
     8821 ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa aatgcaaaat
     8881 ccaatactaa atttcagcca gggataaaaa tgcagggtgt aaattagcgc tatcaacaac
     8941 aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat ggagttcagg
     9001 acaagaaaga cgccgagcat aaatagtggg gcgtttgcca gcaaggagag atccaactct
     9061 gaaaatgctt tgatatggaa aatatcaagc aaattgaccg acataccata gattgtttta
     9121 aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg tagggtcacc
     9181 cataaatttg ttgctgtttg tttgaatgta gcccagtgac tcactgatac ctctgtaacc
     9241 acaattttgt ttaattatct tagcgtgcac attttctgaa catacatgca gcgcgtgaat
     9301 gtgttaaaca atagtagatg actttatccg cagtaatcaa aggttttgag tgagcaactc
     9361 tgcgtcagga aagggctttt ttcctgaaca tgctcacatc atataaagaa atacagataa
     9421 agttattatc tgcttgtggt agtgaatgca ctgaccggct ataaggaaag gccaaacaag
     9481 aacacggttg caaaaaccgt gcccttaaat attggatc
//
LOCUS       DAJTRH010000127         9483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTRH010000127 DAJTRH010000000
VERSION     DAJTRH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..477)
                     /gene="xdhC"
                     /locus_tag="OO339_003500"
     CDS             complement(<1..477)
                     /gene="xdhC"
                     /locus_tag="OO339_003500"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5927997.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(474..1352)
                     /gene="xdhB"
                     /locus_tag="OO339_003501"
     CDS             complement(474..1352)
                     /gene="xdhB"
                     /locus_tag="OO339_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5927998.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1363..3660)
                     /gene="xdhA"
                     /locus_tag="OO339_003502"
                     /pseudo
     CDS             complement(1363..3660)
                     /gene="xdhA"
                     /locus_tag="OO339_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4075..4830
                     /locus_tag="OO339_003503"
     CDS             4075..4830
                     /locus_tag="OO339_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5927999.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4909..4982
                     /locus_tag="OO339_003504"
     tRNA            4909..4982
                     /locus_tag="OO339_003504"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4941..4943,aa:Gly,seq:ccc)
     gene            5117..6727
                     /locus_tag="OO339_003505"
     CDS             5117..6727
                     /locus_tag="OO339_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5928000.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6759..7366
                     /locus_tag="OO339_003506"
                     /pseudo
     CDS             6759..7366
                     /locus_tag="OO339_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8297..9043
                     /locus_tag="OO339_003507"
     CDS             8297..9043
                     /locus_tag="OO339_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5928001.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9341..>9483
                     /locus_tag="OO339_003508"
     CDS             9341..>9483
                     /locus_tag="OO339_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928002.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPS"
BASE COUNT         2416 a         2349 c         2412 g         2306 t
ORIGIN      
        1 cttcgttttc tcgcaatcca gaactgtatt tacaatcatc tgataccccg tgcagcgaca
       61 aagatttccc gccagtccgc gacgaatttc cgtaatggtt aatggcttct cgcgtggttt
      121 cgccagcatt gccgtggtag ccataatcag gccaggcgta caaaacccgc actgcactgt
      181 gccggatttc gcataagcct gctgaacatg agaaagtttt ccgcctttcg cttcaccttc
      241 cagcgtgcgg atctcttttc cttcagccca ggcggcaagg tataagcaac tgtctattgc
      301 tgtgccgtcg accaacaccg tacaggcacc acattcaccc acgcagcacc cttgtttgac
      361 acttagcagt ccttgttcgc ggagtaattc cgagagcggc gtgcctggtg cggcgtgaag
      421 ctgaaaaggc atcccgttaa tggtgcattc ggtggtaatt gtttcgctgt gattcattgc
      481 aattttcccc ccgccgcggc gacggcttcg ctaatcactt ttttggtcat cgtctggatg
      541 agatgcagac gaaactcttt actggcccgc catgaagaac gcggggcgac atcttgcagg
      601 acagattcgc tgatagcttc cagcgtttgc aggtttaatg gcgcattttg tgcagtctgt
      661 tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac caaatgccag gcgtaattcg
      721 ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc caatcgttga aatatccatt
      781 gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt gttctttcgg ctgtggcgga
      841 aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca ctttgcccgg cccggtgtga
      901 aagccattaa tcgggacgaa acgaacaccg cgcggggagt ggatctccag tttcgcgtca
      961 taaattagcg ttggcgtggc agaatctgcg ctggtggcac cgttgcaaat atttccaccg
     1021 taggtagcga cgttacggat ctgcggtcca gcaatgaacg tggccgcagc acataacgcc
     1081 gggagatgac gttgagttat aggatcttct attagctggg taaatgtcgt tgcagagccg
     1141 atacgtagcg agccatcttc cgccagcgta attccccgca gctccgccag attatgaata
     1201 tcaacaatat ggcgataacg gtcattgtga tggtggagct gaatcagtac gtcagtgcca
     1261 ccggcgagca gtttggcctg cgggttgtca gccagcaggt tgatggcatc ggcaagggtt
     1321 gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat cctcaaatca atcctgccag
     1381 atggaactct tcatataacc gttttggcgt cagcggcagt gtattgattg caacaccggt
     1441 agccatcttc accgcgttac gaatagcagc ggcaacagga attatgggtg gctcacccag
     1501 tgacttatgt ccgtatgcgg attgcggctc attgatttcg acgaacgcgc tttccagttg
     1561 tggcagatcc ggcatggtcg gcattttgta atccagcaga ttggggttac ggaccacgcc
     1621 gcttttagca tcgatgatca tctcttcaaa tagcgcccag ccaatgccca ttcccattcc
     1681 gccgtgtacc tgaccttctg ccagcagtgg attaagaata tgccctgaat catgaacgtt
     1741 gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg accgtcagat caacaaaggt
     1801 acagccaaac gccggtgggt tagtggtggt tttgatggag ctttcagcag agagctgccc
     1861 gccgcgttca gggtggtaga aagcgtccat cgccaaatct tttaacgaca ttaacggctc
     1921 ttccggtcgt tcaaccagca cgatatggcc ttttatcagg gtcaaattca tcgctgactg
     1981 atgtagcatg actgcggcgt gagcgatgat tttctctttt aataatagtg ccgcactgcg
     2041 cagcgcaggc gcggcaacat agctctggcg tgaggcaaat gcgccgggat cgaacggcgt
     2101 aacgtcggta tcttgtgttg aaataacgcg aacgtcgctg accggaaccc ccacggtttc
     2161 tgccaccatt tgcgagaaga cggtgtcggc accctgaccg atttccgtcg cgccgctttg
     2221 cacgttgatg gttccatcct gattcatcag aaggcgcgcg cctgctattt ctacgccgac
     2281 aggccaggtg ttagaggtgt agctaaaaca ggcgacgcca acgccgcggc gcaaattgcc
     2341 ttgctggttc tggcattctg cacggcgttt ttcccattca aagattttcc ggcctttttc
     2401 aagacactcc ggcaaccctg cgctgcaaat acgtttgccc gtgagcggat tagcatctcc
     2461 ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca atacctaacg ctgtcgcggc
     2521 gtcatcaagc atagactcaa cggcaaatac gacttgtggc gcgccataac cacgcatcgc
     2581 accagccgag gggaggttgg tatagcaggt ctttgaactg taagcgtagg cacaacgagg
     2641 ataaaggtaa gcgactttat tcccccccgc agaagcgatg gagtgcccgt gagatgcata
     2701 agcgccggtg ttagacagaa catccagact ataacctttc aaggttccgt cgcggttcac
     2761 gcccatttgc ccgtcaatag taaaagcgtg gcgggtacgg gttgcgagga aacactcttc
     2821 acggctaagg gaaactttca ccggaatgcc gccaagcttg ctggtcagga atgccgccat
     2881 tggctcttcc agtacatcct gtttattacc aaaaccgcca ccgacaaacg gtttgatgac
     2941 tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc acgcggcgaa caatgtgcgg
     3001 gatctgggtg ctggaaacga tggtaattcg cgagtcatcc tccatccacg ccagcgatgt
     3061 tacgctttcc atgtgacaat gttgaataac gggagtctga tagtgcccct gtacctggta
     3121 gtcggcggca tcgattgttt gttggacatt gcccgtcgac atcgtgcttt gtttcagtaa
     3181 attgccgccg ttatggattg gtgcagcgtc ttctgtcagc gccgcttctg gcgtggtgat
     3241 aacgggtaat tcttgccact caatgctgac caattgcgcc gctttttctg ccgtgagttc
     3301 atcgcgggcc acgacgatgg caacggcgtc accatgatga cgaacatggc gagttagcag
     3361 tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc taggcatgcc cagctgtagc
     3421 gaatggaata tcaggcacat cttcccaagt aaaaatcgcc agtacgcccg gtaaacttct
     3481 ggcttgttca tcattaatac ttacggcata accatgtgcg atagggctac gtacatattt
     3541 cgcgtaacac atgcccgcca taacataatc gtcagtatat cgtgcccgcc cggtgacctt
     3601 agcaatggca tcgacgcgca tgcatgattc acccgtagcg gttgcttccc gcgcttccat
     3661 agaaatcccc tcgatttgaa catttggttc ttatggcaaa gatttagtct aagcaggcaa
     3721 gatttacgcc agaaacgacg ataagcgtta ggggaggaag gaagtgtgat gacgttaaaa
     3781 aaacacagat atgatacgaa acaggactct gaatagggac agtgatacgt atcaatatga
     3841 gtggcgataa aatcaatgtg ataaatcagg attattacgc atcaggatgg gaaatagggt
     3901 aaaaagtcag gaacttcgat acctctacag cgaatatttt gctgatgata cttccctttg
     3961 ccaaatgaaa ttaacctctt tctctattac cacgtttttc cagaagcaag agattgggta
     4021 tcaagaggct ggctgctatg atatggcgtc ttgtttttta agcgaggaaa gattttgagt
     4081 gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt ttttatcggt tggactgcta
     4141 ttggcgggct gttcgggtag caaatcatcc gatacaggaa cgtattccgg ctccgtttac
     4201 accgtgaaac ggggggatac gctatatcgt atttcgcgca ccacgggaac cagcgtaaaa
     4261 gagctggcgc gactgaacgg catttccccc ccttacacca ttgaagttgg tcagaaacta
     4321 aaactgggtg gggcgaaaag tagcagtagt acacgtaaat caaccgccaa atcaacgact
     4381 aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat cttcctggcc gccagtaggg
     4441 caacgttgtt ggttatggcc aacgacaggg aaagttatca tgccgtattc gacagcagat
     4501 ggcggcaata aagggattga tatctcagct ccacggggta cacctattta cgccgcgggt
     4561 gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct acggtaatct catcatgatt
     4621 aaacacagtg aagattacat tacggcttac gcccataatg acacgatgct ggtaaataat
     4681 gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg ggagcacgga tgcggcatct
     4741 gttcgcctgc atttccagat tcgttaccgt gcaacggcaa ttgatccgct acgttacttg
     4801 ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta atcagcagtc agcagcgcgg
     4861 cacttgctaa ggagagcgta aggtttataa tgccttacgc atctcgaagc gggcgtagtt
     4921 caatggtaga acgagagctt cccaagctct atacgagggt tcgattccct tcgcccgctc
     4981 catgacactt cttaaagtct actaacttac tgaatcctaa gggatacggc ttaacgcctt
     5041 ccgatagttt tactcactgt aaccaacact gtgactaact ttgttgaagt agtgagcaga
     5101 cctaaggtct cagtacatgc tcaagtcaag aacctacctt tatcagcgta atggtgtctt
     5161 ttacatccgt cttaggatga agaccactag tcgcctgacc gcttcgcttc cttctcacaa
     5221 ccgttataaa ctagcgtcag tatctttacg gaccaaggat agacgtaccg ctatggctca
     5281 ctcacggcac atcaagtcag cacttaaagc aatccatgca gacaacccta acgcctctta
     5341 tgaggagcta cgggagcacc tgaagaccat tgtcgagtgg gaacttagtg tcagccgtga
     5401 tgacctgaac gacccggagt cctaccagct ttacgttgac cagtacgatg acattaagtc
     5461 caaccttcgg gaagctgtag cgaccgagcg tctgactgta gaccagcatc gctatattaa
     5521 tgacgttatc ggtgtgctta aggcctgtca ggatagactc aatggcgata gctctggcct
     5581 attgtcttac cttgagcctg agactggcag tctaagaccg tccgtctccc tatctgtatt
     5641 ggcggaacct gaagtccctg aacctaaagc actgaccctc gcgtctctca ttgagcagta
     5701 cgagcaggag aacgcccaga actggaagcc agcgacccta agcgaaaacc gagcgtcaca
     5761 ctctacgcta atcgagatat tcgactatct ggatattcag gatgtcggca aggcgacccg
     5821 tgcagatatg ctcagagttc gtgaagtcct ccagcagcta ccgaagaacc gcaagcaacg
     5881 ctttaagtct atgccgctgt ctgacttgct gaaccgggag tctaagactg actgtctgga
     5941 cgtcgttacc attaacaata aatatcttat caagatggct gctgtgttta agtgggcggt
     6001 acgcaacgac ttgatagcta agaacctgac cgaaggtctt gagctgaaag tccctcagcg
     6061 taaagcctcc gacgctcgtg atgcgttcag tcctgagcag gtagggcaac tactggtcgc
     6121 agctaaagcg tactcgcaga agacctcagg taagccatac cattattacg tcaccgctct
     6181 ggctgcgatt acaggtgcga gacttaacga ggtggcccaa cttcaggtta aagatgtcag
     6241 gaccactgag gctgggaccg tttttatcca tatcaacgaa gacgatagca gcctgccggg
     6301 taagagtatc aagaacgccc acagtgaccg ctgtgtcccg ttggtagatg gtgcttatgg
     6361 ttttgtattg gctgacttta tgtcactcgt ggaagaccgt agaaagactg agggtgataa
     6421 tgctatggtg ttcaatggcc tcaagctaat gaagaacggc tacggtgagc aggttagcaa
     6481 gtggtttaac cgaacgttgc tgcccaaagt cctcgctgac cgtagcggct tagcttttca
     6541 ctcgtttaga catacagttg cgacccagtt aaaacaacat ggggtcgagt tagcgtatgc
     6601 tcaggcgatt atgggtcata gctcagggtc tataacttac gaccgttacg cgaaagaggt
     6661 cgaggtggaa acgctgaaag aaaaattagc ggagtcattg tcagtaaaaa aaatagacgg
     6721 aaagtaatcg catagggata gcggaatgtg aggacgtaat ggtcaaagaa tttttagtta
     6781 gcaaagaaaa taagccttca tatgaatgta tgaatactgc tgaaacttgt ttggggctgg
     6841 ctggaatggc ttcggaagat tggcaacgtg agcatcattg ccgcacaggt ataatattta
     6901 ctgcttttgc tattgaggca atgttcatct tctatagaaa acaggttgac cccggctacg
     6961 ataagacgca gaaagagtgc agaaagacaa tgcacaagaa tacgttaaaa ttgtgcggta
     7021 taaacaatta tatggggaca aaaccttacc aaattatcaa agagtgtctt gaggtaagag
     7081 acgctattgc tcacggcgat tcgtatactt cgagctttaa tttctcggcg gatcatttgg
     7141 ataaccaaga cgatattgca aaagtgtcct ggcggtaaac tctgaacaat ttcggggatt
     7201 aactgttgag cgccttgcaa aatacattga aatggcaaag catattgacc atgagatttc
     7261 tgataatggt tacaggcctt ctgaaggtta ccttccagca gccgagcgca gaagtctgat
     7321 gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt gcttgagcga taatacacct
     7381 cactatagag gaatggaccc ttaactatca ctatagggga gagaccctaa gtccccacct
     7441 taatagcagt cacgacccat acacacattc attaagtctc aggcttccgg tagcggaccg
     7501 attggaaacc atccgcagac gcctagtgtt aaatcacctt ggcctgagac cattcctttt
     7561 aacgtaagct atcttccctt ttgacaggcg gggagcagta gaccacatcc gactttaagt
     7621 tggctctgtg ataatcgctg cgcggataac tgaagaacca agacaccatt actgaggtct
     7681 gccatccatt tgtggacagg cggaaactca aagaggttag atcagtggtg tctgaggttg
     7741 gagctttgag tcttgaccag caggctggag gagaccatga gtttatcttg ttatggattg
     7801 tgttatcaca attccaataa gaagaacttt aagtccagcc tccactcccg ccaacccgaa
     7861 gtaaaattat ggtcgctatg atgtggtctc catgcttcgg gtcttactgg aagtactgaa
     7921 cactttcgcg tcaagttctc cgaagttcat tcctgtttga ctaaactatc actataggga
     7981 ccatatctcg cctctgacct cctgagagac ggcaagcgag aaaccgtggt ccacgctagc
     8041 aagctagcca ccagcctaag gcaagacagc ctctggtcag cctgtgtgtt ctttggtctc
     8101 gtgacttaag tctatcacca cacacgctcg gacatgctgt cgaaccttca tctctatggt
     8161 catcgcagac cactaacccc aattacctca agtcattctg tttgcgtcct gttgcctgct
     8221 ggtctccctt tacgtggggg cttgagtagg tcagtggggt gcgacttcag ggtgcaaaca
     8281 aaaggagtac acgtaaatgt cccaacaaga aatcagcatc attaacctcg accagcttgt
     8341 gtccatgacc tcagttgaaa ttgctgagct gacaggaaaa gaacataaac acgttttacg
     8401 agatattcgt aatatggtcg aagagcttaa cggggccaaa actgagcact gctcaacttt
     8461 gagcagcgag cttaacgggt ccaaatttgg gctcgttggc gaggaagtat acaaggacgc
     8521 gaaaggtgag agccgaacaa tgtatcgtct cgaccgtaag catactttca ttctggtcgc
     8581 tggctattcc gttcacctcc gcgccaagtg ttacgaccat attcagacgt tagagcgtcg
     8641 agtccttcaa ctggaagacc agaagaagcg agcagccatt cagtccgcta accgccgagg
     8701 cgtgacttgg ggcgactact gcaaaaccta tggtctgccc gctcagaagc taatgaccgc
     8761 acttctccag catcgtggcc tctttcgtaa gaacccaatc agtaacgagt ggtctgtaaa
     8821 tccgaagtat tccgactgct ttcgtatcat taagccgtca gaccagaagt tctcagcagg
     8881 tgggtataac ttccggttca acgctaaggg gctggaggtc ttcggtaagc ctgagatggt
     8941 tgacaagatg cgaggcatcc ttattgcatt cactggcacc gaccagcaga agcaagagca
     9001 cctcctgaag ctggcacaat ccggtaaggt ggagggaatc taagatgatg attgacccgc
     9061 gcacacctga ggggcgcatg actctccgtt accgtggcta ccggactgaa gtcctcctca
     9121 gagagcttgg tctagacccg gaagatgaga cccgccagca ccaatcccga gacgagctaa
     9181 tagcccaact ggtcgctatg aaactgccgc ttaaccgtta gtagctcacc tactcaatac
     9241 ctaatttaaa caccttttga gatgaccgat gcggggagac ccgtaggcct tactgtgcct
     9301 gcgataccga tagtctcaac tctcaagagg agaattacct atgatgttca acaacaacaa
     9361 ctggaagctg tcagtcaccg acatcaacct ttatgaaaac actgtgagtc tggacggtca
     9421 gtcctatccg ttgtcgctcg ctatcaagac cctcattccg ggctacctgt caggcttacc
     9481 gtc
//
LOCUS       DAJTRH010000128         9473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTRH010000128 DAJTRH010000000
VERSION     DAJTRH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..467)
                     /gene="pflA"
                     /locus_tag="OO339_003509"
     CDS             complement(<1..467)
                     /gene="pflA"
                     /locus_tag="OO339_003509"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR5928003.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAK"
     gene            complement(659..2941)
                     /gene="pflB"
                     /locus_tag="OO339_003510"
     CDS             complement(659..2941)
                     /gene="pflB"
                     /locus_tag="OO339_003510"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5928004.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(2996..3853)
                     /gene="focA"
                     /locus_tag="OO339_003511"
     CDS             complement(2996..3853)
                     /gene="focA"
                     /locus_tag="OO339_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5928005.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4259..6019)
                     /locus_tag="OO339_003512"
     CDS             complement(4259..6019)
                     /locus_tag="OO339_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5928006.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6149..6775
                     /locus_tag="OO339_003513"
                     /pseudo
     CDS             6149..6775
                     /locus_tag="OO339_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            6974..8062
                     /gene="serC"
                     /locus_tag="OO339_003514"
     CDS             6974..8062
                     /gene="serC"
                     /locus_tag="OO339_003514"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5928007.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8133..9416
                     /gene="aroA"
                     /locus_tag="OO339_003515"
     CDS             8133..9416
                     /gene="aroA"
                     /locus_tag="OO339_003515"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5928008.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2405 a         2267 c         2385 g         2416 t
ORIGIN      
        1 tatttagcga actccagcgt gcggtggttg gaaactccaa ccagattttg gtggatctcg
       61 tcgttcatct gtttgagatc gagcattacc aggtcggtta cttccagcag ttcatcaatc
      121 accggatcgt aacgacgaac aaaaccgttg gtatccagac aggtatgaat gccttctttt
      181 ttacaggcgc ggaaccagtc acgaacaaac tcagcttgca ggattgcttc accgccggat
      241 gcggtaacgc cgccgccgga agcgttcata aagtggcgat aggtcaccac ttccttcatc
      301 aaatcttcaa cggtaacttc tttaccgcca tgcgtgtccc aggtgtcgcg gttatgacaa
      361 tacaggcagc gcatcaggca gccctggaaa aaggtgataa agcgaatacc tgggccgtct
      421 acggttccac aggattcaaa ggagtgaatg cgaccaataa ctgacattgc ggtgtttctc
      481 cagatgtggc ccatctgagg ccgtgttggt gcgcagctcg aaggctacgt cgagtctgtt
      541 ttggcagtca ccttaaagta tagatagctg acaaaaaagg ctctcgtgct aaaaaaggcc
      601 ccactttcgt ggagccttta ttgtacgctt tttactgtac gatttcagtc aaatctaatt
      661 acatagattg agtgaaggta cgagtaataa cgtcctgctg ctgttcttta gtcagcgagt
      721 tgaaacgtac tgcgtagcca gatacacgga tggtcagctg cggatatttt tccgggtttt
      781 ccatcgcgtc gagcagcatt tcacggttca tcacgttaac gttcaggtgc tgaccacctt
      841 cgatggatgc ttcgtggtgg aagtaaccat ccatcagacc agccaggttg gtcttacgaa
      901 cttcgtcgtc tttacccagt gcgttcggaa cgatagagaa ggtgtaggag ataccatctt
      961 tagcgtaagc aaacggcagt ttagcaacgg aagtcagaga cgctacagca cctttctggt
     1021 cacgaccgtg catcgggtta gcacccggtc cgaacggcgc gccagcacga cgaccgtctg
     1081 gggtgttacc agttttctta ccatacacaa cgttagaagt gatggtcaga acagactgag
     1141 tcgggatagc gtcacggtag gtgtgcagtt tctgaatttt cttcatgaaa cgttctacca
     1201 ggtcaacagc caggtcatct acacgcggat cattgttacc aaactgcggg tattcgcctt
     1261 cgatttcgaa gtcgatagcc agaccgtctt cgtcacgaat cggtttaact ttcgcatatt
     1321 tgattgcaga cagggagtca gcagcaacgg acagaccagc gataccacac gccatggtgc
     1381 ggataacgtc acggtcgtgc agcgccatca gagaggcttc gtagctgtac ttgtcgtgca
     1441 tgtagtggat gatgttcagt gcagtgatgt actgtttagc cagccagtcc atgaagtgat
     1501 ccatgcgctc catcacttca tcgtagctca ggagatcgcc tttgatcggt tcagacttcg
     1561 gaccaacctg cattttcagt ttttcgtcaa cgccgccgtt gattgcgtac agcatggttt
     1621 tcgccaggtt tgcacgcgca ccgaagaact gcatttgttt accaacgatc atcgggctta
     1681 cgcagcaagc gatagcgtag tcatcgttgt tgaagtccgg acgcatcagg tcatcgttct
     1741 catactgcag agaagaggtg tcgatggaca ctttagcggc gaatttcttg aagttcagcg
     1801 gcagtttttc agaccacaga atggtcatgt tcggttccgg agacggcccc atagtgtaca
     1861 gggtgttcag gaaacggaag ctgtttttgg taaccagggt acgaccatcg aggcccatac
     1921 caccgataga ttcggttgcc cagattgggt cgccagagaa cagttcatcg tattccggag
     1981 tacgcaggaa gcgaaccata cgcagtttca tgaccaggtg gtcaaccatt tcctgcgctt
     2041 cttgttcggt gatcttgcca gctttcaggt cacgttcgat gtacacatcc aggaaggtgg
     2101 aggtacgacc gaaggacatt gcagcaccgt tctgagactt aacagcagcc aggtagccga
     2161 agtaagtcca ctggatagct tcctgagcgt tggtagccgg accagagatg tcgtagccgt
     2221 atttcgcagc catttctttc atctgaccca gagcgcggtg ctgttcagcg atttcttcgc
     2281 gcagacggat agtctgttcc aggtttacgc cgttttccag atcagcctgt agagaggtga
     2341 actgagcgta tttgtctttc atcaggtagt cgataccgta cagcgcaacg cgacggtagt
     2401 caccgatgat acggccacgg ccataagcat ctggcagacc ggtcagaaca ccggatttac
     2461 ggcaacgcag gatgtccgga gtgtaaacgt cgaacacgcc ctggttgtga gttttacggt
     2521 attcagtgaa gatttttttg atcatcgggt ccagttcgcg gttgtacgct ttgcaggaac
     2581 cttcgatcat tttgatacca ccgaacggga taagagcacg tttcagcgga gcttcagtct
     2641 gcagaccaac aactttttcc aacgctttat tgatgtagcc agcgtcgtga gaggtgatgg
     2701 tggaagcaac agcggtgtca aaatcaactg gcgcgtgagt gcggttttcc agtttaacac
     2761 cttccattac tttgtcccac agggtggtgg tcgcttcagt agcgccagcc aggaaggact
     2821 cgtcaccctc gtacggagtg tagtttttct gaatgaagtc acggacgttt acttcattct
     2881 gccagtcacc tttggtaaaa ccttcccagg ctgtggctaa cttttcatta agctcggaca
     2941 tgtaacacct accttcttaa gtggattttt tatttactgc gtacttcgac aaccattaat
     3001 ggtgatcgtt ttcacgcagg taaatgaccc agtatgtcaa cccaaccaac aaaccaccgc
     3061 cgataatatt accgatcgta accgggatca ggttatcagt gatgaagttc atcacggtca
     3121 ggtgagaaaa attttccggt gcagaaccga cagcggtcca gaattccgga gatgcgaagt
     3181 cgcggattac aatacccatc gggatcataa acatgtttgc gatactgtgc tcaaaaccgc
     3241 tggcaacaaa catcgcgacc ggcagcacca taatgaacgc tttgtccatc aggctgcggc
     3301 cagaataact catccatact gccagacata ccatcaggtt tgccaggata ccaagacaga
     3361 cggcctcaat aaaagtatgg tgcactttgt ggtcggcggt ttgtaggacg tttagtcccc
     3421 attgaccatt tgcggtcata tactcgccgg aaagccacat taaaagtaca aacagcagtg
     3481 cgccgaccag gttgccaaaa tagacattta gccagttttt agccaactga caccaggtga
     3541 tgcgcccact cgccttagca acaacaatca acactgtgga agtaaagaga tcggctccgc
     3601 agacaacaca aagaatcagc cccagagaga agcaaatgcc gccaaccagt tttgccatgc
     3661 cgaagggcat tgtgcctgtg ccagtggttg ctgtgatata gaagacgaat gcgattgaga
     3721 tgaaaacacc ggcggtaatc gccagataga aagtcttaag cggatgtttc gttgctttat
     3781 agacacccgc ctcttcggcc actttggcca ttgcagcagg aagtaaaaga tcaaaagggt
     3841 tgtcagcttt cacactaact ctctctttat taagtcggcg acgagatact aacaaagcat
     3901 tatagatgag aaattgatat agatcatatc tcgcctggct tataggcccg taactcgcat
     3961 ggtttttatg caaatacgga gtaactattt gattatccaa ataaaaataa attttaaaaa
     4021 ttaacaaatg agttgaattt tttccgcatc ctccgctaaa acagttaatt aaaagggagc
     4081 atcaggcgaa taaagtaaca atatcgatcg tatttattaa atacaaatta ccgatattta
     4141 accttataat tacaattatt ttattaatgc aaatatatgt aaagcggggc attaaaaaaa
     4201 cgccccgtaa tataactcag actaatcatt aagcctacat tgcgtaggct atttgatttt
     4261 attttgccca gaatgctgct ttggcgcgct gcagcttttc gtaggccttc aacaacgact
     4321 gatgtgcagc aaacgcgtgc agatcgctat ctaccggttg caggccgtaa aacgccgctt
     4381 cgccgctcat tgccgcactg gcggcttcta cggcatctgc gccgtacatg cgaacaaacg
     4441 cattcagata ttgcagcggc tggcgatctt cttcctgtgc cagtaataac aacgtttgca
     4501 ggcagcgata atagttggcg cgttccggac taaatactga tgagttaaac tccatcgtcc
     4561 attcggtcca gaccagagcc tgttccagat cgccaccagc cagcgccagc atggctttta
     4621 attcaccgat acgcagggtg taccaaccgt tatccgaccc ggtcgccaga cccaacagct
     4681 cacgcacgcg ggtaaagtca tcaaaacctt cttcatccag ttgctcgatg aggttcaggt
     4741 aatcttcttt ttcccactcg ctgcctggta gcgaaagaat cgtttcacgt aaatggctgc
     4801 ccatactgtt attcgcgagc cacagatctt cagccggata aatatcggac atgccaggca
     4861 cgataatacg gcaagcataa acgcccagat gctcgtaatc ggcaatataa acttctttat
     4921 cttctttgtt gaagatagcc atcagcgtag caaactcttc ttccgtggtg ccggagaaat
     4981 tccagtccac aaacggataa tcggcatcct gcttgaacag gtcccaggag attaaaccgc
     5041 tggaatcgat aaagtgcgtt tcgaggttgg tatgttcagc gacttcttca tcatcgaagg
     5101 ttggcggagt aaacacatcc aaatctttca ggccacgacc ttgcagcagc tcggtcacgg
     5161 tacgttccag tgctacgcca aaatcaggat gcgcaccgaa agaggcaaag caggtgccgt
     5221 ttgcaggatt aaacagtacc acgcaaatca ccggatactg gccgccaagg gaaccatcat
     5281 atgcgaagat cgggaaacct tctgtttcca gtgtttcgat cgcttcaacc accgctgggt
     5341 aacgcgccag cacgtctgcc gggatttccg gcaagctgat gctttcagca ataatgcggt
     5401 ttttcacgta gcgttcgaaa acttcggaca acccctgaac gcgtgcttcg ttgcgggtat
     5461 tgccagcgga cataccgtta gaaacgtaca ggttaccaat gatattcatc ggaatataaa
     5521 cggtctgatt gtcggactgg cgcgtaaacg gcagaccgca aataccacga tcttcgttac
     5581 cggattgtag gtcaatcagc atactgccgg tcaattcatt ctccggatcg taaaatgcgc
     5641 gcagacggtc atcgagcagc ccttctggca catcgtcatt ttcgctcagt gggaaccatt
     5701 tttcgttggg ataatgcaca aatggaccgt tggcgatggt ttcgcccagc cagaaatcag
     5761 caaaaaagta gttggttgag agacgctcga aatattcacc gagtgcagaa gccagcgccg
     5821 ctttcttggt tgcgccttta ccgttggtaa aacacagtgc gcactctttg tcgcgaatat
     5881 gtacagacca gacgttaggc acgggattca gccaggaggc ctcttcaatc tgaaagccga
     5941 ggtctgaaag tttttgctgg aagcgagcga tggaatcttc cagagcggca tctttgccgg
     6001 ggataaatgt ttgcgtcatg aaaatcactt tagtcgtacg gaaagcgcgc aataatacgg
     6061 gttttatctc aaaggcgcta tcaccgccgc catgccgatg aactgttgac tatgctttta
     6121 gcggataaac cacgttaaga gcataaaaat gaaggcgttc gatctccacc gtatggcatt
     6181 tgataaagtg ccttttgatt tccttggcga agttgcacta cgtagtcttt atacctttgt
     6241 actggttttt tgttcctcaa aatgaccgga agacgcggtg tgcgacagat gtcgctgttt
     6301 gaagtgttaa tcattctcac gctgggttca gcggcgggag atgtggcgtt ttatgatgat
     6361 gtgccgatgg tgccggtact tatcgtcttt atcactctgg cgttgttata ccgcctggta
     6421 atgtgatggc gagctggcct ggtcgaaact caataactcc aacatgacgg aatttgagtt
     6481 ctttatggag ctacgattgc gtggcgtgga gcagctgggg caggtacgtc tggcgattct
     6541 cgaaaccaac gggcaaatca gtgtctattt ctttgaagat gacaaggtga aaccgggttt
     6601 acttatttta cccagtgatt gtacgcagcg ttacaaagtg gtgccggagt cggcggacta
     6661 tgcctgcatt cgttgtagtg aaatcattca tatgaacgcg ggggaaaaac aattatgtcc
     6721 gcgctgtgca aatccagaat ggacgaaggc aagtcgggca aaacgggtga cctgacagta
     6781 aaaacatcgg ctttttgcta ataatccgag agattctttt gtgtgatgca agccacattt
     6841 ttgccctcaa cggttttact cattgcgatg tgtgtcactg aatgataaaa ccgatagcca
     6901 caggaataat gtattacctg tggtcgcaat cgattgaccg cgggttaata gcaacgcaac
     6961 gtggtgaggg gaaatggctc aaatcttcaa ttttagttct ggtccggcaa tgctaccggc
     7021 agaggtgctt aaacaggctc aacaggaact gcgcgactgg aacggtcttg gtacgtcggt
     7081 gatggaagtg agtcaccgtg gcaaagagtt cattcaggtt gcagaggaag ccgagaagga
     7141 ttttcgcgat cttcttaatg tcccctccaa ctacaaggta ttattctgcc atggcggtgg
     7201 tcgcggtcag tttgctgcgg taccgctgaa tattctcggt gataaaacca ccgcagatta
     7261 tgttgatgcc ggttactggg cggcaagtgc cattaaagaa gcgaaaaaat actgcacgcc
     7321 taatgtcttt gacgccaaag tgactgttga tggtctgcgc gcggttaagc caatgcgtga
     7381 atggcaactc tctgataatg cggcttatat gcattactgc ccgaatgaaa ccatcgacgg
     7441 tatcgccatc gacgagacgc cagacttcgg caaagatgtg gtggtcgccg ccgacttctc
     7501 ttcaaccatt ctttcccgtc cgattgacgt cagccgttat ggtgtaattt acgctggcgc
     7561 gcagaaaaat atcggcccgg ctggcctgac aatcgtcatc gttcgtgaag atttgctggg
     7621 caaagcgaat atcgcgtgtc cgtcgattct ggattattcc atcctcaacg ataacggctc
     7681 catgtttaac acgccgccga catttgcctg gtatctatct ggtctggtct ttaaatggct
     7741 gaaagcgaac ggcggtgtag ctgaaatgga taaaatcaat cagcaaaaag cagaactgct
     7801 atatggggtg attgataaca gcgatttcta ccgcaatgac gtggcgaaag ctaaccgttc
     7861 gcggatgaac gtgccgttcc agttggcgga cagtgcgctt gacaaattgt tccttgaaga
     7921 gtcttttgct gcaggccttc atgcactgaa aggacaccgt gtggtcggcg gaatgcgcgc
     7981 gtctatttat aatgccatgc cgctggaagg cgttaaagcg ctgacagact tcatggttga
     8041 gttcgaacgc cgtcacggtt aatgccgaaa ttttgcttaa tccccacagc cagcctgtgg
     8101 ggtttttatt tctgttgtag agagttgagt tcatggaatc cctgacgtta caacccatcg
     8161 ctcgtgtcga tggcactatt aatctgcccg gttccaagag cgtttctaac cgcgctttat
     8221 tgcttgcggc attagcacac ggcaaaacag tattaaccaa tctgctggat agcgacgacg
     8281 tgcgccatat gctgaatgca ttaacagcgt tagggttaag ctatacgctt tcagccgatc
     8341 gtacgcgttg cgaaattatc ggtaacggcg gtccattaca cgcagaaggt gccctggagt
     8401 tgttcctcgg taacgccgga acggcaatgc gtccgctggc ggcagctctt tgtctggata
     8461 gcaatgatat tgtgctgacc ggtgagccgc gtatgaaaga gcgcccgatt ggtcatctgg
     8521 tggatgcgct gcgcctgggc ggggcgaaga tcacttacct ggaacaagaa aattatccgc
     8581 cgttgcgttt acagggcggc tttactggcg gcaacgttga cgttgatggc tccgtttcca
     8641 gccaattcct caccgcactg ttaatgactg cgcctcttgc gccggaagat acggtgattc
     8701 gtattaaagg cgatctggtt tctaaacctt atatcgacat cacactcaat ctgatgaaga
     8761 cgtttggtgt tgaaattgaa aatcagcact atcaacaatt tgtcgtaaaa ggcgggcagt
     8821 cttatcagtc tccgggtact tatttggtcg aaggcgatgc atcttcggct tcttacttcc
     8881 tggcagcagc agcaatcaaa ggcggcactg taaaagtgac cggtattgga cgtaacagta
     8941 tgcagggtga tattcgcttt gctgatgtgc tggaaaaaat gggcgcgacc atttgctggg
     9001 gcgatgatta tatttcctgc acgcgtggtg aactgaacgc tattgatatg gatatgaacc
     9061 atattcctga tgcggcgatg accattgcca cggcggcgtt atttgcaaaa ggcaccacca
     9121 cgctgcgcaa tatctataac tggcgtgtta aagagaccga tcgcctgttt gcgatggcaa
     9181 cagaactgcg taaagtcggc gcggaagtgg aagaggggca cgattacatt cgtatcactc
     9241 ctccggaaaa actgaacttt gccgagatcg cgacatacaa tgatcaccgg atggcgatgt
     9301 gtttctcgct ggtggcgttg tcagatacac cagtgacgat tcttgatccc aaatgcacgg
     9361 ccaaaacatt tccggattat ttcgagcagc tggcgcggat tagccaggca gcctgaatga
     9421 acaacgggca ataaatagcc aaatctttct ttatcaaaac gtcggcacat tgt
//
LOCUS       DAJTRH010000129         9334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTRH010000129 DAJTRH010000000
VERSION     DAJTRH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="OO339_003516"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="OO339_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5928009.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="OO339_003517"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="OO339_003517"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5928010.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="OO339_003518"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="OO339_003518"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5928011.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="OO339_003519"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="OO339_003519"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5928012.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO339_003520"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO339_003520"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5928013.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO339_003521"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO339_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5928014.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO339_003522"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO339_003522"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5928015.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO339_003523"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO339_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5928016.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO339_003524"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO339_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5928017.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO339_003525"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO339_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5928018.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9181..>9334)
                     /locus_tag="OO339_003526"
     CDS             complement(9181..>9334)
                     /locus_tag="OO339_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928019.1"
                     /translation="IARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQ
                     KVVNED"
BASE COUNT         2389 a         2337 c         2371 g         2237 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg
     9301 ctgttccagc ttgcgtgctt tggcgcgggc aata
//
LOCUS       DAJTRH010000130         9329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTRH010000130 DAJTRH010000000
VERSION     DAJTRH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(139..1041)
                     /locus_tag="OO339_003527"
                     /pseudo
     CDS             complement(139..1041)
                     /locus_tag="OO339_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="OO339_003528"
     CDS             1101..1379
                     /locus_tag="OO339_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928020.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="OO339_003529"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="OO339_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5928021.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="OO339_003530"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="OO339_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5928022.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3533..3865
                     /gene="hdeA"
                     /locus_tag="OO339_003531"
     CDS             3533..3865
                     /gene="hdeA"
                     /locus_tag="OO339_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5928023.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3981..4307
                     /gene="hdeB"
                     /locus_tag="OO339_003532"
     CDS             3981..4307
                     /gene="hdeB"
                     /locus_tag="OO339_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5928024.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4371..5017
                     /locus_tag="OO339_003533"
                     /pseudo
     CDS             4371..5017
                     /locus_tag="OO339_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5059..5589)
                     /locus_tag="OO339_003534"
     CDS             complement(5059..5589)
                     /locus_tag="OO339_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5928025.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5745..6311)
                     /gene="slp"
                     /locus_tag="OO339_003535"
     CDS             complement(5745..6311)
                     /gene="slp"
                     /locus_tag="OO339_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5928026.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6558..7781)
                     /locus_tag="OO339_003536"
     CDS             complement(6558..7781)
                     /locus_tag="OO339_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928027.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8362..>9329)
                     /locus_tag="OO339_003537"
     CDS             complement(8362..>9329)
                     /locus_tag="OO339_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5928028.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2719 a         1857 c         1940 g         2813 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaag
     3301 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
     3361 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
     3421 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
     3481 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
     3541 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
     3601 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactca tggacctgtg aagatttcct
     3661 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
     3721 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
     3781 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
     3841 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
     3901 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
     3961 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
     4021 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gttggcagcc aatgaatctg
     4081 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
     4141 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
     4201 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
     4261 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
     4321 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
     4381 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     4441 aaatgcgcgg caaaggagca gggttacgca cacatgtatt aattggcatg ggaagcgccc
     4501 tgtttatgat tgtttcgaaa tatggttttg ctgacgtgct gtctttagat cacgtcggac
     4561 tcgaccccag ccgtattgct gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta
     4621 acattctcgt tcgtaaccaa aatattgtag gtctgacgac ggcagcggat atctgggtga
     4681 ccgccgcata ggtatggtta ttggcagcgg tatgtacgaa ctgggtattt atggttcagt
     4741 gatgaccttg ttggtgctgg aagtcttcca tcaattaacc ttccgcctga tgaacaaaaa
     4801 ttaccattta cagctgacat tagtgaacgg taatacggta tcaatgctcg actggtttaa
     4861 gcagcaaaaa ataaaaactg atttagtttc gttgcaggaa aatgaagatc atgaagtggt
     4921 cgccattgat atccagcttc atgccacaac gttgatagaa gacctgttgc gattattaaa
     4981 aggtatggca ggggtgaaag gcgtttcaat tagctaatct gtgtggcagc gtagccagac
     5041 tcaccgtaag cctgaaattc acaccagata atcaatatgc tgatggcgta ccagctcatt
     5101 gatccgctta agattgagga tcatcatgat attgtgttta tggcagtaga acgtttttag
     5161 cgatattttc agtttctcca gaatatcttc agttgtcatt tgattcgcaa tgcagctcag
     5221 aacctgatgt tgcttgcgcg aaagtgacac attcgcttca ggcacagagc agtaactgcc
     5281 attgatgagt gcgacaaaat caatgatagt gatagtccgc ggaacaaagg tcaccggtac
     5341 tttccccaga cagcgtttaa tattaaaggg ggagaaaaca atgacatgga caggttttaa
     5401 tcgttcaatt tgagtgagaa aattagaatg aaatacatta ttcattaatg tatcaataat
     5461 gacgaaggca ttctgatgca acattacgtc gatctcttct tcgttctgta tatgaacgac
     5521 attaccttta ctcagaatgt ttttcatcgc ggtgaagaac atcgtatccc tggtaattat
     5581 aagaaacata accgactcct ggtgcggaca ttaactttat aatcagtttg ccaaaggtaa
     5641 ttaacaggac agggcttata ataataatag ttagcttact gtatattaat tttatgtaat
     5701 acatgatgca gatgctgaga aagcatctgc atctttcggt ggtgttattt gaccagctca
     5761 ggtgttacct gactcaccgc attggtgtag taaggcgcac cccagcccgg ttccggccag
     5821 aatgcaccat agccgtaatc ccacaggtta taggtggtat taaccacttc tctcaaatgc
     5881 cacacctgga tgccctgcat attcacttcc aggaagttat acgggacttt attgataaag
     5941 cctggttgtt caccctgaat ggtaccgagg atggtaacaa agtgattacg atagttcact
     6001 ggatcaagga agccgctttg tctggcgagc agtcggccct gatagttggc ttcaatatca
     6061 ggcttcgcat agctatccaa cggtaataca gcgatttcta acaacgtatc cgttttgcca
     6121 ttgataacgt tgataacctt cccaccaaag cgcgcttgtt gaccaacata taaccccggc
     6181 tggttatgaa cagcaacaaa acttttttga atatcaggtt ggttattgcc tttgatattt
     6241 tgcggaattg aactacatgc ggcaagcaaa aatgaaaggc tgaggatgag tgcacctttt
     6301 gtcatgttca tgttactatc cttatcaaca aatattaaaa ccataataga tatttcttta
     6361 tagttttcat ctgattctga ggtgatatgc ctgatagtaa cgtaaacctg tctgatgttt
     6421 atatcccttc tgtttagttc aattccgtta ttttggggcg tggtatgagt atccatcgcc
     6481 caataatata accctttgtt ttaaaattac ataaaatcta tttttattca atgattataa
     6541 agtgctaata atcaatatta tggtattata ttctgataat gatttgataa gcgaattctt
     6601 ttgccagatg gtatttctat ttccacccca taatttatat ctttttcatt aggcagatct
     6661 ctctttaaat aaaaggggta tgaaatcttt tcgggttgaa gtgtctcgta atgaaattct
     6721 atgaattcat ttttagacat tgttgcccat ttttgaattt tattgtatag agagggtgtt
     6781 gtaaactctt gaagttgttt ataacatatt tttttgaact cttgcttata ttttaatatg
     6841 tcatcaaatg aaagattcac tacctttgcc tctaagaaat caattattct cgaagcaatt
     6901 tccgctttcg cgcgtaaaga gttgtcatta atagttaaca atgtagaaaa caaaccattg
     6961 atttgtttgt caagatgttg ttcatattta tgtagtttgg ggatgggatt ttccttgtgt
     7021 gaaaaaatag tgttaaataa atttgcgtca gtgaaattta tattaagtag atcttcaaaa
     7081 taaaattttg ataaggttag gactagcttg gacatatctt gccctgtcat gttgaaatca
     7141 ataaagtcgc atccagcaag ggcttccatc ctattttcat ttttactgtg ctcaatatac
     7201 agcttcgcca tttttaagta caagctttga atgtctctta tatcagttgg taagtcagct
     7261 cgttgcccag caaaattttt attatcggaa gaaacggttt ggacactgaa tctttcgggt
     7321 attccttctt ctacgagaat tttagttatg attggattgt tgggttctgg attaacgaca
     7381 acaatgattt gacatacaga atccttatcc ttttcgtatc taatgttatg aatctgatta
     7441 agtattgatt gtgtctgttg cgtaagtggt gtcgaactta tcgtttcaat gttattaata
     7501 agacttacaa gttgaaccac aacttttgag tagtctgcat ttgtatatgt ggtgttcgtg
     7561 tggcatgcat taatttgttg gagttgttgt tgcgctaaat caaattgttc agggttgtat
     7621 ccttctccag agaatgtgga attaatgggt tgttgttctt cccaattatc acaaggccac
     7681 ttcggtgtgt ctggcgtttt aacattatcg gtatttgttg tgcagttata aatatcgccg
     7741 tgatatgtat ttattatacc tggggtaaag tcaattgaca taacattcct tattcatcat
     7801 ctgttttatg ctaatgtttt taaattggat aattagaaca ggaatggatt ttcttctaca
     7861 cacaataaat ataatgcgtt atatcttcct gatataatta cctttaaatt ccctttcatt
     7921 tccacccggt aatatttgtg tataaaaaaa gggcagtgat agtatcgtaa aggaaaacgt
     7981 taacaactat caaaaaacgc tcattttaaa aagttattat gttctccgat agtgtgtgaa
     8041 gtaatatttt tgtgatgttt tagatgtgta ataatgaacc aggttaaagt gtatttaaaa
     8101 atgtgttgta ttagcgactg aaatctgcct tttaaaaagg aggagggtac tgagacaaag
     8161 agtcattgct tctccgataa aatgtcagag aagcatgatt acgtggttat tgtctgttga
     8221 tgacttgttg accgagccgt catcattctc aatgcattag ttaaatcatt tgtgattttt
     8281 aaggattatc ctgtctatga ttttctactt agaaggatat ggcactaact atgcatctta
     8341 tagtttactg ctggcatcta tttaaaatag caattgagtc aaatagccca tcaagatagc
     8401 catacctaat ataaccgtca ggaatgcagc tatcatcggc attctgaaca ttgatttaag
     8461 taatatcact tccgtcaggc ttgcgccggc actgccgatg attaatgcca ttaatgctcc
     8521 cagacccatt ccttttgtca tcagaacaga tgccagaggg ataaccgcct cagcccggat
     8581 atacagcgga ataccgacaa cggcgcttaa tgggatggcg aagggattat ctgctcctgc
     8641 atgagcggca atccacgctg agggaataaa gccataaata aaagatccta ttaatactcc
     8701 taacaaaaga tatggcagta catctttaaa ctgttgcaac gcatctttgg tggctagttt
     8761 tatgggatta atacttacta cagctttggt actgcaacag gtaactacgg ggttctcaat
     8821 ggctttagcc gctggactac aacaactcac ttttatgggc gtatatgtcg ttcccggcga
     8881 agtttttgct ggagcacaac aatttgctga tgagctttta ctggcaatga tatgacgttc
     8941 aaaccccaga gaatccagga taatactggc aagaacggag acgccggctg cgataatcgc
     9001 gtacaacaag gtgactttcc aaccaaaggt catccacatt agtccgacga taatgggatt
     9061 aagtaatggt gaaacaaata aaaaagtgag agtcggacca aacccggctt tcgctgacaa
     9121 caatccacgt aacatgggta ttgtcgagca actacagaac ggggtaacgg ctcccaacag
     9181 agcagccagg agatagcctc tcccttttct ggcccccatc atctgctgga ttttatggtc
     9241 tggcaccttt tgtcttatca ggctgacacc ggcactaatc actataaaca gcaacgaaag
     9301 ctcgacggcg agaaacacaa acatctctg
//
LOCUS       DAJTRH010000131         9306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTRH010000131 DAJTRH010000000
VERSION     DAJTRH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            11..1129
                     /locus_tag="OO339_003538"
     CDS             11..1129
                     /locus_tag="OO339_003538"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5928029.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1195..1443
                     /locus_tag="OO339_003539"
     CDS             1195..1443
                     /locus_tag="OO339_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5928030.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1508..1876
                     /locus_tag="OO339_003540"
     CDS             1508..1876
                     /locus_tag="OO339_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5928031.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            1970..2623
                     /gene="nfsB"
                     /locus_tag="OO339_003541"
     CDS             1970..2623
                     /gene="nfsB"
                     /locus_tag="OO339_003541"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5928032.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            2731..3978
                     /gene="ybdG"
                     /locus_tag="OO339_003542"
     CDS             2731..3978
                     /gene="ybdG"
                     /locus_tag="OO339_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5928033.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4046..5422)
                     /gene="pheP"
                     /locus_tag="OO339_003543"
     CDS             complement(4046..5422)
                     /gene="pheP"
                     /locus_tag="OO339_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5928034.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5523..8666)
                     /gene="cusA"
                     /locus_tag="OO339_003544"
     CDS             complement(5523..8666)
                     /gene="cusA"
                     /locus_tag="OO339_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5928035.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(8678..9196)
                     /gene="cusB"
                     /locus_tag="OO339_003545"
                     /pseudo
     CDS             complement(8678..9196)
                     /gene="cusB"
                     /locus_tag="OO339_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2253 a         2574 c         2299 g         2180 t
ORIGIN      
        1 agagaaacct atgccattac ccgattttca tgtttctgaa ccttttaccc tcggtattga
       61 actggaaatg caggtggtta atccgccggg ctatgactta agccaggact cttcaatgct
      121 gattgacgcg gttaaaaata agatcacggc cggagaggta aagcacgata tcaccgaaag
      181 tatgctggag ctggcgacgg atgtttgccg tgatatcaac caggctgccg ggcagttttc
      241 agcgatgcag aaagtcgtat tgcaggcagc cgcagaccat catctggaaa tttgcggcgg
      301 tggcacgccc ccgtttcaga aatggcagcg tcaggaggta tgcgacaacg aacgctatca
      361 acgaacgctg gaaaactttg gctatctcat ccagcaggcg accgtttttg gtcagcatgt
      421 ccatgttggc tgtgccagtg gcgatgacgc catttatttg ctgcacggct tgtcacggtt
      481 tgtgccgcac tttattgccc tttccgccgc gtcgccatat atgcagggaa cggatacgcg
      541 ttttgccccc tcacgaccga atattttttc cgcctttcct gataatggcc cgatgccgtg
      601 ggtcagtaac tggcaacaat ttgaagccct gtttcgctgt ctgagttaca ccacgatgat
      661 cgacagcatt aaagatctgc actgggatat tcgccccagt cctcattttg gcacggtgga
      721 ggttcgggtg atggataccc cgttaaccct tagccacgcg gtaaatatgg cgggattaat
      781 tcaggccacc gcccactggt tactgacgga acgcccgttc aaacatcagg agaaagatta
      841 cctgctgtat aaattcaacc gtttccaggc ctgtcgctat ggtctggaag gcgtcattac
      901 cgatccgcac actggcgatc gtcgaccgct aacggaagat accttgcgat tgctggaaaa
      961 aatcgcccct tcagcacata aaattggcgc atcgagcgca attgaagcac tgcatcgcca
     1021 ggtcgtcagc ggtctgaatg aagcgcagct gatgcgcgat ttcgtcgccg atggcggctc
     1081 gctgattggg ctggtgaaaa agcattgtga gatctgggcc ggtgactaag ccgggaattg
     1141 ccttacagaa gaatatccct gacaatggtg ttttaactta catttaacaa tgttatgaaa
     1201 caccctttag aaaccttgac caccgcagca ggcattttgc tgatggcttt cctctcttgc
     1261 ctgctgctgc ccgcccccgc actggggctg acgctggcac aaaaactggt gaccatgttc
     1321 catctgatgg atcttagcca gctttacact ttattgtttt gtctgtggtt tttagtgctg
     1381 ggcgctattg agtattttgt tctgcgcttt atctggcgac gctggttctc gctggcggat
     1441 taaacgtcga ttatcgccga acttcgcggc atactttgct tattctcctt cgcctaaagg
     1501 aacgtttatg gataagcaat cactgcacga aacggcgaaa cgcctagctc ttgagttacc
     1561 ctttgtcgag ctttgctggc cttttggccc ggagttcgat gtttttaaaa ttggcggcaa
     1621 gatttttatg ctgtcgtcgg agctacgcgg cgtccccttt atcaatctga agtccgatcc
     1681 acaaaaatcc ctgttaaatc agcaaatata cccaagcatt aagccagggt atcacatgaa
     1741 taaaaagcac tggatttcgg tgtattccgg cgaggaaatc tccgaagcgt tacttcgcga
     1801 tctgatcaac gattcgtgga atctggtggt tgatggtctg gctaaacgcg atcaaaaaag
     1861 agtgcgtcca ggctaaagcg ggaatctata gcgcattttt ctcgcttacc atttctcgtt
     1921 gaaccttgta atctgctggc acgcaaaatt acttacacat ggagtcttta tggatatcat
     1981 ttctgtcgcc ttaaagcgtc attccactaa ggcatttgat gccagcaaaa aacttacccc
     2041 ggaacaggcc gagcagatca aaacgctact gcaatacagc ccatccagca ccaactccca
     2101 gccgtggcat tttattgttg ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc
     2161 tgccggtaat tatgtgttca acaaacgtaa aatacttgat gcctcgcacg tcgtggtgtt
     2221 ctgtgcaaaa accgcgatgg acgatgcctg gctgaagctg gttgttgacc aggaagatgc
     2281 cgatggccgc tttgccacgc cggaagcgaa agccgcgaac gataaaggtc gcaagttctt
     2341 cgcctatatg caccgtaaag atctgcatga tgatgcagag tggatggcaa aacaggttta
     2401 tctcaacgtc ggtaacttcc tgctcggcgt ggcggcgctg ggtctggacg cggtgcccat
     2461 cgaaggtttt gacgccgcaa tcctcgatgc agaatttggt ctgaaagaga aaggctacac
     2521 cagtctggtg gttgttccgg taggtcatca cagcgttgaa gattttaacg ctacgctgcc
     2581 gaaatctcgt ctgccgcaaa acaccacctt aaccgaagtg taattctctc ttgccgggca
     2641 tctgcccggc tatttcccct cagattctcc tgatttgcat aaccctgttt cagccgtcat
     2701 cataggctgc tgttgtataa aggagacgtt atgcaggatt taatatccca ggttgaagat
     2761 ttagcgggta ttgagatcga tcacaccacc tcgatggtga tgattttcgg tattattttt
     2821 ctgaccgccg tcgtggtgca tattattttg cattgggtgg tactgcggac cttcgaaaaa
     2881 cgtgccatcg ccagttcacg gctttggttg caaatcatta cccagaataa actcttccac
     2941 cgtttagctt ttaccctgca ggggattatc gtcaatattc aggcggtatt ctggctgcaa
     3001 aaaggcaccg aagcggcaga tattctgact acctgcgcgc agttgtggat catgatgtat
     3061 gcgctgcttt cagtcttctc gttgctggat gttattttga atctggcgca gaaattcccg
     3121 gcggcatctc agttaccgct gaaagggata tttcagggga ttaaactgat cggcgcaatc
     3181 ctggtcggca ttttgatgat ctcgctgctg attggtcagt cgccagcgat tctaatcagc
     3241 ggtcttggtg caatggctgc cgtgctgatg ttggtattta aagatccgat tcttggtctg
     3301 gtggcaggta ttcagctttc cgcgaacgat atgctgaaac tgggcgactg gctggaaatg
     3361 ccgaaatacg gcgcggatgg cgcggtgatc gatattgggt taaccaccgt caaagtgcgt
     3421 aactgggaca ataccattac cactattccc acctggtctc tggtttctga ctcctttaaa
     3481 aactggagcg ggatgtcagc atctggcggg cgacgtatta agcgcagtat cagtattgat
     3541 gtcaccagta ttcgttttct tgatgaagac gaaatgcaac gtctgaataa agcgcatttg
     3601 ttaaagcctt atttaaccag ccgccatcag gaaattaatg agtggaatcg ccagcaaggt
     3661 tctacggagt cggtattaaa cctgcgccga atgaccaata ttggaacctt tcgtgcctat
     3721 ctgaacgaat atctgcgtaa ccatccgcgg attcgtaaag atatgacctt aatggtacgc
     3781 caactggctc caggtgataa cggtttaccg ctcgagatct acgcgtttac taacaccgtg
     3841 gtgtggctgg aatatgaaag cattcaggct gatatattcg atcacatatt tgcgattgtc
     3901 gaagagtttg gtctgcgact tcatcagtcg ccaaccggca atgatattcg ctctctggcg
     3961 ggtgcattta agcagtaatt aaaaaaaccg ctctcatcga atggatgaga gcggacatcg
     4021 ttttgtcgga tgtagcgtga atgccttatt tccgacgcag cgttttaaat gccacaaaca
     4081 ggaatacaat ccacaccggc agcaggatcg ctgacaagcg catatcatcc atcgtgcaca
     4141 tcagcagcaa aatcatgccg aggaaggcga tgcaaagata gttgccgaac ggatagagca
     4201 gcgccttaaa ctgtgtttca cgcccctgac gtcgcatcgc cgcgcgaaaa cgcagatgcg
     4261 ccaggcagat cataatccag ttcaacagca acgttgctac caccagcgcc atcagcagac
     4321 caaacgcttt ttgcggcagc agatagttga ttaacaccac cagcgaagtg atcgctccgg
     4381 aaagcatcag cgagttaatc ggcacaccgc gacggctgac gcgagtcaaa aacttcggcg
     4441 cattaccctg cacagaaagg ccaaacagca tgcggctgtt agagtaaacc ccgctgttat
     4501 acaccgacag cgatgccact agaatgacga agttcagcgc agaagctacc acattgctgt
     4561 cgagattatg gaaaatcatc acaaacgggc tactgttgga tttcacttcc acccacggat
     4621 agagtgccag taaaaccacc agtgaaccga tataaaacag caggatgcga tacaccacct
     4681 gatttaccgc ttttggaatg cttttttccg gatcgcgcgc ttcagcggct gtaatcccaa
     4741 tcagctccag accgccgaag gagaacataa ttaccgccag cgacaaaatc agcccattcc
     4801 agccggtggc gaagaaacca ccgtagcgcc agaggttgtc gatactggct ttctcgccgc
     4861 cgtaaccaga aaacagcagc cacaggccaa agccgatcat accgatgatc gccagcactt
     4921 taatcagcgc aaaccagaac tcggtttcgc catataagcg cacgttcacc aggttaacgg
     4981 cgttgatgat aataaagaag gcggcagccc aaatccacgt tggaacatcc gggaaccagt
     5041 actgcatata gatgcccgca gcggtcagct ctgccattcc caccagcacg aacattaccc
     5101 agtagttcca gccagagagg aagcccgcaa acggtcccca gtatttatag gcaaagtggg
     5161 caaatgaacc ggataccggc tcttcgacca ccatctcgcc aagctggcgc ataatcagga
     5221 aagcgatgat cccggcgacg ccgtagccca gcaatacagc cggacccgcc atctgaatcg
     5281 ccgggccaat gccaagaaac agaccagtac caattgcgcc acccagcgca atcagttgaa
     5341 tatgacggtt atgtaatccg cgatgaagcg tcggctcttg attcgacgca gtatcttccg
     5401 atacggttga cgcgtttttc acgcctttcc cctgtgtgtc ttttttgttg aggggcacct
     5461 tttaacattt agtgcccatc gtcgcaagac acaatccaca cggttaaacg aggtatcctg
     5521 ctttaattcc gtacccgatg tcggtgcagc cacatcagct tatacgccgc cgggataata
     5581 aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca tcggtgcggc aatccggctc
     5641 atcacctctg aaccagcccc cgttccccac agaatcggca gcagaccggc gataatcacc
     5701 gccaccgtca tcgctttcgg gcgcacgcgc aggaccgcgc cgtgatacaa cgcctcatcc
     5761 agcttctgct cgctgaatgt ttgtggatta ttcaacgacg gctcggcctc tatggcgtga
     5821 cgtaaataca tcagcatcac cacgccaaat tcggcggcga ccccggcaag ggcaataaag
     5881 ccggtacccg tcgccacaga aagatgaaag cccatccacc acagtaacca gatgccgccc
     5941 accagcgcaa acggtacgct gctgataatc agcaacgctt cgcccacccg acggaatgcc
     6001 agatacaaca gcacgaagat gatcatcagt gtcatcggca ccatgagttt cagcttatgg
     6061 ttggcgcgct ccagtagctc gaactgcccg gagaatgcca cgctggtgcc cggttttaac
     6121 tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca ccgacaccat gtcacgatcg
     6181 cgggcatcga tataaatcca gctcgtcggg cgcgcattct cggttttcag catcgacggt
     6241 ccggtagaga ctttaacgtc ggccacgtct gccagggtga tttgctgctt catcggcgtc
     6301 aggatcggca actggcgcag tgcctgcgga ctatcgcgcc agctttgcgg ataacgcaga
     6361 ttaattggat aacgggcaat cccttccacc gtttcgccaa ccatcgcccc gcccaccgca
     6421 gaagtcacaa acaactgcac atccgccacc gtcataccgt aacgtgcggc tttttcacgg
     6481 ttaatctcaa cgttgatata gcgcccacct tccagccgct cagcaagcgc agaagctacg
     6541 cctggcaccg ttcgcgccac ttcttcaatt tgctcagcca tcgcgtcgat atccgccagc
     6601 acagtgccgg aaactttaat gccgatgggg cttttaatgc cggttgagag catatcgata
     6661 cggttacgaa ttggcggcac ccacagattc gccagccccg gcagacgcac ggtgttatcc
     6721 agttcctcaa tgattttgtc cattgtcatg cctggccgcc actgatcctg tggcttaagc
     6781 tggatggtcg tttctaccat ctccagcgga gcagaatcgg tggcggtttc cgctttcccg
     6841 gttttgccaa atacccgcgc cacttcaggt acgctcataa ttagcttgtc ggttttttgc
     6901 agcatactcg ccgcctctgc tgcggaaatc cccggcagcg tcgatggcat atacaacaag
     6961 tcgccttcat tgatctgcgg taaaaattcc ccgccaactt tattgagcgg ccagagaacc
     7021 gtcagcaccg aaagcgccgc caccagcagc gtggttttcg gccagtgcag tactttcagc
     7081 aacagcggat gataaacacg aatcaaaaag cgattgagcg gattactgct ttccggcgga
     7141 attttgccac ggatccagta acccatcagg atagggatca ctacgatcgc cagcagcgcc
     7201 gcacccgcca tcgcatacgt tttggtgaac gccaacgggc caaacagacg cccttcctgc
     7261 ccttccaggg tgaagatcgg gataaacgac aacgtgataa tcagcagact gataaacagc
     7321 gccggcccca cttcaacaga cgcatcggtg atcacctgcc agcgcgtttt attatccagc
     7381 gtggcgtcag gatgctggtg ctgccactct tccagctgtt tatgcgcatt ctcgatcatg
     7441 acgatagcgg catcgaccat cgccccgacg gcaatcgcaa tgccacccag cgacataata
     7501 ttggcattca gcccctggaa gggcatgaca ataaaagcaa tacacaaccc cagcggcaac
     7561 gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa acggcgcgca gactaccgcc
     7621 accacaataa actcttccag caacttgccg ctgaggttgt cgatggcccg gtcaatgagc
     7681 tggctgcggt cgtatgttgt aactatctcc acgccttccg gcagactact tttcagcgtt
     7741 tccagtttgt ccttcacggc ggcgatcact tctcgggcgt ttttgccgga tcgcaggatc
     7801 accaccccgc ccgccacttc gccttcgccg tttagttcgg caatgccccg gcgcatcttc
     7861 gggccaatct ggaccttcgc aacatcgcgc agataaacgg gcacgccatt ttcactggct
     7921 tttaaaacga tgtgattaaa gtcgtcgagc gtttgtagat agccgctggc gcgcaccata
     7981 tattctgctt ccgccagttc gattgacgaa ccgcccgctt cctggtttga agcatccagc
     8041 gcgcttttta cttcggcgag actgatgcca tactgcgcca ggcgctgagg atcgataacc
     8101 acctgatact ctttcaccac accgcctacc gacgccactt ccgcaacgtc aggaatggtt
     8161 ttcagctcat atttgagaaa ccagtcctgc aatgagcgta aatcggcaag atcgtgctta
     8221 ccgctgcgat ccaccagtgc atattcatag atccagccaa cacccgtggc atctggtccc
     8281 agctcggcgc tgactcccgc aggcagctta ccctgtacct ggttgaggta ctccagcacc
     8341 cgcgaacgcg cccagtacgg atcggtgcca tcttcgaaaa tgacatacac ataagagtcg
     8401 ccaaactgcg agaaaccacg cacagtcttc gcgccaggca ccgacaacat ggtggtggtt
     8461 agcggataag tcacctgatt ttcaacgatt tgcggtgcct ggccgggata gctggtttta
     8521 ataatcacct gcacatcgga gagatccggc aacgcatcca ctggcgtatt aatgatggtc
     8581 caggtgcccc agatgctcag aaacaacgcg cccatcagca ccaaaaaacg gttcgccacc
     8641 gagcgacgaa taatccattc aatcattggt tattccctca atgcgcatgg gtagcacttt
     8701 cagagcgcat ccgctccagt gcgccagaaa tattggcttc ggaatcaatc aggaacaggc
     8761 cgccggaaac caccttttca ccttccgcca gaccagagcg taatgcggtg acgccttgcg
     8821 atgcctggaa aacagcaacg cgtttcggta caaagcgccc gtcggcatca acggtaatca
     8881 cccgctgttc gttgccggta tcaatcagcg cctgtgacgg aatgagcagc atcggttcgc
     8941 tggcggtgtt gagttgcaac caggcgttca tgcccggttt tagcgcctcg tcggcgttgt
     9001 cgacttccag acgcagctgc agcgtgcggg tcgcggcatc cacgccaggt agcagcgtcc
     9061 atttgcggat ggtgagtgtt ttatccggtc gcgccggaac ggtgagggta aactgcgagg
     9121 catctttcac cagccaggcg atggactccg ggatcgcagc agtgacccac accgggtcca
     9181 taccctgaat tcgcgattac gcgcaatccc cctgagaacc gccatgaaca agggtttcat
     9241 gatttgtgat tttagtttgg tacgcaattt ggtacacaac actattttca cttcagcggg
     9301 tagtca
//
LOCUS       DAJTRH010000132         9271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTRH010000132 DAJTRH010000000
VERSION     DAJTRH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            39..1154
                     /locus_tag="OO339_003546"
     CDS             39..1154
                     /locus_tag="OO339_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928036.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="OO339_003547"
     CDS             1833..2051
                     /locus_tag="OO339_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5928037.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /locus_tag="OO339_003548"
     CDS             2269..2511
                     /locus_tag="OO339_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928038.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO339_003549"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO339_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5928039.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO339_003550"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO339_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5928040.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO339_003551"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO339_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5928041.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO339_003552"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO339_003552"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5928042.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..>9271
                     /gene="fdhD"
                     /locus_tag="OO339_003553"
     CDS             8558..>9271
                     /gene="fdhD"
                     /locus_tag="OO339_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5928043.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFA"
BASE COUNT         2269 a         2280 c         2405 g         2317 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc g
//
LOCUS       DAJTRH010000133         9243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTRH010000133 DAJTRH010000000
VERSION     DAJTRH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO339_003554"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO339_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5928044.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO339_003555"
     CDS             1828..3147
                     /locus_tag="OO339_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5928045.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO339_003556"
     CDS             3445..3486
                     /locus_tag="OO339_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928046.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO339_003557"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO339_003557"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5928047.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO339_003558"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO339_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO339_003559"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO339_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO339_003560"
     CDS             5815..6711
                     /locus_tag="OO339_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5928048.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO339_003561"
     CDS             6708..7682
                     /locus_tag="OO339_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5928049.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO339_003562"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO339_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO339_003563"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO339_003563"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5928050.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2260 a         2257 c         2317 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 aga
//
LOCUS       DAJTRH010000134         9243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTRH010000134 DAJTRH010000000
VERSION     DAJTRH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..563)
                     /locus_tag="OO339_003564"
     CDS             complement(<1..563)
                     /locus_tag="OO339_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5928051.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLL"
     gene            complement(630..1355)
                     /gene="fimC"
                     /locus_tag="OO339_003565"
     CDS             complement(630..1355)
                     /gene="fimC"
                     /locus_tag="OO339_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5928052.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1392..1931)
                     /locus_tag="OO339_003566"
     CDS             complement(1392..1931)
                     /locus_tag="OO339_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5928053.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(1996..2544)
                     /gene="fimA"
                     /locus_tag="OO339_003567"
     CDS             complement(1996..2544)
                     /gene="fimA"
                     /locus_tag="OO339_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5928054.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3025..3621)
                     /gene="fimE"
                     /locus_tag="OO339_003568"
     CDS             complement(3025..3621)
                     /gene="fimE"
                     /locus_tag="OO339_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5928055.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWPQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4099..4701)
                     /gene="fimB"
                     /locus_tag="OO339_003569"
                     /pseudo
     CDS             complement(4099..4701)
                     /gene="fimB"
                     /locus_tag="OO339_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6155..6871
                     /gene="nanC"
                     /locus_tag="OO339_003570"
     CDS             6155..6871
                     /gene="nanC"
                     /locus_tag="OO339_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5928056.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6891..7997
                     /gene="nanM"
                     /locus_tag="OO339_003571"
     CDS             6891..7997
                     /gene="nanM"
                     /locus_tag="OO339_003571"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5928057.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8062..9042
                     /locus_tag="OO339_003572"
     CDS             8062..9042
                     /locus_tag="OO339_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5928058.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2713 a         2013 c         1945 g         2572 t
ORIGIN      
        1 ttgagcaatc cggcattaat accgggatcc cataactcag gaggaatata accacgcgcg
       61 cgattactca taaatgcctg agggatcgtc aggttcagtc gctgctgacc aacatccaga
      121 tgcgcagtag cgtcctggac cattgtggtt aatggcacac aggcatcatc cgccagcaga
      181 ttcataccgg cgacagaagc cgtattcagc cccatactgg cgagttgcgc gcgtgtcagg
      241 cagggaacaa tcccttgttc actgtcgccc gtattaaatg tgacatcacg cgttgccata
      301 taaccattat tcaaatagat atcgacgcga tacgtccctg gcggtaattc ttgcccattt
      361 tcaaaacgcg ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg cagattaaaa
      421 tagaggtcgg cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac aacgagtcgg
      481 acaaaaaaac cagccaaacg atgcttacga atatgcaagc attgtgtgtt tcgctggtaa
      541 agtcttaaat tcagatatga cattattcct ccggtttaaa atcacccggc agtcaattct
      601 ttttattcag gcgaaaaatt ccccctgcgt tattccatta cgcccgtcat tttgggggta
      661 agtgcgccat aatcatttat tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat
      721 ttaaccgtgc tttcgcccat tggaggcacc aatgcatttt caagaacccg ggttccggca
      781 ttcaactctg ttaccgtcag gtaatagggt gtcgggttaa tcagcgtcag agaattcgcg
      841 ctacgacgaa atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg
      901 cgatagtaca gtttaatgcg gctgataatt gcgagctgta gcatattctc agtcaatttt
      961 gatttatcca ttgacggaat cgctttaacg ttcatccaga ataaactttc ccggtcctgt
     1021 ggcaattggt tatttgttgc atcaagaata cgtaaggtat tctctttttt tcccttcatc
     1081 gcaaacagag gaggcgtcac gataaaacga ccatccttta caccatcggc attttccacc
     1141 catgattgaa ttaaataggt actattttca tcattatttg tcacggcaag ttgcacttgt
     1201 ttttgccctg ccggataaat tacgcgagtc gcacctaagg ccactcctgc ttcagcgcgt
     1261 ccggcaacca tcattgccat gaacatcagg atacctgcca gcaagcagaa tgttatttcc
     1321 tgcgatttcc ttacattgac gtttttatta ctcactgtcc tgttcctgtt atcacattat
     1381 ctactgaaca attactgata ggttaaagag aaccaggcct gggcattggc gatgccgcca
     1441 gtaacccgac gcccggtagc acgatatttg gcgatgaaat gtagcgaagt agagcctgag
     1501 taaagccgtt tccagtttgc tggaggacga ttaatcggta cgaggtttcc ttcatcatca
     1561 aacaacgcta caccaatatt ggaggctatc cctggcccct ctcccacgga gagcacatcc
     1621 ggatttttac catccgcgac accgtgaaac gccacaccca cacgttcact caccaccgtg
     1681 ctacattccc gtaaatgaat aacaaaaggc accggtgcgc tatcttcccc tgccgcatga
     1741 aaccggttac tgctgatttg ccccatattg accgtcattt gtttatcacc ggcttcaatc
     1801 cggcaagttt ccgcaataat gacgccctga aattgcatat ttccgccggg cagcgtggta
     1861 ttccatttat ttccggccag agcaaacatt ggcagcaacg acgctaacag aaataatctc
     1921 ttccttttca tcgttatttt tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc
     1981 ttgaacctgg gtaggttatt gatactgaac cttgaaggtt gcatccgcat tagctgcacc
     2041 cggggttgcc tcgccgattg cataataacg cgcctggaac ggaatggtgt tggtaccgtt
     2101 attcagggtt gtttgtgcac taaatgtcgc accgtccagc gtcagagcat tgcctgttct
     2161 gtctaggatc tgcacgccaa cgtttgttgc actacctgca gcagaactct gcagagccag
     2221 tacatcagta cgcgtagcat caattgccgt acctaagaag gcaacagcgg cttttgtggc
     2281 aacagtggta tcgcaatcat tcagctgaat gttaaaacca acggcagagc tggttgctcc
     2341 agcctgcttc aggctagcgg tacgaacctg tcccaactga acggtttgat caacagagcc
     2401 tgcatcaact gcgcaagcgg cgttaacaac ttccccttta aagtgaatgg tcccaccatt
     2461 taccgtcgta gtatcggcca gagccgctgc ggaactgagg gacagagccg acagaacaac
     2521 gattgccaga gttttaattt tcatgctgct ttcctttaaa aaaactattt ctaaatcgac
     2581 atgggcagtc gttctgtaca ctttgttttg tcaacgagtt tgactgccaa cactgcacag
     2641 ttttccccca aaagatgaaa catttggggc cattttgact catagaggaa agcatcgcgc
     2701 acaaactttt tcagtttatt tgttggctta atattctatt attatcttta tttatagatt
     2761 tttatattgc atgaggtggt ttttggagag aagaatgagg aagatgcgtc gagccacaga
     2821 aacgttagct ttacatatag cggaggtgat gtgaaattaa tttacaagag aaataattta
     2881 catatcaaat agttagatgc tttttgtcgt tttttaatat ttttatgctt gagaaaaaat
     2941 acgtaactta tttatgatat ggacagtttg gccccaattg tcttgtgttt atttgttttt
     3001 taactttatt atcaataagt taaatcaaac ctcttctctt tttaattttt cgtttatgag
     3061 attatttctt tcccataatc cggcaaaacg agcagcatta ctggcggtat aacgcacagt
     3121 atggcgaata tttcgatgcc cgagataatc ctgaattaaa cgggtatctg caccacgctc
     3181 cgccagttca taaccgcaag catgccttaa catatgagga tgagtctgcg ttacggttcc
     3241 agcttcaata ccggcatcgc gaataatgcg ataggcctgc tggcgagaaa gccgactccc
     3301 gcggcgagaa ataaatatag cgtcggtccg gtcagcgcct ttccagttag cacgttcctg
     3361 gggccagcgt tccacggctt cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc
     3421 gttcttcagt cggcgaatat ttattcgacc ttcattaagg tcaaggtcct gataatgcag
     3481 atcaagcagt tcactaatac gcatcccatg ccgatatgcc aacagaataa gacaataatc
     3541 tctggctccc gttgccccgt aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt
     3601 aagataacga cgtttactca caatagtagt actcctaact gagatatatt tgaatgaata
     3661 cctataggaa acctcaatcg gtcaaaatta gcccaataga taaaaagata accacacacg
     3721 aagttatgtg gctatcagtc aattacatta aacatcatac aaaaataaaa tatcactgat
     3781 gtgacaacat ctattttcat tgatttgatt caatataaca atagattaca gaggattaac
     3841 ttccagttac ttaaaaaata aagaaaaaag tgtggaaaca ggagaattta agtgatgtgt
     3901 aaacttatga atgattttgt ttatttattt tgaagaacat aaatcattag attttgtatg
     3961 ggtatggtta acttaatgtt tattatgata attaagaatc acacttacaa taacatcaat
     4021 taataaatgg ttaatcattt ttgatatatc gtaagaataa tgtagttttt aacaccatcc
     4081 ctggtatctc aactatctct ataaaacagc atgacgctgt cgtcctctgg ctctatccca
     4141 gatgccgtaa aaacgccctg cattgctggc ggtataccag acagtatgac gaatattgcg
     4201 atgcccaaga taatcctaga taagtcgcgt atctattccc atattcgcca aagcaaaacc
     4261 acacgaatag cgtaacatgt gcggatgaat ctccagtgac aacccggcat taccaccaga
     4321 agtcgagata atatggtaaa actgttgccg agaaagcgga ttccccttac gtgataaaaa
     4381 tacccactcg ctctcagcat gcggataaga agtacggata ctcaaccagt tttttaaagc
     4441 ctgaacttct ttgttcaata gcgggtgcgt tgttgaaaag ccttttttta atcgatggat
     4501 atatatacac tttgctttaa gatcaatatc cgaaatcctc aatcgacaaa tttcactcgc
     4561 ccggaaacca tgaataaaac aaagcaaaat cagacaataa ttacgtgttg catgaggccc
     4621 ggtatttgct gctttaagga gtgactcgat ttcactatgg gtaaggaagt tccttttttt
     4681 gttatcagcc ttattcctca tcgttttccc ttataattac agacgcgcac tagctgtgct
     4741 gggttagcaa taatccaaca tttttatcct gattatgttt ataaaagcga acgtttgctt
     4801 aataacttac gtaagtggac cagttcttct gagcgaactt aaatggagta gcgatagtta
     4861 cttataataa ctttgccata caaacgccat gctcttgcat gctatgtacc tttatatatt
     4921 tatcaatctg atacgaatct aataataacc ctcatcatta atgcaactaa tcttatctat
     4981 atatcatgtg atatgttctg taacaagtaa aatcaacata aaaaattcag caaaatttaa
     5041 tatttgttaa actagccgtc tcaaatatga cattattagt taactgcatt tgccacaaaa
     5101 tggctatttc atggaacgca gtaatttctt agtggctatt atcatgctaa ttcttagatc
     5161 cctagcgatt attcctgcct ggttcgtcag cttaggccat gagattgtta cattcctttt
     5221 tatcctggag atatggggga acgcaggcga cgctttttca tctacatccg ttggtaaata
     5281 tagctttggt cactacgact taagccttcc tcaatcacag ctattcgccg ggcgatacgg
     5341 caaacaaact aagtgaaaca tcatcctgaa aacaccaaca tcaacaagcc tctccagatc
     5401 gacatcagga gtgaccagtt acaagccaca aaccaagaaa catatttcat gatgagaatt
     5461 atgctcagcc tgatggcgag taaaccctta ttttcaccac tctcactgag ttagcttgca
     5521 aagctttctt tgtttagatt ctaaataaat tatttataaa acgtatagtt aaaagaaaat
     5581 ctttagtttt tgaagaccac cgcaagtgtt cgtctggctt cacatggcat cttcttctta
     5641 gaaaaagatc gacatatttt gtgacgcgaa ttgcaaatct ggttttgttg tatggattgc
     5701 gtgatttttg atctggtata acaggtataa agatgcacca cgatagtcaa tgagactggg
     5761 catctcgtaa tctatggcaa acatcacttc agttctttca catcgggtga tgaaaatgca
     5821 cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg
     5881 taaaatgggt tgagtaagtg atataactta cgaatattca aatcaattaa aaatcagaag
     5941 agattttata ctcaggtatt taatctggat ctctgtttat ttaaataatg ttaaaagaga
     6001 attttcacag gagaccttat acaaaaaata taaaatacag ttacccgttg ccaaagacac
     6061 tataagcctg gcaaaaaaat attacacaac ataaatgcta attgtttatg cgggctttgt
     6121 attgctttct gtatcctaca aatgagtgaa atttatgaaa aaggctaaaa tactttctgg
     6181 cgtattatta ctgtgctttt cgtccccatt aatttctcag gctgcgacac tggacgtacg
     6241 tggtggatat cgtagtggaa gccacgccta tgagactcga ctcaaagtca gtgagggatg
     6301 gcaaaatgga tggtgggcaa gcatggaaag taatacctgg aataccattc atgataataa
     6361 aaaggaaaat gccgcactca atgatgttca ggttgaagtt aattacgcaa ttaaacttga
     6421 tgatcaatgg acggtgcgcc cgggaatgtt aacgcatttt agcagcaacg gcacacgcta
     6481 cggaccctac gtaaaactgt cctgggacgc gacaaaagat cttaattttg gcattcgcta
     6541 tcgttacgac tggaaagctt accgacaaca agacttatcc ggtgatatgt ctcgtgataa
     6601 cgttcatcgt tgggatggat atgtcactta ccatattaat agtgatttca ccttcgcatg
     6661 gcaaacgacg ctatacagca aacagaatga ttatcgctat gcaaaccaca agaaatgggc
     6721 gacggaaaat gcctttgttc tacaatacca tatgacgccc gatattacgc catacataga
     6781 atatgactac cttgaccgcc agggtgttta caacggcaga gataatttat cggaaaacag
     6841 ttatcgcatt ggtgtgtcat ttaaactgta gtagacagga gacagtcaca atgaataaaa
     6901 ctataatggc gcttgctatc atgatggctt catttgccgc aaacgcgtct gtattaccgg
     6961 aaactcctgt gccatttaaa agtggtaccg gagcaattga taacgacact gtctacattg
     7021 gtttaggtag cgcaggtaca gcatggtaca agctagatac acaggccaaa gataaaaaat
     7081 ggactgcgtt agctgcattc cctggtggac cgagagagca agcaacctct gcatttattg
     7141 atggcaatct gtatgtgttt ggcggcattg gcaaaaacag cgagggattg actcaggtat
     7201 ttaatgacgt acacaaatac aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg
     7261 cgccgatggg catggcgggt catgtgactt ttgtacacaa cggcaaggct tatgttactg
     7321 gcggtgttaa ccagaatatc ttcaatggct attttgaaga tctcaacgag gctggaaaag
     7381 attcaaccgc tatagataaa atcaacgccc actattttga caaaaaagca gaagattatt
     7441 tcttcaataa gtttctgttg tcttttgatc cctcaacaca gcaatggagt tacgctggcg
     7501 aatcgccctg gtacggaacg gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc
     7561 ttattaatgg cgaagccaaa ccaggattgc gaacggatgc cgtatttgaa cttgatttca
     7621 ccggtaataa tttaaaatgg aataagcttg atcccgtctc atcaccagat ggcgtcgctg
     7681 gcggttttgc ggggataagc aatgattctc ttatatttgc tggaggggcc ggattcaaag
     7741 gttcacgaga aaattaccag aacggtaaga actatgcgca tgaaggcctg aaaaaatcat
     7801 atagcactga tattcatctt tggcataacg ggaaatggga taaatcgggt gaattatcgc
     7861 aaggtcgggc ctacggagta tcattgccct ggaataatag tttattaatt attggcggtg
     7921 aaactgcagg cggcaaagcg gtgacggatt cagttttaat ttctgtgaag gataataaag
     7981 tcacagtaca aaactaacgc ttcagggccc cggtaaaggg gccctgctat caatttgtca
     8041 tttgtattac gcgaaaatta tatgaacgca ataatatcac ccgattatta ctatgttctt
     8101 accgttgctg gtcagtctaa tgccatggcg tatggcgaag gactgccatt accggacagg
     8161 gaagatgcgc ctcattccag aattaaacaa ttagcgagat ttgcgcatac gcatcccgga
     8221 ggcccgccat gtcactttaa cgacattatt ccactgactc actgcccaca cgatgttcag
     8281 gatatgcagg gttatcacca tcctctggca acgaatcatc aaacacagta cggcaccgtt
     8341 ggccaggcac tgcatattgc acggaaatta ctgcccttca ttcctgataa tgcagggatt
     8401 cttatcgttc cgtgttgccg tggcggatcg gcttttaccg cgggcagcga agggacatat
     8461 tcagaacggc acggagccag ccatgatgct tgtcgttggg gaacggatac tccgctgtac
     8521 caggatttag tcagcagaac gcgagccgca ctggcaaaaa atccgcagaa caaattcctc
     8581 ggcgtatgct ggatgcaagg cgaatttgac ttaatgacca gtgactacgc gtcacaccct
     8641 caacacttta atcatatggt tgaagccttt cgtagggatc taaaacaata ccattctcag
     8701 cttaataata ttactgacgc gccgtggttt tgcggcgata ccacctggta ctggaaagaa
     8761 aatttcccgc attcgtatga agctatttat ggcaattatc aaaataatgt tttagccaat
     8821 attattttcg tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa
     8881 gacccggacg atttaagcac gggatattac ggttcagcgt accggtcacc ggagaactgg
     8941 acgacggcac tgcgaagcag tcatttcagc acggcagccc gtcgggggat tatttctgac
     9001 agatttgtag aagcaatttt gcagttttgg cgcgaaaggt gagcggggag tttattcatg
     9061 ggggatgtcc ttgttcgttc tcagagtaga caacaaaaaa caaatgagat caaggtaata
     9121 tctcttcact tgcgggctcg aacagcaggc aattgccaat ataataatct ggcacaaggg
     9181 tgaaaaacgc gcaccgcata aaccgttgtt attgcttaca gattagccgg atgccttaat
     9241 aga
//
LOCUS       DAJTRH010000135         9206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTRH010000135 DAJTRH010000000
VERSION     DAJTRH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            422..1117
                     /gene="minC"
                     /locus_tag="OO339_003573"
     CDS             422..1117
                     /gene="minC"
                     /locus_tag="OO339_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5928059.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1141..1953
                     /gene="minD"
                     /locus_tag="OO339_003574"
     CDS             1141..1953
                     /gene="minD"
                     /locus_tag="OO339_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5928060.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1957..2223
                     /gene="minE"
                     /locus_tag="OO339_003575"
     CDS             1957..2223
                     /gene="minE"
                     /locus_tag="OO339_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5928061.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2595..2939)
                     /locus_tag="OO339_003576"
                     /pseudo
     CDS             complement(2595..2939)
                     /locus_tag="OO339_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2974..3093)
                     /locus_tag="OO339_003577"
     CDS             complement(2974..3093)
                     /locus_tag="OO339_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5928062.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3054..3239)
                     /locus_tag="OO339_003578"
     CDS             complement(3054..3239)
                     /locus_tag="OO339_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928063.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3340..3513
                     /locus_tag="OO339_003579"
     CDS             3340..3513
                     /locus_tag="OO339_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928064.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3515..3859
                     /locus_tag="OO339_003580"
     CDS             3515..3859
                     /locus_tag="OO339_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5928065.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3863..4198
                     /locus_tag="OO339_003581"
     CDS             3863..4198
                     /locus_tag="OO339_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5928066.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4255..6576)
                     /locus_tag="OO339_003582"
     CDS             complement(4255..6576)
                     /locus_tag="OO339_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5928067.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNISGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6693..6902)
                     /locus_tag="OO339_003583"
     CDS             complement(6693..6902)
                     /locus_tag="OO339_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928068.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7653..9176)
                     /locus_tag="OO339_003584"
     CDS             complement(7653..9176)
                     /locus_tag="OO339_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5928069.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2575 a         2125 c         2026 g         2480 t
ORIGIN      
        1 gaagtatcag acgtcttctg cggtttctat gatagagcaa ttctgtggcg gatattaaga
       61 taacgggtat ctgttcttta atgatgctgc aaaaaatgga aagttaagaa agcctggcgt
      121 ggtgaaaaaa tacacttgcc tcaatactgg atatttaaac aggtctggag atgctgtcac
      181 gattagggtg cggaaaaagg aagcgtaaat aagaagaatc aaaagtacga cggcaatggg
      241 ttgattgaca agggtatttt ttaagctatg aatcagcgcc atttatcaca gaatagactt
      301 ttactctgaa taaatgggag ggtgacttgc ctcaatataa tccagactat aacatgcctt
      361 atagtcttcg gaacatcatc gcgcgctggc gatgattaat agctaattga gtaaggccag
      421 gatgtcaaac actccaatcg agcttaaagg cagtagcttc actttatctg tggttcatct
      481 gcatgaggca gaacctaagg ttatccatca ggcgctgaaa gacaaaatcg ctcaggcccc
      541 cacattttta aaacatgccc ccgttgtact caacgtcagt gcactggaag acccggtaaa
      601 ctggtcagcg atgcataagg cggtttcggc aaccggtttg cgggttattg gcgtaagtgg
      661 ctgcaaagat gcgcaactta aagccgaaat tgaaaaaatg gggctgccta tcctgacgga
      721 aggaaaagaa aaagcgccac gtccagctcc cgcaccgcag gctccagcgc aaaatacaat
      781 gccggtcaca aaaacgcgtt taatagatac cccggtgcgt tccggtcagc gtatttatgc
      841 tccacaatgt gatctgattg ttacaagcca cgttagcgct ggggccgaat tgattgccga
      901 tgggaacatt catgtctatg gcatgatgcg cggtcgtgcg ctggcagggg caagtggtga
      961 ccgggaaacg caaatatttt gtacgaacct gatggcggaa ttggtgtcca tcgcaggtga
     1021 atactggctg agtgatcaaa tcccagcaga attttatggc aaagcggcgc gactgcagtt
     1081 agtcgaaaac gctttgaccg ttcaaccgtt aaattgatcc ctttttaaca aggaatttct
     1141 atggcacgca ttattgttgt tacttcgggt aaagggggtg ttggtaagac aacctccagc
     1201 gcggccatcg ccactggttt ggcccagaag ggaaagaaaa ctgtcgtgat agattttgat
     1261 atcggcctgc gtaatctcga cctgattatg ggttgcgaac gccgggtcgt ttacgatttc
     1321 gtcaacgtca ttcagggcga tgcaacgcta aatcaggcgt taattaaaga taagcgtact
     1381 gaaaatctct atattcttcc ggcatcgcaa acacgcgata aagatgccct cacccgtgaa
     1441 ggggtcgcca aagttcttga tgatctgaaa gcgatggatt ttgaatttat cgtttgtgac
     1501 tccccggcag ggattgaaac cggtgcgtta atggcactct attttgcaga cgaagccatt
     1561 attaccacca acccggaagt ctcctcagta cgcgactctg accgtatttt aggcattctg
     1621 gcgtcaaaat cacgccgcgc agaaaatggc gaagagccta ttaaagagca cctgctgtta
     1681 acgcgctata acccaggccg cgtaagcaga ggtgacatgc tgagcatgga agatgtgctg
     1741 gagatcctgc gcatcaaact cgtcggcgtg atcccagagg atcaatcagt attgcgcgcc
     1801 tctaaccagg gtgaaccggt cattctcgac attaacgccg atgcgggtaa agcctacgca
     1861 gataccgtag aacgtctgtt gggagaagaa cgtcctttcc gcttcattga agaagagaag
     1921 aaaggcttcc tcaaacgctt gttcggagga taagttatgg cattactcga tttctttctc
     1981 tcgcggaaga agaacacagc caacattgca aaagaacggc tgcagattat tgttgctgaa
     2041 cgccgtcgca gcgatgcaga accgcattat ctgccgcagt tgcgtaaaga tattcttgag
     2101 gtcatttgta aatacgtaca aattgatcct gagatggtaa ccgtacagct tgagcaaaaa
     2161 gatggcgata tttctattct tgagctgaac gtgaccttac cggaagcaga agagctgaaa
     2221 taagcccgct gtaaaaacgc atttatcttc aaggcagagt catttctgcc ttgagttttt
     2281 catccctctc atccacgttg tggtaaagcg gcgagtattc ttgctgatac tcctcattgc
     2341 tatttccacc tcccccttta caccttaagg ctgttcccac gcacagaaca aaatgtttat
     2401 gcctggttga gtaaataacc atgttattag tgtgtatata ctgggagatg ataaccccgc
     2461 aatttcatta ttcattgttc tcccatagga tgaggtttcc cgccgtaact ggcgggcttt
     2521 ttttgcccca gaattttctc tttcaacatc ctgtaaacga aaactgcgcc gaagcgcagt
     2581 ttaatcgttg atggtcagaa cgaccagttc acaccagcca ccgcgttcca cggggactcc
     2641 acaccggcac catggctata ccccaccccg aaatgcccgc ttaacgtact gctgaatgag
     2701 gctttaatac ctgcctggta tattccacgt ctgcccgaca aatcattgac aaaattaccg
     2761 tcactattca ctttcacccg attatcatcg acaaattctt tgcgcacagc cgccttcagg
     2821 cagggttcaa tttccatacc gttccccaga cgcatgttgt aactcagcgt tgcacccagt
     2881 tcacgatata tactgcgggt atcgactgat ttcgattcca tgccattgga taaatgatag
     2941 tagcgtctaa gtgacctaag ctctgctgca acatcagccc gcaggcacca gaccagggga
     3001 ttcatcctga agagacagcg caggtgtatt gtgttcaccg ctcaccaaag acatcatgat
     3061 gaaatgatga tatcccgcat aagaaagagg cattttttac acgcagtgcg ctgaagtgta
     3121 gttggataaa aaatcactct tttcagataa tactgtcgta cgcttatctt tagacaatga
     3181 ggccgtcgga aaaataaaat gactaatgta atcgttgctg atgaatatct tctgctcaca
     3241 taaaaatcac acaataatat tgataaatca gattatctta cctttacaac attgcgttaa
     3301 cagtcaatga cacttcttga ataaaaatat tgtcaatcaa tgagttattc ctcatttaag
     3361 attattctca ttcacgtaaa aaacataatt cctatcatca cagccacgct cattttgaat
     3421 tatctgaata atagcgagcg ttctttagtt aagcagatct taattgaaga cgaaattatt
     3481 gtgtgtgcaa cttacttaat accggacatc taagttgaag aaaaaaatac ttgccttcgg
     3541 gcttatctca gcattgttct gctccacgcc agcgatagcg gatatgaacc gcaccacgaa
     3601 aggtgcattg ctaggcgcag gcgttggttt acttactggc aatggtgtta atggcgtact
     3661 gaaaggtgca gctgttggcg ctggtgttag tgcagtaaca gaaaaaggcc gcgacggtaa
     3721 aaatgcgcgt aaaggtgcaa aagtgggtgc cgccgttggt gctgtcactg gcgttctgac
     3781 cggcaatggc ctcgaagggg cgattaaagg tgccgtgatt ggcggtacgg gtggtgcaat
     3841 tctgggtaaa atgaaataag gaatgatgat gaaaaaattt gctttactgg cgggcctgtt
     3901 tgttttcgca ccaatgacct gggcgcagga ctacaatatt aaaaacggtt tgccgtcaga
     3961 aacctatatt acttgcgcag aagccaacga aatggcaaaa actgacagcg cacaggttgc
     4021 agaaattgtt gcggtaatgg gtaatgccag cgttgccagc cgtgatttaa aaattgagca
     4081 atctccggaa ctgagcgcca aagtggtaga aaaactgaat caggtttgcg ctaaagaccc
     4141 gcaaatgctg ttgatcacgg caattgatga caccatgcgt gctatcggca aaaaataata
     4201 tttcatatgt accgcgtcga tgaatacgac gcggtttttt agctaacggc gggatcagaa
     4261 cttatatttc aggcccacca tcactgcggt gtcattgtag ccattatcac ccagctgcac
     4321 gcccacattc ccccagacgc tggccgctgg attcaggttg ccatttacgc caagtttaat
     4381 ttcccccaaa ttactcaccc catcttgttc gactttcaca ccgttcattg agacagcaaa
     4441 atctttactg ttatggatcc agttcatttc aacaaaaggt tcaaagtcgt caccgtgcgg
     4501 accgctgttt ttctcctgag tacgaataaa ggtgcggaag ccgagtcggg tctggatgtt
     4561 gttatttgcg tcattctcaa tacgtgagcc attggcttcc gtgtgcgcat tatgatcgac
     4621 gcccatccag atagcctgtg cctgtggctg gaagatccac gtatatttcg ccgcttccag
     4681 accaaaggat ttattcaggg caaaaccgta acccgcttcc agcgagacgg ttgcaccttt
     4741 cgcgctatag gattcatagg ctaattcatc ccctttcacg gagtttttaa accagctata
     4801 ttgcgcccag gagtcaatgt atgcgccttt tttactgatg tcatcggcaa accaagtggc
     4861 atacagccct gcgctatagc ctctgacact ccctttcgaa cgataatccg acacgctgga
     4921 atgagttaag ttgtagtcgc gggcataacc agccatcact cccaaacgcc aactgtcact
     4981 atcggtaaaa ccaccggtta acagatcgcc ccctaactgc gagacgtagc gatgcgaggt
     5041 cgttctcaac tgtccgttgc tgtcacgcca ggcattatgc ccgccaattt gacgtagcca
     5101 aagtcggctt gaacgctcct gttcagttac aggatcgatg tagcgcgttt cacccgcacg
     5161 gtcgttcaga cgcataacaa aaagagtgtt agcggctgcc aggttggcga cgtaacttcc
     5221 agcttcgggg cgcaggacgg gggtaggcgc aacagtaggc gctgaacttc ctccggagtt
     5281 tgtttcaccg ccgctcgttg cctggaagtt agtcagatac cagtttttat tggtcgcttc
     5341 ggtattacct cgggttaatg aatattcgta ggcaccggcg aaaatcctcg aatccttaat
     5401 aaattcccca tttgattccc cctcaacgct gataatctct atcccattta acgtgtaagc
     5461 tccgctgcct cctgcattgg taactttaac tcgggttgtt ccagagatat ttccattaat
     5521 aacgacttta tccgtagctg aattatcatc atccaattca gttctgagat gcaatacgcc
     5581 attattacca acgtaatttt cagttattgt taatcttgtt ggttcgacat cccgtccatc
     5641 tgcccgggaa atatagaccg tactattatc aaccgtgagg ttgctcaccg ttgagtcagc
     5701 tttaatatcc caggtactat tatctgaaag cgacagatag gtatgagtgt tatcatctgt
     5761 tgaaatatta gctgaacccg ttatttttga attagttgca tcgacggtca aatgaccttt
     5821 aatattattg ataaaaacat ctgcagccga tgatacctca ctatccgtaa tggtcacgtt
     5881 actcgtctca tctttatggt agaggatttc atttgctgac cgtagtgagc taccagataa
     5941 aatatctacc gttgcttcct gaccatttat ggaagactcc agcacaattc cagacccctg
     6001 atgcgcgact gcagatgtat ttttcagcgt caccgtgccg ccattataaa ttttagcaat
     6061 cgcggcgtca ggacccgttg tataaatttc tccctggtta aatgaaactg tcccctggcg
     6121 ggcagcgatg gcatgagcat tgtccccatg cgtggtgata tcaacatcgg ttaatgtaac
     6181 gctggcattg gtataatcaa gcgtgtcacc agccttgacg acagtgccat tagcgtacgc
     6241 ggcataactt tctgttccgt gggttgtgat ctcccctttt gccatatcca gttttgctat
     6301 ggcattattt tcattacttc ctgtatcggc agtactttct tcatcaagag aagatacaca
     6361 cgcacacgcg cttccgggag atgcacacgc aagaacgccg actgcgtgat tacccgcagt
     6421 aacaataaat gtatcatctc cgtcagaaaa agagggggta accgaaccgt cagagttgac
     6481 tcttattatg gtgtcagtat tcaggtttcc actcgtttga cttagcaaac caattgagta
     6541 atcccccgtt gtgacaatcg tgtttttacc ctgcaaaata acagcaccgc caaattcact
     6601 tacgattgcg ctagctgtta atgctgggct tagaggacta ttgtcagaag gatataaacc
     6661 gcccgttgtt acaatgacgc cattgttaat ggttaactta ctaccctctc ctgcaacgga
     6721 tacccgtaac ttccattacc cgttgttata actagcgcat tatcaatatc ttttactttt
     6781 ccatctttta ttgccacagc atgtttatat tcatcgtgct gaataaactg ttcagcatat
     6841 gcattaccca ccgccagagc aataagtccc aaactaaacc cggggagttt tttcagtttc
     6901 atagattaac tatccattca ttggattaac aaacattcct ggtcaaataa cctatgagct
     6961 cagattacca aaacggaggg taaagacaaa aacaaaagca caaaaaattt aaatagataa
     7021 acagcgacaa tatataaatg ataaataagt cggcattatc atttttcacc ttattttcat
     7081 gacattgatc actttggggt gactattttg tttaacaaat aactcttcga catcaaaatt
     7141 attaatagtt gccatggtaa ttatttcggt gatatataaa aacaactgtg ataacaccaa
     7201 ggttgacaac tatttgcgca ataattcctt tccttcaatg atccaggcgt tcactgccag
     7261 caacccattc agccgcatac tgacagtgag aaactgaaag attattgctg atccctgtgg
     7321 ctaacgagtc tttctcttaa atcgattaac cagattgatc gccagccaaa caagggcatt
     7381 aagcaaattt atcgtcacaa gcacttctcc cagcccggta ctgggttttg caataaggat
     7441 gaccaggagg gtgatcagca taaatgcaat ggaaaagatg atatagagag tgaaataatc
     7501 cagattatta ctattattca tgctactaat ggcaatcctg tcaaaacttt caaacaatta
     7561 tagcaaacaa aataatgaac aatagtaaca agaggcaacg agctaattag ctgtctataa
     7621 ttacaccgca ctatggcctt actgatcaat tttcactcaa ccacaacctt caccttcggt
     7681 ttagaaagga taattttgac caggtcgatg tatgtaacag gtttatagaa ataataaccc
     7741 tgctgaaatg tgatattatt ttggttcaga tagccaagtt gttctttcgt ttcgacacct
     7801 tcagcgacga tactgatcga aagtttacgc gccagatcca atacacaatc cagaattcgg
     7861 gttgattctg ggtctgcgtt tacgcggcca acgaaactgt gatcaatttt gatataatca
     7921 atatgcaggt catgaagata agaaagccct gagtagccag taccgaaatc atccagcgcg
     7981 atgacaaaac cattttcatg cagaatgttc aaccgctgaa ccagactttc atcaacattc
     8041 aatggctcac gctcggtgac ttccagaaca aggtttaaat cgcggcgggt aaaactgtca
     8101 cggaaattta aacactcgtc gacaaacgtc ggcgaaataa tatgcgaagc gctaaaatta
     8161 attccaatat gaaaaccttc cggcagttta ctcgcgatag cgttcatctg tctggcaacc
     8221 tgattcatca ggctttgtgt aagcggaacg attaatccgg atttttcagc aagtggaata
     8281 aatgccgcgg gtgaaatata tccaccgtga ggttgtttcc agcgggctaa cacctcaact
     8341 ccccgcaatg tcccttcccg accatttacc acaggttggt aaaaagggac gatctcccca
     8401 ttatttattg cccgtcgcag gatctcttca ggcgttgcgc ttttattgaa atatctatca
     8461 agcaggaagg cagcggcgct ggcgattaac agaataaata ttaaaatccc aaagccttta
     8521 tcgataagtc tgtagaagct aaagagtggc ggcgggttaa agataatagt gaaatgatat
     8581 tttgtggagt gaaccttctc ggtataggga taatgtccat taagcgtcat cacatcagcc
     8641 gttggtccaa taatgtcatc cgccacgcgt aatacatacc ttaccccttt caagggtaca
     8701 ttaagcgccc cgcgaatatg ttgatcactt atcgtaacca aaatgcgcgt atctgcaaat
     8761 tggctctgat agagcaggat aggtaatcta ttaacggtgt cgattgccgg agccaacagt
     8821 aaattactgt ccgggacaac aggaatacga gacagcagga cccgattccc aggcagggat
     8881 gtacaccaga ctattccctg tttgagaatg atgattgtgc gcaggtgagg tttcagagct
     8941 gcttcagtgc caagctgata ttgctcctcg aggtcgcatt ccttcccggc aatgttaacc
     9001 gctgtttgag tcgcctgtga cgcttcttca agtatatgat tgatattgtt ggcggcatat
     9061 cttgctcccg ccaggtactc tgcttttgcg gaataccaga gctggatgtt caaaatagcg
     9121 acaccggtaa taaacaagca tatcgcaacg aggatgggta tgagtgtatt gcgcatttgc
     9181 ttgtcccggc caaagggttt aataca
//
LOCUS       DAJTRH010000136         9019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTRH010000136 DAJTRH010000000
VERSION     DAJTRH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO339_003585"
     CDS             complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO339_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5928070.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO339_003586"
     CDS             complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO339_003586"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5928071.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO339_003587"
     CDS             complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO339_003587"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5928072.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO339_003588"
     CDS             complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO339_003588"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5928073.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4456..5580
                     /gene="dprA"
                     /locus_tag="OO339_003589"
     CDS             4456..5580
                     /gene="dprA"
                     /locus_tag="OO339_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5928074.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5552..6025
                     /locus_tag="OO339_003590"
     CDS             5552..6025
                     /locus_tag="OO339_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5928075.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6054..6596
                     /locus_tag="OO339_003591"
     CDS             6054..6596
                     /locus_tag="OO339_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5928076.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6601..7173
                     /gene="tsaC"
                     /locus_tag="OO339_003592"
     CDS             6601..7173
                     /gene="tsaC"
                     /locus_tag="OO339_003592"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5928077.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7178..7996
                     /gene="aroE"
                     /locus_tag="OO339_003593"
     CDS             7178..7996
                     /gene="aroE"
                     /locus_tag="OO339_003593"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5928078.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7993..8250
                     /locus_tag="OO339_003594"
     CDS             7993..8250
                     /locus_tag="OO339_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5928079.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8226..8780)
                     /locus_tag="OO339_003595"
     CDS             complement(8226..8780)
                     /locus_tag="OO339_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5928080.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2254 a         2190 c         2251 g         2324 t
ORIGIN      
        1 ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa agggaaataa
       61 taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg gaagagtctt
      121 tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc gccttgctca
      181 atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc tccaataatc
      241 gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga ggtgctttca
      301 tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga ggaaacacca
      361 acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa
      421 attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg ctggatcaat
      481 accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat attggcctcg
      541 tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc ggccagcagt
      601 tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc cgccagttcg
      661 gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc cagacgacgc
      721 gccagccctg caccgatatt accgccacca accagcatga tccgtttata cggtttttcc
      781 agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat aaagaacact
      841 tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc gtggcggaaa
      901 attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga aagtgcatta
      961 ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt accctcagcg
     1021 aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt atcgataacc
     1081 aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa tagcttatcg
     1141 gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt gttgaaaagt
     1201 gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac agcaaccagc
     1261 atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc
     1321 acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt ggtatcgaca
     1381 acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc aacctggccg
     1441 gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt tttgattagc
     1501 ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt acccggttgc
     1561 tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa ggctttaatc
     1621 tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt tccaccggat
     1681 tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag ttccgggata
     1741 tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc cgttgctgaa
     1801 caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg ggaagggtaa
     1861 cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt gtcgtaaatg
     1921 cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg tgccacctca
     1981 aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg ttcaccgttt
     2041 tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt aacccatccg
     2101 tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac agcatcaggg
     2161 taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag ccagctgtcg
     2221 cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt attggcttcg
     2281 gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa ccaggaagga
     2341 tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg ccgacgctgg
     2401 aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat tgcgatagcg
     2461 ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag cagttgatac
     2521 aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg acgggccatt
     2581 aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa gcacaactct
     2641 tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag aatgttgctt
     2701 aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct acgtaaatta
     2761 cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag agtggactat
     2821 cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag gtcttgcgcg
     2881 ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc atcaccagtc
     2941 gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc gttggttgcc
     3001 gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc cagccagctc
     3061 attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga aagtgaccag
     3121 tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt ttcgtcctga
     3181 acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag cccttgtggg
     3241 ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt aatcgggcag
     3301 gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat aatggttaca
     3361 ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc gcgccagcgt
     3421 ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag cacagcttta
     3481 ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc ggcgaccagt
     3541 tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc ttcttcctca
     3601 gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg tcggtctggc
     3661 tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag atgacgcgct
     3721 gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt agttcttatc
     3781 cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt cgttgttgtt
     3841 tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc tcatgctgaa
     3901 tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg cggtcaaggg
     3961 cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg atcgacaggc
     4021 aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg attaacacca
     4081 gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg atatcaacct
     4141 gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata tcatcgacga
     4201 tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga agccgctcgt
     4261 ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt caggagttag
     4321 aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc acttgattga
     4381 cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag gcttgcttat
     4441 ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag catcagcagc
     4501 ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc gcatattgat
     4561 gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt tctttcattt
     4621 ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca tcatttaatc
     4681 cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta ccccggcgca
     4741 ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt agtggggagt
     4801 cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac tctggcgacg
     4861 tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt gcataaagcg
     4921 gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa taccattcat
     4981 ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc tctcgtctcg
     5041 gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa tcgcattatc
     5101 agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg
     5161 acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg tccaataggg
     5221 aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct tgtgacggaa
     5281 ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga cgcccctgaa
     5341 aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga gctcctggct
     5401 aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg
     5461 ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc agctgtaccc
     5521 ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt atttgtttga
     5581 aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg aacaggatct
     5641 taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc tggaaaaact
     5701 tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc ctctctccat
     5761 gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg ggtttctgct
     5821 tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga tagagcgagt
     5881 gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga tcctgatggt
     5941 gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat tactctttga
     6001 agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt gttatggcaa
     6061 aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg gctgaactgg
     6121 ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg gcgtgtgact
     6181 acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg gaggggcagg
     6241 tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt atgtttattg
     6301 gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg gacgaaacag
     6361 caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc cgttatggca
     6421 aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa
     6481 tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa accgcgcagg
     6541 gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg gaataataac
     6601 gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct caatgaaaaa
     6661 cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc tgatagcgaa
     6721 acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg gctgatttta
     6781 atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt gactgacgtg
     6841 cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt tcccgcgcct
     6901 gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg agtcaccgac
     6961 catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc taccagtgcc
     7021 aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg
     7081 gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga aatccgcgat
     7141 gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg ctgtttttgg
     7201 taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc agcaactgaa
     7261 tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca acacactgaa
     7321 cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc
     7381 ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag ttaataccct
     7441 caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag gcttgttaag
     7501 cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta tcggcgctgg
     7561 tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg tgacaatcac
     7621 taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca ctggcagtat
     7681 tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta ttaatgcaac
     7741 atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc atccaggcat
     7801 ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat ggtgtgagca
     7861 gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg cggctcatgc
     7921 ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc aattgcagga
     7981 ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg ggacgagaat
     8041 aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg cgcgatctct
     8101 ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt agcgagtttt
     8161 cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga acaaagtgaa
     8221 gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca tttcacgtaa
     8281 ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg gatctgtttg
     8341 acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg gaccagacta
     8401 cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat tgagcccatc
     8461 ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg accaacagtg
     8521 acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt atgagtgaca
     8581 tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata atgtacatct
     8641 ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac gtcaccaatc
     8701 acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag atcgcggtat
     8761 gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat gactttgggg
     8821 gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgatt
     8881 gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat acttgtgcaa
     8941 aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca cttcacagga
     9001 tggtcggaac aacgaagag
//
LOCUS       DAJTRH010000137         9012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTRH010000137 DAJTRH010000000
VERSION     DAJTRH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..918
                     /locus_tag="OO339_003596"
     CDS             <1..918
                     /locus_tag="OO339_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5928081.1"
                     /translation="IGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPPA
                     LVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQL
                     QFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPF
                     LAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAF
                     ISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTH
                     SFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1170..2087
                     /gene="oxyR"
                     /locus_tag="OO339_003597"
     CDS             1170..2087
                     /gene="oxyR"
                     /locus_tag="OO339_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5928082.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2070..3470)
                     /gene="sthA"
                     /locus_tag="OO339_003598"
     CDS             complement(2070..3470)
                     /gene="sthA"
                     /locus_tag="OO339_003598"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5928083.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3791..4441
                     /gene="fabR"
                     /locus_tag="OO339_003599"
     CDS             3791..4441
                     /gene="fabR"
                     /locus_tag="OO339_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5928084.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4441..4800
                     /locus_tag="OO339_003600"
     CDS             4441..4800
                     /locus_tag="OO339_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5928085.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4840..5940)
                     /gene="trmA"
                     /locus_tag="OO339_003601"
     CDS             complement(4840..5940)
                     /gene="trmA"
                     /locus_tag="OO339_003601"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5928086.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6054..6244
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6309..8153
                     /gene="btuB"
                     /locus_tag="OO339_003602"
     CDS             6309..8153
                     /gene="btuB"
                     /locus_tag="OO339_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5928087.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8098..8955
                     /gene="murI"
                     /locus_tag="OO339_003603"
     CDS             8098..8955
                     /gene="murI"
                     /locus_tag="OO339_003603"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5928088.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2047 a         2177 c         2436 g         2352 t
ORIGIN      
        1 attggcgaag cgccatttat gcctgcggga gtaaagtcga tcaccgactg gtatgcacaa
       61 aaagaacgcg gcacggcgct ggggattttt aactcatcta ccgttatcgg tcaggctatc
      121 gcgcctcctg ctcttgtatt gatgcagctg gcatggggct ggcggacgat gttcgttatc
      181 atcggcgtgg cagggattct ggttgggatc tgttggtacg cgtggtatcg caaccgggcg
      241 cagtttgtcc tgactgacga agaacggacg tatctctccg cgtcggttaa accgcgtcca
      301 cagctgcaat ttagcgagtg gctggcgctg tttaagcatc ggacaacctg ggggatgatt
      361 ttaggtttct ctggcgtcaa ttataccggt tggctttaca ttgcgtggtt acccggttat
      421 ttgcaagccg aacaaggttt cagtctggcg aaaaccggtt gggtagcggc gattcctttc
      481 ctcgcggcgg cggtcgggat gtgggttaac ggtattgtgg tcgatcggct ggcgaaaaaa
      541 ggctacgacc tggcgaagac gcgtaaaacg gctattgtct gcggtttgat gatgtcggca
      601 ttaggcacgt tgctggtcgt gcaatcttcc tcgccagctc aggcggtagc gtttatctca
      661 atggcgctgt tctgcgtgca tttcgcggga acgtctgcat ggggactggt gcaggtgatg
      721 gtgtcagaaa caaaagtggc ttccatcgcc gggattcaaa actttggcag ttttgttttt
      781 gcttcctttg ctccgatcgt aaccggttgg gtagtggata ccacacactc gtttaatctg
      841 gcgctggtta tcgcggcctg tgtgacgttc accggggcgc tgtgttactt ctttattgtc
      901 aaagatcgca ttgagtaatc gttactatca ggctctcttg ctgtgggcct gtagaataaa
      961 aaaaagcgga tcctggagat ccgcaaaagt tcacgttggc tttagttatt cgagttgaga
     1021 aactctcgaa acgggcagtg acttcaaggg ttaaaagagg tgccgctccg tttctgtgag
     1081 caattatcag tcagaatgct tgatagggat aatcgttcat tgctattcta cctatcgcca
     1141 tgaactatcg tggcgatgga ggatggataa tgaatattcg tgatcttgag tacctggtgg
     1201 cattggctga acaccgccat tttcggcgtg cggcagattc ctgccacgtt agccagccga
     1261 cgcttagcgg ccaaattcgt aagctggaag atgagctggg cgtgatgttg ctggagcgga
     1321 ccagccgtaa agtgttgttc acccaggcgg gaatgctgct ggtggatcag gcgcgtaccg
     1381 tgctgcgtga ggtgaaagtc cttaaagaga tggcaagcca gcagggtgag acgatgtccg
     1441 gaccgctgca tattggtttg attcctacgg ttggaccata cctgctaccg catattatcc
     1501 cgatgctgca ccagacattt ccaaagctgg aaatgtatct gcatgaagca cagacccacc
     1561 agttactggc gcaactggac agcggcaaac tcgattgcgt gatcctcgcg ctggtgaaag
     1621 agagcgaagc attcattgaa gtgccgttgt ttgatgagcc aatgttgctg gctatctatg
     1681 aagatcaccc gtgggcgaac cgcgaatgcg tactgatggc cgatctggca ggggaaaaac
     1741 tgctgatgct ggaagatggt cactgtttgc gcgatcaggc aatgggtttc tgctttgaag
     1801 ccggggcgga tgaagataca cacttccgcg cgaccagcct ggaaactctg cgcaacatgg
     1861 tggcggcagg tagcgggatc actttattgc cagcgctggc tgtgccgccg gagcgcaaac
     1921 gcgatggggt tgtttatctg ccatgcatta agccggaacc atgccgcact attggcctgg
     1981 tttatcgtcc tggctcaccg ctgcgcagcc gctatgagca gctggcagag gccatccgcg
     2041 caagaatgga tggccatttc gataaagtgt taaaacaggc ggtttaaacc gtttaacgca
     2101 gctacccgat aggcttccgc catcgtcggg tagttaaagg tggtgttgac gaagtactca
     2161 atagtgttgc cgccaccttt ctgttccata atcgcctgac cgatatgaat aatttcggca
     2221 gcgcgctcgc caaagcagtg aatacccaga atctcttttg tttcccgatg gaacaaaatt
     2281 ttcagcgtgc ccacgttcat gccgacgatt tgtgcacgtg ccagatgttt gaactgggcg
     2341 cggcccactt catacggcac tttcatcgcg gtcagctgct gttcggtttt gcccacagag
     2401 ctgatttccg ggatggtgta aatgccggta gggatatctt caatcagatg tgcggtggct
     2461 tcgcctttta ccagcgcctg cgcggcaatg cgcccctggt cataggccgc tgatgccagg
     2521 ctcggataac caatcacgtc gcccaccgcg taaacgtgcg gctgtgcggt ctgatacatg
     2581 ctgttgacct tcagctgtcc gcgactgtca gtttccagcc caatgttctg taacgccagc
     2641 gaatcggtat taccggtacg accgttggca tagagcaggc agtcagcttt cagtttttta
     2701 cccgacttca gatgcatgat cacaccgtcg tcacagcttt cgatcttctc gtactcttcg
     2761 ttgtgacgaa tcactacgcc actgttccag aagtggtagg agagagaatc tgacatctct
     2821 tgatcgagaa atgccagcag gcgatcgcgg gtgttgatca gatccacttt tacatccata
     2881 ccgcggaaga tcgacgcata ttcacagccg atcactccag caccatagat aagtacatgg
     2941 cgcggttcgt ggtgcatgct aagaattgag tcgctgtcgt aaatgcgtgg atgggtgaaa
     3001 tcaacatctg ttggatgata tggacgagag ccgcaggcaa taacaaattt ttcagcggtt
     3061 agtgtttcaa cgctgccgtc cgggcaatcc agcgccaacg tatgctcgtc aacaaagcga
     3121 gcgttcccct gcaatatttc acagtgatta cgttcgtaaa atccctgacg catgcgcgtt
     3181 tgttgattaa tcacgttatc ggcatggtta aggatatcgg caaaagaaga gcggagcagt
     3241 cgggaatggt cgctgtatag tgggttttga ttgaattcta taatgcggct gacggcgtga
     3301 cggagagctt tcgacgggat ggtgccccag tgggtgcaac cgccgccaac attttgataa
     3361 cgctcgataa ctgcgacgcg cgcaccttgc ttaaccaggc ccattgcagc gccttcgccg
     3421 ccggggccgg aacctattac tatggcatcg taatcgtagg aatgtggcat ggtagggctt
     3481 acctgttctt atacataaaa gcaacagaat ggtaacattc tatcgcgggt aagccaattg
     3541 atccccgtca tttatctggc tatatcctga gcggcctttg ctttgtctgt ttcttacttt
     3601 tgccctgacg ttttattgga tttttatcga cgatactctc cgtttaagcg gcaggtttcc
     3661 gctgtacgta aaagaaccgg ccagtaaata tgttttattg cgttaccgtt cattcacaat
     3721 actggagcaa tccagtatgt tcattctctg gtatagtgcc agcagtactt ttggcaagga
     3781 ttcagacatc gtgatgggcg taagagcgca acaaaaagaa aaaacccgcc gttcgctggt
     3841 ggaagccgca tttagccaat taagtgctga acgcagcttc gccagcctga gtttgcgtga
     3901 agtggcgcgt gaagcgggca ttgctcccac ctctttttat cggcatttcc gcgacgtaga
     3961 cgaactgggt ctgaccatgg ttgatgagag cggtttaatg ctacgccaac tcatgcgcca
     4021 ggcgcgtcag cgtatcgcca aaggcgggag tgtgatccgt acctcggtct ccacatttat
     4081 ggagttcatc ggtaataatc ctaacgcctt ccggttatta ttgcgggaac gctccggcac
     4141 ctccgctgcg tttcgtgccg ccgttgcgcg tgaaattcag cacttcattg cggaacttgc
     4201 ggactatctg gaactcgaaa accatatgcc gcgtgcgttt actgaagcgc aagccgaagc
     4261 aatggtgaca attgtcttca gtgcgggtgc cgaggcgttg gacgtcggcg tcgaacaacg
     4321 tcggcaatta gaagagcgac tggtactgca actgcgaatg atttcgaaag gggcttatta
     4381 ctggtatcgc cgtgaacaag agaaaaccgc aattattccg ggaaatgtga aggacgagta
     4441 atgaaacaag caaatcaaga tagaggtacg ctgctgctgg cgttggttgc tggtttatcg
     4501 attaatggta ctttcgcagc gctgtttagc tccattgtgc cattttctgt attcccgatt
     4561 atttccctgg tgctgacggt ttactgcctg catcaacgtt atcttaatcg caccatgtcg
     4621 gtaggcttgc cgggtctggc agctgcctgt tttattctcg gcgtactgct gtacagcacg
     4681 gtagttcgtg cggaatatcc ggatatcggc tctaacttct tcccggcagt actctccgtc
     4741 attatggtgt tctggattgg cgcgaagatg cgtaaccgta agcaggaagt tgctgagtaa
     4801 tcggtatcat gccggatgct ggcgcatccg gcatgggttt tacttcgcgg tcagtaatac
     4861 gccgcactcc atatggtgcg tgtaggggaa ctgatcaaac agagccagac gttcgacctt
     4921 gtgcgtctgg cttaatgttt ccagattttt gcataacgtt tccggattgc aggagatata
     4981 cagaatacgt ggatacgcct gcaccatttt ctcggtttca ctgtccagac cgctgcgcgg
     5041 tgggtcgaca aaaatggttt cgaactgata gctctttaag tcgatccctt gcaggcggtt
     5101 aaactcgcgc acaccattca tcgcctgagt aaattcttct gccgccatac gaataatttg
     5161 tacgttatca atatggttag cggcaatgtt gtattgcgcg gcagcgactg acggtttggc
     5221 gatttcggtg gctaataccc gatcaaaatt gcgcgccagc gctaatgaaa agttaccgtt
     5281 gccgcagtac agctccagta aatcgccttt tgagcctttg gttacgtcca gcgcccattc
     5341 cagcatctga atattcatcg ccgcgttcgg ctgggtaaag ctgttttcta cctgacgata
     5401 gatcatctct ttccctgcga ccggcagacg ctcgtcgatg taatcttgat ccagcgcgat
     5461 tttggttttc gttgcccgac caatcagatg cacattcaga ttctgcgcgc gcagtgcatc
     5521 gcgcagtgct tccgcttcct gacgccactc atcatccagc tttttatggt acagcaggga
     5581 aaccaccgcc tgattactca gcgtggtgag gtaatcaatc tggaacaact tgtggcgcag
     5641 aacgggatta ttacgcacac ccgcaatcat cgccgtcatc aactggttga tgagttcact
     5701 ggcggcgggg aagctatcca cgcggatgcg gcttttggtt tgttgatcga aaatgatgtg
     5761 atacaggtca tcgccatcgt gccagatgcg gaactccgcg cgcatccggt aatgactgac
     5821 cggcgagcga aacacttccg gaaccaggtc agaaaacggt gccatcatac tttgcaaacg
     5881 taccactttt tcggctaact gcgcttcata ctgttctgtt ggaaggtgtt cgggggtcat
     5941 gatgtatcct gaaaaattaa agtacgcggc gattgtaggg attgctcatc agatgtccag
     6001 atcttgatga attcctattt gtgagctacg tctggacagt aacttgttac aacctgtagc
     6061 atccacttgc cggtcctgtg agttaatagg gaatccagtg cgaatctaga gctgacgcgc
     6121 agcggtaagg aaaggtgcga tgattgcgtt atgcggacac tgccatccgg tgggaagtca
     6181 tcatctctta gtatcttaga tacccctcca agcccgaaga cctgccggcc aacgtcgcat
     6241 ctggttctca tcatcgcgta atattgatga aacctgcggc atccttcttc tattgtggat
     6301 gctttacaat gattaaaaaa gcttcgctgc tgacggcgtg ttccgtcacg gcattttccg
     6361 cttgggcaca ggataccagc ccggatactc tcgtcgttac tgctatccgt tttgaacagc
     6421 cgcgcagcac tgtgcttgca ccaaccaccg ttgtgacccg tcaggatatc gaccgctggc
     6481 agtcgacctc ggtcaatgat gtgctgcgcc gtcttccggg cgtcgatatc acccaaaacg
     6541 gcggttcagg tcagctctca tctattttta ttcgcggtac aaatgccagt catgtgctgg
     6601 tgttaattga tggcgtccgc ctgaatctgg cgggggtgag tggttctgcc gaccttagcc
     6661 agttccctat tgcgcttgtc cagcgtattg aatatatccg tggaccgcgc tccgctgttt
     6721 atggttccga tgcaataggc ggggtggtga atatcatcac gacgcgcgat gaacccggaa
     6781 cggaaatttc agcagggtgg ggaagcaata gttatcagaa ctatgatgtc tctacgcagc
     6841 aacaactggg ggataaaaca cgggtaacgc tgttgggcga ttatgcccat actcatggtt
     6901 atgatgttgt tgcctatggt aataccggaa cgcaagcgca gacagataac gatggttttt
     6961 taagtaaaac gctttatggg gcgctggagc ataactttac tgatgcctgg agcggctttg
     7021 ttcgcggcta tggctatgat aaccgtacca attatgacgc gtattattct cccggttcac
     7081 cgttgctcga tacccgtaaa ctctatagcc aaagttggga cgccgggctg cgctataacg
     7141 gcgaactgat taaatcacaa ctcattacca gctatagcca tagcaaagat tacaactacg
     7201 atccccatta tggtcgttat gattcgtcgg cgacgctcga tgagatgaag caatacaccg
     7261 tccagtgggc aaacaatgtc atcgttggtc acggtagtat tggtgcgggt gtcgactggc
     7321 agaaacagac tacgacgccg ggtacaggtt atgttgagga tggatatgat caacgtaata
     7381 ccggcatcta tctgaccggg ctgcaacaag tcggcgattt tacctttgaa ggcgcagcac
     7441 gcagcgacga taactcacag tttggtcgtc atggaacctg gcaaaccagc gccggttggg
     7501 aattcatcga aggttatcgc ttcattgctt cctacgggac atcttataag gcactaaatc
     7561 tggggcaact gtatggcttc tacggaaatc cgaatctgga cccggagaaa agcaaacagt
     7621 gggaaggcgc gtttgaaggc ttaaccgctg gggtgaactg gcgtatttcc ggatatcgta
     7681 acgatgtcag tgacttgatc gattatgatg atcacaccct gaaatattac aacgaaggga
     7741 aagcgcggat taagggtgtc gaggcgacag ccaattttga taccggacca ctgacgcata
     7801 ctgtgagtta tgattatgtc gatgcgcgca atgcgattac cgacacgccg ttgttacgcc
     7861 gtgctaaaca gcaggtgaaa taccagctcg actggcagtt gtatgacttc gactggggta
     7921 ttacttatca gtatttaggc actcgctatg ataaggatta ctcatcttat ccttatcaaa
     7981 ccgttaaaat gggcggtgtg agcttgtggg atcttgcggt tgcgtatccg gtcacctctc
     8041 acctgacagt tcgtggtaaa atagccaacc tgttcgacaa agattatgag acagtctatg
     8101 gctaccaaac tgcaggacgg gaatacacct tgtctggcag ctacaccttc tgaaccacgt
     8161 cccaccgtgc tggtgtttga ctccggcgtc ggtgggttgt cggtctatga cgagatccgg
     8221 catctcttac cggatctcca ttacatttat gctttcgata acgtcgcttt cccgtatggc
     8281 gaaaaaagcg aagcgtttat tgttgagcga gtggtggcaa ttgtcaccgc ggtgcaagaa
     8341 cgttatcccc ttgcgctggc tgtggtcgct tgcaacaccg ccagtaccgt ttcacttcct
     8401 gcattacgcg aaaagttcga cttcccggtt gttggtgtcg tgccggcgat taaacctgct
     8461 gcacgtctga cggcaaatgg cattgtcgga ttactggcaa cccgcggaac agttaaacgt
     8521 tcttatactc atgagctgat cgcgcgtttc gctaatgaat gccagataga aatgctgggc
     8581 tcggcagaga tggttgagtt ggctgaagcg aagctgcatg gcgaagatgt ttctctggat
     8641 gcactaaaac gtatcctacg cccgtggtta agaatgaaag agccgccaga taccgttgta
     8701 ttgggttgca cccatttccc tctactacaa gaagaactgt tacaagtgct gccagaggga
     8761 acccggctgg tggattctgg cgcagcgatt gctcgccgaa cggcctggtt gttagaacat
     8821 gaagccccgg atgcaaaatc tgccgatgcg aatattgcct tttgtatggc aatgacgcca
     8881 gaagctgaac aattattgcc cgttttacag cgttacggct tcgaaacgct cgaaaaactg
     8941 gcagttttag gctgatttgg ttgaatgttg cgcggtcgga aaattatttt aaatttcctc
     9001 ttgtcaggcc gg
//
LOCUS       DAJTRH010000138         8762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTRH010000138 DAJTRH010000000
VERSION     DAJTRH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            5..436
                     /locus_tag="OO339_003604"
     CDS             5..436
                     /locus_tag="OO339_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5928089.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO339_003605"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO339_003605"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5928090.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO339_003606"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO339_003606"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5928091.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO339_003607"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO339_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5928092.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="OO339_003608"
     CDS             complement(4438..5145)
                     /locus_tag="OO339_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5928093.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO339_003609"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO339_003609"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5928094.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="OO339_003610"
     CDS             complement(6003..6206)
                     /locus_tag="OO339_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5928095.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="OO339_003611"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="OO339_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8762
                     /locus_tag="OO339_003612"
     CDS             7870..>8762
                     /locus_tag="OO339_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5928096.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT         2034 a         2388 c         2251 g         2089 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtaa
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgggaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacctgaa
     8761 ca
//
LOCUS       DAJTRH010000139         8629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTRH010000139 DAJTRH010000000
VERSION     DAJTRH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OO339_003613"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OO339_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5928097.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3166)
                     /gene="hemX"
                     /locus_tag="OO339_003614"
     CDS             complement(1967..3166)
                     /gene="hemX"
                     /locus_tag="OO339_003614"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5928098.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(3188..3928)
                     /gene="hemD"
                     /locus_tag="OO339_003615"
     CDS             complement(3188..3928)
                     /gene="hemD"
                     /locus_tag="OO339_003615"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5928099.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3925..4866)
                     /gene="hemC"
                     /locus_tag="OO339_003616"
     CDS             complement(3925..4866)
                     /gene="hemC"
                     /locus_tag="OO339_003616"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5928100.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5253..7799
                     /gene="cyaA"
                     /locus_tag="OO339_003617"
     CDS             5253..7799
                     /gene="cyaA"
                     /locus_tag="OO339_003617"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5928101.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7839..8159)
                     /gene="cyaY"
                     /locus_tag="OO339_003618"
     CDS             complement(7839..8159)
                     /gene="cyaY"
                     /locus_tag="OO339_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5928102.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8207..>8629)
                     /locus_tag="OO339_003619"
     CDS             complement(8207..>8629)
                     /locus_tag="OO339_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5928103.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1964 a         2232 c         2218 g         2215 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatccacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtt
     2041 tagcttccgt tgtccccgct gccggttgtg ccagcaggtt acgcacgcga gtctgcatca
     2101 gtttttccag catcgcctgg ctttgcaggg tttccggaag atccatcgag atattttgct
     2161 ggcttaactg gtccacctcg tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt
     2221 aagcacgtac ccaggtggag acgttctcca gcgcctggcg ataagtctct tcctgatgac
     2281 gcggtacagc ttgtgctgcg accagcaggc gagagcgaat attttcgcgc agatagatat
     2341 cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt
     2401 tgtccataaa gttctgccag cttttttgca gattgatacg ccattcgctg atggaactgg
     2461 aaagctcttc accgtctgaa tccatcggcg aaccatcgct gtcattatcg gccagacgca
     2521 ggttatctac ctgatttgaa agctgattaa gcttaaggat gatgccgtca taatccacct
     2581 gcgatactgc agaaaggctg gcgatatcat cggtaattgc ccgacgaacg gtaatcagac
     2641 tcgggtcatt catatccgcc aggctggcgt ctgcactttt cagcaacgct gcagaggtcg
     2701 tgacgtcctg atcgctccac agcttccgtc cggcgagttt caccagaaaa tcggcctgag
     2761 ccagcagcca ggttttagca tcgctgccgg aaatggtggc gatcttttgt tggacttcat
     2821 ccaactgttt tgccagcgtt tcttgctgac gattcgcctg cttaagctgc gcagcttgtt
     2881 gcttaataat gccttccagc tcggcttttt ggctctcctg ggctttttgc aatgccgtca
     2941 gttggttagc cagggcatcg ctggtggcgg tctgattgac ggcctgttgt ttaccccagc
     3001 catacaaacc gacacccgcc gccagagcaa tagcgatagc caccgcgctg agaatcaatg
     3061 cagtattgtt cttactcttt ttttctgttg cgacaggttg tgacgtggtg tccacggcct
     3121 ccctggtctc ttcaaccacg gcggaggttt tttcttgttc cgtcattatg gcttcctgtt
     3181 atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg gcgaccttaa
     3241 tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg accaatagtc
     3301 gacagtgtag taaccagtgc tcacgatacc attgtgggat cagcgaccag agttgctgca
     3361 acatttcacc gctggtaaca acgaccatcg tcacctcgcg ggattgccag cgcatcgctt
     3421 cttctgcacc atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa gtgacctcag
     3481 caccgcgcac cgtcagggta tccccaatta gctcacgacc gccattgcca cgtaatatca
     3541 gcgcacgttt gcccgcaata ttttgtaatt caggtaattg tagcaagact tcgctgattt
     3601 cccgatcctg tgggtagaga atcttctgtc cgcttacggt atgtagcgcc agtgcggtgg
     3661 tgcgtccaat ggcgaaataa gtaggtagtt ggggccattt acgatcttgc tgatgcagct
     3721 gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg ctctccccca
     3781 gcgctgccag ttgatcagca agttgcggta attgtcgacc cggagaaaac tcaatcagcg
     3841 gaaaatgcca ggccacctgc cccagtgtgc gcagacggct cactaactct tctccagcgg
     3901 gagacgggcg ggtgaccagg atactcatgc cggggcctct ccgttataga cttcagcgag
     3961 gatctcgcgc gcgccgttat tcagtagctc ttctgccagc gaaatcccca tttgttcggc
     4021 atcttgcggc gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg gcgcgccgac
     4081 cagcgcacat agccagattt cgccatcaat aagctcggcg tagctaccaa ttggcacctg
     4141 acatccgcct tcgagacggg tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc
     4201 gtggtgattc agcgcggcaa gcagctcgcg agtgcgtgta tcatcaaggc ggcattcaat
     4261 accgactgca ccttgtccta ccgccggaag agaaatctcg ggtggtaacg cggcgcgaat
     4321 gcgggactcc agacctaaac gttttagtcc ggctacggca agaatgatgg catcgtattc
     4381 gccgttatct agtttgctca ggcgagtgcc gacgttgccg cgcagggagc ggataatcag
     4441 atccgggcgg cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc cgacgatact
     4501 gcctgctggt aacgcatcca gactgtcata gttattggac acaaaggcat cgcgaggatc
     4561 ttcacgctcg caaatagtga ccagccccag accttgcggg aattcaaccg gcacatcttt
     4621 cattgaatgt acggcgatat cagcgcgatt ctccaggagc gcgacttcca gctcttttac
     4681 aaataagcct tttccgccta ctttcgccag cggcgtatca agaatcacat cgccgcgcgt
     4741 caccatcggt accagttcaa cgaccaggcc cggatggctc gccatcaact tgtctttgac
     4801 atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg gcaattctta aaacattgtc
     4861 taacatgctt gttaccgtca ttatcatccg tggtccatcc taacatcctt gccagagtga
     4921 tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg
     4981 gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt acagagaata aacggtgcta
     5041 cacttgtatg tagcgcatct ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt
     5101 tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta
     5161 cctttgacat acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca
     5221 gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct ctatattgag actctgaaac
     5281 agagactgga tgccataaat caattgcgtg tggatcgcgc gcttgctgct atggggcctg
     5341 cattccaaca ggtctacagt ctactgccga cattgttgca ctatcaccat ccgcgaatgc
     5401 cgggttacct tgatggtaac gttcccaaag gcatttgcct ttacacgcct gatgaaactc
     5461 aacgccacta cctgaacgag cttgaactgt atcgtggaat gtcagtacag gatccaccga
     5521 aaggtgagct tccaattact ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa
     5581 gttgttcctc tgacctggat atctgggtct gtcatcaatc ctggctcgat agcgaagagc
     5641 gccagttact acaacgtaaa tgcagcctgc tggaaagctg ggccgcctcg ctgggtgtgg
     5701 aagtcagctt cttcctgatt gatgaaaacc gcttccgtca taatgaaagc ggcagcctgg
     5761 ggggcgaaga ttgtggctcc acccagcata tactgctgct tgacgaattt tatcgtactg
     5821 ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt gccgtgcgac gaagaagagc
     5881 attacgacga ctatgtgatg acgctttacg cgcagggcgt gctgacgcca aatgaatggc
     5941 tggatctcgg tggcttaagc tcgctttctg ctgaagagta ctttggtgcc agcctttggc
     6001 agctctacaa gagtatcgat tccccataca aagcggtgct gaaaacactg ctgctggaag
     6061 cctattcctg ggaatacccg aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc
     6121 acgacggcga gattgtatcg tttggcctcg atccatactg catgatgctg gagcgtgtta
     6181 ctgaatacct gacggcgatt gaagatttca cccgtctgga tttagtacgt cgctgcttct
     6241 atttaaaagt gtgcgaaaaa ctcagccgtg aacgcgcctg tgtaggctgg cgtcgcgcag
     6301 tgttgagcca gttagtgagc gagtggggtt gggacgaagc tcgtctggca atgctcgata
     6361 accgtgctaa ctggaagatt gatcaggtgc gtgaggcgca caacgagctg ctcgacgcga
     6421 tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt agcgtctccg
     6481 ccagtccgca ggatatcggc gtactgacgc gtaaactgta tgccgcgttt gaagcattgc
     6541 caggtaaagt gacgctggta aacccgcaga tttcacccga tctctcggaa ccgaatctga
     6601 cctttattta tgtgccgccg ggccgggcta accgttcagg ttggtatctg tataaccgcg
     6661 cgccaaatat tgagtcgatc atcagccatc agccgctgga atataaccgt tacctgaata
     6721 aactggtggc gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt ttgtatatta
     6781 aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc
     6841 atttcccgct gcgcttacct gcaccgacac cgaaggcgct ctacagcccg tgtgagattc
     6901 gccatctggc gattatcgtt aacctggaat atgacccgac agctgcgttc cgcaatcagg
     6961 tggtgcattt tgatttccgt aagctggatg tcttcagctt tggcgagaat caaaattgcc
     7021 tggtaggtag cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact
     7081 tcaacggcga gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa atgcatcagg
     7141 acgccgcacc gccagatagc gtggaagtct tctgttatag ccagcatctg cgcggcttaa
     7201 tccgtactcg cgtgcagcaa ctggtttctg agtgtattga actgcgcctt tccagcaccc
     7261 gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg ggcttgttct
     7321 tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc catcgagttt tatggcgcta
     7381 tttcgcataa caaactgcac ggcttgtcag tgcaggttga aaccaatcac gtcaaattac
     7441 cggcggtggt ggacggcttt gccagcgaag ggatcatcca gttctttttc gaagaaacgc
     7501 aagacgagaa tggctttaat atctacattc tcgacgaaag caaccgggtt gaggtgtatc
     7561 accactgcga aggcagcaaa gaggagctgg tacgtgacgt cagtcgcttc tactcgtcat
     7621 cgcacgaccg cttcacctac ggctcaagct tcatcaactt caacctgccg cagttctatc
     7681 agattgtgaa ggttgatggt cgcgaacagg tgattccgtt ccgaacaaaa tcgattggca
     7741 acctgccgcc cgccaatcag gatcacgata cgccgctatt acagcagtat ttttcgtgat
     7801 gagcgtgccg gaaagcgagg cttatccggc atgcaatctt agcggaaact gactgtttca
     7861 cccgcctgtt gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg
     7921 caaatccatt catcgccttt caggtcaaaa tggtagccgc cctgtttggt tgccagccac
     7981 acctggtgca gcggctcctg gcggttaata atgattttgc tgccgttttc aaaggtaatg
     8041 gtcagtacgc cgccgttgat ttcgcagtcg atatcgctgt cgccatccct gtcgtccaga
     8101 cgctcttcaa tggtcagcca cagttgatca gccaggcgat gaaattcact gtcgttcatt
     8161 gttgtatcct gtttttaagt gatggcggca gtatagcggc atggggtcag ggcttcaaag
     8221 tttgcacctc tgctgctgcg ttccggcatg attcatccgt caccggaata atgatgtctc
     8281 tgttttgaac ttcacgtacg ttctcctctc tgacacgagt tttcgccggg cagggggagg
     8341 gcgtaatcct tacctctgta tcggtccatg tataaacatt gcgatcgtca taagcgaagc
     8401 gatcgttttt agttagcgaa ccgttaactg gaataatttg caagctcttt gaccgtaatt
     8461 ggcggataaa gtaagcatgc tgtttgccgc tattaaatga gcgatcaatg accaggtctg
     8521 gcgtatccag cgggattatc tcaagctggt cttcaccaaa tgtcgataat tttgttacat
     8581 acagccgatc tttatattgc gcaatattgc cagtaatcag atggaattg
//
LOCUS       DAJTRH010000140         8375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTRH010000140 DAJTRH010000000
VERSION     DAJTRH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..958
                     /gene="espL1"
                     /locus_tag="OO339_003620"
                     /pseudo
     CDS             1..958
                     /gene="espL1"
                     /locus_tag="OO339_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1130..1237
                     /locus_tag="OO339_003621"
     CDS             1130..1237
                     /locus_tag="OO339_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928104.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1290..2048)
                     /locus_tag="OO339_003622"
     CDS             complement(1290..2048)
                     /locus_tag="OO339_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5928105.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2335..3264
                     /gene="pfkB"
                     /locus_tag="OO339_003623"
     CDS             2335..3264
                     /gene="pfkB"
                     /locus_tag="OO339_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5928106.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3365..3655
                     /gene="ghoS"
                     /locus_tag="OO339_003624"
     CDS             3365..3655
                     /gene="ghoS"
                     /locus_tag="OO339_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5928107.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3761..4621
                     /locus_tag="OO339_003625"
     CDS             3761..4621
                     /locus_tag="OO339_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5928108.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4662..5198)
                     /locus_tag="OO339_003626"
     CDS             complement(4662..5198)
                     /locus_tag="OO339_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5928109.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5345..6013
                     /gene="hxpB"
                     /locus_tag="OO339_003627"
     CDS             5345..6013
                     /gene="hxpB"
                     /locus_tag="OO339_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5928110.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6176..6766
                     /locus_tag="OO339_003628"
     CDS             6176..6766
                     /locus_tag="OO339_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5928111.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6899..8289
                     /gene="tcyP"
                     /locus_tag="OO339_003629"
                     /pseudo
     CDS             6899..8289
                     /gene="tcyP"
                     /locus_tag="OO339_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2172 a         2003 c         2009 g         2191 t
ORIGIN      
        1 gatgctcaga cctgggctga taggtatgtt cgcaactgtg caattgctat tctgccccta
       61 ttacctgtgg gagttctagg ggctattttc gcgggtattg catcacgaat gccatttgcc
      121 cctatacatc catcggcaat gttgttaata atggctacag gccagactca acagcttatt
      181 acattattca aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg
      241 cagaatagcg ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac
      301 gtgaaaatat ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat
      361 aatctggtta aattactgca aactaaaagt gctaacgaaa cacctggact ttatatctcc
      421 atgttgtatg gattcgatga aataatcgat atctttctga atacattagc cactccaata
      481 gcactaagag cttttaaaca aaaaactggt gatgagtatt ttagccatga aaatacatga
      541 tggtgagcca ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt
      601 cctctctaaa attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga
      661 tttattaaaa ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg
      721 taatgaagac gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc
      781 ttttagtcaa catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc
      841 tgttcatata gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat
      901 aaatgtcatc agccaaagtc tgagttttag tgctgatgaa ataaagacgt atttataacc
      961 agctatttac gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt
     1021 gaccatgtgc acaacgccac ccgacggctg taataacaaa aaatcccttg atgcctgtcc
     1081 cttttgttac actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa
     1141 acgctttgat aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat
     1201 gttgttgcta cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca
     1261 gaccgcataa cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc
     1321 cgacggtcgg tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa
     1381 gccaccttca gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc
     1441 gttgtgtctt ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac
     1501 tgccatgcat aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta
     1561 tatttgtcgt aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc
     1621 aaaaactgac gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg
     1681 tctgtcagcc agcttgcctg accaaataaa tagtcataat cagttaaatt aaagcggcta
     1741 cgcccgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
     1801 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
     1861 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
     1921 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
     1981 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
     2041 agcttcattt atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt
     2101 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
     2161 acttagcaat gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta
     2221 tttatatata tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt
     2281 cttcactttc cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta
     2341 cgtatctata cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt
     2401 tatcccgaag gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc
     2461 aacgtcgccc gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt
     2521 ggcgcgaccg gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact
     2581 gtagaagcca aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa
     2641 cagtatcgtt ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa
     2701 gagcaagttc tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca
     2761 ggtgtgaagc tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc
     2821 tgcatcatcg acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag
     2881 ttggttaagc ctaaccaaaa agaactcagt gcgctggtga atcgcgaact cacccagcca
     2941 gacgatgtcc gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc
     3001 gtttcccttg gtccacaagg agcgctgggt gtcgatagtg aaaactgcat tcaggtggtg
     3061 ccaccaccgg tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg
     3121 acactgaaac tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg
     3181 gggagtgcag ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa
     3241 atttacgctt acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc
     3301 aacctgtcgg caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg
     3361 aactatggcc agtggcgatc ttgtccatta tgtcataacc gtcatgttgc atgaggatac
     3421 attgactgaa attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat
     3481 gacggatgat gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac
     3541 ccaaagtgaa gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa
     3601 agatcctgaa atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt
     3661 ttttgggcaa taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc
     3721 gctaacctta tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg
     3781 tcttttgagc gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg
     3841 agaagtccat gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga
     3901 tgaaagggaa ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg
     3961 tagtaaaacc gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt
     4021 tctggtgatg gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg
     4081 tcagcaaatt gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga
     4141 taactcgctc tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt
     4201 tgctgaacaa cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg
     4261 caatatcgac gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc
     4321 ttctctgttg cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta
     4381 cattttcgac cctgcctgcg actggggtga ccgagagtgc gacctagcga tgttaccgct
     4441 gcatactgaa cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc
     4501 cgatttcctt gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt
     4561 atttggcggt cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg
     4621 atatgggttg aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga
     4681 gaaaagaaca cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac
     4741 tgcaggaaaa tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc
     4801 ccctcttccc ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag
     4861 cgcgcctggc gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg
     4921 atgaaaatca cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta
     4981 taccagaaca gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca
     5041 aagtcgagca tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc
     5101 agcaaagaaa tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt
     5161 ttcaaaacag caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc
     5221 gtcgtttcac ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg
     5281 ataggcgctt aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt
     5341 tgtaatgtca accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat
     5401 cgactcagaa cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga
     5461 tatctcccgt cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga
     5521 tctttggtac gcccggcaac cgtggaatgg gccaagccgt caggaagtag tagaacgggt
     5581 tattgcccgt gccatttcac tggttgaaga gacacgtcca ttattaccag gcgtgcgcga
     5641 agctgttgcg ttatgcaaag aacaaggttt attggtggga ctggcctccg cgtcaccact
     5701 acatatgctg gaaaaagtgt tgaccatgtt tgacttacgc gacagtttcg atgccctcgc
     5761 ctcggctgaa aaactgcctt acagcaagcc gcatccgcaa gtatatctcg actgcgcagc
     5821 aaaactgggc gttgacccac tgacctgcgt ggcgctggaa gactcggtaa atggcatgat
     5881 cgcttctaaa gcagcccgca tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga
     5941 tccacgtttt gtattagcaa acgtcaaact ctcatcgctg acagaactca ccgcaaaaga
     6001 ccttctcggt taatgaccag gggcagtgat cacgctgccc ctggttcttt atctgaattt
     6061 gcattcaact gacggattaa tcgtcaattt aagagaaaga gttacaccgt caccacttcc
     6121 gtgcactgta taaaaatcct atactgtacg tatcgacagt ttagtgagtt ttatcatgac
     6181 ggcggaaggt caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct
     6241 gacgcccgtg ctggcacagg gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg
     6301 tttgttaccg gacatcgatc acccaaagtc gtttcttggg cagcgattaa aatggatatc
     6361 aaaaccgatc gcccgcgctt ttgggcatcg tggttttacc cacagtctgc tggcggtatt
     6421 tgcgctgctg gcaacctttt accttaaggt tccggaaggc tggttcattc cggctgatgc
     6481 gctacaagga atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc
     6541 cggtgttccc ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca
     6601 aaagggcaac caactggaac gttttatctg catggcatta tttgtctggt cggtatggat
     6661 gccccattca ttacccgaga acagcgctgt tcgttggtca tcgcaaatga tcaatacctt
     6721 gcagatccag tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatca
     6781 cctttctgga ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct
     6841 tgcgccaaca ctatgactgc tacgcagtga tagaaataat aagatcagga gaacggggat
     6901 gaactttcca ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca
     6961 gacccgccat aaacagtgga gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt
     7021 ggtttttggc cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc
     7081 tgtacagtgg tttaacatcg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat
     7141 gccgttagtc ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt
     7201 aggcaaaatc agttttctga ccatcggtac gcttttgttt accacgctga ttgcggcgct
     7261 ggtcggtgtg ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg
     7321 tgcagaaact gcacgtctga acgccattga aagtaactat gttggtaaag tctctgatca
     7381 gagcgttccg cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg
     7441 agccaatccg acgtcaatta tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc
     7501 attgaaactg ctgaaggatg atgcgccgaa aggggaacgc gtcttaaccg ctatcgatac
     7561 cctacaaagc tgggtgatga aactggttcg ccttgtcatg cagttgaccc cttacggcgt
     7621 tctggctcta atgaccaaag tggttgcagg ttctaacttg caagacatca tcaaactggg
     7681 aagtttcgtt gtcgcgtcct acctcggtct gctgattatg tttgctgtgc atggcattct
     7741 gctgggcatt aatggcgtga gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac
     7801 gtttgccttt accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac
     7861 gcgtcgtctg ggcgttcctg aatccatcgc cagtttcgcc gcctctttcg gtgcaaccat
     7921 tggtcagaac ggctgcgccg gtttgtatcc ggcaatgctg cggtgatggt tgcgcctacg
     7981 gttggcatta acccgctgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt
     8041 agttccgcag gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg
     8101 cctgcgatgg gcctgccagt aaccctggtg gcgctgttaa tctccgttga accgcttatc
     8161 gacatgggcc gtacggcgct aaacgttagt ggctcgatga cagctggcac gctgaccagc
     8221 cagtggctga agcaaaccga taaagccatt ctggatagcg aagacgacgc cgaactggca
     8281 caccgttaat tcttatgctg gcaacggtcc gttttgtata ggggccgttg ctttactttt
     8341 taaatgttcc atcgcggcga tgcatactga ctcat
//
LOCUS       DAJTRH010000141         8360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTRH010000141 DAJTRH010000000
VERSION     DAJTRH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(171..791)
                     /gene="entD"
                     /locus_tag="OO339_003630"
     CDS             complement(171..791)
                     /gene="entD"
                     /locus_tag="OO339_003630"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5928112.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(966..3206)
                     /gene="fepA"
                     /locus_tag="OO339_003631"
     CDS             complement(966..3206)
                     /gene="fepA"
                     /locus_tag="OO339_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5928113.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3449..4651
                     /gene="fes"
                     /locus_tag="OO339_003632"
     CDS             3449..4651
                     /gene="fes"
                     /locus_tag="OO339_003632"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5928114.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4654..4872
                     /gene="ybdZ"
                     /locus_tag="OO339_003633"
     CDS             4654..4872
                     /gene="ybdZ"
                     /locus_tag="OO339_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5928115.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            4869..>8360
                     /gene="entF"
                     /locus_tag="OO339_003634"
     CDS             4869..>8360
                     /gene="entF"
                     /locus_tag="OO339_003634"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5928116.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQE"
BASE COUNT         1798 a         2245 c         2300 g         2017 t
ORIGIN      
        1 acgtaatatt gagtttaagg ctgttcttgc ttacgaaccg aagaagtagc cgttgtgcgg
       61 ggagtaatcc ccgcatatcc ggttgtcagg tcagagtggt aaggcacctg ctttacactt
      121 tcgcccgcag tcagtgatgg ctgcgggcga atcgtaccag atgttgtcaa ttaatcgtgt
      181 tggcacagag ttatgactat tttttctttt atctgccagt gcacagcaaa catctcattc
      241 tcacgatgaa tgatgacctg ctgtttattc cagctaatta tctgatagtc cagaaaacct
      301 gcatcagttt ggatctcact tgccttaaat gcgctctctt tggcggaaaa tgcaagtgtc
      361 agcgccagag aaaaggctaa accgcagtct gcgagtcgct cgtgttctgc tggtgtaata
      421 atgttgtctg tcaattcggt cgctgtttgc gcagaaaaaa tttcttcgat atcaacgcca
      481 atcggttgac gggaaaccac ggccaatgcc gtagccccgc agtggctaat actgccgtat
      541 acctccgcag gccacaccgg ttggcgtagc tcgccgattg cgggcacaca tttatagcca
      601 tattcccgca aggcataaac agcagcgatc cgtccggcta aatgctctgt tttacgttta
      661 cgtccagcgt gttgcagttg tgcgtagtgc ggcagccaga gtaaatcctg ctcacaaaaa
      721 ttcgccggat cgaactcaac aaaatgcagc gtatgtccgg caaaggggag ggaggtatgc
      781 gtagttttca tatcgaccat attcgagact gatgacaaac gcaaaactgc ctgatgcgct
      841 acgcttatca ggcctacgtg atcctgcaat atattgaatt ggcaagatct ttgtaggccg
      901 gataaggcgt tcacgccgca tccggcgtga acgacgcgca ctttgtcaac aatctgacgt
      961 tagcatcaga agtgagtatt aatgctcata taccacgtac gtcccggctc gttataggta
     1021 tacgcgccgg caccggcgat atagttggct cctgccagat cgcccgtggt ctgggcatta
     1081 cctgcacgcc acaaacgttt gtcgaacagg ttgtccacgc cgccggtcag actgacattc
     1141 ttcgtcacat cccaggtcgc gctcagaccc acaatgctgt aaggactgat ttctttggtt
     1201 tccgggccaa ccgctggctg acctttatag ttgtacttct tcggctgctg cttgccgtac
     1261 caggtgaagg tcgtttgcat cgacaaatct tcccgtgcct gccagctcag cgttgagttc
     1321 aacgtgtact ccgggatgat cgacaaacgg tcgcccgtgg ttttgttttc actcttcagc
     1381 atataagtga tgttattggt ccacatcacc gtttcgctaa ctggtacgtt taacgatcct
     1441 tccagacctt caaccactgc tttcggcacg ttatcccact gatagagatc agtgccgact
     1501 gcgttttgcc ctacagccac atagcccgct tcaatcttat tgcgataatc gttacggaac
     1561 caggtgacgc ccgccagcca gccgtcgcgt ttgaactcca gaccaatctc tttgttgatg
     1621 ctggtttcta ctttcaggtc atcgttacct tgcagatagc agccgcccgc gctggcatag
     1681 cagccctgac ctttactgta gagaatgtag ttcgggttag tctggtatag gctcggcgct
     1741 ttataggcgc gggcaatgcc tattttcagc gtgaagtcat cgcctaaacc ttgcgatatg
     1801 ttcagcgccg ggctccagtt attgccgaca atactgtgat gatcgaaacg cagccccggc
     1861 gttacgatgg tgctgtcagt cagctccatg ttgttttcgg caaacagcga gaaaatttct
     1921 gcttttgaat acgggctacg gtcggtggca ctcacgccat caatagcgcc accggtattc
     1981 gttccggtca gtgcttgggt gttggaactt aagtccttca tccgttgctg attccactcc
     2041 gtacccagcg tcagcgtctg gttaacgagg aaatcaatcg gcaggttaac ttcgctgtgc
     2101 agcatcacgt catcgagatc gatatcgacg aaatcctgtg tcgctttttc gttaaatttc
     2161 ccttcggtac cgcccgccag accttccgga atacgcgagt tacgggtgtg ttcatactgc
     2221 acccagttgc tggtggtcac gccgttatcc cagccaccgt tccaggtcag cgcgtagttc
     2281 tggcgataca ggcggttggt ttcatcgcca tatttcgagc gagtgtaagc gtcagagttg
     2341 gtgttctggg tatcgcccgc atacaggtta ccctggcggc tgtaacccgc ttccagttcc
     2401 agcgattgca atggtgcgaa gtcccagcgc accacgccgt taatatcttt attgatcacc
     2461 ccttcgcgcc cggctggtaa cgtcgtggca tacgttccgg cacgcgcgga ctgatggccc
     2521 tggttgatat cccacgcgtc agcctgagtt ttgtcgaggt tgccatacaa gcggaagctg
     2581 aattcgtcgc ccagcggacc ggtcaggcta aagttagtgc gtttggtggc accttcctct
     2641 ttatgttctg gcgcattgaa atatgcgtcc caggagccgt gccactcgcc gctgcctttt
     2701 ttggtaatga tgttaaccac gccgcccgcc gcgccgttgc cataacgcgc agctgccgga
     2761 ccacgcagaa cttcaatacg ttcaatcatt tcaggtggca cccaggaggt atcaccacgg
     2821 gtatcgcgct cgccacgcca gccctgacgc accgagttac ggctgcttac cggcttgccg
     2881 tcaatcaaaa tcagcgtgtt ttccggaccc ataccgcgaa tatcaatctg tcggttattc
     2941 ccacgctgac cactggtgga gttaccggtc aggttaacgc ctggcatggt acggatgatc
     3001 tccgacacat cgcgggcaac cgggtttttg cggatttcat ctgcggtgat ggtcgaaacg
     3061 ccaggagcct gcaagttctg ctcggcggcg gtaacgacaa tggtatcgtc atgtgaaaca
     3121 ggtgtatcgg tcggctcttg tgcctgcgct accccataaa tccccagatt gaccaacaag
     3181 gccagggaat gaatcttctt gttcattgtt ttattcctgc atttttgcca cgaattgcaa
     3241 ctgtcgggca tggtcgtcat caacacgacg catcccgtta ccgcgaaaac ctttgatcct
     3301 gaaagacacg cagtgcagtt ggttaattaa tgtccgcgct tcccacggcg cgccattacg
     3361 ctattgcaaa tgcaaatagt tattaataac attatcaata tatttctgca atcaatgaaa
     3421 aattgcacag taaacatggg gttatggtgt gacggcgtta aaagtaggaa gtgagagctg
     3481 gtggcagtcg aaacatggcc cggaatggca gcgtctgaat gacgaaatgt ttgaggtcac
     3541 tttctggtgg cgtgatcccc aaggttctga agaatactcg acgataaagc gcgtatgggt
     3601 ctacatcact ggtgtgaccg atcatcatca gaacagccag ccccagtcga tgcagcgaat
     3661 tgcaggtact gatgtctggc agtggacgac acaactcaat gccaactggc gcggcagcta
     3721 ctgctttatt cccaccgaac gcgatgacat tttttctgca ccatcccccg atcgcctcga
     3781 attacgtgaa ggctggcgaa aactattacc ccaggcaata gccgatccgc tgaatccaca
     3841 aagctggaaa ggcgggctag ggcacgctgt ttctgcactc gaaatgccgc aagcgcctct
     3901 gcaaccggga tgggattgtc cgcaagcgcc agaaatacca gccaaagaaa ttatctggaa
     3961 aagtgaacgg ttgaaaaatt cacgtcgtgt atggattttt accaccggcg atgtaacagc
     4021 agaagaacgc ccgctggcag ttttgctcga tggcgaattt tgggcgcaaa gtatgcccgt
     4081 ctggcctgtg ctgacttcgc tgacccatcg tcagcaactt cctcccgccg tgtatgtgtt
     4141 gatcgacgct atcgacacca cgcaccgcgc ccacgaactg ccgtgtaatg cggatttctg
     4201 gctggcagta cagcaagagt tattacccct ggtgaaagtt attgcccctt ttagcgatcg
     4261 cgccgatcgc accgtggtcg ccgggcagag ttttggtggg ctgtccgcgc tgtatgccgg
     4321 actgcactgg cctgaacgct ttggctgtgt attaagccag tcaggatcgt actggtggcc
     4381 gcatcggggc gggcagcaag agggcgtgtt acttgaaaag ctaaaagctg gtgaagttag
     4441 cgccgaaggt ctgcgcattg tgctggaagc gggtattcgc gagccgatga tcatgcgggc
     4501 caatcaggcg ctgtatgcgc aattacaccc tataaaagaa tccattttct ggcgtcaggt
     4561 tgacggcgga catgatgcgc tttgttggcg cggtggcttg atgcaggggt taatcgacct
     4621 ctggcaacca cttttccatg acaggagttg aatatggcat tcagtaatcc cttcgatgat
     4681 ccacagggag cgttttacat tttgcgcaat gcgcaggggc aattcagtct gtggccgcaa
     4741 caatgcgcct taccggcagg ctgggacatt gtgtgtcagc cgcagtcaca agagtcctgc
     4801 cagcagtggc ttgaagccca ctggcgtact ctgacaccgg cgaatttcac ccagttgcag
     4861 gaggcacaat gagccagcat ttacctttgg tcgccgcaca gcccggcatc tggatggcag
     4921 aaaaactgtc ggatttaccc tccgcctgga gcgtggcgca ttacgttgag ttaaccggag
     4981 aggttgatgc gccattactg gcccgcgcgg tggttgccgg attagcgcaa gcagatactt
     5041 tgcggatgcg ttttacggaa gataacggcg aagtctggca atgggtcgat gatgcgctga
     5101 tattcgaact gccagaaatt atcgacctgc gaaccaatat tgatccgcac ggtactgcgc
     5161 aggcattaat gctggcggat ttgcaacaag atttgcgcgt cgatagcggt aaaccactgg
     5221 tctttcatca actgatacag gtggcagata accgctggta ctggtatcag cgttatcacc
     5281 atttactggt cgatggcttc agttttccgg ccattacccg ccagatcgcc aacatttact
     5341 gcgcattgct gcgtggcgaa ccaacgcctg cttcgccgtt tacgcctttc gctgatgtag
     5401 tggaagagta ccagcaatac cgcgaaagcg aagcctggca gcgtgatgcg gcattctggg
     5461 tggaacagcg tcgccaactg ccaccgcccg cgtcactttc tccggcacct ttagcggggc
     5521 gcagcgcctc ggcagatatt ttgcgtctga aaatggaatt taccgacggg gaattccgcc
     5581 agctggctac gcaactttca ggtgtgcagc gtaccgattt agcccttgcg ctgacggctt
     5641 tgtggctggg gcgattgtgc aatcgcatgg actacgccgc cggatttatc tttatgcgtc
     5701 gactgggctc ggcggcgctg acggctaccg gacccgtgct caacgttttg ccgctgggta
     5761 ttcatattgc ggcgcaagaa acgctgccgg aactggcaac ccgactggca acccgactgg
     5821 cagcacaact gaaaaaaatg cgtcgtcatc aacgttacga tgccgaacaa attgtccgtg
     5881 acagcgggcg agcggcaggt gatgaaccgc tgtttggtcc ggtactcaat attaaggtat
     5941 ttgattacca actggatatt cctggtgttc aggcgcaaac ccataccctg gcaaccggtc
     6001 cggttaatga ccttgaactg gccctgttcc cggatgaaca cggtgatttg agtattgaga
     6061 tcctcgccaa taaacagcgt tacgatgagc caacgttaat ccagcatgct gaacgcctga
     6121 aaatgctgat tgcccagttc gccgcggatc cggcgctgtt gtgcggcgat gtcgatatta
     6181 tgctgccagg tgagtatgcg cagctggcgc agatcaacgc cactcaggtt gagattccag
     6241 aaaccacgct gagcgcgctg gtggcagaac aagcggcaaa aacaccggat gctccggcgc
     6301 tggcagatgc gcgttacctg ttcagctatc gggaaatgca cgagcaggtg gtggcgctgg
     6361 cgaatctgct gcgtgagcgc ggcgttaaac caggggacag cgtggcggtg gcactaccgc
     6421 gctcggtctt tttgaccctg gcactccatg caatagttga agctggcgcg gcctggctac
     6481 cgctggatac cggctatccg gacgatcgcc tgaaaatgat gctggaagat gcgcgtccgt
     6541 cgctgttaat taccaccgac gatcaactgc cgcgctttag cgatgttccc aatttaacca
     6601 acctttgcta taacgccccg cttacccccc agggcagtgc gccgctgcaa ctttcacaac
     6661 cgcaccacac ggcttatatc atctttacct ctggctccac cggcaggccg aaaggggtaa
     6721 tggtcgggca gacggctatc gtcaaccgcc tgttgtggat tcaaaatcat tatccgctca
     6781 caggcgaaga tgtcgttgcc caaaaaacgc cgtgcagttt tgatgtctcg gtgtgggagt
     6841 ttttctggcc gtttattgcc ggggctaaac tggtgatggc tgaaccggaa gcgcaccgcg
     6901 acccgctcgc tatgcagcaa ttctttgccg aatatggcgt aacgaccacg cactttgtgc
     6961 cgtcgatgct ggcagcattt gttgcctcgc tgactccgca aaccgctcgc cagagttgcg
     7021 tgacgttgaa acaggttttc tgtagtggtg aggccttacc ggctgattta tgccgcgaat
     7081 ggcaacagtt aaccggcgcg ccgttgcata acctgtatgg cccgacggaa gcggcggtag
     7141 atgtcagctg gtatccggct tttggcgagg aactggcaca ggtgcgcggc agcagtgtgc
     7201 cgattggtta tccggtatgg aatacgggtc tgcgtattct tgatgcgatg atgcatccgg
     7261 tgccgccggg tgtggcgggt gatctctatc tcaccggtat tcaactggcg caggggtatc
     7321 ttggacgacc cgatctgacc gccagccgct ttattgccga tccttttgct cctggtgaac
     7381 ggatgtatcg taccggagac gttgcccgct ggctggataa cggcgcggtg gagtacctcg
     7441 ggcgcagtga cgatcagcta aaaattcgcg ggcagcgtat cgaactgggc gaaatcgatc
     7501 gcgtgatgca ggcgctgccg gatgtcaaac aagccgttac ccacgcctgt gtgattaacc
     7561 aggcggcagc cactggtggt gatgcgcgtc agttggtggg ctatctggta tcgcaatcgg
     7621 gcctgccgtt ggataccagc gcattgcagg cgcagcttcg cgaaacattg ccgccgcata
     7681 tggtgccagt cgttctgctg caactgccgc agttgccact tagcgccaac ggcaagctgg
     7741 atcgcaaagc cttaccgttg cctgaactga aggcacaagc gccagggcgt gcgccgaaag
     7801 cgggcagtga aacgattatc gccgcggcat tcgcgtcgtt gctgggttgt gacgtgcagg
     7861 atgccgatgc tgatttcttt gcgctgggcg gtcattcgct actggcaatg aaactggcag
     7921 cgcagttaag tcgacagttt gctcgtcagg tgacgccggg gcaggtgatg gtcgcgtcaa
     7981 ccgtcgccaa actggcaacg attattgatg gtgaagagga cagctcccgg cgcatgggat
     8041 tcgaaaccat tctgccgttg cgtgaaggta atggcccgac gctgttttgt ttccatccgg
     8101 cgtccggttt tgcctggcag tttagcgtgc tctcgcgtta tctcgatcca caatggtcga
     8161 ttattggcat tcagtcgccg cgccctcatg gacccatgca gacagcgaca aacctggatg
     8221 aagtctgcga agcgcatctg gcaacgctac ttgagcaaca accgcacggc attgcggcgc
     8281 ggctgcgtgc ccgtggcgaa caggtggcat ttcttggttt gctggatacc tggccgccag
     8341 agacgcaaaa ctggcaggaa
//
LOCUS       DAJTRH010000142         8332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTRH010000142 DAJTRH010000000
VERSION     DAJTRH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..159
                     /locus_tag="OO339_003635"
     CDS             <1..159
                     /locus_tag="OO339_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928117.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO339_003636"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO339_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5928118.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO339_003637"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO339_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5928119.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="OO339_003638"
     CDS             875..1246
                     /locus_tag="OO339_003638"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5928120.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1267..6128
                     /gene="traI"
                     /locus_tag="OO339_003639"
                     /pseudo
     CDS             1267..6128
                     /gene="traI"
                     /locus_tag="OO339_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="OO339_003640"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="OO339_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5928121.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="OO339_003641"
     CDS             6949..7509
                     /locus_tag="OO339_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5928122.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="OO339_003642"
     CDS             7640..7852
                     /locus_tag="OO339_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5928123.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8332
                     /locus_tag="OO339_003643"
     CDS             8096..>8332
                     /locus_tag="OO339_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5928124.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2671 g         1662 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca ca
//
LOCUS       DAJTRH010000143         8310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTRH010000143 DAJTRH010000000
VERSION     DAJTRH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..190
                     /gene="csgC"
                     /locus_tag="OO339_003644"
     CDS             <1..190
                     /gene="csgC"
                     /locus_tag="OO339_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5928125.1"
                     /translation="GSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWTPSSEKS"
     gene            311..622
                     /locus_tag="OO339_003645"
     CDS             311..622
                     /locus_tag="OO339_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5928126.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            717..1250
                     /gene="ymdB"
                     /locus_tag="OO339_003646"
     CDS             717..1250
                     /gene="ymdB"
                     /locus_tag="OO339_003646"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5928127.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1192..2673
                     /gene="clsC"
                     /locus_tag="OO339_003647"
     CDS             1192..2673
                     /gene="clsC"
                     /locus_tag="OO339_003647"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5928128.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2681..3838)
                     /gene="mdoC"
                     /locus_tag="OO339_003648"
     CDS             complement(2681..3838)
                     /gene="mdoC"
                     /locus_tag="OO339_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5928129.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4232..5767
                     /gene="mdoG"
                     /locus_tag="OO339_003649"
     CDS             4232..5767
                     /gene="mdoG"
                     /locus_tag="OO339_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5928130.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5760..8303
                     /gene="mdoH"
                     /locus_tag="OO339_003650"
     CDS             5760..8303
                     /gene="mdoH"
                     /locus_tag="OO339_003650"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5928131.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2101 a         1969 c         2172 g         2068 t
ORIGIN      
        1 aggcagttca gggcaaagtc agacgaagca agaaaagacc ctctcattgc ctgctaatca
       61 acccattgct ttgacgaagt tgagtttaaa tatttccccg gaagatcggg tgaaaatagt
      121 tgttactgtt tctgatggac aggcacttca tttatcacaa caatggacgc cctcttcaga
      181 aaagtcttaa tttgttgaaa tatcgagcat aagatgaatc tggagagaat ggtctgctgc
      241 gaatcagcca acctgaaagt atggataaca caaccttcaa ggatgactaa tcattgagga
      301 aatagaataa atgttcagac cttttttaga ctctcttatg ctcggcagtt tgttttttcc
      361 ttttattgcc attgcaggaa gcaccgcgca agggggcgtg atccattttt atggccaaat
      421 tgtggaaccg gcatgtgacg tcagcaccca gtcatcaccc gtagaaatga actgcccaca
      481 gaatggttct attccgggca aaacctactc cagcaaagcg ttaatgagcg gcaatgtcaa
      541 aaacgcgcaa atagcctcag taaaagtgca gtatcttgat aaacagaaaa agctggctgt
      601 aatgaacatc gaatataact aagtttctga acaactcacg ttgctgagca gaaaaatgcg
      661 atttaaccaa aaagcctgct gtacacttaa gaaacaagaa ggtgtaagga ggcgttatga
      721 aaacgcgtat tcatgttgtg cagggtgata ttaccaaact ggccgttgat gtgattgtga
      781 atgcggctaa tccgtcatta atgggaggcg gcggcgtcga tggggccatt catcgcgcag
      841 cgggtccggc cctgctggat gcctgtttaa aagtcaggca acagcagggc gattgcccta
      901 cggggcatgc cgttattacg cttgcaggcg atcttcccgc taaagccgta gtacacaccg
      961 tcgggccagt ctggcgtggt ggggaacaaa acgaagacca gcttttgcag gatgcctatc
     1021 tgaatagcct acgactggtg gcggcaaaca gctatacgtc agtggctttt cctgcaatca
     1081 gtactggggt ttatagttac cctcgtgcgg cagcggctga aatcgcagtt aaaaccgttt
     1141 cagaatttat tacccgtcac gctttacccg aacaggtata ctttgtctgt tatgatgaag
     1201 aaaacgccca cctctacgaa agactcctta cccaacaagg agatgaatga tttgccccgg
     1261 ctggcgagcg cggtgctgcc actgtgttcg caacatcccg gtcagtgtgg tcttttccct
     1321 ctggagaaaa gtctggatgc gtttgccgcc cggtatcgtc tggccgaaat ggcagagcat
     1381 acgctggatg ttcagtatta catctggcag gacgatatgt cggggcggtt actgttttcc
     1441 gccctgttag ccgcagcaaa gcgtggcgtt cgcgtccgtt tgttgctgga cgacaacaat
     1501 acgcccggac ttgacgacat tttgcgcttg cttgacagtc atccacgcat tgaagtccgg
     1561 ctttttaatc ctttctcgtt tcgcttgcta cgtccgcttg gatatatcac cgacttttcc
     1621 cgtctcaatc gccggatgca caataaaagt ttcactgtcg atggcgtggt taccctagtg
     1681 ggaggacgaa atattggtga tgcctatttt ggtgcagggg aagagccact tttttcggat
     1741 ttagatgtca tggcaatagg ccccgtggta gaggatgttg ccgatgattt cgcccgctac
     1801 tggtattgca aatcggtttc acccttacag caggtgctgg atgtcccgga aggtgaaatg
     1861 gcggatcgca tcgagttacc cgcctcctgg cataacgatg ccatgacgca tcgttattta
     1921 cgcaaaatgg aatccagtcc atttataaat catctggttg atggaacgtt gccgcttatc
     1981 tgggcgaaga cacgtttatt aagtgatgat ccggcgaaag gggagggcaa ggcaaaacgg
     2041 cattcactgt taccgcagcg cctgttcgat atcatgggct cacccagtga acgcatcgat
     2101 attatctctt cctattttgt accgacacgc gcaggtgtgg cgcaactctt acggatggtg
     2161 agaaaagggg taaagattgc gatcctaacc aattctcttg ccgctaacga tgttgctgtc
     2221 gtccatgccg gatacgcgcg ctggcgcaaa aaattgctcc gctatggcgt ggaattatat
     2281 gaactcaagc cgacgcgtga acaaagcagt acgttacacg atcgcggcat aaccggtaat
     2341 tccggtgcca gcctgcatgc taaaactttt agcatcgatg gtaaaacggt gtttatcggc
     2401 tctttcaatt tcgatccgcg ttcaacattg ctcaatactg aaatgggctt cgtgatagag
     2461 agcgaaacgc tggcacagtt aattgataaa cgctttattc agagccagta cgatgcggcc
     2521 tggcagctcc gtctggacag gtggggacgg atcaactggg ttgatcgtca tgcaaagaaa
     2581 gaggttattc tcaaaaaaga acccgccacc agtttctgga agcgggttat ggtcagactg
     2641 gcgtcgatat tgcccgtgga atggttattg taaatgtggc ttaacgggct ggtgctttat
     2701 cgttctcacg cttaacaacc ggtttaccag agaacaaaaa cttcagtaac gggatgcgta
     2761 aatgaatttc atacagaatt atcgcaatcc ctactacgaa tatcaggcca cagagaaaac
     2821 caagccagtt ggaggtgatg tgcggtgtaa tgtatgcgcc gaaaaacagc gttaacgggt
     2881 ggtgaaccag atagataaac agcgatgcgt taacaaaata agtcacccgc gctgactgga
     2941 agttaagcaa acggtggcca aaggagaaga ccacattcac catccacagg ccgaggacca
     3001 tggtgatcac cgactcggtt tcgtacatcc aggcatcgcc actgccatag cgctggttga
     3061 gtaaataagc gacaaacgcc aatgctgcag cgagggtaca gccacgagac ggcgtggtaa
     3121 acaaggcttt aagatgaggg aaaatgaaag ccagtgcgcc gaggataaag aacggtaaat
     3181 aaaatagcgt ttgcatgaca ataaaattga acatgccatt actcagaatg ggtggataca
     3241 caataaaaat cgttcttctt attaccgcat agccgatgcc gaggcataaa aaaatcaccg
     3301 atagttttac catcgagaat tttttattcg ttttatcaga attttctaaa ttatttctga
     3361 tgcgcttaaa tatccataca cacagcgtcg tcatgactac taacaccagt aaaaaccaca
     3421 ggtgtgatat taattcccag gccaacgtat tatatttgtc atacaatgat agcccaggcc
     3481 aactttccgc tttccctttg acatattgca gcataataaa ttgcggtaat gtcagtaggg
     3541 ggatggctgt taacatcggg atacctacac gttcgacacg tactttccac cattttttca
     3601 agggatagcg taaaaaaagc atgtaggaaa agtatccgga tataacgaaa aatacctgca
     3661 tgcggaacga gtggatgaag tcattaaaaa gggttagcca caatgacggt tcggcgctat
     3721 tcacatgcca tgtatggctc gaatagatta aagaaatatg aaaagggatc cctaacaaca
     3781 tcagccaggc gcggatggag tcgaggaaat attcacgttg cgcgggtact ggattcatat
     3841 atggttaact aatctcggat ttttcgtctt atccctgtcg ggttatgcct ttaggcttgt
     3901 tgccatagcg acaccgacct gaccgcgcca ggcgcaggct tcaaggtttt tatgcatagc
     3961 atcatcgcta ccactaacca gaatggaagc gtctgtaaga cggttgataa ataaatttgc
     4021 tggcaaaccc tacacgaagt cgatgcttct gtctttagga gaagcacgga aagtgaaaac
     4081 ggttgcaatc aggtgcttaa tccatgagcc agcgtgctga acgataccgg gattcagttg
     4141 tcggaatggc tggttatcca ttaaaataga tcggatcgat ataagcacac aaagggggaa
     4201 gtgcttacta attatgaaac ataaactaca aatgatgaaa atgcgttggt tgagtgctgc
     4261 agtaatgtta accctgtata catcttcaag ctgggctttc agtattgatg atgtcgcaaa
     4321 gcaagctcaa tccttagccg ggaaaggcta tgaggcgcct aaaagcaact tgccctccgt
     4381 tttccgcgac atgaaatacg cggactatca gcagatccag tttaatcatg acaaagcgta
     4441 ctggaacaat ctgaagaccc cattcaaact cgagttctac catcagggta tgtacttcga
     4501 taccccggtc aaaataaatg aagtgactgc caccgcagtc aaacgaatca aatacagccc
     4561 ggattatttc actttcggcg atgttcagca tgacaaagac acggtaaaag accttggttt
     4621 tgccggtttt aaagtgcttt acccgatcaa cagcaaagat aaaaacgatg aaatcgtcag
     4681 catgctcggg gccagctatt tccgcgtgat tggtgcaggt caggtttatg gcctttctgc
     4741 ccgcggccta gcaattgata ccgctttgcc atcgggtgaa gaatttccac gcttcaaaga
     4801 gttctggatc gagcgtccaa aaccgactga taaacgttta accatttatg cattgcttga
     4861 ctcgccgcgt gcgacaggtg cttacaaatt cgtggttatg ccagggcgtg acacggttgt
     4921 ggatgtgcag tcgaaaatct atctgcgcga taaagtcggc aaactggggg ttgcaccgtt
     4981 aaccagtatg ttcctgtttg ggccgaacca accgtcgcct gcaaataact atcgtccgga
     5041 gttgcacgac tctaacggtc tgtctatcca tgctggtaat ggcgaatgga tctggcgtcc
     5101 gttgaataac ccgaaacatt tagcggtcag cagcttctcc atggaaaacc cgcgaggctt
     5161 tggtctgttg caacgaggtc gtgatttctc ccgctttgaa gatctcgatg atcgttacga
     5221 tctccgtcca agcgcatggg tgactccgaa aggggagtgg ggcaaaggca gcgttgagct
     5281 ggtggaaatt ccaaccaacg atgaaaccaa cgataacatc gtcgcttact ggacgccgga
     5341 tcagctgccg gagccgggta aagagatgaa ctttaaatac accatcacct tcagccgtga
     5401 tgaagacaaa ctgcatgcgc cagataacgc atgggtgcaa caaacgcgtc gttcaacggg
     5461 ggatgtgaag cagtcgaacc tgattcgcca gcctgacggt actatcgcct ttgtggtcga
     5521 ttttaccggc gcagagatga aaaaactgcc agaggatacc ccggtcacag cgcaaaccag
     5581 cattggtgat aatggtgaga tagttgaaag cacggtgcgc tataacccgg ttaccaaagg
     5641 ctggcgtctg gtgatgcgtg tgaaagtgaa agatgccaag aaaatcactg aaatgcgtgc
     5701 tgcgctggtg aatgccgatc agacgttgag tgaaacctgg agctaccagt tacctgccaa
     5761 tgaataagac aactgagtac attgacgcaa tgcccatcgc cgcaagcgag aaagcagcat
     5821 tgccgaagac tgatatccgc gccgttcatc aggcgctgga tgccgaacac cgcacctggg
     5881 cgcgggagga tgattccccg caaggctcgg taaaggcgcg tctggaacaa gcctggccag
     5941 attcacttgc tgatggacag ttaattaaag acgacgaagg gcgcgatcag cttaaggcga
     6001 tgccagaagc aaaacgctcc tcgatgtttc ccgacccgtg gcgtaccaac ccggtaggcc
     6061 gtttctggga tcgcctgcgt ggacgcgatg tcacgccgcg ctatctggct cgtttgacca
     6121 aagaagagca ggagagcgag caaaagtggc gtaccgttgg taccatccgc cgttacattc
     6181 tgttgatcct gacgctcgcg caaactgttg tcgcgacctg gtatatgaag accattcttc
     6241 cttatcaggg ttgggcgctg attaatccta tggatatggt tggtcaggat ttgtgggttt
     6301 cctttatgca gcttctgcct tatatgctgc aaaccggtat cctgatcctt tttgcggtac
     6361 tgttctgttg ggtgtccgcc ggattctgga cggcgttgat gggcttcctg caactgctta
     6421 ttggtcgcga taaatacagt atatctgcgt caacagttgg cgatgaacca ttaaacccgg
     6481 agcatcgcac ggcgttgatc atgcctatct gtaacgaaga cgtgaaccgt gtttttgctg
     6541 gcttgcgtgc aacgtgggaa tcagtaaaag ccaccgggaa tgccaaacac tttgatgtct
     6601 acattctgag tgacagttat aacccggata tctgcgtcgc agagcaaaaa gcctggatgg
     6661 agcttatcgc tgaagtcggt ggcgaaggtc agattttcta tcgccgccgc cgtcgccgcg
     6721 tgaagcgtaa aagcggtaat atcgatgact tctgccgtcg ctggggcagc cagtacagct
     6781 acatggtggt gctggatgct gactcggtaa tgaccggtga ttgtttatgc ggcctggtgc
     6841 gcttgatgga agccaacccg aacgccggga tcattcagtc gtcgccgaaa gcgtccggta
     6901 tggatacgct gtatgcgcgc tgtcagcagt tcgcgacccg cgtgtatggg ccactgttta
     6961 cagccggttt gcacttctgg caacttggcg agtcgcacta ctggggacat aacgcgatta
     7021 tccgcgtgaa accatttatc gagcactgcg cactggctcc gctgccgggc gaaggttcct
     7081 ttgccggttc aatcctgtca catgacttcg tagaagcggc gttgatgcgc cgtgcaggtt
     7141 ggggggtctg gattgcttac gatctcccgg gttcttatga agaattgccg cctaacttgc
     7201 ttgatgagct aaaacgtgac cgccgctggt gccacggtaa cctgatgaac ttccgtctgt
     7261 tcctggttaa gggtatgcac ccggttcacc gtgcggtgtt cctgacgggc gtgatgtctt
     7321 atctctccgc tccgctgtgg tttatgttcc tcgcgctctc tactgcattg caggtagtgc
     7381 atgcgttgac cgaaccgcaa tacttcctgc aaccacggca gttgttcccg gtgtggccgc
     7441 agtggcgtcc tgagctggcg attgcacttt ttgcttcgac catggtgctg ttgttcctgc
     7501 cgaagttatt gagcattttg cttatctggt gcaaaggaac gaaagaatac ggcggcttct
     7561 ggcgcgttac attatcgttg ctgctggaag tgctgttttc cgtgctgctg gctccggtac
     7621 gcatgctgtt ccatacggtc ttcgttgtca gcgcgttcct tggctgggaa gtggtgtgga
     7681 attcaccgca gcgtgatgat gactccactt catggggcga agcgttcaaa cgccacggct
     7741 cacagctgct gttggggtta gtgtgggctg ttgggatggc gtggctggat ctgcgtttcc
     7801 tgttctggct ggcaccgatt gtcttctcgt tgatcctgtc accgtttgtt tcggtgattt
     7861 ccagccgtgc caccgttggt ctgcgcacca aacgctggaa actgttcctg atcccggaag
     7921 agtattcacc gccgcaggtg ctggttgata ccgatcggtt ccttgagatg aatcgccaac
     7981 gctcccttga cgatggcttt atgcacgcag tgtttaaccc gtcatttaac gctctggcaa
     8041 ccgcaatggc gacagcgcgt caccgcgcca gtaaggtgct ggaaatcgcc cgtgaccgcc
     8101 acgttgaaca ggcgcttaac gagacgccag agaagctgaa tcgcgatcgt cgcctggtgc
     8161 tgctaagcga tccggtgacg atggcccgtc tgcatttccg cgtctggaat tcccctgaaa
     8221 gatattcttc atgggtgagt tattacgaag ggataaagct caatccactg gcattgcgta
     8281 aaccggatgc ggcttcgcaa taaaaacgta
//
LOCUS       DAJTRH010000144         8234 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTRH010000144 DAJTRH010000000
VERSION     DAJTRH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..315
                     /locus_tag="OO339_003651"
     CDS             <1..315
                     /locus_tag="OO339_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5928132.1"
                     /translation="HLKLLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLA
                     MALSSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTT
                     TE"
     gene            287..2197
                     /locus_tag="OO339_003652"
     CDS             287..2197
                     /locus_tag="OO339_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5928133.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2198..2401
                     /locus_tag="OO339_003653"
     CDS             2198..2401
                     /locus_tag="OO339_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5928134.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2398..3991
                     /locus_tag="OO339_003654"
                     /pseudo
     CDS             2398..3991
                     /locus_tag="OO339_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3981..5486
                     /locus_tag="OO339_003655"
     CDS             3981..5486
                     /locus_tag="OO339_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5928135.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5523..5870
                     /locus_tag="OO339_003656"
     CDS             5523..5870
                     /locus_tag="OO339_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5928136.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5928..6956
                     /locus_tag="OO339_003657"
     CDS             5928..6956
                     /locus_tag="OO339_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5928137.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7008..7394
                     /locus_tag="OO339_003658"
     CDS             7008..7394
                     /locus_tag="OO339_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5928138.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7406..7783
                     /locus_tag="OO339_003659"
     CDS             7406..7783
                     /locus_tag="OO339_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5928139.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7770..>8234
                     /locus_tag="OO339_003660"
     CDS             7770..>8234
                     /locus_tag="OO339_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5928140.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1910 a         1935 c         2541 g         1848 t
ORIGIN      
        1 cacctcaaac tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag
       61 ttaaaaaatc aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt
      121 agtaagctgg cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga
      181 cagttcccgg atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga
      241 gcgaatcagt gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga
      301 acaacaactg agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt
      361 attgccggtg acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc
      421 atacggtgag ggggaatgga aaacgctgca gtttcaggtc gccatcatga actgcatggg
      481 caatgacgag attcgtacag ttaacctgat taaatctgcc cgtgttggtt atacaaaaat
      541 ggtgcttggt gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca
      601 gccgacggac tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga
      661 tgttccttgc ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct
      721 cacactgaag cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa
      781 aaactaccgt gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc
      841 ggatgtggaa aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt
      901 atggccaaaa tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa
      961 agctgcgaat gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga
     1021 ggagcagtat ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg
     1081 taaaccggaa acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga
     1141 acttgatcag accggggggc gctggatttg tgacaatacc gggatgtgga cgtgtgacgg
     1201 tctgacgttt ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca
     1261 tatatggacg gcgtacagtc cgttcaccac cagggtacag atagtctgtg actggctgga
     1321 tgcactgaaa gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg
     1381 ggaagaggcc gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta
     1441 cacggcaacg gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa
     1501 ccgttttgag atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa
     1561 aatcatcatc atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat
     1621 caataaaaaa taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga
     1681 tatcgggggg atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtattttccg
     1741 ggtactgccg gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac
     1801 ccgcaatcag cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct
     1861 ctatgcccga atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg
     1921 ctttcctgat aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga
     1981 actggtggaa aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagaggcg
     2041 taacgaagcg ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg
     2101 ctggcaactt gatctggctg tgcagttgca tcccgcaagg atgcaactgc acaaccgacc
     2161 cttgaagaac tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca
     2221 ggcgctgcgt caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa
     2281 agacggtcgc aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgattaa
     2341 cgatgcggag tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg
     2401 aaacgaacgc ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac
     2461 aacggaggcg gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt
     2521 gccgatgcgg ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg
     2581 cgcaataacg gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac
     2641 atgtttctta tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca
     2701 aaaagttttg tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc
     2761 gagatcgacg tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg
     2821 catgccttta acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta
     2881 ttccgtacgc gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg
     2941 gggaaccact ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac
     3001 catatctgtg aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt
     3061 gagcttccca ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag
     3121 acccgtgggg ctaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg
     3181 caggccacgc agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt
     3241 gaacttgata ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat
     3301 aatccgctta ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg
     3361 ctgggcggtg ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc
     3421 gcaggattca gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc
     3481 cggtcttggc gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag
     3541 tgcccgcgcc tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc
     3601 ggcccggctg gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat
     3661 tattcgtccg ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc
     3721 agagtggatt agctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt
     3781 gatgcgcatt gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga
     3841 ttatcaggac attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact
     3901 ctcacgtccg gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc
     3961 ggaagaggag acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca
     4021 atgagccgct gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg
     4081 agatgggcgc agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga
     4141 tgctggctga aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg
     4201 tggtgaacgg tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca
     4261 tgcggccatt ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa
     4321 tggcggacac tcaggtacgg ggcgtactgc tggacattga cagttctggc gggcaggcca
     4381 ccggcgcgtt tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg
     4441 cactgtgcaa tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac
     4501 ggctggttac ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct
     4561 atgccggtca tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca
     4621 aggtggatgg caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc
     4681 gcattgatgc ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg
     4741 tggatgcggt tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg
     4801 aactggcgga tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga
     4861 acacacacga tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg
     4921 agaaccagcg agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg
     4981 ccacgatgct ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg
     5041 tggcggcacc gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat
     5101 gtgaagaggc gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga
     5161 cggtggaaaa agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac
     5221 ttcgtgatca gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac
     5281 tggcggcctg tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag
     5341 gcagggcaga accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc
     5401 attcaccggc agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg
     5461 tgaaaatgct catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca
     5521 atatggtaac gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg
     5581 atccggccca taccgccaca ggctgcagcg ggatttcctc ggcaacaccg gctctgacgc
     5641 ccctgatgct ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc
     5701 atgtggttgg catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca
     5761 agtcggggac ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa
     5821 aggcgaatgc ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca
     5881 ggtcactgcg gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta
     5941 cgacccgcca gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg
     6001 agctgttttt ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta
     6061 ccggaaaaac gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc
     6121 accgtggcgg tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta
     6181 attaccagca ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacggcccgg
     6241 cttaccgtcg cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc
     6301 aggtggaaga gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag
     6361 agcagtttga aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg
     6421 caggagggac ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg
     6481 atgcgtactg tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct
     6541 ggcgtctgct gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa
     6601 attcacagct ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt
     6661 atggtgatct ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa
     6721 aacgttatct gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt
     6781 gctacggtgc cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt
     6841 atccgaaaca ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg
     6901 cgccgctgat ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg
     6961 tgagcgggag ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag
     7021 aagaaaacct gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct
     7081 ccggtagtgc ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg
     7141 cgggggaggt cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata
     7201 aaggcgagtc agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta
     7261 cggtggtggc actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac
     7321 cgggaacgtc gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg
     7381 caaaaaggca gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct
     7441 tgccggtgtc aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg
     7501 agaacttgag gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt
     7561 tgccgggggg atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat
     7621 atcgcagtta cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg
     7681 tattactccg gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc
     7741 acagaataat gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc
     7801 tgaacagcct cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg
     7861 cggcaaagat tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata
     7921 accaggtgaa aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca
     7981 gcccctcagg aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta
     8041 agctgggtac cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc
     8101 gtggcagcgt actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg
     8161 cgaatggtcg ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattgatg
     8221 tggtgaaaat cccg
//
LOCUS       DAJTRH010000145         8145 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTRH010000145 DAJTRH010000000
VERSION     DAJTRH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..315)
                     /locus_tag="OO339_003661"
     CDS             complement(<1..315)
                     /locus_tag="OO339_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5928141.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLS"
     gene            complement(402..763)
                     /locus_tag="OO339_003662"
                     /pseudo
     CDS             complement(402..763)
                     /locus_tag="OO339_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1102..1656)
                     /locus_tag="OO339_003663"
                     /pseudo
     CDS             complement(1102..1656)
                     /locus_tag="OO339_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1799..1921
                     /locus_tag="OO339_003664"
                     /pseudo
     CDS             1799..1921
                     /locus_tag="OO339_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2183..2749)
                     /gene="ipgB2"
                     /locus_tag="OO339_003665"
     CDS             complement(2183..2749)
                     /gene="ipgB2"
                     /locus_tag="OO339_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5928142.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3031..3345)
                     /locus_tag="OO339_003666"
     CDS             complement(3031..3345)
                     /locus_tag="OO339_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928143.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3423..3533)
                     /locus_tag="OO339_003667"
                     /pseudo
     CDS             complement(3423..3533)
                     /locus_tag="OO339_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3857..4534)
                     /gene="ospD1"
                     /locus_tag="OO339_003668"
     CDS             complement(3857..4534)
                     /gene="ospD1"
                     /locus_tag="OO339_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5928144.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4906..5043)
                     /locus_tag="OO339_003669"
                     /pseudo
     CDS             complement(4906..5043)
                     /locus_tag="OO339_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5125..5423)
                     /locus_tag="OO339_003670"
                     /pseudo
     CDS             complement(5125..5423)
                     /locus_tag="OO339_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6012..6171)
                     /locus_tag="OO339_003671"
                     /pseudo
     CDS             complement(6012..6171)
                     /locus_tag="OO339_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6175..7404)
                     /locus_tag="OO339_003672"
                     /pseudo
     CDS             complement(6175..7404)
                     /locus_tag="OO339_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7424..7771)
                     /gene="tnpB"
                     /locus_tag="OO339_003673"
     CDS             complement(7424..7771)
                     /gene="tnpB"
                     /locus_tag="OO339_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5928145.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7771..>8145)
                     /locus_tag="OO339_003674"
     CDS             complement(7771..>8145)
                     /locus_tag="OO339_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5928146.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
BASE COUNT         2085 a         1902 c         1792 g         2366 t
ORIGIN      
        1 cgagagagaa cgaatgaatg ccctgagaat ggtcattccg ccacgatatc cctggtctct
       61 gatctcgcga gcgattaccg ttgccgggat tttgtaagga tgagcatcgg cgatgcgttg
      121 acgaatataa tcccggtatt catccaggag tgaagcaaca gcaggtcgcg gcgtatattt
      181 tggcggctca gattttgcct gcaaataacg tttaacggta ttgcgggaga gccccagttc
      241 tctggcaatc gcccggctac tcattccctg cttgtgcagg attttaattt ccataactgt
      301 ctcaaaagtg accataagct ctcctgaatc aggagagcag attaccccct ggatctgatt
      361 tcaggcgttg ggtgtggatc actattgcac cgttcgtgac agtgttgttg cgccttgctg
      421 ccaacgaagc tccactgccc atcaagttca cagataaggg caacatcagc gtgagcgact
      481 ggcgcagatg ttatccgccg gggcgggagc tttttaatgt ccgtatgacg gtattgatgc
      541 cgattttcag cgtacggcca gtatcgcgaa ctccggcacc gttgaaagcc atttcagtga
      601 tttggttttt aactcccggt ttacgggcct cgtaacagta agtgagctga aacacccggt
      661 gacagtcacg gcaacggaat cgatcatgtc cttttgggtt ctgcccgtga cggtaaacct
      721 gaacggactg gcaacggggg caatgaaagt taactgtgac catgaaagaa cctaaaagcg
      781 ggtattataa cccctaattc aactaattgg aggcattgtc atttttaatc tatggataca
      841 cttaaaaatc ctctcacctt acgttaaaaa ccttctcatg tttagagttt tttattatgt
      901 ttatcataat gatatgacga tctgagtttt taaaatcaag aagaaaagat aagtttttta
      961 aatgcacagt gcctgtggat aactttgcgt aaatccggag gacagatcac tctgaagcaa
     1021 gacaacagca tctgaaaaca atatgctcac gtttcaacca cagggcgtaa gtgatcctac
     1081 ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt ccggactgag
     1141 gccgccacac caactgtgcc gtcgccagcg attgtaatca cattcgatat aattaaacac
     1201 tgttgccctc attatttccc ggctgataaa gtgttctccg tggagacatt ccactttcag
     1261 cgaatgaaag aagctttcca cgcaggcatt atcgtagcag cagccctttg cgctcatact
     1321 gccacgcaga ttatgccact tcagcagcgc ctgataatct gctgaacagt actgacttcc
     1381 tcggtccgag tgaacgataa cgttccgggg tctcttacgc cgccacagcg ccatctgcag
     1441 ggcatcgcag gccagttgcg ccgtcaggcg tggtgacatg gaccagccaa taacggcacg
     1501 tgaccacagg tcaatgacca cagccagata cagccagcct tcatctgtac gtaagtacgt
     1561 gatgtctccc gcccacttct ggttcggacc actggcgtaa aaatcctgct ccagcagatt
     1621 ttctgacaca ggcaggccat gtgcgcggta actgaccgta ttgctgggta agatcaattt
     1681 gctcactggt atttactacg tgcatcgaat gagtgatcaa gagccgtatg acgcgagagt
     1741 gtcacgtacg gttccgtaag cagccggaga gtgaaactct ctccggctga ctcgactcgc
     1801 aggttccgac agcggtgacg aacatgcagc ggtgttgttc tcgctgatcg gcacatgccg
     1861 tctgaacaat gtggagccag aaaaatggct gcgttacgtt attgaacata tccaggactg
     1921 accggcaaac cgggtacgcg atctgttgcc ttggaaagtt gatctgactt ctcagtaaat
     1981 atcaatatga ttctgacgag ccgcttacct ttgagcagag aaaggggaga gggatcccac
     2041 atccatgagc taggaaaata caaaaactgg ctatacaaca attcaatata ttaggactta
     2101 cttgcgagga gaaatatagc accgctcagg caattcctgg gtatttactt ttcttagttt
     2161 ctttactcgg gatgttctgt catcagaaag gcgattctaa atttgtaata tagtcactca
     2221 tattcccacg taatttcttt agatcaatat attgcacatt tttctcaaaa aactgatcgg
     2281 aaataagatt tgcaacattg cttgttgtat catttttaac gcttctattc atcccttcac
     2341 aaagttcgtc cattttcttt ttgaaataac tgcttcctga tacaagatga tttatggaac
     2401 tttgtgcagc attgatgtct agagaaatgc gataatcttt ctcaacctgt ttaaatatct
     2461 gtttacggag attatcgccg atttttcggc tgtgttctgc acaaaactta tcaatacatt
     2521 ggtttaccac ccgggatacg tagacttttt tttcatcatt tttccaatct gtaatatttt
     2581 caccgatttg ttctgaaaca gagctcaaaa tactagtatt tattttggtt gatgatattt
     2641 ttactatcct tttccccatt gtacagcgga taatctcatc cacctttcct gaaaaatatc
     2701 ttttgtgaga taaactgatt ccaaaattat taaaagatgt tccaagcatt ataatcacct
     2761 tgtcatgtaa aatagtaaca ttgtcattat tttagctgat tcaaacaatt ctgtaaatgc
     2821 tttgagttct ttactccctt ggctcaatga tggaccgtct ggaatcccag agactaaact
     2881 ctctgagaaa gagagtatga aaaaacttgg ggcagtccct aaatgaaggg accaacagcg
     2941 gtaaaataac cggcatcatt cataatgatg ccctgttagt tgtagttatg aagatttacg
     3001 tcattaaaat tgctgttcgt agtgtcagtc ctatggtctg gcatcagctg aggatcgtag
     3061 ccgatacatc actggcttca cgacattcgt gttgaggcta ttcatgaaaa ctccacgctg
     3121 aaaacgccgt tttgtataaa cggtcacggt atgcccggag ccactgttgc tgataaattc
     3181 gataaaacct tggcgtttct tgaagctatc gttaatgcgg ataatgaaac aacgattggt
     3241 gagattcgct cttttgccga cgccctggac gctgtccggt tcaatcgcaa caaaatcaac
     3301 cggcagctga gcaaacccaa ccttgcctct ctggcgctgg agcatgaagt gatttggctg
     3361 ggacgtagcc gctgacagct tctataaaag tatgatccta cccacgtaat atgaacacag
     3421 gcttaagtga ggttctggtt ttcaaattgt tcaggactga gacctccaca ccaactgtgc
     3481 cgtcgtcagc gattgtaatc gcacttgata taattaaaca ctgtatattt catttgtgga
     3541 gtctccagtc tgagagggct ttatgcattc cgtattgggc actatgatgc cagaaatctg
     3601 caaggctcca caccttcctg tatttccctt cctctatcac agtgcacttt acttaatagg
     3661 gtgggatgtg ttcattacat ttattgtgtt ttttatgatt gtttttgcgt ccagtgtcgt
     3721 tcgtcatggg aaactggttc tatgggtagt atcgctcagt ctagttaact gtttgtttct
     3781 ttttagaaat taagcagatc acacaattcg gggagagatc cttctaagtg caattctttg
     3841 cagctattta gcacacttaa aacaactctt gtattttgtc agaaataata ttaaacatgt
     3901 cttgttcatt attgttaagt ctgtctgcta tttctgtaag ttttatccca ctatgatggc
     3961 atatatttat atatgccatc atagcctcga tattcccttt ccctgcggcc agaaataatc
     4021 ctggtaaccc ctcattatct cttacatcca atagtttaat cgtttctttt ggtgataaat
     4081 caaaagtttt aataaaatct ccataagctt gaatcgcagc ggaatgacct tcttgaagag
     4141 ctatgaacaa agcagattcc ccagcattat ttcgtgctgc aagaacttct agtttttcct
     4201 ctttgggtat ttcggctgcc aacagcgatt caccgtattt acgcgctgca tcggcatttc
     4261 catgccaaat caatgaaaac aaattatggc ggttatactc cttattattc tttgtcggaa
     4321 tggatatgtt ctgtattttg gccaattttc tgaaagaaac accactatat cccccccctg
     4381 atttttcttc attgatggcg tgggatatag cggtatttgt cacgttaatg atattatttt
     4441 tcattccttt attttcatta gcagtattgc tgcctattat taagtgcata tttttgttgt
     4501 ttgctggatg taatccatag ttatttattg acatttttgt tcatcctttt tgctaaaaaa
     4561 aaacaataac ctatttttgt aatgtaatag atattagtct attgctaatc taaaaaaacc
     4621 attgccacat tgaatggcta tattcaccaa taaattcttt cgatttcgta tattgacata
     4681 tcgtagatat atcttatccc taagattcaa taatcgagtc cgtttaccat tctgtgcaag
     4741 cgccttcact cagaagtgac cgtcaaggcg atcaccaaac ttgataataa agcagctcat
     4801 tgccatacgc cagtccttaa ggggcgttgt ccatttctgt gatgctgcct agatttaact
     4861 gccagctccg gcttatctac atctaaatat gtgagcctaa taccgctaca ctttgtccag
     4921 actatgtttt tctccgggaa gtgggctaac cgtctccata aagtgacgaa cttttttcaa
     4981 cagtggccat attgaacggc actgatgatt acgggttatt gtctcatgaa gagccggcca
     5041 catcactgac agcagtgact tcaggccttc agcaccagac agcctgaacc agttaatcca
     5101 tcgcccgaca aatttagtaa tctagggctg gtttttcttc agctgttgcg gttcgaaagt
     5161 gccattacga tcacgcagag tacggatttc cagaggttca tctccagtag tgataatctt
     5221 cgttaagcaa ccattgcggg aattgctgcc agatctaagc tgatttttct cgcacccaag
     5281 atgatgagtc atattagcat tgagtacggt ttcattttca gcatccaatc aaactgactg
     5341 agatcttcag gatttttgag acttttggct agttcattag ctaaagcccg caacttatta
     5401 gatgtcaact gtttttcatt cataaattca ccttcatttg atatcaaatt aaatagttca
     5461 acatcagaga attatacaaa cttatgtaca ggctcctatg atcttcgaat cctgaagatt
     5521 atagtgaaaa taagttttca cgttgaaatc ccacatagtt agtatatgat aaataatcat
     5581 ggatgacctg attaacctct tggggtagtg cttttataga agaataaata ttctactaat
     5641 ttaatacatc acctaaaata gtattgtttt ttatggttgg ttgaaggtcg tggtgatgat
     5701 gttagtcctt ttgagaatat tccagtgcca gatcttactt ataataaagg ggcttggcag
     5761 tttggataaa ctattgttct tattccacta atacaaactt tttgtaaagt atattaaata
     5821 tacttcattt catactgaaa tccccggaag tattcaatca aacaattaca ccataaaagg
     5881 aagagggggc cctaattaag ttgtcaagca tgtttatgat acctgcaggg tataaatttt
     5941 ctggctgcaa gcagacacat ataaaacgtc tttattgatc ctacccacat aatatggaca
     6001 cagtcgtaag tgaggctctg gttttcaaat tgttccggac tgagaccacc acaccaacgg
     6061 tgccgtcgcc agcgattgta atcgcactcg atataactaa acactgttgc ccgcactatt
     6121 tcccgatgga taatagtgtt ctccgtggag acattccatt ttcagcgaat gaattttcat
     6181 ctgttgctgt atccagtcat acagtgactg catcagtggc gcggctctgg cttttcttgc
     6241 cgccagacgc tgttctgctg aacatccccg gacttctgcc tcgatggcat acagttcacc
     6301 gatacgctgc agggcttccg tggtgatgtc ggttggcact cttgcatgca catcgtggat
     6361 ttttctccgg acatgagcca tacatgcggc ttccgttatt ctgccggatt cgtacaacgc
     6421 ccggtaacca ccgtaagcat cggcctgaag cactccgctg taaccggcca gatgattctg
     6481 tggatggata cctttccggt ccggtgagta cgcgaaccag accgccggag gcatttccga
     6541 accggcgtta cggtcatcac gaacgtagac ccacagccgg gcagtccttg ttttaccgct
     6601 gcccggctcc tggaccggaa cggggatatc atcggcatgg actttaccgg gcatcagcac
     6661 atactggcgc aggatgtcat acagcggttc cagcagttca gcaacagcac ctgtccagcg
     6721 ccccagtgtg gcacggctca gctccacgcc ctgacgacgg tatatttctg actggcggta
     6781 taacggcaga tagtctgcat attttccagc aacaacatgg gccagaagcc ccgctccggc
     6841 ataactgcgt gcaatgggtt ttgaaggcac cggtgcctgc acgatatggt cacaccggca
     6901 acaggccagt ttcggacgtt gtgtttcgat aaccttaaag gcgctgctga taagctccag
     6961 ctgctctgac acatcacatc ccagagaact gagttcgcca ccacaggccg ggcagcattc
     7021 ctcttccgga cggatgaccc gggtttcacg gggaagtgag gctggtaacg gtttacgggc
     7081 tgaagactgg cgcaggggag atggcagtac cgggtcatat tgctcaccca gcgtttccgc
     7141 catttcttcc tgaagtgcgc tgattcgctc ctgtgcttcc agtatctgcc gttcggtttt
     7201 tgcgcgaagt ttttctgagc ttttaccgaa ctgcatgcgc tgcagtttcg caaccagcgc
     7261 cttcagccgg ttgatttcgg aagcataagc cgccacccgc tgtgagagca ggcggttgta
     7321 tttcgccatc tggcggatgg tgtcctgttg cgtctgcaac agtgcccgca ggcgggcatt
     7381 ctcatgagca agtgaggtgt ccatatcctc actttacaac gggttatatg cggatgccag
     7441 cgcgttccgt tcgtttcggg tgtttccagt cgatgccctc aagaagcatg gataactggg
     7501 ccggagtaag gtgcaccttg ccgtcacggg tgaccggcca gacgaagcgg ccccgctcca
     7561 ggcgtctggt aaagaggcac agtccgtcac tgtcagccca caacactttt atctggtcac
     7621 cccggcgtcc gcggaagatg aacagatgcc cggagaacgg gtcatccttc aggacgttct
     7681 gaacttttga gaccaggccg ttaaagccat ttcgcatatc ggtgatacct gcaaccagcc
     7741 agatacgcga acctgcaggg aaagatatca tcagtggctg ctccctttta tttcgcggat
     7801 aagtatctgt aataacgccg gcgtcagttt acctttaagc ctgagagttc cggccggcag
     7861 aaccagctca cagcacagac tgtcggacgg tgtatttatc tgctctggtt cctgtgccgg
     7921 agccgggatt ttattatccg gctccggcgt taacgtcacg ggaagcagtg caggcatatt
     7981 ttttccggaa ggcagcaggc cacctttccg gtattgatgg cgccagttga agagcaggtt
     8041 atcgttgatt ccgttttcac gggcgatctg cgccacacag gctccgggct gcagtgactg
     8101 ctccactaaa gcgattttaa attcataagg aaaattaggc cgccg
//
LOCUS       DAJTRH010000146         8092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTRH010000146 DAJTRH010000000
VERSION     DAJTRH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..1136)
                     /gene="hprS"
                     /locus_tag="OO339_003675"
     CDS             complement(<1..1136)
                     /gene="hprS"
                     /locus_tag="OO339_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5928147.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDA"
     gene            complement(1136..1807)
                     /gene="hprR"
                     /locus_tag="OO339_003676"
     CDS             complement(1136..1807)
                     /gene="hprR"
                     /locus_tag="OO339_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5928148.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1940..2353
                     /gene="hiuH"
                     /locus_tag="OO339_003677"
     CDS             1940..2353
                     /gene="hiuH"
                     /locus_tag="OO339_003677"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5928149.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2462..3466
                     /gene="msrP"
                     /locus_tag="OO339_003678"
                     /pseudo
     CDS             2462..3466
                     /gene="msrP"
                     /locus_tag="OO339_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3467..4102
                     /gene="msrQ"
                     /locus_tag="OO339_003679"
     CDS             3467..4102
                     /gene="msrQ"
                     /locus_tag="OO339_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5928150.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4360..5010
                     /gene="zinT"
                     /locus_tag="OO339_003680"
     CDS             4360..5010
                     /gene="zinT"
                     /locus_tag="OO339_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5928151.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(6088..6357)
                     /locus_tag="OO339_003681"
     CDS             complement(6088..6357)
                     /locus_tag="OO339_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5928152.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            6414..7082
                     /locus_tag="OO339_003682"
     CDS             6414..7082
                     /locus_tag="OO339_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5928153.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            7398..>8092
                     /locus_tag="OO339_003683"
     CDS             7398..>8092
                     /locus_tag="OO339_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5928154.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2346 a         1804 c         1621 g         2321 t
ORIGIN      
        1 ttggcatcaa gaaaactggt tatatgaata cgcgattttt ctggtgaata tcgaatggcg
       61 ttaacaataa gattcgataa cattcgttgc agtaaaattt tatccgcaaa gatttgttga
      121 ttgcacttga ccttaaagca aatctctttc tcgtctgaaa gatattcaag atagtccaaa
      181 agattttcaa cttccttatt gagagaaagc gaatccagtt tcaccaaaac attgtttttg
      241 tctgccctgg caagaaacag tatattctct gttaaccgcg aaatattttc cagctcttca
      301 atgtttccgg caattgtttt ttgatattca gcgatacttc tgatttgact gagcgtaacc
      361 tgattctgac ccagtaatgc attaattggc gttctaagtt catgagcgag atcgtcagca
      421 aattgactaa ggcgctcaaa atctttgact aaagcatgat gcattttatt caacgcctgc
      481 ccaagaggtt ttagttctct cggtaatgcg ttaacctcaa ccggcactcg gctatcgtta
      541 taattcagcg cttccgttac gccactcaac tttttgatct ctcgcaaccc cgtcctgatt
      601 aacaacggac ttaatactga gcacagtata atggcgacaa tgcaaattat aatgctatta
      661 attttatact gttcaagcat attatgtctg gctgaagcca atttagccac agtaaccgtt
      721 aatgatggcg aaacttcatc aatgttaatt cgtaaagcat ctatctctgt atcattaata
      781 atgcttctgt aaatgcctgc tgcgctgatt gtctcactag caggtatgtt atttaacatg
      841 tcatcactga catttgtccg gttaacaatt ttattgatgc catcactatg aatgatcaaa
      901 atatcctgac taacatccat catccggtta aagtacacag gtaacgtatc tggatttacc
      961 ccatcaatta acaactgctt gatctgcgct gtccggttaa tgagtgttgt atcatcgcgc
     1021 catttcaact cacttgccag gccattatag agagtccata caattccagc gccagcaaca
     1081 gactgcagaa atataaaaag caaggttaaa cggacggtta tagataatct tttcattatt
     1141 tttttaccgc tacgaatgaa tagcccatcc cccggattgt ggcgattagc ttttcaggaa
     1201 aaggatcatc aacttttgcg cggagcctgc gaatggcgac atccaccgta ttggtatcac
     1261 tatcaaagtt gattccccaa atttcactcg caataaccgt tctgggtata atctcgccag
     1321 ctctggaggc cagtagccaa agtaactgaa actccttgcg cgtcagtgta atactgatat
     1381 tgtccctgct gacactttga ctgacagagt ccatttttaa gccactgatt tctaatgttg
     1441 aattcagagc gtgatgttgc cttaattgtg cccgaaccct tgctagcaac tcagaaaatg
     1501 aaaaaggttt taccagataa tcatttgccc cactgtccaa ccctctgact ctgtcatcga
     1561 cagaatccct tgcagtaagg caaataacag gggtttgctt tgccgttctt aacgtttgta
     1621 agatctgcca gccatccata cccggaagca taatatccag aatgatcaat gcataatcat
     1681 ccttcagcgc aagataaagc ccatctctgc catcagaaac ggcatcaatg acataacccg
     1741 cttcggaaag cccctgcgtt acccattcct gggtcctttg attatcttca ataagtagaa
     1801 tcttcatgaa aataatatgc cataaatcat atgttataga aacaacctgg ttcattacaa
     1861 aattgtaatg ctgctgtaag gttaccctgg acgttttttc gctatcctca aaactcattc
     1921 acatgacaag gatataaaca tgttaaagcg ttatttagta ctctcggtag caacggcagc
     1981 cttttcatta ccttctttgg tttatgccgc acaacaaaac attcttagcg tgcacatttt
     2041 gaaccagcaa acaggaaaac ccgctgccga cgtgacagtc actcttgaaa agaaagcgga
     2101 taacggctgg ttacaactta ataccgccaa aacagataag gatggacgaa ttaaggcact
     2161 gtggcccgag caaactgcga ctactggcga ttaccgtgtc gtatttaaaa ccggggacta
     2221 tttcaagaaa caaaatcttg aaagtttctt ccctgagatt cccgttgaat ttcatattaa
     2281 taaagtgaac gagcattatc atgtgccttt gttactcagc caatatgggt attcaaccta
     2341 tcgtggcagt taatgcgtaa atatgtaaaa gctgatctct gctactgtta gggaggtgac
     2401 tccctgacag attaacagta aacagctctt gttggttaac gacaaaaaag tgtgatggct
     2461 tatgaaaaag aagcaatttt taaaagaatc agatgttacg gccgagtcgg tattctttat
     2521 gaagcgtcgg caggtgttaa aagcactggg catcagcgca gctgcatttt ctttgcctca
     2581 cgctgcgcat gccgatctgc ttagctggtt taaagggaac gatcgtccgc ccgcccccgc
     2641 cggaaaaccg ctggagttca gcaagcctgc cgcctagcaa aataacctgc cactgacgcc
     2701 agcagataaa gtctccggtt ataacaactt ctatgaattc gggctggata aagacgatcc
     2761 cgcggctaat gctggtagcc tgaaaaccga tccatggaca ctgaaaatca gcggcgaagt
     2821 ggcaaaacca ttgaccctcg atcatgatga tttaacccgt cgcttcccgc tggaagagcg
     2881 tatttatcgt atgcgctgcg tggaagcgtg gtcgatggtg gtgccgtgga ttggttttcc
     2941 gctacacaaa ttgctggcgc ttgccgaacc caccagcaat gcgaagtatg tcgctttcga
     3001 aacgatttat gcgccggaac agatgcctgg ccagcaggac cgctttatcg gcggcgggct
     3061 gaaatatcct tatgtcgaag gattgcgtct cgacgaagca atgcatccgc tcacactgat
     3121 gaccgtaggt gtttatggca aggcgttacc accgcaaaat ggcgcgccgg tgcgactgat
     3181 tgtgccgtgg aaatatggct ttaaagggat taaatcgatc gtcagtatta agctgacccg
     3241 cgagcgtccg ccaaccacct ggaatctggc agcgcctgac gaatacggtt tttacgccaa
     3301 cgttaatccg catgttgatc acccgcgctg gtcacaggct accgaacgat ttattggttc
     3361 aggcggcatt ctcgatgttc agcgccagcc aacactactg tttaatggtt acgccgacca
     3421 ggtggcatcg ctgtatcgtg gcctggattt gcgggagaat ttctaaatgc gtctgacagc
     3481 aaaacaagtg atatggctga aagtttgcct gcatcttgcc ggattgttgc cgtttctctg
     3541 gctggtctgg gcgatcaatc acggtggact gggtgccgat ccggtgaaag atattcagca
     3601 ttttaccggt cgcactgcac tgaaattttt gcttgcggcc ttgttaatca cacctctggc
     3661 acgctacgca aaacagccgt tattgatacg cactcgccgc ctgttaggct tatggtgctt
     3721 tgcctgggcg acactgcatt taaccagtta cgcattgctg gagttgggcg tgaataatct
     3781 ggcgttatta ggtaaggagt taattacccg gccttattta acgttaggaa ttatcagctg
     3841 ggttattttg cttgctttag cgttcacttc aacccagtcg atgcagcgaa aactcggcaa
     3901 acactggcaa cagttgcata acttcgtcta tcttgtcgcg atcctggccc caatacatta
     3961 tctgtggtca gtgaagatta tttcaccgca gccactcatc tacgccgggc tggctgtact
     4021 gcttttagcc ttacggtata agaagttgct ttcccttttc aaccggttac gcaagcaggc
     4081 ccacaacaaa ttatctctat aacttcatat tgccgacaaa cttcgcgtta tcccggccgg
     4141 atgcggcgcg agcgccctat ccggcccaca cagatcgcat caagttatgt ttcgtttgtc
     4201 atcaaccttt catcaccaga ataatttatt ttatttcaat aagatagttc aatacacctg
     4261 agaataccat gctctcattt cctaatgagt tgttgcattt tttcatatgt tacaatataa
     4321 cattacacat aatgtacatt aactctggag gaaattgttt tggcgattcg tcttcataaa
     4381 ctggctgttg ctttaggtgt ctttattgtt agcgctcctg ccttttcgca tggtcatcat
     4441 tctcatggca aacccttaac agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat
     4501 accaatgtac aaaaccgaac gctcagtgac tgggatggag tctggcaatc cgtttaccct
     4561 ttactgcaaa gtggcaaact tgatcccgtc tttcagaaga aagcggatgc agataaaacc
     4621 aaaacatttg ctgaaattaa agattattat cgcaaaggtt atccgacaga tatcgaaatg
     4681 attggcattg aggacggcat tgtcgaattc catagaaata atgaaacaac atcctgtaaa
     4741 tatgattacg atggatacaa aatactcacc tataaatcag gcaagaaagg cgttcgttac
     4801 ttattcgaat gtaaagatcc tgaaagcaaa gcccctaaat atatacaatt tagcgatcat
     4861 ataattgcac cacgaaaatc atctcatttt cacattttta tgggtaatga ttcccagcaa
     4921 tctttattga atgaaatgga aaactggcca acgtattatc cctatcagtt gagtagcgaa
     4981 gaagtggtcg aggagatgat gtctcattga tttttttgcg ctgacttcag ttatttaaca
     5041 gaacagtgcc tggtagtaac aacaataatt taccaagaca aataagtaag acaaactcag
     5101 acacgctcat acttgagtcg tgtctttttt attatgtctg tagaaaaaaa ctgagaaaaa
     5161 ggctaacaat aaatacacaa ctcagccata cagactggca gcgcaaatgt ttccaggagc
     5221 aagtcatctg tcatggtaaa gctaaccaga aatataggtt aattttggtg tttaaacctt
     5281 ttttacttgt tagtatgata caaaggcttt caaaaaagct gggaaattca acaactcttg
     5341 gaaattatgg actttgtccc gcggctttaa tctgacggcc gcgttccttt tttggctcaa
     5401 ttattgaccc cttctctacg caacttcagt tcccgccagc aactttgcgg cagtttcata
     5461 ggatcaatgt ctaaaagaat aatggtgacc gataagaaaa cgactgaata actgtagatt
     5521 ttcgctcgaa accttcctgt cagctccata gcgaatcaag cgctgaatgt cacagtatcg
     5581 aacagaaaac agtgacgatc taacccttca agaatattct acgattgttc tgtttaggaa
     5641 aagcaaggcg ggaagtcggg agataagtca ttgataaaat ggcggagaga gggggatttg
     5701 aacccccggt agagttgccc ctactccggt tttcgagacc gatgatacag agtcgaaaaa
     5761 tcaaagagtt aaaaattttt cttggaaaaa atgtcttaaa atcgtacacg aaaaatcaat
     5821 gaattacata acattaaaga agaatattcc aagccttctt gagtccacca cctgttttca
     5881 ggtgtcattc agacgtgatt tgccccagat tgggaaagca cgcttttttc tcagcttatt
     5941 ccgttttgcc tgtgtgttga ataattccac tgcagttata taaaatcggt aacggctgga
     6001 aatcattcaa tactcgcact atcgaaagtt taccagccaa ccgcagcacg tcctgcatac
     6061 gtcgtgtctg cggtttttct ttttcgctta cattgtgtct ggttcttccg gccactcaat
     6121 atcaggtgca gttgatgtat caacacggtt cagcaacacc cgatacttct tccaggcttc
     6181 cagcaacgag ttttcttcct ccgttgcgat ctccagatct acagcatcct gaagtggcgc
     6241 aatatgctca ctgaatccct ggatgtagaa ctgtgtggtg acggtcttcc agccattcgg
     6301 ctcctgctgt atcgaagcat accaggctat ttcaatatcg ctatgctgcg gcagcattta
     6361 accccttgta attcatcgcc ataattgatt taattcacaa ataaaactat aatatggtga
     6421 aattaatgaa aaaaaacaca gatgatgggg ctaaaattta cacaccactt accctaaagc
     6481 tttatgactg gtgggttttg ggagtatcaa atcggcttgc atggggatgt cctacaaagg
     6541 aacaccttct tccacacttt ctggaacatt taggtaacaa ccatctggat attggtgttg
     6601 gaactgggtt ttaccttact cacgtacctg agagtagtct gatatcttta atggatttga
     6661 acgaagctag cctgaacgcg gcatctacaa gggctgggga atcaaaaatt aaacataaaa
     6721 ttagccatga tgtttttgaa ccttatcctg cggcgttaca tggtcaattt gattccattt
     6781 ccatgtttta ccttcttcac tgcctgcctg gaaatatatc tacaaaaagc tgtgtaatac
     6841 gcaatgcggc gcaggcctta actgatgatg gaactctata cggagccaca attcttggcg
     6901 atggagttgt gcacaatagc ttcggtcaaa aactgatgcg catttacaat cagaaaggca
     6961 tcttttcaaa cacaaaagat tccgaagaag gcttaacaca tatactctca gagcatttcg
     7021 agaatgttaa aaccaaggtt caaggtactg tagtaatgtt ttccgcttca gggaaaaaat
     7081 agcatccaac cgcagcacgt tcttgcttaa gacgtgctac ggtttccttt acgatttggg
     7141 gttgcgactt tacctctatt gataatgcat tccggcagaa cgttcaaata tgaacgtacg
     7201 atatttaact aaccgaaaaa caaaatatag cacaggcgag acatttacca tctaagaaaa
     7261 tagtatcgtt tttttacagt taaatcaaca tcacttcctt aaaatgaaaa caataaattt
     7321 caatcctgaa acattttatt tcagcttatt attccccagt atcacatttt atcaggatat
     7381 ccttctgcga ggttattatg cttcctgtaa ataatccccc cctatccact ggaaacgtct
     7441 ctttttacag aactacatca atcgacaatg ttcacaataa ttatctctcc gaatgggttg
     7501 aatggactaa aaacagcatt tccggagaaa acagggaaac tgcttttacc cggctccaat
     7561 tatgtctgga gaacagtgaa acatcgttgg acttatcttg tttaggtctc agatctctac
     7621 cacgattgcc tgacaatctt gatgaaatta atgtaagcaa taaccaacta tcaatgctcc
     7681 ccgagctacc aagggcattg aaagagctga atgcaagcag taatcaatta tctgcacttc
     7741 ctgaattacc agtgtcgctg gaatatataa atgtgagtga taaccatttg ttcgcacttc
     7801 ctgaattacc tgcgtcacta gaatatatta atgtaagtga caatcacctg tctgtacttc
     7861 cgaggttacc aatgtcattg gaattacttg atgcagccag aaatgctttg gaagtaatac
     7921 cagattttcc agaaagagat gatcatatta taagaatatt ctggcttaat cagaaccgga
     7981 tcacggcaat tccggaaagc atacttggcc tcagttctga tagcgttgtc aatcttagag
     8041 aaaatcaact atctcccaga ataatgcaaa ctttgttaca acaaaccgcc ca
//
LOCUS       DAJTRH010000147         8058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTRH010000147 DAJTRH010000000
VERSION     DAJTRH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OO339_003684"
                     /pseudo
     CDS             complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OO339_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            764..916
                     /locus_tag="OO339_003685"
                     /pseudo
     CDS             764..916
                     /locus_tag="OO339_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2297..3244
                     /gene="icsP"
                     /locus_tag="OO339_003686"
     CDS             2297..3244
                     /gene="icsP"
                     /locus_tag="OO339_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5928155.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3806..4178)
                     /locus_tag="OO339_003687"
                     /pseudo
     CDS             complement(3806..4178)
                     /locus_tag="OO339_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4422..4541
                     /locus_tag="OO339_003688"
                     /pseudo
     CDS             4422..4541
                     /locus_tag="OO339_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4791..5657
                     /gene="ospB"
                     /locus_tag="OO339_003689"
     CDS             4791..5657
                     /gene="ospB"
                     /locus_tag="OO339_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5928156.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5986..6726
                     /gene="phoN2"
                     /locus_tag="OO339_003690"
     CDS             5986..6726
                     /gene="phoN2"
                     /locus_tag="OO339_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5928157.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7086..>8058)
                     /gene="ospC4"
                     /locus_tag="OO339_003691"
     CDS             complement(7086..>8058)
                     /gene="ospC4"
                     /locus_tag="OO339_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5928158.1"
                     /translation="DKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLN
                     KTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVS
                     RYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRII
                     NFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYS
                     LGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFI
                     NNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2526 a         1502 c         1506 g         2524 t
ORIGIN      
        1 acaattaata tacattaagt aacaggcatt cgagccttga tttgagaaag cctgtcattc
       61 atttgtttta tgtagctatc tatttcctct gatgcataac taaaaagttc tcccatttca
      121 cgagcaataa taatctcaga tttaatagac tttaatctct ggcgactcat ctcttaacac
      181 ttgcataatc tctcttttta tatcatatag ttctataaac cgatttgccc gtagttctcc
      241 ttgggaagaa tcatttctga atacaacaat tatatacaaa aaatcactat tttctttaga
      301 gaagatctta caactaatct ctgggagctc acataatgca tgtaagtcag gatggtacaa
      361 actatccaca gaaaattgcc ccataggacg ttttccagat ataactgtca accagcttcc
      421 ttccggtgta ctggcttcgg ctctggaaat aggctgaaaa tcttccggac atggagaata
      481 cattgtcttc atataattaa gtcgtctgga tttctcatta ataaactcct ctatttttaa
      541 ttgtgccata cttctggtta taccagagat atacttcttg tcatatatgt ttggtgattc
      601 attaaagtaa ccatgttcaa ctctaggata aatatttctc actatatatt cagtatttgc
      661 agtatttatt ggaaatacgt tcttaatatt cttgatggga ctaatcattt taatctctat
      721 actcaaatat aaacattacc atgaacaata taattgttcc taagtggaat gtctccacgg
      781 agaacacttt atctatcggg aaatagtgcg ggcaacagtg tttaattata tcgagtgcaa
      841 ttacaatcgc tggcgacggc accgttggtg tggcggcctc agtccggaac aatttgaaaa
      901 ccagaacctc gcttaggcct gtgtccatat tacgtgggta ggctcacaga gcctcactta
      961 cgactgcgtc catattatgt gggtaggatc aataaagacg ttttatatgt gtctgcttgc
     1021 agccagaaaa tttataccct gcaggtatca taaacatgct tgataactta attggggctc
     1081 cctcttcctt ttatggtgta attgtttgat tgaatacttc cggggatttc agtatgaaat
     1141 gaagtatatt taatatactt tacaaaaagt ttgtattagt ggaataagga caatagctta
     1201 tccaaactgc caagcctctt tattataagt aagatctggc actggaatat tctcaaaagg
     1261 actaacatca tcaccacgac cttcaaccaa ccattttatt gcatttaaat cccagaatag
     1321 ttcagtagtg gtcaatttgg gaaacgcaca atattgtacg gttacgttat cgcaatagag
     1381 tgaactctcg ccctatacgg cgttttcaag gattagtcct taatcggaca accacattaa
     1441 agcataataa ataattaagc tttaataata tactatagat acagttctat tataaaaaat
     1501 actattttag gtgatgcatt aaattagtag aatatttatt cttttataaa agcaccaatg
     1561 aattgtccaa gtaaacatga gataaaatgt gtagaggata actgagtttg gtcactttaa
     1621 ctgtattagt cgcagtctga ctctcgactt taaaaggatg ggaattacct gtactgatag
     1681 tgagaaagct ttccattgaa ataagtcaaa taaaagttga tggcttcagt gctaaagtga
     1741 ggtgcccgct agccgtattc actcgaatcg gaattcaatg tattgcttct gccatttcca
     1801 taatatttca cgggaaaaac aaaatctgaa aaactatact taagtgttgt attgagtcag
     1861 ttgagataca aatacatatt tatcagcata cgttacgtca aatacaggag gttgtcatgt
     1921 tggtactgaa aggcacgttg gctattgcag ccgtgatgac aatttattgt cttatcgttg
     1981 ttcttatgga acgcttttct gattgatttc atattggcga gagaacaaga aggtgagtag
     2041 aattgtcgcg gaatcctgaa actatcagcc tcaggtatga acaccaaaag gtaggtagag
     2101 aaatctatgg cccccgttac tttattcgca caatgaggta tttttttatt tttacaaaag
     2161 ttcaataaat attataataa aagatatgtt cttggtaaca taaacaaata tttaattaaa
     2221 ataatgcttg gcattgacgt ttatcttata aagtaagaag atcatggaca tttcaaccaa
     2281 aaaagtagag ttctcgatga aattaaaatt ctttgtactt gcactttgtg tacctgcgat
     2341 ctttactaca catgctacca ctaactatcc acttttcata ccggacaaca tcagtacgga
     2401 tattagtcta ggatctctga gtggcaaaac aaaagaacgc gtttatcatc ccaaggaagg
     2461 ggggagaaaa attagtcaac tggactggaa atacagtaat gcgactattg ttagaggtgg
     2521 catcgattgg aagctaattc caaaagtgtc tttcggagtt tccggttgga ctactttagg
     2581 taaccagaaa gcaagcatgg ttgataaaga ctggaacaat tccaataccc ctcaggtatg
     2641 gaccgaccag agctggcatc ccaatacgca tctccgtgat gctaacgaat tcgagctgaa
     2701 tcttaaaggt tggttattaa ataatttgga ttatcgactt ggactaatag caggttacca
     2761 agagagtcgt tacagtttta atgcaatggg agggagttat atttatagtg aaaacggggg
     2821 gagcaggaat aaaaaagggg cacatcctag tggtgaaaga acaataggtt acaaacagct
     2881 cttcaaaata ccttatattg gattaactgc taattaccgc catgagaatt ttgagtttgg
     2941 agcagaactg aaatatagtg gttgggttct ttcatctgat acagataaac actatcagac
     3001 tgagacaatt tttaaagatg aaataaaaaa ccaaaattac tgctctgttg ctgcgaatat
     3061 tggatactat gtcaccccca gtgcaaaatt ttatatagaa ggctccagaa attacatttc
     3121 taataaaaaa ggtgatacat ctctttatga gcaaagtacc aatatatctg gcaccattaa
     3181 aaatagtgca agtattgaat atattggttt tctcacttcc gcaggtataa agtatatttt
     3241 ttgaattggt tcaccgagtt gattacgttt tcatggctaa aaattgccgc ccattgtgag
     3301 tcatattcgc cctgactttc cagaaccata cgaactgccc gttgacggac ttcgggggaa
     3361 aaacgtgtat ttttagtcat tctgtttacc tctttctcag ggcgtttagt ctccaggatt
     3421 tccggggcgg ttcaaaaaac tcttcgttga ttactgtgga cttactgttg gcattaccga
     3481 ccctgagacc ggagaaataa gaaccgctta gatcatcgtg gctgttctcg gggtatcaag
     3541 ttacacatgg gcagaggcta cccaaggtct actattatcg cctgaaagcg ctgatggaac
     3601 agtgctatca gggacatgct gatgaaaata cctaaataat cagccagcac tctatctttc
     3661 caaatccaca gcatagcaaa gagagcaaaa gagcctgtaa attcagaaat taaggaaacg
     3721 cctcttggtg tacataatct atcttatgtt aaatgaaatc taatttcttg attatatgct
     3781 ggatcgttcg tgggtggctt tgcccctata tttccagaca cctgttatca ttggcccgct
     3841 gccgcagata ttcccgtggc gagcgataat ccagcgcact atgcggatgc cattcgttat
     3901 aatgctcgaa tgcctctgca aggttctttg ctgccgttaa cccgtcaggt ttgggcatga
     3961 tactgatgta gtcacacttt attgttttca cgaagctctt tgctattctg ttactctccg
     4021 gactccgcac cgccgtgttc ttcggttcaa caaagtccca acatccgggt gaactggcgt
     4081 gtttcattag ctcggtagca tgaaccatta tccgtcagtc acttcactgg agacgccgga
     4141 agctcgttgc cgaagcggtg ttccaccgct cccagcatgt cgccaacggg agagcatcta
     4201 cggtctgatt gaactgttta ttcaaattgt ccaccgtctg tcagtacgct ctgaacgtcg
     4261 gctggttaaa acactgctcg ctgattttca gagggttcac ggtaaaacag cacttttatt
     4321 ccggattgcg gaagcggctc tgaataatcc ggatgggctg gttaaagagg ttgtgtacca
     4381 tctgcatatt gttgaaccag atcgcagcgg taagagaagt tcatcatact tggcgcagtt
     4441 acgcgatgtt tctgctagag gtgatgccgt aaagaacggc agaacattac cggaacaaga
     4501 tagcggtcta cctgcactgg tatcagaccc ggggcttccc cggatgatct ccacggtact
     4561 ttaggtactg tgtctatatt atgtaggtag gatcaattaa ctttgtagtt gaaattcttt
     4621 cattgaaata atgaataaaa aagacaaacc gcattaaaca atgccagaat ataattgcat
     4681 ttttgttccg tttttttaaa tcatagtata caaagaagct tgcatactat atgaaaatat
     4741 aacacccttt actatgatgt ttataaaaca atatatggag tcatgtaggt atgaatttag
     4801 atggtgttag accatactgt agaatagtca ataaaaagaa tgaaagcata tcagatattg
     4861 catttgcaca tataataaaa agggtaaaaa attcatcatg tactcaccca aaagcagcat
     4921 tggttttttt aggagagaaa ggtttttgtg atagcaatga tgttctatct attatgggac
     4981 aacaaatacc aagagtattt aagaacaaga tgttatatga ttatgttttt aaaaatgaaa
     5041 aaagtaaaaa tgattttcta aaaatggctg aatcatggct accacagagt gaaccaatag
     5101 taataaataa tgatgatgac gcattgaatg ctgctgctta tttttctgta aaaaaagcga
     5161 aaataaaaac agtaaacgat actgatttta aagagtataa taaggtttat attcttgggc
     5221 acggtagtcc tggttctcat caattaggcc ttggttcgga acttattgat gtacaaacaa
     5281 tcatttcaag aatgaaagac tgtggtattc taaatgtgaa agatatccgt tttacttcat
     5341 gcggctccgc tgataaagtg gctcctaaaa attttaacaa tgcccctgct gaaagtcttt
     5401 cttgtatcct taactctctg ccttttttta aggaaaaaga atctttgcta gagcagataa
     5461 aaaaacacct tgaaaacgat gagtcattga gtgatggtct aaaaatatcc ggctatcatg
     5521 gatatggagt tcactatggt caagagcttt ttccctactc acattatcgt tcaacttcaa
     5581 ttcctgctga tccggagcat acagtaaaaa gaagctctca gaaaaagact tttattatta
     5641 ataaagaact ggattagtat aaaattttta acctatagtt aattgtttgc atttaagatg
     5701 aagttatttt atctttagta gaaaagtgct tccctatgag ggaaaatcac aaatcatcat
     5761 aatacagaga caaaacgata cgaaaaaata gataaaaaac atcattcttt taccacatta
     5821 ttttccgtga atatgaaaaa taatgttatt actttaatat aagactattt tttgtttttc
     5881 catcactctg ttcaaatttt tccgcatgac ttgtgttttt tgtaatacag ctcgtttttt
     5941 acagctgacc aaaatcatca attaattatg ctaaggaaat aaattatgaa aaccaaaaac
     6001 tttcttcttt tttgtattgc tacaaatatg atttttatcc cctcagcaaa tgctctgaag
     6061 gcagaaggtt ttctcactca acaaacttca ccagacagtt tgtcaatact tccgccgcct
     6121 ccggcagaga attcagtagt atttcaggct gacaaagctc attatgaatt cggccgctcg
     6181 ctccgggatg ctaatcgtgt acgtctcgct agcgaagatg catactacga gaattttggt
     6241 cttgcatttt cagatgctta tggcatggat atttcaaggg aaaatacccc aatcttatat
     6301 cagttgttaa cacaagtact acaggatagc catgattacg ccgtgcgtaa cgccaaagaa
     6361 tattataaaa gagttcgtcc attcgttatt tataaagacg caacctgtac acctgataaa
     6421 gatgagaaaa tggctatcac tggctcttat ccctctggtc atgcatcctt tggttgggca
     6481 gtagcactga tacttgcgga gattaatcct caacgtaaag cggaaatact tcgacgtgga
     6541 tatgagtttg gagaaagtcg ggtcatctgc ggtgcgcatt ggcaaagcga tgtagaggct
     6601 gggcgtttaa tgggagcatc ggttgttgca gtacttcata atacacctga atttaccaaa
     6661 agccttagcg aagccaaaaa agagtttgaa gaattaaata ctcctaccaa tgaactgacc
     6721 ccataaagct ggacagcctg tatcaggcta tggagggccc atagacaaat ctaccctata
     6781 tgagcatagg aggagtctat gggcacacca cgttttaccc ctgaatttaa gggattactg
     6841 gaaaggctgg gacatatccc tccggcagaa gcagaaaaag cttattatgc tgccatcgga
     6901 aacgatgatc tggcaacctg agttcacaga taaaacattc tctaggaaac tcggggcggt
     6961 tccgttcacc acatgcaatg tggtgttgca ggggaacggt ctgcaccatc ccctatgtcg
     7021 atcaatataa cagaaatgac aacttcagat tcagggcaca acctaaatat attttaggtc
     7081 acctctcaaa tcgtttgcct gataccgctc cattttttaa taaaaaaatc aatcattttt
     7141 gaatctttgc ttttcattgc attgtcaagc atagtgtccc cactatttgc tttttgaaat
     7201 tttttgttta catcaaccaa tccattattg ataaaatatt ctagcacttt atagttggca
     7261 ccatctttac taagtaagta ttcaacatca tacagattac aatatctatt gtttttcatt
     7321 ttctccatct ctgcgacatc ttgttttgta aaattaaatt tagatagtaa ataaagtgct
     7381 atatcagctt ttttattgcc taaagaataa gcaagggcct gatgagccat ttttttatca
     7441 gtcactttat tgttaatctc ttcataatta gatgctttaa tcgcatcctc caaagatatg
     7501 tctctgagtt ttattttttt gaagttatct gtacttagca ttcttggtat atgatactca
     7561 agctgaaaca caaagtttat aattctatcc agactaacag gatcaatatt tttgttataa
     7621 caaaactctc tgaatccttg atctttactc tttcttataa aatcaataag atgcaaccca
     7681 attccaagcc tcatatcttt agtgttaaat attggaacat cagttttccc ctgattacca
     7741 tgcacatacc tgctaacttc atccacaacc tctttaaagt tatctaagaa aaatgattgg
     7801 tgctcatggt aaaaaacagg tggaatagca ttatcaaagt gatcggttaa tgtcatatat
     7861 ccatatggag agtcatgatc aatgatatac gcattggcgc caaaatctac agtatgatgt
     7921 gttttattta gtgggagttt ttcttgatga ttggaaattt cgaccccaaa aaaaacaaaa
     7981 tcgtggttag agatttcttt aatatcctca gagtatgtat tcttatgtgt ttgtatattt
     8041 tcagactgaa ttttatca
//
LOCUS       DAJTRH010000148         7949 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTRH010000148 DAJTRH010000000
VERSION     DAJTRH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(1..176)
                     /locus_tag="OO339_003692"
                     /pseudo
     CDS             complement(1..176)
                     /locus_tag="OO339_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            174..498
                     /locus_tag="OO339_003693"
                     /pseudo
     CDS             174..498
                     /locus_tag="OO339_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(528..3440)
                     /locus_tag="OO339_003694"
     CDS             complement(528..3440)
                     /locus_tag="OO339_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928159.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3443..3559
                     /locus_tag="OO339_003695"
     CDS             3443..3559
                     /locus_tag="OO339_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5928160.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3923..4765
                     /locus_tag="OO339_003696"
     CDS             3923..4765
                     /locus_tag="OO339_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5928161.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4768..5856
                     /gene="capU"
                     /locus_tag="OO339_003697"
     CDS             4768..5856
                     /gene="capU"
                     /locus_tag="OO339_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5928162.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGVDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5861..6811
                     /gene="virK"
                     /locus_tag="OO339_003698"
     CDS             5861..6811
                     /gene="virK"
                     /locus_tag="OO339_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5928163.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6876..7820
                     /gene="lpxM"
                     /locus_tag="OO339_003699"
                     /gene_synonym="msbB"
     CDS             6876..7820
                     /gene="lpxM"
                     /locus_tag="OO339_003699"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5928164.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2139 a         1776 c         1886 g         2148 t
ORIGIN      
        1 ttttttacca ccgggagttt gggcataacg gtgctttcag taagtacgac aggtactgtg
       61 gaggcacttc cgtaaagaag ggcatcaagc tcctgctccg acatacctgc aggcaaaggc
      121 catgaaagcc ctgatttccg gaagcgagcg aacagtccac aaactgttga acgtggtgtc
      181 agcggcggac agggataagc cctcgtcagc agttccgtca gcactgcgac agcgttcttt
      241 acccggtcaa aacagcgttg cggtgcacca cgcctgacgg atgaactgcg tgctcagggt
      301 taccacttta acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct gagggcaaag
      361 gcctcccgga agttcagccc ggtcagttac cgcgcacatg gcctgcccgt gtcagaaaat
      421 ctgctggagc aggattttta cgccagtggt ccgaaccaga agtgggcggg agacatcacg
      481 tacttacgta cagatgaagt tcggttacat cctgtcagca cggaacctca cgctttttga
      541 gcggcacgac ggatcccccc cacccagtat ccgacagact tttcactgcg ttcgtaaaaa
      601 cccttcttcc ctcccagcat atccactaat tccggtgaca tatcctgcag ataagtctgc
      661 aaatcatgcc ccagatcttc cggtgactgc aactcataaa ccgcgcgcca cacatcaccc
      721 ttccttattt taagccccgg gttaagaata tactgtccgt ggttcagcct cagaaaaagc
      781 ttttttccat aaggattttt gctgttaact tcatgttgag agagctggct gctgatataa
      841 ctccttcgtt tgcggcgttc cggcagcaac gcttcaggaa gatgaaccac acactctgcc
      901 agtcgtgccg cactgaaggc ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac
      961 agcgcctgca tcaggatcac cagaaacatt gccatctgcc ggttgtccag tttgatgagg
     1021 cgtccttcca cctgcagtga cagactttca ggtttcagca gggtatacaa ctgcgcactt
     1081 ttctgttgcg cataacgggg cgtactgacc gccgctgaca gaacttgatg ataagccgcc
     1141 agcccgtcat taccggtcag caagggatct gcaccacgct ccagcagtaa ctgaaccagt
     1201 gcaatattac ctgcccgggc tgctgacatc agcggagtaa gattaaacgg cgaacggtga
     1261 tcaacaccat aacgttcaat atcccgcaac acggcaaccg ggttattcat tgagtatggc
     1321 cggaaatact tcagctccag taactgcaga gtcttcgttc tgggctgcct tgtgggctta
     1381 aaatcttccc ggtacagtgc attatacagc gggaacaggg aataaatcag actgtactcc
     1441 agcgcctgca gttgtatggt tttatcggct gtacctgcga ggatatgctg tttccactgt
     1501 cgtgcatcct ctgcggtgat aatctgccat ggcatctcag atgtctgcag gatccggcta
     1561 cggatggtgt ccgcctgctc ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc
     1621 cactcatcac ggctggaaat ctcttcctgc agattcagag gctcttcctg atgcgtgagc
     1681 gcaagcagac tccacagagg atgctccacc tgctgttcaa caagatacac attgtgttta
     1741 gcacgggtga gcgcaacata cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga
     1801 tcctgcctgt cccgcggacg ggaataacgg gcctcgccct caagatcagc cggtgtaaga
     1861 ccctcgcaaa tatcatcaaa tgcctgccgg gcagcgctta ctatattgta aaggatcacc
     1921 gtttcgtatt ccagcccctt aacctcctga accgaaaaga ccagcggcgt actgaaccag
     1981 catcgcgcct gtgctttctg ttccggatgc atcacaatga ccgctacacg attcgaaagt
     2041 gaggttttgg cattcagttc ctgctttgtc tcttccctat cgtccagcaa ccggatggtc
     2101 ccttctgcct gaccgcagct tctgacaaaa tggtgacttt cccgatcaat agcactaaag
     2161 cggacctgtt tgagtctcag caaccggtta gccagtgcag tgacgtggtg cccgttacgg
     2221 taattcgcct gcaggatatt aacggttgtg tcgtttccct gttgctgccg gaaaaagaga
     2281 cttttgagcg aactccatga gaaaaagctc ggatgaacta tctgattggc atcaccacag
     2341 agtaaaaact gaccgggatg acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc
     2401 atatcctgaa cttcatcaac aaaaatgaca tcataacggg gagttgccaa cgaaagatac
     2461 tgatgactga gcagattact gtcatactga tgcgactgtt tcagccatgc gatataacgt
     2521 tcaaacagga catagaccgt cgggcggtcc tccatgccat agagcgactg ccgtatcccc
     2581 agtgacagat acgcctcccg ggacagcggc ccattcccgg aggcaaccgc accgatcacg
     2641 cccctgaact cctcatacag ggtatgggca gcccccagtg cggcgatagc cctgttgcgg
     2701 ggcagccagt cactgaacgc actgaaagtc acctccctgc cttccggaat gcgcagagtt
     2761 tccagaaatt ccgtcagcga aagaaagtca atattctgta cctcactccc ttcccctgaa
     2821 gcgtcataga gagtacgcgc tttttccacc agaaatgagg aaaggctgag atacagtata
     2881 tcaccagcag cctgcttcat tttttccagt accagcgccg tcttaccact tcccgccgag
     2941 ccaacaatca ccagcggtag cgggtgttcg tacagtgcct gctgatcatc atcaaaggac
     3001 agcattttat ccaggcggtg gaaacgccca tgagacggat tgatatacgt cagcgcttca
     3061 gtcgcgatat caacaggatc cggcactggt tgttgttgta agcgcccttc atcaatcacc
     3121 acgtttctgc gaagaaaacg tgaagtgtta taggcatgat tgcgaatata ttccagaaca
     3181 aggcagatag tttcaccgtg gtagcgatac agcgaaaaca gtaatcggtc ccggatactg
     3241 agccgggcgc ggtaaagatt ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg
     3301 gtggcaatag cctgtgcaaa ttttttataa cccgggatcg tgtcaggatt cagagtatga
     3361 taaagacaga ttttcatggc tgccggtttc cgtcgtgcaa aaaacatgct gattttacga
     3421 aggaatgcct tactgaacaa ccttgcccgg aatacccccc gaaatcagat ggaacaatca
     3481 gaaaaatgct ctgcagttat ggaataaaga aggtggggtg gtacgccggg agggcgatcc
     3541 gctggaaatg gcgcagtagc tgcaggcctg tcatgatgca ggaatagaag ttcgtgtaca
     3601 ggttaatgaa agcgtcaccc cgtaaataaa ctctgcgctg aaattccttt ttttcattgg
     3661 agtacctgtg ttgttctgag gtgaacatat cacctctgtt caggtagcca aattcagtgt
     3721 gccacctcaa ggttcttagt gagtcgtttt taaaagatat gataatatat cattgtttat
     3781 gaattgttga tgtttaattt agtaaattat atcaaatatg ccatgggttt gctatgttat
     3841 tcatccagta tctggaaata aaatgcacca gctttattgg gttttccttt tgtctctgct
     3901 tgctaggtgc cagaaacttt aaatgttaaa cgaagggggc attttgttca aagccaatca
     3961 tgttccggta ttaatgtatc atcatgtcag tcattgcccc ggactggtta cactttctcc
     4021 cgtgacattc cggaagcaaa taaaatggtt ggctgaaaac aactggaaaa cactgtcatc
     4081 agatgaactg gagttttttt atcggggcgg aaaactcccc cggaaaagcg ttatgttgac
     4141 atttgatgat ggataccttg acaactggtt tcaggtatat cctttattaa aagagtttaa
     4201 tctcaaagca catatttttt taattaccgg ttttattggg aatggtccgg ttcgtcactc
     4261 tcctggaaag gaatattctc atcgcgattg cgaacatcaa atagcgacag ggaatgctga
     4321 taatgttatg cttcgctggt ctgaagtaaa tgaaatgttg caatctggtc tggtcgaatt
     4381 tcatgttcat acgcatactc atacacgctg ggataaaaag ttcagttcac gagaagaaca
     4441 gtgtaaacat cttcggcagg acttgttgag cgggagggaa tatttgaaag aaatgacagg
     4501 aaaatgcagt aaacatttgt gttggccgga aggttattat aacaaggatt atattcaggt
     4561 cgctgaagaa cttggattct attatttata tacaacagaa agaagaatga atgctcctgc
     4621 taaaggaact acacggatag gaagaataag caccaaagaa cgggaaagtt gcgcatggct
     4681 gaaaagacga cttttttatt atacaacccc gtttttttca tcgttgctgg cctttcataa
     4741 ggggccaaga ttaccggatg attaataatg aatatactat ttacggaatc atcaccgaat
     4801 ataggtggtc aggaattaca ggctgttgct caaatgaagg ccctgaagaa aatggggcat
     4861 tcagttctgc ttgtctgcag ggaaaacagc aaaattgctt ttgaagccag taaattgggg
     4921 gttgatatca cattcgcgtt atttcgaaac agtcttcaca tccctactgc atggagatta
     4981 ctcggaatag ttcatggttt tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc
     5041 aatattgttg gtttagtacg gttattcact aggaaacatc cattcagaat tatcaggcaa
     5101 aagacatatt tgacacgaaa aacaaaagtt ttttcaataa atcatttttg cgatgaggtg
     5161 attgttcccg gaacaagtat gaagacacat ctggagcagg aaggatgtcg aacccgggtt
     5221 actgttgtgc ctccaggctt tgatttccag aaattatacg ttgattcccg aaacagtttg
     5281 cctccaaatg ttctttcttg gctggcgtcc cgaaggggat gccccgttat tgctcaggta
     5341 ggaatgttgc gcccggaaaa agggcacgaa tttatgttga atttactgtt ccatttaaaa
     5401 atgaatggac gacagttctg ttggttgatt gtggggtctg gttcgcctga actgcgggag
     5461 catttacagt atcagattga cagtatgggc atgcatgatg atgtttttat tgctgacaat
     5521 gtttttcctg ccgcccccgt atatcgggtt gccagtctgg tggttctgcc ttcagaaaac
     5581 gaatcgtttg gtatggtact ggcagaagca tcggcatttt ctgtgcctgt actggccagt
     5641 cagattggtg gaatccctga tgttattcag aataaccaga ccgggacatt gttaccagca
     5701 ggtaataagc acgcatggat gtgcgccctg aatgattttt ttaatgaccc tgggcgtttt
     5761 tatcagatgg ctcgccaggc aaaacaggat atagaagagc ggtttgatat taataaaact
     5821 gcgttaaaaa tactcacatt agcgaagcac aagtaacaat atgttttctg taagtaactt
     5881 atcatttatc ggtttcctta aaaggattgt tttttcctca gattcactcc cggggaagtg
     5941 ggaacacaga aaattccggt tcatgtacat tttgcgatgt gctataaatc cggttgccag
     6001 tattcgatat tattacgaac tgcgttcctt gcagtgcatt gaggatattc tggcaataca
     6061 acccacgttg ccagcaagaa ttcatcgacc ttatttgcat aaaggggggc gagcctggtc
     6121 ccgggggcaa tatattcttg agcattatcg ctttgtgcaa aatttgccag aaaaatattc
     6181 tgaatttttg tttccacaaa aatcggtatc attagtccaa ttcatcggga aagatggaga
     6241 ggattttgat atccagtgtt ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc
     6301 attgtttttc aataaaatag tgattgcccg gttaacattt tcagtcattc tgactcaaaa
     6361 tggacacacc gcttttattg gcggattaca gggagccccc aaaaataccg gacctgatat
     6421 tattcggtgc gcaacccggg cctgttatgg gcttttccca aaacggatta tttttgaagc
     6481 attttgtgcg ttgatgaaag cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag
     6541 tcatgttttc cggcaattga gatactggta tcagaaacgc aaaacctttg ttgctgtcta
     6601 cagtgatttc tgggagtctg tagcagggaa aacctgtggt gactggtaca aattaccaac
     6661 acaggtggtt cgaaagccgc taagcaatat agctagtaaa aaacgctctg agtatcgaaa
     6721 acgatacgct ttgctggatt atatccatga aactgctatc cgctctttgg atgcatatcc
     6781 tgtaaactca gaacatcagg acttaaatta aggtttgatg tattctctga ataaaatatt
     6841 aatgatgatt ttggtagggg cattcgcact aaataatgaa aaaatacaaa tccgagttta
     6901 ttcctgaatt taagaaaaat tatctttccc ctgtttactg gtttacatgg ttcgttttgg
     6961 gaatgattgc aggtatttca atgtttcccc cttcattcag agatcctgtc ttggccaaaa
     7021 tagggcgttg ggtgggcaga ttgagcagaa aagctcgtcg cagggcgacg attaatttat
     7081 cgctttgttt cccggaaaag agtgatacag aacgggaaat aattgtcgac aatatgtttg
     7141 ccacagcatt gcaatccata gtgatgatgg cagaactggc aattcgtggt ccggaaaagt
     7201 tccagaaacg agtgttctgg aaaggcctcg aaattcttga ggaaattcgg cacaataaca
     7261 gaaatgtgat ttttctggtt ccccatggct ggagcgtgga tattcctgca atgttgctgg
     7321 cagctcaggg ggaaaaaatg gccgccatgt ttcatcagca acgaaatcca gtgattgatt
     7381 atgtctggaa ttctgtacgg cgtaaattcg gggggcgctt acattcccgg gaggatggga
     7441 taaaaccatt tattcagtca gtacgccagg gatactgggg gtattacctt ccagatcagg
     7501 atcatggtcc tgaatacagt gaatttgctg atttttttgc gacctataaa gcgacattac
     7561 caattattgg acgtctgatg aacatcagtc aggctatgat tataccgctt ttcccggttt
     7621 atgatgaaaa aaaacatttc ctgactattg aagttcggcc accaatggat gcatgcattg
     7681 ccagcgcgga caataaaatg attgcccgac aaatgaacaa aacagtggaa attttggtgg
     7741 ggtcacatcc ggaacagtat atctgggttt taaaattgtt aaaaacgcgc aaatcaaacg
     7801 aagcggaccc gtacccttga aattataaag tacaggtatt tccactagtt gtttcttaca
     7861 ggttaccaat cgaaacacat cccccttccg tgtgttgccc gtttggcacg gaggactgtg
     7921 tagatgcagt tcgttaaatt taatttatg
//
LOCUS       DAJTRH010000149         7839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTRH010000149 DAJTRH010000000
VERSION     DAJTRH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            7..657
                     /locus_tag="OO339_003700"
     CDS             7..657
                     /locus_tag="OO339_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928165.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="OO339_003701"
     CDS             629..1063
                     /locus_tag="OO339_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5928166.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2885)
                     /locus_tag="OO339_003702"
     CDS             complement(1836..2885)
                     /locus_tag="OO339_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5928167.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3336..4526
                     /locus_tag="OO339_003703"
     CDS             3336..4526
                     /locus_tag="OO339_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5928168.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO339_003704"
     CDS             complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO339_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5928169.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6373..7302)
                     /locus_tag="OO339_003705"
     CDS             complement(6373..7302)
                     /locus_tag="OO339_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5928170.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7299..7661)
                     /locus_tag="OO339_003706"
     CDS             complement(7299..7661)
                     /locus_tag="OO339_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5928171.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         2438 a         1485 c         1585 g         2331 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc caggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgtgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa
     2761 ggatacaatt tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga
     2821 aattttaaag catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta
     2881 tacatttaaa gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa
     2941 tgcctgaaag tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag
     3001 tcgcactata taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc
     3061 tgcggccgcg ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca
     3121 agataatcgt ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc
     3181 tgccactctc gcaccatggt catagccttt acgatctgca tactcaccag accagagccc
     3241 gctccctttt aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta
     3301 ctgattgcgc taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga
     3361 cttttttccg taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt
     3421 ctgctgctgc tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac
     3481 ccggtcctgc tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac
     3541 gacttcataa tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac
     3601 tgtcgtggtg tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc
     3661 ttcgtgccag cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt
     3721 caaaaaaaaa taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt
     3781 ctccccggac tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc
     3841 atatcaaatc acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc
     3901 tggatagtga caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa
     3961 aatacctggg gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt
     4021 atctttataa acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct
     4081 taccaacaac ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca
     4141 atacagctga gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag
     4201 agcaaaactt tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa
     4261 gtcggagggg agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac
     4321 tctggctact tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata
     4381 gtctcaaaaa acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta
     4441 accctgaact tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt
     4501 accgaagtat ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg
     4561 gaggaatgag ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat
     4621 ttggcctgag tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg
     4681 ttaaacttca attctcagtg ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt
     4741 tttaattgac taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg
     4801 tatttaaagg ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat
     4861 tattttgagc ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa
     4921 tattaaatgg aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt
     4981 cagctatcga atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct
     5041 ctatttgttc tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa
     5101 ccatcctaaa atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc
     5161 tcatggagag catatttcct gagtcgtttg aaccatatat atatatatat tttccgttaa
     5221 gggaaatgcc ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct
     5281 gtcttgcgag agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa
     5341 taaataaaaa aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa
     5401 acaaaattac tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa
     5461 caacaacgat aaatataacc ggaagtatat aaatgaaaat gattgatgaa atcaaataaa
     5521 ttgatcttgt ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta
     5581 accacttgta taggtttaaa ccagtgggag gattgttata gaagttatat atattatcta
     5641 tggctgattt aacaagccat ttaatatcgc ttgcattttg tatataattt gagatgtact
     5701 cgttcgattc aagcgaaaaa taatgcttga tagctttggt taatagcagg taaattaagg
     5761 tagataaagc tatgagggat agagataaac atgaactata aaaagattca cgaatatttt
     5821 tctcatttcg tattatcgaa tttatctgca acccaatgac gaatgcaata atatatgtta
     5881 cgaatgtttg gtagattgcc attgaaagaa tggacagtac tataccagaa aatatcacaa
     5941 tcctattttt ttgcgagtga aaaataattg ctgatatcgc tgccagtaga aaagcaattc
     6001 ccacagtatc agcttggtta agaaactcta attgatagga gatctgaggg aaggtaataa
     6061 atactaacat acctatcaat aattcataag attctagctt tagcgatctg cagattataa
     6121 atgctgaaat gatgataaaa gataaggcta ttaatggtgt tatataaagt gaaaaaggct
     6181 cagggagaaa gtaatgtcgc aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat
     6241 aaaaattatt tgtaaactcg ccatcaatat taagtgttcc atcgtacaac cacccccaat
     6301 aacaataaat tgatattgca aaacatgata tgaaggcaag gagatttgca tttttaaata
     6361 aattaatttt aatcatgggt tacctcgatg agattttttt atgatgtatt tgggtctatt
     6421 ttttacttca atatagattc tacctatata ttctccgaga acaccaatcc cgatcagttg
     6481 cactccaccc aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc
     6541 aaagacaagg gtgtctataa tcatccatgc accatataaa aatgaaatgc ttgcaacaaa
     6601 caagcctata taagtccata cgcgaagagg aaaggttgaa aaacttgtga taccttccag
     6661 tgccagattc cataatttcc agccattaaa ttttgagatg ccagcaacac gctctgcgcg
     6721 tacatattca acgacatccg tcggaccacc cacccagctc agtatgcctt tcatgaaaag
     6781 attgcgctca ggcaacagtt taatgttctc cacaacctca cgagacatga gtcgaaaatc
     6841 tccgacattt tcctcgatct ttggggtgct tattttgttg tgtaatttat agaaccactc
     6901 agctgtctta cgtttcagtc gtccatcagt tgagcggtct gaacgtttag caagcaccat
     6961 gtcagcacct gcctgccact tttcaataag atgagggata acctcaattg ggtcttgcag
     7021 gtcgacatca ataggaatta cagcatcgcc gcttgcatgg tctaaccctg caaataaggc
     7081 tggttcttta ccaaagttgc gtgtaaatga cagcggaaca actagcgggt ctgaaacagc
     7141 cagcgcgtta ataattgact ctgtggcatc tttacttccg tcatttatga atacaatttc
     7201 tacttcatat ggcttcaact cttggaattc acgtaccgtt ttatagaaaa caggtatcgc
     7261 ttcttcttca ttgaagacag gaacgacaag agatattttc atttcgcatc cctaaagaca
     7321 atgaactttg aatagacgaa accgcacacc agactgatgg cggagaaggt gataagagtg
     7381 actattggag gaagtgagca tttatcagct gcccaaccaa caatcacact caatattccc
     7441 ataaatccca cgtataacat gtagcgcatc gctgtagtca atgctttgaa tgtgaatctt
     7501 gcattcgcga agaagctaaa gctcacagcc actacgaaac ctgtgaagtt tgccagagcc
     7561 tgactggtat gtgcggcata gatacataca ccaaaaacca cccagtgtat aagtgtgttc
     7621 agcacaccta tagatgtgta ctttacaaat aactttaaca tttatttaat caatgagctc
     7681 tgaaaggcat gaagtctatc atccaagtct caattgatcg atacttgcta tgtctgatga
     7741 gacaaaactg agacacataa ggcctcacaa tggcttgcaa ggctttacat gttttgatgt
     7801 ggtgggacgt gtgagcgcag tgttgatggg ataatcctt
//
LOCUS       DAJTRH010000150         7832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTRH010000150 DAJTRH010000000
VERSION     DAJTRH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..1332
                     /gene="yjeM"
                     /locus_tag="OO339_003707"
                     /pseudo
     CDS             1..1332
                     /gene="yjeM"
                     /locus_tag="OO339_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO339_003708"
     CDS             complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO339_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5928172.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYDLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO339_003709"
     CDS             complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO339_003709"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5928173.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO339_003710"
     CDS             complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO339_003710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5928174.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7028..7573
                     /gene="orn"
                     /locus_tag="OO339_003711"
     CDS             7028..7573
                     /gene="orn"
                     /locus_tag="OO339_003711"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5928175.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1772 a         2029 c         2123 g         1908 t
ORIGIN      
        1 cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg
       61 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
      121 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
      181 ctatggtagt gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt
      241 tggtctgctg gcagtagcat ggatgattct ggtcacagtc gttgcttcaa aggggattaa
      301 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
      361 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
      421 tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt
      481 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
      541 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
      601 ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa
      661 tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgatgct
      721 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg
      781 tattaccgga ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa
      841 gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg
      901 atggggatgc cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg
      961 ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg
     1021 aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca
     1081 cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg
     1141 acggtggttg tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg
     1201 gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg
     1261 ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta
     1321 gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag
     1381 gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc
     1441 tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg
     1501 acgacctttg cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag
     1561 acgcatctgg aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat
     1621 cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg
     1681 cagctcgaaa atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt
     1741 gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact
     1801 gttggcatcg gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga
     1861 ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc
     1921 ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg
     1981 gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat
     2041 aaagttggcg aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag
     2101 ccactgcaat ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag
     2161 catcagcaga tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag
     2221 gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat
     2281 gataaatacc agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac
     2341 gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga
     2401 atggatttct gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg
     2461 caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc
     2521 aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc
     2581 taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag
     2641 catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt
     2701 ttcaagcctc gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat
     2761 ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc
     2821 gctgccttct ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac
     2881 cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg
     2941 gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta
     3001 gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
     3061 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac
     3121 accatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagt cgtaacccag
     3181 cgtcatccac agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa
     3241 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
     3301 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag
     3361 cggcaaaatt gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa
     3421 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt
     3481 catcgggcgc acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt
     3541 cagcgcatcc tccagttgcc tgttggagac tttcagcttg gtcagttcca atagtagcgt
     3601 gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag
     3661 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag
     3721 cggctgttta ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc
     3781 ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc
     3841 ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc
     3901 ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat
     3961 ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac
     4021 gatatctttc ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg
     4081 ctccgcctca agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc
     4141 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt
     4201 attggcagac agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc
     4261 agactgcaac gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag
     4321 gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt
     4381 cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc
     4441 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg
     4501 cgacacgctg cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag
     4561 tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg
     4621 ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt
     4681 cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc
     4741 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
     4801 gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg
     4861 ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt
     4921 ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc
     4981 aaccagattc actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg
     5041 acccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt
     5101 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
     5161 gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa
     5221 aaggcaaatc acgcgttcgt tacgggcaaa cagatttggc acgttctgag cggtgagatg
     5281 gttaacggaa aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg
     5341 catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg
     5401 gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc
     5461 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
     5521 aggcatgacc agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
     5581 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
     5641 gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
     5701 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
     5761 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
     5821 cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
     5881 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
     5941 acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat
     6001 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc
     6061 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
     6121 acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc
     6181 ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt
     6241 ttgcagcccc agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa
     6301 aatgctgatg cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct
     6361 ggaaaccatc aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa
     6421 cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg
     6481 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
     6541 ggcggagaca atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa
     6601 atccggacgc gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
     6661 tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga
     6721 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
     6781 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
     6841 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
     6901 ctgctggcct ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat
     6961 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
     7021 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
     7081 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa
     7141 tattctggca gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat
     7201 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag
     7261 cacgatgggc gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc
     7321 tgcgggaaaa tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa
     7381 atacatgccg gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa
     7441 agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca
     7501 tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt
     7561 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
     7621 attttgacgc taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa
     7681 aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
     7741 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa tagctcagtt
     7801 ggtagagcac gaccttgcca aggtcggggt cg
//
LOCUS       DAJTRH010000151         7727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTRH010000151 DAJTRH010000000
VERSION     DAJTRH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..835)
                     /locus_tag="OO339_003712"
     CDS             complement(<1..835)
                     /locus_tag="OO339_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR5928176.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQ"
     gene            1017..2441
                     /gene="sbcB"
                     /locus_tag="OO339_003713"
     CDS             1017..2441
                     /gene="sbcB"
                     /locus_tag="OO339_003713"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5928177.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2484..2711)
                     /locus_tag="OO339_003714"
     CDS             complement(2484..2711)
                     /locus_tag="OO339_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5928178.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2725..3783)
                     /gene="yeeE"
                     /locus_tag="OO339_003715"
     CDS             complement(2725..3783)
                     /gene="yeeE"
                     /locus_tag="OO339_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5928179.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3962..5320)
                     /gene="plaP"
                     /locus_tag="OO339_003716"
     CDS             complement(3962..5320)
                     /gene="plaP"
                     /locus_tag="OO339_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5928180.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5310..5372)
                     /gene="yoeI"
                     /locus_tag="OO339_003717"
     CDS             complement(5310..5372)
                     /gene="yoeI"
                     /locus_tag="OO339_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5928181.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5533..6516)
                     /locus_tag="OO339_003718"
     CDS             complement(5533..6516)
                     /locus_tag="OO339_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5928182.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6562..7386)
                     /locus_tag="OO339_003719"
     CDS             complement(6562..7386)
                     /locus_tag="OO339_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5928183.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7469..7723)
                     /gene="yoeB"
                     /locus_tag="OO339_003720"
     CDS             complement(7469..7723)
                     /gene="yoeB"
                     /locus_tag="OO339_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5928184.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2069 a         2013 c         1812 g         1833 t
ORIGIN      
        1 tttgttcgaa gattggacgg cggataacat tacccatctt gctatgctct ttcggtacgg
       61 tccatacgtt gtccagcagg tcaaattctg tctttgttgc cagcctaagc tctgagagcc
      121 tcgcccccca cagcataagc atctgatgaa ggagcttatt tgacgtagac gcacggcttc
      181 tttcaatagc aagccaaatc ttagccagtt cgtgatacga cagtacccga tcccctacct
      241 cagcgcggga gccgaagtcc cttggctgga tgctcataat tgcgcaacta tctatcaact
      301 gacgccgcat gcaccaacta attgctgatc ttagttgact tagcacctgc cttgctcggc
      361 gtggattatc tttttcttct tcggtaagca ggtcaaccca ttgcttaacc gtgatagaag
      421 atgccggacg attagggaag gcgtcatgca tgcgcttcat aaccgttgat cgataaagtg
      481 cctgggtctt ttctctgaga gttgtagaga cgtagttgtc gaaccagtag tcgagacact
      541 gggcgaccgt catggagttc tccaccttct cttcaaaata ggtgcgtgga tccgttcctg
      601 agaaatagag ctttcgcaag tcagcagtga tctgtctggc atccttcaaa gacagggatg
      661 ggtatcgacc aagcccaagt cgattaggct tgccatgcca gcgatagcgg tactggaact
      721 ggatgacccc cttcggtgaa attcgtacgc tgaggccatc ggcatcagcc acttcttgtg
      781 ggcccgaata tggtttacca taaatagtac gcagttttgt gtcgcttata gccataaata
      841 atatttggta cgctcagaaa taatagtttg gcacacatct ggtacacaat atcacatgga
      901 caacagcgca cagtaaacaa ctatatttga cgttcgtaga catactcagg tgaaaaaagg
      961 gttgcttttc gagttataac agacagttaa ttaactacag tggacaatgc tagacaatga
     1021 cagacacaga taaacaacct accttcctct ttcacgatta cgaaaccttt ggcacgcacc
     1081 ccgcgttaga tcgccctgca cagttcgcag ccattcgcac cgatgacgaa ttcaatgtca
     1141 tcggcgaacc cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cggcctggag
     1201 cagtattaat taccggtatt accccgcagg aggcacgggc gaaaggagaa aacgaagccg
     1261 cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca
     1321 acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc
     1381 cttacgcctg gagctggcag catgataact cgcgctggga tttactggat gttatgcgtg
     1441 cctgttatgc cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga
     1501 gctttcgcct tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg
     1561 cgatggctga tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg cgtcagccac
     1621 gcctgtttga ttatctcttt acccatcgta ataaacacaa actgatggcg ttgattgatg
     1681 ttccgcagat gaaacccctg gctcacgttt ccggaatgtt tggggcatgg cgtggcaata
     1741 ccagctgggt ggcaccgctg gcgtggcatc ctgaaaatcg caatgccgta attatggcgg
     1801 atttggcagg agacatttcg ccattactgg agctggatat cgacacattg cgcgagcgtt
     1861 tatataccgc aaaagccgat cttggcgata acgccgccgt tccggttaag ctggtgcata
     1921 tcaataaatg tccggtactg gcccaggcga atacgctacg cccggaagat gccgaccgac
     1981 tgggaattaa tcgtcagcat tgcctcgata acctaaaaat tctgcgtgaa aatccgcaag
     2041 tgcgtgaaaa agtggtagcg atattcgcgg aagccgaacc gtttacacct tcagataatg
     2101 tggatgcaca gctttataat ggctttttca gtgacgccga tcgtgcagca atgaaaattg
     2161 tgctggaaac cgagccgcgt aatttaccgg cactggatat cacttttgtt gataaacgaa
     2221 ttgaaaagtt gttgttcaat tatcgggcac gcaacttccc ggggacgctg gattatgccg
     2281 agcagcaacg ctggctggag caccgccacc aggtcttcac gccagagttt ttgcagggtt
     2341 atgctgatga attgcagatg ctggtacaac aatatgccga tgacaaagag aaagtggcgc
     2401 tgttaaaagc actttggcag tacgcggaag agatcgtcta atttgaagcc gccgccgctg
     2461 gtacggcgga ggacaatgtt gagtcaggct ttttgaacgg tgatgctcca cgctgcatcg
     2521 ccaatctgct gataatcggt gatggcatgt ccttcttctg ccgcccactg tgggattgct
     2581 tcagttgcct gggtgcaatc aaactcaatt accaactcat cgccgctggc catttctgcc
     2641 agtgctgctt ttgcttctat gagtggaaac ggacagacct gcgtcaccac atccagcttt
     2701 ttaatcgcca tctttacacc ttacttaatt tgccgcagca gttgccagtc gcgccttacg
     2761 ctgcggtcga acatacacaa gccaggacgc agtccagact ccgagaatca taaatacaag
     2821 gccaatccag ccctgccagg tcatcattgc agtcataacc agtccattac cgatagagca
     2881 accacctgca atactggcac cgaagcccat cagtacacca cctaacccgc tacgtaatgt
     2941 tgtttgtgca tcagctgcgc gaacgcggaa ctcacggctc gctttggctg caataaatgc
     3001 ctccacgaag atccctaaca ccaggaaaac gccccagtta atgtatttca cgtcacccgc
     3061 gaccagaaat tgaagaatat tggccgttgg ggaagtgact cctaacccaa acatgcgtcc
     3121 ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagcagtgac
     3181 aaagggatgc cagcgttttt caaacagaat atgagcgatc ccggttcggc gcggcggtaa
     3241 ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac
     3301 cagcaaaacg gtaaccaacg gccacacaga caaattaaaa gtctcagcaa tagagttatg
     3361 ttcagtagtg tagtgctgca aggtttgatt taaaccactg gcgtgtggag aacgcatcac
     3421 cgcactcatc accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc
     3481 gcgataccag gtcccggtgg cacatccacc cgccagaaca attcccagcc cgaaaagata
     3541 cccacctata acagtaccaa gccacgggaa cgccccggct tcgtaagtca gtaggcccgc
     3601 ctgaattaac gcaaaaaccc ccacgctttg tgccgaaatt gcaatcagca aggcgtaaaa
     3661 catgcgatta tttttcgcga tatacatatc gcgaaaacca ccagtcaggc aaaaacgccc
     3721 acgctgcatg acaaatccca gcagagcacc acaaattagc ccgcttaata tcattgaaaa
     3781 cataaattaa ttaaccagat gaatgttaat ggggaaattt ttcatgactg gtggaatgca
     3841 gaccaataac caaattctct aaattagaac aaatggttat taatgaggat ttcgataaaa
     3901 aagaataaaa aaaccggagc caaatgttca ttcgactccg gtttattatt agaaaagatg
     3961 attatgctac gtcttcgtac tgcggaaccg gattacggaa gctcttggtg acgcaagcca
     4021 ggtaaatcag accgatagca gcccagatca gacccagaac cattgagctt tcttccaggt
     4081 taacccacag cgcaccaacg gtcagcgcac cacacatcgg caggaacaga tactggaagt
     4141 gatctttcag cgtcttgtta cgcttctcac ggatccagaa ctgcgagatg accgacaggt
     4201 taacaaaggt gaacgccacc agcgcaccaa agttaatcag cgccgttgcc attaccaggt
     4261 cgaagttgat tgccagcagg gcaatcgcgc caaccaggat gatgttcatc gccggagtac
     4321 gccatttcgg gtgtacataa ccgaagaagc ttttcgggaa cacgccgtca cgacccatta
     4381 cgtacatcag acgcgctacg cctgcgtgcg ccgccatacc ggacgccagt acggtaatgg
     4441 tggagaagat cagcgcgcca acctggaacg ctttacccgc aacgtacagc atgatttcag
     4501 gctgtgacgc atccggatct ttaaagcgag agatatccgg gaagtacagc tgcaggaagt
     4561 aagttgcaaa gatgaagatc atgccgccaa tcagcgcggt caggaaaatc gcacgcggga
     4621 tcacgcgctc tgcatctttg gtttcttccg acagattgct gatgccgtca aagccggtaa
     4681 aggagaagca caggattgtc gccccggtaa tcatcgggat aacatgcgca tcgccagacc
     4741 agaacggacg cgtgctcgcc agcgtaccag cgccttcacc ttcaaatacg ccataaacaa
     4801 ccatgcccag aatcaccgcg atcagcacta cctgcaacac gacgattaca gtgttgacgt
     4861 tcgctacgga tttcagacta cgcaggttaa aggcggtcat aaaggccacc agcgccacca
     4921 caaacatcca cgatggaatg gaaggcacca gagcttcaaa atagattttc gccagcagaa
     4981 tgttgatcat cggcgcgaac agatagtcga gcagagaaga ccaacccacc ataaagccga
     5041 cagtcgggct aatggatttc tgggcgtaag tgtatgcaga gccagcagaa ggatagcggc
     5101 gaaccagctt cccgtagctc agagccgtaa acaggatcgc aatcaatgcg aacgcatagg
     5161 ctgtcggcac atgaccatcc gtgaggcctg aaacgatacc aaatgtatca aacagcgtca
     5221 tcggctgcat ataggctaga cccatcatta caaccggaac taacgtaagc gttttacgca
     5281 attccacgcg agaggtgttt ggagtaacgt tatgcgacat ggttattctc ctttacggtg
     5341 ataaccgtcg cgtaagcaaa aaattgcccc atttttttgg attcctcagc gacaacaact
     5401 gtcgattttt agtaaatatc tatccggtac gaagcccggc ctcttggtat gaatgattgg
     5461 tttgaagcaa aaaataaccg acgctaatta aacgtcggtt tttaatcatt ttttgacagc
     5521 ggcgcattgt gcctaaatgg gggggaaatg acaagagaat gagaggcttg tcataataat
     5581 ttttctttga atggctgatt ttccgtcatc agattttgca taaacaccgg cgatcgcact
     5641 atttgctaaa atttcatctc gccaccagcc gctggcaagc cctgcggtct gttcattcca
     5701 gccaatccag acaggttcaa agagagtttg ggcgaccact ttcttctcga ttaacgcgcc
     5761 actatcgaga aaacgttgcg ccagataccg tggtaaatag ccgcagccaa ggccgctaat
     5821 ttgcagctcc agtttggttt taaaatcgaa aacggtaatg gcttcttgtt cgtcgagaag
     5881 ttgtgaggcc gtagaagcgc ctgcctgcgc agtatccccc accacaatcg cccgatatcg
     5941 tttaatgata cgacggttta acggttcttc ttcctgggct aacggatgat gaggcgcgac
     6001 cgcaaagact tgttcaagat cgcccaaccg cgagaaacca aactcactgg aagatggtgg
     6061 ctcatgcatc gcgccaacga taatatccgc tcgcccctga gtcagggcat cccaggaacc
     6121 accgagtacg ccattgataa acttcaggcg cgtcacgcta tgatgttgat aaaaagcttc
     6181 aatgagaggc gcaagcagag aaaaaggaaa cgtgtcgtcc acgccaatca ccagctcgtt
     6241 ttcccagcct tcatgcagtt tgatcgcctg cttttccagt tctcgcacgg tatgcagaac
     6301 ttcccgccct ttctctaata acatttttcc ggtgcgggtg aatttagcgc ggtgtccgct
     6361 acgatcaagc aattgaatat ttaggtcgct ttccagcttg tgaacggtgt aactcagagc
     6421 ggacggcgtc ttgtagagtt tggctgacgc cgccgcaaaa ctgccctctt tttctatggc
     6481 atcgaggatc atcaacacat ccagcagtgg tttcatacac gcccccttgc agtgcatgcg
     6541 gcgtcctgct ggcgtgatcg tttactccaa cggcattacc agcggatcgg gatactggta
     6601 ttcaaatccc agttcattac aaatccgact gccatcaata atcttacctt tgccgctgtc
     6661 cagactattt ctgaactgcg gaggttgcag ccccagtaaa cgggccatct gcggatagaa
     6721 aacattacgc gcagggtgag ctggcgcaca tatattatag atgtgtccgc ctttaggtgc
     6781 ctgtaacaac agagtgatag cgccaataac atcttctaaa tggactaaat taacaccatg
     6841 ttcaccatca ggggcggttt ttccggcaaa gaagcgtccc ggatgacgtc ccggtccgac
     6901 caggcccgca agacgcagaa tatcgaccga agtaccgggt aaattgtgca gccagtcttc
     6961 gagttcttct aatactcgtc cactgttggt taccggatta cgcggggtag tttctttaac
     7021 cgtgccttgc gcgtcgccat agacagatgt tgagctggta aaaataattc ggggaatacg
     7081 atgggccagc gcgctatcca ctaactcttg taccgcttgt aaatagaact catcgccggg
     7141 gccgctacga cgtgccggaa gcgtaatgac cagcgcatcg gcatccataa gggcatccag
     7201 atcgtcagaa tcgcaaacta actcaggctc catgcgaagc agatagctat caatgccact
     7261 cattcgggcc gcttcgacac catcttgtgt ggttttactc ccggtgactt gccagcctcg
     7321 cgctgaaagt gacatcgcca gcggcatacc taaccaccct aacccgacaa ttgcgacctt
     7381 tttcatccgt tatctcctga tacaccttag atctataagg ctacgctagc gtatcaaaac
     7441 tgacaattca ttctatgaat gaatctgttc aataatgata acgacatgct gcaatgagca
     7501 gtgaatcatc agtaaccgcg tataccagac ggtgctcctc tgtaatgcgt cgggaccaga
     7561 aacctgacaa attatgtttc aggggttctg gtttcccctt accttcaaat ggtgttctgc
     7621 gggtatcttt gataagttca ttgatctttt taacaattcg cttatctgtt tcctgccagt
     7681 acagataatc atcccatgat tcctcagacc agattagttt cactcaa
//
LOCUS       DAJTRH010000152         7456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTRH010000152 DAJTRH010000000
VERSION     DAJTRH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..695
                     /locus_tag="OO339_003721"
     CDS             <1..695
                     /locus_tag="OO339_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5928185.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1034..1804)
                     /locus_tag="OO339_003722"
     CDS             complement(1034..1804)
                     /locus_tag="OO339_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928186.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="OO339_003723"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="OO339_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="OO339_003724"
     CDS             complement(2295..2516)
                     /locus_tag="OO339_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928187.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="OO339_003725"
     CDS             complement(2517..3200)
                     /locus_tag="OO339_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5928188.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="OO339_003726"
     CDS             complement(3264..3575)
                     /locus_tag="OO339_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928189.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3572..4474)
                     /locus_tag="OO339_003727"
     CDS             complement(3572..4474)
                     /locus_tag="OO339_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5928190.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="OO339_003728"
     CDS             4914..5873
                     /locus_tag="OO339_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5928191.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="OO339_003729"
     CDS             5873..6268
                     /locus_tag="OO339_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5928192.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="OO339_003730"
     CDS             6451..6831
                     /locus_tag="OO339_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5928193.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6877..7456
                     /locus_tag="OO339_003731"
                     /pseudo
     CDS             6877..7456
                     /locus_tag="OO339_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         2021 a         1931 c         1820 g         1684 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acaccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca ttgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggctca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaaat agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgttt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 cataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct
     6061 aatcagcgtt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattccctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     6901 gaggcatgga ccatcatcaa gcccctgtta cctcctgaac ccgccacacc acgggccgga
     6961 cgcccatggg ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt atgttccagt
     7021 gcaccatggc gcgatttacc cgaacgatat ggggcatgga aaactgttta taaccgcttt
     7081 aaccgatggt caaagtcagg cgtgattaat attattttca acaggttgct ttcgctactt
     7141 gatgcaaacg gctttattga ctggtctgcc accgcgctgg atggcagtaa tatccgggcg
     7201 ctgaaatgtg ccgccggtgc tcaaaaaaac atcccgatat cgacggagat aatgggccgg
     7261 gtcgctctcg cggcggtttt ggcaccaaaa tccatctggc aacagacgga agtggcctcc
     7321 cgttaaatat cgtgctaagc cccggacaag ctcatgaaag ccagttcgcg caacgacttc
     7381 tggacgggat tggcgttcag cgtcagaacg gcagcatgaa acgccgtgga catgcggtac
     7441 tggctgataa agcgta
//
LOCUS       DAJTRH010000153         7369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTRH010000153 DAJTRH010000000
VERSION     DAJTRH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="OO339_003732"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="OO339_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="OO339_003733"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="OO339_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5928194.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="OO339_003734"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="OO339_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="OO339_003735"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="OO339_003735"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5928195.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDKIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="OO339_003736"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="OO339_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5928196.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="OO339_003737"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="OO339_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5928197.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="OO339_003738"
     CDS             complement(6512..7204)
                     /locus_tag="OO339_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5928198.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1863 a         1779 c         1946 g         1781 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aaaatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgg
//
LOCUS       DAJTRH010000154         7247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTRH010000154 DAJTRH010000000
VERSION     DAJTRH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..260
                     /locus_tag="OO339_003739"
     CDS             <1..260
                     /locus_tag="OO339_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5928199.1"
                     /translation="PAVLAAPGSCERIQSDISQRIINNGVPESSFTLSIVPNDQVDQP
                     DSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            459..779
                     /locus_tag="OO339_003740"
     CDS             459..779
                     /locus_tag="OO339_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5928200.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            1339..1917
                     /locus_tag="OO339_003741"
                     /pseudo
     CDS             1339..1917
                     /locus_tag="OO339_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(2603..3592)
                     /locus_tag="OO339_003742"
     CDS             complement(2603..3592)
                     /locus_tag="OO339_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928201.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(3677..4165)
                     /locus_tag="OO339_003743"
     CDS             complement(3677..4165)
                     /locus_tag="OO339_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928202.1"
                     /translation="MMFNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLSG
                     LPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAER
                     MREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWMD
                     SL"
     gene            complement(4486..4986)
                     /locus_tag="OO339_003744"
     CDS             complement(4486..4986)
                     /locus_tag="OO339_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928203.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(4976..5491)
                     /locus_tag="OO339_003745"
     CDS             complement(4976..5491)
                     /locus_tag="OO339_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928204.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(5922..6290)
                     /locus_tag="OO339_003746"
     CDS             complement(5922..6290)
                     /locus_tag="OO339_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928205.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(6702..>7247)
                     /locus_tag="OO339_003747"
     CDS             complement(6702..>7247)
                     /locus_tag="OO339_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5928206.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         1802 a         1865 c         1876 g         1704 t
ORIGIN      
        1 cacccgcggt actcgccgcg ccgggatctt gtgagcgcat acagagcgat atatcacagc
       61 gcattatcaa taatggcgta cccgaaagca gcttcacgtt aagtattgta cccaatgacc
      121 aggttgatca gcctgattcc caggtcgtcg ggcattgcgc taatgatacg cataaaattc
      181 tctacaaccg caccaccagc ggtaacgtct ctgctcccgc gcagtctacc caggatggcg
      241 cgcctgccga accgcagtaa cacattatcg actgaacgcc ggatatgaca catccggcga
      301 tttgaacata caacataatc tcaccttatt actcataccc ttctattgat gtggattaat
      361 aattcttaac ccaaaatggg tagactccct ctattgttag cgcgctaaat attcaatgta
      421 taaacattta tataacaata aagaacaggg agtgagttat gtccaaaaat gaccgaatgg
      481 tgggcatcag ccgcagaaca ctcgttaaat caaccgcgac aggttctctg gcgctggctg
      541 caggcggttt ttctttaccg ttcaccctgc gcagtgcagc ggcagcggta caacaggcca
      601 gcgaaaaagt gatctggggt tcctgttccg tcaactgtgg tagccgctgt gcacttcgtc
      661 tacatgttaa agataatgaa gtgacctggg tggaaactga caataccggc agcgatgagt
      721 acggcaacca tcagggtacg cgcctgtttg cgcggtcgct ccatccgccg gcgtattaat
      781 catcccgatc gcttgaatga cccaatgaaa cgcgtgggta aacgcggtga aggcaaattc
      841 gaacggatta gctgggatga agccctggat actatcgcca gtagcctgaa aaaaaccgtc
      901 gaacaatatg gcaatgaagc tgtatatatt cagtactctt cggggattgt tggcggcaat
      961 atgacccgct cttcgccatc agcctcggcg gtcaaacgcc tgatgaactg ctacggcggt
     1021 tcactcaatc agtatggctc ctacagcacc gcgcaaattt cctgtgccat gccctacacc
     1081 tacggcagta atgatggcaa cagcaccacg gatattgaaa acagcaagct ggtcgtgatg
     1141 tttggtaaca acccggcaga aacccgcatg agcggtggtg gcatcactta tcttcttgaa
     1201 aaagcgcgcg agaaatcgaa cgccaaaatg attgttatcg atccgcgata taccgatacg
     1261 gctgccggtc gtgaagagga atggctccct attcgcccgg gcaccgatgc cgcgctggtt
     1321 gcgggtattg cctgggtatt gattaacgaa aatctcgttg atcaaccttt tctcgataaa
     1381 tactgcgtcg gctatgacga aaaaacctta cccgcagatg cacccaaaaa tggtcactat
     1441 aaagcctata ttcttggtga aggtgacgat aaaacagcaa aaacgccgca gtgggcttcg
     1501 caaattaccg gtatcccggt agaccgtatc atcaaactgg cgcgtgaaat tggcacaaca
     1561 aaacccgctt atatctgcta gggctggggg ccgcaacgcc aggcaaacgg cgagctgact
     1621 gcacgcgcta ttgctatgtt acctattttg acgggcaatg tcggcatcag cggcggaaat
     1681 agtggcgcgc gtgaatcgac ctataccatt acgatagaac gcctaccggt gctggataat
     1741 ccggtcaaaa cgtcaatctc ctgcttcagc tggacggatg ctatcgatca tggcccgcaa
     1801 atgacggcac tccgcgacgg tgtccggggc aaagataaac tggatgtgcc cattaagttc
     1861 atctggaact acaaggactt gaagcggacc cttagtaggc ctgaggtcct gcattgagtc
     1921 aagaccttgt gtcattcttt gtgccagact tgtgtcatta gacgggatgt ctagacggct
     1981 ggtcaggacc ctccggtggc ctcggtctgg acttgaggcg ggctatgggg gaagtctgct
     2041 ggcttaatgt attagatggt cattcacatt tttctgtcag aaaccatcgg tcccactgtg
     2101 agtacaaacc taagccccca gaagcccacc cacagcctta gacagagtgt cgaatacatt
     2161 cacaccgagt cgaccggaga ggaccatata gaggcccaca gagaggaaca cgagtttata
     2221 gaccagagtc aggagggacc ttaggtcttc ggaagtcatg gacataaagc ccccccctat
     2281 cttctattgc actaccgcac tagacaacta gtacaaaaca aaggcccaga ccattggtcc
     2341 agaccgtagt tagttaatca cagtgacaag ccaccacctg aagtcctgct gatattcagt
     2401 ccagacttac ctctgctcat ggtcttacct ccctcggtct cctgaccttg ggtatcgact
     2461 acagcttgct gagcgtcatc tggaggagct acaggaggag ctacaggagg cgggaccgaa
     2521 gccccacctt gagtcccacc accacctgtc aggcccttgg cgatggacct gaagccttta
     2581 ctaatgctct tacccattgg tcttacgctt cggtcttggt gaacttcagg cgtttgacta
     2641 caggcaggac ggcagtagct gagccagtgc ccatcttgat ggtcgtagca gcttcagcga
     2701 caacgaacac tttaccgagg atttccaagt cgtgggactg agagtcgcca tcacgtgaaa
     2761 ggacgaccgt aatattctca ccgtcgtgac ggaagcagcc gaagctcagg gccgtgaagc
     2821 gtttagccag catgaataca cccttgtctt caccgttgta ataggcgatg tctgtcccca
     2881 gcatatcgac gatagcgtct tcaccggaga agccttgctt agcagccaga cgggacagtg
     2941 ctttcagttg gctctcatga gcgataatac cgtagtcaga cacctcagaa ccgaaagaag
     3001 actctgtgtt ctcatacaga gcgtccatga tagcgtctgc cttagcggtc aggctaccag
     3061 tcaggacagg aagctctacg gtcttcagca gcttagagga ttccagaacg tcgaccatgc
     3121 gagcgttgat ggtacggacc acattctcac agatgttcat agcgtcctgc tgaagtgtgc
     3181 cctgagtgaa tccggtggat gacttggcgt cccagagctt ggtctgctca gagatgagaa
     3241 tagcctgctc gattggttcg accacgattt cttgtccaga cggaacggtc acgacttcag
     3301 gtcgcttgcc accacggaca ggaacgacca cagagcggtc agccagagtg tgagcaatga
     3361 ttggggtcgg taagctgtta cgggtcggca ggacttctac agggaacggg atgttctctt
     3421 gagcgtcttt aagtacaccg ttcagcatag ccttaaccgt cttagctaac tggagggcat
     3481 agacctgcgg gtcagcgtta gtcggcagac cttcattgac ctgaatgttg tcggtatcgg
     3541 tgatgcgtac tggctcaatg ccaaactgga aagcgtcttt aatcgtagtc ataataatca
     3601 atctcctaaa ttagtttaaa tactgtgagt cctcaaaact gaggagtccc taacatcggg
     3661 tcagggactg tgtgttttac aagctgtcca tccagttacc gcctgactca aggtcagcga
     3721 caatagcggc acgggagcga ccagcggaat gggtagcact gcggaggctc tcaccatagg
     3781 tccggcgttc agctttgacc tgttcgcggc gctgtagctc tttctgccac tctgcatcgg
     3841 tcatgttctc ttcacgcata cgctcagcct gaagtcgttg ctctttagcg atacgctcag
     3901 cttcagctcg tttgttcatc tggcggcgac cataggcggt catcaagtca ttactgaaga
     3961 agttgccaat ggtcacgcca gcttcagcca gagcttccag taactccatt gcctcgcggc
     4021 tggtagacgg caggcctgac aggtagcccg gaatgagggt cttgatagcg agcgacagcg
     4081 gatagggctg accgtccaga ctcacagtgt tttcataaag gttgatgtcg gtgactgaca
     4141 gcttccagtt gttgttgaac atcataggta attctcctct tgagagttga gactatcggt
     4201 atcgcagacg cagtagcgcc tacgggtctc cccgcatcgg tcatctcaaa aggtttaaaa
     4261 ttaggtttgg tatggtcagg actgcttagc aaaggctcgg cgtaactgat tgtctaaatc
     4321 acggtccacc agcatagcta tcagctcgtc tcgactgtag taagggcgct cagggtcttc
     4381 gactccaagt ctcagcagag acagcagatg acgtgtgggc aggccacggt aacgaagggt
     4441 cgctttgccg ataggtgtac gagggtcaat catagccatg attacttacc tccactcaca
     4501 gcgaccagcg gtcacagctt gatagtctga atactcagcc tgtgagactt cacgtccggc
     4561 gatgtagagg cctgtgccat gacgctggat gaattccttg gtctccggct tgatgtcctc
     4621 agcagacgct acagcagact gcacggtggc gctggaggtc atagacacga aggctcgggt
     4681 tactgccagt ccgtacccaa ggtccaacca ccgaccgaac tctatagcgg ctactgggtc
     4741 agccagccac gaggtcccta agtgacccat gttaatcttt ttaacaaacc cgttttctgg
     4801 gttggttaga cttctaccag ccttggtcct catgaagcga gcaacctcgg tactacggtt
     4861 atcttctacc ttaccaatct gaatgcctac ccggcgtatc tcatccagtc gaaacaatcc
     4921 gtcctgactg gtgatgtcct gaccgaagat atgaatggtg tattcctgct tattgtcaga
     4981 agtcataaga ggcacctccc gccagagctt cggcaaagga cttgaagcgc tgttcttggt
     5041 cagctttcgc ctgaaggcgt tcggcatcag gtgggaccag cttatcaaag gtgccgtttt
     5101 cgtaatcttc agttaggacc tgaaggcggc gggaccattt gctctccacc tgcatcatcg
     5161 cgttagagat gtgcttcaag tcggcatcag agcgtgacgc tatccaatcc atctcttctg
     5221 agtcaccctc acaggtgtaa ggattgttga aatagaagat gtcctgcagg ccacgcttca
     5281 gctcttcctc ggtgtgtgtc tggaggtatg actggatagg ctccgcgtac atctcccgga
     5341 ttttctcata gtcccagtct tccgcctggg cataccatcg ggataacttc agcaggtcta
     5401 agttatagcg acagcaatcg acactggact ctgcattggt cagcaggatg attaagaact
     5461 ggcggttagt tactggtaat tcggtattca tacaaagatg ctcctttggt aaagcacttt
     5521 ctgtctccac atggagtccg caccattcag acgcatccta aacggaccca tcagaaagtg
     5581 cttaacgaaa gtgaatggtt aaatgttgac ggtctggagt gaccatgttc tccatgtacg
     5641 tataaaggtg cgaccgcatg tcaggacgag ctgtaaggac tccagatggt gagcggtgat
     5701 agacttaagc caatgtagtg agtagacgag ctactaaccg agacgactaa agaacgctaa
     5761 cgagctggag gtcgatacag gaggtctgtc agaggcggtc acgggccttg attacttagg
     5821 agagatactt aatgtcggtt gaccagatgc tcgcatgttc tgacgcatca gactcagagg
     5881 cggaggctgg tctccctata gtgatagttt agggtccgta atcatgaacc agctttactg
     5941 gcgtggtact ggacggattt actggcgctg acgacggtag catagacgta gcgagcgtag
     6001 cgagttgaaa ccataggttc acccaaggca acacgcttac ggctcaggga ctcctgcaca
     6061 gcgcttacag ttaatgtgtc gtgcgttagt agttgggtca gaatcaagta tgggctgaca
     6121 ggcttggtat tgcttgccat tgattcgttc aggcgaccag caccagtaag gacatcatcc
     6181 agccaaccac tcaggtcgcc ctgaccatcg aatgacagca ggtccccgca gtgctgctgc
     6241 aagtaggctt tggtgaacgt gttgtcgtga gttgcgattg gtcgtgccat agagtatctc
     6301 ctgtagttga attagttggt tcccctatag tgataatcaa gtccatcact ggtgatgcat
     6361 acagtagcgc gacaggatgt gactcacagg ttgaaccgaa tgtcaaggac taaagacgcc
     6421 tcaggaatta aggaagtata tataaagaga gaccagaggc gactcacagt taaacaggaa
     6481 tgtcaagagg agaccatagg aatttggagg gactggagtt taggacgcag tttaaataag
     6541 accacagaca gacttagagt cggacagccg aaggcggacc agtgggccta tagtcaggag
     6601 ggattatgat tattatcacc ctccctaact tcagtcagac ttcagtgacc caaggtctca
     6661 accatcaagc cgtatatggt ctccaccttg ggtctcccta cttaaagtaa agctgtctta
     6721 agcacatcag ccagcctacc cacagcgtgt cctttcccgt acaggtcata cgtaattgac
     6781 tgactgctgt gacctaagat gccttgagcg tccaccagat tgactccacg ctctttcatt
     6841 cgggtcgcca gccaatgtct cagactgtgg agagagacat cctgagagtc tcctagggcc
     6901 tcagtcagga cccttgagtt aaaccagcta gagtaggcgc tcggagtcat cccgaagaga
     6961 ggaccctcat caggctgcat gtctacccac gacaggaagg acgtcaggtc aaacccataa
     7021 gcgccatcgg ttaacggcac aagtcggact gaatgtttgt tcttcacaga cttcccgtcc
     7081 ccatcttcat tgatgtcgat acagaccagt ccattgtcca aggttacaat gtcacgttta
     7141 gtcaggtggc agacctcagc agaccgagcg ccagtgacca cagacaccag agacgcccat
     7201 ctacgggcct cagaggtcgt gtgagccgtg tgtcgtgtga actgata
//
LOCUS       DAJTRH010000155         7226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTRH010000155 DAJTRH010000000
VERSION     DAJTRH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..1003
                     /gene="ydfJ"
                     /locus_tag="OO339_003748"
     CDS             <1..1003
                     /gene="ydfJ"
                     /locus_tag="OO339_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5928207.1"
                     /translation="TLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCG
                     TLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDS
                     NQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDA
                     LMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMV
                     ALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCT
                     MTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            1092..2552
                     /locus_tag="OO339_003749"
     CDS             1092..2552
                     /locus_tag="OO339_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5928208.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2588..2791)
                     /gene="ydfZ"
                     /locus_tag="OO339_003750"
     CDS             complement(2588..2791)
                     /gene="ydfZ"
                     /locus_tag="OO339_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5928209.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2969..3655)
                     /gene="ydfH"
                     /locus_tag="OO339_003751"
     CDS             complement(2969..3655)
                     /gene="ydfH"
                     /locus_tag="OO339_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5928210.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3744..4490)
                     /gene="ydfG"
                     /locus_tag="OO339_003752"
     CDS             complement(3744..4490)
                     /gene="ydfG"
                     /locus_tag="OO339_003752"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5928211.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4627..6672
                     /gene="dcp"
                     /locus_tag="OO339_003753"
     CDS             4627..6672
                     /gene="dcp"
                     /locus_tag="OO339_003753"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5928212.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6717..7214)
                     /locus_tag="OO339_003754"
     CDS             complement(6717..7214)
                     /locus_tag="OO339_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5928213.1"
                     /translation="MVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGGKLASALC
                     AAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAIERADADV
                     SIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAVRFALAQL
                     LQLLL"
BASE COUNT         1783 a         1716 c         1860 g         1867 t
ORIGIN      
        1 gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg ccggtacgat
       61 gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg tggctatggg
      121 aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct tcattctcag
      181 taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg ttgtcgtgat
      241 ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg agaaggttaa
      301 cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc agagcaaatc
      361 cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt taattcagac
      421 tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc caacagatgc
      481 attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt ggttatccga
      541 taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg tgctggcgtg
      601 gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta tggttgcact
      661 gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca ttaccatggc
      721 agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag aaattggtgg
      781 tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca tgacggaatc
      841 ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa tctctgcgct
      901 gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg cggaagatca
      961 accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca atcccttctc
     1021 ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat atttaaattt
     1081 aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct gtttatgatc
     1141 gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac cgtgcgcatc
     1201 agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga tattatgagg
     1261 tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat aatctttata
     1321 ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc gttaaaaaag
     1381 ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt gaaccgcaaa
     1441 tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg gcgaccggac
     1501 agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac cagccgaaaa
     1561 cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga cttcccgcat
     1621 ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt gacgttgtca
     1681 cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat aacgtgactt
     1741 tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg ctggcgaaaa
     1801 tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct ttccgccagt
     1861 gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg ggagccgaac
     1921 tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac ggcagtcatt
     1981 cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac tgtatggaag
     2041 atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg ccgacgctga
     2101 aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc tatagcaacc
     2161 cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa ttgccacagc
     2221 ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat ctgctggcgc
     2281 tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga aatccgatag
     2341 aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt gccgaaggga
     2401 aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat ttgccagaca
     2461 atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg catggcgcga
     2521 aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat cggcggcatt
     2581 cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc tcttcacaac
     2641 cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct tcagtatcaa
     2701 tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg ccagtggtga
     2761 ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg atttacagtc
     2821 gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt ttttgttttt
     2881 gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg aaaagcctct
     2941 catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg ctgttttcct
     3001 ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt gcacgttcta
     3061 ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg cgtaacagca
     3121 tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg gtcgctttca
     3181 ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg agttgatgga
     3241 agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca atgcgttgct
     3301 ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc gccgcccgac
     3361 gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc tgggccatgg
     3421 aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg ttttccgcca
     3481 gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca gaaacttctt
     3541 tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga cgaagaatac
     3601 gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg gtcattcgtt
     3661 cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa gcaataaccc
     3721 ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc atagctttgg
     3781 gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt tgacacccac
     3841 cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata ggttttttcc
     3901 gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc caccagaccc
     3961 ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag attcaggcta
     4021 aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata cggccagctg
     4081 cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat acccggtaag
     4141 acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt ttcccagtct
     4201 tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt atttaccagg
     4261 atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat agcggcgcgg
     4321 ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt taactcctgc
     4381 aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat aaaacgacga
     4441 gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat ctcactgttc
     4501 ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg taatactggt
     4561 ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg cgttcaggag
     4621 aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta tctggctcct
     4681 cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg aatgcaacaa
     4741 aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt caacaatact
     4801 attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt cttttttgcg
     4861 atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt ttccgctgaa
     4921 ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg ggtagatgct
     4981 gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct ggtggaggtt
     5041 attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa agcaaaatta
     5101 aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg gttacttgca
     5161 gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg aatgagtgag
     5221 caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa caaatggctg
     5281 attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga tcgtgcgacg
     5341 cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc caatgatacc
     5401 cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact gcttggtttt
     5461 cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga agcagcactc
     5521 aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga attagcctcc
     5581 atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg ggactgggca
     5641 ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca gctcaagcca
     5701 tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa tcagctcttc
     5761 ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt tcgtgtgtgg
     5821 gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt cttcgcccgt
     5881 gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac gcttaatgaa
     5941 acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg tgagcctgcg
     6001 ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac gctgcacggc
     6061 ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg tgattttgtc
     6121 gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt cgctcgctac
     6181 gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa aatgcgtaat
     6241 gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc acttctcgat
     6301 atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga ttttgaattg
     6361 cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta tcgcagcagt
     6421 tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta tctgtggacg
     6481 caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt aacgcgtgaa
     6541 aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga tctggaacgc
     6601 ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca acatcgtggc
     6661 ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca gcctggttat
     6721 agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa taccgtttcg
     6781 cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg ccacgcgaac
     6841 cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta gccggtaatg
     6901 gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc tgctgccact
     6961 ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag gattttcatc
     7021 tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt atcttcagct
     7081 gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt taagcgtaat
     7141 tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt gtcggtatct
     7201 gcgagttgta caattttatc tttatt
//
LOCUS       DAJTRH010000156         6963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTRH010000156 DAJTRH010000000
VERSION     DAJTRH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(365..946)
                     /locus_tag="OO339_003755"
     CDS             complement(365..946)
                     /locus_tag="OO339_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928214.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(993..4850)
                     /gene="sigA"
                     /locus_tag="OO339_003756"
     CDS             complement(993..4850)
                     /gene="sigA"
                     /locus_tag="OO339_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5928215.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGILTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5211..6215)
                     /locus_tag="OO339_003757"
                     /pseudo
     CDS             complement(5211..6215)
                     /locus_tag="OO339_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6200..6577
                     /locus_tag="OO339_003758"
                     /pseudo
     CDS             6200..6577
                     /locus_tag="OO339_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6616..>6963)
                     /locus_tag="OO339_003759"
     CDS             complement(6616..>6963)
                     /locus_tag="OO339_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5928216.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1734 a         1567 c         1573 g         2089 t
ORIGIN      
        1 catcacctgc tattaatccc cgagtcagta cttcgcggcc tgacgcgatc accctactca
       61 gtacttcaga gcccgcctcc ggtacctgcc tggcgatcgc ccctgtgcaa caaaataaat
      121 caaccgttat caaaccacga tcgttcgtat gcgccgccga tgacgcgcgc ctgaaaaacg
      181 cgtcaatatg agttccccgg aagcttacga taatacgctt atgaacatta caacacgctc
      241 atgcggaacc tggatggggg aaggaacggt gaagctgtta gcgaatgacc ggcgtttata
      301 caaacgccgg tcatgatgaa tgatatttca gaatatacct cgggctaaaa taccgggaca
      361 gagatcaggc gtgattaatc tgcagcattt ttaataacag aagtactgag cttactttac
      421 ctttgatgat gtaatagtta tttccgtcgg tactcatttt tccctgaaat aatgtcacgg
      481 atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt catattttta atgagctgag
      541 atccgggtgg caaggggtat ttgctgtcag atactgggcg actgagtctg atgtggcgcg
      601 tatacagccc gttttcgtac agggtggcct ctgttatggt gtcagtgacc agcatgtggg
      661 agacaatgct gtattctttt ttaaagcacc caccagaaac ggtgattgta atgataatgg
      721 tcttctcgat cgagatgtaa atacccgttt cgttatactg tccgtatcgg aaccatgaga
      781 actcagttgc attccagcca tgctccagaa tatgtgcatt tttttgcctg atagtttctt
      841 tttttattcg ctcatgttta aggcataaca ggcagataaa taatgtcaga aaaagaaagc
      901 taataatgag agcaagagcg atatagtctg tcacagggta atacatattc tctccggtag
      961 aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag agtaacggaa gttggcgttg
     1021 atcgcgttat ccacgttgta tttaccaaat gccgatttct caaactcaag accgaagcga
     1081 agattatcgc gaatttcggc gttgagacca acattcatga gaatacgacc gtctttttca
     1141 cctttgatac gtttctcacc ggacgcatca cgcagtacgg tttcaccgtt ggcaaacagg
     1201 tcaaactggt agccaaggcc ggcgcgggct gtgactttcc agtccttacc ggagaaggat
     1261 ttacccacat caacaccggt acgcccaatc agcggattaa agtccttatc cttcatggtc
     1321 acgctcattc cgcgatcctg ccagtcgaac cgtttacctg atacggcccc ataaaccagt
     1381 tctgcctgcg gctcaatcca ggagtcttct gttacatgat agcggtagcc tgcttcagca
     1441 cccacataca aggaatgaga actgtagtct tttgttccga gtccagcaaa ggtcgccgag
     1501 tactcattat catggtgaac atacttacta atcaggtcga tataggcacc agagtcaaat
     1561 atagcggaag catacagacc tgccccgacg gacttcgtct tgccactgaa tgcattactg
     1621 ctggcatgac tgtcggtata cgtcatagtc agaccagtga aaaggtcaag tccatccagc
     1681 tcatgttttt tatccacacc aatctgcaca tgggtgtagt tgtcactgta tccaccagaa
     1741 gctgaacctg ctccgctcat gatgcgggcc catgcaccgg cctcgccatt ggtgtcacgc
     1801 agatccccca ttcgtttatt caggttgttg acttctgtca ggaaggcttt gtaattaccg
     1861 gacatatacg attttgctgc accggaagat gctgtatttt cagccacctt atatccggtc
     1921 agggtccacg tggtatgttc ctgatcctct ttcgtgctga tcacaggaga tatgttgctg
     1981 aaacctatgg cctgagtact ggctttaaat atctctttat tgcttccttt tggggctgtt
     2041 atcaggtcaa cgtttaatcc tgagttatta tttcccactt tctgtatatc gaccgacaga
     2101 ctgttattgc ttccggttaa ctgattttta acaatgagtt tatccgattg atttacagac
     2161 tggctcagat cagcccgcat aatcacagca gtattagtgg catccagtgt gtttactgtc
     2221 acagtatgga aacccgaatt ctctatgtta ctaaacaaca aaagactgtc gcgggttttt
     2281 aatgattgca gggctgactg tccgtttact ctccataagg tgttatacat gctggctgag
     2341 gctgcaggag catcaatacc tccggtataa gaagtatcaa agccacccag caaacctgta
     2401 gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt tatactggtc aacaccaaac
     2461 agaatagatg cttttttgtc tgattttata ttaccattga cccaggcata attttgagcc
     2521 tttaacgtgg caccatcttc tgtcatatta tatccatctg acatatagat tgccgggctg
     2581 tatttattat tttctgtagg ggtgcctgtt aatgtcaggg ttccggccag ttcaatagct
     2641 ttatctacat acatttttcc ctgggcggta acagtagctc ctttgttgat aatcaggtct
     2701 gtattaatta aggataatgc accagtcata gtaacagttg atttatcacc aacagttatt
     2761 ttgctgtttt gagcactcag actgccgttg aaactacttt cgcctgctga atccccggag
     2821 cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt ttcttcctga gaacatgtca
     2881 atgtatgcag ttttatttcc cagaacaatt tgagagtttt tagcctctat gtgtcctttt
     2941 acattagcat cccggcctat actcagggtt gcgttttcta aatttaattt ccggaaatta
     3001 aatttacgac tttcccagtc gggctgggaa aaagaggcta tctgattgtt ggacttatat
     3061 tccgtattgt aacgtttatt tacagtgctt tcctggtcct ttatggcagc agaatagtct
     3121 ttttgacata aaaaaggaat tggacagttg ttattgcctt ctttaaatat ggcatgttct
     3181 gtcgcatgtc cttgtaatgt taactgtgca ttccggacag agatatcgtt tttgatatcg
     3241 actgagccat caaagatcag attggcatta ttgttatgtt gctgggtagt attgataata
     3301 tgatttatgc ttatattacc tgatacattc ccatggtaca tatatgcatc agtattagta
     3361 agtgataatg ttgattgctt cacgtttgag ttcgttacgg ttgttcccgc atctgttgct
     3421 gcgattttct gaaatgactg gtcatagccg tttagatcca gtgttccgcc atttctggta
     3481 aaatagattt cgccattacc gctttcgctt aaagcgtctt tggcatttat ttttaccgta
     3541 cctttacctc cggccatgta aattttattg aaggcttttt cagcactaag gatgacagtc
     3601 ccattaccta ttttaaggtt atttccctgt gctattttta catccagagt accactacct
     3661 attttatgca gattatctcc ggcaacgccc ttaacattcc attcaacggt ggtattatta
     3721 tcaatatcta tcccggcacc tttataagac ttatcttttc cgtacacttt atatgtctgg
     3781 ttcttatcga atataagccc gccaatgcca agatcggtat cctgcttgag cgttaatatc
     3841 cctcctcctg agacagacag gtccttatct tttataatcg atgataactc ttgtttttta
     3901 ccatccagct gcatagtgtt tgtgaaattc ttttttaaat tgtttaccag aatattatta
     3961 aataacgagt agtttgtcca ggctatattg gtactactga taaaaccaac tgtactggta
     4021 acaccatgga aaacccattt ttgttgttct ttatcatata gataagaacc agaaccacta
     4081 tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg tataaatgta actcttgttt
     4141 ttccagtcca gattaaaaag acttgcgctt aacaagtcgg gattatagtt ttgtccaaac
     4201 agatattgtt tgccgtttaa tatcgttgag gttgttccgg cacctgctct gaagccaatt
     4261 atttgttcct taccgttgta gtttacgcca tatcttttca tcgcatcttc gggagaaagt
     4321 gagaagtcgg cactctccgt aacccctgtt gtttccacga cgaatttatt cagacgatga
     4381 accgaaaagt cgccagatga tactctgtta cttgctttat atgctgtctg atcccatgac
     4441 tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg cagtaacact gtacgcacct
     4501 ccaattgatg ttgttgcacc tttgttggaa acagcagaaa aatcaggtac aggtagttct
     4561 ggaaaatgaa atatctttcc atctttcatc attaattgaa catttgttgc acccggtctg
     4621 aactctcctc tgttttgagc aaggtcaaga taatcgcggg tccatatatt atttatatcc
     4681 agcattgctg caaatgctgt ttcgccataa cttccatata ttgaggataa aataataccg
     4741 aggataactt tctttcttga tgtaccgaca ctaactttcc gggtcagttc agaaacagca
     4801 actaatccac ctgtaatatg actatatttc agtgaataaa ttttattcat acgtgaactc
     4861 cgatatttct gagttgtggt tatgggatag catttaatta caacggggaa ctatcagtca
     4921 tggtacgtaa ctgataaggg tataatgata atcactgtca gttatatttg tatgacagat
     4981 gtaacaaaat gaaaaaaaag ataagcacaa gtgattgatt ggttagatat tgatcacttg
     5041 gtgtttgtat tttgaatttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     5101 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     5161 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     5221 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     5281 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     5341 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     5401 gtgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     5461 tccacatcaa atgcagtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     5521 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacgggag
     5581 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     5641 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     5701 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     5761 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     5821 tcagtgatca gtgccgtgcg ggcacaggcc tgtgatgtca ggcggctttt gtccacctcc
     5881 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     5941 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     6001 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     6061 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
     6121 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     6181 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     6241 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     6301 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     6361 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     6421 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     6481 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     6541 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     6601 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     6661 aatatcccac ataaatccag tcaactcacg agccaccgcc gtaatggcta cttgtgattt
     6721 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     6781 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     6841 ttttgcaggt gtacgataag accaggcggc ttcgtaaagc agacgtctca gttcgctgtt
     6901 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     6961 cag
//
LOCUS       DAJTRH010000157         6803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTRH010000157 DAJTRH010000000
VERSION     DAJTRH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            41..1531
                     /locus_tag="OO339_003760"
     CDS             41..1531
                     /locus_tag="OO339_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5928217.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1583..2740
                     /locus_tag="OO339_003761"
     CDS             1583..2740
                     /locus_tag="OO339_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5928218.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2706..2828)
                     /locus_tag="OO339_003762"
     CDS             complement(2706..2828)
                     /locus_tag="OO339_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928219.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3031..4716
                     /locus_tag="OO339_003763"
                     /pseudo
     CDS             3031..4716
                     /locus_tag="OO339_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4754..>6803
                     /locus_tag="OO339_003764"
     CDS             4754..>6803
                     /locus_tag="OO339_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5928220.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     G"
BASE COUNT         2007 a         1481 c         1433 g         1882 t
ORIGIN      
        1 atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc ttggttatgg
       61 acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc gtgaagttgt
      121 cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat caacgccgac
      181 gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg cacacggtgt
      241 ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct ttggtgtcta
      301 ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc ctgaattatt
      361 ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa aaatcagtaa
      421 tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca ccacattttc
      481 tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat tccacgctgc
      541 aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg tccccgcaaa
      601 aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc gtgccaaaac
      661 acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac caaatgataa
      721 tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag cagataacta
      781 ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac aactgaagaa
      841 aaacggacag tatgacaata caattattct ctttacctcc gataatggtg cggttatcga
      901 tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct atcctggcgg
      961 tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta attatgacaa
     1021 gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata tcagcattcc
     1081 aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata agaaacaagg
     1141 cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg atgaggaaaa
     1201 tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg attacccgca
     1261 taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata acgattattc
     1321 gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg atctacagca
     1381 aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag gcgtggtaag
     1441 agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga agtttaacaa
     1501 tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg atttttccgc
     1561 cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt ttcaatgtaa
     1621 tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc atgaaaaatg
     1681 gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt ctggcaatca
     1741 ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt ttttccgtaa
     1801 agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata cattacaaac
     1861 gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg aatttctggt
     1921 tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca gtaattcagg
     1981 taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat atcaagtaga
     2041 gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg aggtttatca
     2101 ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag aaaccgggac
     2161 gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg atttttctgt
     2221 gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg ttaaaaacga
     2281 tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt tggggaatgg
     2341 gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg aaagtaatgg
     2401 agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa acattaataa
     2461 atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac ggatttcagc
     2521 gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta agcatcgtat
     2581 tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa tccttttttc
     2641 aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag taccgcttta
     2701 ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc ttttaactcc
     2761 attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc tgtcgggtgt
     2821 ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg tcagggcatt
     2881 agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa aatttctata
     2941 tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt gccatagata
     3001 atcattggat ggcacaatca attttcgcta atgataacca ttcccattac gttttgtatg
     3061 ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa taataaaact
     3121 tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa aattcaggtc
     3181 tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga aaccgataag
     3241 ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt aatatccgca
     3301 aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt aacagattac
     3361 taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta cggcgagcat
     3421 aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact gatcaacaaa
     3481 acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac ctttaccgtt
     3541 attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga atggaatatc
     3601 caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt atttacccat
     3661 aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga agcgacattt
     3721 cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag caatgctgag
     3781 tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa ctggcatcgt
     3841 ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg ctcgcttagc
     3901 gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa tctgggcgga
     3961 ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg gtttatttat
     4021 aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga gttccatcaa
     4081 ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt acaagtggct
     4141 aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa ctttcatgtt
     4201 tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac cacactgctt
     4261 aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc tgctgacagt
     4321 tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat tatttgtaaa
     4381 gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca ggttggtctg
     4441 ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct ttccagcggc
     4501 gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg gatatttctt
     4561 gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg tttagtgcgt
     4621 gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt ctggaacctg
     4681 gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc ctgctgtacg
     4741 gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg gcgctgatgt
     4801 ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga tgacggtcta
     4861 tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa gcatcgagcg
     4921 tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg cggtacgcat
     4981 ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga aaatctctat
     5041 tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg caactaataa
     5101 cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg ggatgtcaca
     5161 ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca gttttgtgcc
     5221 ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac gcttcaacgc
     5281 tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt taacatcgca
     5341 tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta cctatttctc
     5401 tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg ctgataactt
     5461 tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg attatgtttc
     5521 gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata ctgcattgag
     5581 caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa aatataccgg
     5641 ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa tgggtaataa
     5701 atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac tacgtaccac
     5761 cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct ggtacaccga
     5821 actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat taggacacat
     5881 ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa aattcgccgt
     5941 gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg atgcatggac
     6001 tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga aaactaacca
     6061 taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc tgtatatggc
     6121 ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg actatgacaa
     6181 ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata tttttgctga
     6241 tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata ttcttgatat
     6301 gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata aaaccctgac
     6361 gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat tgcagcaaaa
     6421 aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca gcaaaagcag
     6481 tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg gcaaaaccaa
     6541 aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc gccaggtaga
     6601 tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt cgttaaacaa
     6661 tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta atctggtctc
     6721 ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct taaacatgga
     6781 tttcacgcat caaccgagcg ggt
//
LOCUS       DAJTRH010000158         6790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTRH010000158 DAJTRH010000000
VERSION     DAJTRH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            16..762
                     /gene="glnH"
                     /locus_tag="OO339_003765"
     CDS             16..762
                     /gene="glnH"
                     /locus_tag="OO339_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5928221.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            901..1560
                     /gene="glnP"
                     /locus_tag="OO339_003766"
     CDS             901..1560
                     /gene="glnP"
                     /locus_tag="OO339_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5928222.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            1557..2279
                     /gene="glnQ"
                     /locus_tag="OO339_003767"
     CDS             1557..2279
                     /gene="glnQ"
                     /locus_tag="OO339_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5928223.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            2540..4764
                     /gene="ybiO"
                     /locus_tag="OO339_003768"
                     /pseudo
     CDS             2540..4764
                     /gene="ybiO"
                     /locus_tag="OO339_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(4761..5687)
                     /gene="rlmF"
                     /locus_tag="OO339_003769"
     CDS             complement(4761..5687)
                     /gene="rlmF"
                     /locus_tag="OO339_003769"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5928224.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            5963..6223
                     /locus_tag="OO339_003770"
     CDS             5963..6223
                     /locus_tag="OO339_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5928225.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            6488..>6790
                     /locus_tag="OO339_003771"
     CDS             6488..>6790
                     /locus_tag="OO339_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5928226.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT         1608 a         1767 c         1745 g         1670 t
ORIGIN      
        1 aaaaaaggaa atgctatgaa gtctgtatta aaagtttcac tggctgcact gaccctggct
       61 tttgcggttt cttctcatgc cgcggataaa aaattagttg tcgcgacgga taccgccttc
      121 gttccgtttg aatttaaaca gggcgataaa tatgtgggct ttgacgttga tctgtgggct
      181 gccatcgcta aagagctgaa gctggattac gaactgaagc cgatggattt cagtgggatc
      241 attccggcac tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac catcaccgac
      301 gagcgtaaaa aagcgatcga tttctctgac ggctactaca aaagcggcct gttagtgatg
      361 gtgaaagcta acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa agtggttgct
      421 gtgaagagcg gtactggctc cgttgattac gcgaaagcaa acatcaaaac taaagatctg
      481 cgtcagttcc cgaacatcga taacgcctat atggaactgg gcaccaaccg cgcagacgcc
      541 gttctgcacg atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa cggtcagttc
      601 aaagcggtag gtgactctct ggaagcgcag caatacggta ttgcgttccc gaaaggtagc
      661 gacgagctgc gtgacaaagt caacggcgcg ttgaaaaccc tgcgcgagaa cggaacttac
      721 aacgaaatct acaaaaaatg gttcagtact gaaccgaaat aataacgcta cacctgtaaa
      781 gcgcactggc agttccctct cccccctggg gagaggatta gggtgagggg cgcaaacccg
      841 ctccggggcc attaattacc ctgaatttga ttatttacac cacggtaaca ggaacaacat
      901 atgcagtttg actggagtgc catctggcct gccattccgc ttctgattga aggtgccaaa
      961 atgaccctgt ggatttcggt cctcggtctg gctggcggtc tggtaatcgg attgctggca
     1021 ggttttgcac gcaccttcgg tggttggata gccaaccacg tcgcgctggt ctttattgaa
     1081 gtgatccgcg gcacacctat cgtcgtccag gtgatgttta tctatttcgc cctgccgatg
     1141 gcgtttaacg acttacgcat cgacccattt actgcggcgg tggtcaccat catgatcaac
     1201 tccggcgcgt acattgcgga aatcacgcgt ggtgcggtgc tgtctatcca caaaggtttt
     1261 cgtgaagcag gactggcgct cggtctttca cgttgggaaa ccattcgcta cgtcatttta
     1321 ccgctggcac tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat cagcattaaa
     1381 gacacctcgc tgtttattgt tatcggcgtg gcggaactga cccgtcaggg gcaagaaatt
     1441 attgccggta acttccgcgc ccttgagatc tggagcgccg tggcggtgtt ctatctgatt
     1501 attaccctgg tgctgagctt tattctgcgt cgtctggaaa gaaggatgaa aatcctgtga
     1561 ttgaatttaa aaacgtctcc aagcactttg gcccaaccca ggtgctgcac aatatcgatt
     1621 tgaacattgc ccagggcgaa gtcgtggtga ttatcgggcc gtccggttcc ggtaaatcga
     1681 ccctgctgcg ctgcatcaac aaactggaag aaatcacctc cggcgatctg attgtcgatg
     1741 gcctgaaggt caacgatccg aaagttgacg agcgcctgat tcgccaggaa gcaggtatgg
     1801 tgttccagca gttttacctc ttcccgcatc tgacggcgct gggaaacgtc atgtttggtc
     1861 cgctacgcgt gcgtggcgcg aacaaagaag aggcggaaaa actggcgcgt gagctgctgg
     1921 cgaaagtcgg tctggcagaa cgtgcacatc actacccttc cgaactttct ggtggtcaac
     1981 agcagcgtgt ggcgattgcc cgcgcgctgg cggtgaagcc gaaaatgatg ctgtttgatg
     2041 aaccgacttc cgctcttgac ccggaactgc gccatgaagt gctgaaggtt atgcaggatc
     2101 tggctgaaga agggatgacg atggtgatcg tgacccacga aatcggtttt gccgagaaag
     2161 tagcttcgcg gctgatcttt atcgacaaag gccggattgc ggaagatggc aatccgcagg
     2221 tgttgatcaa gaacccgccg agccagcgct tgcaggaatt tttgcagcac gtctcttaat
     2281 aagacacatt gcctgatgcg ctacgcttat caggcctaca ggatatctgg caacttatta
     2341 aaattgcatg aacttgtagg acggataagg cgttcacgcc gcatccggca aaaaagcccg
     2401 cacgttgtca gcaacctgct taatatccct tcctcccttt cacccgaaag ggaggcacac
     2461 cagattcctc tcatttaaaa tcgcccctcc cccagcatct atacttatct ttttgctctg
     2521 ttttctcact ggaggagtca tgcggtggat cctgttcatc ctcttctgcc tgctgggcgc
     2581 acctgcccac gcggtatcca tacccggcgt tacaaccaca acgacaacgg actcaacgac
     2641 tgaaccggcc ccggaaccgg atatcgaaca aaaaaagcgg cctatggcgc actggcggat
     2701 gtgctggata acgacacctc gcgtaaagag ttgatcgacc agttgcgtac tgtcgccgcg
     2761 acgccccctg cggaaccggt cccgaagatc gtgccgccga cgctggttga agagcaaacc
     2821 gtgctgcaaa aggtcaccga agtcagccgc cattatggtg aagccctttc cgcccgcttc
     2881 gggcaacttt atcgcaatat caccggctcc ccgcataagc catttaatcc acaaaccttc
     2941 agcaatgcgc tgagccattt ttcaatgtta gcggtattag tgtttggttt ttactggctg
     3001 attcgcctgt gcgcactgcc gctatatcgc aaaatgggtc agtgggcgag gcaaaaaaat
     3061 cgtgagcgca gcaactggtt gcagcttccg gcgatgatta ttggggcgtt tattatcgac
     3121 ctgctgttac tggccctgac attatttgtc ggccaggtat taagcgacaa cctgaatgca
     3181 ggcagtcgca ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc cctgattgaa
     3241 tttttcaaag ccgtactacg cctgattttt tgcccaaacg tggcggagct gcgcccgttc
     3301 acgattcagg acgagagcgc ccgttactgg ggtcgtcgcc tgagctggtt aagcagcctg
     3361 attggctatg gcctgattgt ggccgtgccg attatctcta atcaggtgaa tgtacagata
     3421 ggtgcgctgg cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt gtacctgatc
     3481 ttccgaaata aaaaagagat tacccagcat atgctcaact ttgcggagca ttcgctggct
     3541 tttttcagcc tgtttatccg cgcctttgcg ctggtgtggc actggctggc aagcgcctat
     3601 tttatcatgc tgtttttctt ttcgttgttc gatccgggca acagtctgaa atttatgatg
     3661 ggtgcaacgg tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc cggtatgttt
     3721 tcccgctggc tggcgaaaac catcaccctc tcgccacata ctcagcgtaa ctatccggag
     3781 ctacaaaaac ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg tattctgacc
     3841 gtctgcgtgg cagtaatgtt gctgttgagc gcatggggat tgttcgattt ctggaactgg
     3901 ctgcaaaacg gcgcggggca gaaaaccgta gatatcctga tccgcatcgc actcattctt
     3961 ttcttctcgg cggttggctg gacggtgctc gccagtttga tcgaaaaccg gctggcttcg
     4021 gatattcatg gccgcccgct acccagcgcc cgtacgcgta ccctgctgac gctgttccgt
     4081 aacgcgctgg cggtgattat cagtaccatc accatcatga ttgtgttgtc ggaaatcggc
     4141 gtcaatatcg cgccattgct ggcaggtgcc ggggcattag gtctggctat ctcgtttggt
     4201 tcgcaaacgc tggtaaaaga tattatcacc ggggtattta ttcagtttga aaacggcatg
     4261 aacactggag atttggtgac tatcgggccg ttgaccggca cagtggaacg gatgtcgatt
     4321 cgctccgtgg gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg gtcttcgata
     4381 accacctttg ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta cgatgttgat
     4441 cgccatgaag atgctgataa agccaatcag gcactgaaag atgcggtaac ggaattgatg
     4501 gaaaacgaag aaattcgcgg gctgattatt ggtgaaccga actttgccgg gattgtcggc
     4561 ttaagcaata ccgcgtttac actgcgtgtt tcgttcacca cgctgccact caaacagtgg
     4621 acggtacgct ttgccctcga cagccaggtg aaaaaacatt tcgacctggc gggcgttcgc
     4681 gcgccagtgc agacttatca ggtgctgcct gctccgggcg cgaccccggc tgaaccgctg
     4741 ccgccggggg agccaacgct ttaacgttgg cgattaacaa aacggcggcg ctgctcgtcg
     4801 ttcataaagg tccaggcaat aaagcgactc tgcttttgcc cctgggccat ctcttttttt
     4861 accaccttca ccgcgcccac gtcggtcaga gcacgataca acggcggtaa gttttcacca
     4921 cgagatacca gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct ctcttcaatc
     4981 atctttttga taaagttgac ttcaccgcct tcacaccaca actcctgttg ctgaccacca
     5041 aagttcagtg catcgtcttt gttcagcccc aggttacggc gtttacgctc actacctgcc
     5101 cgcgccgcag cggcggaatc gtggaatggc gggttacaca aggtcgcgtc gtattgctcg
     5161 tttttgtgga tgatgccgtt aaaaatcgcc ccactctctt tttgccgacg cagacgaatg
     5221 gcgcggttaa gccccggatt agcactgata atcgcctgcg cactgcttaa cgcctggctg
     5281 ctggtttcac taccggtaaa acgccagcca tattcatgta cgccaatcag cggataaata
     5341 cagttcgcac caacgccgat atccagaatg ctggcattcg ctggaattgt tccgcttgct
     5401 tcagccagta aatcggcaag gtgatgaata taatccgccc ggcccggtac tggtgggcag
     5461 agaaaaccgt cggggatatc ccagttcgct acggcgtaaa aatgggccag caacgcctta
     5521 ttgagcgcct tcaccgccag cggattggca aagtctacgc tttgctcccc tgcgggtgta
     5581 agcgtgagga attgcctgag ttcaggattg acctgacaaa gcgtggcgag atcgtagcgg
     5641 ctgtgatgac ggttgcgcgg atgcaacccc ggtttctggg cggacatagc attctccttt
     5701 aagcagcggc gtaagatacc cgcataacgc gacagggtaa atatctggct gcgtttatca
     5761 ttccccaaaa ccttaacatg gctatggttt atggcaattc atcataaaaa tcagcattat
     5821 gcatgtcatt gatttgtctt attttgtaaa aatatatcgt tcaaaaaata agcatttctc
     5881 tgtcaatttt ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag gcactacccg
     5941 ttgtatttat gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg ccaccgtcct
     6001 gagcggaatc tctctcacgg cttatgccgc gcaaccgatg agtaacctgg acagcggtca
     6061 gctacggccc gccggtaccg tttcggcaac tggcgcatca aacctaagcg atctggagga
     6121 taaactggca gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta attccgcagg
     6181 cggaaatgac cagatgttag gtactgcaac catctacaaa taaccccacc aacgtaactg
     6241 cattcgcctc tggatgcagt tttcgcctgt tttgtaatca aaaaacatca aatatgaacg
     6301 caatgtaaat aaatgtattt ctttttcgcg caatgggtga tagaaaatcg catcaagtga
     6361 taatgcttat caaaattatt atcacttgca cgaacactat cacgggatta acagtggcat
     6421 cgcatccgca gagatgcttt ctcgtggcag tgaaaatttc aacatataag aaaaagtcac
     6481 ctgcaaaatg gaaaacaatc gcaatttccc tgccagacaa tttcattcgc tcacgttctt
     6541 tgccggtctt tgtattggta ttacgcctgt ggcacaggca ctcgccgccg aagggcaagc
     6601 taacgcggat gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg cgccacaaca
     6661 atctgccgat ccgaaattct cgcgtccggt agcggatact acccgcacga tgacggtgat
     6721 ttctgaacaa gtgattaaag atcagggcgc aaccaacctt accgacgcgc tcaaaaacgt
     6781 ccccggcgtg
//
LOCUS       DAJTRH010000159         6645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTRH010000159 DAJTRH010000000
VERSION     DAJTRH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="OO339_003772"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="OO339_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5928227.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="OO339_003773"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="OO339_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..6527
                     /gene="narH"
                     /locus_tag="OO339_003774"
                     /pseudo
     CDS             5646..6527
                     /gene="narH"
                     /locus_tag="OO339_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
BASE COUNT         1590 a         1742 c         1720 g         1593 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct ctatcgagta ggcagcctga
     6541 cggctgcggc ttgtcatggt ctgcaattac cgttataaaa acaggcaatg tcattgtctt
     6601 tcaggtggtt atatggcccg ttcagctaaa ccccgtaaac gtaaa
//
LOCUS       DAJTRH010000160         6631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTRH010000160 DAJTRH010000000
VERSION     DAJTRH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..610
                     /locus_tag="OO339_003775"
     CDS             <1..610
                     /locus_tag="OO339_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5928228.1"
                     /translation="PRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGG
                     QIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKQGPYEFI
                     CTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIM
                     CRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            980..1804
                     /gene="gadX"
                     /locus_tag="OO339_003776"
     CDS             980..1804
                     /gene="gadX"
                     /locus_tag="OO339_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5928229.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            2172..2900
                     /gene="gadW"
                     /locus_tag="OO339_003777"
     CDS             2172..2900
                     /gene="gadW"
                     /locus_tag="OO339_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5928230.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3263..6376)
                     /gene="mdtF"
                     /locus_tag="OO339_003778"
                     /pseudo
     CDS             complement(3263..6376)
                     /gene="mdtF"
                     /locus_tag="OO339_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(6401..>6631)
                     /locus_tag="OO339_003779"
     CDS             complement(6401..>6631)
                     /locus_tag="OO339_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HCR5928231.1"
                     /translation="THNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGD
                     RVIVSGLQRIRPGIKARAISSSQENASTESKQ"
BASE COUNT         1708 a         1584 c         1600 g         1739 t
ORIGIN      
        1 gccgcgtgtg aaatcgatca gtgcttcagg ccataaattc ggtctggctc cgctgggctg
       61 cggctgggtt atctggcgtg acgaagaagc gctgccgcag gaactggtgt tcaacgttga
      121 ctacctgggt ggtcaaattg gtacttttgc catcaacttc tcccgcccgg cgggtcaggt
      181 aattgcacag tactatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtacagaa
      241 cgcctcttac caggttgccg cttatctggc ggatgaaatc gccaaacagg ggccgtatga
      301 gttcatctgt acgggtcgcc cggacgaagg catcccggcg gtttgcttca aactgaaaga
      361 tggtgaagat ccgggataca ccctgtacga cctctctgaa cgtctgcgtc tgcgcggctg
      421 gcaggttccg gccttcactc tcggcggtga agccaccgac atcgtggtga tgcgcattat
      481 gtgtcgtcgc ggcttcgaaa tggactttgc tgaactgttg ctggaagact acaaagcctc
      541 cctgaaatat ctcagcgatc acccgaaact gcagggtatt gcccagcaga acagctttaa
      601 acacacctga taacataacg ttgtaaaaac cgaatgccca gcctttaaaa aaacagctgg
      661 gcattcggtt gcttattaat cgcaataata tattggctgt ctattcatcg tgttgataag
      721 atatatgtta tatgaatgtt tattaaatgt agcgattacc tttaatgtca cgaaaaggga
      781 ttatttgctt gctattaatt tcactgcgtt cttaaacacg tttttgtatt ataaatccag
      841 tcatcctgcc cgtagaacat cacacattat catcctgttc tcccgcttcg tttaaattta
      901 tttatcaatc aatttgactt aagagggcgg cgtgctacat taattaacag taatatgttt
      961 atgtaatatt aagtcaacta tgcaatcatt acatgggaat tgtctaattg cgtacgcaag
     1021 acataaatat attctcacca tggttaatgg tgaatatcgc tattttaatg gcggtgacct
     1081 ggtttttgcg gatgcaagcc aaattcgagt agataagtgt gttgaaaatt ttgtattagt
     1141 atcaagggat acgctttcat tatttctgcc gatgctcaag gaggaggcat taaatcttca
     1201 tgcacataaa aaaatttctt cattactcgt tcatcactgt agcagagata ttcctgtttt
     1261 tcaggaagtt gcgcaactat cgcagaataa gaatcttcgc tatgcagaaa tgctacgtaa
     1321 aagagcatta atctttgcgt tgttatctgt ttttcttgag gatgagcact ttataccgct
     1381 gcttttgaac gttttgcaac cgaacatgcg aacacgagtt tgtacggtta tcaataataa
     1441 tatcgcccat gagtggacac tagcccgaat cgccagcgag ctgttgatga gtccaagcct
     1501 gttaaagaaa aaattgcgcg aagaagagac atcatattca cagttgctta ctgagtgtag
     1561 aatgcaacgt gctttgcaac ttattgttat acatggcttt tcaattaagc gagtcgcagt
     1621 gtcctgtgga tatcacagcg tgtcgtattt catttacgtc tttcgaaatt attatgggat
     1681 gacgcccaca gagtatcagg agcgatcggc gcagggattg ccgaaccgtg actcggcggc
     1741 aagtattgtt gcgcaaggga atttttacgg cactaaccgt tctgcggaag gaataagatt
     1801 atagagtttt actcagacat aaaaaaaacc cggcataggg gaccgggaag aggatagtct
     1861 gccgtctcca gactaataaa ccgttataac actccctgtt ggcacgggaa actttgtgct
     1921 ctcagtaagt taaatataac ttttacagga aataagatca gccatttttt tataaacata
     1981 agctatacgc tgtgcgaaaa tatattcgtg ctgcatttac ttattatcaa ttaactgtta
     2041 tgcaaaacta ctttgtggat aaattttggt cctaccaaat ctggcagttt ttgcgctaag
     2101 aaacagtctg tcatcatttc attagtatac tgaaattgaa ataatcgcag tatgaaatat
     2161 aagggataat catgactcat gtctgctcgg tgatcctcat tcgtcgttca ttcgatattt
     2221 atcatgaaca gcaaaaaata tcgctgcata acgagagtat cctgctgctg gagaaaaatt
     2281 tggcagacga ttttgcgttt tgttcaccgg atacgcgacg actggatatc gatgagctga
     2341 cagtttgcca ttacttacaa aatattcgtc agctaccacg caatttaggg ttacatagca
     2401 aagaccgtct gttaattaac cagtcacccc ccatgccgct ggtgacggcg atttttgata
     2461 gcttcaatga atccggggta aattcaccga tactgagcaa tatgctctac ctttcctgtt
     2521 tatcgatgtt ttctcataag aaagaactga tccccttact tttcaatagc atcagcactg
     2581 tttcaggaaa agttgaacgc cttattagct ttgatatcgc caaacgttgg tatctgcgcg
     2641 atatcgcgga aagaatgtat accagcgaga gtctcatcaa aaaaaagttg caggatgaaa
     2701 atacctgttt cagtaaaata ttactcgctt ccaggatgtc gatggccaga cgattactcg
     2761 agttacgtca aattcctctg catactattg cggaaaaatg tggctatagc agtacgtcgt
     2821 actttataaa cacatttcga caatattatg gtgtaacgcc acatcagttt gcgcaacatt
     2881 cgccaggtac cttttcctga catattttgc atttgaatat tggtcaggat ctcacacctg
     2941 cttcatgtga aactcttccc tgatgatttc tgccgggcta ccggctagtt ctctttcgca
     3001 gtttgccagg cggtataggt atttttagga ggaaaagact cggcatgttt gggattatta
     3061 agctgacaat tcatacgatt acgggaatgt gggtcagtat tgtgttgttc aaattgatga
     3121 cgaatggctg gagtggtttc tactttcagt gctgtgtact aagcctggta ttcctgacgg
     3181 ttagctggtt gttgagtggt gaatggttag caggaaagag taaggctgaa ccttcacgtt
     3241 caaccttact ctcatttaca cgttacgctt ttttaaagcg ggcaaagaga tgttccacta
     3301 caacgaaaaa gaccggaacg aagtaaattg ccagcactgt tgcggcaaac atcccgccca
     3361 tcacgccggt acctaccgcg ttttgcgcgc cagaaccggc accatgactg ataaccagcg
     3421 gcagcacgcc gagaataaag gccagagagg tcatcaggat tgggcgtaaa cgcatccgcg
     3481 ccgcttcgat gattgcctct atcggcgttt tcccttcttt ctgcatcatc tcaacggcaa
     3541 attcgacaat caggatggcg ttcttggcgg aaagcccgat ggtggtcagc aaaccaacct
     3601 ggaagtagac gtcattactt aagccgcgca gatcggtggc cagtaatgcg ccaacgacgc
     3661 ctaacggaac aaccaacatc accgagaacg gaattgacca gctctcatag agtgcggcga
     3721 gggcgaggaa caccacgacc agtgaaatgg catacagtgc aggagcctgg tttgaggata
     3781 acgcttcctg atacgacagt ccggtccatg agtagccgac gcctgccgga agtttagcga
     3841 ccaggtctgc cataaatttc atagcgtcac cggtactttt cccggccgcc gcttcaccta
     3901 aaatctccat tgacgggatg ccgttgtagc gttccaatcg cggtgaacca taggtccatt
     3961 cggtagacga gtaggcagaa agcggtgcca tcgtgccaga ggcgttgcgt acataccatt
     4021 ggttgatgtt atccggcaac atacggaacg gcgtgcctgc ctggacatac acttttttca
     4081 cccgcccctg gttgaggaag tcgttcacgt agctgctgcc gaaggcggtg gaaattgtct
     4141 ggttgatatc agacagcgcc acacccattg cctcagcttt cgcggcgttg acgttcactt
     4201 tgaacatcgg cgtatcttcc aggccgttcg ggcgtacccc ggtgacctgg tcaggcgatt
     4261 gcgctgccag tgataacagc tcgtttcgtg cctaggttag tttttcgtgc cccaggttac
     4321 cgttgtccag cagttccata tcaaaacctg acgcggtacc cagttcagcc accgcgggta
     4381 agttgaacgg gaagacgacg gctttattga tactgcttaa cgcaatcatt gcccgctgaa
     4441 tgatcgcggt aaccgagttt tcctcaccga cacgttcaga ccacggcttg agactgataa
     4501 acgccaggcc gttgttttgc ccctggccgc tgaagccaaa gccgccaacg gtaaacaccg
     4561 actgaacatt atctttctct tttgtaagat aataatccgt cacctgttgc agtactttcg
     4621 tggtgttaac catggtggca ccggaaggta actgcgcggt ggtcataaat accccctgat
     4681 cctcttctgg taagaaagag gtcggtgtac gcaggaacag caccgccatc ccggcgcaaa
     4741 tcagcaggta gaccaccatg tagcgaccgg tacaacgcaa cagcgagcgg gtgctatcgg
     4801 tatagtgctg agttgatttt tcaaacagcg tgttgaagcg tgcgaacagg gcgttaggtt
     4861 tgtgaccgcc ttccggcgtg gctttcagaa tggtggcgca cagggcaggg gtcaggctca
     4921 ttgccacaaa tactgaaagc cgcatggagg agatcagcgt gatggagaac tggcggtaga
     4981 tctccccggt tgcaccgctc ataaaggcca tcggcataaa cactgcagaa agaacaacgg
     5041 caatcccgac cagcgcacgt tggatctgcc ccatcgattt atgcgtcgct tccttcggcg
     5101 gtagcttatc ttccgcaatg acacgctcga cgttctccac caccacgatg gcgtcatcca
     5161 ccagtaaccc tatcgccagc accatcccga acatagtcaa cgtgttgatg gtgaaaccga
     5221 ccgccgacaa gatcgcaaac gtcccgagaa taaccaccgg tacggcaatt gtcgggatga
     5281 tggtggcacg gaaattctgc aaaaacagat aaatgaccag gaagacgagg atgatagcct
     5341 caaccagtgt tttgaaaact tcctgaatag aaatttcgat aaacggcgtg gtgtcgtaag
     5401 gataaaccgt cttcagactt gcggggaaat aggctgataa gcggttcagc tcctctttga
     5461 ctgcccgcga ggtatccagg gcgtttgctc cggcagccag tttgatggcg atcccggcaa
     5521 caggtttgcc gttatagcgc gccacggtgg aataatcttc cgccccaagt tcgacgcggg
     5581 cgacatcacg cagcagcact tgcgaaccat cttgctgaac tttcaacagg attttgccaa
     5641 attcttccgg cgtttgcaga cgcgtctgca caatgatcga ggcgtttagt tgctggtctg
     5701 ccgcctgtgg catgccaccc agttgaccac cagaaatctg gttgttttgc accttaatct
     5761 gggaaataac atcggaaggt accaggttat atttattgag tttttgcggg tccagccaga
     5821 tacgcatggc gtactcggaa ccaaagagct gtacgctacc aacgcccgcg gtacggctta
     5881 gcgggtcttt gatattagac gctacatagt ccgcgatatc gtactggttg aggctgccgt
     5941 tatcagaaat aaacgccgct accatcagga tattactgct cgacttatcg acgctaatcc
     6001 cctgttgctg caccgcttca ggtaatgaag gcatagcgag ttgcagttta ttttgcacct
     6061 gaacctgtgc gatatcagga gatgccccag tctcgaaggt cagagtgata gaggcattac
     6121 ccgccgcatc actggttgaa gacatgtaca tcaggccatc aagcccattc atattttgct
     6181 caatcacctg agtgaccgag tcttctaccg tttgcgcatc ggcacccgga taggtagcgc
     6241 tgacggtaat ggtcggtggc gcaatctgcg gatactgcgc aaccggtaag ttcatgatcg
     6301 ccagaccacc tgcaagcatc ataataatgg caagtaccca ggcaaaaacc gggcgatcaa
     6361 taaaatagtt agccatgaaa gtccccttaa gcctgcaacg ttattgtttc gattcggtgc
     6421 tggcgttttc ctggctggag gaaattgctc gtgctttgat acccggacga atgcgttgca
     6481 aaccggaaac gatcacccga tcgccagcct gcaagccaga ggtgacaacc cactggtcgc
     6541 cgatggcttt gctggcttca atttcgcgta gctgcacgac atcgtcttta tccagaatga
     6601 gcgccgttgc tttaccctgg gcgttgtggg t
//
LOCUS       DAJTRH010000161         6466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTRH010000161 DAJTRH010000000
VERSION     DAJTRH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..2342
                     /locus_tag="OO339_003780"
     CDS             <1..2342
                     /locus_tag="OO339_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5928232.1"
                     /translation="TQIRQEVKLRDGKVIVRGKVAARRTVKSADIVLYHKPGIPLAVI
                     EAKANKHEIGKGMQQGIEYARLLDVPFVFATNGDGFIFRDATAAEGECLEKQITLDDF
                     PSPAELWQKFCLWKGYTQAQLPVITQDYYDDGSGKSPRYYQLQAINKTIEAVSNGQNR
                     VLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDFQPFGTAMTK
                     VSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSASEDSAWR
                     EILDYFSSATQIGLTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKVVRVDI
                     DVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTMVIDERTELVARTITDYLKRTNPM
                     DKTIVFCNDIDHAERMRRALVNLNPQQVKKNDKYVMKITGDDEIGKAQLDNFINPKKP
                     YPVIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTILD
                     FKKATELFADERFDGIPEKVMDTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPPA
                     PPYQVTDTDDVGPFPEEDEKKIRKFHVNGVAVGVIAQRVQYYDADGKLVTESFKDYTR
                     KTLLKEYASLDDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGKDLDPFDMLCHVV
                     YGQPPLTRKERAENVHKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKPF
                     DSMGTLPEIIKTGFGDRNGYNQALSELENEIYQLPPRSA"
     gene            2409..3878
                     /locus_tag="OO339_003781"
     CDS             2409..3878
                     /locus_tag="OO339_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5928233.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            3878..5539
                     /locus_tag="OO339_003782"
     CDS             3878..5539
                     /locus_tag="OO339_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5928234.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5760..6101
                     /gene="symE"
                     /locus_tag="OO339_003783"
     CDS             5760..6101
                     /gene="symE"
                     /locus_tag="OO339_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5928235.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6148..>6466)
                     /locus_tag="OO339_003784"
     CDS             complement(6148..>6466)
                     /locus_tag="OO339_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928236.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1901 a         1543 c         1493 g         1529 t
ORIGIN      
        1 caacgcaaat cagacaggag gtcaaactcc gggacggtaa agtcatcgta cgtggtaaag
       61 ttgcggcacg cagaacggta aaatctgctg acatcgtgct gtaccacaag ccaggcattc
      121 ccttagccgt gattgaagca aaagccaaca aacatgaaat tggcaaaggg atgcaacagg
      181 gcattgaata tgcgcgcctg ctggacgttc cttttgtttt tgccaccaac ggtgatggct
      241 ttatcttccg cgatgccacc gcagccgaag gtgaatgcct tgaaaagcaa ataacgctgg
      301 atgacttccc ctcccccgct gaactctggc aaaaattctg cctctggaaa ggatacacac
      361 aagctcagct tccggtgatt actcaggact attacgacga tggcagcggc aaatcgcctc
      421 gttattatca acttcaggca atcaacaaaa ccattgaagc cgtctccaac gggcaaaacc
      481 gcgttctgct ggtcatggcg accggaacag ggaaaaccta taccgcattc cagatcatct
      541 ggcgcctgtg gaaatcaaaa aataaaaaac gcattttgtt ccttgccgat cgcaacattc
      601 tggtcgacca aaccaaaaat aatgatttcc agccatttgg tacggcaatg accaaagtca
      661 gcggacgcac cattgatccc gcttatgaaa ttcacctcgc gctctatcag gctataactg
      721 gcccggagga agaccaaaaa gcgtttaaac aagtcgcacc agatttcttc gatctgatcg
      781 tgatcgacga atgccatcgc ggcagcgcat ccgaagacag cgcctggcga gagatccttg
      841 attatttcag ttccgccacc caaattggct taaccgccac gccaaaagag acgcatgaag
      901 tctccagcac ggattacttc ggcgatccgg tttacgtcta ctcactaaaa gaagggatcg
      961 aagacggctt cctcgctcct tataaagttg tccgtgttga tattgatgtc gacctgcaag
     1021 gctggcgccc aaccaaaggg caaactgact taaacggcga agtgattgac gatcgtatct
     1081 ataaccagaa agatttcgat cgcacgatgg tgatcgacga acgcacagaa ctggttgcca
     1141 gaaccattac cgactacctc aagcgcacca atccgatgga taaaaccatc gtcttctgta
     1201 acgacatcga tcatgcagaa cgtatgcgcc gcgccctggt taatctcaac ccgcagcagg
     1261 tgaaaaagaa cgacaaatac gtcatgaaaa tcaccggcga tgatgaaatt ggcaaagctc
     1321 agttggataa cttcatcaac ccgaaaaaac cgtacccggt tatcgcgacc acttcagagc
     1381 tgatgaccac tggtgtggat gctaaaacct gcaaactggt agtactagac cagaacatcc
     1441 agtcgatgac caaattcaag caaattatcg gtcgtggtac acgcatcgac gaacgttacg
     1501 gcaaactctg gtttaccatc ctcgacttta aaaaagccac cgaactgttt gccgatgagc
     1561 gtttcgatgg cattcccgaa aaagtcatgg ataccacacc agaggatatc gccgatccag
     1621 aatctgattt tgaagagaaa ctcgaagaaa tcagcgaaca tgacgacgaa caggtaacag
     1681 gcgttgatga accgcctgcg ccaccatacc aggttaccga taccgatgat gtcggcccat
     1741 ttccggaaga agacgagaag aaaatccgca agtttcacgt caacggtgta gcggtgggcg
     1801 ttattgcaca gcgcgttcag tattacgacg ccgacggtaa actggttacc gaatccttta
     1861 aagattacac ccgcaaaaca ctgctcaaag aatatgcctc gctggatgac tttacccgca
     1921 agtggcagga cgccgatcgc aaagaagcga tcattcacga gctggagcaa caggggatca
     1981 tctgggaagt actggcagaa gaagtcggta aagatctcga cccgttcgac atgctttgcc
     2041 acgtagtgta tggtcagccg ccgttaaccc gcaaagagcg cgccgagaac gtgcacaagc
     2101 ggaactactt cacaaaatac tctgaagcag cgcaagccgt gctcgataat ctgctggata
     2161 aatacgccga tgcgggcgta caggagatcg aaagtattca ggtgctgaaa cttaaaccat
     2221 tcgacagcat gggcacctta ccggagatta ttaaaaccgg atttggcgac cgtaacgggt
     2281 ataatcaggc gctcagcgag ctggaaaacg aaatctacca attaccgccc cgctctgctt
     2341 aacaactctc ttttgcgcct tctgcaacca tcatgttgca gaaggccttg tttttcatgg
     2401 aatacaacat gtctatcagc tcagtaatca aatcattaca agatattatg cgcaaagatg
     2461 ccggtgtgga cggcgatgcg cagcgtctcg gtcagctctc ctggctgctg tttttgaaaa
     2521 tcttcgatgc ccaggaagag gcgctggaac tggagcagga taactatcaa tatccgatcc
     2581 cacagcgtta tttgtggcgc agttgggccg ccaacgcgca gggcattacc ggtgattccc
     2641 tgctggaatt cgttaatgat gatctgtttc cggcgttgaa aaacctcact gcgcctatcg
     2701 ataaaaaccc acgcggctac gtggtgaagc aggcgttcag cgatgcctat aactatatga
     2761 aaaacggtac gctactgcgc caggtgatca acaagctgaa cgaaattgac tttaccagcg
     2821 ccagcgaacg ccatctgttt ggtgatattt acgaacagat ccttaaagat ctgcaatctg
     2881 cgggcaatgc gggcgaattc tatactccac gcgccgtcac tcgctttatg gtggatcgcg
     2941 ttgatccgaa actcggcgaa tccattatgg acccggcctg cggtacgggc ggttttcttg
     3001 cctgcgcgtt cgatcatgta aagaacaaat acgtgaagag cgtcgccgat catcagacac
     3061 tgcaacaaca gatccacggt gttgagaaaa aacagcttcc gcacctgctg gcgaccacca
     3121 atatgctgct gcacggcatt gaagtaccgg tacaaattcg tcacgacaac accctgaata
     3181 aaccgctttc ctcctgggat gagcaactgg atgtcattgt taccaacccg ccgtttggtg
     3241 gcacggaaga agacggtatt gagaagaact ttccggcaga gatgcaaacc cgcgaaacgg
     3301 cggatctgtt tctgcaactg attgtggaag tactggcgaa aaacggtcgt gcggcggtgg
     3361 tattgccgga tggcacacta tttggcgaag gtgttaaaac caaaatcaaa aagctactta
     3421 ccgaagagtg caacctgcat accatcgtgc gtttaccgaa tggtgtgttt aatccctata
     3481 ccggcattaa aaccaacctg ttgttcttta ccaaaggtca gccaaccaaa gagatttggt
     3541 tctatgaaca tccgtatccg gcgggcgtga aaaactacag caaaaccaag ccgatgaagt
     3601 ttgaagagtt tcaggcggag atcgactggt ggggtaacga ggccgatggt tttgccagcc
     3661 gcgtcgagaa tgagcaagcg tggaaagtca gcattgatga cgtgattgcg cgcaacttca
     3721 atctggatat taaaaaccca catcaggcgg aaaccgtcag ccatgatccg gacgaactgt
     3781 tagcgcagta tgcaaaacag caggcggaga tccagacgct gcgtaatcaa ctgcgcgata
     3841 ttcttggcgc tgcgctgtct ggcaaggagg ttaactaatg agtgtggaaa agctgatcgt
     3901 tgatcatatg gaaacctgga cctcggcgtt gcaaacccgt tccaccgccg ggcgcggcag
     3961 ctccggtaag attgatttgt atggtattaa gaaattgcgt gagctgattc tggaactggc
     4021 ggtgcgcggt aaactggtgc cgcaggatcc gaatgatgaa ccggcgtcgg acctgctgaa
     4081 gcgtattgcg gcggaaaaag cagagctggt gaagcaaggc aaaattaaaa agcaaaaacc
     4141 actgccagaa attagtgagg aagagaagcc gtttgaattg ccggagggat gggagtgggt
     4201 gcacctccct gatatttatt gctcaatttc tgagtcatct agaaaaataa aatcctccga
     4261 aatactacct gagggaaaat atcctgttat cgagcaaagc caagaattta tttctggtta
     4321 ttgcaacaat gaatgtctgt taataaagtt aaataatcca gttatagttt ttggtgatca
     4381 cacaaggaat attaaattta ttgattttga ttttgttgtc ggcgctgacg gtgtgaagat
     4441 tttatcacct atattaattt gtgagcgttt ctttttttgg cagttacgat catttaaatt
     4501 agatgtaaga ggatacgcga gacattttaa agtattaaat agctgcttat ttgcattacc
     4561 ccccattgca gaacaagagc gaatagttga aaaagttagc tctttgatgt ccctctgcga
     4621 ccaactagaa cagcaatccc tgaccagtct ggacgcacat cagcaactgg ttgaaaccct
     4681 gttgggaacg cttacagaca gccaaaacac cgcggaactg gctgaaaact gggcgcgtat
     4741 tagcgagcat ttcgacaccc tatttaccac cgaagccagc gtggatgcgt taaaacagac
     4801 cattctgcaa ctggccgtaa tgggcaaact tgtgccgcag gatccgaatg acgaacccgc
     4861 ctctgaactg ctcaaacgta ttgcgcagga aaaagctcaa ttggtgaaag aagggaaaat
     4921 aaaaaaacaa aaaccactgc cgccaattag cgatgaggaa aaaccgtttg aacttccgga
     4981 agggtgggaa tggtgtcgta ttggtaatat agttaatatt aaatctgaat tagtatcacc
     5041 aaaagattat ttaaatttat atcaagtagc acctgatatt attgaaaagg gcactgggag
     5101 agtgatatca aagagaacag tgaaagaatc aggagtcaaa gggccaaata gtcgctttta
     5161 taaaggacaa attgtttatt ctaaaatccg cccgtcatta tccaaagttt ttttagcaga
     5221 gtataatggt ctttgcagtg ctgatatgta tccattagac tgttacatta atccaaatta
     5281 cttattgaaa tatatccttt ccattccatt tcttatgcaa gtcaaaaaag cagagaacag
     5341 aataaaaatg ccaaaactta attctgacag tttttacaat attattgttg cgattccccc
     5401 ctataatgaa caacaagcaa tatttgataa gataaattct attgaagcag tatgcaatgg
     5461 tcttatttct tatattggga tttatcataa aacccaactg catcttgcag acgcactcac
     5521 tgacgcagcg ataaactaat tccctcgccc tcacaaaacc aataaagcgg ctaaatagcc
     5581 gctttattca catcaccaaa atttatattt cacgctaatt ttcatcttat tttcattaat
     5641 ttttagctgg actttccctg tatttactga tgatgtatcc aggtatttag cacggtgcgg
     5701 atgtgcgcca acacactcgc accgctaatc acaatcacta tttatagaga atagtaatca
     5761 tgactgacac gcattctatt gcacaaccgt tagaagcaga agtctccccg gcaaataatc
     5821 gtcaattaac cgtgagttat gcgagtcgtt acccggatta cagccgtatt cccgccatca
     5881 ccctgaaagg tcagtggctg gaagccgccg gttttgccac cggcacggcg gtagatgtca
     5941 aagtgatgga aggctgtatt gtcctcaccg cccaacccgc cgccgccgaa gagagcgaac
     6001 tgatgcagtc actacgcaag gtgtgtaagc tgtcggcgcg taaacaaagg caggtacagg
     6061 aatttattgg ggtaattaca ggtaaacaga aagtcgcctg atacattgac ccggtagcgc
     6121 cagtgctacc gggtattatt cgccgcatta taccaacgcg ctctccatct gtttttcaaa
     6181 tgcctgacga attaatccca taatgtaagg aagttgtgcg aggtcgctga aaccaatttc
     6241 tacatcgcca ttaccccagc gcccaacatt tgtcacatct ttggcaatac ctttcggatc
     6301 gaccagttca tgaaactgca tattaagcga caatcgcagt cggctttttt gcggcacaac
     6361 atcaacaaaa ttcgtttccg ctttaaacgc aatatatagc ttcaatactt cctgcgtgat
     6421 ccctgcgtcc aggcgcataa cttcatcgcg aagatgatcg aataac
//
LOCUS       DAJTRH010000162         6315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTRH010000162 DAJTRH010000000
VERSION     DAJTRH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..845
                     /locus_tag="OO339_003785"
     CDS             <1..845
                     /locus_tag="OO339_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5928237.1"
                     /translation="LGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIG
                     IDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNI
                     FYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDIL
                     YFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQ
                     RLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAYKASDERLYVNKQNK
                     NSRS"
     gene            888..1391
                     /gene="yeaK"
                     /locus_tag="OO339_003786"
     CDS             888..1391
                     /gene="yeaK"
                     /locus_tag="OO339_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5928238.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1392..1496)
                     /locus_tag="OO339_003787"
     CDS             complement(1392..1496)
                     /locus_tag="OO339_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928239.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1666..2112
                     /locus_tag="OO339_003788"
     CDS             1666..2112
                     /locus_tag="OO339_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5928240.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2069..2887)
                     /gene="nimR"
                     /locus_tag="OO339_003789"
                     /pseudo
     CDS             complement(2069..2887)
                     /gene="nimR"
                     /locus_tag="OO339_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            2984..4164
                     /gene="nimT"
                     /locus_tag="OO339_003790"
                     /pseudo
     CDS             2984..4164
                     /gene="nimT"
                     /locus_tag="OO339_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4219..4566
                     /locus_tag="OO339_003791"
     CDS             4219..4566
                     /locus_tag="OO339_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5928241.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4588..4842)
                     /gene="yoaF"
                     /locus_tag="OO339_003792"
     CDS             complement(4588..4842)
                     /gene="yoaF"
                     /locus_tag="OO339_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5928242.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5025..6050
                     /gene="dgcP"
                     /locus_tag="OO339_003793"
     CDS             5025..6050
                     /gene="dgcP"
                     /locus_tag="OO339_003793"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5928243.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6083..6181
                     /locus_tag="OO339_003794"
     CDS             6083..6181
                     /locus_tag="OO339_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5928244.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6184..6258
                     /gene="yoaJ"
                     /locus_tag="OO339_003795"
     CDS             6184..6258
                     /gene="yoaJ"
                     /locus_tag="OO339_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5928245.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1521 a         1520 c         1551 g         1723 t
ORIGIN      
        1 gtcttgggat ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct
       61 atttttatga gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt
      121 caggaagtat tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat
      181 tgtcgagcat ttaccaggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg
      241 tatatgtcgc agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatttgc
      301 ggaatatctt ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa
      361 ccgataccga ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt
      421 atgtcagtta cgtccatgac ttacctggcg gcattcgtgt ctcgttatcc attgatattc
      481 tttactttat cacgtcttcg aggaaaagcg ttctgttctg gattttgacg gcgttaattt
      541 tgctgaatat ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta
      601 gtgatgcgat gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt
      661 tgcagaaact ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt
      721 taaagcaaat aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttacatg
      781 atgcgtataa agcttccgat gagcgtttat atgtcaataa gcagaacaaa aacagccgtt
      841 catgataacc ttctgtggtt gtttgcttgt aatctcagga gcgtgaaatg actgaaatgg
      901 ctaaaggaag cgtgacgcat cagcagttaa tcgctttatt atcacaagaa ggtgctaact
      961 tccgcgtcgt tacccatgaa gcggtaggta aatgtgaagc ggtgtcagaa attcgtggta
     1021 ccgctctagg ccagggtgct aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc
     1081 aacatgttct ggcgatcctc gcagccgatc aacaggccga tctgagccaa ctcgccagcc
     1141 atataggcgg attacgcgct tcgttggcca gcccggcgga agttgatgaa ctcacaggct
     1201 gtgtcttcgg cgcgatcccc cctttcagct tccatccaaa actcaaactg gttgccgacc
     1261 ctctactctt cgaacgattt gatgaaatcg ccttcaatgc agggatgctg gataaatccg
     1321 ttattttgaa aaccgcggat tatttgcgca ttgcgcaacc agaactggtc aatttccgcc
     1381 gcactgcgta gctagcctat ccgttcaata agcagaacgg acaaaaccag cacaaccgca
     1441 atagcaaaaa acgatgatgt gataatcagt gtctcgacga acatttgatc gttcatagca
     1501 tgcccccggg agatagctgt tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg
     1561 acagttcgcc tgatttttta tgcaaaaaac gtaaagattt tctacttcct tcctgcagca
     1621 agcataaagt aagcaggctt attatttttt ggcaaggaaa ccacgatgtt tgatgtcact
     1681 ttgctgatcc tgctcgggtt agctgcgctg ggctttatca gtcataacac cactgtcgcc
     1741 gtttcaattc tggtgttgat cattgtccgc gtcacaccgt taagcacctt tttcccgtgg
     1801 attgaaaaac aggggctaag tatcgggata atcattctga cgattggcgt tatggccccc
     1861 atcgccagcg ggacgctacc gccctcaacc ttgattcact cgtttctgaa ctggaaatcg
     1921 ctggtggcaa ttgcagtagg cgttattgtc tcctggctgg gtgggcgcgg cgtgacgtta
     1981 atgggcagcc agccgcaact ggttgctggg ctgctggtgg gcactgtttt aggcgtagcg
     2041 ctgtttcgcg gcgtaccggt tggcccgctt attgccgcgg gtctggtttc gctgattgtg
     2101 gggaaacagt agttaatccc gcaatatatc ggcctggcgt ttgccccaag cctttcttaa
     2161 acatagtaat aaaggccgtt gttgaatcat accccagcgt atgagcaact ttctgtacag
     2221 tatcgccttt cactaatccc tgaagcgcca taatcagctg taactgctga cgccactggc
     2281 gaaaactcaa ccccgtctct ttcactatca ggcgggcaag attgcgttca ctcatcgcaa
     2341 aaaagccagc ccattgcccc aatgcccccc actcgacggg ccctttcgcc atcatctcca
     2401 ccatcgtgcg gattttagga tgagaagaaa cgggcaggtg cagctgttgt tgtggctgct
     2461 gagggagttc atcaaacagg acctgaatta atctgcgggt catcggctca gcacgctgag
     2521 tcgtcgtcct gtttgccagg gttaaaatca attcccggca taagggcgat attttcaatg
     2581 tgcaacatgg tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg
     2641 ttagctgtca cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc
     2701 ggcaccatcc acaaagcatt ttccaccgta caggtaattg caccatgtaa tgccagtatc
     2761 agctgtcctt tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata
     2821 caaaatgcga ccgcggcttc atggtgtcgg tgggttcata gccattgaga ttcaacctgt
     2881 gcatcatttt gtccgaactt agcgataatt tgtcatttta gcttgattca acataacaat
     2941 aaaaacggta aggtacagcc tcgtttgtaa caatgagaag catatgacct gttcaacttc
     3001 attaagcggc aaaaacagga ttgtccttat cgctggcatt ctgatgattg ccacaacatt
     3061 acgcgtcacc tttaccggcg cagcgccgtt actggatacg attcgttccg cttactcgct
     3121 gacgacagcg caaaccggct tattaaccac cctgccatta ttggcctttg cgctaatctc
     3181 acctctggct gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt
     3241 acttttgatc tgtgcaggta tcgcaattcg ttctctccct tcgccttact tattatttgg
     3301 cggtacagcg gtcattggcg gtgggattgc attaggcaat gtcttactgc caggattaat
     3361 taaacgcgat ttccctcatt ccgtcgccag acttaccggc gcatattccc tgacaatggg
     3421 agctgcagcg gcactgggat cggctatggt cgtgccgctg gctttgaacg gttttggctg
     3481 gcaaggcgcg ttgttcatgc tgatgtgttt tcctctgctg gctctttttt tatggctgcc
     3541 acagtggcga agtcagcaac atgcaaattt gagtacctca cgcgccttac atactcgggg
     3601 tatctggcgt tcgccacttg cctggcaggt aacattgttt cttgggatca actcactggt
     3661 ctattacgtg attattggct ggcttccggc gatcctcatc agtcacggct atagcgaagc
     3721 acaggcgggt tcactgcatg gtttgctgca actagccaca gcagctcccg gtttgctgat
     3781 cgcacttttc ttacatcatg tgaaagacca gcgtggtatt gcagcgttcg ttgccttgat
     3841 gtgcgcagtg ggcgcggttg ggctctgctt tatgccagcg cacgcgatca cctggactct
     3901 gcttttcggt tttggttccg gcgcaacaat gatactgggg ttgacgttca ttggtcttcg
     3961 gcaagttctg cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg
     4021 ctggcagcct gtgggccgcc gctaatgggt aaaatacacg atgctaacgg taactggtcc
     4081 gtaccactga tgggtgttgc aatactttca ctactgatgg cgattttcgg actttgcgcc
     4141 gggagagaca aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcttgcgtg
     4201 aaagggagaa caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag
     4261 agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat
     4321 ttagcccttg atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt
     4381 cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat atcgcgcaga actggcgcaa
     4441 cacgagcaag aaggaaagcg gctggcggac atcgccaaaa aacagccgct gaccctgctc
     4501 tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc tggccgactg gctacgtagc
     4561 ttgtgatttt agtacggcat ccggcggtta tttttcacca gccggatggt cccgtcgcca
     4621 caatgcccat tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg
     4681 cgctgtctgc actggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt
     4741 tgccatacca atctgcggcg gtttaggtgc ttctggctga gaaggggttg aacaaccagc
     4801 caggaccagc aagcaaggca gaacaaaact gataattttc atttattgat ctcacatatt
     4861 tatccaagct tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc
     4921 actcaaaaca tattacgtct tgtttcatct ttgttgatga tgttttatca tgcctgcaaa
     4981 gattaaataa tcagcactta cccgccgtat cctggagttg ttccgtgtca gatcagatta
     5041 tcgcccgcgt ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg gtccgacagc
     5101 ttctggagat gctggaaatg gtcactgata tggaatcaac ctacctgacc aaagtggatg
     5161 tcgaagcgcg cctgcagcat attatgtttg cccgtaacag ccagaaaatg cacatcccgg
     5221 agaattttac cgtcttgtgg gattactcgt tatgcaaacg cgccattgat gaaaactgct
     5281 ttttcagcga tgaagtccct gaccgttggg gcgactgtat tgcggcacgc aatcttggca
     5341 tcaccacatt tctgagcacg ccaattcact taccggatgg atcattctat ggcacgcttt
     5401 gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt ttacagttat
     5461 tcgccggact gattgcacaa tatattcaaa aagaggcact ggttgaacag ctgcacgaag
     5521 ccaatgctgc gctgattgcg caatcgtata ccgactcgtt aaccgggcta ccgaatcggc
     5581 gggcgatttt tgaaaatctg acgacgctgt tttccctcgc ccggcatctt aaccataaga
     5641 taatgatcgc gtttatcgat ctggataact tcaaattaat caatgatcgt tttggtcata
     5701 atagtggcga tctgtttctc attcaggttg gcgagcgcct taatacgctc cagcaaaatg
     5761 gcgaagttat tggtcgtctc ggtggtgatg agtttttggt tgtttcacta aacaacgaga
     5821 atgcggatat ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga gaatatcact
     5881 taggtgatgt tgatttgtat tatcccggtg ccagtcttgg catagtagaa gtcgatcctg
     5941 aaacaaccga tgcagacagt gccctgcatg ctgccgatat tgcgatgtat caggagaaaa
     6001 aacacaaaca gaaaacacct tttgtcgcgc atccagcgct acattcctga ggcgtattca
     6061 catccttttg attggtgata acatgcgaat cggtattatt tttccggttg taatcttcat
     6121 tacagcggtc gtatttttag catggttttt tattggctgc tatgctgccc cgggagcata
     6181 aagatgaaaa aaacaacgat tattatgatg ggtgtggcga ttattgtcgt actcggcact
     6241 gagctgggat ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg tgtgcagcct
     6301 ttgtgcaatt taagc
//
LOCUS       DAJTRH010000163         6200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTRH010000163 DAJTRH010000000
VERSION     DAJTRH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO339_003796"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO339_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5928246.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO339_003797"
     CDS             2089..2238
                     /locus_tag="OO339_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928247.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO339_003798"
     CDS             complement(2983..3423)
                     /locus_tag="OO339_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5928248.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO339_003799"
     CDS             3641..4030
                     /locus_tag="OO339_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928249.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO339_003800"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO339_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OO339_003801"
     CDS             complement(4585..5421)
                     /locus_tag="OO339_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5928250.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="OO339_003802"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OO339_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5928251.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1431 a         1618 c         1641 g         1510 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aacaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgttgcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJTRH010000164         6052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTRH010000164 DAJTRH010000000
VERSION     DAJTRH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            430..1338
                     /locus_tag="OO339_003803"
     CDS             430..1338
                     /locus_tag="OO339_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5928252.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO339_003804"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO339_003804"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5928253.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="OO339_003805"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="OO339_003805"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5928254.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO339_003806"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO339_003806"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5928255.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="OO339_003807"
     CDS             complement(5673..>6052)
                     /locus_tag="OO339_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928256.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAJTRH010000165         5872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTRH010000165 DAJTRH010000000
VERSION     DAJTRH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            193..465
                     /locus_tag="OO339_003808"
     CDS             193..465
                     /locus_tag="OO339_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5928257.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(431..838)
                     /gene="nudG"
                     /locus_tag="OO339_003809"
     CDS             complement(431..838)
                     /gene="nudG"
                     /locus_tag="OO339_003809"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5928258.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            925..1545
                     /locus_tag="OO339_003810"
     CDS             925..1545
                     /locus_tag="OO339_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5928259.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1554..2861)
                     /gene="ynjE"
                     /locus_tag="OO339_003811"
                     /pseudo
     CDS             complement(1554..2861)
                     /gene="ynjE"
                     /locus_tag="OO339_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(2928..3581)
                     /locus_tag="OO339_003812"
     CDS             complement(2928..3581)
                     /locus_tag="OO339_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5928260.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3581..5052)
                     /locus_tag="OO339_003813"
                     /pseudo
     CDS             complement(3581..5052)
                     /locus_tag="OO339_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5025..5872)
                     /locus_tag="OO339_003814"
                     /pseudo
     CDS             complement(5025..5872)
                     /locus_tag="OO339_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1378 a         1591 c         1553 g         1350 t
ORIGIN      
        1 tttatgtcat gtgcttttgc agttttcgac tataccatca agaatgtgac ggtaagcaag
       61 aaaaaaatga cccaggaaag caaattaata acgagagtaa tctcataacc aaatgcgata
      121 actcagacgc taaagttttg ccacccggct accgttatca gtagcagaga caataacgtt
      181 aaggagttcc ctgtgagtcg agcattgttc gccgtagtat tagcatttcc attaattgca
      241 ctggctaacc cgcattatcg tccggacgtg gaggtaaacg taccgccaga agtgttcagt
      301 tcaggcgggc agtcagcgca gccctgtact cagtgctgcg tttatcagga tcaaaattat
      361 tctgaagggg cggtaattaa agccgaagga atactgctgc aatgccagcg tgatgacaaa
      421 acccttagca ctaatccgct gctctggcgg cgcgtaaagc cataaacgcc tctaataatg
      481 gaatgtcagc aggggccagc ggatattgca gcgcctcttc aggtgagcac cagaccagcg
      541 cctgatgttc atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag gcatgaagat
      601 ggataatccg gcccgaaact tctcgctgat ggctggcaac atactcaccc acagttgctt
      661 cgatgtccaa ttcttcgttt aactcacgca ccagcgcctg ctgctggctt tcatccagct
      721 cgactttacc tccggcaaac tcccataatc ccgcctgatc gctctgggcg gggcgttgcg
      781 cgagtaaaat tttgccatca cgttcaatga tggcggcaac aacttcaatc attttcataa
      841 attacacacg ggcagataag cgaaaaatgc cataatatcc gcactgttgc tcaatcagaa
      901 aggctttata tttaaggagt gcctgtgcta gaccgccatc ttcatccccg gattaaaccg
      961 ttgttgcatc agtgcgtgcg ggttcttgat aaaccgggca ttacgccaga tggtttaaca
     1021 ttagtgggat ttgccatcgg cgtgctggct ctgccgtttc tggcgctggg ctggtatctg
     1081 gcggcgctag tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg tgcgctggcg
     1141 cggcgcagag ggcttaccga cgcgggcggc tttcttgata tttctctcga ttttctcttt
     1201 tacgcgctgg tgccgtttgg ctttattctt gctgcaccgg agcaaaatgc gctggcaggc
     1261 ggctggttgt tgtttgcgtt tatcggcacg ggcagcagtt ttctcgcctt tgctgcactg
     1321 gcagcaaaac atcagattga taaccccggt tatgcacata agtcgtttta ctatctgggg
     1381 ggattaaccg aaggcacaga aacgatctta ctgtttgtgc tgggatgttt attccctgca
     1441 tggtttgcgt ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac ctttacgcgg
     1501 gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc agtaattgca atgttatttg
     1561 ctactgcccg ggccgcgttc accggttgca accggatttt ttggatcgct gctccattcg
     1621 taccagccgc cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc gtacataaag
     1681 gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc tggtttgata
     1741 ttccatgctt tccacatagc ggtaatatca tcggcgctac gcatggtgcc atccgggtta
     1801 tagaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt
     1861 tcaccttttg gtttaatata gctgtaaccg ctggtcgtac cgataaattc tggccacgaa
     1921 cgaatgctca ccagcgatgc atcctggcga tgcagcagtc cacgggcctg ttccatatcg
     1981 agcatcaact gcggttgtgc cgggatcttc acgccgaaat ccggttccgc tttcactttc
     2041 ggtggcgttc ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca gccgccatcc
     2101 agcagacgca catctttcac gcctgcgtag agcataattt gcgccacacg cgctccagcg
     2161 tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc
     2221 atcgctttta gttgttcatc agaaactttg ttccacagcg gttcgctttc cacttcgttg
     2281 gtatcgatgt agtcagcgcc aggaatatgg ctgataaggt aaagcttagg agcgccctag
     2341 gccgcttcaa tgactttcca gtcaccgaca ggtttcgccg taacctcttt accttgttgc
     2401 aggtcatgca gccattgcgg ataaaccagc tgctcaaagt gcggcagttt ttgtagacgg
     2461 gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg taaaacctgc tttttgcagt
     2521 cgcgttttga cggcgtcaac atctttgcca ttaccgtaca gcgccaccgg ggcatcggct
     2581 ttcaggttat gttgcttgat ccacgcgttg agctgttcgg tgctcatttt gtccagccag
     2641 ctagcagaga ggtttaaggc ggcaggttca tgaccagaag ggccatttaa ggtttgtggc
     2701 cagccgttat aaaacgcgct ggggcgagta tctatcgctt tgccattttg ttgctgaagt
     2761 tggtcaagtg taagaggctt cgccagttca gcggcccacg aagaagcgca agccagccct
     2821 aaagccattg ccagcgcggt catttgagaa acacgtttca tcgaaaatcc cgacattgta
     2881 gttttgagtg cgccattctg aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg
     2941 ttgtaatttt ctgaccactg cgccatatcc agaacagaac tatcagcagg aacatcctgg
     3001 agatcgtgcg ttacctgaac gacggggatc gccagggcgc gaacttcgct gaacacccac
     3061 tggcgaaaat tatcgcgcag agccacatca agacggctga atggctcatc caggagtaac
     3121 gcttttggct gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg accgccagac
     3181 aaagtggcag gatcctgatg gaaagctccg tcaaggcctg aacgctcaag tgcatcattc
     3241 acggcattac gtcgggcatt ccccttaagt gtcgccggta gcgccagcag taaattttgc
     3301 ccgacgctga actggtcaaa taacagtgca tcctgaaaaa gaatgccaat ctgacgctgt
     3361 gcggtgggta gcatgtcaat ccgttgctca ttgagccata gctcacctgt acaagaaaac
     3421 tgtccggcca gtgcaccaat catccatgaa aacagagtgg attttccaca gccagacggc
     3481 cccattaacg tgacgatgtc acctttatca accgtaaagt taacgtttgt cagcaagcgg
     3541 ctttctggta aacgtagcga aacatttttc acgcagagca ttagcggatt ccttgtctga
     3601 cataacctac ccattttgcg actaacgcgg tcagggcaaa aataataagc ggtaacaaca
     3661 gttgccataa cgcctgggcg gcgagaatac cgttgctgcc gccgctgctt aatgccaccg
     3721 cctcagtggt gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc atatactgcg
     3781 caatactgac tgaaaatccc accgcgaagg caatcagcac agggcgcaac atgagtgggc
     3841 atttcacgta aaagaagatt ttggcccgcg accagcccag tgtttgcgca atcaaaatta
     3901 accgtgaatc aatgcgctgc caggcgggtt gcaggataaa cagcatccac ggcattaccc
     3961 acagcagatg cccccagacc accgccgtcc agcttccatc caggttcagc catagcgcca
     4021 gcgtgtactg gcccgccacc agtggcagag cgggcaatag aatgggcaac catagccaca
     4081 actggcgacg ctgtgccccc cattccagcc acagcagtaa caggagcaaa gcgatgaatg
     4141 ttgctaccag ccccattgtc agactgttga tgagtgcttc actattgatc gtcgactgat
     4201 ccgcgaggat cgccagcaga accacacaca gcacaccggt taagggtaaa aaactcgcca
     4261 gcgtagtgcc tggtaataaa ggtgtggcag gcttgcgaat gccatctacg cggggaatag
     4321 tgcgccgcca gctgcgccaa aacgtaggcg gcgagtaaca gcatcagcag cagactggcg
     4381 agtgcgcctt tcgtttgttg atccgcgtcg ccctgagtta accactgcca gctaattact
     4441 gccagcgtcg gtggattacc tggcccaaga ataatcgcca catccacgac cgaaagcgac
     4501 caggcaacaa tcgccagcat cgccattgcc agcggctgta gcccagcgaa tccagcacaa
     4561 tgacctgctg caacagccgt ttttcgctca atactgccac taagatccac agcagaaatg
     4621 cgctttcttt cactgcaagg gtgaggccca gaccgatcca aatcggtcca ttggcggagt
     4681 gaaatacggg aaatagtcat atagcagccc tccgtcagca aagagcagca gggcgctggt
     4741 ggcaaaagcc acatggggaa tggcgagcag ccacggcaga cgggcgcaca tacgctgcca
     4801 tttcggccca ggccatagcg ccacaatcac cagcagggca atcaacaatg ccccgaccgc
     4861 cgcgatggtt gtcgacacca gcgttgccag taatgcctgc ggtaactgtg gatcggcaaa
     4921 taacgcctgc cagcgtgtca acgacaacgc aggcgatatc agcgtgagag ctgccgggat
     4981 cagcggtgca taaatcaccg ccaccatcgc ccacagcaaa aagattaatg cgtaccgtaa
     5041 cggcgtaacc attcttgttc cagtgcattc acccatcctg cgtgcggttc agccagtacc
     5101 ggcggcagat cctgcggcat tcttgattgc aatatttcgc gctgcccgtc aggcagtttt
     5161 tgcggatcga gaacagaagg atcgccccag acaacgggat ctgctttacg cagttgcgca
     5221 tcgggtgaga gcaggaaatt ggcaactacc ttcgccgcag cactggcatt cgcgttggct
     5281 ggaatggtga cgaaatgcac gttgccaatc atcccctcgc gaaagccaaa actgtaactg
     5341 cttgccggca aatcgccgct ggcgattttt tgctgcgcat gtgcggggtt aaaggtcagc
     5401 gacaggcgca atgtgccggc tttcagcaga gcatccatcc gcgcgggcga aggcgggaaa
     5461 tcctttcctt cgcgccacaa atacggatgc agtgcatcaa gatattgcta caagggcgca
     5521 gtgacacggg cgaaagtcgc atcgtccggc gcttctttta atgcggcggg atctggcgtc
     5581 agcataatca gcaactgttc aagaaacgcc gtgccggtaa agtccggtgg gcgcggataa
     5641 gtaaccgtgc cgggattggc tttagcaaac tccagtaagg cttgcggcgt ttgtggtggc
     5701 tgtggcgtaa catcgcggcg ggcgataaac gtcagttgtg cgccgcccca gggcgattcc
     5761 gcaccttgtg tcggcactga aaaatctccc cgcaccggca gctgtgtgtc gacatagcgc
     5821 cagttgggca gagtctccgc ccagcccgtt tgtaataaat tggcctcttt ta
//
LOCUS       DAJTRH010000166         5810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTRH010000166 DAJTRH010000000
VERSION     DAJTRH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            65..583
                     /gene="mddA"
                     /locus_tag="OO339_003815"
     CDS             65..583
                     /gene="mddA"
                     /locus_tag="OO339_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5928261.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            580..1030
                     /locus_tag="OO339_003816"
                     /pseudo
     CDS             580..1030
                     /locus_tag="OO339_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(1031..1264)
                     /locus_tag="OO339_003817"
     CDS             complement(1031..1264)
                     /locus_tag="OO339_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5928262.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1350..1523)
                     /gene="ortT"
                     /locus_tag="OO339_003818"
     CDS             complement(1350..1523)
                     /gene="ortT"
                     /locus_tag="OO339_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5928263.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1718..1813
                     /gene="yncL"
                     /locus_tag="OO339_003819"
     CDS             1718..1813
                     /gene="yncL"
                     /locus_tag="OO339_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5928264.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2215..3024)
                     /locus_tag="OO339_003820"
     CDS             complement(2215..3024)
                     /locus_tag="OO339_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5928265.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3234..4658)
                     /gene="patD"
                     /locus_tag="OO339_003821"
     CDS             complement(3234..4658)
                     /gene="patD"
                     /locus_tag="OO339_003821"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5928266.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4680..5474)
                     /locus_tag="OO339_003822"
     CDS             complement(4680..5474)
                     /locus_tag="OO339_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5928267.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5464..>5810)
                     /locus_tag="OO339_003823"
     CDS             complement(5464..>5810)
                     /locus_tag="OO339_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5928268.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1523 a         1476 c         1288 g         1523 t
ORIGIN      
        1 gttgcttcgg gctgttttcg atagtattga taacatcatc atcattattt tattgaggcc
       61 agacatgtcc atccgttttg cccgcaaagc cgactgtgct gccattgcgg aaatttataa
      121 ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa acggtggatg cagataaccg
      181 cattgcctgg tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga
      241 tggcgtagtt acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca
      301 taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa
      361 attgttaagt cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg
      421 gatcgaatcg caaaatcagg cttcgctgca tctgcaccag tcgctgggat ttgtcgtcac
      481 cgcacaaatg ccgcaggtag gcactaaatt tggtcattgg ctggatctga catttatgca
      541 gttgcaactc gacgagcgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt
      601 gcttttttga ttgccgccgg aattggtctg gtagtcccag aacacgctga tggcgcgcat
      661 cacccagacc tcctctacca ttctcatcgc catgttgctg aactcactgg tggggattgt
      721 gctctttgtt tctattttat ggtttaaaca aggcatggcg gggtttggcg aactggtgtc
      781 cagcgtgcgc tggtggacac ttatacctgg cttgctgggt tcgttttttg tcttcgccag
      841 tatcagcgga tatcagaatg tgggagcggc gacaactatt gcggtactgg tcgccagcca
      901 gctgattggc ggtctggtgc tggatatctt ccgcagtcat ggcgtaccac tacgggcgtt
      961 gttcggccca atatgtggcg caattttact tgtggttggc gcctggttgg tggccagacg
     1021 ctcattttaa tcagaggatg gtgccgcctt tggttaactg ttcgtggcgc gcttccatat
     1081 cttctttatg cttgcgaccg tgatgagcaa tagcggtgcg caaacgctgc tgctgggtgt
     1141 agcgatcttc ccgacttaac tccgcgtcat cgctcagggc gattaataat tcgttcatat
     1201 gggcaatgac gctctcttca atcgcggcat ccacgcgcgc gatgacttca tccagatgcg
     1261 acatactttc tccttagcca cttgtgaaat ttatcgatta atccaaatga tataagtctg
     1321 cgtggtgccg tcaagccagt gagtaagatt taaaacagtg aaaacaacag cgccaccggg
     1381 aaactcatcg gccatgtggc ccccaccaga aaggcgctta agaaacggat gcgtttctta
     1441 tcgtgagaaa gaaaccaggt gataagaaat gcgattgctg ccataaccgc ataaaaaaca
     1501 agcatatgtt gatagagaaa catgtacggg cgtccagcca tatgaataaa acgcgcgcaa
     1561 atatgcgcca ttttttgacg tgcatcaagt tttttcacca gtttgagaaa aaatcatcaa
     1621 acagcaactt gcccttatcc atcttcagaa tgagtattat tgcggatttt cttaacccgt
     1681 actatacaca taggggcaat gataaaaggt ggcaaaaatg aatgtttcca gtagaactgt
     1741 agtactgata aatgtctttg ctgctgttgg tttgtttact cttatctcta tgagatttgg
     1801 ctggtttatt tgatgtagtg attttgcacg cagcacgtac tgtcagagaa atcaactctc
     1861 tgacagtacg caagatcttc tcatactctc caagtttgca acattatcct taatatttac
     1921 ttctttttgc tggcatctac attgaaatga gtagaaaaca tccccctact gtcgtctata
     1981 caaacagctt actgattcaa acggaatctt gttttcacat ttttctatca cgaatacttt
     2041 atacgcgcgg gagtctataa atgctttata ctttcaagga ataacgtaaa tataaaaaga
     2101 tggcgtaaag tatatgcatg gcaggatgtt tgcaacctcc ttgatgacgc tgccccattg
     2161 aaattgcaaa ccccgccctg gttatcttgc ccatttatca tgcaaaacag ataattatag
     2221 gcctctgaat acctcagaaa gattgaattt tttatagtcc ctctcttgaa tatttccatt
     2281 tgatatcaat cgattcatct cttcaacaaa ataatttata tcatcacact tattattatc
     2341 cggaagatct gagtagttat aattccacca ttttattctc aacagttcat caataacatt
     2401 tgaatcaaac ctaaatctga ttatttttgc aggaactcct gcaactatgg catatggggg
     2461 cacatctttg gtaacaacgg aattggcagc aataacagca ccatcaccta ttgcgatgcc
     2521 acctttcaat acaacatcat ttcctatcca gacatcattt cctataatcg gattttcagg
     2581 tttggtaatg aatggtttca atgtccagct acgttcaaat tcactcatag caaacttatc
     2641 aaattcaccg ttataagtca tcatgtgagt agtaaaacga ttcaaaggat gatttccacc
     2701 cattattttt acatttgatg caatactaca aaatcgacct atacgtatat tccctgaaaa
     2761 atggcaggtc ggaaatgaaa aagcgccgac actggagaaa tttttcacag gcattgttga
     2821 atattcttca gcaataacat tatcttcaat tgttatcttg tcaccttttg agaaaagaaa
     2881 taaattcctt tctttatacg acttcttccc cttatgattt aaaaagattt tattatccat
     2941 acaaaactca aagtgttttt tcgtccatct gaaggtaagg gttttatttt tatcttccgt
     3001 gaatttgtac aattgaactt tcattgttaa tacttctact tataaagtat tagtttaata
     3061 acgatactct aaagccttat gctaatcaac tgtgacagga tgaaaaatcg ccatcaaaca
     3121 tttagcctaa tttttacgcc agttattaat gtaatcattc taatacttaa ccgccattaa
     3181 actcagaacc taaaacgata gtggccgtcc tataacagcc actaacatct aacttaatgt
     3241 ttaaccatga cgtggcggac gacggtgtaa tcctccagcc cataaagtga catatccttg
     3301 ccgtaaccag aaagtttctg cccaccgtgc ggcatttcac ttaccagcat gaaatgggta
     3361 ttgacccagg tacaaccata ttgcagccgt gcgctgacgc gatgcgccct gcccacatct
     3421 ttcgtccata ccgaagatgc aagtccgtac tggctgtcat tcgcccagtt caccacctgt
     3481 tcttcgttgt cgaagggcgt aacactcact actggaccaa atacctcttt ttgcacgatg
     3541 gcatcgtcct gtaatgcgcc agccagcagc gtcggcgcat agtaatagcc attacccttg
     3601 cgcttttcac cgccagtgat cactttgatg tgccctgtcg ctttcgcctc ttctactgcc
     3661 ttgctgacgc gttcgagatg cgccagcgag cttaaaggtc caagctccgt agactcgtca
     3721 tccggcgcac cagattttaa cgttgccacc gcagcaccca gtttttccac cagcgtatcg
     3781 taaatgcctt tttgcgcgta gatccgacaa gccgcagtac aatcctgtcc agcattgtaa
     3841 tagccaaatg tacgtacacc ttcgaccact gcttcaatat ccgcatcatc aaaaacaatc
     3901 actggcgctt tgccaccaag ttccatatga gtacgcttaa tggacgacgc ggtatggctg
     3961 atgatgtgct cgccggtggc gatagagccc gtcagcgaca ccatccgcac tttgggatga
     4021 ccggtcagcg gatcacccac cgttttgcct ctgccaaaca gtatgttaat cacgcctgcc
     4081 gggaagatat ctttcgccag ctctgccaac ttcaacgcgg tcagcggggt aatttctgat
     4141 ggtttaagca ctacgcagtt ccctgccgcc agcgccggag caagtttcca cgcggccatc
     4201 atcagcggat aattccacgg tgcgatagaa gccacgaccc ccaacggatc gcgacggatc
     4261 atcgaagtat gaccttcaag atattcacct gccgccagac cattcagaca gcgcgccgca
     4321 cccgcgaaaa agcgaaaaac atcgacaatc gccgggattt catcattgaa cgcactatgc
     4381 agcggtttgc cacaattacg ggattccagt tcggcaaaaa cctgaccatt ttcttcgata
     4441 acatccgcca gtttcagcag gcattccgca cgcgctttcg gtgtggtttg cccccattca
     4501 gcaaatgctg catctgccgc acgcacagca gcatcgacct gctctgcgga tacctcggca
     4561 atctccagta aaacgtcccc cgttgccgga ttatagacag gctgtttttc cccttcgccg
     4621 ctaacccgtt ctccgttaat cagtaactta tgttgcatag cttattcctg tatcagtggt
     4681 tattgaccat tgtcgccttc gcgggttagc caccaggccc ccaggatcgg caaggttgtt
     4741 accaacataa ccagcagtgc caccacgttg gttaccggta catcacgcgg tcgcccaagc
     4801 tgattgagca accacaacgg taacgttcgt tcatgacctg ccgtaaaggt cgtaacgatg
     4861 atttcatcga acgacaaggc aaacgccagc attcctcctg ccagtaacgc cgaactgaga
     4921 ttcggcaaca ctacgtagcg gaaggtttgc cagccattgg ccccaagatc cattgacgcc
     4981 tcaaccagac tccacgaggt gcggcgaaaa cgggcgatga cattgttaaa caccaccact
     5041 acgcaaaaag tcgcatgacc gaccacgatg gtgaaaaatc ccggctccag attgatggtt
     5101 ttaaaggcgg ttaatagcgc cagaccagtg acaatgcccg gcagcgcaat gggcagcagc
     5161 aataacagcg aaatggcgtt tttgccgaaa aagtctcgtc tccacagcgc ggcagctgcc
     5221 agcgtcccta acaccagcgc aattaatgtc gccagcgccg ccactttaag tgacagcgtc
     5281 acggcatcaa gaatatcact acgctgtgct gccacgctaa accagcgcag cgtcaggccc
     5341 tgcggtggaa aactaaacgc cgcatcttca gtgttaaacg cataggcggc gattatcagg
     5401 atgggaaaat gtaggaaaac aacgccgccc caggccgcca gtttgaggaa aaacggtgcg
     5461 cgttcagagt gcatcgaacg ctcccagacg tttcacgaac gccaggtaca gtgcgatgag
     5521 aataatcggc accagggtga atgccgccgc catcggcata ttgccaatcg ccccctgctg
     5581 ggaataaacc atattgccga taaaatatcc tggaggacca accagctgcg ggacgataaa
     5641 atcgcccagt gtgagtgaga aggtaaagat agagccagcg gcaatacccg ggattgccag
     5701 cggcagcacc acatagcgaa aggtttgtcg tggacgtgcg ccgagatcag ccgacgcctg
     5761 caacaatgac ggcggcaaac gctcaagcgc cgcctgaacg ggcaggatca
//
LOCUS       DAJTRH010000167         5764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTRH010000167 DAJTRH010000000
VERSION     DAJTRH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(1..393)
                     /locus_tag="OO339_003824"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO339_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO339_003825"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO339_003825"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5928269.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO339_003826"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO339_003826"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5928270.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1781 a         1308 c          954 g         1721 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctgg
//
LOCUS       DAJTRH010000168         5749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTRH010000168 DAJTRH010000000
VERSION     DAJTRH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            17..355
                     /gene="spa13"
                     /locus_tag="OO339_003827"
                     /gene_synonym="spaM"
     CDS             17..355
                     /gene="spa13"
                     /locus_tag="OO339_003827"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5928271.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            342..1220
                     /gene="spa32"
                     /locus_tag="OO339_003828"
     CDS             342..1220
                     /gene="spa32"
                     /locus_tag="OO339_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5928272.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            1214..2095
                     /gene="spa33"
                     /locus_tag="OO339_003829"
                     /gene_synonym="spaO"
     CDS             1214..2095
                     /gene="spa33"
                     /locus_tag="OO339_003829"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5928273.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            2100..2750
                     /gene="spa24"
                     /locus_tag="OO339_003830"
                     /gene_synonym="spaP"
     CDS             2100..2750
                     /gene="spa24"
                     /locus_tag="OO339_003830"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5928274.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            2765..3025
                     /gene="spaQ"
                     /locus_tag="OO339_003831"
     CDS             2765..3025
                     /gene="spaQ"
                     /locus_tag="OO339_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5928275.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            3025..3795
                     /gene="spa29"
                     /locus_tag="OO339_003832"
                     /gene_synonym="spaR"
     CDS             3025..3795
                     /gene="spa29"
                     /locus_tag="OO339_003832"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5928276.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            3802..4830
                     /gene="spa40"
                     /locus_tag="OO339_003833"
                     /gene_synonym="spaS"
     CDS             3802..4830
                     /gene="spa40"
                     /locus_tag="OO339_003833"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5928277.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            4843..5085
                     /locus_tag="OO339_003834"
     CDS             4843..5085
                     /locus_tag="OO339_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928278.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         1824 a          827 c         1060 g         2038 t
ORIGIN      
        1 ctctttgtat cgagatgtgg aggcattaga taaaaggatt atatacttcc tgcaactgga
       61 gaatgactta gaacctgtag gcgctcagag tgttagtcag cttttcaata caagacgtaa
      121 gatcgctata gtaaaaaagc atattatcca atatcagtct gaacgaattc ttctaaaggg
      181 aagaattgaa gaaattcaaa aagatattga tgaggcaaat gcttctaaaa gaaaactgct
      241 tcataaagag agtaaaatat gtaaacggat tggcttgatt aaaagaaata actttgccaa
      301 acagttgata cttgatgagt tatcacaaga ggatatgaag tatggcatta gataatataa
      361 acctaaattt tagtagtgac aaacagatag aaaaatgtga gaaactatct tcaatagata
      421 atattgatag tctcgttttg aagaagaaga ggaaggttga aattcctgag tactctttaa
      481 ttgcatctaa ctattttact attgataagc actttgaaca taagcatgat aaaggagaaa
      541 tttatagtgg cattaaaaat gcgttcgaac ttagaaacga acgagcgaca tattctgata
      601 ttccagaatc aatggccatt aaagaaaata ttttgatacc agatcaagat atcaaagcaa
      661 gggaaaaaat aaatatcggc gatatgaggg ggatcttttc atataataag agtggaaatg
      721 cagacaagaa cttcgaaaga agtcatactt cttctgtaaa ccctgataat ctgctagaat
      781 ctgataatag aaatggtcaa attggtttaa aaaatcatag cttgtctatt gataagaata
      841 ttgctgacat catttcttta ctaaatggaa gtgttgctaa atcatttgag ctgcctgtaa
      901 tgaataaaaa tactgcagac ataaccccat ccatgtcatt gcaagaaaaa tcaatagttg
      961 aaaatgataa aaatgttttt caaaaaaata gtgaaatgac ttaccacttt aaacagtggg
     1021 gggctggaca ttctgttagt atttcagtgg agtctggttc ttttgttcta aaaccgtcag
     1081 atcaatttgt aggaaataaa cttgacttaa ttttgaaaca agatgctgag ggtaattaca
     1141 gatttgatag cagtcaacat aataagggga ataaaaataa tagtacagga tataatgaac
     1201 agagtgaaga agaatgctaa gaattaaaca ttttgacgct aacgaaaaac tacagatttt
     1261 atatgcaaag caactctgcg agcgtttttc gattcagaca ttcaaaaata aatttacagg
     1321 cagtgaaagt ttagtcactc ttacttccgt gtgtggggat tgggtaattc gtattgatac
     1381 attatctttt ttgaaaaaaa aatacgaggt attttcagga ttttctacac aagaatcttt
     1441 actgcattta tcaaaatgtg tctttataga gtcgtcatct gtattttcga ttccagaact
     1501 gtctgataag attactttcc ggatcacgaa tgaaatccag tatgcaacta ctgggagtca
     1561 tttatgctgt ttttcctctt ctttaggtat tatttatttt gacaagatgc cggtattacg
     1621 taatcaagtt tctcttgact cattgcatca tcttttagag ttttgcttag gttcatctaa
     1681 tgtaaggctg gctactttaa aaagaattcg cactggtgat ataatcatag ttcagaaact
     1741 ttataattta ttattgtgta atcaagttat tattggggat tatattgtga atgataataa
     1801 tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca gaacacacag aagtttcttt
     1861 ggcattattc aattatgatg atatcaatgt aaaagtggac tttattcttt tagaaaaaaa
     1921 tatgacaatc aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt ttcccgatga
     1981 catagttaaa catgtaaata ttaaagtaaa tggttctttg gttgggcatg gggaacttgt
     2041 ttctattgag gatggttatg gtatcgagat tagttcttgg atggtaaagg agtaatctca
     2101 tgctgagtga catgtccctc atcgctacac tttctttttt tacgctatta ccatttttag
     2161 ttgctgctgg tacctgttat ataaagtttt ctattgtttt tgtaatggta cgaaatgcac
     2221 ttggtttgca gcaggttcca tcaaatatga ctctcaatgg tattgcattg attatggctt
     2281 tgttcgtgat gaaacccatt attgaagctg gttatgaaaa ttatctaaat ggccctcaaa
     2341 aatttgatac aatatctgat attgtccgtt tttctgattc tggcttgatg gaatataagc
     2401 aatacttgaa aaagcataca gatcttgagt tagcacgttt tttccaaagg tctgaagaag
     2461 aaaatgctga tttaaagagt gcagagaata atgattattc attgttttcc ctcttgcctg
     2521 catatgcgct aagtgaaatc aaagatgcgt tcaaaatagg tttttattta tatttgccat
     2581 ttgttgttgt tgatcttgtt atttcaagca ttttacttgc acttggtatg atgatgatga
     2641 gtccaatcac aatatcagta ccaataaaac ttgtattatt tgttgcgctt gatgggtggg
     2701 gtatcctgtc taaggccttg attgaacaat atatcaatat tcctgcttag atgatggagg
     2761 taatgtgtct gatatagttt atatgggtaa taaggctctt tatttaatcc ttatcttttc
     2821 cttatggcca gtaggtatag ctacggttat tggattatct attggtttgt tacagacagt
     2881 gactcaactc caagagcaga cacttccttt tggtataaag cttataggtg tctcaatatc
     2941 tttgctactt ctttctggat ggtatggtga ggttttattg tctttttgtc atgaaataat
     3001 gtttttaatt aagagtgggg tttgatggac atttcaagct ggttcgaaag tattcatgtg
     3061 tttttaatac tcctgaacgg cgtttttttt agattggctc cattattttt ctttcttcca
     3121 tttttaaata acggtataat ttctccatct attagaatac ctgtgatttt tcttgttgca
     3181 tcaggattaa ttacttctgg taaggtagac ataggttctt ctgtttttga acatgtttat
     3241 ttccttatgt tcaaggaaat aattgttggc ctccttctct ctttttgctt gtctcttccc
     3301 ttttggatat ttcatgctgt tggtagcatt attgacaacc agcgtggggc aacgcttagt
     3361 agttcaattg atcctgccaa tggtgttgat acgtctgagt tggcaaaatt tttcaatctt
     3421 ttttctgcag ttgtatttct atacagtggt ggtatggtct ttattttaga atccatacaa
     3481 ttgtcttata atatatgccc gttattttct caatgttctt tccgtgtctc aaatatctta
     3541 acatttctga ctttattggc aagtcaggct gttattttag ccagtcctgt tatgatagta
     3601 ttgttactat cagaagtatt acttggtgta ttatcgagat ttgctccgca gatgaatgct
     3661 ttttccgtat cattaactat taaaagttta cttgcaatat ttattatttt catctgttct
     3721 tctactattt acttttctaa agttcaattt ttcctcggtg aacataaatt tttcacaaat
     3781 ctatttgtta gataaaatat tatggcaaat aaaacagaaa agccgacacc taaaaaacta
     3841 aaggatgccg caaaaaaagg acagtcattt aaatttaagg atttaacgac tgttgttatt
     3901 attctggtag ggacatttac tataatatca ttcttttcct taagtgatgt aatgctctta
     3961 tacagatatg taataattaa tgacttcgaa attaatgagg gtaaatactt ttttgcagtg
     4021 gttattgtct tttttaagat aattggcttc ccactttttt tctgtgttct ttcggctgtg
     4081 ttgccaacat tggttcaaac aaagtttgtt cttgcgacta aagctatcaa gattgatttt
     4141 tcagtattaa accctgttaa agggttaaaa aaaatattta gtataaagac aataaaagaa
     4201 tttttcaaaa gcattctgct tcttattatt ctagcattaa caacctattt cttttggatt
     4261 aatgaccgaa aaataatttt ttctcaggtg ttttctagtg ttgatggctt atatcttatt
     4321 tgggggaggc tgtttaagga tataatatta tttttcttgg cattttctat tcttgttatt
     4381 attcttgact ttgtgattga gttcatttta tacatgaaag atatgatgat ggataaacag
     4441 gagataaaaa gagaatatat agagcaagag ggacactttg agacaaagtc gagaaggcgt
     4501 gagttgcata tcgagattct ttcagagcag actaaatctg atatacgtaa ttcaaaatta
     4561 gtggtaatga acccgactca tattgcaatt ggtatttatt ttaatccaga aatagcgcct
     4621 gcacctttta tttctctcat tgaaactaac cagtgtgcct tggctgtcag aaaatatgca
     4681 aatgaagttg gtataccgac tgtgcgtgat gtgaaattag ctagaaaact atataaaaca
     4741 catacaaaat atagttttgt tgattttgaa cacttggatg aagtcctacg tcttattgtt
     4801 tggcttgagc aggttgaaaa cactcattaa taagtaaagg agatgtgtta tatgggagtt
     4861 aatttctgta ataaaatagg tattgatcag tctgaatttg aaatagaatc ttccatcatt
     4921 aactccattg ctaatgaggt attgaaccca atatctttcc ttagcaataa ggatataata
     4981 aatgttttac tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga catttatcgt
     5041 tgtgctctgg agttggtcgt tgaaaaaaca cctgatgatt tataatcatt cataataact
     5101 cttttgctga tataaatatt tttgttgaag atatttatgt agtcacagga tcaattgtag
     5161 aaaattgtta tgtttttgtt aggaactgca ctaaataata tatgataaga cagcaaaaac
     5221 gactgaccat aatccttctt ttattaggag ttgataagag agactattca tcctgtaatg
     5281 ttaaaactct gttatatagt attcgcgatt atgcaaagtc tgtaaatgat catgagatac
     5341 taaccgagag taatcgttta ttatctcatt gcatttcaga tagtaacgga gctttcttta
     5401 aaagtagtaa atacgttccc cttaagtact tacgtaaaag gagaatagca cgaaagatac
     5461 caaatgatta gcactttgct agtaagcttg ctggtaaact atattgaccg ttgggtttgt
     5521 tttgagactg taatttaaat tgtgtaattg cctgtttttg atatgtttac tccaacaacg
     5581 gagacaggta aattatggac gaaaagaagc ttaaagccct tgcggctgaa ctggctaaag
     5641 gcctcaaaac tgaggccgac ctcaatcagt tttcccgtat gctgacgaaa tatcccggct
     5701 cttaaaacga tagtggctca ggctttcatt tttcagcgtt ccccagaag
//
LOCUS       DAJTRH010000169         5738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTRH010000169 DAJTRH010000000
VERSION     DAJTRH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..241
                     /locus_tag="OO339_003835"
     CDS             <1..241
                     /locus_tag="OO339_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928279.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="OO339_003836"
     CDS             complement(238..1179)
                     /locus_tag="OO339_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5928280.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="OO339_003837"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="OO339_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="OO339_003838"
     CDS             complement(1684..2904)
                     /locus_tag="OO339_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5928281.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="OO339_003839"
     CDS             complement(2908..3651)
                     /locus_tag="OO339_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5928282.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="OO339_003840"
     CDS             complement(3542..5008)
                     /locus_tag="OO339_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5928283.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="OO339_003841"
     CDS             complement(5008..5241)
                     /locus_tag="OO339_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5928284.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="OO339_003842"
     CDS             complement(5229..5708)
                     /locus_tag="OO339_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928285.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAJTRH010000170         5691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTRH010000170 DAJTRH010000000
VERSION     DAJTRH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(233..955)
                     /locus_tag="OO339_003843"
                     /pseudo
     CDS             complement(233..955)
                     /locus_tag="OO339_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
     gene            complement(945..1352)
                     /locus_tag="OO339_003844"
                     /pseudo
     CDS             complement(945..1352)
                     /locus_tag="OO339_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416029.1"
                     /note="regulates the transcription of lsr genes; involved
                     in autoinducer 2 uptake, biofilm formation and stress
                     responses; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
     gene            1601..3136
                     /gene="lsrA"
                     /locus_tag="OO339_003845"
     CDS             1601..3136
                     /gene="lsrA"
                     /locus_tag="OO339_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5928286.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3130..4158
                     /gene="lsrC"
                     /locus_tag="OO339_003846"
     CDS             3130..4158
                     /gene="lsrC"
                     /locus_tag="OO339_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5928287.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4158..5150
                     /gene="lsrD"
                     /locus_tag="OO339_003847"
     CDS             4158..5150
                     /gene="lsrD"
                     /locus_tag="OO339_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5928288.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5162..5691
                     /locus_tag="OO339_003848"
                     /pseudo
     CDS             5162..5691
                     /locus_tag="OO339_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
BASE COUNT         1278 a         1439 c         1520 g         1454 t
ORIGIN      
        1 ctatttctcc atgcacaggc caattatgaa agaggagagg cttaagttct tgaaccctga
       61 acgaataacg actaccagaa aagcacttcg catcactcag acatgattta acataatata
      121 cattatgcgt agtaatattg tagtagctaa attgagatgt ccgtctctga ctaaatagac
      181 atgtccgcgc tacagtctca ccacagcgat cactgctttc aaaggatggg actcatgacg
      241 gcatacggaa cggagtaatc ggcaactgat gccgggcctc gctgagcgtg cactgcttat
      301 tatccgtgaa cgtgccgcca agaaccgcgg tttgtgccgg acgcacaacg cctaacccaa
      361 ggcaaccaag ctgcacgtcg cctcctccaa cgaccaccag agtgcccgcc ttcagaccgc
      421 agagttccgc cgcttgtgaa cttaccacgc ccagcaatgt gccggtttct ttgacaggag
      481 aaagaatatc ggcacgcagg ccagccatat ccagcaatgc aggtttccag gcacgggtgg
      541 ttagatcaag aagtcccgtg gtgccagcgt tagagggatc caccgccagt tcgccgctga
      601 gcatataggc cagccagtcg ctgatcatgg tgatggttga tgcctgacgg taaatatcgg
      661 aacgatggtg cgccagccaa agtaatctgg ggatggcact taaagccagt gtttgtccgg
      721 tcgctcgata aacttcgttt tcaaaggtat tgttgtgcag ttctttaagt tcgctaactt
      781 cgcgtgccgc tctggcatcc acattggcgc aggcccagat cggtgttcct tcattgttat
      841 ataaaacaat gccttcacgc atagaacatg ccgaaacggc agcgatatac tccgggtcta
      901 tgccggcgtt gtgcagcgcc tggcgcatac actcacacgc cagttgccag tttttgcagc
      961 gtattcatgg ttgcctcgcc aaaaccaatc gccagcatct gttgtggttg aagtaaactc
     1021 atcaacatat gcgccgcgcc tatccctagt cgcccgccga catcagcatc cgcaagccca
     1081 gggatcaccc ggacatgttg cagcgaaaac tgacgacgta attgagtttc atattccaga
     1141 cagccttcaa agcgagaatt aatctgtacg cgaataatgc cggactgatg ccctttctcc
     1201 agcaagcgcg acactttcaa acgggtcagg ccgagacgat cgctgatctc gctctgggtc
     1261 agcccgtcgt gatagtaaaa ccacgcgatc cgcgcgacct gttcttcttc acacattccc
     1321 tgttctgaaa ttgccgaatc gttgattgtc ataattcatt cttcactttg aacatattta
     1381 aatctttaat gcaattgttc agttcttgcg catttatatc tgtgatggca accacagttt
     1441 gactctacga gcatgaacaa acgcaaccgt gaaaatcaaa atagcataaa ttgtgatcta
     1501 ttcgtcggaa atatgtgcaa tgtccaccta aggttatgaa caaattaaaa gtagaaatac
     1561 atttgttcaa aactcacctg caaaactgaa cgggggaaat atgcaaacga gtgatacccg
     1621 cgcgttaccg ctactttgcg cccgctcggt ttataaacag tattcagggg tcaatgtcct
     1681 gaaaggcatc gattttacgt tgcatcaggg ggaggtccac gccctgctcg gcggcaatgg
     1741 tgccggtaaa tcgacgttaa tgaagattat tgccggtatt acccctgctg atagcggtac
     1801 gctggagatt gggggcaaca actacgccag attaacgcca gttcatgctc atcagctggg
     1861 catttatctc gttccccagg aaccgctgct tttcccaagc ctgtcgataa aagaaaacat
     1921 cctgtttggg ctggcaaaaa aacagctctc catgcagaaa atgaagaact tgctggcggc
     1981 gctgggctgc cagtttgatc tgcatagtct ggcaggatcg ctggatgtcg ccgatcgcca
     2041 aatggtggaa atcctccgcg ggctgatgcg cgactcgcgg attctgatcc tcgatgaacc
     2101 taccgcctcg cttacccctg cggaaaccga acgcttgttt agtcgcttgc aagagctgct
     2161 tgctactggc gtgggtattg tttttatctc gcataagctg ccggaaattc gccagattgc
     2221 cgatcgaatt agcgtgatgc gcgacggaac catcgcctta agcggcaaaa ccagcgaact
     2281 gtctaccgac gacattattc aggccatcac cccagcggta cgggaaaaat cgctctctgc
     2341 cagccaaaaa ttatggctgg agttacctgg taaccgccca caacatgccg tcggaacgcc
     2401 ggtgctgaca ctggaaaatc tgactggcga aggcttcagg aatgtcagcc tgacgctcaa
     2461 tgccggagaa attctgggcc tggctgggct ggtgggagcc ggacgcacag aactggccga
     2521 gacgctctat ggtctgcgta ctttgcgtgg cggacgcatt atgctgaatg gtaaagagat
     2581 caataaatta tccactggag aacgtttact gcgcggtctg gtttatctgc cggaagatcg
     2641 ccagtcatcc ggactgaatc tcgatgcttc gctggcctgg aacgtctgcg cccttactca
     2701 taaccttcgt ggattctggg cgaaaaccgc gaaagataat gccaccctgg aacgttatcg
     2761 tcgggcgctg aatattaaat ttaaccaacc ggaacaagct gcacggacat tatccggtag
     2821 caaccagcaa aaaatcctca ttgccaaatg cctggaagct tcgccgcaag cattgattgt
     2881 cgatgagccg acgcgcggcg tggatgtctc ggcccgtaat gatatctacc agctgttgcg
     2941 cagcatcgcc gcacagaatg tggctgtgct gtttatctcc tctgatatgg aagagatcga
     3001 actgatggca gatcgtgtgt atgtgatgca tcagggcgaa attacccact ctgcactgac
     3061 cgggcgcgat attaatgtcg agactattat gcgcgttgcc ttcggcgata gtcagcgtca
     3121 ggaggcatca tgctgaagtt tattcagaac aaccgtgaaa tcacggcatt gctggcggtg
     3181 gtgctgctgt ttgtattacc cggttttctc gaccgccagt atttaagtgt gcaaacgctg
     3241 accatggttt atagcagcgc gcaaatcctg atcctgctgg caatgggcgc gacgctggta
     3301 atgcttacgc gcaatattga tgtttcagtg ggttcgatta ccggaatgtg cgcggtgctg
     3361 ttggggatgt tactgaacgc aggatattca ctacctgttg cctgtgtcgc gactttactg
     3421 cttggtttgc tcgcgggatt tttcaacggt gccctggtcg cgtggctaaa gatccctgcc
     3481 attgttgcca cccttggcac gttagggttg tacagaggca tcatgttgct gtggactggc
     3541 ggtaaatgga ttgaagggtt acccgccgaa ctgaaacagc tctccgcccc gctgctgctt
     3601 ggcatttcag caattggttg gttgacgata attctggtgg catttatggc ctggctgctg
     3661 gcgaagacgg cgtttggacg cagtttttat gccacgggcg ataatttaca gggcgctcgt
     3721 caactgggcg ttcgtacgga agccattcgc attgtggcat tttcgttgaa cggctgcatg
     3781 gcggcactgg cgggaattgt gtttgcttcg cagattggtt ttatcctcaa ccagaccggt
     3841 accgggctgg agatgaaagc aattgcagcc tgcgtgctgg gcggcattag tttgctcggt
     3901 ggttccggtg cgatcatcgg tgcggtactc ggcgcatggt tcctgacgca gatcgatagc
     3961 gtactggtgc tgttgcgcat tccggcatgg tggaatgatt ttatcgcggg tctggttctg
     4021 ctggcggtgc tggtgtttga tggacgcctg cgttgtgcgc tggaacgtaa tctacggcgg
     4081 caaaaatatg cccgctttat gacgccaccg ccatccgtta aacccgcttc gtcaggtaaa
     4141 aaacgggagg ccgcataatg cgtattcgct acggttggga actggctctt gccgcactgc
     4201 tcgttattga gattgtcgca tttggtgcaa ttaacccgcg aatgttagat ctcaatatgt
     4261 tgctgttcag caccagtgac tttatctgca ttggcattgt cgctctaccg ctgacgatgg
     4321 tgattgtcag tggcgggatc gatatttcgt ttggttcgac catcggcctc tgcgccattg
     4381 cattgggcgt actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac
     4441 tgctcggcgc attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc
     4501 cgctggtgat tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg
     4561 gtatggccgg agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt
     4621 tcgctaacct ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc
     4681 tcgttttctg gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc
     4741 aaagcccgcg cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct
     4801 atgccatgac ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt
     4861 cagcacgttc cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg
     4921 gcggggccaa tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat
     4981 tagtgggata tttgcagcaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg
     5041 ccctttccgg tgcgctgctt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc
     5101 agcaaattaa agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt
     5161 catgacactt catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat
     5221 ctctatgaat gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg
     5281 attttttacc agcggtggca acggcgcaca acaagcgggt aaagagctga gcgttgatgt
     5341 gacctacgac gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt
     5401 cgtcaatcaa ggttataacg ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc
     5461 cggcactgaa acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta
     5521 aaccggagtg ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt
     5581 tggtggatat ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact
     5641 caagccccac cgttacggac caaaaccagt gggtgaaaga agcgaaagcg a
//
LOCUS       DAJTRH010000171         5666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTRH010000171 DAJTRH010000000
VERSION     DAJTRH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..146
                     /locus_tag="OO339_003849"
     CDS             <1..146
                     /locus_tag="OO339_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5928289.1"
                     /translation="LEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKRYRKKLTE
                     FNQ"
     gene            251..1603
                     /gene="celF"
                     /locus_tag="OO339_003850"
                     /gene_synonym="chbF"
     CDS             251..1603
                     /gene="celF"
                     /locus_tag="OO339_003850"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5928290.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1616..2374
                     /gene="chbG"
                     /locus_tag="OO339_003851"
     CDS             1616..2374
                     /gene="chbG"
                     /locus_tag="OO339_003851"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5928291.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2421..4682)
                     /gene="katE"
                     /locus_tag="OO339_003852"
     CDS             complement(2421..4682)
                     /gene="katE"
                     /locus_tag="OO339_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5928292.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4865..5128
                     /gene="cedA"
                     /locus_tag="OO339_003853"
     CDS             4865..5128
                     /gene="cedA"
                     /locus_tag="OO339_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5928293.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5405..>5666
                     /locus_tag="OO339_003854"
     CDS             5405..>5666
                     /locus_tag="OO339_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928294.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQ"
BASE COUNT         1414 a         1242 c         1466 g         1544 t
ORIGIN      
        1 aactggaaat gaccaactat tccgtgacgg atattgcgtt tgaggccggt tatagtagcc
       61 cgagtttgtt tattaaaacg tttaagaaat taacgtcctt tacgcctaag agatatcgta
      121 agaaattgac tgaatttaat cagtaagtcg ttatacctga caattcacat atctgtcctg
      181 ttgctggatt atttatgtcc gggggggcag atatgccaga tatcagtatt ctgtactgaa
      241 gggagaaatt atgagccaga aattaaaagt cgtcactatt ggtggcggga gcagctatac
      301 cccggagtta ctggaaggat ttattaagcg ttatcacgaa ttgccggtca gcgaattatg
      361 gctggtggat gtcgagggtg gcaaagctaa actggatatt attttcgatc tctgccaacg
      421 gatgattgat aacgctggcg tcccgatgaa gctttataaa acgctggatt gccgcgaagc
      481 attgaaagat gctgatttcg ttactaccca actgcgcgtt ggccaattac cggcgcgtga
      541 actggatgaa cgtattccat taagtcatgg ttatcttggt caggaaacca acggcgcggg
      601 cggtttgttt aaaggtctgc gtaccattcc ggtgattttt gacatcgtaa aagatgtcga
      661 agaactttgt ccgaatgcat gggtgattaa cttcactaac ccggcgggaa tggttactga
      721 agccgtttat cgtcataccg gatttaaacg ctttatcggc gtgtgtaata ttccgatcgg
      781 catgaagatg tttattcacg atgttctgat gctgaaagac agcgatgatt tatctatcga
      841 tttgtttggc ctcaaccata tggtgttcat taaggatgtg ctggtaaatg gcaagtcgcg
      901 ctttgccgaa ttgcttgatg gtgtggcatc cgggcagtta aaagtatctg gcgttaaaaa
      961 tattttcgat ctgccattta gcgaaggctt aattcgttct ctgaatctgt tgccgtgttc
     1021 ttatttgctt tattacttca agcaaaaaga gatgctggct attgaaatgg gcgaatacta
     1081 caaaggcggc gcacgagcgc aggtagtaca gaaagtcgag aaacaacttt ttgagctgta
     1141 taaaaacccg gagttgaaag ttaagccgaa agaactggaa cagcgcggtg gggcttatta
     1201 ctctgatgca gcgtgcgaag tgatcaacgc tatctacaac gacaagcaag ctgaacatta
     1261 cgttaatatc ccgcatcatg ggcatattga taatattccg gcagactggg cggtagaaat
     1321 gacctgtaag ctggggcgcg atggcgcgac gccacatccg cgcattacgc atttcgatga
     1381 taaagtgatg gggctgattc acaccattaa aggcttcgag attgctgcca gcaacgccgc
     1441 acttagcgga gaatttaacg atgtgttact ggcgctaaac cttagtccgt tggtgcattc
     1501 cgatcgcgat gctgagctgc tggcacgcga gatgattctg gcgcacgaga aatggctgcc
     1561 aaattttgcc gactgcatcg cagagcttaa aaaagcacat taaccgaggc tgattatgga
     1621 acgcttactg attgttaatg ccgatgattt tggcttaagc aaaggtcaga actacggcat
     1681 tatcgaggcc tgtcgcaatg ggattgtcac atcgacgacg gcgctggtga atgggcaggc
     1741 tattgaccat gcggtgcagt tgagtcgtga tgaaccgagt ctggccatag ggatgcactt
     1801 tgtccttact atgggcaagc cactgacagc tatgccgggg ttaacccgcg atggtgtgct
     1861 gggaaaatgg atctggcagt tggcagaaga agatgcttta ccgctggaag aaattactca
     1921 ggagcttgcc agtcagtatt tgcgtttcat tgagctattt ggacgcaaac ctacgcatct
     1981 tgatagccat catcatgtgc atatgttccc gcagattttc ccgattgttg ccaggtttgc
     2041 ggctgaagag gggattgcgt tgcgcatcga ccgtcagccg ctatctaacg ctggtgattt
     2101 accggctaat ctgcgcagtt cgcagggatt cagtagcgcg ttttatggtg aagagattag
     2161 cgaggcactg ttcctgcaag tgctggatga cgcaagccat cggggtgatc cttcgctgga
     2221 ggtgatgtgt catccggcgt ttatcgataa tactatccgt cagagtgctt actgtttccc
     2281 acggttaacg gagctggaag tgctgacatc agcgtcgttg aaatatgcaa ttgctgagcg
     2341 tggttatcgg ttgggtagtt atcttgatgt gtaagtaagg tggtaaggtc actccccagg
     2401 ggacgtgacc ttatccatat ttacgccgga attttgtcaa tcttaggaat gcgtgaccac
     2461 acgcggtgtt ctgccatcag cgttaacagt tcatccataa aactaccgtc ggtgctgtca
     2521 gcttccacaa tcccttcttc accctggtca gctaccttga ttgttgcttt aaacttgcgc
     2581 gcgtctcccg ccagcgcaat cggtttaagg tgtttgtagg cttccatcag atagtagttg
     2641 gcatcgccgt tgtcagcgat atccgcgata ttgccgcaag ggacaatgac cgcatcgacc
     2701 gtcagcgaag gtgcaccggc aaaggtagcg gctataggca acaccgtacc gtcatccgca
     2761 gtcacttcac ccattcggga gtagagcagt ttggcatgaa cgcctttggc cttcatggcc
     2821 ttgagaatgg ccagaaggtc tgccgatctc acttcatcat taagtaaaat cgctaccacg
     2881 cgacctttca catcaccgtc aggaatggcg tacaaactta aggatggatc ctttttcaga
     2941 ccgttgacgt ccggaggtgg ggtgatattc agctggtcgt cagtcagttc gataccgaga
     3001 tttttcgcca ccgcctgggc cagagtgaga tcaatatgcg ccagctggtc aacaacgcgc
     3061 tcacgaatat acggacgaac gactttgctt aactcaaaac tgaaaccatc gacaatatgg
     3121 cgctgctcga atggcgtctg acttagccag aacagacgcg gatgggaata atattcgcca
     3181 aacgatgggc tgcgctcgcg aactttattg ccttccacgc gctcttggta tgattcaaaa
     3241 ccgccgcgtt tcggccccgg tggtgtttcg cgcggccagt tatcgttgat cgagttcggt
     3301 tcataattcg ccgggttagt gtcgatcccc atacgatgca tgccgtcacg ctggaaatta
     3361 tggtaagggc aggtcgggcg gttaatcgga atctcatgga aattcggccc accaagacga
     3421 ctgatttgtg tatcggtata ggagaacaaa cgtccctgca acaggggatc gttggtgaag
     3481 tccagaccag gaacaatatg acctggatgg aatgctgctt gttcgttttc ggcaaagaag
     3541 ttatccggat tgcgattgag caccattttg ccgacacgct gaacgggcac cagttcttcc
     3601 gggatcagtt tggttggatc gagaagatcg aagtcgaact tgaattcatc ttcttcagga
     3661 atcaactgga agcccagttc gtattccgga aaatcgcctg cttcaatggc ttcccacaac
     3721 tcgcggcggt ggaagtccgg gtcacgtccg gtgagttttt gtgcttcatc ccaaacgagt
     3781 gaggctttac ctgccagtgg tttccagtgg aaacgtacaa acgttgcctt cccttcggca
     3841 ttaatcaggc ggaaggtgtg aataccgaag ccttccatgg tgcggtaact gcgggggatg
     3901 ccgcgatccg acatcgccca catcacgttg tgcagagttt caggttgcag agaaacataa
     3961 tcccagaaag tatcgtgggc actttgccct tgtggaattg cccagtgcgg ttctggtttt
     4021 accgcatgaa caaaatcggg gaatttatgc gcatcctgga taaagaagat tggcgtgtta
     4081 ttgccaacga ggtcaaaaat accctcttca gtatagaact tggtggcaaa accacggata
     4141 tcacgcacgg tatcggcaga gccagcgcca ccctgaacgg tagagaacct aacaaatact
     4201 ggggtgattt tgttcggatc tgagaggaaa tccgctttgg tgatatcgct taagctttta
     4261 tatggctgga aataaccgtg agctgctgag ccgcgtgcat gaacaatacg ttccgggatg
     4321 cgctcatggt caaagtgggt gattttctcg cgcagaataa aatcttccag cagcgttgga
     4381 ccacggttac cggcacgcag tgagttttga tcatcggcga tgcgcacgcc ctgattagtg
     4441 gtcagcgcat aattttcact gcctttgcgt acgtcttcca gagaattaag tttttcgtta
     4501 cgtgtatcag gggctttcag gctcccaggg gcggtaggtt gtgcacctgg cggtgttggt
     4561 tcagccgctg gacgatgaga tccgtcctca ggtgccagtg agtccatccc cggtttcgct
     4621 tcgctggaat cgtgtagtgg tgactggtgc tgatgtgggt tcttttcgtt atgttgcgac
     4681 attgaactcg tctccttaat ttattactga aagggccgct gttttggtca aaaaactctc
     4741 taactataga ccaccagcca gatcccgctt cggagactat tctggaaacg gtgttatgaa
     4801 atacacgcaa agcgggacgt acaggggcta aatcggctaa actacagttt ttctaatttt
     4861 taatatgcgt ttagtgaagc cagtaatgaa gaaaccgctc cgccagcaaa accgccagat
     4921 tattagctat gtcccacgca cggaacccgc gccgccagaa catgcgataa agatggattc
     4981 gtttcgtgat gtctggatgc tgcgtggcaa atatgttgcg tttgtactga tgggagagtc
     5041 atttctgcgc tcaccggcgt ttactgtgcc tgaatctgcc caacgttggg caaatcagat
     5101 ccgccaggaa ggggaagtga ctgagtaagg attacatacc tcccggtttt gccgggagtt
     5161 acaggacctg aaattctgca tttcgttgct acattcattc aaaccacctg taacaaaaag
     5221 ctgcctttga cgagctattg ctataaataa gagatctttt ttctttaatt atttatttat
     5281 tttaaaatgg ttaagttttg ctaatcatga tgagttattt ttgatagttc atcctgtggc
     5341 gtcgctctta aacttatatt gtaaatgact tagatttaaa taagataata attttgttaa
     5401 caatatgagg cgggagatgt tttctacaaa cttaatacag agtaattatg gtgatttaaa
     5461 tattaaaagc ctggctttcg actcttttaa agaaaggctt gagtctacca tggcggcact
     5521 cactttcttt atatcaacgg gtcaatgtga ctgcgatgaa atagcagagt cgaacttcaa
     5581 tcgtatgatt gcctatatgt ctaacattaa ctatgatgcc tcaaagctag gagcgccagc
     5641 acttagtttt gatacgtatc tgcagg
//
LOCUS       DAJTRH010000172         5600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTRH010000172 DAJTRH010000000
VERSION     DAJTRH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..410
                     /locus_tag="OO339_003855"
     CDS             <1..410
                     /locus_tag="OO339_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5928295.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="OO339_003856"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="OO339_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO339_003857"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO339_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5928296.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1331 g         1489 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tctacagcgc aggagagctg
      721 ttgcaggaac tacgtaaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag acctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggcata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggac ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gaccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg ttataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc ggcgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaggaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tactcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatcg
//
LOCUS       DAJTRH010000173         5592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTRH010000173 DAJTRH010000000
VERSION     DAJTRH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(1..279)
                     /locus_tag="OO339_003858"
     CDS             complement(1..279)
                     /locus_tag="OO339_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928297.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO339_003859"
     CDS             220..405
                     /locus_tag="OO339_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928298.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO339_003860"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO339_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5928299.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO339_003861"
     CDS             complement(1254..1670)
                     /locus_tag="OO339_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928300.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO339_003862"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO339_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OO339_003863"
     CDS             1837..2862
                     /locus_tag="OO339_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5928301.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OO339_003864"
     tRNA            complement(2915..3004)
                     /locus_tag="OO339_003864"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OO339_003865"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OO339_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5928302.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OO339_003866"
     tRNA            3996..4071
                     /locus_tag="OO339_003866"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="OO339_003867"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="OO339_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1509 a         1326 c         1293 g         1464 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct at
//
LOCUS       DAJTRH010000174         5441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTRH010000174 DAJTRH010000000
VERSION     DAJTRH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(149..613)
                     /locus_tag="OO339_003868"
     CDS             complement(149..613)
                     /locus_tag="OO339_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5928303.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(610..1485)
                     /gene="nanK"
                     /locus_tag="OO339_003869"
     CDS             complement(610..1485)
                     /gene="nanK"
                     /locus_tag="OO339_003869"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5928304.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1482..2171)
                     /locus_tag="OO339_003870"
     CDS             complement(1482..2171)
                     /locus_tag="OO339_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5928305.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2219..3709)
                     /gene="nanT"
                     /locus_tag="OO339_003871"
     CDS             complement(2219..3709)
                     /gene="nanT"
                     /locus_tag="OO339_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5928306.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(3819..4712)
                     /gene="nanA"
                     /locus_tag="OO339_003872"
     CDS             complement(3819..4712)
                     /gene="nanA"
                     /locus_tag="OO339_003872"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5928307.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(4834..>5441)
                     /gene="nanR"
                     /locus_tag="OO339_003873"
     CDS             complement(4834..>5441)
                     /gene="nanR"
                     /locus_tag="OO339_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5928308.1"
                     /translation="AFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADTIIGE
                     LSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQSLDN
                     NAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVSYQQH
                     IAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1325 a         1528 c         1405 g         1183 t
ORIGIN      
        1 gggcaagcga attaagcgga caggtatata cagtatcaag ggtgttaaca taaaagaata
       61 caacgtcagg taatggcaca gagcggtccg tcagttcagg tttggctctg tgccaaagat
      121 gggtcaggtc acacctgatc taataaattt aagccattaa atcagcctta accttcacca
      181 ccaccttttt aatctcaccc ggctcgccaa caacgcatcc cggcttatgc ggctctcctg
      241 gcataaacac ggcgaacatt cccggtttca gaatgatggt ttgctcgttt tcaatggcgc
      301 tacaaagctg gtaatcatct tcatggtgga actcttcaca ttggcgcgca gtgcctgcca
      361 tgccaaacag aatccgttcc tcaccgttta ataacagctg gatatcaatg tattgctcgt
      421 gcaattccgc ttttttctcg acgggcgatt gagtgttaaa cgtcatgaca ttcataaaaa
      481 tattgtcgcc ctgtaattcg taacgacccg gcgctttttc ttgcggtctg gcagctaagg
      541 ccagcgttaa cgcgtcctgt aacgcaggat gtaacccagc agacggtaat gactgtactt
      601 cacccatcat cataattttt ctccctgggc caacagcgca gccccaagta aacctgcatc
      661 atggcggtaa tgcgccgcca gtaaatcaac atgaaatgcc gctggctcct gcgccagata
      721 catttccacc aacgccagat acccttctgc cagaccaacg ctgccaccaa ccaccacgca
      781 ctggcaatca gttgtggctt taatatcagc gatcagcctt gcaagcgtac gtgcggagcg
      841 atgaatcagt tgctgcgcct gctcatcgcc ctgcccggcg cgtgtgaaaa tagttctcgc
      901 atccgcgcca gccagctcgc cctgcgctgc cgccgcaatg ccgcgaccag aagcaattgc
      961 ttccacgcaa cctgtgcgtc cacagccgca gactgggccg tgtggatcgg caagcgtatg
     1021 cccgatgtgc cccgccagac cgccagggcc ggtaagcagt ttgccgccgc tcactacacc
     1081 gccgccgacg ccagtggaaa cggtgataaa gaccatatcg gttatatcgc cttccagcgc
     1141 ctgatactct gcccatgctg cggcctgcgc gtcgttaatg gcaatggtcg gcaaattggt
     1201 aagttgttcc agcgttttga ctaacggaaa gtgtagcaat ccaccgagat tatgcggatt
     1261 cagcgccagc aaactgccgt cgcgaataat tccggttgaa gcgatggcaa cccgctgcgc
     1321 atgagcttgc aacggagaga ctaatgcgga taaggcatca cgcaaggctt ctggtgtctg
     1381 gctggctggc gtaggaagtt cacgacgatc gcggatctgc ccgtcagcgc caatcagcgc
     1441 ggcggcaagt ttagtaccgc cgatatcaat cgccagtatg gtcatagcac cgcctttttc
     1501 atcgctgtgt tgtaccactg acaaatgtgt tcaagacgcg taatggcaga accgaccgtc
     1561 accgcccacg cgccgtggcg catcgcatct gccgcctgag caggcgtgtt gtaacgccct
     1621 tcggcaatca cccgacatcc ggcttcgctc aacgttttca ccagcgccag atccggctct
     1681 tctggcgttt caggcgtggt atagccagaa agcgtagtgc caataatttc ggctcccagc
     1741 ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg tcatcgccag taaaccgtga
     1801 tgatggatac gtgccagcag cgtttcaaca ggcaccggac gcgggcggtc ggtgccgtca
     1861 atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcga catcttcaat ataggccgtg
     1921 atgcgtaccg gagaatcctc cagatcgcgt ttcacaattc caataatcgg cacgctcacc
     1981 accgcacgcg tggcttgcag atttgccaca ccttcgatgc gaatggcaac cgcgcccgcc
     2041 tgctctgccg ctaatgccat ggctgcgacg atttcgggtt tatcgagcgg gctgtcagga
     2101 accggctggc aggagacaat caggccaccg ttagcagcga ttttttgatc cagttgtgca
     2161 agtaacgaca tacatcttcc cttagcgaaa ggcccggtac atagaccggg caacaggatt
     2221 aacttttggt tttgactaaa tcgtttttgg cgctgccaaa tggcacggca ccgctaaatg
     2281 gtttgccgtc gatagcgtca tgagtacgca atgcttccgg gcgcagccaa cgctgaacgc
     2341 gggaaggcat atccagacca atcagcagga tcaccacaaa tgtcaggctg aacgagagcg
     2401 atgccagcgc agtacccaga tccagacgtt gagcgatcaa cgcgccgatg attggggcca
     2461 gtgcgccacc caatgcgcca acgttgtagg taaagcccag gcccgctgca cgctggtcgg
     2521 tatcgaaata accgccaatc agtttcggta agatcccggc gatcccttgt ccaagcattt
     2581 gctggaagaa cagtaacaga ccgagcaccc agacgtttgc gccgccaatc gcaaataccg
     2641 gaataatcag cagctgcgag gccagcaggc tacaaacgta cgctttgcgg gttcccagcc
     2701 agtcaccgag gaagccacct acgcagcatc ccacggccgc gccaaagcca ctaaagaaca
     2761 gcacattggc gacagtatgc gggttataag ccagatcggt tttcagatac gttggcagca
     2821 gcgcctgaat cggccatgag tagaggaaag caaacaacac gaccaccatc agcattacgc
     2881 ccgttggcca gcgtttaccc gtactttgca ccataaagct gataaagatt gcggcgcata
     2941 acagcccgag aacagcgacg attgcagcat tttgcaggtt accggcgaag cagaaccaca
     3001 gcgcagtagc cgccgccagt gtcattacga tattggcaat gcgatgttca ccacggtaga
     3061 gaatatccac cattgtgcgt actggtgctt tacctgcgtg tttctctttc cagtcttccg
     3121 cttccgggat gtttttacgc agccagagag caaagatgat tggcaaaatg ccgataaaga
     3181 acagcgcacg ccaaccccag accggaacca ccaggctata gacctgagcg gcaacgacgg
     3241 cccccacaga gaagcctgaa atcaaaaaac cactggcttt gttacgcaga tgttttggcc
     3301 agctttcaat gacataggtg gcgctggaac cgtattcacc cgccatcccc atgccgatga
     3361 ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa gccgcaggcc agcgtcccgg
     3421 ccgagaagag aacgatgctg gtgaccattg ccagacgacg cccgtagcgg tcacccatag
     3481 cgccgagcat caggccgccg aaccagcgag agataaaggc tgcggagatc agacttgccg
     3541 cctgcaccgt cgtcagcccg aattcaccct gtacttcggt gagtaccagg gcgattaaaa
     3601 cgaaatcaaa accgtcaagc agatatccca accaggcagc ggaaaatgcg cgccattgtg
     3661 cacggttgag atggcgatac cacgggatat tctgggttgt agtactcatt gtgagtctcc
     3721 cgcggtgggc gatgcccaca cgctttggta tgaaaattgt agggtacaga tgcgtttatt
     3781 tcccctcacc cggtaggggc gagcgagggg aaaacaactc acccgcgctc ttgcatcaac
     3841 tgctgggcca gcgccttcag ttctggcaga tatttttcat ctaccggtcc aaacggtttg
     3901 cggcacagcg gcacagaaac gacatccata taatgcagga cagttttcag gccgcggaat
     3961 acgcccgttt tgatcagtaa atcaatgact ttgttgcact cagtttgcag tttctgcgcg
     4021 gtctggatat cgccttcttt cagcgccttc acgatcccct gatatcgcca gcccatgatg
     4081 ttgtaagtac tgccgatacc accatcagcg cctgccagca agccggaagc gaagatttcg
     4141 tcgtaaccgt tatagagcac cagatcagga tgttcacgac ggatctgttc catctgatag
     4201 agatcgccag aagtctgttt cagcgcaccg acgccaggca acgtaaccag ggtgttgatc
     4261 tgatccaggg tcagttttac cccactcagg gctggaatgt tgtacaccac catcggcaaa
     4321 ccatccgctg aatcaataat tgcccgatag tgatcgcagt gttcttcaaa gctgaaagga
     4381 tagtagaacg gcgtgacggc ggagacggca tcgaagccat aacgtttagc cgatgccgca
     4441 agttgttggc tttcggcggt gctgacgcaa ccgacgtggg cgatgagttt aatcttacct
     4501 ttcgcctctt cggcgacgat ttccagtacc tgttcacgct cggaaaggct ttgtacaaag
     4561 gcttcgccgg tcgaaccacc cacgtataaa ccgtcgatgc cctgctgaat attgaactga
     4621 accaggcggc gcagactcgc tttatccagt gcttgttgtt gatcaaaagg agtcaggagt
     4681 gcagccatta cgccacgtaa attcgttgcc ataaatacct ctgaagtgat gcttgtctga
     4741 taaacgatat acctttatac ctgttatacc agatcaatta agcaacaccc catacagaaa
     4801 gcttataatg cgatctgctt cactaaagtg gcattatttc tttttgttgg tggtctgacc
     4861 gaaagcgtgc caggtagcag agacgctgtt gagatgcgat tgcaacgcac gatcggcttc
     4921 gtcaggatca tgacggcgga tcgcatcaac gatcgcaata tgctgttgat aactaacgtt
     4981 gttatgttcg tgcagtgcct gatcggtaac cgttgggcgt gcggcaataa gccagtcgag
     5041 cagggcaacg tggatcgcca tgaagattgg gttaccgggg atctccgcca gcacgcggtg
     5101 gaaatcaacg tctgaacgaa tgaatgccgc gttgttatcc agcgactgac tgttgatttc
     5161 cagtgctttt gccagcaaat cgatttgctc atcggtggcg tgttcagccg catagcgcac
     5221 caggctggat tcaaagaaca gacgtaattg ttcgaaatgg gcaatcccac caggatgaga
     5281 aaggaaatct ttcgccatgc cggaaagctc gccgatgata gtgtccgcag aaggtcgaga
     5341 gacacgagcg cgttcgccgt tgtttatttg caccagacct ttgcgtttta acgctgccag
     5401 cgcttcacgc acagaaggac gcccgacgtt aaagaacgcc a
//
LOCUS       DAJTRH010000175         5340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTRH010000175 DAJTRH010000000
VERSION     DAJTRH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(36..773)
                     /gene="bamD"
                     /locus_tag="OO339_003874"
     CDS             complement(36..773)
                     /gene="bamD"
                     /locus_tag="OO339_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5928309.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            908..1888
                     /gene="rluD"
                     /locus_tag="OO339_003875"
     CDS             908..1888
                     /gene="rluD"
                     /locus_tag="OO339_003875"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5928310.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1885..2616
                     /gene="pgeF"
                     /locus_tag="OO339_003876"
     CDS             1885..2616
                     /gene="pgeF"
                     /locus_tag="OO339_003876"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5928311.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2746..5319
                     /gene="clpB"
                     /locus_tag="OO339_003877"
     CDS             2746..5319
                     /gene="clpB"
                     /locus_tag="OO339_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5928312.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1458 g         1282 t
ORIGIN      
        1 gctcaagggc tgccgttttg tgtttcaggt ttctgttatg tattgctgct gtttgcggcg
       61 atgattttcg ctactttttc agcttgcgca ttcatctgca tctgacggta tgcattttcc
      121 atcagcggca gcgcatcacg cgtagcctgg gtatccgggt agtcgcgcaa catgccttct
      181 atgcggttaa cgacggcaac ccatgcgcca cgttctgtat agtactcggc cacggagtat
      241 tcatatttcg ccagacgatc tttcaggaat accagacgtt tggtggcatc ggtagtgtac
      301 tgactgttcg gatagccgcg caccagtttg gaaaagtcac taaacgcagc tcgtgcatgt
      361 tgaggatcgc gatcgctacg gtcaacgcca aagaaccctt gcagcgcact gtcatccagc
      421 gccatattgg tcaggccacg catgtacatg acataatcga tattcggatg ggtcgggtta
      481 aggcgaataa aacgatcgat ggcagcctgc gctaacggca aatcggcgtt tttatagtag
      541 gcgtagatga gatccagctg cacctgctgc gaatacggac caaacggata gcgattatct
      601 aacgcttcca gttgcgttat tgcctgtctc cagttaccgt cctgcagctt ttgttgtgca
      661 gtcgcgtaaa tttcatttgg cggattatca ggtacttctt cctttgaccc cgagcaaccc
      721 gccaaaaaca ggcttagtgt ggcggctgcc accagatatt tcatgcgcgt catgacgttt
      781 tgactttcct caaaatgtaa tacgggagat tctctgttcc tgcacccggt taagaccagc
      841 tacaatagca cactatatta aacggcaaag ccgtaaaacc ccaacgataa acgaagaagc
      901 agtatatatg gcacaacgag tacagctcac tgcaacggtg tccgaaaacc aactcggtca
      961 acgcttagat caggctttgg ccgaaatgtt cccggattat tcacgttcgc gaataaaaga
     1021 atggatcctc gaccagcgcg tgctggtaaa cggcaaagtt tgtgataagc cgaaagaaaa
     1081 agtattgggt ggcgagcagg ttgccatcaa cgctgagatt gaagaagaag cgcgttttga
     1141 accgcaggat atccctctgg atatcgtcta tgaagatgaa gatatcatcg tcattaataa
     1201 accgcgcgac ctagtggtac atcctggcgc gggtaacccg gatggcacgg tactgaatgc
     1261 gttgcttcat tactatccgc ccattgccga tgtaccacgt gcgggcatcg tccatcgtct
     1321 ggataaagac accactggcc tgatggttgt ggcaaaaacc gttccggctc agacgcgttt
     1381 agtcgaatct ttgcaacggc gtgaaattac tcgtgagtat gaagcggtgg cgattggtca
     1441 tatgaccgct ggcggtacag tagatgaacc catcagccgc cacccgacca aacgtactca
     1501 catggcggtg catccgatgg gcaaaccggc ggtgactcac tatcgcatca tggaacactt
     1561 ccgtgtgcac acgcgtctgc ggttgcgtct ggaaactgga cgtacgcacc agatccgcgt
     1621 gcatatggcc catatcactc atccgctggt gggcgatccg gtttatggtg gccgtccgcg
     1681 tccgccaaaa ggtgcttcgg aagcatttat ctccacgctg cgtaagtttg accgccaggc
     1741 gcttcatgca accatgctgc gtctttatca cccgatctcc ggcatcgaaa tggaatggca
     1801 tgcgcctatt ccacaagata tggtggagct gattgaggtg atgcgcgccg atttcgaaga
     1861 gcataaggat gaagtggact ggttatgagt aagctgattg tcccgcagtg gccgctgcca
     1921 aaaggtgttg cggcctgtag ctccactcgt atcggcggcg tgagcttgcc cccgtatgac
     1981 tcactcaacc tcggtgccca ttgtggcgat aacccggatc acgttgagga gaatcgcaag
     2041 cgactttttg ctgcgggcaa tttgccttct aaaccggtct ggcttgagca ggtacacggc
     2101 aaagatgtgc ttaagcttac tggcgaacct tatgcctcaa aacgagcaga tgcctcttat
     2161 agcaatacgc ctggcacggt ttgcgcagtg atgactgccg actgcctccc tgtgctgttt
     2221 tgcaatcgag cgggaacgga agtcgccgcc gttcatgctg gctggcgtgg actgtgcgca
     2281 ggcgtgctgg aagagacggt ttcctgtttt gctgataaac ctgaaaacat tctcgcctgg
     2341 ttagggccgg caattggtcc acgcgcgttc gaagtggggg cggaggttcg cgaggcgttt
     2401 atggcagtag acgctaaagc aagtgcagct ttcattcagc atggtgataa gtatctggcg
     2461 gatatttatc agcttgcccg gcagcgtctg gcgaacgtgg gtgttgagca aattttcggc
     2521 ggcgaccgtt gtacatatac ggaaaatgag actttcttct cttatcgtcg cgacaagacc
     2581 accggtcgta tggcaagttt catttggctg atataaccta aagaatcaag acgatccggt
     2641 acgcgtgatt ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc
     2701 tcatttaatc tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt
     2761 cttactaata aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac
     2821 aaccaattca tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg
     2881 gttagtcctt tattaacatc cgctggcata aatgctggcc agttgcgcac agatatcaat
     2941 caggcattaa atcgtttacc gcaggttgaa ggtactggtg gtgatgtcca gccatcacag
     3001 gatctggtgc gcgttcttaa tctttgcgac aagctggcgc aaaaacgtgg tgataacttt
     3061 atctcgtcag aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc
     3121 ctgaaagcaa caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga
     3181 ggtgaaagcg tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc
     3241 atcgacctta ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa
     3301 gaaattcgcc gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt
     3361 ggtgaacccg gcgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac
     3421 ggcgaagtgc cggaaggatt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg
     3481 gtggctgggg cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat
     3541 cttgccaaac aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggc
     3601 gcgggtaaag ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt
     3661 ggtgaattgc actgcgtagg tgccacgacg cttgacgaat atcgccagta catcgaaaaa
     3721 gatgctgcgc tggaacgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagat
     3781 accattgcga ttctgcgtgg cctgaaagaa cgttacgaat tgcaccacca tgtgcaaatt
     3841 actgacccgg caattgttgc agcagcgacg ttgtctcatc gctacattgc tgaccgtcag
     3901 ctgccggata aagccatcga cctgatcgat gaagcagcat ccagcattcg tatgcagatt
     3961 gactcaaaac cagaagaact cgaccgactc gatcgtcgta tcatccagct caaactggaa
     4021 caacaggcgt taatgaaaga gtctgatgaa gccagtaaaa aacgcctgga tatgctcaac
     4081 gaagaactga gcgacaaaga acgtcagtac tccgagttag aagaagagtg gaaagcagag
     4141 aaggcatcgc tttctggtac gcagaccatt aaagcggaac tggaacaggc gaaaatcgct
     4201 attgaacagg ctcgccgtgt gggggacctg gcgcggatgt ctgaactgca atacggcaaa
     4261 atcccggaac tggaaaagca actggaagcc gcaacgcagc tcgaaggcaa aactatgcgt
     4321 ctgttgcgta ataaagtgac cgatgccgaa attgctgaag tgctggcgcg ttggacgggg
     4381 attccggttt ctcgcatgat ggaaagcgag cgcgaaaaac tgctgcgtat ggagcaagaa
     4441 ctgcaccatc gcgtcattgg tcagaacgaa gcggttgatg cggtatctaa cgctattcgc
     4501 cgtagccgtg cggggttggc ggatccaaat cgcccgattg gttcattcct gttcctcggc
     4561 ccaacgggtg tggggaaaac tgagctttgt aaggcgctgg cgaactttat gtttgatagc
     4621 gacgaggcga tggtccgtat cgatatgtcc gaatttatgg agaaacactc ggtgtctcgt
     4681 ctggttggtg cgcctccggg atatgtcggt tatgaagaag gtggctacct gaccgaagct
     4741 gtgcgtcgtc gtccgtattc cgtcatcctg ctggatgaag tggaaaaagc gcatccggat
     4801 gtcttcaata ttctgttgca ggtactggat gatgggcgtc tgactgacgg gcaggggaga
     4861 acggtcgact tccgtaatac ggttgtcatt atgacctcta acctcggttc cgatctgatt
     4921 caggaacgct tcggtgaact ggattatgcg cacatgaaag agctggtgct cggtgtggtt
     4981 agccataact tccgtccgga attcattaac cgtattgatg aagtggtggt cttccatccg
     5041 ctgggtgaac agcacattgc ctcgattgcg cagattcagt tgaaacgtct gtacaaacgt
     5101 ctggaagaac gtggttatga aatccacatt tctgacgagg cgctgaaact gctgagcgag
     5161 aacggttacg atccagtcta tggtgcacgt cctttgaaac gtgctatcca gcagcagatc
     5221 gaaaacccgc tggcacagca aatactgtct ggtgaattgg ttccgggtaa agtgattcgc
     5281 ctggaagtta atgaagaccg gattgtcgct gtccagtaat gataaaacga gcccttcggg
//
LOCUS       DAJTRH010000176         5318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTRH010000176 DAJTRH010000000
VERSION     DAJTRH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..170)
                     /locus_tag="OO339_003878"
     CDS             complement(<1..170)
                     /locus_tag="OO339_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928313.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OO339_003879"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OO339_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5928314.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OO339_003880"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OO339_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OO339_003881"
     CDS             complement(3616..4383)
                     /locus_tag="OO339_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5928315.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5318
                     /locus_tag="OO339_003882"
                     /pseudo
     CDS             4579..5318
                     /locus_tag="OO339_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT         1313 a         1369 c         1380 g         1256 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaagga
//
LOCUS       DAJTRH010000177         5089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTRH010000177 DAJTRH010000000
VERSION     DAJTRH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..654
                     /locus_tag="OO339_003883"
     CDS             <1..654
                     /locus_tag="OO339_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5928316.1"
                     /translation="AGSLQSYKGSLYWNNVTYPFPLTTNTNVLDIGDKTPMPLPLKLY
                     ITPVGAAGGVVIKAGEVIARIHMYKIATLGSGNPRNFTWNIISNNSVVMPTGGCTVDS
                     RNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVG
                     VTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            988..3267
                     /gene="ydeP"
                     /locus_tag="OO339_003884"
     CDS             988..3267
                     /gene="ydeP"
                     /locus_tag="OO339_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5928317.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3515..3718
                     /gene="safA"
                     /locus_tag="OO339_003885"
     CDS             3515..3718
                     /gene="safA"
                     /locus_tag="OO339_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5928318.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3786..4547
                     /gene="ydeO"
                     /locus_tag="OO339_003886"
     CDS             3786..4547
                     /gene="ydeO"
                     /locus_tag="OO339_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5928319.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4949..>5089
                     /locus_tag="OO339_003887"
     CDS             4949..>5089
                     /locus_tag="OO339_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5928320.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1456 a         1083 c         1134 g         1416 t
ORIGIN      
        1 gcaggctccc tacagtcata taaaggatcg ctgtactgga ataatgtgac ctaccccttc
       61 ccgttgacga caaataccaa cgtattggat attggtgata agacaccgat gccattaccg
      121 ttaaaactct atattacccc agttggggcg gccggtggag ttgtcataaa agcgggggaa
      181 gtcattgccc gtattcatat gtataaaatt gcaacattag gaagcggaaa tccgcgtaat
      241 tttacgtgga atattatttc aaataacagt gttgttatgc caacaggcgg ctgcaccgtt
      301 gatagtcgta atgtcaccgt ggatctcccg gactttcctg gaagcgcaga aatacccctt
      361 ggcgtatatt gctcaagcga acaaaagctc tcattttatc tttcgggtgc caccactgac
      421 agtagccgac aagtgtttgc taacacagca cctgatgcaa caaaagcatc gggagtgggt
      481 gtcactctaa tgcgtaatgg aaaaatactg gctacgggag aaaatgtctc cctggggaca
      541 gtaaataaaa gcaaagtacc tctgggttta agcgcaacct atggacaaac aggaaacaag
      601 gtttcagccg gaaccgtcca gtcagtcatt ggtgtcactt ttatctatga ataattgtga
      661 ttttgacctt agtctatgcg ctccattcac gaaaattgcg cggataatta ttttgtgaag
      721 gctattatcc tacacctgta agaaaatccg cgcacaattg aagccagagt cttatttttt
      781 atattggaaa atatgttaat ttaaacacta tgagagatga tgcttaactt atcgtattta
      841 atctattgtt taacgataat agattaaaaa tagaaagaga gtagtccctt actcttatat
      901 atccatgttg gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa
      961 taattcattt ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggtgct
     1021 gcaggtggtt ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata
     1081 cgccatgatg ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt
     1141 tgtgcatggc cagatcctaa gaacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa
     1201 gcaatcgctt gggaagtcac ggataagcag gtaaatgcct ctttctttgc tgagaatacg
     1261 gttcaatcat tacttacctg gggagatcac gagcttgagg ctgcggggcg tctcactcag
     1321 cctttgaaat atgatgccgt cagcgactgt tacaagccat taagctggca acaagctttc
     1381 gacgaaattg gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact
     1441 tcgggccgca cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg
     1501 agcaataact ttcccgactg ctccaacatg tgccatgaac cgacaagcgt gggtttggca
     1561 gcgagtatcg gtgtaggtaa agggaccgtg ttgctggaag actttgagaa gtgcgattta
     1621 gtcatttgca ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc
     1681 gctttagtga aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg
     1741 gagcgattta ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg
     1801 gccagtgcct actacaacgt gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg
     1861 cgcctgttaa ttgagcgcga tgatgctgca agtgccgcag gtcggccctc attgcttgat
     1921 gacgaattta ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgttctcaat
     1981 tccgagtgga aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactg
     2041 gctgacgcat atgccgctgc cgaacgcacc attatctgtt acggaatggg gatcactcag
     2101 cacgaacatg gtacccagaa cgtgcagcaa ctggtcaatt tgctgttgat gaaaggtaac
     2161 attggcaagc ctggtgcggg tatctgccca ctacgtggtc actctaatgt acagggcgac
     2221 cgaaccgtcg gtatcaccga gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc
     2281 tatggcttcc ccccacctca tgcacctgga catgctgcaa ttgccagcat gcaagcaata
     2341 tgtacggggc aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca
     2401 gatcgggaag cgagcgctgt accgttaacg caattagatt tggcggtaca cgtagccact
     2461 aagcttaacc gctctcatct gctgaccgca cggcatagct atattctgcc ggttctgggg
     2521 cgtagcgaga ttgatatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg
     2581 tcgatgattc atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag
     2641 tgtgcagtgg tcgcgggaat cgcgcaggca gcactacccc agagcgtggt agcctgggag
     2701 tatctggtgg aagattatga tcgcattcgc aatgacattg aagctgtgct gccagagttc
     2761 gccgactata accagcgcat ccgtcatccc ggtggttttc acctgataaa tgcagctgct
     2821 gaaaggcgct ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa agggctgtta
     2881 gaagatccct cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagccacgat
     2941 cagtacaaca cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga
     3001 gatgtggtct ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga
     3061 gttaatctta ttgcgcttac gccagacggt aagcgcagct cacgccgcat ggatagatta
     3121 aaagtggtca tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat
     3181 cacatgctaa cacttgataa ccacgatcca ttaagtggta ttcctggcta taaaagtatt
     3241 ccggttgaat tagaaccatc aaattaatgc ctcttctcat ttcttctgct gtcatccgca
     3301 cagcagaaga attcctcatt gactattatt gcgcaatttg ctcacatgga ttaaattaaa
     3361 ctacatacta taagatataa acttctgcct acagctgtaa gaaactccgc tcagtactga
     3421 agcaccagtc ctatttcttc ttttctccag cctgttatat taagcatact gattaacgac
     3481 ttttaacgtt atccgctaaa taaacatatt tgaaatgcat gcgaccacag tgaaaaacaa
     3541 aatcacgcaa agagataact ataaagaaat catgtctgta attgtggttg tcttattact
     3601 gacacttacg ttgatagcca ttttttcggc aattgatcag ctgagtattt cagaaatggg
     3661 tcgcattgca agagatctta cacatttcat tatcaatagt ttgcaagact ggaaataaac
     3721 agcaaattat aaatgtgaaa tgtttaaaag gtatcgataa aactttattg ttttaaggag
     3781 ataaaatgtc gctcgtttgt tctgttatat ttattcatca tgctttcaac gctaacattt
     3841 tagataaaga ttacgccttc tccgacggcg agatcctgat ggtagataac gctgttcgta
     3901 cacattttga accttatgag cggcatttta aagaaatcgg atttaatgaa aataccatta
     3961 aaaaatatct acaatgcact aacatccaga cagtgacggt gcctgttcct gcgaagtttt
     4021 tacgtgcttc aaatgtaccg actggattgc ttaatgaaat gattgcttat ctcaactcgg
     4081 aagaacgcaa tcatcataat ttttcagaac ttttgctttt ttcttgcctg tctatttttg
     4141 ccgcatgcaa aggtttcatt acactattaa ctaacggtgt gctatccgtt tccgggaaag
     4201 tgagaaatat tgtcaacatg aagctggcgc acccatggaa gctgaaagat atttgtgact
     4261 gcctgtacat cagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa acgacattct
     4321 cacagattct attagatgcc agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt
     4381 cagtcaataa aattgccgaa caatgtggtt atgccagtac atcttatttt atttatgcgt
     4441 tccgcaaaca tttcggcaac agtccgaaga gagtttctaa ggagtaccgt tgtcaaaggc
     4501 acacgggtat gaatacgggc aacacgatga gtgctttagc tatttgatta tttgctaacg
     4561 agtagtcaac cacgcacgct acgtaagaat tatatggggc agccattccc tgccccgcgt
     4621 tgtttttagg cgatatattt attgaaataa ataagtgaca tccatcacat atttatgcac
     4681 ttgcataacc tgttgcatga ttatttatga tctcaattcg gcattttgtc agtaaaatgc
     4741 aataatttat taaatatcaa taaattagtt gtttatcggc gagaaattac ttaatagaac
     4801 agaaagtaat gtcaacgctt tatggactgt tatttccctt tttttagcta aatctgctat
     4861 ctctttatgt gactaacttc acttacatcc acttatttct cttcgtaaaa ttacttcgga
     4921 attaagtaca ataagaagag gaacatttat gaagtctgca ttaaagaaaa gtgtcgtaag
     4981 tacctcgata tctttgatac tggcatctgg tatggctgca tttgctgctc atgcggcaga
     5041 tgatgtaaag ctgaaagcaa ccaaaacaaa cgttgctttc tcagacttt
//
LOCUS       DAJTRH010000178         5080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTRH010000178 DAJTRH010000000
VERSION     DAJTRH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..1225)
                     /locus_tag="OO339_003888"
     CDS             complement(<1..1225)
                     /locus_tag="OO339_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5928321.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLA"
     gene            complement(1241..2125)
                     /locus_tag="OO339_003889"
     CDS             complement(1241..2125)
                     /locus_tag="OO339_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5928322.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2136..2741)
                     /locus_tag="OO339_003890"
     CDS             complement(2136..2741)
                     /locus_tag="OO339_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5928323.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(2912..>5080)
                     /locus_tag="OO339_003891"
     CDS             complement(2912..>5080)
                     /locus_tag="OO339_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5928324.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1489 a         1242 c         1044 g         1305 t
ORIGIN      
        1 cggctaaacc tttggcgtgg agatcggcaa cggcttgttt aatcaatatt tgcaaatggg
       61 ttttgcgctg gcgaataccg cgccatccaa cactgttcag gtagattttg tcgattaaac
      121 gcccgaatgc gttactgcct tgcggttgat tttgatagac ataatccagg gaactgcctg
      181 aatcaaagcc ggtactcatt ccgagatgca ggcccgcaga gtgcgcaccg aacaatttca
      241 tcgcctttcg cataaagcga tacgctaaat caaccggcga taatggcacg ggtccaccag
      301 aaaggagccg ccatcgatcc gctgatggtc ctgtgcggtc ttcatgctga taatcaaatt
      361 tttgcgattt gttagcatat aagcggcaga taaagctttg cattttttca aatgcctgcg
      421 ccctgttctc ttcacccaat gtgtcgtgat aaaagcctgg cagcagatgc agctctttca
      481 gagggctacg taatctctga taaaaatcaa tctgcggttg gcgatgcacc acatagtcat
      541 cacctgatat cagaagttgc gtggggagcg taatcgccgc cgcatcgcta acaatacgtt
      601 cagacgtttt atagagatcg agcaagatat tgacggcaat cgcccgtgtg atcagcggat
      661 cattattgaa actcgccacc cgttgccgat cgtgggtcaa atagcgccct ttcacatagg
      721 aattaataaa aaatagacca cgcagacgat gccataacgc cagcgcagga cgtgccagcg
      781 gcacatacaa tttaacctta aaggccggag aagccagcac cagcccacga attgcaggtg
      841 cataatcatg aacccatgtg gcaacaagca ctgcgccgac gctttgcgcg atcactacca
      901 cctcttccag tccgacctgg ctgtcgctgg cagcaaaacg gacaaattca tcgacatcct
      961 gcactgagcg cataagagac gggctataac cacgcggccc cgaatttttt ccatgccccc
     1021 gggcatccca ggcataaaaa gcagtatcag gcatcgccag ttcatcaacg agatgttgta
     1081 gacgaccaga atgttcatgc ccgcgatgaa ataagacgat gacctttttc gcccctggct
     1141 gtaaagtggg ccagtgccga taatacaatg ctgtattatc actggtggta aaaaaatgtt
     1201 ccccagggat gcgtgaattt tccattttct ctattttcct tcagtaacag cagtagcgaa
     1261 taaaataaaa aaataccgga gcggtgaaaa ttaatgagtc aatcctgtca aggagtccgc
     1321 catgtcctag taatagtttt ccactatctt taacaccaat atctcgtttg atggctgaca
     1381 tcactacatc gccgcaaaaa ccactaatac caattaacaa acccgctaat aatgcctgta
     1441 atgtatttag cggtgtcagc aacggtccaa taatcagtga cgcaatcatg gtggtgatta
     1501 cgccccccag cagaccttcc agcgtttttc ccgggctgac tttggggacc acttttcttc
     1561 tgccgcagga tttcccccat aaatactgtg caatatcgtt tgactcggtt aacgccaaaa
     1621 gaaataacac cagtaacgcc ccgccctgga tatttatggt tggcaacatt aatagccagg
     1681 cggcatgact caaggcaaaa acggtcatta tccatcccca aaaaatggcg ctcacggtat
     1741 gctgaaaacc ggaggggtca cccacaaaaa ctcgccaggt ggctaatatc agaaacccag
     1801 tcaaaggtat aaataacaga aacagctcaa agcaattaaa accgataagc aaatagttaa
     1861 gaggaataac ccaatataac caacgcgaaa aatgtacgga tataagcgta caatattctt
     1921 tcaatgccag aaaacttatc agcgtaaaaa acgtcaacgt catccagcgt gggcctgaaa
     1981 tcaccagtga aaaacaaatg acgatgcccc accaggtacg aatacgtaat gtaacttcat
     2041 ccccttttct ctcaggtaac cgccatagca gaaaacggtt tatcagcttg ctaaaattaa
     2101 caacgcgaaa agtgttgcca gagatttttc cagcattatc tttcagcgct cataagaagc
     2161 acactccgac aacgattaat cgcagtacag ataagcagaa ttgaagcaat gctccataaa
     2221 agattattcc attgcatcca ctgtggataa atggcaacgg ccagacccca cagaccaaat
     2281 attaacgcgc gatcactttt gccaaatgga ccggcataac tgcgaacacc gttaatcgtc
     2341 tgcgcgagta aaccgcaaaa ctcggtcaat atggtgcaaa ataacatgag tatcacgagt
     2401 gatgcgttac tttccggtaa aaataaaaac ggtaaataga gcgcaatatc ggaaataaca
     2461 tcgccagtct cattcaaaat cgcccccaga cgtgtttgct gattgcactc acgcgccaac
     2521 atgccatcca gcgcattgag cgccatacgg ataaaaagca cgatgggcaa tagcaaaaag
     2581 aggatgggtt gtgccaccaa caccaacagc aatccggtaa aaagagaaag cgccagtgcc
     2641 gtaagagtga tgtgattcgc tgtaacgtgg tgcttataaa gccaaaacat cgtcggcctt
     2701 aacagcgact gaaagagcgg ttttatctgg tagagcgtca ttatcaatcc ttgataattc
     2761 ggtttgcgct atggtgcatc aattcccctg acggagatat cgcaacacgc cgcttcatta
     2821 aaaattcaga atatatcaag gtattggggg ctcactgtca ggaatgatta tccccaataa
     2881 atcaagtagt taaaaattat tacgctgtta ttcaatacaa ctcatcaagt ttcctcatcg
     2941 cttgttcccg tattgcgcgc gtcttaccgt taagcgccag ctggcggact aaatccagtt
     3001 cagggttaac aagctctaac gccagcaaag gatcacgttt cagcctcgcc ttcaatacat
     3061 caactggaaa gcggggatta ttcatcgcca caatccacca ctcgggatca atttctgccg
     3121 ccagtgccgc agagggggtg tggcggttgc taagtgcgcc gaacttgaaa aatgtgagat
     3181 tgcttttcgc cagtgtactc caccatatac gatccggttg ttttgccagt acatccagtt
     3241 gtgctaacgc atcggggtcg agtgcataat aaacacgtaa tgcttcatca acctgcgcac
     3301 gggagctatt ttcctgcctc agaaaaaact tcgcccaaat atcattgcgt ggcgtgtttt
     3361 gctgtaattc cttgagaaca acagcgggta aattatccca actaactaat gcactctcta
     3421 aaaagtccca gtcttccaaa cgtatcaggg cggcggcatt taatagcatc tggtcatcaa
     3481 cttcgccgtg gctaatcagc tcagacaaaa tcatatcagc gctggtttca tatgcatctg
     3541 aatctgcttt cggattattt ttccgatgcg ataaagcacg ttgccaaatt ttttgtttca
     3601 tcgacgtgct cagatactga tttttcgcca gattactcca cccctcagga tcgttgaaac
     3661 gtgtcaataa ccgttgtaaa gcagcgtcag agtgaattac ctgcatatcc cgacagcaaa
     3721 agtaggcagg ggattttttc tccatccttt taacaaaatt aaactgtaag ttatctggca
     3781 atgctaaaaa taacacacgt attagatcat cgtcattctc gaatttttgg tataaatagg
     3841 ctgcaacttt gtcactctct tgtggcgtca tccgcaataa ttgaccatgg tgtttcatct
     3901 ctgccagctt gaacgctaag cttgtgacaa cattgtttga tgaatcgttc gccaactgca
     3961 ttgcctgatt gtaagtgagt tgtcctccag caccgacctg acgaatagag gaatcatcac
     4021 tgcggatata gccatcaata atggattcgg gcaataacca cgcatcttta taaagctcct
     4081 gaatgatcat atcccaatct cttttcgcca gagtcatgac gatatccaga gggagatttt
     4141 cacgtagcgc aagattcaac ggaccgacac catcaggtag acgactcatt cgcgtcatta
     4201 tttcgacaga gagcggttca cggtcgagca accccagaac aacaggctgg taacccaatt
     4261 gctgatcggt caactccaga atctcattgt caggcatata tctcgccata ctttcaatcg
     4321 tttgttcatc attgctatta ataagttgtt tgcgtaggtt agctgaaatt cctttaagcc
     4381 ttgggagagt tcgcattatg gcaagatcaa atgtcgcaat atctcgtcct tcaaatgcag
     4441 caacgttgcc ttcatccagc atttgcaatt cctgcactaa ctcaggagaa ggcttagggc
     4501 gaaacagcgc ttcttttttc atctgattac agacattccc tgccatgcga atattgcttc
     4561 gcttaaaaat ttctttcaga gcatcttcat catttattag accccaataa tatgcatcag
     4621 gatttattat catcagcgct tttttgacat tttcagtgtc atttttgtca ctcgcacgga
     4681 ccatgaattc actatttgtc gaagacgcaa caacaggtgc aattatgtca ttgataaata
     4741 ccccacgcgc attaatactc cccttaatcg tagacggatt atcgtaatat gagtcgataa
     4801 ataccagtcc acttgcattt atcgaaccgg taattaacag tggtgaatcc gtaactatat
     4861 tcgttgccgt cacgttaccg agaataaccc atggattaga aagagagaaa gtggaaatat
     4921 tacctttgac tgtcaaattg ccaagaacaa tcaatttttg attactctct atattacctt
     4981 ctaccactaa atcgccacga tgcactttcc agtccgatgc agataaaccg aaggggacaa
     5041 gattcacgct actgttgata gcttgttcaa cttgttgttt
//
LOCUS       DAJTRH010000179         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTRH010000179 DAJTRH010000000
VERSION     DAJTRH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            245..429
                     /locus_tag="OO339_003892"
                     /pseudo
     CDS             245..429
                     /locus_tag="OO339_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            572..793
                     /locus_tag="OO339_003893"
                     /pseudo
     CDS             572..793
                     /locus_tag="OO339_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(814..876)
                     /locus_tag="OO339_003894"
                     /pseudo
     CDS             complement(814..876)
                     /locus_tag="OO339_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1326..2738
                     /gene="ospC1"
                     /locus_tag="OO339_003895"
     CDS             1326..2738
                     /gene="ospC1"
                     /locus_tag="OO339_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5928325.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2842..2991)
                     /locus_tag="OO339_003896"
                     /pseudo
     CDS             complement(2842..2991)
                     /locus_tag="OO339_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3067..4764
                     /gene="ospD3"
                     /locus_tag="OO339_003897"
     CDS             3067..4764
                     /gene="ospD3"
                     /locus_tag="OO339_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5928326.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4881..5000
                     /locus_tag="OO339_003898"
                     /pseudo
     CDS             4881..5000
                     /locus_tag="OO339_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1714 a          927 c          953 g         1430 t
ORIGIN      
        1 tctccaggat tcccggggcg gttcaatcaa agcgcagaac ttctgcgtct tcttcgtcaa
       61 aaaaatcatc ttcgtgaagc ttcacgacat agcggggaag tttgcttctt tgagaggcgg
      121 gtttacgttt acgggattta gctgaacggg ccatataacc acctgaaaga caatgacatt
      181 gcctgttttt ataacggtaa ttgcagacca tgacaaaccg cagccgtcag gctgcctact
      241 cgagtcacaa ggctctttgt gtccgatatg ccggcagcgc attacccatg aaactggctg
      301 gaacgtacac cataaggtga agaaagttat gggggcggtg aagaattatc taaccttgtc
      361 ttgttacatc caaattgcca tcgacagtta cacagttgtg aagccggctc tctcaaaaag
      421 ggcttataaa ggcttgagcg gtgtaccggg aaaccggtat gcaccgttct taggggaggg
      481 cagtccagca atgaactgtc cttacccgac aaacatccaa aacgaacgga acgtgctgga
      541 atccgcatat aacccgttgt aaagtgagga aatggacacc tcacttgctc atgagaacgc
      601 ccgcctgcgg gcactgttgc agacgcaaca ggacactatc caccagatgg ctgaatacaa
      661 ccgcctgctc tcacagcgga tggcggctta tgcttccgaa atcaaccgac tgaaggcgct
      721 ggttgcgaaa ctgcagcgta tgcagttcgg taaaagctca gaaaaacttc gcgcaaaaac
      781 cgaacggcag attccatttt cacgggcgat ctacgccaca caggctctgg gttgcagtga
      841 ctgctccatt aaggcgattt tgaactcata agggaagtaa caaaaaaact gcaaaagtac
      901 aataattata tatggccaaa ctaacctcta ttaaaaaaca tcagtttttc acacttagtt
      961 gagcttcatg aaaaaatcat cgaggatttc attgcagaat acacattcca ctaactggaa
     1021 gcactccata agaggaatca gagaaagtat cgttttttta tagtaaaatt cattgctgtt
     1081 caataaaatt gatatagtta ttaaattatg aaaataattt cacaaaaaag attaacttga
     1141 gttcttaagc atcggatttt agatattttg aaatatctgg catcacataa catcaaaagt
     1201 atataatact cttatctaat tacatgcagt atattgtata tatactcaaa aattgtaatt
     1261 cataccctgc tatatcttgt agggaaagat attaattaaa actgttttca tataaggttc
     1321 attttatgaa tatatcagaa acactgaact cagcaaatac ccaatgcaat atagattcta
     1381 tggataacag attacataca ttgtttccaa aagtgacatc agtgcgaaac gctgcacaac
     1441 aaactatgcc agatgaaaaa aatttaaaag atagtgcaaa tattattaaa agtttcttta
     1501 ggaaaactat agcagcacag agttatagta gaatgttctc tcaaggctct aactttaaat
     1561 ctttaaatat agcaattgat gcaccatcag acgctaaagc ctcatttaag gctattgagc
     1621 accttgacag attatcgaag cattatatat ctgaaataag ggaaaaactt catcctcttt
     1681 ctgcagagga actcaatttg ctttcgctaa ttattaattc tgatttaatc ttcagacatc
     1741 aaagtaattc tgatttgtct gataaaattt taaacattaa gtcattcaat aaaattcagt
     1801 ctgaaggaat atgcacaaaa cgaaacacat acgctgatga tataaaaaaa atagctaatc
     1861 atgactttgt gttttttggc gttgaaatct ctaaccatca gaaaaaacac cccctgaata
     1921 caaaacatca cactgttgat tttggtgcaa atgcgtatat cattgatcat gactctccat
     1981 atggatatat gacattaacc gatcactttg ataatgctat tccacctgtt ttttaccatg
     2041 agcaccaatc atttttagat aaattttcag aggttaataa agaagttagt cgatacgtac
     2101 atggaagtaa aggaattata gatgtaccaa tattcaatac taaagatatg aagttagggc
     2161 tcggattata cctgattgac tttattagaa aaagtgaaga ccaaagcttc aaggagtttt
     2221 gctatggaaa aaatcttgcc cctgtggatc tggatagaat cataaacttt gtttttcagc
     2281 cagagtacca tatacctagg atggtaagta cagaaaactt caaaaaagtt aagattagag
     2341 aaatatcctt agaggaggct gttacagcat ctaattacga agaaattaac aagcaggtca
     2401 ctaacaaaaa aattgctctc caggctcttt ttctttcgat tactaatcaa aaagaggatg
     2461 tcgccttata tatattatct aattttgaga taactagaca agatgttatt tccataaagc
     2521 atgagttgta tgatattgag tatctactta gcgctcataa ttcaagctgt aaagtacttg
     2581 agtattttat caataaggga ttggttgatg taaacacaaa gttcaaaaaa actaatagtg
     2641 gggattgtat gttggataac gcaataaaat atgagaatgc agaaatgata aaactattat
     2701 tgaaatatgg tgcaacatct gacaataaat atatttaatc aaaattgaat atcgtttagc
     2761 aaaaggcagt atttgcacaa aatatcgata tattgtttat tgtcagtatg gctcaatgtg
     2821 ataatagttg gaaagtttga tgggtttcgc cccgttgtag cggtagtcga actggaaggt
     2881 cttcaggcac tgcttacagc gatagagcag cccccctgaa ctggaatggc cgttccggat
     2941 acccccctga gtttcagagt aacggtgaca aaccacatca atctttgcca tcaatcatcc
     3001 aaagggcaaa gagtacaaca acactaagtc tgcgtcacaa cccatcaatg aaaggaatat
     3061 atacatatgc catcagtaaa tttaatccca tcaaggaaaa tatgtttgca aaatatgata
     3121 aataaagaca acgtctctgt tgagacaatc cagtctctat tgcactcaaa acaattgcca
     3181 tatttttctg acaagaggag ttttttatta aatctaaatt gccaagttac cgatcactct
     3241 ggaagactta ttgtctgtcg acatttagct tcctactgga tagcacagtt taacaaaagt
     3301 agtggtcacg tggattatca tcactttgct tttccggatg aaattaaaaa ttatgtttca
     3361 gtgagtgaag aagaaaaggc tattaatgtg cctgctatta tttattttgt tgaaaacggt
     3421 tcatggggag atattatttt ttatattttc aatgaaatga tttttcattc cgaaaaaagc
     3481 agagcactag aaataagtac atcaaatcac aatatggcat taggcttgaa gattaaagaa
     3541 actaaaaatg ggggggattt tgtcattcag ctttatgatc ccaaccatac agcaactcat
     3601 ttacgagcag agtttaacaa atttaactta gctaaaataa aaaaactgac tgtagataat
     3661 tttcttgatg aaaaacatca gaaatgttat ggtcttatat ccgacggtat gtctatattt
     3721 gtggacagac atactccaac aagcatgtcc tccataatca gatggcctga taatttactt
     3781 caccccaaag ttatttatca cgcgatgcgt atgggattga ctgagctaat ccaaaaagta
     3841 acaagagtcg tacaactatc tgacctttca gacaatacgt tagaattact tttggcagcc
     3901 aaaaatgacg atggtttgtc aggattgctt ttagctttac aaaatgggca ttcagataca
     3961 atcttagcat acggagaact cttggaaact tctggactta accttgataa aacggtagaa
     4021 ctactaactg cggaaggaat gggaggacga atatcgggtt tatcccaagc acttcaaaat
     4081 gggcatgcag aaactatcaa aacatacgga aggcttctca agaagagagc aataaatatc
     4141 gaatacaata agctgaaaaa tttgctgacc gcttattatt atgatgaagt acacagacag
     4201 atacccggac taatgtttgc tcttcaaaat ggacatgcag atgctatacg cgcatacggt
     4261 gagctcattc ttagcccccc tctcctcaac tcagaggata ttgtaaattt gctggcctca
     4321 aggagatatg acaatgttcc cggacttctg ttagcattga ataatggaca ggctgatgca
     4381 atcttagctt atggtgatat cttgaatgag gcaaaactta acttggataa aaaagcagag
     4441 ctgttagaag cgaaagattc taatggttta tctggattgt ttgtagcctt gcataatgga
     4501 tgtgtagaaa caattattgc ttatgggaaa atacttcaca ctgcagacct tactccacat
     4561 caggcatcaa aattactggc agcagaaggc ccaaatgggg tatctggatt aattatagct
     4621 tttcaaaata ggaattttga ggcaataaaa acttatatgg aaataataaa aaatgaaaat
     4681 attacacctg aagaaatagc agaacacttg gacaaaaaaa atggaagtga ttttctagaa
     4741 attatgaaga atataaaaag ctgaatatta tctcgtggtg atacactgaa tttgaggtaa
     4801 ccggctcatt taaaccgtct ggtctgttac ctccggttcc acaaaaataa tgtccatcat
     4861 tttgaagtgg acactatcgt atggaatacc ggacctggat cactgaagct ttacgccttc
     4921 atttcgaaga acatttacca cgggttgtgg ccggacgtcg cctgggcgta ccaaaatcaa
     4981 ctgtctgtgg tatgttcgtg tgaaccgccc ccggtatccc gctc
//
LOCUS       DAJTRH010000180         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTRH010000180 DAJTRH010000000
VERSION     DAJTRH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            49..123
                     /gene="tnaC"
                     /locus_tag="OO339_003899"
     CDS             49..123
                     /gene="tnaC"
                     /locus_tag="OO339_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5928327.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            344..1759
                     /gene="tnaA"
                     /locus_tag="OO339_003900"
     CDS             344..1759
                     /gene="tnaA"
                     /locus_tag="OO339_003900"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5928328.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1850..3097
                     /gene="tnaB"
                     /locus_tag="OO339_003901"
     CDS             1850..3097
                     /gene="tnaB"
                     /locus_tag="OO339_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5928329.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3229..4404
                     /gene="mdtL"
                     /locus_tag="OO339_003902"
     CDS             3229..4404
                     /gene="mdtL"
                     /locus_tag="OO339_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5928330.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4379..>5024
                     /gene="yidZ"
                     /locus_tag="OO339_003903"
     CDS             4379..>5024
                     /gene="yidZ"
                     /locus_tag="OO339_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5928331.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1255 g         1417 t
ORIGIN      
        1 ttctgagtgt aataatgtag cggcgtgtct ttcgaggata agtgcattat gaatatctta
       61 catgtatgtg tgacctcaaa atggttcaat atcgacaata agattgtcga tcaccgccct
      121 taatttaccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatcgatt
      181 agtttgctat atcttaattt tgccttttgc aaaggccatc tctcgtttat ttacttattt
      241 tagtaaatga tggtgcttgt atatatacat ggtgaattaa tggatattgc agatgtaata
      301 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
      361 tctccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta ctcgcgctta
      421 tcgtgaagaa gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
      481 gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta tgcaggcagc
      541 gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt tagccgagtc
      601 agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc gtggcgcaga
      661 gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc tggatcgcag
      721 caaaatggtg gcattctcta actatttctt tgataccacg cagggccata gccagattaa
      781 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
      841 ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag ttggcccgaa
      901 taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc agccggtttc
      961 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc cggtggtaat
     1021 ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
     1081 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
     1141 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
     1201 cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag gcttcccgac
     1261 atatggcggc ttggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt atgacggcat
     1321 gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg atggtctgga
     1381 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
     1441 actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt gcgagctgta
     1501 taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
     1561 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
     1621 atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa aagagaacgc
     1681 ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
     1741 aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta gccactctct
     1801 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
     1861 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaattgg
     1921 tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct ggggtgcctt
     1981 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
     2041 tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa taggtaacac
     2101 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
     2161 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc
     2221 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
     2281 ttcgttagcc gccagtcgca ttacctcatt gttcctcggg ctgaagatta tctcctttgt
     2341 gatcgtgttt ggttctttct tcttccaggt cgattactcc atcctgcgcg atgccaccag
     2401 caccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
     2461 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
     2521 taagttaatc aaaagcgtgg tatttggttc gctgctggcg ctggtgattt atctcttctg
     2581 gctctattgc acgatgggga atattccgcg cgaaagcttt aaggcgataa tctcctcagg
     2641 cggcaacatt gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga
     2701 gttttgcctg ctggtgttct ctaacttagc tgttgccagt tcgttctttg gtgtcacgct
     2761 ggggttgttc gattatctgg cggacctgtt taagattgat aactcccacg gcgggcgttt
     2821 caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg tatctgatct tcccgaacgg
     2881 ctttatttac gggatcggcg gtgccgggct gtgcgccacc atctgggcgg tcattattcc
     2941 cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat cagatgttca cggtctgggg
     3001 cggcaatctt attccggcga ttgtcattct ctttggtata accgtgattt tgtgctggtt
     3061 cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaagaag ccagccattc
     3121 gctggcttct tgcctctcag gaaatcactt atgtccaaat ggcaactcgc cttatcctcc
     3181 ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt
     3241 ttgatttgta gttttgccct ggttttactt tatcccgccg ggattgatat gtacctcgtt
     3301 ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttgca tattgcgttc
     3361 tccgtatatc tggcggggat ggcagctgcg atgctatttg ccggtaaagt ggccgatcgt
     3421 tcagggagaa agccggtcgc catacccggc gcggcgctat ttattattgc ctcggtgttc
     3481 tgttcactgg ctgaaaccag cacgttattt cttgcaggcc gatttctaca ggggttgggc
     3541 gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg acacgctgga tgatcgacgt
     3601 cgggctaaag tgctgtcatt actcaacggt attacctgca tcattccggt gttagcgcca
     3661 gtgctcggac atctgattat gcttaaattc ccgtggcaga gtctgttctg ggcgatggca
     3721 atgatgggca tcgcggtact gatgttgtct ttgtttattt taaaagaaac gcgcccagcg
     3781 tcccccgctg cttcggacaa accacgagaa aatagcgagt cgctgcttaa tcgttttttc
     3841 ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacttt cgtcaataca
     3901 tcgccggtat tgctgatgga aatcatgggg tttgagcgcg gagaatacgc caccattatg
     3961 gcgctgaccg ctggcgtcag catgaccttt tcattctcga cgccatttgc gctgggaatt
     4021 tttaagccac gtacgttgat gatcacctcg caggtgttat tccttgcggc ggggatcacc
     4081 cttgccgttt caccttccca tgcggtttct ctgtttggta tcacgctgat ttgcgccggt
     4141 ttctcggtag gttttggcgt agcgatgagt caggcgttag gaccattttc attacgcgcg
     4201 ggcgtagcca gctcgacctt aggtattgcg caggtttgcg gttcgtcact gtggatttgg
     4261 ctggccgcgg tggttggtat cggcgcatgg aatatgctga tcgggattct gattgcctgt
     4321 agcatagtga gcctgttgct gattatgttc gtcgcgcctg gacgccccgt tgccgctcat
     4381 gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac tgctgatgca
     4441 ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg accccttcag cggtgagtaa
     4501 gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgctggg
     4561 gctgtcgccc acacagctaa tggtcagcat ggagcaaaat ctggcggaat ggatgcaaat
     4621 gagcaatctg ctgctggata aaccgcacca ccagacaccg cgcggcctga agtttgagtt
     4681 ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg ctgtcgaaac ggatctacca
     4741 acgttacccg caggcgacca tcaaactacg taactgggat tacgattcct tagatgccat
     4801 tactcgtggt gaagtggata ttggtttttc cggtcgcgaa agccatcctc gctcgcggga
     4861 gctgttaagc tcgctaccgt tagccattga ttatgaagtg ctgtttagtg atgtaccctg
     4921 cgtctggtta cgcaaagatc atccggcact gcatgaaacg tggaatctgg acaccttttt
     4981 acgttatccg catatcagca tttgctggga acagagcgac acct
//
LOCUS       DAJTRH010000181         5022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTRH010000181 DAJTRH010000000
VERSION     DAJTRH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..642
                     /locus_tag="OO339_003904"
     CDS             <1..642
                     /locus_tag="OO339_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5928332.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="OO339_003905"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="OO339_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5928333.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..>5022
                     /gene="hrpA"
                     /locus_tag="OO339_003906"
     CDS             1502..>5022
                     /gene="hrpA"
                     /locus_tag="OO339_003906"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5928334.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRV"
BASE COUNT         1221 a         1251 c         1403 g         1147 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagatc
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagatat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gt
//
LOCUS       DAJTRH010000182         4974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTRH010000182 DAJTRH010000000
VERSION     DAJTRH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO339_003907"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO339_003907"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5928335.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OO339_003908"
     CDS             1020..1703
                     /locus_tag="OO339_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5928336.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OO339_003909"
     CDS             1700..2161
                     /locus_tag="OO339_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5928337.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OO339_003910"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OO339_003910"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5928338.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OO339_003911"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OO339_003911"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5928339.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO339_003912"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO339_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5928340.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO339_003913"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO339_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5928341.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1177 a         1305 c         1239 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tacg
//
LOCUS       DAJTRH010000183         4915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTRH010000183 DAJTRH010000000
VERSION     DAJTRH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            180..437
                     /locus_tag="OO339_003914"
                     /pseudo
     CDS             180..437
                     /locus_tag="OO339_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO339_003915"
     CDS             complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO339_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5928342.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO339_003916"
     CDS             complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO339_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5928343.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO339_003917"
     CDS             complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO339_003917"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5928344.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3062..3205)
                     /locus_tag="OO339_003918"
                     /pseudo
     CDS             complement(3062..3205)
                     /locus_tag="OO339_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3595..4410)
                     /locus_tag="OO339_003919"
     CDS             complement(3595..4410)
                     /locus_tag="OO339_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5928345.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4507..>4915
                     /locus_tag="OO339_003920"
     CDS             4507..>4915
                     /locus_tag="OO339_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5928346.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTE"
BASE COUNT         1303 a         1121 c         1167 g         1324 t
ORIGIN      
        1 cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat tagctaacta
       61 atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag acaacggtga
      121 aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag gattacatca
      181 tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg
      241 ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt atagaacagt
      301 acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca tcgatgattt
      361 ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg
      421 gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa
      481 gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt
      541 aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa
      601 tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg cctgtaaatg
      661 ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc
      721 attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc cacgaatggc
      781 atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac
      841 cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt
      901 ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac
      961 cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct
     1021 tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc
     1081 atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat
     1141 ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag
     1201 ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat
     1261 agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg
     1321 caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc
     1381 tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca
     1441 gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt
     1501 gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga
     1561 cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg ccagcaagct
     1621 gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc agcataccgc
     1681 ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac
     1741 caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa
     1801 aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca
     1861 acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct
     1921 gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga atttcacata
     1981 ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa
     2041 atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga
     2101 ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag
     2161 ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct
     2221 gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct
     2281 ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca
     2341 taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt
     2401 aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc
     2461 tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa caagctggca
     2521 caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg
     2581 ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg
     2641 tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg
     2701 gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg
     2761 tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc
     2821 aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac cacctgggtt
     2881 ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat
     2941 gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac
     3001 ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct
     3061 cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga taccacgcca
     3121 gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact aacgaatagt
     3181 aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga ttttttaatt
     3241 tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga taattccagt
     3301 cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata gccgccagac
     3361 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
     3421 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
     3481 gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt gttataacgg
     3541 ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata taagttaaat
     3601 catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt taccgggcga
     3661 gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat agcccagacg
     3721 atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg
     3781 catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag
     3841 tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg
     3901 gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc
     3961 aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa
     4021 acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag
     4081 taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact
     4141 ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg
     4201 cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc
     4261 agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg
     4321 ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg
     4381 tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca
     4441 aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa
     4501 agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg
     4561 actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat
     4621 gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct
     4681 gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat
     4741 gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc
     4801 aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt
     4861 gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaac
//
LOCUS       DAJTRH010000184         4779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTRH010000184 DAJTRH010000000
VERSION     DAJTRH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            268..4386
                     /gene="pic"
                     /locus_tag="OO339_003921"
     CDS             268..4386
                     /gene="pic"
                     /locus_tag="OO339_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5928347.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4537..>4779)
                     /locus_tag="OO339_003922"
     CDS             complement(4537..>4779)
                     /locus_tag="OO339_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR5928348.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1285 a         1086 c         1263 g         1145 t
ORIGIN      
        1 gtataacaat cgacgattgc tggaaaggct gggccatatc ccaccggcag aagcagaaaa
       61 agcttattat gcttccatcg gaaatgatga tctggcagcc tgagttcaca gataaaacac
      121 tctccaggaa acccggggcg gttcagttca caaaaacaca ttaatgcagt aactatattt
      181 tcctttctgg tgataacgtc gggttatcat tagcttcttc agctatttta cttttatatc
      241 ccttgtaaac atcatggaga atccatagtg aataaagttt attctcttaa atattgcccc
      301 gtcaccgggg ggcttattgc tgtctctgaa cttgcccgca gggtaataaa aaagacatgc
      361 cgaagattaa cgcatattct tctggctggc attccagcaa tctgtctgtg ttactctcag
      421 atatcccagg cgggtattgt ccgttccgat attgcctatc agatttatcg tgatttcgcc
      481 gaaaacaaag ggctttttgt acctggtgcc aatgatattc cggtatatga taaggacgga
      541 aaacttgtgg gaagactggg taaagcccca atggccgatt tcagcagtgt gagctcaaat
      601 ggcgttgcta cgcttgtatc gcctcagtat atcgtcagcg taaagcataa cggaggatat
      661 cggagtgtga gctttggtaa tgggaaaaat acatattccc ttgttgaccg taataaccac
      721 ccttctattg acttccatgc tccacgtctg aataaactgg ttacagaagt tattccctca
      781 gcggtaacat cagaaggaac caaagccaat gcttataaat acactgaacg ttacaccgct
      841 ttttatcggg tgggtagtgg tacgcagtac actaaggaca aggacggaaa tttagttaag
      901 gttgccggtg gatatgcttt taaaacagga ggaaccacag gagttcctct gatatctgat
      961 gcaacaatag tctctaatcc cgggcaaact tataatcctg taaacggccc tttacctgac
     1021 tatggagccc ctggggatag tggttctcct ttgtttgctt atgataaaca acaaaaaaaa
     1081 tgggttattg ttgctgtatt aagagcatat gcaggtatta atggtgctac gaactggtgg
     1141 aatgtcatac caacagatta tctgaaccag gttatgcagg acgatttcga tgcccccgta
     1201 gactttgttt ccggactggg ccccctgaac tggacatacg acaaaacatc aggcacaggt
     1261 accctgagcc agggcagtaa aaactggacc atgcacgggc agaaagacaa tgacctcaat
     1321 gccggtaaaa atctggtatt cagcgggcag aatggtgcaa ttatcctgaa agacagtgtg
     1381 actcagggtg ccggttatct cgaatttaaa gacagttaca ccgtatctgc tgaatccgga
     1441 aaaacatgga cgggtgccgg cattattact gacaagggga cgaatgtaac ctggaaggtc
     1501 aacggcgttg ccggtgacaa cttgcataag ctgggggaag gaaccctgac cataaacgga
     1561 acaggtgtaa acccgggagg actgaaaacg ggagacggta tcgttgtact taaccagcag
     1621 gcagacactg caggtaatat ccaggccttc agttcagtga acctcgccag cggacgtccg
     1681 accgtggtgc tcggggatgc ccgtcaggtc aatccggata acatttcatg gggataccgg
     1741 ggaggtaagc ttgaccttaa tggtaatgcc gttaccttca cccgactgca ggctgctgat
     1801 tacggggcgg tgattacaaa taatgcacag caaaaatccc agcttttact ggatcttaag
     1861 gctcaggata caaatgtcag tgaaccgacg attggaaata tatccccctt tggtggtacc
     1921 ggcactccag gaaacctgta cagcatgata ctcaacagcc agacccgctt ctatattctg
     1981 aaatctgcca gctatggtaa cactctgtgg gggaacagcc tgaatgatcc ggctcagtgg
     2041 gagtttgttg gcatgaacaa aaacaaagca gttcagacag taaaagatag gatcctggcc
     2101 gggcgggcaa aacaacccgt tatctttcat ggtcagctga ccgggaatat ggatgtcgcc
     2161 attccacagg tgccgggggg aagaaaggta atctttgatg gtagcgtgaa cctgccggaa
     2221 ggtaccctga gtcaggacag tggcaccctg atattccagg gacatccggt tatccatgcc
     2281 tccatcagtg gcagtgcacc ggtcagcctg aaccagaaag actgggaaaa ccgtcagttt
     2341 acaatgaaaa cactgtcgct gaaagacgct gacttccatc tttcacgtaa cgcctcgctg
     2401 aacagtgaca ttaagtcgga taacagccat atcacactgg gaagtgacag ggcatttgtg
     2461 gataaaaatg acggaacagg aaattatgtc attccggagg aaggtacctc tgtcccggac
     2521 accgtgaatg acaggagcca gtatgaaggg aatattacgc tgaaccataa ctcagccctg
     2581 gatatcggca gcaggttcac cggggggatt gacgcttatg acagtgccgt cagcatcacc
     2641 tctccggacg tcctgttgac agccccgggt gcttttgccg gcagttcact gacagtgcat
     2701 gatggcggtc atcttacagc actgaacggt cttttcagcg acgggcatat tcaggccggt
     2761 aagaacggca aaatcaccct gagcggtaca ccggttaaag atacggctaa tcagtatgcc
     2821 cctgctgtat atctgacgga cggatatgac ctgaccggcg ataacgcagc actggaaatt
     2881 acccgtggag cacatgcttc cggtgatatt catgcctctg cggcatcaac agttaccatc
     2941 gggtctgaca cgccggcaga actggcttct gcggaaacgg ctgcatcggc gtttgccggc
     3001 agtcttcttg agggctataa cgcagcattc aatggtgcca taaccggtgg cagggctgat
     3061 gtcagtatgc ataatgcact gtggactctg ggtggggact ctgccatcca cagtcttacc
     3121 gtcagaaaca gccgtattag ttctgaagga gaccgtacat tccgtaccct gacggtgaat
     3181 aaactggatg caacaggcag tgattttgtt ttgcgtacgg acctgaaaaa tgccgataaa
     3241 attaatgtga ctgaaaaagc cactggttca gataacagcc tgaacgtcag ctttatgaat
     3301 aatcctgctc agggacaggc cctgaatatt cctctggtca cggcaccggc gggaacttca
     3361 gcagagatgt ttaaggccgg cacccgggtg acaggtttca gtcgggtgac cccaaccctg
     3421 catgttgata ccagtggtgg caatacgaag tggatactgg atggttttaa agcggaggct
     3481 gataaagccg ctgccgcgaa ggctgacagt ttcatgaatg ccgggtataa aaacttcatg
     3541 acggaagtta acaatctgaa caaacgtatg ggtgacctgc gtgacacaaa cggtgatgcc
     3601 ggtgcctggg cgcgcatcat gagtggtgcc ggttctgcag acggtggtta cagtgataat
     3661 tacacccatg ttcaggtcgg ctttgacaaa aaacatgaac tggacggtgt ggacctgttt
     3721 accggtgtca cgatgaccta taccgacagc agtgcagaca gccatgcatt cagcggaaag
     3781 acgaaatcgg tggggggcgg tctgtatgct tcagcattgt ttgagtccgg tgcctatatc
     3841 gatttgattg gtaaatatat tcaccatgac aatgattaca caggtaactt tgctagcctg
     3901 ggaacgaaac actacaacac ccattcctgg tatgccggtg ctgaaacggg ttaccgctat
     3961 cacctgacag aggacacgtt cattgagccg caggctgaac tggtttacgg cgccgtgtcc
     4021 gggaaaacat tccgctggaa agacggtgat atggacctga gcatgaagaa cagggacttc
     4081 agtccgctgg ttggaagaac aggggttgaa ctgggcaaga ccttcagtgg taaggactgg
     4141 agtgtgacgg cccgtgccgg aaccagctgg cagtttgacc tgctgaataa tggagagacc
     4201 gtactgcgtg atgcgtccgg ggagaaacgg ataaaaggag agaaggacag ccggatgctg
     4261 tttaatgttg gtatgaatgc gcagataaag gacaatatgc gctttggtct ggagtttgag
     4321 aagtcagcct ttggtaaata taacgtggat aatgcggtaa acgcgaattt ccggtatatg
     4381 ttctgatgtg ggggtaaagg gctgccggaa ggcagccttt ctcagagact cttaaaatat
     4441 aaaactgaag gtcttcggta agggggagtg tattagacgt caactagttt tgccgactgg
     4501 cgcgagcctg tttaagattc tgtgtaaatg cctttctcag aagtgaccgt ccaggcggtc
     4561 accgaactcg ataataaagc ggctcattgc cattcgccag tccttcaacg gcatcgtcca
     4621 tttctgggac gcagactgga ttgccagcca caccactttt ttcaccgagt cgtctgtcgg
     4681 gaacacttta cgctttttga tcgcatggcg gatcacgctg tttagcgact cgatggcatt
     4741 cgtcgtatag atcactttgc ggatgtccgt tggataagc
//
LOCUS       DAJTRH010000185         4769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTRH010000185 DAJTRH010000000
VERSION     DAJTRH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO339_003923"
     CDS             complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO339_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5928349.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO339_003924"
     CDS             complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO339_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5928350.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO339_003925"
     CDS             complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO339_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5928351.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO339_003926"
     CDS             complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO339_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5928352.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4144..>4769)
                     /locus_tag="OO339_003927"
     CDS             complement(4144..>4769)
                     /locus_tag="OO339_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5928353.1"
                     /translation="TEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVDFEKG
                     IVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFYHREY
                     GRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHTFACW
                     LLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1263 a         1167 c         1124 g         1215 t
ORIGIN      
        1 tgaaggaaga gctgaatagt ctgaaggatt tgaattcggt taggaatttt atcgtggaat
       61 aacagaagct gcggcacgtc gtatgcagga acgtgccgcg gttggctggt aaactttcga
      121 tagtgcgagt attgaatgat ttctaggcgt tatcgatttt acgtattttt tgcatgatag
      181 aattcgtacc tcctcccaac gaccttccat ggctttccgg acttctgtag gctggcggac
      241 gatgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggcgtttc
      301 cctttgtgcg tgcgttgatg ctggtaacgg taaacgtcat gatgaacacg accgcgaata
      361 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacagccag acaccagtca
      421 ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag cgttgtgtca
      481 ggagtactgc aatggcgcca ggcgcaatga gcagagaaat agagagaatg atacctactg
      541 ctttcagcgt cgccacaatg gtcagggaaa ccatgcacag cagcccataa tgcagccatc
      601 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
      661 gccattttac gctaataaca agtgtaacca gcccggcaat aatcaccgtc tggattatgt
      721 cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt
      781 taattgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccataa
      841 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
      901 ctccggcgac gaatgcccct gtagccagag gtaaccccag aatccaggta agcacgatgc
      961 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
     1021 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
     1081 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt
     1141 atgcgcacgc cgacgatttg ccaaaagtcc atgggtgggc gcaaatacaa atgctaaaag
     1201 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
     1261 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatta tgagcaggcg
     1321 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
     1381 cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
     1441 gaggattttt aatctgcccg gatgtaatcc aatagcacgg gcgtgattct cgtcgaaaaa
     1501 ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat
     1561 agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg
     1621 aatgttcacg gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga
     1681 gaagatcagg ccgatgatgg catcttcttt taagcgggtg cgttggttaa gaaacagcat
     1741 gctgcccgct gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat
     1801 atatgccccc gcaacacccg ggacaataga gtgcgacagc gcatcgccaa tcagcgacca
     1861 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca ttgccgagac
     1921 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatcttgct
     1981 tccctctgca ccgctgacgg tcgatgtgcc acaaaagggc gttcatcgtc agtaataatg
     2041 ctttcttccg agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg
     2101 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
     2161 accgtataat cgcaaaatgt cgtgactgag ccgagattgt gcgttgagac aagcatcgtt
     2221 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tcgcttcggt tttcacatca
     2281 acgccggtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat tgctctcgcc
     2341 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgacgatg gcgaaaatcc
     2401 accatatcga cacgttccag cgcatcggtg acaatctgac gatccctctt tttggctata
     2461 cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga
     2521 aatgaccagt caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc
     2581 tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata
     2641 atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
     2701 ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca
     2761 gtaacatcat taacgacaat gcctgcagat tgcatcatta tttctccctt ttcccggctt
     2821 taattccctg taccagcgta ctggtagtaa ccttaagaag gtcgatatac gtaggtaccg
     2881 ggccgttttc tgtgctcagg gaatcgacat agagcacacc gccgtagtgt gcgccggttt
     2941 cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga
     3001 tatgattttt cttaactata tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat
     3061 cggcattaat cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa
     3121 aagccccttc actggtgacc atccatcgct gattctcagg gagttccgta atctgcttac
     3181 gcaggggggc aagggtttgg gtaatcttgg ctttataagt atcggcatta cgttggtagg
     3241 tttgtgcatt tgccggatcg tattttatca acgcatcacg aatattatcg acgtaaatca
     3301 gagcattatc tggcgacatc catgcatggg gattaggttt gccctcatag ggtccttcgg
     3361 tgatccctac tggcgtcaca cccgaagaga caattacttc tggaacccca ttgagatgct
     3421 ggtaaaagcg ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgccc
     3481 cctgcgcacg tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccaggct
     3541 tggttatgga tgagacttct gcagcatctc cagccacgtt tttggccata tccgcgatga
     3601 tggtaaatgt tgtaatgacc ttgaattttt cagttgcact tgcctgaaat gcgatcgagc
     3661 aggtgagggc gaggcacccc aagagcatgg ttactttttt tattgagtgc ataatttagt
     3721 acctatagtg agcaaaatta gaacaaacat agcaacggct atgttttata gcataaccta
     3781 tacaaaatga aaataattat cattaacagc tttgaggcct aaaacaggga ttaggttgtt
     3841 tcctgctggc gacatgatat tgaggaaaaa agtagagcag ctattaggct ttgaaatgac
     3901 tttatgagga aaattgttga tggcaaaaat ggggcaaaaa agagtgggtt atcgtagctt
     3961 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgagtcaga
     4021 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttctttg taatttgttg
     4081 attttcttgc attatttcag ttctctggta ctaaatgggg caaattgggg gcaaactttg
     4141 caactacgat aaccgcgcat tcaacatggc tatctgttcg tcgttcatgt catcaatcca
     4201 cataccgtaa atttcataca ccatttgcgc agtttcatgc cccatctggc tggctataaa
     4261 tgccgggttc gctcctgccg tcaacagcca gcaggcaaaa gtatgtcgcg tatggtacgg
     4321 attacggcgg cgaataccag cacgttttac tgctgcattc catcttgtac ccaaactgct
     4381 taccgagtaa taaggtttct gtttttcgtt acacaccctg ggcatgaaaa caaaatgcag
     4441 tttttgtttt tcagttctgc cgtactcccg atgataaaaa gtgatttcgc ttttgcgatg
     4501 atgcccggtc agtttgtatt gctccttcag tgcttcaaga gccggctgca gtaatgctac
     4561 cgtccggatc ccggcatttg tttttggggg accgaacata tcaagtatcg tcaggtttct
     4621 tctgacattc acaattccct tctcgaaatc cacatcctcc cacgccagag ctgccagttc
     4681 cccgtgacga agcccggagt aaacggcaaa tttccacaag ttttggctct gtcctttttc
     4741 actttccatt aatgcattga attctgttt
//
LOCUS       DAJTRH010000186         4593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTRH010000186 DAJTRH010000000
VERSION     DAJTRH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(69..857)
                     /gene="phoH"
                     /locus_tag="OO339_003928"
     CDS             complement(69..857)
                     /gene="phoH"
                     /locus_tag="OO339_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5928354.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1464..2729)
                     /gene="efeB"
                     /locus_tag="OO339_003929"
     CDS             complement(1464..2729)
                     /gene="efeB"
                     /locus_tag="OO339_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5928355.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2735..3862)
                     /gene="efeO"
                     /locus_tag="OO339_003930"
     CDS             complement(2735..3862)
                     /gene="efeO"
                     /locus_tag="OO339_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5928356.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3920..>4593)
                     /locus_tag="OO339_003931"
     CDS             complement(3920..>4593)
                     /locus_tag="OO339_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5928357.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1076 a         1295 c         1108 g         1114 t
ORIGIN      
        1 agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc tctgcaccga
       61 taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc
      121 tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca
      181 cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata
      241 accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc agtcacattc
      301 tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat ataggcgaac
      361 ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa ggaagcccct
      421 aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt ttctgcgata
      481 tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt gacgataatc
      541 ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat ccaggttttt
      601 ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc ctgcagatag
      661 tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata
      721 ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc ttcacgctgc
      781 ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc tttgatcact
      841 gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca cgcgccgttg
      901 gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc atagtttgct
      961 tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt aacacgagta
     1021 aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag aaatctccgc
     1081 gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc atccgcgatc
     1141 tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa gtacaacaga
     1201 actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc atttcggtga
     1261 cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag ctgtgtgctt
     1321 tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc ggcgcgaacg
     1381 ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc gataaacctg
     1441 cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag ataatcgttc
     1501 gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt aacgtattcc
     1561 tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc cagatcgtgt
     1621 tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac ccccaacgaa
     1681 taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg gggattcgcc
     1741 agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc gtaatcaggc
     1801 acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg gccaaaaatc
     1861 gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg aatcaagcgt
     1921 actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc cgttacccac
     1981 accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc gtctttgaaa
     2041 cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc ggaaataaac
     2101 ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat atcgcgcagc
     2161 gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc accatgacat
     2221 aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt ttttggcatc
     2281 tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa cgtgatggtg
     2341 agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg caggcgcgga
     2401 tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg agtcaacaag
     2461 cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc caccagcatc
     2521 attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa cggctgtttc
     2581 tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg tgcatgagcg
     2641 accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg tgacggttca
     2701 ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc acaccgcgaa
     2761 gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc cggtcggcat
     2821 cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc agaatggtat
     2881 cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct ttttgcagtt
     2941 gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg aaatcccaca
     3001 gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc acttcctcaa
     3061 tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca ctgatgcgtt
     3121 tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc cctttggtgg
     3181 tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt gggtcggcgg
     3241 ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg gagaacagtt
     3301 cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt gctttcgctt
     3361 tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc accagctgcg
     3421 tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga cttaacagcg
     3481 catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc cctttcgggt
     3541 tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc gtcattttct
     3601 ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct ttgaggatct
     3661 cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc ccggcgttaa
     3721 ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc tgcggcacat
     3781 cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac tgcaatgcgt
     3841 tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg taactaagca
     3901 agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg cgtggtggca
     3961 gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact tcgctgacgc
     4021 tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc gagtgagttg
     4081 agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat ccggcttcat
     4141 gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata aacaggctgg
     4201 tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc aggaagccga
     4261 gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc ccgacatctt
     4321 gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca acggcaaaaa
     4381 agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg ctatcgactg
     4441 cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa accatccagg
     4501 taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc tctttttgcg
     4561 gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DAJTRH010000187         4578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTRH010000187 DAJTRH010000000
VERSION     DAJTRH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..1844
                     /gene="cyoB"
                     /locus_tag="OO339_003932"
                     /pseudo
     CDS             1..1844
                     /gene="cyoB"
                     /locus_tag="OO339_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            1834..2448
                     /locus_tag="OO339_003933"
     CDS             1834..2448
                     /locus_tag="OO339_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5928358.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGVTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            2448..2777
                     /locus_tag="OO339_003934"
     CDS             2448..2777
                     /locus_tag="OO339_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5928359.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            2789..3679
                     /gene="cyoE"
                     /locus_tag="OO339_003935"
     CDS             2789..3679
                     /gene="cyoE"
                     /locus_tag="OO339_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5928360.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            3863..4174
                     /locus_tag="OO339_003936"
     CDS             3863..4174
                     /locus_tag="OO339_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5928361.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4158..4316
                     /locus_tag="OO339_003937"
     CDS             4158..4316
                     /locus_tag="OO339_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5928362.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
BASE COUNT          979 a         1132 c         1218 g         1249 t
ORIGIN      
        1 cctccgtcga ccataaacgc ctcggtatca tgtatatcat cgtggcgatt gtgatgttgc
       61 tgcgtggttt tgctgacgcc attatgatgc gtagccagca ggctcttgcc tcggcgggtg
      121 aagcgggctt cctgccacct caccactacg atcagatctt caccgcgcac ggcgtgatta
      181 tgatcttctt cgtggcgatg cctttcgtta tcggtctgat gaacctggtg gttccgctgc
      241 agatcggcgc gcgtgacgtt gcgttcccgt tcctcaacaa cttaagcttc tggtttactg
      301 ttgttggcgt gattctggtt aacgtttctc tcggcgtggg cgaatttgcg cagaccggct
      361 ggctggccta tccaccgcta tcgggaatag agtacagtcc gggagtcggt gttgattact
      421 ggatatggag tctccagcta tccggtatag gtacgacgct taccggtatc aacttcttcg
      481 ttaccattct gaagatgcgc gcaccgggca tgaccatgtt caagatacca gtatttacct
      541 aggcatcact gtgcgcgaac gtactgatta ttgcttcctt cccaattctg acggttaccg
      601 tcgcgttgtt gaccctggat cgctatctgg gcacccattt ctttaccaac gatatgggtg
      661 gcaacatgat gatgtacatc aacctgattt gggcctgggg ccacccggaa gtttacatcc
      721 tgatcctgcc tgttttcggt gtgttctccg aaattgcggc aaccttctcg cgtaaacgtc
      781 tgtttggtta tacctcgctg gtatgggcaa ccgtctgcat caccgtgctg tcgttcatcg
      841 tttggctgca ccacttcttt acgatgggtg cgggcgcgaa cgtaaacgcc ttctttggta
      901 tcaccacaat gattatcgcc atcccgaccg gggtgaagat cttcaactgg ctgttcacca
      961 tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg gactatcggt tttatcgtca
     1021 ccttctcggt gggcggtatg actggcgtgc tgctggcagt accgggcgcg gacttcgttc
     1081 tgcataacag cctgttcctg attgcgcact tccataacgt gatcatcggc ggcgtggtct
     1141 tcggctgctt cgcagggatg acctactggt ggcctaaagc gttcggtttc aaactgaacg
     1201 aaacctgggg taaacgcgcg ttctggttct ggatcatcgg cttcttcgtt gcctttatgc
     1261 cgctgtatgc gctgggcttc atgggcatga cccgtcgttt gagccagcag attgacccgc
     1321 agttccacac catgctgatg attgcagcca gcggtgcagt actgattgcg ctgggtattc
     1381 tctgcctcgt tattcagatg tacgtttcta ttcgcgaccg cgaccagaac cgtgacctga
     1441 ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac ctcttccccg cctccgttct
     1501 ataactttgc cgtagtgccg cacgttcacg aacgtgatgc attctgggaa atgaaagaga
     1561 aaggcgaagc gtataaaaag cctgaccact atgaagaaat tcatatgccg aaaaacagcg
     1621 gtgcaggtat cgtcattgca gctttctcca ccatcttcgg tttcgccatg atctggcata
     1681 tctggtggct ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct
     1741 tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc
     1801 atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg
     1861 actgcccacg cgcacgaaca cgggcaccac gatgcaggcg taaccaaaat cttcggattt
     1921 tggatctacc tgatgagcga ctgcattctg ttctcaatct tgtttgctac ctatgccgtt
     1981 ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt
     2041 ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc ggctatcgcc
     2101 atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggt
     2161 gccggattta tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggg
     2221 ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca cggtctgcac
     2281 gtcacttctg gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg
     2341 accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg
     2401 gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta
     2461 ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt
     2521 cgatcattct gacggtgatt ccgttctgga tggtgatgac aggggctgcc tctccggccg
     2581 taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct
     2641 tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg
     2701 tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca
     2761 acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa
     2821 accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa
     2881 gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc
     2941 gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac
     3001 gaagaatcgg gtgctggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac
     3061 cttgctgggt attgctggct ttatgctgct gtggtttggc gcgaatccgc tggcctgctg
     3121 gctgggggtg atgggctttg tggtttatgt cggcgtctat agcctgtaca tgaaacgcca
     3181 ctcagtctac ggcacattga ttggttcgct ctccggcgct gcgccgtcgg tgattggcta
     3241 ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc ctgctggcta tcttcagcct
     3301 gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt accaggcggc
     3361 aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc acatcacgct
     3421 gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt acgctgggta
     3481 taaatatctg gtggtcgccg cggcggttag cgtctggtgg ttaggtatgg ctctgcgcgg
     3541 ttataaagtt gctgatgaca gaatctgggc gcgcaagctg ttcggcttct ctatcatcgc
     3601 catcactgcc ctctcggtga tgatgtccgt tgattttatg gtaccggact cgcatacgct
     3661 gctggctgct gtgtggtaac aaaacctctc tattaaaaag gtgctacggc acctttttat
     3721 tgtctgagat cagagatacg gctaataacc gtatctcttg acagagtgag cagatgcaca
     3781 taaattaatc atacggttga tagccgtatt atgtatgaga tcgactccct acgatttcat
     3841 tcagttaatc aaagaagtca taatgcctgg aaaaacagcc gctctatacg atgtcgataa
     3901 aacgctgaaa aatgcgcggg tcgaactcaa aactagtccc gacgccaaaa acaaacttcg
     3961 tgaagctgcg caagccgttg gggtagattt aagcgctttt attttgagcg ctgctatgga
     4021 acgcgccgaa agtgtgcttg ataaccaacg tcgccgtgag ctttcgaatc aaagctggga
     4081 actgatgaac caactcatcg ctgaacctgc tcaaccgacg ctcgccctca aggcgttaat
     4141 gaaaaggaaa aacagcgatg gtcgacaagc atgaagagat tactctgccc atagtcctct
     4201 cctgtaatta tcagtctgat attacttatc ctgggcaaaa acagtttgat tgcggtaacc
     4261 ctgttatcga taaatttgta cgcgctacgc gcatcgctaa agaaaagtgt gcgtaatagc
     4321 gactgtgcgg ctaaagcact tattgacaga caaagtggtg aactgattgg catctgtact
     4381 tttacggcat attcgctgga aagacaacgc gtttctggcg tccttcaggg ttcacaacct
     4441 tcagaaattg gtgttgtcag attagtcatg ttggggggag cgcggaagta tcaaaagcgg
     4501 ggctttggtc aggacctact atgtgatttt tttgaacatg taaaaaaaat tcacccggca
     4561 ttaccaatta aaggggtt
//
LOCUS       DAJTRH010000188         4532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTRH010000188 DAJTRH010000000
VERSION     DAJTRH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..1222
                     /locus_tag="OO339_003938"
     CDS             <1..1222
                     /locus_tag="OO339_003938"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5928363.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1222..1956
                     /locus_tag="OO339_003939"
     CDS             1222..1956
                     /locus_tag="OO339_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5928364.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2423..3184
                     /locus_tag="OO339_003940"
     CDS             2423..3184
                     /locus_tag="OO339_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5928365.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3228..4307
                     /locus_tag="OO339_003941"
     CDS             3228..4307
                     /locus_tag="OO339_003941"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5928366.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4304..>4532
                     /locus_tag="OO339_003942"
     CDS             4304..>4532
                     /locus_tag="OO339_003942"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5928367.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSIS"
BASE COUNT         1243 a          862 c         1118 g         1309 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttgca aatataggtg attttttatt
     2221 gtaggctttt gaaggctatt ataactgtgc gctaaattaa tgcgttgaat aaaagaaata
     2281 acaaaattaa gaaaagccat atttacgata tgggcaacat aaatataatc taaaaaagag
     2341 taaggcgttg taggttggtt ccgcgttcat taccttgtta gtgaacgcgg atttatcagt
     2401 tgtcgaaatg gtttttataa taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg
     2461 cttgtcgttt ttatcaagcg catttcccac ctatgctgcg gatgatgtca aagtgactca
     2521 aaacacttca gtttttaaac tgaagcttag tgcaacgcga ttaatctatg aacaagggtc
     2581 ggatggcgca gcgataactg tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa
     2641 agtctttgaa gaagataaag taactgcagc gccctttatt gtcactccgc cactgttcag
     2701 gcttaatcca ggccagcaaa ataaaatgtg ggttgtgcag actgcgccag ctttacaaga
     2761 cagggaaaaa ttacactggc tatgcgccac cggcattacg cctaaaaaga gtgatgtttg
     2821 ggccgagaaa gataaagaaa aaattgctga aaatgtgaac ctgaacatcc aggttaacgt
     2881 acatacctgc ataaaactta ttaccaggcc atctggtatt gaaggcaaga tggttaatgc
     2941 gagtgagaag ttatcgtgga aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc
     3001 gttctacatc aacctttctt ctttacaggt tggcggtagc aagattgaga aactagatta
     3061 tattgcgccg ttttcgcaac atgagtttgt tatgccaaaa actagcggcc aggatattaa
     3121 atggtctgtg attactgatg aaggtggtga gagtcgtttg tttatatctc atttgcaatc
     3181 ataatgtatt acatttttat tgaatatttt tatgccatgc atattccatg ttttatcgac
     3241 tgtcattaat tttgataatg gccttacttg cgggtcagtt aagtgcccag gagtggtcat
     3301 ttgattcttc acagttggaa ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg
     3361 gagagcaact ccccggaaat tatcgtgtgg aaatttatct taacggtgaa aaagttgatg
     3421 ttggggagtt tccctttcat cgtccggaat cccctgaaga aaaagagctt gttccatgtt
     3481 taaccgttga tgaccttatc cactatggca ttaaaattga taaaagcagc agtgatactg
     3541 acaataaaaa gaatcagtgt tttaaatgga actctattga aggtttgaaa gtaaattatg
     3601 attttgatag ccagcgcgta cagataaccg ttccacaact gtatctgcaa gataaaaaaa
     3661 gtagtttagc accagtgtcg ttatggaatg agggtgtagc tgcattccgc atggtgtatc
     3721 agacaaatat tgatatttcc aaacaaaatg ataatcaatc taccacgcgt aattctcgtt
     3781 atggcaggtt cactccaggg tttaatcttg gtgcctggag gtttcgtagt tcagtgacat
     3841 ggagcaagga acttggccag tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag
     3901 gcataaatgc gattaaaagc cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt
     3961 ttgacagcat accttttcgc ggcgggatgt tagcgaccga cgatgcgatg actccgccgg
     4021 aggacagcta ctacacacct gtggtgcatg gaatagcaca aagtgaggcg caggttatta
     4081 ttaaacaaaa tgggcaaatt atttttaccc gttctgtgcc gccagggcct tttgcgctgg
     4141 ataatttacc aacgctggcc gttggcgggg agcttgatgt tacagtgaga gagagtaatg
     4201 gcgaggagca gtatttttcc gtgccatttc aaacgccggc catcgctctg catgaaggat
     4261 atttcaaata tagtgtgatg ggggggaata taaagaagaa ggtataaaaa gctcccctgt
     4321 ctccgtgggc gaaatcacgg cgatgtacgg gcttccttat ggcgtcacgg tgtatggagg
     4381 gattcaaaac gccactcatt ttaatgcaat atctacgggt attggcattt ctctggggct
     4441 gttagggagt ttatcgacag atattacgcg ttcgattgcg aatttatatt atgggaataa
     4501 atatcgtatt cgttatagta aatccattag tg
//
LOCUS       DAJTRH010000189         4526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTRH010000189 DAJTRH010000000
VERSION     DAJTRH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..755
                     /gene="tauA"
                     /locus_tag="OO339_003943"
     CDS             <1..755
                     /gene="tauA"
                     /locus_tag="OO339_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5928368.1"
                     /translation="GDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            768..1535
                     /gene="tauB"
                     /locus_tag="OO339_003944"
     CDS             768..1535
                     /gene="tauB"
                     /locus_tag="OO339_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5928369.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1532..2359
                     /gene="tauC"
                     /locus_tag="OO339_003945"
     CDS             1532..2359
                     /gene="tauC"
                     /locus_tag="OO339_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5928370.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2356..3207
                     /gene="tauD"
                     /locus_tag="OO339_003946"
     CDS             2356..3207
                     /gene="tauD"
                     /locus_tag="OO339_003946"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5928371.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3313..4287)
                     /gene="hemB"
                     /locus_tag="OO339_003947"
     CDS             complement(3313..4287)
                     /gene="hemB"
                     /locus_tag="OO339_003947"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5928372.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1008 a         1160 c         1311 g         1047 t
ORIGIN      
        1 ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca gccagccaac
       61 aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa gcgctggtgg
      121 taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc gtaccgttta
      181 tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt aaaccagggc
      241 tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag cggggtgata
      301 ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac ggcaaggtgc
      361 tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc tgggtggtgc
      421 gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa agcgccatcg
      481 atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg gaaaacatca
      541 gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg gtgaagggaa
      601 atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg aacaaagcaa
      661 tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta gcgaatgatt
      721 acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg ctgcaaatct
      781 ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac ctgacgctgg
      841 aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc accctgctga
      901 atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg ggtaagcgta
      961 ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg ctaccgtggc
     1021 gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag aaaatgcagc
     1081 gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca gaaaaacgct
     1141 atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc gcgctggcgg
     1201 cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc ttcacccgcg
     1261 accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag gtgctgttga
     1321 ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg ctttcatccg
     1381 gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt gttgcgggag
     1441 agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa tatgttttaa
     1501 gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg aaaaactgca
     1561 ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa gcattggcac
     1621 gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga ttagcccgct
     1681 gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg gaccgcaggg
     1741 atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca ttgtgctggc
     1801 gctactcgcg gcggtggtga tcggtattcc ggtcgggatc gccatggggc taagcccgac
     1861 ggtgcgcggc attctggatc cggtaatcga actttatcgt ccggtgccac cgctggctta
     1921 tttgccgctg atggtgatct ggtttggtat tggtgaaacc tcgaagatct tactgatcta
     1981 tttagcgatt tttgcgccgg tggcgatgtc ggcgctggca ggggtgaaaa gcgcgcagca
     2041 ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt gcgcaggtgc tgtggtttgt
     2101 cattttgccc ggcgcgctgc cggaaatcct caccggatta cgcattggtc tgggtgtggg
     2161 ctggtctacg ctggtagcgg cggagctgat tgccgcgaca cgcggtttag gatttatggt
     2221 tcagtcagcg ggtgaatttc tcgcgactga cgtggtactg gcggggatcg cggtgattgc
     2281 gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgtc tgacgccctg
     2341 gcatggagaa gtacaatgag tgaacgtctg agcattaccc cgctggggcc gtatatcggc
     2401 gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt tgaacagctt
     2461 taccatgcgg tgctgcgcca tcaggtggta tttctgcgcg atcaagctat tacgccgcag
     2521 cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt ttaccctcat
     2581 gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc gccggataac
     2641 gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc gattctggca
     2701 gctaaagagt taccttcgac cggcggtgat acgctctgga ccagcggtat tgcggcctat
     2761 gaggcgctct ctgttccctt ccgccagctg ctgagctggc tgcgtgcgga gcatgatttc
     2821 cgtaaatcat tcccggaata caaataccgc aaaaccgagg aggaacatca acgctggcgc
     2881 gaggcggtcg cgaaaaatcc tccgttgcta catccggtgg tgcgaacgca tccggtgagc
     2941 ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgtcga tgtcagcgag
     3001 aaagagagcg aagccttgtt aggcttttta ttcgcccata tcactaaacc ggagtttcag
     3061 gtgcgttggc gctggcaacc gaatgatatt gcgatttggg ataaccgcgt gactcagcac
     3121 tatgccaatg ccgattacct gccacagcgg cggattatgc atcgggcgac gatccttggg
     3181 gataaaccgt tttatcgggc ggggtgatac gagtgcggac ggttccctcg cccccttggg
     3241 gagagggtta gggtgagggg gcgttcactg tactttcaac aggttaactc cccctttctg
     3301 agaggaaaaa aattaacgca gaatcttctt ctcagccaaa tccagcgcaa agtagctgaa
     3361 aatcagatcc gcacccgcac gcttaattga acctaagctt tcgagcacga ctttctcttc
     3421 atctatagca cccgccagcg cggcgaactt aatcatcgcg tactcaccgc tcacctgata
     3481 cgcgccaatc ggcaattcag tacgttcacg cagctcacgc acgatgtcga ggtacgctcc
     3541 agcaggttta accatcaggc agtctgcgcc ctgggcttca tccagcagtg actcacgaat
     3601 cgcctcgcga cggttcattg ggttcatctg atagcttttg cggtcgcctt ttaatgcgct
     3661 tcctgcagct tcacggaacg ggccataaaa ggaagaggcg aacttggtcg aatacgacat
     3721 aatcgccgta tctttaaagc ctgcagcgtc cagcgcctga cgaatcgcct gtacctggcc
     3781 gtccatcgcg gcggaagggg cgatgaagtc tgcgcctgca gcagctgcaa ccacggcttg
     3841 cttgcctaaa ttttccagag tcgcgtcgtt gtcgacgcca tgctcgcaca gcacaccgca
     3901 gtgaccgtga gaagtgtatt cacagaaaca agtgtctgac atgacgatca tttctggcac
     3961 agtctgcttg cagatgcgcg acatacgcgc caccagtcca tcttcccgcc aggcgtcgct
     4021 gccggtttca tcggtatggt gagagatgcc aaaagtcatt acggaacgga taccggcgtt
     4081 ggcgatgcgt tcaatttcgc gtgccagatg tttctctgga atgcgcatca cgcctggcat
     4141 ggcttcaacg gctttgtagt cgtcaatttc ttcttcaaca aagatcggca acaccaggtc
     4201 gttaaggcta agtgttgtct cttcaaacat agcgcgcagc gcaggagatt tgcgcaggcg
     4261 acgagggcgt tggattaagt ctgtcatggt ctgcctgatg tttgtggaat taaggtgcac
     4321 agtatacctg aagcggggca ggagtgtttt acgaaagttg tcgcgatgtt gacaagaaga
     4381 gaatgaaaga gagaccagga catctttgta atagaaatag caataatttc atattcactg
     4441 aaaatatttt taatctttat ttatagctct tggttattat tttttggcgt ttggttgtgc
     4501 tgctataaat tgaataattc aaactt
//
LOCUS       DAJTRH010000190         4503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTRH010000190 DAJTRH010000000
VERSION     DAJTRH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            278..457
                     /locus_tag="OO339_003948"
                     /pseudo
     CDS             278..457
                     /locus_tag="OO339_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(487..969)
                     /locus_tag="OO339_003949"
                     /pseudo
     CDS             complement(487..969)
                     /locus_tag="OO339_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1225..2205)
                     /locus_tag="OO339_003950"
     CDS             complement(1225..2205)
                     /locus_tag="OO339_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5928373.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(2205..3404)
                     /locus_tag="OO339_003951"
     CDS             complement(2205..3404)
                     /locus_tag="OO339_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5928374.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(3726..4064)
                     /locus_tag="OO339_003952"
                     /pseudo
     CDS             complement(3726..4064)
                     /locus_tag="OO339_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4131..>4503)
                     /gene="istB"
                     /locus_tag="OO339_003953"
     CDS             complement(4131..>4503)
                     /gene="istB"
                     /locus_tag="OO339_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5928375.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT         1213 a         1049 c          995 g         1246 t
ORIGIN      
        1 gagtactacg gaaagttctg gtccagaaat ttcaactatc gactgtggag tgcaatgcag
       61 tcacgtctgc tcaagtggat gcagtctaaa tacagacttt cgaaccggaa ggctcagcga
      121 aagctgacgc tggtaaggaa ggagtatccg aagctatttg ttcactggta tttactacgt
      181 gcatcaaatg agtgatcaag agccgtatga cgcgagagtg tcacgtacgg ttccgtgagc
      241 agccggggag tgaaattctc tccggctgac tcgactcgcg ggttccgaca gcgatgacga
      301 acatgcggcg gtgttgtact cgctgatcgg cacatgccgt ctgaacaatg tggaaccaga
      361 aaaatggctg cgttacgtca ttgagcatat ccaggactgg ccggcaaacc gggtacgtga
      421 tctgttgccc tggaaagttg atctgagctc tcagtaaata tcaatacggt tctgacgagc
      481 cgcttacttt tggcgtaatt ctgctgcgcc tttactgaac acaatgccgt attcagcaag
      541 aagtccacga atgtgattta tcagttgtgt cctttccatt gaaaccaacc gacgcatatt
      601 caaaaaagtc tggacgtcct gttgctccag agtttttatt ggtacgggtc gtatagttcc
      661 atgttggcag gcctcagcta ttgcctgtgc gtcgttataa tcatttttct ggcaccgctg
      721 ataagcttta acatgttgtg ccggaagcaa tattacatca tggtcgagtt tcctgatttc
      781 ccgggcccag taatgagatg ccccgccagc ctccatcgca acagtcattt taggctgttg
      841 caccagccag ttaagcagtt gattgcgaga aagtttggtc tttcctgcgg gtttgccatg
      901 agcattgata ctgaaaagat aaaagttcgt tttagccaga tcaatgccgg tgattttctg
      961 ttcagacatg gtatcccgct tacttttgtt gttaaagttt atccatcatg gcgcaattga
     1021 aggctgccgt gaaagagggg gaagactatc tcatcggtta ctgttaaacg gctaagagaa
     1081 actatctttt tcaagcaaag aagatacggt gaaacaggcg atttcaccgt gaaatactgt
     1141 tcaaaaaaaa cgctaataaa agcaaaattt cttttaaaac agccaattaa aaactggcga
     1201 gttgctggcg aaaaacttga attttcacag cgaattcaga ttatctttta gtacaagagc
     1261 aatcatacga tctaacttat cctgaacttc caacggtaat ctgccgaatt catatgagaa
     1321 cgtacgtccc tttactcgtt ttcgagcaaa tttatcttta ctatcaaatt tccataacaa
     1381 agttacaacc gctttatcct ttactcctga atcaagcaat gaagacattt ccttggttat
     1441 aagccgcata atactgtttt taacctcgtc ttcagaaaga ttgtcatcag cagtaataag
     1501 ctcaatactc ggcgagatat tcttcacaac ttcatcaata gatatatcac ccagcctcag
     1561 tcgttcactt aattcagcca gttgcctgta atcggcgtat gtcagctcag actgaacagg
     1621 aaaaagagac acaaaatctt taggtacgga cgctgcttgg agggcacgag tcaccttagc
     1681 tgccgacaat ccctctcttt ccgcaatttg cttctggttc ataccggcct ctttgagacg
     1741 tactaaacgc aacccaattt cacgaatatt atgttcaagt gacgtctgca aatctttcgc
     1801 cagatgctga gcttcagaaa cagtaagctc atcatccgtt acaagtacac gtaatccaac
     1861 cttgcatagc aaagctgctg cccgacgacg ggaaccatcc aaaatctcaa taagatcgcc
     1921 agtccgaatc ccaatacatg gatagaattg ttgtaaacgt atagttctgg tgatgtcttt
     1981 tagtgagtcc ggcgtcaggg ctgtctgttc gcggccattc acctcttcaa ccacaaaagt
     2041 ttgagtatcc actttgtcat gatcaacctt gatctcagta aattttgcct ttcggccagt
     2101 attgagggta aatacatgaa ctggagccga tatttcttca gtattattca ggatatttgt
     2161 attcagcgta cggccaatag tcggtcggtg ctttctgttt tccatcagta gttggccctt
     2221 ataaattcaa tacgatcaaa aactgcttta gcgaaatctt ctgctgccat acgtgcattt
     2281 ttcaaagctt caccactgcc aacataagta acaggattcg cagatatcac ggtatcaaac
     2341 gactcgccgc ttctctcaaa accatcaaga cgtggcattg aaacatcaag catgtcacca
     2401 ccgaaaatct cttttgtcag gctatggcag tatttatgat ctgacttatt cgccagtttc
     2461 gacatgaagc caatatttgc ctgcaaacgg catgaacatc catcctgttc aataatctgt
     2521 acaagttcag gtaaacgagc caaatattta agcgtggaat ggaaatcaac ttgtgctggc
     2581 ggcaccggtg taaacatgat atctgcggca gcaatggcat ttttcaaaaa tgcgtccaga
     2641 tgcgggcctg tatcaataag gatgaagtcg aaatcatgct tcagcttatc aataatattc
     2701 tcacgcaata tcgcgtgttt gttttgtcct aatagatgct cctcgcacag agtatcccag
     2761 tttgatgcaa taaaggcgtc gtcaatagaa gccggcataa cataaacgcc agggataact
     2821 gacggaacga taaaatcttc cagtaattcc tcacgactca cgttctgcaa catagcctgc
     2881 ggtgcggttg tatcaaccag ccccaccgca tgcaagtagt tcagaaacat agtggctgat
     2941 gactggggat ctaaatcaag gagcagaata cgtaaatctt ctgacagcag gtgaggatga
     3001 gcacgcaggg cgtgagctac ggaaacacta ctcacggttt tagacacacc gccttttaga
     3061 ctaccgatga atatggaata tgcctcacta taacgatcgc gatatttagg aatgccacga
     3121 tgtgcataga tatcgataat gttctggatg gttaatgcat attttgtcgc ggttcctgct
     3181 gggcgttttt caaactgata gccctgagcc tccatctcac cgactgcacc atccacgctt
     3241 gcgcgcgtta attttggaag cttagctaca gcagactttg aatagacttg ataaaaaaca
     3301 tcagcctgaa actcctgctt ttgctcctga atctgttttg ttagggcgag caacatttta
     3361 tctgcacgct gcgcaacctt acttaactgc tcaaaactag tcattccccc tccgtgctat
     3421 gtaggatttt aacttattac aaaaaaaact gcattagcag ggtaatgcat gcaagtacat
     3481 attgtacatg cttatattaa aaatgcgcgc attgctgatt tattaatcat cggaatgaat
     3541 attactgtaa gatagtatgc ctttccggga gaaagacgct gatttccccc gaataatcca
     3601 gttcaccacc acactccgga caacagcttt cttcaggctc cagatggtgt atttcgcggg
     3661 aaacatgttc cggtaacgga cggcgatgac gtgactgtcg caacaggcgg gggtagtgct
     3721 tccaatcagt agaacatgtg cttttcaatg aaggagccga tgactttttc gtggatttcc
     3781 acggaacgcg agaagcagat tgttttacgt gccagccgct tgatgcgggt gcggagggtc
     3841 cggttgttac gtgcaatacg ttgcgtgaag attttgccgg tcaggtgttt ctccttcggc
     3901 acttctctgg cgtagctgcc ccagtcatcg ctggtcacca tgccgataca gaacggagtc
     3961 aacagggcta acagttcccg acaggtctca tcattccgtg gcccgaaagt gtaagccaac
     4021 acaccgccag ttttagtgtt gtacgcatac cagagccagt gttgtgtcaa cgacggatga
     4081 aaagtgatcc acttatatct ccaccaacgg cccaatattg atccaccgtt ttactcagga
     4141 ttagcttctg ctataacccc ggcctttcgt ttctgtctga gtcgatagct ttctcctttg
     4201 atttgaacga catgtgagtg gtgtaagata cggcccagca tcgctgaggt cagtgctgca
     4261 tcaccggcga acgtttgatc ccactgcccg aacggtagat tggatgtcag gatcattgcg
     4321 ctcttttcgt aacgtttagc gatgacctgg aagaacagtt ttgcttcttc ctgactgaac
     4381 ggcagatagc ctatttcatc aatgatgagc aggcgggggg ccattactcc acgctgaagc
     4441 gtcgttttat aacggccctg acgttgtgcc gtagataact gaagtaacag atctgctgct
     4501 gtt
//
LOCUS       DAJTRH010000191         4209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTRH010000191 DAJTRH010000000
VERSION     DAJTRH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO339_003954"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO339_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5928376.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO339_003955"
     tRNA            complement(324..399)
                     /locus_tag="OO339_003955"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO339_003956"
     tRNA            complement(406..480)
                     /locus_tag="OO339_003956"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO339_003957"
     tRNA            complement(597..681)
                     /locus_tag="OO339_003957"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO339_003958"
     tRNA            complement(690..765)
                     /locus_tag="OO339_003958"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO339_003959"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO339_003959"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5928377.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO339_003960"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO339_003960"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5928378.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO339_003961"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO339_003961"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5928379.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJTRH010000192         4199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTRH010000192 DAJTRH010000000
VERSION     DAJTRH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            85..753
                     /locus_tag="OO339_003962"
     CDS             85..753
                     /locus_tag="OO339_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928380.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(996..1691)
                     /locus_tag="OO339_003963"
     CDS             complement(996..1691)
                     /locus_tag="OO339_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5928381.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1684..3111)
                     /locus_tag="OO339_003964"
     CDS             complement(1684..3111)
                     /locus_tag="OO339_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5928382.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERITPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3122..3841)
                     /locus_tag="OO339_003965"
     CDS             complement(3122..3841)
                     /locus_tag="OO339_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5928383.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1084 c          869 g         1127 t
ORIGIN      
        1 tttgctgata tttaacccga acattcctga tacagactat taatacaaca ttatctgcga
       61 cagattttat tttctggata tattatgagt gaacaaatca aacaggatat cgatctgatt
      121 gagattttat ttcatctgaa gaaaaagatt cgtgttatcc tttttattat ggctatctgt
      181 atggcaatgg tgctgttgtt tctgtatatc aataaagaca atacaaaagt gatttacagc
      241 cttaaaataa accagacaac gccaggtata cttgttagct gtgatagcaa taataatttt
      301 gcctgtcaga ccacaatgac tgaagatgtt attcagcgaa ttactacatt ttttcagacc
      361 agcccagata tcaagaacag agaaataagg ctggaatggt caggagataa gagagattta
      421 ccaactgctg aagaggaaat atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca
      481 gaataccata atggcaggca agttctcgat gagatacaaa cgccttcagc aattaacagt
      541 gagctttata caaaaatgat atacctgacc aggaactggt cattgtatcc gaacggtgat
      601 ggctgtgtaa ctataagctc accagaaata aaaaataaat accctgctgc catttgcctg
      661 gctctgggat tttttctaag cattgtaatt tctgtaatgt tttgccttgt caaaaaaatg
      721 gtagatgaat accaacaaaa ctctgggcag taataaacag cattctgata gagagtgcct
      781 gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt
      841 ctttttcact gtaaatatcc tggcattact aatgcctgga tatttacaga agagcagcat
      901 aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc
      961 cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat aatcagatac
     1021 accgctttca ccggaccatg aaccccgacg actttgataa gctcaatatc cgccgttgag
     1081 ctgggaccac tgatgatgtt aatacatgac ggcattcgtt caccggcctg cgctttctga
     1141 tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg caggataaaa
     1201 agagaagatt ccgggagcag gctcaatgaa cgcccgcgct cggcggcgga aaaaagaacc
     1261 acgcctcccg attcggttaa accatattca gcatacacga cacccacttt agcctgctct
     1321 gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt gcattcctgc
     1381 tgcaaacgtt cactaatccc caattcctcc agcctcgtgt caccgctaat cacgaccgac
     1441 tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc
     1501 agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc
     1561 tgttggttaa gttgggtaag ccgctcgtta gcatagttgt taagcggcgc atcttctgct
     1621 tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa cgttattcaa aaattcgctc
     1681 cgattatcca ttctttttct cctgcgcctg atgtttctta aaccaactac ggaaactctc
     1741 tccgtcagct tcaggaagat cgcgtgcttc catccagtcg ctaatcgcgc caaatttgag
     1801 tggtgttttg ccgccattga taaaccagct tgccgcatga gcgccggcca tcatcccgac
     1861 tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc gttgctctgc
     1921 ttttgcggtg atcccttttt cagccatcac ccgacgatga cgcaaaatca gttttgacag
     1981 cggaatacgc accggacaca cgctgtcaca agcagtgcat aaagagcagg cgtagggtaa
     2041 atctttaaaa tctttatagc cgccaagtag cggagaaatc accgcaccaa ttggccctgg
     2101 ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag tattcataca
     2161 agccccgcag cgaatacagc gcagcacatc ccgaaattca gaggccagca cctcagaacg
     2221 cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc ccgcttcgcg
     2281 cggccctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac tgcgcgccag
     2341 catggtgatc aatacatcta cctcggcaaa cgtgggggta atacgctcca ttcccatcac
     2401 tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat tggtcaccag
     2461 gcataccgaa ccggtctctg ccaccgcgaa attacagccg gtaataccta tttcagcact
     2521 gaggaaatct tcacggattt tttgccggat gaataaggtc atcgcttcag gcgtttccgg
     2581 cccctcatag cccagacgtt cgtgtagcac tcgacggatc tgatggcgat ctttatgaat
     2641 tgccgggacc acaacatgag atggcggatc ttgatccagc tggagaatat attcacccag
     2701 atcggtttca atcacctgaa tgccagcatc ctgcaacaca tgattgacac caatctcttc
     2761 ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg caacctgtaa
     2821 aatgtagcgg gtagcgtctt ctttggtttt tgcaaaatag acgtgaccgc cgttttgcgt
     2881 cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat gatcacgtat
     2941 ctgggcggcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca ttttttgccg
     3001 atttgcccca atacgctgct gcgcgcttgc caccgctttg cgcatgatcg gatcttcaat
     3061 ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca tctttatatc
     3121 ctcagcggct catcaacact tcagcaatat gcatcacttt gactttctgc ccttcccgtt
     3181 gtaatcgccc actgatgttc agcaggcaac tcacgtcagc accaattaaa tactcagggc
     3241 ggacttccat caggtgcaca accttttctt tcaccatctc gccggatatt tcggccattt
     3301 tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag gtcaacagct
     3361 ccagtccacg cacatttttc agcagcgtaa gtggctcgtc cttcactccc agcttacggg
     3421 ccaggctaca agatgggtga tacacagctc tcccttgcaa actggcacct acatcgacta
     3481 cccctaattt attaacaata aaagaggtga gatcctgcat acgcgcggca accttttcgg
     3541 cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt atggcatagg
     3601 tgcaagagcc agccggtgaa ataatgggat cgtcgttatc ctccagtgcg gcgatcagat
     3661 ttttcatccc tggaatcgct tctttgatat aaccgctatt gatcgcaggc tgaccgcagc
     3721 atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc agcacggagt
     3781 ctcgtgccat tcttgatttc agggcgtcac caatacaggt gacaaagaaa ttgacattca
     3841 caactaatac tccattactt catgcccatt tatgcccgat gtttcccccc caccggggga
     3901 tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc acccctcatt
     3961 tgtatgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat tttatttctt
     4021 tcggcatcgc tcacatttta tggggaaatc gacgatcatt tttggcataa gaaatccatt
     4081 taaaaatggc tacatccctt atacctattc agagctactt attcaggggt tattgagata
     4141 tacactgatg gtgtacaaaa aaatcaccga caataatcac gtatcaagta atagtcgga
//
LOCUS       DAJTRH010000193         4104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTRH010000193 DAJTRH010000000
VERSION     DAJTRH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..148)
                     /locus_tag="OO339_003966"
     CDS             complement(<1..148)
                     /locus_tag="OO339_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR5928384.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGL"
     gene            complement(298..459)
                     /locus_tag="OO339_003967"
     CDS             complement(298..459)
                     /locus_tag="OO339_003967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928385.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(456..644)
                     /locus_tag="OO339_003968"
     CDS             complement(456..644)
                     /locus_tag="OO339_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5928386.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            904..1239
                     /gene="iraM"
                     /locus_tag="OO339_003969"
     CDS             904..1239
                     /gene="iraM"
                     /locus_tag="OO339_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5928387.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1520..1628)
                     /locus_tag="OO339_003970"
                     /pseudo
     CDS             complement(1520..1628)
                     /locus_tag="OO339_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2151..2227)
                     /locus_tag="OO339_003971"
     tRNA            complement(2151..2227)
                     /locus_tag="OO339_003971"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2191..2193),aa:Arg,seq:tct)
     gene            complement(2329..2404)
                     /locus_tag="OO339_003972"
     tRNA            complement(2329..2404)
                     /locus_tag="OO339_003972"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2369..2371),aa:Ile,seq:cat)
     gene            complement(2549..3370)
                     /locus_tag="OO339_003973"
     CDS             complement(2549..3370)
                     /locus_tag="OO339_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5928388.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3385..3741)
                     /locus_tag="OO339_003974"
     CDS             complement(3385..3741)
                     /locus_tag="OO339_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5928389.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3754..>4104)
                     /locus_tag="OO339_003975"
     CDS             complement(3754..>4104)
                     /locus_tag="OO339_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5928390.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1151 a         1116 c          792 g         1045 t
ORIGIN      
        1 gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac ggggaaaccc
       61 tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg tatgacacac
      121 ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga agctgacaac
      181 aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag cgtctcagac
      241 gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc tcacttttta
      301 tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc catacgttgc
      361 atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac tgtcttttat
      421 ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt tgcaccactg
      481 accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt tatggccacc
      541 ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc cgcaagatct
      601 gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct ttccaacatc
      661 tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa aaccatacct
      721 taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac gagatataaa
      781 ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt tctatggttt
      841 aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa acaaggaaac
      901 taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat atctttttca
      961 gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt cttacctcct
     1021 gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt gctacctgta
     1081 accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa aaacagccag
     1141 gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg ttgcactctg
     1201 caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc acatctgaca
     1261 aaatcaatat aaacagcccc tcgggagagg ggctggagag tggcgctatg tgccattcca
     1321 tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga ttatcccata
     1381 tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt tcttttaaca
     1441 aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt aaaaatttct
     1501 cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac gggaaagcaa
     1561 caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc cggctgctgc
     1621 aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga taatccagat
     1681 atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc tggctattct
     1741 gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact catcgcaaac
     1801 cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac gatcaggagt
     1861 acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga ctatatcaat
     1921 acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac ccacaccata
     1981 tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat actcaatcaa
     2041 tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata tcctgagcca
     2101 ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata tggcgcgctg
     2161 tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc ggctgagcta
     2221 acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca ccaacaaccc
     2281 tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg gtggcccttg
     2341 ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag
     2401 ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa cccgctcagc
     2461 ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta tgacgaattt
     2521 accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag
     2581 cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc ttaactgttt
     2641 tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta aatgcctccg
     2701 ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg ataacaggca
     2761 taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg caatccttac
     2821 aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt accacctccc
     2881 tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac tttgcccata
     2941 ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt tcaataaatt
     3001 tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca taatcctcaa
     3061 aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga agttttctca
     3121 acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct gttgcccgcg
     3181 ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc atctcagccc
     3241 gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca tcttttgacg
     3301 ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc ggtaaatctt
     3361 ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca cccccagacg
     3421 tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct catcgtccat
     3481 taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt ccaggtcgcg
     3541 acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg acaggcttaa
     3601 tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct ttccctcctc
     3661 cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg gcgggtaagg
     3721 taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag ttgcaaaggc
     3781 gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt cgaacgccag
     3841 aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga aaatatcgtg
     3901 agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat cgtcggctgg
     3961 cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct cgttaaccca
     4021 gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa cggcaatgct
     4081 gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAJTRH010000194         4042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTRH010000194 DAJTRH010000000
VERSION     DAJTRH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(886..1077)
                     /locus_tag="OO339_003976"
     CDS             complement(886..1077)
                     /locus_tag="OO339_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5928391.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="OO339_003977"
     CDS             1142..1465
                     /locus_tag="OO339_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5928392.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO339_003978"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO339_003978"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO339_003979"
     CDS             2143..2673
                     /locus_tag="OO339_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5928393.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO339_003980"
     CDS             2680..2913
                     /locus_tag="OO339_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5928394.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="OO339_003981"
     CDS             complement(3459..3851)
                     /locus_tag="OO339_003981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928395.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTRH010000195         4006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTRH010000195 DAJTRH010000000
VERSION     DAJTRH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO339_003982"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO339_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5928396.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1472..>4006
                     /gene="adhE"
                     /locus_tag="OO339_003983"
                     /gene_synonym="adhC"
     CDS             1472..>4006
                     /gene="adhE"
                     /locus_tag="OO339_003983"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5928397.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGAN"
BASE COUNT         1112 a          977 c          919 g          998 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
     3961 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaac
//
LOCUS       DAJTRH010000196         3961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTRH010000196 DAJTRH010000000
VERSION     DAJTRH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..224
                     /locus_tag="OO339_003984"
     CDS             <1..224
                     /locus_tag="OO339_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5928398.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OO339_003985"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OO339_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5928399.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OO339_003986"
     CDS             518..1108
                     /locus_tag="OO339_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5928400.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OO339_003987"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OO339_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5928401.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OO339_003988"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OO339_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5928402.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OO339_003989"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OO339_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5928403.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="OO339_003990"
     CDS             3457..3648
                     /locus_tag="OO339_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928404.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1071 a          982 c          986 g          922 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctccatat gaacggaacc
     3781 gacgattaaa ctggatggct ctgattgatt cagggtatga atggcggttt tttgctccgt
     3841 ttccctcaaa atggacgcaa cttcccctct gcggctctca gccgcaccac cgcatccggg
     3901 ccagcggctc atgcatcagg acctgctctg ccagacggta gccccgcttc agccccgtaa
     3961 a
//
LOCUS       DAJTRH010000197         3758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTRH010000197 DAJTRH010000000
VERSION     DAJTRH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            108..2105
                     /gene="betT"
                     /locus_tag="OO339_003991"
     CDS             108..2105
                     /gene="betT"
                     /locus_tag="OO339_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR5928405.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="OO339_003992"
     CDS             2168..3445
                     /locus_tag="OO339_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5928406.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          908 a          855 c          945 g         1050 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaagc gaggttcttg ttttcaaatt gttccgga
//
LOCUS       DAJTRH010000198         3646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTRH010000198 DAJTRH010000000
VERSION     DAJTRH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            28..315
                     /gene="relE"
                     /locus_tag="OO339_003993"
     CDS             28..315
                     /gene="relE"
                     /locus_tag="OO339_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5928407.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            387..542
                     /gene="hokD"
                     /locus_tag="OO339_003994"
     CDS             387..542
                     /gene="hokD"
                     /locus_tag="OO339_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5928408.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            760..1011
                     /locus_tag="OO339_003995"
     CDS             760..1011
                     /locus_tag="OO339_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928409.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1078..1356
                     /locus_tag="OO339_003996"
                     /pseudo
     CDS             1078..1356
                     /locus_tag="OO339_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1358..2416
                     /locus_tag="OO339_003997"
     CDS             1358..2416
                     /locus_tag="OO339_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5928410.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2417..2782
                     /locus_tag="OO339_003998"
     CDS             2417..2782
                     /locus_tag="OO339_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5928411.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            2779..3462
                     /locus_tag="OO339_003999"
     CDS             2779..3462
                     /locus_tag="OO339_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5928412.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          969 a          823 c          985 g          869 t
ORIGIN      
        1 agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga cgagcgggca
       61 ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa gaagctggtt
      121 gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc tgattgttac
      181 aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga cgagaaagtt
      241 gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag cgaggcggtc
      301 aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt gacacttctg
      361 ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgtta atcgccctga
      421 tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac ctctgcgagg
      481 tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa cctgaggagt
      541 aagagacccg gcgagtgaga aatccctcgc cacctctgat gtggcaggca tcctcaacgc
      601 acccgcactt aacccgcttc ggcggttttt tgtttttatt ttcaacgcat ttgaagttct
      661 ggatggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa aagggatgga
      721 ccccgaacag gggagtgcta cttatctgga aggattctga tgatgaacat cgaagaactg
      781 cgtaaaattt tttgtgaaga tggcctctat gctgtgtgcg ttgaaaatgg aaatattgtt
      841 agtcattacc gcattgtgtg tttgcaaaaa aatggggctg cgttaattaa ttttgtggat
      901 gcccgagtga cggacggatt tatcttgcgc gacggtgagt ttgtcacttc attacaggca
      961 ttgaaagaga tcggaataaa agctggcttt tctgcttttt cagaagaata aactcatcta
     1021 caatcttgcg cggggctgaa ctcccgctga gtaacaccgt gccaccggag aaaaccgatg
     1081 gcacgcaacg caaaatatta caatcatgat aattcgaccg ttcttgccca cacgcacgag
     1141 cggtattctc acgcatttaa gtcagactgg taccagcatc cctcatgcac tgaagaacag
     1201 gccgaatggc ttattcagtg ttaccgcagg cgcggatgcg aggttaaaaa agcccttagc
     1261 ctcgactacc gtcactagat aatctccgtc aggctccctt actccgaaca gccaccgcgt
     1321 ccgtcccgca cattccagca acggatctgg aggtaacgtg cgggtattac ttcgacctgt
     1381 tctggttccg gaactcgggc tggtggtcct taagcccggt cgtgaatcca tgccggtatt
     1441 ccacaatacc cgggtactgg tggagccgga accgaaaagc atgcgtaatc tgccgtccgg
     1501 ggtcgttcct gccgttcgcc agccgctagt ggaagacaaa acattgctgc cgtttttcag
     1561 taacgcacgg gtgattcgtg ctgctggtgg tgctggcgca ttgtctgact ggctgttgcg
     1621 ccatattaaa tcctgccagt ggccacacgg cgattatcat cacagcgaaa ccgtcattca
     1681 ccgttatggt accggcgcaa tggtattgtg ctggcactgc gacaaccagc tgcgtgacca
     1741 gacatccgaa tcactcgagc aacttgctca tcaaaacctg tcagcatgga tgattgacgt
     1801 catcggtcac gcaataagcg gtacgcagga gcgtgaatta tctctggctg aattatcctg
     1861 gtgggcggtc cgcaatcagg tggcggacgc gctaccggaa gcggtattac gtcgttcgct
     1921 ggggttgcgt gcggaaaaaa tccgctcaat gtaccgtgaa agcgacatcg tgccgggaga
     1981 gcagaccgcc accagcatac tgaagcagcg cacaaaaaat cttgcgccgc tgcctcacgc
     2041 ccaccagcaa cagaatccac cacaggaaga gacggtggtc agcattgccg ttgatccgga
     2101 atctccggct cagtatctcc agcgccagaa accacaacgg gaagagatgc ctgtatacac
     2161 gcgctgggta aaaacgcaga aatgcatgac gtgtggcaat caggcagatg atccgcacca
     2221 catcattggt catggactgg gagggatggg aacaaaggct gacgatttgt ttgttattcc
     2281 gctgtgtcgt aaatgccata acgaactaca cgccggggta aaagattttg aagaaaaaca
     2341 cggtagtcag ttgttgttgc tgattcgttt tttaatgcac gcgagaaatt cgggtgttct
     2401 gaagtggaaa gcataaatga ccgaacgcat agaatttgtt ttgccttacc cgccaacggt
     2461 gaacacctac tggcgacgtc gtggcagcac atattttgta tcaaaagccg gtgagcgtta
     2521 tcgccgtgat gtggcgctaa ttgttcgcca gcagcgactg aaattaaacc tgtccggaag
     2581 gctggcgata aagattattg cagagccacc ggataagcgc cgtcgtgacc tggacaatat
     2641 cctgaaggca ccgctggatg cgctgacgca tgctggacta cttatagacg acgagcagtt
     2701 tgatgaaatc aatattgtgc gcggtcagct cgttcctggt gggcggctgg ggataaaaat
     2761 cacagaactg gggtgcgcat gaataaccag tatttacagt ttgtgcgtga gcagctcatt
     2821 atcgccaccg ctgatttgag tggggcaaca aaaggtcagc ttgaagcctg gcaagagaat
     2881 gccatgttcg atacagggcg ttacaggcgt aaaaaaatcc ggtaccgcga tgaagtgact
     2941 ggaaaaatga taacgcggga taatccacca atcccgggaa agcaatcgct ggcgaagggg
     3001 acgtcaattc ctctggtcag tccggttgag ttttcgacat catcgtggcg gcgggctgtt
     3061 ctgtctcttg aagagcacta taaagcctgg ttgttgtggt gttacagcgg gagtatttgt
     3121 tgggaatatc agatcgcgat aacacagtgg gcgtggaatg aatttaatgc tcaatccggt
     3181 accagaaaaa ttgcagggaa aacgcaggaa cgcctgaaaa aattaatctg gctggcggcg
     3241 caggcagtaa aagcagaact ttttggtggg gaaggttatg aataccagga gctggcatta
     3301 ctggcgggag tgacaactaa aaactggtcc aaaacattta ctcgtcactg ggttgcaatg
     3361 aaacacattt ttcaccgact ggatagtgag gctttattgt ttgtaatgag aacacgttca
     3421 aaaaaaagac ggcattttca aagcaaagtg ttgcaaaagt agattgaaag gcatatattc
     3481 atgcaaatct gatactttgc cgattttgta cgtgatggca aaagcaaaca aaacccgccc
     3541 acaagcgggt tttttgtgcc acttatctcg gatagacatg gtgaatgcgc tggtggagga
     3601 gataagggtg atttttgaat gcttgcaaca ttgatttcgt aacgtt
//
LOCUS       DAJTRH010000199         3499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTRH010000199 DAJTRH010000000
VERSION     DAJTRH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..66)
                     /locus_tag="OO339_004000"
     CDS             complement(<1..66)
                     /locus_tag="OO339_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5928413.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMW"
     gene            complement(66..2143)
                     /gene="flhA"
                     /locus_tag="OO339_004001"
                     /pseudo
     CDS             complement(66..2143)
                     /gene="flhA"
                     /locus_tag="OO339_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2136..3283)
                     /gene="flhB"
                     /locus_tag="OO339_004002"
                     /pseudo
     CDS             complement(2136..3283)
                     /gene="flhB"
                     /locus_tag="OO339_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          852 a         1030 c          860 g          757 t
ORIGIN      
        1 ccacatcccc tccccggcgg cttgcaccag cagcggaaac aataatatcg ctaataaggt
       61 tctcatcatt taccgccaat tgtcgccgtc atgcggatat gtcggttatc agacagttcc
      121 agattcgaca ggaccactaa ctgcggcaag ctgcggcgca ggaagcgaga caataatggt
      181 cgcagcgcgt ggttcaccaa caatactggc ggcgcaccca gcatctcctg acgggataga
      241 gcttcctgag tttgcgccag taaacgatcc gccagccctg gctccagtcc tcccccgccc
      301 tgcagcgcct gtagtaacaa acgttccagc ggtgtatcga ggccaataac atggacttca
      361 tctttgccag gaaaccactg ctgggtaatc gcccgtccca acgccacgcg cacgacggcg
      421 cttaattcat gtggatcgct ttggatgggc gcatgttccg ccagcgtttc gagaatggtg
      481 cgcatatcgc gaatcggtac tttttcatcg aggagatttt gcagcacttt atgcagtgtg
      541 gtgagcgtga cgacgccagg aacgagatct tccgtcagct ttggcatctc ctgggcgacg
      601 cgatccaaca gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg ctggctaatg
      661 aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta cccctgaatc
      721 tgcgcctgtt cttttagcgc actttcaatc cagatagcat tcaggccaaa tgccggatcg
      781 acggtcgcct caccaggtaa cgtcccggca gcggttccag ggttaatcgc cagccagcgc
      841 cccggataag catcaccact gccaatctcc acgcctttca tcaaaatgcg atagcgggca
      901 ggttgcagat ccatattgtc gcgaatgtgc accactggcg gcagaaatcc catctcctgg
      961 gcaaatttct tgcggatact gcgtatacgg cccaacaact caccatcctg ctggaaatcg
     1021 accatcggga tcagtcgata acccacttcc attcccagag aatcttccag ttgtacatcg
     1081 ttccacgtcg cttcgacaac ggtattattc tctgccattt ttaccggttt gggttcggca
     1141 ggcgcttttt gttcgcgtcc gcgtatccac caggccagcc ccagcaatcc ggcagtgaac
     1201 agcaaaaata ccaggttcgg cattccaggc accaggccga gtaaaccgag cacggcggcg
     1261 cttaacaaca taacgcttgg gttactgaaa agctgattca ccatctgctc gccaacatcc
     1321 tgatcggtgc tgacacgcgt aacgatgccc cccgcggcgg tagaaatcac cagcgccgga
     1381 atttgtgcca ccagaccgtc gccaatggtc aatagcgtat aactttccgc cgcgtgtccc
     1441 atgctcatgc cagttgcagc acgccgacca gcaacccgcc gacaacgtta atgaccatga
     1501 tgaggatccc ggcaatggca tcgccgcgaa caaacttact tgccccgtcc attgagccgt
     1561 aaaaatcggc ttcctgagtc acttcggagc ggcgtttttt cgcctcatct tcaccaatca
     1621 atccagcgtt aaggtcggcg tcaatcgcca tctgcttacc cggcatacca tcgagaacaa
     1681 agcgcgcacc cacttctgct atacgcccgg cacctttggt aatgaccata aagttgatga
     1741 tcacgagaat gacaaacacc acgataccga tagcgaaatt gccaccaacg aggaagtgac
     1801 cgaacgcttc gaccaccttc ccagccgccg ccgcgccggt atgcccttcc atcaaaatga
     1861 tacgggttga agccacgtta agtgccagac gcaacaacat agtaaacaac agaatggtcg
     1921 gaaacgcagc aaactcaagc gtgcgctggg taaacatcgc caccagcaac accatgatcg
     1981 acaaggcaat attgaaggta aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca
     2041 tcatcgacaa gatcaacagg atcaaaatcg gtccggcaag gatctgccat tatgtcgatt
     2101 tcaggtttgc gggcaggcgc agcatcgcgg ccagattact catgggtcgg tttctcgtta
     2161 ataaaatcca gggctgccgg caccggaaga tgagtaggtg tacagggcgc tgtccaccag
     2221 ccagacgcca gcgtttcagt tgccagaccc aggccagcac ttccgccacc gcggcgtaca
     2281 gttgacccgg gatttgttga ccaatctccg catgtcgata cagcgctcgc gccagcggcg
     2341 gcgcttcaag cgtcgggacg ttattttcag cgccaatttc acgaatgcgc agcgcgacca
     2401 gccctgcacc tttagcgacc actttcggtg cgctcatttt gttttcgtca tactgcaacg
     2461 ctaccgaata gtgggtcggg ttattgacaa tgacatccgc tttcggcaca tcggccatca
     2521 tccgacgccg tgcggcagct cgctgcatct gacagatccg tcctttaaca tgggggtcgc
     2581 cttcgctttg tttgaactca tcacgaatat cctgccgcga cattcgcagc tttttcaggt
     2641 ggctgaagat ttggaaaaag acgtcaaatc ccaccattgg aatgacacca agcaccacca
     2701 gcagtgcgca tagccctacc aaatccatcg cattacccat ggcggtaatc ggtgactcgg
     2761 ccattaagcg catcatctgc ggccagtgat gccagagaaa aaaccccgtc acgctgccaa
     2821 ccaggatggt tttcaaaatt gccttaagca actccgcgct agtctgtgcc gagaatatcc
     2881 gtttaatgcc cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa
     2941 ataccagccc ccccagcata accggagaaa tgagcgccac cagcaccacg ccgctaatca
     3001 gcggtagcag cgccagcatg gcttctctga tcagcagaat aatttgcccg aggatcagat
     3061 tcgggtcttt gataatactg tgatcaaaat gcagcccagc ggagagcatg cccgacaatc
     3121 gacgggccag cgacacaccg ccaaaccaga taacactaac gcccaccagc aaaatcagca
     3181 gtgaggtcag ttcacgggaa cgcgggattt gcccctcttc ccgcgctttt tctagtcggt
     3241 gaggtgtggg ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa
     3301 gagtattaag cgtaaatgat gccagagcgc aaagcgttca atggtttaag taaggggcaa
     3361 aacaggcggg atttagggcg tttatgcaaa aataggttca gttgcagaac gttttgcgaa
     3421 ccattttcca gttctgatat ttagccattt agcctataga atcgcactcc gcctctctgt
     3481 tagtttgcta ataattaaa
//
LOCUS       DAJTRH010000200         3458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTRH010000200 DAJTRH010000000
VERSION     DAJTRH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(60..590)
                     /gene="cybB"
                     /locus_tag="OO339_004003"
     CDS             complement(60..590)
                     /gene="cybB"
                     /locus_tag="OO339_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5928414.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            779..1779
                     /gene="gap"
                     /locus_tag="OO339_004004"
                     /pseudo
     CDS             779..1779
                     /gene="gap"
                     /locus_tag="OO339_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            complement(1821..3260)
                     /gene="aldA"
                     /locus_tag="OO339_004005"
     CDS             complement(1821..3260)
                     /gene="aldA"
                     /locus_tag="OO339_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5928415.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
BASE COUNT          916 a          882 c          788 g          872 t
ORIGIN      
        1 cgtattcttc gcctgtacca cgggtcggtt ttagtacagg cgttttcttt aaatatcctt
       61 caggaacgtt tacgcggcat catgcgtaga agtgtgttgt ccttccagaa atagtggtgt
      121 gccagtgcag ccgcagcgtg caacccgatg acaaaatatc ccagattcgc cagcgtctca
      181 tgccacgact ttaagctatc aacccgttcg aaattggcct ctgaagcgta aggcatcgtc
      241 aaaccaaacg caaaccacgg gttgccccgg ttatacatca tcaccaaacc aatcactggc
      301 agcgcaataa acaggagata aatcaccaaa tgtcccaaat gcgccagtcc cgtcatcatc
      361 ggtttcggct taggtataat cggcggggtt gggtatttca gtcttaacaa aagacgaacc
      421 accatcagca cgagaattga gatgccacag gaaacatgaa tcatgttgat gagcggccga
      481 tcgctacgtg ggaagaaacc acgaaactcc attgcgcaat acgctgcgat aaccagcaaa
      541 aagaccagcc agtgaatgct gatttgtaac cttgagtatt tattttccat aacatttcct
      601 gctttaacat aattttccgt taacataacg ggcttttctc aaaatttcat taaatattgt
      661 tcacccgttt tcagtacgtt acgtttcact gtaagaatgg agattgccca tcccacaatc
      721 ctggtctaag cctggaaagg atcaattttc atccgaacgt tcctgacagg agaaaaccat
      781 gagtaaagtt ggtattaacg gttttggtcg tatcggtcga ctggtgttgc gtcgtttact
      841 tgaagtcaaa agcaacatag acgttgtcgc tattaatgat cttacttccc caaaaattct
      901 cgcctatctg ctgaaacatg attcaaacta tggtccgttc ccctggagcg ttgattttac
      961 ggaagattca cttatcgttg atgggaaaag tatcgcggtt tacgccgaaa aagaggctaa
     1021 aaatattccg tggaaagcga aaggggcaga aatcattgtc gaatgtactg gcttttatac
     1081 ctccgccgat aaatcgcagg cgcatcttga tgctggtgcg aagaaggtgt tgatttccgc
     1141 tcctgccggt gaaatgaaaa ccatcgttta taacgttaat gacgacactc tggatggcaa
     1201 cgacaccatt gtttccgtgg cgtcatgcac cactaactgc cttgcgccga tggccaaagc
     1261 cttgcatgac agtttcggga tagaagtcgg cacgatgacg accattcatg cctatactgg
     1321 cacccagtca ctggtggatg gcccgcgtgg taaagattta cgtgcttcac gcgcagcggc
     1381 agaaaatatc attccccaca ctacgggggc ggcaaaagcc attggtctgg tgatcccgga
     1441 actgagcggc aaactgaaag gtcatgcgca acgcgtgccg gtgaaaacag gttcggtcac
     1501 tgagctggtg tccattctcg gaaaaaagtg actgccgaag aggtgaataa cgcacttaaa
     1561 caagcaacca ccaataacga gtcatttggt tataccgatg aagaaatagt ctcttccgat
     1621 atcattggca gccatttcgg ttcagtgttt gatgccacgc aaacggaaat taccgccgtg
     1681 ggcgatttac aactggtgaa aacggtcgcc tggtacgata acgaatatgg cttcgtcacg
     1741 cagcttattc gcaccctcga aaaattcgct aaactctgac gcgcacaggc ggaggaaaaa
     1801 acctccgcct ctttcactca ttaagactgt aaataaacca cctgggtctg cagatattca
     1861 tgcaggccat gtttaccatc tgcgccgcca ataccggatt tacgccatcc ggcgtggaaa
     1921 ccttgcatag cttcgaagtt ttcacggttg atgtaagttt caccaaactt cagcccttta
     1981 atggctttca tcgcgacgtt cagattttgg gtatagattg atgaggtcag gccgtaatca
     2041 ctgtcattag ccattgagat agcatcttcc agcgtgtcaa atgcgacaac tggcagcacc
     2101 gggccaaagg tttcctcatg cataatcgac atttcctggc gaacatccag cagcaatgtc
     2161 ggcggataat aatatccttt cccctctacc gctttgccac ccaacgccac tctcgcccct
     2221 tcttctactg cgcgcgccac tttttgctca accctttcca gcgccgcggc gttaatcaac
     2281 ggccccatcg caatgtcgtt gcgttcagcg gggttaccaa attgaaccgc ctgcaacgct
     2341 tcacccagca gattgacgaa ctgatcataa atgcctttct gtacataaac acgttctgca
     2401 cagttacaca cttgcccact attaatgacg cgtgaatcaa cgatggcttt gactgccagt
     2461 tcaagatcgg catcgtccat tacgatagcc ggtgctttac cacccaattc cagacacact
     2521 ttggtgatgt ttttcgccgc agtcgccatg atcttctcac ctgcagagac gctgcctgtc
     2581 atactgacca ttgcgacctt tgggttaccc gccagttctt gcccaacggt ttcaccacgc
     2641 cccagtacaa ggttaaacac gccgcgcgga aggcctattt catcgacgat tttggcgaat
     2701 gcaatcgcat tgtttggcgt aaattcactg ggtttaatga cgatggtatt accggtcaaa
     2761 agagcgggag ccattttgcg ggcaatgagg aagaacggaa agttccacgg cagaatgccg
     2821 gtagtcacac caagcgcacg tttaaacaga agaatatttt ctcccggacg atcgctttga
     2881 ataatctcgc cctcgtaacg ccgtgcccac tccgccatgt aatcgatata gtcggcagta
     2941 aaagccactt cgacttcagc tagctgctgg atcttgcccc cttcttcaac aatcagcgca
     3001 ctgatttcac tggcgcgttc gcggatcccg gcggagattt tgcacaacca actggcgcgt
     3061 tcaatagcag gcaacgcttc ccattctggt tgtgcacgtt ctgctgcatc gattgcctta
     3121 cgggcatcct cggcctgacc atcgggtatg cgggaaatga cagcctctgt agcagggttt
     3181 accacatcaa tccatgcgtc tccacgccag gtaacaaact gtccatcgat atacatagga
     3241 tgttgaacgg gtactgacat gggcgactct tgtgatttat atgtttgttt tcggtgaata
     3301 tattgttaat taacatcaat catcagagag acggcatcgg gaaaggacaa gtctgtggag
     3361 ggcttcataa aaacaagtgt aaaaggaata aaaggcagaa ataaaagtca ggagtcatgc
     3421 ccgtttgcaa acaagtggtt cacaaacggg caattttt
//
LOCUS       DAJTRH010000201         3414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTRH010000201 DAJTRH010000000
VERSION     DAJTRH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..238
                     /locus_tag="OO339_004006"
     CDS             <1..238
                     /locus_tag="OO339_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5928416.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OO339_004007"
     CDS             248..886
                     /locus_tag="OO339_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5928417.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OO339_004008"
     CDS             889..2052
                     /locus_tag="OO339_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5928418.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3414
                     /locus_tag="OO339_004009"
     CDS             2136..>3414
                     /locus_tag="OO339_004009"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5928419.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggtt
//
LOCUS       DAJTRH010000202         3409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTRH010000202 DAJTRH010000000
VERSION     DAJTRH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..1927)
                     /gene="metG"
                     /locus_tag="OO339_004010"
     CDS             complement(<1..1927)
                     /gene="metG"
                     /locus_tag="OO339_004010"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5928420.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKM"
     gene            2059..3168
                     /gene="apbC"
                     /locus_tag="OO339_004011"
     CDS             2059..3168
                     /gene="apbC"
                     /locus_tag="OO339_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5928421.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          811 a          860 c          902 g          836 t
ORIGIN      
        1 gcattttacg tggtgccagg ttagccacca taatggtgtg acgaccaatc agtgcctgcg
       61 gatccgggta agcagaacga ataccggaga agacattgcg tttttcaccg ccgagatcca
      121 gcgtcaggcg cagcagtttg tcagaacctt caacaaactc tgcgttttcg atcagcgcca
      181 cgcgcaggtc aactttggcg aagtcgtcaa aggtgatggt ttcctgaatc ggatcatctg
      241 ccagtgggcc agtaactggc gcggtagtgg ctttcacttc ttctttagag gcttccacca
      301 gagcttcaac ctgtttcata tcgatgcggt tatacagcgc cttgaacgga ttcactttgt
      361 ggcccagcag cggttgctgg ataccatccc aggtcagttc cgtattgagg aaggcttctg
      421 cacgctcggt cagtttcggc agtacaggct tcaggtaagt catcagtacg cggaacaggt
      481 tgatgcccat tgagcaaatc gcctgcaggt cagcatcgcg accttcctgt tttgcgacta
      541 cccacggagc ctgctcgtcg acatagcggt tagccaggtc agccagtgcc atgatttcgc
      601 gcactgcttt accaaattca cggctttccc acgcttcacc aatcacttca gcggcatcgg
      661 taaaggtttt gtacagctca gggtcagcca gctcgcttgc cagcacaccg tcaaaacgct
      721 tgttgataaa gcccgcatta cgggaggcca ggttaaccac tttgttcacg atatcggcat
      781 tcacacgctg aacgaaatct tccaggttga gatcgatatc atcaatgcgc gaagagagtt
      841 tcgcagtgta gtagtaacgc aggctgtcag cgtcaaaatg attcagccag gtgctggctt
      901 taataaaggt gccgcgagac ttggacatct tcgcgccgtt caccgtcaca tagccatgaa
      961 caaacaggtt ggtcggcttg cggaagttgc tgccttccag catggcaggc cagaacaggc
     1021 tgtggaagta aacgatatct ttaccgatga agtggtacag ctcggcggtg gagtctttct
     1081 tccagtattc atcgaagctt acgctgtcgc cgcgcttgtc gcacagattc ttgaaagaac
     1141 ccatgtagcc aatcggtgcg tccagccaga cgtagaaata tttgcccggt gcgttcggaa
     1201 tttcaaaacc aaagtatggc gcgtcgcggg agatatccca ctgttgcagg ccggattcaa
     1261 accactcctg cattttgttt gccacctgct cctgcaacgc accgctgcgg gtccatgcct
     1321 gcagcatttc gctgaaagag ggcagatcaa agaagaagtg ttcagaatca cgcatcactg
     1381 gtgtagcacc agaaaccacc gatttcggct cgatcaattc agtcgggctg taggtcgcgc
     1441 cgcagacttc gcagttatcg ccgtattgat ccggggattt acatttcggg caggtgcctt
     1501 tcacaaaacg gtccggcagg aacatgcctt tttccggatc gtacagctga gagatggtgc
     1561 ggtttttaat aaaaccgttt tctttcaggc gagagtagat aagttctgac aactggcggt
     1621 tctcttcgct gtgcgtcgag tgatagttgt catagctgat gttaaagcct gcgaaatcag
     1681 tctgatgctc ctgactcatt tcgccaatca tctgctccgg ggtgataccg agctgctgag
     1741 ctttcagcat gatcggtgta ccgtgggcat cgtcggcgca gatgaagttg acctcgtggc
     1801 cgcgcattcg ctggtaacgg acccagacat cagcctggat gtgctccagc atatggccga
     1861 ggtggattga gccgttagcg tacggcagtg cgcacgtcac cagaattttc ttcgcgactt
     1921 gagtcatagt aggcattact tcttaatagt gaaaaggggt tagatagtac caaatgggaa
     1981 aatgttaagt aagtacttct ctatattctt caggtaaact atcagctgat ggtttatcta
     2041 tacaaaaaag gagtcgggat gaacgaacaa tcccaggcca aatcaccaga agccttgcgc
     2101 gcaatggtcg ccgggacact ggccaatttt cagcacccaa ccctgaagca taacctcacc
     2161 acgctgaaag cgttgcacca tgtcgcctgg atggacgaca cgttgcatgt ggaactggtt
     2221 atgccgtttg tctggcatag cgcgttcgaa gagttaaaag agcaatgtag cgccgaactg
     2281 ctgcgcatca ccggggcgaa agctatcgac tggaaactgt cgcataacat agccactctg
     2341 aaacgcgtga agaatcagcc gggaattaac ggtgtgaaga acattatcgc ggtgagttcc
     2401 ggtaaaggcg gcgtgggtaa atcgtccacg gcggtaaacc tggcgctggc actggcggcg
     2461 gaaggggcga aagtcggcat tctggacgcc gatatctacg gcccatcaat tcccaccatg
     2521 ctgggcgcag aaaatcagcg tccaacttcg ccggacggta cccacatggc accgattatg
     2581 tctcatggcc tggcaaccaa ttcaattggt tatctggtca ctgacgacaa tgcgatggtg
     2641 tggcgtggac cgatggcggg caaggcgtta atgcagatgt tgcaggaaac cttgtggccg
     2701 gatctcgact atctggtgct tgatatgccg ccaggcaccg gtgacattca attgacgctg
     2761 gcgcaaaaca ttccagtaac tggtgcagtc gtggtcacca ccccgcagga catcgcgttg
     2821 atcgatgcga aaaaaggcat tgtgatgttc gagaaagtcg aggttccggt gctcggtatt
     2881 gtcgagaaca tgagtgtgca tatttgcagt aactgtggtc accatgaacc gattttcggt
     2941 accggtggtg cagagaaact ggctgaaaaa taccatacac agctgctggg gcagatgccg
     3001 ttacacatct ccttacgtga ggatctcgat aaaggcacgc caacggttat cagccgtccg
     3061 gagagcgaat ttaccgctat ctaccgccag ctggctgacc gcgttgcagc tcagctctac
     3121 tggcagggtg aagtcattcc tggcgagatt tccttccgcg cggtctaacg cctattaacc
     3181 attctggtta ttcttcatac accgcagaga tgttatattg atgcggtgta tttgtttttg
     3241 ccgcattaat gaacacttat ttaatactgc gaatattctg cggaccttcg tccgaaactc
     3301 tacaaagtcg agtgatcgta caggcaatgc ttcattatca tccttcgtgc tgtgtagtgg
     3361 atgtacagct atagtttgtt atgcactcat tctatccagg cataacctt
//
LOCUS       DAJTRH010000203         3323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTRH010000203 DAJTRH010000000
VERSION     DAJTRH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..113)
                     /locus_tag="OO339_004012"
     CDS             complement(<1..113)
                     /locus_tag="OO339_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5928422.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVK"
     gene            complement(110..2749)
                     /locus_tag="OO339_004013"
     CDS             complement(110..2749)
                     /locus_tag="OO339_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5928423.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            2957..>3323
                     /gene="ldhA"
                     /locus_tag="OO339_004014"
     CDS             2957..>3323
                     /gene="ldhA"
                     /locus_tag="OO339_004014"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5928424.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHR"
BASE COUNT          835 a          887 c          782 g          819 t
ORIGIN      
        1 attttaacgt tcatattgat agtgatcggt tccttaggtg cagcgacttc aatgcgaggc
       61 gtacagccaa ccagcataaa agatgacgtc aacgcagcca gtaaaatttt cattgttttt
      121 cctcacactc ttttccttgc ggacagtcat ttcccggcag acgtgcgttc tgctccaacc
      181 atgcctggag attatcgcca aagcgtaaac tgcgccacag cgtaaaaata ttctcttcat
      241 gatggtaatt aaggtttacc gtaccgcttt taccatcaac gcgactggta cctgtaatgt
      301 tggcctgcat ggttaataaa ccgagattat ctaagttaat ttttgtcgat gaccggctaa
      361 tttccatata gcgcaaccag ttaattgctg aacccgcagt catattgtct ttcaccaccg
      421 catctgcggt atctttatcc agccgcaagg tcattagccc gctatttgcc agccaaccat
      481 ctttcactat ccatttttcg ttgtttagcc ataacggcaa tgcaccacta aatgccccgg
      541 acatggcgaa ttgtttcgga ttgacggcgc taaccagttc gctggatgag agattattca
      601 gccgcaacag cgccgggtca tgttgcggca tacgtaattg tttcatcagc acgttaccgc
      661 ctaacacatc gacgctaaca tcagtcaaca gcaagggttc ttcttcagtc cacggataac
      721 gcccttgcaa atcagccgta atatttttcg ctgtcaccag attaatcact tcggcaattc
      781 gcaacgtaac ggggccgcga gtccccagat gccaggctcc atcggcaaaa cggaaaggca
      841 ggacaaaatc gacaccgtta acctgattat ctggcatcca ggcactaccg cctttcaaca
      901 cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc agaaaatgca acctgagcat
      961 acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg caccagcggc tggaatacag
     1021 tcagtgattg ttttggccac caggcgttac cgcgcagacg aataccgtcc cagcgaccat
     1081 ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc tttaaagagg aaataggtcg
     1141 gattgcgccc atcgacgcta aattttaagg ttgatggcgg taacacactg ccgccagtga
     1201 acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa ggagggatgc tgtgcgtcac
     1261 gtacccagac gacgggcttg tcgagaatta atcgcggctt ttctaccgtc atcgtaccgt
     1321 attgtaactg gtcgaaaccg gtggagagat cggtcagcgt aatcgtgctg tcatgccact
     1381 caccttttcc tgcgacatcc caggtggcat tcatcggtgt aaaactccct tttccccagt
     1441 agcgccactg ccagcggcca gcgtcaggga gaaaatcatt cgccagccca tccatatgca
     1501 gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat ggcctgcaaa cgtccgtcaa
     1561 caccacgttg ggtgactttt acacccgcta aaggccagcg gatttcatcg atgtccagcg
     1621 aatcgatgac tcttcccttt gaacgaagta acgcgcctgg ctcaaaggtc agcgttgggt
     1681 cagacagact tccacttagc tgcgcaggta aacgggcata taaaatgagg tccgcctgtt
     1741 tcgcttcacc ggtcagctgc agaggcagtt gactgttatc catgcttaat tttcctgggc
     1801 caaaattaag caccgcgttg cccttacccg cttgcccctg ggtcagcaca ttcagtcgtc
     1861 cgctgaccag agcgttctca agccctgcct gccagttgtc gactttgaca cccagtcggc
     1921 cactcaaagg aaaaccagca tacagccagc tccagcgacc atcgcttacg gtcaattgtt
     1981 gacgagtaat ttgccacggc aaatcgagca acggatcgcc gttatcccgt gccagcacga
     2041 tcaattgccc gctattttcc tgccagtcca gctcggcatc caccagtgac ggttcctgcg
     2101 gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag tccatccggc acgagcggca
     2161 tagtaaattc ccccaccagt ttaaccggcg gctgattttc aaacgcgacg acatccagtt
     2221 cgctgactgt aagttgttgc cctttcagct ggccttgaaa tttaactttt tcgccctgat
     2281 aacgcagttg ctggatatca gaggttaatg cgagagagag ttttccctgc cattcctgcc
     2341 agggagaaaa aatcagttta tcgatattga tccaggtgtt aggcagcatg gactgccact
     2401 gcgcgagggt ttttggagca gctggcgatt gctccgtctg cggcaatttc gccagacaag
     2461 cagaatcaag ttctaccgtg ccgacgttca ataaccagcg gctgggatgt gaaaggctgg
     2521 cgttggtgat atgcgcaagc tgacaatctc ccaccagata acggagatcg ggaatgatta
     2581 aacctttacg cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg agcggtagcc
     2641 agatgtccgc cagcgtggga acccacagcc cgagcgtcat cagcagcgtc aacggcacaa
     2701 gaataatcag taataacagc gcgagaacgg ctttatattt acccagcatg ggtagttaat
     2761 atcctgattt agcgaaaaat taagcattca atacgggtat tgtggcatgt ttaaccgttc
     2821 agttgaaggt tgcgcctaca ctaagcatag ttgttgatga atttttcaat atcgccatgg
     2881 ctttcaatta tatttgaaat tttgtaaaat atttttagta gcttaaatgt gattcaacat
     2941 cactggagaa agtcttatga aactcgccgt ttatagcaca aaacagtacg acaagaagta
     3001 cctgcaacag gtgaacgagt cctttggctt tgagctggaa ttttttgact ttctgctgac
     3061 ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta tgtattttcg taaacgatga
     3121 cggcagccac ccggtgctgg aagagctgaa aaagcacggc gttaaatata tcgccctgcg
     3181 ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa gaactggggc tgaaagtagt
     3241 ccgtgttcca gcctatgatc cagaggccgt tgctgaacac gccatcggta tgatgatgac
     3301 gctgaaccgc cgtattcacc gcg
//
LOCUS       DAJTRH010000204         3258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTRH010000204 DAJTRH010000000
VERSION     DAJTRH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..442
                     /locus_tag="OO339_004015"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO339_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO339_004016"
     CDS             1026..1742
                     /locus_tag="OO339_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928425.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO339_004017"
     CDS             2294..2698
                     /locus_tag="OO339_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928426.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3258
                     /locus_tag="OO339_004018"
                     /pseudo
     CDS             2761..3258
                     /locus_tag="OO339_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          749 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaagg cctcccgg
//
LOCUS       DAJTRH010000205         3209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTRH010000205 DAJTRH010000000
VERSION     DAJTRH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..479
                     /locus_tag="OO339_004019"
     CDS             <1..479
                     /locus_tag="OO339_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5928427.1"
                     /translation="ENWPDMLENGTSLVMTGSCGTGKNHLAVAMAKHIIRNYLASVEI
                     TDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEVGVQFGSAAEMAILQEIIN
                     ARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWLSFNWPSYRSRIRGVAA"
     gene            476..1867
                     /locus_tag="OO339_004020"
     CDS             476..1867
                     /locus_tag="OO339_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5928428.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            1864..2121
                     /locus_tag="OO339_004021"
     CDS             1864..2121
                     /locus_tag="OO339_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928429.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2216..2416
                     /locus_tag="OO339_004022"
     CDS             2216..2416
                     /locus_tag="OO339_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5928430.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2409..2789
                     /locus_tag="OO339_004023"
     CDS             2409..2789
                     /locus_tag="OO339_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5928431.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            2989..3065
                     /locus_tag="OO339_004024"
     tRNA            2989..3065
                     /locus_tag="OO339_004024"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3023..3025,aa:Ile,seq:cat)
     gene            3067..3143
                     /locus_tag="OO339_004025"
     tRNA            3067..3143
                     /locus_tag="OO339_004025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3101..3103,aa:Arg,seq:tct)
BASE COUNT          837 a          708 c          926 g          738 t
ORIGIN      
        1 cggagaactg gccagatatg ctggagaacg gtaccagtct tgttatgacc ggcagttgtg
       61 gtaccggaaa aaatcatctg gctgtggcta tggcaaaaca catcatccgt aactatctgg
      121 ccagtgtgga gatcaccgac gtgatgcgcc ttacccgtgc tgtgaaaaat tgctggcgga
      181 atgacagcga aaaaacagcg gatgaagtta ttgagcgtta tgcgtcaatg gatttgctga
      241 tcatcgacga agttggcgtt cagtttggca gcgcggctga aatggctatt ttgcaggaaa
      301 ttatcaacgc caggtacgaa agtatcttgc ccaccattct gatcagtaac ctctcaccgg
      361 aagagttgtg ggcgttcatc agtccccgaa ttgccgacag gatcacagac gggggacgca
      421 actggttgtc gtttaactgg cccagttacc gttctcgtat cagaggtgtg gctgcatgac
      481 aactccagta tggcgtaacg atgaccttga aggcgctgtc atcggcgcgt tctttctgcg
      541 cggagcagat cctgaagtga tggatattct ggccacactg ccagcggacg ttttttctgt
      601 acgagcgtat caggatatct atacaggcat ctgcagacag gcccgtgttt caggagtgat
      661 tgaccccgtg ctgttgtgta atgagatgcc ggaacttgcc ccggtgatta ctgataccgg
      721 gcgtaaaacc tgggtgaagt cttcactgga gcactatgtt gcagcgttgc ggcgcaatgc
      781 cgcactgcgc gatgcagaaa aaacactgaa tgaggcgctg cagaaattac gtgatgcgca
      841 tacctgtgaa gcagctgaag atgccctgaa ggatgcgcag aacatgatgg tcacactgtc
      901 gacaggaaag ggcgtcattc agccggtaca tattgatgat gtgcttccgg aagtggttga
      961 gcgtgttgaa tgccggaatc aggggctgga gaaatccagg acgttgatga ccggtattga
     1021 tgaactggac gcaaaaacag gcggtatgga gcccggagac ctggtattca ttgccgcccg
     1081 tccttcgatg gggaaaaccg aacttgcgct ggacatcatc gacaaggtga ctgagcaggg
     1141 gcatggtgtg cttctgttca ccatggagat ggcgaacatc cagattggtg aacgtatggt
     1201 gtctgcggct ggagggatgc cagtatcacg cctgaaatct gtggctcact ttgaagatga
     1261 agactggacg cgtttctcac aaggggtggg gcggatgacc gggcgcaata tctggatggt
     1321 ggaccaggcg aacctggcca ttgatgagat atgcgcaaca acgaaacacc acctgattaa
     1381 atatccggaa acggcgctgg tggtggttga ttatctcggg ctgataaaaa cccgaaccac
     1441 ggggcgtcat gaccttgccg tgggtgaaat ctcaaagggg cttaaaggcc tggcaaaatc
     1501 cggtggtttt ccgttgattg cgctgagcca gctctcccgc ggtgtggagt ccagacccaa
     1561 taaacgtccc atgaactcag acctgaaaaa ttccggagaa atagaggcgg atgcagacat
     1621 cattctgatg ctttacaggg atgaagtgta caacccggat acgcaggcca ggggcatcgc
     1681 agaaatcaat atcacgaaac aacgtaacgg ttctctggga acgatttacc ggcgttttta
     1741 taacggacat tttctgcctg tggaccagga aagtgcacgg gtgctttcca ctcccatgaa
     1801 gcccggcaat ccgcgcagat acagtaataa acgaactgac agcagtaaga tggagcgttt
     1861 cttttgaaca accagacaat gacttttacc cctgaacaat tgcgtaaaca cgcgcaggaa
     1921 atgctgcgtc atgctgaaca actcgaaaaa acgggcataa caaaagatgc tatccgtaaa
     1981 gatatggtac ccgcgcttcg ggaactgatg caggcgaagc atcgcgcaca gaaagcagta
     2041 gatgagctgg tggactgtgt ggcagagctg gaaaccagag tcggaaagtt tgaaaaactg
     2101 gtgcaggagg cgctgcgctg atgcgccatg agtttgtttt gccctggctt gtgcgcgatc
     2161 attatgtccc tggtggaaag cccgggataa aaatcaccga accggagagt atttgatgag
     2221 cgtcaaaatt caaacgatac cggaactgct tatccagaca cgaggtaata tgaccgaagt
     2281 ttcacgaatg ctgaactgta atcgcgccac ggtaagaaaa tatgctgaag ataaagaggg
     2341 caaagggcac gccattgttg atggtgttct gattgttcat cgtggatggg acaggggtaa
     2401 agacagtgat gcgtgatatt cagcaggttc ttgagcgctg gggcgcgtgg gtggctaata
     2461 atcatgagga tgtgacctgg tcgtccattg ctgccggttt taagggatta atcccttcaa
     2521 aagtaaaatc ccgcccgcaa tgctgtgacg atgacgcgat ggttatatgt gggtgtatgg
     2581 cccgccttaa ccggaacaac aacgatctac atgacttgct ggttgactac tacgtgtttg
     2641 ggatgacatt catgacgctg gcgcggaagc atggatgctc agatggttat ataggaaaga
     2701 agctacaaaa agccgagggg gtggtggaag gaatgttgat gatgttgggt gtccggttag
     2761 aaatggacaa atatattgaa catccctgat ggtaaggggc gactttctag aaaatatttt
     2821 acgtacgtaa aaaacgggat attctgttaa gagtggttgc ttcgccacac agcttaaacc
     2881 cgcctccggg cgggtttttt atgcctgaaa aacggtacag gacgttaaac acgctggtgg
     2941 tcagatgagt ttgcagatgt gatgacatat ggttattatt ctgcctccgg ccctttagct
     3001 cagttggtca gagcgagcga ctcataatcg ccaggtcgct ggttcaagtc cagcaagggc
     3061 caccaaccac cactagctca tccggataga gcatcaacct tctaagttga cggtgcgagg
     3121 ttcgagtcct cggtggtggg ccagcgccga cttagctcag caggcagagc aactgacttg
     3181 taatcagtag gtcaccagtt cgattccgg
//
LOCUS       DAJTRH010000206         3181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTRH010000206 DAJTRH010000000
VERSION     DAJTRH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            88..2991
                     /locus_tag="OO339_004026"
     rRNA            88..2991
                     /locus_tag="OO339_004026"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3087..>3181
                     /gene="rrf"
                     /locus_tag="OO339_004027"
     rRNA            3087..>3181
                     /gene="rrf"
                     /locus_tag="OO339_004027"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          843 a          700 c          987 g          651 t
ORIGIN      
        1 gaaacactga acaacgaaag ttgttcgtga gtctctcaaa ttttcgcaac acgatgatga
       61 gtcgaaagaa acatcttcgg gttgtgaggt taagcgacta agcgtacacg gtggatgccc
      121 tggcagtcag aggcgatgaa ggacgtgcta atctgcgata agcgtcggta aggtgatatg
      181 aaccgttata accggcgatt tccgaatggg gaaacccagt gtgattcgtc acactatcat
      241 taactgaatc cataggttaa tgaggcgaac cgggggaact gaaacatcta agtaccccga
      301 ggaaaagaaa tcaaccgaga ttcccccagt agcggcgagc gaacggggag cagcccagag
      361 cctgaatcag tgtgtgtgtt agtggaagcg tctggaaagg cgtgcgatac agggtgacag
      421 ccccgtacac aaaaatgcac atactgtgag ctcgatgagt agggcgggac acgtggtatc
      481 ctgtctgaat atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga
      541 accagtaccg tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga
      601 aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg tgactgcgta ccttttgtat
      661 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
      721 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      781 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      841 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      901 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      961 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
     1021 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
     1081 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
     1141 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
     1201 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacgct
     1261 tatgcgttgt tgggtagggg agcgttctgt aagcctgtga aggtgtgctg tgaggtatgc
     1321 tggaggtatc agaagtgcga atgctgacat aagtaacgat aaagcgggtg aaaagcccgc
     1381 tcgccggaag accaagggtt cctgtccaac gttaatcggg gcagggtgag tcgaccccta
     1441 aggcgaggcc gaaaggcgta gtcgatggga aacaggttaa tattcctgta cttggtgtta
     1501 ctgcgaaggg gggacggaga aggctatgtt ggccgggcga cggttgtccc ggtttaagcg
     1561 tgtaggctgg ttttccaggc aaatccggaa aatcaaggcc gaggcgtgat gacgaggcac
     1621 tacggtgctg aagcaacaaa tgccctgctt ccaggaaaag cctctaagca tcaggtaaca
     1681 tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag aataccaagg cgcttgagag
     1741 aactcgggtg aaggaactag gcaaaatggt gccgtaactt cgggagaagg cacgctgata
     1801 cgtaggtgaa gcgacttgct cgtggagctg aaatcagtcg aagataccag ctggctgcaa
     1861 ctgtttatta aaaacacagc actgtgcaaa cacgaaagtg gacgtatacg gtgtgacgcc
     1921 tgcccggtgc cggaaggtta attgatgggg ttagcgcaag cgaagctctt gatcgaagcc
     1981 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
     2041 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga
     2101 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc
     2161 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggctttg
     2221 aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta atgtttgatg
     2281 ttctaacgtg gacccgtgat ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg
     2341 cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg gtcggacatc
     2401 aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg cgagcaggtg
     2461 cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct caacggataa
     2521 aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac ggcggtgttt
     2581 ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag ggtatggctg
     2641 ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag ttcggtccct
     2701 atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag gaccggagtg
     2761 gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc taaatgcgga
     2821 agagataagt gctgaaagca tctaagcacg aaacttgccc cgagatgagt tctccctgac
     2881 cctttaaggg tcctgaagga acgttgaaga cgacgacgtt gataggccgg gtgtgtaagc
     2941 gcagcgatgc gttgagctaa ccggtactaa tgaaccgtga ggcttaacct tacaacgccg
     3001 aagatgtttt ggcggattga gagaagattt tcagcctgat acagattaaa tcagaacgca
     3061 gaagcggtct gataaaacag aattagcctg gcggccgtag cgcggtggtc ccacctgacc
     3121 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
     3181 c
//
LOCUS       DAJTRH010000207         3180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTRH010000207 DAJTRH010000000
VERSION     DAJTRH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO339_004028"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO339_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5928432.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO339_004029"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO339_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          800 a          659 c          776 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtgctga ctattttcat
//
LOCUS       DAJTRH010000208         3170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTRH010000208 DAJTRH010000000
VERSION     DAJTRH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..73
                     /locus_tag="OO339_004030"
     CDS             <1..73
                     /locus_tag="OO339_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5928433.1"
                     /translation="HLGYREVTITEKGYAAFKTHFHI"
     gene            complement(51..674)
                     /gene="tetR(B)"
                     /locus_tag="OO339_004031"
     CDS             complement(51..674)
                     /gene="tetR(B)"
                     /locus_tag="OO339_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5928434.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            756..1961
                     /gene="tet(B)"
                     /locus_tag="OO339_004032"
     CDS             756..1961
                     /gene="tet(B)"
                     /locus_tag="OO339_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5928435.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2074..2667)
                     /gene="tetC"
                     /locus_tag="OO339_004033"
     CDS             complement(2074..2667)
                     /gene="tetC"
                     /locus_tag="OO339_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5928436.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2800..>3170
                     /locus_tag="OO339_004034"
     CDS             2800..>3170
                     /locus_tag="OO339_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5928437.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          862 a          581 c          645 g         1082 t
ORIGIN      
        1 acatcttggt taccgtgaag ttaccatcac ggaaaaaggt tatgctgctt ttaagaccca
       61 ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc gaataagaag
      121 gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata
      181 ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat
      241 acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa
      301 aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta
      361 ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa
      421 cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga
      481 gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg cttatttttt
      541 acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt acgggttgtt
      601 aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta
      661 tctaatctag acatcattaa ttcctaattt ttgttgacac tctatcattg atagagttat
      721 tttaccactc cctatcagtg atagagaaaa gtgaaatgaa tagttcgaca aagatcgcat
      781 tggtaattac gttactcgat gccatgggga ttggccttat catgccagtc ttgccaacgt
      841 tattacgtga atttattgct tcggaagata tcgctaacca ctttggcgta ttgcttgcac
      901 tttatgcgtt aatgcaggtt atctttgctc cttggcttgg aaaaatgtct gaccgatttg
      961 gtcggcgccc agtgctgttg ttgtcattaa taggcgcatc gctggattac ttattgctgg
     1021 ctttttcaag tgcgctttgg atgctgtatt taggccgttt gctttcaggg atcacaggag
     1081 ctactggggc tgtcgcggca tcggtcattg ccgataccac ctcagcttct caacgcgtga
     1141 agtggttcgg ttggttaggg gcaagttttg ggcttggttt aatagcgggg cctattattg
     1201 gtggttttgc aggagagatt tcaccgcata gtcccttttt tatcgctgcg ttgctaaata
     1261 ttgtcgcttt ccttgtggtt atgttttggt tccgtgaaac caaaaataca cgtgataata
     1321 cagataccga agtaggggtt gagacgcaat cgaattcggt atacatcact ttatttaaaa
     1381 cgatgcccat tttgttgatt atttattttt cagcgcaatt gataggccaa attcccgcaa
     1441 cggtgtgggt gctatttacc gaaaatcgtt ttggatggaa tagcatgatg gttggctttt
     1501 cattagcggg tcttggtctt ttacactcag tattccaagc ctttgtggca ggaagaatag
     1561 ccactaaatg gggcgaaaaa acggcagtac tgctcggatt tattgcagat agtagtgcat
     1621 ttgccttttt agcgtttata tctgaaggtt ggttagtttt ccctgtttta attttattgg
     1681 ctggtggtgg gatcgcttta cctgcattac agggagtgat gtctatccaa acaaagagtc
     1741 atcagcaagg tgctttacag ggattattgg tgagccttac caatgcaacc ggtgttattg
     1801 gcccattact gtttgctgtt atttataatc attcactacc aatttgggat ggctggattt
     1861 ggattattgg tttagcgttt tactgtatta ttatcctgct atcgatgacc ttcatgttaa
     1921 cccctcaagc tcaggggagt aaacaggaga caagtgctta gttatttcgt caccaaatga
     1981 tgttattccg cgaaatataa tgaccctctt gataacccaa gagggcattt tttacgataa
     2041 agaagattta gcttcaaata aaacctatct attttattta tctttcaagc tcaataaaaa
     2101 gccgcggtaa atagcaataa attggccttt tttatcggca agctctttta ggtttttcgc
     2161 atgtattgcg atatgcataa accagccatt gagtaagttt ttaagcacat catcatcata
     2221 agctttaagt tggttctctt ggatcaattt gctgacaatg gcgtttacct taccagtaat
     2281 gtattcaagg ctaatttttt caagttcatt ccaaccaatg ataggcatca cttcttggat
     2341 agggataagg tttttattat tatcaataat ataatcaaga taatgttcaa atatactttc
     2401 taaggcagac caaccatttg ttaaatcagt ttttgttgtg atgtaggcat caatcataat
     2461 taattgctgc ttataacagg cactgagtaa ttgtttttta tttttaaagt gatgataaaa
     2521 ggcacctttg gtcaccaacg cttttcccga gatctcatct attgaaacag cttgatagcc
     2581 tttttcaaca aacaatattc gtgctgagtt aaccagtgat tgataggtac tcttaaaatt
     2641 ttcttgttga tgatttttat tttccatgat agatttaaaa taacataccg tcagtatgtt
     2701 tatggtatca tgatgatgtg gtcgtgacaa tcttaagaac atttaggtta ttttatgtat
     2761 attgaacagc attctcgcta tcaaaataaa gctaataaca tccaattaag atatgatgat
     2821 aagcagtttc atacaacggt tatcaaagat gttctattat ggattgaaca taatttagat
     2881 cagtctttac tgcttgatga tgtggcgaat aaagcgggtt ataccaagtg gtattttcag
     2941 cggctgttca aaaaagtaac aggggtcaca ctggctagct atattcgtgc tcgtcgtttg
     3001 acgaaagcgg ctgttgagtt gaggttgacg aaaaaaacta tccttgagat cgcattaaaa
     3061 tatcaatttg attcccaaca atcttttaca cgtcgattta agtacatttt taaggttaca
     3121 ccaagttatt atcggcgtaa taaattatgg gaattggagg caatgcactg
//
LOCUS       DAJTRH010000209         3139 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTRH010000209 DAJTRH010000000
VERSION     DAJTRH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(146..1855)
                     /locus_tag="OO339_004035"
     CDS             complement(146..1855)
                     /locus_tag="OO339_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5928438.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2287..3006)
                     /locus_tag="OO339_004036"
     CDS             complement(2287..3006)
                     /locus_tag="OO339_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5928439.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          963 a          597 c          530 g         1049 t
ORIGIN      
        1 tttcaggaag gagtgtccgt taaactcagg ctacctcagg ttgccaattg aggtggttgc
       61 ctgaaagttg gcagttaccc ggtcactaca ccttgttgga gtgaacatat caaaaacagg
      121 taattacaca atttaaatta cagtctcata aaaaatgatt aaatctattt attatttcaa
      181 taaacgtttt tttcatcttc tttgaaaatt gttctgctat agtatccggt tccagattgt
      241 tctcttttat tattctacaa tactctaata ttgtttcttt gtctctattt actacagata
      301 caaatacacc aggaatacca tctttattag ttgcggacag catttcgacc aatcgtcttg
      361 tggtaaaact atagtttttt agaatattac aaaaggcacg tattgtttcg gtatggccat
      421 tataaaatgc cataaaaagt cctgatgtac cgttgggatt ttttatccat aataattcat
      481 ctatatattc ctggtgaggg gggagtattt ttagtatatc tccatacgcc ataatggcct
      541 ttgtgtgccc attttgaagt gccataaaca acccatgctt tactttcaat aattctttaa
      601 tttgttcact actaagcatt ttcttattaa gcagacttcc gaacatacga atactatctg
      661 catgtccatt ctgcaatgca aaaaataacc caggtaaatc atggctgtct ttcgcagcta
      721 atagatataa tctcttatcg gaagttaacg gggttttaaa gactatttcc ccataggctt
      781 ttattgtgtc tacatgtccg ttcataaaag acatgcataa acccggcaca ttgtttgccc
      841 ctaccgcagc aagcaatgta agtattgttt cttgactaag attgcacata tttaatatct
      901 taccatatac ttgaataatg tctgaatatc cgttttgaag tgctatataa aatgcaggag
      961 taccatcaga gcttctcgtg gccagaaggt tcaccagttt agattgagaa atttcaccat
     1021 ttttgtattt ttccatcagt ataaataata cttctgtcag attctttgtt atcgcatgat
     1081 atataactct ggaattgatc aattcatctg gtaaaagttt cactgtgttt gttagagggg
     1141 aattatgcct atcttcaaat attacctgcc cgggttgctc cagaccataa attttccaat
     1201 aaaaatcatc aaaaaaatcc ttagcggata attgactcag ttcgaaatgg ctatcacaag
     1261 taaatacagt acgtttatga gtaacagttc gattaggatc gaaaaactga acaacccatt
     1321 tttcactttc ttgtacgcgc tttattttca accctaacgc catttggtgg tttaatgtgc
     1381 atgctgataa tgttctgaca aagtctccat tttttttcat gttataaaaa aaattagtaa
     1441 taactgttcc ccacttttta ttttccacaa aataaatata agctggtgat tttgaaaaat
     1501 cttccgtttt ttccaaacat acattttttg caatagcatc aggggtgcta agaagctgat
     1561 agtttacctt cccatttgtt tccataaaat tcatttccca ataggaagct aggtgacggc
     1621 aaactatttg tcttccatat atatcttttg ccactccatt tatatttacc tccacatttc
     1681 tcttttctga aaaataaggt gctgttttat tcttaaactg aattagttca ctggagtttg
     1741 agaccctcat aatagaactt gttattgttc tattatctct gttaacagac atatctgttg
     1801 ataacgaatt ttttgttgaa aatatactag atgaaaatgt tttatttaac ggcatagaag
     1861 acaaccatag agatgttata atactattag attgcagtta ctaatagtaa aagcctcctt
     1921 acaaggaggt agcttattca gaatgctcct ataagatagc gagctcgtca acgatacaga
     1981 tgatctgatt atattgtgca aaaggagaac aggaaaatag gcactggaaa agatgaaggc
     2041 ctgatgggag cattaactaa tgcaaaatcc gacaggttaa tatcccgaaa agcaatcaaa
     2101 cgactcaaac aggagatgca cgctgaaatg gtggacaact gttcggattt cagatgagta
     2161 gctcagcagt ttatgagccg aatgctctgt cacgggcagg aatcaggctg catacgcagt
     2221 gccaaatcta atcgtagtta ttctaaccag ttttcaagct acaaggtggt gtagctggca
     2281 tcttctctac tctatcatca aacgataaaa tggttcctct cgtaacattt gctcttgcct
     2341 ttcacttcca tctcgatcgc ttcgtagttc attacgatag ctgacatatt tccagtcttc
     2401 aggtctaaca tctgacgccg gatactcccc gggagcgatt ttgcttctta atagattaga
     2461 ctcgagacac attataaatt gtcgtatttt atgaagtaat aaagcactat attgcgagca
     2521 ttcctgatct gattttacat atagcgtaaa ctgagctcct atccccacac gagattgctg
     2581 agaaactcga ttcatatcag ttatcttcca tttatctata ggactgtcct ctgaaaataa
     2641 taaaccagac aaaatttgga atgccagagg aacttgctcc ctagctatac taatatgaaa
     2701 cttgtcccca acaaaatccc ccttactctg aggattttca cgacatgcat gaataaacac
     2761 atctccacta tggttcacaa tctggaatcc ttggtacatg acagaaaaag attgacatat
     2821 atcagagtaa gttggcacgt tcgatgctac tttgaaatag tacgctgggt attgccgtat
     2881 ttggttgtac aatatattga agttattttt caatctttca ttcgcagaaa gcatctgcgg
     2941 tatttcagac ctcacaacat tcaaagaatc taaatttagt tttagacagg gcttttttat
     3001 gggcatagaa aacgtcctct ataaaataga tatattatct tatattgatt gtttatcatc
     3061 ttttataaaa cgatacatta tatgtaatgt atgtaagcgt aagggtcctc tccgaacatg
     3121 gtgggaaggc ttagaacgc
//
LOCUS       DAJTRH010000210         3132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTRH010000210 DAJTRH010000000
VERSION     DAJTRH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(107..262)
                     /locus_tag="OO339_004037"
     CDS             complement(107..262)
                     /locus_tag="OO339_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5928440.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(465..872)
                     /locus_tag="OO339_004038"
     CDS             complement(465..872)
                     /locus_tag="OO339_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5928441.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            949..1176
                     /locus_tag="OO339_004039"
     CDS             949..1176
                     /locus_tag="OO339_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5928442.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1160..1582
                     /locus_tag="OO339_004040"
     CDS             1160..1582
                     /locus_tag="OO339_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928443.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1660..2437
                     /locus_tag="OO339_004041"
                     /pseudo
     CDS             1660..2437
                     /locus_tag="OO339_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2444..3132
                     /locus_tag="OO339_004042"
                     /pseudo
     CDS             2444..3132
                     /locus_tag="OO339_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          805 a          684 c          866 g          777 t
ORIGIN      
        1 cagccagatg cgtgccccag tgcctgcatt tggctccagt tgctggaggc gttttgcatc
       61 ttctaggagt agagcgataa cgtgttttaa ttctgtctcg ttcattttac tcacctgaat
      121 gtcttcccaa ccaacgacgt gcgccagctt cggttttaaa cgttttgctt ttggtatacg
      181 ttatggcggt gaatgtgccg tcctggttgg ggaacacgcc gtacaccgga gattcgttgt
      241 tgccaagatc gatagtattc atgttgaccc catttcccct taacgccggg gtagcggaac
      301 tgtttgctga gaacaccgtg cggtgtcttg atgaataaaa tttagaataa cctaatgagg
      361 gtgatcaaga tttttgtgta gaaaaaccta agttttttga tgtaaaaaac acaagtgttt
      421 gaaagtttgt gctttttatt acagggtgtg gagaaaaaag gggattattt gtttgcgctt
      481 cttttgcgag ctttgagtag ttcttcaaaa agtttgttga aattttcaac tcgagcacgc
      541 atctctgaca acagggcctt ttgctctgac tcaggcagtg cgtcgaacag ttgaagtaac
      601 tctttttgat cttctgtcag aatggctggc tgattatccg ggatcggttc gcctggttgt
      661 ttatcttcat ccccaaaaag aagccaagtc ggtgagcact gaagcgcttg gctcagtgcg
      721 aataatcttt tccccgccgg ctgtgtttca tctctttccc attgagaaat tgttacgtga
      781 gccactttga ccagcttacc taatgcggcc tgagacagtt ttaatttttt acgcctatgt
      841 aagaggcgag caccgaaggt ttcgtttttc atattaggga attctaattt ttcttgactt
      901 aggtttctct acggcctggt ttccttagga aaatctaagg agctcgatat gttgaaaatt
      961 gatgctatag cgttttttgg cagcaaaaca aagcttgcca atgccgcagg agttaggctg
     1021 gcaagcattg ctgcatgggg ggaactggtt cctgaaggtc gcgcgatgcg cctgcaagag
     1081 gcatctggcg gggaacttca gtacgacccc aaagtttatg acgaatatcg taaggcaaaa
     1141 cgacctggga aggtgattca tgaaaatcag gcatgagcac atcgaatcag tgctgttagc
     1201 cctggcagcc gaaaaagggc aggcgtgggt cgctaacgca attactgaag aatatctgcg
     1261 gcaggggggc ggcgaattgt ctctggtacc aggcaaggac tggaacaatc agcagaatat
     1321 ctatcaccgt tggttgaaag gtgaaacgaa agcgcaaagg gaaaaaattc agaaactgat
     1381 ccctgcggtt ctggcaattc ttccgcgtga gctgcgtcac cgactctgta tcttcgatac
     1441 cctggaacgc cgtgcattac tggcggcgca ggaagcgttg agtacggcaa ttgatgcgca
     1501 tgatgatgct gtccaggccg tttaccggaa agcacatttc agcggtggtg ggtcgcccgg
     1561 cgattctgtc gtagtgcatt gattgaaatt aatcgtgccg gattgttttg ttcggtatca
     1621 gttaaatgta acgctgcgag cgttacaagg tgaaaacaaa tggcttcaaa ctggataaag
     1681 ctcgaggtta ttacgccgga taagccggaa atattcaggc ttgctgagat tctgaatatt
     1741 gatccagatg ccgcattagg gaaggttatt cgcttctggg catgggcgga tcaacaaatg
     1801 atagacggta atgcagattg taacgctcgc ggcgttacaa aaagtgcaat agatcgcatc
     1861 acttttatgt ctggttttgc tgatgcgtta attcaggttg gatggctggt cgaaaatgac
     1921 ggtgggcttt ctctacctaa ctttgaacgt cataacggaa aaagctctaa aaaacgggcg
     1981 gttacaaacg agcgagtaac aaaaatacgc gaactgaaac gaaaaggtaa cgctgccagc
     2041 gttacacaaa cggatcaaaa agcgttacca gtggaagagg aagaggaaga tctaaatact
     2101 gatctccccc taaatccccc tcgccaaaaa cgagcgtcta aaaaattcga gccggaggct
     2161 attgagctgc ccgattggtt gccggaaaca ctctggcatg agtgggtccg gttcagacag
     2221 gcattgcgaa aaccgattcg aacggagcag ggcgatacgg gaactggaaa aattccgtca
     2281 gcagggtttt acacctgagc aggtgattcg acacagcatc gccaatgaat accagggcct
     2341 gttcgcgccg aaaggtgttc ggcctgagac gttgctccga caggttaaca ccgtctcgtt
     2401 gccggacagt gcgatcccgc caggcttcag ggggtaacgg accatgaaaa atattgcgac
     2461 aggcggcgtt ctggaacgca tccgccgact gaccccgcaa catgtaaccg ccccattcag
     2521 aacggtagcg gagtggcgcg agtggcaact tgctgaaggc cagaaacgta gcgaggagat
     2581 caaccgcctg aatcgccagt tgcgggtgga aaaaattctg aatcgctcag gcatccagcc
     2641 gttgcaccgt aaatgctcgt ttgcgaatta ccaggtgcag aacgacggcc agcgatacgc
     2701 gttaagccag gcgaaatcca tcgccgatga actgatgacc gggtgtacaa attttgcgtt
     2761 cagcggaaaa cctgataccg ggtagaatca cttagcggca gctatcggga atcgcctgct
     2821 gaaagacggt cagacagtga ttgtggttac cgtggctgat gttatgagcg ccctgcacgc
     2881 cagctatgac gacgggcagt caggcgaaaa atttttgcgg gagctgtgcg aagtggatct
     2941 gctggttctt gatgaaattg gcattcagcg cgagacgaaa aacgagcagg tggtgctgca
     3001 ccagattgtt gatcgccgga cagcgtcgat gcgcagcgtg gggatgctga caaacctgaa
     3061 ctatgaggtg atgaaaacat tgctcggcga gcgggtgatg gatcgcatgg tcatgaacgg
     3121 cgggcgctgg gt
//
LOCUS       DAJTRH010000211         3113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTRH010000211 DAJTRH010000000
VERSION     DAJTRH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO339_004043"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO339_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5928444.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO339_004044"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO339_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO339_004045"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO339_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5928445.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO339_004046"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO339_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5928446.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3113)
                     /locus_tag="OO339_004047"
                     /pseudo
     CDS             complement(2163..3113)
                     /locus_tag="OO339_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          758 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag ggg
//
LOCUS       DAJTRH010000212         2790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTRH010000212 DAJTRH010000000
VERSION     DAJTRH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            437..2089
                     /locus_tag="OO339_004048"
     CDS             437..2089
                     /locus_tag="OO339_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5928447.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO339_004049"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO339_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO339_004050"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO339_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          833 a          570 c          644 g          743 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc
//
LOCUS       DAJTRH010000213         2663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTRH010000213 DAJTRH010000000
VERSION     DAJTRH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..632
                     /gene="lsrF"
                     /locus_tag="OO339_004051"
     CDS             <1..632
                     /gene="lsrF"
                     /locus_tag="OO339_004051"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5928448.1"
                     /translation="DVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALS
                     MDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQ
                     RYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQA
                     IDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            656..946
                     /gene="lsrG"
                     /locus_tag="OO339_004052"
     CDS             656..946
                     /gene="lsrG"
                     /locus_tag="OO339_004052"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5928449.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            986..1744
                     /gene="tam"
                     /locus_tag="OO339_004053"
     CDS             986..1744
                     /gene="tam"
                     /locus_tag="OO339_004053"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5928450.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1748..2662)
                     /locus_tag="OO339_004054"
     CDS             complement(1748..2662)
                     /locus_tag="OO339_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5928451.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          698 a          619 c          721 g          625 t
ORIGIN      
        1 ccgatgtatt aatgtgtacg cgcggcattt tgcgcagcgt agttcccccg gcgaccaata
       61 agccggtggt actgcgggcg tcaggtgcga actctattct ggcggaatta agtaatgaag
      121 ccgtggcgtt atcgatggat gatgccgtgc gcctgaacag ttgcgcggtg gcggcgcagg
      181 tttatatcgg cagcgaatat gaacatcagt cgatcaaaaa tattattcag ctggtcgatg
      241 ccggaatgaa agtgggaatg ccgaccatgg ccgtgactgg cgtgggcaaa gatatggtgc
      301 gcgatcagcg ttatttctcg ctcgcgactc gaatcgccgc tgaaatgggg gcgcaaatta
      361 tcaaaaccta ttatgtcgaa aaaggttttg aacggattgt tgccggatgt ccggtaccca
      421 ttgttattgc tggcggtaaa aaattaccgg agcgcgagac gctggaaatg tgctggcagg
      481 ctatcgatca gggcgcttct ggtgtggata tggggcgtaa tattttccag tctgaccatc
      541 cggtggcgat gatgaaagcc gtacaggcgg tggttcacca taacgaaacg gctgatcggg
      601 catatgaact ctatctgagt gaaaaacagt aactgcggat ttaaggagaa gaattatgca
      661 cgtcacactg gttgaaatta acgttcatga agacaaggtt gacgagttta tcgaagtttt
      721 tcgccagaac cacctgggct ctgtacagga agaaggtaat ttgcgcttcg atgtcttaca
      781 ggacccggaa gtgaattcgc gcttttatat ttacgaagcc tataaagatg aagacacagt
      841 ggcattccat aaaaccacgc ctcactacaa aacctgtgtc gcgaaactgg aatctttaat
      901 gaccgggccg cgtaaaaaac gtctgttcaa tgggttgatg ccgtgaggct acaattgggg
      961 taacgcgctg acgggagtaa aaaaaatgtc tgactggaac ccctctttat atctgcactt
     1021 ttccgctgaa cgatcgcgtc cggcggtgga gctgcttgcc agagtgccgc tggagaatgt
     1081 cgaatatgtt gccgatcttg gctgtggccc aggtaacagc accgccctgc tgcatcaacg
     1141 ttggcctgcg gccaggataa caggcatcga ctcgtctccg gcgatgattg ctgaagcgcg
     1201 cagtgctttg ccagactgcc agttcgtgga agcagacatc cgcaactggc aaccggaaca
     1261 ggcactcgat ctgatttttg ctaatgcctc actgcaatgg ctgcccgacc actacgaatt
     1321 gtttcctcac ttggtttctt tacttaatcc gcagggcgtg ctggcagtac agatgccaga
     1381 taactggctg gagccgaccc atgtgctcat gcgcgaagtt gcttgggaac aaaactaccc
     1441 ggatcgcggg cgtgagccgt tggctggcgt tcatgcttac tacgatattt tgagcgaagc
     1501 cggatgtgag gtcgatatct ggcgaaccac ttactatcac cagatgccgt cgcaccaggc
     1561 gattatcgat tgggtgaccg ccactggatt acgtccgtgg ttacaggatc tgaccgagag
     1621 cgaacagcag ctttttctta cgcgctacca tcagatgctg gaagagcagt atccactgca
     1681 agagaacgga cagatactgc tggcatttcc gcgtctgttt attgttgccc gccgtacgga
     1741 gtaattatca cgtcagctgg taatgacgat cgggaagaat ttttgctgga atttctgctt
     1801 catcgttcat ctgtaacagg tcaatttcaa tagcgttgca gatggcatcc agcggtaaat
     1861 cattgttttc agtaccgaac ggatcttcca gttcttccgc cagacaatcc agcgaaataa
     1921 aagtgtagga aatcagcaca gagataaaag gcgtcatgta atgcaggtcc acgaccagcg
     1981 cgaacggcag catgatacaa aacagataaa cggtacgatg caaaatcagg gtgtaggcaa
     2041 agggaattgg cgtgtaggca atgcgctcac agcctgccag aaccgctgaa atatcattaa
     2101 gccgatcgtt gaggctaata aacaggatat ctgaaagctg tccattgcgg cgctgaaccg
     2161 ccaaccattc tcccattatt aacaagatac ggttagctgg agagttcgaa gccagtacac
     2221 gctgaagatc tccagtcttg agataatgag ccagcacttc cgcctgtggc tgtttgcgta
     2281 atgtcatgcg taaacagtgg gcgaaggcga tttgcagcct ggcaaactcc cttacacttg
     2341 ccgaatccgg caatgtcgtt tttacctctc gcagtaacga ccgtgaggca atcatcaact
     2401 gcccccaaag ttttcgcgct tcaacgtaac gggcgtaccc ggcattatta cgaaaaccaa
     2461 gaaaaatggc gatggcgaca ccgagaatgc tgaacggtgc gagggtgaat ttgatgccca
     2521 gatgcgtgta ccagggcagc atgaatataa cagcaataga aaagagaaaa ttgagtagta
     2581 agcgcgagga tatcttggat aatactgagc cgtgccagac aaaaatacgg cgcagccagt
     2641 gttgttgtgg acgaacaatc atg
//
LOCUS       DAJTRH010000214         2628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTRH010000214 DAJTRH010000000
VERSION     DAJTRH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            137..832
                     /locus_tag="OO339_004055"
     CDS             137..832
                     /locus_tag="OO339_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5928452.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            861..>2628
                     /locus_tag="OO339_004056"
     CDS             861..>2628
                     /locus_tag="OO339_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5928453.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          770 a          620 c          579 g          659 t
ORIGIN      
        1 gaaatcaccc caggggaagc agacgctgtc gttaacgtaa cgctggatta ccgttaatac
       61 gttacggcgt tatctgacct ttcagataac gcccttttcc ttcctctttc tcgttgtatc
      121 aggttgaaaa ataactatgt caaaacgaac attcgcggtg atattaacct tgttgtgtag
      181 cttctgtatc ggccaggcgc ttgcaggggg tatcgtttta cagcgaacgc gagtgatcta
      241 tgatgccagc cgcaaagagg ctgcgttacc tgtcgcaaac aaaggcgcag aaacgcctta
      301 tttactgcaa tcatgggtag ataatataga tggtaaaagc cgtgccccat ttattataac
      361 cccaccgcta tttcgtcttg aggctagcga tgactcatca ctgcgaatta ttaaaacagc
      421 tgataacctg cctgaaaata aagagtcgct gttctacatt aatgttcgtg ccattccagc
      481 aaagaaaaaa tcagatgatg ttaatgctaa cgagttgacg ctggtattta aaacgcgggt
      541 caaaatgttt tatcgccccg cacacctgaa gggacgggta aacgatgcgt ggaaatcact
      601 ggaatttaaa cgtagtgacc attcactcaa tatatataac ccaactgaat attacgtcgt
      661 atttgccgga ctggcagtcg ataaaaccga tctcacaagc aaaattgaat atatcgcgcc
      721 cggagaacat aaacagttac cacttccagc atctggcgga aagaacgtga aatgggctgc
      781 gatcaacgat tatggcggca gttccgggac agaaactcgt ccactgcaat aaaaaatata
      841 aaaacacagg ccatcaggga atgccacaac gacaccacca gggacataaa cgcacaccga
      901 aacagttggc gctcattatc aaacgttgtt tgccgatggt gctcactggc agcggcatgc
      961 tttgcactac cgctaacgcc gaagagtatt atttcgaccc cattatgctg gaaactacaa
     1021 aaagtggtat gcaaacaacc gatctgtcac gtttttcaaa aaaatacgca caactaccag
     1081 gaacttatca ggttgatatc tggctgaata aaaagaaggt ttcacagaaa aaaattacat
     1141 ttaccgccaa tgcagagcaa cttctgcagc cacagtttac ggtagaacaa ctacgtgagc
     1201 tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa agatgacgat agcgtgatca
     1261 actcgcttga acaaatcatt cccggtacag ctgctgaatt tgatttcaat catcagcgac
     1321 ttaatttgag cattccccaa attgcactgt accgtgatgc aagaggttac gtctcccctt
     1381 ctcgttggga cgatggtata ccaacgctgt ttaccaacta ctcgtttaca ggttctgata
     1441 accgttaccg ccagggcaat cgtagccaac gacagtacct gaatatgcaa aatggtgcta
     1501 attttggccc ctggcgatta cgtaactatt ctacgtggac acgcaacgat caggcgtcaa
     1561 gctggaacac tatcagtagt tatttacaac gtgatatcaa ggcgttgaag tctcagttgc
     1621 ttctgggaga aagcgccacc agcggcagta ttttttccag ctacgccttt actggcgtgc
     1681 aactcgcttc cgacgataat atgttgccaa acagccagcg cggatttgcc ccaacggtac
     1741 gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca aaatggttat gtgatctatc
     1801 aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct ctacccctct tccaacagcg
     1861 gcgatttaga agtcacgatt gaagaaagtg acggtacaca acgtcgcttt atccagcctt
     1921 attcttcatt acccatgatg cagcgacctg ggcatctaaa atatagcgcg accgctggac
     1981 gctatcgcgc tgatgcaaac agtgatagca aggaacccga atttgctgaa gccacggcaa
     2041 tatatggttt gaataatact tttacgctgt atggcggcct gctcggttct gaagattatt
     2101 atgcgctggg gatcggtatc ggcggcacac ttggcgcact gggcgcgttg tcgatggata
     2161 tcaacagagc tgacacccaa ttcgataacc agcactcttt tcatggctat caatggcgta
     2221 ctcagtacat caaagatatc ccggaaacca acaccaatat cgctgttagc tactatcgct
     2281 ataccaacga tggctatttt agttttgatg aagccaatac ccgcaattgg ggctataaca
     2341 gtcgccaaaa aagtgaaatt caattcaaca tcagccagac aatatttgat ggggtaagtc
     2401 tgtatgcctc cggttcacag caagactatt ggggcaataa cgagaaaaac agtaatatct
     2461 ctgttggggt ttccggccag caatggggaa ttggttacag cctgaattat caatacagcc
     2521 gctacactga tgaaaataat gaccgtgcac tctctttgaa tctcagtatt ccgttagaac
     2581 gctggtggat ttgcccctat atttccagac atctgttatc acttaacc
//
LOCUS       DAJTRH010000215         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTRH010000215 DAJTRH010000000
VERSION     DAJTRH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(64..822)
                     /locus_tag="OO339_004057"
     CDS             complement(64..822)
                     /locus_tag="OO339_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5928454.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO339_004058"
     CDS             complement(830..1933)
                     /locus_tag="OO339_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5928455.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OO339_004059"
     CDS             complement(1943..>2572)
                     /locus_tag="OO339_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5928456.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          682 c          601 g          578 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agctaggata aa
//
LOCUS       DAJTRH010000216         2562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTRH010000216 DAJTRH010000000
VERSION     DAJTRH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            133..562
                     /locus_tag="OO339_004060"
                     /pseudo
     CDS             133..562
                     /locus_tag="OO339_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            749..986
                     /locus_tag="OO339_004061"
                     /pseudo
     CDS             749..986
                     /locus_tag="OO339_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1407..1985
                     /locus_tag="OO339_004062"
     CDS             1407..1985
                     /locus_tag="OO339_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928457.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(2094..2352)
                     /locus_tag="OO339_004063"
                     /pseudo
     CDS             complement(2094..2352)
                     /locus_tag="OO339_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2336..2562
                     /locus_tag="OO339_004064"
                     /pseudo
     CDS             2336..2562
                     /locus_tag="OO339_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          775 a          471 c          660 g          656 t
ORIGIN      
        1 tcctgccggt atcaacgccg acggcatgta ttgccctgaa tgcggtacgg ttcactggcc
       61 ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt tcagcaggga
      121 aaccgatggc ttaactggct ggagaagcag ccggtgcaga tgagccgcct ggaacattat
      181 gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac
      241 cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg
      301 ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt
      361 gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta
      421 tcagcagtgt ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt
      481 tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc
      541 tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa
      601 cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct
      661 ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt
      721 aatcttttgg ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat
      781 actgacgtca aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc
      841 aacagcgatc ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa
      901 aagagaaacg taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa
      961 tggtggaaaa cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc
     1021 atcccctaaa cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt
     1081 atatctggcg aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa
     1141 tggaatggca gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga
     1201 acctgacgtg ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat
     1261 ggcatcctca tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca
     1321 gtaatgggga aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc
     1381 ctgttaatat ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt
     1441 ttcatgatat atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag
     1501 cagttttatc aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg
     1561 ccgaaaaaga caatgagata acaaagaaag acaaagccaa gataatagat acattaactg
     1621 aaaactattc cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat
     1681 ctttatatat tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa
     1741 tatgtcatac tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg
     1801 gtagcagcat tgacaaatat attacggata tgtgcggcct tatttgtatg aggcatattg
     1861 agtctactgg cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat
     1921 ttatgaatga aaaaggatgg cgggctgcgt tttgttcagc aaagaactta tataaatgtg
     1981 tttaatttta gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt
     2041 cagcagggcc tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg
     2101 tggtggttga ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag
     2161 aagcatatgg ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc
     2221 tctatccgct gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg
     2281 tataccggct caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag
     2341 agcagcgcgc atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt
     2401 ggcgaaacaa ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc
     2461 tgttgaaagt cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg
     2521 gatgaccgaa tccaggcagg aaaagctgat atggctgcgt gc
//
LOCUS       DAJTRH010000217         2551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTRH010000217 DAJTRH010000000
VERSION     DAJTRH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..546)
                     /locus_tag="OO339_004065"
     CDS             complement(<1..546)
                     /locus_tag="OO339_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928458.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..2197)
                     /locus_tag="OO339_004066"
     CDS             complement(1208..2197)
                     /locus_tag="OO339_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928459.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2281..>2551)
                     /locus_tag="OO339_004067"
     CDS             complement(2281..>2551)
                     /locus_tag="OO339_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928460.1"
                     /translation="LMTAYQRRQMNKRAEAERIAKELASQKERTREMFMTEDEWQKEL
                     QRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          664 a          590 c          614 g          683 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gagttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagcctac gacaaatcga agacaaccag ctaacctatt gtttcttaaa gacaggtgaa
      841 tccagttggt tgtagtgata gtcacacatt tagagataga aggtcaagac ttgaagcacg
      901 taatattccc tctaaaggaa tagaactcac ggtctgacta cccggccctt ttggtctggc
      961 tcaggtcagg ctatgggggc ttttggctcg ctctttatat tagatgaggt ctcacatttt
     1021 tcagtcaaaa ctcatcgtct aatagcccat agccagacca ccagtcccag ctttagcagg
     1081 aagaccacga gatagacttt gtgtaggtcc atttggccct cctactttct gtattcacaa
     1141 actagattac tagtacaaaa taaaggccag acatccagtt cggaagtctg accaatagtc
     1201 gttattgtta gttagcttct ttagtgaagc tcaggcgttt gactacaggc aggacagcag
     1261 tagccgagcc agtacccatc ttgatggtcg tagcagcttc agctacaaca aacaccttgc
     1321 cgagaatctc aaggtcgtga gactgggagt ctccatcacg tgaaaggacc acagtaatgc
     1381 tctcgccatc gtgacggaag caaccgaagc tcagagccgt aaaacgctta gccatcatga
     1441 acacgccacg gtcttcaccg ttgtaatagg cgacgtcagt ccccagcata tcgacgatag
     1501 cgtcttcacc accgaagccc tgcttagcag ccagacggga gagagccttg aggtgtgact
     1561 catgggcgat aatgccgtag tcagaaacct cagaaccgaa agaagactcc gtgttctcgt
     1621 acagggcatc catgatagcg tcagccttag cggtcaggct accagtcagg gccggaagtt
     1681 ctacggtctt cagcagcttt gaggactcca gcacgtcaac catcttagag ttaatggtac
     1741 gaatcacgtt atcgcagatg ttcagtgcgt cctgctggag cgtcccctga gtgaaaccag
     1801 tggtcgactt ctggtcccaa agctttgttt ggtgagacac gaggatcgcc tgttcaatcg
     1861 gctcaacggt gatttcagtg ccagacggag cggtcacaac ttcaggtcgc ttaccaccac
     1921 ggacagggac cagcacagag cggtcagcca gagtgtgagc aatgattgga gtcggtaagc
     1981 tgttacgggt cggcaggact tctacaggga acgggatgtt atcttgagcg tctttaagta
     2041 caccgttcag catagcctta accgtcttag ctaactggag ggcatagacc tgagggtcgg
     2101 cgttagtcgg cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct
     2161 caatgccaaa ctggaaagcg tttttagtca tattcattat aattaatctc cttgagtcaa
     2221 atacttgaag tccctaacag gcagtcaggg accgtgtgtt ctcagatgag ggacgcttgc
     2281 ttacaagctg tccatccagt taccgcctga ctcaaggtca gcgacgatag cggcacgtga
     2341 gcgaccagcg gaatgagtag cgctgcggag gttttcacca taggtccggc gttcagcttt
     2401 gacctgttcg cggcgctgta gctctttttg ccattcgtct tcggtcatga acatttcgcg
     2461 ggtacgctct ttctgagacg ctagctcttt agcgatacgc tcagcttcag ctcgtttgtt
     2521 catctggcgg cgttggtagg cggtcatcag g
//
LOCUS       DAJTRH010000218         2551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTRH010000218 DAJTRH010000000
VERSION     DAJTRH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.223"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..593
                     /locus_tag="OO339_004068"
     CDS             <1..593
                     /locus_tag="OO339_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5928461.1"
                     /translation="YGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEG
                     ECGLDMELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLT
                     GDVLNDDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDG
                     KHSWKSLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            615..1181
                     /gene="yieF"
                     /locus_tag="OO339_004069"
                     /gene_synonym="chrR"
     CDS             615..1181
                     /gene="yieF"
                     /locus_tag="OO339_004069"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5928462.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1235..2533)
                     /gene="adeP"
                     /locus_tag="OO339_004070"
     CDS             complement(1235..2533)
                     /gene="adeP"
                     /locus_tag="OO339_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5928463.1"
                     /translation="MLERVFKLREHGTTARTEVIAGFTTFLTMVYIVFVNPQILGVAG
                     MDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVVVQAMGLPWQIGMG
                     AIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMGLKNAGVIVANSET
                     LVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLGWMLGDVHYNGIVS
                     APPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVTDKAGLADEKGKFP
                     RMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAVVVGLLFLLVIFLS
                     PLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAVMMPFSFSITEGIA
                     LGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT          703 a          686 c          650 g          512 t
ORIGIN      
        1 tgtatggcat tggcgaattg ccggaaatcg tcaccctgcc gaaaggtaaa ccggttttca
       61 gtgataaaaa tttgccttcg ttttctattt cctatgccgg gaatatggtt ggcgtggcgt
      121 taacaaccga aggtgaatgt ggcctcgata tggaactaca gcgtgcgacg cgcgggtttc
      181 atagcccaca cgcgcccgat aaccacacct tttccagcaa tgaatcgcta tggatcagta
      241 aacaaaacga tcctaacgaa gcgcgggcgc agctcattac gctgcgccga agcgtgctaa
      301 aactaaccgg tgatgttttg aatgacgatc cgcgcgatct gcagctgctg cccattgccg
      361 gacgcctgaa atgtgctcat gtaaatcatg tagaagcgtt atgcgacgcg gaggacgtgc
      421 tggtgtggtc cgtggcggtc acgcccgcga ttgaaaagct cagtgtctgg gagttagatg
      481 gcaaacacag ctggaaaagc ctgccggata ttcacagccg cgccaacaat cctaccagcc
      541 ggatgatgcg ttttgcccag ctctctaccg tgaagtcttt ttcgccaaat tgatagacaa
      601 ccacaggagt cattatgtct gaaaaattgc aggtggttac gttactgggg agcctgcgca
      661 aaggctcatt taatggcatg gttgcacgta ccctgccgaa aattgctccg gcgagcatgg
      721 aagtcaatgc gttaccttcc attgccgaca ttcccctgta tgacgctgac gtacagcagg
      781 aagaaggttt tccagcaacg gttgaagctc tggcggaaca gatccgtcag gctgacggtg
      841 tggtgatcgt cacgccggaa tataactact cggtaccggg tgggctgaaa aatgccatcg
      901 actggctttc ccgcctgccg gatcaaccgc tggccggtaa accggtgttg attcagacca
      961 gctcaatggg cgtgattggc ggcgcgcgct gtcagtatca cctgcgccag attctggttt
     1021 tcctcgatgc aatggtgatg aacaagccgg aatttatggg cggcgtgatt cagaacaaag
     1081 ttgatccgca aaccggagaa gtgattgatc agggtacgct ggaccacctg accgggcaat
     1141 tgaccgcatt tggtgagttt attcagcgag ttaagatcta aataaaaaac ccgccagtaa
     1201 tcatgcatgg cgggttttta atgcgctatc gattttaatg agcgtcgata aatacaatct
     1261 tcaggataaa cagcagcgca acgatgatta cgcacgggct aaggtcacgc agacgcccgg
     1321 taccaatctt catcacgcag taggagataa agcccagcgc aataccttcg gtaatcgaaa
     1381 agctgaacgg catcatcacg gcggtaataa acgccggaac agattcagta agatcctgcc
     1441 agttcacgcg agcaagactt gaggtcatca acacgccaac gtagatcaac gcgccagctg
     1501 cagcgtagtc tggcaccatt cccgccagcg gcgacagaaa gataaccagc aggaacagca
     1561 gaccaacaac cactgccgtc agaccggtac gaccgccaac cgatacgccg gaagaggact
     1621 caatataagc cgtaacggaa gaagtaccga taaacgaacc ggtcacggaa gagatactgt
     1681 cgacatacag cgcctgcttc atgcgcggga atttcccctt ctcatccgcc agacctgctt
     1741 tatcggtcac gccaatcagc gtaccggagg agtcaaacaa gttgaccaac atgaaagaga
     1801 aaatcacccc tgccagcccg aggttaaacg acccggctaa atctacatga cccacaactg
     1861 tcattacgct cggcggcgca gaaacgatgc cattgtagtg cacatcaccc agcatccagc
     1921 ccagcagcgt cgtcaccacg atagaaacca gcaccgctgc gtgaatgttg cgcgaggcca
     1981 gaatagcaat gatgaaaaag ccgaggatac ccagaagtac gctgtgagaa gtcagattac
     2041 cgatgctcac cagcgtttcc gagttagcga caatcacacc tgcgtttttc agccccatca
     2101 tgccgataaa caggccgata ccgctggtaa tacccacacg cagacttacc ggaatgttag
     2161 caatcatcca gtaacgaacg cggaaaatgg tcaaaagaag cagtccgaca gcgccccaga
     2221 agattgcgcc cataccgatc tgccacggca gccccattgc ctggacaacc acaaaagcga
     2281 aaaaggcgtt aagccccatc gcaggagcca gcgccaccgg aaggtttgca aacaatccca
     2341 tcataatgct accgaaagca gcaatcagac aggttgttac gaagacggca ctggtgtcca
     2401 tgccagcaac gcccagaatt tgtgggttaa caaatacgat gtagaccatc gtcaggaagg
     2461 tggtgaaccc ggcaatcact tcagttcgtg ccgtagtacc atgttcgcgc agtttaaaaa
     2521 cacgttccag catcctctga ccagaagtct g
//
LOCUS       DAJTRH010000219         2532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTRH010000219 DAJTRH010000000
VERSION     DAJTRH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            131..1999
                     /gene="kup"
                     /locus_tag="OO339_004071"
     CDS             131..1999
                     /gene="kup"
                     /locus_tag="OO339_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5928464.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2166..>2532
                     /gene="rbsD"
                     /locus_tag="OO339_004072"
     CDS             2166..>2532
                     /gene="rbsD"
                     /locus_tag="OO339_004072"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5928465.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRS"
BASE COUNT          557 a          604 c          660 g          711 t
ORIGIN      
        1 tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc cgttaatcgt
       61 gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata tttcaacgaa
      121 agactagtct atgagcactg ataataagca atcattgccc gcgattaccc tcgcggcgat
      181 tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg aatgtttgtc
      241 cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat cgctgatctt
      301 ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc gggcagataa
      361 cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata cgtcggcgcg
      421 aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct atggtgaagt
      481 cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa tcgtcgcccc
      541 gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat tatttatgat
      601 tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc tgacctggtt
      661 tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag tgctgcatgc
      721 gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt cttttattgc
      781 attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg atatggggca
      841 ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt ccttgaccct
      901 taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta agaacccgtt
      961 cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg cactggcgac
     1021 ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc aggcggtacg
     1081 tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt cagggcaaat
     1141 ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga ttgtcagctt
     1201 tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa ccatggtact
     1261 gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata agtattttgt
     1321 tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg ctaacctcga
     1381 taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt ttatcgtgat
     1441 gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac atggtaactc
     1501 tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc ccgggaccgc
     1561 ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata accttaaaca
     1621 taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag acgcgccata
     1681 tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct ggcgcgtggt
     1741 ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc gctgcggtct
     1801 ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg agtcgttgat
     1861 cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc tgctgcaacg
     1921 taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta tcgaactggg
     1981 tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc gtttttgttt
     2041 tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta acgtcacgat
     2101 ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg ctgatggaga
     2161 aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc tcccgtctgg
     2221 gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa agtacaacgc
     2281 gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg ggcgtcgtca
     2341 caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa cataatccgc
     2401 aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag ggaaatacca
     2461 ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa agtcaggcgg
     2521 taattcgcag cg
//
LOCUS       DAJTRH010000220         2531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTRH010000220 DAJTRH010000000
VERSION     DAJTRH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            274..578
                     /locus_tag="OO339_004073"
                     /pseudo
     CDS             274..578
                     /locus_tag="OO339_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            747..2111
                     /locus_tag="OO339_004074"
     CDS             747..2111
                     /locus_tag="OO339_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5928466.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            2161..2370
                     /locus_tag="OO339_004075"
                     /pseudo
     CDS             2161..2370
                     /locus_tag="OO339_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2452..2531)
                     /locus_tag="OO339_004076"
                     /pseudo
     CDS             complement(2452..2531)
                     /locus_tag="OO339_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          655 a          504 c          587 g          785 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1981 tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc gtactgatac
     2041 tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca ctggagttcg
     2101 aaaaagagta attttttcag gaaaaacata aaattactga ctaaatattt attttttgaa
     2161 tcgcagggca ttccggataa ggttgatatc gtgtcacggc tgaagaccta tggatacagt
     2221 atttctggtg tggagacgga tgacggatat aaggcgcttg tccgcgcttt tcagcttcat
     2281 ttccggcaga aaaattatga cggcataatg gatgctgaaa ccgctgccat cctgtatgca
     2341 ttgctggaga agtatttccc gggtaaataa ggagtgagtc gtcagagctg cgatatatcc
     2401 ccttcctcca tgggctgtct cttatacaca aatccccacc cggggatttg tgatgtcaga
     2461 tgtcccctgt aaaactatgt tttcattgcg ctctatgaag ctgagtgagc gtaacgactg
     2521 gagctgcttc t
//
LOCUS       DAJTRH010000221         2423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTRH010000221 DAJTRH010000000
VERSION     DAJTRH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..98
                     /locus_tag="OO339_004077"
     CDS             <1..98
                     /locus_tag="OO339_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5928467.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="OO339_004078"
     CDS             156..512
                     /locus_tag="OO339_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928468.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="OO339_004079"
     CDS             605..823
                     /locus_tag="OO339_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5928469.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="OO339_004080"
     CDS             825..1190
                     /locus_tag="OO339_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5928470.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="OO339_004081"
     CDS             1187..1852
                     /locus_tag="OO339_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928471.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="OO339_004082"
     CDS             1852..2217
                     /locus_tag="OO339_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5928472.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          684 a          490 c          647 g          602 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctg
//
LOCUS       DAJTRH010000222         2419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTRH010000222 DAJTRH010000000
VERSION     DAJTRH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            236..1849
                     /gene="mppA"
                     /locus_tag="OO339_004083"
     CDS             236..1849
                     /gene="mppA"
                     /locus_tag="OO339_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5928473.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1900..>2419)
                     /gene="ynaI"
                     /locus_tag="OO339_004084"
     CDS             complement(1900..>2419)
                     /gene="ynaI"
                     /locus_tag="OO339_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5928474.1"
                     /translation="MLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLY
                     VPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQT
                     LLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTL
                     YMDNITPPEQGR"
BASE COUNT          669 a          537 c          591 g          622 t
ORIGIN      
        1 ctgattatga taccgaaaac tggtttgaac gtgcgaagcc cgaacgtagt gttcggtgtt
       61 ctatgtgctt tgccacattt tggcgtctat tatttacact aaatctgatt tgatatattg
      121 atacttaaaa catttgatgc ttcctttgtc acttttttga tggaagttgt ttgcatttct
      181 ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga aacttatgaa
      241 gcactctgtt tcagttacgt gttgtgcgct gttggtcagc agcatttctc tttcgtatgc
      301 tgcagaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc gccacattaa
      361 agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga ttcaggtcat
      421 tcgcgatctg tttgaaggtc tggtgaatca gaacgaaaaa ggggagattg tccccggcgt
      481 cgcgactcaa tggaaaagta atgacaaccg tatctggact tttaccctgc gcgataacgc
      541 aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct ggcaacgtct
      601 ggtggaccca aaaacattgt cgccatttgc atggtttgcc gcgctggcgg gaatcaacaa
      661 cgcacaggcg attattgatg gtaaagctac gcctgatcag cttggcgtca ccgcagttga
      721 tgcccatact ttgaaaattc agcttgataa accgttgccg tggtttgtga atttaaccgc
      781 taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcggtaaag agtggacgaa
      841 acccggaaat ctgatcggca atggcgctta tgttcttaaa gagcgcgtag tcaatgaaaa
      901 actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtgc tgcaaaaagt
      961 gaccttcctg ccaattaatc aggaatccgc agccactaag cgttacctcg cgggggatat
     1021 tgatatcacc gaatccttcc cgaaaaatat gtatcagaag ctgttgaagg atattccggg
     1081 gcaggtttat acgccaccgc agctcgggac ctattattat gcctttaaca cgcaaaaagg
     1141 gccgacggca gatcagcgcg ttcgcctggc attaagtatg acgatagatc gccgcctgat
     1201 gaccgaaaaa gtgttgggga cgggcgaaaa gccagcgtgg cattttatac cagatgttac
     1261 cgccggattt acgccggaac cttcgccgtt tgaacaaatg agtcaggaag aactgaatgc
     1321 gcaggcaaaa actttgttga gcgcagctgg ttatggtccg caaaaaccgc tgaagctgac
     1381 gcttttgtat aacacttcag aaaaccatca aaaaattgcg attgctgtag catcgatgtg
     1441 gaaaaagaac cttggcgtag atgttaaatt gcaaaatcag gaatggaaaa cctatatcga
     1501 tagccgtaac accggcaatt ttgatgttat ccgcgcctca tgggtggggg attataatga
     1561 accctccact ttcctgacat tattaacgtc aatgcattca ggaaatattt cacgctttaa
     1621 caatccggca tacgacaaag ttctggccca ggcatcgacg gaaaataccg ttaaagcgcg
     1681 taatgccgat tacaacgcgg cagaaaaaat cctcatggag caagcaccga ttgcaccaat
     1741 ttatcaatat accaatggac gattaatcaa accgtggctg aaaggttatc ccattaataa
     1801 ccctgaagat gtagcgtaca gtcggactat gtatattgtg aagcattgat gtgatgggaa
     1861 ctggcgttac ccttgtggat aacgccagtg atgtctgatt tagcgcccct gttccggcgg
     1921 tgtaatgtta tccatataca gcgtctggct ggggaaggca aagtccgcgc cgtgtgactg
     1981 tacaatatcg ataatcttca aataaacgtc ttgctgtgca gcaagccact cagcccatac
     2041 cgtggttttg gtaaagcaat aaaccataat attcaatgac gagtcagcaa actggttgaa
     2101 ataaaccagt aaggtttgtc gctggtcgat ggccgggtga tttttcagca tctcacgtac
     2161 ggcttcgaca ataacgccca cttttgccgc atcctcataa cgtaaaccaa tggtcgtggt
     2221 aatgcggcgg ttggtcattc gtcctgggtt ttctacgctg atcgacgaaa acagcgagtt
     2281 cggtacgtac aatggacgat tatcaaaggt cgtaattttg gtaattcgcc agccaatttc
     2341 cgctactgta ccttcgatat ttctgtccgg tgaacggatc cagtcgccaa tactgaaagg
     2401 acggtcgaaa tagagcatg
//
LOCUS       DAJTRH010000223         2310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTRH010000223 DAJTRH010000000
VERSION     DAJTRH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            53..>2310
                     /locus_tag="OO339_004085"
     CDS             53..>2310
                     /locus_tag="OO339_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5928475.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHV"
BASE COUNT          616 a          511 c          583 g          600 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccctcaat gagtgggcat cgcggcgcaa cagccctgaa gacttttggg aaacaggtaa
     1501 ccgacgtagt cgcgtggagg gaacgctaac gcagtcgttg gggagagatt atggcaattt
     1561 atacctgaca ttaagccggc aacaatactg gcataccgat gatgtcgaac gattaatgca
     1621 atttggctac agcagtagct ggaagcgtct ctcgtggaac gtctcctgga gttattccaa
     1681 tactgccaga caggggacgg ggaacaacca tgccagtgat aacaccagtg agcagatcta
     1741 catgctctct ttatctgttc ctttatcggg ctggtggggt aatagttacg ccacctattc
     1801 tgtttcgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg gtacggcgct
     1861 ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg atgatgaggt
     1921 tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg gcagctataa
     1981 ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc tggcacacag
     2041 cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta aagcacctgg
     2101 ggccgccagg ttagaaatag ataatatgcg cggtgctgcg acggactggc gcggctatac
     2161 ggtcaagaca cagctaaacc cttatgatga aaatcgggta gcaatcagcg ataactattt
     2221 ctcgaagtcg aatatagaac ttgataatac cgtcgttacg atggttccca cgcgtggtgc
     2281 agtggttaaa gcggagtttg tgactcatgt
//
LOCUS       DAJTRH010000224         2282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTRH010000224 DAJTRH010000000
VERSION     DAJTRH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..799
                     /locus_tag="OO339_004086"
                     /pseudo
     CDS             1..799
                     /locus_tag="OO339_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="OO339_004087"
                     /pseudo
     CDS             914..1255
                     /locus_tag="OO339_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="OO339_004088"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="OO339_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2125..>2282)
                     /locus_tag="OO339_004089"
     CDS             complement(2125..>2282)
                     /locus_tag="OO339_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5928476.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT          553 a          555 c          692 g          482 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaatgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc ctggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc ttaggggggt
     2101 gcccctatta agttgtcaag catgttatga cccctgcggg gtataaaaag tcccgtcgcg
     2161 catcatggcg aacagaacgt cgcagcgtcg tctcgccagg gcgataagcg cctgattgtg
     2221 tcgttttccc tgactcattt tgcgggtgta gtaagccctg gagagcggat ccctgagcgc
     2281 gg
//
LOCUS       DAJTRH010000225         2251 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTRH010000225 DAJTRH010000000
VERSION     DAJTRH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..252
                     /locus_tag="OO339_004090"
     CDS             <1..252
                     /locus_tag="OO339_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5928477.1"
                     /translation="RKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNA
                     EMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            210..887
                     /gene="bglJ"
                     /locus_tag="OO339_004091"
     CDS             210..887
                     /gene="bglJ"
                     /locus_tag="OO339_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5928478.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(925..1713)
                     /gene="fhuF"
                     /locus_tag="OO339_004092"
     CDS             complement(925..1713)
                     /gene="fhuF"
                     /locus_tag="OO339_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5928479.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            1854..2090
                     /locus_tag="OO339_004093"
     CDS             1854..2090
                     /locus_tag="OO339_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5928480.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2129..2215)
                     /locus_tag="OO339_004094"
     tRNA            complement(2129..2215)
                     /locus_tag="OO339_004094"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2179..2181),aa:Leu,seq:cag)
BASE COUNT          582 a          494 c          587 g          588 t
ORIGIN      
        1 cgcaaggtac tgcggctatt aggtaaagga tggggcatca accagatagc ttcattgctt
       61 aagaaaagta ataaaactat cagcgcccaa aaaaacagtg cgatgcgtcg actggcaatt
      121 cacagcaacg ctgaaatgta tgcatggata aatagcgcgc agggtgcaag agaacttaac
      181 ttgccttctg tttatggaga tgccgcagaa tggaacacag ccgaattaag aagagaaatg
      241 tcgcactcat agaaaaatgc gtcatgagta gtatcggtat tgagagttta ttcagaaagt
      301 ttgcgggcaa cccttataag ctccatacct ataccagtca ggagtcattt caggatgcca
      361 tgtcgcggat ctcgtttgcg gcggtcattt tttctttttc tgccatgaga agtgagcgca
      421 gagagggatt atcttgcctg actgagctgg cgattaagtt tccgcgtacc cggcgtttag
      481 ttattgtgga tgatgatatt gaagctcggc tgattggttc attgtcgcca tcaccgctgg
      541 acggtgtatt aagtaaagcg tcaacgctgg agatttttca tcaggaactc tttttgtcat
      601 taaatggtgt acgtcaggcg accgaccgac tgaacaatca gtggtacatt aaccaaagcc
      661 ggacgttaag cccgacggag agagaaatat tgcgctttat gtcgcgtggc tactcaatga
      721 cacaaattgc cgagcagctt aaacgtaata tcaaaacgat ccgagcgcat aaatttaatg
      781 tgatgtcgaa actgggcgtc agttctgatg cagggttgtt ggaggccgaa gatattctgt
      841 tatgtatgcg gcattgcgaa gcaagtaatg tgttgcatcc ctattaatcc gcatgatgcc
      901 gggtttactt ccccggcagt gctttcattt cagcgtacaa tagccacatt gctgcacatc
      961 cggtaagcga taacgctggc agcaagtgcg gcgcaccagc aggccgtcgc gcagtaccac
     1021 ggtacgccag agtggattat cttcaccgtt cgtgagcgtt ttctcaaaaa agagggcatg
     1081 gcgcagcgat tcaacagtag cctcgccgag cagttgcttc atctcagtga gataccagtt
     1141 gatcaaataa ccggtattac tccagataag tttgccgttg atctctccgg tcgcttctag
     1201 tgcttgcaca accggaacca gcgcctggct gattaacgtt tccattcgct gctgcggcga
     1261 atgtggtgtt gcgtttttat cttcacacac atcgacccag aaacaggcga cgcgtccggt
     1321 ttcgtgaaac tcagcatgga aatgttccgg cgacacatct aatgcttttt cctgcgtcag
     1381 tagcgccagc attaatggtg gcaccatcag gccgatatac cattgtgccc atagtgagat
     1441 cagcggtttg ttctcgcgga tcatcgttgg ttggttgcga tagatatgat cggaatagac
     1501 cgccagtaga gaacttagcg cattcggtga tgaccattgc gccagcgtca tggcgttaag
     1561 cggagcgggt tcatccaggc ggataaactc cagcaaatgt tcacgatgtt tggcgatcat
     1621 cgcccgtacg gcttgtgcaa gcccagcatc ctgcggctgg agatgcgttc gccagatgac
     1681 atcttcatag agcggtgcgg aacgataggc cataatcgtg atagtaatct aaatgataat
     1741 gattgctaat cctagcgata ggtttactcg atagcaaggg atttctctgg cttgcaaatg
     1801 ataaaaatta tcatatgata ttggttttca ttatcaatga aagagatgaa accatgttgc
     1861 aacgtacgct gggcagtggc tggggagtgt tgctgccggg attgctgatt gcagggctga
     1921 tgtatgcgga tttatcgccg gatcagtggc ggattgtcat tctgatggga ttagtattga
     1981 cgccggtgat gctgtatcac aaacagttgc ggcattacat tttgctacca tcgtgcctgg
     2041 cgcttattgc tggcatcatg ctgatgataa tgaatttgaa tcagggatga caaaacaggg
     2101 aagaaacaag aaaggaagta aagataattg gtgcgagggg ggggacttga acccccacgt
     2161 ccgtaaggac actaacacct gaagctagcg cgtctaccaa ttccgccacc ttcgcacagt
     2221 catcttactt tttttgatat cgcctcgttt g
//
LOCUS       DAJTRH010000226         2248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTRH010000226 DAJTRH010000000
VERSION     DAJTRH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            661..2169
                     /gene="ltrA"
                     /locus_tag="OO339_004095"
     CDS             661..2169
                     /gene="ltrA"
                     /locus_tag="OO339_004095"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5928481.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          573 a          555 c          664 g          456 t
ORIGIN      
        1 gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt
       61 aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca
      121 gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga
      181 tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt
      241 taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca
      301 ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga
      361 aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa
      421 gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac
      481 cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg
      541 agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga
      601 atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa
      661 atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg
      721 ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca
      781 aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg
      841 gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc
      901 gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg
      961 ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt
     1021 gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc
     1081 acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc
     1141 gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc
     1201 aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc
     1261 gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg
     1321 ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc
     1381 gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg
     1441 gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat
     1501 tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg
     1561 ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat
     1621 gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc
     1681 gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg
     1741 acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac
     1801 cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc
     1861 agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt
     1921 acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa
     1981 acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg
     2041 ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg
     2101 aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc
     2161 agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg
     2221 cagggaaata gtccgactac gccctgcc
//
LOCUS       DAJTRH010000227         2215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTRH010000227 DAJTRH010000000
VERSION     DAJTRH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            84..707
                     /locus_tag="OO339_004096"
     CDS             84..707
                     /locus_tag="OO339_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5928482.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
     gene            complement(761..1933)
                     /locus_tag="OO339_004097"
     CDS             complement(761..1933)
                     /locus_tag="OO339_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5928483.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
BASE COUNT          681 a          395 c          453 g          686 t
ORIGIN      
        1 cttgtgaagg aaatggtact cagggggatt ccagtacagg tggtgcgcaa ttagctataa
       61 atggccttaa cggttcgtgc gctttggtat taaacaaccc atactttgag gctaatgccg
      121 ggggggcaga ccttgctata gataatactg ggacgcgccc tgtaactgtg gttataaacg
      181 gtggaaattt tcatcgcgtg agcagtgcaa gatacacgca cacgaatatt caagtaacaa
      241 gctcaggtgg gggaaaagtt acagtcatac tcaacggcac aacgttccag agcgcagggg
      301 attatcagcc aagtgcagaa cgaccatact ggataacagg agctaattgc gaggtagttg
      361 acattgggtg tgtttttaca gaaacaacca gtaaggtaac atcggcttct gcattgtcag
      421 tcacacgctc gggaaaaatt aacgccaatg gtagtatcga cgttgctact ggtgtttcat
      481 cggttaacgt ggtttctaca ggtgtgtatg atgttagctt ttctcatccg ctcgcagcag
      541 ttgctagcgg ttatattgtg cagataacac ctatatctgc gcctgattca gttagttgtg
      601 atgttactta tattggcgta gatacattta gggtgaccct gcgcaatacg cttagcgggg
      661 caggaatctc cagttcgttt gcatttagca ttaccagact aatataaata cattcgccgc
      721 cccatttgga catatatgta gggcggctta tgtttattgt ttattgattg ttgtttgtta
      781 atgtgggtat taattctttt actaaagttg tggtttgttt tgttaatttg atgaatggcg
      841 tctctattaa tttaaaagtt aggcatgatg tgaacgtaat aagaaatgca gttgtgctta
      901 ccagtatgat aaatgtattt tcagtgtaat tattaggaat aatccacgtc attaggcaat
      961 aaaggaatat cccatgcaga aggtaaacgc tgtaggtagt ttctcctagc tttctggtta
     1021 tatttaatct taatattcca tacaaatcac aacctgatgc gataatgata aatgttattc
     1081 cacaaagaat cagtggtagc ggtgcgtaag ttgttttgaa atacgtcatt tcaaaaacca
     1141 ttatcgcagt tattataatt ggtgttactt ttgctttggc aattccattg actatctttg
     1201 atttatttag caaaaatgcc aggagcccaa aaagaaatga tgctatgtgt attttactga
     1261 aaaataaaat gaaaacaaat agcgatattg cgctaatcat aaccatgtaa atactgactt
     1321 ttctttttat gagcgcggaa attaccggaa gagaaaaata aaagaaccat tcatatacaa
     1381 gtgtccatgt tacagcggca ttgatagtaa atgaatcttt tacgtcatta atgttcggca
     1441 tacctagcgc agtgaatggc aaccacttca ttattgacac aaaaagctct tctgtggata
     1501 cctgcattct ccatccagac ttaaaaccta caatgataaa aatgagacat aggctaacta
     1561 tgaacattgg ggttaatcgt agtaatcttg atacataaag ccttgtccag tcctgatctc
     1621 cagagataat ctttgaaaag aacagataac cagtaatcat aaaaaagaat gacacaccaa
     1681 cttggcctaa atttgctaac agatttgatg aaggtgcttc ccatactcca gatgacaagt
     1741 atccattcca aattgctgcg tgatgaataa gcacgaaaat tgctaagaac ccccgcattc
     1801 catctatttg attattcctg ccgtgctcaa caagatttat tggggaaatt ttatcaaata
     1861 accccacagc taataacgac atcaatatca cggatgttat taataataaa ctgttaattt
     1921 caatcatatg catgctctat tacgcaatta aaatttctcc aagtatactg acttttagtt
     1981 aatgatcata tagttatgtg taactgatga aacaaaacgg agacacacca agctttgcac
     2041 tggattgcaa ggctttgtgc tcttctgtga atgtgtggct atatgtttga agatcgttgt
     2101 gccgtatttg tgacatatat atggcaacat catgcatcaa ctttctgttt gtgtcatcaa
     2161 ctatagctta gtgaatgcgg ttaatgcttg ctaaaacaga tagttatgat tggtg
//
LOCUS       DAJTRH010000228         2204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTRH010000228 DAJTRH010000000
VERSION     DAJTRH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..161
                     /locus_tag="OO339_004098"
     CDS             <1..161
                     /locus_tag="OO339_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5928484.1"
                     /translation="TKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGA
                     NTPFIQVK"
     gene            205..954
                     /locus_tag="OO339_004099"
     CDS             205..954
                     /locus_tag="OO339_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5928485.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            951..1520
                     /locus_tag="OO339_004100"
     CDS             951..1520
                     /locus_tag="OO339_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928486.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            1644..1886
                     /locus_tag="OO339_004101"
     CDS             1644..1886
                     /locus_tag="OO339_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5928487.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            1890..2036
                     /locus_tag="OO339_004102"
     CDS             1890..2036
                     /locus_tag="OO339_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928488.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            2045..>2204
                     /gene="xisR"
                     /locus_tag="OO339_004103"
     CDS             2045..>2204
                     /gene="xisR"
                     /locus_tag="OO339_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5928489.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          592 a          524 c          584 g          504 t
ORIGIN      
        1 gcacaaaagc ccaggccgaa gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga
       61 acccaaacgt tatggagctg gaagccatca acaagtggaa cggtacactg ccgcaataca
      121 tgaccagcgg tgccaacaca ccatttattc aggttaagta atacatatgc ccggtattac
      181 acgccgggct gtctggagat aaaaatgaat actgtaacca tcaacaacaa acagtttccg
      241 gtaatcgaat atcgcggtca gcgtgttgtg acgctggcga tgattgatga agtccaccag
      301 cgaccggaag gaaccgcgcg tgcagcattc aaccgaaacc gtgagcattt catcagcggt
      361 gtggattatg ccgaattagg tgcggacgta atacgtacgg acctcccgga aggaacattc
      421 tctaaatttg caccatcggg gattgtactt tttgaatcag gttacctgat gttgacgaag
      481 ccattcaatg acgcccttgc ctggcaggtt cagcgcgaac tggttaacag ctacttccgc
      541 actcgcacac cgctaacgga aatcgagatg atcgctgcga tagccgccga cgctgttcgt
      601 caacagaagc gcctgaatca tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa
      661 accatcaaac gcggaaatat gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa
      721 agtggaatga gtgacaccaa gtgtcggaat ctggtgaacg catatcaaat tcctacagac
      781 acccacgaat tcatgactcc ggacggtctg ttgtctcgca gggctatcgt cgagcttgag
      841 ccatttatgg cggcgttcca ccaaatgatg tcagaggctg acccacgcgg cacacgctgg
      901 tatcacccga agatggggct tttccaggtg atcggatggg aggataaagc atgatcatcc
      961 agtcaaaact tattcgcgcc gctctggtgt gcgctgctaa aaacgacgtt cgttattacc
     1021 tgaacggtct tcacatcacg ccgaaacata ttgaggcaac caatggttcc gtggcactgc
     1081 gcatggctca cggcatccgg acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc
     1141 cggccaaagc cgaaacgaca gagctgattt ttagtaaaga gccgatcgct gttcatcgcg
     1201 accagtttca gcgacgactg tccattaccg gcattaaact ggtggacggt tgttttccgg
     1261 atttggatcg catcattccg aaaaagtttg accgctgtac tcacccggtg ctccaggcgg
     1321 gttacctgag ttatccagag aagatgtttg gtcgtgagcg taaatttatt cccgtccagt
     1381 tacgtccctc cggtgacggg caagcggtca gaattcagtt tgattccatc atcaactcaa
     1441 tgtatggcaa tcctgaattt gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg
     1501 ctcaggagca tccggaatga aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag
     1561 ctggcctgat gcaatcgtaa ctctggggat ggtattcgca gtagcgtttg ttgtgtactc
     1621 gatttgtcga tggggataac cacatgttcg ctttgattca acgcgggcag atatacacag
     1681 acagagccgg atacccagta gaaattactc gcagtactga gcactcagtg ttctttcgga
     1741 ggatggacgg acgtaccggg cgagtacgca tcggtgaatt cagtagcctg ttcgagcata
     1801 ttgaccacct ggaatatcac aagattctgg ctgaaacaga acaagaaaag cacctgaaga
     1861 aattacgcgc aatgcaaagg aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc
     1921 agcgttacgg caaccactac gacctgacgc gagatgttga cggtttctac tgtcgtgaaa
     1981 ttgtgaaacg aatgtttgag gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta
     2041 atgcatgggc aatgtgattc aactggctcc caatgaatgg gtttgtgaaa gcgttctaat
     2101 cgcaattacc gggctcaaac caggcacaat tcttcggacc cggaaagaat gctggatggt
     2161 tggaagagag tatattcacg tatcaccaga cggtaatcca aagc
//
LOCUS       DAJTRH010000229         2144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTRH010000229 DAJTRH010000000
VERSION     DAJTRH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(302..628)
                     /locus_tag="OO339_004104"
     CDS             complement(302..628)
                     /locus_tag="OO339_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5928490.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            763..1104
                     /locus_tag="OO339_004105"
     CDS             763..1104
                     /locus_tag="OO339_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5928491.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1170..1699
                     /gene="speG"
                     /locus_tag="OO339_004106"
                     /pseudo
     CDS             1170..1699
                     /gene="speG"
                     /locus_tag="OO339_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1702..>2144)
                     /locus_tag="OO339_004107"
     CDS             complement(1702..>2144)
                     /locus_tag="OO339_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5928492.1"
                     /translation="VLDANYYRDAETLEKRVRLQGKCQLAELPSAGVSWETDDNGFVI
                     KASSKQMQMEYRYDDQGYPLGKTTKSNDKTLSVSATPSTDPIKKLDYTAVTLLNNQRV
                     GNVKQSCEYDSHANPVDCQLIIVDEGVKPAVERVYTIKNTIDYY"
BASE COUNT          606 a          521 c          462 g          555 t
ORIGIN      
        1 cccaaggccc gtaataccgt cctcagtggt gatttttaat gtgacaaaat tacgccccgg
       61 acaggtaaca aaaacttcag cctttacgat cttcatgttc gattccttgc atcgcttgtc
      121 gtgatgcatg aaatctacgc aactgagcta ctgccataca agtatgaaga tcgaaaaaag
      181 ccggagtgat cacaaaaaaa ggcgtatatt tgcgctgtga atggttgaca aaagatgaaa
      241 tagaatacct tttgtcagct gacacttcct cgtatcttat tgataaaatg gatttatgtt
      301 cctacgtgcg cccccagccc gcaacaatga tcaacattcc gcaaagcgca atcaacgcac
      361 ccgtccagtc ataaagactt agtttcacgc catccacaac gcgcagccac atcaatgccg
      421 tgcagacata aacgccacca taagccgcgt aaacacgccc actcgccgca ggatgcaacg
      481 ttaacaacca gacaaacagc gccagtgaaa tccccgccgg aagcaacagc cagatactgg
      541 cgtttcgttt taaccacaac cagggcagaa agcatccaat aatttcacac agcgcagtag
      601 caaaaaatag taacgttgtt ttaatcatct ttgtcactta ttgacatcat gtatagttat
      661 agggcgacat aatatcatca atataaacat cctcctggta cgttttgcgt tcgcagtgga
      721 tggtgtagaa tcacctttaa tcattcatac agggaatgaa ttatgaaaat cactctcagc
      781 aaacgaatcg gcctgctcgc ttttctgctg ccttgcgcac tggcattgag cacaactgtt
      841 catgccgaaa ctaacaaact ggtgattgag tctggcgaca gtgcacaaag ccgccagcac
      901 gccgctatgg aaaaagagca atggaatgac acgcgcaatc tgcgccagaa agtgaataaa
      961 cgcactgaaa aagagtggga taaagccgac gccgcttttg ataaccgcga taaatgtgag
     1021 caaagtgcca acatcaatgc ctactgggag cccaatactt tgcgctgcct ggaccgtcga
     1081 actggccgcg ttattacccc ctaacctgtt attgatttaa ggaatgtaag gacacgttat
     1141 gccaagcgcc cacagttaag ctacgcccgc tggagcgtga agatttacgc tatgtacatc
     1201 aactcgacaa taacgccagt gtgatgcgtt actggtttga ggaaccctac gaagcctttg
     1261 ttgaactctc tgatctgtat gataagcgca tattcacgat cagagcgaac ggcgctttgt
     1321 ggtggaatgt gacggcgaaa aagccggtct ggtggagctg gtggaaatta accatgttca
     1381 tcgccgggca gaatttcaga taattatctc cccggagtat caggggaaag gtctggcaac
     1441 ccgtgccgcc aaattagcaa tggactatgg cttcaccgtt ctcaatctct ataagctgta
     1501 tctgatcgtc gataaagaga atgaaaaagc gattcacatt taccgcaagc ttggctttac
     1561 ggttgaaggt gaattgatgc atgagttttt tattaatggt caatatcgta atgccattcg
     1621 catgtgtata ttccagcatc agtatctggc agagcacaaa acaccgggtc agactctcct
     1681 gaagccgacc gcacaatagc attaataata atcgatcgta tttttgatgg tgtaaacccg
     1741 ttcgacggcg ggttttactc cttcatcaac aatgatcagc tgacagtcca ctggattggc
     1801 gtgactgtca tattcacagc tctgttttac attaccaact cgttgattat ttagtaaagt
     1861 aaccgctgtg taatctaatt ttttgatcgg atccgttgat ggcgtggcgc tgacagataa
     1921 tgttttgtcg ttacttttcg tggttttacc cagcggatac ccctgatcat catagcgata
     1981 ttccatctgc atttgtttgc tgctggcttt aatcacgaag ccattatcat cggtttccca
     2041 actcaccccg gcagaaggta attctgctag ctggcatttt ccctgtaaac gtactctctt
     2101 ctccagcgtc tcggcatcac ggtaataatt ggcgtccagt acca
//
LOCUS       DAJTRH010000230         2046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTRH010000230 DAJTRH010000000
VERSION     DAJTRH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..913
                     /gene="ssuA"
                     /locus_tag="OO339_004108"
     CDS             <1..913
                     /gene="ssuA"
                     /locus_tag="OO339_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5928493.1"
                     /translation="TFAVAAESSPEALRIGYQKGSIGMVLAKSHQLLEKRYPQSKISW
                     VEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVGVEPPKPKAEVILVAEN
                     SPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQPTYLTPADARAAFQQGN
                     VDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPYAEKNGAFIQGVLATFS
                     EADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKPVNAEVAALQQQTADLF
                     YENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            910..>2046
                     /gene="ssuD"
                     /locus_tag="OO339_004109"
     CDS             910..>2046
                     /gene="ssuD"
                     /locus_tag="OO339_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5928494.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVA"
BASE COUNT          465 a          560 c          569 g          452 t
ORIGIN      
        1 tacttttgcg gttgctgcag aatcctcgcc tgaagcgtta cgtataggct atcagaaagg
       61 cagtattggt atggtgctgg caaaaagcca ccagttactg gaaaaacgct atccgcaatc
      121 aaaaatctcc tgggtggagt tccccgcggg tccgcaaatg ttggaagcgt taaacgttgg
      181 cagtattgat ctcggcagta ccggggatat tccgccaatc tttgcccagg ctgccggggc
      241 tgatttggta tatgtgggcg tcgagccacc gaagcccaaa gccgaagtga ttctggtggc
      301 agaaaacagc ccgatcaaaa ccgtagccga tcttaaaggt cacaaagttg cctttcagaa
      361 aggttccagt tcacacaacc ttttactgcg tgcactgcgt caggccggac ttaaattcac
      421 cgatatccag cccacttacc tgacgcctgc cgatgcccgc gccgcgttcc agcaaggtaa
      481 cgttgacgcc tgggctatct gggatcccta ctactccgct gcgttattac agggcggcgt
      541 gcgggtgctg aaagacggca ccgatctcaa tcaaactgga tcgttttatc tggcagctcg
      601 tccctatgca gaaaaaaacg gcgcttttat tcagggcgta ctggcaacct ttagtgaggc
      661 cgatgcgtta acccgcagcc agcgcgaaca aagcatcgct ttactggcaa aaacgatggg
      721 cttaccggca ccggtgattg cctcttactt agatcatcgc cctcctacca ccatcaaacc
      781 ggttaacgcc gaggttgccg ccttacagca gcaaacggca gatctgtttt atgaaaaccg
      841 tctggtgccg aaaaaagtcg atattcgcca gcgcatctgg cagcccactc aactggaagg
      901 aaaacaatta tgagtctgaa tatgttctgg tttttaccga cccacggtga cgggcactat
      961 ctgggaacgg aagaaggttc acgcccggtt gatcacggtt atctgcaaca aattgcgcaa
     1021 gcggcggatc gtctgggcta taccggtgtg ctgatcccaa cggggcgatc gtgtgaagat
     1081 gcgtggctgg ttgcagcgtc gatgatcccg gtgacgcagc ggctgaagtt tcttgtcgcc
     1141 ctgcgtccca gcgtaacctc acctactgtt gccgcccgcc aggccgccac gcttgaccgt
     1201 ctctcaaatg gtcgtgcgtt gtttaatctg gtcacaggca gcgatccaca agagctggca
     1261 ggcgacggcg tgttccttga tcatagcgag cgctacgaag cctcggcgga atttacccag
     1321 gtctggcggc gcttattgct gggggaaacc gtcaatttca acggtaaaca tattcatgtg
     1381 cgcggagcaa aactgctctt cccgccgatt caacagcctt atcctccgct ttactttggc
     1441 ggatcgtcag atgtcgccca ggagctggcg gcagaacaag ttgatctcta cctcacctgg
     1501 ggcgaaccgc cggaacttgt taaagagaaa atcgaacaag tgcgggcgaa agctgccgcg
     1561 tatgggcgca aaattcgttt cggtattcgt ctgcatgtga ttgttcgtga aactaacgac
     1621 gaagcgtggc aggccgccga gcggttaatc tcgcatcttg atgatgagac tatcgccaaa
     1681 gcacaagccg cattcgcccg gacggattcc gtaggacaac agcgaatggc ggcgctacat
     1741 aacggcaagc gcgacaatct ggagatcagc cccaatttat gggcgggcgt tggcttagtg
     1801 cgcggcggtg ccggaacggc gctggtgggc gatggtccta cggtcgccgc gcgaatcaac
     1861 gaatacgccg cgcttggcat cgacagcttt gtgctttcgg gctacccgca tctggaagaa
     1921 gcgtatcggg ttggcgagtt gctgttccca catctggatg tcgccattcc ggaaattccc
     1981 cagccgcagc cgctgaatcc gcaaggcgaa gcggtggcaa atgattttat cccccgtaaa
     2041 gtcgcg
//
LOCUS       DAJTRH010000231         2043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTRH010000231 DAJTRH010000000
VERSION     DAJTRH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..243)
                     /gene="ospE2"
                     /locus_tag="OO339_004110"
     CDS             complement(<1..243)
                     /gene="ospE2"
                     /locus_tag="OO339_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HCR5928495.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQ"
     gene            complement(425..826)
                     /locus_tag="OO339_004111"
                     /pseudo
     CDS             complement(425..826)
                     /locus_tag="OO339_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1255..2043
                     /gene="virF"
                     /locus_tag="OO339_004112"
     CDS             1255..2043
                     /gene="virF"
                     /locus_tag="OO339_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5928496.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          626 a          362 c          381 g          674 t
ORIGIN      
        1 ttgtttttca tcaattccgg cttcatttat ctttgtcaac cattgcattt ctgtagcagt
       61 gaatttcaac gttttcgtcc ggttggcagc aggtatttgt aattcgtata tagctgcttt
      121 tttattgaaa acagtatatc catctgtagt aacagtagaa gataataata caggatttga
      181 tacttcaagg atgatatttt cttgtttttg tggaaggcaa gggaatatag tttgtgtaag
      241 catattgctt ccttatgtta tattttagta aagaatttat tttttacatt atagtcggct
      301 aaatgaagtt tactgtaaaa aaacgatact tttttcgtaa gcgatgagat gtggagccag
      361 aaaaatggct gcgttacgtc attgaactgt ccttgaccca cgatattcag acagtcgttg
      421 tatcttaagt tgacattacc cgctgacgtc gatattccct tggtgagaga taacccaacg
      481 cactatgcgg atggttttca ttgtaatgct cgatcgccac tgcaaggtta tgcaatgccg
      541 ttcttggttt cggcatgaac gcgatgcagt cttccttcat cgttttcatg aaccgttctg
      601 ctattccatt actctgaggg ctactgactg ccgtcgtgca gggctccaga ttcaactctc
      661 tggcgaactg ccgtgtttca tgagccgtat aagccgaacc gttatcggcc actggattgg
      721 ccgttccggt cctttatcgc catatcgctc ctgacatgac atcctgcacc gttgctttgt
      781 cataatctcc cgtgcctgct gcccagtcta tggcttcacg atcgcaatat ggaacgtaac
      841 gctgtcatcg ggagaatcgt caatgcggca ttcggttaac gcttaccaat atcaacacca
      901 accggatttg tggagcctcc agtctgaagg ggctttatgc gttccgtata ggatattatg
      961 atgctggagt tttgcgaagc tccactcctt tccgaacatt tacacagata ttgcctaaga
     1021 aaagtgaaac cataatatgg ttatagtccc tttcagtgca aatacttagc ttgttgcaca
     1081 gagaaataga agctgcataa gctctttctt caaaaaatgt aaataaagtt aaatatagga
     1141 aaaattactt aatctatctt aataacggaa agattttgta tacaatcact gttacagaaa
     1201 tttcttagtt actctgtaaa cactaaatat agtttggtat attctgttga atttatgatg
     1261 gatatgggac ataaaaacaa aatagatata aaggttcgct tgcataacta tattatttta
     1321 tatgcaaaaa ggtgttcaat gacggttagc tcaggcaatg aaactttgac tatcgatgaa
     1381 gggcaaattg cttttataga gcgaaatata caaataaacg tctccataaa aaaatctgat
     1441 agcattaatc catttgagat tataagcctt gacagaaatt tattattaag cattattaga
     1501 ataatggaac caatttattc atttcaacac tcctattctg aggagaaaag ggggttaaac
     1561 aaaaaaatat tcctcctctc tgaggaggag gtttctatcg atttgttcaa atctataaaa
     1621 gagatgcctt tcggcaaaag aaagatctat agtttagctt gccttttatc agctgtttct
     1681 gatgaggaag ctttatatac ttcgatatcg atagcttctt ctcttagttt ttctgatcag
     1741 ataaggaaga ttgttgaaaa aaacatcgag aagagatggc gtctttctga tatttcaaat
     1801 aacttgaatt tatcagaaat agctgttaga aaacgattgg agagtgaaaa attaacattt
     1861 caacaaatcc ttcttgatat tcgcatgcat catgcagcaa agcttttatt gaatagtcaa
     1921 agctatatta atgatgtatc aagacttatc ggaatatcaa gcccatctta ttttataagg
     1981 aaatttaatg aatattatgg tataactcca aagaaatttt acttatatca taaaaaattt
     2041 taa
//
LOCUS       DAJTRH010000232         1964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTRH010000232 DAJTRH010000000
VERSION     DAJTRH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO339_004113"
     CDS             complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO339_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5928497.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          622 a          332 c          311 g          699 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga tatcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg
     1021 ttagctattt cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac
     1081 ttaattttat caaaggactt aatattaaga gtattatttg acaggttagc gttactttga
     1141 tgtctgaagt taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa
     1201 agaggatgag ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc
     1261 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat
     1321 ttgaaactgg tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt
     1381 aaaaagcttt tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag
     1441 gatgagtttg ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt
     1501 atttttccat caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt
     1561 attttcataa aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt
     1621 atctaactta atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat
     1681 tttcactctg aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa
     1741 cagtattatt tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat
     1801 atgatagata aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa
     1861 aattagtaac aataaatgaa agaatcatgg gcgctcaaca aaaagagggt aatgctgcca
     1921 acttactgat ttagtgtatg atggtgattt taaggtgctt gcgt
//
LOCUS       DAJTRH010000233         1951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTRH010000233 DAJTRH010000000
VERSION     DAJTRH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OO339_004114"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OO339_004114"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5928498.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>1951
                     /locus_tag="OO339_004115"
     CDS             1647..>1951
                     /locus_tag="OO339_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5928499.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          539 a          403 c          493 g          516 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa a
//
LOCUS       DAJTRH010000234         1916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTRH010000234 DAJTRH010000000
VERSION     DAJTRH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(56..1705)
                     /locus_tag="OO339_004116"
     CDS             complement(56..1705)
                     /locus_tag="OO339_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5928500.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          508 c          491 g          465 t
ORIGIN      
        1 cctgaagagg ataattttac agccagcagg aagttagcgg ctggctgtaa aaatattatg
       61 gttcgccgta caaaccaatt aaacggcgta ccacaccgtt agtccagcca aacccatcct
      121 gcaacggata ctcgccaccg ccgccttcgc ggggaacacc atcggcaata tggtatttct
      181 cgatcatttt gtgctgttcc agatagaact gattcaccgt tttcagccag ctgcgcgcga
      241 tttcatcacc cagaaggtca tcgccataca ttttaaatcc ctgaattgcc atccattgta
      301 acggtgccca gccgttgggt ttatcccact gttcaccggt ttcgtactcg cttgccaaaa
      361 tcccgccagg tgtcagtaac cggctgcgta cggcgtttgc cagacgatcg gcctgttcat
      421 ggttcgccat gccgacatag agcggcacaa tggcggcagc ggaaaacagc gccagttgtt
      481 cgcgtcgcca gtcgtaatcg cggtagatgc cgttttcatc atcccagagg taacggttta
      541 ccgcatcgcg acgggcactg gccttctggc ggaacagcgc ttctgtctct ttctcgcctt
      601 tcagcgccga gatgttggcg atggcgctct ccagtttaaa caggaaggca ttcagatcga
      661 tggggatgaa ctgggtggta cgaatgctcg ccagacgacc agtatcacgc agccaacggg
      721 aagagtaatc ccaaccggag gccgctcccg cgcgtaaatc gcggtacacc tcgttgggcg
      781 ggcgaccaga atgtttcgcg gtttcaacgt cctcaagcca ggattcgtca cgcggcgtgt
      841 cgcgatcgtc ccagtaacgg ttgagcagcg atccgtccgg catccgcaca acatggcgat
      901 aggcctgatt agggatcaac gattctgcac cgtccatcca gaaggcatat tccattttaa
      961 ggtggtcgag atagcggcgc gcaccgcgta caccatcttc ttcaaacaac tccaccatca
     1021 gcgcaaaaac cggtggttgc gagcggctca aataataggt gcggttgccg ttggggatgt
     1081 gaccgtagtt ttcgatcatc caggcgaagt tatcggccat gcatttcagc aaatcttccc
     1141 gaccactttc cgccagtccc agcatggtga aataggaatc ccaatagtag gtttcgctaa
     1201 aacggccgcc cgggacaata tatgactgcg gtagcgccag cagagaagac cacggaatgt
     1261 gatcctgtgg ttcgcgggtt agcaccggcc acagctggtc gatatgctct ttcagggaat
     1321 tttgcgggtc cgatacatac tcgctggagt agacctccgg cagccagaag tgattttcaa
     1381 caaacttgcg caagtcaaaa tcacgatgac ggcgcacttt acggtagcgg attaaaatat
     1441 ccagcgggtc cattttgggt gcgcagtcgg gaaaggtttt actgtcgggg aaaattttcg
     1501 ccgactgaac atgctcgaac agttcgagat agcgatcggc aggcgtcagc gcatcagagg
     1561 cgggcagccc ttcaatcatc tcgggttccg gttctgcctc gatcatctca tccagtttta
     1621 attcatacgg gtccagttca tagcagagat cgacctcgat catcagttcg tctggattag
     1681 ggttttgaat tttctgattg agcataagga tccaacctcc gaaattcgtc gctaattatc
     1741 gggcttttct ttaagggtag tcattcgctc cgcctggtgg gcgacaaagc gtgcggtgga
     1801 tcacgtttat taacttgtca aaaaatataa cgcactaata tcaatggaat ttataaaaat
     1861 acggatttgg tgttgttttt cattttcaga acattcaatc ctgctctcgc cgcaat
//
LOCUS       DAJTRH010000235         1913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTRH010000235 DAJTRH010000000
VERSION     DAJTRH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(854..1327)
                     /locus_tag="OO339_004117"
                     /pseudo
     CDS             complement(854..1327)
                     /locus_tag="OO339_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1255..1621)
                     /locus_tag="OO339_004118"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="OO339_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1913)
                     /locus_tag="OO339_004119"
                     /pseudo
     CDS             complement(1623..1913)
                     /locus_tag="OO339_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          425 a          536 c          459 g          493 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccaaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgt atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggc gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgcggacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatcatgatg atgtcgctgt tgctgacagt ggtaatacgt tga
//
LOCUS       DAJTRH010000236         1906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTRH010000236 DAJTRH010000000
VERSION     DAJTRH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(156..668)
                     /locus_tag="OO339_004120"
     CDS             complement(156..668)
                     /locus_tag="OO339_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5928501.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            863..1627
                     /locus_tag="OO339_004121"
     CDS             863..1627
                     /locus_tag="OO339_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928502.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1578..>1906
                     /locus_tag="OO339_004122"
     CDS             1578..>1906
                     /locus_tag="OO339_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR5928503.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWR"
BASE COUNT          558 a          414 c          473 g          461 t
ORIGIN      
        1 catcagcgac cagacagcat tgagaatgct tcaggagtac atcaggatcc aatgccttgg
       61 ggggtagcgg taattttact cattatcctt caaatcagat tctgttgtca gaggaatggg
      121 ggaagctgga ttcgcaagag ctttttatgg gcagtttagc taaaaaaagg caacacgaag
      181 ctaaaactac caacataaag tgctgaccag ctagcagctg aaatcagact cactgtatac
      241 actttcactg gatgaagttg caacattcct gccaccaaag gagcaatgta acgcagcacg
      301 gcgataaagc gcgaagtgaa aagtattgac acagagttat tttgcaactg aaggcgaacc
      361 cgttctattg tattggaacg gctcgatatt atacatgcaa gttgagggat atgcccaatt
      421 attataccaa gatggtaatt aacgatagta ccgcaccatg cgccactcat gaccgtgatt
      481 ccagcttccc atggtgaaag agtcgtcttg ctgactgtaa ttacagtggc catcataatt
      541 gatgctggtg gcaatatcgc cgaaatgagt aaagtggatt ttgtgaacgc tattataaaa
      601 agaagccccc ataggcgata tggatgaagt acaaaatagt tcatcaaagc atttatccac
      661 tccatcaatg tctttccttc tgtgaatttt tctttgctat ttaaccgtga tacgacttaa
      721 tttaggctta aacaccttac atttacaagg tactcctgtg gggggccctg ccacggagcg
      781 tcgcaggagt gagtgatgag gaagaattaa agcaacctat tgtgacaggc agcgataaca
      841 gtaaatcaca ggaagcataa tcatggcaaa accggactgg gaggccatcg aatcggcata
      901 ccgggccgga gtccttagcc tccgtgatat aggcgagaaa tacggcgtta cagaaggggc
      961 tatcaggaag agggccaaaa agcttgattg ggcacgcagt ggcggtacgc aggtttgcaa
     1021 aaatggtacg caaaaaagga aagtgcgtac cagcagaaag cctgccatta ctggccttac
     1081 acaaaaaagt acgcaactaa aaacagaatc tacaccggat acgaaaccga tacgcggaat
     1141 gcgtaccgat cccccgacta acccattcca acccggtaac cagcaggcat taaaacacgg
     1201 tggttatgcc cgtcgccttc tgctcaaaga tgaggtgata gaggacgcta aagcgttgac
     1261 gctcgaggac gaattatttc gccttcgtgc taacaacctt gtcgctgcag agaatattgg
     1321 tcggtggttg gtgtcgctgg aagatgctaa tggggaccag gaaaggaaga tgctgattga
     1381 aaatatcagc gccgccgaga aagcaatgat gcgcaataca gttcgtattg agtccatcgt
     1441 tggcacgctt gcgacggtag gaaaaatatt tgctgataca gcctaccgca aggccgccac
     1501 tgataaggtg tctctggagg ctgatcgtct tcgccgtgat gcaggtattg atgatggcaa
     1561 tggagagcgt gacctcaatg acttctactc tgacatccaa accgacgctg aatccggctt
     1621 tacgtagttt ctggactatg cgggcacgta acaaagtgct ttatggtggt cggtcatcgt
     1681 caaaatcatg ggatgccgct ggcattgcca tatttctgtc gaataaatac accctgcgtt
     1741 tttgttgtgc ccgtcagatc cagaataaaa tcgaagagtc ggtgtatacc ctgctcaaaa
     1801 ttcagataga caggtttggt ctgcggcacc gtttccgtat tctgaacaac aaaatcatta
     1861 accgggttac tggctcggaa tttgtttttt atggattatg gcgcaa
//
LOCUS       DAJTRH010000237         1898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTRH010000237 DAJTRH010000000
VERSION     DAJTRH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            114..1718
                     /locus_tag="OO339_004123"
     CDS             114..1718
                     /locus_tag="OO339_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5928504.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          519 a          428 c          475 g          476 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctctgataga ttacgtcgat ctttttaatg
      601 atattgatag gcaagataac caagcagact ctgtgtccct atctgttaat gctccagaag
      661 taaaaccaga agttacgcct agcattacca tagcgagcct ctttgaacaa tacgaagcgg
      721 agaactatca gaactggaag cctgcaacct taagggaaaa caaggcgtca cacgctgcgt
      781 taatcgaaat attcgaccat ctgggtctca atgcagacgc taacagggct gacatgctta
      841 gggtccgtga tgtgctccag cagttaccta ggaaccgtaa gcagcgcttc aaggatgtcc
      901 cacttgctga cctgttgagc cgtgaagata agacagattg tctggatgtc gttaccatca
      961 acaacaagta tcttattaag atggctgctg tctttaggtg ggctgtgcgc aatgacttga
     1021 ttaaaaagaa catgactgag ggtctggagc ttaaagtccc tcagcgtaaa gcctcagggg
     1081 cccgtaacgc cttctctacg gagcaggtgg ggcaactact ggtcgcagct aaggcatact
     1141 ctcagaagac ctctggtaag ccgtatcatt attatgttac cgccttggct gctatcactg
     1201 gggcgagact caatgaaata gcccaactac aggtcaaaga cgtcaggacc actgaggcgg
     1261 gaaccgttta tattcatatc aatgaagacg atagtagtct acccggtaag agcatcaaga
     1321 acgctcatag tgaccgctgt gtgcctctgg tcgatggggc ctatggtttt atattggctg
     1381 actttatggc gcttgtagag acccgtagag gcgctgacgg tgatgacgct atggtgttcg
     1441 atggtctgcg tctcatgaag aacggttacg gtgagcaggt gagtaaatgg tttaatcgga
     1501 cgttgttgcc taaagtcctc gtagaccgta gcggcttagc gtttcactcg tttagacata
     1561 cagttgcggc ccagttaaaa caacatgggg tcgagttggc gtatgcacag gcgatcatgg
     1621 ggcatagttc agggtctatt acgtacgacc gttatgctaa agaggtcgag gttgataggc
     1681 tggtcaatgt gatggctgat gtttataagg agacctagag gagaccatag tctaggatga
     1741 cacttactac agacctgtgt ggtcatgttt ttgactcata gttttctcta cttgtcaagg
     1801 gataattaat acgactcact atagggagaa gggacacaaa aagttttctc ttctaggatg
     1861 acacttacta cagacctgtg tggtcatgtt tttgactc
//
LOCUS       DAJTRH010000238         1799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTRH010000238 DAJTRH010000000
VERSION     DAJTRH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..729
                     /gene="btsS"
                     /locus_tag="OO339_004124"
     CDS             <1..729
                     /gene="btsS"
                     /locus_tag="OO339_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5928505.1"
                     /translation="QRVMGTIKLYEAKNRLFSSINRTLGEGIAQLLSAQILAGQYERQ
                     KAMLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQASQLVQYLSTFFRKNLKRPS
                     EFVTLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELSQQQLPAFTLQPIVENAIKHG
                     TSQLLDTGRVAISARREGQHLMLEIEDNAGLYQPVTNASGLGMNLVDKRLRERFGDDY
                     GISVACEPDSYTRITLRLPWRDEA"
     gene            726..1445
                     /gene="yehT"
                     /locus_tag="OO339_004125"
     CDS             726..1445
                     /gene="yehT"
                     /locus_tag="OO339_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5928506.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            1492..>1799
                     /locus_tag="OO339_004126"
     CDS             1492..>1799
                     /locus_tag="OO339_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5928507.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
BASE COUNT          475 a          410 c          478 g          436 t
ORIGIN      
        1 cagcgcgtga tgggcaccat caaattgtat gaagccaaaa accggttatt cagttcaata
       61 aaccgcacgc tgggcgaggg gattgcgcaa ctgctttcgg cgcagattct tgcagggcaa
      121 tatgagcggc aaaaagcgat gctcacccag tcagaaatca aactgcttca cgcccaggtg
      181 aatcctcatt ttttgtttaa tgcgcttaac accattaaag cggtgatccg ccgcgacagc
      241 gaacaggcca gccagctggt gcagtatctt tccacttttt tccgcaaaaa cttaaagcgg
      301 ccttcggagt ttgttactct cgccgacgaa attgaacatg tgaacgctta tctgcaaatt
      361 gaaaaggcgc gcttccagtc gcggttgcag gtcaacattg ctattccgca agaattatcc
      421 cagcagcaat tgcccgcgtt taccctgcaa ccgatagtgg aaaacgccat taaacatggg
      481 acatcacaac tgctggatac agggcgagtg gcaatcagcg cccgacgtga ggggcaacat
      541 ttgatgctgg agatcgaaga caatgccggt ttgtatcaac cggtaaccaa tgccagtggg
      601 ctggggatga atctggtgga taagcgttta cgtgaacggt ttggcgatga ctatggaata
      661 agcgtcgcct gtgagcctga tagttacacc cgaataacgt tacgactacc atggagggac
      721 gaggcatgat taaagtctta attgtcgatg atgaaccgtt agcacgggag aacctgcgta
      781 tatttttgca ggagcagagc gatattgaaa tcgttggaga gtgttcaaac gccgtggaag
      841 ggatcggcgc ggtgcataaa ctgcgcccgg atgtgctgtt tctcgatatc cagatgccgc
      901 gcatcagtgg tctggaaatg gtggggatgc ttgacccgga acatcgcccg tatattgttt
      961 ttctcactgc gtttgacgaa tacgctatta aagcctttga agaacatgcc tttgattatc
     1021 tgctgaagcc aattgatgaa gcgcgactgg agaaaacgct ggcgcgattg cgtcaggagc
     1081 gcagcaagca ggatgtttcg ctgttaccgg aaaatcaaca ggcgctgaaa tttatccctt
     1141 gtacggggca tagtcggatt tatttgctgc aaatgaaaga tgtggcattt gtcagcagtc
     1201 ggatgagcgg tgtctacgtt accagccacg aagggaaaga gggctttacc gaattgacat
     1261 tacgtacctt ggaaagtcgt acaccactac tgcgctgcca tcgtcagtat ctggttaacc
     1321 tcgcgcattt acaggagatt cgtctggaag ataacggcca ggccgagttg attttgcgta
     1381 atggcttaac cgtgccggtc agccgccgtt atctgaaaag cttaaaagag gcgattggcc
     1441 tgtaaaagac tgctaaaatg gctttttgcc tcatcaacac ctgaaggcct catgataagt
     1501 aacgatattc tgcgcagcgt gcgctacatt ttgaaagcca ataataatga cctggtgcgt
     1561 attctggcgc tggataatgt cgaagccacc gcggaacaga tcgccgtctg gctacgtaaa
     1621 gaagacgaag agggttttca gcgttgtccg gacattgttt tgtcgtcatt cctcaatggc
     1681 ctgatttatg aaaaacgcgg caaggatgag tctgctccgg cactggagcc ggaacgtcgc
     1741 attaataaca acatcgtgct gaaaaaatta cgcatcgcgt tttcgctgaa aaccgatga
//
LOCUS       DAJTRH010000239         1767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTRH010000239 DAJTRH010000000
VERSION     DAJTRH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..482
                     /locus_tag="OO339_004127"
     CDS             <1..482
                     /locus_tag="OO339_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928508.1"
                     /translation="LYVGVYGGSPADIAAAIWRKAPPGIDMNGNTTFTVADKEYDPPY
                     PEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVV
                     SAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            484..1089
                     /locus_tag="OO339_004128"
     CDS             484..1089
                     /locus_tag="OO339_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5928509.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1076..1624
                     /locus_tag="OO339_004129"
                     /pseudo
     CDS             1076..1624
                     /locus_tag="OO339_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          423 a          418 c          492 g          434 t
ORIGIN      
        1 cgctttatgt tggcgtgtac ggcggcagcc cggcagatat cgcggcggcc atctggcgta
       61 aggctccgcc gggtattgat atgaatggca acacaacgtt cactgttgca gataaggagt
      121 acgatccgcc gtatcctgaa tacgtgatca gctggcagac actcaaaccc gtcagtctgc
      181 atgtcagtgt gacgctgaaa aaaagtgact acctgccttc agatattacc cgacaggtac
      241 agcaatccgt gctggacgca tttaatggta cagatggtgg tctgcgggca agagttgcct
      301 ctgttgtctc tgccgggcgc tactatgccg gcatttacaa aaccgatccg gaacatattg
      361 atattctggg ccttactgtg agccgtgatg gttcgtcatg gacaacggct gtcactttcg
      421 ggatagatga gattccggtt ctggatgtgt cggatatcag tgtgaaactc caggaggcat
      481 aacgtgcaga atgtggctgc cactgtgctt gcgcagtatg ctgccagccc ccgactcaat
      541 gccctcatta acagctttaa cgcagcgctt tcccccgaca gttttatcag tgatttttat
      601 ggtcttatct ggaacatcga taccgcagaa aagtatggtc ttgatgtctg gggaaagatt
      661 gtgggtgtca gtcgctggct gacggtaaag gacgatttta attacctggg cttcagcgag
      721 tccaggatgg acaccccggt aatggatgat ccctgtccgt ttaatcaggc accgttttac
      781 aacggaaaat cggatacccg gactgttgac ctgtctgatg ctgtataccg gcggctgata
      841 ctgatgaaag ccatgtcgaa cattactgac tgttccgttc cggatattaa cctgatgctg
      901 agatttatgt tcggaaaaaa acgccgggct tatgttctga ataatggcgg gctgaggatg
      961 agttacgtct ttgagtccgc gctctcgttg gcagaactgg cgattatcca gtcgtcgggt
     1021 gcactgccat ccccgccggg tgtttatgtt tcagtagttt taaaggagtc ccgtaatgaa
     1081 ggccagtgat aaaccccgcc agctggcggt cccctttgcg agtaccggag ataaaaaccg
     1141 tatcccggac aaggcgacac agcagaccag agagagcggt aatgctgcgt atgattcagg
     1201 ttttcctccg gtgaccatga cagcggtctc agcgggaggt ataccgccac acggcaagga
     1261 ttttaacggt ctgatgtacg atattaccgc agcaatacgg ttcgcccagg ctggcggttt
     1321 gtacacgtat aatgccggtt ttgcgggggc cattggtgga tatgcaaaag gagccattct
     1381 cgccggagtc gcaacaacag cggtctggct gaataccacg gacgataacc tgaccgatcc
     1441 tgaaggctcc gacagtgcgg gctgggtaaa tcttcttgag gatccgaaaa ggatattcct
     1501 gcggcagaag aacaatctgt cagaccttca gaataaaggg acggcacggg ataatcttca
     1561 ggtttacagt aaagagcagt cagatcaacg ctatgttcat cgggaaggcg ataaaataac
     1621 cggagagctg aaaatccgtg gtgttaatgc gctgaggatt ttcaacgaag cttttggcct
     1681 gatttttcgt cgttcggaag agtgcctgca ccttattccc accagtgaag gtcaggggga
     1741 aaatggcgat attggtcccc tgcgccc
//
LOCUS       DAJTRH010000240         1754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTRH010000240 DAJTRH010000000
VERSION     DAJTRH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            230..499
                     /locus_tag="OO339_004130"
                     /pseudo
     CDS             230..499
                     /locus_tag="OO339_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            496..722
                     /locus_tag="OO339_004131"
                     /pseudo
     CDS             496..722
                     /locus_tag="OO339_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            751..1341
                     /gene="ospG"
                     /locus_tag="OO339_004132"
     CDS             751..1341
                     /gene="ospG"
                     /locus_tag="OO339_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5928510.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1680..1754
                     /locus_tag="OO339_004133"
                     /pseudo
     CDS             1680..1754
                     /locus_tag="OO339_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          515 a          368 c          407 g          464 t
ORIGIN      
        1 ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag tgataggaca
       61 ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta ctcctctgcc
      121 atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct ggtgacgata
      181 tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa tggcacaggt
      241 taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg ctgcaaaaag
      301 catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg ttgaacggca
      361 gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg aatgcggtga
      421 tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg aaatgagcag
      481 gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca ggacaggaag
      541 cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg gacgaacgga
      601 ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac ggtcgtgtgg
      661 aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa aatctcttgt
      721 gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat tattcaaaca
      781 ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc cattctcggt
      841 aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga ttcatctgct
      901 ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga aatggcttgg
      961 caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt tatacagtat
     1021 ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag tgacattgat
     1081 acagctgata tccctgataa tatagagagc ctttatctac agttgatatg taaattgaat
     1141 gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga taaagaaagt
     1201 gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc tgcaaccaaa
     1261 aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt ttattcgtta
     1321 ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg ctcttctatg
     1381 agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta tgcaccgcca
     1441 gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa ccacccgaaa
     1501 aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca acaaggcgaa
     1561 tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt aaaccgtctg
     1621 gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga cactatcgta
     1681 tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa catttaccac
     1741 gggttgtggc cgga
//
LOCUS       DAJTRH010000241         1690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTRH010000241 DAJTRH010000000
VERSION     DAJTRH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..165
                     /locus_tag="OO339_004134"
     CDS             <1..165
                     /locus_tag="OO339_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane;protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IIC forms the translocation channel for the sugar
                     uptake; involved in N-acetylgalactosamine transport;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5928511.1"
                     /translation="FLFVCYIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASK
                     NEEEDYSNGI"
     gene            155..946
                     /gene="agaD"
                     /locus_tag="OO339_004135"
     CDS             155..946
                     /gene="agaD"
                     /locus_tag="OO339_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5928512.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            947..>1690
                     /locus_tag="OO339_004136"
     CDS             947..>1690
                     /locus_tag="OO339_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5928513.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICL"
BASE COUNT          411 a          408 c          440 g          431 t
ORIGIN      
        1 ttcctgtttg tttgctacat ccgggtcagc aacctgttgc cggttgccgt actgggcgca
       61 ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca accgcagccc
      121 gttgccagta aaaatgaaga agaggactac agcaatggga tctgaaatca gtaaaaaaga
      181 tatcacccgt ctgggctttc gttcgtcgct gctgcaagcg agctttaact acgaaaggat
      241 gcaggcgggc ggttttacct gggcgatgtt gccgatcctg aaaaagattt ataaggacga
      301 caaaccgggc ttaagcgcgg cgatgaaaga taacctcgaa tttattaata cccacccgaa
      361 tctggtcgga ttcctgatgg ggttattaat ttcgatggaa gaaaaaggag aaaaccgcga
      421 caccattaaa ggcctcaaag tggcactgtt tggcccaatc gccgggattg gcgatgcgat
      481 tttctggttt actttgttgc cgattatggc gggaatttgc tcatcatttg ccagccaggg
      541 aaacctgctg gggccgattc tatttttcgc cgtttacctg cttatctttt tcctgcgcgt
      601 cggctggacc cacgtcggtt attcagtcgg cgtgaaggcg atcgataaag tgcgagagaa
      661 ctcgcagatg attgctcgct cggcaaccat cctcgggatc acggtaatcg gcgggctgat
      721 cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc atcgacagta cccacagcgt
      781 cgcgctgcaa caggatttct tcgataaagt cttcccgaac attttaccga tggcctacac
      841 cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt
      901 gacttttgtg ctctctattg tttgttccgc attcggcatt ttgtaaatgg aacgaggcac
      961 tgcttctggc ggtgcctctt tacttaagga atttcatcct gtgcaaaccc ttcagcaagt
     1021 tgaaaactat acggcgttaa gtgaacgagc cagcgaatat ttattggctg tgatccgtag
     1081 caaaccggat gccgtgattt gcctggcgac cggagccacg ccattactga cgtatcatta
     1141 tctggtagaa aaaatccacc agcagcaggt tgatatcagc cagctcacct tcgtgaagct
     1201 cgacgaatgg gtggatctgc cattaacgat gcctggcacc tgcgaaactt tcctacaaca
     1261 gcatatcgtg cagccgctgg ggctacgtga agaccagctc atcagctttc gctccgaaga
     1321 gataaatgag acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga
     1381 tttatgcgtt ctcggattgg ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaag
     1441 cctacaaccg gcctgccata tcagtcaact tgatgccaga acacagcaac atgagatgtt
     1501 aaaaaccacg ggtcgccccg tgactcgtgg gatcacctta ggcctgaagg atattctcaa
     1561 tgcccgcgag gttttgttat tggtgactgg cgaaggaaag caggatgcga cagaacgttt
     1621 tctcacggct aaagtctcca ccgctatccc ggcttcattt ttatggctgc acagtaactt
     1681 tatttgttta
//
LOCUS       DAJTRH010000242         1679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTRH010000242 DAJTRH010000000
VERSION     DAJTRH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(1..800)
                     /locus_tag="OO339_004137"
                     /pseudo
     CDS             complement(1..800)
                     /locus_tag="OO339_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(851..1426)
                     /locus_tag="OO339_004138"
                     /pseudo
     CDS             complement(851..1426)
                     /locus_tag="OO339_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1430..1679
                     /locus_tag="OO339_004139"
                     /pseudo
     CDS             1430..1679
                     /locus_tag="OO339_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          357 a          482 c          422 g          418 t
ORIGIN      
        1 ccccgctcac caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc
       61 gcccgcaggg cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac
      121 acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga
      181 cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg
      241 gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
      301 atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
      361 gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg
      421 ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa
      481 ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc
      541 gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
      601 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
      661 ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca
      721 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
      781 atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg
      841 gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc
      901 gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac
      961 cattactcca gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg
     1021 ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta
     1081 gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta
     1141 ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     1201 catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc
     1261 tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga
     1321 cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt
     1381 tataattacc actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac
     1441 gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga
     1501 cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac
     1561 tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg
     1621 gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggg
//
LOCUS       DAJTRH010000243         1597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTRH010000243 DAJTRH010000000
VERSION     DAJTRH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..1018
                     /locus_tag="OO339_004140"
                     /pseudo
     CDS             1..1018
                     /locus_tag="OO339_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
     gene            1115..1267
                     /gene="hokE"
                     /locus_tag="OO339_004141"
     CDS             1115..1267
                     /gene="hokE"
                     /locus_tag="OO339_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5928514.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
BASE COUNT          462 a          329 c          393 g          413 t
ORIGIN      
        1 gcaccaaaag aaacattgca atatcagttg atccagaaaa agcggcatca gcattggagc
       61 tgtcaaaaga ccgacgcatt gaggatcttg aaaggcaagt tcgatttctt gaaacgcggc
      121 ttatgtatct aaaaaagctg aaagccttag ctcatcccac gaaaaagtga aagtactcaa
      181 cgagctaagg cagttttatc ctcttgatga gcttctcagg gctgcggaga taccgcgcag
      241 tacgttttat taccatctaa aggctctcag caagcctgac aagtatgcgg acgttaaaaa
      301 gcgtattggt gagatttatc acgagaatag aggccgatac ggataccgta gggtaacgct
      361 gtctcttcat cgagaaggga aacagattaa ccataaagct gttcagcgcc tgatgggaac
      421 cctctcactt aaagcagcga ttaaggtcaa gcgataccgc tcttacagag gagaggtagg
      481 gcaaaccgcc cctaatgttc tccaaagaga tttcaaggct acgcggccaa acgagaagtg
      541 ggttaccgat gttactgaat ttgcagtcaa tgggcgcaag ctgtatttgt ctccagtaat
      601 agatctcttc aacaacgaag ttatttctta cagcctttcg gaaagaccag tgatgaacat
      661 ggttgagaat atgctcgatc aggcattcaa aaagcttaat cctcacgagc atcctgttct
      721 gcactctgac cagggatggc agtatcgtat gagaagatat caaaatatcc ttaaagaaca
      781 tggtattaaa caaagcatgt ccagaaaagg caattgtctg gataatgctg tggtggagtg
      841 tttctttgga accttaaagt cggagtgttt ttatcttgat gagttcagta atataagcga
      901 actgaaggat gctgttacgg aatatattga atactataac agcagaagaa ttagcctgaa
      961 attaaaaggt ctgactccaa ttgaatatcg gaatcagacc tatatgcctc gtgtataact
     1021 gtccaacttt ttggggtcag tacaccctgg ggctgccact ccaaaccaga caatttggat
     1081 ggtagtccct tcttcgcatg gaggtaatat aaacatgctg acgaaatatg cccttgtggc
     1141 agtcatagtg ctgtgtttga cggtactggg atttacgctt ctggccggag actcgctgtg
     1201 tgagtttacg gtgaaggaac gtaatattga gttcagggct gttctcgctt acgaaccgaa
     1261 gaagtagccg ttgtgcgggg agtaatcccc gcatatccgg ttgtcaggtc aggatggtaa
     1321 ggcacctgct ttacactttc gcccgcggtc agtgatggct gcgggcgatt tcgttttaag
     1381 agcaatgatg agttttgccg acagaagtcc gaaacaggac tcactgtata aataaacagc
     1441 tattttgcta aggaagggta agataacggc gggtgcctga agctttccgg tttcaggtta
     1501 actctgaggt ttggaaagat gaagccccaa gagatatttc tatcaatcct ggggctgcca
     1561 ctccgaacct caacaactcg gatggtagtc ccttctt
//
LOCUS       DAJTRH010000244         1587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTRH010000244 DAJTRH010000000
VERSION     DAJTRH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..626)
                     /locus_tag="OO339_004142"
     CDS             complement(<1..626)
                     /locus_tag="OO339_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5928515.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFIN"
     gene            complement(740..1312)
                     /locus_tag="OO339_004143"
     CDS             complement(740..1312)
                     /locus_tag="OO339_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR5928516.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          472 a          334 c          316 g          465 t
ORIGIN      
        1 tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa aggtaacgtg
       61 agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac gatgacatcc
      121 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
      181 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      241 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      301 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      361 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      421 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      481 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      541 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      601 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      661 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa
      721 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      781 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      841 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat
      901 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      961 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
     1021 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
     1081 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
     1141 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
     1201 tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt gcatgcccgg
     1261 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc ataaaaactc
     1321 cattgatata atatatcctt taccatgaat catcacttat attaataagt gtgtttttga
     1381 ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta attacgcaaa
     1441 aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca agtttgttat
     1501 cagacattaa tgataattca gaatatattt atatagaaag atagatgaat ttattgatgt
     1561 cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTRH010000245         1566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTRH010000245 DAJTRH010000000
VERSION     DAJTRH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..879)
                     /gene="ltrA"
                     /locus_tag="OO339_004144"
     CDS             complement(<1..879)
                     /gene="ltrA"
                     /locus_tag="OO339_004144"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5928517.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAM"
     gene            complement(1404..1566)
                     /locus_tag="OO339_004145"
                     /pseudo
     CDS             complement(1404..1566)
                     /locus_tag="OO339_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          338 a          449 c          354 g          425 t
ORIGIN      
        1 cattgctgca tttgacgcac ccggcatgca ttttcggtac cgttcgccgt tgaaattctt
       61 cagcgtacgc actttgaagt catatccgag aaaggtgaaa ctcgttgcga cgtttctcct
      121 tttaaacgtg tcaatgtagg cgatattcgt tttccctgca ttcaggacca gcccgacttc
      181 cgagaagcgc tccgataacc tgttctttag ccttgttgca tcactcattc gtgaacaatg
      241 tactacaata tcgtcagcgt acctctcaaa cggtaccccc cgatattctc tttccatcca
      301 caaatcaaat gcatagtgaa ggaacaagtt agcaagtagc ggactaatga ccccgccctg
      361 cggagtgcct cgtgttcggg ttattaattc tccattttca caactctgca ttggcgcttc
      421 catccagcgt cgacagtaca ggatgaccca tttaggcatc ccgtggtgtt ccagagcttt
      481 gagcaccaga tcatgtctca catggtcgaa aaacgcactg atgtccactt ccagtatcca
      541 gctataacgc cagcaccgga tggcacattg tttcagagcg tcatgagctg atttgcctgg
      601 tcggtaacca tatgaatcag catggaaaat cgggtcaagc ttttcttcca tgaaaagttt
      661 tatcgctccc tgagcaattc gatcggatac tgttgggatc cccagaatgc gttccttgcc
      721 attcgacttc gggatccgtt tttccagcac tggtggcgga aaccatgttc ccgaacatag
      781 ccggttccag atcttgtata gattgccatc acgttgctgg tcgaacattt tcaaagtctg
      841 cccgtcgcat ccggggggct cccttgttgc gacgcacatc cagatatgat gcccagacca
      901 gtgcttttgg tatttcaaat gattttcgtt gcatcagtta cgtcatcccc ttttgggttg
      961 tgtcttctgc aatgaatgtg atccccatcc cttcgctcca gacccattac agatctttca
     1021 tcactactac gaatgggtcc gcccctgttt cccgatacgt gccggaaaca ggttctcacg
     1081 ttccctgaga tggtctggcc cgggatcctg ctgccttcac accggatgcc gcgcagtcag
     1141 taagcaggtg tcttctgcgc ttatcagggg acggctgtac ggccccattt tggcatcatc
     1201 taagatttcg atgcttcatc ggacagttta ctttcgttca gcttcccgaa cccacgtgac
     1261 agtagatctc tgccttttcc cgagtcgctc accacctcat tccttagaat gaagcagcac
     1321 cgggtcgttt ggtctgtgct cctgtaaacc ctgaccggag ggcccacctc tacccacctc
     1381 agagcatgga taacttatgc tgcaatttcc gaaccggcgt tacggtcatc acgaacgtag
     1441 acccacagcc gggcagtcct tgttttaccg ctgcccggct cctggaccgg aacggggata
     1501 tcatcggcat ggactttacc gggcatcagc acatactggc gcaggatgtc atacagcggt
     1561 tccagc
//
LOCUS       DAJTRH010000246         1464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTRH010000246 DAJTRH010000000
VERSION     DAJTRH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            698..982
                     /locus_tag="OO339_004146"
     CDS             698..982
                     /locus_tag="OO339_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5928518.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            982..1257
                     /locus_tag="OO339_004147"
     CDS             982..1257
                     /locus_tag="OO339_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5928519.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          397 a          367 c          361 g          339 t
ORIGIN      
        1 tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg caaaaacagc
       61 agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa caagtcccat
      121 tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca gccctaacag
      181 aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca cataaacagg
      241 ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta gtccaccggc
      301 atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact ggtgtgagcg
      361 gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg cccgctaacg
      421 cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc gcgcacagag
      481 gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac tggtgatatt
      541 cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc tacatgtaac
      601 caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg tctgaaagta
      661 atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa acaataccgc
      721 acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata aagtcgacca
      781 gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc tttctgcatg
      841 gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg atgtgacatc
      901 cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca gtactgacca
      961 tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact ttctgatttg
     1021 gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag aacggtgcag
     1081 tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga acagttgttt
     1141 cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat ccgttacgaa
     1201 cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa caggtagatt
     1261 gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat tgttatcagg
     1321 ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt acgatgaaat
     1381 aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc aaataaccca
     1441 tcagaaacgt tattgccaga catt
//
LOCUS       DAJTRH010000247         1457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTRH010000247 DAJTRH010000000
VERSION     DAJTRH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO339_004148"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO339_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5928520.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO339_004149"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO339_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1457)
                     /locus_tag="OO339_004150"
                     /pseudo
     CDS             complement(1292..1457)
                     /locus_tag="OO339_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          450 a          224 c          317 g          466 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcct
//
LOCUS       DAJTRH010000248         1438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTRH010000248 DAJTRH010000000
VERSION     DAJTRH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..1029
                     /gene="arsB"
                     /locus_tag="OO339_004151"
     CDS             <1..1029
                     /gene="arsB"
                     /locus_tag="OO339_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5928521.1"
                     /translation="NGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGF
                     SKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFREYASVMVPVDIAAIVA
                     TLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFVLEPLGIP
                     VSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYL
                     SGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVY
                     ANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPVLFVTLAAL
                     ALRLSFTL"
     gene            1042..>1438
                     /gene="arsC"
                     /locus_tag="OO339_004152"
     CDS             1042..>1438
                     /gene="arsC"
                     /locus_tag="OO339_004152"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5928522.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKV"
BASE COUNT          286 a          375 c          394 g          383 t
ORIGIN      
        1 aatggacgcg gtcgcttgct gtttacctgg attgtcctgc tcggtgccgc cgttgcggcc
       61 ctgtttgcca atgatggcgc ggcgcttatt ttgacgccga ttgtcatcgc catgctgctg
      121 gctttaggat tcagtaaagg cacgacgctg gcgttcgtga tggcggccgg attcattgcc
      181 gataccgcca gcctgccgct tattgtctcc aacctggtga atatcgtttc cgctgatttc
      241 tttggcctcg gctttcgcga atacgcctcg gtgatggtgc cggtggatat cgccgcgatt
      301 gttgccacgc tggtgatgct acatctctat tttcgcaaag atattccgca gaactacgat
      361 atggcgctgc tgaaatctcc cgcagaagcg atcaaagatc ctgctacgtt caaaactggc
      421 tgggttgttt tactgcttct gctggtggga tttttcgtcc tggaaccgct cggcattccg
      481 gtgagcgcca ttgcagctgt gggcgcactg atattatttg tcgtcgctaa acgcggtcat
      541 gcgattaata cgggtaaagt cctgcgcggt gccccctggc agattgtcat cttctcgctc
      601 ggcatgtatc tggtggttta tggcctgcgc aatgccggat taacggaata tctttctggc
      661 gtactcaacg tgctggcgga taacggcctg tgggccgcga cgctcggcac cggattcctc
      721 accgccttcc tctcttctat tatgaacaat atgccgacgg tactggttgg cgcgttgtcc
      781 attgatggca gcacggcatc tggcgttatc aaagaagcga tggtttatgc caacgtgatt
      841 ggctgcgatt tggggccgaa aattaccccg attggtagcc tggcaacgct gctctggctg
      901 cacgtacttt cgcagaagaa tatgactatc agctggggat attacttccg tacagggatt
      961 atcatgaccc tgcctgtgct gtttgtgacg ctggctgcgc tggcgctacg tctctctttc
     1021 actttgtaat gagatactga tatgagcaac attaccattt atcacaaccc ggcctgcggc
     1081 acgtcgcgta atacgctgga gatgatccgc aacagcggca cagaaccgac cattatccat
     1141 tatctggaaa ctccgccaac gcgcgatgaa ctggtcaaac tcattgccga tatggggatt
     1201 tccgtacgcg cgctgctgcg taaaaacgtc gaaccgtatg aggagctggg ccttgcggaa
     1261 gataaattta ctgacgatcg gttaatcgac tttatgcttc agcacccgat tctgattaat
     1321 cgcccgattg tggtgacgcc gctgggaact cgcctgtgcc gcccttcaga agtggtgctg
     1381 gaaattctgc cagatgcgca aaaaggcgca ttctccaagg aagatggcga gaaagtgg
//
LOCUS       DAJTRH010000249         1406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTRH010000249 DAJTRH010000000
VERSION     DAJTRH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..169
                     /locus_tag="OO339_004153"
     CDS             <1..169
                     /locus_tag="OO339_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5928523.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            154..792
                     /locus_tag="OO339_004154"
     CDS             154..792
                     /locus_tag="OO339_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5928524.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..>1406
                     /locus_tag="OO339_004155"
     CDS             1020..>1406
                     /locus_tag="OO339_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928525.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          348 g          394 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttc tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg actctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tgcaggaaaa cgcctgaggg gctggttgcc tgtagcctgc
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata agatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 cgaaaatatt taaatacatt gaaatccctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagt
//
LOCUS       DAJTRH010000250         1394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTRH010000250 DAJTRH010000000
VERSION     DAJTRH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..312
                     /locus_tag="OO339_004156"
     CDS             <1..312
                     /locus_tag="OO339_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5928526.1"
                     /translation="RKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFS
                     RGEVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMIT
                     R"
     gene            324..>1394
                     /gene="proA"
                     /locus_tag="OO339_004157"
     CDS             324..>1394
                     /gene="proA"
                     /locus_tag="OO339_004157"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5928527.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVN"
BASE COUNT          342 a          353 c          405 g          294 t
ORIGIN      
        1 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
       61 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
      121 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
      181 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
      241 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
      301 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
      361 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
      421 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
      481 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
      541 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
      601 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
      661 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
      721 gcctgaaaac cggtaatgcg gtgatcctgc gtggtggtaa agaaacctgt cgcactaacg
      781 cggcaacggt ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg
      841 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
      901 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
      961 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1021 cagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     1081 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     1141 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcgctgacac
     1201 agttgcaggc aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt
     1261 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     1321 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     1381 agcgttttgt taac
//
LOCUS       DAJTRH010000251         1374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTRH010000251 DAJTRH010000000
VERSION     DAJTRH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="OO339_004158"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="OO339_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5928528.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="OO339_004159"
     CDS             696..914
                     /locus_tag="OO339_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5928529.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..>1374
                     /locus_tag="OO339_004160"
     CDS             922..>1374
                     /locus_tag="OO339_004160"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="catalyzes the formation of FMN from riboflavin and
                     the formation of FAD from FMN; in Bacillus the ribC gene
                     has both flavokinase and FAD synthetase activities;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FMN
                     adenylyltransferase"
                     /protein_id="HCR5928530.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDI"
BASE COUNT          327 a          295 c          348 g          404 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatc
//
LOCUS       DAJTRH010000252         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTRH010000252 DAJTRH010000000
VERSION     DAJTRH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            400..1149
                     /locus_tag="OO339_004161"
     CDS             400..1149
                     /locus_tag="OO339_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5928531.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          387 a          278 c          275 g          426 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcac
//
LOCUS       DAJTRH010000253         1336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTRH010000253 DAJTRH010000000
VERSION     DAJTRH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="OO339_004162"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="OO339_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5928532.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="OO339_004163"
     CDS             complement(590..1255)
                     /locus_tag="OO339_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5928533.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          349 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctc
//
LOCUS       DAJTRH010000254         1331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTRH010000254 DAJTRH010000000
VERSION     DAJTRH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..482)
                     /locus_tag="OO339_004164"
     CDS             complement(<1..482)
                     /locus_tag="OO339_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5928534.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(473..775)
                     /locus_tag="OO339_004165"
     CDS             complement(473..775)
                     /locus_tag="OO339_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5928535.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1047..1319
                     /locus_tag="OO339_004166"
     CDS             1047..1319
                     /locus_tag="OO339_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928536.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          392 a          333 c          283 g          323 t
ORIGIN      
        1 cagactttat aaaacaaggt attaggtgcc acaactgact gaacaaaacc aaaacgctca
       61 taaaatgctc tggcgtcatc atcaattgca tgtaccataa cagccttaat gccaacctga
      121 tcagccagat tgacaacccg gtgaatggca tcacttagta gccatttacc gaaaccatgc
      181 ccctgggtac aaacatcaac agccagacgc cctaacagaa caacagggac aggattgggc
      241 atgttatgcc gcaaactgcg accaagctcg gcatgagtga cggaaccagt agctaatgag
      301 tagtatccca caatacgtaa agtatcctcc aagcaaataa cgaacgtgcg agaagcattg
      361 agcatctggt ttttcatagc ccgaccacgt aaccagttac ttagcacctc atttccacag
      421 tcaaatggct gaagtatatg ctcatccgtc aacaatgctg gcgcggtgac atttatttcc
      481 attcaggctt taccgccatc agacgttcac gcccttctgc attctgcaca ggagcttcca
      541 gttgcgtaat aaacgaatca taacgttcgt tatccagcac aaataaacgt tgatccagga
      601 taatgttttt agcttcctgg tacgccagtt ccatgatgaa atcggtacgg ttcttattga
      661 ccaagctcgc agcataatca ataacggctc gttcctcatc agttgcccgg atgttaatct
      721 gatgttctcg cggtgtttta cggacgcttg cagctgtaga cataaaaacc tccattggta
      781 acaatttgct atgcaattaa tgttatcgca aatggtaaca gaacgctata cagaatcact
      841 tacgggaaaa gcgatcccct gataaaaaca atggcagaga cctgaacatt gtgacgatga
      901 tctgtgtcgg cgcacaaatc atgtctgatt acggccgttt aatgtgattt tgataatgaa
      961 atgcctgaac cacggcagcc tggcggctgc aaatcggggt gtggcagaat atgagctttc
     1021 agagtaacca tccagaatga gttcccatgt cacacaacct ggagcaccag aaagtccaca
     1081 ccagaatggt gaaagaggtt ctgaaagccg tcgccagagc caataatcac ccttataagt
     1141 ccgtatttgc agactttatc accggtcatc cctcctgcac ggtctgtttc tgggaaacct
     1201 tccacaaaat gtacccagat tcaccgtacg aatacgtcac gttctgccat acctgccgtc
     1261 gttttgattt atacgaaaca gaagcggaga tgaaggcgga tgacccgaag tggtggtgat
     1321 tttcagcccc g
//
LOCUS       DAJTRH010000255         1316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTRH010000255 DAJTRH010000000
VERSION     DAJTRH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            119..1282
                     /locus_tag="OO339_004167"
     CDS             119..1282
                     /locus_tag="OO339_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5928537.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
BASE COUNT          431 a          288 c          326 g          271 t
ORIGIN      
        1 atcccactaa gaataaatct ccttacctca caacacgaca agcctgcatt gcggctcttc
       61 agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag gaaagaaaat
      121 gtcactcttc cgcagaggtg aaatatggta cgccagtttc acattgccga acggtaaaag
      181 atttaaacag tctcttggaa caaaggacaa aaggcaggcg acagaactcc atgacaagct
      241 aaaggctgaa gcatggcggg tcagcaaact tggtgaaata cctgatataa cgttcgagga
      301 agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa tcactggacg atgacaaaag
      361 ccggatcgga ttctggcttc aacatttcgc aggaatgcaa ctaagagaca ttactgaatc
      421 aaaaatttat tcagcaatgc agaaaatgac gaaccggcgt catgaggaaa actggaaact
      481 cagggcagaa tcatgcagaa aaaaagggaa acctgttcca gaatacacgc caaaaccagc
      541 gtccgttgca acgaaggcta cgcatctttc atttataaag gccctactaa gagccgcaga
      601 gcgtgaatgg aaaatgctgg ataaggcacc aattattaaa gtgcctcaac caaagaataa
      661 acggatccgc tggctggagc cccatgaagc acaaaggctg attgatgaat gtccggagcc
      721 attaaagtct gttgttgaat ttgcactggc aacaggctta agacgctcga acatcatcaa
      781 ccttgaatgg caacaaatag atatgcagcg ccgggtggca tggataaacc cggaagagag
      841 taaatcaaac cgcgcaattg gcgttgcgct gaatgatact gcatgtcgcg tattgaaaaa
      901 acaaatcggg aatcatcacc gttgggtatt tgtgtacaag gaaagctgta ccaaaccaga
      961 cggaacgaaa gcgccaacgg tcaggaagat gcggtatgac gcaaacacag cctggaaagc
     1021 ggcgctgaga cgagcaggta ttgatgattt cagatttcac gacttgagac acacctgggc
     1081 aagttggctg gttcaagccg gagtcccgtt gtcagtgtta caggaaatgg gaggctggga
     1141 gtctatcgaa atggttcgtc gatatgctca ccttgcacct aatcacctta ccgaacacgc
     1201 acggcaaata gactcgatcc tgaacccatc ggtcccaaat tcgtcccagt caaaaaataa
     1261 ggaaggtact aatgatgtgt aacttattga tttaaatggt gccgataata ggagtc
//
LOCUS       DAJTRH010000256         1299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTRH010000256 DAJTRH010000000
VERSION     DAJTRH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            82..1275
                     /locus_tag="OO339_004168"
     CDS             82..1275
                     /locus_tag="OO339_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5928538.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          387 a          273 c          298 g          341 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctca
//
LOCUS       DAJTRH010000257         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTRH010000257 DAJTRH010000000
VERSION     DAJTRH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO339_004169"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO339_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5928539.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          228 c          286 g          405 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 ccccttacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAJTRH010000258         1200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTRH010000258 DAJTRH010000000
VERSION     DAJTRH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            153..>1200
                     /locus_tag="OO339_004170"
     CDS             153..>1200
                     /locus_tag="OO339_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5928540.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVI"
BASE COUNT          337 a          298 c          287 g          278 t
ORIGIN      
        1 aagccgaatt tctggcaaat ctgattgcaa tgaaattaaa aggtgaaatc tgaaaaaaag
       61 aaagcctgca cactgtgcag gcctgaatga agaacctggg acatttattc atcactcgca
      121 gtaattttaa tctgagttga ggttaaaaaa caatgaacac cgataaacaa gtttacccac
      181 tgtattacga agcaaaaaat gacaaagtaa gaaaacgtct cggtattaaa ggcggttttt
      241 actgggctga agcgaaaaaa ttatccattg ccatctcccg tggtgctgtt gcgattgacg
      301 atgcaggcta cgatgaagat gactttaaaa aacctgttcg cgtcaatttg cccgttgttg
      361 atgacctccc gccagaaggc gtatttgata cggaattctg caaccgttac gaaaaaggcg
      421 gggaagatgg catcacaatg gtatttatcg catcttctcc ctctgttcag gacaaaccgg
      481 ccagcactga caataccaac gtcaacggcg aagacatgac agaaattgag gagaacatgc
      541 tcctgccagt ttccggtcag gaactgccca ttcgctggct tgcgcaacac ggcagcgaaa
      601 aaccagtaac gcacgtttca cgcgacgaac tccaggcatt acacattgct cgggatgaag
      661 aactaccagc tgttactgcc ctggctgttt cccacaaaac cagcctgctc gatccgctgg
      721 aaattcgcga tctccacaaa ctggtgcgtg atactgacag agttttccct aatccaggca
      781 attcaagcct ggggctgatg actgcttttt tcgaagcata catggacgca gactacaccg
      841 atcgcggtct gctgacaaaa gagtggatga aaggaaatcg tgtttcacgc atcacgcgca
      901 cggcttccgg cgctaatgct ggcggcggga acctcaccga tcgcggcgaa ggtttcgttc
      961 acgatctgac gtcactggcg cgcgacgtag ccactggcgt actggctcgt tcaatggacg
     1021 tggacattta taaccttcat ccggcacacg ctaaacgtgt cgaggaaatt atcgctgaaa
     1081 ataaaccgcc cttttctgtt ttccgcgaca aattcatcac catgcctggc gggatggatt
     1141 attcccgcgc catcgtggtt gcgtcagtga aagaagcacc gattggtatc gaggtcatcc
//
LOCUS       DAJTRH010000259         1199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTRH010000259 DAJTRH010000000
VERSION     DAJTRH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(1..411)
                     /locus_tag="OO339_004171"
                     /pseudo
     CDS             complement(1..411)
                     /locus_tag="OO339_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            334..585
                     /locus_tag="OO339_004172"
     CDS             334..585
                     /locus_tag="OO339_004172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928541.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            complement(605..>1199)
                     /locus_tag="OO339_004173"
     CDS             complement(605..>1199)
                     /locus_tag="OO339_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5928542.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          277 a          301 c          297 g          324 t
ORIGIN      
        1 tgtaagccaa cacaccgcca gttttagtgt tgtacgcata ccagggccag tgttgttgcg
       61 ccttgctgcc aacgaagcgc cactgctcat caagttcaca gataagggca acatcagcgt
      121 gagcgactgg cgcagatgtt atccgccggg gcgagagctt tttaatgtcc gtatgacggt
      181 attgatgccg attttcagtg tcctggcggt atcacggccc cctgcgccgt tatgtgccat
      241 ttcaacgatc tgctccttaa cgccgagttt tctggcttca taagagtaag tgagctggaa
      301 aacacgttta catgagcgac aacggaaacg ctcatgccgg gaacaactac ggccatgtcg
      361 ataaacttca tcggaattac agcgcggaca atgaacagta acggtagtca tggaaaacct
      421 caaataccag tagattacca gaaacttcta tcgattgaag acattacctt ttgccgcaat
      481 aggtggggta acattggtga aaacgcatta cgccgggtgg cagttggaaa aaaattatct
      541 tttttcggct ctgatcgtgg tggagaaaat gcggcaataa tctaaatagc tgcgcttaat
      601 accgctacac ttttgccagc ccatgtttgc ctccggggaa tgggctgacg gtttccataa
      661 aatggcgaac ttttttcaac agttgccaca ttgagcggca ctgatgatta cgcgttattg
      721 tgtcgtgaag tgcctgccat agccgttcaa catgattcac ccatggcgag taaaccggct
      781 gataaatgcc cctgaacttc gggttctcct tcagccagct ctgtgtttcc cggcttttgt
      841 ggataatgta gttgtccacg atcagcgtga tggttttcgc acgacggtat gtcgctttaa
      901 gccgcttcag caggctgatg aacagcgccg aacttttgct gttgccgccc acatagctga
      961 ctttacctgt cccgctgtgc agcgctccgg ccagataata tttttcattc tgtcccggcg
     1021 tgaccacccg tttttgctgt ccgcgcagtt gccagtccgc accgattttg ggatgaagat
     1081 ggatatccac ttcatcttca taaaagaccg gatgctctgc gctgcattcg tccagtgctt
     1141 tatggattgc tgccatcttt tcatctttat gcgggtcacg gatacgcaga gttggcgca
//
LOCUS       DAJTRH010000260         1196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTRH010000260 DAJTRH010000000
VERSION     DAJTRH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT          344 a          283 c          247 g          322 t
ORIGIN      
        1 gcattacgca taaaaaaacc gcttcagcgc ggctatgcgc tcgagtaacg ttcgggtttc
       61 cacgcccggc ccccgtttta tgaggtgcag atgcactata attccacccg ttctggtttt
      121 caatagctac attcaacatt ttctcttacc tttcatcacc gaagtgaact ttgttgatgc
      181 ggtgcctggt gcctccaggt gacgttaacc agttaacaat tacagttggc tttcccaccc
      241 aaaccaataa ggactaacat gacttttaac tgtgccgcgt gcgcatagcc gcattcaccg
      301 catcacaaaa ttcactttaa aaagggcgga catcagccag caatgaaact gatgccgcca
      361 aaggtaccaa tcaacatgga gtgttgtggc gtggttgtca cttaagcgta tggtcaacct
      421 gacaacccgg tgtcctcaac ggggaaggaa taactccgcc atacttaccg ccgcgtcatt
      481 tcgcgttgtg tgcctgcttt taactacgtc aggcgaggtg gttcctgtta ttccccaaca
      541 acaagaaatt tgtataatct gaatacccca acaataagaa gagtgagcaa gtgatgtatt
      601 agcgatgtca gctctcaaag agactgcatc aatgttaaaa ttattcaatg aagcaaaaac
      661 tcaggctgag gttgatcgcg ctgtgtctga aataaactct aagttgtcaa gcattcagct
      721 tgaatatttg tcatcatcat tgatgagctg acaatctcta aaagcaatga ggctgcgctc
      781 aaagcaaaac tcacggagat agaaaagttt aagttccaaa gtgaagactg cgaacctcgc
      841 taattgatta ctggaacgat tgtgtatgcc agacagtgca atactagctc tggcaagatt
      901 accatgtatt tttgtccaga atgttttgcg aagagagcaa tatctatatt gcaacctttc
      961 cctatcgagg aaggcgataa atatacgaaa actcaatgcc cctcctgtaa aaatgagtat
     1021 ctcatggact acaatttcaa taatgatttt tgagtcacgg tattcagatt tttaaagaac
     1081 atgccggata ctcacccgtg tccggcgcac gcactccacc tcacccgtgg agaactcctt
     1141 aattaccaac cttggcttcg ttggttagcc attaacgctg gtatgtaatc attctg
//
LOCUS       DAJTRH010000261         1159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTRH010000261 DAJTRH010000000
VERSION     DAJTRH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(57..407)
                     /gene="tnpB"
                     /locus_tag="OO339_004174"
     CDS             complement(57..407)
                     /gene="tnpB"
                     /locus_tag="OO339_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5928543.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            complement(404..1078)
                     /locus_tag="OO339_004175"
     CDS             complement(404..1078)
                     /locus_tag="OO339_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5928544.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRLAKQALQPGAVVARIAREHDINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIRE
                     MKGGIR"
BASE COUNT          258 a          296 c          300 g          305 t
ORIGIN      
        1 ctgttgctta cgcaacatgg ctttaagcag ctcaatatca tcggggagtt cattgttcat
       61 tcccttgttt tatcacgggt tatatccgga tgccaggccg ttctgtccgt ttgggatgtt
      121 gccacgcgat cccctccagt agcatggata actgagctgg cgtcaggtgc actttccctt
      181 cccgggtcac cggccagacg tttggtgaac aggcataacc cgtcacgatc ggcccacagt
      241 attttcacca ttttgccact gcggccccgg aagacgaaga tatgcccgga gaacgggtca
      301 tctttcagcg tgttctgcac cttcgaagcc aggccattga agccacaacg catatctgtg
      361 atgccagcga tgatccagat tttggtaccg gtcggcagcg ttatcatcgg ataccccctt
      421 tcatttcgcg gattagcgcc cgtaacagtt ccggagtgag agggtcaaac agttttacca
      481 cacctgattt aagatgcagc tcgcaccgtg ggacgtttcc gggagccccc tcagggcgct
      541 catcatgctt gttacgccag aagggatttg taactggtct ggtcggctcc ggcgtatcag
      601 tcagtgccac cgggacaggc atgcattcct gtatgtcatc atcgctcagt aagccgtcct
      661 cgtactggct tttccattta aacagcaggt tatcattgat atcgtgttct ctggcgatcc
      721 gggcaacaac agccccaggc tgtaatgcct gcttagccag acggacctta aattcacggc
      781 tatagctggt tcgccgttct tttcgccatg agccttctct gatttgaggc tctgttaatt
      841 ccttctttct gttggcataa aggatggcgt caagttgagc gaatgaaact gaatcgggca
      901 atggccatgc gataccggat gcaagaaatc gctgaaaaag cgtatgtatt gtggaatgac
      961 tgagacccag acgctgagcg atggcccgga tggtcagttt atcttcaaat cttaaacgca
     1021 gggcatcagg caaataagaa cggaagcagg gaatatcttt tgttgtctgg gaattcatcg
     1081 ttcgtgtcca tcaatataga tgggcgcgat tgttgccaga caggacaatt ttcacaagac
     1141 gtcgctgatg gggcgctta
//
LOCUS       DAJTRH010000262         1146 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTRH010000262 DAJTRH010000000
VERSION     DAJTRH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..167
                     /locus_tag="OO339_004176"
     CDS             <1..167
                     /locus_tag="OO339_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR5928545.1"
                     /translation="LFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHN
                     VFRITLPQRN"
     gene            complement(275..1126)
                     /gene="hchA"
                     /locus_tag="OO339_004177"
     CDS             complement(275..1126)
                     /gene="hchA"
                     /locus_tag="OO339_004177"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5928546.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
BASE COUNT          295 a          265 c          278 g          308 t
ORIGIN      
        1 aactcttccg tagattttgg cggggagata attcgcgtca ttccgtaggt cagggacttg
       61 gcctttcttt agtcaaagcg attgccgaat tacatggagg aagtgctacg tatcactatc
      121 tcagtaagca taacgtgttc cgaattacgt taccgcaaag aaattaacat cgggcttcag
      181 tacgccataa gttaaccgtg aaaaaacgca gccacgtcgt gagtattaac gtggctgcga
      241 ttgattatgc gcttacatca caatgtaaca gggattaact cgcgtaagct gccagcattt
      301 cctgcgccgc cagtttaccc aacgcattcg ctgcaaaagg actgtcgccg gtgagacgtt
      361 tacggtcctt atgtactcgc ccggtgatgt cgtcattaat gatgttcatg cccattttct
      421 tcaactcttc gccgaagtac caggtgagat gacccggcat atagccaata tctggcgttt
      481 gtttgtctgc ggcgtctggg aatgcgcaaa tagaataacc attcagtggg ttatcgctgt
      541 ggcgaagtgc cagaaaagcc gccggaccgt ggcaaaggga gataacaaaa cggtcatttt
      601 tgattgccca ctgtaaagcg gcagccacgt cctggctttc tggtaaacca ataagtgcgc
      661 catgcccacc aggaacaaag attgctgcat attcgctatc agcgttgagg ctggcgacaa
      721 catccgcgag tttcttcgga ttacggaata acgatttatg ctgttcaaag aatggcatta
      781 ctttttcatc tttgtgcggc atagcccagt attcaaactt ggtcatcaga ccggaaatgg
      841 tcgccacttc gaattcgaaa cctgcagcat ggagatgata caacggcagc aacgtttcaa
      901 tcggatggtt accggtcgag aacagttttc cgttatcggt cggcaaataa cgttcgtccg
      961 cggcgatcac cagaatttta tgtttaccgc gatacggttt tggatagtcc acgccatcaa
     1021 gatcagagac gggactcgta tattggctaa gcgaatattc tgaagggaag aacgcattat
     1081 cttcagcaat atcgacctgc gggtttttac ttgtttgaac agtcatagtg tattccttat
     1141 tgttgc
//
LOCUS       DAJTRH010000263         1138 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTRH010000263 DAJTRH010000000
VERSION     DAJTRH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.271"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..229)
                     /locus_tag="OO339_004178"
     CDS             complement(<1..229)
                     /locus_tag="OO339_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HCR5928547.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCA"
     gene            753..1138
                     /locus_tag="OO339_004179"
                     /pseudo
     CDS             753..1138
                     /locus_tag="OO339_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          362 a          259 c          201 g          316 t
ORIGIN      
        1 gcgcacagga gtgacgaatg atctccagac cttcttcgtc tttggcggta atgatcgaca
       61 gttgtgcgtc gttttcaatc agatcgcaag catcaaccag ttcgccatta acgcgccctg
      121 cgatacaggc tttcgccaga cctggaccaa tgtccagcgc aacatccatg gggcttacag
      181 cgtggtcgta atggcgttgg ctgccatcag gaagagttat aacaggcatt ttatatcctt
      241 atttgcagtg gtgacccaca cgaaagatca catacaaaga aaaatttgtt tattaacagt
      301 tcattgcgaa accatctagc caacaaatgc taaattggtt cgcaatcagg tacacaaatg
      361 aaagacatta acctctcacc ttccacccgg ttgaacgatg ttaacactaa aaaaagggag
      421 attgtacctt tccgtttcac attgattcga ttcgcaattc gtttgtagtg agtacaccag
      481 caaaacgctt aaagaaccac tgataacatc acggtgcgtc tgaaaggttt actgcgagcg
      541 gatttacagc agcataaact gaatccagca aagttcgttg cgtgatttgg cggtagtatt
      601 ttaaacgctc atgagattaa tgacgaagtg gtcatatcac aatgataaaa gtgacacaat
      661 tcttataaca atttttcgtg cacatttcgt tctggcgata ataattaatc atcatcctca
      721 aaacctccag atatatataa ggcgaataga ttatgagtca aaacgatatc attatcagaa
      781 ctcattataa gtctcctcat agattgcaca tcgatagcga cataccaaca ccttcatcag
      841 agcctattaa taaatttgcg cgccagctca tcaccctact tgatacctct gacttaagtt
      901 cgatgctgtc atactgtgtt actcaggaat ttaccgcaaa ctgtcgaaaa tatcacaaaa
      961 ttgttattcc actgcccttt ttaccattaa ctttgccact tcacccatcc atgcagaaaa
     1021 tatactcatt acattacact ataaaaaaga aatcatttcc ttattactag aaaccacgcc
     1081 tattaaagct aaccatttgc gaagcatact ggattatatt gaacaggaac agggtaat
//
LOCUS       DAJTRH010000264         1128 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTRH010000264 DAJTRH010000000
VERSION     DAJTRH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            37..987
                     /gene="virK"
                     /locus_tag="OO339_004180"
     CDS             37..987
                     /gene="virK"
                     /locus_tag="OO339_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5928548.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSGYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
BASE COUNT          331 a          210 c          232 g          355 t
ORIGIN      
        1 taaaaatact cacattagcg aagcaaaagt aacaatatgt tttctataag taacttatca
       61 tttatcggtt tccttaaaag gattattttt tcctcagatt cactaccggg gaagtgggaa
      121 cacagaaaat ttcggttcat gtacattttg cgatgttcta taaatccggt tgtcagtatt
      181 cgatattatt atgaactgcg ttccttgccg tgcattgagg atattctggc aatacacccc
      241 acgttgccag caagaattca tcgcccttat ttgcataaag gggggcgcgc ctggacccgt
      301 ggacaatata ttcttgagca ttatcgcttt gtgcaaaatt tgccagaaaa atattctaaa
      361 tttttgtttc cacaaaaatc ggtatcatta gtccaattca tcgggaaaga tggagagaat
      421 tttgatatcc agtgttctcc cagtggtttt gaccgggaag gagagttgat gttgtcattg
      481 ttttacaata aaacagttat tgcccggtta acattttcag tcattctgac tcaaaatgga
      541 cacattgctt ttattggcgg attacaggga gcccctaaaa ataccggacc tgatgttatt
      601 cggtgcgcaa cccgggcctg ttatgggatt ttcccaaaac ggattatttt tgaagcattt
      661 tgtgcgttga tgaaagcttg caatatctct gagtgtctgg cagtcagtga gcacagtcat
      721 gttttccggc aattgagata ctggtatcag aaacgcaaaa cctttgttgc ggtctatagt
      781 gatttctggg agtctgtagc aggaaaaacc tgtggtgact ggtacagatt accaacccag
      841 gtgattcgaa agccgctaag cgatatagcc agtaaaaaac gctctgggta ccgaaaacga
      901 tacgctttgc tggattatat ccatgaaact actatccgct ctttggatgc atatcctgta
      961 cactcagaac atcagaattt aaattaaggt ttgatgtatt ctctgaataa aacagtaatg
     1021 ataattttgg tagaggcatt cgcactaaat aatgaaaaaa tataaatctg agtttattcc
     1081 tgaatttaag aaaaattacc tttcccctgt ttactggtct acatggtt
//
LOCUS       DAJTRH010000265         1112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTRH010000265 DAJTRH010000000
VERSION     DAJTRH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.273"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            80..259
                     /locus_tag="OO339_004181"
     CDS             80..259
                     /locus_tag="OO339_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928549.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="OO339_004182"
     CDS             311..505
                     /locus_tag="OO339_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928550.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          335 a          254 c          260 g          263 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gt
//
LOCUS       DAJTRH010000266         1093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTRH010000266 DAJTRH010000000
VERSION     DAJTRH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(13..>1093)
                     /gene="rspA"
                     /locus_tag="OO339_004183"
     CDS             complement(13..>1093)
                     /gene="rspA"
                     /locus_tag="OO339_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5928551.1"
                     /translation="YLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAV
                     DMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIR
                     VQCGIPGMKTTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGF
                     NEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVG
                     EVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSP
                     VCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEK
                     LAAKYPYEPAYLPVARLEDGTLWNR"
BASE COUNT          272 a          287 c          267 g          267 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taa
//
LOCUS       DAJTRH010000267         1062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTRH010000267 DAJTRH010000000
VERSION     DAJTRH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>1062
                     /locus_tag="OO339_004184"
     CDS             <1..>1062
                     /locus_tag="OO339_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5928552.1"
                     /translation="VDAERGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGL
                     MDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENR
                     VWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQR
                     LQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQH
                     GFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSK
                     RLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLA
                     AYIFPGRNLSLTVDADPQAE"
BASE COUNT          306 a          252 c          262 g          242 t
ORIGIN      
        1 gtagacgctg aacgtggcgt aattactgag gaatggcgtg cccatcagga tgcgaaatgg
       61 cgcacctctc aggcgcgccg ccctttcctg ctggctaata cccgtaattt agaccgtgaa
      121 cctatcggcc tgatggatac tgtcgccacg gtcacgccgg cacaattgcg ccaattttat
      181 caacgctggt atcaaccaaa taatatgacc tttatcgtgg tcggcgatat cgacagtaaa
      241 gaagcgctgg cgctgataaa ggataattta agtaagcttc cggctaacaa agcagctgaa
      301 aatcgcgtct ggccgacaaa agccgaaaac cacctgcgct ttaatatcat taatgataaa
      361 gaaaaccggg tgaacggcat cgcactctat tatcgcctgc caatggtaca agtgaacgat
      421 gagcaaagct ttgtcgaaca agctgaatgg agcatgttag ttcagctgtt caatcaacgt
      481 ctgcaggaac gcatacagtc gggcgagttg aagactattt ctggcggcac tgcgcgcagc
      541 gttaaaattg cacccgatta tcagtcgctg tttttccgtg taaatgcacg agacgataat
      601 atgcaggatg ctgcgaatgc attaatggca gagttggcaa ccattgatca gcatggtttt
      661 tctgctgaag aactcgatga tgtcaaatct acccgcctca cctggctgaa aaatgcggtt
      721 gatcagcaag ctgaacgtga tttacgtatg ctgaccagtc gcctggcatc cagctcatta
      781 aataatacgc cgttcttgtc gccggaagag acatatcaac tttcgaaacg tctgtggcag
      841 caaattaccg tgcaaagtct ggcggaaaaa tggcagcagt taagaaagaa ccaggacgca
      901 ttttgggagc aaatggtaaa caatgagctt gccgccaaaa aagcattgtc tcctgcggct
      961 atcctggcgc tggaaaaaga gtacgccaac aaaaagctgg cggcttacat cttcccaggc
     1021 agaaatttat cgttaacagt agacgctgac ccacaggcgg aa
//
LOCUS       DAJTRH010000268         1013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTRH010000268 DAJTRH010000000
VERSION     DAJTRH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>1013
                     /gene="sat"
                     /locus_tag="OO339_004185"
     CDS             <1..>1013
                     /gene="sat"
                     /locus_tag="OO339_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR5928553.1"
                     /translation="RSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTS
                     FDQPDWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKN
                     ITNNGFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISID
                     KGAKVIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIA
                     RNMASVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKA
                     TVSMNNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLA
                     QAD"
BASE COUNT          341 a          202 c          202 g          268 t
ORIGIN      
        1 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
       61 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
      121 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
      181 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
      241 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
      301 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
      361 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
      421 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
      481 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
      541 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
      601 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
      661 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
      721 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
      781 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
      841 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
      901 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
      961 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca gat
//
LOCUS       DAJTRH010000269         1004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTRH010000269 DAJTRH010000000
VERSION     DAJTRH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..534
                     /locus_tag="OO339_004186"
                     /pseudo
     CDS             1..534
                     /locus_tag="OO339_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          309 a          220 c          210 g          265 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatacaaa aacaaataat
      721 accgttgcaa taattccttt tagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 aaaatttgta caacatatta aaatatgttc atttcgttat aatacacaca aggaaatatt
      841 aagttagata atatattcta aataaccata gataaaaacg cacataatgg ggaatatttt
      901 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tcttgttgat
      961 ggaaaaacga atccgaataa agcaacaaaa gcattacaaa aaat
//
LOCUS       DAJTRH010000270         1001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTRH010000270 DAJTRH010000000
VERSION     DAJTRH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..93)
                     /locus_tag="OO339_004187"
     CDS             complement(<1..93)
                     /locus_tag="OO339_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR5928554.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISW"
     gene            complement(159..398)
                     /locus_tag="OO339_004188"
     CDS             complement(159..398)
                     /locus_tag="OO339_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5928555.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(533..>1001)
                     /locus_tag="OO339_004189"
     CDS             complement(533..>1001)
                     /locus_tag="OO339_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5928556.1"
                     /translation="GESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSS
                     LIKTIPRKGVRYLGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEF
                     STEELLPIKHTDKNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          288 a          231 c          167 g          315 t
ORIGIN      
        1 ccatgaaata aaaataagaa agagcgcaac aaataaaaga tcgactctta gtaaccatat
       61 tgctgccatt gaaagactcc atgtaccata caaggttgct gccagacata ttcctttacg
      121 tctgacagcc tctagagagt ttttttattt tttataagtt acggattact cgttccggaa
      181 atcgttacct gttgaattgc tgcagcgata gcgtcgaatg catctttaat tgcgcccagg
      241 aagcctgagt tctgatcgcc aaagatgaaa gcaagcagag ttgccatagc aacaccaatc
      301 aatgcatatt cgattgcagt aacaccacgt tcatccttcg cgaaacgcgc aaagcactgt
      361 ttagttttac catagtactt agtaataaaa gtattcatgt tttcctcgta tattattatt
      421 taaattcaaa ccaccagctt catctgcgtg tcaccggtat cccatatttt tgggatgtgg
      481 ctataattat ctattcccgc tatttagtct tcaaaaaaaa ttacatttaa tatcatttat
      541 cttgattaag aaatagtaag actaaaaaaa caagcgtcgt cagccagcac actgctaaca
      601 cggtaagaac gctaataaaa acactccact gcttcgttct ataccattca atatttttgt
      661 cagtatgttt tattgggagc agctcttcag tagagaattc ctggctgtta tcattctcag
      721 gaatgtttgc atcagtgttt acttccctgg cctcgctatc gttggtatag ctactactgt
      781 cttcatcact gtcacgctca ttctcacaga catgtttctc gacctttccc agatatctga
      841 cgccttttcg cggaatggtt ttaattagag aactcgacaa acctgcgacg tctaatgcct
      901 ttctaagcat gtaaatctgc ccataataac tgctgtcgct taccgaacct ttctgctccc
      961 gccaaacctc attgataata tgttgcttat cggattctcc c
//
LOCUS       DAJTRH010000271          998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTRH010000271 DAJTRH010000000
VERSION     DAJTRH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            162..446
                     /locus_tag="OO339_004190"
     CDS             162..446
                     /locus_tag="OO339_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5928557.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            434..919
                     /locus_tag="OO339_004191"
     CDS             434..919
                     /locus_tag="OO339_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5928558.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          269 a          208 c          241 g          280 t
ORIGIN      
        1 tccgtgggag cgtagggggg cggtgcccct gcgccacccg atatcccctt tgagtgcggt
       61 tacgtccaca gaggtaaatg ccgctgacgc ggcatctgta atctgttact ttgtgtatat
      121 catttgctat atattgtcaa tatgctattg ttgtaaagca aatgatatac ggaggcttta
      181 tgaaatcagg tgttcagctc aatctcagag caagagaatc acagcggata ctcattgatg
      241 ctgctgcaga aattcttcat aagtcgcgta cagatttcat tctggaaatg gcctgtaaag
      301 ctgctgaaga tgttattctc gaccggcggg tgtttaattt taatgacagg caatatgaag
      361 agttcattga aatgcttgat gctccggttg cagatgatcc tgccatcgaa aaattgctgg
      421 caaggaaacc tcaatgggat gtgtaactgc accagaacct ttatcctcat ttcatcaggt
      481 cgctgaattt gtcagcagtg aagctgtact tgatgactgg ttaaagcaga aggagctaaa
      541 aaatcaggca atcggagcca cccggacttt tgttgtatgc agaaaaggta cgcaacaaat
      601 agtcggtttt tactcactgg ccactggtag tgtcaatcat acagaagcta caggtaacct
      661 tcggcgcaat atgccagacc ccattcctgt aattatactt gcacgcctgg ctgtagatgt
      721 gtcatttcga ggaaaagggc ttggcgcgga tttacttcat gatgcagttc gtcgttgtta
      781 cagggttgcc gaaaatatcg gtgtgcgtgc aattatggtt catgcgctta cggaaaacgc
      841 aaaacagttt tatattcatc atggttttaa gccatcaaaa actcaggtgc aaacattgtt
      901 tcttaaactc cctcaatagg gagtaatgcc gatcagttaa ggatcagttg accgatccag
      961 tggctgtgta agaatccgga aacgctcact cgtttccg
//
LOCUS       DAJTRH010000272          981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTRH010000272 DAJTRH010000000
VERSION     DAJTRH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>981
                     /locus_tag="OO339_004192"
     CDS             <1..>981
                     /locus_tag="OO339_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5928559.1"
                     /translation="SAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGEL
                     SSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWA
                     SLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAADRQL
                     FSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQ
                     NEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSRLSV
                     DPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNV"
BASE COUNT          277 a          234 c          236 g          234 t
ORIGIN      
        1 tccgcgggtg aagagcaaaa gctacaaatt attgccgtat cgaataaagg cgatttaagt
       61 ttccctgcgc agcaaaaatc acttatcgcg ctggcaaata aagcagttag cggaagcggc
      121 gttggcgaac tctcctcttc cagcctgaaa cgctggagtg cggaaaactc ggtaaccatg
      181 agcagtaaag tcagtggcat gaatacgttg ctctccgtta gcgcgcggac taataaccct
      241 gaacctggtt tccagttgat taaccagcga atcacccaca gcacgattaa cgacaatatt
      301 tgggcatcgc tacaaaatgc tcaaattcag gcgttgaaaa cgctcgacca gcgtccagcg
      361 gagaaattcg cccagctgat gtatgagacg cgctatgctg atgaccgcac gaaattactg
      421 caagaaaatc agattgcaca gtttactgcc gcagatgcgc tggctgccga tcgccagttg
      481 ttttcatctc cagcggatat cacgtttgtc attgtcggta atgtcgcaga agacaaactc
      541 gtggcgttaa ttacgcgtta cttaggctca atcaaacact ctgattcgcc attagccgca
      601 ggtaaaccat taactcgcgc gacggacaac gcatcggtta ctgtaaaaga acaaaatgaa
      661 cctgtggcac aggtttcaca gtggaagcgt tatgattccc ggacacctgt taatctggcg
      721 acgcgtatgg cgctcgatgc ttttaacgtc gcactggcaa aagatctacg cgttaatatt
      781 cgtgaacagg catctggggc atacagcgtt tcttctcgcc tctcggttga tcctcaggct
      841 aaagatatca gtcatttgct ggcttttact tgtcaaccag aacgacatga tgaacagtta
      901 acgttagcga atgaagtgat ggttaagcgt ttgactaagg ggatcagtga gcaagaactg
      961 aacgaatacc agcaaaacgt t
//
LOCUS       DAJTRH010000273          980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTRH010000273 DAJTRH010000000
VERSION     DAJTRH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            78..143
                     /locus_tag="OO339_004193"
                     /pseudo
     CDS             78..143
                     /locus_tag="OO339_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            371..619
                     /locus_tag="OO339_004194"
     CDS             371..619
                     /locus_tag="OO339_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5928560.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          308 a          224 c          213 g          235 t
ORIGIN      
        1 aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac tgggacctcg
       61 cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg ctttgtgata
      121 ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga agatcgtcca
      181 ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac gaacttcgac
      241 gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata aacaatatca
      301 ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac tacagttaca
      361 ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta aaatgccaaa
      421 cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac gttatgacga
      481 tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacgc gcaccgccga
      541 taaagattca gctaaaactt ttgttcagga aactctgaaa gatacctggg agtctgctga
      601 cgagtggttt gttcactaat tagcacgtaa aatcagtaac ggctggaaat cattcaatac
      661 tcgcactatc ggaagttcac cagccagccg cagcacgttc ttgcatacga cgtgtctgcg
      721 gtctcaactc cgcccgcccc cgccaatcat gattgggcag taccagtaca gcactagcaa
      781 agacaggaaa ttagcagtct cagcaaaaca ccgaccagac ggtgagaaga caaaaagata
      841 cgcaaagatg ccgcggttcc agaagaataa aaaaccgcta atggacagcc tccttaacac
      901 agttttttat atacatgatc cactgatacc agtggcatta atagctatta tatagcttca
      961 gctccttttt tcatacctta
//
LOCUS       DAJTRH010000274          977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTRH010000274 DAJTRH010000000
VERSION     DAJTRH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.282"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..569
                     /gene="ldhA"
                     /locus_tag="OO339_004195"
     CDS             <1..569
                     /gene="ldhA"
                     /locus_tag="OO339_004195"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5928561.1"
                     /translation="MYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAAALELG
                     VEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFDQMKNGVMIVNTSRGALIDSQAA
                     IEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTA
                     EALTSISQTTLQNLSNLEKGETCPNELV"
     gene            680..>977
                     /gene="hslJ"
                     /locus_tag="OO339_004196"
     CDS             680..>977
                     /gene="hslJ"
                     /locus_tag="OO339_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5928562.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLN"
BASE COUNT          263 a          228 c          247 g          239 t
ORIGIN      
        1 ctatgtatgg caaaacggca ggcgttatcg gtaccggtaa aatcggtgtg gcgatgctgc
       61 gcattctgaa aggttttggt atgcgtctgc tggcgttcga tccgtatcca agtgcagcgg
      121 cgctggaact cggtgtggag tatgtcgatc tgccaaccct gttctctgaa tcagacgtta
      181 tctctctgca ctgcccgctg acaccggaaa actaccatct gttgaacgaa gccgccttcg
      241 atcagatgaa aaatggcgtg atgatcgtca ataccagtcg cggtgcattg attgattctc
      301 aggcggcaat tgaagcgctg aaaaatcaga aaattggttc gttgggtatg gacgtctatg
      361 agaacgaacg cgatctgttc tttgaagata aatccaacga cgtgatccag gatgacgtat
      421 tccgtcgcct gtctgcctgc cacaacgtgc tgtttaccgg gcaccaggca ttcctgacag
      481 cagaagctct gaccagtatt tctcagacta cgctgcaaaa cttaagcaat ctggaaaaag
      541 gtgaaacctg cccgaacgaa ctggtttaat cttgcagctc ccctgcaatc caggggagct
      601 gattcagata atccccaatg acctttcatt ctctattctt aaaatagtcc tgagtcagaa
      661 actgtaattg agaaccacaa tgaagaaagt agccgcgttt gttgcgctaa gcctgctgat
      721 ggcgggatgt gtaagtaatg acaaaattgc tgttacgcca gaacagctac agcatcatcg
      781 ctttgtgctg gaaagcgtaa acggtaagcc cgtgaccagc gataaaaatc cgccagaaat
      841 cagctttggt gaaaaaatga tgatttccgg cagcatgtgt aaccgcttta gcggtgaagg
      901 caaactgtct aatggtgaac tgacagccaa agggctggca atgacccgta tgatgtgcgc
      961 taacccgcag cttaatg
//
LOCUS       DAJTRH010000275          972 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTRH010000275 DAJTRH010000000
VERSION     DAJTRH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            160..963
                     /gene="dkgB"
                     /locus_tag="OO339_004197"
     CDS             160..963
                     /gene="dkgB"
                     /locus_tag="OO339_004197"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5928563.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          280 a          217 c          219 g          256 t
ORIGIN      
        1 tattaagaag cctcgagtta acgctcgatg ttttttgcgt ctggatatct attattgcta
       61 gaatcgcaaa aatcctctgc gttttacgct cttttcccgc aacagtctga agcccataat
      121 cacctcagtt aacgaaaata gcattaaaag aggcatatta tggctatccc tgcatttggt
      181 ttaggtactt tccgtctgaa agacgacgtt gttatttcat ctgtgaaaac ggcgcttgaa
      241 cttggttatc gcgcaattga taccgcacaa atctatgata acgaagccgc agtaggtcag
      301 gcgattgcag aaagtggcgt gccacgtcat gaactctaca tcaccactaa aatctggatt
      361 gaaaatctca gcaaagacaa attgatcccg agtctgaaag agagcctgca aaaattgcgt
      421 accgattatg ttgatctgac gctaatccac tggccgtcac caaacgatga agtctctgtt
      481 gaagagttta tgcaggcgct gctggaagcc aaaaaacaag ggctgacgcg tgagatcggt
      541 atttccaact tcacgatccc attgatggaa aaagtgattg ctgctgttgg cgctgaaaac
      601 atcgctacca accagattga actctctcct tatctgcaaa accgtaaagt ggttgcctgg
      661 gctaaacagc acggtattca tatcacttcc tatatgacgc tggcttatgg taaggcactg
      721 aaagatgagg ttattgctcg tattgcagct aaacacaatg cgattccggc acaagtgatt
      781 ctggcgtggg ctatggggga aggttactca gtaattcctt cttctactaa acgtaaaaac
      841 ctggaaagta atcttaaggc acaaaattta cagcttgatg ccgaagataa aaaagcgatc
      901 gccgcactgg attgcaacga ccgcctggtt agcccggaag gtctggctcc tgaatgggat
      961 taagcctctc tg
//
LOCUS       DAJTRH010000276          959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTRH010000276 DAJTRH010000000
VERSION     DAJTRH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(93..257)
                     /locus_tag="OO339_004198"
     CDS             complement(93..257)
                     /locus_tag="OO339_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928564.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
BASE COUNT          229 a          208 c          321 g          201 t
ORIGIN      
        1 accccgggta ttttcgattt gcgaaatgga tggaacgact tgctcagggg atagctgatg
       61 gagttactga agtaccgaag gaccactaaa aatcacaggc cgggaatttc ggcgtttccg
      121 gcgtaggtgt gccacgaatg catccagtgg cgtgatgttg gatgcaatgc tgtatgagag
      181 atgaggggcg gcgtccagac gcagccttca aaccgccttg agctgctgcc gttaagagcg
      241 gtggtggtga gcgtcaaggt taaaaggcgc agaacatgtg tcaggtaggt gttgcggaga
      301 tgaacgaaag tgaactgtcg atgaagtgtc gaaaggaacc tctaggcgat gtcgaaaatg
      361 tgctatctac gcagcgcatg acgagtccag cagatatctg gtactggctg ggcggcatcc
      421 ggcatatagg cagcatgaac gcaacacagg ctctcgtacg gaacgtggga acctgtcgtc
      481 tcgatgctaa gggagcggcg cggagcagag gcactgcaag cgcctgagta ccgatgcgag
      541 acacaggggc agatggcttc gtagtagtga ggaagtgcct gtaatgggca tggagcgaag
      601 ggggtcagtc gttggggctg gtttatcggt caaccggata gccgggagga gcccatgagt
      661 cagccaaagc cgttcgaggt ctctaagtat gcggtttgga aagcatacca gcgggtcaaa
      721 gctaatcgag gtgcggcagg tgtcgatggg cagtcagtcg aggcgtttga ggttaccaat
      781 cgaagctata cccggtaatg gcgcaggttg acctgcactt ggccaagtgg attgcccgca
      841 aacacaagcg agcgcgaggt agtctggtgc gtgcattcga gtggttactg cgaattcgtc
      901 acgactgccc gacgctgttt gcacactggt gcttagctta cgatacttga atggttcaa
//
LOCUS       DAJTRH010000277          947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTRH010000277 DAJTRH010000000
VERSION     DAJTRH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            29..820
                     /gene="ltrA"
                     /locus_tag="OO339_004199"
                     /pseudo
     CDS             29..820
                     /gene="ltrA"
                     /locus_tag="OO339_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          243 a          217 c          287 g          200 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tggggggtgg aagtcgatat
      541 taaaggcttc gttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tctcggatat gacttcaaag
      841 tgcgtactct gaagaatttc aaaggcgaat tgtacagaaa atgtatgccg ggtgcatcaa
      901 atgcagcaat gtgcaaaata acagaaacaa tcaagaagtg gcgtata
//
LOCUS       DAJTRH010000278          939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTRH010000278 DAJTRH010000000
VERSION     DAJTRH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>939
                     /locus_tag="OO339_004200"
     CDS             <1..>939
                     /locus_tag="OO339_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5928565.1"
                     /translation="YGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGI
                     ASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQE
                     KITKDKDIVFTNGGNVLFKDNLDFGCGGIIFDEGHEYNINGQRFTFKGAGIDIGKESI
                     VNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKV
                     ILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTKKEAVLA
                     INNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILD"
BASE COUNT          376 a          139 c          180 g          244 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggat
//
LOCUS       DAJTRH010000279          932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTRH010000279 DAJTRH010000000
VERSION     DAJTRH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..226
                     /locus_tag="OO339_004201"
     CDS             <1..226
                     /locus_tag="OO339_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR5928566.1"
                     /translation="VLKKRIRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDIT
                     DGLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            424..>932
                     /gene="mcbR"
                     /locus_tag="OO339_004202"
     CDS             424..>932
                     /gene="mcbR"
                     /locus_tag="OO339_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5928567.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYL"
BASE COUNT          279 a          186 c          231 g          236 t
ORIGIN      
        1 ggtgctgaaa aaacgtattc gcttgcaagg gtttattatc gctcaggatt atggtcaccg
       61 catccatgag tttcagaagg agatggggca atgggtgaaa gaggataaaa tccactaccg
      121 cgaagatatt actgatggtt tagagaatgc gccacagacg tttatcggcc tgctgaaggg
      181 taaaaacttc ggcaaagtgg tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg
      241 cgtaagccgc cataaaaatc caggcaaaaa gcttattttc agctttaatt aactaaccac
      301 gtcattgttc tgctgctaat aacaacaaag gtgggtggtt tatgattatt cactttaata
      361 cactaggtga actccttctg ccatgcaggc agggttggac agaaaacacg cgaaggaatt
      421 aaaatgcctg gaacgggaaa aatgaaacat gtcagtttga ctctgcaggt tgagaacgac
      481 ctgaaacatc agcttagtat tggcgcatta aaacctggcg cacgcctgat tactaaaaat
      541 ctggcggagc aattaggtat gagtattaca cctgtgcgtg aagcattatt acgtctggtt
      601 tcggtgaatg cgctttctgt cgcacctgca caagcattta cagtaccgga agtgaggcaa
      661 agtcagttag acgaaatcaa tcggatccgc tacgaactgg aattaatggc agttgccctg
      721 gctgttgaaa acctctcctc gcaagacata gcagaacttc aggaattgct cgggaaatta
      781 cagcaggcgc aagaaaaggg cgacatggaa caaatcatta atgtgaacag gctatttcgc
      841 ttagcgattt atcatcgctc aaatatgccc atcctgtgtg agatgattga gcaactgtgg
      901 atcaggatgg gacctgcttt acattatctt ta
//
LOCUS       DAJTRH010000280          926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTRH010000280 DAJTRH010000000
VERSION     DAJTRH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            144..641
                     /locus_tag="OO339_004203"
                     /pseudo
     CDS             144..641
                     /locus_tag="OO339_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="OO339_004204"
                     /pseudo
     CDS             682..873
                     /locus_tag="OO339_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          241 a          232 c          263 g          190 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agccca
//
LOCUS       DAJTRH010000281          915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTRH010000281 DAJTRH010000000
VERSION     DAJTRH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..496
                     /locus_tag="OO339_004205"
     CDS             <1..496
                     /locus_tag="OO339_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5928568.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
     gene            518..>915
                     /locus_tag="OO339_004206"
     CDS             518..>915
                     /locus_tag="OO339_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5928569.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT          199 a          207 c          300 g          209 t
ORIGIN      
        1 tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta acgtgaaata
       61 ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga
      121 agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg tgcgtaaccg
      181 ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
      241 gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac
      301 tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa
      361 cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga
      421 ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt
      481 cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg
      541 ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg
      601 taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt
      661 ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga
      721 gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga
      781 tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa
      841 ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg cgcaggcgca
      901 gcagagtgcc ggggc
//
LOCUS       DAJTRH010000282          906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTRH010000282 DAJTRH010000000
VERSION     DAJTRH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..610
                     /locus_tag="OO339_004207"
     CDS             <1..610
                     /locus_tag="OO339_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5928570.1"
                     /translation="NRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQA
                     KSIADELMTGCTNFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESY
                     DNGKSGEKFLQELCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNIN
                     HAAMNTLLGERVMDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            625..>906
                     /locus_tag="OO339_004208"
     CDS             625..>906
                     /locus_tag="OO339_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5928571.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT          224 a          198 c          279 g          205 t
ORIGIN      
        1 caaccgcctg aatcgtcagt tgcgggtgga aaaaatcctg aaccgctccg gcatccagcc
       61 gttgcaccgg aagtgttcgt ttgcgaatta ccgggcgcag aacgacggtc agcgacatgc
      121 actgagtcag gcgaaatcca tagctgacga actgatgaca ggctgtacga attttgtgtt
      181 cagcggtaag cccggcaccg gaaaaaatca tcttgcagcc gcgattggca accggctgat
      241 ggcgaagggg cgtagcgtga ttatcgtcac cgtgtccgat gtcatgagcg tgttgcatga
      301 gagctacgac aacggcaaat ccggtgaaaa atttttacag gagctttgtg gtgttgacct
      361 gctggtcctg gatgaaattg gcatgcagcg ggatacgaaa aacgagcagg tggtactgaa
      421 ccagattgtt gatcgccgga cggcatcgtt acgcggtgtg gggatgctga caaatattaa
      481 ccatgcagcg atgaatacac ttctcggcga gcgggtgatg gatcgcatgg tcatgaacgg
      541 cgggcgctgg gtgaatttta actgggagag ctggcgtccg aatgttagcc attcgagggt
      601 tgttaagtag tttcaggagg atttatggcg aaacctttta ctcccgaaca gcgggaagaa
      661 ctgaagacgc gaattgtgga actcgtgcat caggacggtc gggtcacgat tcggcagttg
      721 tccgatgaaa caggtatcag tcgtgcgtct gtcggtcgct tatgcataga actggtcgca
      781 agtggtgatg tatataattc tggctacggc ttattcccgt ctgaacaggc tcgcaaggac
      841 tggcaaagcg cccgcaaaaa actctcgaga gtaaaggtga ggaaaccggt tgttgttgat
      901 ccggac
//
LOCUS       DAJTRH010000283          898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTRH010000283 DAJTRH010000000
VERSION     DAJTRH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            102..>898
                     /locus_tag="OO339_004209"
     CDS             102..>898
                     /locus_tag="OO339_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5928572.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLT"
BASE COUNT          305 a          216 c          129 g          248 t
ORIGIN      
        1 tccagaagtt cctgtctact gctgaaggga accaataaca ttgcaaagca gataaaaaag
       61 aatccaacac ctctcaacat aaagaataca ccctgatatt tatgctcccg acaaataaca
      121 atcacagatt aatttcaaat tcgttctcca cttattcaat cgacactagc cgcgcatatg
      181 aaaattatct aacccattgg actgaatgga aaaataaccg catacaagaa gaacaacgag
      241 acatcgcttt tcagcgacta gtatcatgtc tacaaaacca agagacgaac ctggacttgt
      301 ctgaattagg cctgacaaca ttacctgaaa tccccccggg aattaaatca attaatataa
      361 gtaaaaataa tttaagctta atctccccat tgcctgcgtc ccttacacaa cttaatgtca
      421 gctataacag acttattgaa ctgcctgctt tgcctcaagg acttaaatta ttgaatgcgt
      481 cccacaatca actaatcaca ctacccacac tccccatatc tttgaaggag cttcatgtct
      541 caaataatca attatgttct cttcctgttt taccagaact actggaaaca ttagatgtat
      601 catgtaatgg gctggcagtt ttaccacctt taccattttc tttacaagag attagcgcaa
      661 tagggaatct tcttagtgaa ctcccccctc tacctcacaa cattcactcc atatgggcaa
      721 tcgacaatat gttaaccgat attccatacc tgccggaaaa tttaaggaac ggttattttg
      781 acataaatca gataagtcat atcccggaaa gcattcttaa tctgaggaat gaatgttcaa
      841 tagatattag tgataaccca ttgtcatccc atgctctgca atccctgcaa agattaac
//
LOCUS       DAJTRH010000284          886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTRH010000284 DAJTRH010000000
VERSION     DAJTRH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..583)
                     /gene="ospI"
                     /locus_tag="OO339_004210"
     CDS             complement(<1..583)
                     /gene="ospI"
                     /locus_tag="OO339_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5928573.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSD"
     gene            complement(736..886)
                     /locus_tag="OO339_004211"
                     /pseudo
     CDS             complement(736..886)
                     /locus_tag="OO339_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          270 a          217 c          130 g          269 t
ORIGIN      
        1 gatcagatac atgaattacc tgaaccgaac tattttcatc tacaaatgca tctggcgatt
       61 tttctacagg atcataaaga ttaaatccct ttcctgtttg aacatccaaa aacataattt
      121 tttcaccatc gttaaatcca gctatccaat gagtggcctg ttcaatagaa atcattacta
      181 cacttccttt tgaagtattt tgtaaaatat tttctgcaat acgctcacga aatttactat
      241 aagtttcatt aggatgtgac actatgcctt cactatcaat ttgctctaaa tgcctatgaa
      301 aaaatccggt catatactct gatatttcat taggtacggg agcctctctt actccatacc
      361 ctaacatagc catacaagca tgaagcgcac accctgaaca gttaccatcc ccaccagacg
      421 gatttatcat tttctgtaaa gtcgctgcca attcagaatc tttatccata tcaccaagac
      481 ttttagagtt taaagattcc agcaattttt taacatttac atttccctcc tctgtatgtg
      541 cctcatatcc agcagttccc tgtaacgaca ccccattaat catattgtat cctgacagta
      601 tatatactca ctatattttc ttcataacaa atatccctct gatatgacaa caaaaaacgg
      661 agccggattc ctgtttttgt gaactgcagc tattcgtgtc taaaaagtga tccacttacc
      721 accaacggcc taatatatac agaacggagt aaacaggact aacagttccc gacaggtctc
      781 atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgaata
      841 ccagagccag tgttgttgca gccttgctgc caacgaagcg ccactg
//
LOCUS       DAJTRH010000285          875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTRH010000285 DAJTRH010000000
VERSION     DAJTRH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>875
                     /locus_tag="OO339_004212"
     CDS             <1..>875
                     /locus_tag="OO339_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5928574.1"
                     /translation="DGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCMSYAQY
                     GISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSRLGEHR
                     CRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQ
                     YLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNVKIGDQ
                     VASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVID
                     TFGLKRLSETKRHKN"
BASE COUNT          222 a          180 c          261 g          212 t
ORIGIN      
        1 ggacgggaag aaagtcatta tggcagaagg tgattgcgaa ccttgcctgt tcggatggca
       61 ggctctggac gctggcgtga gggtggttgt gctttgcgaa ggcgaaattg attgtatgag
      121 ctatgcgcaa tacggcatct cggcgttatc cgtgccgttt ggtggcggga aaggcgctaa
      181 gcaacagtgg attgagtttg agtatcacaa cctcgacagg tttgaggaaa tattcatctc
      241 gatggacgtt gatgatgttg gtcgtgaagc cgcaagggaa atcgtaagcc gactcggtga
      301 acatcgttgc cgtcttgtta ctctgccgta caaagacatc aacgaatgcc tgatgaacgg
      361 tgttaccgag gatgaaatct ggcagtacat cggcacggca tcctacttcg atcctgaaga
      421 actctacagt gcgcgagagt tttaccagga cactatcaac gctttctacg gcaagcagca
      481 gtatctgttt aatccaccgt gggaatctct ggcagataaa ttccagttcc gtgaggcaga
      541 gttgacgctg gtcaatggtg tgcacggtca cggaaaggca tgcccactga atgagcctat
      601 tcttttagct gatgggacat ggactactca cgggaatgta aaaattggcg atcaggtggc
      661 gtcagtagac ggcaatccgt caactgtcac tgggatattc ccgcagggtg ttagagatgt
      721 ttaccgagtc acatttgaag atggtcgtta tgttgattgc gcaggcgatc acctatggga
      781 ggtcactagt cgtggattca cgaaaggcga gaaacgccgc gtgatagata ccttcgggtt
      841 gaagcggttg agtgaaacga agaggcacaa aaatg
//
LOCUS       DAJTRH010000286          865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTRH010000286 DAJTRH010000000
VERSION     DAJTRH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..865
                     /locus_tag="OO339_004213"
                     /pseudo
     CDS             1..865
                     /locus_tag="OO339_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          200 a          224 c          257 g          184 t
ORIGIN      
        1 caacaaatga gttcgtcaga gcgcgaaagc gaactggccg ctgaaaatgt ccgccttaaa
       61 cgacaactgg cggagcaggc tgaggaactg tccatcctcc aaaaggccgc gacatacttc
      121 gcgaagcgcc tgaaatgaag tatgtcttta tcgaaaatca tcgggcagag ttcagcatca
      181 aagcgatgtg tcgtgtactt cgggttgccc gcagcggctg gtatgtctgg ctcaggcgtc
      241 gtcaccagat gagcctgcgc caacagtttc ggctcacctg cgatgccgct gtccataagg
      301 cattctttga ggcaaagcag cgatacggtg ctccccgcct tgctgacgaa ctgccggagt
      361 tcaatattaa aaccattgcc gccagcctgc gtcgtcaggg gctgcgggcg aaagccagcc
      421 ggaagttcag cccggtcagc taccgtgcac atggcctgcc cgtattggag aatctgctgg
      481 agcaggactt cagcgccagt ggcccgaaac cagaagtggg cgggtgacat cacgtacttg
      541 cgtaccgatg agggctggtt gtatctcgca gtagtcatcg acctgtggtc acgcgccgtc
      601 attggctggt cgatgtcacc gcgaatgaca gcacaactgg cctgtgatgc actgcaaatg
      661 gcgttgtggc ggagaagacg cccggaaagc gtcattgttc atacggaccg tggtggtcaa
      721 tactgttcag gggattatca ggcaatgctg aagcgacaca acctgcgtgg cagtatgagt
      781 gcgaaaggct actgttatga caatgcctgt gtggaaagct tctttcattc gctgaaggtg
      841 gaatgtatcc acggggaacg cttta
//
LOCUS       DAJTRH010000287          860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTRH010000287 DAJTRH010000000
VERSION     DAJTRH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..750)
                     /locus_tag="OO339_004214"
     CDS             complement(<1..750)
                     /locus_tag="OO339_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5928575.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFR"
BASE COUNT          243 a          224 c          178 g          215 t
ORIGIN      
        1 acgaaaacat tcctggttct cggcaggcgt cggatcttcc atccagaaca ggcggtaatc
       61 ctcaatgctc ttcccaaagc gcgccgcttc gatgggggtc aggcgatggt gcatgtcatg
      121 caacagatgt tcgttaaaac cgaacttatt acgcaccgct tcgaataact tcggcgtaaa
      181 atcgagatac ttttctgaag accacaactg ctcttctggc cacaggcctt tggttgccgg
      241 ttcatacgcc agtcctttgc ccttcgccag gccataagtg gtttgcatac ccggtacacc
      301 gcactgcaca cgaatcgcct taaagcccat ctgctggtgc tttgcatagt cttcaaggac
      361 ttcatcaatc gtacgcccgg tggtgtggca ataaaccatc acgccttcac gagaagcacc
      421 accaagcagt tgatacaatg gcatatttgc cgctttagct ttgatatccc acaacgccat
      481 atcaatggca gaaatggctg acatcgtgac ggggccacgt cgccagtaag cacctttata
      541 aaagaattgc cagatatctt cgatacggct ggcatcgcgg ccaattagtt gtggacaaag
      601 atgatctttc agataagaag cgaccgacaa ttcgcgacca tttaacgtgg catctcctaa
      661 tccacaaagt ccgctttcag tggttatttt aagcgtaacg aaattacgcc ctgggcaagt
      721 aacaaaaaca tctgcagcaa taattttcat tattgcaatc tcctgtgttg taaatagacg
      781 tttcaaacct aattttacgc ctactaccat acaagagaga aaaagcgatt attcaaccgt
      841 gatcacatta aaaacaaaaa
//
LOCUS       DAJTRH010000288          856 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTRH010000288 DAJTRH010000000
VERSION     DAJTRH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..106
                     /locus_tag="OO339_004215"
     CDS             <1..106
                     /locus_tag="OO339_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5928576.1"
                     /translation="TLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            482..589
                     /gene="ldrD"
                     /locus_tag="OO339_004216"
     CDS             482..589
                     /gene="ldrD"
                     /locus_tag="OO339_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR5928577.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          215 a          195 c          267 g          179 t
ORIGIN      
        1 gacgctcgca gagctgggca tggccttctg gcatgattta gcggctccgg tcattgctgg
       61 cattcttgcc agtatgatcg tgaactggct gaacaagcgg aagtagcgtg tcatgtgggc
      121 gtcaggctgc cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg
      181 cctttttcgt aagaaaaatg caacggttgc aagcggataa tattctgcgg aatgcgcagc
      241 acagagtgaa gatgagacgg attccggaac agaaaaacag tcgtagaatc agcgtcaagc
      301 cggaaggatt ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg cattggttgc
      361 tggagagaga aaacccccgc acgttgcagg tatgcacctg acaacaccac gggggccatt
      421 cttgcatcca aacaatacaa gaatagccgc gaaacgttgt cattacaata caggcggcta
      481 tatgacgttc gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc
      541 tggtattctt gccagtatga tcgtgaactg gctgaacaag cggaagtaac gtgccatgcg
      601 ggcgtcaggc tgccgtaatg gcaatttgcg cccggaccag gccgcagggg tgaaactctg
      661 cggccttttt cgtaagaaaa atgcaacgat tgcacgcgga taatattctg cggaatgcgc
      721 agcacagagt gaagacgtga cggattccgg aacagaaaaa cagtcgtaga atcagcggca
      781 agccggaaag gttccggtga ggcgcaatgt tgcgggggct ttatccctgg tggcattggt
      841 tgctggagag agaaaa
//
LOCUS       DAJTRH010000289          855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTRH010000289 DAJTRH010000000
VERSION     DAJTRH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..189)
                     /locus_tag="OO339_004217"
     CDS             complement(<1..189)
                     /locus_tag="OO339_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5928578.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEM"
     gene            197..>855
                     /locus_tag="OO339_004218"
     CDS             197..>855
                     /locus_tag="OO339_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5928579.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT          238 a          203 c          209 g          205 t
ORIGIN      
        1 catttcaacc agttcagctg caaaatcagc acatacattt gaaaagtaag tgcgtagttt
       61 ttctgtcgtg tcatggtcaa aaatctggcg tcggtatttg gtgacaaaga ccaggtgaac
      121 atgcatcagg aaaacacaat gccttccacg ccggatatcg gtttcttttt tcacagacca
      181 aagtatcatt tgacctgtga aacgattaca ggcatttaaa ttccagttaa gacccggtgg
      241 tcaacaggag cgtgaaatga ggcgcttcgc aggcgcatgt cgtttcgttt tcaatcgtgc
      301 tctggcgctt cagaatgaga atcatgaggc cggtaataaa tacatccctt acgggaaaat
      361 ggcttcctgg ctggttgagt ggaaaaacgc cactgaaacg caatggctta aagattcgcc
      421 ctcacagcca ttgcaacagt cactgaaaga ccttgagcgg gcttacaaaa acttcttcca
      481 gaagcgggct gcttttcccc gattcaaaaa gcggggacag aatgctgcat tccgctaccc
      541 gcaggacgtt aagctcgatc aggaaaacag ccgtattttt ctgccgaaac ttggctggat
      601 gcgctaccgg aatagccgtc aggtcacggg agttgtgaaa aatgtcactg tcagccagtc
      661 ctgcggtacg tggtacatca gtattcagac agaaagtgaa gtatcaactc cggttcaccc
      721 ttcagcatca atgatcgggc tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg
      781 cacagtcttt gagcctgtaa acagttttca aaaaaaccag aagacgctgg cgagacttca
      841 gcgacagtta agccg
//
LOCUS       DAJTRH010000290          852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTRH010000290 DAJTRH010000000
VERSION     DAJTRH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            102..449
                     /locus_tag="OO339_004219"
                     /pseudo
     CDS             102..449
                     /locus_tag="OO339_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
BASE COUNT          241 a          207 c          192 g          212 t
ORIGIN      
        1 ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac tttttatacc
       61 ccgcaggggt cataacatgc ttgacaactt aataggggca ctcccccgct aaagtgttgc
      121 ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat atcgaaatca
      181 tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg cgccgccgag
      241 gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa tccaccgccc
      301 gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt agtgccgcca
      361 gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc cgtgtgcgta
      421 gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat ggtcacatcc
      481 taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact ttaactgcgt
      541 gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt ctttagaata
      601 atcaaagcgt tggattgcca tgtcatatga aaatcggcaa agttcatatg taacaaagtg
      661 tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca tgccccaaaa
      721 aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa tactgtataa
      781 aaacacagtg cagagggatt tttatgcgtt ttgaaatctg catagccaaa gagaaaatga
      841 ctaaaatgcc aa
//
LOCUS       DAJTRH010000291          847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTRH010000291 DAJTRH010000000
VERSION     DAJTRH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..824
                     /locus_tag="OO339_004220"
     rRNA            <1..824
                     /locus_tag="OO339_004220"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          207 a          203 c          260 g          177 t
ORIGIN      
        1 ccggtggcga aggcggcccc ctggacgaag actgacgctc aggtgcgaaa gcgtggggag
       61 caaacaggat tagataccct ggtagtccac gccgtaaacg atgtcgactt ggaggttgtg
      121 cccttgaggc gtggcttccg gagctaacgc gttaagtcga ccgcctgggg agtacggccg
      181 caaggttaaa actcaaatga attgacgggg gcccgcacaa gcggtggagc atgtggttta
      241 attcgatgca acgcgaagaa ccttacctgg tcttgacatc cacggaagtt ttcagagatg
      301 agaatgtgcc ttcgggaacc gtgagacagg tgctgcatgg ctgtcgtcag ctcgtgttgt
      361 gaaatgttgg gttaagtccc gcaacgagcg caacccttat cctttgttgc cagcggtccg
      421 gccgggaact caaaggagac tgccagtgat aaactggagg aaggtgggga tgacgtcaag
      481 tcatcatggc ccttacgacc agggctacac acgtgctaca atggcgcata caaagagaag
      541 cgacctcgcg agagcaagcg gacctcataa agtgcgtcgt agtccggatt ggagtctgca
      601 actcgactcc atgaagtcgg aatcgctagt aatcgtggat cagaatgcca cggtgaatac
      661 gttcccgggc cttgtacaca ccgcccgtca caccatggga gtgggttgca aaagaagtag
      721 gtagcttaac cttcgggagg gcgcttacca ctttgtgatt catgactggg gtgaagtcgt
      781 aacaaggtaa ccgtagggga acctgcggtt ggatcacctc cttaccttaa agaagcgttc
      841 tttgtag
//
LOCUS       DAJTRH010000292          846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTRH010000292 DAJTRH010000000
VERSION     DAJTRH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..446
                     /locus_tag="OO339_004221"
     CDS             <1..446
                     /locus_tag="OO339_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5928580.1"
                     /translation="PDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDA
                     SNSSPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFD
                     EVASASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(448..674)
                     /locus_tag="OO339_004222"
                     /pseudo
     CDS             complement(448..674)
                     /locus_tag="OO339_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          213 a          214 c          184 g          235 t
ORIGIN      
        1 tcccggatgc aatcaccttc accggcatct gttactcaca tcgcgtcttt gttgcgttga
       61 atgaaaagcc taatgtaaca gcaatacttt gtggtggtac gtatcgtgcc agaagtgatg
      121 ctttttacga tgccagtaac tcttcgccat tagactctct caatccgcga aaaatattta
      181 tttccgccag cggtgtgcat aatcactttg gcgtcagctg gtttaaccct gaagatcttg
      241 ccactaagcg taaagtgatg aaccgtggac tacggaaaat tttgctcgcc cgccacgcgt
      301 tgttcgatga agtggcctct gccagcctcg caccgatctc tgcatttgac gttctgatta
      361 gcgatcgtcc gttaccggca gattatgtta cgcactgcca gaatggttct gtaaagatca
      421 ttacacctga ttcagaagac gaatgactta ctgaaaaaac accacaatct tgttaaacat
      481 cgtcgggttg gactgattac gttgcacttt caccacatat tccagcttat ctatttggct
      541 tatcacctac tccagacgct ggtcatcctt gaccagtagc cagatatggc ttttgtcgca
      601 gtcctgaatc ggcaggcaga gaatgtcttc aacgttaaga gcgcgacggg caaaagccca
      661 caaaggtgag tcattacacc cggatggtaa acaggcactt ccttgttgta gaagccgtta
      721 tattgtccat aatgtctggg tttttaatgc atactgggta acaaccggac gaatcttcga
      781 atatcactgg cgctgccagc cggtttgaat tcatctcaca accctgcata gagcgaatct
      841 cctgcc
//
LOCUS       DAJTRH010000293          842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTRH010000293 DAJTRH010000000
VERSION     DAJTRH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..166
                     /locus_tag="OO339_004223"
     CDS             <1..166
                     /locus_tag="OO339_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5928581.1"
                     /translation="RVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLV
                     SYSDKPLGLF"
     gene            complement(385..528)
                     /locus_tag="OO339_004224"
     CDS             complement(385..528)
                     /locus_tag="OO339_004224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928582.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            711..>842
                     /locus_tag="OO339_004225"
     CDS             711..>842
                     /locus_tag="OO339_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5928583.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          228 a          176 c          188 g          250 t
ORIGIN      
        1 gcgcgtgtgg accaactgtt atcacgctta cccggcacat gctgcgtttg gtggctacaa
       61 acaatcaggt atcggtcgcg aaacccacaa gatgatgctg gagcattacc agcaaaccaa
      121 gtgcctgctg gtgagctact cggataaacc gttggggctg ttctgatata agaagctggt
      181 cgcattgggt attcattgcc tgatgcgacg cttacgcgtc ttatcatgcc tacgggaacc
      241 tgaccgtagg ccggataagg cgtacacgcc gcatccggca taaacaaagc gcacgttgtt
      301 acaatttacg ttttaaacct acgtgcgcaa taacccagac tacgccaaca gaagtgcgtt
      361 tctggggcgt gttttgtcag gagtttaatc tcgcgtttcg atggtgagcg ggcgaaccag
      421 gccgtaaatc accattggtg atccgaggcc aatcagcaat aaacggtaga taattgaagg
      481 tttaatacgc ttaatctgta ctgtggatgt tttactggag ttattcatta gtttggccaa
      541 tagttccact atttcaatat gataaacctc gtcctgttga aaaaccagac agcacatttc
      601 agaaagtata taaaaaacaa tatgtcgtta tataaataag gaaattgcac tgcatcatgg
      661 catttcataa tatgcgttaa ttgttctttt ttatattctt tgagggaaat atgttcctgg
      721 actattttgc actgggagtg cttatttttg tatttctggt gatattctat gggattatta
      781 ttttacatga tattccctac ctgattgcta aaaaacgtaa tcatcctcat gccgacgcca
      841 tt
//
LOCUS       DAJTRH010000294          824 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTRH010000294 DAJTRH010000000
VERSION     DAJTRH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..253)
                     /locus_tag="OO339_004226"
     CDS             complement(<1..253)
                     /locus_tag="OO339_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5928584.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLE"
     gene            391..>824
                     /gene="pgrR"
                     /locus_tag="OO339_004227"
     CDS             391..>824
                     /gene="pgrR"
                     /locus_tag="OO339_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5928585.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          206 a          185 c          194 g          239 t
ORIGIN      
        1 tttctaactg acgcggctcg ccgccacttt caccttgata agatgcgtca taagcaatag
       61 tgacaaatcc cttttccgcc agcttttcgg cataggttcc ggccgtttgc tctttaacgc
      121 ccccacctgg gtgagatacc acaattgccg gatactgacg ggtttcatca aattttggag
      181 ggaaatagat cactgcagac aaagagatgg ttggattatt gctgttagtg aagctgactt
      241 tattattcat cattcagttc ctcatggagt tgtcggttcg ttttgacggt tggtgatatc
      301 actatagata ttgatcatta agttgattag accctaaatc atgattagac ctatcattta
      361 aatgattaat agattgtatg gagatactga atgaagcgtg aagaaatcgc tgatctgatg
      421 gcgtttgtcg tcgttgcaga ggagcgtagc ttcactcgcg cagcagcccg cctgagcatg
      481 gtgcagtcag ctttaagcca gatagtgcgc cgtatagaag aacgattggg attgcggctt
      541 ctgacgcgaa ccacgcgcag cgttgtaccc actgaagcgg gcgagcatct tttgtctgtt
      601 cttggcccta tattgcatga catagattct gccctgacat ccctgagcga tctgcagaac
      661 cgcccatccg ggacaatacg tattactact gtagaacatg cagcaaaaac gatattgtta
      721 ccagccatgc gcacattcct gaaatcgcat cctgaaattg atattcagct taccattgat
      781 tatggtttga ccgatgtcgt ttctgaacgt tttgatgcag gcgt
//
LOCUS       DAJTRH010000295          799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTRH010000295 DAJTRH010000000
VERSION     DAJTRH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            117..>799
                     /gene="curA"
                     /locus_tag="OO339_004228"
     CDS             117..>799
                     /gene="curA"
                     /locus_tag="OO339_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5928586.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGG"
BASE COUNT          182 a          174 c          260 g          183 t
ORIGIN      
        1 ggattcctcg atgaaattat tgcgtgttgc agaggggatg tgacggctgc aaacaaaacc
       61 aatcccgtga gcctgctact attgatggat tcatgccaaa agcacaggag agcattatgg
      121 ggcaacaaaa gcagcgtaat cgacgttggg ttctggcctc gcgtccacat ggcgcaccgg
      181 ttccggagaa tttccgtctt gaagaagatg atgtcgccac accgggtgaa ggacaggtgt
      241 tactgcgcac agtttatttg tccctggacc cgtatatgcg tggacgtatg agcgatgagc
      301 catcttattc accgccggtt gatattggcg gcgtgatggt cggcggtacg gtgagccgtg
      361 tcgtggagtc gaatcatcct gattatcagc ctggcgactg ggtgctgggc tacagtggat
      421 ggcaggacta tgacatatcc agtggtgatg acctggtgaa acttggcgat catccgcaaa
      481 atccatcgtg gtcgctgggt gtgctgggga tgccaggctt taccgcttat atggggctgc
      541 tggatatcgg tcagcctaaa gagggcgaaa cgttggtggt agctgcggcg acagaaccag
      601 tgggggcgac ggtggggcaa atcggcaaac ttaaaggttg cagagtggtg ggggtagccg
      661 gtggcgcgga aaaatgccgc catgctaccg aggtgttagg cttcgatgtt tgccttgatc
      721 accacgcgga cgattttgcc gaacaactgg cgaaagcgtg cccaaaaggt attgatatct
      781 attatgaaaa cgtgggcgg
//
LOCUS       DAJTRH010000296          792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTRH010000296 DAJTRH010000000
VERSION     DAJTRH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(233..>792)
                     /locus_tag="OO339_004229"
     CDS             complement(233..>792)
                     /locus_tag="OO339_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928587.1"
                     /translation="RQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAA
                     AIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPM
                     KSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEI
                     GVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          159 a          216 c          196 g          221 t
ORIGIN      
        1 ttccttctgt aaggcccctt cctcgggagg ggctttcccg tttcagccct ttgctgactc
       61 cccgattgtt gatgagcgcc ggacctcgcg caacgctttc ccagaggcag ttttgccttc
      121 cccgagcggt cacgcaaaac gttgcaacga cctgatcatt tgcagttttt agcggaacgc
      181 agatataaaa aatccccggc tttttgtgcc agggacgttc atagctgaaa atttatttta
      241 cgttttcggc gacgggagtt gttgagatta agggtgaagt caatgccgcc agtgaaccgc
      301 ctgaaacgcc aatttcattg agcaaggagt caatcagcgg agcctgtgtg cggtaagaga
      361 gagcggctga taatgcttgt tccgctaggt ttccgccccc cacattaccc gtgtttgcat
      421 cacccgcagc gctgccacta ttcagaccat cgacctgaat aatcttgata ccgtcaatag
      481 atttcatcgg ctcaacggat ttttctatta ccgcaggtag cgcctgcaac aaggccagtt
      541 tgaatttaag gctggtttgt tcatcagaaa gtacgttgat agcatcgttc agcgcacgtt
      601 gtgcttctgc ttccgccaga ccctttttat gtgtagcttc ggctaactca acgatagccg
      661 ccgcctgcat ctctgcggct tctttttctg ctttcgcccg cacggtcagt tcaaccgctt
      721 tggtttctgc atcctgcgcg gcagcgatta gggcaacttg tttagcacga tcggcttctg
      781 cggtctggcg ag
//
LOCUS       DAJTRH010000297          781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTRH010000297 DAJTRH010000000
VERSION     DAJTRH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..145)
                     /locus_tag="OO339_004230"
     CDS             complement(<1..145)
                     /locus_tag="OO339_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5928588.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
     gene            complement(157..>781)
                     /gene="yiaK"
                     /locus_tag="OO339_004231"
     CDS             complement(157..>781)
                     /gene="yiaK"
                     /locus_tag="OO339_004231"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5928589.1"
                     /translation="QAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLIVAIPSTPIT
                     MVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYWKGS
                     GMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRDAKLQRIM
                     DYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
BASE COUNT          179 a          205 c          214 g          183 t
ORIGIN      
        1 tttgcgcata aatattcttg ccgtcgattt cgacaacgcc cggctccagg acgttgaagt
       61 tggtagcgcg cagaaaatcg agcgcctttt caatggcggc gggcaaacgg cacggattgg
      121 gctgcgcgat atgtccaaaa atcatgactt atctcctcat aacgcctgga ttttggccca
      181 cacgctgtca tcaacagtga tgccgttacg gcggttttcg gccagcaggg tagtaaattc
      241 gtggccgggt aagcggatgg cctggttttc gtcagcgcgt tcagcggtag taacgtaatc
      301 catgatacgt tgcagcttgg catcgcgggt gggaccgtcg ataagcttgt ccacttcaat
      361 ggcaataaaa atttgtgaaa tgccgtattc gtcgctgttg tcctgggtga cttcggcaac
      421 cgatgcgccg tcggaaagga gagtagcgat catatccagc acaatcgaca tgccagaacc
      481 tttccagtag cccatcggca aaatgcggcg attcttctcg ataacgccag gttctttggt
      541 caaattgccc tcatcatcaa agccaccatc gaccgggagc tgacggcctg ccaggcggtt
      601 aacttctaac atgccgtaag agaacatcga catcgacata tcgaccatgg tgatcggcgt
      661 ggaaggaatg gcgacgatca gcgggttagt gccgatgcga cactcttttg cgccccacgg
      721 cggcattacg gcgatggagt tggtccagca aatgccaata tagccttttt ccgccgcctg
      781 c
//
LOCUS       DAJTRH010000298          779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTRH010000298 DAJTRH010000000
VERSION     DAJTRH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..228
                     /locus_tag="OO339_004232"
     CDS             <1..228
                     /locus_tag="OO339_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928590.1"
                     /translation="YTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGAL
                     VGFLFMALVGALLGGIAWYTRPRSARCRRYE"
     gene            268..732
                     /locus_tag="OO339_004233"
                     /pseudo
     CDS             268..732
                     /locus_tag="OO339_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304276.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
BASE COUNT          169 a          183 c          239 g          188 t
ORIGIN      
        1 tatacatggt gtggctattt cgtctgggca atggcgcgat acatctgggt gatgagccgg
       61 atccccgatg cccccgtgtc gggatttgaa agtgatttag gatctaccgc ggggaagtgg
      121 ttaggagcat tggtcggatt tctgtttatg gccctggttg gtgccttgtt aggcggcatt
      181 gcatggtaca cccgacctcg ttccgcacgt tgcagacgct atgaataaaa actgctaagg
      241 ttgagactct ttggcaacgg agcgcgtatg gagttacaac actggcaggc atagttcgag
      301 aactggttga aaaaccatca tcaacatcag gacgcggcgc atgacctgtg tcattttcgc
      361 cgtgtctggg ctacggcaca gaaactggcg gcagatgacg atgtcgatat gctggtgatt
      421 ttaaccgcct gttattttca cgatatcgtg agcctggcga aaaatcatcc gcaacggcag
      481 cgttcttcaa tcctggcggc agaagagacg cgtcgcctgc tccgtgaaga gttcgtgcaa
      541 tttccggcgg agaaaattga ggccgtttgt catgccattg ccgctcacag tttcagcgcg
      601 caaatcgccc ccttaacaac ggaagctaaa atcgtccagg atgcggatcg gctggaagcc
      661 ctgggggcga ttggcctggc gcgcgtattt gccgtttcag gggcattgag cttggcgctg
      721 tttgatggcg aagctcggtg ccgaactggc gggggagaat gaaggtatcg atcacaagg
//
LOCUS       DAJTRH010000299          755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTRH010000299 DAJTRH010000000
VERSION     DAJTRH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..221)
                     /locus_tag="OO339_004234"
     CDS             complement(<1..221)
                     /locus_tag="OO339_004234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5928591.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSR"
     gene            complement(354..659)
                     /locus_tag="OO339_004235"
                     /pseudo
     CDS             complement(354..659)
                     /locus_tag="OO339_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          174 a          192 c          183 g          206 t
ORIGIN      
        1 cggcttaact gtcgctgaag tctcgccagc gtcttctggt ttttctgaaa actgtttaca
       61 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
      121 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
      181 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
      241 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgagcg
      301 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
      361 aacgccgagc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
      421 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
      481 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
      541 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
      601 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
      661 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
      721 ttcctgatgc atgctcaccg ggtctttgtt accaa
//
LOCUS       DAJTRH010000300          735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTRH010000300 DAJTRH010000000
VERSION     DAJTRH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>735
                     /locus_tag="OO339_004236"
     CDS             <1..>735
                     /locus_tag="OO339_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5928592.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARR
                     KIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWI
                     QLQRKTLSKHAEMAKAFDYILNHWNAL"
BASE COUNT          196 a          172 c          226 g          141 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcctggccca cgcccgccga
      481 aaaatccatg acgaggatgt gcgccgtccg acagaaatga ctcaggaagc gctcagacgg
      541 atagcagagt tatacgacat agaagcggag atacgtggca gtccggcaga ggaacggctt
      601 gcagtcagaa aagccagaag cgtccagttg atgcagtcgt tgtacgactg gatacagttg
      661 cagaggaaaa cgctgtcgaa acatgcggag atggcgaagg cgttcgacta tatcctgaat
      721 cactggaatg cgctg
//
LOCUS       DAJTRH010000301          724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTRH010000301 DAJTRH010000000
VERSION     DAJTRH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..428
                     /locus_tag="OO339_004237"
     CDS             <1..428
                     /locus_tag="OO339_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928593.1"
                     /translation="LSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAPDKDQEPHRPF
                     RVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIGRCMGYLQVAA
                     EIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            494..>724
                     /locus_tag="OO339_004238"
     CDS             494..>724
                     /locus_tag="OO339_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5928594.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQV"
BASE COUNT          190 a          158 c          199 g          177 t
ORIGIN      
        1 cattgtcaga aaaagaggcg gcttacctgc ggcctgagta cgtcattacg gttaacagtg
       61 ccggagagga agagcataaa agtgatttta ttgcgtctgc accggataaa gaccaggaac
      121 cccatcggcc tttcagggtt tcctgtgaag agggtgagtt cgtcgtttat gagaacggaa
      181 aacctgtccg ggcatcgcat catcactgcc tgaaaattat taatctcgct atcagatgcc
      241 tgaaagacga aaacaccaga gttatgaaac gcattggccg ttgcatgggg tatttgcagg
      301 tggcggcaga gattgaagca cttgccagtg gtgcagacat ggatgctgcg gtgcgggagg
      361 ctcttcttcg tgatttcaat actccccctt taagaaagag cctgatgact ggatccagcc
      421 ggggctgact tatcttaaaa ggcgtatata agttggctcg ttatttgttg ccgataaatc
      481 ctgataaata tccatgagcg caaaaatcaa atacggcctg tcggctgctg ttctggcgct
      541 gattgccgca ggcgcgtctg ctccccagat acttgaccag tttctgaacg aaaaagaggg
      601 taatcacaca acggcatacc gtgatggttc tggcatatgg accatctgtc gtggtgccac
      661 aatggtggat ggtaagcctg tcataccggg aatgaagctg tcgaaggaaa aatgtgacca
      721 ggtt
//
LOCUS       DAJTRH010000302          714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTRH010000302 DAJTRH010000000
VERSION     DAJTRH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..>714)
                     /locus_tag="OO339_004239"
     CDS             complement(<1..>714)
                     /locus_tag="OO339_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5928595.1"
                     /translation="KSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIG
                     GLIIAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSE
                     HSLPGSYCALSGVPDAHVSG"
BASE COUNT          165 a          197 c          182 g          170 t
ORIGIN      
        1 ccagagacat gagcatccgg caccccggac agggcgcaat atgaccccgg taatgaatgc
       61 tctgaacatc ccgtaatgaa aaattgtggg gatgctatat acgttccggt gggagtactg
      121 ggcactgaaa tccccaccgg tcccagtcgt gagcctctgt atgactgccc cctgacaagt
      181 ctgatgactt tatcaccgtc agcttctccc tggtacgcag caataatcag cccgccaatg
      241 tcagggtctc cccatctgtt gcggacagag ctcgccacga ttctgtaaat aatatcttct
      301 gtggttatat ttattttcac ccagccagtc agtctggata tgggccagta gcagtagcgg
      361 gtgtgataaa tggggccgtt aatgccgtaa aaagtaaggg atttggctct gtaccgcggt
      421 tctgttgtct cagggcgtgc atcagtccac cggatgctga gcaccccttc aaaccgtgtg
      481 tcgggtatga tgatgccgta ggggccagca acggaatatt cacctggcag cgcattcctt
      541 acccaggcca ggaaatcact cttagtgtca aaacggataa catcttcagg cagaaaagca
      601 cacccaaagc cgaatgcgcc gggtatcgcc agacggcctt ttgtccggtc gtaaatgtcg
      661 ctctgtgctt ccatcgtggc cgcacttttc agccccagat tatcccggga cttc
//
LOCUS       DAJTRH010000303          714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTRH010000303 DAJTRH010000000
VERSION     DAJTRH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            21..281
                     /locus_tag="OO339_004240"
     CDS             21..281
                     /locus_tag="OO339_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5928596.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            287..>714
                     /locus_tag="OO339_004241"
     CDS             287..>714
                     /locus_tag="OO339_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5928597.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFT"
BASE COUNT          203 a          163 c          184 g          164 t
ORIGIN      
        1 tttttgatcg tattgaagaa atggcatggg ctcgtcacta ccagcagatc gttcgtgaag
       61 aaaaagaaac ggaactggcg gacgacctgg aaaaaggtct gcctcagcac ctgtttgaat
      121 cgctctgcat cgaccatttg caacgccacg gtgccaataa acaggcaatc agccacgcat
      181 ttgatgatga tgttgagttt caggagcgca tggcagaaca catccggtac atggctgaaa
      241 ccattgccca ccaccaggtt gatattgatt tggaggacta aaatctatgg cgctttttca
      301 acgagcaata aacacacagg cctatctaaa agcaggaatt atggggtttg ctggcgacgg
      361 caaaacctac acagcaagcg aactggctat tggtctggta atgcttatgc ggcaacgggg
      421 aattgaagct ggtaaccgcc cggtaatgtt tctggacaca gaaacaggct ctgactgggt
      481 taaaccccgc tttgatgcgg aaaatatcga attatttacg gcaaaaaccc gctcgtttgt
      541 tgatctgctt gaagccatta atgaagcgga aagcagcggt tccgtaatga tcatcgactc
      601 catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa accgtaaacg
      661 aggactggaa ttttcagact gggcgtggct caaacaggag tggcgacgct ttac
//
LOCUS       DAJTRH010000304          697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTRH010000304 DAJTRH010000000
VERSION     DAJTRH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            122..688
                     /locus_tag="OO339_004242"
     CDS             122..688
                     /locus_tag="OO339_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5928598.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
BASE COUNT          243 a          131 c          146 g          177 t
ORIGIN      
        1 aaagctatgc gcatttttat tttaaccagt gcaaatcaaa gaattctgta aaattcttta
       61 tataacagag agattgggca tgacaggtga aaaagaaagc aaaaatgcag agcgcatttt
      121 tatgcccccg gaaataatgc gtttcaagga acgccttgaa gaggcaatga atggagaatc
      181 aagccgggcg tttgcagcta agtgcggact ttctgatggg gttatcagaa actacctgtc
      241 tggcaagaca tacccgtctt tagatcgatt agcacaaata gcctacgcaa cagggcgtcc
      301 aatagagtgg tttatccaaa gtcagagcat gccaatggtt gagcataaaa ataatcaaga
      361 gaataaaaat tctcaacctg accgcagcac attgaaaact aaactgatcg caattgtcga
      421 attgttagag gacaaggaac tgcattctgc gattgagttg ttcagaaaga aaggatttga
      481 agctcttatg cctgagattt tttcttccga tgattccatc caggctgatt tgcaaggtat
      541 ttcgcagcaa accttacaaa cagccaaaat gcttgaatca ttaccaacag atactcgtaa
      601 aaagattttg tcaaagtatg gtatttatga acaggaaggt cttgtagctc cttcacaaga
      661 accacaagat gtaaaaaaag ccgtataaca accatga
//
LOCUS       DAJTRH010000305          695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTRH010000305 DAJTRH010000000
VERSION     DAJTRH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..440
                     /locus_tag="OO339_004243"
     CDS             <1..440
                     /locus_tag="OO339_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="starvation-sensing protein; maybe involved in
                     homoserine lactone degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional D-altronate/D-mannonate
                     dehydratase"
                     /protein_id="HCR5928599.1"
                     /translation="IAVGEVFNSIWDCKQLIEEQLIGYIRTTITHAGGITGMRRIADF
                     ASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPYSWRFEE
                     GYMHPGDEPGLGISFDEKLAAKYPYDPEYLPVARLEDGTLLNW"
     gene            507..>695
                     /locus_tag="OO339_004244"
     CDS             507..>695
                     /locus_tag="OO339_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5928600.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVL"
BASE COUNT          158 a          146 c          194 g          197 t
ORIGIN      
        1 cgattgcggt cggtgaagtg tttaacagta tctgggactg caagcagtta atcgaagagc
       61 agctgattgg ctatattcgc acgacgatca cccatgcagg cggcattacc gggatgcggc
      121 ggattgccga ttttgcatcg ctttatcagg tacgtaccgg ttcgcatggc ccttcagatt
      181 tatcgcctgt ttgtcatgcg gcggcgctgc attttgacct atgggtacca aattttggcg
      241 ttcaggagta catgggctat tccgaacaaa tgcttgaagt tttcccgtac agctggcgct
      301 tcgaggaagg ttatatgcac ccaggcgatg aaccggggct gggtatttct ttcgatgaga
      361 aactggcggc gaaatatccg tatgacccgg agtatctccc cgtcgcacga ctggaagatg
      421 gcacattgtt gaactggtaa taacggttcg cttaattatt ttctgaatag tcatcaaatc
      481 gattaattcc gctcaaggag ctaaaaatgg acgttgatgt gtcaaccagt gttgcgggta
      541 ataaaccgca acggattcgt cgtattcaga cagtgaccct ggttttatta tttatggcgg
      601 ggatcgttaa ttttctcgac cgttcgtcat tgagcgtggc aggcgaagcg attcgtggcg
      661 agttaggatt atcggcgaca gagtttggtg ttttg
//
LOCUS       DAJTRH010000306          684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTRH010000306 DAJTRH010000000
VERSION     DAJTRH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            67..540
                     /locus_tag="OO339_004245"
     CDS             67..540
                     /locus_tag="OO339_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5928601.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          225 a          122 c          143 g          194 t
ORIGIN      
        1 tggctggcta acaccccatt ataaggggta cgaataaagt aacggggggg agatgaaccc
       61 tatgcaatgt cctggttaat atctcagtgt gctcatcagt gcactgataa taaaaagacg
      121 gagacagatg ccatttacga taaagttcgg agttcttatt tgctttcatg tattctgaaa
      181 aaaaacaaaa atgttgggct tattcttcat gcaccatcat ttgtttctgt gtctgaaaaa
      241 atcgccagaa ttgtgatggc aaattactcc cgtaactgga gtaattccga gttggccagt
      301 gcggttttaa tgagtgaatc ttctttaaaa agaagaatgt acaaagaggt cggttctata
      361 agtacatttg tacataaaat aaagcttaca gaagctatca ggaaattacg aaggacaaat
      421 actcccattt ctgtcatatc atctgagtta ggttacagtt caccatcgta tttttctaaa
      481 gtcttcttta aatatttaaa gacataccct caaaacataa ggaagaaaaa tggtcggtaa
      541 tgtcaccaat taggaggact tgcagagccg cttttttacg atactgagat caaacagaca
      601 taatctatgc ggagatttac agcagggcat ggttcacaaa tctgacagcg atgaattatc
      661 ggcactgcgg gcagaaaatg ctcg
//
LOCUS       DAJTRH010000307          683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTRH010000307 DAJTRH010000000
VERSION     DAJTRH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            208..>683
                     /locus_tag="OO339_004246"
     CDS             208..>683
                     /locus_tag="OO339_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5928602.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAEQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT          190 a          139 c          160 g          194 t
ORIGIN      
        1 cttgccaaca atcttgcaaa aaaaatgcga ttgctaaaac aaaagtgcaa tttgcggcaa
       61 tatagttatt ccatgaccgt acaatcttgc attcagtgta aataatcttg tttagctctc
      121 gctaaacgcg gttgtataac cgcaatacac gccgtatttt atccggcata tattgttttt
      181 gcttgctcat ggaataggga ggttgtcatg tatcaacgct gttttgataa tgccagtgaa
      241 acgctgtttg tcgccggtaa aacgccacgg ctttcacgtt ttgcatttag cgatgatcca
      301 aaatgggagt ctggacatca cgttcatgac aatgaaaccg agctgattta cgtcaagaaa
      361 ggggttgcaa ggtttaccat cgattcttcg ttatatgtcg cacatgcgga tgacattgtg
      421 gtgatagaac gcggcaggct gcatgcggtg gcctctgacg ttaacgatcc ggcaacgacg
      481 tgtacctgtg cgctgtacgg ctttcagttt cagggggctg aggaaaatca gttactgcaa
      541 ccgcattctt gtccggtaat tgccgcggag cagggaaaag aagtcataaa aaccttattt
      601 aatgagctaa gtgtgatttt gccgcaaagt aaaaatagcc aaacatcttc gttatgggat
      661 gcattagcct atacattagc aat
//
LOCUS       DAJTRH010000308          681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTRH010000308 DAJTRH010000000
VERSION     DAJTRH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(125..255)
                     /locus_tag="OO339_004247"
                     /pseudo
     CDS             complement(125..255)
                     /locus_tag="OO339_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(253..348)
                     /locus_tag="OO339_004248"
                     /pseudo
     CDS             complement(253..348)
                     /locus_tag="OO339_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          195 a          124 c          163 g          199 t
ORIGIN      
        1 tagtttgtgt aagcatatta cttccttatg ttatattttg gtaaaaaatt tattttttta
       61 cattatagtc ggctaaatga agtttactgt aaaaaaacga tactttttcg ggtggcgctt
      121 ccaatcagta gaatatgtgt ttttcaatga aggagccgat gacttttcgt ggatttccac
      181 ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc ggagggtcag
      241 gttgttacgt tcaagatata gcatgtgctg gatgaagatc ccttttctgg tcacctgttt
      301 atctttcgtg gccgacgggg tgacactgtt aaaatgtaat gaacgcatcc cacacaatta
      361 aacacggagt agtatgatgg ttaaaagggt gaaactggca ggtgtggagc ctcgcagctt
      421 tccggcatca tactgcccaa tacgggatgc ataaagcgtc ctcagactgg agactccaca
      481 aatgaaatat acaccggttg gcgttgatat ggcaaaacat gtcattcaga ttcacttcat
      541 caatgagcac atgtcagcgc cagtgatata agacggtaat ggggatgcgg acgatttcct
      601 ggactctcag aagatgaaac atgtttactg aagaacaaaa aattcgcgct attgagcttt
      661 atttcaaata tggtaagaag c
//
LOCUS       DAJTRH010000309          669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTRH010000309 DAJTRH010000000
VERSION     DAJTRH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..233)
                     /locus_tag="OO339_004249"
     CDS             complement(<1..233)
                     /locus_tag="OO339_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5928603.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCR"
     gene            complement(245..646)
                     /locus_tag="OO339_004250"
     CDS             complement(245..646)
                     /locus_tag="OO339_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5928604.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          160 a          201 c          143 g          165 t
ORIGIN      
        1 ttgcgacaca gccgtaaaat atctgctgtt ttcacaaaca gggatggtaa agagtcttca
       61 atacgcacac cgccggccac cagagaaata ttttcaggat catcaaaaac cccggaaatc
      121 atgatccctt ttagccggcc tgacgtaatg accgctctaa tccccatatt gcgaataatg
      181 gccttgtctg cgagatcaag tgcaacatca aacagattat cgaaatcagc cacgctgcct
      241 cccgttattg cattctggcc aggccacgtt ctgtcattcc ggctgccaca ccggcaaaga
      301 cacggaacgc cattcccggc ggcacaaatg ccagaggttc atcccgcgtg gcatgaagtg
      361 catcggtatg cagcgtcacc agtgccacga ctgtaaccag atcatccata ttctgaatca
      421 cggtctccgg ctgcgctgat atcacctcat tttcatgtcc ggtcagcgca ttttccgggt
      481 gggcagactt atcccggatg tcagtgtcat ccatattggc aagttcctcc tccagctcag
      541 ccacacgctg cgcaatatct gctgcgcttc ccgagatatc cggctcacgt cccagcgatg
      601 ctgccagttc tctcagacgt ttcaggttat cgtctttctt tgccatatct gttcctgtcc
      661 gaataaaaa
//
LOCUS       DAJTRH010000310          668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTRH010000310 DAJTRH010000000
VERSION     DAJTRH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>668
                     /locus_tag="OO339_004251"
     CDS             <1..>668
                     /locus_tag="OO339_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR5928605.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAJTRH010000311          668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTRH010000311 DAJTRH010000000
VERSION     DAJTRH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..159)
                     /locus_tag="OO339_004252"
     CDS             complement(<1..159)
                     /locus_tag="OO339_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928606.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAH"
     gene            complement(163..378)
                     /locus_tag="OO339_004253"
     CDS             complement(163..378)
                     /locus_tag="OO339_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5928607.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          196 a          165 c          134 g          173 t
ORIGIN      
        1 gtgagcgact ttgtggcagt aatcgtaaat cgccgcataa tgctcatcgc ggcgtttgtt
       61 ttcatcttca gaactcacca acgccgacaa ttttttatta agctcagtga tttcattttc
      121 caggtgattg aatcgctgat tcatttcttc atgcttcatt atttactctc cccgtgcagc
      181 cttacgcttg tcttctctta ttttgaaata cagattcgtc agataagtca gaagccccag
      241 aagcagactt cccagcacac caatcgcagc ccactgtgat ggcctgacct gatccaacca
      301 ctgcaaaaac cagtagccgg cactgccggc ggaggtgccg taggcaatgc ccgttgaaat
      361 tttgtccatg gatttcatag cctcacctcc gcaaataacg gatggcgtag ttttacactg
      421 agaaatgaaa gggatttgaa aagaaaaaaa cgcaaaagcg ggcgaaacga tatatacagt
      481 aaggaaagca ctctatccaa caaaccaccc acagttaatc ggaataaaag cagagtgctt
      541 atgaatgatc gcacgcccga aggttagtat ttctgcacag caattttgca aaaaaaggcg
      601 atcattcata acttaaacgt ctttcagtca ctccgggatt tcccatcatc gcagactgaa
      661 agactctg
//
LOCUS       DAJTRH010000312          658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTRH010000312 DAJTRH010000000
VERSION     DAJTRH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..580
                     /gene="ycjM"
                     /locus_tag="OO339_004254"
     CDS             <1..580
                     /gene="ycjM"
                     /locus_tag="OO339_004254"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5928608.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT          201 a          125 c          146 g          186 t
ORIGIN      
        1 agtaaactgg aaaaataatc ccgacggcac gcgcagtccc tatgagatga atgtcaccta
       61 tatgaatgcg ttaagccgcc gcgagagtag cgatgaagaa cgttgcacca ggtttatcct
      121 tgcccatgcg attttgttaa gtttccccgg tgtacccgcg atatatattc aaagtattct
      181 tggctcgcgt aatgattacg caggtgtcga aaaactcgga tataaccgtg cgattaaccg
      241 taaaaaatat cacagtgaag agataacacg agaactgaac gatgaagcta cattaaggca
      301 tgcggtatat catgagttgt cgcgtttaat tacacttcgt cgcagccata acgagtttca
      361 tccggataat aattttacca tcgatacggt taattcatcc gtaatgcgta ttcaaagaag
      421 taacgctgat ggtaattgtc tgactggatt gtttaatgtc agtaaaaata ttcagcatgt
      481 aaatattact gatctgcatg gtcgggatct gattagtgaa gttgatatat tgggtaatga
      541 aataacgctg cgcccctggc aggttatgtg gattaaataa aaaggaacat ctcatgatta
      601 aatcaaaaat cgtgctgtta tcagcactgg tttcctgcgc cctgatttca ggctgtaa
//
LOCUS       DAJTRH010000313          657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTRH010000313 DAJTRH010000000
VERSION     DAJTRH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            24..657
                     /locus_tag="OO339_004255"
                     /pseudo
     CDS             24..657
                     /locus_tag="OO339_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          197 a          164 c          166 g          130 t
ORIGIN      
        1 ctcagaacaa cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg
       61 aatccgctca actggttgtt gaccagaaat acacggtggc agatgccgcc aaagctatgg
      121 atgttggcct ttccacaatg acaagatggg tcaaacaact gcgtgatgag cgtcagggca
      181 aaacaccaaa agcctccccc attaccccgg aacaaattga aatccgtgag ctcaggaaaa
      241 agctacaacg cattgaaatg gagaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt
      301 cagactccct gaacagttct cgataatcgg gaaactcaga gcgcattatc ctgtggtcac
      361 actctgccat gtgttcgggg ttcatcgcag cagctacaga tactggaaaa accgtcctga
      421 aaaaccagac ggcagacggg ctgtattacg cagtcaggta cttgagctac atggcatcag
      481 ccacggttcg gccggagcaa gaagcatcgc cacaatggca acccggagag gctaccagat
      541 gggacgctgg cttgctggca ggctcatgaa agagctgggg ctggtcagct gtcagcagcc
      601 gactcaccgg tataaacgtg gtggtcatga acatgttgct atccctaact accttga
//
LOCUS       DAJTRH010000314          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTRH010000314 DAJTRH010000000
VERSION     DAJTRH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            42..656
                     /locus_tag="OO339_004256"
                     /pseudo
     CDS             42..656
                     /locus_tag="OO339_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          174 a          182 c          175 g          125 t
ORIGIN      
        1 acgcggctaa gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa
       61 ccagtaaaaa accccgtaaa cagcattcgc ctgaatttcg cagtgaagcc ctgaagcttg
      121 ctgaacgcat cggtgttact gccgcagccc gtgaactcag cctgtatgaa tcacagctct
      181 acaactggcg cagtaaacag caaaatcagc agacgtcttc tgaacgtgaa ctggagatgt
      241 ctaccgagat tgcacgtctc aaacgccagc tggcagaacg ggatgaagag ctggctatcc
      301 tccaaaaggc cgcgacatac ttcgcgaagc gcctgaaatg aagtatgtct ttattgaaaa
      361 acatcaggct gagttcagca tcaaagcaat gtgccgcgtg ctccgggtgg cccgcagcgg
      421 ctggtatacg tggtgtcagc ggcggacaag gataagcacg cgtcagcagt tccgccaaca
      481 ctgcgacagc gttgtcctcg cggcttttac ccggtcaaaa cagcgttacg gtgccccacg
      541 cctgacggat gaactgcgtg ctcagggtta cccctttaac gtaaaaaccg tggcggcaag
      601 cctgcgccgt cagggactga gggcaaaggc ctcccggaag ttcagcccgg tcagct
//
LOCUS       DAJTRH010000315          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTRH010000315 DAJTRH010000000
VERSION     DAJTRH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(228..>656)
                     /locus_tag="OO339_004257"
     CDS             complement(228..>656)
                     /locus_tag="OO339_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5928609.1"
                     /translation="MAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVILGSIAMLS
                     QAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGSLLGPSFSA
                     MLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          221 a          158 c          146 g          131 t
ORIGIN      
        1 ctgaacattc ccagccatag acaaatcctt cgccaaaatt aaaggggcat ctgtaaaacg
       61 agggattatg cgcggcaaat aagaagtgcg gaaatagcga aatgcaaaat tataacctgc
      121 tgcacatttt atttttaaac gaataaaatt tggaaaaaag caagtcagat agaagaaaca
      181 aaaaagcaga gtgtgaaaca gtaagttcac actttgcttg aattcattta cacgtgagca
      241 acgggtttcg gcgtatgacc ggcgttgcga agcagcatca gcaaatagat aaacgatacg
      301 ctggcgatca tgataaacaa taaattatcg gagaaattct gcattagcat agcgctaaat
      361 gacgggccaa gcagacttcc cacggtatag ctcaacagta aggcttggtt catcgccacc
      421 agttgatgat gttcaacttt ctcacaagcc caggccatcg ccaccggata tagcgtaaag
      481 ccagcggcac cgaggatgaa taacgctggg gccatcgccg cctggctaag catcgcaata
      541 ctgccgagaa tgacgacaaa gacctgaaca cgcaaaacca gcagtcgacc aaacttatcc
      601 gccagacgtc caatcggcca ttgtccgagg atacccgcac tgaccagtac cgccat
//
LOCUS       DAJTRH010000316          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTRH010000316 DAJTRH010000000
VERSION     DAJTRH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(1..656)
                     /gene="chbR"
                     /locus_tag="OO339_004258"
                     /pseudo
     CDS             complement(1..656)
                     /gene="chbR"
                     /locus_tag="OO339_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
BASE COUNT          194 a          157 c          122 g          183 t
ORIGIN      
        1 tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat
       61 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
      121 ctttatcatg catcttttct accgtacttt tcagccacta cggtacatca tcaatcacct
      181 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
      241 cttccagccc tgtttcgcgg aaatttaatg aagaaatcac tgtttccaca taggtgagaa
      301 aagcgttatt ggtccggtat acctgcgaag caacaaagca ataaggcaac aacggcaggt
      361 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatgcgcgtg gcaccaaact
      421 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
      481 acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
      541 aatagtcgtg ctgatgcagt ccgctgatac tctcagtttt gttatagata aacacatgga
      601 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgtta
//
LOCUS       DAJTRH010000317          655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTRH010000317 DAJTRH010000000
VERSION     DAJTRH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            109..>655
                     /locus_tag="OO339_004259"
     CDS             109..>655
                     /locus_tag="OO339_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5928610.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNR"
BASE COUNT          233 a          119 c          113 g          190 t
ORIGIN      
        1 gggggaagca atacctggag aaagagtaca aagcaaactg tattaaactt aataagttat
       61 attgtatttc actagagttc ttatcgttga taagaaattc tggttataat gattaaatca
      121 acgaatatac aggtaatcgg ttctggtatt atgcatcaaa taaacaacat acactcgtta
      181 actctatttt ctttgcctgt gagtttgagt ccatcttgta atgaatatta tttaaaagtc
      241 tggagtgaat gggaaaagaa cggtacccca ggcgagcaac gcaatatcgc cttcaatagg
      301 ctaaaaatat gtttgcaaaa tcaaggggcg gaattaaatc tatctgagtt agatttaaaa
      361 acattacctg atttaccgcc tcagataaca acactggaaa taagaaaaaa cctattaaca
      421 tatctccctg atttcccacc aatgcttaag gtaatacatg ctcaatttaa tcaactggaa
      481 agcttacctg ccttacccga gacgttagaa gagcttaatg tgggtgataa caagataaaa
      541 gaattaccat atcttcctga aactctaact catttacgga tccataataa ccgattgcat
      601 attctgccac tattgccacc ggagctaaaa ttactgatag tttctggaaa cagat
//
LOCUS       DAJTRH010000318          643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTRH010000318 DAJTRH010000000
VERSION     DAJTRH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..264
                     /locus_tag="OO339_004260"
     CDS             <1..264
                     /locus_tag="OO339_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5928611.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>643
                     /locus_tag="OO339_004261"
     CDS             288..>643
                     /locus_tag="OO339_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5928612.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNPPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          171 a          143 c          180 g          149 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat attattctga
      361 aatcccgggt aaaccccccg gaagtggtga tgaacactgt tgctgatgtg atgacaggaa
      421 acgatggtga atactgtttc catgcgcgga ctggaaaata tggtgtgtat ctgaaacagg
      481 actggcgcaa cgagtacaac gttggcgaca ttgctgtata tgaggactca aagcccggca
      541 cgctgaatga ctttctgatt gctcctgatg agggcgacct gaaaccggat gtcgtcaaac
      601 gctttgagga aatggtggcg caggcgcagc agagcgccgg ggc
//
LOCUS       DAJTRH010000319          642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTRH010000319 DAJTRH010000000
VERSION     DAJTRH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..314
                     /locus_tag="OO339_004262"
     CDS             <1..314
                     /locus_tag="OO339_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5928613.1"
                     /translation="AFIDAQELPAVAVWLSDAQYTGKMTDEDDWQAVLHIAVFVRAQA
                     PDSELDTWMENTIFPAMKDIPVLSGLIDTMIPLGFNYQRDNEMATWAMAEITYQITYT
                     N"
     gene            325..>642
                     /locus_tag="OO339_004263"
     CDS             325..>642
                     /locus_tag="OO339_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5928614.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVC"
BASE COUNT          158 a          153 c          194 g          137 t
ORIGIN      
        1 cggcatttat tgatgcgcag gaactgcccg ccgtggcggt gtggctgagt gatgcgcagt
       61 acaccggaaa aatgacggat gaggacgact ggcaggctgt tctgcatatt gccgttttcg
      121 tccgtgcaca ggcgccggat tctgagctgg atacgtggat ggagaacacc atatttccgg
      181 ccatgaagga tattccggtg ctttccggac tcatcgacac catgatccca ctcggtttta
      241 actatcaacg tgataatgag atggccacct gggcgatggc ggaaatcacg taccagatca
      301 cgtacacgaa ttaaggaggt ggtaatgacc acaccaaatc cactggcaaa gacgaaaggt
      361 gcggggacga cgttctggat gtataccggc aacggcgatg cgtttgcgaa ccctttgtcg
      421 gacactgact ggctgcgtct tgcgatggtg aaggatctgc aacctggcga aatgaccgct
      481 gatgcagaag atgacactta tctcgatgat gaagatgcag actggaaaac gacaacccag
      541 gggcagaaat ccgttggtga tacttcggcg acgctggcct ggcgtccggg tgacagcggg
      601 cagaaaaaac tggttcagtt gttcgactcc ggtgaagtct gc
//
LOCUS       DAJTRH010000320          636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTRH010000320 DAJTRH010000000
VERSION     DAJTRH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            52..>636
                     /locus_tag="OO339_004264"
     CDS             52..>636
                     /locus_tag="OO339_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5928615.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          135 a          172 c          188 g          141 t
ORIGIN      
        1 cccggagtgt ggcacggttc actggccgga cggagtcgcc ccgcccttct gatgctcccc
       61 cgttttgccg atatttttca gcagggaaac cgctggctta actggctgga gaaacaaccg
      121 gaaggggcag tccgtccggt ggtaattgaa tcagtgacaa aaatcatggc ctgcgggacc
      181 acactgatgg gctacacgca gtggtgttgt tcatcaccgg actgttgcca tacaaaaaag
      241 gtctgcttcc ggtgtaaaag ccgctcctgc ccgcactgcg gggtgaaggc tggcgcacag
      301 tggatacagt atctgctgag cctggtcccc tactgcccgt ggcagcatat tgtgttcaca
      361 cttccctgcc agtactggtc cctggtgttc cacaaccggt ggttactggc agagatgagc
      421 cgcattgcag cggatgtgat actggaaatc tgccatcagg cagatgtgga gccggggata
      481 ttcacggtga tccacacatg ggggcgtgac cagcagtggc atccgcatat ccatttatcg
      541 acaactgccg gtggtgtgac gtcgggccac acctggaaaa atcttcattt ttacgcccgt
      601 aaggtgatga gcatgtggcg ctaccggata acacgg
//
LOCUS       DAJTRH010000321          628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTRH010000321 DAJTRH010000000
VERSION     DAJTRH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            352..>628
                     /locus_tag="OO339_004265"
     CDS             352..>628
                     /locus_tag="OO339_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5928616.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQ"
BASE COUNT          225 a          115 c          105 g          183 t
ORIGIN      
        1 aaatatatca gggtttgtta ctaataactt ctgaaaacag aaaaaaatag agcagtaatt
       61 ctatttcaga gaggagtaac tccatctatc ctcgatacgt actgaacgtt atcgtgatat
      121 attcatcatg ctttcttcac cattattcga gtatagggag agacgcaatg accttaaata
      181 cctttttatg gctgaattac cattctcagg aataacagca gactccttcc catagacggc
      241 ttagggggaa agcaataccc ggagaaaggg tacaaagcaa actgttttaa aattaataag
      301 ccatattcta tttaactaga attcttatcg ttgataagaa ttctagttat aatgattaaa
      361 tcaaccaata tacaggcaat cggttctggc attatgcatc aaataaacaa tgtatactcg
      421 ttaactccat tatctttacc tatggaactg actccatctt gtaatgaatt ttatttaaaa
      481 acctggagcg aatgggaaaa gaacggtacc ccaggcgagc aacgcaatat cgccttcaat
      541 aggctgaaaa tatgtttaca aaatcaagag gcagaattaa atttatctga gttagattta
      601 aaaacattac cagatttacc gcctcaga
//
LOCUS       DAJTRH010000322          623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTRH010000322 DAJTRH010000000
VERSION     DAJTRH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            3..>623
                     /locus_tag="OO339_004266"
     CDS             3..>623
                     /locus_tag="OO339_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR5928617.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAE"
BASE COUNT          179 a          159 c          160 g          125 t
ORIGIN      
        1 agatgaaaaa agttattcct gctttagccc ttgttctgct gaccaccgga cttgtcggtt
       61 gcgatcgcgt tgagcctggt aatgttggca tcaaggtaaa caaactgggc gacgataaag
      121 gcgtcggaga agtggtcggt gttgggcgct actggacggg atggaacact gaggtttaca
      181 tcttcccgac cttcaaacaa atgaagacct acgatgagcc attcagcttc cagatgagtg
      241 acggtacaac catcggctat cacatcggcg tggcctacaa agttgatcca tccaaagtta
      301 ccacagtctt tcagacctac cgcaaaggcg tggacgacat taccgacacc gacctgcgcc
      361 agaagatagc cgacgcactc aaccgactgg ccagcaaaat gaccaccgac aaatttatcg
      421 acggcggcaa gtctgagctg ctggatgcag ctcttaaaga cattcaggaa gaaatgacgc
      481 ccatcggtat tcaggtaatg agcctctcat atgtgggtaa gccggagtac ccgcctactg
      541 ttatcgacag cattaatgcc aaagtcacgg cgaaccagaa aaccctgcaa tgcgagcagg
      601 aagtaaaaca gcgcgaagcg gaa
//
LOCUS       DAJTRH010000323          617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTRH010000323 DAJTRH010000000
VERSION     DAJTRH010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..497
                     /locus_tag="OO339_004267"
     CDS             <1..497
                     /locus_tag="OO339_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5928618.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
BASE COUNT          159 a          160 c          160 g          138 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggc
//
LOCUS       DAJTRH010000324          614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTRH010000324 DAJTRH010000000
VERSION     DAJTRH010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>614
                     /gene="btsS"
                     /locus_tag="OO339_004268"
     CDS             <1..>614
                     /gene="btsS"
                     /locus_tag="OO339_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5928619.1"
                     /translation="VGGLVGLTGGLHRYSMGGMTALSCMISTIVEGLLGGLVHSILIR
                     RGRTDKVFNPITAGAVTFVAEMVQMLIILAIARPYEDAVRLVSNIAAPMMVTNTVGAA
                     LFMRILLDKRAMFEKYTSAFSATALKVAASTEGILRQGFNEVNSMKVAQVLYQELDIG
                     AVAITDREKLLAFTGIGDDHHLPGKPISSTYTLKAIETGEVVYA"
BASE COUNT          137 a          150 c          172 g          155 t
ORIGIN      
        1 tcgtcggtgg gctggttggt ctgaccggcg gcttacatcg atattcgatg gggggcatga
       61 ccgcgctaag ttgcatgatc tcaaccatcg ttgaaggatt gctcggcggc ctggtacaca
      121 gcatcctgat ccgtcgcggg cgcactgata aagtctttaa ccccattacc gccggtgccg
      181 tcacgttcgt cgctgaaatg gtgcaaatgc tgatcatcct tgcgatcgcc cgaccttatg
      241 aagatgcggt gcgtctggtg agtaatattg ctgcgccaat gatggtcacc aataccgtcg
      301 gcgcggcgct gtttatgcgt atattgctcg ataaacgcgc gatgtttgaa aaatacactt
      361 cggctttttc tgccactgcg ctgaaagtgg cagcctcgac ggaaggcatt ttgcgacagg
      421 ggtttaacga agtgaacagc atgaaagtgg ctcaggtact gtatcaggag ctggatattg
      481 gtgcagtcgc gattaccgat cgagagaaat tgctggcctt taccggaatt ggtgacgacc
      541 accatttacc cggcaaaccg atttcttcga cttacacctt aaaagcgatt gaaaccggtg
      601 aagtggtcta cgct
//
LOCUS       DAJTRH010000325          585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTRH010000325 DAJTRH010000000
VERSION     DAJTRH010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(120..>585)
                     /locus_tag="OO339_004269"
     CDS             complement(120..>585)
                     /locus_tag="OO339_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="YdeF; uncharacterized member of the major
                     facilitator superfamily (MFS) of transporters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5928620.1"
                     /translation="AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFV
                     IGLVGFIFSGNNLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGW
                     LGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
BASE COUNT          170 a          154 c          135 g          126 t
ORIGIN      
        1 ctccgtttat ggtgtggtgt gtttcctcgc ttaccttaat tttcatctca atgagaaaga
       61 tattagcaga gataacctaa acgaaggctg aattaaattt ttattgtgtt cgacttaaat
      121 caacaaagcg cgggctgccc ccacggtctt gcacgaatcc cttttaacat cagcacccac
      181 gcagcaatga tcaccaacgc taagatgaca aacagcgagg aaggcggcag gctggttagc
      241 actacgccac tcactaatgg gttaatcgcg gcaccaagcc agcctaaaga ctgggcggaa
      301 aaatagctgg ctttcattcc tggcggcgca atatggtcaa tcaacatata ctcgcccggc
      361 gcataaatga tttcaccgac agtaaatacc gcagctgaca taccccacaa tagcaggttg
      421 ttgccggaaa aaataaaacc gaccagtccg atgacgaaac agagggtgcc tgctgtcatc
      481 agcgcgcgga tgttagctgg gttaagtcgg cggcccacgg aatattgcaa cgtaaccacc
      541 atggcagcat tcaccggaag aacaaccgcg accacctttt cggca
//
LOCUS       DAJTRH010000326          575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTRH010000326 DAJTRH010000000
VERSION     DAJTRH010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(1..543)
                     /locus_tag="OO339_004270"
                     /pseudo
     CDS             complement(1..543)
                     /locus_tag="OO339_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          201 a          134 c          141 g           99 t
ORIGIN      
        1 aacgcaataa atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa
       61 aaggccagat caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca
      121 aagtaccaga agaagatttg caatattacg gaagatctca atataaattg tggaaagctt
      181 tcgtagcagc cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc
      241 gaacacaata cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg
      301 tccgtaggtg tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc
      361 gcgatccaga aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt
      421 ttgaaataac caaccaacaa tgccgacaac agcaacaacg gcaaggatct ggaataacca
      481 ggcacgaacc gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgagg
      541 catagaaacc tcggaaagca taacgctgaa gagag
//
LOCUS       DAJTRH010000327          564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTRH010000327 DAJTRH010000000
VERSION     DAJTRH010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT          165 a           92 c          133 g          174 t
ORIGIN      
        1 cggtccgatg gtgcaaacaa ttttggtctt tttcatgaca gtcttagtct ttaagttgag
       61 aaggatggga gaaacttgct ttctgggcgc actgccggaa agcgaatccg gtattacgaa
      121 agtggtggtg cctcgctcta aggataggtg acatcgaaaa agcgtgcaga ggaatgtgtg
      181 cttgtgtctc agcccaacgt gagtgtctgt ttttgagtcg ttgagttcta cagtccaaag
      241 tgctgaaacc attcaagagt caattggcgc gcattatacg ctgaaattaa tccaaaagga
      301 aaacaaaaac agcgtttcaa aaaatataca acttgttaga caataaattg ttatcaaaga
      361 tttaacgttc cagggaaaag gttacgcaaa aacttcgctg tttgcgcaac gtctggtgcc
      421 gaagttgtca gtgtgaggat aagtgaaagt tcatccggtg tataaatata tcatcgctca
      481 attgctacag ttaaaataat ttatcgcgtt atttgtctga ttttgtttga tttttgttta
      541 attggttaga ttgatatatg ctga
//
LOCUS       DAJTRH010000328          564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTRH010000328 DAJTRH010000000
VERSION     DAJTRH010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..107
                     /locus_tag="OO339_004271"
     CDS             <1..107
                     /locus_tag="OO339_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5928621.1"
                     /translation="VDRITPDDGGCSYIRLRREGLPGNVRAGRYYEGA"
     gene            94..>564
                     /locus_tag="OO339_004272"
     CDS             94..>564
                     /locus_tag="OO339_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5928622.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          134 a          134 c          182 g          114 t
ORIGIN      
        1 gtgtggatcg tatcacccct gatgatggcg gatgtagtta tatccggttg cggcgtgagg
       61 ggctgccggg aaacgtaagg gcaggacgat attatgaagg ggcttgagaa tgccatccgc
      121 aatctgaaca gccttgatac ccgtatggtg ccacaggcca gcgcatgggc gataaaccgt
      181 gtggcacaga aagcggtctc ggttgccacc cggcaggttg ccgggaatac cgttgcgggc
      241 gataaccagg tgaaagggat ccccctgaaa ctggtacgtc agcgtgtccg ggtgtttaaa
      301 gccagtccgt caggaaaaat gacggccagg atccgcgtta accggggcaa tccgcccgcc
      361 atcaaactga acacaacacg gcggcgtgct ggtgaaggac tgagagtggg aaaatacttt
      421 ttccgggggg catttgttca gcaactggcg aatggccgct ggcatgttct gaggtgtctt
      481 cctgaagcgc gttttgcaac agggcatgac catcagggca ggctaagaaa aaatcgtctt
      541 cctgtggagg tagtgaaaat cccg
//
LOCUS       DAJTRH010000329          548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTRH010000329 DAJTRH010000000
VERSION     DAJTRH010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..313)
                     /locus_tag="OO339_004273"
     CDS             complement(<1..313)
                     /locus_tag="OO339_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR5928623.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FS"
     gene            complement(352..>548)
                     /locus_tag="OO339_004274"
     CDS             complement(352..>548)
                     /locus_tag="OO339_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5928624.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
BASE COUNT          160 a          133 c          116 g          139 t
ORIGIN      
        1 aactaaacgg caggccaaga ccaattgctg ttttggcatc aacgcccgta gaccatgcaa
       61 tgacggtggt catcagaccc gccataatcg ggttgggcgg ctgaacacca cctgcagggg
      121 ttaatccggc gaaagccaac tctgtcagac caccggtaat taagccagtc tgaatatcac
      181 cgagaatagc gccagttagg gtacaaacga ttatcgggcg gaataaaaat aaggcttcca
      241 gccaaaaatc aatccccaga acgaaaacta acgccgctag ggataatccc tgaagtaggg
      301 ttatttcatg catttttatt acctcaaacc aatattaagg cgattttaga tttagtcagg
      361 gatttgttct ttttgatcgc cagggacgtc ctgaatgaaa acattcacgc cacgttgttt
      421 aataaaacgt aagtccgcga gatcctggtc atcgacataa actttactgc tgatttgctt
      481 tttcccttcc gagaaatgca tattgccgac gttgacatct ttcaggtcaa tgccaccttc
      541 taccaatt
//
LOCUS       DAJTRH010000330          546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTRH010000330 DAJTRH010000000
VERSION     DAJTRH010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..287
                     /locus_tag="OO339_004275"
     CDS             <1..287
                     /locus_tag="OO339_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR5928625.1"
                     /translation="YSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDS
                     VDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(395..>546)
                     /locus_tag="OO339_004276"
     CDS             complement(395..>546)
                     /locus_tag="OO339_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928626.1"
                     /translation="DPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTTTKML
                     FMCRE"
BASE COUNT          180 a          127 c           99 g          140 t
ORIGIN      
        1 agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat gccaaaaaag
       61 gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc ttcgggccag
      121 gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt cacggacaat
      181 actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga aacgacgccc
      241 gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg tcaaccgacg
      301 gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca agaggttaat
      361 gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata aataacatct
      421 ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa taacgatcat
      481 cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta aattcgctgg
      541 gatcgt
//
LOCUS       DAJTRH010000331          539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTRH010000331 DAJTRH010000000
VERSION     DAJTRH010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..539
                     /locus_tag="OO339_004277"
                     /pseudo
     CDS             1..539
                     /locus_tag="OO339_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          125 a          133 c          164 g          117 t
ORIGIN      
        1 tccagcgcct gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat
       61 atggacgggc aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag
      121 cttagtcagc cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac
      181 cgatgactgg atggatggcc gccgcagtcg tcacactgat gatacggatg tgcttctccg
      241 tatacaccat gttatcggag agctgcccac gtatggttat cgtcgggtat gggcgctgct
      301 tcgcagacag gcagaacttg atggtatgcc tgcgatcaat gccaaacgtg tttaccggct
      361 catgcgccag aatgcgctgt tgcttgagcg aaaacctgct gtaccgccat cgaaacgggc
      421 acatacaggc agagtggccg tgaaagaaag caatcagcga tggtgctctg acgggttcga
      481 gttctgctgt gataacggag agagactgcg tgtcacgttc gcgctggact gctgtgatc
//
LOCUS       DAJTRH010000332          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTRH010000332 DAJTRH010000000
VERSION     DAJTRH010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT          148 a          126 c          106 g          145 t
ORIGIN      
        1 ggtgccgtag gcaatgcctg ttgatatttt ttccatctga tacatatccc gcccccccga
       61 cagacctgtg ctatcggaaa gaaaaaaggc catcagcaaa actctgatgg cctgaatcac
      121 ctttaccaat attgtatgaa aaaacacgca cgactaattg acaataattt tcatttccat
      181 taaaaaagat cgcgtagcat tcttaattca tgaggaactt tacccgccag caatctgagt
      241 agcgaaagct gttcatcccc aatgtttttg ctggcgggtc cttttttcta cggtcctcct
      301 ccccggaggg gggcatttca ttatttttct aatcgttact ggtgagaagg cggaagaaaa
      361 agccagttct tcgcccctaa ataacagcat gattccagtc catgcaaaat ctgatttcca
      421 gctcacacat accgagcgct gtacaaaaaa ccgccagcca aggcagttaa gagtgtgttc
      481 ccggggtttg cttagaatat tttgtaagtt gtccgaagag atatt
//
LOCUS       DAJTRH010000333          523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTRH010000333 DAJTRH010000000
VERSION     DAJTRH010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>523
                     /gene="proB"
                     /locus_tag="OO339_004278"
     CDS             <1..>523
                     /gene="proB"
                     /locus_tag="OO339_004278"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5928627.1"
                     /translation="VTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLF
                     SIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDN
                     DNLSALAAILAGADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVS
                     GLGTGGMSTKLQAA"
BASE COUNT          117 a          135 c          156 g          115 t
ORIGIN      
        1 ttgtgacgtc gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag
       61 cgactatcgc ctcgaaacaa ctgctggcgg cggtagggca gagtcgactg attcaactgt
      121 gggaacagct gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg
      181 atatggaaga ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata
      241 acaatatcgt tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaggtcg
      301 gcgataacga taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc
      361 tgctgaccga tcaaaaaggt ttgtataccg ctgatccgcg cagaaatccg caggcagaac
      421 tgattaaaga tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt
      481 caggcctcgg aactggcggc atgagtacta aattgcaggc cgc
//
LOCUS       DAJTRH010000334          515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTRH010000334 DAJTRH010000000
VERSION     DAJTRH010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT          166 a           76 c          103 g          170 t
ORIGIN      
        1 atatttcaga gttggagttc gaatgttgac acctgagtat ttcctgctaa aaatagccct
       61 gctttacgga tggttgtatt gcaataacac gaaagtagta agagattaaa ggtatctgtc
      121 agcgataact ctgcaagtga tgacattttg taaaccaaat aatgtaaatc agcaatgtat
      181 cttttcactt aattataaca aatgtggtat tcactaagaa tctgatacga gcttacatca
      241 cgactatttt agtctcatcg cgggatacag ggatgcaccg atggatctca gcacaccgta
      301 atgcaatacc tgaacaaata gaacagtaaa gtggttcagt attaatgaaa gctcttattt
      361 tccgttggga aaacttaaaa tagatgagaa ggctttttga tttcttatgt gatctcattt
      421 cgagtattga gaaatttgtg atttttaaat ttttcaataa gccctgtaag ggattggggt
      481 tttatgagaa ataaagagac gtattgtctt tgttt
//
LOCUS       DAJTRH010000335          513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTRH010000335 DAJTRH010000000
VERSION     DAJTRH010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>513
                     /locus_tag="OO339_004279"
     CDS             <1..>513
                     /locus_tag="OO339_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5928628.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANS"
BASE COUNT          142 a          104 c          124 g          143 t
ORIGIN      
        1 tgagcgcatt ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc
       61 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
      121 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      181 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      241 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      301 tggtactgac ctgttcatga ccaaccgtac caacggtgtt gcaacctatc gtaacacaga
      361 tttctttggc atggttgaag gtctaaattt tgctctgcaa tatcagggta aaaatgaagg
      421 tacaggcaac tacaaagcaa atggcgatgg ccatggttta tctgcaactt acactattga
      481 cggtttctca tttgctggtg cttatgcaaa ctc
//
LOCUS       DAJTRH010000336          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTRH010000336 DAJTRH010000000
VERSION     DAJTRH010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            210..506
                     /locus_tag="OO339_004280"
     CDS             210..506
                     /locus_tag="OO339_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5928629.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          140 a          112 c          113 g          143 t
ORIGIN      
        1 tttcagtaga aaaactaggc ctcgatttta aatgcaaata gcaatcatat tcaataaggc
       61 aagacgatca ggatcagtga aaatgtcatt tttggcgtaa ttacccttac tttatgcatg
      121 accttaacaa agacatcatc tttccgttgc agatcttcgc cctttcaaat aacgtacttt
      181 acaactttcc cgaacaagga gttgtgcccg tgttgtatgt tatttacgct caagacaaag
      241 ctgatagcct cgaaaaacgc ctttccgttc gtccggcaca tttagcacgt ttacagttac
      301 tgcatgatga aggtcgtttg ttgacagctg gtccaatgcc agcggtagac agtaacgatc
      361 caggggctgc gggatttact ggctcaaccg ttattgctga atttgaatcg ctggaagcag
      421 ctcaggcctg ggccgatgct gacccgtacg tagcggcagg agtctatgaa cacgtttcag
      481 tgaaaccatt taagaaagtg ttctgagt
//
LOCUS       DAJTRH010000337          495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTRH010000337 DAJTRH010000000
VERSION     DAJTRH010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..434
                     /gene="aroD"
                     /locus_tag="OO339_004281"
     CDS             <1..434
                     /gene="aroD"
                     /locus_tag="OO339_004281"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5928630.1"
                     /translation="VDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEE
                     IIARLRKMQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVI
                     SRLVGEVFGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT          134 a          119 c          120 g          122 t
ORIGIN      
        1 tggttgatat gatcgatctg gagttattta ccggcgatga tcaggtcaaa gaaaccgtcg
       61 cctacgccca cgcgcatgat gtgaaagttg tcatgtccaa ccatgacttc cataaaacgc
      121 cggaagccga agaaatcatt gcccgtctgc gcaaaatgca gtccttcgac gccgatattc
      181 ctaagattgc gctgatgccg caaagtacca gcgatgtgct gacgttgctt gccgcgaccc
      241 tggagatgca ggagcagtat gccgatcgtc caatcatcac gatgtcgatg gcaaaaactg
      301 gcgtaatttc tcgtctggtt ggtgaagtat ttggctcggc ggcaactttt ggtgcggtaa
      361 aaaaagcctc tgcgccaggg caaatctcgg taaatgattt gcgcacggta ttaactattt
      421 tacatcaggc ataagcaata atatttcggc ggaaataccc tcccccgccg aaataaaaaa
      481 agtattttta accgt
//
LOCUS       DAJTRH010000338          494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTRH010000338 DAJTRH010000000
VERSION     DAJTRH010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(172..402)
                     /locus_tag="OO339_004282"
                     /pseudo
     CDS             complement(172..402)
                     /locus_tag="OO339_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT          155 a          124 c           94 g          121 t
ORIGIN      
        1 ttatgcaata tattgaattt gcatggtctt gtaggccaga taagacgttc acgtcgcatc
       61 cggcatgaac aaagcgcact ttgtcaaaaa tctaacctac tttttaattc aggaaattac
      121 cgcaaagccc acgccatcat acccaacgta acaaaactca ggcacgttcc ccactcgccc
      181 cgagaaaaat agcattaatg cgcccagcgt cagcataaaa atattgagca gaaggtaaat
      241 tgttggcgta aagagtaaat tgcaaacctc aggaatagcg gcaagcccag cccccagcat
      301 ggcaataacc gctgcaaaaa attgcgtcag tattgggatg ccgaaaaaag tcattaccag
      361 cgaggtcaaa atccattttc tattttgcat tattctttcc attctttctg aatggcgaaa
      421 ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat ggcgaaaaaa
      481 acgggaccct ttgg
//
LOCUS       DAJTRH010000339          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTRH010000339 DAJTRH010000000
VERSION     DAJTRH010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT          177 a           78 c           82 g          152 t
ORIGIN      
        1 gatttaatag cgtttgcttc ggtgatttat tactcattag ttgtttttct aaattaacaa
       61 cagaataact ttcttgttta atgagattaa tattggcaac cattatagcc tccttcccac
      121 ggtttaagag caaacataca acataatgat tcataacata cagagcagta aatgaatgat
      181 actgacaatg tatgccagcc cattatcttt gcataatatt tgtgagctat agattttaaa
      241 aatatttatg aaaaaagtat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac
      301 ctgaactaat atgatgagtt aagcattatc tgcaataatt gccactcagc tctacaaata
      361 atatttaaat gctcctgaac agtgatgtag caagacacgt atggtgccaa cgaagaataa
      421 aaatttataa agttcttatt caatataacc ggaatagtca gggactgatg taaatgaccg
      481 gaatgaatg
//
LOCUS       DAJTRH010000340          487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTRH010000340 DAJTRH010000000
VERSION     DAJTRH010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            316..>487
                     /locus_tag="OO339_004283"
     CDS             316..>487
                     /locus_tag="OO339_004283"
                     /inference="COORDINATES: protein motif:HMM:NF024418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HCR5928631.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNV"
BASE COUNT          172 a           91 c           76 g          148 t
ORIGIN      
        1 agaacttctt cggcacactg caaaatcgca tcgtggtcga tcgctaattc gtcgtgaatc
       61 actgaacgat gttccatgcg aacagatatt cgatcatatc gaaatattaa acattacccc
      121 tccccacatt gggcaaaaaa aaacaaaaaa cgatcaatta gtattgattc acataatctc
      181 tcatcattaa tgagaacatt gtcacatatt tacaatatag aactgttact tttagcaagc
      241 tgttagtaac caaccgttac taataatttt aattcttaat actttgttca ttcaataaat
      301 gagttgagag aatatatgaa taaaatatac tcccttaaat atagtgctgc cactggcgga
      361 ctcattgctg tttctgaatt agcgaaaaga gtttctggta aaacaaaccg aaaacttgta
      421 gcaacaatgt tgtctctggc tgttgcaggt acagtaaatg cagcaaatat tgatatatca
      481 aatgtat
//
LOCUS       DAJTRH010000341          487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTRH010000341 DAJTRH010000000
VERSION     DAJTRH010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>487
                     /locus_tag="OO339_004284"
     CDS             <1..>487
                     /locus_tag="OO339_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5928632.1"
                     /translation="EIWHSFVSGIWALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFV
                     ATVIYREMTFATLWHVLIGAAKTTSVVMFLVASAQVSAWLITIAELPMMVSDLLQPLV
                     DSPRLLFIVIMVAILIVGMVMDLTPTVLILTPVLMPLVKEAGIDPIYFGVMFIINCSI
                     G"
BASE COUNT           87 a          120 c          117 g          163 t
ORIGIN      
        1 aggaaatctg gcactctttt gtctccggta tctgggcgct gtttcttcca gtcatcatta
       61 ttggcgggtt tcgctccggt ctgtttaccc ctaccgaagc cggggccgtt gctgctttct
      121 atgcgctgtt tgtcgccaca gttatttacc gtgaaatgac cttcgccaca ctttggcatg
      181 tactcattgg cgcggcgaaa accacctcag tggttatgtt tctggtggcc tcggcacaag
      241 tttccgcctg gctgattacc attgctgaac tgccgatgat ggtttctgat ttactgcaac
      301 cgttggtcga ttcaccgcgt ctgctgttta tcgtcattat ggtggctatc ctgattgtcg
      361 gcatggtcat ggatttaacg ccgacggtat taattcttac cccggtatta atgcctttag
      421 ttaaagaggc aggaatcgat ccgatttatt tcggtgtcat gtttatcatt aactgctcaa
      481 tcggctt
//
LOCUS       DAJTRH010000342          487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTRH010000342 DAJTRH010000000
VERSION     DAJTRH010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..>487)
                     /gene="aqpZ"
                     /locus_tag="OO339_004285"
     CDS             complement(<1..>487)
                     /gene="aqpZ"
                     /locus_tag="OO339_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5928633.1"
                     /translation="AAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLT
                     MAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKT
                     GFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIA
                     IG"
BASE COUNT          156 a          152 c          106 g           73 t
ORIGIN      
        1 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
       61 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
      121 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
      181 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
      241 gggcaattac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
      301 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
      361 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
      421 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
      481 cagctgc
//
LOCUS       DAJTRH010000343          481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTRH010000343 DAJTRH010000000
VERSION     DAJTRH010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..481
                     /locus_tag="OO339_004286"
                     /pseudo
     CDS             1..481
                     /locus_tag="OO339_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          175 a           81 c           95 g          130 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 a
//
LOCUS       DAJTRH010000344          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTRH010000344 DAJTRH010000000
VERSION     DAJTRH010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>472
                     /locus_tag="OO339_004287"
     CDS             <1..>472
                     /locus_tag="OO339_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5928634.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAJTRH010000345          471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTRH010000345 DAJTRH010000000
VERSION     DAJTRH010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..361
                     /locus_tag="OO339_004288"
     CDS             <1..361
                     /locus_tag="OO339_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5928635.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAJTRH010000346          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTRH010000346 DAJTRH010000000
VERSION     DAJTRH010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT          119 a           94 c           89 g          162 t
ORIGIN      
        1 tttttatctg tggttattta gataacatta tctaactgga cactttcttg tatgtattat
       61 agctaaatga acagatttta atatgttgta tggttttcac tctgaaatcc cctgaattta
      121 atcacgtttc ctttactgaa ggcaatttta accgtagtat caattattaa cccttgggct
      181 tgatatccct ctcctcttct gggcacgggg gatgtcagat tacttattac agaaaccaaa
      241 agaattgtaa gcgtcatctc agtgtcgtct atttcaatgt ctgatgattg gcgatgatag
      301 agtcctctta taacgttaat ggactctatc aatgtcagat tctcttcgcc cccgcaggat
      361 tcgtgcctct tactctatga ctctatggag tttaagcttg atcttattga aaaatcttac
      421 cagctagggg cctgcgtggc ccaactggcc agagagtacg ggat
//
LOCUS       DAJTRH010000347          449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTRH010000347 DAJTRH010000000
VERSION     DAJTRH010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(206..400)
                     /locus_tag="OO339_004289"
     CDS             complement(206..400)
                     /locus_tag="OO339_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5928636.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
BASE COUNT          130 a          104 c           85 g          130 t
ORIGIN      
        1 aaaagacggg atttgatggg attcaataag ttatgcgccg attgcattca agataaaacg
       61 agttaaaaat gctcaatgct tcgatatcga taaaacattc aagataggcg cgccgtgtat
      121 tttaattctt ttaagactct tcaaaaatgt catcgtagcc tttaaaaacg aattttaaag
      181 gcaccctaaa ggcaggtcaa tgaagttaac ccgccttcgc gtttacactc tctgagagaa
      241 tactagcttc aatctcttcc aaaatctctc tgacttgctc gtcgggcata tcaacgagca
      301 tcaacgctgc caggcgttta cggccctcta gttgcagtag ctcttgagtt tccttatcta
      361 acaagcgtag cacagtctca acacctttcc ttgcgagcat gtttgtaagg gcttctcgtt
      421 cctcactggt aagaaacgac aacatttct
//
LOCUS       DAJTRH010000348          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTRH010000348 DAJTRH010000000
VERSION     DAJTRH010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>447
                     /locus_tag="OO339_004290"
     CDS             <1..>447
                     /locus_tag="OO339_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5928637.1"
                     /translation="NLASIGGIAIWGWIISIIGAMSLAYVYARLATKNPQQGGPIAYA
                     GEISPAFGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVF
                     TFVNMLGGTWVSRLTTIGLVLVLIPVVMTAIVGWHWFDAATYAANWN"
BASE COUNT           91 a           95 c          109 g          152 t
ORIGIN      
        1 aacctagcaa gtatcggtgg tattgctatc tggggttgga ttatctctat tattggtgca
       61 atgtcgctgg cgtatgtata tgcccgactg gcaacaaaaa acccgcaaca aggtggccca
      121 attgcttatg ccggagaaat ttcccctgca tttggttttc agacaggtgt tctttattac
      181 catgctaact ggattggtaa cctggcgatt ggtattaccg ctgtatctta tctttccacc
      241 ttcttcccag tattaaatga tcctgttccg gcgggtatcg cctgtattgc tatcgtctgg
      301 gtatttacct ttgtaaatat gctcggcggt acctgggtaa gccgtttaac cactattggt
      361 ctggtgctgg ttcttattcc tgtggtgatg actgctattg ttggctggca ttggtttgat
      421 gcggcaactt atgcagctaa ctggaat
//
LOCUS       DAJTRH010000349          441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTRH010000349 DAJTRH010000000
VERSION     DAJTRH010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(64..441)
                     /locus_tag="OO339_004291"
                     /pseudo
     CDS             complement(64..441)
                     /locus_tag="OO339_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          114 a          115 c          102 g          110 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcattttaca gccccgtccg ggctgtatgt tttcaccagc ttatccagtg atcgttttga
      121 aaatggctgc attggatcac gtggctgcaa aaatacataa tccctgttgc actcaggaac
      181 tgaatcacgc caggctttct gctcgtcgat aatccgccgg atctcaggcg ttatcggcag
      241 gcggaaagcc ttttgtgttt tcatagcccc tcgcatgccg ataacccctt ccggataaac
      301 aatttcacca gcctcctcgt aaacgtagtc ccagcgcagg ttatggacat taatcggacg
      361 aacaccagtg atgatcatga agcgaacagc attcttctgg tgtacagagg tgcaggcagc
      421 aacattgagc cagagtcggg c
//
LOCUS       DAJTRH010000350          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTRH010000350 DAJTRH010000000
VERSION     DAJTRH010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..440
                     /locus_tag="OO339_004292"
                     /pseudo
     CDS             1..440
                     /locus_tag="OO339_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          142 a           63 c           85 g          150 t
ORIGIN      
        1 taattaataa tctttatggc agtgagtttc ggataagaga tattaatgag aattttgttg
       61 ggatggattt tacctgtgaa ttccgacccg aaaaaatgac gaatttgatc aatataaaaa
      121 ataggttggt tattcattat ttaaggacta tatactatga aaaaaattat atacatcctg
      181 ccgggagcat ttttgctgcg attcaaaaaa aatgagagtc tcctaaagtt tccttcgata
      241 atatcaatgc ttaatatcaa tcttcttttt aatccactaa atcttccggg aatgggaagt
      301 ggtgtgcttg aagatattat gagtattcca gatagttcac ttagaaaacg tctcgggtat
      361 gaggttttaa gcttctcact acaggcacat tcattgagcc aggaatgtat tgataagttg
      421 gacatttttt tcgctgacga
//
LOCUS       DAJTRH010000351          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTRH010000351 DAJTRH010000000
VERSION     DAJTRH010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>438
                     /locus_tag="OO339_004293"
     CDS             <1..>438
                     /locus_tag="OO339_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5928638.1"
                     /translation="LVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKP
                     GGLGYVGVLAAMIMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAP
                     IIPPSIPFIIFGVSSGLSISKLFMAGIAPGIMMGATLMLTWWR"
BASE COUNT           70 a          120 c          134 g          114 t
ORIGIN      
        1 cgctggtgaa cggagccgat agcttctccc tcctggcgat tccgttcttt gtgctggcgg
       61 gtgaaatcat gaatgcgggc ggcttgttaa aacgcattgt tgacctgccg atgaaactgg
      121 tggggcataa accaggcggg ctgggctacg tcggcgtgct ggcagctatg attatggcga
      181 gcctttctgg ttctgccgtt gcggataccg ccgccgttgc cgctctactg gtgccgatga
      241 tgcgcagcgc caactacccg gtcaaccggg cggcggggct gattgcctct ggcggcatta
      301 tcgcgccaat tattccccct tccattccgt ttattatctt cggtgtttcc agcggattat
      361 ccatcagtaa gctgtttatg gcgggcattg cccctggcat aatgatgggc gcaacgctga
      421 tgcttacctg gtggcggc
//
LOCUS       DAJTRH010000352          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTRH010000352 DAJTRH010000000
VERSION     DAJTRH010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            35..434
                     /locus_tag="OO339_004294"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO339_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTRH010000353          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTRH010000353 DAJTRH010000000
VERSION     DAJTRH010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..305)
                     /locus_tag="OO339_004295"
     CDS             complement(<1..305)
                     /locus_tag="OO339_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5928639.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          124 a           80 c           94 g          136 t
ORIGIN      
        1 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
       61 ttgttaatgg ataaatccat caacttgtgg acgttttcgt cgtcccaggt ctggcagaaa
      121 gtggccaggt tctgacgagc gttgccatca agatataatt catcattgat aatctgaaat
      181 gcgacatcgt cgcgcatttc gtgcagcgga aaacgttttg attctgcgat agtggaaata
      241 gacttcgcac caaaacgtga atcgagtagt tccgacctta aatccgttac ttgcttctta
      301 tccattttaa actccttaaa atgattggat cgcattaaaa aagtaggatt tatcgataaa
      361 gtaagcaagt gcaaaggact cgtgtttaaa taacaaaatc ctaatgttat ttatcgtgag
      421 atattgttgg aatg
//
LOCUS       DAJTRH010000354          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTRH010000354 DAJTRH010000000
VERSION     DAJTRH010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..>433)
                     /locus_tag="OO339_004296"
     CDS             complement(<1..>433)
                     /locus_tag="OO339_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5928640.1"
                     /translation="DNKIKELPFLPENLTHLRVHNNRLHILPLLPPELKLLVVSGNRL
                     DSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNAFVNV
                     AGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSATISAPEH"
BASE COUNT          125 a           73 c           99 g          136 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattt gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc aagcttatct ggaaaggggg gaatgctgtc
      301 taatctgttt ccagaaacta ccagtaattt tagttccggt ggcaatagtg gcagaatatg
      361 caatcggtta ttatgaaccc gtaaatgagt tagattttca ggaagaaatg gtaattcttt
      421 tatcttgtta tca
//
LOCUS       DAJTRH010000355          427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTRH010000355 DAJTRH010000000
VERSION     DAJTRH010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..217
                     /locus_tag="OO339_004297"
     CDS             <1..217
                     /locus_tag="OO339_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5928641.1"
                     /translation="SFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDC
                     RIRSNNCYGQVIRRDQESALACWGIDQ"
     gene            214..>427
                     /locus_tag="OO339_004298"
     CDS             214..>427
                     /locus_tag="OO339_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5928642.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKE"
BASE COUNT          108 a          102 c          116 g          101 t
ORIGIN      
        1 gtcattttgc ccctataaca ttggccccgg taagtgtttc ccgtcgacgt tttataagcg
       61 gctgaatgcc ggtgatcgta agggcgcatg cgaggcgatt cgctggtgga taaaagatgt
      121 tgggcgcgat tgccgcatac gttcaaataa ctgctatgga caggttattc gtcgtgacca
      181 ggaaagcgca ttagcctgtt gggggataga tcaatgagca gagtcaccgc gattatctcc
      241 gctctggtta tctgcatcat cgtctgcctg tcatgggctg ttaatcatta ccgtgataac
      301 gccattacct acaaagagca gcgcgacaaa aacgccggag aactgaagct ggcgaacgca
      361 accattactg acatgcagca gcgccagcgt gatgctgatg cactcgatgc taaatacacg
      421 aaggagt
//
LOCUS       DAJTRH010000356          420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTRH010000356 DAJTRH010000000
VERSION     DAJTRH010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..372
                     /locus_tag="OO339_004299"
                     /pseudo
     CDS             1..372
                     /locus_tag="OO339_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          143 a           86 c           74 g          117 t
ORIGIN      
        1 cttcaaccct gaatacaaag gtgattcatc taagccaata gttctatcgc tgactggttt
       61 aatggtgcaa ctaataaaat tgctaacttc caagaaccga tgaataactt cgtaaataac
      121 acacaatcaa ctccatcagc tatcacaggt gacatggtta atagcaatgc tcaattagct
      181 atggctgtat cagagttagg aagaaaggta gacgctttaa cagcagaaac taagaagcct
      241 ttatcagtta atatcactgg tgaagcaact gttagaccgg atgcaccatc tttctttagc
      301 ttcaatcaat cagctaatga ccaattcacg caggatatgc tcttaagttc aagctatcca
      361 gaggaagaat gatttcttat atatccgtgt gctacgtgaa atattctgaa cagatatacc
//
LOCUS       DAJTRH010000357          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTRH010000357 DAJTRH010000000
VERSION     DAJTRH010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>415
                     /locus_tag="OO339_004300"
     CDS             <1..>415
                     /locus_tag="OO339_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5928643.1"
                     /translation="NIGSMFRNGWENNVKSIDALPHIEAGSNARTSDISSEEYAIMPL
                     APMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITYDAYHMAEVYLTQP
                     MQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGAN"
BASE COUNT          116 a          104 c          109 g           86 t
ORIGIN      
        1 caatattggt tcgatgttcc gcaatggttg ggaaaacaat gtgaaatcta ttgatgctct
       61 gccacacatt gaagctggct caaacgcccg aaccagcgat attagcagcg aagaatacgc
      121 cattatgcca ctggctccaa tgaaggaggc cgatgcgccg aacgaagaac tgcgtcaggc
      181 ctgggagtat taccacacac ctcgtgcgca gtatccaaca gcaccgggtt acgctacttt
      241 gcgcagcctt aaccagatta ttacctatga tgcttaccat atggcggaag tgtacctgac
      301 tcagccgatg cagattgtgg cgggcagcca ggcagggagc aaatggatga gtgacgacct
      361 gtacgatcgg gcatcaagcc aggataaacg ctatcacatt gttgaaggcg caaac
//
LOCUS       DAJTRH010000358          414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTRH010000358 DAJTRH010000000
VERSION     DAJTRH010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            78..>414
                     /locus_tag="OO339_004301"
     CDS             78..>414
                     /locus_tag="OO339_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR5928644.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVA"
BASE COUNT           97 a          112 c          106 g           99 t
ORIGIN      
        1 tcttgtctct ccgacagact gaccgaattg ttatcaatgt taacaaaaaa aagaacaatt
       61 ggttataagg agagagtatg cgtgtcatca ccctggcggg tagtcctcgc tttccttctc
      121 gctccagctc cttgctggaa tatgcgcggg aaaaactaaa tggcctagat gtagaggttt
      181 atcactggaa tctgcaaaac ttcgccccgg aagatctgct ttatgctcgt ttcgatagtc
      241 cggcactcaa gaccttcacc gaacagctgc aacaggctga tgggctgatt gtcgccacgc
      301 ctgtgtataa agccgcctat tccggcgcgc tgaaaaccct gctcgacctg ctgccagagc
      361 gcgctttgca aggcaaagtg gtgctaccgc tggcgacggg cggtaccgtg gccc
//
LOCUS       DAJTRH010000359          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTRH010000359 DAJTRH010000000
VERSION     DAJTRH010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(202..411)
                     /locus_tag="OO339_004302"
     CDS             complement(202..411)
                     /locus_tag="OO339_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5928645.1"
                     /translation="MFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
BASE COUNT          133 a           92 c           82 g          106 t
ORIGIN      
        1 ataaacggta aatgggtgcg aaagttttaa tagcaaaacc gcaatatcgg gataacgcta
       61 caggagaaag cgattgatct attttcctga aacaaggtga atattcaaaa actcctgtca
      121 aattgccttt cgccctgaaa aatgcatagt caaatatctg ttttaactaa ttggcgttgc
      181 agtacatgca acgccaatta gttagcggaa tttacgtcga tactcgcctg gcgtcatccc
      241 aaagcgttgc ttaaataccg ttgaaaaatg actctggtca gaaaatcccc aatgaaagcc
      301 gatgcctgcc agtttttcat catctgcggc atggcgaatc gcatctgcac aaaaatcgag
      361 acgacggtta cgaatatatt gcgcgactac caaaccttta tcggcaaaca ttc
//
LOCUS       DAJTRH010000360          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTRH010000360 DAJTRH010000000
VERSION     DAJTRH010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(50..190)
                     /locus_tag="OO339_004303"
     CDS             complement(50..190)
                     /locus_tag="OO339_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928646.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..>405
                     /locus_tag="OO339_004304"
     CDS             329..>405
                     /locus_tag="OO339_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5928647.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIF"
BASE COUNT          134 a           66 c           64 g          141 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttat
//
LOCUS       DAJTRH010000361          400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTRH010000361 DAJTRH010000000
VERSION     DAJTRH010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..>400)
                     /locus_tag="OO339_004305"
     CDS             complement(<1..>400)
                     /locus_tag="OO339_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5928648.1"
                     /translation="YHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHVVDVYNIGTA
                     ITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNTITVKDSTVT
                     SGSWTDEGTTGWFGNTGNASDYSGKSNFVTV"
BASE COUNT          111 a           87 c           86 g          116 t
ORIGIN      
        1 acggtgacaa agttagattt accactataa tcgctggcat tacctgtatt accgaaccaa
       61 ccagtagtac cttcatcagt ccatgaacca gaggttacgg tagaatcttt aacagtgatg
      121 gtgttattaa acacttcgct gctgtttacg ccagtgctga cagtgttgtc gtcaatatct
      181 tcccactcat aaccctgagt caacgtaata cccgcaacgt gagagttatt agtgatggac
      241 agatcaactt cctgatccag agtaatagcc gtaccaatgt tgtaaacgtc tacaacatga
      301 gtatcagcgg cattattgta agtgccgtta tatgtgtagt gttcgtagtt gtcgtcgatc
      361 gttgaattat caacagtcag cgccagacgg tcatggtagt
//
LOCUS       DAJTRH010000362          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTRH010000362 DAJTRH010000000
VERSION     DAJTRH010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..398
                     /locus_tag="OO339_004306"
                     /pseudo
     CDS             1..398
                     /locus_tag="OO339_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          112 a          114 c           88 g           84 t
ORIGIN      
        1 caccctgacg taaccatcga aaacggtcgc gctgtcacta cgtctgttgc ggttgcagag
       61 ttcttccaca aacgacacga caatgtgtta cgtgccatcg caaatactga atgctcacct
      121 gaatttaacg ccctcaattt tgaggacgtc acctacaccg acaccaaagg cgaaaaacgc
      181 ccaatgtacc aaatcaccaa aaacggcttc gttttcctgg tgatgggctt cactgggaaa
      241 aaagccgctg cattcaaaga ggcctacatc gctgatgttc gaccgcatgg aggcagaact
      301 gcaccagaat aataccccgt ccgctgacaa gatgattccg ggtaacggtc gcactctggt
      361 tgtgcacttc gacgaacacg gcaatatcag attcaccg
//
LOCUS       DAJTRH010000363          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTRH010000363 DAJTRH010000000
VERSION     DAJTRH010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            28..>395
                     /locus_tag="OO339_004307"
     CDS             28..>395
                     /locus_tag="OO339_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928649.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAE"
BASE COUNT          115 a           89 c          100 g           91 t
ORIGIN      
        1 acgcagcgcc atataaaggg acagataatg gaatcgctct cagagagaac ctcaacaggc
       61 taccagcaaa tccacaacgg cattattcat ctggtcgata gcgcccgaac ggaaacggta
      121 cgtagcgtta acgcgctgat gaccgcgacg tactgggaaa ttggccgacg gattgtcgaa
      181 tttgaacaag gtggtgaggc cagagcagct tatggtgcac agttgattga tcggctatcg
      241 aaggatttaa gtcgacggta taaacgtggg gtctctgcaa aaaacttacg ccaaatgagg
      301 cttttttacc tcttttttca acatgttgaa attcgccaga cagtgtctgg cgaattaaca
      361 ccattgggaa ttccgcagac accatctgca gaatt
//
LOCUS       DAJTRH010000364          390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTRH010000364 DAJTRH010000000
VERSION     DAJTRH010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>390
                     /locus_tag="OO339_004308"
     CDS             <1..>390
                     /locus_tag="OO339_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR5928650.1"
                     /translation="VTFDRSDESAKALESGRCDTLASDQPQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSN"
BASE COUNT          104 a           86 c          106 g           94 t
ORIGIN      
        1 gtgactttcg atcgctctga cgaatcagcg aaggcactgg aatctggtcg ctgcgatacg
       61 ctggcttcgg atcagccgca actgtatgcc ttgcgcatca aattaagcaa cccggctgaa
      121 tggattgtct taccggaggt tatctctaaa gaaccgcttg ggccggtagt tcgtcgtggc
      181 gatgatgaat ggttctcgat tgtacgctgg acgcttttcg ccatgctaaa tgctgaagag
      241 atgggcatca attcccagaa cgtcgatgaa aaagcggcta atccagcaac gcctgatatg
      301 gcacatctgt tgggtaaaga aggcgattac ggcaaggatc tgaagttgga taataaatgg
      361 gcctataaca tcatcaaaca ggtgagtaac
//
LOCUS       DAJTRH010000365          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTRH010000365 DAJTRH010000000
VERSION     DAJTRH010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT          133 a           82 c           84 g           88 t
ORIGIN      
        1 acaagcgttt tatcttcaac gggaatgaga tatggaaacg tggagaatca accacagagt
       61 atacccgcgc acaccagcga acaatcagac gactgcgcag actcatcgcc aggaaacagt
      121 atacagaaaa atggttcaga aaatatgaca catggagcgc cggatattac gaactgatgg
      181 caacaaaacc attctgacgt aaacgaaatt aaccatgacg caattaaata aggcaagccg
      241 aatacatcag gaggaccatg aacatttatt tcagaatagt tatatcactg gcaattatcg
      301 catgtattta cggattaatc gttccggccc tcatatcaat gaaggatacg gtagcagtga
      361 tttctggctt tgctctggcg tgtctga
//
LOCUS       DAJTRH010000366          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTRH010000366 DAJTRH010000000
VERSION     DAJTRH010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.374"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..320
                     /locus_tag="OO339_004309"
     CDS             <1..320
                     /locus_tag="OO339_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5928651.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYA
                     SMA"
BASE COUNT          110 a          101 c           84 g           87 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactcca tcaactgccg ttggttgctc
      181 ttcctctccc gtttgacgaa agccagcaaa aaaagctgga agttcctttt accctgctgt
      241 ggcataaacg gaacagccat aatccgaaga tcgtctggtt acgggaaacc attaaaaacc
      301 tttacgcgtc gatggcataa ccgaatcgta tgaaacgcga cccaatttca caataaaatg
      361 taaaaaagtt gtaataagct tg
//
LOCUS       DAJTRH010000367          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTRH010000367 DAJTRH010000000
VERSION     DAJTRH010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>382
                     /locus_tag="OO339_004310"
     CDS             <1..>382
                     /locus_tag="OO339_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5928652.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPAD"
BASE COUNT          102 a           83 c           95 g          102 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ct
//
LOCUS       DAJTRH010000368          381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTRH010000368 DAJTRH010000000
VERSION     DAJTRH010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(273..>381)
                     /locus_tag="OO339_004311"
     CDS             complement(273..>381)
                     /locus_tag="OO339_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5928653.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          106 a           87 c           77 g          111 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt t
//
LOCUS       DAJTRH010000369          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTRH010000369 DAJTRH010000000
VERSION     DAJTRH010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            154..327
                     /locus_tag="OO339_004312"
     CDS             154..327
                     /locus_tag="OO339_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928654.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
BASE COUNT          115 a           76 c           81 g          106 t
ORIGIN      
        1 tgtatgacaa ataaaaccat aatccttgcc catacgtcca tctggcttat ttttaatcaa
       61 ttcacccgat ctttgatctc atcaacggta tcaaaataaa ccagcgtata acgttcaaat
      121 cgcacataag attcgataaa ccatggaggt tatatgaaaa aactggcact tattttgttt
      181 atgggaacgc ttgtttcctt ttatgccgat gccgggcgca aaccctgttc tggttcgaaa
      241 ggggggatct cacactgtac ggcaggcggc aaatttgtct gtaatgatgg ttctattagt
      301 gcatcgaaaa aaacatgcac taactgaagt gtaaaagggg tgccatgaga aaatgaatct
      361 gctagtcaga tgcgcggg
//
LOCUS       DAJTRH010000370          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTRH010000370 DAJTRH010000000
VERSION     DAJTRH010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>374
                     /locus_tag="OO339_004313"
     CDS             <1..>374
                     /locus_tag="OO339_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5928655.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAJTRH010000371          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTRH010000371 DAJTRH010000000
VERSION     DAJTRH010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..159)
                     /locus_tag="OO339_004314"
     CDS             complement(<1..159)
                     /locus_tag="OO339_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5928656.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRT"
     gene            complement(170..370)
                     /gene="cspG"
                     /locus_tag="OO339_004315"
     CDS             complement(170..370)
                     /gene="cspG"
                     /locus_tag="OO339_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5928657.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
BASE COUNT          106 a           84 c           86 g           96 t
ORIGIN      
        1 agtgcgtatt tgcagcgcga cgttatcaaa ggtgatcata gtcgatttac aaaaaataca
       61 cttagcgccc aaagggtttc gctccgtcac atcgaatgcc gatgtacggt actgtgaacc
      121 atgacaacat ggacagcgga aatggagatg ttgagtaata acggcaacct tagagcgtaa
      181 caacgttcgc tgccgcgggg ccacgttgcc cctgctcaat agaaaattca actttctgat
      241 tttcgttcag cgtgcggaat tcattgctct ggatggcggt gaaatggacg aaaacgtctt
      301 tgctgccatc atcaggagtg ataaagccaa aacctttatc tgcgttaaac cattttacta
      361 aaccagtcat tt
//
LOCUS       DAJTRH010000372          366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTRH010000372 DAJTRH010000000
VERSION     DAJTRH010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.380"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            19..>366
                     /locus_tag="OO339_004316"
     CDS             19..>366
                     /locus_tag="OO339_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5928658.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNY"
BASE COUNT          101 a           76 c          105 g           84 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattat
//
LOCUS       DAJTRH010000373          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTRH010000373 DAJTRH010000000
VERSION     DAJTRH010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.381"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>365
                     /locus_tag="OO339_004317"
     CDS             <1..>365
                     /locus_tag="OO339_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5928659.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAJTRH010000374          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTRH010000374 DAJTRH010000000
VERSION     DAJTRH010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.382"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>361
                     /locus_tag="OO339_004318"
     CDS             <1..>361
                     /locus_tag="OO339_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5928660.1"
                     /translation="VSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQVLSGMYPSSVM
                     AASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKV
                     YGEVDSNGIPKKGLLLAA"
BASE COUNT           69 a           89 c          100 g          103 t
ORIGIN      
        1 agtaagtact ggtatggtta aaaacccgaa acgtaccgtt ccgctggcaa ccatgctggg
       61 tactggttta gcaggtattg tttatatcgc tgcgactcag gtgctttccg gtatgtatcc
      121 gtcttctgta atggcggctt ccggtgctcc gtttgcaatc agtgcttcaa ctatcctcgg
      181 taactgggct gcgccgctgg tttctgcatt caccgccttt gcgtgcctga cttctctggg
      241 ctcctggatg atgttggtag gccaggcagg tgtacgtgcc gctaacgacg gtaacttccc
      301 gaaagtttat ggtgaagtcg acagcaacgg tattccgaaa aaaggtctgc tgctggctgc
      361 a
//
LOCUS       DAJTRH010000375          352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTRH010000375 DAJTRH010000000
VERSION     DAJTRH010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.384"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            181..>352
                     /locus_tag="OO339_004319"
     CDS             181..>352
                     /locus_tag="OO339_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5928661.1"
                     /translation="MRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDNL
                     KKINGKNEKLLGG"
BASE COUNT          120 a           70 c           87 g           75 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg ga
//
LOCUS       DAJTRH010000376          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTRH010000376 DAJTRH010000000
VERSION     DAJTRH010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.385"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..341
                     /gene="arsR"
                     /locus_tag="OO339_004320"
     CDS             <1..341
                     /gene="arsR"
                     /locus_tag="OO339_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5928662.1"
                     /translation="PIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISR
                     HLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQN
                     CSGDSKNICS"
BASE COUNT           92 a           85 c           87 g           86 t
ORIGIN      
        1 tacccatcca attgttcaaa attcttgctg atgaaacccg tctgggcatc gttttactgc
       61 ttagcgaact gggagagtta tgcgtctgcg atctctgcac tgccctcgat cagtcgcagc
      121 ccaagatatc ccgccatctg gcattgctgc gtgaaagcgg gctattgctg gaccgcaagc
      181 aaggtaagtg ggttcattac cgcttatcgc cgcatattcc agcatgggcg gcgaaaatta
      241 ttgatgaagc ctggcgatgt gaacaggaaa agattcaggc aattgtccgc aacctggcca
      301 gacaaaactg ttccggagac agtaagaaca tttgcagtta aaaatttagc
//
LOCUS       DAJTRH010000377          348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTRH010000377 DAJTRH010000000
VERSION     DAJTRH010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.386"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..>348)
                     /locus_tag="OO339_004321"
     CDS             complement(<1..>348)
                     /locus_tag="OO339_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928663.1"
                     /translation="AWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAELPDYNFSGDG
                     KDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIGMLCGLLETDG
                     YVEKDGTLVFSSA"
BASE COUNT           93 a           97 c           70 g           88 t
ORIGIN      
        1 ttgctgagga aaaaacaagc gttccatcct tctcgacata cccatccgtt tcaagcagac
       61 cacacagcat gcctatacgc gttgatttat ttgcggaaaa aaacacacga gggatgaatt
      121 tgttttttgc tgtgcacccc attagtccat aacctcgcag ggtctccatg agtggattta
      181 cctgaccacg cgccgttgat atcagccagt ccttaccatc tccagagaag ttgtaatcag
      241 gcagttcagc cttcatacgc tcgatcatgt atggctcgac gtttgaaaac ttcacgctcc
      301 cattgctaag actaccatcc ccgagaaggg agccgataac ccatgcta
//
LOCUS       DAJTRH010000378          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTRH010000378 DAJTRH010000000
VERSION     DAJTRH010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.387"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            84..347
                     /locus_tag="OO339_004322"
                     /pseudo
     CDS             84..347
                     /locus_tag="OO339_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           98 a           71 c           87 g           91 t
ORIGIN      
        1 ccccttgaaa cgccagacag tggatgtatc ttaaaataag agataggctt ggatatatgt
       61 ctaacactag ttctaatttt gagatgactg gaactttgct ggggctggag cttcgtaaac
      121 gtaaaactcc tcaggaaaaa attgccatta ttcagcagac aatggagcct ggcatgacgg
      181 tgtcccatgt ggcgcgtttg catggtattc agcccagcct gttgcttaag tggaaaaaat
      241 aatatctgga aggaagtctc actgctgttg ctgccggaga ggatgtcgtt cctgcctcag
      301 aactggctgc cgctataaaa caaataaaag agttacagcg cttgctg
//
LOCUS       DAJTRH010000379          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTRH010000379 DAJTRH010000000
VERSION     DAJTRH010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.388"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..286)
                     /locus_tag="OO339_004323"
     CDS             complement(<1..286)
                     /locus_tag="OO339_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5928664.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPA"
BASE COUNT           84 a           83 c           90 g           90 t
ORIGIN      
        1 gggctggagt gtgttcccga cctactgatt cgcacaggta atcaacgaag cctgatacca
       61 gttttcgttc gcgaggcgtg atcgccccac cgaccggagt ccagtaatca aatcccagtt
      121 gcaggagttt gaaaaaacgc ttgtggaatg cgtagttacg cacacgctta aagtctgcgt
      181 gtatccactc acctattttg atttgatgca aaaaatcgca actctccggc gtcgccggga
      241 gaagtaatcc ggaagaggtt tgtttgacca gttgtatatg cgccatcgta gttctccgct
      301 ggcgcagtag aatgggtgtt cagcccgtta tgtagtatac cagaatt
//
LOCUS       DAJTRH010000380          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTRH010000380 DAJTRH010000000
VERSION     DAJTRH010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.389"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT          107 a           58 c           69 g          110 t
ORIGIN      
        1 atggaaagta ttgtaagttt gaccagaaat atcgttggtc gctgcattag cctgagaggt
       61 gatagccaga gtgcaagcca atgctaattg tgatactaca agtttctttt tccaggagtg
      121 catttgtcat ccctcctcag ggacgtaata agttgatcca tcaattatca atgcatagaa
      181 aacatattat gtttcgcgct aacgattatg ctgcgttaca gacgaatggt tcaattattc
      241 tttcccctaa gtccgaatgt ggacatatat aatttatgga gaaaaacacg catcaaattg
      301 ataaaaggac gatatttcag tatgttgcag ataatgttat tttt
//
LOCUS       DAJTRH010000381          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTRH010000381 DAJTRH010000000
VERSION     DAJTRH010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.390"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO339_004324"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO339_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5928665.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTRH010000382          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTRH010000382 DAJTRH010000000
VERSION     DAJTRH010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.391"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..252
                     /locus_tag="OO339_004325"
     CDS             <1..252
                     /locus_tag="OO339_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5928666.1"
                     /translation="YRNKIAIYLHWYQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLN
                     NDYWCRALSFSPTKAKNYQRYNERIKGKRQEWGILCNND"
BASE COUNT          113 a           74 c           79 g           76 t
ORIGIN      
        1 taccgcaata aaatcgccat ttatttgcac tggtatcaga aaaaaggcat cgaggttcca
       61 caaacccagc aaggggacat tggcgcgaaa gatatcccct cctggcggcg gatatgcaaa
      121 gttttactca ataacgatta ctggtgtcgg gcattatcat ttagtcctac gaaagcgaag
      181 aactatcagc gttataacga acggataaaa ggaaaacgtc aggaatgggg gatactatgc
      241 aacaacgatt aacgcaggat ttaacgcaat ttctcgccag cttgccggaa gatgatcgca
      301 tcaaagcgat taatgaaatc cgcatggcga tccatcaggt ta
//
LOCUS       DAJTRH010000383          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTRH010000383 DAJTRH010000000
VERSION     DAJTRH010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.392"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>341
                     /locus_tag="OO339_004326"
     rRNA            <1..>341
                     /locus_tag="OO339_004326"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           87 a           76 c          114 g           64 t
ORIGIN      
        1 ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa acggtagcta
       61 ataccgcata acgtcgcaag accaaagagg gggaccttcg ggcctcttgc catcggatgt
      121 gcccagatgg gattagctag taggtggggt aacggctcac ctaggcgacg atccctagct
      181 ggtctgagag gatgaccagc cacactggaa ctgagacacg gtccagactc ctacgggagg
      241 cagcagtggg gaatattgca caatgggcgc aagcctgatg cagccatgcc gcgtgtatga
      301 agaaggcctt cgggttgtaa agtactttca gcggggagga a
//
LOCUS       DAJTRH010000384          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTRH010000384 DAJTRH010000000
VERSION     DAJTRH010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.393"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(<1..>338)
                     /locus_tag="OO339_004327"
     CDS             complement(<1..>338)
                     /locus_tag="OO339_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928667.1"
                     /translation="LWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNY
                     GNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSAD
                     NVDGVYYRPL"
BASE COUNT           85 a           95 c           74 g           84 t
ORIGIN      
        1 gcagtggtcg gtaatacaca ccgtcaacgt tatccgctga gttacggccg gtatccgata
       61 tcgccatccc tgacagcccg tgtcccgaag gtgcgcaaaa tctccacgaa acggtgttac
      121 tgccggggct gtaatacatt tcatggccat accgtacatc ctgtaccccc tctgttcgat
      181 atttatagcg ggcatcgaag tttccgtaat ttaacggaat tacctgtccg ttaacagtga
      241 acactatgct gttatccggg tttctctggc tgaaaaaatg ccagcctgaa tcatcaccaa
      301 gctctgcaac aacaggcctg gatggattac cccacaaa
//
LOCUS       DAJTRH010000385          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTRH010000385 DAJTRH010000000
VERSION     DAJTRH010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.394"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>338
                     /locus_tag="OO339_004328"
     CDS             <1..>338
                     /locus_tag="OO339_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5928668.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLAD"
BASE COUNT           93 a           79 c           78 g           88 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcggatt
//
LOCUS       DAJTRH010000386          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTRH010000386 DAJTRH010000000
VERSION     DAJTRH010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.395"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..310
                     /locus_tag="OO339_004329"
     CDS             <1..310
                     /locus_tag="OO339_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928669.1"
                     /translation="REREAFLAAQQGSTSTELFTTIEGNYADAVRLLTTAHSVPFDGK
                     ATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDIISPGAFVKIGPIIRATLNR
                     "
BASE COUNT           78 a           80 c           92 g           82 t
ORIGIN      
        1 ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac
       61 caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc
      121 gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag gtatgagccc
      181 cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca
      241 tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc gcgcaacgct
      301 aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAJTRH010000387          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTRH010000387 DAJTRH010000000
VERSION     DAJTRH010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.396"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT          114 a           68 c           63 g           84 t
ORIGIN      
        1 gttatctttg gtgataataa cattattata atactgaaca gcatcataac aagaaatata
       61 acattccagt tataatattc aataatcaat gcgcgaggtg ctactaatgg tattactgcg
      121 cttacagatg atatatcaga aagcgcccgt tttagtttta atggagggtg atcttaccca
      181 ccaatagtgg acacaggact aagtgagtaa actctcaacc agaggtgact catgacaaaa
      241 ccagtatcaa tcagcaagaa gccccgtaaa caacatacgc ctgaatttcg taacgaagcc
      301 ctgaaactcg ctgaacgcat cggtgtggc
//
LOCUS       DAJTRH010000388          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTRH010000388 DAJTRH010000000
VERSION     DAJTRH010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.397"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..315
                     /locus_tag="OO339_004330"
                     /pseudo
     CDS             1..315
                     /locus_tag="OO339_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="The RecE (also known as exonuclease VIII) pathway
                     is an alternative pathway for the initiation of homologous
                     recombination in Escherichia coli. The RecE pathway is
                     activated in recB, recC sbcA (suppressors of recB and
                     recC) mutants. Activation of the RecE pathway results in
                     the production of at least two phage proteins relevant for
                     recombinational repair. RecE is a duplex DNA-specific
                     exonuclease which produces 3' overhangs in duplex DNA by
                     preferentially degrading the 5'-ending strands.
                     Recombinational repair of E.coli chromosomal DNA involves
                     as well other proteins: RecA, RecF, RecJ, RecO, RecR, and
                     RuvC.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
BASE COUNT          106 a           81 c           82 g           56 t
ORIGIN      
        1 aaccctgatc cggaaatcgt ggatattgcc tgcggtcgct cctctgcccc gatgccgcaa
       61 cgtgtaacag aagaaggaaa acaggatgat gaagaaaaaa cgcagccatc tggcgcaatg
      121 gcagatgaac aggcaacggc tgaaacaatg gaaccggatg caactgaaca tcatcagaac
      181 acgcagccgc tggatgctca gtcacaggta aattctgttg atgagaaata tcaggaacta
      241 cgggcagaac tccatgaagc ccggaaaaac attccgccca aaaatcctgt cgatgcagac
      301 aaattactgg ctgccttgag gtagc
//
LOCUS       DAJTRH010000389          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTRH010000389 DAJTRH010000000
VERSION     DAJTRH010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.398"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>325
                     /locus_tag="OO339_004331"
     CDS             <1..>325
                     /locus_tag="OO339_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5928670.1"
                     /translation="KAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDAL
                     GMSDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMK
                     NLDPIE"
BASE COUNT           99 a           64 c           93 g           69 t
ORIGIN      
        1 aaggcagttg agcaagcaat caagtcccgt gcgggttctg ctctacaggt gcaggaaaat
       61 gaagcaatca ggcagctagc acagggcgcg ggtgagataa ttgatcgcgt ttccggtgca
      121 aaagatgctc ttggtatgag cgacaagttt attgatacgg tcaatggaag aatgtctgcg
      181 ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa aggcgatgcc tgcaggtgca
      241 aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg tggcagaaga tcttggcggg
      301 atgaaaaacc ttgaccctat tgaaa
//
LOCUS       DAJTRH010000390          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTRH010000390 DAJTRH010000000
VERSION     DAJTRH010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.399"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(58..>325)
                     /locus_tag="OO339_004332"
     CDS             complement(58..>325)
                     /locus_tag="OO339_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5928671.1"
                     /translation="TRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFA
                     NMDETEFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT           86 a           94 c           69 g           76 t
ORIGIN      
        1 agccaggaat tctgacggtg cacatctgcc cccgcgccgc tttacgtaaa tccaccatta
       61 cgcaaactcc agtaactgcg cggccacatt ttcgacttcc tccggagagg aaaatttacg
      121 gaacaggatc cagttccaca gcacattcag tacagattta taaacctgct gaaactcggt
      181 ttcgtccatg ttcgcaaatg cgatagattt tgccctgcgc ccacgactac catcaggata
      241 aatatgctcg gtgtaaaatc cggcctgaat ggttacccac tcgcggaaag cgtcaaacga
      301 ctttagcaat gccgtatccc gggtt
//
LOCUS       DAJTRH010000391          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTRH010000391 DAJTRH010000000
VERSION     DAJTRH010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.400"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            112..>323
                     /locus_tag="OO339_004333"
     CDS             112..>323
                     /locus_tag="OO339_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5928672.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTART"
BASE COUNT           81 a           73 c           80 g           89 t
ORIGIN      
        1 acaggactgg cgaaaagaac ctcgtaaaaa gtattcgaat gaattcaaac ttcgggtgat
       61 gtcttcaatc gacagaagtt tctggtaatc tactggtatt taaggttttc catggctacc
      121 gttactgttc attgtccgcg ctgtcattcc gatgaagttt atcgacatgg ccgtagttgt
      181 tcccggcatg agcgtttccg ttgtcgctca tgtaaacgtg ttttccagct cacttactct
      241 tatgaagcca gaaaacccgg cgttaaggag cagatcgttg aaatggcaca taacggcgca
      301 gggggccgtg ataccgccag gac
//
LOCUS       DAJTRH010000392          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTRH010000392 DAJTRH010000000
VERSION     DAJTRH010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.401"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            1..323
                     /locus_tag="OO339_004334"
                     /pseudo
     CDS             1..323
                     /locus_tag="OO339_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           76 a           97 c           81 g           69 t
ORIGIN      
        1 ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
       61 cgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac
      121 ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt
      181 tggatgccgg gcaaccgccc gcattatggg cgttggcctc aacacgattt tccgccattt
      241 aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc
      301 tgcgcggaaa tggacgaaca gtg
//
LOCUS       DAJTRH010000393          320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTRH010000393 DAJTRH010000000
VERSION     DAJTRH010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.402"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..201
                     /locus_tag="OO339_004335"
     CDS             <1..201
                     /locus_tag="OO339_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5928673.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
     gene            204..>320
                     /locus_tag="OO339_004336"
     CDS             204..>320
                     /locus_tag="OO339_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="With FlhD is involved in the activation of class 2
                     flagellar genes and as well as a number of other genetic
                     systems; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FlhC"
                     /protein_id="HCR5928674.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGR"
BASE COUNT           89 a           78 c           81 g           72 t
ORIGIN      
        1 cttccgcaaa tggttaagct ggcagaaacc aatcagctgg tttgtcactt ccgttttgac
       61 agccaccaga cgattactca gttgacgcaa gattcccgcg ttgacgatct ccagcaaatt
      121 cataccggca tcatgctctc aacacgcttg ctgaatgatg ttaatcagcc tgaagaagcg
      181 ctgcgcaaga aaagggcctg atcatgagtg aaaaaagcat tgttcaggaa gcgcgggata
      241 ttcagctggc aatggaattg atcaccctgg gcgctcgttt gcagatgctg gaaagcgaaa
      301 cacagttaag tcgcggacgc
//
LOCUS       DAJTRH010000394          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTRH010000394 DAJTRH010000000
VERSION     DAJTRH010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.403"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT          110 a           63 c           62 g           82 t
ORIGIN      
        1 aacagggatg gactcttgtt aaccggggcg caattaaaaa tatgactgtg gaacaattac
       61 tttctttaaa ataaaaatgg agctacggat atgaacccta tgcttgatta ttgttttacc
      121 tctgcacctc agcagaccat aacacacacc ggaatcaaaa atttgttatg gataatcaaa
      181 aaaaacctcc agtagaaagg tgatcccgat ttaattccct gtgcttatgg cctgtgtgac
      241 agacaagtaa gccctgataa ggcttacact gctgaactta tttacatgca cggaaccatg
      301 atgaggaaaa agaaacg
//
LOCUS       DAJTRH010000395          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTRH010000395 DAJTRH010000000
VERSION     DAJTRH010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.404"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..110
                     /locus_tag="OO339_004337"
     CDS             <1..110
                     /locus_tag="OO339_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5928675.1"
                     /translation="DGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            107..>316
                     /locus_tag="OO339_004338"
     CDS             107..>316
                     /locus_tag="OO339_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5928676.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKA"
BASE COUNT          103 a           64 c           87 g           62 t
ORIGIN      
        1 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
       61 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaagg ttatttatga tttacccaac
      121 aaatacaggc aaaagcgggg aacaccttcg tctcaccacg ctggaaagtg tctggattca
      181 gggaaaactg cgcatgtggg ggcgctggtc gtatattggc ggcggtaaga cgggaaatat
      241 gtttaaccag ttgctggcat ccaaaaaatt gacgaaaaca gccgtcaatg aagccctgcg
      301 caggatgaaa aaagcg
//
LOCUS       DAJTRH010000396          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTRH010000396 DAJTRH010000000
VERSION     DAJTRH010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.406"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(33..254)
                     /locus_tag="OO339_004339"
     CDS             complement(33..254)
                     /locus_tag="OO339_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928677.1"
                     /translation="MPRVFMEAMPPEWQEKMAQLLFEYSDTIKTDVCGVHSCFVTAKD
                     GNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           85 a           79 c           65 g           82 t
ORIGIN      
        1 tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga ttcgatgaat
       61 tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa gcggttgttg
      121 ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt gatcgtgtcg
      181 ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat agcctccatg
      241 aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag ttgcaggtct
      301 ttacgatatt c
//
LOCUS       DAJTRH010000397          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTRH010000397 DAJTRH010000000
VERSION     DAJTRH010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.407"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(67..261)
                     /locus_tag="OO339_004340"
                     /pseudo
     CDS             complement(67..261)
                     /locus_tag="OO339_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068751.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           70 a           88 c           70 g           82 t
ORIGIN      
        1 gacaatcgca ccaataacgg ctgaagcggc gcacgaaaaa cgctttcgcg tttttcgtgc
       61 gccgcttcac ccctggttca gcatttcgtc tgcagtctga accgcaggca accatttttc
      121 agcaagtctg cgctgggaat cgctggcttt atcccatttg ctccacagtg cctcacgatc
      181 gtaatcgtgc gcttccagcg tggggcgggc gatttcaaaa tacccctagc ttgtttctat
      241 ttgctcagat tttaataaca actcagcacg tgctgccgct ggtagaactg atatatgaaa
      301 ttccaaagcc
//
LOCUS       DAJTRH010000398          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTRH010000398 DAJTRH010000000
VERSION     DAJTRH010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.408"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..>309
                     /locus_tag="OO339_004341"
     CDS             <1..>309
                     /locus_tag="OO339_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5928678.1"
                     /translation="VIGELPTYGYRRVWALLRRQAELDGMPAINAKRIYRLMRQNALL
                     LERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDRKALHW
                     "
BASE COUNT           72 a           75 c           94 g           68 t
ORIGIN      
        1 atgttatcgg agagctgccc acatatggtt atcgtcgggt atgggcgctg cttcgcagac
       61 aggccgaact tgatggtatg cctgcgatca atgccaaacg tatttaccgg ctcatgcgcc
      121 agaatgcgct gttgcttgag cgaaaactcg ctgtaccgcc atcgaaacgg gcacataccg
      181 gcagagtggc tgtgaaagaa agtaatcagc gatggtgctc tgacgggttc gagttccgct
      241 gtgataacgg agaaaaactg cgagtcacgt tcgcgctgga ctgctgtgac cgtaaggcac
      301 tgcactggg
//
LOCUS       DAJTRH010000399          307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTRH010000399 DAJTRH010000000
VERSION     DAJTRH010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.409"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            51..>307
                     /locus_tag="OO339_004342"
     CDS             51..>307
                     /locus_tag="OO339_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5928679.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAG"
BASE COUNT           95 a           53 c           83 g           76 t
ORIGIN      
        1 caaagatgtc gaagagctgc ttgaaactcg tagcgatctt ttctgaggcg atgatgaaga
       61 aaacattact tctttgtgcg tttcttgttg ggctggtaag cagcaatgta atggcattga
      121 ctctggatga agccagaact caggggcggg taggtgaaac attttatggt tatctggttg
      181 cgctgaaaac ggatgctgaa acagagaaat tagtaaccga aattaatgcc gaacgtaaag
      241 cgagttacca acaacgggca aagcaaaata atgtgtctgt agatgatatc gcgaaactcg
      301 ccgggca
//
LOCUS       DAJTRH010000400          298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTRH010000400 DAJTRH010000000
VERSION     DAJTRH010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.412"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT           92 a           52 c           60 g           94 t
ORIGIN      
        1 ataacgcatt tggtaatgat gtctgcttcc gtcaggttac ttaggttcag ttccgccatg
       61 gggggttctc gcttttatca ggacgattct gattgtacat aaaatcaata gcctggctat
      121 gatttagggc ggtgtttcaa cgtaaagtac gtagcagaga tcacattgat atctctgtta
      181 tatcttgctt gaattaatct gggcatatca gagacagata aacagaagtt acaggaacca
      241 tatcaactct aacaaaatat ataaaaatct tatagttgcg tttctttcga acaagaaa
//
LOCUS       DAJTRH010000401          296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTRH010000401 DAJTRH010000000
VERSION     DAJTRH010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.denovo.414"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
BASE COUNT           93 a           62 c           50 g           91 t
ORIGIN      
        1 gtgctctatg atctacgtag tgaaaaaatt atcagtaaag ataattactt gaccgactta
       61 aaccgcagac atcttgaact tgttcttcat actaacccca atccaagcgg ttcggtaacg
      121 aagattttac gcactatttt tactaagcag gtaaagagtt tagggccgga actctgtcat
      181 ttcttgcacc atgaactgac gaaaaaacgt tatgaagtat ggcatcacta cgatgtactg
      241 cactaccagt catcgtcatt ttctcgttca caatttaatg cattgataaa tttact
//
LOCUS       DAJTRH010000402         5822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.guided.166, whole genome shotgun sequence.
ACCESSION   DAJTRH010000402 DAJTRH010000000
VERSION     DAJTRH010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.guided.166"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            580..1395
                     /gene="sul2"
                     /locus_tag="OO339_004343"
     CDS             580..1395
                     /gene="sul2"
                     /locus_tag="OO339_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5928680.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1456..2259
                     /locus_tag="OO339_004344"
                     /note="aph(3'')-Ib"
     CDS             1456..2259
                     /locus_tag="OO339_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5928681.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2259..3095
                     /locus_tag="OO339_004345"
     CDS             2259..3095
                     /locus_tag="OO339_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5928682.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3202..3606)
                     /locus_tag="OO339_004346"
                     /pseudo
     CDS             complement(3202..3606)
                     /locus_tag="OO339_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3650..4039)
                     /locus_tag="OO339_004347"
     CDS             complement(3650..4039)
                     /locus_tag="OO339_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928683.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4257..4697
                     /locus_tag="OO339_004348"
     CDS             4257..4697
                     /locus_tag="OO339_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5928684.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5442..5591)
                     /locus_tag="OO339_004349"
     CDS             complement(5442..5591)
                     /locus_tag="OO339_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928685.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1432 a         1522 c         1519 g         1349 t
ORIGIN      
        1 tccggtaact atcaacttga gtccaacccg gaaagacacg acaaaacgcc actggcagca
       61 gccattagta acaggagtgc acgagaagat agattaagag gtcttgaagt agaggcctga
      121 ctgcggctac actgaaagaa cagttttggt aatggcggtc tcgtgaaccc tgttaccggg
      181 ttaagcagtt ccccaactga cttaaccttc gaacaaaccg cctccccagg cggtttgttc
      241 gtttacagag caagagatta cgattcattg aaaaggatct caagaagatc ctttgtagat
      301 tactatcaat taatcttccg aacatgacac cagtcactgt tgattacggt taaaccgctc
      361 cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa agaagcaaaa aagataaata
      421 tatctactta ttgagagatg atatatttat ttccaaaatg ctacagatac atcatcatga
      481 aacggatcac cgcaaacagg ttactcgcct ggggattccc tttcgacccg agcatccgta
      541 tgagactcat gctcgattat tattattata gaagccccca tgaataaatc gctcatcatt
      601 ttcggcatcg tcaacataac ctcggacagt ttctccgatg gaggccggta tctggcgcca
      661 gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg gggcagatgt gatcgacctc
      721 ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt
      781 atcgcgccgg tgctggacgc gctcaaggca gatggcattc ccgtctcgct cgacagttat
      841 caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc
      901 ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt
      961 atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg cacccgctgg cgacatcatg
     1021 gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa
     1081 cgcaaccgcc ttgtccttga tcccggcatg gggttttttc tgggggctgc tcccgaaacc
     1141 tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt
     1201 ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc
     1261 ggggccgcga cactcgctgc agagcttgcc gccgccgcag gtggagctga cttcatccgc
     1321 acacacgagc cgcgcccctt gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc
     1381 gcaagaattc gttaactgca cattcgggat atttctctat attcgcgctt catcagaaaa
     1441 ctgaaggaac ctccattgaa tcgaactaat attttttttg gtgaatcgca ttctgactgg
     1501 ttgcctgtca gaggcggaga atctggtgat tttgtttttc gacgtggtga cgggcatgcc
     1561 ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg ctggagagcg tgaccgcctc
     1621 atttggctca aaggtcgagg tgtggcttgc cccgaggtca tcaactggca ggaggaacag
     1681 gagggtgcat gcttggtgat aacggcaatt ccgggagtac cggcggctga tctgtctgga
     1741 gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac ttggcgctgt tcacagccta
     1801 tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa tgttcggacg cgccgttgat
     1861 gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg acgaggacaa gagtacgccg
     1921 cagctcgatc ttttggctcg tgtcgaacga gagctaccgg tgcggctcga ccaagagcgc
     1981 accgatatgg ttgtttgcca tggtgatccc tgcatgccga acttcatggt ggaccctaaa
     2041 actcttcaat gcacgggtct gatcgacctt gggcggctcg gaacagcaga tcgctatgcc
     2101 gatttggcac tcatgattgc taacgccgaa gagaactggg cagcgccaga tgaagcagag
     2161 cgcgccttcg ctgtcctatt caatgtattg gggatcgaag cccccgaccg cgaacgcctt
     2221 gccttctatc tgcgattgga ccctctgact tggggttgat gttcatgccg cctgtttttc
     2281 ctgctcattg gcacgtttcg caacctgttc tcattgcgga caccttttcc agcctcgttt
     2341 ggaaagtttc attgccagac gggactcctg caatcgtcaa gggattgaaa cctatagaag
     2401 acattgctga tgaactgcgc ggggccgact atctggtatg gcgcaatggg aggggagcag
     2461 tccggttgct cggtcgtgag aacaatctga tgttgctcga atatgccggg gagcgaatgc
     2521 tctctcacat cgttgccgag cacggcgact accaggcgac cgaaattgca gcggaactaa
     2581 tggcgaagct gtatgccgca tctgaggaac ccctgccttc tgcccttctc ccgatccggg
     2641 atcgctttgc agctttgttt cagcgggcgc gcgatgatca aaacgcaggt tgtcaaactg
     2701 actacgtcca cgcggcgatt atagccgatc aaatgatgag caatgcctcg gaactgcgtg
     2761 ggctacatgg cgatctgcat catgaaaaca tcatgttctc cagtcgcggc tggctggtga
     2821 tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc cgccaatatg ttctacgatc
     2881 cggctgacag agacgacctt tgtctcgatc ctagacgcat tgcacagatg gcggacgcat
     2941 tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga ccaggcgtac gcttatgggt
     3001 gcctttccgc agcttggaac gcggatggag aagaggagca acgcgatcta gctatcgcgg
     3061 ccgcgatcaa gcaggtgcga cagacgtcat actagatatc aagcgacttc tcctatcccc
     3121 tgggaacaca tcaatctcac cggagaatat cgctggccaa agccttagcg taggattccg
     3181 ccccttcccg caaacgaccc caaacaggaa acgcagctga aacgggaagc tcaacaccca
     3241 ctgacgcatg ggttgttcag gcagtacttc atcaaccagc aaggcggcac tttcggccat
     3301 ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta cagctgaaag cgaccaggtg
     3361 ctcggcgtgg caagactcgc agcgaacccg tagaaagcca tgctccagcc gcccgcattg
     3421 gagaaattct tcaaattccc gttgcacata gcccggcaat tcctttccct gctctgccat
     3481 aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg tttgctcggg
     3541 tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg ccgccacatg
     3601 aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct tagcggaaag
     3661 ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca gtgcccgtca
     3721 ctcccactct tacattgttc gcagtatcta ggtacatgtt attgcgtgca ccttcatgat
     3781 gtataatgtt attaatgaca gcagaaagat cgacagaatt aatattcttc gtacctggga
     3841 cagttatcgg gtgtcggcca gtgatgagac caaaggatgc agtataagat gaaccactac
     3901 tattatcatg gccaccatta ccaccaccgt taaaactacc tcccgaactt ccagagccgc
     3961 tatgaccatc attgtagggg tcatttggtc ccatgtgacc aaaaccaccg acagtaccgc
     4021 tatttaactc tgtactcata aaattatcct ctttgacttt taaaaacagc aaaaactgta
     4081 tttataacaa tgtgtttata tacagtattc gaaatactac gcccgtcaaa accacaccgt
     4141 caacaggttt tttgtgtatt tttcacgcct cacgggcagc ctcagacctt ttgtgttaaa
     4201 acagtaatat gttctgctgt tacaatacgg tacacacagg agacactgtc acttttatgg
     4261 catggcacac agttcaagag acactgaaat taacaggaaa gtcgcgctcg caattatatc
     4321 gtgacatggc taaaggcctt gtcagctatc gcacaggaaa aaatgatcgc cgggagttcg
     4381 agaccagtga actgattcgg gcctatggcg agctgcgcac ttctgagaca ccaaaaaggc
     4441 acaaggaggg acactccaaa tctgtcgtta gccaggcgga aaatgctcag ctagacgtca
     4501 ttcagcagaa actggacgct ctgcagcaga ctgttaccct aatgcttgaa gacaagatgg
     4561 cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga acaactacag gccgaaatta
     4621 gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg attctggtcg cggctgtttc
     4681 gctccggttc agagtaacag tgcaggtgac tctgatttac cataacatct gttatcagca
     4741 ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg gtaaaacagg caacaaacca
     4801 cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg gcatgcatga gtatgcgcac
     4861 aagtgcatag cgcggaaatt cacaccattc agccggggta agtcctgttt ccggcgttct
     4921 tcccatccga aaacggtgaa cggggacgca atttattgcg tggccgtgaa gcgttgccgg
     4981 cgcagccgga aaacccgctg tcagcacgtt caggaagggg gtgatgtaca aatggcacag
     5041 aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa gtgatgtata agtgatgtaa
     5101 aagtgatgta tgaactgaag gagcgatgta attctgtagt caggaagaat aacccgttca
     5161 tgcaggcatg aacgatacag tgaggaacgc tttttttgtc acgatctccc cttaacctgc
     5221 gtaaatcacc cgccatggtt gattattttt caacctcaaa atggtcatac acctaacgca
     5281 gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa aaaggaatat ttcgttttca
     5341 cgagtaatac ccgggtatta aaaatccact gtaattacat gaaaataaag aaataaaagg
     5401 taataccgga gtattaccac gtacgcgggc aagcgaaaaa gctataacgc ttcccggccg
     5461 acaggcgacc caaaatccac aggggcatgc aggttctctt cagtaacctg cgccattaaa
     5521 tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg aatccagttg ttcatcatat
     5581 tcagatggca tggggtgata tccagtagct gagaggttgt tagcgggttt cggggcgcag
     5641 ccctgaacca gtcatgtagc gctagcggag tgtatactgg cttactatgt tgcaacgatt
     5701 gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa ggcagcaccg gcgcgtcagc
     5761 agaatatgcg atacaggaga tattccgctt cctcgctcac tgactcgcta cgctcggtcg
     5821 tt
//
LOCUS       DAJTRH010000403         5821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.guided.167, whole genome shotgun sequence.
ACCESSION   DAJTRH010000403 DAJTRH010000000
VERSION     DAJTRH010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.guided.167"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            580..1395
                     /gene="sul2"
                     /locus_tag="OO339_004350"
     CDS             580..1395
                     /gene="sul2"
                     /locus_tag="OO339_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5928686.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1500..2258
                     /locus_tag="OO339_004351"
     CDS             1500..2258
                     /locus_tag="OO339_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5928687.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLK
                     GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSV
                     DQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQER
                     TDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDE
                     AERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2258..3094
                     /locus_tag="OO339_004352"
     CDS             2258..3094
                     /locus_tag="OO339_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5928688.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3201..3605)
                     /locus_tag="OO339_004353"
                     /pseudo
     CDS             complement(3201..3605)
                     /locus_tag="OO339_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3649..4038)
                     /locus_tag="OO339_004354"
     CDS             complement(3649..4038)
                     /locus_tag="OO339_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928689.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4256..4696
                     /locus_tag="OO339_004355"
     CDS             4256..4696
                     /locus_tag="OO339_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5928690.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5441..5590)
                     /locus_tag="OO339_004356"
     CDS             complement(5441..5590)
                     /locus_tag="OO339_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928691.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1432 a         1522 c         1519 g         1348 t
ORIGIN      
        1 tccggtaact atcaacttga gtccaacccg gaaagacacg acaaaacgcc actggcagca
       61 gccattagta acaggagtgc acgagaagat agattaagag gtcttgaagt agaggcctga
      121 ctgcggctac actgaaagaa cagttttggt aatggcggtc tcgtgaaccc tgttaccggg
      181 ttaagcagtt ccccaactga cttaaccttc gaacaaaccg cctccccagg cggtttgttc
      241 gtttacagag caagagatta cgattcattg aaaaggatct caagaagatc ctttgtagat
      301 tactatcaat taatcttccg aacatgacac cagtcactgt tgattacggt taaaccgctc
      361 cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa agaagcaaaa aagataaata
      421 tatctactta ttgagagatg atatatttat ttccaaaatg ctacagatac atcatcatga
      481 aacggatcac cgcaaacagg ttactcgcct ggggattccc tttcgacccg agcatccgta
      541 tgagactcat gctcgattat tattattata gaagccccca tgaataaatc gctcatcatt
      601 ttcggcatcg tcaacataac ctcggacagt ttctccgatg gaggccggta tctggcgcca
      661 gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg gggcagatgt gatcgacctc
      721 ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt
      781 atcgcgccgg tgctggacgc gctcaaggca gatggcattc ccgtctcgct cgacagttat
      841 caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc
      901 ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt
      961 atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg cacccgctgg cgacatcatg
     1021 gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa
     1081 cgcaaccgcc ttgtccttga tcccggcatg gggttttttc tgggggctgc tcccgaaacc
     1141 tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt
     1201 ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc
     1261 ggggccgcga cactcgctgc agagcttgcc gccgccgcag gtggagctga cttcatccgc
     1321 acacacgagc cgcgcccctt gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc
     1381 gcaagaattc gttaactgca cattcgggat atttctctat attcgcgctt catcagaaaa
     1441 ctgaaggaac ctccattgaa tcgaactaat atttttttgg tgaatcgcat tctgactggt
     1501 tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg acgtggtgac gggcatgcct
     1561 tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc tggagagcgt gaccgcctca
     1621 tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat caactggcag gaggaacagg
     1681 agggtgcatg cttggtgata acggcaattc cgggagtacc ggcggctgat ctgtctggag
     1741 cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact tggcgctgtt cacagcctat
     1801 cggttgatca atgtccgttt gagcgcaggc tgtcgcgaat gttcggacgc gccgttgatg
     1861 tggtgtcccg caatgccgtc aatcccgact tcttaccgga cgaggacaag agtacgccgc
     1921 agctcgatct tttggctcgt gtcgaacgag agctaccggt gcggctcgac caagagcgca
     1981 ccgatatggt tgtttgccat ggtgatccct gcatgccgaa cttcatggtg gaccctaaaa
     2041 ctcttcaatg cacgggtctg atcgaccttg ggcggctcgg aacagcagat cgctatgccg
     2101 atttggcact catgattgct aacgccgaag agaactgggc agcgccagat gaagcagagc
     2161 gcgccttcgc tgtcctattc aatgtattgg ggatcgaagc ccccgaccgc gaacgccttg
     2221 ccttctatct gcgattggac cctctgactt ggggttgatg ttcatgccgc ctgtttttcc
     2281 tgctcattgg cacgtttcgc aacctgttct cattgcggac accttttcca gcctcgtttg
     2341 gaaagtttca ttgccagacg ggactcctgc aatcgtcaag ggattgaaac ctatagaaga
     2401 cattgctgat gaactgcgcg gggccgacta tctggtatgg cgcaatggga ggggagcagt
     2461 ccggttgctc ggtcgtgaga acaatctgat gttgctcgaa tatgccgggg agcgaatgct
     2521 ctctcacatc gttgccgagc acggcgacta ccaggcgacc gaaattgcag cggaactaat
     2581 ggcgaagctg tatgccgcat ctgaggaacc cctgccttct gcccttctcc cgatccggga
     2641 tcgctttgca gctttgtttc agcgggcgcg cgatgatcaa aacgcaggtt gtcaaactga
     2701 ctacgtccac gcggcgatta tagccgatca aatgatgagc aatgcctcgg aactgcgtgg
     2761 gctacatggc gatctgcatc atgaaaacat catgttctcc agtcgcggct ggctggtgat
     2821 agatcccgtc ggtctggtcg gtgaagtggg ctttggcgcc gccaatatgt tctacgatcc
     2881 ggctgacaga gacgaccttt gtctcgatcc tagacgcatt gcacagatgg cggacgcatt
     2941 ctctcgtgcg ctggacgtcg atccgcgtcg cctgctcgac caggcgtacg cttatgggtg
     3001 cctttccgca gcttggaacg cggatggaga agaggagcaa cgcgatctag ctatcgcggc
     3061 cgcgatcaag caggtgcgac agacgtcata ctagatatca agcgacttct cctatcccct
     3121 gggaacacat caatctcacc ggagaatatc gctggccaaa gccttagcgt aggattccgc
     3181 cccttcccgc aaacgacccc aaacaggaaa cgcagctgaa acgggaagct caacacccac
     3241 tgacgcatgg gttgttcagg cagtacttca tcaaccagca aggcggcact ttcggccatc
     3301 cgccgcgccc cacagctcgg gcagaaaccg cgacgcttac agctgaaagc gaccaggtgc
     3361 tcggcgtggc aagactcgca gcgaacccgt agaaagccat gctccagccg cccgcattgg
     3421 agaaattctt caaattcccg ttgcacatag cccggcaatt cctttccctg ctctgccata
     3481 agcgcagcga atgccgggta atactcgtca acgatctgat agagaagggt ttgctcgggt
     3541 cggtggctct ggtaacgacc agtatcccga tcccggctgg ccgtcctggc cgccacatga
     3601 ggcatgttcc gcgtccttgc aatactgtgt ttacatacag tctatcgctt agcggaaagt
     3661 tcttttaccc tcagccgaaa tgcctgccgt tgctagacat tgccagccag tgcccgtcac
     3721 tcccactctt acattgttcg cagtatctag gtacatgtta ttgcgtgcac cttcatgatg
     3781 tataatgtta ttaatgacag cagaaagatc gacagaatta atattcttcg tacctgggac
     3841 agttatcggg tgtcggccag tgatgagacc aaaggatgca gtataagatg aaccactact
     3901 attatcatgg ccaccattac caccaccgtt aaaactacct cccgaacttc cagagccgct
     3961 atgaccatca ttgtaggggt catttggtcc catgtgacca aaaccaccga cagtaccgct
     4021 atttaactct gtactcataa aattatcctc tttgactttt aaaaacagca aaaactgtat
     4081 ttataacaat gtgtttatat acagtattcg aaatactacg cccgtcaaaa ccacaccgtc
     4141 aacaggtttt ttgtgtattt ttcacgcctc acgggcagcc tcagaccttt tgtgttaaaa
     4201 cagtaatatg ttctgctgtt acaatacggt acacacagga gacactgtca cttttatggc
     4261 atggcacaca gttcaagaga cactgaaatt aacaggaaag tcgcgctcgc aattatatcg
     4321 tgacatggct aaaggccttg tcagctatcg cacaggaaaa aatgatcgcc gggagttcga
     4381 gaccagtgaa ctgattcggg cctatggcga gctgcgcact tctgagacac caaaaaggca
     4441 caaggaggga cactccaaat ctgtcgttag ccaggcggaa aatgctcagc tagacgtcat
     4501 tcagcagaaa ctggacgctc tgcagcagac tgttacccta atgcttgaag acaagatggc
     4561 gcgggaagca gaaaaccgcc tgcgaaatga ggagagggaa caactacagg ccgaaattag
     4621 ccgtctggct gacgcgctgg cacaggaaaa aaagcgggga ttctggtcgc ggctgtttcg
     4681 ctccggttca gagtaacagt gcaggtgact ctgatttacc ataacatctg ttatcagcac
     4741 cgcctgagag gccctcaggg cgtttctgac gggaaaccgg taaaacaggc aacaaaccac
     4801 ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg catgcatgag tatgcgcaca
     4861 agtgcatagc gcggaaattc acaccattca gccggggtaa gtcctgtttc cggcgttctt
     4921 cccatccgaa aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc
     4981 gcagccggaa aacccgctgt cagcacgttc aggaaggggg tgatgtacaa atggcacaga
     5041 ggatgaaagt gacgtatgag ctgaaggaat gatgtaaaag tgatgtataa gtgatgtaaa
     5101 agtgatgtat gaactgaagg agcgatgtaa ttctgtagtc aggaagaata acccgttcat
     5161 gcaggcatga acgatacagt gaggaacgct ttttttgtca cgatctcccc ttaacctgcg
     5221 taaatcaccc gccatggttg attatttttc aacctcaaaa tggtcataca cctaacgcag
     5281 ccgcgatttt catcgcaact gcatgaatta aaagaaaaaa aaggaatatt tcgttttcac
     5341 gagtaatacc cgggtattaa aaatccactg taattacatg aaaataaaga aataaaaggt
     5401 aataccggag tattaccacg tacgcgggca agcgaaaaag ctataacgct tcccggccga
     5461 caggcgaccc aaaatccaca ggggcatgca ggttctcttc agtaacctgc gccattaaat
     5521 caaaaaggtc atattcgccg ggaagctgcg tatcagtcga atccagttgt tcatcatatt
     5581 cagatggcat ggggtgatat ccagtagctg agaggttgtt agcgggtttc ggggcgcagc
     5641 cctgaaccag tcatgtagcg ctagcggagt gtatactggc ttactatgtt gcaacgattg
     5701 cgaaaatcag tgaagtgctt catgtggcag gagaaaaaag gcagcaccgg cgcgtcagca
     5761 gaatatgcga tacaggagat attccgcttc ctcgctcact gactcgctac gctcggtcgt
     5821 t
//
LOCUS       DAJTRH010000404         5497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.guided.176, whole genome shotgun sequence.
ACCESSION   DAJTRH010000404 DAJTRH010000000
VERSION     DAJTRH010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.guided.176"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..207
                     /gene="frdB"
                     /locus_tag="OO339_004357"
     CDS             <1..207
                     /gene="frdB"
                     /locus_tag="OO339_004357"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5928692.1"
                     /translation="AHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVD
                     PAAAIQQGKVESSKDFLIATLKPR"
     gene            218..613
                     /gene="frdC"
                     /locus_tag="OO339_004358"
     CDS             218..613
                     /gene="frdC"
                     /locus_tag="OO339_004358"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5928693.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            624..983
                     /gene="frdD"
                     /locus_tag="OO339_004359"
     CDS             624..983
                     /gene="frdD"
                     /locus_tag="OO339_004359"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5928694.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1046..2179
                     /locus_tag="OO339_004360"
     CDS             1046..2179
                     /locus_tag="OO339_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5928695.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            2268..2801
                     /gene="blc"
                     /locus_tag="OO339_004361"
     CDS             2268..2801
                     /gene="blc"
                     /locus_tag="OO339_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5928696.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2798..3115)
                     /gene="sugE"
                     /locus_tag="OO339_004362"
     CDS             complement(2798..3115)
                     /gene="sugE"
                     /locus_tag="OO339_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5928697.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3291..3437)
                     /gene="ecnB"
                     /locus_tag="OO339_004363"
     CDS             complement(3291..3437)
                     /gene="ecnB"
                     /locus_tag="OO339_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5928698.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3548..3673)
                     /gene="ecnA"
                     /locus_tag="OO339_004364"
     CDS             complement(3548..3673)
                     /gene="ecnA"
                     /locus_tag="OO339_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5928699.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(3725..4291)
                     /gene="efp"
                     /locus_tag="OO339_004365"
     CDS             complement(3725..4291)
                     /gene="efp"
                     /locus_tag="OO339_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5928700.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            4333..5361
                     /gene="epmB"
                     /locus_tag="OO339_004366"
     CDS             4333..5361
                     /gene="epmB"
                     /locus_tag="OO339_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5928701.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1372 a         1396 c         1411 g         1318 t
ORIGIN      
        1 gcgcatcgtt ataacgaaga tagccgcgac cacggtaaga aggagcgtat ggcgcagttg
       61 aacagccaga acggtgtatg gagctgtact ttcgtgggct attgctccga agtctgcccg
      121 aaacacgtcg atccggctgc ggccattcag cagggcaaag tagaaagttc gaaagacttt
      181 cttatcgcga ccctgaaacc acgctaagga gtgcaacatg acgactaaac gtaaaccgta
      241 tgtacggcca atgacgtcca cctggtggaa aaaattgccg ttttatcgct tttacatgct
      301 gcgcgaaggc acggcggttc cggctgtgtg gttcagcatt gaactgattt tcgcgctgtt
      361 tgccctgaaa aatggcccgg aagcctgggc gggattcgtc gactttttac aaaacccggt
      421 tatcgtgatc attaacctga tcactctgac ggcagctctg ctgcacacca aaacctggtt
      481 tgaactggca ccgaaagcgg ccaatatcat tgtaaaagac gaaaaaatgg gaccagagcc
      541 aattatcaaa agtctctggg cggtaactgt ggttgccacc atcgtaatcc tgtttgttgc
      601 cctgtactgg taaggagcct gagatgatta atccaaatcc aaagcgttct gacgaaccgg
      661 tattctgggg cctcttcggg gccggtggta tgtggagcgc aattattgcg ccggtgatga
      721 tcctgctggt gggtattctg ctgccactgg ggctgtttcc gggtgatgcg ctgagctacg
      781 agcgcgttct ggcgttcgcg cagagcttca ttggtcgcgt attcctgttc ctgatgatcg
      841 ttctgccgct gtggtgtggt ttacaccgta tgcaccacgc gatgcacgat ctgaaaatcc
      901 acgtgcctgc gggcaaatgg gttttctacg gtctggctgc tatcctgaca gttgtcacgc
      961 tgattggtgt cgttacaatc taacgcatcg ccaatgtaaa tccggcccgc ctatggcggg
     1021 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
     1081 cgcctcttac tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
     1141 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
     1201 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
     1261 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
     1321 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
     1381 taccgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
     1441 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
     1501 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
     1561 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
     1621 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga
     1681 aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt
     1741 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
     1801 caatttaccc ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca
     1861 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
     1921 gccggtcaat cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg
     1981 cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     2041 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     2101 cgtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctgtcagat
     2161 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     2221 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     2281 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgtagtttt cctacgccgc
     2341 cgcgtggcgt gaccgtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg
     2401 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     2461 gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa
     2521 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga
     2581 aagtgtcatt ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat
     2641 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     2701 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     2761 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     2821 caataatccc caatacgatt aacgccagac tcgccagacg catcgggtta gcggactcac
     2881 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
     2941 aagccgtccc taccggtaac gatttcatcg cccaggcaag tagcgccata ctgactatca
     3001 tcgccgtcac ggtaataaca ctcggcgtca aacggctaaa gccgtgggta tatttcaggc
     3061 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     3121 gctccggaaa tttggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag
     3181 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttatga
     3241 cgatcgcaga aaaaacaaac ggcacgacac tgctgtcgta ccgttattgc ttattgctgc
     3301 gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
     3361 cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
     3421 attgtcttct tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc
     3481 aaattaataa tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaagaga
     3541 agaaaaatta gctggcagcg cgagagatgg agttaccgag atgtttgatg tcttcgccga
     3601 aaccgcaagc ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca agaacgataa
     3661 ggcgtttcat catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac
     3721 cgcattactt cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc
     3781 cgatttgtac gaacagcgga actttaacca cagcgccagt agacagggta gccggtttgc
     3841 cgccagtacc tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa
     3901 cgaagttcgg cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg
     3961 cctgatccag cagccattta gcattgtcgc caattgcttt tgcatcagca gacagctgct
     4021 cgaaagtttc gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt
     4081 tcatatcgac aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc
     4141 gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt
     4201 ttacgaattc actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag
     4261 cacgaaaatc gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag
     4321 ctaagccaca aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta
     4381 acgcaacttg ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt gaatatagac
     4441 gcggacgaaa aactgttggc cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc
     4501 cgctcattta tcgatcgcat ggagaaaggc aatccggacg atcctctttt gcgtcaggta
     4561 cttacctcgc aagatgagtt tgtcgtcgcg tccggattct ccaccgaccc actggaagaa
     4621 cagcacagcg tagtgcctgg tttgttgcat aaataccaca accgggcgct tttgctggtc
     4681 aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa
     4741 aatcagggca acaagcgtaa ctggcagact gcacttgagt atgttgctgc gcatccggaa
     4801 ctggacgaga tgattttctc cggcggcgat ccgctgatgg cgaaagagca cgagctggac
     4861 tggttgctca cacaactgga agccatcccg catatcaaac gtctgcggat tcacagccgt
     4921 ctgccgattg tgatcccggc acgtatcacc gaggcgctgg ttgaacgctt tgcccgttct
     4981 acgctgcaaa tcttgctggt gaatcacatc aatcatgcca atgaggtaga tgaaacgttc
     5041 cgtcaggcga tggctaaatt gcgccgtgtc ggtgtcaccc tgcttaacca gagcgttctg
     5101 ttacgtggtg tgaacgataa cgcacaaacg ttggcaaacc tgagtaatgc gttgttcgat
     5161 gccggcgtga tgccgtatta cctgcatgtg ctagataaag tacagggcgc ggcgcatttt
     5221 atggtgagtg atgacgaagc acggcagatt atgcgtgagt tgctgacatt ggtgtcggga
     5281 tatctggtgc cgaaactggc gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat
     5341 ctccagctac gccagcagta actgattaaa cagggaagtt aaacattaaa attgcctgat
     5401 gcgctacgct tatcaggcct acatcgtgca ttgaaattga atggatttgc aatgaactgt
     5461 aggccggata aggcgtttac gccgcatccg gcaagag
//
LOCUS       DAJTRH010000405         3720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.guided.201, whole genome shotgun sequence.
ACCESSION   DAJTRH010000405 DAJTRH010000000
VERSION     DAJTRH010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.guided.201"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            21..635
                     /locus_tag="OO339_004367"
                     /pseudo
     CDS             21..635
                     /locus_tag="OO339_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(692..2149)
                     /gene="pepD"
                     /locus_tag="OO339_004368"
     CDS             complement(692..2149)
                     /gene="pepD"
                     /locus_tag="OO339_004368"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5928702.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2411..2869
                     /gene="gpt"
                     /locus_tag="OO339_004369"
     CDS             2411..2869
                     /gene="gpt"
                     /locus_tag="OO339_004369"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5928703.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTICISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2961..>3720
                     /locus_tag="OO339_004370"
     CDS             2961..>3720
                     /locus_tag="OO339_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5928704.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVL"
BASE COUNT          893 a          950 c          984 g          893 t
ORIGIN      
        1 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
       61 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
      121 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
      181 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
      241 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
      301 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
      361 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
      421 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
      481 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
      541 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
      601 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
      661 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
      721 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
      781 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
      841 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
      901 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
      961 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
     1021 tcaccacgta gtctttaccg ctgtcgatta gtgaacggat caggcagtga atttctacgt
     1081 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
     1141 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
     1201 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
     1261 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
     1321 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
     1381 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
     1441 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
     1501 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
     1561 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
     1621 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
     1681 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
     1741 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
     1801 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
     1861 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
     1921 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa
     1981 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
     2041 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
     2101 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
     2161 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
     2221 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
     2281 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     2341 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     2401 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     2461 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     2521 tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     2581 catttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     2641 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     2701 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     2761 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
     2821 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     2881 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
     2941 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     3001 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     3061 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     3121 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
     3181 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
     3241 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
     3301 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
     3361 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
     3421 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
     3481 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
     3541 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
     3601 atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg
     3661 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
//
LOCUS       DAJTRH010000406         3252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.guided.209, whole genome shotgun sequence.
ACCESSION   DAJTRH010000406 DAJTRH010000000
VERSION     DAJTRH010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.guided.209"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            complement(92..715)
                     /gene="tetR(B)"
                     /locus_tag="OO339_004371"
     CDS             complement(92..715)
                     /gene="tetR(B)"
                     /locus_tag="OO339_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5928705.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            797..2002
                     /gene="tet(B)"
                     /locus_tag="OO339_004372"
     CDS             797..2002
                     /gene="tet(B)"
                     /locus_tag="OO339_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5928706.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2115..2708)
                     /gene="tetC"
                     /locus_tag="OO339_004373"
     CDS             complement(2115..2708)
                     /gene="tetC"
                     /locus_tag="OO339_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5928707.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2841..3212
                     /locus_tag="OO339_004374"
     CDS             2841..3212
                     /locus_tag="OO339_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5928708.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          885 a          599 c          664 g         1104 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccctc gacatcttgg ttaccgtgaa
       61 gttaccatca cggaaaaagg ttatgctgct tttaagaccc actttcacat ttaagttgtt
      121 tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt
      181 gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt
      241 ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat
      301 gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa
      361 aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta
      421 cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta
      481 aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca
      541 tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag
      601 gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct
      661 cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta gacatcatta
      721 attcctaatt tttgttgaca ctctatcatt gatagagtta ttttaccact ccctatcagt
      781 gatagagaaa agtgaaatga atagttcgac aaagatcgca ttggtaatta cgttactcga
      841 tgccatgggg attggcctta tcatgccagt cttgccaacg ttattacgtg aatttattgc
      901 ttcggaagat atcgctaacc actttggcgt attgcttgca ctttatgcgt taatgcaggt
      961 tatctttgct ccttggcttg gaaaaatgtc tgaccgattt ggtcggcgcc cagtgctgtt
     1021 gttgtcatta ataggcgcat cgctggatta cttattgctg gctttttcaa gtgcgctttg
     1081 gatgctgtat ttaggccgtt tgctttcagg gatcacagga gctactgggg ctgtcgcggc
     1141 atcggtcatt gccgatacca cctcagcttc tcaacgcgtg aagtggttcg gttggttagg
     1201 ggcaagtttt gggcttggtt taatagcggg gcctattatt ggtggttttg caggagagat
     1261 ttcaccgcat agtccctttt ttatcgctgc gttgctaaat attgtcgctt tccttgtggt
     1321 tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat acagataccg aagtaggggt
     1381 tgagacgcaa tcgaattcgg tatacatcac tttatttaaa acgatgccca ttttgttgat
     1441 tatttatttt tcagcgcaat tgataggcca aattcccgca acggtgtggg tgctatttac
     1501 cgaaaatcgt tttggatgga atagcatgat ggttggcttt tcattagcgg gtcttggtct
     1561 tttacactca gtattccaag cctttgtggc aggaagaata gccactaaat ggggcgaaaa
     1621 aacggcagta ctgctcggat ttattgcaga tagtagtgca tttgcctttt tagcgtttat
     1681 atctgaaggt tggttagttt tccctgtttt aattttattg gctggtggtg ggatcgcttt
     1741 acctgcatta cagggagtga tgtctatcca aacaaagagt catcagcaag gtgctttaca
     1801 gggattattg gtgagcctta ccaatgcaac cggtgttatt ggcccattac tgtttgctgt
     1861 tatttataat cattcactac caatttggga tggctggatt tggattattg gtttagcgtt
     1921 ttactgtatt attatcctgc tatcgatgac cttcatgtta acccctcaag ctcaggggag
     1981 taaacaggag acaagtgctt agttatttcg tcaccaaatg atgttattcc gcgaaatata
     2041 atgaccctct tgataaccca agagggcatt ttttacgata aagaagattt agcttcaaat
     2101 aaaacctatc tattttattt atctttcaag ctcaataaaa agccgcggta aatagcaata
     2161 aattggcctt ttttatcggc aagctctttt aggtttttcg catgtattgc gatatgcata
     2221 aaccagccat tgagtaagtt tttaagcaca tcatcatcat aagctttaag ttggttctct
     2281 tggatcaatt tgctgacaat ggcgtttacc ttaccagtaa tgtattcaag gctaattttt
     2341 tcaagttcat tccaaccaat gataggcatc acttcttgga tagggataag gtttttatta
     2401 ttatcaataa tataatcaag ataatgttca aatatacttt ctaaggcaga ccaaccattt
     2461 gttaaatcag tttttgttgt gatgtaggca tcaatcataa ttaattgctg cttataacag
     2521 gcactgagta attgtttttt atttttaaag tgatgataaa aggcaccttt ggtcaccaac
     2581 gcttttcccg agatctcatc tattgaaaca gcttgatagc ctttttcaac aaacaatatt
     2641 cgtgctgagt taaccagtga ttgataggta ctcttaaaat tttcttgttg atgattttta
     2701 ttttccatga tagatttaaa ataacatacc gtcagtatgt ttatggtatc atgatgatgt
     2761 ggtcgtgaca atcttaagaa catttaggtt attttatgta tattgaacag cattctcgct
     2821 atcaaaataa agctaataac atccaattaa gatatgatga taagcagttt catacaacgg
     2881 ttatcaaaga tgttctatta tggattgaac ataatttaga tcagtcttta ctgcttgatg
     2941 atgtggcgaa taaagcgggt tataccaagt ggtattttca gcggctgttc aaaaaagtaa
     3001 caggggtcac actggctagc tatattcgtg ctcgtcgttt gacgaaagcg gctgttgagt
     3061 tgaggttgac gaaaaaaact atccttgaga tcgcattaaa atatcaattt gattcccaac
     3121 aatcttttac acgtcgattt aagtacattt ttaaggttac accaagttat tatcggcgta
     3181 ataaattatg ggaattggag gcaatgcact gagagggtga tgctgccaac ttactgattt
     3241 agtgtatgat gg
//
LOCUS       DAJTRH010000407         1669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.guided.248, whole genome shotgun sequence.
ACCESSION   DAJTRH010000407 DAJTRH010000000
VERSION     DAJTRH010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.guided.248"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            <1..367
                     /locus_tag="OO339_004375"
     CDS             <1..367
                     /locus_tag="OO339_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5928709.1"
                     /translation="DSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRG
                     KGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQ
                     VSNETAMYWYWFSGAQDDA"
     gene            425..1213
                     /gene="aadA1"
                     /locus_tag="OO339_004376"
     CDS             425..1213
                     /gene="aadA1"
                     /locus_tag="OO339_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5928710.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          439 a          422 c          424 g          384 t
ORIGIN      
        1 tgactctgat gaagactctg cttgctatgg cgcattcatc gaccaagagc ttgtcgggaa
       61 gattgaactc aactcaacat ggaacgatct agcctctatc gaacacattg ttgtgtcgca
      121 cacgcaccga ggcaaaggag tcgcgcacag tctcatcgaa tttgcgaaaa agtgggcact
      181 aagcagacag ctccttggca tacgattaga gacacaaacg aacaatgtac ctgcctgcaa
      241 tttgtacgca aaatgtggct ttactctcgg cggcattgac ctgttcacgt ataaaactag
      301 acctcaagtc tcgaacgaaa cagcgatgta ctggtactgg ttctcgggag cacaggatga
      361 cgcctaacaa ttcattcaag ccgacaccgc ttcgcggcgc ggcttaattc aggagttaaa
      421 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
      481 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
      541 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
      601 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
      661 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
      721 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
      781 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
      841 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
      901 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
      961 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     1021 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     1081 gtatcagccc gtcatacttg aagctaggca ggcttatctt ggacaagaag atcgcttggc
     1141 ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg aaaggcgaga tcaccaaggt
     1201 agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     1261 ctcaagcgtt agagagctgg ggaagactat gcgcgatctg ttgaaggtgg ttctaagcct
     1321 cgtacttgcg atggcatcgg ggcaggcact tgctgacctg ccaattgttt tagtggatga
     1381 agctcgtctt ccctatgact actccccatc caactacgac atttctccaa gcaactacga
     1441 caactccata agcaattacg acaatagtcc atcaaattac gacaactctg agagcaacta
     1501 cgataatagt tcatccaatt acgacaatag tcgcaacgga aatcgtaggc ttatatatag
     1561 cgcaaatggg tctcgcactt tcgccggcta ctacgtcatt gccaacaatg ggacaacgaa
     1621 cttcttttcc acatctggca aaaggatgtt ctacacccca aaagggggg
//
LOCUS       DAJTRH010000408         1606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.guided.249, whole genome shotgun sequence.
ACCESSION   DAJTRH010000408 DAJTRH010000000
VERSION     DAJTRH010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.guided.249"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            54..527
                     /locus_tag="OO339_004377"
     CDS             54..527
                     /locus_tag="OO339_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5928711.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            622..1146
                     /gene="sat2"
                     /locus_tag="OO339_004378"
     CDS             622..1146
                     /gene="sat2"
                     /locus_tag="OO339_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5928712.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1204..>1606
                     /locus_tag="OO339_004379"
     CDS             1204..>1606
                     /locus_tag="OO339_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR5928713.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAIL"
BASE COUNT          451 a          370 c          387 g          398 t
ORIGIN      
        1 ggtgacaaaa cgagcatgct tactaataaa atgttaacct ctgaggaaga attgtgaaac
       61 tatcactaat ggtagctata tcgaagaatg gagttatcgg gaatggccct gatattccat
      121 ggagtgccaa aggtgaacag ctcctgttta aagctattac ctataaccaa tggctgttgg
      181 ttggacgcaa gacttttgaa tcaatgggag cattacccaa ccgaaagtat gcggtcgtaa
      241 cacgttcaag ttttacatct gacaatgaga acgtattgat ctttccatca attaaagatg
      301 ctttaaccaa cctaaagaaa ataacggatc atgtcattgt ttcaggtggt ggggagatat
      361 acaaaagcct gatcgatcaa gtagatacac tacatatatc tacaatagac atcgagccgg
      421 aaggtgatgt ttactttcct gaaatcccca gcaattttag gccagttttt acccaagact
      481 tcgcctctaa cataaattat agttaccaaa tctggcaaaa gggttaacaa gtggcagcaa
      541 cggattcgca aacctgtcac gccttttgta ccaaaagccg cgccaggttt gcgatccgct
      601 gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc ctgagcaggt ggcggaaaca
      661 ttggatgctg agaaccattt cattgttcgt gaagtgttcg atgtgcacct atccgaccaa
      721 ggctttgaac tatctaccag aagtgtgagc ccctaccgga aggattacat ctcggatgat
      781 gactctgatg aagactctgc ttgctatggc gcattcatcg accaagagct tgtcgggaag
      841 attgaactca actcaacatg gaacgatcta gcctctatcg aacacattgt tgtgtcgcac
      901 acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta
      961 agcagacagc tccttggcat acgattagag acacaaacga acaatgtacc tgcctgcaat
     1021 ttgtacgcaa aatgtggctt tactctcggc ggcattgacc tgttcacgta taaaactaga
     1081 cctcaagtct cgaacgaaac agcgatgtac tggtactggt tctcgggagc acaggatgac
     1141 gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg gcttaattca ggagttaaac
     1201 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     1261 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     1321 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     1381 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     1441 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     1501 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     1561 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgc
//
LOCUS       DAJTRH010000409         1606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601257
            SAMEA8900502-rid15817373.guided.250, whole genome shotgun sequence.
ACCESSION   DAJTRH010000409 DAJTRH010000000
VERSION     DAJTRH010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900502
            Sequence Read Archive: ERR6004862
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477776.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,309
            Genes (coding)                    :: 3,919
            CDSs (with protein)               :: 3,919
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 107 of 390
            Pseudo Genes (internal stop)      :: 142 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900502-rid15817373.guided.250"
                     /serovar="S. flexneri 2a"
                     /isolate="601257"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-01-10"
     gene            54..527
                     /locus_tag="OO339_004380"
     CDS             54..527
                     /locus_tag="OO339_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5928714.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            622..1146
                     /gene="sat2"
                     /locus_tag="OO339_004381"
     CDS             622..1146
                     /gene="sat2"
                     /locus_tag="OO339_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5928715.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDGDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1204..>1606
                     /locus_tag="OO339_004382"
     CDS             1204..>1606
                     /locus_tag="OO339_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR5928716.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAIL"
BASE COUNT          450 a          370 c          388 g          398 t
ORIGIN      
        1 ggtgacaaaa cgagcatgct tactaataaa atgttaacct ctgaggaaga attgtgaaac
       61 tatcactaat ggtagctata tcgaagaatg gagttatcgg gaatggccct gatattccat
      121 ggagtgccaa aggtgaacag ctcctgttta aagctattac ctataaccaa tggctgttgg
      181 ttggacgcaa gacttttgaa tcaatgggag cattacccaa ccgaaagtat gcggtcgtaa
      241 cacgttcaag ttttacatct gacaatgaga acgtattgat ctttccatca attaaagatg
      301 ctttaaccaa cctaaagaaa ataacggatc atgtcattgt ttcaggtggt ggggagatat
      361 acaaaagcct gatcgatcaa gtagatacac tacatatatc tacaatagac atcgagccgg
      421 aaggtgatgt ttactttcct gaaatcccca gcaattttag gccagttttt acccaagact
      481 tcgcctctaa cataaattat agttaccaaa tctggcaaaa gggttaacaa gtggcagcaa
      541 cggattcgca aacctgtcac gccttttgta ccaaaagccg cgccaggttt gcgatccgct
      601 gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc ctgagcaggt ggcggaaaca
      661 ttggatgctg agaaccattt cattgttcgt gaagtgttcg atgtgcacct atccgaccaa
      721 ggctttgaac tatctaccag aagtgtgagc ccctaccgga aggattacat ctcggatggt
      781 gactctgatg aagactctgc ttgctatggc gcattcatcg accaagagct tgtcgggaag
      841 attgaactca actcaacatg gaacgatcta gcctctatcg aacacattgt tgtgtcgcac
      901 acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta
      961 agcagacagc tccttggcat acgattagag acacaaacga acaatgtacc tgcctgcaat
     1021 ttgtacgcaa aatgtggctt tactctcggc ggcattgacc tgttcacgta taaaactaga
     1081 cctcaagtct cgaacgaaac agcgatgtac tggtactggt tctcgggagc acaggatgac
     1141 gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg gcttaattca ggagttaaac
     1201 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     1261 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     1321 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     1381 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     1441 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     1501 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     1561 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgc
//
